LOCUS       DAOTHD010000001        10298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_7698_length_10298_cov_3.718539, whole genome shotgun sequence.
ACCESSION   DAOTHD010000001 DAOTHD010000000
VERSION     DAOTHD010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 10298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10298
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7698_length_10298_cov_3.718539"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="P5219_00005"
     CDS             <1..1022
                     /locus_tag="P5219_00005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HRX25167.1"
                     /translation="QPRGPRPSRTLLEVREKGMITGGWAEPDRGEIMNNGIIFDIKRY
                     SIHDGPGIRTTFFLKGCPLSCWWCHNPEGMSSQPILVRHSNRCIGCGRCIESCSTGAW
                     SRSTEGRLSYDRRKCTLCGKCADACPSAAIEMAGKEMSPGDVFLEAKKDIPFYDQSGG
                     GVTFSGGEPLLQIRFLLACLHKMQEEEIHTAVDTSGYCEESTILDAAKIADLFLFDIK
                     HIDPKKHEYYTGVSNHIILSNLKKLDETLARRGRGRINIRMPLIPGINDDSENLEAVA
                     KLSASLKTLSGVNILPYHSTGEGKYRNLGMEYKMGNVLPPQDEKIAEALDIFRSQNIE
                     ADKGG"
     gene            1031..3391
                     /locus_tag="P5219_00010"
     CDS             1031..3391
                     /locus_tag="P5219_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HRX25168.1"
                     /translation="MNERIECLRKESFETSPSVSIERAVLTTEFYRENEGKYPIPILR
                     AKNFYNLCEKKTIYIGDRELIVGERGPRPKAVSTFPELNCHTAEDFEILNSRPMTRYT
                     VAGEDIRTYSEKVVPFWKGRTLRDRIFSNLPQLWLDLYEGGCFTEFMEQRAPGHTALD
                     GTIYRTGLLEFKSRIARAMENLDWINDPQATEKNEELQAMDIACDAVILFAERHAELA
                     EKMAEETTDPERKAELLKIADVCRHVPAHAPRDFHEAVQTYWFTHLGTITELNGWDAM
                     SPGHFDQHLAPFFEKGTREGTLDREKAKELLSCFWIKVNNTPAPPKVGVTAAESGTYN
                     DFTQINLGGVTEDGADGVSEVSYLLLDVLDELQLLQPQASVHISSKTPERFLRHACRI
                     MRRGSGYPSAFNSEAVVMEQLGMGKRVEDARTGGTSGCVETGSFGKEAYILHGYLNVP
                     KILELTLNNGTDPLTGKMIGIASGDPMEFHDFDDLYRAFEKQLIHVVNVKIRTDNYIQ
                     RMFARHAPAPYLSVLIHDCIKNGKDYYDGGARYNTDYIQCCGIGTVTDSLSVLKKHVF
                     QEGSIAMESLMEALKENWKGHDELRQFIWNRTPFFGNDDEYADSIMQRVYGSLLDAID
                     GKRSPKGPTYHVNMLSTTCHIYFGKKLGATPNGRFAHVPESDGTSPSHGADRRGPTAV
                     VKSLSKMNQVKSGGTLLNQRFLPSVLDGDGGLEKMAGLIRTYFNLGGHHIQFNVVDTE
                     TLRKAQKNPDEYRGLLVRVAGYSDYFVDLDDHHQEEIIARTAQESF"
     gene            3519..4535
                     /gene="dctP"
                     /locus_tag="P5219_00015"
     CDS             3519..4535
                     /gene="dctP"
                     /locus_tag="P5219_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRX25169.1"
                     /translation="MRKVTKITMLLLTAVVAITLAFPSVSSAAPEMTLRFANQVPVEH
                     TATKLMHQVADEVKEKTNGRIEIVVYPANQLGDYTLVYEELIRGTIDMAAISIPSQFD
                     PRLELTYINCFVRNFEDARKVFAPDGWLFKKMDELSSRLGVKILGFQVEGFIGIGSTK
                     PVNEPLNPNVDKGVLVRIPNMDVYKLGAEAMGYRTVTVPWAEVYTALQTGVADGVDGM
                     TPTAAYTMLKDVLKYWYDLNYSVENFNYMISQKTWDKLSEGDRKIIADACAKVTAMSI
                     DLAQQDQEKHLELMRKAGIEVHTYSAEELEPIFVKVVGTWDKLADKFSKELIDEFKAQ
                     YAPK"
     gene            4620..5183
                     /locus_tag="P5219_00020"
     CDS             4620..5183
                     /locus_tag="P5219_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRX25170.1"
                     /translation="MPDQEAMGCFPENRPDKKSPLDLALLAFFSIVTVVCSLLLSLII
                     AAAAFTRYIIGGDLYGFEEWVKLFAFWLYFMGGAYGAYNNTHVSADVVDSYMKDGLLK
                     RATVFLRNLITSGISILFTWYGYDFFMFGFMGPLGTGIALPRTTVWQIPLWTSYLAVF
                     LGLIFMTYYFTMRMLRSLRDLIQGGNS"
     gene            5180..6496
                     /locus_tag="P5219_00025"
     CDS             5180..6496
                     /locus_tag="P5219_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRX25171.1"
                     /translation="MIYIAVLILLFCLIIGVPVPMSFMASTAWLLFFGGPQAAGYQTT
                     MVLPYGFSSMNSIALISIALFIMAGGIMERGRIGEKLINLVDLLVGRIKGGLGVVGTI
                     SCAVFGSITGSACATLSCIGSIMFPRLEEAGYPRGIAAAVMSNASLLGMLIPPNSTLI
                     IFAWIGGQSILACFLATVIPGIIMTTLICLVNCWLVKDNPNVKVTEPVHGKEKIALFK
                     KRGIAALPALMLPVIVLGGIYGGIMTPTEAAAVAVVYAIPTGMFIYKGLTLRGLWDSL
                     VESAVTTGVVMVMLYSVMMLSRLYILEDLPSKMLGLFRAVSDNRWIILFMINIFLVVM
                     GMLMDDISSVTLTTPILMPIIIELGFNPIHYAAIVGVNTGLGCITPPAAPVLYLGGRL
                     GNAPINEMMSPTLWIIALAWTPTLLITTYFPRLSLFLPHFLLGTPW"
     gene            6757..6831
                     /locus_tag="P5219_00030"
     tRNA            6757..6831
                     /locus_tag="P5219_00030"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6789..6791,aa:Val,seq:gac)
     gene            6923..7312
                     /gene="acpS"
                     /locus_tag="P5219_00035"
     CDS             6923..7312
                     /gene="acpS"
                     /locus_tag="P5219_00035"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HRX25172.1"
                     /translation="MIVGLGVDLCNVERMRKSIRSDHFLKRVFHPEEITYAFSKADPA
                     RHLAGSFAAREAFSKASSVSMYSIAFCGVWVERTENGPVLKVGKPVQDLIPGGKKGYP
                     FLSISHDGEYAVAVVVIEANHEFLHFS"
     gene            7338..8807
                     /locus_tag="P5219_00040"
     CDS             7338..8807
                     /locus_tag="P5219_00040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRX25173.1"
                     /translation="METFAVSESVLMENAGSAAAEIIMNRFPSGEILVLTGPGKNGGD
                     GYVAARHLASRGRRVEILSAIPAGNLKGISEENFRRAVQCGFPVSFSPDWSDEKIAGK
                     IRHADILVDSLLGIGSKGLLRGEIARLCLHLKDTENVIALDIPTGIDPDSGHADIHCA
                     GAALTITMICPKTGIATGEGKKLAGEIIVAGVGFPVPIDAIPRIRSFSAACGKPFLPQ
                     FPYDIHKGMRGGAMVIGGCTTYRGAPLLSLRGFLRAGGGPVVLFSDEISCASCSTFLP
                     EAILENNLFSKSDGEVVSTLSSWSERIGTIVLGPGIGRSERAGEICSLVFSAWKGNII
                     IDGDGLFWLSKCAEKHHRGNILLTPHEGEAARLLGVLPEAVRKDRLASARRIAEEWGP
                     VLLKGAETLCDDGRETYLVAEGNRALAVPGSGDVLAGVIAAFLSAGTPLLESAALGAY
                     VHGKSAQSLASSNGCDGILAREIADEIPHILKEMRDPLL"
     gene            9546..10232
                     /gene="tsaB"
                     /locus_tag="P5219_00045"
     CDS             9546..10232
                     /gene="tsaB"
                     /locus_tag="P5219_00045"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRX25174.1"
                     /translation="MTRHILSINCSSARWTALGLMEGTSVAGEMNLELGKRQSSILPS
                     LVNFFLGSFSLKVDDMDYFAVVTGPGSFTGLKVAVAFTQFLSWASGEKPVIPLSSLEC
                     LAFTRLNRSKQPVCPLLWGGGGKVYSALFSLPATGEPPKEEMHPKAYSREEFQKALLA
                     SGTDTGEVLFISDTPEKCAGLFSGSFPGPFEAAVPQGAADVLLAGLYKDKAIPPRAVT
                     ARYFRDPDIG"
BASE COUNT         2558 a         2674 c         2655 g         2411 t
ORIGIN      
        1 gacagccccg aggtcctcga ccttctcgaa ccctactgga ggtccgggag aagggcatga
       61 taaccggagg atgggctgag ccggacagag gagaaataat gaacaacgga atcatattcg
      121 atataaaacg atattccata catgacggac cgggtatacg gaccaccttc tttctgaaag
      181 ggtgccccct ttcctgctgg tggtgtcata acccggaagg catgtcctct cagcccatcc
      241 ttgttcgtca ttccaaccgg tgcatcggct gcggccgttg catcgaatca tgcagtacag
      301 gcgcctggag caggagtaca gaggggcgac tcagctatga caggagaaaa tgtacactct
      361 gcggaaaatg tgcggacgcc tgtccttctg ctgcaattga gatggccggg aaagaaatgt
      421 cccccggaga cgtttttctg gaagcaaaaa aagatattcc tttttatgac caatcagggg
      481 gaggggtcac cttttcggga ggagaacctc ttctccagat tcgttttctg cttgcctgtc
      541 ttcacaaaat gcaggaagag gaaatacaca cggccgtgga cacttcaggg tactgcgaag
      601 aaagcacaat ccttgacgcg gcgaaaattg cggatctttt ccttttcgat ataaagcaca
      661 ttgacccaaa gaagcatgaa tactatactg gcgtaagtaa tcacataatt ttgtctaacc
      721 tgaagaaact cgatgaaacc ctggcccgta gaggcagagg acgaatcaat atcaggatgc
      781 cccttattcc cggcataaac gacgattccg agaatcttga agcagtggca aaactttctg
      841 catccctgaa aactctttcc ggagtcaaca ttcttccata ccactccacc ggcgagggga
      901 aatacaggaa tctcggcatg gaatacaaaa tgggaaatgt gcttccgcca caggatgaaa
      961 aaatagcgga agctctcgat atattccgaa gtcagaatat tgaagcggat aaaggaggat
     1021 aaagggtgta atgaacgaga gaatagagtg tcttcgcaaa gaaagctttg aaacgtcgcc
     1081 gagcgtttca attgaaaggg cagtgcttac cacagagttt tacagggaga acgaggggaa
     1141 gtaccccatt ccgatcctca gggctaaaaa tttctataat ctctgcgaga aaaaaacaat
     1201 atacataggt gacagggaac ttattgtggg ggaacggggt cccaggccga aagcggtttc
     1261 cacctttcct gaactgaact gccacactgc cgaagatttc gagatactca attcccggcc
     1321 catgacacga tacacagttg ccggagaaga tatcaggaca tacagtgaga aggttgtccc
     1381 gttctggaaa ggaaggactc tgagggacag gattttctca aatcttcccc agctatggct
     1441 tgacctctat gagggcggct gttttactga gttcatggaa cagcgcgcac cgggacacac
     1501 ggctcttgac gggaccattt accgcacggg acttctggag ttcaagagca gaattgcccg
     1561 cgccatggaa aatctggact ggatcaatga tcctcaggca acggagaaaa atgaggagct
     1621 gcaggcgatg gacatcgcct gcgacgctgt cattctcttt gcggaaaggc atgcggaact
     1681 cgcggagaag atggcggagg aaacgactga ccccgaaagg aaagcagagc ttctcaaaat
     1741 cgcagacgta tgccgccatg ttcctgccca cgccccccgg gatttccacg aagcggtcca
     1801 gacctactgg ttcacccacc tcggaaccat tactgaactg aacggctggg acgccatgtc
     1861 tccaggccat ttcgaccagc accttgctcc attttttgaa aaagggaccc gggaaggaac
     1921 tcttgaccgt gaaaaagcaa aggaactcct ttcctgcttc tggatcaagg taaacaacac
     1981 ccccgcaccg cccaaggtcg gcgtcacggc tgccgaaagc ggcacctaca acgattttac
     2041 ccagatcaac ctcggaggag tgacagaaga cggcgcggac ggcgtaagcg aggtttcata
     2101 cctcttgctg gacgtcctcg acgaactgca gctgcttcaa ccccaggcaa gcgtccatat
     2161 ttcatcgaaa acgccggaaa gattcctgcg gcacgcatgc cgcattatga ggcgcggctc
     2221 agggtatccg tccgccttta attcagaagc ggtggtcatg gaacagctcg gcatgggcaa
     2281 acgagttgaa gacgccagaa ccggaggaac gagcggatgt gtggaaacgg gatccttcgg
     2341 caaagaagcc tatatcctcc atggatacct caatgtgccg aaaatccttg aactgacgct
     2401 caacaacggc acagatccac tcaccgggaa aatgatcggg attgcttccg gcgatcccat
     2461 ggagtttcat gattttgacg atctgtaccg ggcgttcgaa aagcagctca tccatgtggt
     2521 gaacgtcaaa atacgtacgg acaactacat ccagcggatg ttcgcccgcc acgccccggc
     2581 cccctatctt tccgtcctca tccacgactg catcaagaac gggaaagact actacgacgg
     2641 tggcgctcgt tacaacactg actatatcca gtgctgcggc atcggaacag taacggacag
     2701 tctttccgta ctgaaaaaac atgtctttca agaaggctcg attgccatgg aatccctcat
     2761 ggaggccctg aaagaaaact ggaaaggcca tgacgaactg cggcagttca tatggaaccg
     2821 gactcccttc ttcggaaacg atgatgaata cgccgactcc attatgcaaa gggtctacgg
     2881 gtcccttctt gatgccatcg acgggaaacg ctcgccgaag gggcctacct atcatgtaaa
     2941 catgctgtcc accacatgcc acatctattt cgggaaaaaa ctcggagcga cgccgaacgg
     3001 tcgcttcgct catgtacctg aatcggatgg tacttcacct tctcacggtg ccgaccggcg
     3061 gggtcccacg gccgttgtga aatccctcag caagatgaac caggtcaaat caggagggac
     3121 tctcctgaac cagcggtttc tcccttcagt tctggacggg gatggaggcc tggaaaaaat
     3181 ggcgggactc ataagaacct atttcaacct cggaggacat catatccagt tcaacgtagt
     3241 cgacacggaa acactgcgaa aagcccagaa aaaccccgat gaatatcgcg gccttctcgt
     3301 tcgtgtcgcg ggatacagcg attattttgt cgacctggat gatcatcacc aggaagagat
     3361 aatcgccagg acagcccagg agagtttctg acgattcatc tggggaaccg ttttgtcatc
     3421 aaaagggggg attgtgaaaa agagcggcct gccggtagag gaaaaagcgc ttcggagagc
     3481 aacattcgct ggatgaaaaa tgaaaggagt gctgaataat gagaaaggtg acaaaaatca
     3541 ccatgcttct tctcactgca gtggttgcaa ttaccctggc ttttccgtcg gtttcatctg
     3601 cagcgcccga aatgaccctc cgtttcgcga accaggttcc tgttgagcat accgcaacca
     3661 agctcatgca tcaggttgct gatgaagtga aggaaaaaac aaacggccgc atcgaaatcg
     3721 tcgtctatcc tgcaaaccag ctcggagatt acaccctggt ctatgaagaa ctgatccggg
     3781 gcaccatcga catggccgcc atttccattc ccagccagtt cgacccgcgg ctcgagctca
     3841 cctacatcaa ctgcttcgtg cggaacttcg aagatgcccg caaggttttc gctcctgacg
     3901 gctggctctt caaaaaaatg gacgaactca gctcccgtct cggcgtaaag atcctcggat
     3961 tccaggttga aggcttcatc ggcatcggga gcaccaaacc ggtgaacgaa cccctgaatc
     4021 ctaacgttga caagggtgtt ctcgtacgta tacccaacat ggacgtctac aagctcggag
     4081 ctgaagccat gggctacagg acagtcactg ttccctgggc tgaagtctac acagccctcc
     4141 agaccggagt tgccgacggc gtggacggaa tgaccccaac agctgcatac accatgctca
     4201 aggacgttct gaaatactgg tacgacctca actactccgt tgagaacttc aactacatga
     4261 tcagccagaa gacatgggac aagctttccg aaggcgaccg gaaaatcatt gccgatgcat
     4321 gcgccaaggt aacggccatg agcattgatc ttgctcagca ggaccaggaa aaacaccttg
     4381 aactcatgcg caaggcgggg attgaagttc acacctattc tgccgaagaa ctggaaccca
     4441 ttttcgtaaa agttgtcggc acatgggaca agcttgctga caagttctca aaggaactga
     4501 tcgacgaatt caaggcacag tacgctccca aataaatacc cctcgggcct ctggaactgg
     4561 gcggaggagt tttcaaactc ctccgccatc atttccattg cggcggaaag gagaaaggaa
     4621 tgcctgatca agaagccatg ggctgttttc cagaaaacag gcctgacaaa aaaagccctc
     4681 tggatcttgc actgctcgct ttcttttcaa ttgtcaccgt tgtttgttcg cttctcttat
     4741 ccctgatcat tgctgccgcc gcatttacaa gatacatcat aggtggtgat ctatacggct
     4801 tcgaggaatg ggtcaagctc ttcgccttct ggctttattt tatgggagga gcctacgggg
     4861 cctacaataa cacccatgtt tctgcagacg ttgtagattc ctacatgaag gacggccttc
     4921 tgaaaagggc gaccgtcttt ctccgcaatc tgatcacgtc gggaatcagc attctcttca
     4981 catggtacgg gtacgatttt ttcatgttcg gcttcatggg gcccctcgga acagggatcg
     5041 cgcttcccag aaccacagta tggcaaatcc ccctgtggac gtcgtatctg gcagtatttc
     5101 tcggtctcat cttcatgacg tactatttca cgatgagaat gctgcgaagt ctccgggact
     5161 taattcaagg agggaactca tgatctacat cgccgttctt atccttctgt tttgcctcat
     5221 aataggcgtg cccgtaccaa tgagcttcat ggcttccacg gcgtggcttc tattcttcgg
     5281 cggcccccag gcggcaggct accagaccac catggtcctt ccctacggct tcagttccat
     5341 gaacagcatc gctctcattt ccatcgctct tttcatcatg gcgggtggca tcatggaacg
     5401 cggcaggata ggggagaaac tcatcaacct cgtcgacctt cttgtcggca gaataaaggg
     5461 cggtctaggt gtggtaggga caatatcctg cgcggtattc ggatccatta cgggttctgc
     5521 ttgtgcaacc ctttcctgta tcgggtcgat catgtttccc cgcctggaag aagcaggcta
     5581 tccgagagga atcgccgcag cagtcatgtc gaacgcatcg cttctgggga tgctcatacc
     5641 tccgaattcc acactcatca tctttgcgtg gatcggcgga cagtcaattt tggcatgttt
     5701 ccttgcaaca gttattcccg gaataataat gacaaccctc atctgcctcg tgaactgctg
     5761 gctggtgaag gacaatccca atgtgaaagt cacggagcct gttcacggaa aagaaaaaat
     5821 cgcccttttc aaaaagaggg gaatcgccgc ccttcccgca cttatgcttc cggttattgt
     5881 ccttgggggt atctacggag gcatcatgac tcccacagag gctgcggctg tggcagtcgt
     5941 ctatgccatt ccgacgggaa tgttcatcta caagggtctt acattgaggg gactgtggga
     6001 ttcgctggtg gaatcagcgg tcacaaccgg cgtcgtaatg gtcatgctct actcggtcat
     6061 gatgctgagc cggctgtaca ttctcgagga tttgcccagc aaaatgctcg gactcttcag
     6121 ggccgtctcg gacaaccggt ggatcattct gttcatgatc aacattttcc tcgtggtcat
     6181 ggggatgctc atggatgaca tcagcagcgt gacgctgacc acgccaatcc tcatgcccat
     6241 aatcatcgaa ctcggattca atccaatcca ttacgcggcg atcgtaggcg tcaacaccgg
     6301 tcttggctgc ataacacctc cggcggcgcc ggtcctgtac ctcggaggaa gactgggcaa
     6361 cgcaccgatc aacgagatga tgtcgccgac attatggatc atcgccctgg catggacgcc
     6421 gactttgctc attaccacct attttcccag gctttctctt ttcctgcccc atttcctcct
     6481 ggggacaccc tggtgaaaaa ggctgcaaag gaaaaatgtt ttatcgaaat ctcttcggtt
     6541 ttctgtgtct ttgctgtttt atcctgggac tctggaccaa gtcaccctat gcatactggg
     6601 gtgcccttgt cagcggtaca ctctcaaggg ctgcggtccg gaagggttgg aacccaccga
     6661 aatggctcac atctttattc cgttggtccg aatagccccg ggggaaatct gagttattga
     6721 caaacagtct cgaggtctgt aaaatccttt tctgttgggt ggttagttca gaggaagaac
     6781 gcttccttga cgcggaagag gtcacaggtt cgagtcctgt accacccacc attattaaga
     6841 gtaaaggggt tccggttttg ccggaacctc ttttttatgg tataaccttc gtaaaaatca
     6901 aatttcgcgt ggaggcaaga acgtgattgt tggactggga gtcgatctct gcaacgttga
     6961 aagaatgcga aaatccatcc ggtccgacca tttcctgaag agggtcttcc atcctgaaga
     7021 aataacctac gccttttcga aggccgatcc cgccaggcat cttgccggaa gtttcgccgc
     7081 aagggaggcc ttttcaaaag cttcttcggt ttccatgtac tcaatcgcct tttgcggggt
     7141 ctgggtagaa cggacggaaa acggtccggt tctcaaagtc ggaaaaccgg ttcaggattt
     7201 aattccgggc ggaaaaaaag gatacccctt tctctccatt tctcacgacg gagaatatgc
     7261 cgttgcagtg gtagtcatcg aggcaaatca tgaatttctt cacttcagct gatatcagaa
     7321 gaatagaaaa tgaaatcatg gaaaccttcg cggtttccga atctgttctt atggaaaacg
     7381 cgggttccgc cgctgcagaa atcataatga atcgttttcc gtcaggcgaa atcctcgtgc
     7441 tcaccggacc ggggaaaaac ggcggcgacg gatacgtcgc cgccagacac ctcgcttcga
     7501 gagggaggag agtcgaaatt ctttcggcga ttcctgccgg aaacctcaaa ggaatttcgg
     7561 aagagaattt ccgtcgtgcc gttcaatgcg gttttcccgt ttccttttca ccggattggt
     7621 ccgacgaaaa gatcgccggg aagattcggc atgcagacat cctggtggac tctcttctcg
     7681 gtataggatc gaaggggctc ctgagaggtg aaatcgcaag gctttgtctt catttaaaag
     7741 atacagaaaa tgttatagcg ttggatattc caaccgggat tgaccctgac tcgggacacg
     7801 ctgacatcca ttgtgcggga gcagccctga ccatcactat gatttgcccc aaaacaggaa
     7861 tcgcgacagg agagggcaaa aagctcgcag gagaaatcat cgtagccggt gtgggttttc
     7921 cggtaccgat cgacgccata ccccgtatcc ggtctttttc tgcggcgtgc ggaaaaccgt
     7981 ttcttcccca gtttccttac gacatccaca aaggtatgcg gggtggcgct atggtaatcg
     8041 gcgggtgcac aacctacagg ggagcccccc tgctctccct ccgcggcttc ctgagagcgg
     8101 gcggagggcc cgttgttctc ttttccgatg aaatttcatg tgcttcatgt tctaccttcc
     8161 tcccggaggc gatccttgaa aacaatcttt tctcgaaaag cgacggggaa gttgtatcaa
     8221 ctctttcgag ctggtcggag agaataggaa cgatcgttct cggcccaggc ataggccggt
     8281 cggagagggc aggagaaatc tgcagccttg ttttttctgc gtggaaaggc aacatcataa
     8341 tcgatggtga cgggttgttc tggctttcaa aatgtgcaga aaagcaccac cggggaaata
     8401 ttctccttac tccccatgaa ggggaagcgg cacggcttct tggtgttctg cctgaagctg
     8461 tgcgaaaaga ccgccttgca tcggcgagaa ggatagcgga ggagtggggg ccggttcttc
     8521 ttaagggagc cgaaacactg tgcgacgacg gacgggagac ctatctcgtt gcggaaggca
     8581 atcgtgcgct ggctgtcccc ggttcagggg acgttctcgc cggagtgatc gccgcctttc
     8641 tgtccgcagg gactcctctc cttgaaagtg cggcccttgg cgcatacgtc cacggaaaat
     8701 cggcccaatc actggcctca tcgaacggat gcgacggaat cctcgctcgg gagattgctg
     8761 acgaaatacc ccatattctg aaagaaatga gagacccgct attgtgagaa gaaaaagaat
     8821 cgactgggat gaagaactca aaaaaacaga gagaatccga ttaaagggat ttcgttatac
     8881 tgccgccggg tttgtccttg ttcttttctg gctccttgga gtacggcaaa tacagaaaga
     8941 aatcccgatt cttccaatgc ttttttcttt ttttctcgtc atttcgggtg ttcttctctt
     9001 aatcattgtt cttcgcagaa aaaaatagag gagtggtgac cggacatgaa gtttctgcca
     9061 ccccgaacgg tgattacgga atccgaagag gaaacgcgct gtctcggggc actgctttca
     9121 aatcacattt ccggcggtat tacgattctt ctttccggag atctgggctc aggaaaaacc
     9181 acccttgtga gagggttttg cgagaacctc ggcttcagga aggtacgaag cccatccttc
     9241 acgcttgtaa accattacat ggcagggggc cgaaggatcg tccattcgga cctctacaga
     9301 cttgaatccc gggacgtcag cgaacttgac ctggaggaag acgactccgg cgatactgta
     9361 cttttcgttg aatgggcaga aaagaccgcc tttttttccg aaaggccttt atggagaata
     9421 aatatctcag ctccggacgc atttctctct ccagaacggc ggcaatgtgt tttttttcct
     9481 ctcaacagcg agggagaggt ttttctttcc cgattcttgc aggaagcaag agaggtgatt
     9541 cccctatgac ccgtcacatc ctgtcgatca actgttccag tgcacggtgg accgccctcg
     9601 gtcttatgga gggaacatcc gttgcagggg aaatgaatct tgaactggga aaaagacaat
     9661 cctcgattct accgtctctt gtcaattttt ttctggggag tttttctctc aaggtggacg
     9721 atatggacta ttttgctgtg gtcacaggcc ccggttcttt taccggcctg aaggttgctg
     9781 tggcattcac ccagttcctt tcctgggcat ccggcgagaa gccggttata cccctttctt
     9841 ccctggagtg tctggcattc accagactga acaggtcgaa gcagcccgtc tgtccccttc
     9901 tatggggcgg aggcggcaag gtttattcgg ccctcttcag ccttccggca acgggggagc
     9961 ccccgaagga ggaaatgcat ccgaaggcct acagccggga ggagtttcaa aaggccctcc
    10021 ttgcgtcagg aactgacacc ggggaagttc tgttcatttc cgatacacct gaaaaatgtg
    10081 ccgggctgtt ttccggcagc tttcccgggc catttgaagc cgcggttccc cagggagctg
    10141 cagatgtcct tctcgccgga ctctacaaag acaaggccat accaccccgg gctgtcacag
    10201 cccgatattt ccgtgacccg gatatagggt aaagggtttt tatctttttt atcccttttg
    10261 aaaagtggag ataaaaaaga taaattttat ttcgcggt
//
LOCUS       DAOTHD010000002         9543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_8537_length_9543_cov_3.295110, whole genome shotgun sequence.
ACCESSION   DAOTHD010000002 DAOTHD010000000
VERSION     DAOTHD010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 9543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9543
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8537_length_9543_cov_3.295110"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="P5219_00050"
     CDS             <1..851
                     /locus_tag="P5219_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HRX25175.1"
                     /translation="IGFFVWFLRKFKRMPTSFRGFFNLFVLSGVSTVVVGALTGSWFG
                     DLVVAFGIFSFLRPLKNSLIILNPMEDPMTFLGLSLALGVVQLLFGLGIAFYDALRKK
                     NYMGAFADTGGWIVFLVGLLLWGGCAGGFLSTGTEPLAKTLTIAGAVILLATQGREKT
                     GFLSKAVSGLLSLYGVTSYLGDVLSYSRLLALGLATSAVGVIINMLAGLAGDIPFIGW
                     ILALLLIIGGHIFSIAVNVLGAFVHSLRLQYVEFFSKFYTGGGRVFAPLTYKTSYVSI
                     KKEPVE"
     gene            884..1363
                     /locus_tag="P5219_00055"
     CDS             884..1363
                     /locus_tag="P5219_00055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HRX25176.1"
                     /translation="MDLLGIALAILGAAMAAGWAGAGSAIGVGIAGESGAGVMTEDPG
                     KFGLVLLLQALPGTQGIYGLLIAFFAILKVGLLGGGAEISVNVWQGLAIFFACLPIAV
                     GGYFSAIAQGKTSAACIQMIAKRPEETGKAVILPAMVETYAVLSLLMSIILLNGIKL"
     gene            1386..1967
                     /locus_tag="P5219_00060"
     CDS             1386..1967
                     /locus_tag="P5219_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HRX25177.1"
                     /translation="MSLAEIKKKIEADAQEEAGKILEKARSVVESINKEADGEIKKIE
                     DTYGERFRKEQPEILRRREIVAALDVKKIELGQKQKTIADAFSQALSQLSILPGEKYL
                     SLVGNLLLKATETGDETVIVSNKEKHISQEWLAGFNEKHGKKLVLGEEKAPISGGFIL
                     KKDAIETNCSWEMLIRWIRDDIEAGVVKRLFSA"
     gene            1984..2997
                     /locus_tag="P5219_00065"
     CDS             1984..2997
                     /locus_tag="P5219_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HRX25178.1"
                     /translation="MAPAEQYGYAVARLRAMSGRLLEEALLQRILESEDLDSALKVLS
                     ETVYSSWLMELKGSGEFDRAIEAELLHVYGEVQKFVPDTALVQLCRLPYDFHNVKVLM
                     KSALLVREGGERRFDLLTSLGNVPVDDLIMALESEDFRLLPFGLHQLVPHCFSVWEQS
                     KDILEVEKLLDGGMFSAMRKLGAATGIEAASMWIRGKIDAENVRNLLRMKRMDMETGA
                     VAGFLHDGGLISREKLLSLFSEPVESWSKILSFADVSKAFSHVQEINNLNSLVVEMEK
                     VLDEYVADIVERAKYLAFGPENVISYLWKKEMEAKNLRIALVSVSNDTDRTLARGLFR
                     HVG"
     gene            2987..3328
                     /locus_tag="P5219_00070"
     CDS             2987..3328
                     /locus_tag="P5219_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HRX25179.1"
                     /translation="MSVKNTGHPMAAVGEYETVLPFQAVGVRPFIIKDNTPSAVEETL
                     LGLARDKYAVVFVQESCYVASSQFIADLNSEYETSFIPIPGLKGSLGIGIESIRSSVE
                     RAVGMDIFAVK"
     gene            3362..5155
                     /locus_tag="P5219_00075"
     CDS             3362..5155
                     /locus_tag="P5219_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRX25180.1"
                     /translation="MATEKTIRGTIEKISGPLVVAKGMFGASMFDVVRIGDIGLVGEI
                     IELKGDTASIQAYEETSGLMPGEPVVSTGEPLSVELGPGLMEQFYDGVQRPLNLIEDV
                     AKSHFISRGIDVPAIDRKTKWQFTPKVKEGDFVAEGDVLGTVQETVLVEHRVMVPVGT
                     SGKVTKIKEGSFTVEETIAVLEKDGEKKNIAMLQRWPVRKARPVAKRLPPVIPLTTGQ
                     RVVDAFFPIAMGGTACVPGPFGSGKTVIQHQLAKWAEAEIVVYVGCGERGNEMTDVLL
                     EFPELEDPRSGQPLMKRTVLIANTSNMPVAAREASIYTAITMAEYYRDMGYSVALMAD
                     STSRWAEALREMSGRLEEMPGEEGYPAYLGTRLASFYERAGRAVVLGNHGREGSVTAI
                     GAVSPPGGDLSEPVSQNTLRVTKVFWGLDANLAYQRHFPAINWLLSYSLYTEKLDEFW
                     DTKFDDEWSSLRIEGMTLLEEEDKLREIVRLVGIDALSKEERMVLETAKSLREDFLHQ
                     NAFHEVDTYASMEKQFKMLKTIISFHTLGMEALKKGASMNEVFKLPVRESIARMRYLE
                     EKDIGRIDELEETIRKEINGVIPVGGESDAA"
     gene            5145..6563
                     /locus_tag="P5219_00080"
     CDS             5145..6563
                     /locus_tag="P5219_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HRX25181.1"
                     /translation="MLPKEYGTISELSGPLLMVEKTQNVRYDELVEIELASGERRRGR
                     VLEITSDKALVQVFEGTDGIDIKSTKLLFLGKVLTLPVSKDMLGRVFNGRGEPIDDGA
                     PILAEKKLDINGMPMNPFSRDFPSEFIQTGISTIDGMNPMVRGQKLPIFSGSGLPHNR
                     MAAQIARQATVISGHEDFAVVFAAMGITFEEASFFMEDFRKTGAIQRTVMFVNLADDP
                     AIERITTPRIALTAAEYLAFEHDMHVVVILTDLTNYCEALREISAARKEVPGRRGYPG
                     YLYTDLATMYERAGRLKGKSGSITQIPILTMPEDDKTHPIPDLTGYITEGQIILSRTL
                     HRKGIYPPVDVMPSLSRLKDKGIGKGKTREDHADLMNQLFAAYARGKEAKELAVILGE
                     GALTDEDKAFAKFADLFEDQYVRQGEYQNRTFEETLELGWNLLTIIPVKELKRVRDAY
                     IEKYLAPRLKNGERAETVPGKV"
     gene            6576..7199
                     /locus_tag="P5219_00085"
     CDS             6576..7199
                     /locus_tag="P5219_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HRX25182.1"
                     /translation="MARLNVNPNRMELSRLKKRLVVAKRGHKLLKDKQDALIKAFLER
                     AREGKVLREELESELAECYKSFLLARAQTLPAMLEQALMISGAKCDLHVGMQNVMSVL
                     VPVYSVEQGGSPVNYGLATSLGSLDVALERFAALIPRLIALAAKEKAINLMAVEIEKT
                     RRRVNALEHVLIPGFVETIRYITMKLDEQERSTLSRLMKIKEIVRSH"
     gene            complement(7252..8058)
                     /locus_tag="P5219_00090"
     CDS             complement(7252..8058)
                     /locus_tag="P5219_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HRX25183.1"
                     /translation="MIEKSSYDSPQYDIFKANVKKLTGLDLNSYKNQIHRRVHMLMQR
                     WNVTEYDLYFKTMKENDQKLREFLDYLTINVSEFFRNPAKWTELRETIIPRLIQARGK
                     KQLKLWSAGSATGEEPYSLSILSLDAKLAAQSPVLASDIDQGAISIAKKGTYLKRQIV
                     NVPPELVSKYFTTEDGGETYSVTQEVKGRVNFQRLNLIEDTFAEDFDLILCRNVVIYF
                     SAETKTALYHKFFNALRPGGYLLVGSTEQIFEYRKIGFESAGAFLYQKPV"
     gene            8168..8499
                     /gene="rnpB"
                     /locus_tag="P5219_00095"
     ncRNA           8168..8499
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="P5219_00095"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            8633..9058
                     /gene="mraZ"
                     /locus_tag="P5219_00100"
     CDS             8633..9058
                     /gene="mraZ"
                     /locus_tag="P5219_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRX25184.1"
                     /translation="MLVGTYEHKIDSKARIVLPSKFREELGECVISTIGIDRCVSLYS
                     RSRWENVMTKLQDLPFSKSKTRGLLRVMLSSAHELPVDSAGRILLPPVLRQHGDLSQD
                     VVFVGVNDHVELWDRDRWNEYREEMVGELSEIAEGVEGF"
     gene            9055..>9543
                     /gene="mraW"
                     /locus_tag="P5219_00105"
     CDS             9055..>9543
                     /gene="mraW"
                     /locus_tag="P5219_00105"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HRX25185.1"
                     /translation="MTEHIPVLIAEILAFLQTRPKVGKVLDCTLGLGGYSEAVLETFP
                     EVQVWGLDQDDEAIRIASDRLEPFGDRFHPIHGNFAEAAELAGNAGPFDAILFDLGVS
                     NLQISEGYRGFSFQSDGPLDMRMNRHFSLTAEYLVNNWTEKELSDIFWKYGEERYSRQ
                     IAR"
BASE COUNT         2365 a         2404 c         2612 g         2162 t
ORIGIN      
        1 tgatcgggtt tttcgtttgg tttctgagga aattcaagag aatgcccacc agtttcaggg
       61 ggtttttcaa tctcttcgtc ctttccggag tttccacggt tgtcgtaggt gcgctcacag
      121 gcagctggtt cggagatctc gtggttgcct tcggtatttt ctcttttctc cgccccttga
      181 aaaactctct aataatcctc aatccaatgg aagatcccat gacattcctt ggtctttccc
      241 ttgctctcgg agttgtgcag cttcttttcg ggcttggaat cgctttctac gatgctcttc
      301 gaaaaaagaa ttacatggga gcctttgccg ataccggcgg ttggatagtc tttcttgtcg
      361 ggctgcttct gtggggaggg tgcgcaggcg gttttttgtc caccggcacg gaacctctgg
      421 ccaagacgct cacaatagcc ggtgcagtca tcctgctcgc cacacagggg agggagaaga
      481 caggattcct gtcaaaagca gtctccggcc tgttgagtct ctacggcgtc acctcctatc
      541 ttggtgacgt cctcagctac agccgtcttc tcgccctcgg cctggccaca tcggcagtcg
      601 gagtaatcat caacatgctt gccggcctgg ccggagatat cccgttcatc ggatggatcc
      661 tcgctctcct gcttatcatc ggagggcata tattcagcat cgcggtaaat gtcctcgggg
      721 catttgtcca ctccctgaga cttcagtacg tagagttctt cagcaaattc tatacaggag
      781 ggggacgggt cttcgctccc ctgacctaca agacaagtta cgtttcaatc aagaaagagc
      841 ctgtcgaata atttctatag cttgaaagga gcgtgtcaat actatggatc ttcttggaat
      901 cgcattggca atacttggag cagctatggc cgcaggatgg gcaggagcgg ggtctgccat
      961 cggagtcggg attgccggag aatcgggagc cggcgtcatg accgaagatc cggggaaatt
     1021 cggccttgtc ctccttctgc aggctcttcc cggcacacag ggaatttatg ggctccttat
     1081 cgctttcttc gccatcctca aggtagggct tcttggaggc ggcgccgaaa tcagcgtcaa
     1141 cgtgtggcag gggctggcaa tcttcttcgc ctgccttccc attgctgtcg gaggttactt
     1201 ttcggccatc gcccagggca aaacctctgc ggcgtgcatc cagatgattg caaaacgccc
     1261 cgaagaaaca ggaaaggccg ttatcctccc tgcgatggtc gaaacatacg ccgttctttc
     1321 tcttctgatg agcatcattc tcctcaacgg aatcaagctc taattctgtc aagggggtaa
     1381 attcaatgtc cttggcggag ataaagaaaa aaatagaagc ggacgcacag gaggaagcgg
     1441 gaaagattct tgaaaaagcc cgttccgtag tggagtctat caacaaggaa gccgacggtg
     1501 aaatcaagaa aattgaagat acctacggcg aaagattccg taaagaacag ccggaaatcc
     1561 tcagaagaag agagatcgtg gcggccctcg atgttaagaa gatcgagctt ggccagaaac
     1621 agaagaccat tgccgatgct ttctcccagg ccctttccca gctttcaatc cttcctggag
     1681 aaaaatatct ctctctggtg ggaaaccttc tcctgaaagc gacggaaaca ggagatgaga
     1741 cagtgatagt ttcaaacaag gagaagcata tctcgcagga atggcttgcc ggattcaatg
     1801 aaaagcatgg gaaaaaattg gttctcggag aggaaaaagc gcccatttcg ggtggtttta
     1861 tcctgaaaaa agacgctatc gagacgaatt gctcctggga aatgctcatt cgctggatac
     1921 gggacgatat tgaggcaggc gttgtcaaac ggctgttttc agcctagccg gaggtgatgc
     1981 tccatggctc cggcagagca gtatgggtat gcggttgcgc ggctccgggc aatgtccgga
     2041 cgccttcttg aagaggctct tttgcagcgt attctggaaa gcgaagacct tgactcggcg
     2101 ctgaaagtcc tcagtgaaac agtctattcc agttggttga tggagctgaa aggcagcggt
     2161 gaattcgaca gggccatcga agcggagctt cttcatgtct atggggaagt acagaaattt
     2221 gttcctgaca cggccctggt ccagctctgc cgcctgccct atgattttca caatgtcaaa
     2281 gttcttatga agagcgctct cctggtccgc gaaggaggag aacgacgatt cgatctcttg
     2341 acctctctcg ggaacgtccc ggttgatgac cttatcatgg ccctggaaag cgaagatttc
     2401 cgcttgcttc ccttcggtct tcatcaactc gttcctcatt gtttttccgt ttgggagcag
     2461 tcaaaagaca ttctcgaagt ggaaaagctt cttgacgggg gcatgttctc agccatgagg
     2521 aagctagggg ctgcaacggg cattgaagcg gcatcgatgt ggattcgggg aaagatcgac
     2581 gcggaaaacg tccggaacct gctgcggatg aaaagaatgg atatggaaac tggagcagtc
     2641 gccggttttc ttcacgacgg aggccttatt tccagggaaa agcttctctc cctcttttct
     2701 gagccggtgg aaagctggag caaaattctt tccttcgcag atgtgtcaaa agcgttttcc
     2761 cacgttcagg aaatcaacaa cctgaattca ctcgttgtcg agatggaaaa agtgctcgat
     2821 gagtacgtcg cggacattgt ggaaagggca aaatacctgg ctttcggccc ggagaatgtc
     2881 atttcttacc tctggaaaaa agaaatggaa gcgaaaaatt tacgaatagc tctggtttcc
     2941 gtctcgaatg atacggacag gacgctggca agggggctgt tccgtcatgt cggttaagaa
     3001 tacaggacat cccatggcag cggtcggtga gtacgaaacg gttcttccgt tccaggcagt
     3061 gggagtgcgg cctttcatca tcaaggacaa cactccttcc gccgtggaag aaaccctcct
     3121 cggcctggcc cgcgataagt atgctgtcgt cttcgttcag gaaagttgtt acgtagcttc
     3181 ttcccaattc atagccgacc tgaacagtga atacgaaacg agttttatcc ccatccccgg
     3241 actcaaaggc agtctgggaa taggtattga atccatacgg agcagcgtgg aacgcgctgt
     3301 aggcatggat atctttgcgg tgaaataatg cagaatacgg ttcgtctgga ggcgatagac
     3361 tgtggccact gaaaaaacta tacgggggac catagaaaag atttccggac cactcgtggt
     3421 ggcgaagggc atgttcggcg ccagcatgtt tgacgtggtt cggatcggtg acattggtct
     3481 cgttggagaa ataattgaac tcaagggaga cacggcctcc atacaggcct acgaggaaac
     3541 ttcagggctt atgcccggag agccggtggt aagcacgggc gaacctctga gcgtcgagct
     3601 tggccccggc ctcatggagc agttttacga cggagtccag cgtcccctga acctcattga
     3661 ggatgtggcg aaaagccatt tcatctcgag aggcatcgac gttcctgcca tcgacaggaa
     3721 aacgaaatgg caattcacgc cgaaagtgaa ggaaggcgat ttcgtcgccg agggagatgt
     3781 tctcggcacc gtacaggaga cggtccttgt cgaacatcgt gttatggtgc ccgtcggcac
     3841 ttccggcaaa gtcacaaaaa tcaaggaagg cagtttcacc gttgaagaaa ctattgctgt
     3901 actggagaag gacggtgaaa agaaaaacat cgccatgctc caacggtggc ctgtacggaa
     3961 agcccgacct gtagctaagc ggctccctcc cgttatccct ttgacaacag ggcagagagt
     4021 ggtggacgcg ttcttcccta tcgccatggg tggaaccgcc tgtgtcccgg gccccttcgg
     4081 atcagggaaa acggtgatcc agcaccagct cgcaaagtgg gccgaagctg aaattgtcgt
     4141 ttacgtggga tgcggcgaac ggggcaatga aatgaccgac gtgctgctcg aattccccga
     4201 acttgaagac ccaaggtcgg gacagcctct catgaagcgg accgtcctca tcgccaacac
     4261 ctcaaatatg cccgtggcgg ccagggaagc cagcatttac acagccatca ccatggctga
     4321 atactacagg gacatggggt attccgtcgc tctcatggca gactccacga gcaggtgggc
     4381 agaggctctc cgtgaaatgt cgggccgtct tgaagaaatg cccggggaag aaggataccc
     4441 ggcctacctc ggaacacggc ttgcgtcatt ctatgaaaga gcgggacggg ctgtggttct
     4501 cggaaaccac ggaagagaag gctccgtaac agccataggc gccgtttcac ctccgggcgg
     4561 cgacctttca gaaccggtca gccagaatac gctcagggtc accaaggtct tctggggact
     4621 ggatgcgaac ctcgcatacc aaaggcattt cccggcgatc aactggctgc tgagctattc
     4681 cctttatacc gaaaagcttg atgaattctg ggacaccaag ttcgacgacg agtggagcag
     4741 cctccgcatc gaaggtatga cccttttgga agaagaggac aaactccggg aaatcgttcg
     4801 gctcgtcggc atcgatgccc tttccaagga agagcgcatg gtacttgaaa cggcgaaatc
     4861 cctgagggaa gacttccttc accagaacgc cttccatgaa gtggatacct atgcttccat
     4921 ggaaaagcag ttcaagatgc tgaagacaat tatctccttc catactcttg gaatggaagc
     4981 cctgaaaaag ggagcgtcca tgaacgaggt attcaagctt ccggttcgtg aatccatcgc
     5041 cagaatgcgc tatcttgagg aaaaggacat cggcaggatc gacgaattgg aagagacgat
     5101 acggaaggaa atcaacggcg tcattcccgt gggaggtgaa agcgatgctg cctaaggaat
     5161 atgggaccat aagcgaactg tccggtcccc ttctcatggt ggaaaaaacg cagaacgttc
     5221 ggtatgacga acttgtcgaa attgagctcg cctcaggaga acggaggcgg ggcagggttc
     5281 ttgaaataac gtccgacaag gcccttgttc aggttttcga aggaaccgac ggaatagaca
     5341 tcaagagcac caagctgctc ttcctgggca aagtgctgac tcttcccgtc agcaaggaca
     5401 tgctcggacg ggtattcaac ggccggggcg aacctattga cgacggagcc ccgatcctcg
     5461 ccgagaagaa gcttgacatc aacggaatgc ccatgaatcc tttctcccgc gacttcccct
     5521 ccgaattcat ccagacgggc atctccacca tcgacggaat gaaccccatg gttcgcggac
     5581 agaaacttcc cattttctcg ggaagcggcc tgccgcacaa caggatggct gcccagattg
     5641 ccaggcaggc aacggtcatc agcggccacg aggattttgc cgtggtgttc gcggccatgg
     5701 gaatcacgtt cgaggaagcg tccttcttca tggaggactt caggaaaacg ggcgccatcc
     5761 agaggacggt catgttcgtc aaccttgccg acgacccggc aatcgaacgg ataacaaccc
     5821 cccgcatcgc gcttacagca gcggaatatc tcgcttttga acatgatatg cacgtggtgg
     5881 tcatcctgac cgacctcaca aactattgcg aggccctccg ggaaatttcc gcggcgcgga
     5941 aggaagttcc cggccggaga ggctacccgg gttacctcta caccgacctc gccaccatgt
     6001 acgaacgagc gggacgtctc aagggaaaga gcggctccat cacccagatt cccattctca
     6061 ccatgcccga ggatgacaaa acccacccga tccctgacct tacagggtac atcaccgagg
     6121 gacagatcat cctgagcaga actcttcaca gaaagggcat ttatcccccc gtggacgtca
     6181 tgccgtccct ttcacgactg aaggacaagg gaataggaaa gggaaagacc agggaagacc
     6241 acgcagacct tatgaaccag cttttcgccg cctacgcaag aggcaaggaa gccaaggaac
     6301 tcgcggtcat tctcggagag ggcgctctta cagatgagga caaggccttc gcgaaattcg
     6361 ccgatctgtt cgaggatcag tatgtccgcc agggggaata ccagaacagg acctttgagg
     6421 aaacgcttga actcggctgg aatcttctca ccatcatccc ggtgaaggaa ctgaagcgtg
     6481 tacgggatgc ctacattgag aagtacctcg caccgaggct gaaaaacggg gaaagggccg
     6541 aaacagtccc tgggaaagtg taagggagcc gtgtcatggc ccgattgaac gtaaacccca
     6601 acagaatgga gctttcaagg ctgaaaaaac gccttgtggt ggccaaacgg ggccacaagc
     6661 ttcttaaaga caagcaggac gcccttatca aggcatttct ggagcgtgcc cgggagggaa
     6721 aagtcctcag ggaagagctt gaaagcgagc ttgcggaatg ctacaagagc ttccttctgg
     6781 cccgcgccca gaccctgccc gccatgcttg agcaggcgct gatgatttcc ggtgcaaaat
     6841 gtgatctcca cgtcggcatg cagaacgtca tgagcgtgct ggtgcctgtt tattcagtag
     6901 agcagggagg atccccggtc aattacggcc tcgcaacatc cctcggcagc cttgatgtgg
     6961 cactcgaacg attcgcggcc ctcattccgc gtcttatcgc cctggcggca aaagaaaagg
     7021 cgatcaacct catggccgtg gaaatcgaaa agacccgcag gagggtaaat gcgctggaac
     7081 acgtgctgat accgggtttc gtggaaacaa tacgctacat tacaatgaag ctcgacgaac
     7141 aggagcggtc aacgctgagc cggctgatga aaatcaagga aattgtccgc tcccactgaa
     7201 agaagcagaa ggaggatccc gaacgggatc ctccttctgc ttctcagcag gttaaacagg
     7261 cttttgataa agaaatgctc cggcggattc aaaacctatc tttctgtact cgaaaatctg
     7321 ttctgtcgaa ccgacaagaa ggtatcctcc cggccggagt gcgttgaaaa atttgtgata
     7381 gagggcggtc ttggtttcag ctgaaaaata tatgaccacg tttctgcaga ggatgaggtc
     7441 aaagtcttcg gcaaaagtat cttcgataag attcagtctc tggaagttga ctcttccctt
     7501 aacctcctgg gtcaccgaat atgtttcccc tccgtcctct gtggtgaaat actttgaaac
     7561 aagctcaggc ggaacattaa cgatctgccg cttgaggtag gttccttttt tggctatgga
     7621 tatggccccc tggtcaatat cgctggcaag taccggggac tgggcagcaa gctttgcatc
     7681 aagggaaagg atggagaggg agtagggctc ctctcctgtc gcacttcccg cactccataa
     7741 tttgagttgt ttctttcccc tggcctggat caggcgggga ataatcgtct cccgaagctc
     7801 cgtccacttc gcggggttgc ggaaaaattc tgaaacgttg atggtaagat agtccagaaa
     7861 ttccctcagt ttctggtcat tttctttcat tgtcttgaaa tacaggtcgt attcagtaac
     7921 attccagcgt tgcatcaaca tgtgaactct cctgtggatc tggtttttat aagagttcaa
     7981 gtcgaggcca gtcagttttt tgacattcgc tttgaaaata tcgtattgag gcgagtcata
     8041 gcttgatttt tcaatcatcg ggggaatctc ttctccttta agatggtaca aaatgatata
     8101 gaggtatatt atactaaact cctccaaagt ttacccatga aacctttctg gtgatataat
     8161 tttcgcggga gtattctagg tgaccgctcc ctttcgggga gaggaaagtc cgggctccga
     8221 agggcaggac gctgggtaac acccagttgg tgcgaaccaa aggaaagtgc cacagaaaac
     8281 aaaccgcccc gcaggggcaa gggtgaaaag gtggggtaag agcccaccgg gtgaaaagtg
     8341 attttcacgc caggcaaacc ccgtccggag caagaccgaa taggggagga agacgttgcc
     8401 cgccgactcc cgggtacggt cgcgcaaagg ttgccgtgag gcgaccttca gataaatggt
     8461 caccctgtga cagaacccgg cttattgaat gctccctata ttctcaattt aaataggtct
     8521 tttggctcat attactcgtt tttttgaggg ccaaaaggcc tatttatttt tttgtggtgg
     8581 tggtagggtg ggagcaagtg ggggaaaatg tcgcaaagtg ggaggaagtc ccatgctggt
     8641 gggaacatac gaacacaaaa tcgactccaa ggcgcggatc gttcttccct cgaagttcag
     8701 ggaagagctt ggagagtgcg ttatttccac catcggcatc gacaggtgcg tttcccttta
     8761 ttcccgctca aggtgggaga atgtcatgac aaaactccag gatctcccct tttcaaaaag
     8821 caagacccgc ggactcctca gggtgatgct ttcaagcgca catgaactcc ccgttgactc
     8881 cgcaggaaga attctgcttc ctccggttct tcgccagcac ggcgaccttt cccaggacgt
     8941 cgtttttgtc ggagtgaacg atcacgtgga gctgtgggac cgcgacagat ggaatgaata
     9001 cagggaagag atggttgggg agctttccga aatagccgag ggagttgaag gattttgacg
     9061 gagcatatcc ccgttctcat cgctgaaatc ctggcctttt tacaaacccg cccgaaggtc
     9121 gggaaagtgc tcgattgcac ccttggtctc ggaggatatt cagaggccgt tcttgaaaca
     9181 tttccagaag ttcaagtgtg gggactcgat caggacgatg aggctatacg aattgcctcc
     9241 gacaggcttg agcctttcgg cgacagattt catccaatcc acggaaattt cgcggaagca
     9301 gctgaactgg cggggaatgc cggtcctttc gacgccattc ttttcgatct cggggtttca
     9361 aacctccaga tctctgaagg ataccgggga ttttctttcc agtcggacgg cccccttgat
     9421 atgagaatga acaggcattt cagcctgacg gctgaatatc ttgtgaacaa ctggactgaa
     9481 aaggaactct ccgacatttt ctggaaatat ggtgaggagc gctattccag gcagattgcc
     9541 agg
//
LOCUS       DAOTHD010000003         9012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_9226_length_9012_cov_2.914704, whole genome shotgun sequence.
ACCESSION   DAOTHD010000003 DAOTHD010000000
VERSION     DAOTHD010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 9012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9012
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9226_length_9012_cov_2.914704"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3793
                     /locus_tag="P5219_00110"
     CDS             <1..3793
                     /locus_tag="P5219_00110"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRX25186.1"
                     /translation="EGYSTLSTEWYSSVHPEAEVPEVKLYLSRDTQKKPGSARLAVLD
                     RMTGSPVAGVSVEVTSPQGFQQGVTDDSGLAAIGIPDGRVALRFTRKGYAPLETELFN
                     SYLDEAERVFYLDSDSAKAAFLVRDGITGNILEGVRVFRVEGEAGHDLGITGTGGILE
                     TSMPRGGFSFRFELDGYDPLITGFHISGNGKDDRSVEEVFLSPQKNPHSFRGIVKTPA
                     GEPLPGVSITMKQKETTISLQSLGDGTFALTPVPGPIDLTFTSAGYHELKTQTYFSRG
                     VLPPEEEEFFLFPSDSPYPDAPGRILFSAMDALTGEPLERFSAYLLDNGWAGYHNGTA
                     ATESRPGNRNTVIQCEGYYETGSFYPTVFPGKTVKKSIQLHPSTGKMQFRVLDALSGE
                     PVPAFSGYLLDNGWARYENGSAETEAKPGNRNTVIKAPGYNETGSFYPTVFPGRTVTR
                     TIFLNPSSGRIEFSVLDMVSGKPVPRFSAYLLDNGWAQYENGSAAAENQPGKRNTVIK
                     ASGYKETGSFYPAVFPGRTIRRTVYLAPSTGKLKLSVKDGLTGKPLPVFSAYLLDGGW
                     AQYRDGKGSSEAQPGNRNTVVKAPGYFETGSFYPTVFPGKTTERTLFLQPSLGRADFS
                     VADVLTGEPLPAFSAYLLDNGWARYENGKASAEAQPGNRNTVIKADGYFEAGFYPTAF
                     PGRSTTHTVYLHPREPLGQVNLKGIDLVSGETVNDVTFQPHGESPLRAPGGTALHSSR
                     THFRHQWFRASSPGYKGNDAPGFFTSGRRDVELAIPLERIVPPGKGNLLVTVADSEGK
                     TLSGAAVTLSCTGTAVRGKTNAAGQILFRNVPSGIHTLSASAKGYGKSSSRTAVEGGE
                     TEKTALKLSLLSELRPHAPFSPVIISVPSDEILRPGERKILRAVLENRGDEGGRTFCS
                     LEIPDMGRIVRETVLGPGERAEVPFEVLVPEDSVNSRVYALVKAGDQSKRGVLSIEAP
                     KFTLSARTGRETYLEGDVLDLEVEVTSDSGDGEYQVRASFNEETRLEAVKFSGGKGKV
                     LFRDIPVSFRGNKLFYGLYHVSGRSILLNALQVQDGSGDVHLVPEKQQYMAGETVRIR
                     ISGKAGEPLSVASLLLPGGIPGAEGVKEITPGSDGTAILDVPLPRAIATGSYPFRCGE
                     RSVNVDVRGTETKILDRRLEEENGENAGLLLFWKATSSGEIPCTWSVEAFPPDEEGYV
                     LAEGKITLKGEWGDYMIRVEPEDDEPCDFLLRLEADILSPSGIPLAEIRYSRAEREKQ
                     P"
     gene            3951..5174
                     /locus_tag="P5219_00115"
     CDS             3951..5174
                     /locus_tag="P5219_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRX25187.1"
                     /translation="MSGIWQDNFSSVAKNTRPSPIREMLAVIKQPGMISFAGGMPAPE
                     VFPVDQFYEGVHILKDQGKDLLQYGTTEGYPPLKEFLASWTAQRMGRKVGPDEMLITT
                     GSQQALDLFAWSMIDKGDYVITEDPSYLAALTVFFNHGGSFLGVPTDRDGMQVDLVPG
                     IIEKARSEGKKIKFIYTIVNFHNPGGSTMSVERRKKLVEISKQYNIPIFEDDPYGYVR
                     YEGQHLPSIFSFHDQGGVIYAGSFSKILSPGTRVGWVTGPKEIIRNLTVFKQGTDLCS
                     SPITQALVAEYCINGHLDRHLPVIIGNYRVKRDAMEKSFEKHLAPLGVTWVKPEGGFF
                     YWLKFPTVNTNDLFKKAIDKKVAFVPGDPFCPTAGTGIHNARLCYTFPSPEIIEEGVK
                     RLAEAVTELAIPAVH"
     gene            5303..6712
                     /gene="mnmE"
                     /locus_tag="P5219_00120"
     CDS             5303..6712
                     /gene="mnmE"
                     /locus_tag="P5219_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRX25188.1"
                     /translation="MDSRSPHSVTQDTIAAIATAWGEAGIAIVRLSGPEARRLADRQL
                     VLTVPLSGTPPRYLRNGFLLDEKGEAIDQVLAVWFAPPKSYTGEEVVEIHTHGGTLVA
                     QKCLELLLCRGARLAEPGEFTKRAFLSGRIDLTQAEAVLGIIRSRSDEALRAAARTLK
                     GELASFARDIYDELLALSGKMEVSLDFPEEDLPYGNDEDTGHSLLAIRQSLEDLLDRC
                     ATGFLLREGIRVAIVGRPNVGKSSLLNALLRESRAIVTAIPGTTRDLIEEVLTYRGIP
                     LRLVDTAGLGDPSDEVEAIGISLAEEAFKNADVRIWVADGSTPPAPFDFDMAERLRDL
                     VHVVAVNKSDLPPGREDTGRTTEEAISELLPESPVLSISAKKGTGLDNLKDAVVSSIA
                     GGGALDSGLNASARQVNEIRAAIGFLRDASEALESGLGQDIAGSGLAEARSAMERLLG
                     ITGDDTLLDYIFSQFCVGK"
     gene            complement(6755..8218)
                     /locus_tag="P5219_00125"
     CDS             complement(6755..8218)
                     /locus_tag="P5219_00125"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HRX25189.1"
                     /translation="MKITASPLTGGIVSGSGAVALYVFSGESLPEGILSPGNEAAAAA
                     LLEDPSFKAKKGKIAKVTLPGSPLSRIYLVGLGERKDLGEDSLRSRTAELVRRAKGDG
                     ISRISAVLSVLPDRNASCAVGEGAELGSYSFEKYKTRKDEDRLPEPGEFVLYAGDDDG
                     LSLGKMLACTQNMARDLANEPGNRSNPQTLAEYALAEARDFGLEVEVWDERKILEEGM
                     EGLYSVGMGSSTPPRLVRLSWKPEGAPLRKVVFVGKGITFDSGGLNLKPGDFIRSMKS
                     DKSGACNVFALLRAAAKMKLPVEVHGIVALAENMPGGKAFRPDDILRMRNGKTVEIDN
                     TDAEGRLVLADALSYASELRPDAIVDVATLTGACAVALGTSIAGLFTPDESLAGSLLE
                     AGKTRGERLWRLPDDDERIAESMKSPVADLVNSGSRYGGAIFAARFLREFVGEGISWA
                     HLDIAGVDFNKDEYSVYSKGASGFGVRTCLQYLLSLR"
     gene            complement(8241..>9012)
                     /locus_tag="P5219_00130"
     CDS             complement(8241..>9012)
                     /locus_tag="P5219_00130"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRX25190.1"
                     /translation="VAALLLFLLLRGRMRFLPLREAAVFAGLGFLGVFFHNALQAYAL
                     RTVSAGMSGLIVAANPIAIALLGALILGERLDGRKKGGILLAAFGVLVIISKGDPGVF
                     LRRGYSPGELLMVVSVFSWGLFSVFSRKALRKTSPELGMAYALSFGWLFATLPFFFSG
                     GALEIPSISAGAWANILFLGVCCSALAYLFWYDALRELPASEAGVFLYINPVVAVLLS
                     AAFLGENVTLSMIAGGVLVFTGVWFVNTKKRPSPAGEK"
BASE COUNT         2038 a         2683 c         2556 g         1735 t
ORIGIN      
        1 tgaaggatac agcaccctgt caacggaatg gtactcgtcg gtccaccctg aggcggaggt
       61 gcccgaggtg aaactgtatc tttcccggga cacgcaaaaa aaaccgggaa gcgcacgact
      121 ggccgtcctc gacaggatga cgggcagccc cgttgcagga gtttccgttg aagtgacctc
      181 accccaggga ttccagcagg gcgtcacgga cgacagtggc ctggctgcca tcgggattcc
      241 cgacgggaga gtcgctctca gattcacacg gaagggatac gctccccttg aaacggaact
      301 cttcaacagc tacctggatg aggctgaaag ggtgttttac ctcgactccg attccgcgaa
      361 agcagcgttt ctcgtgaggg acggcattac gggaaacatc ctggagggag ttcgggtatt
      421 cagggttgag ggggaggcgg gacatgatct tggaataaca ggaacgggag gaattctcga
      481 gacgtccatg cccagaggcg ggttctcctt caggtttgag ctggacgggt acgatcctct
      541 catcacaggc ttccatattt cagggaacgg caaggacgac aggtctgtgg aagaggtctt
      601 cctctccccg caaaaaaatc cccattcctt ccggggaata gtgaaaaccc ctgcgggaga
      661 acctcttccc ggcgtttcca ttaccatgaa gcaaaaagaa acaaccattt cactccagag
      721 tctcggggac ggaacttttg ccctgacacc ggtccctggc cccatcgatc ttacgtttac
      781 ttctgccggg taccatgaac tcaagaccca gacgtatttc tccaggggcg tgctgccgcc
      841 ggaggaggaa gaattcttcc tcttcccctc cgacagtccc tatcccgatg cccccggcag
      901 gatactgttc agcgccatgg acgcccttac cggagagcct ctggaaagat tcagcgccta
      961 tcttctcgac aacgggtggg ccggatatca caatgggacg gcagccaccg aatccaggcc
     1021 cggtaacagg aatacggtca tacaatgcga aggctactat gaaaccggtt ccttttatcc
     1081 aaccgtcttc cctgggaaaa cggtgaaaaa atcgatccag cttcacccgt ccacgggaaa
     1141 aatgcagttc cgagtactag acgccctctc cggggaaccc gtcccggcgt tttcgggcta
     1201 tcttctcgac aacggatggg cccggtacga aaacggttcc gcagaaacgg aggccaagcc
     1261 cggaaacagg aacacggtca tcaaggcccc gggatacaac gaaacagggt ccttctaccc
     1321 cacggtcttt cctggaagaa cggtgacgag aaccattttt ctcaatccct cgtcgggccg
     1381 catagaattt tctgttctcg acatggtctc cggaaagccg gttccccgat tctcagccta
     1441 ccttcttgac aacggatggg cccagtacga aaacggttcc gcggctgcgg aaaatcagcc
     1501 ggggaagaga aataccgtta tcaaggcatc cggatacaaa gaaaccggct ccttctatcc
     1561 tgccgttttt ccgggaagga ccatcaggcg cactgtttac cttgccccct ccaccggaaa
     1621 actcaaatta tctgttaaag acggtctgac cggaaagcca cttcccgttt tttccgccta
     1681 cctgctggac ggaggatggg cccagtaccg ggacgggaag ggctcctcgg aggcccagcc
     1741 ggggaacagg aatacggtcg tcaaggcacc gggatacttc gaaacggggt cattctatcc
     1801 caccgttttc cccggaaaaa ctacggagcg cactcttttt ctccagcctt cactgggacg
     1861 ggctgatttt tctgtggcgg acgtcctcac gggagaaccc ctgccggcct tttccgcgta
     1921 tcttctcgac aacgggtggg cacggtacga aaacggaaaa gcctccgcag aagcccagcc
     1981 gggaaacagg aacacggtga tcaaggcgga tggctacttc gaagcagggt tttaccccac
     2041 ggcttttcct ggccgctcaa ccacgcacac cgtgtatctt catcccagag aacctcttgg
     2101 ccaggtgaac ctgaaaggaa tagacctggt ttcaggggaa acagtgaacg acgtcacctt
     2161 ccagccccat ggagaatctc ctctcagagc tcccggaggc acagccctcc attcatcccg
     2221 gactcatttc agacaccaat ggttccgggc ctcttccccc ggatataaag gcaatgacgc
     2281 ccccggcttt ttcacctcgg gccgcaggga cgtcgaactg gccatccctc tggaacggat
     2341 cgtcccgccg ggaaaaggga atcttctcgt aacggtggct gattccgaag gaaaaaccct
     2401 ttccggtgcg gctgttaccc tctcctgtac cgggacggca gtccgcggga aaaccaacgc
     2461 ggcgggacag atcctcttca ggaacgtgcc gtcgggaata cataccctct ccgcgtcggc
     2521 aaaaggatac gggaagagca gttcgcgaac cgccgtggaa ggcggggaaa cggaaaagac
     2581 cgcgctgaag ctgtcccttc tgtcggaact ccggccccat gctcctttct ctcccgtcat
     2641 catcagcgtt ccttcggacg agatcctgag gccgggagaa agaaaaatcc tccgggccgt
     2701 tctggaaaac aggggagatg agggcgggag gactttctgt tccctcgaga tccccgatat
     2761 gggaagaatc gtcagggaaa cagtgcttgg accgggcgaa cgggcggaag ttcctttcga
     2821 agtcctggtt ccggaagaca gtgtcaattc cagggtgtac gcccttgtga aggccgggga
     2881 ccagtcaaaa cggggggtgc tctccatcga ggcacccaaa ttcactctct ccgcgcgaac
     2941 gggcagggaa acctacctgg agggtgacgt tctggatctt gaagtggaag tgacctccga
     3001 ctcgggagat ggggaatacc aggtccgggc ttccttcaac gaggagaccc gcctggaagc
     3061 ggtaaaattc tccggaggaa aaggaaaggt tctgttccgg gacatacctg tctctttcag
     3121 ggggaacaag ctcttctacg gcctttacca tgtctcggga cggtccatat tgctcaatgc
     3181 actccaggtc caggacgggt caggagacgt ccatcttgtt ccggaaaaac agcagtacat
     3241 ggcgggtgaa actgtacgga tccggatctc cggaaaagcg ggagaacccc tttcggtcgc
     3301 ctccctgctc ctccccggcg gaataccagg cgctgaggga gtgaaggaaa taaccccggg
     3361 atccgacggt acggcgatac ttgatgttcc ccttccccga gccatagcga cgggatccta
     3421 ccccttcagg tgcggtgaac gttccgtgaa tgttgacgtc cgggggacgg aaacgaaaat
     3481 actggacagg cgcctggaag aagaaaacgg ggaaaatgcc gggctgcttt tattctggaa
     3541 agcgacctcg agcggggaga tcccgtgcac atggtcagtg gaggcgttcc ctccggatga
     3601 agaagggtat gtgcttgcgg aagggaagat cactctgaaa ggagaatggg gcgactacat
     3661 gatccgggta gaaccggaag acgacgaacc ctgtgatttt ctgctccgcc tggaagccga
     3721 tattctttca ccctcaggga tacctctcgc agaaatacgg tattccaggg cagaaaggga
     3781 aaagcaacca tagccggccc tgcatccggc ccataatttc cgcggggaac ggcataaagc
     3841 cgtttcccgt tttcgtgcta taatatacct gtattgtaga tttttttgct tctatggcgt
     3901 aaaatccgaa ttattagtga aaacacgaac aaatcaaggg aggcgaagaa atgagcggga
     3961 tttggcagga caacttcagc tccgtagcga agaacacccg gccgtcgccg atccgggaaa
     4021 tgctggcagt gatcaagcag cccggcatga tctccttcgc ggggggcatg cctgcccccg
     4081 aggttttccc ggtcgaccag ttctatgaag gagttcatat cctcaaagac caggggaagg
     4141 atcttctcca gtacggaact accgagggat accctcctct caaggaattc ctcgcatcgt
     4201 ggacggcaca gcgaatgggg cgcaaggtcg gaccggacga aatgctcatc actaccggat
     4261 cgcagcaggc ccttgatctc ttcgcatggt ccatgatcga caagggggat tatgtcatta
     4321 ctgaagaccc ctcctacctt gcggctctca cggtgttctt caaccacggc ggcagctttc
     4381 tcggcgtccc cactgaccgg gacggtatgc aggttgacct tgtccccggc ataatcgaga
     4441 aagccaggtc cgaaggaaaa aagatcaagt tcatctacac catcgtgaac ttccacaatc
     4501 ccggcggctc caccatgtcc gtggagcggc gcaaaaagct ggtggagata tccaaacaat
     4561 acaatatccc cattttcgag gacgaccctt acggatacgt caggtacgaa ggccaacacc
     4621 tgccgtccat attctccttc catgaccagg gcggggtgat ctatgccggg tccttctcga
     4681 aaatcctctc ccccggaacc cgcgtaggct gggtaaccgg cccgaaggag atcatccgga
     4741 acctcaccgt gttcaaacag ggaacggacc tctgctccag cccaatcacc caggcacttg
     4801 tggcggagta ctgcatcaac ggccacctcg accgtcacct tcccgtgatc atcggcaact
     4861 acagggtgaa gcgggacgct atggagaaga gcttcgaaaa acacctcgca ccccttggag
     4921 tcacgtgggt caagcccgaa ggaggcttct tctactggct gaagttcccc acggtaaaca
     4981 ccaacgacct ttttaaaaag gccatcgaca agaaggtggc cttcgttccc ggtgatccct
     5041 tctgccccac tgccggcacg gggatccaca atgccagact gtgctacacc ttcccgtccc
     5101 cggaaatcat cgaagagggc gtgaagcgtc tcgccgaagc agtcaccgaa ctcgccattc
     5161 cggctgttca ctgaaaacac atttttttag gagcctctgc tccctccccg gcggggaggg
     5221 agtttttttc tgcacccggg ggcgatttga tgtaagatac ggaacaaggc gagaagccgg
     5281 aaaatcaggg gaggagagag cgatggattc caggagtccg cacagcgtta cacaagatac
     5341 catagcggcc attgccacgg cgtggggaga agccggcatt gccattgtcc gcctgtcagg
     5401 cccagaagcg cgcaggctgg cggacaggca gcttgtcctc accgtccccc tttcgggaac
     5461 tccgccgcgg tacctccgga acggctttct tctcgacgaa aaaggagagg ccatcgacca
     5521 ggtgcttgcc gtttggttcg ccccccccaa aagctacacg ggggaggaag tggtggaaat
     5581 tcacacccac ggcggaaccc tggtggcaca aaaatgcctg gaacttctcc tctgccgggg
     5641 agcccgcctc gccgagcccg gtgagttcac gaaaagggct tttctctccg ggcgaatcga
     5701 cctgacccag gcggaggccg tcctgggaat tatccgttca cgaagcgacg aggctctcag
     5761 ggctgccgcc cggacgctca agggagaact cgcttccttc gcgagggaca tttacgacga
     5821 actgctggct ctttcaggaa aaatggaagt ttctctcgac tttccggagg aagacctgcc
     5881 ctacgggaac gacgaggata cgggccattc ccttctggca atacgccaga gcctggagga
     5941 cctgctggac cgctgcgcca cgggatttct tctccgggag gggatcaggg tggccatcgt
     6001 gggcaggccg aacgtgggta aatcttccct tctcaacgcc ctcctcaggg aatcgcgggc
     6061 aatcgtcacg gcgattcccg gcaccaccag ggacctcatt gaggaagtac tcacctaccg
     6121 gggaattccc ctccggctgg tggacacggc ggggttgggg gatccctccg acgaggtgga
     6181 ggccatcggt atctccctgg ccgaggaggc cttcaaaaat gccgacgtgc gcatctgggt
     6241 ggccgacgga agcactcctc cggcgccctt cgattttgac atggcggagc ggctgcggga
     6301 tctcgtccac gtggtggcag tgaacaaatc agaccttcct cccggaaggg aggataccgg
     6361 aagaaccacc gaggaggcta tctccgaact tctgcccgag agccctgtgc tatcgatttc
     6421 ggcgaagaaa ggcaccggac tggataatct gaaggatgcc gtggtctcct ccatcgcagg
     6481 cggcggggct ctggattcgg ggctgaacgc ctccgccagg caggtgaacg agattcgtgc
     6541 agccatcggt tttctcaggg atgcctccga agccctggaa tccgggcttg ggcaggacat
     6601 cgcaggatcc ggccttgcag aggcccggtc agccatggag cgcctcctgg gcattaccgg
     6661 agacgacacc ctgctcgact acattttcag ccagttctgc gtcgggaaat aatccgatgg
     6721 gaggagcccc gaggctcctc cccaccccgg aatactagcg gagagacaaa agatactgca
     6781 gacatgtcct gacgccgaac ccgctggccc ccttgctgta gaccgaatac tcatccttgt
     6841 tgaagtccac cccggcaatg tcgagatggg cccaggagat cccttccccg acgaattcac
     6901 gaagaaaccg tgccgcaaag atggccccgc cgtacctgct ccctgaattc accaggtccg
     6961 caacagggga cttcatggat tccgcgattc tctcatcatc gtcgggaagg cgccacagcc
     7021 tttctcccct tgtctttccg gcttcgagaa gactcccggc caggctttcg tcgggtgtga
     7081 agagccctgc aatggacgtt cccagggcga cagcgcaggc cccggtcagg gtggccacgt
     7141 cgacgatggc atccgggcga agttccgagg cgtagctcag ggcgtcggcg aggacgagcc
     7201 gcccctcggc gtctgtgttg tcgatttcca ctgttttgcc gttcctcatc cggagaatgt
     7261 cgtcagggcg gaacgccttt ccgcccggca tattctccgc aagggccacg attccgtgaa
     7321 cctccactgg aagcttcatc ttcgccgccg cacggaggag tgcaaaaaca ttgcatgccc
     7381 cggatttatc gctcttcatg gaccggatga agtcccccgg tttgaggttc agccccccgc
     7441 tgtcaaaggt gatgcccttc ccgacgaaga ccactttccg caagggggca ccctcgggtt
     7501 tccaggaaag cctgaccagc ctcggaggtg tggacgaccc cattcccacg gaatagagac
     7561 cctccatccc ctcttcaaga atttttcttt cgtcccaaac ttccacctcg aggccgaaat
     7621 cacgggcttc tgccagggcg tactccgcca gcgtctgcgg attggacctg ttccccggct
     7681 cgttcgcaag atcccgtgcc atgttctggg tgcaggcgag cattttcccc agggaaagcc
     7741 cgtcgtcgtc tccggcgtac aggacgaatt caccgggttc gggaagcctg tcctcatcct
     7801 tccgggtctt gtatttttca aaggaatagc ttccgagctc agctccctcc cccaccgcac
     7861 agcttgcgtt ccggtccgga agaaccgaga gaacggccga aatacgggag atcccgtctc
     7921 ccttcgctcg ccggactagc tccgcggtcc tggagcggag gctgtcttcc cccaaatcct
     7981 tcctctcgcc gaggccgaca aggtagatgc gggaaagggg agaacccgga agggtcacct
     8041 tcgctatttt tccttttttt gccttgaacg agggatcctc gagaagggca gccgcagccg
     8101 cttcgttccc gggtgaaaga atcccttccg ggagagattc tcccgaaaat acatacagag
     8161 ccactgcccc gctcccggac acgattccgc cggtaagagg ggatgcagtt atcttcattg
     8221 aatccacgct ccctgaatat ttatttttcc ccggcagggg atggtctttt tttcgtgttg
     8281 acgaaccaga ctcctgtgaa gacgaggaca cctccggcga tcatggagag agtgacgttt
     8341 tcgccgagaa aagccgccga aagaagcacg gccaccacgg gattgatgta gaggaaaact
     8401 cccgcttccg aagcgggaag ctcccggagg gcatcgtacc agaaaaggta ggccagggcg
     8461 gagcagcata ccccgaggaa caggatattg gcccaagccc cggcggaaat ggatggaatc
     8521 tccaaagccc ctcccgaaaa gaaaaaggga agggtagcga acagccatcc gaaagaaagg
     8581 gcgtaagcca tcccgagttc cggagaggtt ttccggaggg ccttcctgga aaatacggaa
     8641 aaaagccccc atgaaaagac gctgaccacc atgagcagct cccccgggct gtagcctctc
     8701 ctcaggaata cgccggggtc gcctttcgag ataatgacca ggacaccgaa ggccgcgaga
     8761 aggataccgc ctttctttct tccgtcgagc ctctcgccga gaataagggc ccccaggagg
     8821 gcaatggcga tggggttcgc cgccacaatg agccccgaca tgcccgcaga cacggtccgc
     8881 agggcatagg cctggagggc gttgtggaaa aagaccccga gaaagcccag ccccgcgaag
     8941 acggccgctt ccctcagggg aagaaaccgc atccttcccc tgagaagaag aaacaggagc
     9001 agggccgcca cc
//
LOCUS       DAOTHD010000004         8822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_9492_length_8822_cov_3.302954, whole genome shotgun sequence.
ACCESSION   DAOTHD010000004 DAOTHD010000000
VERSION     DAOTHD010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 8822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8822
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9492_length_8822_cov_3.302954"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..1133)
                     /gene="pheS"
                     /locus_tag="P5219_00135"
     CDS             complement(117..1133)
                     /gene="pheS"
                     /locus_tag="P5219_00135"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRX25191.1"
                     /translation="MEQDFAGQLSRAATSGELQQVKVAFLGKKGILTSFLKKLGSLPT
                     GERPAAGEAVNSLRDRIESSLAEKRTALEEAESAEEERRNRIDVTLPPRGREWGGIHP
                     VAQLTQDVVEILAGLGFSVALGPEIEDDFHNFEALNIPASHPARDMQDTFYFPEGKLL
                     RTHTSPVQVRSMLKYGAPIRIVCPGKVYRRDSDPTHSPMFNQLEGLLVEKDISVADMK
                     GCLEALMAAIFARPLKARYRASYFPFTEPSLELDIECVECSGRNPSCRICKGTGWLEV
                     AGLGMVHPNVLRYGGIDPEVYNGFAWGIGLDRIAMLKYRLTDLRVLFEGNVPYLLSGR
                     TLSC"
     gene            complement(1160..1840)
                     /locus_tag="P5219_00140"
     CDS             complement(1160..1840)
                     /locus_tag="P5219_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRX25192.1"
                     /translation="MDSKQTILVVGLGRFGVSVCEKLAELGQHVIAVDIDKQRVEEVA
                     DVVEYCAQLDATDEDLLVKVGAREADIAVVAIGNNIEASILATAILKGLNIPKVIARA
                     QTSIHGRVLSRVGAHKVVFPERDAGKNIAETLVNPWLSAFSAVPGGGVLVGEIEASAG
                     MVGKSLMELEFRKTYNCIVLLFDRKGSRFLPRPDSVIQEGDKLLVAGTGEDIAEMMEK
                     KDKEENGK"
     gene            complement(1855..3174)
                     /locus_tag="P5219_00145"
     CDS             complement(1855..3174)
                     /locus_tag="P5219_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRX25193.1"
                     /translation="MKTYSSLRIEKTITLGFLSLIAAGTLLLWAFNSGHEKPLSFLDA
                     LFTSTSAVCVTGLVVADTGRDLAPASQWVVLVLIQLGGLGVMTAATAIMVLLGWRVGI
                     RQRLLFSGGFGVETPSGAVRLLYRMLGFTLLVEGMASLPLFFVFLRSFPPGEALFNAL
                     FHSVSAFCNAGFSTLRGGLEGYSGQFLLPGVIMVLIVTGGLGFLVIGDIFVRLRERSR
                     LSAHSVLVLRTTAVLIAAGTVLLFASDALHGLSGHPLHLKVWNALFQSVTARTAGFNT
                     ISMPLFSSLGLFVLCILMIIGASPGSTGGGIKTTTAGLLFYSSLSEIRGTGMAIGNRK
                     ISDGNVRRALAVTVLYILTVLLGIILLCLIEPFSFGALAFEAVSAMGTVGLSLGVTPS
                     LSAPGKVIIILLMFWGRVGILTFMYGMISRDRKSSKITFAETNIPVG"
     gene            complement(3205..3564)
                     /gene="rplT"
                     /locus_tag="P5219_00150"
     CDS             complement(3205..3564)
                     /gene="rplT"
                     /locus_tag="P5219_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRX25194.1"
                     /translation="MPRVKGGSASDRKRKKLFSITKGYWGRKKNVYRRAREAYLHSLS
                     RAYADRKRKKRDFRRLWITRINAASRANGLSYSVLMNGLKKAGISINRKMLSELAIND
                     MPAFVKLTEQARSALGK"
     gene            complement(3611..3808)
                     /gene="rpmI"
                     /locus_tag="P5219_00155"
     CDS             complement(3611..3808)
                     /gene="rpmI"
                     /locus_tag="P5219_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRX25195.1"
                     /translation="MPKLKSHSGAKKRFSFTGTGKLSYRKAGRGHLLTCKNPSRLRKL
                     RKTGYVDDTQMEYMRKLLPYS"
     gene            complement(3906..4550)
                     /gene="infC"
                     /locus_tag="P5219_00160"
     CDS             complement(3906..4550)
                     /gene="infC"
                     /locus_tag="P5219_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRX25196.1"
                     /translation="MVARKEDGDPRVNREITSREVLLIDENGVKMGVIPTVEGIRLAE
                     ERQLDLVEVAPQANPPVCRILDYGKYRYQLQKKEKDARKKQKVQALKEMKMRPKIDEH
                     DYDFKVRAIKGFLEDGHRVKVSVFFRGREMSFLNKGEEVLARVIADCDGLGKSEGNPR
                     MEGRYMRIMLTPLPPGQGKVAPKPEKKETARTTEQKNNGKPKETPTVSVEKPAE"
     gene            complement(4780..6699)
                     /locus_tag="P5219_00165"
     CDS             complement(4780..6699)
                     /locus_tag="P5219_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HRX25197.1"
                     /translation="MKKLLSAILPGESIPTRKLNGTAGRAALVLTVSTALVHFWMNSI
                     GLLIAIKMNAIHLGTLMAIIFLFYPAFAGSPRERPSMPDWVLAGISLGCMVWLLITYD
                     RLLQTNLQATFADLAVAVVTMALLVEASRRAVGLPLTVLSLLFLAYTRLGPYFPGLFA
                     HRGFNWERIIIRMALTDQGIYGVTLMVSSSYVFMFILFGAFLAASRTSEFFNDFSLAL
                     AGRYRGGPAKVAVVASALMGSISGSAQANVATTGAFTIPLMKRVGYMPHFAGAVEAAA
                     STGGILMPPIMGASAFIMSTFLGIPYVKIMIAGFTPALLYYLAIMFMVDLRAKKRGLK
                     GMEPGDIPSLKATMLDKGHMTIPLVVIIYLLVAGYTPLYSAFLGLFAIVLVSSLKRST
                     RMGIREVIEALDSGTRSAAPVGISCAIVGFIVGAVGMTGLGQVIAMNVIMFAGGKLWA
                     ALILCMIAAIILGMGLPATPCYIITATIAAPALQQMGVHPLAAHFFAFYYGTMSAVVP
                     PVALTSYTAAGLAGARPFKVAVSALGLALSGLLLPYLFVYNPDLLFIDFVLPKYLLDV
                     VTAIIGVFSLSCGIIGMFKRDMSFLERALFILAGILMVNPLRILRISSFVFFAALVAR
                     HWFSTEGAGRGEPSI"
     gene            complement(6785..7780)
                     /locus_tag="P5219_00170"
     CDS             complement(6785..7780)
                     /locus_tag="P5219_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRX25198.1"
                     /translation="MKSTKKILVLFCLALFLLSAAGSALASGKFVTIVTGSTGGTYYP
                     IGTILANHFNAALMDKGYKWSAQSSGGTVENLDMMQRSEAEMAIAMANLTGFAYTGTV
                     RYEGKKIENLRYVMGLWPDVTQFVVSGSSGIKTWADLKGKKVAVGPAASGTEFSSRVL
                     LKALAGLTFDDIRAEYVGYSEASQALQNGQLDAFNAEAGVPVAAVAELYAGRQEVNML
                     EFSPDDIAKLKKEAPFYAAVLIPAGTYPNQEKELRVAGIKSALLVGKDVPEELVYDML
                     KIVYSKKEDLKKEHAAFTKVDFDNPVDGLFGAPLHPGAVKFFREIGMTIPQELLP"
     gene            complement(8021..>8822)
                     /locus_tag="P5219_00175"
     CDS             complement(8021..>8822)
                     /locus_tag="P5219_00175"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRX25199.1"
                     /translation="GLMRVRCFTQDDAHLYCRPDQVKDEIIGIMKLCDYIYRDVFGFR
                     YTMELSTRPENSMGSEEQWAIAENALKQALEETGSEYRLNAGDGAFYGPKIDFHLEDC
                     IGRTWQCGTIQLDFQMPEKFDLTYVGADGKEHRPVMLHRTVLGSLERFFGILVENFAG
                     SFPYWIAPVQVRILPVSGEFADYAGTVAAELRASGIRVEVDERDEKLGKKIRDAQTQK
                     IPYMVVVGEKERESGGVAPRERSRGDLGSMSMKQFREVLAEEFNPLKS"
BASE COUNT         1947 a         2498 c         2456 g         1921 t
ORIGIN      
        1 catggcacag ggcctttcca ccgattccac ctcgcaccct gtgagggtga gccgttcggc
       61 cacttcttcc agggtggcgg gaatggagag cgtttcattg agcatgttcc aggaaatcag
      121 catgagaggg tcctccccga gagaagatag ggcacgttgc cttcaaagag gacgcgcagg
      181 tcggtgagcc tgtatttcag catggcgatg cggtcaaggc cgatgcccca cgcaaagccg
      241 ttgtagactt cagggtcgat tcccccgtac cggagcacat tgggatgcac catgccgagt
      301 cctgccactt ccagccaacc cgttcccttg cagatcctgc aggaaggatt cctgcccgag
      361 cattccacgc attcgatatc cagctccagt gaaggctccg tgaaggggaa atagcttgcc
      421 ctgtaccggg ccttcagggg acgggcaaaa atggcggcca taagggcctc aaggcagccc
      481 ttcatgtcgg ccacggagat gtccttctcc accagcaggc cttcgagctg gttgaacatg
      541 ggggaatggg ttgggtcgct gtccctgcgg tatacctttc cggggcagac aatacggatg
      601 ggtgccccgt atttcagcat ggacctgacc tgcacgggtg aggtgtgggt cctgagcagc
      661 ttcccttcag ggaaatagaa ggtatcctgc atgtccctcg ccggatggga cgcaggaatg
      721 ttcagggcct cgaaattgtg gaagtcgtct tcgatctccg gtccgagtgc gacggaaaaa
      781 cccaggcccg cgaggatttc caccacgtcc tgggttaact gggccacggg atgaatcccg
      841 ccccattccc tgccccgggg cgggagggtg acgtcgatac ggttccgccg ctcttcttcg
      901 gccgactcgg cttcctcaag ggctgtcctt ttttctgcca gggacgactc tatccggtca
      961 cggagagaat tcaccgcctc gccggcggca ggacgctccc cggtagggag cgatccgagt
     1021 tttttcagga aggaggtcag gattcccttt tttccgagaa aggccacctt cacctgctgc
     1081 agctccccgg atgttgccgc ccgcgagagc tgtccggcga agtcttgttc tattgtatga
     1141 agttcgcttg ggattgatgt cacttgccgt tttcctcctt gtcctttttc tccatcattt
     1201 ctgcgatgtc ctctcccgtt ccggcgacaa gaagcttgtc cccttcctgg atgacgctgt
     1261 ccggccgggg caggaaccgg cttcccttcc ggtcgaaaag gaggacaatg caattatacg
     1321 tcttccgaaa ttcgagttcc atcagcgatt ttcccaccat cccggccgat gcttcaatct
     1381 caccgacaag aacgcctccg cccggaacgg cggaaaaggc agaaagccat gggttgacca
     1441 gcgtttccgc aatattcttg cctgcatccc tttccggaaa gaccacttta tgggctccca
     1501 cccgcgaaag tacccttccg tgaattgaag tctgggccct cgcgatgact ttcgggatgt
     1561 tcaacccctt caggatggcc gtggcaagta tgcttgcctc gatgttgttt ccgattgcca
     1621 cgacagcgat atccgcctct cgcgcgccaa ccttcacaag gaggtcttca tcggtggcgt
     1681 cgagctgggc acagtactcc acaacgtccg caacttcctc gactctttgt ttgtcaatgt
     1741 ctacagcgat aacatgctgg ccgagctccg caagtttttc gcagacggag acaccgaagc
     1801 gcccgagtcc cactacaagg atggtctgtt tgctgtccat ggtccgcctc cctgctatcc
     1861 cacgggaata ttcgtctcgg cgaaggtgat tttcgacgat ttccggtccc gggagatcat
     1921 cccgtacatg aatgtaagaa taccaacccg cccccagaac atcaacagaa tgatgatcac
     1981 ctttcccgga gctgaaaggg acggtgtaac acccagcgaa aggcccacgg ttcccatggc
     2041 tgaaacagcc tcgaaagcca gagccccgaa agaaaagggt tcaatgagac acaaaaggat
     2101 aatgccgagg agaactgtga gaatgtacag cacggtgacg gcaagagcac gtctgacatt
     2161 gccgtcggag atcttcctgt tccctatggc catacctgtt ccccgtatct ctgaaaggga
     2221 ggaataaaag agcagtcccg ccgtggttgt tttaattccc cctccagtgg atcccgggga
     2281 tgctcctatg atcatgagaa tgcagagtac gaaaagcccg agggaggaaa acagcggcat
     2341 ggaaattgta ttgaatccgg ctgtccgtgc tgtgacgctc tggaaaaggg cattccagac
     2401 cttcaggtgg agaggatggc ccgaaagccc gtgcagtgcg tctgaggcga agagaagaac
     2461 agtacccgcc gcaatcagga cggccgtcgt cctgagaacg agaacggaat gggccgacag
     2521 gcgggaccgt tcccggaggc ggacgaaaat gtcgccgatg accaggaagc caagacctcc
     2581 agttacgatg agaaccatga tcaccccggg aagaaggaac tgccccgaat acccctccag
     2641 cccgccccgc agggtggaaa acccggcgtt gcagaaggcg ctgaccgaat ggaagagggc
     2701 gttgaaaagg gcttctcccg ggggaaaaga ccggagaaat acgaaaaaca ggggaagaga
     2761 ggccatcccc tccacgagaa gggtaaagcc aagcattctg tacagcagcc tgacggcacc
     2821 cgagggggtc tcaactccaa aacctccgga aaagagaagt ctctgccgta tgccgacccg
     2881 ccatccgaga aggaccatga tcgcggtggc cgccgtcatg acgccgagcc caccgagctg
     2941 gataaggaca agaacaaccc attgtgatgc gggagcaaga tccctgcccg tatccgccac
     3001 cacaagcccc gtaacgcaca cggcagatgt gcttgtgaaa agagcgtcga gaaaagaaag
     3061 aggcttttcg tgcccggagt tgaaagccca aagaagcagg gttcccgccg caatcagcga
     3121 caggaacccc agggtaatgg tcttttcaat ccggagcgag ctgtaggtct tcactttcag
     3181 ccgggccgga accgccgatg gcggctattt gcccagggcg ctccgcgcct gctcggtaag
     3241 cttgacgaag gcaggcatgt cgttgatggc cagctcggaa agcatcttcc ggttgatgga
     3301 gattcccgcc tttttcagtc cgttcatcag cacgctgtaa gacagtccat tggcccggga
     3361 cgccgcattg atacgggtta tccacagccg cctgaaatcc cttttcttgc gtttcctgtc
     3421 ggcataggct ctggagagag aatgaaggta tgcttccctg gctcttctgt acacattctt
     3481 tttccgtccc cagtatccct tggtgatgga aaacagtttc tttctttttc tatcgctggc
     3541 gctaccacct ttgacgcggg gcatcgctcg tcacctcttt catgaatatt gactaaaaac
     3601 gaaatatacc tcagctgtac ggaagcagct ttctcatgta ctccatctgc gtatcgtcaa
     3661 cgtagccggt cttgcggagc tttctgagtc tcgaagggtt cttgcaggtg agcagatgtc
     3721 ctcttcctgc cttgcggtag gagagctttc ccgtccctgt gaaagaaaat cttttttttg
     3781 ctcccgaatg ggatttaagc ttgggcatga tgtgttcctc cttccccgga tattgagtca
     3841 agataaaagc ccgccgctgt ctgcggcagg cggaaaagcg gatcaaaatc tatgcagtca
     3901 cggaatcact ctgccggttt ttccacggat accgtcggcg tctctttcgg ctttccgtta
     3961 ttcttttgtt cggtcgtcct ggctgtttct ttcttttcgg gcttcggagc aacctttccc
     4021 tggccgggag gaaggggggt aagcatgatc ctcatgtacc ggccctccat gcgggggttc
     4081 ccttcgctct ttccgagccc gtcgcagtcg gcaatcacac gggcgaggac ttcttcgccc
     4141 ttgttgagaa aggacatctc gcggccccgg aagaacacgg aaaccttgac tctgtgtccg
     4201 tcctcaagga agcccttgat cgccctgacc ttgaaatcat agtcgtgttc atcgattttc
     4261 gggcgcattt tcatttcttt gagggcctgg actttctgtt tctttctcgc gtccttctcc
     4321 tttttctgga gctggtagcg atacttcccg tagtccagta tcctgcacac cggcggattg
     4381 gcctgagggg caacctcgac aaggtcgagc tgccgttctt ctgccagccg aattccctcc
     4441 accgtgggga ttacacccat cttgacgccg ttctcgtcga taaggagcac ttcacgggac
     4501 gtgatttcac ggttgactct gggatctccg tcttcttttc ttgctactat agcctttcac
     4561 ctccgtgtga aatatgaaga ggggccggtg aagccaccgg cccctcgcaa ataacccgag
     4621 agattctcct gaaccggaaa acgtgccgcg ccgtccggtg agagggtggc ccctcttctt
     4681 tgtacttatt gtaagaaagc tgaatgaata tatcacacag ccgggaaaaa ggcaatattt
     4741 tcctccgtcc cttgttttcc tccgtccctg aagaatgtat cagatggacg gctcgcccct
     4801 gcctgccccc tctgtggaga accagtgccg ggccacaagt gccgcgaaga aaacgaagct
     4861 tgaaatcctc agaattctca gcggattgac catgagaatc cccgccagga taaacagcgc
     4921 tctttccagg aaggacatat cccttttgaa cattcctatg atgccgcagg agagggaaaa
     4981 cacgccgata atggcggtga caacgtcaag aagatacttc ggcagaacga agtctatgaa
     5041 aagaagatcg gggttgtaga cgaaaagata gggcaaaagc aagccggaaa gagccagccc
     5101 gagggcggac acggccacct tgaagggacg ggctcccgcc agcccggcag ctgtatagct
     5161 cgtgagtgcc acgggaggaa ccacagcgga catggtgccg tagtagaagg cgaagaaatg
     5221 ggctgccagg ggatggactc ccatctgctg gagtgccgga gcggcaatcg ttgcggtaat
     5281 gatgtagcag ggagttgcgg gaaggcccat gccgaggata attgctgcga tcatgcacag
     5341 gatgagggcg gcccagagtt ttcctcctgc gaacataatg acgttcatgg caatcacctg
     5401 gcccaacccc gtcatcccca cggcaccgac gatgaagccc actatggcgc aggaaatccc
     5461 gaccggtgcg gcactccggg ttccgctgtc cagagcctct atgacctccc gaatacccat
     5521 tctggtagac cgcttcaggg aagaaaccag aacaatggca aataggccga gaaaggcgga
     5581 atagagaggg gtgtaccctg ctacgagaag gtaaatgatg accacaaggg gaatggtcat
     5641 gtgccctttg tcgagcatgg tcgccttcag ggatgggata tctccgggct ccatgccctt
     5701 cagccccctc ttctttgctc tcaggtccac catgaacatg atggcgagat aatacaggag
     5761 cgcgggagtg aagccggcga tcatgatttt cacgtaggga attcccagga aggtgctcat
     5821 gatgaaagct gatgctccca tgatgggcgg catgagaatg cctcctgtac tggcagccgc
     5881 ttcgacggcc ccggcgaaat gaggcatgta ccccaccctc ttcatcaggg gaatggtgaa
     5941 tgcccccgtc gtggcgacat tggcctgggc gcttccggaa atactcccca tgagagccga
     6001 ggctaccact gccaccttgg cgggacctcc ccggtatctc ccggcgagag cgagggagaa
     6061 gtcgttgaag aactcgctgg tcctggaggc cgccaggaat gctccgaaga ggataaacat
     6121 gaacacatag ctggaggaaa ccatgagggt aactccgtaa attccctggt ccgtgagagc
     6181 catgcggatg atgattcttt cccagttgaa tcccctgtgg gcgaaaagcc cggggaagta
     6241 gggaccgaga cgggtatagg caaggaaaag aaggctgaga accgtcagcg ggaggccgac
     6301 tgcccttcgg ctcgcttcca cgaggagggc catggtgacc acggcgaccg caagatcggc
     6361 aaacgttgcc tggaggttcg tctgaagcag cctgtcatag gtgatgagca gccagaccat
     6421 gcacccgagg gaaattcctg ccagaaccca gtcgggcatg gacggccgtt cccttggtga
     6481 tccggcaaag gccggataga aaagaaagat gatcgccatg agggttccga gatgaattgc
     6541 attcatcttg atggcaatga gcagaccgat gctgttcatc cagaaatgga caagggctgt
     6601 agaaacggta aggacgaggg ccgccctgcc tgcagttccg ttcagttttc tcgttgggat
     6661 ggattctccc gggagaattg cggaaagaag ttttttcatc cggtttcgct cctccgaaaa
     6721 aagggcggca tgggacaaaa gaagcccacg ccgcccggat ggctggtaaa atcctgccct
     6781 gcaatcaggg aagaagttcc tggggaatgg tcatcccaat ttccctgaag aacttgacgg
     6841 caccgggatg aagaggagca ccgaaaaggc cgtccacggg attgtcgaaa tcaaccttcg
     6901 taaaggctgc atgctccttc ttcagatctt ctttcttgct gtacacgatc ttcagcatgt
     6961 catagacgag ctcttcgggc acatccttcc cgacgagaag ggcggacttg attccggcca
     7021 ccctgagttc cttctcctgg tttggatagg ttccggcggg aataagcacc gcggcataaa
     7081 agggagcttc ctttttcagt ttcgcaatat catcgggaga gaactcgagc atgttcactt
     7141 cctgtcgccc cgcataaagt tccgccaccg cggcaacggg aacgccggct tcggcgttga
     7201 aggcgtcaag ctggccgttc tgaagggcct gggatgcttc actgtagccc acgtactccg
     7261 ctctgatgtc gtcgaaagta agccccgcga gggccttcag cagaaccctg ctgctgaatt
     7321 ccgttccgga ggcggcagga cctacggcga ccttcttgcc cttcaggtct gcccatgttt
     7381 tgatgccgga ggagccgctc accacgaact gggtcacatc aggccacagc cccatgacgt
     7441 agcgcaggtt ttcgatcttc ttcccttcgt agcggaccgt tccggtatag gcaaaaccgg
     7501 tgaggttcgc catggcaatg gccatctcgg cctcgctgcg ctgcatcatg tccaggtttt
     7561 ccacggttcc tcccgaactc tgggcggacc atttgtaccc cttgtccata agtgccgcat
     7621 tgaagtgatt ggcgaggatg gtgccgatag gatagtatgt tcctccggtg gagcctgtga
     7681 cgatggtgac aaacttgccg gaggctaagg ccgaacctgc ggcggagaga aggaaaagcg
     7741 cgagacagaa caggaccagg atctttttcg ttgacttcat gccttttcac cctttctctg
     7801 attatgagat ctctcgttgc tcaatggcag tgttccggat ccttaatgaa gaacgcataa
     7861 aattgtagca gtagaagagg agggattcaa catattccgc tcaatgaaac aggtcccgac
     7921 catttgttac gccgggacct ttgtataaac gttcttttat atctgcaccc cagataaatt
     7981 ccggatcaga tttgtctctt ccaggatgtc tccccggttc tcaggacttg aggggattga
     8041 actcctccgc caggacctcc ctgaactgct tcatggacat ggacccgaga tcgcccctgc
     8101 tccgctcccg gggagccacg ccgccggatt ccctttcctt ctcccccacc acgaccatgt
     8161 agggaatttt ctgggtctgg gcgtcccgga tttttttgcc gagcttctca tcccgttcgt
     8221 ccacttcaac ccgtattccc gaagcccgca gttccgccgc cacggtaccg gcatagtccg
     8281 caaattctcc ggaaacggga agaatccgca cttgtacagg agcgatccag taggggaaag
     8341 atccagcgaa gttctccaca agaatgccga agaaccgctc aaggcttccg aggacggtcc
     8401 tgtggagcat aacgggacgg tgttccttgc cgtcggctcc aacgtaggta aggtcaaatt
     8461 tctcgggcat ctggaagtcc agctgtatgg tgccgcactg ccatgttctc ccgatgcagt
     8521 cctcgagatg gaagtcgatc tttggtccgt aaaaagcccc gtccccggcg ttcagcctgt
     8581 attccgaccc ggtctcttca agggcctgct tcagggcatt ctcagcaatg gcccactgct
     8641 cctccgatcc catggagttc tccgggcggg tggacagttc catggtatac ctgaagccga
     8701 acacatccct gtagatgtag tcgcagagct tcatgatacc gatgatttcg tccttcacct
     8761 ggtccggacg gcagtagaga tgggcgtcgt cctgggtgaa gcaccggacc ctcatgagcc
     8821 cg
//
LOCUS       DAOTHD010000005         8467 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_10069_length_8467_cov_3.319306, whole genome shotgun sequence.
ACCESSION   DAOTHD010000005 DAOTHD010000000
VERSION     DAOTHD010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 8467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8467
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10069_length_8467_cov_3.319306"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /gene="rplU"
                     /locus_tag="P5219_00180"
     CDS             <1..216
                     /gene="rplU"
                     /locus_tag="P5219_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRX25200.1"
                     /translation="LDKVLFVGGDDGISVGAPYVQGASVKASVVTNGKDRKVLVFKYK
                     SKKNYRRLRGHRQHFTEIRIDSITPGA"
     gene            220..561
                     /locus_tag="P5219_00185"
     CDS             220..561
                     /locus_tag="P5219_00185"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HRX25201.1"
                     /translation="MTEISVGLQAGRKVAIVAAGHTGFSERGSDVVCAGVSALVQALY
                     YGFHEVLKQKELRSSVDKKNAKMSLDWAECPASEVDVLAETIIGSLKEIARVYPDHVR
                     IVEVHVDELDF"
     gene            545..832
                     /gene="rpmA"
                     /locus_tag="P5219_00190"
     CDS             545..832
                     /gene="rpmA"
                     /locus_tag="P5219_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRX25202.1"
                     /translation="MNWIFDLQFFAHKKGQGSSTNGRDSEGKRLGIKKYAGQAVKAGN
                     IIVRQRGTKVHPGKNVGIGKDDTLFATSAGVVNFETKGSRKFVSVVLELAE"
     gene            997..2340
                     /gene="obgE"
                     /locus_tag="P5219_00195"
     CDS             997..2340
                     /gene="obgE"
                     /locus_tag="P5219_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRX25203.1"
                     /translation="MKFVDFVKIEVSGGRGGNGCLSFRREKFIPKGGPDGADGGKGGD
                     VILEAAPGTLTLADFEYEKKFKAGHGQPGKGGLKSGAAGEDRIITVPCGTIVYDDDTG
                     DILADLVEPGERVVIARGGRPGRGNAHFANSVRKTPRFAEKGDEGEEKRLLLELKLIA
                     DVGLVGFPNAGKSSLLAAISTAKPKIAGYPFTTLSPNLGVLSVDDQKIVIADVPGLIE
                     GAHENKGLGVYFLRHIERTRFLVHVLDLASGSAEDVLDQWRIVREEFRAYNAMAGDRL
                     PGEEREDLLSRPCIVVGNKIDLAGTAEVDRDVRTFMDREGIPYFTVSAMTGEGIGEII
                     PAIVSLARENPRPAGETRLVVPETPVPVRQRRKKEPVSIVRLQDGAGFRVVHEGLERI
                     LQRYDFEHEDAILKFARLVRRFRIEELLEENGAQKGDKVYIGDLEFDFEPDKVME"
     gene            2358..3002
                     /gene="nadD"
                     /locus_tag="P5219_00200"
     CDS             2358..3002
                     /gene="nadD"
                     /locus_tag="P5219_00200"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HRX25204.1"
                     /translation="MADVQGRARKILRKIGIMGGTFDPIHFGHLLAAEESRTALDLDE
                     VIFIPAGDPAHKRERKTASPEDRYVMTLLTTLGNQKFTPSRIEIDRKEPSHTVDTLRE
                     MRHWYAPEKVDFFFITGLDAVLGITTWKEHEALPGLCRIVAVNRPGYVPQKLESLPDE
                     MRSAIIPLEIPLLSISSTEIRQRIEQGRSIKYLLPEAVEQYIYKKGLYRRKMEV"
     gene            3007..4287
                     /locus_tag="P5219_00205"
     CDS             3007..4287
                     /locus_tag="P5219_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRX25205.1"
                     /translation="MKIMKIAAILLLAVSSAFAGAAMRVRTFVDPKPQTIKESISFTE
                     ASGTANILLIGIDDVDGGRRADAIAFATVDIDNKIVRVMSVPRDTRVQIPGKGWDKIN
                     HAYAYGGVEKLRETVVNFLGLPVNYYVLVNYDTFPSIIDLIGGVEVDVERRMVYNDYA
                     GKLFINIPKGFQKLDGKNALHYVRFRHDAMGDIGRVKRQQEFIKAVLKKVQTPSMIPK
                     IPELAQKAIEMVNSDMTPAQALQLASYLADLSGENLHFFTLPGKAAYISNLSYWIGDT
                     TEASALLTGLPEKKADGPVSGDRSPEGQDSPAQNSGIDLESLIASVTTPVSVLNGAGK
                     SGLGKDTATALQKIGIDVAHIGNAKHFDYRTTSIQVPEKGAEAVRNTAQALGEIAGVG
                     KNLISKSSAVPHVTIIIGKDSEKVLERLGGLARR"
     gene            4332..4688
                     /gene="rsfS"
                     /locus_tag="P5219_00210"
     CDS             4332..4688
                     /gene="rsfS"
                     /locus_tag="P5219_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRX25206.1"
                     /translation="MKKENRLREFEYLYNALADKRGLDIVAMDMEGSAAISDTFVLVT
                     ANSEIHMGTLRDAALEAFHTQGMQTTVEGYDSSQWRLIDAGEVVVHVFSRAGRDHYKL
                     EKLWGDAPTFNFTYED"
     gene            complement(4803..5579)
                     /gene="tpiA"
                     /locus_tag="P5219_00215"
     CDS             complement(4803..5579)
                     /gene="tpiA"
                     /locus_tag="P5219_00215"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRX25207.1"
                     /translation="MKKIYLYGNWKMNMLPEEGEEFCRSLADRISGRLYGSDLVDICL
                     FPPFLAIPAVLKALPAGPVSAGAQDGYFEDRGAFTGAVSMAMVRSVGCSHVLVGHSER
                     RHIFGETNEVVARKLEKALNSGLKTVLCFGETLEEREGGKTIQVVQDQLLSAFQSVPE
                     VRARDLVLAYEPVWAIGTGKNASPDDAQEVCAFAAKLALEAFGNGSLIPVLYGGSVKD
                     SNAAELLSRQDIYGALVGGASLKVDSFLAIYENYRKAFTG"
     gene            complement(5609..6799)
                     /locus_tag="P5219_00220"
     CDS             complement(5609..6799)
                     /locus_tag="P5219_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRX25208.1"
                     /translation="MKLRLFSHQDTAEKKVLLRVDFNVPVKNGEVTDFTRISAHRDTI
                     SALLKSNAKVALCSHLGRPKGKIVPEMSLSHVAAAVEKVLGHPVKFCRECIGPEVETA
                     LASLSPGNLLLLENLRFHPQEQENDAQFAASLAAPFDVFVMDAFSASHRADASTNAIM
                     KILPSFSGFLLEREVNMLSAVSEAPEKPFVLILGGAKVSDKIGVVEHLMDKATAILIG
                     GGMAFTFLKAGGSGIGRSLFESEKAGFALEMMKKAREQGVEILLPVDVVAGSSPDTSQ
                     GERIVPADEIPEDLMGLDIGPKTAELFANKAAGARTVLWNGPMGVFENPLFEKGTRTV
                     AEGVARSTHNGGLTVIGGGDTASAAKKLGFDRNVSHVSTGGGASLEFCEGRQLPGIMP
                     LLAE"
     gene            complement(6824..7744)
                     /gene="whiA"
                     /locus_tag="P5219_00225"
     CDS             complement(6824..7744)
                     /gene="whiA"
                     /locus_tag="P5219_00225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HRX25209.1"
                     /translation="MTNTDSGKILSSFMWDEWLTAACKDRTEALGEIRGILQGMRITE
                     RTGKELLLSSSRLWLFRRLIKLWGEADLGSIHDFPSLLSIPRSLKGKVFLRFPEKLLF
                     LHENRMDDMSRFERKRPDWLRGLWGSCGALYIPKSGYYLSFRVPSRETELTAAAMLKK
                     SGFSFGRRHIQGKHEITLRNQEEIVTFLIRIGLVKTSLRMEEKAIVRSMRNRANMLVN
                     CDSSNIRKSLDAATRQLDLARAAVSAPGFESLPDVLKNLVMSRITNPSVTLEELGKLQ
                     SPPISKSTVQYRWKKLEHVMGSAAESGERN"
     gene            complement(7750..>8467)
                     /gene="yvcK"
                     /locus_tag="P5219_00230"
     CDS             complement(7750..>8467)
                     /gene="yvcK"
                     /locus_tag="P5219_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HRX25210.1"
                     /translation="AGHSLGNLMLLAISEQTGDFRRAVEEMNNLLAIRGRVLPVTTEA
                     VSLVAETVAGERIRGELSISEHGSKIRKIWLEPLDARPVGDVLRAIDEAEILVLGPGS
                     LFTSVIPNLLIPRVAERLRKAAVPKVYICNLMTQPGETEGFSLAEHVRWIAAAMGVPP
                     DYVIANAGEIPLHLMEKYGNEGAVPLVATDRDRDELDDMGCILLEGNFVHIIEGSLLR
                     HDGQGLSHLLIRLCRELKED"
BASE COUNT         2005 a         2211 c         2373 g         1878 t
ORIGIN      
        1 ctcgacaagg ttcttttcgt gggtggagat gacggtatat ccgtcggggc tccctatgtc
       61 cagggagctt ccgtaaaggc ttcagtggtc accaacggaa aagaccgcaa agttcttgtc
      121 ttcaagtaca agagcaagaa aaattaccgt cgcctgagag ggcacaggca gcattttacg
      181 gagattcgta tcgactccat caccccggga gcctgagaag tgacggaaat ctccgtcggc
      241 ctgcaggccg gccggaaagt tgccattgtg gcagccgggc atacggggtt ttccgaaaga
      301 ggttccgatg tggtctgcgc aggagtttcc gcccttgtcc aggcactgta ttacgggttt
      361 catgaggtgt tgaagcagaa ggagctgagg tcctccgttg acaagaaaaa tgcgaagatg
      421 tccctcgact gggctgaatg tccggcctca gaggtggacg ttctggcgga gaccattata
      481 ggctcgctga aagaaattgc ccgggtttat cccgatcatg tccgaattgt ggaggtgcac
      541 gtagatgaat tggatttttg atctgcagtt ctttgcccat aaaaaaggcc agggaagcag
      601 cacgaacggc agggacagcg aaggcaagcg ccttggcatt aaaaaatacg ccggccaggc
      661 tgtaaaggcc ggaaacatta ttgtccggca gcggggcaca aaggttcacc ccggcaaaaa
      721 cgtcggcatc ggcaaagatg acaccctttt cgccacaagt gctggtgtcg tcaacttcga
      781 aacgaagggc agcaggaagt tcgtttccgt ggttctcgaa ctggccgaat aggcccgtct
      841 tttggaaacc gaaagtagaa cagcatcaaa agggcctttg cttcttgccg tgctgccggg
      901 aaactttgcc tgttttccgg agtgcgggaa gatgtcctgg gtccttttct atttgggaaa
      961 attgaaggga ctcgtggggt tttacccggt gatgacatga aatttgttga tttcgtaaaa
     1021 attgaggtgt ccggaggacg gggcggaaac ggatgcctga gtttccggag agagaaattc
     1081 ataccgaaag gcggccctga cggcgcggac ggaggaaaag gaggagatgt catcctcgag
     1141 gctgcccccg ggacgctgac tttggctgac ttcgaatatg agaaaaaatt caaagccggt
     1201 cacggccagc ccggcaaggg tgggctgaaa tcgggcgccg cgggagaaga ccggatcatc
     1261 accgtcccct gcggtacaat tgtttatgat gacgatacgg gagatatact tgcagacctg
     1321 gtcgaacccg gcgaaagggt tgtaatcgca cgggggggaa gacctggaag aggaaatgcc
     1381 cattttgcaa actctgtgag aaaaaccccc cggttcgccg aaaagggaga cgaaggagaa
     1441 gaaaaacggc ttcttcttga actgaagctt attgccgacg tggggctcgt cggttttccg
     1501 aatgccggaa aatcgagcct ccttgcggca atttccacgg caaaaccgaa aattgccgga
     1561 tatccgttca caaccctttc gcccaacctc ggcgtgctgt ccgtggatga ccagaaaata
     1621 gtgatcgccg acgttccggg gctaatagaa ggggcccacg agaacaaggg gttgggagtg
     1681 tattttctcc ggcatatcga gagaacccgg tttctcgttc acgttcttga tctcgcctcc
     1741 ggctccgcgg aagatgttct tgaccaatgg agaatcgtca gggaagaatt ccgcgcatac
     1801 aacgccatgg cgggagaccg tctccccgga gaagaaaggg aggatcttct gtctcgtccc
     1861 tgcatcgtcg tgggaaacaa gatcgacctg gccgggacgg ctgaggtgga ccgggatgta
     1921 aggaccttca tggaccggga aggaatcccg tatttcactg tcagcgccat gacaggtgaa
     1981 ggtatcggcg aaatcatccc ggccatcgtt tcgcttgcac gggaaaatcc ccgtcctgca
     2041 ggggaaacca ggctggtggt tcctgaaacg ccggttccgg tgcggcagag aagaaaaaaa
     2101 gagcccgtgt ccattgtcag acttcaggac ggagcaggct tcagggtggt ccacgaaggg
     2161 ctcgagagaa ttctgcagcg gtatgatttt gagcatgagg atgctatcct gaaatttgcc
     2221 cgcctcgtcc ggagattcag gatcgaggaa ctcctggaag aaaacggcgc ccaaaaaggg
     2281 gataaagtgt atataggaga ccttgaattt gattttgaac ctgacaaggt catggagtga
     2341 cggctgacag cgggggaatg gctgacgtgc agggaagagc ccgaaagata ctgagaaaaa
     2401 taggaatcat gggcggaacc ttcgacccga tccatttcgg ccatcttctc gcagcagaag
     2461 aaagcaggac agccctggac ctggacgaag tcatcttcat tcctgcgggt gatcctgccc
     2521 ataaaagaga gcgaaagacg gcatctcccg aagacaggta tgttatgaca ctcctgacga
     2581 ccctggggaa ccagaaattt accccttcaa ggatcgagat cgaccgcaag gaaccgagcc
     2641 ataccgtgga taccctgagg gaaatgcggc actggtatgc ccctgaaaaa gttgattttt
     2701 tctttatcac gggccttgat gcggtactcg gcattacaac gtggaaagag cacgaagccc
     2761 ttcccggcct ctgcagaatt gtcgccgtca acaggccggg gtatgttccc cagaagcttg
     2821 agtctctgcc ggatgaaatg aggagcgcca ttattcccct ggaaattcct cttctttcca
     2881 tctcaagcac ggaaatccgc cagaggatag agcaggggag aagcatcaag tatcttcttc
     2941 ccgaggctgt ggagcaatac atttataaaa aagggctcta caggagaaaa atggaggttt
     3001 gagccgatga aaataatgaa aatcgccgcc attcttctgc ttgccgtttc gtcagcattc
     3061 gcgggcgctg ccatgcgggt ccgcaccttt gttgatccga agccgcagac cataaaggaa
     3121 tcaatctcct tcacggaagc ttcgggaacg gcaaacatac tcctcatcgg aatcgatgac
     3181 gtggatggag gcagaagagc cgatgcaatt gcctttgcca ccgtcgatat cgacaacaag
     3241 atcgtcaggg tgatgtcggt cccgagggat accagggtac agattcccgg caaaggatgg
     3301 gacaaaatca accacgccta tgcctacgga ggagtggaga aactccggga aacagtggtc
     3361 aatttcctgg gccttcccgt aaactactac gtccttgtca actatgacac cttcccgtcc
     3421 atcatcgacc tcataggcgg ggtagaagtg gatgtggaac ggcgtatggt ctacaacgac
     3481 tatgcgggaa aactctttat caacatcccg aaggggttcc agaagctcga cgggaaaaat
     3541 gcccttcact acgtccgttt ccgtcatgat gccatggggg atatcggaag ggtgaagcgc
     3601 cagcaggaat tcataaaggc tgtcctgaaa aaagttcaga cgccgtccat gattcccaag
     3661 attccagagc tggcccagaa agccatcgaa atggtgaact ccgacatgac tcccgcccag
     3721 gcgctgcagc ttgcttccta ccttgccgac ctttcgggag agaacctcca tttctttact
     3781 cttccgggaa aagctgccta tatttccaac ctcagctact ggattgggga tactaccgag
     3841 gcgtccgccc tgctgaccgg acttccggaa aagaaagcag acggaccagt ttcaggcgac
     3901 cggtcgcctg aaggacagga tagccccgcc cagaacagcg gcatcgatct tgaatcactg
     3961 atagcttcgg tgacgacacc ggtctcggtg ctcaacgggg cgggaaagtc cggactcggc
     4021 aaagacaccg cgacggctct acagaaaatt ggcattgatg tggcgcacat tggaaacgcg
     4081 aagcattttg actatcggac cacgagcatc caggtgcctg aaaaaggcgc cgaagcggtg
     4141 cggaatacgg cccaggcact aggggaaata gcaggggtgg ggaagaacct catatcgaag
     4201 agctcggctg tccctcatgt tacaatcatc atcgggaagg atagtgaaaa agttctggag
     4261 agacttggcg gattggcccg ccgatgacct gaaagaatca gacctcaacc actaaaggag
     4321 aaggtgaagg attgaagaag gaaaaccggc tcagggaatt tgaatacctg tataacgccc
     4381 ttgctgacaa aagggggctt gatattgttg ccatggatat ggaaggatcg gctgccatct
     4441 cggatacctt tgtcctggta acggccaatt cggaaatcca catgggaact ctcagggatg
     4501 cggcccttga agcatttcac acacaaggta tgcagaccac cgtcgaagga tatgacagct
     4561 cccagtggcg tctcatagat gcgggcgaag tggtggttca tgtcttcagc agggccgggc
     4621 gggaccatta caagctcgaa aagctctggg gcgacgcgcc gacgttcaat tttacatacg
     4681 aagactgaag tgacgacaac aaatgcagct gccgtacaag accggaagct gcattttttt
     4741 gaagatccta tatgtcctat aatatatctt atgttgcgtt ttgctgttca ttgaactgtt
     4801 ttttacccag tgaaggcctt cctgtagttt tcataaattg ccaggaaaga gtccactttc
     4861 agcgaagctc ctcctaccag cgctccgtag atgtcctgcc tggaaagaag ctcagccgca
     4921 tttgaatcct tgacgcttcc tccgtacagc acgggaatca gtgacccgtt gccgaaagcc
     4981 tcaagggcca gtttcgccgc aaatgcacag acttcctggg catcatcggg gcttgcgttt
     5041 tttccggtcc cgatggccca tacaggttca tatgcaagga cgagatcccg ggcccggact
     5101 tccggcacgg actggaaagc tgaaagaagc tgatcctgca ccacctggat cgttttgccc
     5161 ccttcccgtt cttccagggt ttccccgaaa caaaggacgg tcttcagccc ggagttcagg
     5221 gctttttcca gtttcctggc cacgacttca ttggtttctc caaatatgtg acggcgttca
     5281 ctatgcccca ccaggacgtg agagcatcca acggaccgga ccatagccat tgacacggca
     5341 ccggtgaatg cgcccctgtc ctcgaaatag ccgtcctggg cacctgccga aacgggaccg
     5401 gccgggagag ccttcagaac tgccgggata gcaaggaagg gcgggaaaag gcagatgtcc
     5461 acaagatcac ttccatacag tcttccggaa atcctgtccg ccagggagcg gcagaactct
     5521 tccccttctt ccgggagcat gttcattttc cagtttccgt aaagataaat ttttttcatg
     5581 tccggcctcc tcacgggatg ttttctttct actcggcaag caggggcatt attcccggga
     5641 gctgcctgcc ttcgcagaat tcaaggctcg ctcccccgcc ggtggaaacg tgggaaacgt
     5701 tgcggtcgaa cccgagcttt ttcgctgcgg aagccgtgtc gccgcccccg ataaccgtca
     5761 gtcccccgtt gtgcgtgctc cgggcgactc cttcggcaac agttcgcgtt cccttctcga
     5821 acagcgggtt ttcaaatact cccatgggac cgttccagag gactgttctg gctcctgccg
     5881 ccttgtttgc aaagagttcc gctgtcttcg ggccaatgtc gagacccata aggtcctcgg
     5941 gaatttcgtc ggcaggcacg atcctctcgc cctgtgatgt gtccggggag gatcctgcga
     6001 ccacgtcgac gggaagaaga atttcgacac cttgttcccg ggcttttttc atcatttcaa
     6061 gcgcaaatcc ggctttctcg gattcgaaaa gggaccgtcc gataccggat ccccccgcct
     6121 tcagaaaagt gaaggccata cctcccccta tgagaatggc ggtcgccttg tccataagat
     6181 gctccaccac tccgatttta tcggacactt ttgcccctcc gaggatgaga acgaagggtt
     6241 tctccggggc ctcgctcact gctgagagca tgttgacctc cctctcaagc aggaaccctg
     6301 agaatgaggg cagaatcttc atgatggcgt ttgtggacgc gtctgccctg tgggaggcac
     6361 tgaacgcgtc catgacaaat acgtcgaagg gggctgcgag agatgcagca aattgggcgt
     6421 cgttttcctg ttcctgggga tgaaaacgaa gattttcaag cagcagaaga tttccagggg
     6481 agagggatgc aagggctgtt tcgacctccg gtccgatgca ctccctgcag aatttgacag
     6541 gatggccgag aaccttttcc actgctgctg cgacgtggga aagggacatt tcgggcacaa
     6601 ttttaccctt tggcctgccg aggtgggaac agagggcgac cttcgcgttt gacttcagca
     6661 gtgcggaaat agtgtccctg tgagccgaga tcctggtgaa atccgttact tcaccgtttt
     6721 ttacaggtac gttgaaatcc accctgagca gcaccttttt ttctgcagtg tcctggtggg
     6781 aaaacagacg gagtttcatg gacaaccttc ctccttccga taatcaattc ctctcgccgc
     6841 tctctgcggc tgaacccata acatgttcga gctttttcca ccgatattgt acagtacttt
     6901 tactgatggg aggcgactgc agcttcccga gctcctcgag ggtgacgctg ggattggtga
     6961 tcctcgacat cacgaggttt ttcaggacat cgggcaggga ttcgaaaccc ggcgcggaaa
     7021 cagccgcccg ggcaaggtca agctgccgcg tagcggcatc aagggatttg cggatgtttg
     7081 acgagtcgca attcaccagc atgttcgccc tgtttctcat ggatcggacg atggcttttt
     7141 cctccatgcg gagcgatgtt ttcacaaggc ctattctgat gagaaacgtg acgatttcct
     7201 cctggttgcg gagggtgatt tcatgttttc cctgtatatg cctcctgccg aaggaaaagc
     7261 cgcttttttt cagcatggcg gcggcggtga gttccgtttc cctggaagga accctgaaac
     7321 taaggtaata gccgcttttg ggaatgtaga gtgccccgca gcttccccag agacctcgca
     7381 gccagtcggg ccgtttcctt tcaaaacggg acatgtcatc catccggttt tcgtgcagaa
     7441 agagcagttt ttcgggaaag cggagaaaga cctttccctt gagcgatctc ggaatgctca
     7501 gaagggacgg aaagtcgtga atggagccga ggtccgcttc tccccagagt ttgatcaaac
     7561 gtcggaaaag ccacagcctg ctgctggaaa ggagcagctc ttttccggtt ctttcggtga
     7621 ttctcattcc ctggagtatg ccgcggatct cgcccagggc ttctgtccgg tccttgcagg
     7681 ctgccgtgag ccattcatcc cacataaagg aagagagtat tttccctgaa tcggtgtttg
     7741 tcaaggagat cagtcctctt tcagttcgcg gcagaggcgg ataaggaggt gggacaatcc
     7801 ctgcccgtca tgccggagaa gagagccttc aatgatgtgt acgaaattcc cctcgaggag
     7861 gatgcatccc atgtcatcca attcatccct gtccctgtcc gttgccacca gtggaacggc
     7921 cccttcgttt ccgtactttt ccatgaggtg gagtgggatt tcaccggcat tggcgatgac
     7981 ataatcagga ggaacgccca tggcggctgc aatccaccgt acgtgttcgg ccagggaaaa
     8041 gccttccgtc tcccccggtt gggtcataag attgcagatg tacactttcg ggaccgccgc
     8101 ttttctcagc cgttccgcta ccctgggaat gagaagattg gggatgacgc tggtaaaaag
     8161 gctccccggt cccaggacaa gaatttcggc ttcgtcaata gcccgaagga catcgcccac
     8221 agggcgggca tccaggggtt cgagccagat tttcctgatc ttgcttccgt gctcggaaat
     8281 ggaaagttct cccctgattc tctctccagc aacggtttcg gcaaccaggg acacggcttc
     8341 cgtagtcacc gggagaaccc gtccccgtat agcgagaagg ttgttcatct cttcgaccgc
     8401 ccgtcggaaa tcaccggtct gttcagatat ggccagaagc atgagattgc cgaggctgtg
     8461 cccggca
//
LOCUS       DAOTHD010000006         7369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_12042_length_7369_cov_3.009024, whole genome shotgun sequence.
ACCESSION   DAOTHD010000006 DAOTHD010000000
VERSION     DAOTHD010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 7369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7369
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12042_length_7369_cov_3.009024"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="P5219_00235"
     CDS             complement(<1..1295)
                     /locus_tag="P5219_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRX25211.1"
                     /translation="MLQWIQIKGAREHNLKNIDVDIPKNKLVVITGPSGSGKSSLAFD
                     TLYAEGQRRYVESLSVYARQFLGVQNKPDVDDISGLSPAISIEQKGVSHNPRSIVGTV
                     TEIYDYLRLIFARVGKPHCPSCGKEVVRYSLDEIVDILFRSFPEERVEILAPLVRGKK
                     GEYRNLLSQTRDKGFLRVRVDGAVLWLEEEISLDKNKRHTIEVIVDRLRIQEDRRSRI
                     AEAVENALSLSGGYVVAVPEKGEEKLLTENFTCPSCDISLPEIEPRLFSFNNPYGACP
                     DCSGIGSHQFFSEELAIDPERSLSEGAVLPWKKKHYMLTKLEKFAAKKEWDLSGPYGK
                     LPGTVKEFILRGSDERIPLMFREGGEERSYMGRYEGLLPWLDARWKETESEAVLEELA
                     TYRVDDECKTCGGLRLKPEALNVKIQEYGIGDFVSMPID"
     gene            complement(1234..3273)
                     /gene="uvrB"
                     /locus_tag="P5219_00240"
     CDS             complement(1234..3273)
                     /gene="uvrB"
                     /locus_tag="P5219_00240"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRX25212.1"
                     /translation="MNTEEKKFNLISEWAPSGDQPEAIRRLSAGVSERHRYQTLLGVT
                     GSGKTFTVANVIAEAGRPVLVLAHNKTLAAQLYSEFKGFFPQNAVHYFVSYYDYYQPE
                     AYVPATDTYIEKDASVNDRIERLRLAATKALIERRDVIVVASVSCIYGLGRKETYEKV
                     IFPFSVGDLWERRAFMEKLLENYYERNDLVVEQGSFRARGDIIEIFPAYGETALRICF
                     FDDEIEKIEEFDPVSGKVKENLLHASIFPAQHYVTDRDAIDRSIEPIRAEMEEQAAAF
                     EKQGKFLEAQRIRMRTQYDLEMLLETGYCSGIENYSRYLDGRNPGEPPGTLLDFFPGD
                     FLLVVDESHITLPQVRGMFNGDRARKLTLVENGFRLPSCLDNRPLEWAEFEKYMKQAI
                     FVTATPGDYEFRVSGKVVEQLIRPTGVLDPEVEVLPASGQVDDLIGRLREITSRGERA
                     LITTLTKKSSEDLAEYLADLQFKVKYIHSELNTFERAELIRDLRNGDISVLVGINLLR
                     EGMDLPEVSLVAILDADREGFLRSERSLIQMMGRAARNTAGKVILYADVITDSIRNAT
                     AETARRRALQMKYNEDHGITPASIRKDVISLLPEELMADAGSGYLRKREKDTLDGIPP
                     AELEKMMWQAVERLDFEKAARLRDALASLEGKEWNRVAMDSNKRSKGTQPKKHRR"
     gene            complement(3365..5281)
                     /gene="ftsH"
                     /locus_tag="P5219_00245"
     CDS             complement(3365..5281)
                     /gene="ftsH"
                     /locus_tag="P5219_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRX25213.1"
                     /translation="MGRLVKNLGLYLILIVLVVSLVNIFLSPTQGPQQIQEIGYSTFL
                     SEVSSGRINTVTVRENSIKGKFTDGREFVSYVVGVGDLAKEVAQKGVNVEVEPPQKTP
                     WWANMLSSLFPTLLLIGVWIFFLYNMQGGGGKVMNFAKSKAKLFLDNRPKVTFNDVAG
                     CEESKEELSEVVYYLKDPSRFTALGAKVPRGILLLGPPGTGKTLLARAAAGEADVPFF
                     SVSGSDFVEMFVGVGAARVRDLFEQARKYQPCIIFIDEMDAVGRQRGAGLGGGHDERE
                     QTLNQLLVELDGFDESTGIILIAATNRPDILDPALLRPGRFDRHIVVDRPDVKGREAI
                     LQVHVREKKLALDVDLEVLARRTPGFVGADLANLVNEAALLSARNGEKEISMRNFEEG
                     IDRVIAGPERKSRLVSEKEKKIIAFHETGHALVAKLIPSCDPVHKISIIPRGHMALGY
                     TLQLPEEDRFLMSRSDLLDKICVLLGGRVAEELHFGDVTTGASNDLERATQIARQMVT
                     EFGMSDKLGLVKLGHKHQEVFLGRDIGEDRNYSDEVAYAIDQEVKKIIDSCYGRVHHL
                     LAENADEMAKVAQVLLEKEVIEGKELTLLLGMEEKEEKKDTPAEKEKPERESSGVLSE
                     DSRTPNVLGEAPAD"
     gene            complement(5311..5865)
                     /gene="hpt"
                     /locus_tag="P5219_00250"
     CDS             complement(5311..5865)
                     /gene="hpt"
                     /locus_tag="P5219_00250"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRX25214.1"
                     /translation="MEYKVSDVLIPKEEIEKRVAELGARIAADYEGQELVVVGILKGA
                     VVFMADLVRNIPPSVNISLDFMAVSSYGDSTKTSGIVRIVKDLDSSIKDKNVLIVEDI
                     VDTGLTLSYLVKLMHERQPGSVRVCALLDKEERRKIHVDVAYKGFSIPDRFVVGYGLD
                     YAGKWRNLPSVHIVDVSGESRAAL"
     gene            complement(5855..7255)
                     /gene="tilS"
                     /locus_tag="P5219_00255"
     CDS             complement(5855..7255)
                     /gene="tilS"
                     /locus_tag="P5219_00255"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRX25215.1"
                     /translation="MDTMEKSPRPDIPFDYSLARLKKEFTQTGIEQGWLHTESPILLA
                     VSGGSDSMAMLWLFSRFRNTNLVVAHLDHGIRGEEGKEDSRFVASMAARFGVRYISES
                     LPVPSLLQKGESLEDGARRIRYRFLEETGKALGAWGIGVAHTKDDSAETFLHNLFRGT
                     GVRGLTGIPEKRGLIFRPLLQYSRDYLRQLLRWYGLPWREDSTNEDTAYLRNKIRTVL
                     IPFIEREVNSSARKHILGTAGDLAFFRGREEKLQKSLLRLASVSLPFCSYACPVSFLR
                     KLDGETAAVFLRGAGRILGLKALSRERTETLVGLLRTDGPWCFQWQSSMYVFCSFPYV
                     TWVDPVILNQPDPSGLNVHLEGNQGNFEWNGWQFTWKRETSEKVCAGWMRAVLPWSGI
                     IHVSPLSELQQYKDAWAPRWARRIIPVLRSGSFDWIPFWGRRTSGILNETGDQVAVIQ
                     AEVYHLTAEKGKDYGV"
BASE COUNT         1589 a         2063 c         1907 g         1810 t
ORIGIN      
        1 tcatcgatgg gcatggagac aaagtccccg atcccgtatt cctggatttt cacattgaga
       61 gcttccggtt tgagtctcag tccgccgcat gtcttgcatt cgtcatccac cctgtaggta
      121 gcgagctctt cgaggacggc ctcagattcc gtttccttcc accgggcatc aagccatggc
      181 aggagtccct catatcgtcc catgtaggat ctttcttccc cgccttcccg gaacatcagc
      241 ggaattcgtt catccgatcc cctgagaata aactctttaa cagtcccggg gagcttcccg
      301 tagggcccgg aaagatccca ttcctttttt gcggcgaact tttcaagctt tgtcagcatg
      361 tagtgttttt tcttccacgg gagaaccgcc ccctcggaca gggatctctc agggtcgatt
      421 gccagctcct ccgaaaaaaa ctggtggctg cctataccgg aacagtccgg gcaggctccg
      481 tacgggttgt tgaaagaaaa gagcctgggt tctatctcgg gcagagaaat atcgcatgag
      541 gggcaggtaa aattttccgt gagcagcttt tcttcccctt tttcagggac cgccacaacg
      601 tagcctccgc tgagggaaag ggcgttttcc acggcctcgg cgattctgct ccgccggtct
      661 tcctggatac gaagcctgtc tactatgacc tcgatggtat ggcgtttgtt cttgtcgagg
      721 gatatctcct cttccagcca cagaacggcg ccgtcgaccc tgactcgaag gaatcccttg
      781 tcccgggtct gggaaagcag gtttctgtat tctccttttt ttcccctcac caggggagcg
      841 agaatctcaa ccctctcctc aggaaaactg cgaaagagga tgtccactat ttcatcgagg
      901 gaatagcgca ccacttcctt tccgcaggag gggcaatgag gctttcccac ccgagcgaaa
      961 atcagccgga gatagtcgta aatttccgtg acggtcccga cgatggagcg gggattgtgg
     1021 gatacgccct tctgttcgat ggaaatggcc ggagagagtc ccgaaatgtc gtccacgtcc
     1081 ggtttgttct ggacgccaag aaactgtctc gcatagacgg aaagagattc aacatacctt
     1141 cgctgcccct ccgcgtagag agtatcgaag gcgagggacg atttgcctga tcccgaaggg
     1201 ccggtgatga ccacaagctt gtttttcggg atgtcaacgt cgatgttttt taggttgtgt
     1261 tcccttgctc cttttatttg aatccattgc aacacggttc cattccttcc cttccagaga
     1321 agcaagcgcg tccctgagtc gggccgcctt ctcaaaatca agccgttcaa cggcctgcca
     1381 catcatcttt tcaagttctg ccgggggaat cccatccagg gtatcctttt cccttttccg
     1441 gagataaccg gaacccgcgt ctgccatgag ttcttcgggc aggagcgaaa tgacgtcctt
     1501 ccttatcgat gccggcgtta ttccgtggtc ttcattgtac ttcatctgca gagcccgtct
     1561 cctggcggtc tcggcagtgg cgttgcggat gctgtccgtt attacgtcag cgtagagtat
     1621 aaccttcccc gccgtattcc ttgccgctct gcccatcatc tggataagag accgttctga
     1681 ccggaggaat ccctccctgt cggcatcaag aatagccaca agagaaacct ccgggagatc
     1741 cattccctcc cggagaaggt tgattcccac aaggaccgaa atgtcgccgt tccggaggtc
     1801 ccggatgagt tcggcccttt cgaaggtatt gagctcagag tgaatgtatt tcaccttgaa
     1861 ctgaagatca gccagatact ccgcaagatc ttcagacgat tttttcgtca gggtggtgat
     1921 cagagccctc tcacccctgg aagtgatctc cctgagccgt cctatgagat cgtccacctg
     1981 tccgctcgcg ggaaggacct ccacttccgg atcaaggaca ccggtaggac ggatgagctg
     2041 ctccaccact ttccccgaaa cacggaactc gtaatcaccc ggagttgcgg taacgaaaat
     2101 tgcctgcttc atgtattttt cgaattcggc ccattccagg ggtctgttgt caagacagga
     2161 aggaaggcga aagccgtttt ccaccagggt cagcttccgg gcacggtctc cgttgaacat
     2221 gccccgaacc tgggggaggg taatgtggga ttcatccacc acgaggagaa agtccccggg
     2281 aaagaaatcg agcagtgttc cgggaggttc tcccggattt cttccgtcga ggtatctcga
     2341 gtagttttca attcccgagc agtagcctgt ctcgagaagc atttcaaggt cgtactgggt
     2401 cctcatcctg attctctggg cttcaaggaa cttcccctgc ttttcgaatg ctgcggcctg
     2461 ctcctccatt tcagcccgaa taggttcgat ggaccggtca atggcatccc tgtcggtaac
     2521 gtaatgctgg gccgggaaga ttgaagcatg gaggaggttc tcctttacct ttccggaaac
     2581 cgggtcgaac tcttcaattt tttctatttc gtcgtcgaaa aaacagatcc ggagagccgt
     2641 ttctccgtag gcggggaata tttcgatgat gtctcccctc gcgcggaaac tgccctgttc
     2701 aaccaccaaa tcatttcttt cgtagtagtt ttccagcagt ttttccatga acgccctccg
     2761 ttcccacagg tcacccacgg agaaggggaa aatgactttt tcatatgttt ctttacgtcc
     2821 gagtccgtaa atgcaggaaa cgctcgcaac gacaatgacg tcccgccgtt ctatcagcgc
     2881 cttggttgcc gcgaggcgga gccgttcgat cctgtcattg actgaagcgt ctttttcgat
     2941 ataggtatcc gttgcgggaa cataggcctc tggctggtaa taatcgtagt agctgacaaa
     3001 ataatgcacg gcgttctggg ggaaaaaccc tttgaattca ctgtaaagct gtgcggcgag
     3061 ggttttgttg tgggcgagga cgagaacggg cctgcctgct tcggcgatga cattggccac
     3121 cgtgaaggtt tttccgcttc ctgttactcc gaggagggtc tggtagcggt gcctttcgga
     3181 tacaccggcg gacaaacggc ggattgcttc gggctgatca cctgaaggcg cccattcaga
     3241 aatcaggttg aatttttttt cttccgtgtt cataagaatg agacacctcc ttcccgctgc
     3301 ccggaaaaga aaaagggaac cgggaaagtt cccggctcct ctctttccac tctttctgcc
     3361 gaaactaatc tgctggagct tctcccagga cgttcggagt tcggctgtct tcggaaagca
     3421 ctccggaaga ctccctttcc ggtttttctt tttcggcggg agtgtctttt ttctcctctt
     3481 tctcttccat gccgagcaaa agagtaagct cctttccctc gatgacttcc ttttccagga
     3541 gaacctgggc gaccttcgcc atctcgtcgg cgttttcagc cagcaggtga tggactctgc
     3601 cgtagcagga atcgataatc ttcttcactt cctggtcaat ggcgtatgcc acctcatcgc
     3661 tgtagttccg gtcctctccg atgtcccttc cgaggaatac ttcctggtgc ttgtggccga
     3721 gcttgacgag ccccagcttg tcgctcatgc cgaattcggt gaccatctgc ctggcaatct
     3781 gggtggcccg ttccaggtcg ttgctcgcgc ctgtggtgac atcaccgaaa tgaagctctt
     3841 cggccactct tccgccgaga agaacgcaga tcttgtcaag aagatctgaa cgggacatga
     3901 gaaagcgatc ctcctccggc agctgaaggg tgtagccgag agccatgtgt cctctcggga
     3961 tgatggaaat cttgtgcacc gggtcacagg aaggtatcag cttcgccaca agagcgtggc
     4021 cggtttcatg gaacgcgatg atcttctttt ccttttcgct cacgaggcgg cttttccgtt
     4081 cgggaccggc gatgaccctg tcgattccct cctcgaaatt tctcatggaa atttcctttt
     4141 ccccgtttcg cgctgagagg agggccgctt cgttcacgag gttcgccagg tcggcgccca
     4201 caaaacccgg ggtccggcgg gcaagaactt caaggtcaac atccaaagcg agcttctttt
     4261 cccggacatg aacctgcagt attgcctccc ggcctttcac gtcaggcctg tcaacgacta
     4321 tgtgcctgtc gaatctgccg ggacggagca gtgcgggatc caggatatcc ggcctgttcg
     4381 tggccgcgat cagaattatg cccgtggatt catcgaatcc gtcgagttcc acgagaagct
     4441 ggttcagcgt ctgttcccgt tcgtcgtgtc ctcctccgag accggctccg cgctgtcttc
     4501 cgacggcgtc catctcgtcg atgaaaataa tgcacggctg atatttccgg gcctgctcaa
     4561 acaggtcccg aactcttgcc gcgcccacgc cgacgaacat ctcgacgaag tcagaaccgc
     4621 tgacgctgaa aaaaggaaca tcagcctctc ctgccgcagc ccgtgcaagg agtgtttttc
     4681 cggttcccgg aggtccgagg agaagaatcc ccctcggtac ctttgcgccg agggcagtga
     4741 acctgctggg atctttcaaa tagtacacta cttccgagag ttcttccttg gactcttcgc
     4801 agccggccac gtcgttgaag gtcaccttcg ggcggttgtc gaggaagagc ttggccttgc
     4861 ttttcgcgaa gttcatcacc ttgcccccgc cgccctgcat gttgtaaagg aagaatatcc
     4921 agacacctat aagcagcagg gtcggaaaca aggaagaaag catgttggcc caccagggag
     4981 ttttctgcgg aggctcaacc tccacattca ctcctttctg ggcgacctcc ttggccaggt
     5041 caccgactcc gaccacgtac gaaacgaatt ctctcccgtc ggtgaacttg cccttgatgg
     5101 aattttccct gacggtgaca gtattgattc tccccgagga aacttccgaa aggaatgtgc
     5161 tgtacccgat ctcctgtatt tgctggggac cctgtgtggg agaaagaaaa atgttcacga
     5221 ggctcacaac gagcacaatg agaataagat acaacccgag attttttacc aatcggccca
     5281 aattatcgcc gcctcctgac taggtttgaa ttataatgcc gctctcgatt ctcctgaaac
     5341 atcaacaata tgaacggagg ggagattcct ccatttgccc gcataatcaa ggccgtagcc
     5401 tacaacaaat ctgtcgggaa tggaaaaccc cttgtaggca acatccacat gaattttccg
     5461 ccgttcctcc ttgtcgagaa gggcgcatac ccttacgctt cccggctgcc tctcatgcat
     5521 cagcttgaca agatatgaca gagtcagccc cgtgtcaact atgtcctcga cgataaggac
     5581 atttttgtct ttgatgctgc tgtcgagatc tttcacgatc ctgactatcc cgctggtttt
     5641 cgttgaatcg ccataggagg ataccgccat aaaatcaaga gaaatgttca ctgacggcgg
     5701 gatgttccgg accaggtcgg ccatgaacac gaccgcacct ttcagaatgc cgacaacaac
     5761 gagctcctgc ccttcgtagt ctgcagcgat ccgtgctccc agttcggcaa ctcgtttttc
     5821 aatttcctct ttcggtatca ggacatccga aaccttatac tccatagtct ttcccctttt
     5881 cagcagttaa gtggtaaacc tcggcctgaa tgactgcgac ctgatctccg gtttcattta
     5941 atattccgga ggtccgtctc ccccaaaacg gaatccaatc gaaggaaccg gagcggagta
     6001 caggaataat tcgccttgcc caacggggag cccaggcgtc tttgtactgc tgcaattccg
     6061 aaagggggga gacgtgaatt ataccggacc agggaaggac cgcccgcatc catccggcgc
     6121 ataccttctc cgaggtctct ctcttccatg taaactgcca gccgttccat tcgaaattac
     6181 cttggttgcc ttcaagatgg acattcaatc ccgagggatc tggttgatta agtataacag
     6241 ggtcgaccca ggtaacatag gggaaactgc agaaaacata catgctgctc tgccattgga
     6301 agcaccaggg accgtctgtc ctgagaagtc cgacgagagt ttccgtcctt tccctggaga
     6361 gggctttcag gccgagaatc cggcctgctc ctcgaagaaa aacggccgcc gtttccccgt
     6421 caagctttct aaggaacgag accgggcagg cataagaaca aaagggcagc gataccgagg
     6481 cgagacggag gagggacttc tgcagctttt cctcccggcc gcggaagaat gcgagatcgc
     6541 ccgccgtacc gagaatgtgt ttcctggcgg aactgttgac ttctctttca ataaacggaa
     6601 tgagaacggt cctgattttg tttctcaggt aggccgtgtc ctcgttggtg gaatcctccc
     6661 gccagggaag gccataccag cggagaagct gacggaggta atccctggaa tattgcagga
     6721 gaggacggaa aatgaggccc cgtttctcag ggatgcccgt aagtccccga acgcctgttc
     6781 cacgaaacag gttgtgcaga aaggtttccg ctgagtcgtc tttcgtgtga gcgaccccaa
     6841 tgccccatgc gccgagagcc tttccggttt cttccaggaa acggtatctt attcttcgcg
     6901 cgccgtcttc gagggattct cctttttgga gaagggacgg aacaggaaga gattcactga
     6961 tgtacctgac cccgaagcgg gctgccattg aagccacgaa ccgggagtct tccttccctt
     7021 cttctccgcg aatcccgtgg tcaagatgag cgaccaccag gtttgtattc cggaacctgg
     7081 agaaaagcca gagcatggcc atggaatcgc ttccgcccga aacggccaga aggattgggg
     7141 attccgtatg cagccagccc tgttcgatac cggtttgggt gaattctttt ttcaggcggg
     7201 caagagaata atcgaatggg atgtcgggac ggggagattt ctccatcgta tccatgatca
     7261 cctcccgaaa agaaaaaggc ggagaacaat ctccgccttc atggtattct caattggtgg
     7321 cggtgactgg aatcgaacca gtgacactgc gggtatgaac cgcatgctc
//
LOCUS       DAOTHD010000007         7143 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_12525_length_7143_cov_3.589870, whole genome shotgun sequence.
ACCESSION   DAOTHD010000007 DAOTHD010000000
VERSION     DAOTHD010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 7143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7143
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12525_length_7143_cov_3.589870"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    223..232
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<233..1161)
                     /locus_tag="P5219_00260"
     CDS             complement(<233..1161)
                     /locus_tag="P5219_00260"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HRX25216.1"
                     /translation="MAGFVEALTPREKALSSAAAAFGFPWRGGKLSVPADSCSGRILV
                     PVTAGENYPPFSRSTRDGYAVSSADCLGAASGSPAYLTLAGEIPMGAAPSFSLAPGQC
                     ALIHTGGILPEGADSVVMVEDTESAGNWIEVKMAVQKAENTIRRGEEFSLGDTMLKAG
                     QKVDFRTAGLFAMAGIRHAVIARLAVGIISTGDEIAPPDTVRLPPGKVRDVNASMLSA
                     LLAGEGHTVQNYGIAEDTRDSLAASLREALRENDVVVISGGSSVSTRDHCSSILEDLP
                     HPGLLVRGILMSPGKPTLIAGMKEDRQLVLGLPG"
     gene            complement(1191..1865)
                     /locus_tag="P5219_00265"
     CDS             complement(1191..1865)
                     /locus_tag="P5219_00265"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRX25217.1"
                     /translation="MSTRESRRLKEKRSMIPFGDIMLPVIGLVAVGLLIVGVKLFFLS
                     GPKTPGYLPISPSVPSRQQEARPAAVPSDTRETPSEDTPPSAPENRPLLAAPVGGSRP
                     VSAVPAPSGAKSSPVAAPPAAQPVKKASPPSQARPAVQSPAPAAANSRWGVQIGSFTA
                     RDGAETVRQQAAKAGFSSVISSALVNRKTYYRVTVPAGNNRTQADDLAERLKKAGFPV
                     FVVGMR"
     gene            complement(2051..3202)
                     /gene="ftsZ"
                     /locus_tag="P5219_00270"
     CDS             complement(2051..3202)
                     /gene="ftsZ"
                     /locus_tag="P5219_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRX25218.1"
                     /translation="MEHVFQVNDTPRPHREEIKVIGVGGGGGNALNHIIRSGVSGVHF
                     ISANTDVGALQLSQATTKITLGEKLTKGLGAGSDPEVGMNSAKESIDKIREVVTGADM
                     VFLTAGMGGGTGTGASPIIAEVAKEAGALVVAVVTFPFSFEMNRRRKQAMTGIATLKE
                     KVDALLVVENDRLLELTDEKTKHADAYRLADEVLRQAVQGVTDLILKPSLINVDFADV
                     RTVMQNAGSAIMGIGSGEGENRAEAAAKTAIKSPLMSIPMKGAKGILFNITGGSDLGI
                     HEIRKAAQIITETADEDAIVIWGHTVSEEMSDKIQITVIATGFSSEREKKTVTNFTKA
                     APKAKTAGVVLEETEVLPAQDEDLFTMSGVPKNPYDIPSILRRSQKNGK"
     gene            complement(3235..4551)
                     /gene="ftsA"
                     /locus_tag="P5219_00275"
     CDS             complement(3235..4551)
                     /gene="ftsA"
                     /locus_tag="P5219_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRX25219.1"
                     /translation="MYKEPEILVGLDLGTSKIAVVVAERDSRFQEAQIIGVGFAPSQG
                     IRKGLIINLEQAVRSVRQAVKDAENMVGFEITEATVSFSGIDVASVTSRGMISLGRTP
                     RQVSISDVERVIEAAQSELSIPSNRVTLHTIPVKYSIDGNSGIDDPLGMTGIRLEMEL
                     QSVIIPTTVVQNIINCVETAGVEVNGLVIKSLASALGALTDEETRAGVISLCIGGGTT
                     GVALYTDGRPVKLSIIPIGGDHITNDLAYVMKIPISKAEEIKKMVSLAPDDDEEQELD
                     VDVRGRTKTFNIHEASEVVNCRLEELFGEHVLHQLSEYTLHMFPAGVVLSGGVAKTPG
                     IELFASELLNLPVRIAEPLDMYQMPPGRNDCEYTTAAGIVRYMTSKERNPYRFIETPV
                     SQLRSGTSIISPGLPGTKPPRKQGNSHGEGFRGLMEYLKKSVKELF"
     gene            complement(4552..5355)
                     /locus_tag="P5219_00280"
     CDS             complement(4552..5355)
                     /locus_tag="P5219_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25220.1"
                     /translation="MGKFLFFLLACSLFLGSLFSYEGYTQFLSLDGISVRTGDPEAEA
                     FFWKNLGPEELRYWPVFLFRSADLKKKIESESPLLISLRRENLSDFFIEIEPLQPWLT
                     LRWKKTEFFLTRDGRIWESGHPLNTRLAGIRPPSTPTFLLSDSLPSPAGIPEEGIVVM
                     NSVFPVTFFSEWVSGIETSGWMPHTRSVEVSRREGNYLLELNLNVRDRTVHILLRGDQ
                     ARWKEIASAVSQILHQLQFSGGNLIIDTTYTDRIIVRNVAGGGQEGSGR"
     gene            complement(5428..6801)
                     /gene="murC"
                     /locus_tag="P5219_00285"
     CDS             complement(5428..6801)
                     /gene="murC"
                     /locus_tag="P5219_00285"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRX25221.1"
                     /translation="MGIGGAGMSGLALLLAELGYEVSGCDMSHTSYVDKVLKEEIAVV
                     LGHGKAHMDKFLPELLVYSSAIPEENEEIAAAKERGIAVAKRGEVLSWIFDSRFGIGV
                     AGTHGKTTTSSMIALILEQAGLHPTLAIGGELCDIGVNAKLGDGPYMVAELDESDGSF
                     EKFHPNIAVVTNADWDHVNHYPTFESVLEAFTRFTSNPKDGGLLVLCGEDAGLGKIMA
                     AGSLPREAVTYGWGMGWTWGAFNVKHNHGGGVSFSIAHKGVHIDDMELSVSGDHNILN
                     ALAACAVAHTLGIPFTMIKKTLKEFKGAKRRLQHLGQINGVEVYDDYGHHPREIAATL
                     SAIDRMFPEKRLLVAFQPHRFTRTLALYRDFAAVLSSADSVVLLPVYQADEEPIEGVS
                     SALIDALLNENGYSKSIMCGSLEEAASELEKQCLPGDIVLTIGAGDISNVGEMFMEKA
                     RVEVVHA"
     gene            complement(6842..>7143)
                     /locus_tag="P5219_00290"
     CDS             complement(6842..>7143)
                     /locus_tag="P5219_00290"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRX25222.1"
                     /translation="ARGGASTLSELMLWGIPAVIVPWKQAADNHQQRNAECFEKIASG
                     EIWREDEPLEFLKEKILRVLRKAQTVRKEFSEGDESERLWRLISSSIGREITNLG"
BASE COUNT         1545 a         1946 c         1903 g         1739 t
ORIGIN      
        1 tcggccagag cttttgaaaa tgaggatttg acgggaagag ggaaagctct gccgtttctc
       61 agaacgcagg ggataaattc ttcgatgcct gtatggccga ataccggttc ctgcaccggg
      121 acgaaaaccc gcttccatgg tccttccgtt tttccccaga gaagaccgtt cagaagaggg
      181 agaagaacag tatatgccga aatgaaacat gaaaaaggat gtnnnnnnnn nncccggaag
      241 accgagaacg agttgcctgt cttccttcat tcccgcgatc agggtcggtt tgccgggaga
      301 catgaggatg cccctcacca ggagcccggg atggggaagg tcttcgagaa tggatgagca
      361 gtggtctctc gtgctgacgg aggatcctcc gcttatgacg accacgtcgt tttctctgag
      421 cgcttccctg agcgatgcag cgagagagtc tctggtgtct tcagctattc cgtaattctg
      481 aacggtatgg ccttctccgg cgaggagagc cgagagcatg gaggcattta cgtctcttac
      541 cttccctgga ggaagccgga ctgtgtcagg aggtgctatt tcatcccctg tactgattat
      601 gccgaccgcc aaccgggcaa tgacggcatg cctgatgccg gccatcgcaa aaagaccggc
      661 tgttctgaag tcaaccttct gcccggcctt gagcatggta tctccaaggg aaaactcttc
      721 accccgccgt atcgtatttt ccgccttttg aacggccatt tttacttcga tccagtttcc
      781 ggcgctttct gtatcctcca ccatcacaac ggagtctgcc ccttcgggaa ggatgcctcc
      841 ggtatggata agggcgcact gtcccggtgc tagggaaaag gacggggccg cccccatggg
      901 aatttctccc gcgagggtaa gatacgcggg gctgccggaa gcggcgccga ggcaatcagc
      961 gctggatacg gcatacccgt cccgtgtgct tcgggaaaag ggaggataat tttcaccggc
     1021 agtcacaggc acgagaatcc ttccggagca ggaatccgcc ggaacggaca attttcctcc
     1081 ccgccatgga aaaccgaagg cggccgctgc agaagaaagg gctttttctc ttggagtgag
     1141 ggcttcgaca aaacctgcca atagtatgaa cgctccttct gaattcggta tcagcgcatg
     1201 ccgacgacga acaccgggaa tcctgctttt ttcagtcttt cggcaaggtc atccgcttgc
     1261 gttctgttgt ttcctgcagg gacggtgacc ctgtagtacg ttttcctgtt gacaagagct
     1321 gatgatatga ctgatgaaaa accagccttg gcggcctgct gcctgaccgt ttcagctcca
     1381 tcccgcgcgg tgaatgagcc gatctggact ccccatctgc tgttggcggc cgcaggtgcg
     1441 ggactctgaa cggcaggcct ggcctgggaa gggggggaag ctttcttgac aggctgggct
     1501 gcaggaggag ccgccaccgg cgaacttttc gctccggaag gggccggaac agcagataca
     1561 ggccggctcc cacctaccgg agccgcgaga agcggccggt tttccggagc agacggagga
     1621 gtatcctcag acggtgtttc ccttgtgtct gacggcacgg cggcaggacg cgcttcctgc
     1681 tgccgtgacg gtactgaggg tgaaatgggc aaatacccgg gagttttcgg tcccgacagg
     1741 aaaaaaagtt tcactccgac tatcaggagt ccgacagcca ccaggccgat gaccgggagc
     1801 atgatatccc cgaaaggaat catggagcgt ttttccttga gtcttctgct ctctctcgtg
     1861 gacattcaaa tccctccttt gcctgtaaag cggcaggaac agaatcagcc ccatcttctc
     1921 tgttcccgaa aaacaacata tccgaaacaa aaggcaggaa tcctggtcaa gatccctgcc
     1981 ttcagcattt tcgaccgaac agaaaatccg gcccgaggaa tctattgaaa ggattgtacg
     2041 ggaaagatat ttatttcccg ttcttctggc ttctgcggag aatggacggg atatcgtaag
     2101 gatttttcgg cactccgctc atggtgaaca aatcctcatc ctgtgcggga agaacttctg
     2161 tttcttccag caccactccc gcagttttgg cctttggtgc cgccttggta aaattggtga
     2221 ccgtcttttt ttccctttcc gaggaaaacc cggtggcaat gactgttatc tgaatcttgt
     2281 ccgacatttc ttccgagacg gtatgtcccc aaatgacgat ggcatcttcg tccgcagttt
     2341 cggtgatgat ctgtgcggcc ttccggattt catgaatgcc gaggtccgat cctccagtaa
     2401 tgttgaagag tattcccttg gcacccttca tcgggatgga catgagaggc gatttgatcg
     2461 ccgtcttggc ggccgcttcc gcccggtttt cgccttctcc ggagccgatt cccatgattg
     2521 cggaacctgc gttctgcatt accgttctca cgtcagcaaa gtcgacgttg atcaggctgg
     2581 gtttcagaat aaggtcggtc actccctgaa ctgcctggcg cagaacttcg tctgcaaggc
     2641 gataggcatc ggcgtgtttt gttttttcat cagtcagttc aaggaggcgg tcattttcaa
     2701 ccacgagaag ggcgtccact ttttccttga gtgttgctat tcccgtcatg gcctgctttc
     2761 tccggcggtt catctcgaag gaaaagggaa aggtaaccac cgcaacgacg agggctcccg
     2821 cttccttcgc aacttccgcg attatgggcg atgcccctgt tccggtaccg cctcccatgc
     2881 ctgcggtgag aaagaccatg tcggcccccg tgacgacttc ccgtatcttg tcgatcgatt
     2941 cctttgcgct gttcatgccg acttcgggat cggaacccgc tccaagccct ttggtcagtt
     3001 tttctccgag ggttatcttc gtcgtggcct gagagagctg aagagcaccc acgtccgtat
     3061 tggctgagat aaagtgaact cctgacacac cgctcctgat gatatggttc agggcgtttc
     3121 cgccgccgcc gccgacgccg atgaccttga tttcctcccg gtggggcctc ggagtgtcat
     3181 taacctgaaa tacatgctcc attttcttgt cctccccaat tgcggaatgc ggctctagaa
     3241 aagttccttg acggattttt taaggtattc catgagacca cgaaacccct ctccatggga
     3301 attcccctgt tttctgggag gttttgtccc gggaagtccc ggcgaaatga tggatgtgcc
     3361 ggaacggagc tgggacacgg gggtctcaat gaaacggtac ggatttcttt ccttgctggt
     3421 catataccgc acaattcctg cggcagtagt atattcgcag tcatttcttc ccggaggcat
     3481 ctggtacatg tcaaggggtt ccgcgatgcg gacgggaagg ttgagaagct cggaagcgaa
     3541 aagctcgatt cccggtgtct tggcgacacc tcctgaaagg acgacgcctg cggggaacat
     3601 gtgcagggtg tattcggaaa gctgatggag cacgtgttct ccgaacagct cttcgagccg
     3661 gcagttcact acttccgagg cttcatgaat gttgaaggtt tttgttcttc cccttacgtc
     3721 gacgtcaagc tcctgttcct cgtcatcatc cggggcgaga gagaccattt tctttatttc
     3781 ctctgccttg cttataggaa ttttcatgac gtatgcaagg tcgttggtta tgtgatctcc
     3841 tccgatggga atgatggaaa gcttgacggg acgcccgtcg gtgtaaagag cgacacccgt
     3901 cgtccctccg ccgatgcaaa gggaaatgac tcctgctctc gtttcttcgt ctgtcagcgc
     3961 accaagggcg ctcgcgagcg atttgattac aagtccgttc acttccactc cggcggtttc
     4021 aacacagttg atgatgttct gaaccacagt tgtggggata ataacggact gaagctccat
     4081 ttcgagcctg atgccagtca tccccagagg atcgtcaatt cccgaattgc cgtcgatgga
     4141 atatttaacc ggtatggtat gaagggtaac cctgtttgaa ggaatggaca gctcactttg
     4201 ggccgcctcg atgactcttt ccacgtccga gatggaaacc tggcggggtg ttctgcccag
     4261 ggaaatcatc ccgcgggacg ttacggaggc aacatcgatg ccgctgaaag agacggtagc
     4321 ttccgttatt tcgaacccca ccatgttttc agcgtctttg accgcttggc gtacagagcg
     4381 gaccgcctgc tccaggttta tgatcaggcc ttttcgtatt ccctgggagg gagcgaaccc
     4441 gacaccgatt atttgagctt cctggaaacg ggaatctctt tccgccacta caacggcgat
     4501 tttgctcgtg ccgagatcca gcccaacgag aatttccggc tctttgtaca tttatctccc
     4561 gctcccttcc tgaccgccgc cggccacatt acggacaatg attctgtcgg tgtacgtggt
     4621 atctattata aggtttccac cagaaaattg taactggtga agaatctgag agacggcgga
     4681 ggctatttct ttccaccgtg cctggtcacc ccgaaggagg atatgcacgg tcctgtccct
     4741 gacattcaga ttcagctcta gcaggtagtt tccttccctt ctggaaactt ccacgctacg
     4801 agtatgaggc atccagccgc ttgtttcaat accactgacc cattcagaaa agaacgtgac
     4861 aggaaaaacg ctgttcatca ccacgattcc ttcttccgga atgcctgccg gtgacggaag
     4921 gctgtcggaa agcagaaagg tcggagtgga aggcggccgg attcccgcaa gccgtgtatt
     4981 gagggggtgg cctgattccc atatgcgtcc gtcacgggtg agaaaaaatt ctgtcttttt
     5041 ccaccgcagt gtgagccagg gctgtaaagg ttctatttcg ataaagaaat ccgataaatt
     5101 ctcccgtctc agagaaatga gaagaggaga ttccgactcg atttttttct tcaggtccgc
     5161 cgacctgaaa agaaaaaccg gccagtaacg caactcttcg gggccaaggt ttttccagaa
     5221 aaatgcttcc gcctcgggat cccctgttcg aaccgaaata ccgtcaaggc tcagaaactg
     5281 ggtatacccc tcgtaggaga atagtgaacc gaggaacagg gaacacgcaa gaagaaaaaa
     5341 aagaaatttt cccatggaat cgctaccccg gcaacggagg gaaaacaccg gcacctgaag
     5401 gccgtccggc atcccctttc ataccggtca ggcgtggaca acctccactc ttgccttttc
     5461 catgaacatt tccccgacat tggaaatatc tccggcaccg attgtcaaaa cgatatcccc
     5521 cggcaggcat tgtttctcga gctccgaggc cgcttcttca agagagccgc acataatgct
     5581 ctttgaatat ccgttttcgt tcaggagagc atcgatgagt gcggaggaaa ccccttcgat
     5641 cggttcttcg tccgcctggt acacggggag aagcacaacg gaatccgccg aagaaagaac
     5701 cgctgcaaaa tccctgtaca gagcaagcgt tctggtgaac ctgtgaggct gaaaagcgac
     5761 gagaagccgt ttttcaggga acatcctgtc gatggcgctc agggttgccg cgatctccct
     5821 gggatggtgt ccgtaatcgt cataaacctc gactccgttg atctgtccca ggtgctgaag
     5881 gcgtcttttg gcgcccttga attctttcag ggttttcttg atcatggtga agggaatccc
     5941 cagagtgtgg gctacggcac aggcggccag ggcattgaga atattgtggt ctccggaaac
     6001 ggagagttcc atgtcgtcaa tatggacccc cttgtgggcg atggagaaac tgactcctcc
     6061 tccatgattg tgcttcacgt tgaaggcccc ccatgtccat cccatccccc agccgtaggt
     6121 taccgcctcc ctggggagcg atcctgcagc catgattttg ccgaggccgg cgtcttctcc
     6181 gcagagcacg agaagacctc cgtccttcgg attggatgta aatcgtgtga aggcctcaag
     6241 aacgctttca aatgtggggt aatgattgac atggtcccag tcggcgttgg tgaccactgc
     6301 aatgttggga tgaaattttt cgaaggagcc gtcgctctcg tcgagttcag caaccatgta
     6361 tgggccgtca ccgagtttcg cgtttacgcc gatgtcgcac agttctcccc caatggcgag
     6421 ggtcggatga agcccggcct gttccaggat gagagctatc atggaggatg tggttgtttt
     6481 cccgtgggtt ccggcaacgc ctatgccgaa ccgggaatcg aagatccagc tcaggacctc
     6541 tccccttttg gctactgcaa ttccgcgctc ctttgccgcg gctatctcct cgttttcctc
     6601 ggggatggcg ctgctgtaaa cgagaagctc aggaaggaat ttatccatat gggcctttcc
     6661 atgcccgagc acaacggcga tttcttcttt caacaccttg tcgacatagg aggtgtggct
     6721 catatcgcag ccgctgacct cgtagccaag ttctgcgaga agaagagcaa gtccgctcat
     6781 ccctgcgcct ccgatgccca taagatgaat ccggcgtata cccttcaagc ttgcgatgta
     6841 attatccaag attcgtaatc tcccttccaa tactggacga gatcagccgc cataaacgtt
     6901 cagactcatc tccctccgaa aattctttcc tgacggtttg tgcctttcga aggacgcgca
     6961 gtatcttttc tttcaagaat tccagaggtt catcttctct ccagatttct ccggaggcaa
     7021 ttttttcaaa acattcggcg tttctttgct ggtggttgtc cgcagcctgc ttccagggaa
     7081 caataacggc agggatcccc caaagcatca gctcggaaag cgtcgatgcc cctccccgag
     7141 cta
//
LOCUS       DAOTHD010000008         7102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_12611_length_7102_cov_3.205761, whole genome shotgun sequence.
ACCESSION   DAOTHD010000008 DAOTHD010000000
VERSION     DAOTHD010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 7102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7102
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12611_length_7102_cov_3.205761"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..282)
                     /gene="fliQ"
                     /locus_tag="P5219_00295"
     CDS             complement(28..282)
                     /gene="fliQ"
                     /locus_tag="P5219_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HRX25223.1"
                     /translation="MYDVLSDAVWTTLITSLPVLLVAMLVGLVIGILQTATSIQEQTL
                     IFIPKIVAVLLALVAFGPWMFGRVGDMARLIFGQLERFVR"
     gene            complement(312..1031)
                     /gene="fliP"
                     /locus_tag="P5219_00300"
     CDS             complement(312..1031)
                     /gene="fliP"
                     /locus_tag="P5219_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806178.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HRX25224.1"
                     /translation="MVTTSAFAQDGAPFIPLPALQFGVRMAESPSDVAVTLQILALLT
                     VLSLAPAIILMVTSFTRILVVLGFVQRAIGLQQTPPNQVIVGLALFLTLFVMGPTWGK
                     VYDGALSPYLAGNISSAQAWEGTIQPVREFLFKFTRQEELSLMVSMAGMEQPRNRDDI
                     PTRILLPAFMLSELKTAFQMGVVIFIPFIVVDMIVASVLMAMGMIMLPPMMISLPFKV
                     LLFVMADGWNLVVTSLLKSFT"
     gene            complement(1430..1792)
                     /locus_tag="P5219_00305"
     CDS             complement(1430..1792)
                     /locus_tag="P5219_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX25225.1"
                     /translation="MGRKVLIVDDAAFMRMMLKDILTKNDFEVVAEAENGKVAVASFQ
                     KFKPDIVTMDITMPEMNGIDAVKAIKSIDGSARIVMVSAMGQQPMVIEAIQAGATDFI
                     VKPFQPDRVVEALNKALG"
     gene            complement(1840..2988)
                     /gene="fliY"
                     /locus_tag="P5219_00310"
     CDS             complement(1840..2988)
                     /gene="fliY"
                     /locus_tag="P5219_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch phosphatase FliY"
                     /protein_id="HRX25226.1"
                     /translation="MIDELLSQDEINALLSGADFGGDGGAGKSEGGISPDQQSILDDL
                     AGIFSTSASSVYGMLAGKEVSALVREMTVLPQKEAASRPEETPFAFRVTCGGLNDAPL
                     VLVITERGALTLADLMMGGEGKDLPEEAGELYLNAAQEGLSQVVGAAFTGMSGMLGGK
                     RLIPENISSALESQDWLPFPTRSPEDEAWTISAAISIEGLGDFPLWVILPLDVAKALA
                     DDVRELVSGKGKKEAPAAARAPAAGAKPQQPSSRGNVPVEAPVQETEHPSFGNVYDQS
                     SVDVRPAEFVPLVQKAGTGYGNSRIDLVADIPVRVTVELGRTRKNISDILNMTPGSVI
                     ELDKMAGEPVDILVNGKAVAKGEVVVIDENFGVRITEIVSSAGRVHSL"
     gene            complement(2981..4000)
                     /gene="fliM"
                     /locus_tag="P5219_00315"
     CDS             complement(2981..4000)
                     /gene="fliM"
                     /locus_tag="P5219_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HRX25227.1"
                     /translation="MTPDVLSQSEIDSLLEALSSGSVDVDAMRKEDEKKIKLYDFRRP
                     DKFSKDQLRAIQMIHESFTRQLTTTMSTMVRSMVSAEIASVDQFAYDEFVRSLVQPTV
                     IGILEMYPFSGNALIEINPNLVFAIIDRMLGGKGEFSGKVRELTDIERTVVERVIMRM
                     LELLEESWSTVVDVRFRYENLESNPFFVQICPGTDMVLLVILKLKVGDVEGMVSICIP
                     YFLMEPIMDKLSSQQWFASTGRKSDEGSRDYILKHMQNVRVPLALELGHTVLNVADVM
                     QLRTGDVIKLDETLKDPLSVRIGNLVKFRAVPGSVNGRYAAEISEIIPGEDELPEREG
                     GAVND"
     gene            complement(4046..4492)
                     /locus_tag="P5219_00320"
     CDS             complement(4046..4492)
                     /locus_tag="P5219_00320"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HRX25228.1"
                     /translation="MKRILLFLILAILLLSLGFGGGVFAGRMWLSPEGGSSGTEIEEP
                     GPVFFVGDFISNLAGAGNHVANFKLSMEMSGPKALEMISSPNWLARVKNEVILLTKDR
                     VFEDLTSAEGILALAEDIKRTVNAIMPPVRDKVPVVRVLFEGFVLQ"
     gene            complement(4532..5254)
                     /locus_tag="P5219_00325"
     CDS             complement(4532..5254)
                     /locus_tag="P5219_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HRX25229.1"
                     /translation="MARKRKEESSPGAPLWLVTYGDMVTLVLTFFVLLFSFSSIDVQK
                     FEMMIMSFQGALGFMPGGKSVQQDPAVFGGQPATQAGESRRVTPDIQQVAQNLRNYLR
                     SEGLDKEVVVRIDQRGVTVSLSDQFLFDSGKAELKPEGMRVLFKIAEFIKDSVPAVAV
                     EGHTDSVPLKGGIYRDNWGLSSARAAAVASYLQFRGNFDPLKLQAVGYGPNRPLVPND
                     TPEHMALNRRVDLVFLSRHPKQ"
     gene            complement(5254..6045)
                     /locus_tag="P5219_00330"
     CDS             complement(5254..6045)
                     /locus_tag="P5219_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HRX25230.1"
                     /translation="MDLTTVIGLAVCFILVIGGVVAGGEGIAFVDFQSMLIVLGGTGG
                     ALIMSNPPERIKSFLKIMKMAFTGGSPDLVSLVQTIVSFAEKARREGLLALEADASEL
                     DNEFLKKSIQLVVDGTDPELVKAILDTEIGILENRHSSNKYFFDSMAELAPAFGMLGT
                     LIGLITMLGNLSNPDALGPGMAVALVTTFYGSLIANGFALPIGKKLAVRSSQEVLSME
                     LMVEGVLAIQAGENPRIVEEKLKVFLPPKQRLDLEEKAAEAKGGA"
     gene            complement(6096..6326)
                     /locus_tag="P5219_00335"
     CDS             complement(6096..6326)
                     /locus_tag="P5219_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HRX25231.1"
                     /translation="MITLRRINGEAFTINADLIETVESTPDTVIRLVNGHRYVVAEPM
                     EEIVDLVVKYRQKVLFSFFGGKNLLSDEEEKS"
     gene            complement(6342..>7102)
                     /locus_tag="P5219_00340"
     CDS             complement(6342..>7102)
                     /locus_tag="P5219_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HRX25232.1"
                     /translation="LLNGSTSLATINQKIASVHNTKLDVYDSLGNAHTLEVSWEKIDN
                     NQWRWRAWLPSEPGVTISNNTGIMEFDSDGKITTGSSGVIGLNFASLGADDATITLDF
                     TGESFGKDVMEGVTQYGSTFTTKGYYQDGYGMGVLNDFAAGKDGTITGVYSNGVNLPL
                     YRIALALFSNPTGLTKVGSTAFAASNNSGLAQVVAAGEGGAGTIAGGTLEMANVDLSE
                     EFVRLIVAQRGFQANARVVTTSDQVLEEVINIKR"
BASE COUNT         1421 a         2128 c         2063 g         1490 t
ORIGIN      
        1 gggtcacaaa gcgggtctgt tccgccgtca tcggacaaac cgctcaagct ggccgaatat
       61 gagccgcgcc atgtcgccga ccctgccgaa catccagggg ccgaaggcca ccagggcgag
      121 gaggacggcc acgattttcg gtatgaagat aagggtctgc tcctggatgg aggtggccgt
      181 ctggagaatg ccgatcacga gacctacgag catggccacg aggagcaccg ggagggacgt
      241 gatgagcgtg gtccagactg cgtcgctgag cacgtcgtac atgttcacgg gaaacactgg
      301 tcatcgcccc cttacgtaaa gcttttcagc aggctcgtca ccacgaggtt ccagccgtct
      361 gccatgacga agagaagcac cttgaagggc agggagatca tcatgggcgg aagcatgatc
      421 atgcccatgg ccatgagaac gctggccacg atcatgtcca ccacgatgaa ggggatgaag
      481 ataaccaccc ccatctggaa ggcggtcttc agttcgctga gcatgaaggc cggcaggagg
      541 attctcgtgg ggatgtcgtc ccggttcctc ggctgctcca tcccggccat ggataccatg
      601 agagacagct cctcctggcg ggtgaacttg aaaaggaact ccctcacggg ctgtatggtg
      661 ccttcccagg cctgggccga actgatgttt cccgcaaggt agggggagag ggcgccgtcg
      721 tacaccttcc cccatgtggg ccccatgacg aaaagggtaa gaaacagggc cagcccgacg
      781 atcacctggt tcggcggtgt ctgctggagt ccgatggccc gctggacgaa gccgaggacc
      841 acgaggatcc gggtgaagct ggtcaccatg aggatgatgg ccggggccag gctcagtacg
      901 gtcaggagag cgaggatctg gagggtcacc gctacgtcgg atggggattc cgccatgcgg
      961 accccgaact ggagcgcggg cagcggaata aagggagccc cgtcctgggc gaaggcggag
     1021 gtcgtcacca tcgtggcgga caccgcggcc gaaaggaatg gtatccaatt gagaatgagc
     1081 ttcgtcctcg aattgtttcc agtcttcata tcgccacctg ccgatcaccc tcgtcccggc
     1141 gcctcccgat gccagggcga ccacgtccgg cccgagccgg acgataaaaa gaacatcctt
     1201 gccgaggggc aaggatgcca ttatgttcgc ggctcccttc gccttcaggg gagctgtccg
     1261 ccgggaatat aacaccatgc cgtatccggc gagagcaagt atggcaaggc tcagcccgat
     1321 ccggatcatg tacccggcaa gagaggggga atcagccgga gaagcctccg cggcggccgg
     1381 aagaaggacc agaaagaccg cggcgcagaa agcggtcatg gcgaaccttc tagccgagag
     1441 ccttgttgag cgcttccacc accctgtcag gctggaaggg tttcacgatg aaatccgtcg
     1501 ctccggcctg gatagcctcg atgaccatgg gctgctggcc catggcgctc accatgacga
     1561 tcctggcgct cccgtcgata gacttgatgg ccttcaccgc gtcaattccg ttcatctccg
     1621 gcatcgtaat gtccatggtg acgatgtcgg gcttgaactt ctggaaggag gccacggcaa
     1681 cttttccgtt ctccgcctcg gccaccactt caaaatcgtt cttcgtcaga atgtccttca
     1741 gcatcattct catgaaggcg gcatcgtcga ctatcagaac tttcctgccc atacttccac
     1801 atccctctct cggtaatagc ttgaacgtcg gatcccttcc tagagcgaat gaacccttcc
     1861 tgcggacgac acgatttccg tgatgcggac accgaaattt tcgtcgatga caacgacttc
     1921 gcctttggct acagccttgc cgttgaccag aatatccaca ggttcaccgg ccatcttgtc
     1981 taattctatc acggaacccg gtgtcatgtt caagatgtcg gagatatttt ttctcgtccg
     2041 gcccaattcc accgtcaccc ggacagggat gtcggccaca aggtcgatcc tgctgtttcc
     2101 gtatcccgtt ccggccttct ggaccagcgg aacgaactcg gcaggacgga catcaactga
     2161 ggactggtcg tacacgtttc cgaaggaggg atgttcagtc tcctggaccg gcgcttcaac
     2221 cggcacgttc ccgcgggagg agggctgctg cggtttcgcc ccggctgcgg gggccctggc
     2281 ggccgccgga gcttctttct tccctttgcc ggatacaagc tcccgaacat cgtcggccag
     2341 ggctttggca acgtcgaggg gaagaatcac ccagagagga aagtctccaa ggccttcgat
     2401 ggaaatcgcc gccgaaattg tccatgcttc atcttcagga gacctggtgg ggaaaggaag
     2461 ccagtcctgg gattccaggg cggaggaaat attttccggt ataagccgtt ttccaccgag
     2521 catgccgctc atacctgtga aggcagcccc caccacctgg ctcagcccct cctgggccgc
     2581 gttcaggtac aattcccccg cttcctcggg aaggtctttc ccttctccgc ccatcatgag
     2641 gtcggcaagg gtgagggccc cccgttcagt aatcacgaga accaggggag catcgttgag
     2701 cccgccgcag gtcacccgga aggcaaaggg cgtctcttcg ggcctggagg cggcttcctt
     2761 ctggggaagc accgtcattt ccctgacaag agcgctgacc tccttgcctg ccagcatacc
     2821 gtagacactc gacgccgacg tggaaaaaat gcctgccagg tcatcaagaa tgctctgctg
     2881 gtccggggaa ataccccctt cgctttttcc cgctcctccg tctccgccga aatcggctcc
     2941 tgacaagagg gcgtttattt cgtcctggct cagaagttcg tcaatcattg accgcccctc
     3001 cctctctttc gggcagttca tcctcgcccg ggataatttc cgaaatttcc gcggcatatc
     3061 tgccgtttac cgaacccggt acggccctga acttgacgag gttgccgata cggacgctca
     3121 gaggatcttt cagggtctcg tcgagcttga tcacgtcgcc cgtcctgagc tgcattacgt
     3181 cggccacgtt gagcaccgta tgccccagtt ccagggcaag ggggacgcgg acgttctgca
     3241 tgtgcttcag gatatagtcc cgggatcctt cgtcgctctt ccttcccgtg gatgcgaacc
     3301 actgctggga gctcagcttg tccatgatgg gctccataag gaaatagggg atgcagatgc
     3361 tcaccattcc ttccacgtcg cccaccttca gcttcagtat gaccaggagc accatatcgg
     3421 ttcccggaca gatctggacg aagaaggggt tgctttcaag gttttcgtag cggaaccgga
     3481 cgtccaccac cgtgctccag ctttcctcga ggagctcgag catcctcatg atgacccgtt
     3541 ccaccaccgt acgctcgatg tcggtcagtt cccggacctt tcccgaaaat tcccccttgc
     3601 cgccgagcat cctgtcgatg atggcgaaca cgaggttcgg gttgatctcg atgagggcgt
     3661 ttccgctgaa ggggtacatc tcgaggatcc cgatcaccgt gggctggaca agggagcgta
     3721 caaattcgtc gtaggcgaac tggtccaccg acgcgatctc cgccgagacc atggaccgga
     3781 ccatggtgga catggtggtg gtcagctgcc gggtgaagga ttcatggatc atctggatgg
     3841 cccggagctg gtccttgctg aatttgtcgg gacggcggaa atcgtagagc ttgattttct
     3901 tctcgtcttc cttccgcatg gcgtccacgt cgacgcttcc gctggaaaga gcttcgagaa
     3961 gagagtctat ttcactttga gagagcacat ccggtgtcac cataatcccc acctccttcc
     4021 cggcgtacgg ctgcccctgt cggggctact gcagaacaaa gccctcaaaa agaaccctga
     4081 ccaccgggac cttgtcccgg acagggggca tgatggcgtt taccgttctc ttgatgtctt
     4141 cggcgagggc aagaatcccc tccgcactgg tcaggtcctc gaaaaccctg tccttggtca
     4201 ggagaatcac ctcgttcttc accctggcga gccagttcgg ggaagagatc atctcaaggg
     4261 ccttcgggcc gctcatttcc atggagagct tgaagttggc cacgtgattc cctgctccgg
     4321 cgagattgga gatgaagtcc cccacgaaaa agaccggacc gggctcttcg atttccgtac
     4381 cggacgatcc gccctccgga ctcagccaca tcctcccggc gaagacgccg ccgccgaacc
     4441 cgagggacag cagaagaata gccaggatca gaaaaagaag tatgcgtttc atgttgtttt
     4501 cccccttgcc ggctgcgcgg acttgagctg attactgttt cggatgccgg gacaggaaca
     4561 cgaggtccac ccgtctgttc agcgccatgt gctcaggcgt gtcgttcggt acgaggggcc
     4621 ggttggggcc gtatccgacg gcctgcagct tcagcgggtc gaaattcccc ctgaactgga
     4681 ggtatgaagc caccgcagcc gcccgggccg atgacagccc ccagttgtcc ctgtatattc
     4741 cccctttgag gggaacggaa tcggtatgtc cctccacggc caccgccggg acggaatcct
     4801 ttatgaactc ggcaatcttg aagagaaccc tcataccctc cggcttcagc tccgcttttc
     4861 cggaatcgaa aaggaactgg tccgaaaggg agacggtaac ccccctctgg tcgatccgca
     4921 caaccacctc tttgtccagc ccctcgctgc ggaggtagtt ccggaggttc tgggcgacct
     4981 gctggatatc cggcgtaacc ctcctggatt cccccgcctg cgtcgcaggc tgtcctccga
     5041 agacggccgg atcctgctgt acggattttc ctcccggcat gaatccgaga gcgccctgga
     5101 aggacatgat catcatttca aacttctgga catcgatgga cgaaaaggag aacagaagca
     5161 caaaaaaggt gaggacgagc gtcaccatgt caccataggt gacaagccac agcggagctc
     5221 cgggagatga ttcctctttc ctcttccgtg ccatcaggct cctcctttcg cctctgccgc
     5281 cttctcctcg aggtcaagac gctgcttcgg gggaaggaag accttcagct tctcctccac
     5341 gatccgcggg ttttcccctg cctggatggc aaggacacct tccaccatga gctccatgga
     5401 gaggacctcc tgggaagagc gcacagccag ctttttccct atgggaaggg caaagccgtt
     5461 ggcaatgagc gacccgtaaa acgtggtgac gagggcgaca gccatgccgg ggccgagggc
     5521 atcaggattc gagaggtttc ccagcatggt gatgagcccg atgagcgtcc ccagcatgcc
     5581 gaaggccggt gcgagctcgg ccatggaatc gaagaagtac ttgttggacg agtggcggtt
     5641 ttccaggata ccgatttccg tgtcgaggat cgccttgacg agttccgggt cggttccgtc
     5701 cacaacgagc tggatggatt tcttcaggaa ttcgttgtcc agttccgagg catccgcctc
     5761 aagggcgaga agcccttccc ggcgcgcctt ctcggcaaaa ctcacgatgg tctgcaccag
     5821 ggagaccagg tccggagatc cgccggtaaa ggccatcttc atgatcttca ggaagctttt
     5881 gatcctctct ggcggattgg acattatcag tgcgccgccc gttcctccga ggacgatgag
     5941 catggactga aagtcgacga aggcgattcc ttcgccgccg gccactacac ctcctatgac
     6001 gaggatgaaa cagacagcca atccgatgac ggttgtaaga tccacgaaaa tcctgcctcc
     6061 cagggtgccg cgtcagttgc cggatcaaaa agggatcagc ttttttcctc ttcgtccgaa
     6121 aggaggttct ttcccccgaa aaaggagaaa agaaccttct gcctgtactt taccactaaa
     6181 tccactattt cttccatcgg ttccgccacc acgtaccggt gaccgttgac gagccggatg
     6241 accgtatcgg gagtggattc gaccgtttct atgaggtcgg cgttgatggt aaaggcctcg
     6301 ccgttgatcc tgcgaagagt gatcatcctc taccgctccg tttaccgctt gatgttgatg
     6361 acttcttcga gcacctgatc gctggtggtg acaaccctgg cgttggcctg gaagcctcgc
     6421 tgggccacga tgaggcggac gaattcttcc gagaggtcca cgttggccat ctccagggta
     6481 cctccggcga tggtgcccgc tccaccctcg ccggctgcga cgacctgggc gagccccgag
     6541 ttgttgctgg cggcgaaggc ggtgctgccc accttcgtca gtcccgtcgg gttggagaag
     6601 agcgccaggg cgatccggta caggggaagg ttcacgccgt tgctgtagac acccgttatg
     6661 gtgccgtcct tgccggcggc gaagtcgttg aggacgccca ttccgtatcc gtcctggtaa
     6721 tatcccttcg tggtgaaggt ggatccgtac tgggtcacgc cttccatgac atccttcccg
     6781 aaggattccc cggtgaagtc gagggtgatg gtggcatcgt cggctccgag ggatgcgaag
     6841 ttcagcccga tgacccccga ggatccggtg gtgatcttgc cgtcggaatc gaattccatg
     6901 atgcccgtat tgtttgaaat ggtcaccccg ggttcggacg gcagccaggc tctccagcgc
     6961 cactggttgt tatcgatctt ttcccagctc acctcgaggg tgtgggcatt gcccaggctg
     7021 tcgtagacgt cgagcttcgt gttgtgaacg ctggcgatct tctggttgat ggtcgccagg
     7081 gacgtgcttc cgttgagaag gg
//
LOCUS       DAOTHD010000009         6615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_13771_length_6615_cov_3.773628, whole genome shotgun sequence.
ACCESSION   DAOTHD010000009 DAOTHD010000000
VERSION     DAOTHD010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13771_length_6615_cov_3.773628"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="P5219_00345"
     CDS             complement(<1..257)
                     /locus_tag="P5219_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1445 domain-containing protein"
                     /protein_id="HRX25233.1"
                     /translation="MKAFDYAGVSPREVRQMIREGKWTLPTPGMCKGHVQGNLVILPK
                     DLAYDFLVFAQRNPKPCPILDVTEPGNPEPKIVAPGADLST"
     gene            complement(254..1027)
                     /locus_tag="P5219_00350"
     CDS             complement(254..1027)
                     /locus_tag="P5219_00350"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HRX25234.1"
                     /translation="MSKIDLNSDLGESFGPWKMGMDAEVMESVCSANVACGFHAGDAE
                     VMIKTVKAAKAAGVAVGAHPGHPDLQGFGRRTMAVTPDEAYAYTLYQIGALRAVCAAQ
                     GVPLEHVKAHGALYNQAAKDLALAKAIARATKDAGGDLILLGLANSLFEQAASEEGVL
                     YAAEAFADRGYMDDGSLVPRSKPGAFVHDLKEAGERMVRLVKEGVIRSAEGKDIHLTA
                     HSICLHGDNPAAVEMARHIRKTLEANGVEVRKIREVLKG"
     gene            complement(1027..2121)
                     /locus_tag="P5219_00355"
     CDS             complement(1027..2121)
                     /locus_tag="P5219_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HRX25235.1"
                     /translation="MSALVLECRLPGMLTTVQDGGRWGYQGKGMPVAGAMDLPSFRRG
                     NILAGNDENDGAIEVTLLGPTLFVAEGEGMVAATGADLGFQINGRPAPMWTAVAVGAG
                     DVLSFSGPLSGCRAYLCFSGGVDVPPVMESRSTYTRARVGGFLGRALKKGDTLRCGAP
                     LSLWRKCEGLVCPEGLRPVYPPEAPLRVVPGPQDDLFTEKGKDTFYGSEYTVSNSADR
                     MGYRMDGPEIEHTGAADIISDAIPLGAVQVPGHGQPIVMLADRQTTGGYTKIGVVCSV
                     DIAALSQRLPGQKVRFQKIPLDDALALAREEAGKRDELRRLRSAWRSAPGEPVPLPVS
                     GPDLPSRGEARIRVDGEEHRISWEEIREVE"
     gene            complement(2118..2867)
                     /gene="pxpB"
                     /locus_tag="P5219_00360"
     CDS             complement(2118..2867)
                     /gene="pxpB"
                     /locus_tag="P5219_00360"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HRX25236.1"
                     /translation="MEEKKYPRILTAGDGCVVAEFGDSIDMEINSRAAALGNEILRLG
                     FPGVLDAVPTYRSLAVYFDPVATDVKRLYSRLEELCGKEESAAGHEKRRTIVVPTLYG
                     GEWGPDLDDVAEHTGFSPEEVVRRHSGLDCYCFMLGFTPGFPYLGGMDPALETPRLKN
                     PRETIPAGAVAIGGKQTGIYSIASPGGWRLIGRTPMRLFDPDRDPPIFLEAGMWIRFR
                     PIEKAEFDEIEAASLKGSYRPVILGEEEAPS"
     gene            complement(2908..4143)
                     /locus_tag="P5219_00365"
     CDS             complement(2908..4143)
                     /locus_tag="P5219_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HRX25237.1"
                     /translation="MTDLGKTQERSRSETLSVLLGAAFLMATSAIGPGFLTQTISFTN
                     ELKATFAFAILLSILIDIVVQLNIWRILAVSGMRAQDVANKVLPGLGYFLAFAVALGG
                     LAFNVGNVGGAALGFEVLFGSEQVVGASISAAIAITIFLWKDLGKAMDKFTQILGFLM
                     LALVFYVVFVTKPPVMETVHEMVKPFTDMNLLKEYKWLVVLTLVGGTVGGYITFSGAH
                     RIIDAGITGKEHLSQISKGSINGIAITGVMRYLLFLAFLGVVLLGTEIDMKNPVASAF
                     QIGAGPLGYRIAGVVLWAAAITSVVGASYTSVSFLRTLFGVVDKYYRFWIIGFIIAST
                     SILTLIGRPVKLLIVAGSLNGLILPLSLGCVLLAAYKKEVVKDYVHPMWMTLLGWVIV
                     VFTAWMGFNSFAGILKLFQ"
     gene            4365..5171
                     /locus_tag="P5219_00370"
     CDS             4365..5171
                     /locus_tag="P5219_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRX25238.1"
                     /translation="MMRRALWLSGGICWFAYVLAPLALILVGSFGEKWFGTLLPDGFT
                     LRWYADLFSKTMYVRAMGMSLLVACLTVFINALVGICSVYAVSVLGKKWLRRAFEFVI
                     LLPIAIPPVVMGLGLVQGFNWPSFSLVGTWQLLLGAHLVYTLPFMLRPLMANMDMLNW
                     TVLEEAGTSLGATPFFLATRVLVPNLVPGLLSGAIMTFAMSLGEFQLAVMVTGSASQT
                     YPVVLYQAFYVSTGFACAATTLLVLFSILSLGAVIFLGRLFGTRSAGMAV"
     gene            5203..5697
                     /locus_tag="P5219_00375"
     CDS             5203..5697
                     /locus_tag="P5219_00375"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HRX25239.1"
                     /translation="MDRSPIISVIGTSTASPGLYELAREVGRLLARAGCTVVCGGRGG
                     VMEAVCRGVSEEGGTSVGLLPGDIREANPYVTIPLSTGLGEVRNFAVASAGEAVISIG
                     GAFGTLSEIGFALRSGKPVIALKSWHISEGGESPSPMIEAATAEEAVRLALEKTGRES
                     HDRG"
     gene            5684..>6615
                     /locus_tag="P5219_00380"
     CDS             5684..>6615
                     /locus_tag="P5219_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX25240.1"
                     /translation="MTGVELRSVSKRFGKTYAVRNVSLSVGKGEFLSLVGPSGCGKTT
                     TLRLVAGFLAPDEGEVLLNGESMGGVGVRQRQVGIVFQNYALFPNLTVFENVAFGLRT
                     RNTPEDVLRPKVEQLLSMVGLDRRASAWPRELSGGQQQRVALARALAITPKVLLLDEP
                     LSALDAKVRNSLRFEIKRIQRESGITTIYVTHDQEEALSISDRVALMNEGRIEQTGSP
                     RDIYLRPANSFVADFVGVNNLLEGEYLGDGRFRWRERILSVENVPLPQGPCSLMIRPE
                     RISIASRDSASGDGNILPGRVSGKVFLGPLLRLAV"
BASE COUNT         1227 a         2061 c         2024 g         1303 t
ORIGIN      
        1 tccgtggaga ggtcggcgcc gggagccacg atcttcggct ccggatttcc cggctcggtg
       61 acgtcgagga tggggcaggg tttcgggttc ctctgggcga agacgaggaa atcataggcc
      121 agatccttgg gcaggatcac caggtttccc tggacgtgcc ccttgcacat gcccggcgtg
      181 gggagagtcc actttccctc ccggatcatc tgccggactt ccctgggcga aacgccggcg
      241 taatcgaagg ccttcatccc ttcagcacct cccgtatttt tctcacttcc accccgttcg
      301 cctcaagggt tttccggata tgacgggcca tctccaccgc cgcggggtta tctccgtgga
      361 ggcagatgga atgggccgtg aggtggatgt cctttccttc cgcagagcgg atgacccctt
      421 ccttcacgag gcgcaccatg cgctcccccg cttccttcag gtcgtggacg aaggcaccgg
      481 gtttgcttct cgggacgagg gagccgtcgt ccatgtaccc ccggtccgcg aaggcctcgg
      541 cggcatacag gactccctct tcggaggcgg cctgctcgaa aagggagttg gccaggccga
      601 ggagaatgag gtctcccccg gcatccttcg tcgcccgggc aatggccttc gccagggcaa
      661 gatccttggc cgcctggttg tacagggccc cgtgagcctt cacgtgctcc aggggcactc
      721 cctgggccgc gcagaccgcc ctgagggcgc ctatctggta gagggtatag gcataggctt
      781 cgtcgggcgt gacggccatg gtgcgccgcc cgaatccctg gagatcggga tgcccggggt
      841 gggctcccac ggccacgccg gcggccttgg ccgccttcac cgttttgatc atcacttccg
      901 cgtcccccgc gtggaaaccg caggccacgt tggcggaaca gacgctttcc atgacctcgg
      961 cgtccatgcc catcttccac gggccgaaac tctcgccgag gtcgctgttc aggtcaattt
     1021 ttgacatcat tccacctccc ggatctcttc ccacgaaatt ctgtgctctt cgccgtccac
     1081 ccggatcctg gcctctccgc gggagggaag gtccggcccg gacacaggca acgggacagg
     1141 ttcccccgga gcggaccgcc acgcagacct aaggcggcgg agttcgtccc tttttcccgc
     1201 ctcttcccgg gcgagggcca gggcatcgtc caggggtatt ttctgaaagc ggaccttctg
     1261 ccccggaagc cgctgggaca gggcggcgat gtccaccgag cagaccacgc cgatcttggt
     1321 gtatcctccc gtggtctgcc tgtcggccag cataacgatg ggctgaccgt ggcccggcac
     1381 ctgcaccgcg ccgagaggaa tggcgtcgga gatgatgtcc gccgcgccgg tatgctcgat
     1441 ttcgggcccg tccatgcggt atcccatcct gtcggcgctg ttggagacgg tgtactccga
     1501 cccgtaaaaa gtgtcttttc ctttttccgt aaagaggtcg tcctggggcc cggggacaac
     1561 ccggagaggg gcctcgggcg gatagacggg ccgaagtccc tccggacata caagcccttc
     1621 gcactttctc cacagggaca ggggtgcgcc gcagcggagg gtgtctccct ttttcagggc
     1681 acggccgaga aagcccccga ccctggccct ggtataggtg gaccggctct ccatcactgg
     1741 aggcacgtcc actcctcctg aaaaacaaag ataggcgcgg catccggaca gagggccgga
     1801 gaaggacaga acatcccccg cgccgacggc cacggcagtc cacatgggag ccggccggcc
     1861 gttgatctgg aagccgaggt ccgctcccgt cgcagcgacc atcccctccc cttcagccac
     1921 aaagagggtt ggcccgagga gggtcacctc gatggcgccg tcgttctcgt cattgcccgc
     1981 aaggatgttt cctctcctga aggacggaag gtccatggct cccgccacgg gcattccctt
     2041 tccctggtat ccccacctgc cgccgtcctg gacggtggtg agcatgccgg gaagcctgca
     2101 ttccagtaca agggcactca tgacggcgcc tcctcttccc cgaggatgac gggccggtag
     2161 ctccccttga gtgacgccgc ctcgatttcg tcgaattccg ccttttcgat gggacggaac
     2221 cgtatccaca tgcccgcctc gaggaaaatg ggcggatccc tgtcggggtc gaacaggcgc
     2281 atgggcgtcc tgccgatgag tctccagcct ccgggactcg caatggaata gatgccggtc
     2341 tgctttccgc cgatggccac cgctccagcg gggatggtct cccgggggtt cttcagccgg
     2401 ggcgtttcaa gggccggatc cataccgccg aggtagggaa agccgggggt gaaccccagc
     2461 atgaaacagt agcagtccag gcccgaatgg cgccggacga cctcctccgg tgaaaagcct
     2521 gtgtgttcgg ccacgtcgtc gagatcgggg ccccattctc ccccatagag ggtgggcacc
     2581 acgatggtcc ggcgcttttc atggcctgcg gctgattctt ctttcccgca cagctcttcc
     2641 agtcgtgaat agagccgttt cacatccgtc gctaccgggt cgaagtacac cgccagggag
     2701 cggtaggtgg gaacggcgtc aagcactccg ggaaaaccga gcctcaagat ctcgttcccg
     2761 agggctgccg cccgggagtt tatctccatg tcgatggagt cgccgaattc cgccactacg
     2821 catccgtcgc ccgcggtcag aatccgggga tatttttttt cttccaacct tcaatcacct
     2881 ccatgaacgg ccggaatcgc ccacctccta ctggaacagc ttcagaattc ccgcgaagga
     2941 gttgaagccc atccacgcgg tgaagaccac gatgacccag cccagcagcg tcatccacat
     3001 ggggtggacg taatccttca cgacttcctt tttgtaggcg gcaaggagga cgcagcccag
     3061 ggacagcggg aggatcagtc cgttgaggga tccggccacg atgagcagct tcacggggcg
     3121 ccctatgagg gtgaggattg acgtggaggc gatgatgaaa ccgatgatcc agaaccggta
     3181 gtatttgtca acgacaccga acagcgtccg gaggaaggag acggaggtat aggaggcccc
     3241 gaccacggag gtgatggccg ccgcccagag aaccactccg gcgattctgt acccgagggg
     3301 gccggcgccg atctggaagg ccgaggccac ggggtttttc atgtcgattt cggtgccgag
     3361 aagaacaaca ccgaggaagg ccaggaacag aaggtagcgc atgacgccgg tgatggcgat
     3421 gccgttgatg gaccccttgc tgatctggct gaggtgttcc tttcctgtga tcccagcgtc
     3481 gatgatcctg tgggcgccgg agaaggtgat gtagccgccc acggttccgc ccacgagggt
     3541 gagcaccacg agccacttgt actctttcag gaggttcatg tcggtaaagg gcttcaccat
     3601 ttcatgcacc gtctccatga cgggaggctt tgtgacgaac accacgtaga acaccagggc
     3661 gagcatgagg aagccgagaa tctgggtgaa cttgtccatg gccttgccga ggtctttcca
     3721 gaggaagatt gtgatggcga tggccgcgct gatggacgct ccgacaacct gctcggagcc
     3781 gaaaagcacc tcgaagccga gggcagctcc acccacgttg cccacgttga aggccagtcc
     3841 gccgagggcg accgcgaagg cgaggaagta gccaagtccc ggaagaacct tgttggccac
     3901 gtcctgggcc ctcatgcccg agacggcgag aatgcgccat atgttcagct gcactacgat
     3961 atcgatgaga atggagagga gaatcgcgaa ggcaaaggtt gctttcagtt cattggtgaa
     4021 tgaaatcgtc tgggtaagga accccggccc gatggccgag gtggccatga ggaacgccgc
     4081 tcccaaaagc acgctgagag tctcacttct gcttctttcc tgcgtcttgc ccaaatctgt
     4141 catagcgaca cctccatgag atatgtgata aaaaagcttg atgcagcaag cttattatac
     4201 gtgattttct cgtaagggaa agaagagtag tttttcacga ttcggactga ccaaaagatc
     4261 acttttccgg aagaaagagg cagatggaat aaaaccgtta caccgccgtt gcatttcttt
     4321 gctacaataa ccctggaaac aggttcggga agggagtcct gaccatgatg cgccgcgccc
     4381 tgtggctttc gggaggaatc tgctggttcg cctacgttct ggcgcctctt gccctcattc
     4441 ttgtcggatc gttcggcgag aagtggttcg gtaccctgct gcccgacggg ttcaccctga
     4501 ggtggtacgc cgatctcttc tcaaagacca tgtacgtccg ggccatgggg atgagcctcc
     4561 tcgtcgcctg ccttaccgtt ttcataaatg ccctcgtggg gatatgttcc gtctacgccg
     4621 tgtccgtcct cgggaagaaa tggctccggc gggccttcga gttcgtcatc ctgcttccca
     4681 tcgccattcc tcccgtggtc atggggctcg gcctcgtcca gggattcaac tggccttcct
     4741 tttcgctggt aggcacctgg cagcttctcc tgggcgccca tctcgtgtat acccttccct
     4801 tcatgctccg ccctctcatg gccaacatgg acatgctgaa ctggaccgtg ctcgaggaag
     4861 cgggaacgtc cctcggcgca acgcctttct tcctcgcgac ccgggtcctt gtgcccaacc
     4921 tcgtccccgg gctgctctcc ggggccatta tgacctttgc catgtcgctg ggagagttcc
     4981 agctcgcggt catggtcacc ggctcggcaa gccagacgta cccggtggtc ctctaccagg
     5041 ccttttacgt gagcacggga ttcgcctgcg cggccactac cctgctggtc ctcttcagca
     5101 tcctgagcct cggcgccgtg atcttcctcg ggaggctgtt cggcaccagg agcgcgggaa
     5161 tggcggtata aaatgacaat cacggaaagg agcacatccg gaatggatcg aagccccatc
     5221 atttcagtca tcggaacgtc caccgcgtct cccgggcttt acgaactggc ccgggaagtg
     5281 gggcggctcc tcgcccgggc aggatgcacc gtggtatgcg gcggaagggg cggcgtcatg
     5341 gaagccgtct gccggggggt ctccgaggaa ggggggacat ccgtcggcct tcttccggga
     5401 gacatcaggg aagcgaatcc ctatgtcacc attcccctct ccaccggact cggggaagtg
     5461 cggaatttcg ccgtggcctc cgccggagag gcggtcatct ccatcggcgg cgctttcggc
     5521 accctgtcgg agatagggtt cgccctcaga tccggaaaac cggttattgc gttgaagtcg
     5581 tggcacatat ccgaaggcgg ggaatcaccg tcgccgatga tcgaggcggc gacggccgaa
     5641 gaagctgtcc ggctggccct tgaaaaaaca gggagggaat cccatgacag gggttgaact
     5701 gagatctgtc agcaagcgat tcgggaagac ctacgccgtc cggaacgttt ccctctccgt
     5761 ggggaaggga gagtttctct ctcttgtcgg cccctcaggc tgcggaaaaa ccaccaccct
     5821 gaggctcgtg gcaggatttc tcgcccccga cgagggtgaa gtgctcctga acggagaatc
     5881 catgggcgga gtgggcgtcc ggcagcgaca ggtgggcatc gtcttccaga actacgctct
     5941 tttccccaac ctcaccgtgt tcgaaaacgt ggccttcgga ctccggacca ggaacacgcc
     6001 ggaggatgtt ctgcggccga aggtggaaca gcttctttcc atggtgggac tcgaccggcg
     6061 ggcctcggcc tggccacggg agctttcagg cggccagcag cagcgggttg cccttgcgcg
     6121 ggcccttgcc atcacgccga aggtcctcct cctggacgaa cctctctccg ccctcgacgc
     6181 caaggtccgg aactccctcc ggttcgagat aaagagaatc cagagggaga gcggcatcac
     6241 cacgatctac gtgacccacg accaggaaga agccctctcc atctcggacc gggtggccct
     6301 gatgaacgag ggcaggatcg agcagacagg ctcccccagg gatatctacc ttcgccccgc
     6361 gaattccttc gtcgccgatt tcgtcggggt caacaacctg ctggagggag aatatctcgg
     6421 cgacggcagg ttcaggtgga gggaaagaat cctctcggtg gagaatgttc cccttcccca
     6481 gggcccctgt tcactcatga tacggcctga gcggatttcc atcgcatccc gggattccgc
     6541 ctcaggggac ggcaatatcc ttccggggag agtgtcggga aaggtgttcc tcggtcccct
     6601 tctccgcctt gcggt
//
LOCUS       DAOTHD010000010         6567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_13898_length_6567_cov_3.174447, whole genome shotgun sequence.
ACCESSION   DAOTHD010000010 DAOTHD010000000
VERSION     DAOTHD010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 6567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6567
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13898_length_6567_cov_3.174447"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="P5219_00385"
     CDS             <1..388
                     /locus_tag="P5219_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRX25241.1"
                     /translation="ECLAIRLAAERIGDRHLRRLEEALVEEARAIEERDLETYLEVNE
                     AFHKAVADASGNRVLAEYVENILARTNAYVVFYDPFYQNETTPTVDSHKEILMALRAH
                     DSEKSVKLMKRHLKESISGLSRIVEE"
     gene            538..1671
                     /locus_tag="P5219_00390"
     CDS             538..1671
                     /locus_tag="P5219_00390"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRX25242.1"
                     /translation="MTAKIPTLDLTRNYGRIKEEILEALNSVLESQHFIMGPDVAAFE
                     KECESYLGGVSAIGCASGTDALLLALMALDIGEGDEVITTPYSFFATVSCITRLGAVP
                     VFADVDPGTFNIDPAKVLEAVTPRTKAFLPVHIFGQMTPMEIVYEELSARGVAVVEDA
                     AQAFGAWRKEGNLIRRAGAWGKIGCYSFFPTKNLGGFGDGGMIVTRDEEIASRLRKLR
                     VHGAGTTYYHDEVGLNSRLDSLQAAILRVKLRHLEEWNEERRTVAGRYFALFAEKGLL
                     DTVVPPQELEGNFHIYHQYVVKVPRRDELQAFLEDEGIVSRVYYPVSLHLQKCFSFLG
                     YSEGDFPVSEQLSRETLALPVFPELTMEEQERIVSAIRRFYDK"
     gene            1783..2478
                     /locus_tag="P5219_00395"
     CDS             1783..2478
                     /locus_tag="P5219_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HRX25243.1"
                     /translation="MFPFFFDPTMMLLIPALLLAMWAQMRVKSVFAQYSAVGSRRGVT
                     AASAARAILDRFGLQAVPVNRVGGNLTDHYDPRDKSLSLSDSVYGSTSIAAIGVAAHE
                     VGHAIQDSVNYSPLKIRNTIVPVVGVGSTLAFPLFFIGILLRGQMLMDLGILLFLGVI
                     LFHLVTLPVEFDASNRALRVLADTGILSTDEIGGAGSVLRAASWTYIAATVMAFAQLI
                     RLLFLRGMFGSRD"
     gene            2572..3249
                     /locus_tag="P5219_00400"
     CDS             2572..3249
                     /locus_tag="P5219_00400"
                     /inference="COORDINATES: protein motif:HMM:NF039426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HRX25244.1"
                     /translation="MPFFVFVLLLFLFPWLAFAGLLLVIVLVSVGFTAKYVPGIFFSP
                     AKLLRILFNKRVRANHGLAHGTISVLQERYGPLEIEGPAEEDGFSLRGGFDPEEVLSA
                     ARNALLRIRSGESSLVLSPKCAAASAVVLFCAIPCALLLALLEGFSALNAAIVLLLSF
                     LLVRFTSPWIQRRLTFPLNLQGIEITGAESRKERKTFFGVELLVPSSVFVRTRFKGEP
                     LVAEVVS"
     gene            3246..4583
                     /locus_tag="P5219_00405"
     CDS             3246..4583
                     /locus_tag="P5219_00405"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HRX25245.1"
                     /translation="MRGIEAALLVWREVRAGKFASESIRRIADSLSPGDLTLVSSLVY
                     SALRRQFLWKEIYSRFLRTSPSGLSQSAGDALCIGTAGILELRTFAPRVLVNGLVQEM
                     KKQGDDRGSRIVNAVLRRVVEEGRDQLKKIEGSGGLKEQSLVSGVPSWVASSWRNIWG
                     DDGKKLVKMMRIRPYSSFRITGEADRERILGLAKEKGIRCWQSPFVSSSVRLAGTVFP
                     PDFPGYGEGLATPQTESSMMVAEVMKKMHRGGPLLEMCSGRGVKTGHISSLFRETPVE
                     SIELSPARAKAAERELARTGRRGKVRIITGDALQTLPSFIPAMISLDAPCSGSGTWNR
                     HPEAKWRLTPEKLDSLASLQINLLKRAVSLLAPGGIVVYSTCSLLKNENENVVAHVLS
                     EEPGLVELSFPFSGPVFRKGRPWGTYMWPELPWIDGFYMAVIMKKSGGKTVDG"
     gene            4576..5736
                     /locus_tag="P5219_00410"
     CDS             4576..5736
                     /locus_tag="P5219_00410"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRX25246.1"
                     /translation="MGRIFRWAVVLVILVILISGSVAFYTVFFGGKDLVIPPLREMSV
                     LDAVDEAERIGLEVKIEQVDSSIPAGTVLAQWPEAGTKVRRDKSIILKISRGGNKKAV
                     PDLRGLENTQARKKIQELGFTEGDTVKIHDDSRPAGTVIAQNPAIPAVVDDARKIDLL
                     VSLCPVPKDGRIPVPDIAQRDEAAGKDLLAQSGLKFGGAEYVSTQNTPEGMIMSTKPK
                     AGTMVRLGDSVRIVVATNKRKPEPQKPESQVVAPGSAGRPSETVVVEDPPAQQTPRQV
                     GPAGPVQTVLTPQQQELAGATGLPSASQPAASGQKTGPAPTGTAKIRYQVPPLTKPLG
                     LKIEMVDATGTRHLLGRDVKGGEFISLDAPFVREGVVTIYLGGEFVWQERYK"
     gene            5736..6413
                     /gene="rpe"
                     /locus_tag="P5219_00415"
     CDS             5736..6413
                     /gene="rpe"
                     /locus_tag="P5219_00415"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRX25247.1"
                     /translation="MRNVFLAPSLLSADFLDVRSSIASLKGQHDWLHVDVMDGCFVPN
                     ITFGPGFVSALRKGYPSEVLDVHLMIEHPERLLEAFLDAGPDYLTVHIEADPHIHRTL
                     SRIRERGAKAGVSINPGTPEELLRPLLPMVDLVLVMSVNPGFGGQSFIPSVMEKTKSL
                     CRWREAGHYSYVVEMDGGIGGGNAAEIALGGCDVLVMGSAVFGAQDPALFLREIKLKV
                     KEAIAHA"
BASE COUNT         1461 a         1719 c         1872 g         1515 t
ORIGIN      
        1 tgaatgcctt gccatccgac ttgcagcgga acgaatcggc gacaggcatc tccggaggct
       61 ggaagaggct cttgtggaag aagcgagggc gatcgaggag agggatcttg aaacctatct
      121 cgaggtgaac gaagcattcc acaaagctgt tgccgatgcg agcggcaaca gggtgcttgc
      181 ggaatacgtg gagaatattc tcgcacggac aaatgcctac gttgtattct atgatccttt
      241 ctatcagaac gaaaccaccc ctacagtcga ctctcacaag gaaattctga tggctctccg
      301 ggctcacgac agcgagaaat cagtcaagct catgaagcgc catcttaagg aatccatttc
      361 gggactcagc aggattgtgg aggaatgacc tgcccatagg gtcatggctt ccgggcggga
      421 atcttttccc gctcgttttt tgtgggccac aaaatggccc gatttggtga agatacacta
      481 cggagtgcat tcgacgcgaa gtttttttcg cgaacgttca aggaggtaca cgcttttatg
      541 actgcgaaaa ttcccactct ggatcttacg agaaattacg gaaggataaa ggaggaaata
      601 ctggaagccc tgaactctgt tcttgaaagc cagcacttta taatggggcc ggatgtggcc
      661 gcttttgaaa aggaatgtga aagctacctc ggcggcgtct ccgccatcgg gtgtgcttcg
      721 ggaaccgacg cacttctcct ggcgctcatg gctcttgaca taggagaagg agacgaggtt
      781 atcacaacgc cctacagttt cttcgccaca gtaagctgca ttacccgact cggggcggtt
      841 cccgttttcg ccgacgtcga ccccggaacc tttaacattg acccggccaa ggttcttgaa
      901 gctgttacgc caagaaccaa agcattcctt ccggttcaca tcttcggcca gatgacgccc
      961 atggagatcg tctatgaaga actatccgcc aggggagtgg ctgtcgttga ggacgcagcc
     1021 caggcattcg gggcctggag aaaggaagga aatcttatcc gccgggcggg agcctggggg
     1081 aaaataggct gctactcctt tttcccgacg aagaacctgg gaggattcgg agacggcggg
     1141 atgatagtca cccgggatga agaaattgcc tcccgcctca ggaaactcag ggtgcacggc
     1201 gcaggcacga cctactacca cgacgaggtc ggcctgaaca gcaggcttga ttctcttcag
     1261 gccgccatcc tcagggtcaa gctccgtcat ctcgaggagt ggaatgaaga gcgccgcacg
     1321 gtagccggga gatattttgc cctttttgca gaaaagggac tgctcgatac ggtagttccc
     1381 ccccaggaac tcgaggggaa ttttcacatt tatcaccagt atgtggtcaa ggtgccccgc
     1441 agggacgaac ttcaggcttt ccttgaagat gaaggaattg tgagcagagt gtattacccc
     1501 gtcagcctcc atcttcagaa atgcttctct ttcctgggct actcggaagg ggatttcccc
     1561 gtatctgagc agctcagcag ggaaaccctc gcccttccgg ttttcccgga acttaccatg
     1621 gaagagcagg aacgtatcgt ctcggctatc cgccggttct acgacaagta gactgaccaa
     1681 tattgatcta cacgccggaa ccgtgtaaaa taccgatgaa gatagaacgg aacaggagta
     1741 atctttcgtt catctaccat gtattttgac ggaggtgggg ccatgttccc ctttttcttt
     1801 gatccgacga tgatgttgct gattccagct cttcttcttg ccatgtgggc acagatgagg
     1861 gttaaatcag tgtttgcgca gtacagcgcg gtggggtccc gccggggggt tacggcggcg
     1921 tccgccgccc gggcgatcct ggaccgcttt ggtcttcagg ctgttccggt gaaccgggtg
     1981 ggaggaaacc tcacagatca ctatgacccc agggacaaga gcctcagcct ttccgattcg
     2041 gtgtacggca gcacgagcat tgcggccata ggtgttgccg cccatgaagt gggacatgct
     2101 atccaggaca gcgtgaatta ctcgccgctg aagatacgga ataccattgt tcccgtggtg
     2161 ggagtcggtt ctacgctggc ttttccactt ttcttcatcg gcatcctgct tcgcggccag
     2221 atgctcatgg atctcggcat actccttttt ctcggagtca tcctattcca tcttgtaacg
     2281 ctgccggtgg aattcgacgc cagcaaccgt gcactgaggg ttctcgccga tacgggaatt
     2341 ctctccaccg atgaaatcgg cggtgcggga tccgtgctca gggctgcatc ctggacctat
     2401 attgccgcga cagtcatggc tttcgcccag ctcatacgcc ttcttttcct ccggggaatg
     2461 ttcggcagca gggactgaat cctgccgggg cggctcggac atcattaccg ggaaaatgga
     2521 gcggctccgg aagtcgctcc atttcttttc ctggaaagaa aggaacaatg catgcctttt
     2581 tttgtattcg tccttctcct ttttcttttt ccatggctgg cctttgcggg ccttcttctg
     2641 gtgatcgttc tcgtgtcggt gggttttaca gcgaagtatg tgcctggaat ctttttttcg
     2701 cccgcgaaac ttcttcgcat tcttttcaac aaaagggtgc gggcaaatca cggacttgcc
     2761 cacggaacga tctcggttct ccaagagcgg tacggtcccc ttgaaatcga aggaccggcc
     2821 gaggaagacg gtttctccct caggggaggg ttcgaccctg aggaagtgct ttccgccgcg
     2881 cgaaacgccc ttcttcgaat ccggtcaggg gaatcatccc ttgtcctgag tccgaaatgt
     2941 gcggctgctt cggctgtcgt tttattctgt gcgattccct gcgctctcct tcttgctctt
     3001 cttgaaggct tcagtgctct gaacgccgca atcgttctgc ttttgtcctt ccttttggtc
     3061 cgtttcacct ctccctggat tcaacgccgc cttacctttc ctctgaacct gcagggcatt
     3121 gaaataacgg gtgctgaatc ccgaaaggaa agaaagactt ttttcggcgt cgaattgctc
     3181 gtaccgtctt ccgttttcgt cagaacaaga ttcaaaggag agccccttgt cgctgaggtg
     3241 gtgtcatgag gggaattgaa gcggccctcc ttgtgtggcg ggaagtgcgg gccgggaagt
     3301 tcgcttccga atcgatacgg aggattgccg attccctctc gccgggagac ctcaccctgg
     3361 tttcatccct tgtctattcc gccctcagaa ggcaatttct ctggaaggaa atctacagca
     3421 ggtttctcag gacatcaccg tcaggactct cccagtctgc cggtgatgcg ctgtgtatcg
     3481 gtacggccgg tattctcgaa cttcgaacct ttgctccaag ggttttggta aacggtcttg
     3541 tccaggaaat gaaaaagcag ggagacgacc gcggttcccg cattgtgaac gcggttctgc
     3601 ggcgagtcgt cgaggagggc cgggaccagc tgaaaaagat agagggttcc ggtgggctca
     3661 aagaacagtc actggtttca ggtgttccgt catgggtggc ttcctcgtgg cggaatatct
     3721 ggggagacga tgggaaaaaa ctcgtcaaaa tgatgcggat acgcccctat tcatccttcc
     3781 gcataacagg agaggctgac agggagagga tactcgggct tgcgaaggag aagggaatac
     3841 ggtgctggca gtctcccttc gtttcctcct ccgtgcgcct ggccggaacc gtttttcctc
     3901 cggattttcc cgggtacgga gaaggccttg caacgcccca gactgaatcc tccatgatgg
     3961 ttgctgaagt gatgaaaaag atgcacagag gggggcctct tcttgaaatg tgttccggga
     4021 gaggagtgaa aacggggcat atatcctctc tttttcggga aactcccgtg gaaagcatcg
     4081 agctgtcccc tgcacgggcg aaggctgcgg agagagagct tgccaggacg ggaaggaggg
     4141 ggaaagtcag aattatcacc ggggatgcgc ttcaaaccct cccatctttt attcccgcaa
     4201 tgatttctct tgatgccccc tgttctggaa gcggaacctg gaaccgccat ccggaggcaa
     4261 aatggcgact gaccccggaa aagcttgatt ccctggcatc cctgcagata aatctgctga
     4321 aaagagcggt atccctcctt gcgccgggag gaattgtggt ctactctact tgtagcctgc
     4381 tgaaaaacga aaatgaaaat gtggtcgccc atgtcctctc ggaagagccg gggctggtgg
     4441 aactgtcctt tcccttcagc ggacccgtgt tcaggaaagg acgtccctgg gggacataca
     4501 tgtggccgga actgccatgg atcgacggat tttacatggc cgtgatcatg aaaaaatcgg
     4561 gagggaagac tgttgatggg tagaatcttc cgctgggccg tggtcctggt gattcttgtc
     4621 attctcatat cgggcagcgt ggctttttac acggtgtttt tcggagggaa agacctcgtg
     4681 atacctcctc ttcgggaaat gtcggtactg gacgccgttg acgaagcaga gcggatcggt
     4741 ctagaagtga aaatcgaaca ggtggattcc tccatacctg cgggaaccgt tctcgcccag
     4801 tggccggaag cgggaacaaa ggtccggagg gacaagagca taattctcaa aataagcagg
     4861 ggcggcaaca aaaaagcagt ccctgacctc aggggacttg aaaacaccca ggcccggaaa
     4921 aaaatccagg agcttggctt taccgaggga gacacagtta agattcacga tgactccagg
     4981 cctgcaggta ccgtgatagc ccagaatccg gcaatacctg ccgtagttga cgatgccaga
     5041 aaaattgacc tgcttgtcag tctttgtcct gttcccaaag atgggcgcat ccctgtcccg
     5101 gacattgccc aacgggatga agcggcaggg aaggatcttc tcgcccagag cggcctgaaa
     5161 ttcggagggg cggaatacgt ttcaacccag aacaccccgg aaggcatgat aatgtcaaca
     5221 aagcccaaag cgggaaccat ggtccggctg ggagattcag tccggatcgt cgttgccacg
     5281 aacaaaagga aaccggaacc ccagaagcct gagagccagg ttgtggcacc cggctccgca
     5341 gggcgtccat cggagacggt cgtagttgag gatccgcctg cacagcagac gccgcggcag
     5401 gtggggcctg ccggacccgt tcagactgtg ctgactccgc aacagcagga gctcgccggc
     5461 gccaccgggc tgccgtcagc ttcccagcct gcggcgtccg gtcagaaaac cggaccggcc
     5521 ccgaccggca cggcaaagat ccggtaccag gttccgcccc ttacaaaacc gcttgggctg
     5581 aagatcgaga tggtcgacgc caccggaacc aggcatcttc tcggccggga tgtcaaagga
     5641 ggagaattca tatcccttga cgctcctttt gtacgggaag gggttgtcac catttatctc
     5701 ggaggagagt ttgtctggca ggagcgctac aaatgatgag gaatgttttt ctcgctccct
     5761 ccctcctttc tgcggacttt ctcgatgtcc ggtcttcgat agcttccctg aagggacagc
     5821 atgactggct tcatgtcgat gtcatggacg ggtgttttgt tccgaacatc accttcggtc
     5881 cgggctttgt ctcagccctg aggaaaggat atccgtcgga ggtgcttgac gtccatctca
     5941 tgattgaaca tcccgaaagg ctcctggagg cattccttga tgcgggcccc gactatctta
     6001 cggttcatat agaagcggat cctcatatcc acaggaccct ttccaggatt cgcgaacgag
     6061 gcgcaaaggc cggagtatcc attaatccgg gaaccccgga agaactgctt cgcccccttc
     6121 ttcccatggt ggaccttgtt ctggtcatgt ccgtaaaccc cggtttcgga gggcagtcat
     6181 tcattccctc cgtaatggaa aaaacaaaat cattatgcag gtggagggaa gcggggcatt
     6241 attcctatgt tgtggaaatg gatggtggaa taggcggggg gaatgccgcc gaaatagctc
     6301 tcggcgggtg tgacgttttg gtaatgggaa gcgccgtgtt cggcgctcag gatcctgccc
     6361 tgtttttgcg ggagatcaag ctgaaagtta aggaggcaat cgcccatgcc tgagcatcgg
     6421 gacaatactt tgcaggactt ggggaaggaa atcagacgca agagagaaga actcggactc
     6481 agcctgaagg tcgtccacga gggaacaaag ataagagtcc agtttcttga aggaatagaa
     6541 cggggtgatt tttccgaatt tcccgga
//
LOCUS       DAOTHD010000011         6504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_14091_length_6504_cov_3.205148, whole genome shotgun sequence.
ACCESSION   DAOTHD010000011 DAOTHD010000000
VERSION     DAOTHD010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 6504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6504
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14091_length_6504_cov_3.205148"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1475
                     /locus_tag="P5219_00420"
     CDS             <1..1475
                     /locus_tag="P5219_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRX25248.1"
                     /translation="CLTRRKTVIASVFVLLVLSMAAFSMVDKSFFPNSTSPMFTVDFW
                     RIRGTGVEATLDDGRRMQAFLSVQPETKSVAVYAGEGALRFILTYTPGDPASNYGHLI
                     VEATDSEGAERLKSRLAEFVRAELPHLDPRIRSFSKGTGGGAKVQVRFLGEDPDTLRD
                     LRNKAVAVLKEDPDSINIRDNWGERVKVIRPVLGDTIQELGLTRKDVANALKASYSGL
                     RVGLFREGEKLVPMMARLPAEERGSLENLGETQIWSALSRRYIPLAQAAQDIEVTAED
                     PAVYRINRMRALTVEADSGSGSAAALFSRVREKIESLPLPPGTTMEWGGEYENSRKAQ
                     GGLMGMIPVAFMVIVVILVALFNGLRQPLIILLCLPLSVMGLSVGLLVMGKSFDFLAL
                     LGFLSLAGMLIKNAIVLIDQIDLEIRLGKAPFDAILDSGISRCRPVMMAALTTVLGMI
                     PLYFDVLFSAMAVTIMFGLSFATVLTLVVVPVLYAEALKA"
     gene            1614..2855
                     /locus_tag="P5219_00425"
     CDS             1614..2855
                     /locus_tag="P5219_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HRX25249.1"
                     /translation="MSLNVDISRLMSRLEILSGFNATPGKGITRFSYSPEDREARKYL
                     EALFSSMGLKTTVDGAGNMRARLEESDPGAPAVLSGSHIDTVLHGGKYDGAVGTLGAI
                     EAVQAIMESGLPRRCPLEVAVFAEEEGSNFGSTLAGSKALVGKYTLAHMEKLKTPEGK
                     SMAGLLREGGYAPESMADNLLRPETIKAMVELHIEQSVVLESRGVPVGIVEAVAGIRV
                     LEIRIRGVPNHAGATPMTLRQDPLAAGARLMGTIEDLAKKSATGSTVATVGKILCRPN
                     VSNIIPEEVTFTVDIRDVQDAGIESVAAGIEKAAEALAASRDVSVDVVHISESAPVLL
                     DGHIAGVTETCARRRNFRYARMNSGAVHDACMFAPLVPTGMIFIPSKGGRSHVPEEFT
                     GQEDIGKGVSLLADVLYELSK"
     gene            2953..3645
                     /locus_tag="P5219_00430"
     CDS             2953..3645
                     /locus_tag="P5219_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HRX25250.1"
                     /translation="MDEVLKVANSMGAWLTAAPIVIIALVQVLLYYRQIYHAADAVNL
                     TREQLSQAFRTGMVTSIGPVVAIFIIMVGLMSVIGAPMAWMRLAVIGAAPTELTAATL
                     GADAMGVKFGGEGYTLQVMAVSWWTMAINGVGWLLLVGLFAHKLEDVREKVGGGDTKW
                     LGILSTAAMIGVFGYLNSRNLTAVSGPLVAAVTGAVSMVLLLQVAKKMPQIKEYTLGI
                     AMLIGMFAAMFY"
     gene            3665..4342
                     /locus_tag="P5219_00435"
     CDS             3665..4342
                     /locus_tag="P5219_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25251.1"
                     /translation="MAAEQINEQKYHEVWTTPVIRLGISTMLLACVLSFIPLIYLYMV
                     HGILPPFSAALTAWGLVASVFGAFYIVEPISYYPILGLAGTYISFLSGNISNLRLPCS
                     AMAQEVVGVEEGTKQAEIIGTMGIIGSVVVNLAGVTLAAFVGAWIIGLFPAFVADAFR
                     KFTAPAIFGAVFGQFAMRQPKLAPFALAIPLTLLHFKAPIWLVILASVFGTIAISRIF
                     YKAKLIH"
     gene            4505..5755
                     /locus_tag="P5219_00440"
     CDS             4505..5755
                     /locus_tag="P5219_00440"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRX25252.1"
                     /translation="MNKRNFAPALLVILTVLTLSGSLFAEEKTISLEECLALAEANHP
                     ALEEARAALAGQQAKLGQVRVSNALKGNLSASGSRNSGSDGSYTTSFSVSKLLSDSGK
                     NALERKSQGLSVDAASESQRETVLSVRTGVKDAYYGLLLAMRKRDQAESAVKTYQRHL
                     DKARGFYDAGVKARFDVTKAEVDLSNARIDLVSAESSLETARAALSRAVGVPLDHARP
                     VSDFLAPRDIPAENFALEQALENRPDIRSSRLKSRAGKLGISIAAKGNAATISVTGSA
                     KLSGTGLPPDDDYSVGITLSVPVFDGGLTDYRIAEARASADGLDASLKKLEQTVRYEV
                     RSALLSVREAEARIPAAELLVRQAEENLTLAEGRYEMGVGNVLEVADALLAFNSARVS
                     HFQALHDYSTAVSDLEQTLGGEFK"
     gene            5752..>6504
                     /locus_tag="P5219_00445"
     CDS             5752..>6504
                     /locus_tag="P5219_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRX25253.1"
                     /translation="MKKVFILLILIAAGFAYLRFSHSDGPRYTFRTVPLEKGDITSTV
                     TATGKLEAVTVVEVGTQVSGTLKEIYADFNQHVKKGELIALIDPDVLKAKLEEARANL
                     AVARASAARARANLAESDRNLKRYKELWNRQLIARSELDAVETTRLTNRASVQEADAR
                     VLQVQASLRQAETNLEYTKILSPEDGVIISREVNVGQTVAASLSAPVLFTIAKDLSDM
                     QIETSVDEADISRVKEGQEVEFSVDAYSGTTFK"
BASE COUNT         1297 a         2061 c         1930 g         1216 t
ORIGIN      
        1 tctgcctcac ccggcgaaag accgtcatcg cctcggtgtt cgtcttgctg gtcctctcca
       61 tggctgcctt cagcatggtg gacaaatcct tcttccccaa ctccacgagc cccatgttca
      121 ccgtggattt ctggcggatc aggggaaccg gcgtggaagc cacccttgac gacggccgga
      181 gaatgcaggc cttcctttcc gttcagcccg aaacgaaatc agtggccgtc tacgcgggag
      241 aaggagcgct gcgtttcatc ctcacctaca cccccgggga ccctgcgtca aactacggcc
      301 atcttattgt ggaggctacg gattccgagg gagctgaacg gctgaaaagc aggctcgcag
      361 aatttgtccg ggccgaactg ccccatctcg acccgagaat ccgctctttc agcaagggaa
      421 ccggcggcgg cgcgaaggtc caggtccgct ttctgggcga ggatcccgac accctcaggg
      481 acctccggaa caaagccgtg gccgttctga aagaagaccc cgacagcatc aacatacggg
      541 acaactgggg cgaacgggtc aaggtcatcc gccccgtcct gggcgatacc atccaggaac
      601 tcggcctgac cagaaaggac gtggcgaacg ccctcaaggc gtcctattcg ggactgagag
      661 tcgggctctt ccgggaaggg gaaaaacttg tccccatgat ggcccgtctt ccggcggaag
      721 agcggggcag cctggagaat ctgggcgaga cccagatctg gagcgccctc tcccggcggt
      781 acatccccct cgcgcaggcg gcacaggaca tcgaagtgac cgccgaggac ccggccgttt
      841 accgcatcaa ccgcatgagg gctctcaccg tggaggccga cagcggttca gggtcggctg
      901 ccgccctttt cagccgagtc cgggaaaaga ttgagtccct tcccctgccc cccggcacca
      961 ccatggaatg gggcggtgaa tatgaaaact cacgcaaagc ccagggcggt ctgatgggaa
     1021 tgatccccgt ggccttcatg gttattgtgg ttattctggt ggccctcttc aacggcctcc
     1081 ggcagcccct catcatcctt ctctgcctgc ccctgtccgt catgggcctt tccgtggggc
     1141 tgctggtcat gggaaaatcc tttgacttcc tcgctctcct ggggttcctg agcctggccg
     1201 ggatgctcat caagaatgcc attgtcctca tcgaccagat cgacctcgaa atccggcttg
     1261 ggaaagcccc cttcgacgcc atcctggatt cgggcatcag ccggtgccgt cccgtaatga
     1321 tggcggctct caccaccgtc ctcggcatga tcccactcta cttcgacgtt cttttctccg
     1381 ccatggcggt gaccatcatg ttcggccttt ccttcgccac cgtgctgacc ctggtggtcg
     1441 ttccggtgct ctatgccgaa gcgctgaaag cctagttccg ccccgttcga cgcatccggc
     1501 cccatgatgg ggccggatgt tttttcaaca cttccaattt gccctttatt tcgtctcttt
     1561 tcggttctat aatatgaaat gtttttgaca tgttcagaga ggagtgaagt gtcatgtcgt
     1621 tgaatgtgga catttcccgc ctgatgagcc gcctggagat cctgagcgga ttcaacgcca
     1681 ctccgggaaa gggaattacg aggttttcct attcacctga ggacagggaa gccaggaaat
     1741 atttggaagc acttttctcc tccatgggcc tgaaaacgac tgtggacgga gcgggaaata
     1801 tgcgggcgcg cctggaggaa tcagacccgg gcgcgcccgc cgttctctcc ggttcccaca
     1861 tcgacaccgt ccttcacggt ggaaagtacg acggcgcagt aggtaccctt ggggccatcg
     1921 aggctgtcca ggcaatcatg gaaagcggcc tcccccgccg gtgtcctctc gaagtggcgg
     1981 tgttcgccga ggaggaaggg tccaatttcg gctccaccct cgcgggaagc aaggctctcg
     2041 tgggaaaata caccctcgcc catatggaga aactcaaaac cccggaagga aaatccatgg
     2101 ccggcctgct ccgggaaggg ggatacgccc cggaatccat ggcggacaac ctccttcgtc
     2161 cggaaacaat caaggccatg gtggaactcc acatcgagca gagcgtggtg ctcgagtcac
     2221 ggggagttcc agtgggcatc gtcgaagctg tggcgggcat cagggtcctt gaaatacgga
     2281 tcaggggcgt gccgaatcat gcgggggcca cccccatgac gctccggcag gatcctctgg
     2341 ccgccggggc ccggctcatg ggcaccatcg aagacctggc gaagaaatcg gcgacgggct
     2401 ccaccgtggc gacagtcgga aagattctct gccgccccaa cgtgtccaac atcattccgg
     2461 aagaagtcac gttcaccgtt gacatcaggg atgtgcaaga cgccgggata gaatccgtcg
     2521 cggccggaat agagaaggcg gccgaagctc tcgccgcctc gagggacgtc tccgtggacg
     2581 tggtgcacat ctccgaatcc gccccggttc tgcttgacgg gcacatcgcc ggcgtaacgg
     2641 aaacctgcgc ccggagaaga aattttcgct atgcgcgcat gaacagcggc gccgtccacg
     2701 atgcctgcat gttcgccccc ctggtgccca ccgggatgat cttcatcccg agcaaagggg
     2761 gcagaagcca cgtgcccgag gagtttaccg ggcaggagga cattggaaag ggggtctcgc
     2821 tgctggcgga cgtactgtac gaactttcga aatgaaagga ggcgggaagg acacatccgg
     2881 aacacggcgg ccaacatagg gagaaagagt gctgaaaagg ctgttcattg agaggaaagg
     2941 gaggtgtctt gcgtggacga agtgttgaaa gtggcaaatt ccatgggtgc atggctcacg
     3001 gccgctccaa tcgtcatcat cgccctggtc caggtgcttc tgtactaccg gcagatctac
     3061 cacgccgccg atgcggtgaa cctgaccagg gaacagcttt cccaggcctt ccgcaccggt
     3121 atggtgacgt ccatcggccc cgtggtggcc atcttcatca tcatggtggg cctcatgtcg
     3181 gtgataggcg cccccatggc ctggatgcgc ctcgccgtca tcggcgccgc tcccacggag
     3241 ctgacggcgg ccaccctcgg cgccgacgcc atgggagtga agttcggagg agagggctac
     3301 actctccagg tgatggcggt ctcctggtgg accatggcca tcaacggcgt cggctggctc
     3361 ctgctcgtgg ggcttttcgc ccacaagctg gaggatgtcc gggaaaaggt gggcggcggg
     3421 gacacgaaat ggctgggaat cctttccacg gcggccatga tcggcgtgtt cggctacctg
     3481 aacagccgga atctgacggc ggtgagcggc cccctcgtgg cagccgtgac gggggccgta
     3541 tccatggtcc tcctgctgca ggtggcgaag aaaatgccgc agatcaagga atacaccctc
     3601 ggcatagcca tgcttatcgg catgttcgcc gccatgttct actagggaag gagaggtgac
     3661 tgaaatggca gcggaacaga tcaacgagca gaagtaccac gaggtatgga ccacgccggt
     3721 catccggctc ggcatcagca ccatgctcct ggcctgcgtg ctgagcttca tccccctcat
     3781 ctacctctac atggtccacg gcatcctgcc tcccttctct gccgcactca ccgcatgggg
     3841 gcttgtggca tccgtcttcg gcgccttcta catcgtggag cccatctcct actaccccat
     3901 cctcggcctt gcggggacct acatctcctt cctcagcggc aacatctcca acctccgcct
     3961 tccctgctcc gccatggccc aggaagtggt gggcgttgag gaagggacca agcaggcgga
     4021 gatcatcggg accatgggga tcatcggctc cgtggtggtg aaccttgccg gggtgaccct
     4081 ggcggccttc gtgggagcat ggatcatcgg cctcttcccg gcctttgtgg ctgacgcctt
     4141 ccggaaattc accgctccgg ccatcttcgg cgccgtgttc ggccagttcg ccatgaggca
     4201 gcccaagctg gctcccttcg ccctggccat ccccctcacc cttctccact tcaaggctcc
     4261 catatggctg gtgatcctgg cgtcggtgtt cggcaccatc gccatttcac ggatattcta
     4321 caaggcaaag ctcattcact gattcttctc ctatgagccg ggggccctcc ggggcccccg
     4381 gctctttacc tttctttacc ctccccgcag attcacgaaa aaaaactgca ccaaatacgg
     4441 accggccatc ccctccatag ggttagaata agggcgaaca gacaaacaaa ggggatggaa
     4501 gcccatgaac aaaagaaatt tcgctcccgc acttcttgta atcctgacag tgctgactct
     4561 ctcgggaagc cttttcgctg aagaaaaaac gatctccctg gaggaatgtc ttgccctggc
     4621 cgaggcaaat caccccgccc tcgaagaggc ccgggcggcc ctggccggac agcaggcgaa
     4681 gctcggccag gtgcgggtga gcaacgccct gaagggaaat ctctcagcgt caggttcgag
     4741 gaattccggc agtgacggat cctataccac gtccttctcc gtgagcaagc tgctctccga
     4801 ctccggcaag aacgccctgg agaggaaaag ccagggcctc tcggtggatg ccgcgtcgga
     4861 atcgcagcgg gagaccgtgc tttccgttcg caccggcgtc aaggacgcct actacgggct
     4921 gctcctggcc atgcgcaagc gcgaccaggc ggaatcggcg gtgaagacct accagcgtca
     4981 cctggacaag gcccgggggt tttacgatgc cggagtcaag gcccggttcg acgtcaccaa
     5041 ggcagaagtg gacctgagca acgcccggat cgacctggtc tccgccgagt cgtccctcga
     5101 aaccgccagg gccgccctgt cgcgggccgt gggcgttccc ctggaccatg cccggcccgt
     5161 atcggacttc cttgccccga gggacatccc cgctgaaaat ttcgccctgg agcaggccct
     5221 cgaaaaccgg ccggacatcc gttcttcccg gctgaaaagc cgggcgggaa aactcggcat
     5281 ttccatcgcc gccaagggca acgccgccac catctccgtc acggggtcag caaagctctc
     5341 gggcacgggc cttcctcccg atgacgatta cagcgtgggg atcacccttt ccgttcccgt
     5401 gttcgacggg ggcctgacgg attacaggat cgccgaggcg cgggcatccg ccgacggcct
     5461 tgacgcttcc ctgaaaaaac tggagcagac cgtccgctac gaggtccggt ccgccctgct
     5521 ttccgtccgg gaggcggaag cccggatccc cgctgccgaa ctcctcgtgc gccaggcgga
     5581 agaaaacctg accctcgccg aggggcggta cgagatgggc gtcgggaacg tcctcgaggt
     5641 cgctgatgcc ctccttgcgt tcaactccgc cagggtcagc catttccagg cgctccacga
     5701 ttacagcacc gccgtttcgg acctcgaaca gactctcgga ggtgaattca aatgaaaaag
     5761 gtttttatcc ttctgatcct catcgccgcc ggtttcgcct atctccggtt ctcccattcc
     5821 gacgggcccc ggtacacgtt ccgcaccgtt cccctggaga agggagatat cacctccacc
     5881 gtaaccgcca caggcaagct ggaagccgtc accgtggtgg aagtggggac ccaggtctcg
     5941 ggtaccctca aggaaattta cgccgacttc aaccagcacg tgaaaaaggg agagctcatc
     6001 gccctcatcg accccgacgt gctcaaggcg aagctcgagg aagcgagggc gaacctcgcc
     6061 gtggcgaggg cctcggcggc cagggcgcgg gccaatcttg cggagagcga ccggaacctc
     6121 aagcggtaca aggagctgtg gaaccgtcag ctcatcgcac gaagcgaact cgacgccgtg
     6181 gaaaccaccc ggctgaccaa cagggcaagc gtccaggaag cggatgccag ggtgctccag
     6241 gtccaggcgt ccctcaggca ggcggagacg aacctggaat acacaaaaat cctctccccg
     6301 gaggacggcg tcatcatctc gagggaggtg aacgtaggcc agaccgtggc cgcgagcctg
     6361 tcggcccccg ttctcttcac catcgccaag gacctttcgg acatgcagat cgagacgtcc
     6421 gtggacgagg ccgacatatc cagggtaaag gaagggcagg aagtggaatt ctccgtggac
     6481 gcctacagcg gaaccacatt caaa
//
LOCUS       DAOTHD010000012         6286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_14717_length_6286_cov_3.445033, whole genome shotgun sequence.
ACCESSION   DAOTHD010000012 DAOTHD010000000
VERSION     DAOTHD010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14717_length_6286_cov_3.445033"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="P5219_00450"
     CDS             <1..566
                     /locus_tag="P5219_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HRX25254.1"
                     /translation="VRELADLGEDALLSLIDSMSVLEGMADMLIIDTGAGIHRNSISF
                     GTASDAVLLVTTPEPTSIRDAYGVLKALAFAAAGKADVTVVVNMAASPEEGEDVADRI
                     RSAASQFLGISPSFGGTVLWDETVPLAVRKRTPLLTAYPDSGASQSLRAVADRMLGLE
                     DAREAPSSGRGVKSFFFRLARGLGLRR"
     gene            570..1247
                     /locus_tag="P5219_00455"
     CDS             570..1247
                     /locus_tag="P5219_00455"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRX25255.1"
                     /translation="MESMTPGMFLNFMNGKVGTKVVLFNEAGLYKGTYPSRVEDVKGD
                     LIGLSHPLLRGALLPVLRNVELKLKVETEGALYQTVVSVARGGPHDGVPVLWVSPVSN
                     AERVQRRYFVRVPCLLKTSFFRLEGEGIKAETEEWTHAVSRDISLGGVGVLMPLPLSE
                     RFSEQDRVLVRLPLQEGTWFLTGKVVRKLRKEDAWEMGFAFEAMPGSVEKPLGAFIRQ
                     QELAGRQ"
     gene            1266..2351
                     /locus_tag="P5219_00460"
     CDS             1266..2351
                     /locus_tag="P5219_00460"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HRX25256.1"
                     /translation="MTESRIRVLVVDDSSFMRKILSDILGTDPRIEVAGTARDGDDAL
                     KKIAELSPDVVTLDVEMPRRDGLSTLEEIMKRNPLPVIMVSSLTQEGAQITLRALSAG
                     AVDFVAKPSGHISLNMRDVSADLVAKVIAASTARVGGRERKGIRSMVPPPALQPAKPR
                     LQPSLPPRAGRPEILAIAASTGGPRALQQVISQLPGSFPVPIVIVQHMPKDFTLSFAK
                     RLDAMSELDVAEGTEGMDLRPGLAVVAPGGYHMIVRRSQAGRLSCGLSDAPPLLSVKP
                     SANIMFLSVADELGGRAVGVILTGMGRDGADGAAALRSKGARIIAESQETCIVYGMPK
                     SAVELGVVDELLPLYSIPEAMIRSVRE"
     gene            2383..4455
                     /locus_tag="P5219_00465"
     CDS             2383..4455
                     /locus_tag="P5219_00465"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HRX25257.1"
                     /translation="MTNMDMSQYLGAFLDEAGDNLKHLDDLILAVEKDQTDMDNIAEI
                     FRSAHTLKGMSSTMGFDRMASLTHAMEDMLDSVRRGTYSLKAKDIDLLFRSLDTLQAM
                     VDSIRNGNGDGSVETDDLVAAMRNAPKEVQAAGMQPEDAPSPGEEKEFSGQELQWISE
                     ASGLGLNVYEVRVSLSPDCLLKAARAYMVVSRLDEMGDIIKTVPPVEALENEEFDHEF
                     IVYAATSRSLEQVREAVARVSEVVGADVREVSRDSAVLPADREAVEPPKEKAPAAGAG
                     KEVKEKTPPPGGSTPPAPAQPQRKSEAAKKANQTVRVDIGRLDKLMNLVGELVIGRAR
                     IERLVQEAHLREFDEPLSQLGRISGDIQELVTKLRMVPVSFIFERFPRLVRDLSKTLG
                     KEIELVMEGQETELDRTVIDEIGDPMVHIIRNSIDHGVEIPAERKKAGKPEKGLIKIS
                     AYQEGSGVIIEVTDDGKGIDASKVRAKALANGTITEEESAAMSDEEVVNLIFLPGFSM
                     AEKVTDVSGRGVGMDAVKTKVESLGGQFDVSTEVGTGTRVFVRLPLTLAIVLALLIKV
                     GDETYALPLENVEETILVKKENVKTVHGTPATLLRGDVLTLCNLAEMLGTSRNGEEAG
                     DEYPVVVVKTGKTRIGFIVDELIGQQDIVIKSLGRFLSKIRGIAGATILGDGNVALIL
                     DVAGLYGR"
     gene            4474..5100
                     /locus_tag="P5219_00470"
     CDS             4474..5100
                     /locus_tag="P5219_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HRX25258.1"
                     /translation="MDLANFSNLQLDAMREVGNIGAGNAATALSIMLARPVDMDVPKA
                     ELVSIYELAEYYGDPLTMVSAVFVRSEGEFTCSLIFIQEEDESRKLVDLLIGQQMGGM
                     DPASVPAEMRDSALTEVGNIILSSFLNAINVLIGGTHSISVPGVAHDMLGSILDVVAS
                     IFGQSGEHALIVDTSLKVENVEEGVSGKVVMLPDPGSLEILLGKLQVL"
     gene            5104..5598
                     /locus_tag="P5219_00475"
     CDS             5104..5598
                     /locus_tag="P5219_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HRX25259.1"
                     /translation="MDKAHHVGMADMVVVSAPESLVTLGLGSCIGLVLFDQAAKVAGM
                     AHIMLPDSRGAGQKALEKPGKFADTAVPALIAEVCRKGAVRSRLKAKIAGGSQMFALP
                     GAQTDFLAVGSRNAKETEAMLQRHGIPLSASDTGGNKGRTVEFSTKTWMLTVKVLGKG
                     TAEI"
     gene            5623..>6286
                     /locus_tag="P5219_00480"
     CDS             5623..>6286
                     /locus_tag="P5219_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25260.1"
                     /translation="MEQERNEVREDLHEDTDLSSAPAPDGGGQDALGPETPGDNPPPS
                     LLDTLPKETADELCALFSCSPEALEVVLRSTSGDPSKILALVRTLTPEYIAVKARFDA
                     RKRGELGGALCFVARGNTGEILDESFWVSGKSLPETFDIDAGWESVRTAIATIGGTPE
                     RTLYTRMLKAAKAIFTPTAINKLFQSRSAKEQILSALGESFSEIVHYDLVYDLKTEIF
                     NKA"
BASE COUNT         1311 a         1882 c         1978 g         1115 t
ORIGIN      
        1 gggtccggga actggccgac ctcggggagg atgccctttt gagtctcatc gacagcatgt
       61 ccgttctcga gggcatggcg gacatgctca taatagatac cggggccgga attcacagga
      121 acagcatttc cttcgggacc gcctcggatg ccgtgctgct ggtgacaacc cccgaaccca
      181 cgtccatccg tgacgcctac ggcgtgctca aggccctggc cttcgctgcg gcgggcaagg
      241 cggacgtgac cgtggtggtg aacatggcag cctctccgga agagggggag gatgtagccg
      301 accgcatacg gagtgcggcg tcccagttcc tcggaatatc tccctccttc ggtggaacgg
      361 tgctctggga cgagactgtc cccctcgcgg tacggaagcg gacgcccctg ctcactgcct
      421 atccggacag cggggcttca caaagtctca gggcagtggc cgaccggatg ctcggtcttg
      481 aggatgcccg ggaggcccct tcttcgggca ggggagtcaa atctttcttt ttccggctcg
      541 cccgcggcct cggcctgagg aggtaagcca tggagagcat gactcccggg atgttcctga
      601 acttcatgaa cggaaaggtc gggacgaagg ttgtcctgtt caacgaggcc gggctgtaca
      661 aggggaccta tccctcacgg gttgaagatg tgaagggaga cctgatcggc ctttcccatc
      721 cccttctccg gggagccctg cttccggtcc tcaggaacgt ggagctgaaa ttaaaggtgg
      781 agacggaggg agccctctac cagaccgttg tttccgttgc caggggaggc ccccatgacg
      841 gtgtccccgt actgtgggta tccccggttt caaatgccga gagagtgcag agacgctatt
      901 ttgtccgggt gccctgcctt ttgaaaacct ccttcttccg cctcgagggt gagggaataa
      961 aggccgaaac agaagaatgg acccacgccg tttccaggga catcagtctc ggaggggtgg
     1021 gcgtcttgat gcccctgccc ctttcggagc gtttctcgga acaggacagg gtgctggtgc
     1081 ggcttcccct gcaggagggc acctggttcc tcacggggaa agtggtgaga aaactcagaa
     1141 aagaggatgc ctgggagatg ggcttcgcgt ttgaagccat gcccgggtcc gtggagaagc
     1201 cgctgggcgc ctttatccgg cagcaggagc tggcgggacg ccaataaatc actgaaggga
     1261 ttgccatgac agagtcgaga atacgtgtac ttgtagtgga tgattcgtcc tttatgagaa
     1321 agatcctgag cgatatcctg ggtacggacc cccgcatcga ggtggccgga acggccaggg
     1381 acggcgatga tgccctgaag aagatcgccg agctttcccc cgacgtggtg accctcgacg
     1441 tggagatgcc ccggagggac gggctctcca ccctcgagga gatcatgaag cggaaccctc
     1501 tccccgtgat catggtgagc agcctgactc aggagggagc ccagatcacc ctgcgtgccc
     1561 tttcggcggg cgccgtggac tttgtcgcaa agccgtccgg gcacatctcc ctcaacatgc
     1621 gggacgtttc agccgaccta gtcgccaagg tcatcgccgc cagcaccgcc cgggtggggg
     1681 gcagggaacg gaagggcatc cgctccatgg ttcccccgcc cgcactccag ccggcgaagc
     1741 cccggcttca gccgtcgctg cctcccaggg ccggccgccc ggaaatcctc gccatcgccg
     1801 cgtccacggg agggccgcgg gccctgcagc aggtgatttc acagcttccg ggaagttttc
     1861 cggtgcccat tgtcatcgtg cagcacatgc ccaaggattt caccctttcc ttcgcaaaac
     1921 ggctcgacgc catgtccgaa cttgacgttg ccgaggggac ggaagggatg gatttgcggc
     1981 cggggctagc cgtagtcgct cccggagggt atcatatgat agtcaggcgg agccaggcgg
     2041 gccggctgag ctgcggcctg tcggacgccc cgcccctgct ctcggtgaag ccgtcggcga
     2101 acatcatgtt cctgagcgtg gcggatgaac tggggggcag ggctgtcggc gtgatcctca
     2161 cgggaatggg acgggacggc gccgacggcg cggccgccct gaggtcgaaa ggggccagga
     2221 tcatcgccga gtcccaggag acatgcatcg tgtacggcat gccgaaatct gcggtggaac
     2281 tcggggtggt ggacgaactt cttcccctgt attccatacc ggaagccatg atccgaagcg
     2341 tgagagaatg aacagacttt acgaagctgg agatgatcag ccatgacgaa tatggatatg
     2401 agccagtacc tgggggcgtt tctcgacgag gcgggagaca atctgaaaca ccttgacgat
     2461 ctcatcctcg ccgttgaaaa ggaccagacg gacatggaca atatcgccga gatcttccgg
     2521 tccgcccaca ccctcaaagg catgtcgtcc accatgggat tcgacaggat ggcttctctc
     2581 acccacgcca tggaggacat gctggattcc gtccgcaggg gaacatacag tctcaaggcg
     2641 aaggatatcg acctcctctt ccggtccctg gacactctcc aggccatggt ggattccatc
     2701 cggaacggaa acggcgacgg atcggtcgaa acggacgacc tcgtagccgc catgagaaat
     2761 gcgccgaaag aggtgcaggc ggccggaatg caaccggagg acgccccttc tccgggagag
     2821 gaaaaagaat tctccgggca ggagcttcag tggatctccg aggcttcggg cctggggctg
     2881 aacgtatacg aagtccgggt ctccctttct ccggactgcc tgctgaaagc cgccagggcc
     2941 tacatggtgg tgagccggct cgatgaaatg ggagacatca tcaagacggt acctccggtg
     3001 gaagctctcg aaaacgagga attcgaccac gaattcatcg tgtatgccgc cacgtcccgc
     3061 tcccttgaac aggtccggga ggccgtggcc agggtcagcg aagtggtcgg cgctgatgtc
     3121 cgcgaggtca gccgcgacag tgcggttctc ccggcggacc gggaagccgt tgagccgccg
     3181 aaagagaaag ctcccgctgc aggtgccggc aaggaagtaa aagagaagac tcctcccccg
     3241 ggcgggagca cccctcccgc tcccgcccag ccccagcgga aatccgaggc tgccaaaaaa
     3301 gccaaccaga ccgtcagggt cgacatcgga cggctggaca agctcatgaa cctcgtggga
     3361 gaactcgtga tcgggcgggc acgaatcgag cgcctggtcc aggaggccca tctcagggag
     3421 tttgacgaac ccctctccca gctcggaaga atttccgggg atatccagga gcttgtgacc
     3481 aagctccgca tggtacccgt ctccttcatt ttcgaacggt tcccccgcct cgtgcgggac
     3541 ctctccaaga ccctcggcaa ggaaatcgag ctggtcatgg agggacagga aacggaactg
     3601 gaccgcaccg tcatcgacga gatcggcgac cccatggtgc acatcatacg gaactccatc
     3661 gaccacggcg tcgaaatccc cgcggaacgg aaaaaggcag gcaagccgga aaagggcctc
     3721 ataaagattt cggcctacca ggaaggcagc ggcgtcatca tcgaggtcac cgacgacggg
     3781 aaaggcatcg acgcctccaa ggtcagggcg aaagccctgg cgaacggcac catcaccgag
     3841 gaggagtcgg cggccatgtc cgacgaggaa gtggtgaacc tcattttcct cccgggcttc
     3901 agcatggcgg agaaggtcac cgacgtgtcg ggacggggcg tcggcatgga tgccgtgaag
     3961 accaaggtcg agtccctcgg cggccagttc gatgtctcca cggaagtagg gaccgggacc
     4021 agggtcttcg tccgcctgcc cctcaccctc gccatcgttc tcgccctgct catcaaggtc
     4081 ggggacgaga cctacgccct ccccctggag aacgtggaag agaccatcct cgtcaagaaa
     4141 gagaacgtga agaccgtcca cggcacgcct gccacccttc ttcggggtga cgtgctcacc
     4201 ctgtgcaacc tggcggaaat gctgggcaca tcgaggaacg gagaggaggc aggcgacgag
     4261 taccccgtgg tggtggtcaa gaccggaaag acccggatag gcttcatcgt ggacgaactc
     4321 attggccagc aggatatcgt catcaagtcc ctcggccgct tcctctcgaa aatcaggggg
     4381 attgccgggg caacaatcct cggggacggg aacgtggccc tcatcctcga cgtggccggc
     4441 ctgtacggca ggtagggaag gagccgcccc ttcatggatc tcgcaaactt cagcaatctg
     4501 cagctcgacg ccatgcggga agtgggcaac atcggcgcgg ggaacgccgc cacggcgctc
     4561 tccatcatgc tcgcccgccc ggtggacatg gatgtcccga aggcggaact ggtgtccatc
     4621 tatgaactgg cggaatacta cggcgatccc ctgaccatgg tgtctgcggt gtttgtccgg
     4681 tccgaagggg agttcacctg cagcctcatc ttcattcagg aagaggacga gagcagaaag
     4741 ctcgtcgacc tgctcatcgg ccagcagatg ggcggaatgg accccgcctc ggttcccgcg
     4801 gagatgcggg acagtgcgct gacggaagtg ggcaacatca tcctcagctc cttcctgaac
     4861 gccatcaacg ttctcattgg gggaacccac agcatctcgg tccccggagt cgcccacgac
     4921 atgctcggtt ccattctcga cgtagtggct tccatcttcg gccagtccgg cgagcatgcc
     4981 ctcatcgtgg atacctccct gaaggtggag aacgtagagg aaggagtttc gggaaaagtg
     5041 gtcatgcttc ccgatcccgg ctctctcgag attcttctcg gaaagctgca ggtgctctaa
     5101 agaatggaca aggcgcatca cgtagggatg gccgatatgg tggtcgtctc agctcccgag
     5161 tccctcgtga ccctcgggct ggggtcgtgc atcgggctcg tgctctttga ccaggccgca
     5221 aaggtggcgg gcatggctca tatcatgctt cccgacagcc gcggcgccgg gcagaaggca
     5281 ttggaaaagc cgggaaaatt cgccgacacc gcggtgcctg ccctgattgc cgaggtctgc
     5341 agaaaagggg ccgtccggtc aaggctcaag gccaagatag cgggcggatc ccagatgttt
     5401 gcccttcccg gggcgcagac agattttctt gcggtaggca gcagaaatgc caaggagacc
     5461 gaggccatgc tccagcggca cggaattccc ctttccgcct ccgatacggg ggggaacaag
     5521 gggagaaccg tggaattttc aacgaagacg tggatgctca cggtaaaggt gctggggaag
     5581 ggaacggcgg agatctgaca taatgccctg acaggaggag ccatggaaca ggaacggaat
     5641 gaagttcggg aagacctgca cgaggatacc gatctctctt ccgcccccgc gccggacggc
     5701 ggaggacagg atgcccttgg accggagact ccgggagaca atcctccccc ttcgcttctt
     5761 gataccctgc cgaaagagac ggcggacgaa ctctgcgccc ttttctcctg ttctccggaa
     5821 gcccttgaag tcgtcctcag gagcacctcg ggggatcctt cgaagattct ggccctggtc
     5881 aggacgctga cccctgaata tatcgccgtc aaggcccggt tcgacgcccg gaagagaggg
     5941 gagctcggcg gcgccctgtg tttcgtggcc cggggaaaca ccggcgagat cctcgatgaa
     6001 agtttctggg tcagcggaaa aagcctgccg gaaaccttcg atatcgacgc cggctgggag
     6061 tcagtccgaa cggccatagc caccatcggc ggcactccgg aacggaccct ctatacccgc
     6121 atgctcaagg ccgcgaaggc aatctttacc cccaccgcaa tcaacaagct ctttcagtcc
     6181 aggagcgcga aggaacaaat actctccgct ctcggcgaat ccttctcaga aatcgtccat
     6241 tacgatcttg tctatgacct caagacggaa atcttcaaca aggcca
//
LOCUS       DAOTHD010000013         5913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_15993_length_5913_cov_3.629908, whole genome shotgun sequence.
ACCESSION   DAOTHD010000013 DAOTHD010000000
VERSION     DAOTHD010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15993_length_5913_cov_3.629908"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1303
                     /locus_tag="P5219_00485"
     CDS             <1..1303
                     /locus_tag="P5219_00485"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRX25261.1"
                     /translation="CAILTDREIPFPVPQLLTTDVRRDMGRLASIVYGCPSSRMKMFA
                     VTGTNGKSTTTWMIRHILQSLGIRTGLFGTIVYNDGAVEKDAGRTTPESYEIQRSLAS
                     MVKNGCGACVMEASSHGLSLGRLEGCSFDGAVFTNLSEEHLDYHKTLDEYFQAKSRLF
                     SQYMKDGWHGASNMDDLHGRMLLSAFPGKLIPFGLGENSAEGVRGSILSATPSGNEFS
                     VRLPGRQKDLKVFLPLPGRFNVYNALGSIAILQPFVENPERTAAALRTMPQVPGRLER
                     YYLSSGVCVIIDFAHTPAALGNVLSELRNMCACRLFAVFGHGGERFQPNRHSLGMTAA
                     RLCDRIIVTMDNPRSEDPGDIADQICEGIRSSEREPETEIILDRKAAIRRALDEARAG
                     DVVAVTGKGPEKYLIIGNQTIPYSDRETVLDWVQERGLTWE"
     gene            1300..2682
                     /gene="murF"
                     /locus_tag="P5219_00490"
     CDS             1300..2682
                     /gene="murF"
                     /locus_tag="P5219_00490"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRX25262.1"
                     /translation="MNTFSFIAELAGSKLRGPDGVFSGRLLADSRLVRKGDAFVAFRG
                     ENTDGHTFIADAIARGASLIICEEDRDIPSPVASVKVSDTYKALPAMAEKRLSRHGKK
                     LHVIAITGSVGKTTTRDYLVRCLKTSMKVHSAEHSYNTLIGCSMTILALPEEAEVLVL
                     EMGTNHPGEIGEMVRFFPPTISVITEVAPAHLEGFGSVEGVLEAKMEIAGSSALQSFF
                     FNGDNPLLVRAASRLPAGIAGFSAGTGECDYRILAPVFSIEKGIPELSFSLEIPGGDI
                     LAVQSGAFGKHAAYPMAFAIGISTRLGVPGEKAAAALKETHSLEGRGLIRTLNSGACL
                     IDDSYNANPASMSAVLRAAGEMETRRTYAVVGEMLELGERGDDFHRDLLPLLRSFDTV
                     WLVGKTWERIAPFDGQAETRLVLWSDSLQKLGDCLGKELGEGDAIVIKGSHGNRLDRL
                     VDILCREGAS"
     gene            2679..3635
                     /locus_tag="P5219_00495"
     CDS             2679..3635
                     /locus_tag="P5219_00495"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRX25263.1"
                     /translation="MKFIFIFSVVIILEIFLQSRWIHALNRRQITLAQKAYGIQSHME
                     TKSSTPSMGGIVFIATALASILLAGWFSDAAPGELTLFWFFPILSAAVGFADDWIKFS
                     KKSSEGFPSLYKLAFQVFIVFPWVMWISLSRGISIWPGILISPWLAVPLAAFLSIGML
                     NAVNVTDGLDGLAAGACVISMAGALLWLPLRGSAFYGAVTGLGLCAAFLWHNAYPAKV
                     FMGDVGSHFIAGLIVSTAIHGECLVALIPLGFVYGLEILSVSAQLTAIYGFKKKVFLM
                     SPVHHHFELLGWRENHIVTRFWLVHGVGLAITAVLFSSYFGN"
     gene            3665..5035
                     /gene="murD"
                     /locus_tag="P5219_00500"
     CDS             3665..5035
                     /gene="murD"
                     /locus_tag="P5219_00500"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRX25264.1"
                     /translation="MEPGNNLQGKKITIIGSGVSGKGLASFAAGLGASVFVTERKHLD
                     EEARAMFRKKGIAWEEDGHSVRACDADMVVAGSGIPPAAPVVIEARKRGIPVTGELDF
                     LAPFLRGKVLGVTGSNGKSTTTALLGHMLTRKGFAVSVAGNIGTSLADSALRNWDYIV
                     VELSSFQLHWNYRLSCSVAVVTNLAPDHIDWHGSYEEYIRAKARIISTQRECGIAVVQ
                     ERDRTVLGSDLADDRFVCFSWKEDFSGRCKKAILADERTRSVYFRTGNDDEKLFGFDD
                     LPLIGRHNIENASMAAGVLRLLGEKDIPGLFTGFCGLPHRCEIVADIGGVLFIDDSKG
                     TNVASTCTALTSIEGPKAIILGGQGKGEDYAPLAETVRKEALAAVVMGEEREKIVSAL
                     KKAGYGAIIEAEGMEDAVKKAASALPGKGVVLLSPACTSWDMYPNYKKRGEHFKSIVL
                     SLEGSS"
     gene            5032..>5913
                     /locus_tag="P5219_00505"
     CDS             5032..>5913
                     /locus_tag="P5219_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRX25265.1"
                     /translation="MREESMLSAEVPAGRPQGDPFLWIIPLLLAIFGILMIFSLTSHT
                     SLEEYGSPFTLGMKQVHWLLVGIAGMLVMYMIPLSFWKKASGTIWLFSLVLLVLTLIP
                     GIGIKAGGARRWIGFAGLRFQPIEFLNLALAVHLSRLLTTSEKRGLSAFLTITVPVLF
                     LSVIPLLFQPNMGGTILIFTLSMAIHVQSRGWSWPFIAGAAGFPFFFMFIIREGYRLR
                     RYIAFLDPWEQPMDSGFQVIQGLVAFSNGGLFGVGVGKGLQKLNYLPAAHTDYIFATV
                     GEEFGFIGTFFILFFFLF"
BASE COUNT         1292 a         1648 c         1598 g         1375 t
ORIGIN      
        1 atgcgccatc ctgaccgaca gggagattcc cttccctgtc ccccagctgc tgaccaccga
       61 tgtccgaagg gacatgggcc gcctcgcttc gatcgtgtac gggtgcccct cctcaaggat
      121 gaaaatgttc gccgttaccg gaaccaacgg aaaaagcacc acgacctgga tgatacggca
      181 tatccttcaa tccctgggaa tcaggaccgg ccttttcgga accattgtat ataatgacgg
      241 ggccgttgag aaagatgccg gaagaacaac cccggaaagc tatgaaatcc agcgttccct
      301 tgcatccatg gtgaaaaacg gatgcggggc ctgcgtcatg gaagcgtcat cccacggact
      361 gtctctcgga agactggaag gctgctcctt cgacggtgcc gtcttcacga atctcagcga
      421 agagcacctg gattatcaca agaccctgga cgagtatttt caggcgaaaa gccgtctttt
      481 ttcacagtat atgaaggacg gctggcacgg agcttccaat atggatgacc ttcatggaag
      541 gatgctcctc tccgcctttc cgggaaaact catccctttc ggtcttggag agaacagcgc
      601 cgaaggcgtc aggggaagta ttctttccgc aacaccctca ggaaatgaat tttctgtccg
      661 cctgcccggc aggcagaaag atctgaaggt ttttcttcct cttccggggc ggttcaatgt
      721 gtataatgca ctgggttcga tcgctattct tcagccgttt gtggagaatc ccgaaaggac
      781 agccgccgcg ctcagaacca tgccccaggt tccgggaaga ctggagcggt attatctttc
      841 cagcggagtc tgcgtgataa ttgatttcgc ccatacccct gcggccttgg gaaatgtact
      901 gtccgaactc cggaacatgt gcgcctgcag acttttcgcc gttttcggcc acggagggga
      961 gcgattccag ccgaaccgcc attctctcgg catgaccgcc gcccggctct gcgacaggat
     1021 catcgtcacc atggacaacc cgaggagcga agatcccggc gatattgcgg atcagatttg
     1081 cgaaggcatc cgttcttcag aaagggaacc tgaaacggaa attatcctcg acagaaaagc
     1141 ggcaatcaga agagcccttg atgaagcacg ggccggagat gtggttgccg tcaccgggaa
     1201 agggccggaa aaatatctca tcatcggaaa tcagaccata ccctattcag acagagagac
     1261 tgtccttgac tgggttcagg agagaggctt gacgtgggaa tgaacacatt ttcttttatc
     1321 gctgaactcg caggttccaa actgcggggc cccgacggtg tcttttccgg cagactgctc
     1381 gccgacagca ggctggtccg gaaaggagac gcctttgtcg cattccgggg agaaaacaca
     1441 gacgggcaca cctttatagc cgatgccata gcccgcgggg catccctcat catctgcgag
     1501 gaagaccggg acattccctc cccggtcgcc tcggtaaagg tcagcgatac ctacaaggcc
     1561 cttcccgcca tggcggaaaa gcggctttcc cgccatggta aaaaactgca cgtcatagca
     1621 attaccggaa gcgtaggcaa aacaaccacg agggactatc ttgtgagatg tctgaagact
     1681 tccatgaagg tccattcagc ggaacacagc tacaatactc tcatcgggtg ctccatgacc
     1741 atccttgccc ttccggaaga ggcagaggtt ctcgttcttg aaatgggcac caaccacccc
     1801 ggagaaatcg gggaaatggt ccgcttcttc cctcccacca tttccgtgat cactgaagta
     1861 gcgcccgccc acctcgaggg gttcggatcg gtggagggtg tgctcgaggc caaaatggag
     1921 atcgccggat catctgctct ccagtccttt ttcttcaacg gagacaatcc tcttcttgtc
     1981 agggcggctt cacggcttcc ggcaggaatt gccgggttct ccgcaggaac gggggagtgc
     2041 gattaccgca tacttgcccc ggttttttcc atcgaaaaag gtatccctga actttccttt
     2101 tcccttgaaa tccccggggg ggatatcctc gccgtacaat ccggggcttt cggaaaacac
     2161 gcagcctacc cgatggcatt tgcaatcggc ataagcaccc ggctcggagt tccaggagag
     2221 aaagccgctg cagctctaaa agagacccat tcccttgaag gacggggact catcagaacc
     2281 ctcaattcag gagcatgcct catcgacgat tcctacaatg ctaatcccgc ctccatgtcg
     2341 gccgttttga gggcagcagg cgaaatggag acgcgccgca cgtatgcggt cgtcggtgaa
     2401 atgcttgaac tcggggagag aggcgatgat ttccacaggg acctgctgcc ccttctccgt
     2461 tctttcgata ccgtctggct tgtgggcaaa acctgggaaa ggattgcccc gttcgacggt
     2521 caggcagaaa ctcgccttgt cctctggagc gacagtctcc aaaagctggg agactgtctc
     2581 gggaaggaac tgggtgaagg tgacgccatc gtaatcaagg gatcccacgg caaccggctc
     2641 gacaggcttg tagatattct ctgccgggag ggggcatcat gaaattcatt tttatttttt
     2701 cagtggtcat tattctcgaa attttccttc agtccaggtg gattcacgcc ctgaaccgcc
     2761 ggcagataac cctcgcccag aaggcctacg gcatccagag ccacatggag accaagtcat
     2821 ccactccttc catgggaggc atcgtgttca tcgccacggc tcttgcatcc atcctcctgg
     2881 ccggatggtt ctcagacgct gctccgggtg aactgacact tttctggttt tttcccattc
     2941 tttccgccgc agtgggattc gcggacgact ggataaaatt cagcaaaaag tcaagtgaag
     3001 ggtttccaag cctctacaag ctcgcctttc aggttttcat cgtctttccc tgggtaatgt
     3061 ggatttccct ttcccgggga attagcatct ggcccgggat ccttatttct ccctggcttg
     3121 ccgtccccct tgcagccttt ctttccatag gaatgctcaa tgcggtcaat gtgaccgacg
     3181 gccttgacgg cctcgctgca ggggcgtgtg tcatttccat ggcaggcgca cttctctggc
     3241 ttcctctccg gggctccgca ttttacggcg ccgtcaccgg cctcggcctc tgtgccgctt
     3301 ttctctggca caacgcctat ccggcaaaag tcttcatggg ggatgtggga tcccatttca
     3361 tagcaggtct catcgtcagc actgccatac acggcgaatg ccttgtcgcc ctcattcctc
     3421 tcggttttgt ctacgggctt gagatccttt cggtttccgc gcagctgacg gcgatttacg
     3481 gattcaaaaa aaaggttttt ctcatgagcc ctgttcacca ccatttcgag ctgctgggct
     3541 ggagagaaaa ccatatcgtc accaggttct ggcttgttca cggagtcggc cttgcaatta
     3601 cggcggtcct cttttcgtca tatttcggga actgacgttc cccggtcttt agaggtgtat
     3661 agccatggaa ccagggaata atcttcaggg gaaaaaaatc accatcatcg gatccggagt
     3721 cagcggcaaa ggcctggcct cattcgctgc cgggctgggc gcatcggttt tcgtcaccga
     3781 gagaaaacac ctggatgaag aggcccgggc catgttcagg aaaaagggga ttgcctggga
     3841 ggaagacggc cattcagtcc gggcctgcga tgccgacatg gtcgtggcag gttcgggtat
     3901 tcctcctgcg gctccggtgg ttatcgaggc acgaaaaagg gggataccgg tcacgggaga
     3961 actcgacttt cttgcccctt ttctccgggg gaaagtgctg ggggtaacgg gaagcaacgg
     4021 caaaagcacc accaccgcac tcctcgggca tatgctcacc agaaaaggtt ttgctgtttc
     4081 tgtcgccgga aacatcggga cttcccttgc cgactccgct ctcaggaact gggattatat
     4141 cgtcgtggaa ctgagcagtt tccagctcca ctggaattac cgcctgtcct gcagtgtggc
     4201 tgtagtgacc aacctggcac cggaccacat tgactggcat ggaagctacg aagaatatat
     4261 ccgggcaaag gccaggatca tcagcaccca aagggagtgc gggatagcgg tcgtccagga
     4321 gagagacagg actgtccttg gttcggacct tgctgatgac cggttcgtgt gcttctcgtg
     4381 gaaagaggat ttttccggcc ggtgcaaaaa ggcgatcctt gccgacgaaa gaaccaggtc
     4441 cgtgtatttc agaaccggga atgacgatga gaaacttttc ggttttgatg accttcccct
     4501 cattggaagg cacaacatcg aaaacgcttc catggcggcg ggcgttcttc gtctcctcgg
     4561 agagaaggac atacccggcc ttttcacagg attctgcgga ctgccccaca ggtgcgaaat
     4621 agtcgcggac atcggggggg ttctcttcat tgacgactcc aagggaacga acgtagcctc
     4681 cacctgtacc gccctgacct ccattgaagg tccgaaggcc atcatccttg gggggcaggg
     4741 aaaaggcgaa gattacgccc ccctggcgga aacggtgcgg aaagaagctc tcgcggcagt
     4801 ggtgatgggg gaggagcggg aaaaaatcgt cagcgctctc aagaaagccg ggtacggagc
     4861 gatcattgaa gccgagggga tggaagatgc cgtgaaaaag gccgcttcag cccttcccgg
     4921 gaaaggcgtg gtcctccttt cccctgcatg caccagctgg gacatgtatc cgaattacaa
     4981 gaaaagggga gaacacttca agagtatcgt tctgtccctg gagggaagct catgagagaa
     5041 gaatctatgc tttccgcaga agttcctgcc ggacgccccc agggtgatcc tttcctctgg
     5101 atcatcccgc tcctgcttgc tattttcggc atcctcatga ttttctccct tacgtcccat
     5161 acatcccttg aagagtacgg ttcacccttc accctcggga tgaaacaggt acactggctg
     5221 ctggtcggaa tagcggggat gctggtgatg tacatgatcc ccctttcttt ctggaaaaaa
     5281 gccagtggga caatctggct cttttccctt gttctcctcg ttctgactct tattcccggc
     5341 ataggcatca aggctggtgg tgcaaggaga tggataggct ttgccgggct ccgctttcag
     5401 cccatagaat tcctgaacct ggccctggcg gtacatcttt caaggctatt gaccacatcg
     5461 gaaaaaagag gactatcggc gtttctcacc atcacggtcc ccgtcctctt tctttcggtg
     5521 attcccctcc tcttccagcc gaacatgggt ggaaccatcc tcattttcac tttatccatg
     5581 gcgattcatg tccagtccag gggatggagc tggcctttca tcgccggtgc tgcaggattt
     5641 ccgttttttt tcatgtttat catcagggaa ggataccgtc tcagaagata cattgctttt
     5701 ctcgatccgt gggagcagcc catggacagc ggattccagg ttatccaggg actggtggcc
     5761 ttctccaacg gcggtctctt cggcgtagga gtggggaagg gacttcagaa actgaattac
     5821 cttcccgcag cccataccga ctatattttc gccaccgtgg gagaagagtt cggctttatc
     5881 ggcacattct ttatcctctt ctttttcctg ttt
//
LOCUS       DAOTHD010000014         5835 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_16247_length_5835_cov_3.768858, whole genome shotgun sequence.
ACCESSION   DAOTHD010000014 DAOTHD010000000
VERSION     DAOTHD010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16247_length_5835_cov_3.768858"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="P5219_00510"
     CDS             complement(<1..366)
                     /locus_tag="P5219_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRX25266.1"
                     /translation="MTTRKKTFLFLPALLAAAFLLTASPSSASETLNVYTIWSERYAT
                     AVFDAFTRDTGVHVNFLRFPSGEVLARLVAEKENPQVDVFFGGIADAFEAGKKEGIFE
                     TYVPSGAEAIPQRFRDPEGF"
     gene            610..1125
                     /locus_tag="P5219_00515"
     CDS             610..1125
                     /locus_tag="P5219_00515"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRX25267.1"
                     /translation="MAKTFMSATKSEGGEAAEFDYILSPSVAGFLYPLFVPALVFLAP
                     FILVSIFPNLLTGMGLNVGKWTLLMWGVLLTAANIGIQEIRRRSKKLKLHIDYLVFSQ
                     GIFFTSMEKIFIQDIKTTELTKNIFERIFNLGTIKIATAGTDGYEVELKGYSNPDEIV
                     KYIAVHRKEGE"
     gene            1163..1426
                     /locus_tag="P5219_00520"
     CDS             1163..1426
                     /locus_tag="P5219_00520"
                     /inference="COORDINATES: protein motif:HMM:NF018644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HRX25268.1"
                     /translation="MTEALLPAFLGVNILAFVLMGWDKLMAKASARRIPENTLLLAAL
                     FGGGAGAFLGMKLFRHKTRHPRFFLVLPVVAALQVGALVYFAM"
     gene            1482..3344
                     /locus_tag="P5219_00525"
     CDS             1482..3344
                     /locus_tag="P5219_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25269.1"
                     /translation="MAGGAIRLQKKSTRGSLLSRQWGAPFLAAVLSLVFVLFRPPAAE
                     GGPWAPIIDAAAGPGGVLYVLTSEKGGLFVSEDGGRRWKNLGAGLPSSLLFSLDVSTG
                     GRLFLASFDELLTSGNGGSAWKSMKGGGGVKRFFPTSGDTLLAVFWDSGIHWASSGNG
                     SFEKSGGEDGSFQVLDVLDEGHGRLRAAAFGRGALFSGDGGRTWSPDDPGIENVFPLA
                     LARSSATGTVFAGTLEGGVFRKEEGSPWSRSSEGLPPFCTVQALAAGEQGALWAGTHK
                     QGLFVSSDDGRTWNLFPAGMEKEMSVTVLVPFGKGVLAGTSSGELYLADGEKGTVTEL
                     LPSDPVVGFARIDGSLLCLSRSGKVYVSSDGGAGWTPRGSAGGKALFLAAVPGGRLFA
                     GTEKDLLFSRDGGFTWTSIPLPEGTVFTSLAGTAGGDLFAGSAGSGLFRSSDGYGWQR
                     VGDVPGDLVHSLRSHNGTVIAAGTDAGFSVSLDGGLSWKNGDVIYGIFSLAFDGQGRL
                     WGASRTGLWHYSLPEGEITGAQTAGFDWSPLAYFTDLFQGEGNGLVALLGEKAVRLLP
                     GKAAGSFTLGETNLSNVRVLSSFRDAGGALLLGTERGLFRSTDGGASWSEFGLP"
     gene            3378..4586
                     /locus_tag="P5219_00530"
     CDS             3378..4586
                     /locus_tag="P5219_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25270.1"
                     /translation="MRELYRRLAVLLLLFAALVSFFSVRNGFVRLYVADMGREYRQEY
                     SPSQGLFGAQAAGGRFIRSITPFRTIAGFIDSKTDGHLLTVEGDPWPEIFSGAAAPAG
                     SGATAWREAGGVYRKSLYYHREEEPFPALIAGLEDKGETFSYLKLRTGEGPRFLAMVL
                     EGPGTALDNGVPAPLARPFRSLALVPLCLGAAVYFFLPKRKTPPGAIVYPKWSAVFLP
                     DLLSYIMSGFFLGLPMVLCVHIFDRAGFLDFRFGSAWFTLVFWAIGLIFASILWWTSK
                     YASFALQILPGGLFLRTLGRERAIDFGDIVSAGFVDYRPPKWLRTLLFIAGLVNWRMM
                     GQALLLSGRTDWGIEFFFRDGGSERFLCSNIPGAGRLFDALRLHKIPLSEELQRAAEG
                     TVGPGAEEES"
     gene            4613..5410
                     /locus_tag="P5219_00535"
     CDS             4613..5410
                     /locus_tag="P5219_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25271.1"
                     /translation="MRTVLRRIFKGPSARRTCRFLGLAGLLVCQAAVKPGGAEPSVPP
                     SNEWIHNRIAVLEQALSLRNEAHRMEADGNVPGAMEACWKSFVLYPDSRLEKKVFSFR
                     PDLQPLKDRIAYLESLRAARQAGPATASGPAAAPQAPGGDSAGRDNPDWRTYPDLPAE
                     KKAIGEVFSSFRSALKAGDTKRAAACVDESRRETYAALFENRPEAMPSFAALLDAAEM
                     SFLSAPESADPGTTSTLRTSEYAVDVGGFTFYVRWVKVDGKWMLFDF"
     gene            5436..>5835
                     /locus_tag="P5219_00540"
     CDS             5436..>5835
                     /locus_tag="P5219_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25272.1"
                     /translation="MTMHDRILKKGTAILAGLLLFAAVSPAHGWSQHPHRQINLEAVK
                     LFLSQAGGKEKFSMGPFSPKGLSEPLRGVAVTSSSLLASGFVSAEAAFSAQSWIINGG
                     DWADEPHLYSSVRHFYDPLSLSGSAWLTDQS"
BASE COUNT         1053 a         1725 c         1811 g         1246 t
ORIGIN      
        1 gaagccttca gggtcccgga acctctgcgg tatggcttcg gctcctgaag gcacataggt
       61 ttcgaaaatg ccctcctttt ttcccgcttc gaaggcgtcg gcgatgcccc cgaaaaagac
      121 gtccacctgg gggttttcct tttcagccac cagccgggcg agcacttctc ccgacggaaa
      181 gcggagaaaa ttcacatgca ctcccgtgtc ccgggtgaag gcgtcgaata cggccgtggc
      241 atagcgctcg gaccagatgg tatatacgtt gagggtttcc gaagcggaag aaggggacgc
      301 cgtcagaagg aatgcggcgg ccaggagcgc cggaagaaaa agaaacgttt tttttctggt
      361 tgtcattgca gtcaacctcc ccgaatatgt gtaaagcaaa tggtacagcc tgaaacaaac
      421 ccgctccata tccagtccgg gccaaaatga acctcttctg gatcgcctct gtctttttct
      481 gaaaaaagag tcctgctcat ggtagaatgt tcgccaggga caccgttctt tcaactttcc
      541 ctttcccggg aggggatggg gtatgctcca tgggctgtgg agcaatctct tgagaaggag
      601 tcgatcaaaa tggcgaaaac gttcatgtct gcgacaaaat cagaaggcgg ggaagcggcg
      661 gagtttgact atattctctc tccctcggta gcgggattcc tttatcctct cttcgtgccg
      721 gccctcgtgt ttcttgcccc ctttatcctg gtgagcattt tccccaacct gctgaccggc
      781 atggggctca acgtggggaa atggaccctc ctcatgtggg gagttctcct gaccgcagca
      841 aatatcggaa tccaggaaat ccgccgccgg agcaaaaaac tgaagctcca catagattac
      901 cttgtctttt cgcaggggat attcttcacc tccatggaga aaattttcat ccaggacatc
      961 aagaccaccg agctgacaaa gaatatattt gaacggatct tcaaccttgg aaccataaag
     1021 atagccactg caggcaccga cggttatgaa gtcgaactca agggatattc caaccctgac
     1081 gagatcgtga aatacatagc ggtccacagg aaggaagggg aataggcttc atgatccccg
     1141 ccccggagag ggacgtgtcc tgatgacgga ggcccttctt cccgcgtttc tcggggtgaa
     1201 cattctcgct tttgtcctta tggggtggga caaactgatg gctaaagcgt cggccaggcg
     1261 cattcccgaa aataccctgc tcctcgccgc cctgttcggc ggaggagcgg gggcttttct
     1321 cggaatgaag ctgttccgcc ataagacgcg gcatccccgt ttttttctcg tgcttccggt
     1381 ggtggcggcg ctccaggtgg gagcgttggt ctacttcgcg atgtgacctt tcttcggggc
     1441 cgtctcccga cggtcccgca tgccggggag gtggggcggc aatggctgga ggagcgatcc
     1501 ggcttcaaaa gaagagtacc cgcggcagcc tcctttcccg gcaatgggga gctccttttc
     1561 tcgcagccgt tctgtctctt gtttttgtcc tgttccggcc tcccgccgcc gaaggggggc
     1621 catgggcacc cattatcgat gccgccgccg ggccgggtgg tgtcctgtat gttctcacct
     1681 ccgaaaaggg aggcctcttt gtctcggaag acggcggcag gcgatggaaa aacctcggcg
     1741 ccggtcttcc ctcttccctt ctgttttccc ttgatgtttc cactgggggg cgtctctttc
     1801 tcgcgtcctt cgacgaactt ctcacgtccg gcaacggagg ctccgcctgg aaatccatga
     1861 agggcggcgg cggagtgaaa cgttttttcc ccacatcggg agacaccctc ctggctgttt
     1921 tctgggattc aggcatccac tgggcttcct ccggaaacgg ctcttttgaa aaatccggag
     1981 gagaagacgg atccttccag gttcttgacg tactggacga agggcatggc aggctccggg
     2041 cagccgcttt cggaagaggg gcactttttt caggcgacgg gggaaggacc tggagccccg
     2101 acgatccggg aatcgaaaat gttttcccgc tggctttggc ccgcagttca gcaacaggga
     2161 cggtatttgc cggaacgctg gagggcggcg tcttccggaa ggaggaaggt tccccgtgga
     2221 gccgttcatc ggaaggcctt ccgcctttct gcacggtcca ggcgctggcc gccggcgaac
     2281 agggcgcgct ctgggcggga acccacaagc aggggctctt tgtctcctcc gacgacggaa
     2341 gaacgtggaa cctctttcct gccgggatgg aaaaagagat gtccgtcacc gtcctcgttc
     2401 cattcggaaa gggggtcctc gcgggaacct cctccgggga actgtacctg gccgacgggg
     2461 aaaaggggac cgtcacggaa ctgctcccct ccgaccctgt cgttggtttt gcccggattg
     2521 acggcagtct gctctgcctt tcacggtccg ggaaagtgta cgtttcttcg gacgggggag
     2581 caggatggac gccccgggga agcgcaggag gaaaagcgct cttcctcgcc gctgtccccg
     2641 gaggccggct gttcgccgga acggagaagg atcttctctt ttcccgggac ggagggttca
     2701 catggacctc cattcccctt ccggaaggaa cggtattcac gtccctggcc ggaacggcag
     2761 gcggagacct cttcgcaggc tcggcgggga gcggcctgtt tcgttcttcc gacggatacg
     2821 gctggcagag agtcggcgac gtgccgggcg acctggtcca ttccctgcgg tcccataacg
     2881 gaacggtcat agcggcagga acggacgctg gtttttccgt ctccctggac ggcggtcttt
     2941 catggaagaa cggagatgtg atttacggga ttttctccct ggcctttgac gggcagggac
     3001 ggctgtgggg ggcctcacgg accgggctgt ggcactattc gctccccgaa ggggagatca
     3061 ccggggcgca gacggcaggt ttcgactggt cgccccttgc gtatttcact gacctttttc
     3121 agggagaagg gaacggactg gtcgccctcc tgggggaaaa ggcggtacgc ctccttcccg
     3181 gaaaggccgc ggggtccttc actctcggag agacgaacct ttccaacgtc agggttctgt
     3241 cctccttccg ggacgctggg ggggccctgc ttctggggac ggagcggggg cttttccgtt
     3301 cgacggacgg gggagcctca tggtcggaat tcggcctgcc ctgaacgggt gcggggagaa
     3361 gcgggggtga tcctctcatg agagaactct atcgaaggct ggcggtgctc ctgctgctct
     3421 ttgcggccct tgtctccttc ttttccgtgc ggaacggctt cgtccgcctg tacgtcgccg
     3481 acatgggcag ggaataccgc caggaataca gcccgtccca gggactgttc ggagcccagg
     3541 ccgcgggagg ccggttcatc cggtccatca cgcccttccg taccattgcc ggcttcatcg
     3601 attcgaaaac tgacgggcac cttctgaccg tggagggaga cccgtggccc gaaatattct
     3661 ccggggccgc ggcaccggcg ggctccggcg cgacagcctg gagggaagcc gggggagtct
     3721 accggaaaag cctgtactac catcgggaag aggagccctt tcccgctctc atcgcagggc
     3781 tggaggacaa gggggagact ttctcgtacc tgaagcttcg caccggtgaa ggtccccgct
     3841 ttctcgccat ggtcctcgag ggcccgggga cggcgctcga taacggggtc ccggcgccgc
     3901 tggcacggcc gttccgctcg ctggccctgg tgcccctgtg tctcggcgct gcggtctatt
     3961 tcttcctccc gaaaagaaaa acccctcccg gggccatcgt gtaccccaaa tggtccgccg
     4021 ttttccttcc ggatctgctg tcctatatca tgtcgggatt ctttctgggg cttcccatgg
     4081 tgctctgcgt tcacatcttc gacagggccg gtttcctcga cttccggttc ggcagcgcct
     4141 ggttcaccct tgtattctgg gcaatcggcc tgatcttcgc ttccatcctc tggtggacgt
     4201 cgaaatacgc ctccttcgcc cttcagattc tgccgggcgg actgtttctg aggaccctgg
     4261 ggcgtgaacg ggccatcgac ttcggtgaca ttgtctccgc cggtttcgtg gattacaggc
     4321 cgccgaaatg gctccggacc ctcctgttca tcgcgggcct ggtgaactgg cgcatgatgg
     4381 ggcaggcact cctgctgtcg ggacggacgg actggggtat cgaattcttt ttcagggacg
     4441 ggggatcgga gcggttcctg tgctcgaaca ttccgggggc cggaaggctt ttcgacgccc
     4501 tccgccttca caaaattccc ctgtcggaag agcttcagcg ggctgcggaa ggaacggtcg
     4561 gccccggagc ggaagaggag agctgagtat tttttgtaaa ggcggtgaaa aaatgagaac
     4621 tgtactgcgc cgtattttta agggaccatc tgcccgcagg acctgccgtt tccttggcct
     4681 ggcaggcctg ctggtgtgcc aggccgccgt gaagccggga ggagcggaac cctcggtacc
     4741 cccttcgaac gaatggattc ataaccggat cgccgtactg gaacaggccc tttccctccg
     4801 gaacgaggcc catcgcatgg aggcggatgg caatgttccg ggagccatgg aggcctgctg
     4861 gaaaagtttc gtcctctatc ccgactcccg gctggagaaa aaggtttttt ccttccgccc
     4921 cgacctccag cccctgaagg accggatagc ctatctcgaa agccttcgcg cggccaggca
     4981 ggccgggccg gcgacagcct ccgggcctgc ggcagcgccc caggcgcccg gcggggattc
     5041 cgccgggcgg gacaatcccg actggagaac ctaccctgat cttccggcag aaaagaaggc
     5101 catcggtgag gttttttcct ctttccgaag cgcactgaag gcgggagaca cgaagagggc
     5161 ggcggcgtgc gtggatgaga gccgccggga gacctacgca gccctctttg aaaacaggcc
     5221 cgaggccatg ccgtccttcg ccgcccttct cgatgccgcg gaaatgagtt tcctgagtgc
     5281 gccggagagc gccgaccccg ggaccaccag cacgctgagg acctccgagt acgccgtgga
     5341 cgtgggggga ttcaccttct acgtgcgctg ggtcaaggtg gacggcaaat ggatgctctt
     5401 cgatttctga ccgcccggaa aggagggatg gagcgatgac gatgcatgat cgtattctga
     5461 aaaaagggac cgcaattctc gcgggacttc tgctgttcgc ggcggtctct cccgcccacg
     5521 gctggagcca gcatccccac cgccagatca atctggaggc ggtgaagctg ttcctgtccc
     5581 aggcgggagg caaggaaaaa ttctccatgg gccccttctc gccgaaggga ctttcggagc
     5641 cgctccgtgg tgttgctgtt accagctctt ccctgctcgc ctcggggttt gtctccgccg
     5701 aggcggcttt ctccgcccag tcgtggatca taaatggggg ggactgggct gacgagcccc
     5761 atctctattc ttccgtgcgg catttctacg accccctcag cctgtccggt tcggcctggc
     5821 tgaccgacca gagcg
//
LOCUS       DAOTHD010000015         5745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_16599_length_5745_cov_2.770475, whole genome shotgun sequence.
ACCESSION   DAOTHD010000015 DAOTHD010000000
VERSION     DAOTHD010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16599_length_5745_cov_2.770475"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="P5219_00545"
     CDS             complement(<1..428)
                     /locus_tag="P5219_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRX25273.1"
                     /translation="MEKQFSVEVGGRPLVFATGKVAKQANGAVVASHGETTILATACI
                     TDKPRTGIDFFPLLVDFEERFYSAGKIPGGFIKREGRPSETAVLSCRMVDRSIRSLFD
                     ETMRHDVHVVATVLAVVQVNPPNVLAINAASAALSISDIP"
     gene            complement(559..825)
                     /gene="rpsO"
                     /locus_tag="P5219_00550"
     CDS             complement(559..825)
                     /gene="rpsO"
                     /locus_tag="P5219_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRX25274.1"
                     /translation="MLEKDKKQGIISEYRTHGADTGSPEVQVAMLTQRIRDLTEHLKI
                     HTKDFHSRRGLLKMVGTRRKLLRYLRDKDFNRYKTLIERLGLRH"
     gene            1119..1799
                     /locus_tag="P5219_00555"
     CDS             1119..1799
                     /locus_tag="P5219_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRX25275.1"
                     /translation="MPLLIIEDNKDLVQVLSEGFAENGFSVDSAFSGEEGLEKISRGS
                     YGCIILDIMLPGIDGLQVLDTVRAQGNETPVLLLTARDTVEDRVDGLNRGADDYLSKP
                     FDFRELVARINALLRRTADRHHSVLRCGSLELDPVSRECRIGGKPLSLRRREFDILEL
                     LLRNENQVFTRENLISSVWRKEYSGSSNVVDVHVKYLRDKLRPFGCDTLVVTVRGVGY
                     KMNCPEYS"
     gene            complement(1859..3286)
                     /locus_tag="P5219_00560"
     CDS             complement(1859..3286)
                     /locus_tag="P5219_00560"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HRX25276.1"
                     /translation="MKPANRALPFFGTSKALLAALLVFAALFASVPAFAQDPFSGNPV
                     AKIARELSPAVVNIDVEAMVTRPVHPFADDPFFKQFFGEEFRRFSRTVPMKGRGSGFI
                     VSKDGHVLTNNHVVDGADKITVTFSDGKVHEAKVLGKDPTFDLAVLKIDGNGKDFPVL
                     ELGDSDKSEVGEWVVAIGNPFGLEHTVTVGVISAKNRSIHAGNVNFDGFLQTDAAINP
                     GNSGGPLIDLSGRVVGINTAIVPYAQGIGFAVPVNMAKQVMDDLIRYGSVKRGWLGVY
                     IQPLTRDFAEAYGIEEENGAVISDVLADSPAEKAGLQRGDVILTVNGEKIKDHQEFVS
                     RIRQRLSGEKVTLGIIRQKKKSDVPVTLGEMPGNEGTAIAASSDGAKMLKDLGLEVAP
                     VSEEIQKKHNLKNSEGLAVTAVEEGSYAARGGIREGDILLEVNGIRLKKAGDLEKVAS
                     RRGRSTVLLVWRDGRTFFVSIRGRN"
     gene            complement(3368..4147)
                     /locus_tag="P5219_00565"
     CDS             complement(3368..4147)
                     /locus_tag="P5219_00565"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRX25277.1"
                     /translation="MRPVFLELAGIQVHSYYVLWTFALCLAVILTRRRMTGLYSVPDD
                     NARVIIALSFTGMLIGARAGSAVEFWEMYSAEPWRLLRFWEGGLSAVPAFLGAGVAGV
                     AASWKLGVPVWATADSAAVPAAFTVALGRWGCFLNGCCGGTETSVPWAVTFPADPAGV
                     LRHPVQLYYAFGALFIGALLQWTEHNLLSFGEDRRIRGAVLWPLFMVSYALLRITADP
                     FRAEYGTAGLRSARIIAFAVLASGVLWLACSLLYRRERKKC"
     gene            complement(4150..4815)
                     /gene="jag"
                     /locus_tag="P5219_00570"
     CDS             complement(4150..4815)
                     /gene="jag"
                     /locus_tag="P5219_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HRX25278.1"
                     /translation="MNDNEVLVLDVPSVEEAQKTAAGQWGLDPSDVVVKVIEEEKSFF
                     GLLGRKLRVEVRPVAPLSALRGKALVARLLDMMDLHITLELADDNRINLTGQDAGIII
                     GKYGETLKSMEFLLNLMTRGVDEGERIFLDSDGYRERRELSLQRLALAAARKSVKRGK
                     PTYLEPMTSWERRVIHITLKDDSTVETKSVGEAPTRKVVVWPVTPARSSSGPARRPGR
                     RRA"
     gene            complement(4856..5644)
                     /locus_tag="P5219_00575"
     CDS             complement(4856..5644)
                     /locus_tag="P5219_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HRX25279.1"
                     /translation="MGSIWKMGGDLMMKLMELVHGFTGSWGLAIITLTLIVRLLLHPL
                     TQKQMASMQKMQKLQPRIKMLQEKYKDDKESLNREMMALYRENKVNPAAGCLPLLVQL
                     PVFILLYQVLTNYDFSGVSFLGIQLDGSVLTTLGRAIGLTVEKEQLGIMAVVYGIMAN
                     PAGLLNIGVYLANTLLLAGIGFLTWYQQQLTASGNPQMAMMNWFMPLFLTFICLSLPG
                     GVLLYWGVSSLLGVVQQLRMSRKTAAEMQQKPALFKDKPTKSGD"
BASE COUNT         1236 a         1650 c         1582 g         1277 t
ORIGIN      
        1 cagggaatgt cggaaatgga gagagccgcc gatgcggcgt ttatggcaag cacgttgggc
       61 gggttgacct ggacgaccgc cagcacagtg gccaccacgt gaacgtcgtg gcgcatggtt
      121 tcgtcaaaaa gcgagcggat ggagcggtca accatccggc agctcaaaac ggcggtttcg
      181 gaagggcgcc cctcacgctt gatgaagccg ccgggaattt ttcccgccga gtagaatctc
      241 tcctcgaaat caacaagaag ggggaagaaa tcgatccccg tcctcggctt gtcggtaata
      301 cacgccgtgg caagaatcgt ggtctcgccg tgactcgcca ccacggcccc atttgcctgc
      361 ttggccacct tgccggtggc gaatacgaga ggacggcctc ccacttcaac tgaaaactgc
      421 ttttccattc aatgttcctc ctctatatgt cttttctctt tttgtatgcg gagtaagaat
      481 accataatcc gggccgggaa aaccaaaaaa aatgcgggga caaacgtccc cgcaccgaat
      541 tttctaccgg ccggccgatc agtgccgaag cccgagccgc tcgatgagcg tcttgtaccg
      601 gttgaaatcc ttgtccctga gataccggag aagctttctt ctcgttccga ccatcttcag
      661 aaggccgcgg cgggaatgaa agtccttcgt gtggatcttc aggtgttccg tgaggtcacg
      721 gatgcgctgc gtgagcatgg caacctggac ctcgggagac cccgtatccg caccgtgggt
      781 acggtactcc gaaataatac cctgtttctt gtctttctcc agcatattct tcacctcaac
      841 aaaaaaatcc cgaaacccag aaaggagtct ttcatccgta tccgagtttg ggtcggaaga
      901 atagtaccat tttcaccgtc agaaagcaaa tctgcgggtg cttcccgtgt gtgcttccct
      961 caatcaccgc aggcagcagg gggcgggaga cacatttttc agcaaaattt cagattccgg
     1021 gccaccattt cattttgtcc tcgactttta aatatctctg tgctttaatt tctagagtta
     1081 attaaccagt tcccgtcacc tgaaagggag gaatttccgt gccgcttctc atcatcgaag
     1141 acaacaagga tctcgtccag gttctttcgg aagggttcgc tgaaaatggg ttttccgttg
     1201 acagcgcctt ttccggagaa gaaggcctgg agaagatctc caggggcagc tacggctgca
     1261 tcatcctgga tatcatgctc cccggaatcg acggacttca ggtgcttgac accgtcaggg
     1321 cccagggaaa cgaaaccccc gtccttctcc tcactgcccg ggatactgtg gaagacaggg
     1381 tagacggact gaacaggggt gccgacgatt atctttccaa acccttcgat tttcgggaac
     1441 tcgttgcgag aatcaacgct cttctccggc gcaccgcgga caggcaccat tccgtcctcc
     1501 gctgcggcag cctggaactt gatcccgtgt caagggaatg ccggatcggg ggaaaacccc
     1561 tttccctgag acggcgggag ttcgacatac ttgaactgct tctaagaaac gaaaaccagg
     1621 tctttacccg ggaaaatctc atctcctccg tgtggcgcaa ggaatacagc ggatcaagca
     1681 acgtggtgga tgtccacgtg aaatacctcc gggacaagct gcgccccttc ggatgcgaca
     1741 ccctcgtggt tacggtgcgg ggagtgggat acaagatgaa ctgtccggaa tacagctgac
     1801 gttaaggcaa gaaaaaaacc cgcccctgca ggggcgggtt tttctgtcgg gaatcctgtc
     1861 agtttcttcc ccgtatggag acgaagaagg ttcttccgtc cctccacaca agaagcaccg
     1921 ttgacctgcc tctccgggat gccacctttt caaggtctcc ggcttttttg aggcggatgc
     1981 cgttcacttc gagaaggata tctccttcac ggattcctcc gcgggcggcg taggacccct
     2041 cctccactgc cgtgaccgca agaccttcac tgttcttcag attgtgcttt ttctgaattt
     2101 cctctgaaac cggagccact tccagaccaa ggtccttgag cattttcgct ccgtcggaag
     2161 atgccgcgat ggcagttcct tcattccccg gcatctcgcc gagggtgact gggacatcgc
     2221 tcttcttttt ctgcctgatg atgcccaggg tcactttttc tcctgaaagg cgctgccgga
     2281 tcctggaaac aaactcctgg tggtccttga tcttctcgcc gttcacggtg agaatcacgt
     2341 caccccgctg aagccctgcc ttttcagccg gagagtcggc caggacatcg cttatgacgg
     2401 ctccgttttc ttcctctatt ccataggctt cagcaaaatc cctggtaagg ggctggatgt
     2461 atacgccaag ccagccccgc ttgacgctgc cgtagcgtat aaggtcatcc ataacctgct
     2521 ttgccatgtt cacgggaacg gcgaatccaa tgccctgggc gtatggaacg atggcggtgt
     2581 ttatgccgac cacacgtccg ctgagatcga tgagggggcc tccgctgttg ccggggttga
     2641 tggccgcatc ggtctggagg aaaccgtcga aattgacatt tcccgcgtgg atgctgcggt
     2701 tcttggcgga gatgacgccc acggtgacgg tgtgctcgag gccgaagggg ttgccgatgg
     2761 cgacgaccca ttcacctact tcgcttttgt ccgaatcacc gagttcaagt acgggaaaat
     2821 ccttcccatt tccgtcgatt ttcaggacgg ccaggtcgaa ggtgggatcc tttccgagaa
     2881 ccttcgcttc gtgcactttt ccgtcggaga aggtgactgt tatcttgtca gctccgtcca
     2941 ccacgtggtt gttggtcaga acatggccgt ccttcgagac gatgaagccc gatccccgcc
     3001 ccttcatggg aacggtccgg gaaaaccgcc tgaactcctc accgaagaac tgtttgaaaa
     3061 aagggtcgtc ggcaaaggga tggaccggtc tggtgaccat ggcctccacg tcgatgttca
     3121 ccaccgcagg ggaaagctcc cgtgcgattt tcgcaacggg gttgccggaa aaggggtcct
     3181 gggcaaaggc gggcaccgaa gcgaaaaggg ccgcaaagac caggagagct gctaggagcg
     3241 ctttggatgt tccgaaaaac ggtaatgctc tgtttgctgg tttcatggtg attcaccccc
     3301 aaaaacgttt tctcctattg tattccgaag gatcggcaat acatcgtttt ttttacgtat
     3361 ccccgtttca gcattttttt cgctccctcc tgtaaaggag agagcaagcg agccacagaa
     3421 ctcctgaggc gagtacggca aaggcgatta ttctcgcgct cctgaggcct gctgttccgt
     3481 attccgccct gaatggatcg gcggtgatcc tgagaagggc ataggatacc atgaaaagag
     3541 gccagagaac cgctcccctg atgcgcctgt cctcgccgaa actgaggagg ttgtgttccg
     3601 tccactgcag cagggcgcct atgaagaggg ccccgaaggc gtaatacagc tggaccggat
     3661 gacggagcac cccggcagga tctgccggaa atgttacagc ccagggaact gatgtttccg
     3721 ttcctccgca gcatccgttc aggaaacacc cccagcgtcc cagggctacc gtaaacgccg
     3781 cgggtactgc tgcgctgtca gcggttgccc acaccgggac ccccagcttc catgacgcgg
     3841 ccactccggc aacaccggcg cccagaaagg cgggaacggc ggaaagtcca ccttcccaga
     3901 aacggagaag tctccagggt tcggcggagt acatttccca aaactccacg gcagaccctg
     3961 ccctggcgcc gataagcatc ccggtgaagg aaagggctat tatcaccctg gcgttgtcgt
     4021 caggcacgga gtagagcccc gtcatccgtc ttctggtcag gatgaccgca aggcacaaag
     4081 cgaaggtcca cagcacgtaa tagctgtgga cctggattcc ggctaattcc agaaagacgg
     4141 gacgcacctt taggcacgcc tcctgcccgg acgcctcgcg ggacccgatg aagagcgggc
     4201 aggcgtaacg ggccagacga caactttcct ggtgggtgcc tcgcccacgg acttcgtttc
     4261 cacggtgctg tcgtccttga gggttatatg gatcaccctc cgctcccagc tggtcatggg
     4321 ctcgagataa gtgggcttgc cccgcttcac cgacttccgc gccgccgcaa gggcgaggcg
     4381 ctggaggctc agttcccggc gttcccggta gccgtcgctg tcaaggaaga tccgctctcc
     4441 ctcgtcaact cccctcgtca tgaggttcag caaaaattcc atggatttga gcgtctctcc
     4501 gtattttccg atgatgatcc ccgcatcctg gcctgtaagg ttgatcctgt tgtcatcagc
     4561 cagttccagg gttatgtgaa gatccatcat atccaggaga cgggccacca acgcttttcc
     4621 cctgagtgcc gaaagagggg ccacagggcg gacttccacc cggagttttc ttccgaggag
     4681 accgaagaaa ctcttttcct cctctatcac cttgacgacc acatctgagg gatcgagccc
     4741 ccactggccg gctgccgttt tctgagcttc ttccacggag ggaacgtcga ggacgagaac
     4801 ttcgttatcg ttcacagcaa tcctcctttc aggagaggaa gcggctgatc agtcttcagt
     4861 cgccgctttt cgttggcttg tccttgaaaa gggccggctt ctgctgcatt tcagccgccg
     4921 tttttcgtga catgcgaagc tgctggacta ctccgagcag ggatgaaaca ccccagtaga
     4981 gaagaactcc tccaggcagg ctgaggcata tgaacgtgag gaaaagaggc atgaaccagt
     5041 tcatcatggc catctgcgga ttgccgctgg cggtaagctg ctgctggtac catgtcagga
     5101 acccgatgcc ggcgagcaga agggtattgg caagatagac gccgatattc aaaaggcctg
     5161 cgggattcgc catgatgccg tagaccacag ccatgatacc cagctgttcc ttttccactg
     5221 tcaggccgat ggcccggccg agggtggtga gcactgaacc gtccagctgg attccaagga
     5281 aggagacgcc ggagaagtca taattcgtca gcacctggta gagaagaatg aacacgggaa
     5341 gctgaacaag gaggggaagg cagccggccg cagggttgac cttgttttcc ctgtagagag
     5401 ccatcatttc cctgttgagg gattccttgt cgtccttgta cttttcctgg agcattttga
     5461 tgcgcggctg gagcttctgc atcttctgca tgcttgccat ctgcttttgc gtcagggggt
     5521 gcagaagaag cctgacgatc agcgtcagcg taataattgc aagaccccag gatccggtaa
     5581 agccatggac aagctccatg agcttcatca tcaggtctcc acccattttc cagattgaac
     5641 ccactatcaa tcaccgtcct cggagatata agccccgcct ttttcatgcc ggggcggcac
     5701 ggggtcgtat ccgccagaag cccacggatg gcaacgggcc agtcg
//
LOCUS       DAOTHD010000016         5734 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_16645_length_5734_cov_2.795739, whole genome shotgun sequence.
ACCESSION   DAOTHD010000016 DAOTHD010000000
VERSION     DAOTHD010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16645_length_5734_cov_2.795739"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="P5219_00580"
     CDS             complement(<1..214)
                     /locus_tag="P5219_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRX25280.1"
                     /translation="MEEQVMLERLREMMKESGAHLEGHFLLTSGNHSSDYMQCALLLR
                     FPRYAAFAGEALAKKVRKYSPDIIASP"
     gene            complement(199..915)
                     /gene="pyrF"
                     /locus_tag="P5219_00585"
     CDS             complement(199..915)
                     /gene="pyrF"
                     /locus_tag="P5219_00585"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRX25281.1"
                     /translation="MGTGLNHLILALDVERVEEGRDLLSALRGELKYVKIGHQLYARG
                     GIPFLKEIMAMDYAVFLDIKVHDIPNTVFMAVDALASEGLWALTLHCGGGRKMLEEAR
                     RAKDKAGSGTNLLGVTVLTSFDDASWEEVAPGCSMALALEKRASLCRETGMDGIVCSP
                     LDLPVVTAAGKGLFTVVPGVRPASVNDDQARTATPQKTIGGGADYIVVGRPIVNAPDR
                     IAAVRNIAAAVEEGLSWKNR"
     gene            complement(900..1859)
                     /locus_tag="P5219_00590"
     CDS             complement(900..1859)
                     /locus_tag="P5219_00590"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HRX25282.1"
                     /translation="MNSLRADLSIAVGGLNLETPVVIASGVWPYDPVLWSPERLEGVG
                     ALCTKAVSLQPREGNRGVRVWETPCGMLNSIGLQNSGVRGFLKEYLPIAGKSGIPFLV
                     NLVMESEREVRENLSILRESGDAIPAVELNISCPNADCDGMAWGISPESAARAAAIAR
                     SEWKGPLWVKLTPQAPDLPAVAKAVESEGADALVCGNTWLGMAMDLKRKQPVFRRVTA
                     GLSGPAVFPLALRTVWQVCGAVSIPVVGCGGVSRWEDAAAMILAGASAVEVGSSLFAD
                     FDLPRKISEGLADYLADAEIPNLAGLVGLGRKEKEGGRPWAPD"
     gene            complement(1840..2646)
                     /locus_tag="P5219_00595"
     CDS             complement(1840..2646)
                     /locus_tag="P5219_00595"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HRX25283.1"
                     /translation="MSCGGGSPRDYAAAVLGHRPLGDGSGLLAVRCGEIASCAEPGQF
                     VLLRPGTGNDPFLGRPLAVADAAGDVFKVVYRIVGRGTALLASKRQGDPVTVRGPIGR
                     GFFSARGEKPLPRKVILAGGAVGAAPLLFAARRLGLSRIEKTVMGVAGKGWEGFAEWL
                     KEAFPGVDLFSGDGTLGTKGTVLAGLPGTLPADSELWACGPQAMLSAIAAHYPRDGGR
                     ILAALESRMACGMGGCLGCVIPTVSGNRRVCVDGPVFTAEEVMWNELSSC"
     gene            complement(2643..3947)
                     /locus_tag="P5219_00600"
     CDS             complement(2643..3947)
                     /locus_tag="P5219_00600"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRX25284.1"
                     /translation="MILLKNATIFDGENLGTASEDLLLKDGVVARRGTDLSAEGAEVF
                     DLGGKLVCPGFIDLHVHFRDPGYEWRENTESGARAAAGGGYTTVVTMPNTDPAVDNPV
                     TVEYLVARGKKAGASRVLPAGCVSKGRQGKQLAELALMAESGAVFFTDDGAPVSDAKL
                     LKTALLYSRDLDVRIMEHPEEPSLSAKSQVNEGAVSAASGMKGCPASAEVIDIQRGII
                     LSRETDSPIHFTHVSTELGIDAIRQAKKEGLPVTCDVTPHHLSLDETYVLVSRFSSAY
                     KVNPPLRARKDVKALWDAVADGTVDAIVTDHAPYHLDDKDVPFQEALSGIASLECSVA
                     VVLDTWAKTGRPVPLERLLPLFTSGPAALLSRPWRSLGSLKEGAPGDVTVLDLEAIQR
                     VDVNQWKSKARACPWDGEMLQGWPVMALVEGRVVMNRLKDPE"
     gene            complement(3948..4874)
                     /locus_tag="P5219_00605"
     CDS             complement(3948..4874)
                     /locus_tag="P5219_00605"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HRX25285.1"
                     /translation="MEWRHKNLITLEGWTREELGFFLDQTEYMEELLNRPIKKVPALR
                     GKIVMNCFFENSTRTRASFEIAGKVLSADVINWSSSGSSASKGETLRDTVWTLEAMAA
                     DAVVVRHGEVGVPDYLAKKLTKASVFNAGDGTNGHPTQALLDLYTARQKLGNLEGAKM
                     AIIGDVLHSRVARSDIQAFRKMGVHVTLSGPATLMPENTASLGVGYVSDAREAAEGAD
                     ILYFLRMQRERQEDGLIPSIDEYHRRWGATDSLVSLASPRAVVMHPGPMNRGMEIASS
                     VADGPRSVILDQVRSGVAVRMALLYLCLGGVR"
     gene            complement(4858..5388)
                     /gene="pyrR"
                     /locus_tag="P5219_00610"
     CDS             complement(4858..5388)
                     /gene="pyrR"
                     /locus_tag="P5219_00610"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HRX25286.1"
                     /translation="MERVLRRIAVEIVERNKGLERVILLGIQRRGVYLAARLREMLRE
                     AEKVKVPSGELDITLYRDDISVLSDQPVVHSTSVPGDITGKKVVLVDDVLFTGRTVRA
                     ALDAITDLGRPERVQLAVLVDRGHRELPIAADYVGKTVPTSRSENVEVRVKELDGEDR
                     VVICERTGGTESGVEA"
BASE COUNT         1011 a         1994 c         1689 g         1040 t
ORIGIN      
        1 ccggggaggc aatgatatcc ggcgagtatt tcctcacctt tttcgccagg gcttccccgg
       61 cgaaagcggc gtaccggggg aagcggagca gcagagcgca ctgcatgtag tcgctgctgt
      121 gatttccgga agtgagcaga aaatgcccct ccaggtgggc gccgctctcc ttcatcattt
      181 cccggagcct ttcaagcatc acctgttctt ccaagacaat ccctcctcca ccgcagcggc
      241 gatgttcctc accgccgcga tcctgtcggg agcgttcact atgggacgcc caaccacgat
      301 ataatcggct ccgccgccta tggtcttctg gggggttgcc gtccgggcct ggtcgtcgtt
      361 cacggacgcc gggcggacgc cgggcaccac ggtgaacagg cccttccccg cggcggtgac
      421 cacgggaagg tccagggggg agcagacgat gccgtccatg cccgtctccc tgcagaggga
      481 agcccgtttc tccagggcca gggccatgga gcaccccggc gcaacctctt cccaggaggc
      541 atcatcgaag ctggtgagca ccgtcacccc aaggaggttc gtgccggaac ccgccttgtc
      601 cttcgccctc cgggcctcct cgagcatctt ccgtccgccg ccgcagtgga gggtaagggc
      661 ccacagcccc tccgacgcca gggcgtccac cgccatgaac acggtgttgg gaatgtcgtg
      721 gaccttgatg tccagaaaaa cggcatagtc catggccata atctccttca ggaaggggat
      781 tcctccccgg gcatagagct ggtggccgat tttcacgtat ttcagttctc ccctgagggc
      841 ggagagcaga tccctgcctt cctccacccg ttccacgtcc agggcgagaa tgagatgatt
      901 cagtccggtg cccatggtct tcctccctct ttctctttcc gtccgaggcc cacgaggcct
      961 gcaaggttgg gaatttccgc gtcagccaga tagtccgcaa ggccttccga aatcttccgg
     1021 ggaaggtcga agtcggcgaa cagggagctt ccgacctcca ccgcggaggc cccggcaagg
     1081 atcatggccg cagcgtcctc ccaccgggac acgccgccgc accccaccac cgggattgac
     1141 acggccccgc agacctgcca cacggtccgg agagccaggg ggaacaccgc cggccccgaa
     1201 aggccggccg tcaccctgcg gaaaacgggc tgcttccttt tcaggtccat ggccatgccg
     1261 agccaggtat tgccgcacac aagagcatcc gcgccctccg actccacggc cttcgccacg
     1321 gcgggaagat cgggagcctg gggggtgagc ttgacccaca ggggcccctt ccactcgctc
     1381 ctcgcgatgg ccgccgcccg ggcggcgctc tccggggaaa tgccccaggc catgccgtca
     1441 cagtccgcgt tggggcagga gatgttcagc tctaccgcag gaattgcgtc tcccgactcc
     1501 ctgagaatcg aaaggttttc ccgaacttca cgctcggact ccatcaccag gttcacaaga
     1561 aaggggatgc cggatttgcc ggcaatgggg agatactcct tcagaaagcc gcggacgccg
     1621 gaattctgca gcccgatgct gttcagcatg ccgcagggcg tttcccagac ccgcactccc
     1681 cggttcccct cccggggctg aaggctcacg gccttggtgc agagggcgcc cactccctcc
     1741 agccgctccg gcgaccagag gaccgggtcg tagggccaca cccccgaggc gataaccacc
     1801 ggggtttcaa ggttcagccc acccacggcg attgaaagat cagcacgaag agagttcatt
     1861 ccacatcacc tcctcggccg taaagacggg cccgtccacg cacaccctcc ggtttcccga
     1921 aacggtgggg atgacgcacc ccaggcaccc gcccataccg caggccatcc tcgattccag
     1981 ggctgccagt atccggcccc cgtcccgggg atagtgcgcg gctatggcac tgagcatggc
     2041 ctgggggccg caggcccaga gttccgagtc tgcgggcagc gtgccgggaa ggccggccag
     2101 caccgttcct tttgtcccga gggtgccgtc gccggagaag agatccacgc ccggaaaggc
     2161 ctctttcagc cattcggcaa atccttccca ccctttcccc gccaccccca tgaccgtctt
     2221 ttcaatccgg gacaaaccca gcctgcgggc ggcaaagagc aggggggccg cccccactgc
     2281 ccccccggcg aggatcacct tccggggaag gggcttctct cccctggcgg aaaagaagcc
     2341 ccttcctatg gggccccgca ccgtgacggg gtccccctgc cgctttgaag ccaggagagc
     2401 ggttcctctc ccgactatcc tgtataccac cttgaacaca tcccctgcgg catcagccac
     2461 cgcgagcggg cgtcccagaa aggggtcgtt tcccgttccc ggccgcagca ggacgaactg
     2521 ccccggctcc gcgcacgacg ctatctcccc gcacctcacc gcgagcagcc cggaaccgtc
     2581 gccgagagga cgatgcccga ggaccgccgc cgcataatca cggggggaac cgccgccgca
     2641 gctcattccg gatccttcag cctgttcatg acgacacggc cctccaccag cgccatgacg
     2701 ggccatccct gcagcatctc gccgtcccag gggcaggcgc gggccttgct cttccactgg
     2761 ttcacgtcca ccctctgaat cgcttcgagg tcgagcaccg tgacgtcccc gggggctcct
     2821 tccttcagcg accccaggga acgccacgga cgggagagca gggccgccgg cccggaggtg
     2881 aaaagcggga ggaggcgttc cagggggaca ggcctgccgg tcttcgccca ggtgtccagg
     2941 accaccgcga cggagcactc cagggaggcg atgccgctca gggcctcctg gaatgggacg
     3001 tccttgtcgt ccaggtgata gggcgcatgg tcggtgacta tggcgtccac cgtgccgtcg
     3061 gcgacggcgt cccacagcgc cttgacgtcc ttcctcgccc tgaggggggg attcaccttg
     3121 taagccgagg agaaccggct gacaaggacg tacgtctcgt cgagggagag gtggtggggc
     3181 gtcacgtcac aggtaacggg aagcccttct ttctttgcct gccggatggc gtctatgccg
     3241 agctccgtgc tgacgtgggt gaaatggatc gggctgtccg tctcccggct caggattatc
     3301 ccccgctgga tgtcgataac ctcggctgag gcggggcatc ctttcatgcc gctggcggct
     3361 gagacagccc cttcgttcac ctgggatttc gcgctcaggg acggttcctc cgggtgctcc
     3421 attatcctca cgtccaggtc cctcgaatag agaagggccg tcttcagcag tttcgcgtcc
     3481 gacaccgggg cgccgtcatc ggtgaaaaaa accgcccccg actcggccat cagggcaagt
     3541 tccgcgagct gctttccctg gcgtcccttg ctgacgcacc ccgccggaag caccctcgaa
     3601 gccccggcct tttttcccct ggccacaagg tattcaacgg tgacgggatt gtccaccgca
     3661 ggatcggtat tgggcatggt caccaccgtg gtgtagccgc ccccggcggc cgcccgggct
     3721 ccgctttccg tattctcccg ccattcatag ccgggatccc ggaaatgaac gtgaagatca
     3781 atgaatccgg ggcataccag cttccccccg aggtcgaaca cctcggcccc ctccgcggag
     3841 aggtccgtgc ccctgcgggc gaccacgccg tccttcagga gaaggtcctc ggatgccgtc
     3901 ccgaggtttt cgccgtcgaa aattgtggca ttcttcagca gaatcatcta acgcacacct
     3961 ccaaggcaaa ggtacagcag ggccatccgg accgccacgc cgctccggac ctggtcaagg
     4021 atgacgctcc ggggaccgtc cgccacggat gacgcgatct ccattccccg gttcataggc
     4081 ccgggatgca tcaccacggc ccggggcgaa gcgagggaca cgaggctgtc ggtggcgccc
     4141 cagcggcgat ggtattcgtc aatggatgga atgaggccgt cctcctgccg ttcccgctgc
     4201 atccgcagga agtagagtat gtccgcccct tcggccgcct cgcgggcgtc ggaaacgtac
     4261 cccactccga gggacgccgt gttttccggc atgagggtcg ccgggccgct gagggtcaca
     4321 tggacgccca tcttcctgaa ggcctggata tccgaccggg ccaccctgct gtggagcacg
     4381 tccccgatga tggccatctt cgcgccttcg aggttcccca gtttctgcct cgcggtgtac
     4441 aggtcgagca gagcctgggt cgggtgcccg ttcgttccgt ccccggcgtt gaagacggac
     4501 gcttttgtga gcttcttcgc gagatagtca ggaaccccca cttcaccgtg tcgcaccacc
     4561 acagcgtcgg cggccatggc ctccagggtc cacactgtgt cccgcagggt ctcgcccttg
     4621 ctcgcactcg accctgagga cgaccagttt atgacgtcgg cggagagcac ctttccggct
     4681 atttcaaagg aagctctcgt ccttgttgaa ttctcgaaga aacagttcat gacgatcttc
     4741 ccgcgaaggg cgggaacctt ttttatcggc ctgttcagca gttcctccat gtactccgtc
     4801 tggtcgagaa aaaagccgag ttcctcccgt gtccacccct caagggtgat gagattctta
     4861 tgcctccact ccactttccg tccctcccgt acgttcgcag ataacgaccc tgtcctctcc
     4921 gtcgagctct ttcacccgta cttccacgtt ctccgaccgg gacgtgggga ccgtttttcc
     4981 cacgtaatcg gccgcgatgg gcagttcccg gtgtcccctg tccaccagca ccgccagctg
     5041 aacccgctcg ggacgtccga gatccgtgat ggcgtccagg gcggcccgaa ccgtccggcc
     5101 ggtgaagaga acgtcatcca cgagaaccac ctttttcccc gtgatgtctc cgggaacgga
     5161 cgtgctgtgc accacaggct ggtccgagag aacggagatg tcgtcccggt acagggtgat
     5221 gtccagctcc ccggagggga ccttcacctt ctccgcctcc ctcagcatct cccggagcct
     5281 ggcggcgagg tacactcccc ttctctggat gcccagcagg atgacccgct ccagtccctt
     5341 gttccgctcc acgatctcca cggcgatccg ccggaggacc cgctccatgt cctccttccc
     5401 caggaccgag gctttctccg cccatttttg ctcagtctgt gccgtcactt ccccatcccc
     5461 ttccatagag taaaaaaagt gcccttctcc cggcgggaga aaggcatgag agcttcctga
     5521 aacacgtgaa aaggagcggc cggaaatacc ggaattcctg tctgttcttc atggcgcccg
     5581 cctccttttc ctggtctggg aggaggatac cacgaaacca gaaataatgc aagcggttgc
     5641 aggaaacctg aatccatggg cagcggagag cgtcaccggg gcccgccggg agaatacggg
     5701 gggaagcgtc gtccaggaac caccaccggc ttca
//
LOCUS       DAOTHD010000017         5700 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_16774_length_5700_cov_3.364216, whole genome shotgun sequence.
ACCESSION   DAOTHD010000017 DAOTHD010000000
VERSION     DAOTHD010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16774_length_5700_cov_3.364216"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="P5219_00615"
     CDS             complement(<1..303)
                     /locus_tag="P5219_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     N-terminal domain-containing protein"
                     /protein_id="HRX25287.1"
                     /translation="MKRVAINGLGRIGRLVLRRCMEVRPEDVEIVALNKPSSPQEMAY
                     FIKYDSVHGKARFSVEAGENSLILDGKQIPLFGERDPSKLPWKELEVDIVLECTGHF"
     gene            complement(604..1611)
                     /gene="gap"
                     /locus_tag="P5219_00620"
     CDS             complement(604..1611)
                     /gene="gap"
                     /locus_tag="P5219_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRX25288.1"
                     /translation="MKKIAINGLGRIGRLVLRCYMEAKPEDFEIVALNDLTPPSEMAY
                     LIKYDSVHRRAGFSVEAGDGVLVLDGKSVPLFSEKDPSKLPWKELGVDIVLECTGFFT
                     KREKAAAHLAAGAKRVIISAPAEDSDLTIVLGVNEKLYDPSKHSVVSNASCTTNSLAP
                     VTKVLHEAFGIESLMGTTIHAYTSTQVLVDVPKGGGRKGRAAAVSLVPATTGAAKAMV
                     PLFPELKGRMDMISVRVPVADGSLTDIVVHLKKEVSVASVNAALKAAAEGSLKGIVEF
                     CEDEIVSADIIGNSHSGIVDAPSTKVIMGHIAKVMIWYDNEYGYSNRMLELAMYMASR
                     E"
     gene            complement(1679..2836)
                     /locus_tag="P5219_00625"
     CDS             complement(1679..2836)
                     /locus_tag="P5219_00625"
                     /inference="COORDINATES: protein motif:HMM:NF019522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HRX25289.1"
                     /translation="MKKIVSYLALSALLLFLLVWGVVAGTDWYVLSRWDAKDSSVIVI
                     EDLLGKRADPASGEGGEWESEFVKLRILYRSGPRTGSREDVEIMQLADSRLTLLPGKE
                     YLLLADMFEDGTVQFSVSDRYRVPAVVGFITFACGILVIIAGRSGFKALVGLFLSLLF
                     LIGWFVPRIASGHPPVPFAILAVAAVSVATCFFVVRKRELRPIPVFGAVGGAAGASLT
                     GWVMVSLWQLTGLESDSAALLASTSPDLSLRGLLLAAVMVGSIGAVLDVAVSVTSSMG
                     ELYEYDPSIPRRRLWKAGISVGKDVLGSMINTLILAYLGSSLPFVVLIATEGADFIGL
                     LNDPHIAQEVLRSVAGTVGLLLTIPVTAAAGVWWISGRRRNSSVPDDGLAD"
     gene            complement(3027..4856)
                     /locus_tag="P5219_00630"
     CDS             complement(3027..4856)
                     /locus_tag="P5219_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HRX25290.1"
                     /translation="MEMKVALVHLASGTSEDLLLPGEVVRKYIGGSGLGTYLLFRYGS
                     PAADPLSPDNPLIFMNGPFQGTGIPTSGRHQVISKSPLTGAFGESDCGGTFGFHLKRA
                     GYDGLVFLGRSSSPVYAAVIDGRVEIRDGTSLWGKDTFETEEALFSAEGKPAGVACIG
                     PAGERMVLLANIMHDGRNARAAGRGGLGAVMGSKNLKAVVARGKEKTVMADEKKVREK
                     SAEKAKFYMEKLPAMTRFGTAGGVALAEQNGDLPLKNWSMGSWPEEVKSITGEAMADT
                     ILTGNWGCMACPIKCGREVAFDGISGAGPEYETIGMLGSNCLINDLKAIGRGNDLCNR
                     MGIDTISGGSAVAFSMELYERGIIDERTVGYPLQWGDGEAMVRLLTDISEKRGFGVVL
                     AEGTRKAAEKIGKGAEKYAIHSKGMDFPAHDPRCYKSLAAGYATSNRGACHLSGFSYS
                     FERSMTYPELGVDAVLDRTVDEGKGKVNVGFQNLMGVLDSLKMCKFPFAATRLEDLLV
                     WINGVTGWDMACGELMEAGERILNLKKLFNLACGLTGRDDALPERILREPRGSGGSAD
                     TLPDLESQLREYYVERGWTEEGIPLPEKIASLGLEEFLHRKDW"
     gene            complement(4895..5428)
                     /locus_tag="P5219_00635"
     CDS             complement(4895..5428)
                     /locus_tag="P5219_00635"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRX25291.1"
                     /translation="MSVLSVLFFTFIRIGLGAFGGGLATIPFIHHELVVSHAWLTERE
                     FAEVVSLAQMTPGPVAVNAATYVGYRLAGFAGSLTATIAVVAAPLALVSAAAWLLSRA
                     SGRTKAWAERVQRALRPVVAAMLFGAFWMVVRPLTKDWRLWLFTLVFFGISRFSPFRK
                     YPQLLLVAAGTAGTILL"
     gene            complement(5425..>5700)
                     /locus_tag="P5219_00640"
     CDS             complement(5425..>5700)
                     /locus_tag="P5219_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25292.1"
                     /translation="FLAILFLARILLLFLNAPSVRAFFLGATCAVAVIIGGVVYDMTK
                     ISFAGGWKDMAVFSLVSAVLLGFGLHPFIALGAGAALRLALGEGKKG"
BASE COUNT         1292 a         1674 c         1518 g         1216 t
ORIGIN      
        1 gaaatgtcct gtgcattcca ggactatgtc gacttccagt tctttccagg ggagtttgga
       61 ggggtctcgt tctccgaaga ggggaatttg ttttccgtcg agtatcaggg agttttcgcc
      121 tgcttctact gaaaaccggg cttttccgtg gactgaatcg tacttgatga aatatgccat
      181 ttcttgagga gacgatggtt tgttgagagc tactatctcc acatcttccg gccggacctc
      241 catgcagcga cgaagaacca gccggccgat cctcccgagt ccgttgatgg ccacccgttt
      301 cattcattcc acctccataa aggataacat catgcaagta tcttatcaca gaaagagagc
      361 atttatatgc tgttttctat tattttttca ttatgttgtt tttttatgac aaaaacaaag
      421 caaaagggag tttcaaagcg taacagcctg ataggtaaag aaaagagctg tctattcttc
      481 cttaaacagg attttcaaca ggtttgtgga aaatttcccg aaaaaaaacg gtgtttcccc
      541 gcacagagga aacaccgttt caagccgacc gtttctgttt tcgaaaaaag ctgttttaag
      601 agtttactcc cggcttgcca tgtacatggc aagctcgagc attctgttgg agtatccgta
      661 ttcgttgtcg taccagatca ttaccttcgc gatatgcccc atgatgacct ttgtggaggg
      721 ggcatccaca attcccgaat gggagttgcc gatgatgtcg gcggagacga tttcatcctc
      781 gcagaattcc acgatcccct tcaggcttcc ctcggccgcc gctttcaacg cggcgttaac
      841 cgacgctacg gagacctctt tcttcaggtg aaccacgata tcggtgaggg aaccgtcagc
      901 aaccgggaca cggacggaaa tcatgtccat cctgcccttc agctcaggaa agagtggaac
      961 catggccttg gcggccccgg tagtggccgg aacaagagag acggccgcgg cccggccttt
     1021 ccttccgcct ccctttggaa cgtccacgag cacctgggtt gacgtgtagg cgtgaatggt
     1081 ggttcccatg agggattcga tgccgaaggc ttcgtgaagc actttcgtca ccggtgccag
     1141 ggagttcgtt gtgcaggagg cattcgaaac caccgaatgc ttcgagggat catagagctt
     1201 ttcgttgacg ccgagaacga tggtcaaatc cgaatcctcc gccggagcgc tgatgatgac
     1261 ccgtttcgct cctgcggcaa gatgggctgc cgccttttcc cgcttcgtaa agaaacctgt
     1321 gcattcgaga acgatatcca ctcccagttc tttccaggga agcttcgagg gatccttttc
     1381 gctgaaaaga ggaaccgatt ttccgtccag cacgagaact ccgtctcctg cttccactga
     1441 aaagccggcc ctgcggtgga ctgaatcata cttgatgagg taagccatct cagaaggggg
     1501 ggtgagatcg ttcagcgcga cgatttcaaa atcctccggt tttgcctcca tatagcagcg
     1561 aaggacgaga cgtcctattc taccaagtcc gttaatcgct atttttttca ttgcagcacc
     1621 tccgtaatgg attgatccta tttgaactta gagaaggatt ttatcacaaa gagaaaattc
     1681 agtcagcaag tccgtcatcc ggcacagaag aattccgccg acgcccggaa atccaccaga
     1741 cccctgctgc ggccgtgacg gggatggtca gaagcagccc cacggtccct gccacgctcc
     1801 tcaggacttc ctgggctatg tgagggtcgt tgaggagccc gatgaaatcg gctccctcgg
     1861 tggctataag caccacaaag ggcagggagc tcccgagata agcgagaata agcgtattga
     1921 tcatgctgcc cagcacgtct tttcccacgc ttatcccggc tttccacagc cttcttcggg
     1981 gaatggaggg atcgtactcg tacaattcac ccatggagga agtaaccgac actgccacgt
     2041 cgagcacggc gccgatggag cccaccatga ccgccgcaag gaggagcccc cggagggaga
     2101 ggtccggaga ggtggacgcg aggagggcgg cgctgtcgct ttcaagtcct gtgagctgcc
     2161 acagggagac catgacccat ccggtaaggg aagcaccggc ggcgccccca acggccccaa
     2221 aaacagggat ggggcgaagc tcccgtttcc tgacgacgaa aaagcaggtt gccacggaaa
     2281 cggcggccac agcaagaatc gcgaatggaa caggcggatg tcccgaggct atccggggca
     2341 caaaccaccc tatgaggaac aggagcgaca gaaaaagccc caccagggcc ttgaagcccg
     2401 accttcccgc aatgatgacg agaatgccgc aggcaaaggt tatgaagccc accacggcag
     2461 gaacacggta ccggtcgctt acggagaact gaaccgttcc gtcttcaaac atgtcggcaa
     2521 ggagaaggta ttctttgccg ggaaggagag tgagcctgct gtcggcaagc tgcataattt
     2581 ccacatcttc cctcgaaccg gtcctgggac cgctccggta caggatgcga agcttcacga
     2641 actcgctttc ccactcgcct ccttccccgg atgcaggatc ggcccgtttc cccagcaggt
     2701 cttctatcac tatcaccgag gaatctttcg cgtcccatcg cgagaggaca taccaatccg
     2761 ttccggcgac tactccccag acgagcagaa acagcagaag cgccgataag gcgaggtaag
     2821 aaactatttt tttcattctt tttttccctt tcttcaggtc cgttttcctc cgaagaggcc
     2881 tgctgtctgc attgatcccc atcccggaac ggcagagcag gaaacgttcc ccgtcgtccg
     2941 ggattgtggt ctctctccct tctccgcagg gggaataacc ttcccggacc tccgcacgac
     3001 ctgcatacat gaggaaacgg gactgattac cagtccttac ggtgcagaaa ttcctccagg
     3061 cccagggaag caattttttc cggaagagga attccttctt ccgtccaccc ccgttcgacg
     3121 taatactccc ggagctggct ttcaaggtcg gggagggtgt ccgccgatcc ccccgatccg
     3181 cggggctccc ggagaattct ttcggggagg gcgtcatccc tgccggtaag accgcaggcc
     3241 agattgaaca gcttcttgag gttcaaaatc ctctcgcccg cctccatgag ctctccgcag
     3301 gccatatccc accccgtgac gccgttgatc cagacaagga gatcttccag tctcgtggcg
     3361 gcgaagggga atttgcacat cttcagggaa tccagcactc ccatgaggtt ctggaacccc
     3421 acgttgactt ttccctttcc ctcgtccacc gtcctgtcga ggactgcatc aacaccaagt
     3481 tcggggtagg tcatgctccg ctcgaaggaa taggaaaatc ccgaaaggtg gcaggctccc
     3541 cggttcgacg tagcgtagcc cgccgcgaga ctcttgtagc accgggggtc atgggcgggg
     3601 aaatccatgc cctttgagtg gatggcgtat ttttccgccc ctttgccgat tttctcagca
     3661 gcttttcttg ttccttcggc aaggaccact ccgaatcccc ttttttccga tatgtcagtc
     3721 agaagtctca ccatggcctc tccgtcgccc cactgcagag ggtacccgac cgttctttcg
     3781 tcgatgatcc cccgctcgta cagctccatg gagaatgcga ccgccgaacc tccggaaata
     3841 gtgtcgattc ccatgcggtt gcagaggtcg ttgccccgcc cgattgcctt caggtcgttg
     3901 atgagacagt tgctcccaag cattccgatg gtttcgtatt ccggccccgc gccggaaatg
     3961 ccgtcaaagg ccacttcacg gccgcacttt atcggacagg ccatgcagcc ccagtttccc
     4021 gtgaggatag tatcggccat ggcctcgccc gttatgctct tcacttcctc cggccagctt
     4081 cccatgctcc agttcttcag aggaaggtcg ccgttctgtt ccgccagggc aacgcctccc
     4141 gccgttccga atcgggtcat ggcagggagt ttttccatgt aaaatttcgc tttctccgct
     4201 gatttttccc gaaccttttt ctcgtccgcc atgacggtct tttctttccc cctggccaca
     4261 acggccttga ggttcttgga tcccatgacg gcacccagtc ctcctctgcc ggcagccctg
     4321 gcgttccgcc cgtcgtgcat gatgttcgcc agcagcacca tccgctctcc cgccggaccg
     4381 atgcaggcca cgccggcggg ttttccctcg gcggaaaaaa gagcttcttc cgtctcgaag
     4441 gtatcctttc cccagaggga ggtcccgtcc cggatctcca ccctgccgtc gataaccgct
     4501 gcgtagaccg gagaggaaga tcttccgaga aacacaagac cgtcatatcc cgcccttttc
     4561 aggtggaagc cgaaggttcc tccgcagtcg gactctccga aggcacccgt caggggggat
     4621 ttgcttatca cctggtgccg ccccgaggtg ggaattcccg tcccctggaa ggggccgttc
     4681 atgaaaatga gggggttgtc gggcgaaagc ggatccgccg caggggagcc atagcgaaaa
     4741 agaaggtacg tgccgagacc ggatccgccg atgtatttcc ggaccacttc gcccggaaga
     4801 agaaggtctt cagaagttcc ggaggccagg tgcacaagag ctactttcat ttccatagtg
     4861 aaatcattcc tttcatgagc ctgaggttca ttttctagag aagaatcgtg cctgccgtcc
     4921 ctgcggcaac aagaagcagc tggggatatt ttctgaaggg agaaaaccgc gaaatcccga
     4981 aaaagacaag agtgaaaagc cacagtctcc agtctttcgt cagggggcgg accaccatcc
     5041 agaacgcgcc gaaaagcatg gcggccacca cgggacgaag ggcccgctgc actctttccg
     5101 cccacgcttt cgtccggcct gatgccctgg aaagaagcca cgctgcggcg gaaacaagag
     5161 caagaggtgc ggccaccacg gcaatcgtgg ccgtcagcga accggcgaag ccggcaagcc
     5221 tgtagcctac gtaggtcgca gcgttgaccg ctacaggtcc cggggtcatc tgtgcaagag
     5281 agaccacctc ggcaaactcc ctctcagtaa gccacgcatg ggatacgacg agttcgtggt
     5341 gaatgaaggg aatcgtcgcc agccccccgc cgaaggcgcc gaggccgatg cggatgaagg
     5401 tgaaaaagag aacggaaagg acactcatcc ctttttcccc tccccgaggg caagacgaag
     5461 agccgctccc gcaccgaggg caatgaaagg atggagtccg aaaccaagga gaacagccga
     5521 aacaagggaa aagacagcca tatccttcca tccgcccgca aaggaaattt tcgtcatgtc
     5581 atagaccact cccccgatga ttaccgccac tgcacaggtc gccccgagaa aaaaagcgcg
     5641 gacggacgga gcattcagaa agagaagaag aatccttgcc agaaaaagaa ttgcgagaaa
//
LOCUS       DAOTHD010000018         5536 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_17438_length_5536_cov_3.473089, whole genome shotgun sequence.
ACCESSION   DAOTHD010000018 DAOTHD010000000
VERSION     DAOTHD010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17438_length_5536_cov_3.473089"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="P5219_00645"
     CDS             complement(<1..667)
                     /locus_tag="P5219_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRX25293.1"
                     /translation="MAAVCDKKYDVIVVGGGHAGCEAALAAARMGAAVLQLNLYLDNT
                     ALMPCNPSIGGPAKGHLVREGSALGGEQAEAADSSAMLVRWLNTSKGPAVQALRAQCD
                     LRDYAAHYTLAVETQKNLDVHQDVVTDLWTEKGRIRGVRTKYGLVYEGKTVVLATGTY
                     LGGKVYIGEDSFASGPLGQVPSEELSRSIRGAGLETARTRTDTTPRLHMDSLDLSSLT
                     AQGS"
     gene            complement(703..1758)
                     /gene="recF"
                     /locus_tag="P5219_00650"
     CDS             complement(703..1758)
                     /gene="recF"
                     /locus_tag="P5219_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660078.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HRX25294.1"
                     /translation="MWCSEYLVRNFRNLSPVRISWDPGLNLVTGKNGAGKTNCLEGLH
                     LLTGWGPFGERKDIPSWRGEGDRAYITGSFCGEEDLFLAMAVGGSTVVKCDGKRVSFP
                     EVRARVPALAFLPGDMALLDGSPSIRRNFLDRLCALLFPLYARRISDFRRAVRHRTVL
                     LRAGKRTEPLSRAMAPLAAWIWSCRDEAVGLLSLGLARFQDLSPAPLELAHVRGGGSI
                     AADPLDDWWQSLEAWGERERRSCLSLVGPHRDDLLVRTGGRDAAVFFSRGQIRRASVA
                     LMLAAGKAVEARLKRKPLILLDEIASELDGEGRRITVDSLEGTGWQVVAAAAEMPEDQ
                     WPGSMWKVEEGAVLPCV"
     gene            complement(1762..2946)
                     /gene="dnaN"
                     /locus_tag="P5219_00655"
     CDS             complement(1762..2946)
                     /gene="dnaN"
                     /locus_tag="P5219_00655"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRX25295.1"
                     /translation="MRLQINKGEFMKNWQIAERSTSSKSTINSLTGILLKASGQDDEN
                     TVRLEATDLKTSVKCISRGILVEEEGEAVLPVKVVGELFKKAPTSVFTVSVTDGKGLI
                     IAGRNRYKFTTYPSREFPTLPVSESAPFFCSISASELLRTISEGTVASTVGEEFPKYL
                     GTAFFQMKDGELRVISTDGRRLSLSKCYPAEKGEDSDFLLPITGLKELQRLLSSVDGE
                     TPVQVLSDTTLVFFQMGSLEFSVRRVEAAFPNYEKILNPQSTTSLDVDRNSLASALER
                     VDVIVREFSRMVLFRLSPQGDLVLMGKAPETGAVEEILDGKIEGEPLTVAFNVGFLLD
                     GLKALYGDRAFISFNGPEGQMTMLRPDQKDFLYMVMPIKLTESDLSFEEDDGEEEDFP
                     TE"
     gene            complement(3172..4500)
                     /gene="dnaA"
                     /locus_tag="P5219_00660"
     CDS             complement(3172..4500)
                     /gene="dnaA"
                     /locus_tag="P5219_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRX25296.1"
                     /translation="MVEKDLNRLWEEILAEATRSLPAGTADLWLKTCIPTDLVEGSLV
                     LDVPNVFVKEQISSRFLKDLVRICRETGKSDDIELRVGSETKKDEQKRAQKAVSGGSA
                     ESRGGLNPGYLFNSFVVGKSNRLAHAASLAVAETPGEAYNPLFIWGGVGLGKTHLMHA
                     IGHYVLDRNPNHKVTYVSSEKFINEFIQSIKNNKTQEFKSKYRSVDILLIDDIQFLGN
                     KGSSQEEFFHTFNQLHTSKKQIVICSDRPPKDIQSIEDRLVSRFEWGLVTDIQSPDLE
                     TRIAILQKKAQLRRYDIPDDVINFLAVNIPSNIRELEGALNRVVACAELSSEPITEEN
                     TGEWLKDVIRKDIRGPVSVDAIQHAVAEFFSMSVDDLLSAKRTSDLALARQIAMFLCR
                     KLTEVSLQQIGLSFRKKDHTTVLHAHRKISQMVKEQPRIRQIVDTIEGKL"
     gene            4946..>5536
                     /locus_tag="P5219_00665"
     CDS             4946..>5536
                     /locus_tag="P5219_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HRX25297.1"
                     /translation="MSSSLLHVISHTDLDGIAAAAVAWHRWRGERPLKVSLAGYGAVD
                     GLILESIAAGQDFLVVDLFCQDSRTVDALDRHYSEGGDPFVFDHHETTAARYGARPWA
                     VVDTAFCAAKVYFRWLLGRGAPGMERLAPLVELANDRDLWINENPDSRLWQALITLCG
                     PYSLLARLAENPDPSLAPSERAAGEEFVEKQEKRFAL"
BASE COUNT         1102 a         1640 c         1580 g         1214 t
ORIGIN      
        1 cgctgccctg ggcggtaagg gaagaaaggt ccagggaatc catatgaagc cggggagtgg
       61 tatcggtacg ggtcctcgcc gtctcaaggc ccgctccccg gatggaccgg gagagttcct
      121 ccgagggaac ctgcccgaga ggaccggaag caaaactgtc ctccccgatg tagaccttgc
      181 ctccaagata ggttcccgtg gcgagcacta ccgtttttcc ctcgtagaca agaccgtact
      241 tcgtccggac acccctgatc ctgccctttt ccgtccagag atccgtcacc acgtcctggt
      301 ggacgtccag gtttttctgg gtctccacgg cgagggtgta atgggcggca tagtcccgga
      361 ggtcgcactg ggcacggagc gcctgtacgg caggtccctt tgacgtgttc agccagcgga
      421 ccagcatggc ggagctgtcg gcggcctctg cctgctctcc tccgagggcg cttccttccc
      481 tgacgaggtg cccctttgcc ggccccccta tggagggatt gcagggcatg agggccgtgt
      541 tgtccaggta aaggttgagc tggagcaccg ccgctcccat ccgtgccgcc gcgagggccg
      601 cctcgcaccc ggcgtgacct cctcccacaa caatgacatc atatttttta tcgcacactg
      661 ccgccattcc attctcttcc tttcaggatc ccgtttctgt tctcaaacgc agggaagcac
      721 cgctccctcc tccactttcc acatgctgcc cggccactgg tcctcaggca tctccgccgc
      781 agcagcaaca acctgccacc ccgttccctc cagggaatcc acggttatcc tccgtccctc
      841 cccgtcgagc tcagaggcaa tttcgtcaag aaggatcagg ggcttccgtt tcagccgggc
      901 ctccacggcc ttgcccgccg cgagcatcag ggcgacggag gccctccgta tctggcccct
      961 gctgaaaaaa acagccgcat cccgtcctcc cgtccgcacc aggagatcgt cccggtgagg
     1021 ccccacgagg gaaagacagc ttcttctttc cctctcgccc caggcctcga gggactgcca
     1081 ccagtcatca agaggatctg ccgctatcga accgcctccc cggacatggg ccagctccag
     1141 gggagcgggg gagaggtcct gaaaacgggc cagcccgagg gaaaggagac cgacagcctc
     1201 atccctgcac gaccatatcc acgctgccag aggagccatg gcacgggaca ggggctccgt
     1261 cctcttgccc gccctgagaa gaacggtcct gtggcggacc gccctcctga aatcgctgat
     1321 tctcctggcg tacagaggaa agagaagggc gcataatcta tccagaaaat ttcgacgaat
     1381 agaaggcgat ccatccagca gggccatatc ccccggaaga aacgcgagag cgggcaccct
     1441 cgcccgcacc tcgggaaagg aaacccgctt tccgtcgcac ttcaccacgg tagaaccgcc
     1501 gacggccatg gcgagaaaca ggtcctcctc gccgcagaag gaaccggtaa tatacgccct
     1561 gtctccttcc ccccgccagg aagggatatc ttttctttcg ccgaaaggac cccatcccgt
     1621 caggagatga agtccttcaa gacagttggt ttttcccgcc ccgttttttc ccgtgacgag
     1681 gttcagtccc gggtcccagg aaatccggac aggcgacagg tttcgaaagt ttcgtacgag
     1741 gtactcggaa caccacatgc cttattcggt gggaaaatcc tcctcttccc cgtcatcttc
     1801 ctcgaaggag aggtcgcttt ccgtgagctt gataggcatg accatgtaca gaaagtcctt
     1861 ctggtcagga cggagcatgg tcatctgtcc ctccgggccg ttgaagctga tgaacgcccg
     1921 gtcgccgtag agggccttca ggccgtcgag gaggaagccc acgttgaagg ccaccgtcag
     1981 gggctcacct tcgatctttc cgtcgaggat ctcctccaca gctcccgttt cgggagcctt
     2041 tcccataagg acaagatctc cctggggaga aaggcggaaa agcaccatgc ggctgaattc
     2101 ccggacgatg acatccaccc gttccagggc tgacgcgagg gaattcctgt ccacgtcaag
     2161 gctggtggtg ctctgggggt tcaggatctt ttcgtagttc gggaacgcgg cttccactct
     2221 ccggacggag aattcaagac tccccatctg gaagaacacc agggtggtat cggaaaggac
     2281 ctggacaggg gtttccccgt caacggagga aagcagccgc tgcagctcct tcaggccggt
     2341 gatgggaagg aggaagtcgc tgtcctctcc tttttccgcc gggtagcatt tggacaggga
     2401 aagccgccgc ccgtcggtgg aaatgacccg gagctctccg tccttcatct ggaagaacgc
     2461 cgtgccgagg tacttgggga attcctctcc cacggtgctt gccacggttc cctcggagat
     2521 ggtccggagc agctcggaag cgctgatgga gcagaaaaag ggagcgcttt ccgatacggg
     2581 aagggttgga aattcccggg aagggtaggt ggtgaacttg taccggttcc ttcccgcgat
     2641 aatcaggcct tttccgtcgg tgacggagac ggtgaaaacg ctggtgggag cttttttgaa
     2701 gagttctccc acgaccttga ccggcaggac cgcttctccc tcttcttcca cgaggattcc
     2761 ccgggagatg cacttgacgg atgttttcag gtcggtggcc tcgaggcgga cggtattttc
     2821 atcgtcctgg ccggaggctt tcagcagtat gcccgtaaga gagttgatgg tgcttttcga
     2881 actggtgctc ctttcggcga tctgccagtt tttcatgaac tctcccttgt tgatctgaag
     2941 tctcacaatg tttccctccc cgaagtacat cttttattat tttttggttt tagtgacagt
     3001 aatgataata ggcagtgcga atactgtgga taacccgacg gccgggccct atgacggagt
     3061 tttccactgt ggacagattt tcccctgacc ggcaaagtta ttcacagtat ccacaggaaa
     3121 atagaataat ctccgattga ggccggctta tccacaaaag gggaaatccg ttcacagttt
     3181 tccctcgatt gtatccacaa tctgccggat tcttggctgc tccttcacca tctggctgat
     3241 cttcctgtgt gcgtgaagca cagtagtatg gtccttcttc cggaaggaga gaccgatctg
     3301 ctgcaggctg acctcggtaa gcttgcggca caggaacatg gcgatctgcc gggcgagggc
     3361 caggtcggac gtccttttgg cgctcagaag atcgtccacg ctcatgctga agaattccgc
     3421 cacggcgtgc tggatggcgt ccacgcttac cggcccgcgg atatcctttc ggatcacgtc
     3481 cttgagccac tcaccggtat tttcctcggt gatgggctcg ctggaaagct cggcgcaggc
     3541 cactaccctg ttgagggccc cctcgagctc ccggatgttg ctcgggatgt tgacggcaag
     3601 aaaattgatc acgtcgtcgg ggatgtcata gcggcggagc tgggcctttt tctgcaggat
     3661 ggcgatcctg gtttcgagat ccggtgactg gatgtccgtg acaaggcccc attcgaaacg
     3721 gctcacgagc ctgtcctcga tgctctggat atccttcggc ggccggtcgg aacagatgac
     3781 gatctgtttt ttcgacgtgt ggagctggtt gaaggtgtgg aaaaactctt cctgactgct
     3841 ccccttgttt ccaaggaact ggatatcgtc gatgaggagg atgtccacgc tcctgtactt
     3901 ggacttgaac tcctgggtct tgttgttctt gatggactgg atgaactcgt tgatgaactt
     3961 ctccgaactc acgtaggtca ccttgtggtt cgggttcctg tcgaggacgt aatggccgat
     4021 ggcgtgcatg agatgggttt tccccaaccc gacgcctccc cagatgaaga gaggattgta
     4081 ggcctccccc ggcgtctccg ccacggcgag acttgccgcg tgggccagcc tgttggactt
     4141 tccaaccacg aaggagttga aaagatagcc cgggttgagt cctccgcggg attcagcgga
     4201 gcccccggat acagccttct gggcccgttt ctgttcgtcc tttttcgttt ccgaccccac
     4261 ccggagttcg atgtcatcgc tttttcccgt ctccctgcag atgcggacaa ggtccttcag
     4321 aaacctggag gagatctgct ccttcacgaa cacgttggga acgtcgagaa cgagggaccc
     4381 ctccacaagg tcggtgggga tgcaggtttt aagccacagg tcagcggttc cggcgggaag
     4441 ggaccgggtc gcttcagcca ggatttcttc ccacagccgg ttgagatctt tttccacaat
     4501 gcttcacctc atgctgagaa tggcaccata ttatcacaac gggcggaatg tggataactc
     4561 gaaaagaagg cagccgacgg aaaagaggcg tattttccat tgcaggcttt gtgtatgaac
     4621 aaagaatgga aaatatgttg tccacaatac ttgtccacat atccacaaag ggggtttatt
     4681 tttgtggata ggtaaaaata cggataccgt cggccggaac aaagttgtta caaaattctc
     4741 cgtcggtcac acaaattttg agccgcttac gggaacgggt cttgcgggtt atccacaact
     4801 tgcccacagt tgtgcacaag ttcatttttc attttccctc tccggaggaa aaaacggcat
     4861 gtggacaact ccggcccgat gatggaaaat gaactccgtc ttcggatgag aaactcattt
     4921 tgtttatttc ggaggagaaa gagacatgag ttcttccctt cttcatgtta taagccacac
     4981 ggacctcgac ggcatcgccg ccgcggcggt tgcctggcac cggtggcggg gagagcgtcc
     5041 cctgaaggtt tccctggccg ggtacggtgc ggtggacggg ctgatccttg aaagcatcgc
     5101 cgcaggacag gatttcctcg tggtggacct cttctgccag gacagccgga ccgtggatgc
     5161 tctcgaccgt cattattccg aggggggaga ccccttcgtc ttcgaccatc atgaaaccac
     5221 ggcggcccgg tacggagccc ggccctgggc ggtggtggac acggccttct gcgcggcgaa
     5281 ggtctatttc cgctggctcc tcggcagggg tgcccccgga atggagcggc tcgctcccct
     5341 ggtggagctt gccaacgacc gggatctctg gatcaacgaa aaccccgaca gccgcctctg
     5401 gcaggccctc attaccctct gcggccccta cagcctcctg gccaggctgg cggaaaatcc
     5461 tgatccgtcc ctcgctccct ccgaacgggc cgccggggaa gaattcgtgg agaagcagga
     5521 aaaacggttc gccctc
//
LOCUS       DAOTHD010000019         5467 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_17707_length_5467_cov_3.475795, whole genome shotgun sequence.
ACCESSION   DAOTHD010000019 DAOTHD010000000
VERSION     DAOTHD010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17707_length_5467_cov_3.475795"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..673
                     /gene="tmk"
                     /locus_tag="P5219_00670"
     CDS             17..673
                     /gene="tmk"
                     /locus_tag="P5219_00670"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRX25298.1"
                     /translation="MEKAAANRKMTKNNGVFLTLEGIDGCGKTTQARILVGNLVSRFG
                     KEAVFWTKEPGGWPGGEKIRELLLEHGMQHPLSELFLFLADRCEHIRREVAPALSSGR
                     IVVCERYIDSTLAYQSWGRGISSEKIEDLFRWCCFPVPDLTFWIDLPPGKAYHRVSRR
                     GSPDRMEEKGQSFLENVREGFLFLSRRDPERIVSLDGNNDIETIAREIERRVEVFLSP
                     "
     gene            670..1107
                     /locus_tag="P5219_00675"
     CDS             670..1107
                     /locus_tag="P5219_00675"
                     /inference="COORDINATES: protein motif:HMM:NF015822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF327 family protein"
                     /protein_id="HRX25299.1"
                     /translation="MKVKKNAEERISGGESGFQAKKKGLLSPSRGSEPHPFSQSLEDA
                     ETRELIEKLDLFGRKLTHFPAENTLLQYRNLVKELLRRAIAGLRVRRDMKWRRNDRNL
                     FVTVEKVESIMEELEGVFQREGERTRMFQLMEEVKGCLISLLL"
     gene            1083..1853
                     /locus_tag="P5219_00680"
     CDS             1083..1853
                     /locus_tag="P5219_00680"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25300.1"
                     /translation="MPHLPASLTASESWSRLCSLAALDHFPHCVILSAPEELHGFVAR
                     EIAEVVLCLNRTGGDDCNACRAWSGEDHPDLVRGGEPGKAPAIDACREVIRAMAYRPV
                     VSKKRCAVIFSAERMLLPAANSLLKLAEEPPEYGVLFFLVSEDDSMLPTLKSRAWSVS
                     LRGAGRKEKKASPSTPEEWSRWVEKNSGSEIDDLIPQLEPWIGFLTESGDHASAGKME
                     RLRILLETRRLSRTMALDLTVLALKEGIAFEHLFGDFW"
     gene            1915..3150
                     /gene="ricT"
                     /locus_tag="P5219_00685"
     CDS             1915..3150
                     /gene="ricT"
                     /locus_tag="P5219_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HRX25301.1"
                     /translation="MAETMRGTELSLLGGPLSPEQEERYRNSCSDDSSDSQVKGGEPA
                     LQEIAFVREATAEDLNDAEAEREEENAVLLKARELLRNHNLSMKLVDVEFLLDRKKLF
                     FYFTSEQRVDFRAFVRDLAKEFKTRIELRQIGVRDEAKAVKGLAPCGQRCCCSYWLHR
                     FTPICIKMVKEQNLALNPTKISGICGRLMCCMSFEHHLYGELWKGLPNPGSKLRTPGG
                     TYLTDGVDLSSQSVRIRTPEGKEILVKTEDFQRFKETVMEGSPWEENGLKESRSIRDD
                     SFRPGRKKTGEPREDTTGEKPAPSRQDRRKTEEKPVPDQAKEKEGTSQAVPAKKKRRR
                     KRKPSGGEKTETPIISSPEKTPPAAEKPVQQQGQESKPVDGAQKKKRPNNRRRRSPKG
                     NREAPQQGQNTNSSSSPEV"
     gene            3157..4098
                     /gene="ftsY"
                     /locus_tag="P5219_00690"
     CDS             3157..4098
                     /gene="ftsY"
                     /locus_tag="P5219_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRX25302.1"
                     /translation="MAFFERLKQGLKGVRERWSGGLSLLFSGGVVDEAFWENLEEILI
                     SGDVGVDLTLSLIDSLKRDAEEKRIRTPEELFRLFEEMISSRLLAVPFMGEPLPVTPP
                     SPSVILLVGVNGSGKTTTAGKLASRWTKEGKKVIFAAADTFRAAAIEQLKVWGDRSGV
                     RVIAQQHGSDAAAVAFDALQAAKASGADVLIVDTAGRLHSKHNLMEELGKIHRVLEKE
                     KGDSRMEVLLVLDSVMGQNGLAQAETFNKMLPLTGVVLTKYDNTAKGGIILAIAHSLK
                     IPVRYVGLGEGEDDLRAFDVPEFVKALLGDDGHGQGN"
     gene            4082..4855
                     /locus_tag="P5219_00695"
     CDS             4082..4855
                     /locus_tag="P5219_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25303.1"
                     /translation="MGRETKRPPRRRKVEKNEVTLSELLRDLYLHGVEYCLLYSPPEG
                     HPVFSKEQIVSVLEKGESERSVSELATLCSSFSPLKDLLEKNVLSKKDKLFVLKDADL
                     RILPVSTIAEMTGEEIGTGVPLWWDAPLPFVSWAGERFSANRKAAGLLDGIPLTRGEG
                     SEFLRELPGGKCFLFREIHPCIFLVDDVSEDMGNAKEMTWWAAVGKAFVSRLRGKGFS
                     VEKMTEDSLSAVDERDEILTCVWEDDIVGFLKVKDGKNF"
     gene            4852..5379
                     /locus_tag="P5219_00700"
     CDS             4852..5379
                     /locus_tag="P5219_00700"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HRX25304.1"
                     /translation="MTALFFYPVKLVTGVLYPDETRWRWAEEKLSTLWGTPEMVSSPY
                     PFTVTDYYTDISPVLYRRFLSFPGLRNGGSLPQWKRTACRLERESGEQRKVNIDPGYV
                     NGARLVLASTKDHAHRVYIGEGIHGEVTLRFRHKKWESFDYTFPDFAQGLYDEFLSAV
                     RKQWIQEMELRRNTQ"
BASE COUNT         1409 a         1364 c         1557 g         1137 t
ORIGIN      
        1 gacgtgaagc cgtccgatgg aaaaagcggc ggccaacagg aagatgacga agaataacgg
       61 agtgttcctc accctggaag gcatcgacgg atgcgggaaa acaacccagg cgaggattct
      121 ggtcgggaac ctggtttcac ggttcggaaa agaagcggtg ttctggacaa aagaaccggg
      181 agggtggccg gggggggaga aaataaggga actccttctg gaacatggaa tgcagcaccc
      241 cttgagcgaa ctttttcttt ttcttgccga ccgatgcgag catatcagga gagaagtcgc
      301 ccccgcactc tcgtcaggtc ggattgtggt ctgtgaacga tatattgatt ccacccttgc
      361 ataccagtca tgggggcggg ggatctcatc tgaaaagatc gaagatctgt tccggtggtg
      421 ctgttttcct gttcctgacc ttaccttctg gattgatttg ccccccggga aagcctatca
      481 tagagtctcc cgccggggaa gcccggaccg tatggaagag aaaggacaat cctttcttga
      541 aaatgtccgt gaaggctttc tttttctttc ccgccgggat ccggagcgga tagtttctct
      601 ggacggaaac aacgatatcg agactatcgc ccgggagatt gaacgcaggg tggaggtgtt
      661 tctttcccct tgaaggtcaa gaaaaatgcc gaagagcgga tttccggagg ggaatcggga
      721 ttccaggcaa agaaaaaagg actgctttcc ccttcccggg gaagtgaacc ccatcctttt
      781 tcccagtccc ttgaagatgc cgagacccgg gaactcatcg aaaaactgga tctcttcggc
      841 agaaagctga cgcactttcc tgcggaaaac acccttctgc agtacaggaa cctggtgaaa
      901 gaactcctgc gaagggcaat cgccggcctt cgtgtccgca gggacatgaa atggaggcgc
      961 aatgaccgga atctgttcgt aacggtcgag aaagttgaat ccatcatgga ggagcttgaa
     1021 ggcgtttttc agagggaagg ggaaagaacc cggatgttcc agcttatgga agaggtgaag
     1081 ggatgcctca tctccctgct tctctgacgg cttctgaatc ctggagtcgg ctctgttccc
     1141 tcgccgctct cgaccacttt ccccattgcg tgattctgtc cgcccccgag gaacttcacg
     1201 gattcgtcgc cagggaaatt gcggaggtcg ttctctgtct gaaccggacc ggaggcgatg
     1261 actgcaatgc gtgcagggct tggtccgggg aagaccaccc tgacctcgtc aggggcggtg
     1321 aaccagggaa agctcccgcc atcgacgcct gcagagaagt catacgggct atggcatacc
     1381 gcccggtggt ctcaaaaaaa cgatgcgccg tcattttttc ggccgaacgc atgctgttgc
     1441 ctgcagccaa cagccttttg aagctcgcgg aagaaccccc ggaatacgga gtcctgttct
     1501 ttctcgtctc agaggatgat tctatgctcc ccacattgaa aagcagggcc tggtccgtat
     1561 ctctcagggg agcgggaagg aaggaaaaaa aagcgtctcc gtccacaccg gaagaatgga
     1621 gcaggtgggt ggagaaaaac agcggttcag agatagatga tttgatcccc caactcgaac
     1681 catggatcgg ttttctcacg gaatccggcg accacgcaag cgcaggaaaa atggagcgcc
     1741 tgcggatttt gctggaaacc aggcgtcttt cccgaaccat ggctcttgat ttgacagtgt
     1801 tggctctcaa ggagggtatt gcttttgagc atttatttgg cgatttttgg taaaccaaga
     1861 tatcttggtc ttctcgatct tcaggaacag gtacctcagc gaggtgcgtg gcttgtggcg
     1921 gaaacgatga gggggacgga actctccctt ctgggcggac ctctttcccc cgaacaggag
     1981 gaacgttaca ggaattcctg cagtgacgat tcctcggaca gccaggtgaa agggggagag
     2041 cccgcacttc aggaaatagc ttttgtcagg gaagcgaccg cggaggattt aaacgacgca
     2101 gaagcagaac gagaggaaga aaacgcggtc ctcctgaaag ccagggagct tcttcggaac
     2161 cacaatctct ccatgaaact tgtggatgtc gagttcctcc tcgacagaaa aaaactcttc
     2221 ttctacttca catcggaaca gagagtcgac ttcagagcat tcgtccggga tctggcgaaa
     2281 gaatttaaaa ccaggataga actcaggcag atcggcgtgc gggatgaagc caaagctgta
     2341 aagggactgg ctccctgcgg gcagaggtgc tgctgcagct actggcttca ccggtttacc
     2401 cccatctgca tcaaaatggt gaaggaacag aacctcgcgc tgaatccaac aaagatatcg
     2461 ggaatctgcg gaaggctcat gtgctgcatg tccttcgaac accatttgta tggagaattg
     2521 tggaaaggac tgcccaaccc cggctccaag ctcaggacac cgggcggtac ctatcttacc
     2581 gacggggttg atctctcgag ccagtctgtc cggatcagaa ctccggaagg gaaggaaatc
     2641 cttgtcaaaa ccgaagattt tcagcggttt aaagaaactg tcatggaggg cagcccctgg
     2701 gaggaaaacg gcctgaagga aagcagaagc atacgggacg acagcttccg cccgggaagg
     2761 aaaaagacag gcgaaccccg ggaggataca acgggagaaa aacctgcacc ctccaggcag
     2821 gatcggcgaa aaacagagga gaaacccgtt cccgaccagg caaaagaaaa ggaaggcact
     2881 tcccaggcgg taccggcgaa gaaaaaaaga cgcagaaaga gaaaaccctc cggaggtgaa
     2941 aaaacggaga ctccgataat ctcttctccg gaaaaaactc cccctgcagc cgaaaaacca
     3001 gtacagcagc aggggcagga aagtaaaccc gtcgacgggg cccagaagaa aaagagaccg
     3061 aacaacaggc gccgccgctc tccaaaagga aacagggaag cgccccagca gggacagaat
     3121 accaacagca gctcttctcc ggaggtgtag aacgccgtgg ctttttttga aaggttgaag
     3181 caggggctga aaggcgtccg ggaacgatgg agcggaggac tgtccctcct cttttccgga
     3241 ggtgtggttg acgaggcttt ctgggaaaat cttgaagaaa tactcatcag cggcgatgtt
     3301 ggagtagacc tgacgctcag tcttattgat tcactgaaaa gagacgccga agaaaagagg
     3361 atccgcaccc cggaagagct tttcaggctg ttcgaggaaa tgatttcgtc ccgtctgctt
     3421 gctgttccgt tcatggggga acccctcccc gtcactcccc cttcaccctc cgtcatcctc
     3481 cttgtggggg tcaacggaag cggcaaaacc acaacggcgg gaaaactcgc ttcacggtgg
     3541 acgaaagaag ggaaaaaggt catctttgcc gcagcggaca ctttccgtgc cgctgccatc
     3601 gaacagctca aagtgtgggg agaccggtcc ggagtccggg ttatcgccca gcagcacggc
     3661 agcgacgcag ctgccgtcgc ctttgatgcc ttgcaggctg caaaggcatc cggggcggat
     3721 gtacttatcg tggatacggc cggaagactg cattccaagc acaacctcat ggaggagctc
     3781 ggaaaaatcc accgggttct tgagaaagaa aagggagatt cccggatgga agtgctgctt
     3841 gttctcgact ccgtcatggg gcagaacggt cttgcacagg cggaaacgtt caacaagatg
     3901 cttcccctga ccggcgtagt gctcaccaaa tacgacaaca cggccaaggg aggcatcatc
     3961 cttgccatag cccacagcct gaaaattccg gttcggtatg tcggcctcgg agaaggtgaa
     4021 gacgatctcc gggcatttga cgtaccagaa tttgtcaaag ctcttttggg agatgacggt
     4081 catgggcagg gaaactaaac gccccccccg gcggagaaaa gtggaaaaaa acgaagtgac
     4141 tctttcggag cttcttcgcg atctctacct ccatggagta gagtactgtc ttttgtattc
     4201 tcccccggag ggacatcccg tcttttcgaa agagcagatt gtttcggtgc tggaaaaggg
     4261 agaaagtgaa agaagcgtaa gtgaactggc gacgctttgc tcttcctttt cacctctgaa
     4321 agaccttctc gaaaagaacg tcctgagcaa aaaagacaaa ctttttgttc tgaaggacgc
     4381 cgacctgagg attcttcctg tttctaccat tgccgaaatg actggagaag aaatcggtac
     4441 aggtgttcct ctctggtggg acgccccgct tccattcgtc tcctgggcag gggaaaggtt
     4501 ctccgccaac cggaaagcag ccggacttct tgacggcatc ccccttaccc ggggtgaggg
     4561 gtctgaattt ctccgtgaac ttccgggagg gaaatgcttt ctgttcaggg agatacatcc
     4621 ctgcatattt cttgtggatg acgtttctga agatatggga aacgccaagg agatgacatg
     4681 gtgggcagcg gtaggaaaag ccttcgtctc acgcctcaga ggaaaaggtt tttccgtgga
     4741 aaaaatgacg gaagattctc tttccgccgt tgatgaaagg gatgaaatcc tgacctgtgt
     4801 ctgggaagat gatatcgtgg gatttctgaa ggtgaaggat ggaaaaaact tttgacggca
     4861 cttttttttt atcccgtaaa actcgtcacc ggggttcttt atcctgacga aacacggtgg
     4921 cggtgggccg aggaaaaact ttccacccta tggggaaccc cggaaatggt aagttccccg
     4981 tatcctttta cggtcacgga ttattacacg gatatttctc ctgtcctgta ccgccggttt
     5041 ctttcatttc cgggtcttcg gaacggcgga tctctgccgc aatggaaacg gaccgcctgc
     5101 cgactcgaaa gagaaagcgg ggaacagaga aaggtgaaca ttgaccccgg atacgtgaac
     5161 ggtgcacggc tggtacttgc atcgaccaag gaccacgccc acagggtcta tatcggagag
     5221 ggaattcacg gggaagtaac cctccgtttc aggcacaaaa aatgggagtc ttttgactac
     5281 acctttcccg attttgcaca aggtctgtac gacgagtttc tctcagccgt caggaaacaa
     5341 tggattcaag aaatggaatt gaggaggaac acacaatgat cgggaggtac caaactgcgg
     5401 aaatgaactc catttggagc gaagagagca agttcagcgc ctggctcgag gtggaacttg
     5461 cagtttg
//
LOCUS       DAOTHD010000020         5405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_17975_length_5405_cov_2.937757, whole genome shotgun sequence.
ACCESSION   DAOTHD010000020 DAOTHD010000000
VERSION     DAOTHD010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17975_length_5405_cov_2.937757"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="P5219_00705"
     CDS             complement(<1..263)
                     /locus_tag="P5219_00705"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRX25305.1"
                     /translation="MSLTQVFLLFLRLGAFTFGGGIVMMGLLERELRETDRFAHGEIM
                     DMMIFATAFPGPIAVNLALLAGRKLAGKAGAVVAVVGTVLPPFL"
     gene            complement(328..828)
                     /locus_tag="P5219_00710"
     CDS             complement(328..828)
                     /locus_tag="P5219_00710"
                     /inference="COORDINATES: protein motif:HMM:NF025946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRX25306.1"
                     /translation="MIDFSKGYSLEEYFRKGYEEERDRQKRACERTVFSPEFEEAVKR
                     VKQPVKLAAFAEIYCPDTVVAMPFVKRTADLNPLIRLAVFERDPWVKELEAFTGAARI
                     PTLLFFDEDMNLRGKYVELPDGLKDEMRDADQGERSTMTLEYRRGKYNGLIQEQFISI
                     LAAFSS"
     gene            942..1466
                     /locus_tag="P5219_00715"
     CDS             942..1466
                     /locus_tag="P5219_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25307.1"
                     /translation="MSPCSKCGADGPFLSLSTWSLCPVCREEALSDILESIRTLDCRF
                     ASLKSPPDGKAWADFTGNMIRLLDRLAAILPENLVPENEQPEDPGPFAMEEIRILLGS
                     SDLDEPRLRSAASMLDGILPFVENRSPENFAAFLRLRSKVFSALAKSDRDRAMSLLEK
                     KHPSPGDQSQLTLI"
     gene            complement(1485..2912)
                     /locus_tag="P5219_00720"
     CDS             complement(1485..2912)
                     /locus_tag="P5219_00720"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRX25308.1"
                     /translation="MVRSGIQSPDISKNAVLAVAALTAFLPPFMLSSVNIALPSIQQA
                     YGASAVWLSWVATSYLLSSAVFLVPAGKMGDIWGRKRILVLGTWIFILSTILCPLAPT
                     MPFFILLRSVQGAGGSLMLTMGMAILTSVFPAAERGKALGVNVASIYLGSSAGPFFGG
                     LLIAAFGWASVFFVNALLGAVSIILLKTRVPFEWREGSSKRLDLPGCFVYGASLVAVM
                     YGASILPSAGGVFLMAAGGGLFAVFIMRMLRSEDPLFEIRLFSGNRVFAFSNVAALIN
                     YAATYSVAFLLSLYLQYIKGMSPSEAGVVMVTQPLLQAFLSPYTGKLSDRIEPGLLAS
                     GGMLSTALGLTVFAFLGRETPMAFIFSALVLLGLGFAFFSSPNTNAIMGSVPSKFYGA
                     ASGSVGTMRVLGQMTSMAVITVVFAALLGSGQITPERYATFLVVVRISFSISSLLCFL
                     GVFFSWFRGNLHGGASGKGEREDVE"
     gene            complement(3077..3664)
                     /locus_tag="P5219_00725"
     CDS             complement(3077..3664)
                     /locus_tag="P5219_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HRX25309.1"
                     /translation="MNTKTTNVLLVGVGGQGTILASKVLTEGLLSFGYDVKMSEIHGM
                     AQRGGSVSTHVRYGEKVYSPVICRGEADVVVAFEKMEALRWLPYLSAGGRLIVNNHEI
                     FPMPVILGQAEYPREIEKNLSASAPGTLVVEAAKSADELGNPRVMNMVLLGAVVKTLG
                     LDGRDWGGTLRSCVPPGAVEINERAFAAGKTLLPG"
     gene            complement(3664..>5405)
                     /gene="iorA"
                     /locus_tag="P5219_00730"
     CDS             complement(3664..>5405)
                     /gene="iorA"
                     /locus_tag="P5219_00730"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HRX25310.1"
                     /translation="AVARGAWEAGCHVAAAYPGTPSTEILENLGRFPEVSSEWSPNEK
                     VALEVASGASIAGARALCAMKHVGLNVAADPFFTMSYIGVNGGLVVVTADDPGLSSSQ
                     NEQDNRWYALHAKVPMLEPSDSQECLDFTRAAFDISERFDTPVLLRLTTRICHSKSVV
                     ETEDRVESPVREYGRNPAKNAMLPAFARQKHYLVEKRLEDLREFGESSPFNSVTMADG
                     AEIGVIASGVGYQYAREALGDGANFLKIGFSWPLPRRRIREFASKVKTLWVVEENDPF
                     IETEIRAMGISCFGKDVLPVVDELTPAIVARAVLGKEPAPGREGEVPPPNRPPLLCPG
                     CPHRGLFFPLSKKKDVIVTGDIGCYGLGSMPPLNVGETTICMGAGFSAAIGFEKANEK
                     AGRNRKVFGILGDSTFFHSGITGLIDAVVNKSRSVFVIMDNRITAMTGQQENPGSGRT
                     LSGEETVALDIPKICGACGVKDDNIHVVDPYDLKASAEAVDRAWAAEETSVIVTTRPC
                     ALLKNVQKERAGMKCFVDEERCVLCGACLKLGCPAISKRDGRIVIDQEACNGCGLCVQ
                     VCPKSAVGKKGES"
BASE COUNT         1198 a         1670 c         1475 g         1062 t
ORIGIN      
        1 agaaagggcg gaagtactgt gcctaccacc gcaacaacgg ctccggcctt tcctgccagc
       61 tttctgcctg ccagaagagc cagattgaca gcaatgggac ccgggaaagc ggtggcaaaa
      121 atcatcatgt ccatgatctc gccgtgagcg aaacggtccg tttccctgag ctctctctcc
      181 agaagcccca tcatcacgat tcccccgccg aaagtgaagg ctcccaggcg gaggaagagc
      241 agaaaaactt gtgtgagcga catcagccca tcccggtctc cccggaacac tcccctcgaa
      301 tttccatgac ccggccggcc tgcggtctca tgaagaaaac gcggcgagga tggagatgaa
      361 ctgctcctgg atcaggccgt tgtattttcc cctgcggtac tccagggtca tggtactcct
      421 ttctccctgg tcggcatccc gcatctcgtc cttcaaaccg tcaggaagct ccacgtattt
      481 tccccggaga ttcatgtctt cgtcaaagaa gagcagggtg ggtatcctgg cggcgccagt
      541 gaaagcctca agttccttca cccagggatc ccgttcgaat accgccagcc ggatgagcgg
      601 gttgagatcc gccgtccttt tcacgaaggg catggcgacc accgtatccg ggcagtagat
      661 ttcagcaaag gccgcaagtt tgacgggttg cttcacacgc ttcacggcct cttcgaactc
      721 cggggagaaa acggttcttt cgcatgccct tttctgccgg tcccgctctt cctcgtatcc
      781 cttgcggaaa tactcttcca gagaataacc ttttgaaaaa tcgatcattg tgattttcac
      841 ccccgaatgc tggctgaacg ttttctttat tatctcacag aagaaaaaaa accctgccgt
      901 gctatctgct acaattggga aaaaagcgga ggtggaattc catgtccccc tgtagcaagt
      961 gcggcgctga cggtcccttt ctttcccttt ccacgtggag cctgtgtccc gtgtgcaggg
     1021 aagaggccct ttcggacata ctggagtcca tccggaccct tgactgcagg ttcgcctcgc
     1081 tgaaatcccc tccggacggg aaagcctggg cagacttcac gggaaacatg atccgccttc
     1141 tagaccgcct cgccgccatt ctccctgaaa atctggtacc ggaaaacgag cagcccgaag
     1201 accccggacc tttcgccatg gaggaaatcc gcattctcct cggctcaagc gaccttgacg
     1261 aaccccgcct ccgttccgcc gcatccatgc tcgacgggat tcttcccttt gttgagaacc
     1321 gcagccccga aaacttcgct gcgtttctca ggctccgctc aaaagtcttt tccgccctcg
     1381 caaaaagcga ccgggacagg gctatgtccc ttcttgaaaa aaagcatccg tctccggggg
     1441 atcagtccca gctgaccctc atctgacggc ccgggctgcc gaggtcactc cacatcttcc
     1501 cgctccccct tgccggatgc ccctccgtga agattacccc gaaaccagga gaagaaaaca
     1561 ccgaggaaac acaacagaga ggaaatggaa aaactgatcc tcaccacgac gagaaaagtg
     1621 gcgtaccgtt cgggagtgat ctgcccgctc ccgagcaggg cggcgaagac cacggtgatc
     1681 accgccatgc tggtcatctg ccccagtacc ctcatggtgc ctaccgagcc ggaagcggca
     1741 ccgtaaaact tcgatggaac ggaccccatg atggcattgg tgttcggcga ggagaagaag
     1801 gcaaaaccga ggcccagcag aacgagggcc gaaaaaatga aagccatggg cgtttccctg
     1861 ccgagaaagg caaaaacggt cagcccgagg gcagtggaga gcatcccccc cgatgcgagc
     1921 agtcccggtt ctatcctgtc cgacagtttt ccggtgtagg gggagagaaa agcctgcaac
     1981 agaggctggg ttaccatgac aacccccgcc tccgacgggg acataccctt gatgtactga
     2041 agatacagac tcaggaggaa cgccacggaa taggtcgctg catagttgat caatgccgcc
     2101 acgttggaaa aggcgaagac ccggttcccg gaaaaaaggc ggatctcgaa caggggatct
     2161 tcagaccgga gcatcctcat gataaagact gcgaacagcc cccctccggc ggccataagg
     2221 aatacccccc cggccgaggg cagaatggaa gcgccgtaca tgacggccac gagagatgct
     2281 ccatagacga aacaacccgg aaggtcgagc cgtttgctgc tcccctcccg ccattcaaag
     2341 ggaacccgtg ttttcagcag gataatcgag actgccccga gaagggcgtt cacgaaaaaa
     2401 acggaagccc atccgaaggc cgcgatgaga agccctccga agaagggccc cgcagaagac
     2461 ccgagataaa ttgacgcaac attgacgccg agggctttcc cccgctccgc cgccgggaag
     2521 acggaggtca ggatggccat tcccatggtc agcatcagtg aacctcccgc tccctgcacc
     2581 gaccggagca gtatgaagaa cggcattgtg ggggccaggg gacagagaat cgttgaaagg
     2641 atgaatatcc acgttccgag gacaaggatg cgcttccttc cccagatgtc ccccattttt
     2701 cctgccggaa caaggaaaac cgccgatgaa agcaggtatg atgtggccac ccagctcagc
     2761 caaactgcgc tcgcaccgta ggcctgctgt atggagggca gggcgatgtt caccgacgag
     2821 agcatgaagg gcggcagaaa ggcagtgaga gccgcaaccg ccaggacggc attcttcgag
     2881 atgtcgggag actggattcc ggaacggacc atggggcacc acccgctttt tcctgaactt
     2941 gaaaacaata gcataccata tagaatgcat gtgccaaggt ttttcatatt cctgaagaga
     3001 aaaaacggaa gacccggaaa ataaaaaaga ggaaccggcc cgagccgatg cctctttgct
     3061 gttttggtgc cgccgattac cccggaagga gcgtctttcc cgcagcgaag gcccgttcat
     3121 tgatctccac ggcaccggga ggaacacagg accggagtgt tcctccccag tctctgccgt
     3181 ccagccccag ggttttcacc accgctccga gaaggaccat gttcatcact cgcggatttc
     3241 cgagttcatc cgcagatttg gccgcttcca ccacgagagt tccgggagcg gaggcagaaa
     3301 ggttcttttc tatctcccgg ggatattccg cctgtcccag gatcaccggc atggggaaaa
     3361 tctcgtggtt gttcacaatc agccttccgc cggcagagag gtacggaagc catcggaggg
     3421 cctccatttt ttcgaaggcc accaccacgt cggcctctcc cctgcagatg acaggagaat
     3481 acaccttttc cccgtatctc acgtgggtcg acacgctgcc gcccctctgg gccatgccgt
     3541 gaatctccga catcttgacg tcatagccga aggaaaggag tccttccgtc aaaaccttgc
     3601 tcgctagaat ggtgccctgg ccgccgaccc ctacgagcag tacgtttgtg gtctttgtat
     3661 tcatcaagat tcccccttct tcccgacggc gctcttcggg cagacctgga cgcagaggcc
     3721 gcacccgttg caggcctcct ggtcaatgac gatccttccg tcccgcttcg agatggccgg
     3781 gcaaccaagc ttcaggcacg ccccgcagag aacgcaccgt tcttcgtcga cgaagcattt
     3841 cattccggcc cgttccttct gaacattctt cagcagagca caggggcggg tggtgacgat
     3901 gacggaggtt tcttccgccg cccacgccct gtcaacagct tcggcggaag ccttcaggtc
     3961 gtaggggtca acaacgtgaa tattgtcgtc ctttactccg caggccccgc agatcttcgg
     4021 aatatccagc gcgacggtct cctctccgga aagagtcctg cccgatccgg gattttcctg
     4081 ctgtcccgtc atggcggtga tcctgttgtc catgatgacg aacacactcc gggacttgtt
     4141 caccacagcg tcgataagac cggtgatacc ggagtggaag aacgttgaat cgcccaggat
     4201 tccgaaaacc ttccggttcc tgcccgcctt ctcgttcgct ttctcaaagc cgatggcggc
     4261 ggaaaagccc gcccccatgc agatggttgt ctcccccacg ttgaggggag gcatggaacc
     4321 gaggccgtag cagccgatat cccccgtcac gatgacgtct ttctttttgc tcagggggaa
     4381 gaacagcccc ctgtgggggc accccgggca aaggaggggc ggcctgttcg ggggaggaac
     4441 ctccccttcc cgtcccggtg ccggttcttt cccgagaacc gcacgggcca cgatggccgg
     4501 agtgagttcg tccaccacgg gaagaacgtc cttcccgaag caggaaatcc ccatggcccg
     4561 aatctccgtt tcaataaagg ggtcattctc ctccactacc cagagagtct tcactttgga
     4621 ggcaaactcc cggatacgtc tgcgcggcaa aggccatgaa aagccgatct tcaggaagtt
     4681 cgccccgtct cccagtgcct cccgggcata ctgatatccc acgcctgaag ctatcacccc
     4741 gatttccgct ccgtcggcca tcgttacgga attgaagggg gatgattctc cgaattccct
     4801 caaatcctca agcctctttt caacgaggta gtgcttctgc ctggcgaagg cggggagcat
     4861 ggcgtttttc gccgggtttc ttccgtactc cctgacgggg gactcgaccc gatcttccgt
     4921 ctcgaccacg ctcttgctgt ggcatatcct ggtggtgagc ctcaggagaa caggcgtatc
     4981 gaaccgctcg gaaatgtcga aggcggccct ggtgaaatca aggcactcct ggctgtccga
     5041 cggctcgagc atgggtacct tggcatgaag ggcataccac cgattgtcct gttcgttctg
     5101 ggaactggaa agtcccgggt cgtccgcggt gacgacaacc agtccgccgt tcaccccgat
     5161 ataagacatg gtgaaaaagg ggtcggccgc tacgttgagt cccacgtgct tcatggcgca
     5221 cagggcgcgg gctcctgcaa tggacgctcc cgaggcgacc tcgagggcaa ccttctcatt
     5281 gggggaccac tcggaagaca cttcaggaaa ccgcccgagg ttttcgagta tctcagtgct
     5341 gggcgttccg ggataggcgg cagccacatg gcatcctgcc tcccaggcgc cccgggctac
     5401 ggctt
//
LOCUS       DAOTHD010000021         5317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_18376_length_5317_cov_3.131889, whole genome shotgun sequence.
ACCESSION   DAOTHD010000021 DAOTHD010000000
VERSION     DAOTHD010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18376_length_5317_cov_3.131889"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="P5219_00735"
     CDS             <1..298
                     /locus_tag="P5219_00735"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HRX25311.1"
                     /translation="VTILAMDVRRAAGLLRRKNRAFDVIFADPPYGEGWPAVLGPILF
                     PPEGSLLVPGGVVVLEHSTREKMPEGEGWKITDSREYGDTVLTFLSSAGKGGEK"
     gene            295..801
                     /gene="coaD"
                     /locus_tag="P5219_00740"
     CDS             295..801
                     /gene="coaD"
                     /locus_tag="P5219_00740"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRX25312.1"
                     /translation="MKRSINAVYPGSFDPITNGHIYIAERAAALFDEVVVSVLLNPQK
                     KAAFSVEERKAMAREALSHLPNVKVDSFSGLLVDFLRQEQSRIIIRGLRALSDFEYEF
                     QLALMNRQLAPEIETLFIVTEARYSYLSSHAVKEIYQFGGAVHDMVPPGVFRRLRERF
                     PLPRNILP"
     gene            complement(761..2005)
                     /locus_tag="P5219_00745"
     CDS             complement(761..2005)
                     /locus_tag="P5219_00745"
                     /inference="COORDINATES: protein motif:HMM:NF017452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRX25313.1"
                     /translation="MVEIYKKIIGIVAEYNPFHKGHLFHLGKARENKNDAVVVVLSSY
                     FTQRGEPAVMSKWDRAEAALGAGVNLVLELPAFFSCHNAGIFAAGAVDILAATGMVET
                     LSFGMEQPEFDPTPILDILVHEPSHFKDNLKKKLNSGFSYVKARAAALEEIHEGWGAF
                     VSLPNNTLALSYMERILRKGYSISCRPVQRMGSGFHDTDLENTFPSAAAVRKALAEGN
                     REDAEKALPSSTVRILNRCIERGMVVLSREMLWRLIRFLLLRTPAEELARSSEMTEGM
                     ENRFLKYAVLCSSWSGFVSKCTTARYPRGRIQRQLVHFLLGIGHRENRELQSSGPQYI
                     RVLGADAVGMEILRKMRSTAHLPVMGKAPAGLRGEGLLLAGIEQSAANVWEELTAVFS
                     PGEEKKRYPFMEECFSEGENVL"
     gene            2085..3287
                     /locus_tag="P5219_00750"
     CDS             2085..3287
                     /locus_tag="P5219_00750"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HRX25314.1"
                     /translation="MKILVVNCGSSSLKYQLFDMTDESVLAKGLVERIGIEGARIKHT
                     KTGMDSVIRNTDIPNHKVAFKVVVEALLDGNHGVLKSLDELSAVGHRVVHAGEKYAGS
                     VMITQDVIDALEECVPLAPLHNPANLMGIYAVTEALPNIPQVGVFDTAFHQTMPGYAY
                     MYGVPYHYYEKYKVRRYGFHGTSHFYVSGRAAEILGKPVESLNIVTCHMGNGSSIAAV
                     KGGKSMDTSMGFSPLPGVIMGTRSGDVDPLLISFLSDQEGIDNKAMSHVLNKESGLLG
                     ISGVSSDLRDIEEAADAGNQRARLAEEMLYYGVKKYIGSYAAAMGGIDVLVFTAGIGE
                     NSARAREGICSGLEFMGVKLDLEKNNVRGKERIISSDDSRVKVLVVPTDEELVIARDT
                     KKIAIDRN"
     gene            3315..3890
                     /locus_tag="P5219_00755"
     CDS             3315..3890
                     /locus_tag="P5219_00755"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRX25315.1"
                     /translation="MLTEKPASWKFELETPELGNPEAVTYLHAEAETGQFDGIFYDGM
                     RFSFEGPVIVDAEGRWIDSDLSVSLKISATFSAPCSRCLEPSRIEILNDFLYLYSLRK
                     KESPEESSEDGNFHEVQVSRWQRFFDISDHVWESIIVSLPGKVLCSADCKGLCPQCGR
                     PLRNGDCGCSAKELDPRLEKLLGIKIEETKE"
     gene            3912..4115
                     /gene="rpmF"
                     /locus_tag="P5219_00760"
     CDS             3912..4115
                     /gene="rpmF"
                     /locus_tag="P5219_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRX25316.1"
                     /translation="MATPKSRVSHARTHKRKSEWIGALTAPEVTTCPHCGEVIQNYRA
                     CGECGFYRGRQVVKTASEEKKEA"
     gene            4252..4836
                     /gene="fapR"
                     /locus_tag="P5219_00765"
     CDS             4252..4836
                     /gene="fapR"
                     /locus_tag="P5219_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045717 - negative regulation of fatty acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HRX25317.1"
                     /translation="MEGTSIKSQRKKNRQDRLLKLIEQNPLATDEELAASLEASVSTI
                     RLDRAVLGVPELRERMRSMAQKATSRLRSLKQSEVVGDLLELEPNKWALSVLETRRDM
                     AFRKTDMVWDHYIYAQASSIAIAVVEADLVIVDSMRGEYKGHARVGDSLVARAKVGVQ
                     KDGKYIVSVRTKVGEKEIFVGRFIAAIVSQNEPV"
     gene            4858..>5317
                     /locus_tag="P5219_00770"
     CDS             4858..>5317
                     /locus_tag="P5219_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRX25318.1"
                     /translation="MILALDAMGGDHGPSEVCRGAIAACEEHSDLKLVLVGNSPEIEP
                     MLDGASRDVSGRISIVHAEERIGMDEHPTVAIRKKRHSSLRIAMEMVRSGEASGCVSA
                     GNTGAIVAGGVLVVGRISGIDRPALGVALPTLNKYTFLLDVGATVRSKPEN"
BASE COUNT         1288 a         1399 c         1479 g         1151 t
ORIGIN      
        1 tgttaccata ctggctatgg atgtcaggag agcagcaggt ctcctccgaa ggaaaaatcg
       61 cgctttcgac gtgattttcg cagacccgcc ctacggggaa gggtggcctg ccgttctcgg
      121 cccgattctc tttccccctg aaggatccct tctcgtcccg gggggagttg tggttttgga
      181 acattcaaca agggagaaga tgcccgaagg ggaaggatgg aagattaccg acagccgcga
      241 atacggagac acggtcctga catttctctc gtctgccggg aaagggggag aaaaatgaaa
      301 cgatccatca acgcggtgta ccctggttcc tttgacccta taacgaacgg gcacatttac
      361 atcgcagagc gtgcagccgc tctttttgac gaggttgtgg tcagcgtcct gctcaatccc
      421 caaaaaaagg cggccttttc ggtggaagag cgaaaggcaa tggcccggga ggctttgagc
      481 catcttccca acgtgaaggt cgattctttc tccgggctcc tcgtggactt tctgcggcag
      541 gaacaaagcc gcatcatcat aaggggactc agggcccttt ccgacttcga gtacgaattc
      601 cagctcgccc ttatgaacag gcagcttgcc ccggaaatcg agaccctgtt cattgttacc
      661 gaagcccggt attcatacct gtcgagccat gcagtgaagg aaatatacca gttcggcgga
      721 gcagttcacg acatggtccc ccccggggtc ttccggcgtc tcagagaacg ttttcccctt
      781 ccgagaaaca ttcttccatg aagggatagc gtttcttttc ctcccctgga gaaaagacag
      841 ccgtcagttc ttcccagaca ttggcagccg actgttcgat gccggcgagg aggagccctt
      901 ctcccctcaa gcctgccgga gctttcccca tcaccggaag atgtgccgtt gacctcattt
      961 tccggagaat ctccatgccg acagcatccg ccccgagaac ccgtatgtat tgggggcccg
     1021 agctctggag ttccctgttt tcacggtgac cgatcccaag aaggaaatgg accagctgcc
     1081 gctggattct gccccgggga tagcgggccg tggtgcattt cgagacgaac ccggaccagg
     1141 aagaacaaag gacggcgtat ttcaagaacc tgttttccat cccttcggtc atttcggatg
     1201 accgggccag ttcttccgcc ggggtcctca aaagaaggaa gcggatcagc ctccagagca
     1261 tttccctgga gagaaccacc atacctcttt cgatgcaccg gttcagtatc ctgacggtag
     1321 aggagggaag agccttttca gcatcttccc gattgccctc cgccagggct tttctgactg
     1381 cggcagcgct cgggaaggtg ttttcgagat ccgtgtcatg aaaaccggat cccatccgct
     1441 ggacaggtct gcaggaaata ctgtatccct ttctcaggat cctctccatg taagagaggg
     1501 ccagagtatt gttcggcagc gaaacgaaag ccccccatcc ctcgtggatt tcttccaggg
     1561 ccgcagcccg ggctttgacg taggaaaatc ccgaattcag cttttttttc agattatcct
     1621 tgaaatgtga gggttcgtgc actagaatat caagaatcgg ggtcgggtcg aattcgggtt
     1681 gctccattcc aaaagaaagg gtctcaacca ttcctgtggc ggcaagaatg tccacagcgc
     1741 cggcggcgaa aatgccggcg ttatggcagg aaaaaaaagc gggcagttcg agcacgagat
     1801 tgacccccgc cccaagagcg gcttccgcac ggtcccattt gctcataacg gcaggctcgc
     1861 cccgttgggt aaaatatgaa gacagcacga cgacaacggc gtcgttcttg ttttcccttg
     1921 cctttccaag atggaaaaga tgccccttgt gaaacgggtt atattcggcc acaataccta
     1981 ttattttttt atatatttcg accatccgcc tttctccggc tttctccata agatacagcc
     2041 gggaagaggc ggcggtcaag aagaagagag gaaggggtcg cgcaatgaaa attctggtgg
     2101 ttaactgtgg aagttcatcc cttaagtatc agctttttga catgaccgac gaatcggtcc
     2161 tcgccaaggg actcgttgag agaataggca tcgagggagc caggatcaaa cacacgaaaa
     2221 ccggcatgga ttcagtgatc aggaataccg atattccaaa ccacaaggtg gccttcaagg
     2281 ttgtggtcga agcgttgctc gacggcaacc acggagttct gaagagcctc gacgaacttt
     2341 ccgccgtggg acacagagtg gtgcatgccg gtgaaaaata tgccggctcg gtcatgatca
     2401 cccaggacgt catcgatgcc cttgaagaat gcgtgcccct cgcgcctctt cataaccccg
     2461 caaatctcat gggcatttac gccgttaccg aagcccttcc aaacattccc caggtgggcg
     2521 ttttcgacac cgccttccat cagaccatgc ccggatacgc gtacatgtac ggcgtcccct
     2581 accattatta cgagaagtac aaggtccgcc gctacggctt ccacggcaca agccacttct
     2641 acgtttccgg aagagccgcg gaaatcctcg ggaaacccgt ggaatccctc aacatcgtaa
     2701 cctgccacat ggggaacggc agttccatcg ccgccgtcaa gggagggaaa tccatggaca
     2761 ccagcatggg attcagccct cttcccggag tgatcatggg cacgagatca ggagacgtgg
     2821 atccgctcct catttccttc ctctcggacc aggaaggaat tgacaacaag gccatgagcc
     2881 atgttctcaa caaggaaagc ggacttctgg gaatttccgg cgtgagcagc gacctcaggg
     2941 acatcgagga agcggcggat gccggaaacc agagagcgcg gcttgccgag gaaatgctct
     3001 attacggtgt gaaaaagtac atcggatcct atgctgctgc catgggcgga atcgacgtac
     3061 tggtcttcac cgccggcatc ggcgaaaaca gcgcccgggc ccgggaaggt atctgctccg
     3121 gccttgagtt catgggagtc aagcttgacc tggagaaaaa caacgtccgc ggcaaggagc
     3181 gcatcataag ctccgacgac agccgggtaa aggtcctcgt agttccaacc gatgaagaac
     3241 tcgtcatcgc cagggatacg aagaagatcg ccattgacag gaactagcgg attacttcct
     3301 ggaggccctt cggaatgctg acagaaaagc cggcttcatg gaaattcgag cttgaaactc
     3361 cggaactcgg caaccccgaa gccgtgacat atctgcatgc cgaagccgaa accgggcagt
     3421 tcgacggaat tttctacgac ggcatgaggt tcagcttcga aggtccggtc attgtggatg
     3481 cagaaggccg ctggatcgac agcgatcttt cggtttcatt gaagatttcc gcgacttttt
     3541 ccgctccatg ttcacgatgt cttgaacctt cacgtattga aattctgaac gatttcctgt
     3601 atctttattc attgcggaag aaagagtctc cagaggaatc ttccgaggat ggaaatttcc
     3661 atgaagtaca ggtgtcgaga tggcagcgtt tttttgatat ctcggatcat gtgtgggaga
     3721 gcattattgt atcccttccc ggcaaggttc tttgttcggc tgactgcaag ggcttatgtc
     3781 cccagtgcgg gcgtcctctc aggaacggcg attgcggttg ttcagcgaaa gaacttgatc
     3841 cgaggctgga aaagcttctc ggcataaaaa ttgaagaaac gaaagaatag cgaaaaggag
     3901 ggaagtccag aatggcaacg ccgaaaagca gggtatccca tgcccgtact cataaaagaa
     3961 agtccgagtg gatcggggct cttactgctc cggaagttac tacgtgcccc cattgcggtg
     4021 aggttatcca gaattaccgg gcctgcggtg agtgtggttt ttaccggggc agacaggtag
     4081 tcaaaacggc ctcggaagaa aaaaaagagg cttgatcgga agaaaccggg caagaggaag
     4141 ggaaaccttc ctcttttttt tattttcccc ttccctccat attgaccgtt cggccacggg
     4201 gtactatact ttccacatct ttaagagcag atactatcat caggtgccaa aatggagggg
     4261 acatccatta agtctcagag gaagaaaaac aggcaggaca ggctcttaaa acttatcgaa
     4321 caaaatccgc tggccacaga tgaggaactc gcagcctcgc tcgaggcaag cgtgagcacc
     4381 atcaggctcg acagagccgt tctgggtgtc ccggagttga gggagagaat gcgctccatg
     4441 gcccagaaag ctaccagcag gcttcgctcg ctgaaacaga gtgaagtggt aggggatctt
     4501 cttgagctcg agccgaacaa gtgggccctt tcggtgctcg agacccgcag ggatatggct
     4561 ttccggaaaa cggacatggt gtgggatcac tatatttacg cccaggccag ctccatagcc
     4621 attgccgttg tggaagccga tctggtcata gtggattcca tgcggggtga atacaaaggg
     4681 cacgcgagag tgggagattc cctggtcgca agggcaaagg tcggtgtcca gaaagacgga
     4741 aagtacatcg tcagcgtgag gacaaaggtc ggtgaaaaag aaatcttcgt ggggagattt
     4801 atcgctgcta ttgtttcgca aaacgaaccg gtgtagcgtg agagaggtga acttcatttg
     4861 attcttgccc tggatgccat gggcggtgac catggccctt cagaagtgtg caggggagcc
     4921 atagccgcct gcgaagaaca ctctgatctc aaacttgtac ttgttggaaa cagcccggaa
     4981 attgaaccta tgctcgacgg ggcttcccgg gacgtttccg ggagaatatc cattgtccac
     5041 gccgaagaaa ggatcggcat ggatgagcac cctacggtcg ccatccggaa gaaacgacat
     5101 tcgagtcttc ggatagccat ggaaatggtc cgcagcggcg aagcttcggg gtgtgtttcc
     5161 gccggaaaca cgggagccat cgtcgcgggc ggagtgcttg tcgtgggaag aatttcggga
     5221 attgaccgcc ctgcccttgg tgtggcgctg ccaaccctca acaagtacac cttcctgctc
     5281 gacgtggggg ctaccgtacg gagcaagcct gaaaacc
//
LOCUS       DAOTHD010000022         5300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_18460_length_5300_cov_2.861010, whole genome shotgun sequence.
ACCESSION   DAOTHD010000022 DAOTHD010000000
VERSION     DAOTHD010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18460_length_5300_cov_2.861010"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="P5219_00775"
     CDS             <1..379
                     /locus_tag="P5219_00775"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRX25319.1"
                     /translation="ARFLKNREAGKGGVWVADHNGTVVGSIAIDHEDDTTAKLRWLLV
                     HPEFRGMGIGRRLMDEAMEFCRLNLYKRIFLWTFSELREARHLYEMYGFSPTEEAKHV
                     IWGREITEERWDYLLFDPEEEEA"
     gene            493..1605
                     /locus_tag="P5219_00780"
     CDS             493..1605
                     /locus_tag="P5219_00780"
                     /inference="COORDINATES: protein motif:HMM:NF025386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HRX25320.1"
                     /translation="MGIRFRKTITIAPGIRINLSKSGVSATVGPKGATLGIGRGGVHA
                     NVGIPGSGIFYRKKLPIPWPGGTKKSGPAAGSPQADEGRNAAEEMNSTVQEIIDIHLR
                     TLPPSAAPAYTPREYGEPVPGLPPAPFPLWLRGAVLAAALVLLYFTWGLSFIPWALWE
                     GYIYYVRKKGHSLLLAEWEERKKAFEQEEARRAVRFEEALQLSGEKPEAALEAVISSI
                     EWPRETHVSYQVDGTIAFLDVDLPEIEDMPQVVYGFSKRSGLTESPKSETQIRKDFAR
                     HVHGVAFLLLGEVFRTIPSITEIVLSGYTQKIQPSTGREEDQYLYSLRVHRDQWKDIN
                     FSALSDIDPVEAIGGFEIRRNMTKTGIFKTVEPFSL"
     gene            1630..2238
                     /locus_tag="P5219_00785"
     CDS             1630..2238
                     /locus_tag="P5219_00785"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRX25321.1"
                     /translation="MKYYDITRELLSSPVYPGDREPELETVFDMHRGADFNLHSLSCS
                     LHAGTHCDAFLHFCRDGSDIASIPLEHFIGPCLVLSVPENRLVTEDDLRNRLPEGTTR
                     LLLKSGGTSFLALSGAAFLLRRGIFTLGTDASSVAPPDNEAGIHIPLLTGGVALIESL
                     WLEEVPDGNYFLSAAPLKIRGAEGAPCRAVLFVPEEEPPEIP"
     gene            2256..3854
                     /locus_tag="P5219_00790"
     CDS             2256..3854
                     /locus_tag="P5219_00790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HRX25322.1"
                     /translation="MIFKSVRRWAENHPFIFAPGFVGAATLLFLPGRELLGKGHWALL
                     YLLVISVVASVAGSRPSFLAALSAFLAWDVFFLPPYNTFRVDDPKDWISLGVFLVVGV
                     LIGLTSGRLREREALAVAQEREMALLNRLTGRLLAIQKPLDMAETLCAEIEAVFSPRF
                     IRFHLTDSAKNRALLFGMRNDLDEDPLEEDFLRETAERSVGIASPAFEPSRPAPGEES
                     GIIRHSGLFPSVSRNDVFLPVLSSSRLEGILHIGERRSERNYSWNEIQLLSAAAHMAG
                     TFLERNRLDESARQAETLKEGDKLKSAIFSSVSHELKTPLSSLTATISNILESDRTGD
                     PEHVKKELGSVKKDLDRLSHSIGSLLDLSRLAAEAWKPDLQWVEPGEVLGWVLFRLER
                     NDRERVKAELPENLPLILVDFQQWGRMLMHILENALSYSPADSPVTVGAERSASGLDI
                     RISDNGPGIPDDEKRMIFTKFFRGIHSGFLPGGTGLGLPIALEIARVHEGTIRVEDNR
                     PSGTVFIISLPSGHVREETEDDLS"
     gene            3851..4549
                     /locus_tag="P5219_00795"
     CDS             3851..4549
                     /locus_tag="P5219_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRX25323.1"
                     /translation="MSSPERSIRILVIDDEESIRRALQSILSLRKYDVTLASGGADGI
                     EKAIETNPELVILDLSLPDMDGLDVCRELRTWMNSPILILSVRGNESDKVQALDLGAD
                     DYLTKPFQAGELLARIRAILRRAESRIIQPPEIVAGDLTINLAKRQLLRQEQEISLTR
                     TEFEILSLLAQNIDCVVTYRMLSERIWDDREERDPSLLRVHVSNLRKKIEPDPSLPRY
                     IHTEPGVGFRFSSQ"
     gene            4692..4874
                     /locus_tag="P5219_00800"
     CDS             4692..4874
                     /locus_tag="P5219_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25324.1"
                     /translation="MLCLGDLAEKHCSFSFDENGCLRLFFSDHSVVLYKDDDVIVFAG
                     DGDLSPSEAERWVKTH"
     gene            4967..>5300
                     /locus_tag="P5219_00805"
     CDS             4967..>5300
                     /locus_tag="P5219_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRX25325.1"
                     /translation="MKKEEKVVLVVGLGRFGQSLCKTLSEFHTQVIAVDTDIECVEET
                     AGLVELCVQADATDADALAKCGAKEADIAVVAIGESVEASILATTILKDLGVPYVIAR
                     AENDIHARV"
BASE COUNT         1154 a         1652 c         1490 g         1004 t
ORIGIN      
        1 ggcacggttc cttaaaaaca gggaggccgg aaagggaggc gtctgggttg cggaccacaa
       61 cggcaccgtt gtcggctcca tagccatcga ccacgaggac gacacaacgg ccaagctccg
      121 ctggcttctc gtccacccgg aattccgggg catgggcatc ggaaggcgcc tcatggacga
      181 agccatggaa ttctgcagac tgaacctcta caagcgaatc ttcctgtgga ccttttcgga
      241 actccgggag gcccgccacc tgtacgaaat gtacggtttc tcccccaccg aggaagcgaa
      301 gcatgtcatc tggggaaggg aaatcaccga agaacggtgg gactatctgc tctttgaccc
      361 ggaggaagag gaagcgtaga cttcgtttcc atgtttcggg ggacggatcc ggcacagaaa
      421 ccggaccttt gagctttctg cagacaaacg tgaacatcgt ccccgcaacg gaaggaggat
      481 gagaagggca tcatgggcat ccgctttcgg aaaaccatca ccatcgcgcc cggaatcagg
      541 atcaacctga gcaagagcgg cgtcagcgcc accgtcggcc cgaagggagc caccctggga
      601 ataggcaggg gaggcgtcca tgccaatgtg ggcattccgg ggtcgggcat cttctaccga
      661 aagaagctcc ccattccctg gcccggcggc acgaaaaaat ccggcccggc agcgggaagc
      721 ccgcaggccg atgagggaag aaacgcagcg gaagagatga acagcaccgt ccaggagatc
      781 atcgacatcc acctccggac ccttccgccc tcggccgccc cggcctatac cccccgggaa
      841 tacggcgagc ccgttcccgg cctgcccccc gcccccttcc ccctctggct gcgaggagcg
      901 gtcctggcgg cggctcttgt cctcctctac ttcacgtggg gactctcctt catcccctgg
      961 gcgctgtggg aaggatacat ttactatgtc aggaagaagg ggcacagcct gctcctcgct
     1021 gaatgggagg aacggaaaaa agcctttgaa caggaggaag cccggcgggc cgtccgcttc
     1081 gaagaagccc tacagctctc cggagaaaag cccgaggctg ccctcgaggc ggtgatctcc
     1141 tccatcgaat ggcccaggga aacccatgtc tcctaccagg tggacgggac cattgccttt
     1201 ctcgatgtgg accttcccga aatcgaagac atgccccagg tggtctacgg attctcgaaa
     1261 cgctccggcc tcacggagtc gccgaaaagc gaaacccaga tacggaagga ttttgcacgc
     1321 cacgtccacg gcgtcgcctt cctgcttctc ggggaagtct tccggaccat cccgtccatc
     1381 accgaaatcg ttctttcggg gtacacccag aagatccagc cctccacggg gcgggaggaa
     1441 gaccagtatc tttacagcct tcgggtccac agggaccaat ggaaggacat caacttctcc
     1501 gccctgtcgg acatcgaccc cgtggaagcc atcggcggct ttgagatccg ccggaacatg
     1561 acgaaaacgg gcatcttcaa aactgtggag cccttttccc tgtaagggac cggagaggca
     1621 gccccgttca tgaaatacta cgatatcacc cgggaactgc tctcgtcgcc ggtctacccc
     1681 ggcgaccggg aaccggagct ggaaaccgtc ttcgacatgc acaggggagc ggacttcaac
     1741 ctccactccc tttcctgcag tcttcatgcc ggcacccact gcgacgcttt tcttcacttc
     1801 tgccgcgacg ggagtgacat cgcctcgatt cccctcgaac actttatcgg gccatgcctc
     1861 gtcctttccg ttccggaaaa cagactcgtc acggaagacg acctcaggaa ccgactccct
     1921 gaaggaacga cgcggctgct gctgaaatcg ggaggtacat ccttcctcgc actgtcgggt
     1981 gcggcatttc tgctccgccg gggaatcttc accctcggca cggacgcctc gtcggtggcc
     2041 ccgccggaca acgaagcggg cattcatatc cctctcctca cgggaggcgt ggccctcatc
     2101 gaatccctgt ggctggaaga agttcccgac ggaaactact tcctctcggc tgcccccctc
     2161 aagatcagag gagcggaagg ggcgccgtgc cgggccgtgc tgttcgtccc ggaagaggaa
     2221 ccaccggaaa tcccgtaatg ccggcggagg gaaaaatgat cttcaagagc gtgagacgct
     2281 gggcggaaaa ccacccgttt attttcgctc ccggtttcgt cggagcggcg accctgctgt
     2341 ttcttcccgg gcgggaactc ctcggcaagg gacactgggc ccttctctac cttctggtga
     2401 tttccgtggt cgcctcggtg gcaggatccc ggccctcctt cctggcagct ctctccgcct
     2461 tcctggcctg ggacgtgttt tttcttcccc cctacaatac cttcagggta gacgatccca
     2521 aggactggat ctccctcggc gtcttccttg tggtgggagt actcatcggt ctcacctcag
     2581 gcagactgag agaaagggag gctctggcag tcgcccagga aagggagatg gccctgctca
     2641 acaggctgac aggacgtctt ctcgccatac aaaagccctt ggacatggcc gaaaccctct
     2701 gcgcggaaat cgaggcggtc ttctcccccc ggtttatccg tttccacctt acggacagcg
     2761 caaaaaacag ggccctcctc ttcggaatga gaaacgacct cgacgaagac cccctcgaag
     2821 aggatttcct cagggaaaca gcggaacggt ccgtcgggat cgcttctccc gccttcgagc
     2881 cgtcccgccc cgcaccgggt gaggaaagcg gaatcatccg ccattccggc ctgttcccct
     2941 ccgtctcgcg aaacgacgta ttccttcccg tgctgtcttc cagcaggctc gaaggcatac
     3001 tgcacatcgg ggaacggagg agcgaaagaa actacagctg gaacgaaatc cagcttctgt
     3061 cggcggcggc ccacatggca ggaacattcc tggagaggaa caggctcgat gaatcggccc
     3121 gccaggctga aaccctcaag gaaggggaca agctcaagtc ggccatcttt tcctcggttt
     3181 cccacgaact gaaaacgccc ctttcctcat tgacggccac catttccaac attctggaga
     3241 gcgacaggac aggtgatcct gaacacgtaa aaaaggagct cggatcggtc aaaaaggacc
     3301 tggacagact atcccactcc atcggctccc tcctggacct ttcgagactc gccgccgaag
     3361 cgtggaaacc ggatcttcag tgggtggaac ccggagaagt cctcggctgg gttctcttcc
     3421 ggctggaacg gaacgaccgc gagcgggtca aagcggaact gccggagaac cttcctctca
     3481 ttctggtgga cttccagcaa tggggcagaa tgctgatgca catcctggaa aacgccctct
     3541 cctacagccc cgcggacagc cccgtcaccg tcggagctga aagaagcgcg tcgggcctcg
     3601 acatccgcat cagcgacaac ggcccgggga tacctgacga cgaaaaacgc atgatcttta
     3661 ccaagttctt ccggggaatc cattcggggt ttcttcccgg cggaacggga ctcggactgc
     3721 ccatcgccct ggaaatcgcc cgggtccacg agggaacgat ccgggtcgag gacaataggc
     3781 cttcaggaac ggttttcatc atttccctgc cttcaggcca cgtcagggag gaaacggagg
     3841 acgacctctc atgagctccc cggagagaag catcagaata ctggtcatcg acgacgaaga
     3901 atccatcagg agggccctcc agtccattct gtccctgcgg aagtacgacg taacccttgc
     3961 ctccggcggc gccgatggga tcgaaaaggc catcgagacc aacccggaac tcgtcatcct
     4021 cgacctctcc cttcccgata tggacggcct tgatgtctgc cgggaactgc gcacctggat
     4081 gaactccccc atcctgatcc tctcagtcag ggggaacgag agcgacaagg tgcaggccct
     4141 tgacctgggg gccgatgact atctcacgaa acctttccag gcgggggagc tgctcgccag
     4201 gatacgggcc attctccgtc gggcggaatc gaggattatc cagcctccgg agatcgttgc
     4261 aggcgacctg accatcaacc tggcaaaacg acagctcctg cggcaggaac aggaaatttc
     4321 ccttacgagg acggaattcg agattctttc cctcctcgcg cagaacatcg actgcgtggt
     4381 aacctaccgc atgctgagcg aaaggatctg ggacgacagg gaagaacggg acccatccct
     4441 tcttcgcgtc cacgtgagca acctcaggaa aaagatcgaa ccggatccct cccttccgcg
     4501 gtacatccat acggaaccag gagtgggatt ccgcttctcc tcccagtgat cccctttctt
     4561 tattaaaaat ttacgcctgc ggcttctttc ttgacggtgt cttaatttac cctctggtag
     4621 tctgtaccgg gtgaatcccg ccatgccgga aaaatacggc gggaaatcgt gaaaggaagg
     4681 agatgggaag gatgctgtgt cttggtgatc ttgccgaaaa gcactgctct ttctccttcg
     4741 atgaaaacgg ctgccttcgc ctgtttttct cggatcattc cgtcgttctg tataaagacg
     4801 acgatgtcat agtcttcgcc ggggatgggg atttatctcc gagcgaggcc gagcggtggg
     4861 tcaaaaccca ctgaagccca acggccgaag cggcgcctga atccggccat ctcccgcagg
     4921 atgggtgcgc cggtatcttc tcactcgact ccctggagga atgaacatga aaaaagaaga
     4981 aaaagtggtg ctggtcgtcg gattgggccg gttcggccaa tctttgtgca aaacgctctc
     5041 agagttccac acccaggtca tagcggtgga caccgatatt gaatgcgtcg aggaaaccgc
     5101 cggtctcgtt gagctgtgcg tgcaggctga cgcaaccgac gccgacgctc ttgccaaatg
     5161 cggcgccaag gaagccgaca tcgccgtggt cgccatagga gaaagcgtcg aggccagcat
     5221 tcttgccacc accatcctga aggatctcgg tgttccctac gttatcgccc gggcggaaaa
     5281 cgacattcat gcccgggttc
//
LOCUS       DAOTHD010000023         5288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_18512_length_5288_cov_3.022167, whole genome shotgun sequence.
ACCESSION   DAOTHD010000023 DAOTHD010000000
VERSION     DAOTHD010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18512_length_5288_cov_3.022167"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="P5219_00810"
     CDS             <1..627
                     /locus_tag="P5219_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRX25326.1"
                     /translation="GASVTMGSGFFLAHKAAGTERPIVSTIGDSTFFHMGIPGLVSAV
                     YNRHAFVLGILDNSTTAMTGGQANPAVGDKLRKGDEGRKVDIEAVCRGCGVTFVRTIE
                     AYDVAAGKEAVKEAWEHAQSRSEPAVIIFRHPCMLLRPQQPVVQVKVDPEKCIGCKFC
                     INFFNCPGLVFDETRSKAYIDERFCVSCGVCISVCPHGAIAEVSEEGK"
     gene            629..1192
                     /locus_tag="P5219_00815"
     CDS             629..1192
                     /locus_tag="P5219_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRX25327.1"
                     /translation="MQYVIVGIGGQGILFASKVLGKIALERGESVIGSEVHGMAQRGG
                     SVISHFKVGDYRSPLVMAGEADILLAFDQSEAVRNLHFLKKKGSLVVNVHDRKALENA
                     SLAGLVRDREINVHSLEGYALPKEHMGGNFLFLNVLLMGAMCGAGISGLSFAEVSRAV
                     KELSPPKFQDANMKVLALGAEAICSGR"
     gene            1195..1710
                     /locus_tag="P5219_00820"
     CDS             1195..1710
                     /locus_tag="P5219_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25328.1"
                     /translation="MAGSERGGRSRSPLFRGRASSLAELLAVLAICLVLGGGMSLAVV
                     SSFAGPASDTTAVREGKYAAQWLQRIFHKALLSGRSFVFRLSTSVPQNRLKIQWADGR
                     EETYDGKGRVWFTNHSGVPAFCAYSPKWNTISPAFTIEAGSSPDRRRPLKYIVVTPFC
                     RVSYRDDPPRD"
     gene            1798..2340
                     /gene="lepB"
                     /locus_tag="P5219_00825"
     CDS             1798..2340
                     /gene="lepB"
                     /locus_tag="P5219_00825"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRX25329.1"
                     /translation="MAAAAKPWWRETIETVLWALVLALVLRTFVVQAFWIPSGSMIPT
                     LEVGDRVLVAKFWYHFTEPKRGQLFVFKYPVDPKRDFVKRIIGLPGDVLEIRGGMVYV
                     NGQPLREEYVKNRDSFSLTPGPVFSEVPVKVPEGHFFAMGDNRPNSQDSRFWGFVPKQ
                     NIRGPVFFRYWPLNRIGMVD"
     gene            2341..3171
                     /locus_tag="P5219_00830"
     CDS             2341..3171
                     /locus_tag="P5219_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HRX25330.1"
                     /translation="MGRTVWYPGHMAKGKRKLAELAEKLDIILEVRDARAPVVTSSPL
                     SEQLSRICPVAVVLSKKDLADGKGTAKWLSWFTSSGRKAWAVNLLKPSMDQIRKDLAP
                     FGPSHREVRLAVVGIPNVGKSMFLNALVGKSSAQVGGIPGITRGVSWYKGKGFLAVDS
                     PGILDPRSGDSVQRCLAWLGSSKSEVIGGYDLIALDLISVLRRRNLWVMVEEKWGVPA
                     PEELPGEEILEALGRRLGCLVAGGAVDILSAGRKFVEAFSTGRLGPVTLELPGDSPWI
                     "
     gene            3162..3746
                     /locus_tag="P5219_00835"
     CDS             3162..3746
                     /locus_tag="P5219_00835"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRX25331.1"
                     /translation="MDLSRWGRIAGADEAGRGPLAGPVVAAAAVLTPAQEEELLSLGL
                     TDSKKLTPRRREFLFERMLFLKVRWRAQAASPARIDGMNILRASLWAMGRSVEKLPPC
                     FDTVIVDGTFPLPGLSCPQVPLPKADSLIPAAAAASVAAKVLRDRIMDILDGLYPGYG
                     FKKNKGYPTVAHRKALEALGPSAVHRAGFTWRAP"
     gene            3746..4801
                     /locus_tag="P5219_00840"
     CDS             3746..4801
                     /locus_tag="P5219_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25332.1"
                     /translation="MAAQGIGGISGPSVRPGHPEGQTQPRVDGSPRQPGTQLPRIADG
                     TVVNGLVLEAREEGSYLVRVAGQALLARANLPLLPGQHFRAVWDASGDIPVLRLSDAE
                     AALLGKLPAGDREVASALLSRGLPLDSRALASLRSAWSGMGGQPSQLAPLAELWARGI
                     PLTAGNVQVLAWYFTLGENEAASLWKKVRDEIRSRAARGENPLAILKEMTGGDDERGA
                     FLRGHALLSRPSREGIDPSLLAPALLPAGDGEGTLTARITTGAWKRQGKSFWSISFEM
                     EGNRIGPVGGDVESDGKNLGVTLKAERSETFEALRMRRHLLRQELGSLPLALQHLAVA
                     RGRRVPRIPGRGLDITV"
     gene            4798..5076
                     /locus_tag="P5219_00845"
     CDS             4798..5076
                     /locus_tag="P5219_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HRX25333.1"
                     /translation="MNGKGKKRLQAAAVQYDEKHDAAPRVTASGKGYVAEKILELARE
                     ADVPVVEDAALVSALMVLELGEEIPAELYEAVARILAFIYKLDKGEKP"
     gene            5073..>5288
                     /locus_tag="P5219_00850"
     CDS             5073..>5288
                     /locus_tag="P5219_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRX25334.1"
                     /translation="MTAEHLERGRRGEEIAAAYLAGLGWTILDRNVVFRGGELDLVAL
                     SGGELVVVEVRTRSEGWMQRGEESVGPR"
BASE COUNT          941 a         1628 c         1751 g          968 t
ORIGIN      
        1 ggagcctcgg tgaccatggg atccgggttc ttcctcgccc acaaggctgc cggaacggaa
       61 cggcccatcg tgagcaccat cggcgattcc accttcttcc acatgggtat tcccggcctc
      121 gtgagcgcgg tctacaacag gcacgccttc gtcctcggca tcctggacaa cagtaccacc
      181 gccatgacgg gcgggcaggc gaaccctgcg gtgggcgaca agctcaggaa gggcgacgaa
      241 ggccggaagg tggatatcga ggccgtctgc aggggctgcg gcgtgacctt tgtccggacc
      301 atcgaagcct atgacgtcgc cgcggggaaa gaggccgtga aggaagcctg ggagcacgca
      361 cagtccaggt cggaaccggc tgtaatcatt ttccggcacc cctgcatgct cctccgtccc
      421 cagcagcctg ttgtccaggt caaggtggac ccggaaaagt gcatcggctg caagttctgc
      481 atcaatttct tcaactgccc cggcctcgtc ttcgacgaga cgaggagcaa ggcatacatc
      541 gacgagcggt tctgcgtttc ctgcggggtc tgtatctctg tctgccccca cggggccatc
      601 gcggaagttt ccgaggaggg gaaatagcat gcagtacgtc atcgtgggaa tcggcggcca
      661 gggtattctt ttcgcgagca aggtcctcgg aaagatcgct ctcgagcggg gcgagtccgt
      721 catcggcagc gaggtgcacg gcatggccca gcggggaggg tctgtgatca gccacttcaa
      781 ggtgggggat taccggagtc ccctcgtcat ggcgggcgag gctgacatcc tcctcgcctt
      841 cgaccagagc gaggcggtgc ggaacctcca cttcctgaaa aagaagggaa gtctcgtggt
      901 gaacgtccac gaccggaaag cacttgaaaa cgcctcactc gccgggctgg tccgggacag
      961 ggagatcaat gtccactccc tggaaggata cgccctgccc aaggagcaca tgggggggaa
     1021 tttcctcttc ctcaacgttc tgctcatggg ggccatgtgc ggggcaggaa tcagcggcct
     1081 ctccttcgca gaggtctccc gggcggtgaa ggagctttct cctcccaagt tccaggatgc
     1141 caacatgaag gtcctggctc tcggtgccga ggcgatctgc agcgggaggt aatcatggcg
     1201 ggcagcgaaa ggggaggccg gtcgcggtct cccctttttc ggggcagggc ctcctccctt
     1261 gcggaactgc tggcggtcct ggcgatctgt cttgtcctcg gaggcgggat gtctcttgcc
     1321 gtcgtctcat ccttcgccgg gccggcttcc gataccacgg cggtccggga gggcaagtac
     1381 gccgcccaat ggctccagcg gatcttccac aaggccctgc tgtccggcag gtcttttgtc
     1441 ttcagacttt ccacgtccgt gccccagaac aggctgaaga tacagtgggc ggacggaagg
     1501 gaggaaactt atgacggaaa ggggcgggtg tggttcacga accacagcgg agttccggcc
     1561 ttctgcgcct actccccgaa gtggaacacc atttctccgg cgttcaccat cgaagccggt
     1621 tcgtcaccgg accgcaggcg ccccctgaaa tatatcgtgg tcactccctt ctgcagagtt
     1681 tcttaccggg atgatccgcc ccgggattga aagacacctt ctttcggggt ataaagagaa
     1741 accggcgttc ccgctttttt ttttcaggaa cgcacatcgg aaacggaggt tgtcctgatg
     1801 gcggcagcgg caaaaccatg gtggagggag acgatcgaga cggtgctgtg ggcgctcgtc
     1861 ctcgctctcg ttctccgtac atttgtagtt caggcgttct ggattcccag cggatccatg
     1921 atccccaccc ttgaggtggg cgaccgcgtc ctcgtggcga aattctggta tcacttcact
     1981 gagccgaagc gggggcagct ttttgttttc aagtaccccg tggatcccaa gagggatttc
     2041 gtgaaaagaa tcatcggtct tcccggtgac gttctggaga tccggggagg catggtgtac
     2101 gtcaacgggc agcccctccg ggaggagtac gtgaagaacc gcgattcctt ctccctgacg
     2161 ccgggccccg ttttttcgga ggtcccggtg aaggttcccg aaggccattt cttcgccatg
     2221 ggggacaacc gcccgaactc ccaggacagc cggttctggg gattcgtgcc gaagcagaac
     2281 atccggggtc cggtgttttt caggtactgg ccgctgaaca ggatcgggat ggtggactga
     2341 ttgggacgga ccgtctggta tcccggccac atggcgaagg ggaagcggaa gcttgccgaa
     2401 ctcgccgaaa agctggatat cattctggag gtccgggacg cccgggctcc cgtcgtgacg
     2461 tcctctccgc tgtctgagca gctttcccgg atttgccccg tggcggtggt gctgtcgaaa
     2521 aaagaccttg ccgacgggaa ggggacggcg aaatggctct cctggttcac ctcatcgggc
     2581 agaaaggcct gggcggtgaa cctgctcaag ccttcgatgg accagatcag gaaggatctg
     2641 gcccccttcg ggccctccca cagggaagtc cgcctcgcgg tggtgggcat cccgaacgtg
     2701 ggaaaatcaa tgttcctgaa cgcactggtg ggcaagtcct ccgcccaggt ggggggtatt
     2761 cccggaatca ccaggggtgt ttcatggtac aagggaaagg ggtttctggc ggtggattcg
     2821 cccggcatcc tcgacccccg ctccggcgac tccgtccagc ggtgtctcgc atggctcggg
     2881 agcagcaagt ccgaggtgat cggcgggtat gacctcatcg ccctcgacct gatctcggtg
     2941 ctccggaggc ggaacctctg ggtcatggtg gaggagaagt ggggcgttcc cgcaccggaa
     3001 gagctccctg gggaagaaat acttgaagcc ctgggacggc gtctcgggtg ccttgtcgcc
     3061 ggaggggccg tggatattct ctccgcgggc cgcaagttcg tggaggcttt ttccaccggc
     3121 cggctcggcc cggtgaccct cgagctgccg ggggattcgc catggatctg agccggtggg
     3181 gcagaatcgc cggggcggat gaggccggac ggggccccct cgccggcccg gtggtcgccg
     3241 cagcggccgt tctgacccct gcccaggaag aggagctgct ctccctgggg ctcaccgatt
     3301 ccaagaaact caccccccgc agacgggaat tcctttttga acggatgctc ttcctgaagg
     3361 tccggtggcg ggcccaggcc gcctctccgg cgagaatcga cggaatgaac atcctccggg
     3421 cgtctctgtg ggccatgggc cggagcgtgg agaagcttcc gccgtgcttc gacacggtca
     3481 tcgtggacgg cacgtttccc cttccggggc tatcctgtcc ccaggtcccc ctcccgaagg
     3541 ccgacagcct tatccctgcc gcagcagccg catcggtggc cgcaaaagtg ctccgggaca
     3601 ggatcatgga tattcttgac ggcctgtacc ccggctacgg tttcaaaaag aacaagggat
     3661 atcccacggt ggcccacaga aaagctctcg aagccctcgg cccttcggcg gtccacaggg
     3721 ccggcttcac ctggagggct ccctgatggc agcccagggc atcggcggca tctccggccc
     3781 gtcggtccgc cccggccatc ccgaggggca gacccagccc cgggtggacg gaagcccccg
     3841 gcagcccgga acccagcttc cccggatcgc cgacggaacg gtggtgaacg gcctcgtcct
     3901 ggaagccagg gaggagggat cctacctcgt tcgggtggcc gggcaggccc ttcttgcccg
     3961 ggcgaacctt ccccttcttc cgggacagca tttccgggcc gtgtgggacg cctccggcga
     4021 catccccgtc ctccgcctct ccgacgcgga ggcagccctt ctcggaaaac tccccgcagg
     4081 ggaccgggaa gtggcctccg ccctcctctc ccgcggcctt cctctcgaca gccgggccct
     4141 cgcctccctg agaagcgcct ggtccggcat gggcgggcag ccctcgcagc tcgctcccct
     4201 cgcggaactc tgggcccggg gcattcccct cacggcgggc aacgtgcagg tcctcgcatg
     4261 gtatttcacc ctcggcgaaa acgaggcggc ctccctgtgg aaaaaagtga gggatgaaat
     4321 ccgcagccgg gcggccaggg gagaaaatcc gctggccatc ctgaaggaga tgaccggggg
     4381 agacgacgaa cggggcgcct ttctccgggg gcatgccctg ctttccaggc cgtcacggga
     4441 agggatcgac ccgtccctcc ttgcgcccgc ccttcttccg gccggcgacg gggagggaac
     4501 cctcacggca cggatcacca ccggtgcatg gaaacggcag ggaaagtctt tctggtccat
     4561 ttccttcgag atggagggca accgcatcgg ccccgtgggg ggagacgtgg agagcgacgg
     4621 caaaaatctc ggagttaccc tgaaggcgga gcggagcgaa actttcgagg ctctcaggat
     4681 gcggcgtcat cttcttcggc aggagctcgg cagccttccc ctggcgctgc agcatctcgc
     4741 cgtggcccgt gggaggagag tccccaggat tcccggcaga ggcctggaca taacggtatg
     4801 aacgggaagg gaaagaagcg gcttcaggcc gcggcggtcc agtacgacga aaagcatgat
     4861 gccgctccga gggtgacagc ctccgggaag gggtacgtgg cggagaaaat cctcgagctc
     4921 gccagagagg cggacgttcc cgtggtggag gacgccgccc tcgtgagcgc cctgatggtt
     4981 ctggagctcg gcgaggagat ccctgcggag ctttacgagg cagtggcccg gattctcgcc
     5041 ttcatctaca aactcgacaa aggagaaaaa ccatgacggc ggaacatctt gaacggggac
     5101 ggcggggcga ggagatcgcc gcggcctacc ttgccggcct ggggtggaca atactcgacc
     5161 gtaacgtggt cttccgcggc ggggagcttg acctggtggc cctttccggc ggggagctcg
     5221 ttgtggtgga ggtcaggacc cgctccgaag gctggatgca gcggggggag gagtccgttg
     5281 ggccccgg
//
LOCUS       DAOTHD010000024         5284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_18528_length_5284_cov_3.382865, whole genome shotgun sequence.
ACCESSION   DAOTHD010000024 DAOTHD010000000
VERSION     DAOTHD010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18528_length_5284_cov_3.382865"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="P5219_00855"
     CDS             complement(<1..299)
                     /locus_tag="P5219_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25335.1"
                     /translation="MSALFSVPYLEEEPPSVPELRRSPAEKLPVPAETVLSSVEGEER
                     LFPHFTGKAGKPGGGDIGRIADHQVEFFVPDGDVLRIPGMPEEAAGKKAVLFQISP"
     gene            438..1625
                     /gene="trxB"
                     /locus_tag="P5219_00860"
     CDS             438..1625
                     /gene="trxB"
                     /locus_tag="P5219_00860"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRX25336.1"
                     /translation="MEKRELVIIGAGPAGLSAAIYGRRAGLDVLLLEKGVPGGQINIT
                     EEIENWPGVEHATGPELGAMFRSHAEKFKTEFRDVDVSAVEVRDGAKIVVTNKGEIEA
                     EAVIIATGAYFRRLGCEGEAEHIGQGVSYCAVCDGAFFEGEEIAVVGGGNTAVEEGEY
                     LTTFASKVYIIHRRDEFRADRAAIERALSNPKIVPVWNSVVEKIEGDGMVENLVLKNI
                     KTGEVSNLPVAGVFMFVGQSPHDEPVRGLVEAAKGGWIRTNDAMETSVEGIFAAGDVR
                     DKGLRQVVTAASDGAIAAMSASAYINEQVHLRSVLMEPEHVTALFYSSIDREQVKLSD
                     EVESLARNAGKKVALVDGYKNARMVEKLGLPSLPAMVELASGKTVKMAPVADASAAEA
                     FLQ"
     gene            1693..2625
                     /locus_tag="P5219_00865"
     CDS             1693..2625
                     /locus_tag="P5219_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HRX25337.1"
                     /translation="MILTVTLNPAVDEEYVVPEFRPGGWIRATKSSRSPGGKGINVST
                     LLAQFGYTSTAMGFLAGFNGEYIRHALRASRITTNFVHIPGETRTNVYIIDDTGHVET
                     GISEAGPSVTPEVMERLRKVYRRILQRTKMVILGGSLPPGAPEDIFAELVLLAREKGI
                     PTVVDAAGPPLMAALEAGPTVVKADHRFMSKVMGRSLTSLDNLIDVVSGFHEKGVEWA
                     VVSYRTYGNVFFSPDGIYLALADRKSVRSIFSASDALLAGLVVAREEGMTTEDTIRFA
                     MASAWECAAHVGKGIQNRKAVEDYIPLVELEHLS"
     gene            2702..3184
                     /gene="fliS"
                     /locus_tag="P5219_00870"
     CDS             2702..3184
                     /gene="fliS"
                     /locus_tag="P5219_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HRX25338.1"
                     /translation="MDSRNAQNAQAVYIANRVNTASREQLLLITYEIGVKACRNAETA
                     LVSGSTEEANRNIQKAQDVLRELMVTLNMETGGEVAEGLMKLYDFMYLLLVEANVRKE
                     PEKVSVVRGMLEELKGTWEEAVIKLAAEQQEHHLPGVPSADVSRERQAVPAGGLNIAG
                     "
     gene            3174..3635
                     /locus_tag="P5219_00875"
     CDS             3174..3635
                     /locus_tag="P5219_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25339.1"
                     /translation="MQDDFRENLSSLASTELALYNELALLVQKEGECVRSGNLDCLLS
                     ILVEKQDVISRQELVQEGWNTICSGLGLSEGRDGPVFWEKVASLLGPDGTDDLKASLV
                     VIRDVAGSVLEEEQEVQTLLEEHVADLRKEMLRLNRGKKAVHGYYKSGGSF"
     gene            3734..4729
                     /locus_tag="P5219_00880"
     CDS             3734..4729
                     /locus_tag="P5219_00880"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRX25340.1"
                     /translation="MKKEIALGEKVAADVEKQWERVADPALSARLSMLLGRFLPHLSR
                     PLPYEVRIVREKMVNAFSLPGGIVYFTTGMLDFLRTDAEVAAILAHELIHADRRHVMI
                     QTARASKINLAALVLMIASQGSAGPMILTNLAQVAVTNSYGRDLEREADKEGFRILLE
                     AGFPPAAMVTSLEAMIYDQLKRPYVDPGVFMTHPELSERVAYILQTAREKNVPIRRKK
                     ALNLLRPSVESREGKTVLLLDGDPVWSLPESGESRAAAVNAAEKIDDLLQMETAPYEI
                     QIISLDGKNALRVGPSIIAREPLPKGAGTLESFRESLVQSLGEARNKHPGAKYLH"
     gene            4842..>5284
                     /locus_tag="P5219_00885"
     CDS             4842..>5284
                     /locus_tag="P5219_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRX25341.1"
                     /translation="MTISLYRRYRPRTFEEVAGQDMAVDVLKKALQRNQVGHAYLFSG
                     PRGCGKTSLARLLAKALNCENLKDGYEPCGECPGCVSITAGESLDVVEIDGASNNGVE
                     EIRELKSHVSLSPFSSKWKVYIIDEVHMLSISAFNALLKTLEEPPS"
BASE COUNT         1158 a         1470 c         1557 g         1099 t
ORIGIN      
        1 ggagaaatct gaaaaagtac ggccttcttt cccgctgcct cctctggcat tccaggaatc
       61 cggaggacat ccccgtccgg gacgaagaac tcgacctggt ggtcagcaat cctccctata
      121 tcccctcctc caggctttcc agccttcccg gtgaagtgag gaaagagcct ctcttcgccc
      181 tcgacggagg agaggacggt ttctgctggt accgggagct tttccgctgg gctccgtcga
      241 agctcaggca cggagggcgg ctcctcttcg agatagggga cggagaacag ggcggacatc
      301 tggctgaaat tgctccatca tgtcttatat ttaccggaat attccatgat atgtctcgca
      361 aacctcgcgc agtttcgtgg cttcgtgtat aattcccgtg gttactatcg tttttcaact
      421 ggaggggtgg atttctgatg gaaaaaaggg agcttgtcat catcggcgca gggcccgccg
      481 gcctttctgc ggcgatttac ggcagaagag ccggccttga tgtgcttctt cttgaaaagg
      541 gtgttcccgg gggacagatc aatattaccg aggaaataga aaactggccc ggagtggaac
      601 atgccaccgg tcctgaactt ggcgccatgt tcaggagcca tgctgaaaag ttcaagacag
      661 agtttcgtga tgtcgacgtg tccgccgtgg aagtccggga cggagcgaaa attgtcgtga
      721 caaataaagg tgagatagag gcagaggctg tcatcatcgc cacgggagcc tatttccgcc
      781 gcctcgggtg cgaaggggag gccgagcata tcggtcaggg cgtgagctac tgcgccgtct
      841 gtgacggggc ttttttcgag ggcgaggaga ttgccgtcgt cggagggggc aataccgccg
      901 tggaggaggg cgagtatctc accacattcg cttcgaaggt ctacatcatc caccggaggg
      961 acgagttccg tgccgaccgc gcagccatag agcgggccct ctcaaacccg aagatcgttc
     1021 ccgtatggaa ctcggtggtt gaaaagatcg aaggggacgg tatggtggag aacctcgtcc
     1081 tgaagaacat caagaccggc gaggtttcca acctcccggt ggcgggagtg ttcatgttcg
     1141 tcggtcagtc cccccatgac gaacccgtga ggggcctcgt ggaagccgcc aagggcggat
     1201 ggatcaggac caacgacgcc atggaaactt cagtggaagg aatcttcgca gcgggagatg
     1261 tccgcgacaa ggggctccgc caggtggtca ccgccgcatc cgacggtgcg attgccgcca
     1321 tgagcgcgtc agcctacatt aacgagcagg tccatctccg gtccgtcctc atggaaccgg
     1381 agcatgtgac cgcccttttt tactccagca tcgacaggga gcaggtgaag ctctccgatg
     1441 aggttgagtc cctggcgaga aacgccggga aaaaggtcgc cctcgtggac ggctacaaga
     1501 acgcccgcat ggttgagaaa cttggtcttc cctcccttcc cgccatggtg gagcttgcct
     1561 cgggaaaaac ggtgaaaatg gctcccgttg cggacgcctc ggcggccgag gcgtttctgc
     1621 agtagatcaa aacatttcag ggatgttccc ggggcgctcc gggaacatcc tgtgcccggg
     1681 gaggaacagg tcatgattct gacggttacc ctcaatcctg cagtggacga agagtacgta
     1741 gtgcctgaat tcaggcccgg aggatggatt cgggcaacga aaagcagccg ttcccccggc
     1801 ggcaaaggaa tcaatgtttc gaccctcctg gcccaattcg gctatacctc aactgcaatg
     1861 gggtttctcg cggggttcaa cggggaatat atccgtcatg ccctccgggc ttcccgaata
     1921 accacgaatt ttgtccacat tcccggcgag acccgaacca acgtgtatat catcgatgat
     1981 acagggcacg tggaaacagg aatttccgag gcagggccgt cggtcactcc ggaagtcatg
     2041 gagcgcctgc ggaaggtcta ccggaggatt ctgcagagga caaagatggt catcctgggc
     2101 ggttccctgc ctccgggcgc tcccgaggac attttcgcgg aactcgtcct tcttgccagg
     2161 gaaaaaggaa tacccaccgt ggtcgatgcg gccgggcccc ctctcatggc tgcccttgag
     2221 gccggcccca ccgtggtgaa agctgaccac cgcttcatgt caaaagttat ggggcgttct
     2281 ctcacctctc tggacaacct gattgacgtg gtctcgggat ttcatgagaa aggcgtggag
     2341 tgggcggtcg tatcctacag aacctacggc aacgtttttt tctcccctga cggtatctac
     2401 cttgccctgg cggacagaaa aagcgtccgg tcgatttttt cggccagtga cgcccttctc
     2461 gccgggcttg ttgtcgcgag ggaagaagga atgacaacgg aggatacgat ccggtttgcc
     2521 atggcatcgg cctgggagtg cgctgcccat gtgggaaagg gcattcagaa cagaaaagcg
     2581 gtggaagatt atattcccct tgtggagctt gagcacctct cctgaaatct cttttttgcg
     2641 ggcggggtaa tgttttcccc cccgccggcc gaaacagagg gagaggaagg aggaattttt
     2701 tatggactcc aggaacgccc agaatgccca ggcagtatac attgcaaaca gagtgaacac
     2761 cgcttcgagg gagcagcttc tcctcattac atacgaaata ggtgtgaagg cctgcagaaa
     2821 cgccgaaaca gcccttgtct ccggtagcac ggaggaagca aacaggaaca ttcagaaagc
     2881 ccaggacgtt ctccgtgagc ttatggtcac cctcaacatg gagaccggcg gagaagtggc
     2941 cgaaggactg atgaagctgt acgacttcat gtacctcctc ctcgtggaag ccaacgtccg
     3001 caaagagccg gaaaaagtct ccgtcgtgag agggatgctc gaggaactca aggggacatg
     3061 ggaggaagct gtcatcaagc tggccgcgga acagcaggaa catcatcttc cgggcgtccc
     3121 ttcggccgat gtatccaggg agcgccaggc agtgcccgcg ggggggctca acattgcagg
     3181 atgatttccg tgagaacctt tcgagcctgg catctacaga acttgccctg tacaacgaac
     3241 tcgccctcct tgttcagaaa gagggcgagt gtgtcaggtc ggggaatctc gactgtcttc
     3301 tttcaatcct tgtcgaaaag caggatgtga tttcccggca ggaactggtg caggagggat
     3361 ggaacaccat ctgcagcggc cttggccttt ccgagggcag ggacggtccg gtcttctggg
     3421 aaaaagtcgc ttcccttctg ggtcccgacg ggacggatga cctgaaggcc tccctggtgg
     3481 tcatccgtga tgtggcgggc agtgtgctcg aagaggagca ggaagttcag acccttcttg
     3541 aagagcacgt ggcagacctg agaaaggaaa tgctcaggct gaaccgggga aagaaggccg
     3601 tccacggcta ttacaagtca ggagggtcgt tctagaggaa ttgcccatga aattttgcag
     3661 gagaaccgcc ggcgttctgt ttttttgcat gttctttctg accgctcttc ccctccgcgc
     3721 ttctcaggac actatgaaaa aggaaatcgc tctcggcgag aaggtcgcag ccgatgtgga
     3781 aaagcagtgg gaacgggtag ccgatcccgc cctgagcgca cggctttcca tgcttctcgg
     3841 ccgctttctt ccccaccttt cgcgccctct cccctacgaa gtcaggatcg tacgggagaa
     3901 gatggtcaac gccttcagcc ttccgggggg tatcgtttac ttcaccacgg gcatgcttga
     3961 tttcctccgt accgatgcgg aggtggcggc cattctcgcc catgagctca ttcatgcaga
     4021 caggcgtcat gtcatgatcc agacggcccg ggcatcgaag atcaatctgg ccgccctcgt
     4081 gctgatgata gcttcccagg gaagcgcagg gcccatgatt ctcacaaacc tcgcccaggt
     4141 agcggtgacc aattcctacg gcagggatct tgaacgggag gccgacaagg aaggcttccg
     4201 catcctcctg gaggccggct tccctcctgc ggcgatggta acctcccttg aagccatgat
     4261 ctatgaccag ctcaagcgtc cctacgtgga cccgggcgtt ttcatgaccc atcccgaatt
     4321 atctgaacgg gtggcttaca tcctccagac agccagggag aaaaacgttc ccatccggag
     4381 aaaaaaagcc ctcaatcttc tccgtccgtc agtggaatcc agagagggaa aaacggtttt
     4441 gcttctggac ggcgatcctg tctggtcgct cccggaaagc ggcgaaagcc gcgccgccgc
     4501 tgtaaacgcc gcggaaaaaa tcgacgacct gctccagatg gaaaccgcac catacgaaat
     4561 ccagatcatt tcccttgacg ggaagaatgc tctccgcgtg ggtccttcga ttatcgccag
     4621 ggaacctttg cccaaaggcg ccggaactct cgaatccttc cgggaatccc tggtgcagtc
     4681 ccttggggaa gcccgaaaca aacaccccgg agcgaaatat ctccactgat tttgatgtag
     4741 aatagcatgg gtttccgtag ccgcccggcg ttttccccgg tcggcaatgt ttctgcttcg
     4801 gctgcagccc catgattttc ccggatagag gaggacgccc gatgacgatt tcgctttaca
     4861 gacggtacag gccccggacc tttgaggagg ttgccgggca ggacatggcc gtggacgtcc
     4921 tgaaaaaggc cctccagcga aaccaggtgg ggcacgccta tcttttttcc ggtcccaggg
     4981 gatgcggcaa gacttctctt gcgaggctgc tcgccaaggc cctgaactgt gaaaacctga
     5041 aggacggcta cgagccctgc ggcgagtgcc cgggctgtgt ctccattacc gctggagaaa
     5101 gccttgacgt tgtcgaaatc gacggcgctt cgaacaacgg ggtcgaggaa atccgggaac
     5161 tgaaaagtca cgtctccctt tcgccctttt cctccaaatg gaaagtgtac atcattgatg
     5221 aagtgcacat gctctccatc tcggccttca atgctctgct caagaccctg gaggagcctc
     5281 cttc
//
LOCUS       DAOTHD010000025         5229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_18774_length_5229_cov_4.123695, whole genome shotgun sequence.
ACCESSION   DAOTHD010000025 DAOTHD010000000
VERSION     DAOTHD010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18774_length_5229_cov_4.123695"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..864)
                     /gene="panB"
                     /locus_tag="P5219_00890"
     CDS             complement(10..864)
                     /gene="panB"
                     /locus_tag="P5219_00890"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRX25342.1"
                     /translation="MAKKKGRLDFVNMKKNGEQVTWVTAYDFPMASFAEAAGMDMILV
                     GDSLGMITLGYQGTIPVTMEDCISHCKAVRRGAPNTFVMGDMPFGSYQVSDQQAVENA
                     VRFFKEADMDAIKLEGGVRVESRIKAMADAGMLVCGHIGLTPQSSGPLGGFKAQGLDP
                     ASARYVIEDALAVERAGAYALLVEAVPPELTQFLAKKLTIPVYSIGAGAPCDGQLLIC
                     GDMIGMFQAFTPKFVKVYANVAEVITNAFKEYVEDVRTGKFPGDEHCYHVRKGMEEEY
                     AAMLKEYE"
     gene            complement(1026..1394)
                     /locus_tag="P5219_00895"
     CDS             complement(1026..1394)
                     /locus_tag="P5219_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25343.1"
                     /translation="MRQDRKKKVKITKPRQKCRGLENPTFFGAWIQFFSNEEVLRKNK
                     VDKVFTFLYPVLPFYRTKISFFPGGVNDCPPLITEKEKKRNIILNPLFLPVGFRSPSF
                     CKKPLAKDIRGGDSLSARKA"
     gene            1393..2223
                     /locus_tag="P5219_00900"
     CDS             1393..2223
                     /locus_tag="P5219_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRX25344.1"
                     /translation="MPPLGRGEAEVPPSYTLAAYPTNDPKKVYDALKPLADHIAAETG
                     WTVRMVVTRNYEEIIHRLTDGTVDIAMLSAASYVRFGPSVPGLSYVATYMEMEGTAPS
                     PFYHSVILCLADSSVTDISGLKGKNFAFTDRYSTSGYIVPLLLFRDRGIVPDSFFGKT
                     FFVGKHDAVIEALKARSVEGGAVSSGTLANAEAVEGNIFRVLASSEPIPLDAIAAGKR
                     VPQEHVKLLQEALRRLPPDSAPVKAVRVHLQWPAAGFAVLGDSFYDPLRRALGISSGD
                     "
     gene            2244..3722
                     /locus_tag="P5219_00905"
     CDS             2244..3722
                     /locus_tag="P5219_00905"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRX25345.1"
                     /translation="MKLYWKVLITAMLPLFLVLGLGMYQVNRSVRIHEESVAEENRLK
                     VSLLRFRLSSFLKEMQKTASILGASGDAARAVEGADTDYLTAWGKLFLDGGGTYRILF
                     TDLDGTVLSRPHDPFRFGDSLRSHPAVRSAAEGRDVAAFYEIEGFFSIVCAAPVKLYG
                     EIPVGTVMVAAPVSPALLASFVSGTGLDLLVEPEGYPPAASFDGTCGRSFLSLDPELV
                     HEGIPMKRVLVFFPDDLAITPLTELHSNLVIAVGFFFIALPLGLFLMLRRHLRPYARL
                     MENLLHLSDKDADLPGVRERLEAEDGNARNEVSAVTAAVARILKMLEEKIALLEWTSR
                     TDALTQVNNRLHLDRVLQREIGCIEREDPPLSVLILDLDHFKDINDTFGHQAGDGVLR
                     KTAGILSSVVGGRGSVGRWGGEEFLVILPGTGLDNAFLLAEEIRCEVERGDFGITRYV
                     TVSIGVAQAVAGDTPVSLVRRADVGLYRAKAGGRNRTVLGER"
     gene            3875..>5229
                     /locus_tag="P5219_00910"
     CDS             3875..>5229
                     /locus_tag="P5219_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HRX25346.1"
                     /translation="MVRLRILKTYFSEILLLLAATVIITILVLSWFLNYHFEQFSASM
                     ASRLNREFLAENRRMNEYLQKMIRISGMELFLEPSIQNLMYRGDLKNFDEVTGIRRLD
                     AVMSAGMHIHSIYVYNGARKYVYATSNVDSDRADRFRDQGALSLLGGAVDRRRLEPIP
                     RFSLQASGEVPVYSFVFYDIRKTEPRIGGALIMNITLDWLREVFLDTPSSPSRVMFVD
                     ADGTIAYHSDPSMFLKNIADDGLFRRLTDRGDRSGSFSFLSGEDEVFVFYSRSGENSP
                     FLIRTYPYGAIMGEILARRRTTMILVLVCVPLAMLLVVAVSRKLYRPIRQLVRRVDPH
                     AEGETSGQGDLGFLSSRIDSILSRAETVEETAESYRKLLQTDVLRGALSGKTADPGSL
                     LEQFREFRIPFSPEKAFGLAGLRPFTDEQLDRLMSHFPETALMGVSFDGERMVVLFQV
                     SS"
BASE COUNT          951 a         1611 c         1578 g         1089 t
ORIGIN      
        1 atgtcctctc tactcgtatt ccttcagcat ggcggcgtac tcctcctcca tgcccttcct
       61 cacgtggtag cagtgctcgt ctccggggaa cttgcccgtc cgcacgtcct ccacgtactc
      121 cttgaaggcg ttggtgatga cttccgccac gttggcgtag accttcacga atttcggggt
      181 gaaggcctgg aacatgccga tcatgtcgcc gcagatcagg agctgcccgt cgcagggggc
      241 accggcgccg atggaataca cgggaatggt cagctttttc gccaggaact gggtgagctc
      301 aggcggtacg gcctccacga gcagggcata ggctcctgcc cgctccaccg caagggcgtc
      361 ctcgatgacg taccgggccg atgcgggatc gagtccctgg gccttgaacc ctccgagagg
      421 gccggagctc tggggggtga ggccgatatg gccgcacacc agcatccccg cgtcggccat
      481 ggccttgatc cgggactcca cccggacgcc gccctcgagc ttgatggcgt ccatgtccgc
      541 ttccttgaag aagcgcacgg cgttctccac ggcctgctgg tcggagacct ggtaggaacc
      601 gaagggcatg tcacccatga cgaaggtgtt cggcgcgccg cggcgcacgg ccttgcagtg
      661 gctgatgcag tcttccatgg tcacggggat ggtcccctgg taccccaggg tgatcatgcc
      721 cagggagtcc cccacgagga tcatgtccat ccccgcggcc tcggcgaagc tcgccatggg
      781 gaagtcgtag gccgtgaccc acgtgacctg ttctccgttc ttcttcatgt tcacaaaatc
      841 gaggcgccct ttcttctttg ccatcgctca ttctcctttc cccgtcgagg ggtgtgaatt
      901 tagaagtccc ggacggcgtt caccgtttcc aggaacgaat ccgcactgag accgcaggct
      961 gtgacgactg cagaggctcc ggcacaggaa aaggtattcc gggacccgcg ggcacaatca
     1021 ctattttatg ctttccgggc tgaaagggaa tccccccccc ggatgtcttt tgccaaaggt
     1081 tttttgcaga atgacgggga acggaaaccg accggcaaga ataagggatt caatattata
     1141 ttccgttttt tctctttttc cgtgatgagc ggagggcagt cattgacgcc accggggaag
     1201 aatgatattt ttgtcctgta aaatggaaga acaggatata agaaggtgaa tactttgtcg
     1261 actttattct tccggagaac ctcttcatta gaaaagaact gtatccaagc tccgaaaaaa
     1321 gtcgggtttt ctaagccccg gcatttctgc cggggcttgg tgatttttac ctttttcttc
     1381 ctgtcctgtc tcatgccccc tctcgggcga ggagaggccg aggtgcctcc ttcctacacc
     1441 cttgcggcct atcccaccaa cgacccgaaa aaagtctacg acgccctgaa gcccctggcg
     1501 gaccatattg ccgccgaaac ggggtggacg gtccgcatgg tggtcaccag aaactacgaa
     1561 gagatcatcc accgtctcac cgacggtaca gtggacatcg ccatgctcag cgcggcgagc
     1621 tatgtccgct tcggaccctc cgttcccggt ctttcctacg tggccaccta catggaaatg
     1681 gaggggacgg cgccgtcgcc cttctatcat tcggtgattc tgtgcctggc ggacagttcc
     1741 gtcaccgata tttcgggcct gaaggggaaa aatttcgcct tcaccgaccg ctattccacc
     1801 tccggctaca tcgttcccct gcttctgttc cgggacaggg ggatcgtccc tgattccttc
     1861 ttcggcaaaa ccttcttcgt cggaaagcat gatgccgtca tcgaagcgct gaaagcccgc
     1921 tccgtggaag ggggcgccgt atcctccgga acccttgcca acgccgaagc cgtggagggc
     1981 aatattttcc gggtgcttgc ctcttcggag cccatccctc tcgatgccat agcggccggg
     2041 aagagggtgc cccaggagca tgtgaagctt ctgcaggaag cgctccgccg tctgccgcct
     2101 gattccgctc ccgtgaaggc cgtccgggtt cacctgcagt ggcctgccgc cggattcgcc
     2161 gtgctgggcg actcctttta cgatcccctc cgccgggctc tcgggataag ctccggcgac
     2221 tgaggacgaa ggggggaact ggtttgaagc tgtactggaa agttctcatc acggccatgc
     2281 tgcccctgtt cctggtcctc ggcctgggca tgtaccaggt gaaccgttcg gtcaggatcc
     2341 atgaagagag cgtggcggaa gagaaccgcc tgaaggtgtc cctcctccgc ttccggcttt
     2401 cctcttttct gaaggaaatg cagaaaacgg catccattct cggcgcctcg ggagacgctg
     2461 cccgggccgt tgagggcgcc gataccgact acctgaccgc ctggggaaag ctcttcctcg
     2521 acgggggcgg aacgtacagg attctcttca ccgacctgga cggcacggtg ctctccaggc
     2581 cccatgatcc tttcaggttc ggcgacagtc tccggtccca cccggcggtt cggagtgccg
     2641 ccgaaggaag ggacgtggca gccttctacg agatcgaggg gtttttcagc atcgtctgcg
     2701 cggctcctgt gaaactttac ggcgaaattc ccgtgggaac ggtcatggtg gcggcgccag
     2761 tttccccggc gctccttgcc tcctttgtct ccggaactgg tctcgacctg cttgtggaac
     2821 cggaggggta tccgcctgca gccagctttg acgggacctg cggcagatcc ttcctttccc
     2881 tggacccgga acttgtccac gagggaatcc ccatgaaaag ggttcttgtt tttttccccg
     2941 acgacctggc cataaccccc ctcacggagc tgcacagcaa cctggtaatc gcggtgggat
     3001 ttttcttcat cgccctcccc ctggggctct tcctgatgct ccgccgtcac ctccgtccct
     3061 acgcccgtct catggagaac cttctgcacc tttccgacaa ggatgcggac ctgccgggag
     3121 tcagggagag gctcgaggcg gaggacggaa acgcccgcaa cgaggtgtcc gcggtgacgg
     3181 cggcggtggc gcgaatcctg aagatgctgg aggaaaagat tgcccttctc gagtggacct
     3241 cgaggacgga tgccctcacc caggtgaaca acaggcttca cctcgacagg gtgctccaga
     3301 gggagatcgg gtgcattgaa agggaagatc ctcccctgtc tgtgcttatc ctggacctgg
     3361 accatttcaa ggatataaac gacaccttcg gccaccaggc cggtgacggg gtgctccgca
     3421 agacggcggg catcctctct tcggtcgtgg ggggacgggg ttccgtcggc cggtgggggg
     3481 gcgaggagtt cctggtcatt cttcccggaa cgggcctgga caacgccttt ctcctggccg
     3541 aagaaatccg gtgcgaagtg gagcggggcg atttcggcat cacccggtac gtgacggtga
     3601 gcatcggcgt cgctcaggcc gtggccgggg acacgccggt gtcccttgtc cggagggccg
     3661 acgtcggcct gtaccgggcc aaggcgggcg ggcggaacag gacggttttg ggagagagat
     3721 aggctcaaaa ttaatggaaa cactctccgg gccggcggga tgaagaacat cctgtccacc
     3781 ggccctgaag acggagcggc gtatggtacc atattttcgg gtgcatccgc acagagattc
     3841 gccgccgcag gaggaaggag ccgctccggg agccatggtc agactcagga tactgaaaac
     3901 ctatttttcc gagatcctcc tgctgctggc agctacggtc atcatcacca ttctggtgct
     3961 ctcctggttc ctcaactacc atttcgagca gttctccgcg tccatggcga gcaggctgaa
     4021 cagggagttc ctcgcggaaa accgccggat gaacgaatac ctccagaaaa tgatccgcat
     4081 cagcggcatg gagctgttcc tggagccgtc gatccagaac ctcatgtacc gtggggacct
     4141 gaaaaacttc gacgaagtga cggggatacg gcgtctcgac gccgtcatga gcgcggggat
     4201 gcacattcat tccatctatg tctacaacgg cgcgaggaag tacgtgtacg ccaccagcaa
     4261 cgtggattcc gacagggcgg accgcttccg ggaccagggg gctctctccc tgctcggcgg
     4321 ggccgtcgac cgcaggaggc tcgagcccat tccccgcttt tctctccagg cttccggcga
     4381 ggtacccgtc tattccttcg ttttttacga catccggaag acggagccga gaatcggcgg
     4441 ggccctcatc atgaacatca ccctggactg gctccgggag gtctttctgg acacgccctc
     4501 ctccccgtcg cgggtgatgt tcgtggacgc tgacggcacc atagcgtacc attccgaccc
     4561 gtccatgttc ctgaagaaca tcgccgatga cgggctcttc cggcggctga cggaccgggg
     4621 agaccggagc ggctcctttt ccttcctgtc cggggaggac gaggtgttcg tcttctattc
     4681 caggagcgga gaaaattccc ccttcctcat ccggacctat ccctacggcg ccatcatggg
     4741 ggaaattctc gcccggcggc ggaccaccat gatcctggtg ctggtctgcg ttccccttgc
     4801 catgctcctc gtggtggcgg tgtccaggaa gctctacagg cccatcaggc agctcgtgcg
     4861 cagggtggac ccccacgcgg agggggaaac gtccggccag ggagatctcg ggttcctctc
     4921 ctcccggata gacagcatcc tgtccagggc cgagacggtg gaggagacgg ccgagtccta
     4981 ccggaagctc ctgcagaccg acgtgctgcg gggggccctg tcgggaaaga ccgccgatcc
     5041 cgggagcctt ctggagcagt tcagggaatt ccggattccc ttctctccgg aaaaggcctt
     5101 cggcctggcg gggctcaggc ccttcacgga cgagcagctg gaccggctca tgtcccattt
     5161 tcccgagacg gccctgatgg gggtgtcctt cgatggggag cggatggtgg tcctgttcca
     5221 ggtaagctc
//
LOCUS       DAOTHD010000026         5184 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_18976_length_5184_cov_2.548840, whole genome shotgun sequence.
ACCESSION   DAOTHD010000026 DAOTHD010000000
VERSION     DAOTHD010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18976_length_5184_cov_2.548840"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="P5219_00915"
     CDS             complement(<1..351)
                     /locus_tag="P5219_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRX25347.1"
                     /translation="MRYLPFGSTGAIVSELGFGGIPIIRLTTEDAVRVLKRAYDRGIN
                     FFDTANSYEGSEEKFGSALSGVREKIFIATKSKKRDGKGVTEHIELSLKRMKTDVLDL
                     VQIHGSSYTPALAAS"
     gene            complement(525..1751)
                     /gene="rocD"
                     /locus_tag="P5219_00920"
     CDS             complement(525..1751)
                     /gene="rocD"
                     /locus_tag="P5219_00920"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HRX25348.1"
                     /translation="MMTRYTSDELMKMEHEYGAHNYHPLEVVISKAEGIWVEDPEGRR
                     YMDMLSAYSAVNQGHRHPKIIKALKDQADILTLTSRAFFNDKWPIFAKKLSGLTGKEM
                     ILPMNTGAEAVETAIKTMRKWGYMVKGVEKDKAEIIVFADNFHGRTVTIISFSVDPDA
                     KENYGPFTPGFVVVPYDNLEAVEKAVNKNTVGILVEPIQGEAGVVVPTEGFLKGLESI
                     ARKNNLLLAVDEIQTGFCRTGKTFAFEHEGISPDIIIMGKALGGGVMPVSAIAADKDV
                     LGVFLPGTHGSTFGGNPLACAVASASIDVLVEEKLAERAAELGAYFMDGIRALNSPKI
                     RLVRGKGLLIGVVLHESAGKARKYTTALKEKGLLCKETHNWIIRFAPPLVITKQEIDT
                     ALGKMAEVFCGVEAKA"
     gene            2077..3042
                     /locus_tag="P5219_00925"
     CDS             2077..3042
                     /locus_tag="P5219_00925"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRX25349.1"
                     /translation="MRKMSRILMFSFLFVFLASAAFAAFPERSVSVVCPWGAGGGTDR
                     LTRFMASQLEKELGKPFPVTNKTGGNGAVGHSAGAFSKPDGYTITMVTLELATMHWMG
                     LTELTYADFDYVIQLNQDPAGVMVKADAPWKNLGELLEAVKATPEKFKFSGTAAGGVW
                     DLARIGMLDKAGIPVNAVSWIPTTGAAPSIIELLGGHVEVITCSLPEAASQIAAGEIR
                     PLAIMADIRDSAFPDVPTLKESGIDWSAGTWRGFAVPKGTPAEVIDVLYNAMKKITDS
                     DEYKEFMSKNGFGVLVRGPKEFAEFARQQDTDLKNVMELGGYLKK"
     gene            3108..3572
                     /locus_tag="P5219_00930"
     CDS             3108..3572
                     /locus_tag="P5219_00930"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRX25350.1"
                     /translation="MKKTLRILFGVSLVLVSLWVIRTSSTFPEAVSAGKKIPGPAFFP
                     VLLSVVLIPCGFHQAVSALRSGTEAAGRRWGSGTVNGAIVILSLVVYVQLMQWMGYAL
                     STFLFSMFLLLRLRVGVLKALAVTIITVVFILLVFGRLFQIQLPLGELGIPW"
     gene            3585..5066
                     /locus_tag="P5219_00935"
     CDS             3585..5066
                     /locus_tag="P5219_00935"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRX25351.1"
                     /translation="MFEEILAVFRPAILFPWLLSMAFGVLIGGTPGLTATMGVALIIP
                     VTYYMEPLAGMAMILGVSFTSIFTGDIPATLLRIPGTPASGAATLDGYELTKQGRGME
                     ALSLDLLCSAIGGFIGILLLMAVAPPLAKFALKFTNFQYFWLGIFGLSMSAVLSAGRP
                     INGVLSATLGLLVSTVGMDITTGLPRFTGGNMELLEGIGFIPAMVGLFGLSEVLKNVG
                     DAKLLGKSSIPEFGRISLLGTLKIILRNKMTVLKASLLGTCVGALPGAGADIAAWVAY
                     GMEKKTSKNPEEFGKGSIKGVIAPTSANNAALGGTWIPALVFGVPGDSITAIVLGAML
                     MYGLRPGPLIFQENLKLVHGIFAVGLISQFLLIPVGLIGIRIFGKILRMPRNIVMVIV
                     LIFSVIGSYAIRNSFFDIYIMAAFGVAGFFFEYLDIPLPPLILAIILGPMIEDNLRVG
                     LIKTHGGFGEFIFDPICLTLIFLILFVFFGGPVMKFFRRNKRK"
BASE COUNT         1041 a         1421 c         1382 g         1340 t
ORIGIN      
        1 cgacgcagcc aatgccggcg tataggagga accgtggatc tgcaccagat cgagaacatc
       61 ggttttcatc cgcttcaggc tgagctcaat atgctctgtc acgccttttc cgtcccgttt
      121 tttgctcttc gtggcgataa agattttctc ccgcacgccg gacagtgccg atccgaattt
      181 ctcttcgctt ccctcatagg aattcgctgt gtcgaaaaag ttgattcccc tgtcataggc
      241 ccttttgagg acccgtaccg catcctccgt agtgagcctg attatgggaa tgcctccgaa
      301 accgagttct gatacgatgg cgcctgtgct gccgaatgga aggtagcgca cgattaccag
      361 ctccttcggg aataacaaga ttttcttaag gataccagag attccccttt tagtgagcag
      421 cctgattacg tcatcaggaa tccacatcag gtacagatga aaaaggctcc tcctgtgaca
      481 caggaggagc ctttttcatc atctttttcc gtattaaggt ttttctaggc tttcgcctct
      541 acgccgcaga agacctccgc catcttcccg agggcggtgt caatctcctg ttttgttata
      601 accagtgggg gagcgaaccg gatgatccag ttgtgggtct ccttgcagag aaggcctttt
      661 tccttgaggg cggtggtata ttttctcgct tttccggccg attcgtggag cacgacgccg
      721 ataaggagcc ctttccccct gaccagcctg atcttggggg agttcagcgc cctgatgcca
      781 tccatgaagt aggccccaag ttccgcagcg cgttcagcca gcttctcctc gacaaggacg
      841 tcgatcgaag cggacgccac ggcgcaggca agggggttcc ctccgaaggt cgagccgtgg
      901 gttccgggaa gaaacactcc cagcacgtcc ttgtcggccg ctatggctga gacgggcatc
      961 accccgccgc cgagagcctt gcccataatg atgatgtccg gcgagattcc ttcgtgttcg
     1021 aaggcaaagg ttttgccagt gcggcagaac cccgtctgaa tctcgtcaac cgcaaggagc
     1081 aggttgtttt tccgggcgat ggattcaagt cccttgagga atccttcggt cggcacgacc
     1141 acgccggctt ctccctggat gggttccacg agaattccga ccgtattttt atttacggcc
     1201 ttttcgacag cctcaaggtt gtcgtaggga acgacgacga atccgggggt gaagggaccg
     1261 taattttcct tcgcatcggg atcgacggag aaggatatta tggtgactgt tctgccgtgg
     1321 aaattgtcgg cgaaaacgat gatttccgct ttgtcctttt cgactccctt gaccatgtaa
     1381 ccccatttcc tcatggtctt gatggctgtc tcgacggctt ccgcaccggt gttcatgggg
     1441 aggatcattt ctttcccggt aagccctgaa agcttcttcg cgaagatggg ccatttgtcg
     1501 ttgaagaatg ccctcgaagt gagcgtgaga atatcggcct ggtccttgag tgccttgatg
     1561 atcttcggat gccggtgccc ctggttcact gccgaatatg ccgagagcat gtccatataa
     1621 cggcgccctt ccgggtcttc gacccagatg ccttcagcct tggaaattac gacttccagc
     1681 ggatggtagt tgtgagcgcc gtattcatgc tccattttca tcagttcgtc tgatgtgtaa
     1741 cgagtcatca tgtcacctct ttccaagatt tcgattcttc gctcctgaag ttcattcgat
     1801 ttgtttttta tggaaagcag ttcattacag gaacgaaaag aatccagtac tattctcgcc
     1861 caaggagcct tttttttcaa gttttggaat cagggccgaa agtacagtat tctcaggtgg
     1921 aaagaacggc gaaagtggtg tagcggatgt cgttttttga tatactgatc aaaaagggag
     1981 tattgcgcat tttgtatccc ttttgaaagg agggtgccgt tttcgggatc agtagttttt
     2041 tgtgtgtcct atttttctga agggagttgg ttttgcatga gaaagatgtc ccggatactg
     2101 atgttttcgt tccttttcgt ttttctggcc tcggctgcct ttgccgcctt ccctgaaagg
     2161 agcgtatccg tcgtctgccc ctggggagcg ggcggcggaa cggacaggct gacccgtttc
     2221 atggcctccc agctcgaaaa ggagctcggc aagcctttcc ccgtcacgaa caagacaggc
     2281 ggcaacggcg ctgtcggcca ttcggcagga gccttttcaa aaccggacgg ttatacgatc
     2341 accatggtga ccctggaact ggccaccatg cactggatgg gcctgacgga gcttacctac
     2401 gcggatttcg actacgtgat ccagctgaac caggaccccg ccggggtgat ggtgaaggct
     2461 gacgctccct ggaaaaacct cggtgagctt cttgaagcgg tgaaggcaac tcccgagaag
     2521 ttcaagttct ccggaactgc tgcaggcgga gtctgggacc ttgcgagaat aggaatgctc
     2581 gacaaggcgg gcatccccgt caatgcggtc agctggatcc ccacgacggg agcggccccc
     2641 tcgatcatcg agcttctcgg aggtcacgtg gaggtcatca cctgcagcct tcccgaagcg
     2701 gcttcccaga ttgcggccgg ggaaatacgg cccctggcca ttatggccga catacgggac
     2761 tccgccttcc cggatgttcc gacactcaaa gaaagcggca tcgactggtc ggccggaacg
     2821 tggcggggct ttgccgtccc gaagggcacg cctgccgaag taatcgatgt tctgtacaat
     2881 gccatgaaaa agattaccga ttccgatgaa tacaaggagt tcatgagcaa gaacggcttc
     2941 ggtgtccttg tccgcggtcc gaaggagttt gccgaattcg cccgtcagca ggacactgac
     3001 ctgaaaaacg tcatggagct gggcggatat cttaaaaaat agtaccttta caagggaaag
     3061 caccccgccg cattccggcg gggtgccctt tcttccggag gctgtcaatg aaaaaaactc
     3121 tgagaatact cttcggcgta tctttggtcc ttgtttccct gtgggtcatc cgtaccagtt
     3181 ccacctttcc cgaggcagtt tcggccggaa agaaaatccc cggcccggct ttttttccgg
     3241 tgcttctttc cgtggtgctg atcccgtgcg ggtttcacca ggcggtttcg gccctgaggt
     3301 ccgggacgga agccgccggc aggagatggg gttccggaac ggtcaacggg gcgatcgtca
     3361 ttctgtccct tgtcgtgtat gttcagctga tgcagtggat gggatatgcc ctttccacct
     3421 ttcttttttc catgttcctt cttctccgtc tcagggtagg cgtgctgaaa gctcttgcgg
     3481 tgacaattat tacggtggtt tttattcttc ttgtcttcgg ccgtcttttt cagatacagc
     3541 tccctctcgg agagctggga attccatggt aggaggggcc tgaaatgttc gaggaaatac
     3601 tcgcggtttt ccgccccgcc atactctttc catggctcct ttccatggcc ttcggcgtcc
     3661 ttataggagg cacccccggc ctgacggcca ccatgggtgt ggccctcatc ataccggtaa
     3721 cctattacat ggaacctctc gcaggaatgg ccatgattct cggcgtgtcc ttcacgtcca
     3781 tattcaccgg ggacataccg gcaaccctgc ttcgaattcc cggaacccct gcttcaggag
     3841 cggccacact tgacggctac gaactgacca aacagggccg tggcatggaa gccctctccc
     3901 tggatctgct ctgttccgcc ataggaggtt tcattggtat tcttcttctc atggcggtcg
     3961 ctcctccgct ggcaaaattt gcccttaaat tcaccaattt ccagtatttc tggctcggaa
     4021 ttttcgggct gagcatgagc gccgttctga gcgcaggcag gcccatcaac ggcgttttgt
     4081 cggcaacgct ggggcttctc gtttccaccg tcggaatgga tatcaccacg ggacttccgc
     4141 ggtttacggg agggaacatg gaactgctgg aaggaatcgg gttcattcct gccatggtgg
     4201 ggcttttcgg tctctccgag gtactgaaga acgtaggaga cgcaaagctc ctcggaaaaa
     4261 gcagcattcc tgaattcgga cgcatctccc ttttgggaac cctgaagatc attctcagaa
     4321 acaaaatgac cgttttgaag gcctcgctcc tgggcacgtg cgtcggcgcc cttcccggcg
     4381 ccggagcgga cattgcggcc tgggtggcct acggcatgga gaaaaagaca tcaaaaaatc
     4441 cggaggaatt cgggaaggga tccataaagg gagtcattgc ccctacgagc gccaacaacg
     4501 cggccctggg aggaacgtgg attccggccc ttgttttcgg cgtgcccgga gattccatta
     4561 cggccatcgt cctcggagcc atgctcatgt acgggctgag gcctggaccg ctcattttcc
     4621 aggagaacct gaagctcgtt cacggtattt tcgcggtcgg actgatctcc cagttccttc
     4681 tcattccggt gggcctcatt ggaatccgta ttttcggaaa gatcctgaga atgccccgca
     4741 atatcgtcat ggtcatcgtg ctcatttttt cggtcatcgg ctcctacgcc atccgaaaca
     4801 gtttcttcga catctacatc atggcggctt tcggcgttgc cggtttcttc tttgaatacc
     4861 tcgatattcc cctccctcct ctcattctcg ccatcatcct ggggccgatg atagaggaca
     4921 atctgagggt cgggctcatc aaaacccacg gaggtttcgg cgaattcatc ttcgacccga
     4981 tctgcctcac cctgattttc ctcatccttt ttgtcttttt cggaggacct gtcatgaagt
     5041 tcttcaggag gaacaaaagg aagtagcttt tctttcccgt tcatagtatg tgtccacaaa
     5101 agtggcaacc ccaaaaaacg ctctgctgcc caagattccc ttcggggaat ctttttttct
     5161 atttcgtctc ttctttgcat tatt
//
LOCUS       DAOTHD010000027         4958 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_20194_length_4958_cov_3.142770, whole genome shotgun sequence.
ACCESSION   DAOTHD010000027 DAOTHD010000000
VERSION     DAOTHD010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20194_length_4958_cov_3.142770"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="P5219_00940"
     CDS             <1..1291
                     /locus_tag="P5219_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRX25352.1"
                     /translation="LWPFSTLGWPDGTEDLDFYYPTSLMVTGFDIIFFWVARMIMMGL
                     EFMDEVPFRDVYIHALIRDEKGQKMSKSSGNVIDPLDMIDKYGADALRMTLAALTVQG
                     RDILLSTGKIETYRLFMNKIWNASRFALMNLEDGVRLSELPAGSPLALHDRWILARTK
                     IVCGEVSRLLDSFGTGEAARLLYDFVWGDLCDWYLELSKPALRGDEGPERKQATQTVI
                     SGVFRDVLLLLHPFIPFVTEELWETFGFGGGFIEQNAWPRWEDRPEYPGAAEKMEFFQ
                     DTVRILRNLRAESRIAPQAFANQAFVQTEENSLLAEVLRENGTIMETLTKIRKITLLS
                     PKDEKPAGSLSSQMESGEVSLVVGDILDIGAEIERLTQEMAAIRKNIEAGRRKLANED
                     FVSRAPAEVVEKEKDRLAENEARLSRIEENLSSLRRS"
     gene            1299..2627
                     /locus_tag="P5219_00945"
     CDS             1299..2627
                     /locus_tag="P5219_00945"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HRX25353.1"
                     /translation="MNRKPRSFEELENVFSSLASAGIRPGLDRISRLMEKLGNPEKDF
                     PAVHIVGTNGKGSTAAITDSILRESGYRTALYTSPHLESPDERLLIDGSPLSLGEWTM
                     AADDIEKALEADVILRADPPTFFELVTAAAFLLCSRNRVEIAVVEAGLGGRLDATNLL
                     SSVVLTFVTSISMDHMDFLGDTIEKIAGEKFAVMREGVPAFFSGRPSGLVPLFRKKAR
                     DSGAFPHVLPEEIRVENVSVSEEGTAFSLLCGGERYQIRTPLHGSFQVENCSLAVAGM
                     LGISGRFHRISEEALLRGTEKARWPGRFEILRADPPLVLDGGHNPDGIMRLVESLRAI
                     YGEKKPTVVFACMKDKDFPAGLSLLLNGAGRLICTSVPGNPRSADPYVLAQAAKNAGW
                     KEDAVEVVPDPLDALKASERTRQGTVCCGSLYFIGWMRTLIFSRREELHF"
     gene            2630..3385
                     /locus_tag="P5219_00950"
     CDS             2630..3385
                     /locus_tag="P5219_00950"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HRX25354.1"
                     /translation="MNGPAYKIECSSVNDSIVFLPPGDEKRFSIRLFLKGAAMNRASG
                     VPGRSRELLLGAFPRKGPLVAPQQVHGTDVIAASTGATLPARPSADGVCVDRSGIEAS
                     LRFADCFPVVIASLSPSPRIILLHSGYKGTVQNIAGRAARSLLGEKGAEPSETWAWIG
                     PGIGKEHYFRKKGEPWTKRGIESFSRHCVSEDGNDVFFDLGGEIRRQLKGAGIVDEMI
                     CSVPLCTFRDNDVCYSYRKGDRENRLFLLAWME"
     gene            3473..4447
                     /locus_tag="P5219_00955"
     CDS             3473..4447
                     /locus_tag="P5219_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRX25355.1"
                     /translation="MDLKRLGVIGVGHLGQHHARVYTELLGTQLVGVVDQNESRAAAI
                     GDNLGVPWFTDMDEFIARARPDAVSVVVPTSLHFEIAKKALSNGIHVLIEKPVTTTVS
                     EAESLLRMAADSDLVLQVGHIERFNSAVQYISKIVHEPLFLQSRRLGPFSPRISDVGV
                     VLDLMIHDIDIILSLVRSEITAISAAGRCIRSDHEDIASAQISFENGSMAHILVSRVS
                     ERRLRQLEIMEPERYVTIDYETQDVSINRCVRQNNSSLEVIEHPVFPKSEPLKLELQH
                     FVTCVREGKQPLVGITDGKRALEVAVSVLKQIHLNDESAACRGEAGVS"
     gene            4662..4880
                     /locus_tag="P5219_00960"
     CDS             4662..4880
                     /locus_tag="P5219_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliR"
                     /protein_id="HRX25356.1"
                     /translation="MPIVNVQALIALGMFLASLFIARIVVRIRNGSLPGGEMWVLYLR
                     MLLGFLLAGAVTLAFYSFAGVDVISKHL"
BASE COUNT         1065 a         1325 c         1429 g         1139 t
ORIGIN      
        1 cctctggcct ttttcgaccc tggggtggcc cgacgggacg gaagaccttg atttctatta
       61 ccccacgtcc ctcatggtca cgggcttcga catcatcttc ttctgggtcg cccggatgat
      121 catgatgggc ctcgagttca tggacgaggt tcccttcaga gatgtgtaca tccacgccct
      181 catccgcgac gagaaggggc agaagatgag caagtcgagc ggcaacgtca tcgaccccct
      241 cgacatgatc gacaaatacg gagcggatgc cctccggatg accctggcgg ccctcacggt
      301 ccagggacgg gacattcttc tgagcaccgg caagatagaa acctaccggc tgttcatgaa
      361 caaaatctgg aacgcgtccc ggtttgccct gatgaacctg gaagacggcg tccgcctttc
      421 ggagcttccg gccggctccc cgctggccct ccatgaccgg tggattcttg ccaggacgaa
      481 gatcgtctgc ggcgaagtgt cacggcttct ggacagcttc gggacgggtg aggcggcgcg
      541 ccttctgtac gatttcgtgt ggggggacct gtgcgactgg tatctcgagc tttccaagcc
      601 ggccctcagg ggcgatgaag gccccgaaag aaaacaggca acccagacag tgatttccgg
      661 agttttccgg gatgtcctgc tgcttcttca cccattcatt cctttcgtga ccgaagaact
      721 ctgggagacc ttcggtttcg gcgggggatt catcgaacag aatgcctggc cccggtggga
      781 agacaggccc gagtatccgg gagctgccga gaagatggag tttttccagg acacggtccg
      841 tatcctgaga aatctcaggg cggaatccag gatcgcaccc caggcatttg cgaaccaggc
      901 ctttgttcag acggaagaga attcccttct ggccgaagtg ctcagggaga acggaacaat
      961 catggaaacc ctcacaaaaa tacggaagat cacccttctt tcgccgaagg acgaaaaacc
     1021 ggccggttct ctctcgtccc agatggagag cggtgaagtc agtctcgtgg tgggggatat
     1081 cctggacatc ggtgcggaaa ttgaacgact gacccaggag atggctgcaa tccggaaaaa
     1141 tatcgaggcc ggaagaagaa agctggccaa cgaagatttc gtcagcagag cgccggcgga
     1201 agtagtggag aaagaaaagg acaggctcgc ggaaaatgaa gcccgccttt ccaggattga
     1261 agaaaacctg tcaagcctga gacgttcctg atactgccat gaacaggaag ccccggtcct
     1321 tcgaagaact ggagaacgtt ttttcttccc tcgccagtgc gggaatccgc ccggggctcg
     1381 accggatttc ccgcctgatg gagaagctgg gaaatcccga gaaggacttt ccggcagttc
     1441 atatcgtggg aaccaacgga aaaggttcca ctgcagccat caccgactcc atcctcaggg
     1501 agtcggggta caggaccgcc ctctatacca gcccccatct cgaatcccct gatgaacgcc
     1561 ttcttatcga cgggtctccc ctttctctcg gagaatggac catggcagcg gatgatattg
     1621 aaaaggcgtt ggaggcggat gttatcctgc gggccgatcc tcccaccttt tttgaacttg
     1681 ttacggcagc ggcgtttctt ctctgctcgc ggaacagggt ggaaattgcc gtggtggaag
     1741 ccggactcgg gggcaggctt gacgcgacga accttctgag cagcgtcgtc ctgacctttg
     1801 tgacctccat ttccatggac cacatggact tcctcggcga cacgattgaa aagattgcgg
     1861 gagaaaagtt cgcggttatg agggaaggag tacccgcgtt tttctcgggc agaccctcgg
     1921 ggcttgttcc tcttttccgg aaaaaagccc gtgactcagg cgcctttccc catgttcttc
     1981 ctgaagaaat ccgggtggag aacgtttccg tctcggagga ggggacagcc ttttcactcc
     2041 tctgcggggg ggaacgctat cagatacgta caccgctgca tggctccttt caggttgaaa
     2101 actgttccct ggcggttgcg ggtatgctcg gcatctccgg acgcttccac cgcatctcgg
     2161 aagaagccct tctacgggga acggagaagg cacgctggcc cggaaggttt gaaatactca
     2221 gggccgaccc tccgctggtc cttgacggag gccataaccc tgacgggatt atgaggcttg
     2281 tcgagagcct ccgggcgatt tacggcgaaa aaaagccgac ggtcgttttc gcctgtatga
     2341 aggacaagga ttttcccgca ggtctttccc tgctgctgaa tggagcgggc aggctgatct
     2401 gcacgtccgt tccgggcaac ccgagatctg ccgatcctta cgtgctggcg caggctgcga
     2461 agaacgccgg ctggaaagag gatgccgtgg aggtcgttcc ggatcccctg gatgccctga
     2521 aggcttctga acggacgaga cagggcacgg tatgctgcgg gagtctctat ttcatcggct
     2581 ggatgcgcac cctgatcttt tcacgaagag aggagctgca tttttgaaag tgaacggccc
     2641 ggcatacaaa attgaatgct cttcagtgaa cgattccatc gtttttctgc ctcccggaga
     2701 tgaaaaacgg ttttccataa gactcttcct gaagggagcc gccatgaacc gggcttccgg
     2761 cgttcccggc cgttccaggg agcttctgct tggcgccttc ccccggaaag gaccccttgt
     2821 ggccccgcag caggttcacg gaacggatgt tatcgcggct tccaccgggg caacccttcc
     2881 ggcccggccc agcgccgacg gagtttgtgt cgacaggagc ggcattgagg cttctcttcg
     2941 gtttgcagac tgttttccgg tggttatcgc ttccctttcc ccttctcccc ggatcattct
     3001 ccttcattcc ggatacaagg gtaccgtgca gaatattgcg ggcagggccg cccgttccct
     3061 tttgggggag aagggcgctg aaccttcgga aacctgggcc tggattggtc cgggaatcgg
     3121 aaaggagcat tatttcagaa aaaaaggcga accctggacg aagcgtggca ttgagtcctt
     3181 ttcccggcac tgtgtgtcgg aagacgggaa tgacgtcttt ttcgacctgg gcggcgaaat
     3241 ccggagacag cttaaaggtg ccgggattgt tgatgagatg atttgttccg tccccttatg
     3301 cacgttcagg gataatgatg tatgctattc atacagaaaa ggggacaggg aaaacaggct
     3361 ttttcttctt gcctggatgg aataggtttc tcctgccact tttgatgctt ctcccgtgag
     3421 aatactgtac ggggaaagtt tttttcaagc ctcaaaagga gttgatccgt tcatggacct
     3481 gaaaaggctg ggcgtcatcg gggtcgggca tctcggacag caccatgcta gggtatacac
     3541 agaactcctc gggacacagc tcgtaggtgt cgtcgatcag aacgaaagca gggcagccgc
     3601 cataggggac aatctgggtg ttccatggtt tactgacatg gatgagttta tcgcccgggc
     3661 aaggccggat gccgtgagcg tggtggttcc cacgagcctc catttcgaga tagcgaaaaa
     3721 agcgctgagc aacggtatcc atgtcctcat cgaaaagccg gtgacaacca cggtgagcga
     3781 agccgagagc ctgcttcgaa tggcggctga ctcagatctt gtccttcagg taggccatat
     3841 cgagagattc aacagtgccg tccagtacat ttcgaagatc gttcatgaac cccttttcct
     3901 ccagtcccgg agactcggcc ccttttctcc ccgcatcagc gacgttggag ttgtcctgga
     3961 cctgatgatt cacgacatcg acatcattct ttcccttgtc cggtcggaaa tcacggcgat
     4021 ttccgccgcc gggaggtgca tccggtccga tcacgaagac atcgcttccg cccagatctc
     4081 ctttgaaaac ggatccatgg cgcacatcct tgtaagccgg gtttcggaaa gacggctgcg
     4141 tcagctcgag atcatggaac ctgaacggta tgtgaccatt gactatgaga cccaggacgt
     4201 gtccatcaac cggtgcgtca ggcagaacaa cagcagcctg gaagtcatcg aacatcccgt
     4261 cttcccgaag agcgaacctt tgaaactgga acttcagcat ttcgtgacct gcgtccggga
     4321 agggaagcag ccccttgtgg gaatcaccga tgggaaacgg gccctggagg ttgccgtatc
     4381 ggttctcaag cagatccatc tgaacgatga atccgccgcc tgcaggggag aggcaggagt
     4441 gtcctgattt ccattccccc gttccggtga ccgaaatatc cagaaaaaat ggtatagtag
     4501 ataccctggg tgcagaatcc agggtatctt tttcgtggaa gaagaatttt tttctttttt
     4561 ttcttcgcct atttgctgaa aaaatctttt tttaagtggc gttccgtttt tttatggggt
     4621 atcctaattt ccattatcag aagctgaaag ttgagtgaag aatgcctata gtcaatgttc
     4681 aagcgctcat agccctcggc atgttccttg cgtccctgtt catcgcccgt atcgtggttc
     4741 gtatccgcaa cggatctctg ccgggagggg agatgtgggt actctacctg agaatgctgc
     4801 tgggttttct attggcgggg gcagtcacgc tcgcctttta ttcctttgcg ggcgttgatg
     4861 tcatttcgaa gcatttatga gtgagtttgc cggtttcagg aggaggagtg cggccgatgg
     4921 attgcgatag aagatggccc cgtcgtatgc tgtttctt
//
LOCUS       DAOTHD010000028         4915 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_20428_length_4915_cov_3.425309, whole genome shotgun sequence.
ACCESSION   DAOTHD010000028 DAOTHD010000000
VERSION     DAOTHD010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20428_length_4915_cov_3.425309"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            319..1299
                     /locus_tag="P5219_00965"
     CDS             319..1299
                     /locus_tag="P5219_00965"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRX25357.1"
                     /translation="MKKLLCLVAVCLVLFAAGTALAAEKIVVRVAHTIAPDSHYNKGL
                     EHLGKLLDEATKGQIELQIFHSSQLGSERDAIEGVSMGTLEMTLVSSAPLANFTNAFL
                     VFDLPFIIQDRQKAYAWMDGPEGRKILDSVLSKGMVGLGIWENGFRMLTNSKKPVAVP
                     DDLKGLKIRLMENPIHVGTFKTLGAYPVPMPFGELFTALQQSTVDGQENPLIIISTSK
                     FAEVQKHLSLTGHFYAPAILLINKDFWEKTLTEEQRKIFTEAELKARHWEREFCIEIE
                     KQLVGDLKAQGMVVTEPDKAKFFEAVQPVYKEFEEKVGKEAIQKLVDAQK"
     gene            1402..1896
                     /locus_tag="P5219_00970"
     CDS             1402..1896
                     /locus_tag="P5219_00970"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRX25358.1"
                     /translation="MRKIVDNFEEYALLLLFPLMVAVVFTATLARYLNLFPMFWGEEV
                     ARYIMVFMAYIGAGLGMKKGAHVGVSFFTDRFRNVKIRIFLEGFRLGIIVFFCGMIMY
                     YYRSIIVHQISMGQTTPALYIPMWVPYSAVPLGMFLVALRAFQAFRETTASIRSSAHM
                     TEVR"
     gene            1898..3184
                     /locus_tag="P5219_00975"
     CDS             1898..3184
                     /locus_tag="P5219_00975"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRX25359.1"
                     /translation="MAAIMFSVLILLIFLNIPVAVSIGLASIAGITFGNLPLNPVVVA
                     QRMFTSADSFPFMAIPFFMLAGGLMEHGGISRRLVKLASALVGGYRGGLGLITILASA
                     FFGAISGSNPATVAAIGGIMVPSMIKKGYPPAFAASIAAAGGTLGVVIPPSIPMITFG
                     VVAGVSIGDLFLAGFGPGVLLAVVLSLVVVVQSARLDIPREEPRTRGQLFAAVKDSIL
                     AIIMPLIILGGIYGGVFTPTEAGAVAVIYSLLISAFVYRELDLKTIRTVIIKAGISTS
                     VVFFVIATSQSMSWLITVSRVSAEIAAWIVSVSSNPFVLITLINLILLFLGVFLETQA
                     IILLMAPLLLPITASIGMNPLMLGIIMVVNTSVGMITPPMAVNLFVAVSLVKDHNVGL
                     EDITRKIFAFLVAEIIAVMFISNFPIFSLGLVELVK"
     gene            3189..4397
                     /locus_tag="P5219_00980"
     CDS             3189..4397
                     /locus_tag="P5219_00980"
                     /inference="COORDINATES: protein motif:HMM:NF018682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HRX25360.1"
                     /translation="MRAFAALCDDLTGSSVQSILLKDRGLSVRQIIRPGGELPVPEDG
                     EAIVINCDTRRRSPGEAQEIFRRFASLFPPSVGIGKRIDTTLRGHILDETAELLSARP
                     GAAALVVPAYPASGRTTVGGYHLLGGSLLERTEVARDPLWPVSSSYVPGYFTGTLPCG
                     LITMETVKLGPPAVAEALSGLLAGGTRVVAADAMTAEDIETLAEGSASVTAEIIPVDP
                     GPFTAAFLARKLPGRNTGTALAVIGSTSEKTARQIAWTEGKLRCSLFTLLPGERTDDA
                     LPRLRAFLADLHDHGDFLLIRPSPEIVRGSEAATAAVLADLGRETLRILGRKICGILL
                     SGGDTAAMFFEGSGTASLAPGEEIQPLMMGGRILDGEFAGLAAVTKGGLIGEEDGIYR
                     AVQWLKKERT"
     gene            4403..>4915
                     /locus_tag="P5219_00985"
     CDS             4403..>4915
                     /locus_tag="P5219_00985"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRX25361.1"
                     /translation="MTRTVIAVPMGDPAGVGPEIALRAMADPDVWKEGVPLLIGDLTV
                     LEAVSEKLSLPAKLRSVKDTSDIAGSPDELPVMDLGMVPDPASLQVGHVSALAGKASV
                     AYIRKAVELCMSGTAAGVATTPINKEALKAAKEPYIGHTEMLAEMSGATKSYTMFLVD
                     KLRILFHSRHL"
BASE COUNT          890 a         1663 c         1400 g          962 t
ORIGIN      
        1 acgggcagcg gagagcgtca ccggggcccg ccggatgaat acggggggaa gcgtcgtcca
       61 ggaaccacca ccggcttcac aggatgttcg ccttaattgt gaatatatta ataatatagc
      121 atcgaatctt ttttcggagt attattcttt tcacattgtc cggtgttgtg aagggcagtt
      181 tacgaacggg acgcccgctg atgccggatg ctgcggaaag gggatgacag ccgtatcaga
      241 aaagagaagc ccgtcctgtt ccttcagctt tgttccgtat cccaccgaaa agatccgtac
      301 acaaggagga ataaacccat gaagaagctt ctgtgtcttg ttgccgtctg cctggtcctg
      361 ttcgccgccg gaaccgccct tgccgccgag aagatcgtcg tccgggtggc ccacaccatc
      421 gcccccgact cccattacaa caagggtctc gaacacctcg gaaagctcct cgacgaggcg
      481 acgaagggac agatcgaact ccagattttc cattcgtccc agctcggatc ggagcgggac
      541 gccatcgaag gcgtctccat gggcaccctg gagatgaccc tcgtctcctc cgctcccctt
      601 gcgaacttca ccaacgcctt cctcgtcttc gaccttccct tcatcatcca ggaccgccag
      661 aaggcctacg cctggatgga cggccccgag ggaagaaaga tcctcgactc cgtcctctcc
      721 aagggcatgg tcggcctcgg gatctgggag aacggcttcc ggatgctcac caactccaag
      781 aagcccgtgg ccgttcccga tgatctcaag ggcctcaaga tccgcctcat ggagaacccc
      841 atccacgtgg gcaccttcaa gaccctcggc gcgtatcccg ttcccatgcc cttcggcgag
      901 ctcttcaccg ccctgcagca gagcaccgtg gacggccagg aaaatccgct gatcatcatc
      961 tccacctcca agttcgccga ggtgcagaaa cacctctccc tcacgggaca tttctacgcc
     1021 cccgccattc tgctgatcaa caaggacttc tgggagaaaa ccctcacgga ggaacagcgg
     1081 aagatcttca ccgaagccga gctgaaggca cgccactggg agcgggagtt ctgcatcgaa
     1141 atcgagaagc agctcgtggg cgacctcaag gcccagggca tggtagtgac cgagcccgac
     1201 aaggcgaagt tcttcgaggc agttcagccc gtctacaagg aattcgagga gaaagtgggc
     1261 aaggaagcca tacagaagct cgtggacgcc cagaaataac cgttccggcg gcataccgcg
     1321 gggcgttcct cgggaacgcc ccgctctttt cgccctctcc cgggcacatc aaaggagagg
     1381 ttttctgaca gggggaccgc tatgcgaaaa attgtcgaca attttgaaga gtacgccctt
     1441 cttctgctct ttcccctcat ggtggccgtg gtcttcaccg ccaccctcgc ccggtacctg
     1501 aacctctttc caatgttctg gggcgaggag gtggcccggt acatcatggt cttcatggcc
     1561 tacatcggtg cggggctcgg catgaagaaa ggggcccacg tgggggtcag cttcttcacc
     1621 gaccggttcc ggaacgtgaa aatccggatc ttcctggaag gattccgtct cggcatcatc
     1681 gttttcttct gcggcatgat catgtactac taccggagca tcatcgtcca ccagatttcc
     1741 atgggccaga ccactcccgc cctctacatc cccatgtggg ttccctattc ggctgttccc
     1801 ctcgggatgt ttctcgtggc cctccgggct tttcaggcct tccgtgagac gacggcttcc
     1861 atccggtcct ccgctcacat gacggaggtg agatagcatg gcagcgatca tgttttccgt
     1921 cctcatactc ctcatcttcc tgaacatccc cgttgcggtg agcatcggcc tcgcctccat
     1981 cgcggggata accttcggga acctccccct caaccccgtc gtggtggccc agcgcatgtt
     2041 cacatccgcc gactcctttc ccttcatggc cattcccttc ttcatgctgg ccggcggcct
     2101 catggagcac gggggcattt cccgccgcct ggtcaaactg gcctccgccc tggtcggggg
     2161 ctacagggga gggctcggac tcatcaccat cctcgcgagc gcattcttcg gggccatcag
     2221 cggatcgaac cctgccacgg tggccgccat cggcggcatc atggttccct ccatgatcaa
     2281 gaagggctac ccgcccgcct tcgccgcctc catcgcggcg gcgggaggca ccctcggcgt
     2341 ggtcatcccg ccgtccattc ccatgatcac cttcggcgtg gtggccgggg tctccatcgg
     2401 cgacctcttc ctcgcgggct tcggccccgg cgtcctgctg gccgtggtgc tcagcctggt
     2461 ggtggtcgtc cagtccgccc ggctcgacat ccccagggag gaaccccgca cccgcgggca
     2521 gcttttcgcc gccgtgaaag attccatcct ggccatcatc atgcccctca tcatcctcgg
     2581 cggcatctac ggaggagttt tcacccccac cgaggccggc gccgtggctg tcatctacag
     2641 cctcctgatc agcgccttcg tctaccggga actggacctg aagaccatcc ggacggtgat
     2701 catcaaggcg gggatcagca catcggtggt ctttttcgtc atcgccacgt cccagtccat
     2761 gtcgtggctc atcaccgtca gcagggtgtc ggcggagatc gccgcctgga tcgtctccgt
     2821 ctcctccaac ccctttgtcc tgatcaccct gatcaacctg atcctccttt tcctcggcgt
     2881 gtttctcgag acccaggcca tcattctcct catggcgccc ctgctccttc ccatcacggc
     2941 gtccatcggc atgaacccgc tgatgctggg catcatcatg gtggtgaaca cctccgtggg
     3001 catgatcacg cctcccatgg cggtgaacct cttcgttgcc gtgtccctgg tgaaggatca
     3061 caatgtgggc ctcgaggaca tcacccggaa gattttcgcc ttcctcgtgg cggagatcat
     3121 cgccgtcatg ttcatcagca atttcccgat cttctccctg ggactcgtcg agctggtgaa
     3181 gtaggatcat gagagccttt gccgccctct gcgacgatct caccggctcg tcggtccagt
     3241 ccatcctcct gaaggacagg gggctctccg tccggcagat catccgtccg ggcggagagc
     3301 tccccgtccc ggaggacggg gaggccattg tcatcaactg cgacacccga aggcgctccc
     3361 ccggcgaggc tcaggagatc ttccgccggt tcgcctccct ctttcctcct tcggtaggga
     3421 tagggaaacg catcgacacc actctgcggg ggcacatcct cgacgagacg gcggaactcc
     3481 tctccgcccg ccccggagcc gccgccctgg tggttcccgc ctaccccgcc tcgggaagga
     3541 ccaccgtggg agggtaccat ctcctggggg gctccctcct cgagcggacc gaagtggccc
     3601 gggaccctct ctggcccgtg tcgtcaagct acgtcccggg ctatttcacc ggaacgcttc
     3661 cctgcgggct catcaccatg gaaacggtga aactcggtcc cccggccgtg gccgaagcgc
     3721 tctccggact tcttgccggc ggaaccaggg ttgtggcggc cgacgccatg acggccgagg
     3781 acatcgaaac cctggcggaa ggctccgcct ccgtgacggc agagatcata cccgtggatc
     3841 ccggtccctt caccgccgca ttcctggccc ggaagctccc cggaaggaat accggaaccg
     3901 ccctggcggt catcgggagc acctcggaaa aaacggcccg gcagatcgcc tggacggagg
     3961 ggaaactgcg ctgctccctg ttcaccctcc tgcccggtga gcggaccgat gacgccctgc
     4021 cgaggcttcg cgcttttctc gcggacctcc acgaccatgg agattttctg ctgatccgcc
     4081 cctccccgga gatcgtcagg ggctccgagg ccgcaacggc ggcggtgctg gccgatctcg
     4141 ggcgggagac tctccggatc ctgggaagga aaatatgcgg tatacttctc tccggcggcg
     4201 ataccgccgc catgttcttt gaaggttccg gaacggcctc ccttgccccc ggagaggaaa
     4261 tccagcccct gatgatgggc gggcggattc tcgacgggga gttcgccggg ctcgcggcgg
     4321 tgaccaaggg gggcctcatc ggcgaggagg acggcatcta ccgggccgtc cagtggctga
     4381 aaaaggaaag gacttgattg caatgacacg tacagtgatc gcggtaccca tgggcgaccc
     4441 cgcaggggtg ggccctgaaa tcgcccttcg ggccatggcc gacccggacg tctggaaaga
     4501 gggcgtcccg ctcctcatcg gcgacctcac cgtcctggag gcggtgagcg aaaagctctc
     4561 ccttccggca aagctgcgca gcgtgaagga cacatctgac attgccgggt cccctgacga
     4621 acttcccgtg atggacctcg gcatggtccc cgacccggca tccctccagg tggggcatgt
     4681 gtccgccctg gccgggaagg cgtcggtggc ctacatcagg aaagccgtgg aactctgcat
     4741 gtccggcacg gccgcggggg tggccaccac ccccatcaac aaggaagccc tcaaggcggc
     4801 gaaggaaccc tacatcggcc acacggagat gctcgccgaa atgtccgggg cgaccaaaag
     4861 ctacaccatg ttcctggtgg acaagctccg cattctcttc cacagccggc acctc
//
LOCUS       DAOTHD010000029         4862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_20735_length_4862_cov_3.255253, whole genome shotgun sequence.
ACCESSION   DAOTHD010000029 DAOTHD010000000
VERSION     DAOTHD010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20735_length_4862_cov_3.255253"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..904
                     /locus_tag="P5219_00990"
     CDS             98..904
                     /locus_tag="P5219_00990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRX25362.1"
                     /translation="MGGYFENKVAAVTGAASGLGLGITESLLARGAGAAFMGDLKEEN
                     LTRESSRLSTEYPGKVFPVLTDVTKPEEVRKLVGDARDFDGHLDFLFNNAGVGMTLPT
                     EQITFEMWKFVVDLNLMGTVYGTYSAIPIMREQGFGHIVNTGSITGRVPVPYQAVYAA
                     TKSAVISMTESLQYELENEGLRFSVFCPANVRTAIFGDMTPPPDSISVEEAVEYVFDE
                     MEKKSVVIILPQSARDFDTLYRTKKDMFDDFARKLADERRENYRTKGTYY"
     gene            complement(994..1698)
                     /locus_tag="P5219_00995"
     CDS             complement(994..1698)
                     /locus_tag="P5219_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HRX25363.1"
                     /translation="MDFFFAHVPASGSILNALAIVIGSLAGLALHSRLPQRFVSITFQ
                     GLGLLTLFLGVFMAQRTQSFLVMAFSIVGGSLLGEYLDLEKGLNRLGEWTKAKIRSEN
                     EKFTEAFVASSLLFCVGSMAILGAIEEGMGEFPRLFVTKSLMDGMASAAMAASMGPGI
                     VFSALPVLLYQGGMTFLAGTVQTVMTQPVIDEVSAVGGLLLLGIGLSILEIKRVKAVN
                     MLPALLLAGILASFFR"
     gene            complement(1744..2673)
                     /locus_tag="P5219_01000"
     CDS             complement(1744..2673)
                     /locus_tag="P5219_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRX25364.1"
                     /translation="MEMIIPILTAAVRSGTPILYATLGEILTEKGGILNLGLEGIMLM
                     GAYTGFSVAYSTGSPELGVAAAFLVGALMGLVHAFICVSLKGNQVVSGLALTMFGTGA
                     SSLLGRTFVGFTIPGLPRISVPLLSDIPVLGPVFFSHDALTYCSYGLVVFFCWFFSST
                     RQGLYLRAVGDNPRAADAMGISVTKTRYFYTLAGAGIVAVGGAYMSVAYTKMWGELMT
                     AGRGWIAVALVIFAIWNPARAALGAYLFGGVEAFQLRLQTAGTTIPTPVLMMLPYLMT
                     IAVLLFISIKKGKGIIFGAPASLGLPFFREERD"
     gene            complement(2675..3760)
                     /locus_tag="P5219_01005"
     CDS             complement(2675..3760)
                     /locus_tag="P5219_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRX25365.1"
                     /translation="MRIKIEPRLTSTWKLSVAIPFFSLAAALFSGGVFLFFMGVSPLQ
                     AYRAIITSALGDTYGLSETVVKAIPLIMAGIAVMLSFTMLIWNIGAEGQIFMGAIAAT
                     AMVRFFYSDSRIVMLLLMFLAAAAAGGTWAAIAGYFRAKWNVNEIITTLMLNYVAMNF
                     LNYFVFGPWRDPSSLGFPMTPPFPDSARLPVFWGTRIHGGIFLALLLPLLFWIVLKFT
                     RWGYEIRVMGENPKAAGFAGMNYLKNVVLVMFISGAIAGIAGMCELAGLQGRLQHGFS
                     GGYGFTAIIVAWLARLHPIAIIFVSFLMGVLLVGGETLQIVMRLPLSSVMVLQGLILF
                     FVLGGEFFRKYTLRFIPARETPRKEGA"
     gene            complement(3757..>4862)
                     /locus_tag="P5219_01010"
     CDS             complement(3757..>4862)
                     /locus_tag="P5219_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRX25366.1"
                     /translation="VAILQMLFRQANILILDEPTAVLTPQEAEHLFETVRQMTSEGHG
                     VVFISHKMDEVMGLSQRVTILRKGKLVGTVETASTSKEQLAEMMVGQKMVYTISKTPL
                     EPGEPVLECASVCALGDRGFPAVNRATFMVRGREILGVAGVAGNGQQELCETLVGLRK
                     VESGSISVNGREMTNALPKDFIGQGVHYIPADRKGTGLVPNMNVSENSILKRYWNRPV
                     SRGLFLNWKEVFAFARELVSRFSVSTPSMETPVRNLSGGNLQKLMLGRELSDSPAALL
                     AVHPTWGLDVAATKFVREQLLSHRERGGAVLLVSEDLEELLALSDRLAVMCKGEIMGI
                     LDNPSTVPVETIGLMMAGTPLAELERAEGGKSL"
BASE COUNT         1081 a         1386 c         1486 g          909 t
ORIGIN      
        1 cgtgaggaag atggccggtg gggccatgat caagggagcc gcacggctga acgtgggcga
       61 ctcgagaaga ctgccgtagg aaaggggagg agaagacatg ggcggatatt tcgaaaacaa
      121 ggtggccgca gtcacgggcg ctgcctcggg attggggctc ggtatcactg aaagcctgct
      181 ggccagagga gccggcgccg ctttcatggg cgatctcaag gaagaaaacc ttacaaggga
      241 atccagcagg ctgagcaccg aatatccggg caaagttttt cctgtgctta cagacgtaac
      301 aaagccggaa gaagtcagga agctcgtcgg cgatgcgagg gatttcgacg gacacctcga
      361 ttttcttttc aacaacgcag gcgtgggaat gaccctcccc acggagcaga ttaccttcga
      421 gatgtggaaa ttcgtggtgg acctcaacct catgggtacc gtctacggaa cctacagcgc
      481 aatccccata atgcgggagc agggctttgg tcatatcgtc aacaccgggt caatcaccgg
      541 gcgggttccc gttccctacc aggcggtcta tgccgccacc aagagcgccg tcatatccat
      601 gacggaaagc ctccagtacg agctggagaa cgagggactc cggttcagcg tcttctgccc
      661 cgccaacgtc cggacggcga tcttcggcga tatgaccccc ccgcctgatt ccatcagcgt
      721 ggaggaggcg gtggagtacg tttttgacga gatggagaaa aaatctgtgg tgataatcct
      781 tccccagtca gcccgggatt tcgataccct ttacaggacg aagaaggaca tgttcgatga
      841 ctttgcccgc aagctggcgg atgagcggag ggaaaactac aggacaaagg gaacctatta
      901 ttgagcatcg tcgttggaat gtgatcttcg gcgggtgcct aaaaaaacga ggggagtccc
      961 ttcagggggc tcccctcgtt ccgtagaccg ggcctatcgg aaaaaggacg cgagaattcc
     1021 ggcgagcaga agggcgggaa gcatgttcac cgccttgacc cttttgatct ccagtatgga
     1081 aaggccgatg ccgaggagaa ggagaccgcc cacggcgctc acctcgtcga tgacgggctg
     1141 ggtcattacg gtctgcaccg tccccgccag gaaggtcatc cctccctggt agagcaggac
     1201 cggaagggcc gagaatacga tcccgggtcc catggaggcg gccatggctg ccgacgccat
     1261 tccgtccatg agcgacttgg tgacgaagag ccttggaaac tcccccatgc cttcctcaat
     1321 ggccccgaga atggccatgg aaccgacaca gaagagaagg gaggaggcca cgaaggcctc
     1381 ggtgaacttt tcgttctccg agcggatttt tgctttcgtc cattccccaa ggcggttcag
     1441 tcccttttcg agatccaggt attctccgag gagggatcct cccacgatgc tgaatgccat
     1501 gacgagaaag ctctgggtgc gctgggccat aaaaacgccg agaaacaggg tcagcaatcc
     1561 cagtccctgg aaagttatgg agacgaacct ctggggaagg cgcgaatgaa gagccagccc
     1621 cgcaagggag ccgatcacga tggcgagggc gttcagaatg cttcccgagg caggaacatg
     1681 ggcaaaaaag aaatccacga tattgaatcc tcctgtactt ttcagctgac gggaaaaagg
     1741 gaatcagtcc ctttcctccc gaaaaaaggg taatccgagg gatgccgggg ctccgaaaat
     1801 gatgcctttt cccttcttga tcgagatgaa gagcagcacg gcgatggtca tcagataggg
     1861 aagcatcatc agcaccgggg tcgggatggt ggttcccgcc gtctggagcc gaagctggaa
     1921 agcttcaacg ccgccgaaga gataggcgcc gagggctgcc cgggcagggt tccagatggc
     1981 gaagatgacc agcgccacgg cgatccatcc ccgtcccgcg gtcatcagct ccccccacat
     2041 ctttgtgtag gccacggaca tgtaggcccc cccgactgcc acgatgcccg cccctgccag
     2101 ggtgtagaaa tagcgggtct tcgtcacgga aatgcccatg gcgtccgctg ccctggggtt
     2161 gtctcccact gcacggagat agagcccttg ccgtgtcgac gagaagaacc agcagaaaaa
     2221 gacaaccagc ccgtaggagc agtaggtcag ggcgtcgtgg gaaaagaaca cgggtccgag
     2281 caccggtatg tctgagagga ggggtacgct gatccgtgga aggcctggaa tggtgaatcc
     2341 cacgaatgtc cttcccagca gggagcttgc gcccgttccg aacatggtga gggcgaggcc
     2401 gctgacgacc tggtttccct tcagcgaaac gcagatgaag gcgtggacga ggcccatgag
     2461 ggcacccacc agaaatgccg ccgccacccc gagctccggg ctgcccgtgg aataagctac
     2521 ggaaaacccg gtgtacgctc ccatgagcat gatgccttcg aggccaaggt tgaggatccc
     2581 gcccttttca gtgagaattt cccccagggt ggcgtagaga atgggggttc cgctcctgac
     2641 tgcggcggtg aggatgggga tgatcatttc catgttatgc cccctccttc cggggagttt
     2701 ctctcgccgg gatgaacctc agggtatatt tccggaagaa ttccccgccg aggacgaaga
     2761 acaggatcaa tccctggagc accatgacgc ttgaaagcgg gagacgcatg acgatctgga
     2821 gcgtttcgcc ccccacgagc aggacgccca tcaggaagga gacgaaaatg atggcgatgg
     2881 gatggagcct ggcgagccag gcgacgatga tggcggtgaa gccgtacccc cccgagaagc
     2941 cgtgctgcag cctcccctgg agcccggcga gttcgcacat tcccgcgatc cctgcgatgg
     3001 cgccggaaat gaacatgacg aggaccacgt tcttcagata gttcatccct gcaaatccgg
     3061 ccgccttcgg gttctcgccc atgaccctga tttcgtaccc ccagcgggtg aatttcagga
     3121 cgatccagaa aagaagggga agaagaagag caaggaaaat gcccccgtga atgcgggtgc
     3181 cccagaagac ggggagcctg gctgaatcgg gaaagggagg cgtcatggga aagcccaggc
     3241 ttgaggggtc tctccagggg ccgaagacga agtagttgag aaagttcatg gcaacgtagt
     3301 tgagcatgag tgtcgtgatg atctcgttca cattccattt cgccctgaaa tatcccgcta
     3361 tggccgccca ggttcccccg gcggcggcgg cggcaaggaa catgaggaga agcatgacga
     3421 tcctgctgtc ggaatagaag aaacggacca tggccgtcgc cgctatggca cccatgaaga
     3481 tctggccttc ggcgccgatg ttccatataa gcatggtgaa ggagagcatg acggcgatgc
     3541 ccgccatgat cagcgggatg gcctttacca ccgtttcgct gagaccgtag gtgtctccca
     3601 gggcggaggt gatgatcgcc cggtaggcct ggagggggga gactcccatg aaaaaaagaa
     3661 atacccctcc tgaaaaaagg gcggcggcca gggagaaaaa gggaatggcg acggagagct
     3721 tccaggtcga ggtcagacgg ggctcaatct tgattctcac agggatttcc ccccttccgc
     3781 cctttccagt tccgccagcg gagtacctgc catcatcagt ccgatggtct ccaccggcac
     3841 tgttgaagga ttgtcgagaa tacccatgat ctcccccttg cacattaccg ccagcctgtc
     3901 gctcagggcg agaagttctt cgagatcctc cgacacgagg aggaccgctc ctccccgttc
     3961 cctgtgggag agcagctgct ccctgacgaa ttttgttgcg gccacgtcga gtccccaggt
     4021 ggggtgcacg gcaagcaggg cggcgggaga atcgctcagt tccctgccga gcatgagctt
     4081 ctgcaggttc ccccccgaga ggttacgcac cggggtttcc atggaggggg tgctcacgct
     4141 gaaccttgag acaagctccc gggcgaaggc aaacacttcc ttccagttca ggaacagtcc
     4201 cctggagacg gggcggttcc agtaccgctt caggatggaa ttctcgctta cattcatatt
     4261 gggaacgagt cccgtccctt tcctgtcggc agggatatag tgcactccct gcccgataaa
     4321 gtccttcggg agagcgttgg tcatctcccg gccgttgacg ctgattgacc ccgattccac
     4381 ttttctcagc cccacgaggg tttcgcacag ttcctgctgt ccgtttcccg ccactcctgc
     4441 tacgcccaga atctcccttc cgcgcaccat gaaggtcgcc cggtttacgg caggaaaccc
     4501 tctgtcgccg agggcgcaga cggaggcgca ctccagtacg ggctctcccg gttccagggg
     4561 agtcttggaa atggtgtata ccatcttttg tcccaccatc atctctgcga gctgctcttt
     4621 ggacgtggag gcagtctcca cggtgccgac gagctttccc ttcctgagga tggtcaccct
     4681 ctgcgaaagg cccatgacct cgtccatttt gtgggagatg aagaccacgc catgcccctc
     4741 ggaggtcatc tgccgcacgg tttcaaagag gtgctccgcc tcctgggggg tgagcaccgc
     4801 cgtgggctcg tccagtatca ggatgttcgc ctggcggaag agcatctgga gaatggccac
     4861 cc
//
LOCUS       DAOTHD010000030         4774 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_21205_length_4774_cov_2.813944, whole genome shotgun sequence.
ACCESSION   DAOTHD010000030 DAOTHD010000000
VERSION     DAOTHD010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21205_length_4774_cov_2.813944"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(291..764)
                     /locus_tag="P5219_01015"
     CDS             complement(291..764)
                     /locus_tag="P5219_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRX25367.1"
                     /translation="MAVSGCFPLPVVFCFLLGEKGVLLYRRMTAPWNGTVTVPGGKKE
                     LGETAREACIREIEEETGYRLRSLRLRGIAHILSGENEATGYYFSSRDFEGELRDSCE
                     GVPFWHPAEESLSLEGINPFYSLLAPKILDETVPLFEAKILSDDRGICSFSFVNA"
     gene            complement(769..1575)
                     /locus_tag="P5219_01020"
     CDS             complement(769..1575)
                     /locus_tag="P5219_01020"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRX25368.1"
                     /translation="MIRVTSSAPLTGVSVQWQGRNIPLDVALWNNHYIALGLFGTQTG
                     KVKPGLHTMKVTVNSNGTRRRISLPIRVTPVKYREDHLTLPESMVTPPANVLARIAKE
                     RKAVGKALSTATLTREWGLPLVRPVDGIVTSPYGRRRILNKKPRTPHGGMDFRAAQGT
                     PVRAALPGRVVLAGDHYFAGKSVYVDSGGGVISHYFHLDSIGVKEGERINRGAVIGKS
                     GMTGRSTGPHLHFGLSLSGQLVNPEPLFDGTISGMLEKTVSRTLNITEGK"
     gene            complement(1691..3016)
                     /locus_tag="P5219_01025"
     CDS             complement(1691..3016)
                     /locus_tag="P5219_01025"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HRX25369.1"
                     /translation="MDMLGFIERKRDGGVHQGEEIVRFVKAFASGEVPDYQAAAWLMA
                     VYFNGLSGEELRDFTLALARSGETLSFGDDLFLVDKHSTGGVGDKITLLLVPLAAACG
                     LSVAKLSGPGLGFTGGTVDKLEAIPGFRTHLPSEEFLRQVKNVGCAISGHSADLAPAE
                     GKFYSLRDVTATVPSLPLICSSIVSKKIAGGADAFVFDVKCGSGAFMEDYGSAERLAE
                     ALVSLSKSLGKKAMALITDMDQPLGEWVGNALEVLEAVRVLRGGGPADVREVAVSLAG
                     AMVSLGKGVSFEDGCALAAEKLDDGSALAKMKVLVEAQGGDGRVCEDPETVLSIAPEK
                     AFVKAKSGGRLARIDARAVGEGVKRLGGGRMTLGEPIDLSVGALMLVKTGADVSAGDA
                     LLEIRSSCQDKLKASLPFFEKAFFVEKTTLDSPRKGEEMKRSFVLGTIR"
     gene            complement(3018..3839)
                     /locus_tag="P5219_01030"
     CDS             complement(3018..3839)
                     /locus_tag="P5219_01030"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRX25370.1"
                     /translation="MEMQDRVQKALDYVRSRSGGVPEAAVVLGSGLGAFADALEDRIS
                     IPYGDIPGWPVSTAPGHAGRLVLGKIAQKYVAVMQGRVHYYEGYSMEDVVFPVRVFGA
                     MGVKTYVATNAVGAADHALSPGDVVLLYDHINWMGANPLRGPDTPGWNPRFPDMTHAY
                     DRKLIEAAERAAEKEGILVRRGVYMAFSGPSFETPAEVRMARLLGATVVGMSTVPEVI
                     TANAMGMRVCGISCVANHGAGMTENTLTGEEVLEEMAKASDKLVRLLKGFFGEME"
     gene            complement(3867..4760)
                     /locus_tag="P5219_01035"
     CDS             complement(3867..4760)
                     /locus_tag="P5219_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRX25371.1"
                     /translation="MRLYDFEASVENFLSYLLLERGLSHNTIAAYRRDLREWGGFCSR
                     EGALPLPPGQEILSLFQRYLARSEKSAATKQRIIAALRTWMRYLEQEGEGGGDVRLPS
                     LPGRKEGLPRILSEGEVDRLFQACEGDGPLDLRDRALFGTAYGCGLRAGELCGLRIGD
                     VDFSARVLRAFGKGEKERAIPFLGEVSEKVALYLQKGRPLLLKVPGDFLFLTRSGNPM
                     KREDIWRTLRKRGAKAGIPAARLHPHVLRHSFATHLLRRGMDQRSLQELLGHSSIATT
                     EKYLHFDLELRDVYDKAHPRA"
BASE COUNT          963 a         1542 c         1361 g          908 t
ORIGIN      
        1 aacagcaggc ggtcgtagac gactcccccg ttctcagccc gttcaaggca gaccgtcagg
       61 gtgggaacgg tgccccggcg cagttcttct tccgtggctg aaagcaccgg aaggaccgtc
      121 tccgccttga ttctttccga gaaataccgg gcgaacaggt cctcttccac ggaggaggcc
      181 gccgcagggc aggccaggaa gaccgcaaaa aaaacaggaa cgaaaaacaa aaaacttctt
      241 cggcacatca tcgtcacctc accggaaaag cgagccctcc atgatcagtc tcaggcattt
      301 acgaaggaga atgagcatat cccccggtca tccgagagaa tcttcgcctc gaagagagga
      361 accgtttcgt ccagtatttt tggcgcgaga aggctgtaga aggggttgat tccctcaagg
      421 gacaggctct cctcggcagg atgccagaaa ggcacaccct cgcagctatc cctcagttct
      481 ccttcaaaat ccctggagga aaaataatag cccgtggcct cgttttcccc ggagaggatg
      541 tgggcgatac cccggaggcg aagtgaccgg agacggtatc ccgtctcctc ctcgatctcc
      601 cggatgcagg cctcccgggc tgtttccccc agttccttct ttccaccggg gacggtcacg
      661 gtgccgttcc acggagcggt catcctccgg tagagcagaa cccctttttc tccaagcaaa
      721 aagcagaaaa cgacgggaag ggggaagcat ccggaaacgg ccatggcctt atttgccctc
      781 cgtaatgttc aacgttctgc tgaccgtctt ttcgagcatg ccggatatgg tgccgtcgaa
      841 aagaggttcg gggttcacga gctgccccga gagactgagc ccaaagtgca gatggggtcc
      901 cgtggagcgc cccgtcatgc cgctttttcc tataaccgcg cccctgttta tccgctctcc
      961 ttccttcacg ccgatggaat cgagatggaa gtaatgggag atcaccccgc cgccggaatc
     1021 gacgtagacg ctctttccag cgaagtaatg gtcccccgca agaacgactc tgccgggaag
     1081 ggctgccctg accggagttc cctgggccgc acggaagtcc attcccccat ggggggttct
     1141 cggcttcttg ttcagaatcc tccgccgccc gtagggactt gtcacgatac cgtccacagg
     1201 cctgacaaga ggaagccccc attcccgggt cagggtggcc gtggaaagag ctttgcccac
     1261 ggctttccgc tccttggcga tccgggccag gacattcgcc gggggcgtca ccatgctctc
     1321 cggaagagtc aggtgatcct ccctgtactt caccggggtc acccggatgg gaagggatat
     1381 ccggcgacgg gttccattgg aattgacggt caccttcatg gtgtggaggc cgggcttcac
     1441 cttccccgtc tgggtaccga acaggccgag ggcgatatag tgattgttcc acagggccac
     1501 atcgagagga atgttccgcc cctgccattg caccgacact cccgtgaggg gggcggacga
     1561 ggtcacccgg atcacgaaag ggctccccga tccgacggat gaagggtact gaacgttcgc
     1621 ccgggccagg gcttcgccgg gaaaaagaaa cacgagcagg aaaagaaaac acaccgaagc
     1681 ccaacgcatt ctacctgata gtcccaagaa caaaactcct tttcatttcc tctcccttcc
     1741 ggggagagtc cagtgtcgtc ttttcgacga aaaacgcctt ctcgaaaaat ggaagggacg
     1801 ctttcagctt gtcttggcag ctggagcgta tttccagcag ggcgtccccg gcggagacat
     1861 ccgctcctgt tttcacgagc atgagggccc ccacggagag gtcgatgggc tccccgaggg
     1921 tcatgcgacc tcctccgaga cgcttcaccc cttcaccgac agcccgggca tcgatcctgg
     1981 ccagcctgcc gccggacttg gcctttacaa aggccttttc cggagcaatg gacaggacag
     2041 tttcgggatc ttcgcatacc cgtccgtcgc cgccctgggc ttccacgaga accttcattt
     2101 ttgcaagggc gctcccatcg tccagttttt cagcagccag agcgcatccg tcttcgaagg
     2161 aaacgccctt cccaagggat accatggctc ccgccaggga aacggccact tcccgcacat
     2221 ctgcagggcc gccgcccctc aggacccgga ccgcctcgag cacctccaag gcgttcccga
     2281 cccattcgcc caggggctga tccatgtcgg tgatcagcgc catggctttt ttccccaggc
     2341 ttttcgagag ggacacgagg gcctcagcga gtctttcagc gctgccgtaa tcctccatga
     2401 aagcgccgct gccgcacttc acgtcgaaaa caaaggcatc cgcaccgccg gcgatctttt
     2461 tgctcacgat gctgctgcag atcaggggga gggacgggac cgtggcggtg acatcccgga
     2521 gagaatagaa ttttccctcg gcgggagcca ggtcggcgga atggcctgaa atggcgcagc
     2581 ccacattctt gacctgtcgg aggaactcct ccgacggaag gtgggtccgg aaccccggaa
     2641 tggcctccag cttgtccacc gtgccgcccg taaagccaag cccggggccg gaaagcttgg
     2701 ccaccgacaa cccgcaggcc gccgccaggg gaaccagcag gagggtgatc ttgtccccca
     2761 cccctccggt gctgtgcttg tccaccagga aaagatcgtc gccgaaggag agtgtttctc
     2821 ctgatcgggc gagggcaagg gtgaagtccc tgagttcctc gccgctgagc ccgttgaagt
     2881 agaccgccat gagccaggcc gccgcctggt agtcgggaac ctccccggat gcgaaagcct
     2941 tcacgaaccg gacgatctcc tcaccctgat gaacgccgcc gtctctcttc cgctcgatga
     3001 acccaagcat gtccatgcta ttccatctcc ccgaaaaatc ccttcagaag gcgtaccagc
     3061 ttgtccgagg cctttgccat ctcctcgagc acctcttcgc cggtcagggt gttttccgtc
     3121 atccccgccc cgtggttggc cacgcaggaa ataccgcaga ccctcatgcc catggcgttc
     3181 gccgtgatga cctcgggtac cgtggacatg cccaccaccg tcgccccgag cagcctggcc
     3241 attcggacct ccgcaggcgt ctcgaaggac gggccggaga acgccatata cacccctctc
     3301 ctgacgagaa tgccttcctt ttcagccgcc cgctccgcag cctcgatcag cttcctgtcg
     3361 taggcgtggg tcatgtcggg aaacctcggg ttccacccgg gggtgtccgg tcccctcagg
     3421 ggattggccc ccatccagtt gatgtggtcg tacagaagca ccacgtcacc gggagaaagg
     3481 gcatggtccg cggcacccac cgcattcgtg gccacgtaag ttttcacgcc catggcccca
     3541 aacacccgca cggggaaaac cacgtcctcc atggaatacc cctcgtaata atgaacccgc
     3601 ccctgcatga ccgcaacgta tttttgcgca atttttccaa ggacaagcct tccggcgtgc
     3661 cccggggccg tggacacggg ccagccggga atatccccgt aaggtataga aatcctgtct
     3721 tcaagggcat cggcgaaggc gccgaggccc gaaccgagaa caaccgccgc ttcgggcacc
     3781 ccgccggaac gggaacgaac atagtcgagg gctttctgga ccctgtcctg catttctatt
     3841 cctcctctcg aaaaaaggga aggggctcac gccctgggat gggctttatc ataaacgtcc
     3901 cgcagctcga ggtcaaagtg caggtacttc tccgtggtgg ctatggacga atggccgagc
     3961 agctcctgca ggctcctctg gtccatcccc ctccggagca ggtgggtggc gaagctatgg
     4021 cgcagcacat gggggtggag gcgggctgcc gggattccgg ctttcgcccc ccgtttgcgg
     4081 agagtcctcc agatgtcttc cctcttcatt ggattgccgc tgcgggtgag gaacagaaaa
     4141 tctccgggaa ctttcagcaa aaggggcctg cccttttgaa gatagagagc gaccttttcg
     4201 ctcacttctc cgaggaaagg gatcgcccgc tccttttctc ccttgccgaa agcccggagc
     4261 actctcgccg agaaatccac gtccccgatc ctgaggccgc acagctcccc cgcacgcagc
     4321 ccgcagccgt aggcggtccc gaaaagggcc cggtccctca ggtcaagggg accgtcgcct
     4381 tcgcaggcct ggaagaggcg gtccacctcc ccttcgctga ggatgcgggg cagtccttcc
     4441 ttccgcccgg ggagagaggg aagacggaca tcaccgcctc cctcgccctc ctgttcgaga
     4501 tagcgcatcc aggttctcag ggcggcgatg atcctctgct tcgtggcggc gcttttctcg
     4561 cttcttgcca ggtaccgctg gaacagggag aggatttcct gccccggggg aaggggcagt
     4621 gcgccctcac gggagcagaa gccgccccat tcccggagat cccggcggta ggcggcaatg
     4681 gtattgtgac tcaggccccg ttccaggaga agataggaca ggaaattttc caccgaagct
     4741 tcaaagtcat ataatctcat aatagttact catg
//
LOCUS       DAOTHD010000031         4747 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_21361_length_4747_cov_3.049020, whole genome shotgun sequence.
ACCESSION   DAOTHD010000031 DAOTHD010000000
VERSION     DAOTHD010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21361_length_4747_cov_3.049020"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="P5219_01040"
     CDS             complement(<1..226)
                     /locus_tag="P5219_01040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662142.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the B subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HRX25372.1"
                     /translation="MTALYREGFRRIEGISGPLLFVRGISRPACGELVAVESGAGRRA
                     GRILQIDGDLSVIQVFEGTMGLAPGSATVWL"
     gene            complement(223..>720)
                     /locus_tag="P5219_01045"
     CDS             complement(223..>720)
                     /locus_tag="P5219_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRX25373.1"
                     /translation="HFPAINWSQSYTLYDGMLAPWFRENLGEEWFALRDYLKDLLERE
                     QSLLELVQLVGRDGLSEEDKWTLFMAELARVLFLQQNAFVPEDASFAPELQKLYLLQL
                     RECDRIVREALSRGVLFDQASVLPLRSDLLSLRSLEKEEMAGRGEKWRNRLKAELEAI
                     EVMPQ"
     assembly_gap    721..730
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<731..1969)
                     /locus_tag="P5219_01050"
     CDS             complement(<731..1969)
                     /locus_tag="P5219_01050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRX25374.1"
                     /translation="MEKRGKVAGVSGPVVTVIPEKPAAMFEVARVGEEGLLGEVIRIE
                     RSCVRVQVYEDTDGIASGAPVVFEDDLLSVDLGPGLLGGVFDGIGRPLDLLGEEGIFL
                     PKGAKAGRRKKGVTFPFEPAAAPGDILSPGDLLGTVRENPTFLHSVMVPTDQLPGKAA
                     WIASAGGYAPEETIARLEDGREYSMIQKWRVRVPRPVRKRLPFEIPLLTGQRILDTLF
                     PVPLGGAAVLPGGFGTGKTVTQQSLAKWSSADLIIYIGCGERGNEMTEVLEEFPGLVD
                     PVSGGPLMDRTILVANTSNMPVAAREASIYLGMTLGEYFRDMGCNVAIMADSTSRWAE
                     ALREIGGRLEEMPGEEGYPAYLGSRLAQYYERAGRAEALGSPSRNGSITVVNAVSPAG
                     GDFSEPVTQASLRMSGAFWAL"
     gene            complement(1978..2244)
                     /locus_tag="P5219_01055"
     CDS             complement(1978..2244)
                     /locus_tag="P5219_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25375.1"
                     /translation="MTETGRGFAVGSQVFGDLWSLLGLVPLACESPGESASVFAALPE
                     EKAAFVAVEKEWFDGLPESVRKRLEKSADPVWIQFPSCESGEMR"
     gene            complement(2241..3302)
                     /locus_tag="P5219_01060"
     CDS             complement(2241..3302)
                     /locus_tag="P5219_01060"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HRX25376.1"
                     /translation="MIPSQKFIGESPNYEYLNAVIRARGTRLLGREVFSRLASGSLED
                     MELFLLESRYGPRYREQLATGGGSLLGRIENALAWGAADILRETASLARGESLLFFSV
                     ILSLGDLHNGRILLRASPGGIRPKNSPAWHRYSLADLAFYDDLWKKSATPADGAVRCH
                     EENHDFARILGESYRVLHETGDLFRAERALYSGWFGWWRERLRGRNNENSRRIEEYLG
                     RLTDLWNFSIWLRPRKEAVSRDQAFLPGGWGFSQEALESLDDPDKLFAGSGWSFVPGK
                     RGERTRSDLFRSFQRHFYEWQKSLYRQNLLGTDVSLGYAAQVVQEWKNLSLLAVGLSL
                     KMPAADLERHLFLQEEGTV"
     gene            complement(3259..3894)
                     /locus_tag="P5219_01065"
     CDS             complement(3259..3894)
                     /locus_tag="P5219_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25377.1"
                     /translation="MTGGKDLESFKEALAREHLEKTGRLERARDDEIADLIAERRVSV
                     QKKVTALRKEQDARFSFLAGEKRKSASASAGAFFLGEFSNLAASFGEDLHKMADTLRT
                     SDRERYGRALSRLAEEALMSCGRPAVLLVETGDADLLRETAENRSLFEGVEIRETHLN
                     GWGGCKAETENEIIDNTFLTRWERLSSWFSLRLSRCMHDSFTEIHRRIAEL"
     gene            complement(3910..4128)
                     /locus_tag="P5219_01070"
     CDS             complement(3910..4128)
                     /locus_tag="P5219_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HRX25378.1"
                     /translation="MEKALIALAAALAVGIPAVATAYAQAKIGSAGAGTVAEKPETGG
                     TIIILEAIPETMVILGFVVAIMLILQFA"
     gene            complement(4110..>4747)
                     /locus_tag="P5219_01075"
     CDS             complement(4110..>4747)
                     /locus_tag="P5219_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HRX25379.1"
                     /translation="FFGDAGHRLFHLEPLWVERSVSVLPVVVFTVALGAAHVFFGLLL
                     GIYQGVRNSHRHLWMEKAGNLAVLLALVAGLVVLKAEMPRGVFTVPFSLLVIGLALLI
                     AGGGIGGIIETLGSIGNIISYVRIAAIGLSSAILALVASKFLDVFGLSVFGIFLALMI
                     HLLNFVLAIGGSALHSARLHYVEFFGKFYSGNGKEYTPFQRRSGSSWKKHS"
BASE COUNT          983 a         1525 c         1319 g          910 t
ORIGIN      
        1 caagccacac cgtagcgctt ccgggggcaa gtcccatggt accctcgaat acctggatga
       61 ccgacaggtc tccgtcaatc tggaggatcc tgccggccct tcttcccgca cccgattcca
      121 ccgcgacgag ttccccgcag gcaggccgag aaattccccg cacgaagagc aggggaccgc
      181 ttatgccttc aattctgcgg aatccctcac ggtacagggc cgtcattggg gcatcacctc
      241 gatcgcttcc agttccgcct tcaggcggtt cctccatttt tctcccctgc cggccatctc
      301 ctctttttcc aggctcctca gggaaagaag atccgatctc aggggcagta cagacgcctg
      361 gtcgaagaga accccccgtg acagggcttc ccggacgatc cggtcgcact cccgcaactg
      421 gaggaggtac agtttctgga gttccggggc aaaggaagca tcctcaggaa caaaggcatt
      481 ctgctgcaaa aaaagcaccc tggccagctc ggccatgaac agggtccact tgtcctcctc
      541 ggagagaccg tcccggccca ccagctgcac cagttccagg agggactgtt ctctttcaag
      601 cagatccttg aggtagtccc gaagggcgaa ccattcctcc ccaaggttct cccggaacca
      661 cggggcgagc atcccatcgt agagggtata gctctggctc cagttgatcg ccggaaaatg
      721 nnnnnnnnnn gagggcccag aacgccccgg acatacggag gctcgcctgg gtcaccggct
      781 cagaaaaatc ccccccggcg ggagaaacgg cgttcaccac cgtgatggaa ccgtttcttg
      841 acggggagcc cagggcttca gccctcccgg cacgctcgta atactgggcg agcctcgatc
      901 cgaggtaggc gggatatccc tcttcgccgg gcatctcttc aagccggccc ccgatctccc
      961 tgagggcctc cgcccaccgg gaggtggaat ctgccatgat ggccacgttg cagcccatgt
     1021 cccggaaata ctcgcccagg gtcatgccga ggtagatgct tgcctccctg gctgccacgg
     1081 gcatattgga cgtgttggcc acgaggatcg tccggtccat gaggggaccg ccgctcacgg
     1141 gatccacaag cccggggaat tcttccagga cttcggtcat ctcgtttccc cgctcaccgc
     1201 acccgatgta gatgataagg tcggcggaac tccacttcgc cagggactgc tgggttaccg
     1261 tcttgcccgt gccgaagcct ccgggcagaa cagccgcacc tccaagggga acgggaaaga
     1321 gggtgtcgag aatgcgctgt cccgtcagca ggggaatttc aaagggaagg cgctttcgta
     1381 ccgggcgggg aacccggacc cgccatttct ggatcatgga atactcccgc ccgtcctcaa
     1441 gcctggcgat ggtctcctcc ggagcatagc cccccgccga agcgatccag gcggcctttc
     1501 ccggaagctg gtctgttgga accataacgg agtggaggaa ggtggggttc tccctgacgg
     1561 tgccgagaag atctcccggc gacaggatgt cccccggagc cgccgcgggt tcaaagggga
     1621 atgttacgcc ctttttcctc cggccggcct tcgctccctt cggaaggaaa atgccttcct
     1681 ctccaagaag gtcgagagga cggccgatgc cgtcgaaaac ccctcccagc agacccggcc
     1741 caagatccac tgacagcaga tcatcctcga acaccaccgg cgcccccgag gcgataccgt
     1801 ccgtatcctc gtatacctgc acccggacgc aggaccgttc aatgcggatc acttcgccga
     1861 gaagcccctc ttccccgacc ctcgcaactt cgaacatggc ggcaggcttt tccggaatca
     1921 ctgtcaccac gggtccggaa acacccgcca cctttcctct tttttccatg tgcgccgtca
     1981 cctcatctcc ccgctttcac aggaagggaa ctggatccat acaggatccg cagatttttc
     2041 cagccgtttt ctgaccgatt ccgggagccc gtcgaaccac tccttctcca ccgcgacgaa
     2101 ggcggccttc tcctccggca gggcggcaaa aacggatgcc gactctcccg gagattcgca
     2161 cgccagcggg accagcccca ggagggacca gaggtcgccg aacacctggc ttcccacggc
     2221 gaatcctctt cccgtttcag tcatactgtc ccctcctcct gcagaaaaag gtgccgttcc
     2281 aaatccgccg ccggcatttt aagggacagc ccgaccgcga gcagggagag gttcttccat
     2341 tcctgcacta cctgggctgc ataccccaaa gacacgtccg tcccgagaag attttgcctg
     2401 taaaggctct tctgccattc gtaaaaatgc cgctggaagg accggaaaag atcggaccgg
     2461 gttctttctc cgcgttttcc cggtacgaaa gaccatcctg acccggcaaa cagtttgtcc
     2521 gggtcatcca gggattcgag ggcttcctgc gaaaaacccc accctccggg aagaaaggcc
     2581 tggtccctcg acaccgcttc tttccttggc ctgagccaga tgctgaaatt ccacaggtca
     2641 gtcagcctgc cgagatactc ctcgattctc cggctgtttt cgttgttcct tcccctgagc
     2701 cgttcgcgcc accagccgaa ccagccggaa tagagcgcac gctcggcccg gaaaagatcg
     2761 cccgtttcat gaagcacccg gtaggactcc cccaggattc gggcaaagtc atggttttcc
     2821 tcgtggcatc ggactgcccc gtcggcgggg gtggcacttt ttttccacag gtcgtcgtaa
     2881 aaagcaagat cggcaaggga atacctgtgc caggcggggg aattcttcgg ccgaattcct
     2941 cctggagacg cccgcaggag aatccggccg ttgtgaaggt cgcccaggga gaggatcacg
     3001 gaaaaaaaaa ggaggctttc cccccgtgcg agagaagccg tttcccgcaa tatgtccgcc
     3061 gctccccagg ccagggcgtt ctctatcctt ccaaggagag atccgccgcc tgtggcgagc
     3121 tgctcgcggt acctgggccc atagcggctt tcaagcagaa aaagctccat atcctcgagg
     3181 gaaccggagg caaggcggga aaagacttcc ctgccgagaa gcctcgttcc tcttgcccgg
     3241 atcacggcgt tgagatattc atagttcggc gattcgccga tgaatttctg tgaaggaatc
     3301 atgcatacac cgggagagcc tgagcgaaaa ccaggaagac agtctttccc accgagtgag
     3361 gaaggtattg tcgataattt cgttttccgt ctccgccttg cagccgcccc atccgttcag
     3421 atgtgtttcc cgtatttcca cgccctcgaa aagacttctg ttttctgccg tttcccggag
     3481 aagatcagcg tctcccgtct ccacaagaag aaccgcgggc ctgccgcagg acatcagagc
     3541 ctcctccgca agtctggaaa gggcccggcc gtaccgctcc cggtcggaag tgcggagagt
     3601 atccgccatc ttgtgaagat cctctccgaa ggatgcggcc aggttcgaaa actccccgag
     3661 aaagaaggct cccgcggaag cggaggcgct tttcctcttc tcgccggcca gaaaactgaa
     3721 ccgggcatcc tgttccttcc gaagagcggt caccttcttc tgaacggaaa ctctcctttc
     3781 ggcgataaga tcggcgatct catcgtcccg ggctctttcc agacggcccg ttttctcaag
     3841 gtgctccctt gcgagagcct ccttgaagga ctcgaggtcc ttcccgccgg tcatttcctg
     3901 cccggtccct caggcgaact ggagaatgag catgatggcc accacaaagc cgaggatgac
     3961 catggtctcg gggatggctt caaggatgat gatggtgcct cccgtctccg gtttttctgc
     4021 caccgttccc gcgcccgcgc ttccgatttt tgcctgggcg taggcggtgg cgacggcagg
     4081 aattccgacc gcgagggccg ctgcaagggc tatgagtgct ttttccacga agatccgctc
     4141 cttcgctgaa atggtgtgta ttcttttccg ttcccggaat agaacttgcc gaagaactcc
     4201 acgtagtgca gcctggccga atggagggcc gagcccccta tggccagcac aaaattcaga
     4261 aggtgtatca tgagagccag aaagatgccg aagactgaca ggccgaagac atcaaggaat
     4321 ttcgaggcca cgagggccag aatagcggag gaaagaccaa tggccgcaat gcggacatag
     4381 ctgatgatat tgccgatgct gcccagggtc tcgatgatcc cgccgatgcc tcctcccgcg
     4441 atgagaaggg caagaccaat gaccagcaga gaaaagggaa cggtgaaaac tcccctgggc
     4501 atctccgcct tcaggacgac gagtcccgcg acgagtgcca ggagcacggc cagattgcct
     4561 gccttttcca tccagaggtg ccggtggctg ttcctgaccc cctggtatat gcccaggaga
     4621 agtccgaaaa agacgtgggc cgccccgagg gccactgtaa aaaccaccac cgggaggacg
     4681 ctcaccgaac gctccaccca aaggggttcg agatggaaga gcctgtgccc cgcgtcgccg
     4741 aagaatt
//
LOCUS       DAOTHD010000032         4722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_21524_length_4722_cov_3.056353, whole genome shotgun sequence.
ACCESSION   DAOTHD010000032 DAOTHD010000000
VERSION     DAOTHD010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21524_length_4722_cov_3.056353"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..976
                     /locus_tag="P5219_01080"
     CDS             14..976
                     /locus_tag="P5219_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRX25380.1"
                     /translation="MVLALGLFCGSAFAKAEIKLSNEFPPSHHVSKGLIVFAEKVAEH
                     SKGELTVKIFDSAQLFKDTEIVEALQDALIEVGLVPVNKWSGMIPAADVFEMPFVFKD
                     LESPKKFLAAGADKILDEEFQKKGVKNLFWVDYGYIQFFNNKHPLKRPEDFSGLTMRT
                     FSSGDAETLKALGAAPTVMSSSEMYMALQRGTVDGATTGMPAAVSRKVHEVQKYMTMA
                     NYTTAQFVVQGNLDWWNGLSAELKDAVLKAGLDAEEWIRGAIAESESAAEKVIREAGV
                     EIYSPTAEEREAFVKATEPVRKAFAEKTGEVGQKLIDMAISTSK"
     gene            1066..1581
                     /locus_tag="P5219_01085"
     CDS             1066..1581
                     /locus_tag="P5219_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRX25381.1"
                     /translation="MFKIIRSTVEKGNLILGYVSGLGILLMGLMLFYEVVCRYFFRSP
                     TIWAQEMSIYLFMWTMLAGGAYTLQQGKHVRIDLVIEHLSLRTQHFLEMITSVAGMVF
                     CAVITWQAYEMIAASIGYNKVSATLLRVPMWIPQMALLLGFALLTLQFAIIIIDRFHS
                     VFGNHEEGQSC"
     gene            1575..2855
                     /locus_tag="P5219_01090"
     CDS             1575..2855
                     /locus_tag="P5219_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRX25382.1"
                     /translation="MLSFSMVVFLLIGILFMGLPVAFSLGGTSLLLIALFDLPMKIVG
                     ATIFTSLDSFTILSIPLFVLMSQILLDGRIGDDLFDVMNAWVRHLPGGLAIATILACA
                     FFAAITGSGAATAATIGMVAYPAMIERGYDKKFTLGLLAAGGTLGILIPPSIPLILYG
                     SITEESVGKLFIAGVIPGLVLTAFFIVYAVIKSKRGGFTPMEKTSWGERIRVTRKNFW
                     GILLPFIIIGGIYSGAFTPTEAAAVGLVYGLFITLVVYRTLKLSDIPAICLRSVTTSC
                     MIAIIIAGAVLFGRVMTLLMIPQKLTELIISNNFSVLMFIIAMNVLMMILGCVLETVS
                     IVLLTMPLVTPILHALGIDPIWYAIVVTVNMTLALITPPVGMNLYVINSLRKDIRMGD
                     IISGVFPFLLIMIFFLILVIAFPGMSTWLPAVMH"
     gene            2941..3858
                     /locus_tag="P5219_01095"
     CDS             2941..3858
                     /locus_tag="P5219_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HRX25383.1"
                     /translation="MKTSKQLRNLLQRPGAVVVPGVYDSLSARICEMAGFEAVFHSGY
                     GTAAARLGQPDIGLLTLTEMAGQVKSITRAVRIPVLGDSDNGFGNAVNADRTVREYIW
                     AGAAGLFMEDQVSPKRCGHMEGKQVISRDEMMGKLRAAIDARNELDPDVVIMYRTDAI
                     HVTGFEDALDRAKAAVDAGVDMVFVEALETREQMERAVKEIGVPLMLNLIEGGKTPLV
                     PVKEAEALGFKMIVPALSALFAAARGMYEVMSEIRKEGVSEGYSDRLFTFREFAELVR
                     LDEIREMEEKYIPPSALEEKYRGKKRIVE"
     gene            4012..>4722
                     /locus_tag="P5219_01100"
     CDS             4012..>4722
                     /locus_tag="P5219_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRX25384.1"
                     /translation="MINQLKRKLAGGGVALGTFITLNCPDLVEIAALAGFDYCIIDTE
                     HGPGNPESIQHMIRAAELRGMAPIVRVTDTAATTILRTLDVGAAGIQVPQVNSPETAE
                     NVVRSAKYFPKGDRGACLTRSSRYGFVPGVAEYFDEANRETLVIVHCENRQGLECLDG
                     IAAVDGVDVVFVGPYDLSQSFGIPGQIYHPVMVDAVARALAAAKRAGKPAGIFVGSVE
                     EAKARIEQGFSYIAYSTDS"
BASE COUNT          949 a         1428 c         1369 g          976 t
ORIGIN      
        1 agcagcagcg gcaatggttc ttgccctcgg gctcttctgc ggttccgcgt tcgcgaaggc
       61 cgaaatcaag ctttcgaacg agttcccccc gtcccatcat gtttccaagg gcctcatcgt
      121 gtttgcggag aaagtggccg agcattccaa gggcgagctt acggtgaaga ttttcgactc
      181 cgcccagctt ttcaaggata ccgagatcgt ggaagccctc caggacgccc tgatcgaagt
      241 cggccttgtc ccggtgaaca agtggtccgg catgattccc gccgccgacg tcttcgagat
      301 gcccttcgtc ttcaaggatc ttgagtctcc caagaagttc cttgcggccg gcgccgacaa
      361 gattcttgac gaagagttcc agaagaaggg cgtcaagaac cttttctggg ttgactacgg
      421 ctacatccag ttcttcaaca acaagcaccc cctgaagaga cccgaggact tcagcggtct
      481 gaccatgagg acgttcagca gcggcgacgc agagaccctg aaggctctcg gcgcggctcc
      541 caccgtcatg agctcctccg aaatgtacat ggccctgcag aggggcaccg tggacggagc
      601 caccacaggc atgccggcgg cagtttcccg gaaggtccac gaggtccaga aatacatgac
      661 catggcgaac tacaccaccg cccagtttgt cgtccaggga aacctcgact ggtggaacgg
      721 tctctccgcc gagctcaagg atgccgtcct caaggccgga cttgatgccg aggaatggat
      781 ccgcggagcc atcgccgagt ctgagagcgc ggcagaaaag gttatccgcg aagccggtgt
      841 tgaaatctac tcccccacgg cggaagagcg ggaagccttc gtgaaggcca ccgagcccgt
      901 gcgcaaggcc ttcgctgaaa agaccggaga agtcggccag aagctcatcg atatggccat
      961 ctcaacctcc aaatagaccc ttccgggtag aggatatcct gtgcgaagcg ggaggggagt
     1021 cccttccgct tcttttctca aatacgagca aaggggcagt cttgcatgtt caaaattatt
     1081 cgcagcaccg tggaaaaggg caacctgatt ctcgggtacg tcagcggtct cggcattctc
     1141 ctcatggggc tcatgctctt ctacgaggtg gtgtgccgct atttcttccg gtcacccacc
     1201 atctgggccc aggaaatgtc gatttacctt tttatgtgga ccatgctcgc cgggggagcc
     1261 tacaccctcc agcagggaaa gcacgtccgg atcgaccttg ttatcgagca tctttccctt
     1321 cgcacccagc acttcctgga gatgatcacc agcgtggcgg gaatggtctt ctgcgcggtc
     1381 atcacatggc aggcctacga gatgatcgcg gcctccatag gctataacaa ggtttcggcg
     1441 acccttctcc gggtgcccat gtggattccc cagatggccc tccttctcgg tttcgccctt
     1501 ctgaccctcc agttcgccat tatcatcatc gaccgtttcc attccgtatt cggaaaccat
     1561 gaggagggac agtcatgctg agcttctcca tggtagtatt cctgctcatc ggcattcttt
     1621 tcatgggcct tccagtggcc ttttccctgg gtgggacttc cctcctgctc atcgctctct
     1681 ttgaccttcc catgaaaatc gtcggggcga ccatcttcac ctccctggac agcttcacca
     1741 tcctctccat ccccctcttt gtccttatga gccagattct cctcgacggg cggatcggag
     1801 acgacctttt cgacgtcatg aacgcctggg tgcggcatct tcccggaggg ctcgccatcg
     1861 ccaccatcct cgcctgcgcc ttcttcgccg ccataaccgg ttcaggtgcc gccaccgccg
     1921 ccaccatcgg catggtggcc tacccggcca tgatcgagcg cggatatgac aaaaaattca
     1981 ccctcggcct tctcgcggcg ggaggaaccc tcggtattct cataccgccc agcattcccc
     2041 tgatcctcta cggctccatc acggaagaat ccgtgggaaa gctcttcatc gccggagtta
     2101 ttccgggact ggtgctcact gcctttttta tcgtctatgc ggtaatcaag agcaaacgcg
     2161 gcggttttac tcccatggaa aaaacatcgt ggggggagag gatccgggtc accaggaaaa
     2221 acttctgggg cattcttctg cctttcatca tcatcggcgg catttactcg ggagccttta
     2281 cgccgaccga agctgcggcg gtgggacttg tgtacggact tttcatcacc ctggtggtgt
     2341 acaggacgct gaagctcagt gacattcccg ccatctgcct gcggtccgtc accacctcct
     2401 gcatgatcgc catcatcatc gccggtgccg tgctcttcgg gagggtcatg accctgctga
     2461 tgattcccca gaagctcacg gagctcatca taagcaacaa cttctcggtc ctcatgttca
     2521 tcatcgccat gaacgtcctg atgatgatcc tgggatgcgt tcttgagacg gtgtccatcg
     2581 tgctgctcac catgcccctg gtcactccca ttctccatgc cctcggcatc gacccgatct
     2641 ggtacgccat tgtggtgacg gtgaacatga cgctggccct cataaccccg ccggtgggaa
     2701 tgaacctcta cgtcatcaac agcctcagga aggacatcag gatgggcgat atcatctccg
     2761 gagtgttccc tttcctcctg atcatgattt tcttcctcat actcgtcatc gcgttccccg
     2821 ggatgagcac ctggcttccc gcagtcatgc actgaggagc cgggcaggct tcgctttttt
     2881 cgtccgccgg cggagggttc cttcctccgc cggcgttctt ataatgaaag gagacgaccc
     2941 atgaagacgt cgaaacagct gagaaacctc ctccaacggc cgggtgccgt cgttgttccc
     3001 ggagtgtatg actcgctgag cgcccggatc tgcgagatgg ccggtttcga agcggtgttc
     3061 cattcggggt atggtacggc ggccgccagg ctcggacagc ccgacatcgg gctgctgacc
     3121 ctcaccgaaa tggccggcca ggtaaaatcc atcaccaggg cagttcgaat tcccgttctg
     3181 ggagactcag acaacggctt cggaaacgcc gtgaacgccg acaggactgt ccgggagtac
     3241 atctgggcag gagcggcggg cctcttcatg gaggaccagg tgtctccaaa gcgctgcggc
     3301 cacatggagg ggaagcaggt gatctcccgg gatgagatga tgggcaagct tcgggcagcc
     3361 atcgacgcca ggaatgaact tgatcccgac gtggtgataa tgtacaggac agacgccatt
     3421 cacgtcaccg gtttcgagga tgccctcgac cgggcgaagg cggcggtgga cgcaggtgtc
     3481 gacatggtct tcgtggaggc cctggagacc cgggagcaga tggagcgggc cgtgaaggaa
     3541 atcggtgtgc ccctcatgct gaacctcatc gaggggggaa agactcctct cgtccccgtg
     3601 aaggaagcgg aggccctggg cttcaagatg atcgtcccgg ccctgtcggc cctgttcgcc
     3661 gcggccaggg gcatgtacga ggtaatgtcg gagatccgga aagagggcgt ctccgagggg
     3721 tattccgaca ggctcttcac cttcagggaa ttcgccgaac tggtccggct ggacgaaatc
     3781 cgggaaatgg aggagaagta cattccgccg agcgctctgg aggaaaaata ccggggaaag
     3841 aaacggatag tcgagtaaaa cggtcgctgc tgaagacatc ctcccgcaag ccccggcggg
     3901 caccggtaac actgccggcc ggggctttct tcatctgacc cgggtgtatc aggccggaag
     3961 aagtatataa ttgaaaagct ctgagattat tacccaacgg agggatacca ggtgataaac
     4021 cagctgaagc ggaaacttgc cgggggtggg gtcgcgctcg ggaccttcat caccctcaac
     4081 tgccccgacc tcgtggaaat agcggccctc gcgggatttg actactgcat catcgacacg
     4141 gagcacggcc ccggaaatcc tgagtccatc cagcacatga tccgggccgc ggaactccgg
     4201 ggcatggccc ccatcgtccg ggtgaccgac acggccgcca ccaccatcct ccgtaccctt
     4261 gacgtggggg cggcgggaat ccaggtgccc caggtgaact caccggaaac ggcggagaat
     4321 gtggtgcggt ccgccaagta tttccccaag ggcgaccggg gggcgtgcct taccaggtcg
     4381 tcccggtacg gattcgtccc cggcgtggcg gagtacttcg atgaggccaa ccgggagacc
     4441 ctggtgatag tccactgcga aaacaggcag ggccttgaat gcctggacgg tatcgccgcc
     4501 gtggacgggg tggacgtcgt ttttgtcggt ccctacgacc tctcccagtc attcggcatt
     4561 ccggggcaga tttatcatcc cgtgatggtg gacgccgttg ccagggccct ggcggcggcg
     4621 aaaagggcag gaaagccggc ggggatcttc gtgggatcgg tggaggaggc aaaggcacgg
     4681 atcgaacagg ggttctccta catcgcctac agcacggaca gc
//
LOCUS       DAOTHD010000033         4707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_21620_length_4707_cov_3.040843, whole genome shotgun sequence.
ACCESSION   DAOTHD010000033 DAOTHD010000000
VERSION     DAOTHD010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21620_length_4707_cov_3.040843"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            475..1620
                     /locus_tag="P5219_01105"
     CDS             475..1620
                     /locus_tag="P5219_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25385.1"
                     /translation="MDEKGIIVCAEEIDEGLELQLWTRGKTPRLVIVNRAKNTKKMSP
                     LSWLEGQERKLSLKGAGGKPVHYPMDVLEEPVRRMLYRFAQDPVFKGLLWHSVLFLSD
                     LFHAPRSVFDKSEMALLPEGKRRCLWLADFTDGGGKGFFRPFFPLSEAEMAVFGGEPS
                     IPIPGGKSVADLKKTGITRKLASVCPERWYETPQTAAAAVLLGFSMFFEESGDFSSFL
                     WKAAEGGMVSRKALSESPADPSISRFAVKMAGYVRHWAALDSISMETVLDSFDTMKER
                     GFARKRRMQFPVGALGNVEYTVTLYSDGEGTLAVGCVPAQATDRHRGERVFTLPEEVY
                     DQALRDDAFGGAEDDFFTLSTLLQAKLYEGWHRRIRRFTDVFLSPGG"
     gene            1623..2567
                     /locus_tag="P5219_01110"
     CDS             1623..2567
                     /locus_tag="P5219_01110"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HRX25386.1"
                     /translation="MPGGGRIRIGTCSWADRGLVASGWYPPSARTAAARLAFYGAAFD
                     TVEVDSTFYAIPEQASAFQWAARTPPGFLFNVKAWGLFTWHSVKYETLPEWVRHEIPR
                     PESGRLDFQGLPRELRPVLWKRFTSALEPLRTMDRMGYLLFQLPPKASFSPVMMRYLE
                     RVAEVTPPFRTAVEVRNRSWMEKGNRESFLSLLKGANMAYVAVDEPELDWTVGRDFPV
                     TATWGAVARFHGRNREGWTKKGATVAEKFRYNYSDGELLSWEGEVRNAAERAGKVFLM
                     FNNCFRDYAVKNALRMKCLLGVACSPGEGVQGELSFEE"
     gene            3002..3634
                     /locus_tag="P5219_01115"
     CDS             3002..3634
                     /locus_tag="P5219_01115"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HRX25387.1"
                     /translation="MCMRFWVLFCLVFTMLFTGVSGLTPALGADEALLRGVVYDEGQK
                     GLPGVAVSVWDGKLSYKAVTDFDGNFTIRGLLPGKPFAIRWTPRGGDSVKVDALTFPL
                     EGDLLLSMDYGSVGRGNVYTVRLPSNPSTGYGWTALHQGVAVVRLKENTFHGEGETFP
                     ERARTGRPGTELWKYEGIAKGKTALIFGYRRPWEKGITSSRYHVLSMTVR"
     gene            3713..>4707
                     /locus_tag="P5219_01120"
     CDS             3713..>4707
                     /locus_tag="P5219_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HRX25388.1"
                     /translation="MKILRSVLLFFAVLFSALITSFPLEASSAVTLPAGDEFARFREN
                     PPAVRNVTDRPLGWRPSPLDLSHVRPSAALRRELDILAAGAGGAPVFPRTFDLRELGA
                     LSPVRNQDPYGTCWAFASLASLESTFLKAGKGAFDFSEWHLAYFAYVDEGSSLPAFTA
                     IAPGFGSDPIFDQGGSVFQAAALLARWTGAVAESTRPYQNVSPWPESSRPSASDRAAK
                     RLENVFLIQGEGLLDEEAVKYALMRWGAVSVRVVWDDGAYSGEDFSYYNVPETGGGHI
                     VTVVGWDDDFPAANFVSAPGRDGAWIVRNSWGTGWGEAGYFYLSYAEPTLGYPAV"
BASE COUNT          907 a         1313 c         1510 g          977 t
ORIGIN      
        1 ggaaaagtga tcgtacaaaa aagacagttt gtaggaagtg tttggagtgt tatgtcatcc
       61 tgaggccggt tttttggccg aaggatctca gtctggaggt tcttaacccc agatccttcg
      121 ctgcgctcag gatgacaaac aagggcaata tcggcccttt cttgactctg gtgtgtcatt
      181 ctgagggcga agcccgatcg cggagcatcc ccggagggga agaatctgca cttgaagtgg
      241 ctcggaatca aacccgaggt ccctcctccc cggaggggaa ggggttcttc ataaagggtt
      301 agagtcaaaa ccgagatcct tccggcgaga agccacgccg tcaggatgac agccgagggc
      361 aaagcccttc gctctctcgg gacgacataa tcgattgccc cggcccgccc ctttgcattc
      421 gccgcctgag ccgtcataat aggatgcatc agatggaagc agggagtgcg ctgaatggac
      481 gagaaaggca tcattgtgtg tgccgaggag atagacgagg ggctggagct tcagctctgg
      541 accaggggga agactccccg cctggtgatc gtcaaccggg cgaaaaacac gaagaagatg
      601 agccccctga gctggctgga ggggcaggag agaaaattgt ccctcaaggg agcgggaggc
      661 aagccggtcc attatcccat ggatgtcctg gaggagccgg tcaggaggat gctgtaccgt
      721 tttgcccagg accctgtgtt caaggggctg ctgtggcatt ccgtcctctt tctgtctgac
      781 ctgttccacg ctcccaggtc ggtgttcgac aaaagcgaga tggccctcct tcccgaaggg
      841 aagcggcggt gcctttggct ggctgatttt accgacggcg gcgggaaggg cttcttccgc
      901 cccttctttc ccctgtcgga ggcggaaatg gcggtcttcg gaggggagcc ctccatcccg
      961 attccgggag gaaagtcagt ggccgacctg aagaaaacgg ggataacgag gaagctcgcc
     1021 tccgtctgcc ccgaacggtg gtacgagacg ccccaaactg ccgccgcggc ggttcttctc
     1081 ggcttttcca tgtttttcga ggagagcggc gacttttcct cctttctgtg gaaggcggcg
     1141 gagggcggca tggtgtccag gaaggctctt tcggagagtc ccgcagaccc ttccatctcg
     1201 aggttcgccg tgaagatggc ggggtacgtg cgtcactggg ctgccctgga cagcatttcc
     1261 atggaaacgg ttctcgactc ctttgacacc atgaaggaga gggggtttgc ccggaagcgg
     1321 cggatgcagt tccccgtggg ggccctcgga aacgtcgagt atacggtcac cctctattct
     1381 gacggggagg ggactctggc cgtggggtgc gttcccgccc aggccaccga ccggcacagg
     1441 ggagaaaggg tgttcactct gcccgaggag gtttacgacc aggcgctgcg ggacgatgcc
     1501 ttcgggggag cggaggacga cttcttcacc ctgtccacgc tgctccaggc caagctgtac
     1561 gagggatggc accgcaggat caggcggttc accgacgttt ttctttcccc gggagggtag
     1621 ccatgccagg gggaggaagg atccggatcg gaacatgttc atgggctgac cgggggctcg
     1681 tcgccagcgg gtggtatcct ccttccgccc ggacggccgc cgcgaggctg gcgttttacg
     1741 gggccgcttt cgataccgtg gaggtggaca gcaccttcta cgccatcccc gagcaggcat
     1801 cggccttcca gtgggccgcc aggactcctc cgggcttttt gttcaacgtg aaggcctggg
     1861 ggctgttcac ctggcactcg gtgaagtacg agaccctccc cgagtgggtg cgccatgaaa
     1921 ttccccggcc ggagtcgggg cgcctcgatt tccagggctt gccccgggag ctccggcccg
     1981 ttctctggaa gcggtttacc tcagccctgg agcctctccg gaccatggac cgcatggggt
     2041 acctcctgtt ccagcttccc ccgaaggcct ccttttcgcc ggtgatgatg cgctacctcg
     2101 agcgggtggc ggaggtcacc cctcccttcc gcacggcggt ggaggtgcgg aacaggtcgt
     2161 ggatggagaa ggggaaccgg gagtccttcc tgtcgctgct gaagggggcg aacatggcct
     2221 acgtggccgt ggacgagccg gagctcgact ggaccgtggg gagggatttt cccgtgacgg
     2281 ctacctgggg cgccgtggcg cggtttcacg ggcggaacag ggagggctgg acgaaaaaag
     2341 gagccaccgt ggcggagaaa tttcgctaca attactccga cggggaactc ctctcctggg
     2401 agggggaggt caggaacgcc gcggagcggg caggcaaggt cttcctcatg ttcaacaact
     2461 gcttccggga ttatgcggtg aaaaacgccc tccggatgaa atgccttctc ggagtggcct
     2521 gttctccggg agagggagtc cagggagagc tttcttttga agaataaagc tgataataat
     2581 agaaaataag agcccgaggg cgggaatcgc caatatatct atcccatagg atgtagtcgg
     2641 tgttgatgaa atgccccccc aagggtagaa tactacaaac gggttatccg gacggagaga
     2701 acagaaacga gaaagcaagt caggaaaaac cgaaagaagc agaaacaagt ccaagacagc
     2761 ctgtaccatg ttccatcagc accggaagct gcacagaaaa gaaccgaaac acaggaaaca
     2821 tgttagggta tctgcagcaa tatcaaggag ttggttgttt gaccaagtgt cagcaccacc
     2881 gtcccggata gcccgtaagc aatcggaggg ggaatttccc cctccttttt ttattttcca
     2941 tccattcgga caaaaaagtg ctactattac actggaacac aatatccatc aaggggaggg
     3001 aatgtgcatg cgtttttggg ttcttttctg cctggtcttt acaatgcttt ttaccggggt
     3061 gtccgggcta actcctgcac ttggggccga cgaggccctg cttcggggag tcgtgtacga
     3121 cgaagggcag aagggactgc ccggcgtcgc ggtttccgtg tgggacggaa aactgtccta
     3181 caaggccgtt actgatttcg acggaaattt taccatccgg gggctccttc ccggaaaacc
     3241 ctttgccatc cggtggaccc cccggggcgg cgattcggtg aaggtggacg cactgacctt
     3301 tccgctggag ggagacctcc ttctctccat ggattacggt tccgtgggca gggggaacgt
     3361 ctacacggtg cgcctcccgt caaatccctc cacggggtac ggctggacgg cgctgcacca
     3421 gggcgttgcc gtggtgcggc tgaaggaaaa taccttccac ggggaagggg agactttccc
     3481 cgagagggca aggaccggac ggcccggcac ggaactgtgg aagtatgaag gcatcgcgaa
     3541 gggaaaaacg gccctcattt tcggttaccg gcgcccgtgg gagaagggta taacttcgtc
     3601 ccggtaccat gttctttcca tgacggtaag atagtttgct aagaaagtca taccgggcag
     3661 ccctccttcc gaaggctgcc tttttttatc ctgtaaaacc gggaggaaac cgatgaaaat
     3721 ccttcgatcc gttctgcttt tcttcgccgt tcttttttct gccctgatta cttcatttcc
     3781 cctggaggct tcctccgcag tcactctgcc tgccggggat gaattcgccc ggttccggga
     3841 gaacccgccc gcagtccgta atgtcactga tcgtcccctt ggctggcggc cttcgcccct
     3901 ggatctctcc catgtgcggc cttctgcggc cctcaggaga gagcttgaca tcctggcggc
     3961 aggggcggga ggggcgcctg tttttccgcg gaccttcgac ctgagggaac tgggcgccct
     4021 ttcccccgtg aggaaccagg acccctacgg tacgtgctgg gcctttgctt ctctggcgtc
     4081 cctcgagtcc accttcctga aggccgggaa gggggccttc gacttttccg agtggcacct
     4141 ggcctatttc gcctatgtgg acgagggctc ttccctgccc gctttcacgg cgatcgctcc
     4201 gggttttggt tcagacccca tcttcgacca gggagggagc gtgtttcagg cggcagccct
     4261 tcttgcccgg tggacggggg ccgtggctga atcaacccgt ccctaccaga acgtcagccc
     4321 gtggcctgaa tcgagcaggc ccagcgcttc cgacagggcg gcgaagcggc tggagaacgt
     4381 cttcctgatc cagggtgaag gattactgga cgaggaggcc gtcaaatacg ccctgatgcg
     4441 ctggggggcg gtgagtgtcc gggtggtctg ggatgacggc gcctattccg gagaggattt
     4501 ctcctactac aatgtcccgg aaactggggg cggccatatt gtgaccgtcg tcgggtggga
     4561 cgacgatttt ccggcggcga atttcgtttc cgctcccggc cgcgacgggg cgtggatcgt
     4621 tcggaacagc tgggggaccg gctgggggga agcggggtat ttctatcttt cctacgccga
     4681 gccgaccctg ggctatcccg cggtctt
//
LOCUS       DAOTHD010000034         4690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_21720_length_4690_cov_2.975836, whole genome shotgun sequence.
ACCESSION   DAOTHD010000034 DAOTHD010000000
VERSION     DAOTHD010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21720_length_4690_cov_2.975836"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1549)
                     /gene="hypF"
                     /locus_tag="P5219_01125"
     CDS             complement(<1..1549)
                     /gene="hypF"
                     /locus_tag="P5219_01125"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HRX25389.1"
                     /translation="MLSHRKILVSGIVQGVGFRPFCARLAERLGLAGSVRNTSGGVEI
                     ELTGEPEILDSFFQSLSAENPPASVITSAVVLENQPASDPGEKTFRILGSKKEEDQSV
                     LIPPDLAVCDDCTAEMRDPSDRRYRYPFINCTNCGPRYTIIRDLPYDRPKTAMAPFPM
                     CPSCSREYHDSSDRRFHAQPNACFDCGPSVWLTDSWGTVLCRGDEAVGKCSALLDEGR
                     IIAVKGIGGFHIACTPFSDEVVARLRRRKGRKDKPFAVMASSIEEAERMAHIPASARE
                     LMLSPRKPIVLCCRRAEYPLSDLVAPGQKTVGILLPYSPLHHLILEEKKALIMTSANF
                     SDEPIVSSNEKALSVLSGIADFFLLHDREIYMAIDDSVAAPLGRSYFLLRRARGYIPA
                     PMALHADSPVIFGAGAEMKSSFCVSKGDLLFPGQYLGDMKQRETAVYYGRAMKHFLAL
                     YELTPEYLVHDLHPQFLSTATALGNLPGFRGEKLAVQHHHAHLAACLLENGVRGPAIG
                     AIFDGTGY"
     gene            1623..2150
                     /locus_tag="P5219_01130"
     CDS             1623..2150
                     /locus_tag="P5219_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRX25390.1"
                     /translation="MSLLDFGLIHVYMGEGKGKTSSALGLVVRMAGCGGKTAFIQFMK
                     GWVYSEVRGLSFLPGVKLIQTGRPDYIYPGSIDPVDYAEAEKGLRAARQILQSGCYDL
                     VILDEINVALSFGLLPLGEVVDLLRQKPLHVEVVLTGRNPPKELIELADLVTDMAEIK
                     HPYRKGILARKGIDC"
     gene            2193..>3854
                     /locus_tag="P5219_01135"
     CDS             2193..>3854
                     /locus_tag="P5219_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX25391.1"
                     /translation="MAKRTVGKQLSLEQLRKKTDIGSLGFTTTEDLQPLQEFIGQKRA
                     VSAISFGLEVESKGYNIFVLGNPGSGRTTYSLERLKTAAAKRAAPDDWVYVYNFDDPG
                     QPLAINLPAGKGKALGAAFDDLLEELKVAISKAFEKSQYEDAKAQLVKEFQEEVNQLM
                     EEVKGWAAENEFSLKRTPQGFVNIPLTEEEGENGEKNKREIQQEEFEALSEEEQKTWQ
                     KKSEEVSQKTLDTLRKIRDLEKGLKERIVKLEAEICRNAITPYLQDVKEKFGTTEILC
                     SWIDDLTEDIIDNFSIFVAAAKDENADIDFTRYTVNVFVCNDPEHGSPVIWETNPTYY
                     NLSGKVEYESRQGYLYTDFRKIVPGAFQKANGGFLVLDAEKVLLNFMSWEALKRVLRT
                     GEANIENLGEQYGAVPVSSLRPQPLPIRFKVVMVGTPYLYALLQYYDPEFLKMFKIKA
                     DFDTDMLRTQETERQMAQFIATCLKRENGLPFDASGVAEVIDWSSRTADDQERISTEF
                     NKITEVIVESSAWAKADGALLVTGQHVRKAVEEKKFRSNLIQERIA"
     assembly_gap    3855..3864
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3865..4633
                     /locus_tag="P5219_01140"
     CDS             <3865..4633
                     /locus_tag="P5219_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent protease"
                     /protein_id="HRX25392.1"
                     /translation="RAYEDGVIRIDTSGEIVGQINGLSVVDLMDYRFGHPSRITANVY
                     MGQEGVVNIEREVKLTGPIHNKGLLILTSFLGRKYAQDMPLSLSARITFEQMYGGIEG
                     DSASSTELYCLLSALSGTPLKQGFAVTGSVDQFGNIQPIGGVNEKIEGFFQYCEHRGL
                     TGEQGVIIPAQNVRHLMLEEKVLEAVKAGSFSVFSVTTIDEGIELLTGVSSSTIHRKV
                     KNRLKKWMKEGARLKKKYKLEAEEDNSAEEDNNDGEE"
BASE COUNT         1210 a         1220 c         1314 g          936 t
ORIGIN      
        1 cgtatccggt accgtcaaaa atcgcaccta tggccgggcc cctgaccccg ttttccagaa
       61 ggcaggcggc gagatgggca tgatgatgct gtactgccag tttttctccc ctgaagccgg
      121 ggaggttccc gagagctgtt gcggtggaaa gaaactgggg atggagatca tggacaagat
      181 attcgggcgt gagttcatac agcgccagga aatgcttcat ggcccgcccg taatagaccg
      241 ccgtttctct ctgtttcatg tctccgagat attgtcccgg aaacagcaga tctcccttgg
      301 aaacgcaaaa agaagacttc atttccgcac ccgctccgaa gatgacggga gaatctgcat
      361 gaagagccat cggagccgga atataccctc tcgctctccg gagaaggaag tatgaccgtc
      421 ccaggggagc ggctacggaa tcatcgatgg ccatgtatat ctccctgtcg tggagcagga
      481 aaaagtcagc gataccggaa aggactgaaa gggctttctc gttcgaggaa acaataggct
      541 catctgaaaa attggcactg gtcatgatca gggctttctt ttcctcgagg atgagatggt
      601 ggagcggtga atagggaagg agaatcccca ctgttttctg tcccggagca acaaggtccg
      661 acaacgggta ctccgctctc cggcagcaga gaacaatggg ttttctcggg gaaagcatca
      721 attcccgggc acttgccggt atgtgcgcca ttctctcagc ctcttcgatg gaagaggcca
      781 tcacggcgaa aggtttgtcc tttctcccct ttctccgccg cagccgggcc acgacctcgt
      841 cactgaaggg agtgcatgcg atgtggaacc ccccgatacc tttgacggca attatcctgc
      901 cctcatcgag aagggcggag catttcccga cggcctcatc gccccggcag agaaccgttc
      961 cccagctgtc ggtcagccag accgagggtc cgcagtcaaa gcaggcatta ggctgcgcat
     1021 gaaacctccg gtcggaggag tcatgatatt cccggctgca ggaagggcac atgggaaaag
     1081 gggccatggc cgttttcggc ctgtcgtagg gaaggtcccg tatgattgtg tacctcggac
     1141 cacagttcgt gcagttgatg aaggggtacc tgtatcttct gtcggacgga tcacgcatct
     1201 cggcggtaca gtcgtcacag acggcgagat cgggcggaat aagtacggat tggtcctcct
     1261 cctttttgct cccgagaatc cggaacgtct tttcgcccgg gtctgacgcc ggttggttct
     1321 cgagaacgac tgccgaagtg atgactgaag cgggagggtt ttcggcagaa agagactgaa
     1381 aaaaactgtc caaaatctcc ggttcaccgg taagctctat ctccacacct ccggaagtgt
     1441 ttctgacgga accggcaagg ccaagccttt ctgccagccg agcgcaaaaa ggacgaaatc
     1501 cgacaccctg aacgatgccg gagacgagaa tctttctatg ggaaagcatt gacaaacctc
     1561 caatctgatt gcattgtaat atacaaagaa aaaggtgcat agtgggacgg ggtgatgaag
     1621 ctatgtctct tttggacttt ggtcttatcc atgtttatat gggcgaaggc aagggaaaaa
     1681 ccagcagcgc ccttgggctt gtggttcgaa tggccggatg cggcggaaaa accgctttca
     1741 tccagttcat gaaggggtgg gtgtacagcg aagtccgggg actctcgttt cttcccggag
     1801 taaaacttat tcagacggga aggccggatt atatttaccc cggaagcatc gaccctgtgg
     1861 attatgccga agcggaaaaa gggctccgtg cggcgcggca gatcctccag tcagggtgtt
     1921 atgacctggt gatccttgac gaaatcaatg ttgccctgtc ttttggactt cttccgcttg
     1981 gtgaagtggt tgatcttctc aggcaaaaac ctctccatgt ggaagttgtg ctcacaggaa
     2041 gaaatccccc caaggaactg attgaactcg ccgacctggt gacggacatg gccgagataa
     2101 aacaccctta caggaaggga atcctggcca gaaaaggaat cgactgctga aaaggcaact
     2161 gaatacatga catattccgg gaggatatct ctatggccaa acgaactgtc gggaaacagc
     2221 tttcacttga acagctccga aaaaaaaccg atatcgggtc tctcggcttc acgacaaccg
     2281 aggatcttca gcccctgcag gaatttatag gacagaaacg ggccgtttcc gccatttcct
     2341 tcggactcga agtggagagc aaggggtaca acatctttgt cctgggcaac ccgggcagcg
     2401 gcagaacgac ttattctctt gagcggctca agacggcggc ggccaaaagg gcagctccgg
     2461 acgactgggt ctatgtgtac aactttgacg atcccggaca gcccctggct ataaatctcc
     2521 ccgctggaaa gggaaaagcc ctcggcgcag cctttgatga ccttctcgaa gaactcaagg
     2581 tcgccatcag caaagccttc gagaagagcc agtacgaaga cgcgaaggcg cagctggtga
     2641 aagagttcca ggaagaggtc aaccagctta tggaagaagt gaagggctgg gcggccgaaa
     2701 atgaattctc cctgaaaagg actcctcagg gattcgtcaa cattcccctt accgaggaag
     2761 aaggcgaaaa cggggagaaa aacaagcgag aaatccagca ggaagagttc gaagccctct
     2821 ctgaagagga gcagaagacc tggcagaaga aatcagaaga agtctcccag aaaaccctgg
     2881 ataccctcag gaaaatccgc gacctggaaa aaggcctcaa ggagcgcatc gtcaagttgg
     2941 aggcggaaat ctgcagaaac gccataacac cctatctcca ggacgtaaag gagaaattcg
     3001 gaacaaccga gattctgtgt tcatggatcg acgacctgac cgaagacatc atagacaact
     3061 tcagcatctt cgtcgcggcc gcaaaagacg aaaacgccga catagacttc acccggtaca
     3121 cggtgaacgt cttcgtgtgc aacgatccgg aacacggttc tcccgtcata tgggagacaa
     3181 atccgacata ttacaatctt tccgggaagg ttgaatatga aagccgccag ggatacctgt
     3241 acaccgattt cagaaagatt gtccccggag cgtttcagaa ggccaacggc gggtttctcg
     3301 ttcttgatgc ggagaaagtc cttctcaact tcatgtcctg ggaagccctc aagagggttc
     3361 tcaggaccgg ggaggcaaat atcgaaaacc ttggggagca gtacggagcc gttcctgtct
     3421 cttccctcag gccccagccc ctccccatcc gtttcaaggt cgtcatggtg gggacgccat
     3481 acctgtatgc cctgcttcaa tactacgatc ccgagttcct gaaaatgttc aagatcaagg
     3541 cagacttcga tacggacatg ctgaggactc aagagacgga acggcagatg gcccagttta
     3601 tagccacctg cctgaagcgg gagaacggcc ttcccttcga cgcttccggg gtggcggagg
     3661 tcatcgactg gtcgagcagg acagccgatg accaggaaag aatatccacg gagttcaaca
     3721 aaataacaga agtcatcgtc gaatcatcag cgtgggcaaa agcggacgga gccctcctcg
     3781 tcaccggtca gcacgtgcga aaagccgtgg aggagaagaa gttccgttcg aacctgatcc
     3841 aggaacggat cgcgnnnnnn nnnngagggc ttacgaggac ggggtcatca ggatagacac
     3901 ctccggagaa atcgtgggcc agatcaacgg cctctccgtt gtggatctca tggactaccg
     3961 cttcggccat ccttcaagga ttaccgcgaa cgtctacatg gggcaggaag gcgttgtcaa
     4021 catcgagcgg gaagtgaagc tcacaggacc tatccacaac aagggactgc ttatcctgac
     4081 aagcttcctc ggacggaaat atgcccagga catgcccctc tccctttcgg cccgcatcac
     4141 cttcgaacag atgtacggag gcatcgaggg agacagcgcc tcttccacgg agctgtactg
     4201 ccttctttcc gccctttccg ggacccccct gaagcaggga ttcgctgtaa ccggatcggt
     4261 ggaccagttc ggcaacattc agcccatcgg cggagtcaac gagaagatag agggcttttt
     4321 ccagtactgc gaacaccggg gcctcactgg ggagcagggc gttatcattc cggcccagaa
     4381 cgtccgccat ctcatgctcg aggaaaaagt cctggaagcg gtcaaggccg gaagtttttc
     4441 ggttttctcc gtaacgacca tcgacgaggg gatcgagctt ctgacgggtg tttcatcatc
     4501 cacgatccac aggaaggtga aaaacaggct gaagaaatgg atgaaggaag gggccagact
     4561 caagaaaaaa tacaagctcg aggctgaaga agacaacagc gcggaggaag acaacaacga
     4621 tggcgaagag tagtttttcc cgtcctgctg caatcgacaa ggtattcgac ggcctggaaa
     4681 aaatgtgggg
//
LOCUS       DAOTHD010000035         4677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_21789_length_4677_cov_3.430766, whole genome shotgun sequence.
ACCESSION   DAOTHD010000035 DAOTHD010000000
VERSION     DAOTHD010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21789_length_4677_cov_3.430766"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..351
                     /locus_tag="P5219_01145"
     CDS             133..351
                     /locus_tag="P5219_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRX25393.1"
                     /translation="MPKKFEVQVNNSWCKGCSLCIHICPKKVLELDEVRGKSVPARQD
                     DCIGCRQCENICPDMAITVKEKEEEKDA"
     gene            344..1477
                     /locus_tag="P5219_01150"
     CDS             344..1477
                     /locus_tag="P5219_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRX25394.1"
                     /translation="MPDVAFWQGNEAIAYGSLAAGCRFFGGYPITPSSEIAEIMAEEL
                     PKLNGRFIQMEDEIAGIAATIGGSIAGMKSLTATSGPGFSLKQENLGLAYMAEIPIVV
                     VDVMRGGPSTGLPTKTAQQDVMQARWGTHGDHGTICYAPASVQECYEVAIKAFNAAER
                     YRQPVLIMSDEIIGHMREKVLVPKIDTSKLVDRKRPADIPEKFVPYRPDPKDDLPCMA
                     SFGDGYTWHITGLTHNDWGFPTNNADEIEKKMKRLMRKIDRFRDDIVEYETVSAEDAE
                     VIVLAYGSVARSSLRAVHEARNRGIKAGFFRPITLWPFPDKELEKLARKVKTIIVPEL
                     NCGQMVLEVERAVHGKAKVVAHNLVNGELFKPEEILAVIEEVA"
     gene            1480..2298
                     /locus_tag="P5219_01155"
     CDS             1480..2298
                     /locus_tag="P5219_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HRX25395.1"
                     /translation="MPNQEVFSWLRTRFFPHIWCPGCGHGIIMHALLRALVTLKKKKS
                     QTVIASGIGCSSRMAGYIDACTVHTTHGRSLAFATGIKLAKPDLTVIDVMGDGDCTAI
                     GGNHFIHACRRNIDITAIVMNNNIYGMTGGQASPTTPAGSMATTTPYGAIDPSFDICK
                     LAEGAGAAYVARSTIANPKQAEQFILNGIQKKGFSVVEILTHCHTTFGRKNNRRTPID
                     NVNYFKNNSVPLAKAKDMSPEELAGKIITGEFVNKDVPEYTDQYLALIENVRGK"
     gene            2300..2863
                     /locus_tag="P5219_01160"
     CDS             2300..2863
                     /locus_tag="P5219_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRX25396.1"
                     /translation="MAGRYEIRFAGSGGQGVILAAVIAGEAASVFEDGLHVVQSQSYG
                     PEARGGKSKAEVIISTEPIDYPKAIKPNLQVILTQQAAEEYAGETLPGGRIIYDDFFV
                     TSFPQVDANVYYLPIVRTAREKIGKELVTNMVALGAVARVLELEKIAKPESIKKAILG
                     KVPEGTKQLNSQAFDEGYLLFKNSVHL"
     gene            2955..4325
                     /gene="rlmD"
                     /locus_tag="P5219_01165"
     CDS             2955..4325
                     /gene="rlmD"
                     /locus_tag="P5219_01165"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRX25397.1"
                     /translation="MAVKLGERVVLRTEKISSDGSCIAHTSEGLVVFVPGALPGEEIK
                     GKIVRIKREYAIAEPEEILSPHPGRNTPFCPLYERCGGCQLQHAEYSLQCRLKTDIVR
                     DAFERIYRGPFPEIPTCEPSPIERYYRNKGSLPIGSAEGGTAAGYYARRSHDIIPVAS
                     CPILAKEIGNLPGTVSGILTGLGFPPYNEKTGKGLFRHLILRSGTGTGNVLLSLVAAR
                     RLSKSEKERAEHRLIPLLRSQCQDLRTVTLNYNFSRSNVIIGKTTETLFGDGLIEERL
                     SPYRFRFDSTAFFQVNTEQAARLYKTAAQMAGQEGTENVLELYSGIGTLTCFLAEGAS
                     SVTAVEEWMPSVERMKDNVEANGLSGKIRILPGPVEDNISSLSENFDTVVVDPPRTGC
                     SREAIRYITTIAPRNIVYISCNPATLARDGALLREGGYFPLEIKCFDMFPHTVHVETA
                     VRFARR"
     gene            4599..4674
                     /locus_tag="P5219_01170"
     tRNA            4599..4674
                     /locus_tag="P5219_01170"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4632..4634,aa:Gly,seq:ccc)
BASE COUNT         1199 a         1292 c         1215 g          971 t
ORIGIN      
        1 agataaatat gttttcacag ttgaagtccc atcagcttaa agatattctt tcactaataa
       61 agcttaattg ccggctcatt ttccagctct gtttttgaac cagtttggac aatcccgtag
      121 gaggtgactt tgttgcccaa aaagttcgaa gtccaggtga acaattcgtg gtgtaagggc
      181 tgttccttgt gtatccacat ttgcccgaaa aaagtgctcg agctcgatga agtgcgcgga
      241 aagtctgtcc ctgcccgtca ggatgactgc atcggctgca gacagtgcga gaacatctgc
      301 cctgatatgg caattaccgt gaaagagaag gaggaagaga aggatgcctg atgttgcctt
      361 ctggcaggga aacgaggcaa tcgcttacgg ttctctcgcc gcaggatgcc gatttttcgg
      421 aggatatccc atcacgccct cttccgagat agctgaaatt atggctgaag aactcccgaa
      481 actgaacgga agatttatcc agatggagga cgagattgcc ggaatagccg ccacgatagg
      541 gggctccatc gccggaatga aatccctgac tgcgacttcc ggccccggat tctccctcaa
      601 gcaggaaaac ctcggccttg catacatggc cgagataccc atagttgttg tggatgtaat
      661 gcgcggcggt ccctccacag gacttcccac gaaaaccgct cagcaggatg tcatgcaggc
      721 ccgctgggga acccacggtg accacggcac catctgttat gctcccgctt ccgtccagga
      781 atgctacgag gttgccatca aggcgttcaa cgccgctgag agataccgtc agccggttct
      841 gatcatgagc gatgaaatca tcggccacat gcgcgaaaaa gtcctcgtgc cgaaaattga
      901 cacttcaaag ctcgtggacc gcaagcgccc cgcagatatt cctgaaaagt tcgttcccta
      961 ccgcccggat ccgaaagacg atcttccctg catggcatcc ttcggtgacg gctacacgtg
     1021 gcatattacc ggcctgaccc acaacgactg gggatttccc acgaacaacg ccgatgaaat
     1081 cgagaaaaaa atgaaacgtc tcatgagaaa aatcgaccgc ttccgcgatg acatcgtcga
     1141 atacgaaaca gtctccgccg aagatgcaga agtgatcgtt cttgcctacg gaagcgtggc
     1201 ccgctcaagc ctgagggcag ttcacgaggc gaggaacagg ggcatcaagg ccgggttttt
     1261 cagacccatc accctctggc ctttccccga caaggaactg gaaaagctcg cccgcaaggt
     1321 gaaaaccatt atcgttcccg agctgaactg cggccagatg gtcctcgaag tcgagcgggc
     1381 cgtccacggg aaggccaagg ttgtggctca caaccttgtc aacggcgaac ttttcaagcc
     1441 tgaagaaatt ctcgctgtca ttgaggaggt ggcctgatta tgcctaatca ggaagtgttc
     1501 agctggctgc gcacacgatt ttttccccat atctggtgtc ccggatgcgg gcacggcata
     1561 atcatgcatg ctctcctcag ggccctggtt accctcaaga aaaagaaaag ccagacggtc
     1621 atagcatccg gcatcggctg ttcgagccgc atggccggct atatcgatgc atgcacggtc
     1681 cacaccaccc acgggagatc acttgctttc gccacgggca tcaagctcgc caagccggat
     1741 cttaccgtta ttgacgtcat gggtgacgga gactgcaccg ctatcggggg caaccacttc
     1801 atacacgcct gcaggcggaa catcgacata acagccatcg tcatgaacaa caatatctat
     1861 ggaatgaccg gcggacaggc ctctccgacc acccccgcgg gaagcatggc caccaccacc
     1921 ccctacggcg ccatcgatcc ttcctttgac atctgcaaac tcgcggaggg agcgggtgca
     1981 gcctacgttg cccgttccac catcgccaac cccaagcagg ccgaacagtt catcctgaac
     2041 ggaatccaga agaagggatt ctccgtggtg gaaatactga cccactgcca taccacgttc
     2101 ggaaggaaaa ataaccggag gacccctatc gacaacgtca actacttcaa gaacaactcc
     2161 gttcccctcg ccaaggcgaa agacatgtcc ccggaggagc ttgccggaaa gatcatcacc
     2221 ggagaattcg tcaacaagga tgtccccgaa tatacggacc aatacctcgc cttgatcgaa
     2281 aacgtaaggg ggaagtgaaa tggcaggaag atatgaaatc cgatttgcgg gttccggcgg
     2341 ccagggcgta atccttgccg cagtcattgc cggagaggca gcgtccgttt tcgaggacgg
     2401 cctccatgta gtccagtccc agtcctacgg ccccgaagcc cgggggggga agtccaaggc
     2461 cgaggtgatc atctccaccg aacctatcga ctacccgaag gcgatcaagc cgaatctgca
     2521 ggtcatactg acccagcagg ccgcggagga atacgccgga gaaacacttc cgggaggcag
     2581 aatcatctac gatgatttct tcgttaccag cttcccccag gtggacgcca acgtatatta
     2641 ccttcccatc gtgcggaccg cccgtgaaaa aatcggcaaa gaactcgtca cgaacatggt
     2701 cgcccttgga gcggtcgcca gggttctcga acttgagaag atcgcaaagc ccgaatcgat
     2761 caagaaggct attctcggca aagtgcctga agggacgaaa cagctcaact cccaggcatt
     2821 cgatgaaggg taccttcttt tcaaaaacag cgttcacctg taaaattcac aggaacatct
     2881 tccgttaaag aggaaggcaa aggtcattac agtgaccttt gccttctgtt ttttggacac
     2941 actggattgg gggcatggct gtgaaactgg gtgaaagggt cgttctgcga actgaaaaga
     3001 tcagcagcga cggaagctgt atcgcacaca cttccgaagg acttgtggtg tttgtccccg
     3061 gggctctccc cggagaagag atcaagggaa aaatcgtcag gataaagaga gagtacgcca
     3121 tagcggaacc ggaagaaata ctttctcctc atccgggcag gaacactcct ttctgtcctc
     3181 tctatgaacg gtgcggagga tgccagctcc agcacgcaga gtattccctc cagtgcaggc
     3241 tgaagactga tattgtccgg gatgcttttg aaaggattta ccggggccca tttcccgaaa
     3301 tccccacctg tgaaccgagc cccatagagc gatattaccg caacaaagga tctcttccca
     3361 taggaagcgc ggagggggga acagctgcag ggtattacgc gagaagaagc catgacatta
     3421 ttcctgtcgc ttcgtgcccc attctcgcga aggaaatcgg aaatctgccg ggaacggttt
     3481 ccggcattct tactggcctt ggctttcctc cctacaacga aaagaccgga aagggactgt
     3541 tccgccatct tattctgaga agcggcaccg gtactggaaa cgttctcctc tcgcttgtcg
     3601 cagcccgaag gctctcgaaa tccgaaaaag aacgtgccga acacagactc ataccccttc
     3661 tcaggtccca gtgccaggac cttcggactg taacgctgaa ctacaatttt tccaggagca
     3721 atgtcatcat cggcaaaaca acagaaacac ttttcggtga cggactgatc gaagaaagac
     3781 tctcccccta caggttcaga tttgattcta ccgccttttt tcaggttaat acggagcagg
     3841 cagccagact ctacaaaact gcggcgcaaa tggccgggca ggaaggaacg gagaacgtcc
     3901 tcgaactcta cagcggcatc ggtaccctta cctgcttcct tgcggaagga gcatcttcgg
     3961 tgacggctgt ggaggagtgg atgccttcgg tggaaaggat gaaagataat gtggaagcca
     4021 acggcctgtc cggaaagata agaatcctcc ctggaccggt tgaagacaac atttcatccc
     4081 tttcggaaaa cttcgatacg gtcgttgtcg atccccccag gacagggtgc agccgggagg
     4141 caatccgtta catcacaacc atcgcaccca gaaacatcgt ctacatctcc tgcaacccgg
     4201 caaccctggc acgggacggc gctctcctca gggaaggggg atattttccc ctggaaatca
     4261 agtgttttga catgttccca cacactgttc acgtagaaac ggcggttcgc ttcgcccgca
     4321 ggtaatcagg gtttcacagg cttttcggag atgatgaaat tcatcgggaa aacagcacct
     4381 gctttcggaa gcgctttcat tgtcatatct ccttgaaatg tggggatgcc cctgccggag
     4441 gacaccggaa ttttgagcat tagccgttcc gtttttcggg gtgagaacag cagattgtac
     4501 aaaagtcagg atccggcaag aaaattcgag ccatctcgtg ccatccgtat tgccaaaaga
     4561 aaaagaggcg gatataatat ccttcgtctg acaagagagc ggacatagct cagctggtag
     4621 agcattagct tcccaagcta agggtcgcgg gttcgaatcc cgttgtccgc tccaatt
//
LOCUS       DAOTHD010000036         4666 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_21855_length_4666_cov_3.729777, whole genome shotgun sequence.
ACCESSION   DAOTHD010000036 DAOTHD010000000
VERSION     DAOTHD010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21855_length_4666_cov_3.729777"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..585
                     /locus_tag="P5219_01175"
     CDS             142..585
                     /locus_tag="P5219_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25398.1"
                     /translation="MRVRKLIFTAFLALICCFPFAAWAAETGTAPLPENARVVDGHMT
                     SAMKDGKPLDRLIAYTQESPAFYAAGTLLSAPPHTKIRFSWFFATDNRHITDVELNTE
                     NHGSDIFVYSTLNNGGDPWPKGAFRVEIFVNDRSDPDQIIDFEVE"
     gene            complement(650..1846)
                     /locus_tag="P5219_01180"
     CDS             complement(650..1846)
                     /locus_tag="P5219_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRX25399.1"
                     /translation="MNAESIRQRAELLSEKLTELRRKFHANPELPWQEKETSLFVEEY
                     LRNLGLENIRRGFGGTECGVAADLRGAKPGPCVALRGDMDALPLEEENDLPYRSTRPG
                     VMHACGHDAHTAILLGTAEILSSMKDELSGTVRFLFQPAEEAGYNSGAPRMIEEGALD
                     GVDAVGGLHVWSLLPAGKLGCRVGPVMASADIWDLKIQGKGGHGAMPHKAVDPTVTAA
                     TVISTLQTVVSREMDPQDTVVLSVGKLEAGTAVNIIPDTARVAGNVRTTSRDVRDRME
                     GIMKRVADGICAAMRCTAELTYTPIYPVTVNDPEVTAVMRSAAADILGEENIEEVPVA
                     MGSEDFSYYGEKVPSAYVFLGIADDAKGTGNQHHNPKFNVNDDVLPSGAALLAVFAVR
                     ITGRSS"
     gene            complement(1937..2308)
                     /locus_tag="P5219_01185"
     CDS             complement(1937..2308)
                     /locus_tag="P5219_01185"
                     /inference="COORDINATES: protein motif:HMM:NF013089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HRX25400.1"
                     /translation="MDRLTFFLLAGSAVFNASANTLMKLAFGHQKDLLDSGAVNAVLR
                     IVLNPWAVAGVGCFGISFVFLSAALTRTDLSLAYPFMAGLVFLLVLAVSVLFFSEQVT
                     LWRLAGVAAILAGIWIISVKG"
     gene            2462..4114
                     /locus_tag="P5219_01190"
     CDS             2462..4114
                     /locus_tag="P5219_01190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRX25401.1"
                     /translation="MNFSRPGTALKLLLLVLLSVTLYFWGLGSHGLLEPDEGRYSEIP
                     REMRETGDFITPKLNYIKYFEKPVLHYWLTAAAQSAFGENEFSGRFWPALLALGGILS
                     TFFLARSMFGRETALYSSGILATCLVYFAVSQINITDMPLSFFITVSMTGFWFGLEKN
                     RRFYLLFYAGAALALLTKGLIGIVLPGGIIFWYMIFTRRWDIVRSALYLPGIILFAAL
                     SLPWFIAVCLVNGDFFYFFFIQEHFLRYATKMHGRYEPAWFFIPILLLGLFPWTGLLP
                     GALRSAVPSPLRSFGKERREELFLFLWFAVIFLFFSLSSSKLIPYIIPVFPPLAVLMG
                     RLLHRTVSEENWKGMKRFLLWNSILLIPFILALLVYPFFNSRISAGKLLPYSLPVAAA
                     LSAFTTAGWYFFRKRRFRLLAVSLCLLSFLVMFSFKRVFSLYDGLLSARELAGAVAEL
                     RRPGDVIAQYGNYDQGLPFYLKQRIVLVNYLGELEFGAKQEQDPFWFIGEEQLKELWN
                     GDRRVILIINDGHMEKILALLDPHISSVALKTEKRVVLVNRR"
     gene            4209..>4666
                     /locus_tag="P5219_01195"
     CDS             4209..>4666
                     /locus_tag="P5219_01195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRX25402.1"
                     /translation="MQEPGLVSVVIPVYNEEECLPHLFPRLRAALEGMNRPHEIIFVN
                     DGSRDRSLQMLYEFYKANPKTVRIIDFNGNFGQHMAIMAGFDHSRGDIIITLDADLQN
                     PPEEIPRIVAKIDEGHDVVGTVRQNRQDPKFRKMASRIVNSITNRITGLCL"
BASE COUNT          910 a         1450 c         1249 g         1057 t
ORIGIN      
        1 gtcaagaacg ccagggtggt tgccgccttt tgaccgttgc ctggctgtgc atgatcattt
       61 tttatttttt cgccgcaatg ctgtgataga atccctccag gtagtcccgg gagggattct
      121 tcaattgagg aggaggaaaa aatgagagtt cggaaactga ttttcacggc gtttctggcg
      181 ctgatctgct gcttcccatt cgcggcatgg gccgcggaaa ccggtactgc gccgcttccc
      241 gaaaacgctc gggtagtaga cggacatatg acatccgcga tgaaggacgg gaaacctctt
      301 gacaggctca ttgcctacac ccaggagtcg ccggcgtttt atgccgcggg aacgctgctc
      361 tctgctccgc cccacacgaa aatccgtttt tcatggtttt tcgccacgga taaccggcat
      421 attaccgatg tggaactgaa caccgaaaat cacggttccg acatcttcgt ctacagcacc
      481 ctcaacaacg gtggggaccc atggccgaag ggcgcgttcc gggtggaaat atttgtcaac
      541 gacagatctg atcctgacca gattatcgat ttcgaggtcg agtaagacag cggcgaaaag
      601 acgggggctc ccggggaatt ccccgggagc ccccgtcttt tcatctcctt cagcttgatc
      661 gtcccgttat cctgacggcg aataccgcga ggagagcagc ccctgaggga aggacgtcgt
      721 cgttcacgtt gaattttgga ttgtggtgct ggttgcccgt ccctttcgcg tcgtcggcga
      781 tgccgaggaa cacgtaggct gacgggactt tttccccgta atagctgaaa tcctccgatc
      841 ccattgccac gggcacttcc tctatgtttt cctcccccag gatatcggcg gctgcggaac
      901 gcatcactgc cgtcacttcc ggatcgttca ccgtgaccgg gtaaataggg gtgtacgtca
      961 gttcggcggt acatctcatg gcagcgcaga tgccgtcggc taccctcttc atgatgcctt
     1021 ccatccggtc ccggacgtcc cggctggtgg tccgcacatt tccggccacc ctcgccgtat
     1081 cggggatgat gttgacagcc gttcccgctt ccagtttgcc cacgctgagc accaccgtat
     1141 cctggggatc catctccctg ctcaccacgg tctggagagt cgagatcacc gtagctgcgg
     1201 tcacggttgg gtctacagcc ttgtgtggca tagcgccgtg gccgcccttt ccctggatct
     1261 taagatccca gatgtcggcg gaggccatga cgggccccac ccggcagccg agtttccccg
     1321 ccggaagaag ggaccagacg tgcagtcccc ccacggcgtc aactccgtcc agggctcctt
     1381 cctcgatcat cctgggggcg cctgagttgt atcccgcttc ctccgccggc tggaagagaa
     1441 atcggaccgt gccggagagt tcgtccttca tggaggaaag aatctccgcc gtgcccagca
     1501 gaatggcggt atgggcgtcg tggccgcagg cgtgcatgac gccgggccgg gtagagcggt
     1561 agggaaggtc gttctcctcc tcaaggggaa gggcgtccat gtcgcctctc agggcaacgc
     1621 acggtccggg ctttgctccc cgcagatccg cagctacgcc gcattccgtc ccgccgaagc
     1681 ccctgcggat gttttcgagg cccagattcc ggaggtattc ctccacgaag agggatgtct
     1741 ctttttcctg ccagggaagt tcggggttcg catggaattt tctccggagt tccgtgagct
     1801 tttccgacag caattcagct ctttgcctga tcgactctgc attcatctgc taaattctct
     1861 cctttctcct tggtgcattc tcgttttcac tgcccgtata atgcgggggg aacctctgcc
     1921 ttcagcctgc tccgggtcag ccttttaccg aaatgatcca gattcccgcc agaatggccg
     1981 ccacgccggc aaggcgccag agggtgacct gttccgaaaa aaagagcacg gaaacggcca
     2041 ggaccagcag gaagaccaga cctgccatga atggataggc cagggagaga tcggtcctcg
     2101 tcagcgccgc gctgagaaag acgaaagaga tgccgaaaca gcccaccccg gccacggccc
     2161 agggattgag gacgatccgg agcaccgcgt tcaccgcccc gctgtcaagc agatccttct
     2221 gatggccgaa ggccagcttc atgagagtgt tcgccgaggc gttgaagacc gccgatccgg
     2281 cgaggagaaa aaatgtgagc ctgtccatgg acatacctcg ctttcgggtg cattgaagga
     2341 ttatactcca tgatgcttcc aaaaacggga agagttcctg cagaattctt ccacatcggg
     2401 aagggtatca tgtaaaattg taaaattcac ggtctcaact aatttccgga aggatggtac
     2461 gatgaatttc tcccgccccg gcaccgccct gaaactgctg cttctcgttc ttctgtccgt
     2521 cactctttac ttctggggcc tcggaagcca cggactcctc gagcccgacg agggacgcta
     2581 ctccgaaatc ccgagggaaa tgcgcgagac aggggatttc atcaccccga agctcaacta
     2641 catcaagtac ttcgagaagc cggtgcttca ttactggctg acagccgccg cccagtccgc
     2701 tttcggagag aacgagttct ccgggcggtt ctggcccgct ctcctcgccc ttggagggat
     2761 cctctccacg tttttcctcg ccagatccat gttcggccgg gaaacagccc tgtattcatc
     2821 gggcatcctg gccacatgcc tcgtttattt cgccgtttcc cagatcaaca tcaccgacat
     2881 gcccctctcg ttcttcatca cggtttccat gacggggttc tggttcgggc tggaaaaaaa
     2941 caggcggttc tacctgcttt tctacgcggg agcggccctc gccctcctta ccaagggcct
     3001 gatcggcatc gtcctccccg gcgggatcat tttctggtac atgatcttca ccaggcggtg
     3061 ggacattgtc cggtccgccc tctatcttcc cggcatcatt cttttcgccg ccctttccct
     3121 gccctggttc atcgcggtct gcctggtgaa cggcgatttc ttctattttt tctttatcca
     3181 ggaacatttt ctcaggtacg cgacaaagat gcacgggcgc tacgaacccg cctggttctt
     3241 tatccccatc ctgctgctcg gactcttccc ctggaccggc ctgctgccag gagccctgag
     3301 gtccgcagtc ccttcgccgc tccgttcctt cggaaaagaa cggcgggaag aacttttcct
     3361 gtttctctgg ttcgccgtta tctttctctt tttctccctg tcgagctcca agctcatccc
     3421 ctatatcatc cccgtcttcc cgcccctcgc ggtcctcatg gggaggctgc tccaccgcac
     3481 cgtgtcagaa gaaaactgga agggaatgaa aagatttctt ctctggaact ccattctcct
     3541 cattcccttc atcctggccc tgctggtgta ccccttcttc aacagccgca tctccgccgg
     3601 gaaacttctg ccttattccc ttccggtggc ggcggccctt tcagccttca ccacggccgg
     3661 atggtatttc ttccggaaaa ggcgattccg tctcctggcg gtttctctct gcctgctgtc
     3721 cttcctcgtc atgttttcat tcaagagggt gttttccctt tatgacggcc tgctctccgc
     3781 aagagagctc gccggagcgg tggcggaact ccgccgcccc ggcgacgtga tcgcccagta
     3841 cggcaactac gaccagggac ttcccttcta cctgaagcag cgcattgtcc tcgtcaatta
     3901 cctcggggag ctcgagttcg gggcgaaaca ggaacaggac cccttctggt tcatcggaga
     3961 ggagcagctc aaggaactct ggaacggaga caggagggtc atcctgatca tcaatgacgg
     4021 gcacatggaa aaaatcctgg ccctgcttga cccccacatc tcatccgttg ccctgaaaac
     4081 ggagaagagg gtcgtcctcg tcaaccgccg gtaaaacggc tgtcacccga tatttcggcg
     4141 gccctgccgc ctgacgattc aatcccgtac tgcggggatg cagtccccgg aaaaggagat
     4201 gtgccgccat gcaagaacca ggcctcgttt cagtggtcat accggtctac aacgaggaag
     4261 aatgccttcc ccatcttttt ccccgccttc gggcggccct tgagggaatg aaccgtcccc
     4321 atgaaatcat cttcgtgaac gacggcagca gggacaggtc cctccagatg ctctacgaat
     4381 tttacaaagc gaacccgaag acagtccgga tcatcgactt caacggaaat ttcggccagc
     4441 acatggccat catggccgga ttcgaccact cccgtgggga tatcatcatc accctcgacg
     4501 ccgaccttca gaaccccccg gaggaaatcc cccggatcgt ggcgaagatc gacgaggggc
     4561 acgacgtggt ggggacggtc aggcagaaca ggcaggaccc gaaattccgc aagatggcgt
     4621 ccaggatcgt caattccatc accaacagga tcaccggtct ctgtct
//
LOCUS       DAOTHD010000037         4593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_22326_length_4593_cov_2.626928, whole genome shotgun sequence.
ACCESSION   DAOTHD010000037 DAOTHD010000000
VERSION     DAOTHD010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22326_length_4593_cov_2.626928"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="P5219_01200"
     CDS             complement(<1..451)
                     /locus_tag="P5219_01200"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRX25403.1"
                     /translation="MNKKVRGIFAPIATAFDASGEVDYSTFAENTVAFGATKLSGLVV
                     LGSNGEFTLLSHEEKVKLVETARNHLPAEKMVIAGTGCESFRETLQLTKECAAVGADV
                     ALVVTPNYYKKDMNEAALGNFYTMLADASPIPVMIYNMPGNSGVNVPS"
     gene            complement(546..1829)
                     /locus_tag="P5219_01205"
     CDS             complement(546..1829)
                     /locus_tag="P5219_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRX25404.1"
                     /translation="MLTAVFGGMFLALLLSIPIAAAIGFSCLAFIPWIPGGLDRVMFF
                     VGQRMVVTADSFTLLALPFFILAGTLMSHGGIARKLTDLAEAVAGDFPGGLGIAAIVA
                     CMFFGAVSGSGPATVAAIGTIILPAMISRGYGVGFSGGLVACAGGIGVLIPPSVPMIV
                     YGVNSGVSITDLFIAGLFPGILVGLFLMIPAVYISRKKGYAGLPREGGTGWVLKRVWQ
                     AKSALLMPLIILGGIYSGVFTPTEAGIVAVAYAIVLGFATGGLTMKGLCSAMVEAAVI
                     TGSCIFLMGTASAFAKLLHMKDVPNLISNLIFSLTTNKFLILLMFNAVFLVGGMFIDT
                     LSNILIFCPIFLPLALKIGVDPIHFGTFVVANLALGMATPPMGVDLFVASNICKTPFE
                     KVLRGSIPFMVWNLAAVLMITYIPMLSLWPLQFFR"
     gene            complement(1843..2340)
                     /locus_tag="P5219_01210"
     CDS             complement(1843..2340)
                     /locus_tag="P5219_01210"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRX25405.1"
                     /translation="MKILSLADRYFEEALAVILFSAILLIGMEQVVSRYLLRFVHSWA
                     EELMRILFVALSLVSFSLCAKKRQHVKVEILTMSLPPKLGKAVSLFASLAFLGFTLLV
                     VKYSYNITRLQFESGQTTAAMDMPTWTYFSLGPVLFLLMAFRIVQKDILPVFFPREEQ
                     TETLK"
     gene            complement(2402..3418)
                     /locus_tag="P5219_01215"
     CDS             complement(2402..3418)
                     /locus_tag="P5219_01215"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRX25406.1"
                     /translation="MTTMKRLVFCVLLAALVLSFSAVSYAKEFVMGHTGQPLVTFAQA
                     GEKFAELLDKHSGGKMKVQVMGAAALGNNREGIEQIQQGVTDFWLISTGLLAPFTKAV
                     TVYDVPYLFKSEKAALDFVNSDLAMEVVKPLEEKGIKPLGYFIMGWRHTHSNIAVRKP
                     EDLKGVKIRTEPAPIRMAIFKAMGANPIPMDFGEVFTSLQQGVIDAGENTFENIKSQG
                     FHTVQKYITTDGHILDPMLIVMSKKSWDKLSDEEKAVLQKAVDETCTWEQDNVLANNK
                     KIMEEFKAAPSPEVIELTAEQRQGFREASRSVLKDHEKDIDMAIYEKIMKFQEAYPEE
                     APNS"
     gene            complement(3529..4215)
                     /locus_tag="P5219_01220"
     CDS             complement(3529..4215)
                     /locus_tag="P5219_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HRX25407.1"
                     /translation="MKPRILIINPNSSEEMTGAIHRNAAVFAGDDMEVLTVPTPGTSP
                     FIATYEDHAMAAPGMVRLLRENEDSFDAFVIACHGDPNIDLMKEITTKPVVGIAEASM
                     KMATMLGHSFSVIAPVERTVPNKKALIDRYGLARDMASVRPASVEQGADEAERLIAAG
                     RRAVEEDMAEVLVLGCAGFAGLDKRMEKELGVPVLDGVICALIVASGLVKYGVSISKK
                     RRYDNTFGKG"
     gene            complement(4212..>4593)
                     /locus_tag="P5219_01225"
     CDS             complement(4212..>4593)
                     /locus_tag="P5219_01225"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HRX25408.1"
                     /translation="ACIEMTLPLMLTAVRDGKLSMRRMVELLSENPARVFGLWPRKGA
                     IRVGGDADYVIADMKNEYTVNNKDFVTHSRDTSPMYNGFRLVGKPLHTIVRGRIIMKN
                     RVIDDSAEGWGKVMLPGWGSGRTA"
BASE COUNT          938 a         1325 c         1357 g          973 t
ORIGIN      
        1 gagaggggac gttcacgccg gagttgccgg gcatgttgta gatcatcacg gggatgggtg
       61 aggcgtcggc gagcatggtg tagaaattcc cgagggcggc ctcgttcatg tccttcttgt
      121 agtagttggg ggttacgacg agagccacgt cggcgcccac ggcggcgcat tccttcgtaa
      181 gctgcagagt ctccctgaag gactcgcatc ccgtaccggc gatgaccatt ttttccgccg
      241 gaagatggtt tctcgcggtc tccacgagct tcaccttttc ctcgtgggac aggagggtga
      301 actcgccgtt gctgccgagc accacgaggc cggacagttt cgtggcgccg aaggccaccg
      361 tgttctccgc aaaggtcgaa taatccacct ctccggaagc atcgaaggcg gtggcgatgg
      421 gggcgaaaat tccacggact tttttattca ttgcatttct ctctcctcgt aaaaaatata
      481 tgatattccg catatgatac ttcaaaacac tccaaaccgg aagggcgttt catccatgac
      541 gccttctacc tgaaaaactg cagcggccag agagacagca tgggaatgta ggtaatcatc
      601 agcaccgccg cgaggttcca gaccatgaac ggaatggagc ccctgagaac cttctcaaaa
      661 ggcgttttgc agatattcga cgccacgaag aggtccaccc ccatgggcgg cgtcgccatg
      721 ccgagggcga ggttcgccac cacgaaggtg ccgaaatgga tgggatccac gccgatcttc
      781 agcgccagcg gaaggaagat cgggcagaag atgaggatat tggacagggt atcgatgaac
      841 attcctccca ccaggaagac cgcgttgaac atgagcagga tgaggaactt gttggtggtg
      901 agggaaaata tgaggtttga aatgaggttc ggaacatcct tcatgtggag gagctttgca
      961 aaagcgctcg ccgtccccat gaggaaaatg catgaaccgg tgatgacggc cgcttccacc
     1021 atggcgctgc agagcccctt catcgtcagg ccgcccgtgg caaacccgag gacgatggcg
     1081 taggcgacgg ctacgattcc ggcttccgtc ggcgtgaaca caccggagta aatcccgccg
     1141 agaatgatga gaggcatgag aagagccgat ttcgcctgcc agaccctttt cagaacccag
     1201 cccgttcccc cttcccgggg cagccccgca tatccttttt tccttgaaat ataaacggcc
     1261 ggtatcatca gaaacagccc caccaggatt ccgggaaaga gcccggcgat gaagaggtcg
     1321 gtgatggaga ctccgctgtt cacgccgtag acgatcatgg gaacgctggg aggtatgagg
     1381 acgccgatgc cccccgcaca ggcaaccagc cctccggaaa atccgacccc gtagccccgg
     1441 gagatcatgg ccggcagaat gatggtgccg atggccgcca cggtggcagg ccccgaaccg
     1501 gagacggcgc cgaagaacat gcaggcaacg atggccgcta ttcccagccc gccgggaaag
     1561 tctccggcta ccgcttccgc aaggtctgtg agcttccgtg cgattccccc gtgggacatg
     1621 agggttccgg caagaataaa gaagggaagg gcgagaaggg tgaaggagtc ggctgtaacc
     1681 accatgcgct ggccgacgaa gaacatgacc cggtcgagtc ctccgggaat ccaggggatg
     1741 aaggcgaggc agctgaaccc tatggcagcg gctatgggga tactgagcag caacgcgaga
     1801 aacattcctc cgaatacggc ggtcagcatt ccggcatcct cctcatttga gagtctccgt
     1861 ttgttcctcc cgggggaaga acacgggcag gatgtccttc tgcacgatgc ggaaagccat
     1921 cagcaaaaaa aggacgggac ccagggagaa gtaggtccag gtgggcatat ccatggccgc
     1981 cgtggtctgg ccgctttcaa actggagccg ggttatgttg taggaatatt tcacaacaag
     2041 aagggtgaat ccgaggaacg ccagggacgc aaaaagcgat accgccttcc cgagcttcgg
     2101 gggcaggctc atggtgagga tttccacttt gacatgctgc cttttcttcg cgcagagaga
     2161 aaagctcacg aggctcaggg ccacgaagag aatccgcatc agctcttcgg cccacgaatg
     2221 gacaaaccgg agcagatagc gggacacaac ctgttccatc cctatgagca gaatggcgga
     2281 gaagaggatc accgcgaggg cttcctcaaa ataccggtcc gctaggctca gaattttcat
     2341 tgtcgggcac ctccgggaca gatcggggga gaggggagcc ctctcccccg gaataaacgg
     2401 actagctgtt tggggcctct tccggatagg cttcctggaa cttcatgatc ttttcgtaga
     2461 tggccatgtc gatatccttt tcatgatcct tgagcacgga cctgctggcc tcccggaatc
     2521 cctgccgctg ctcggcggtg agctcgatga cctcgggaga gggcgcggct ttgaactctt
     2581 ccatgatttt cttgttgttc gcgaggacat tgtcctgttc ccatgtgcag gtctcgtcaa
     2641 cagccttttg caggactgcc ttttcctcgt cggagagttt gtcccacgat tttttcgaca
     2701 tgacgatcag catggggtcc agaatgtgtc cgtcggtggt gatgtacttc tgcaccgtgt
     2761 ggaacccctg ggacttgatg ttttcgaagg tgttctcccc tgcgtcgatc acgccctgct
     2821 gcagggaggt gaacacttcg ccgaaatcca tgggaatcgg gttcgctccc atggccttga
     2881 atatggccat gcggatgggt gcgggttctg tgcggatttt gacgcccttc aggtcttcgg
     2941 gcttccggac ggcgatattg ctgtgggtat gccgccatcc catgatgaag tagccgaggg
     3001 gcttgatccc tttttcttca aggggcttca ccacttccat ggcaagatcg ctgttcacaa
     3061 aatcgagggc ggccttctcg ctcttgaaga gatagggcac gtcgtacact gtgacggctt
     3121 ttgtaaaggg agccaggagt cccgtcgaga tgagccagaa gtcggtgact ccctgctgga
     3181 tctgttcgat gccttccctg ttgtttccca gggcggccgc tcccatgacc tggaccttca
     3241 ttttcccgcc ggagtgcttg tccagcagct cggcaaattt ttcgcccgcc tgggcgaagg
     3301 tcaccagggg ctgtccggtg tggcccataa cgaattcctt cgcataggac accgctgaga
     3361 aggaaagaac cagcgcagcc agaagaacgc aaaaaaccaa ccgtttcatc gtagtcatct
     3421 ttcgtgtcgc ctcccttgtg atagtcgttc ccggttccgg tacctgcttc ctgtgcattc
     3481 cgttccgttt ctccgtactc tcacccccgt cctgtccggt cccctttttc agcccttgcc
     3541 gaaggtattg tcgtaccgcc tctttttgct gatggaaacg ccgtacttca ccagtcccga
     3601 agcgacaatg agggcgcaga tgacgccgtc gagcaccgga acgcccagtt ccttctccat
     3661 tctcttgtcg aggccggcga aaccggcaca gccgagaaca agcacttccg ccatgtcctc
     3721 ctccaccgct ctccgtcccg ccgcgatgag ccgctccgcc tcgtccgccc cctgttcgac
     3781 gcttgccggg cggacggagg ccatgtccct cgcaaggccg tacctgtcga tgagggcttt
     3841 cttgttggga accgtacgct ccacgggggc aatcacggag aagctgtgcc ccagcatggt
     3901 ggccattttc atggaggctt ccgcgatgcc caccaccggt ttcgtggtta tctccttcat
     3961 caggtcgatg ttcgggtcgc cgtggcaggc gatgacaaag gcatcaaaac tgtcttcgtt
     4021 ctccctcaga agccggacca tgccgggtgc ggccatggcg tgatcctcgt aggtggcgat
     4081 gaagggcgat gttcccggcg tgggaaccgt gaggacctcc atgtcgtccc cggcgaagac
     4141 ggcggcgttc ctgtggatcg ctccggtcat ctcctcgctg ctgttggggt tgatgatgag
     4201 aatccggggc ttcatgcggt cctccctgac ccccagccgg gcagcatcac ttttccccag
     4261 ccttcggcgg agtcgtcgat cacccggttc ttcatgatga tcctgccccg gacgatggtg
     4321 tgcaggggct tcccgacgag gcggaacccg ttgtacatgg gggacgtgtc cctggagtgg
     4381 gtcacaaaat ccttgttgtt caccgtgtat tcgtttttca tgtccgcgat gacgtagtcg
     4441 gcgtcaccgc ctacacggat ggctcccttc cggggccaga gaccgaagac ccgggccgga
     4501 ttctcggaga gaagctccac catgcgcctc atggaaagct tgccgtcccg cacggcggtc
     4561 agcatgaggg gaagggtcat ctcgatgcat gca
//
LOCUS       DAOTHD010000038         4547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_22607_length_4547_cov_3.238201, whole genome shotgun sequence.
ACCESSION   DAOTHD010000038 DAOTHD010000000
VERSION     DAOTHD010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22607_length_4547_cov_3.238201"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..1673
                     /locus_tag="P5219_01230"
     CDS             81..1673
                     /locus_tag="P5219_01230"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HRX25409.1"
                     /translation="MLDLVIKNALVVDGTGERKPFPADVGVQGGKIALVGTIDQEAAK
                     VTDAAGLALAPGFIDMHSHTDLEYFREKPSDAKIRQGITTEVLGQDGLGAAPVGGENL
                     QIVKDLLAGLDGIIPDEKWTWKTFPEYLGALERRGLNNNAAVLLCHGPVRIAALGMEE
                     RAATPAELRNMKALVRESMEEGAFGLSTGLIYPPCPYGDTEELIGLNREVGAKGGIFV
                     VHQRDEGYYLSRSFDEVSRISLESGAHLEVSHLQAYGRVNWPIMDEVMEKAERMVEKG
                     GSISWDRYPYLAGCTVLTAVLPNWTFNEGTAALVRNLRKPEFRARVHADFTKGLDVWH
                     NRQISVGWENIVVTAVQLEENKWMEGLSCRAIADRLGKDPIDMVMDLLAAEKLAVTMI
                     SFYGSDTVLEKVLGHPRGTVGSDGIFGGKPHPRLYGAYPCFFRRFVREKPFFTLEEAV
                     RKVTSFPASILGIPDRGRIEEGCWADMVIFDPDKIADRSTYDIPEAYPEGIPHVFVNG
                     TAVVEEGETVTCTPGKVLRHGK"
     gene            1699..1944
                     /locus_tag="P5219_01235"
     CDS             1699..1944
                     /locus_tag="P5219_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25410.1"
                     /translation="MDTGTLCRFFFWCTLINYLLLGGMTLGVILFGDKIYALHRRWFD
                     FDRGTFEAAMYFFLGIYKILVLVFFFVPWIALLIAGR"
     gene            complement(1928..2698)
                     /locus_tag="P5219_01240"
     CDS             complement(1928..2698)
                     /locus_tag="P5219_01240"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HRX25411.1"
                     /translation="MMNARSPQRKIWLVAAAAVLAASVAAAVVLFSEGEDAWRVFLKL
                     LQAVKRSGPFGPLVFLALFTAACIFLLPTFYLTIAAGLLFGLWEGFAVATVSVAAGAS
                     AAFLIARHRVRSGMLRLWGGETRFLALDEAVTEDGWKIVFLTRLSPAFPFNIVNYAFG
                     LTSVSFPVYLAASWTGSIPWTLMYVYSGSLAADMAGLHPEAVRDPNLHWTLSAFGLMA
                     TVAATCMAARTADRALKKRLRLEEKRAEAKEEDFSGLR"
     gene            complement(2793..3377)
                     /locus_tag="P5219_01245"
     CDS             complement(2793..3377)
                     /locus_tag="P5219_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HRX25412.1"
                     /translation="MKSLKGTKTEENLMKAFAGESQARNRYSYYSSAAKKEGYVQISN
                     IFAETAENEKEHAKLFFKFLVKDVNGSMVTIHADYPAAFGDTKENLLAAAEGEHEEWG
                     TLYPEFAKTAEEEGFREVARVFREVAEVEERHEKRYRKLHANLAAGTVFKKDTPVEWK
                     CNNCGYVHTGREAPELCPACAHPKAHFEVFVETY"
     gene            3567..3836
                     /locus_tag="P5219_01250"
     CDS             3567..3836
                     /locus_tag="P5219_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HRX25413.1"
                     /translation="MMKKKREITLCMGSSCFARGNARTLEVIRTFLKEHDIEAGVFLK
                     GSRCEGLCSKGPVVRVDDAVYTGVSPDGIEEILLREFGEGEDVHD"
     gene            3829..>4547
                     /locus_tag="P5219_01255"
     CDS             3829..>4547
                     /locus_tag="P5219_01255"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HRX25414.1"
                     /translation="MTDRVVSQDVVSFGSECQGCYRCIRECPVKAIRVERGQAAVIPE
                     LCVACGLCVEACPVQAIKPRLDLPRVKAMIASGRRVYASMAPTWACEFPGLDSSCLVA
                     ALKRLGFSGVGETALGAQAVCESLAKTLSDADPGLFLSTTCPSVVSYVEKYLPELAGT
                     LTKVPSPVEAHCRIFKEMVDPEGAVVFIGPCIARKDESDRSVDTIDAALTFRELRRWL
                     REEGVAPEKCVPGEEDRFFPEP"
BASE COUNT          863 a         1286 c         1472 g          926 t
ORIGIN      
        1 gccttcggag ggaagacgag caagaaggga ctgtacggcc cggacgcgta ggcggcggaa
       61 tttttgagga ggcggaaccg atgctcgatc ttgtgatcaa aaatgccctc gtggttgacg
      121 gcacggggga acggaagccc tttcccgcgg acgtgggagt gcagggcgga aaaatcgccc
      181 tggtgggaac aatcgaccag gaggccgcga aggtgaccga tgcggcggga ctcgcccttg
      241 cgcctggctt catcgacatg cacagccaca cggacctcga gtatttcagg gagaagccct
      301 cggacgcgaa aatccgccag ggcattacaa ccgaagtgct cggccaggac ggtctcggcg
      361 cggcccccgt gggcggggaa aacctgcaga tcgtgaagga cctcctggcg ggccttgacg
      421 gcatcatccc cgatgaaaaa tggacatgga agaccttccc ggaatatctc ggcgccctgg
      481 agcgcagggg gctgaacaac aacgccgccg tgctgctgtg ccacgggccg gtgcggatcg
      541 cggccctcgg catggaggag cgggcggcca ctcctgcgga gctccggaac atgaaggccc
      601 ttgtccggga gtccatggag gagggagcct tcggcctctc cacagggctg atctatccgc
      661 cctgccccta cggggatacg gaggagctta tcgggctgaa cagggaagtg ggagcgaagg
      721 ggggcatttt tgtcgttcac cagcgggacg agggatatta cctctcccgt tccttcgacg
      781 aggtttccag gatttccctg gagtcgggcg cccatctcga ggtctcccac ctgcaggcct
      841 acggcagggt gaactggccg atcatggacg aggtcatgga aaaggcggaa cggatggtgg
      901 aaaagggggg cagcatttcg tgggacaggt atccctacct tgcgggatgc accgtcctta
      961 cggcggtcct gccgaactgg accttcaacg aggggaccgc cgccctggtc cggaatctcc
     1021 ggaagccgga gttcagggcc cgggtgcacg cagacttcac gaaaggtctt gacgtgtggc
     1081 acaaccgcca gatctccgtg ggctgggaga acatcgtcgt gacggccgtc cagctcgagg
     1141 agaacaagtg gatggaaggc ctgagctgcc gggcaatagc ggaccggctg gggaaggacc
     1201 ccatcgacat ggtcatggat ctgctcgccg ctgaaaagct cgcggtgacc atgatcagct
     1261 tttacgggtc cgacacggtg cttgagaagg tgctcggcca tccccggggg accgtgggaa
     1321 gcgacggcat cttcggcggg aagccccatc ccaggctcta cggggcgtac ccctgcttct
     1381 tccggcggtt cgtgcgggag aagccgttct tcacgctgga ggaggcggtc cggaaagtca
     1441 cgtccttccc ggcatcgatt ctcggcatcc ctgaccgggg gaggatcgag gagggctgct
     1501 gggccgacat ggtgatcttc gatcctgaca aaatagcgga caggtccact tacgacattc
     1561 cagaggccta cccggagggc attccccatg ttttcgtcaa cgggacggct gtggtggaag
     1621 agggggaaac ggtcacctgt accccgggga aggtcctgag gcacgggaag tgacggggaa
     1681 aacgggggag gtgtttttat ggatacggga acgctgtgcc ggtttttctt ctggtgcacc
     1741 ctgatcaact acctgctcct gggggggatg acccttgggg ttatcctttt cggcgataaa
     1801 atttacgctc ttcaccgaag gtggtttgat ttcgacaggg gaacctttga agcggccatg
     1861 tatttctttc tgggcatcta caagatactg gtcctggtct ttttctttgt tccctggata
     1921 gccctgctta tcgcaggccg ctgaaatctt cctcttttgc ctccgccctc ttttcctcga
     1981 ggcggaggcg ttttttcagc gccctgtcgg cggtgcgggc cgccatacag gtggccgcga
     2041 cggtggccat caagccgaag gccgacaggg tccaatggag attcggatcc cggacagcct
     2101 ccggatggag accggccatg tccgcagcca gggatcccga gtagacatac atgagcgtcc
     2161 aggggatgct cccggtccat gaagccgcaa ggtagaccgg aaaggacacc gacgtaaggc
     2221 cgaaggcgta gttcacgatg ttgaaaggaa aagcggggga gagccgggta aggaagacga
     2281 tcttccatcc gtcctccgtg acggcttcat ccagggcgag gaaccttgtt tcccctcccc
     2341 acagcctgag catgccggac ctgacgcggt gccgggcaat gagaaacgcc gcggatgctc
     2401 ccgccgccac cgacacggtg gcgacggcaa aaccctccca caggccgaag aggagtcctg
     2461 cggcgatggt gaggtagaac gtgggcagca gaaagatgca cgcggcggta aaaagggcga
     2521 gaaatacaag gggtccgaaa gggccggaac gcttcaccgc ctgaaggagt ttcaggaaaa
     2581 ccctccaggc gtcttcccct tccgaaaaaa gaacaacagc cgccgccacg gatgccgcca
     2641 ggactgccgc tgcggcgacc agccatattt ttctctgcgg gcttcgcgcg ttcatcatgc
     2701 accacccttt caaaagggcg gcttcatccc cggatcatgt tcgtcttccc ggaagggaac
     2761 ccggacgaaa tccggggatg aatgtctgct cttcagtatg tttcgacgaa cacctcgaag
     2821 tgggccttcg gatgggcgca ggcggggcag agttccggcg cttccctgcc ggtgtggaca
     2881 tagccgcagt tgttgcactt ccattccacg ggggtgtcct ttttgaacac cgtaccggcg
     2941 gccaggttgg catggagctt gcggtaccgt ttctcgtgcc gttcttccac ctcggccact
     3001 tcgcggaaca cccgggccac ttccctgaag ccttcttctt cggcggtttt cgcaaactcg
     3061 ggatacaggg ttccccactc ctcgtgctct ccctcggctg cggcgaggag gttttccttc
     3121 gtgtcgccga aggccgccgg ataatcggcg tgtatggtca ccatgctgcc gttgacatcc
     3181 ttcacaagga acttgaagaa aagcttcgcg tgctccttct cattttcggc cgtctcggcg
     3241 aagatgttgg aaatctggac gtacccttcc ttttttgccg cggatgagta gtagctgtac
     3301 ctgtttcgcg cctgcgactc gcccgcgaag gctttcatga ggttttcttc tgttttcgtt
     3361 cccttgagtg atttcaatgc gattcctccc ggtgtgatag tgatgacaag ttcagcaaat
     3421 attataccat tccttttcag agcgttgtcg gtcattctca tgtggtgatt tattggtatt
     3481 tttctatatt tgtattctga tttagtgaaa agtgtaactt tatagtatca ttgccgtgaa
     3541 gtacgatcaa ggaggcgctg tgcgtcatga tgaaaaaaaa gagggagatc accctctgca
     3601 tgggcagctc ctgttttgcc agggggaatg cccgtaccct ggaggtgatc aggacctttc
     3661 tgaaggagca tgatatcgag gccggggttt ttctcaaggg aagccggtgc gaggggctgt
     3721 gttcgaaggg gcccgtggtg cgggtggatg atgccgttta tacgggagtg tcccccgatg
     3781 gcatagagga gattctgctc agggaattcg gggaagggga ggatgttcat gactgaccgc
     3841 gtggtctccc aggacgtggt gtcctttggt tcggaatgcc agggatgcta ccggtgcatc
     3901 cgggaatgcc cggtgaaggc gatccgcgtc gagcgggggc aggcggcggt cattccggag
     3961 ctgtgcgtgg cctgcggcct gtgcgtggaa gcctgccccg tccaggcgat aaaaccccgc
     4021 ctggatctcc cccgggtgaa ggctatgatc gcctccggga ggagagtgta cgcctccatg
     4081 gcccccacgt gggcctgcga gttccccggc ctggattcct cctgcctggt ggccgccctg
     4141 aagcgcctgg gcttttccgg cgtgggggag acggccctcg gcgcccaggc ggtgtgcgaa
     4201 tccctggcga agaccctttc cgacgccgat cccggactct tcctctccac cacctgcccg
     4261 agcgtggttt cctacgtgga aaaatacctc cccgaacttg ccgggaccct cacgaaggtt
     4321 ccctctcccg tggaggccca ttgccggatc ttcaaggaga tggtggatcc ggagggcgcc
     4381 gtggtgttca tcgggccgtg catcgcccgg aaggacgagt ccgaccggtc ggtggacacc
     4441 atcgacgcgg cactcacctt ccgggaactc cgccggtggc tccgggagga aggagttgcc
     4501 ccggagaagt gcgttccggg ggaggaggac cggtttttcc ccgaacc
//
LOCUS       DAOTHD010000039         4532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_22700_length_4532_cov_2.729730, whole genome shotgun sequence.
ACCESSION   DAOTHD010000039 DAOTHD010000000
VERSION     DAOTHD010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22700_length_4532_cov_2.729730"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="P5219_01260"
     CDS             <1..826
                     /locus_tag="P5219_01260"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HRX25415.1"
                     /translation="KKAALGLAIATLLTAVFLGPLSSLFFLLGFGVLGIGLGFLARRC
                     SSGVEVMLLGILLSLGSKLLLMVIAAKVTGINPFQLDGAEMQAMVDRIFGFYETAGMS
                     RESLSAVKQQFSQAIGVLPVIFPTILTMAAALDCYLSYRVSGFVLKRIGGTSLPPLPP
                     FSRWRFPKSVFGALLASVLLSVFGSQGGEWNMAMRIGTNVRMLVNFLFLFQGLSLFWY
                     FLGGGDSPHRGAGRFLRTVAIIVAVLVPMLSTATVMVGIADMWLDFRTRFRRNDER"
     gene            823..1269
                     /gene="rplI"
                     /locus_tag="P5219_01265"
     CDS             823..1269
                     /gene="rplI"
                     /locus_tag="P5219_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRX25416.1"
                     /translation="MKVILKQDVNKIGAAGELIETSDGYARNFLFPKGLAEEATPERL
                     KEWKDRQAAKKKKDEKLKNEALELQKRLSGKVVRVRASTGEKGKLFGSVTAAAVAENL
                     AAQHSASVDKRDIRMPDTVKQTGNYTFTVRLYPGIEAEMTLTVESE"
     gene            1266..2621
                     /gene="dnaB"
                     /locus_tag="P5219_01270"
     CDS             1266..2621
                     /gene="dnaB"
                     /locus_tag="P5219_01270"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRX25417.1"
                     /translation="MMAAHPSWDRTPPVSPEAERAALGACLMDREALNIVLEILQPED
                     FYDLNHRVAYEIVYEMAQRDKPVDPLTFLEELSRQGKSERLGGQPFVASLVDSVPTTA
                     NVEYHAGIVRDKAIHRRLISAGNTIVKLGYSEELDVDEVLEEAERAVFEIAQKRNTTN
                     FRHIGEVLGKTFLQIEEQYNRSDQDVAGFNSGFYELDRLMGGFQPGSLNIVAARPSMG
                     KTAFAINIAQFGGQGMRRPVLIFSLEMGAEQLVQRMLGAEARINIHDLRNGSFPKSSW
                     ETLAEAAGRLAEAPVYIDDSSMLSTLELRARCRRFKSRHKDLGLVVVDYLQLMNSSRR
                     IENKQQEVAEISRGLKGVARELEVPVIALSQLSRAVEQRTEKKPQLSDLRDSGAIEQD
                     ADTVMLLYRPGYYEAGAPGEEDDNRAFINLAKHRNGPTGEISLVFLREYTRFVNADRS
                     Y"
     gene            2972..3775
                     /locus_tag="P5219_01275"
                     /pseudo
     CDS             2972..3775
                     /locus_tag="P5219_01275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
     gene            3782..>4532
                     /locus_tag="P5219_01280"
     CDS             3782..>4532
                     /locus_tag="P5219_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRX25418.1"
                     /translation="MNEALKGGRTLPAQEEPGSLILPSCLFPLALFVSLVLPNLVFSG
                     PWFYSTLHLMKWGVALVPVALCGLLAGITVTVRGTVRTAFTIDGFAVVWLLLLLYVTA
                     QPFWSGLRSPETFFQEWFFFASLWLVYVLSSRLTGGRLLRILLWGSLVNCAVSVVFAE
                     LQVLEIRDLPFFILPTPGHYIANTGQQNMFALWLALGSLNGAFLFFSAGKNGRIVRAI
                     LVPLLAVTFWGLISTTSRSGILALLTGFAVLS"
BASE COUNT          897 a         1413 c         1349 g          873 t
ORIGIN      
        1 gaaaaaagcc gctctcggcc tggccatagc caccctgctg acagccgtat tcctcggccc
       61 cctgtcatcc cttttcttcc ttctcgggtt cggcgtcctg gggatcggtc tcggcttcct
      121 ggccaggcgg tgctcttccg gcgtggaggt gatgctcctc ggcatcctcc tctccctggg
      181 aagcaagctc cttcttatgg ttatcgccgc aaaggtgacg ggaatcaatc ctttccagct
      241 cgatggggcc gagatgcagg ccatggttga caggatcttc ggcttctacg aaacggcggg
      301 aatgtccagg gaatccctgt cagcggtgaa gcagcagttt tcccaggcca taggggtgct
      361 cccggtgatc ttccccacca ttctcaccat ggcggcggcc ctggactgct atctcagcta
      421 cagggtgagc ggcttcgtcc tgaagcggat cggcggaacc tcccttcctc cgctgcctcc
      481 cttttcccgc tggagattcc cgaagagcgt cttcggtgcg cttctcgcct cggtgcttct
      541 ctcggttttc ggatcccagg gaggggaatg gaacatggcc atgcgcatcg gcaccaatgt
      601 ccggatgctc gtgaacttcc tgttcctctt ccagggactc tccctctttt ggtatttcct
      661 cgggggaggg gacagtcccc acaggggggc gggacgtttc ctcaggaccg ttgctataat
      721 agtggcggtt ctcgtgccca tgctttccac ggccaccgtc atggtgggca tagcggatat
      781 gtggcttgat ttcagaaccc gtttcaggag gaatgatgaa cgatgaaagt aatattgaag
      841 caggacgtga acaagatcgg cgccgcagga gaactgatcg agacttctga cggctacgcc
      901 cgaaatttcc tcttcccgaa gggacttgcc gaggaggcca cgccggagcg cctgaaagag
      961 tggaaagacc ggcaggcagc gaagaagaaa aaggacgaga agctgaaaaa cgaggccctt
     1021 gagcttcaga agcgcctttc cggaaaagtt gtccgggtcc gggcgagcac cggcgagaag
     1081 ggcaagctct tcggaagcgt caccgccgcc gcggtggcgg agaacctggc tgcccagcat
     1141 tccgcgtccg tggacaagcg ggatatccgg atgcccgaca cggtaaagca gacagggaac
     1201 tatacattca ccgtccgcct ctatcccggc atcgaggctg agatgaccct taccgtcgag
     1261 agcgaatgat ggccgcccac ccgtcatggg accgaacgcc ccccgtctcc ccggaggcgg
     1321 agcgcgcagc tctcggcgca tgtctcatgg accgggaggc gctcaatatc gtccttgaga
     1381 ttctccagcc ggaggatttt tatgacctga accacagggt cgcctacgaa atcgtctacg
     1441 aaatggccca gagggacaag ccggtggatc ccctcacctt cctggaggaa ctctccaggc
     1501 agggaaaaag tgaacgcctg ggggggcagc cctttgtcgc ttccctcgtg gacagcgtcc
     1561 ccacaacggc caacgtggaa taccatgcgg gcatcgtccg ggacaaggca atccaccggc
     1621 ggctcatctc cgcggggaac accatcgtca agctcggtta ttccgaggag ctggacgtgg
     1681 acgaggttct cgaggaagcg gaacgggcag tgttcgagat cgcccagaag aggaacacca
     1741 ccaacttccg gcacatcggc gaagtcctcg gcaaaacctt cctccagatc gaggagcagt
     1801 acaaccgttc ggaccaggac gtggcgggtt tcaattcggg attctacgaa ctcgacaggc
     1861 tcatgggcgg attccagccg ggaagcctga acatcgtggc cgcccgtccc tccatgggaa
     1921 aaacggcctt tgccatcaac atcgcccagt tcggcgggca gggaatgagg cgcccggtgc
     1981 tcatcttcag cctggaaatg ggcgcggagc agcttgtcca gcggatgctg ggcgccgagg
     2041 ccaggataaa catccacgac ctccggaacg gctcctttcc gaaatcctcc tgggaaactc
     2101 tcgctgaagc tgccggccgg ctcgccgagg ccccggtcta cattgacgac agctccatgc
     2161 tgtccaccct ggaactccgg gcacggtgcc gccgcttcaa gtccaggcac aaggacctgg
     2221 gactggtggt ggtggactac ctccagctca tgaactcctc gaggcggatc gagaacaagc
     2281 agcaggaagt ggcggaaatt tcccggggac tgaagggagt ggccagggaa ctcgaagttc
     2341 ccgtcatcgc cctgtcccag ctctcccggg cggtggagca gaggacggag aagaagccgc
     2401 agctttccga cctgcgggac agcggcgcta tcgagcagga cgccgacacg gtcatgctgc
     2461 tctaccggcc cggctattac gaggcaggtg ccccggggga ggaagacgac aacagggcct
     2521 tcatcaacct ggccaagcac aggaacggcc ccaccgggga aatctctctc gtcttcctca
     2581 gggaatacac ccggttcgta aatgccgacc gttcctattg atcctaaatc cgcagttttt
     2641 ttcgggcaga gggagcgtcg ccgggaaaag cgcccgggcc gggaaaacgg catggatgtc
     2701 ggtttaaggt gcagttgtca tggacgacaa tctgcaccga cggcccaagc gagcaccggt
     2761 gacgctcccc gctgcctccg ggttttggtt gatttccctt gattggtcgg cgcacggtgg
     2821 agtagaatgc atgtgaagcc tttttcttcg cccaagcttt ggaaaccgga ggaacgacat
     2881 ggcgggagtc ctgaattttc tgaggagtga cacgggaaag taaaaaaatg ccggaaagaa
     2941 ccttccggag gacggacagg ccaggcggct caccgaaata ccggtcgacc tgatcagtcc
     3001 caacccgacc cagccccgcc gccatttcga cgaaggggac ctggacgatc tcgcggcctc
     3061 catttcccag gtgggcatca tccagcccgt agtggttctc gccctggaat cgggctacga
     3121 actcgtggtg ggcgaacgga ggctccgggc ggcaaaaaaa gccggccttt ccaccattcc
     3181 tgccatcatt accgaggcgg acccgggcga acagcaaatc ctggcccttg tggaaaacat
     3241 ccacaggagc aacctctcgg cggtggagga agccgccagc ctccaggcca tcatggagcg
     3301 ttccggctgg acccagacgg aacttgccgg aaagctgggc cgctcccagt cctccgtggc
     3361 aaacaagctc cggctccttc ggctcgagga accggtgcag cgtctcgtca tggagggaaa
     3421 gctcggcgaa cggcaggccc gggcactcct ctccctcccc ccggaagacc agatcgccct
     3481 cgccgcggaa gccgcagcgg agaacatggc cgccagcgaa gtggagcaga aggtaaagga
     3541 ccgcgccccg tcgcagtcaa agaccgggaa gaaacggctg aaaaagaatc ctctttcctt
     3601 ttccgggccc gacggcccca ccggggaaat cctccgtgac gtggccgccc tggtggaaca
     3661 gaacaggaaa aagggaatac ccgtggtctg gaaagtgaag gaactcgccc agagggagct
     3721 tgtggtggag ctcgtggtgg aactcaacgg ggatctcgga cagacggagg aatgacgcag
     3781 aatgaatgaa gcgctgaaag gcggacgcac ccttcccgct caggaggaac cgggctcgct
     3841 catcctgccc tcctgtttat tcccccttgc gctctttgtc tccctcgtgc tgccgaacct
     3901 cgttttttcc ggcccctggt tctattccac ccttcacctc atgaagtggg gggtcgccct
     3961 ggttcccgtt gcactctgcg gtcttctggc cggaatcacc gtaaccgtcc gcggcacggt
     4021 ccggacagcc ttcaccatag acggcttcgc cgtggtctgg ctgctgctgc tcctctacgt
     4081 gacggcccag cctttctggt cgggactccg gtccccggaa acctttttcc aggaatggtt
     4141 tttcttcgct tccctctggc tcgtctacgt tctttcctcc cgcctcacag gaggaaggct
     4201 gctgaggatc cttctctggg gcagcctggt caactgcgcc gtgagcgtcg tcttcgccga
     4261 gctccaggta ctggagatcc gggatcttcc gttctttatc ctccccaccc ccgggcatta
     4321 catcgccaat acggggcagc agaacatgtt cgccctctgg ctcgctctcg gcagcctgaa
     4381 cggcgccttt ctgttctttt ccgccggaaa aaacggaaga attgtccgtg ccatcctcgt
     4441 tccgcttctt gcggtcacct tctggggcct gatttccacc accagccgct cgggtatcct
     4501 cgcccttctc acggggttcg ccgtcctctc ct
//
LOCUS       DAOTHD010000040         4483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_23008_length_4483_cov_2.777326, whole genome shotgun sequence.
ACCESSION   DAOTHD010000040 DAOTHD010000000
VERSION     DAOTHD010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23008_length_4483_cov_2.777326"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="P5219_01285"
     CDS             complement(<1..246)
                     /locus_tag="P5219_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25419.1"
                     /translation="MKKTFRQAGAVLLLCIAWACTACSAGADEVVPLQTPEAALEVTE
                     KIMDKVSAGDLESALRMMRPYFRIPDAEFNILLEQSKM"
     gene            complement(483..1307)
                     /locus_tag="P5219_01290"
     CDS             complement(483..1307)
                     /locus_tag="P5219_01290"
                     /inference="COORDINATES: protein motif:HMM:NF009828.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HRX25420.1"
                     /translation="MKKLRVAILGCGTIGRFVLDHLADGVVPGCEPVAVYLRSPESRG
                     REHLESRKIPWVTSLEDLLAFSPDVVVEASTHDAVVAMGPEILTRGVDFIPLSLGAFV
                     DPGLMEIMTAAAEKGGSRLHIPSGGIGALDALQTALIGGVEKVTMTTRKYSTTWKGIP
                     AVDELGVDLENLKEPLLLFEGPARECMKKYPQSINIGAALSIAGIGFDRTIIKIYADP
                     TVEYNTHEIRWEGATGKVTVLFENTPVPTNPKTTYQACLSTLFVLKNMTGNRIIGA"
     gene            complement(1323..2294)
                     /locus_tag="P5219_01295"
     CDS             complement(1323..2294)
                     /locus_tag="P5219_01295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRX25421.1"
                     /translation="MKGKAWKGIALAAALMMCFGSPLTASALELKQVSIAGAGTAGTF
                     YIMAAGIADLFGKQLGVNSVAEVTAGSVENVKLLDSKRVELGVMQVDVMQNALADKAQ
                     FKAPVDMVAIAPLYPNVIQIVTLKDSPINTFADLKGKRISVGSPGSGILATNQIILET
                     LGMTLNDIQPQYLSFAETTNAFRDGSIDAAIVNTAAPAPWVVDLETSHEVKLIELSKE
                     EIEKFTGKFGHFVAAEIPKEAYKSLEKDVPTFAVWITLSGRGDLPEQDVYDMTKTIFD
                     NTDFLKGIHAVGSYITLENVSKIASLPFHPGAVKFYAEKGIDVSPKK"
     gene            complement(2362..4167)
                     /locus_tag="P5219_01300"
     CDS             complement(2362..4167)
                     /locus_tag="P5219_01300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HRX25422.1"
                     /translation="MITAVSAAMGIFHVYTAFFGVLTALWQRSIHLTFGFLICYLTVV
                     GGASLKREKIFPLIAAGASLFCLGHFIVNFQAMILRFGEPDALDLAVGTMLIVLVLDF
                     ARRSVGNILPGIAVVFLLYAVAGPYLPGMLWHRGYDVTRIIYQISLSTEGIFGTPLGV
                     SASYVFIFVLFGAFLEITGAGQFYIDFAVKAVGGSAGGPAKAAVVASSLFGSVSGSAV
                     ANVAGTGAVTIPLMRSIGYRPEFAAAVEAVASTGGQIMPPLMGAAAFIMAEILAVPYY
                     KVALGALIPALIFYGTVFTMVHCRAKVLGLSGAARDSLPPMKTLLREQGIFFIPLLVL
                     IYFMIIAQYSVMKAAVYSLLATVLVGFFKKRMGWRDILGAFESGARVSLVVIASTACA
                     GIIVAVMNLTGLGMKFSSLVLAAGGNSLLLVLIMIMLASLLLGMGLPTTPAYLILAVL
                     GAPTLVKLGVEPLAAHLFVFYFGCMSMITPPVGLAVYAASCIAEADFWKTSKHALLLA
                     LPAFLVPYMFVFHPSLVGLGDMVSVLSAAASGLLGAVILGAGLSGWYFGKVSLPERGV
                     LCAAGILLILPGWLSNAAGILPALAVWVFRKKLKI"
     gene            complement(4293..>4483)
                     /locus_tag="P5219_01305"
     CDS             complement(4293..>4483)
                     /locus_tag="P5219_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRX25423.1"
                     /translation="TTITFPQGRKKALEGHKRVLQALRRRSPDDAALQMARHIDEIEK
                     AQRFIMALQDGPVKDGAG"
BASE COUNT          910 a         1394 c         1322 g          857 t
ORIGIN      
        1 catcttgctc tgctccagca ggatattgaa ctccgcgtcg ggtatgcgga agtagggcct
       61 catcatccgc agggcgctct cgagatcccc tgccgagacc ttgtccatga tcttttccgt
      121 cacctccagc gccgcttccg gcgtctgcag gggaacgacc tcgtccgccc cggcggagca
      181 cgccgtgcat gcccaggcga tgcacaacag gagaacggct cccgcctgcc tgaatgtctt
      241 tttcatacta tcccctccgg aaatcaagat aggtgcacat tcgggaacgg aaaagggctc
      301 tttcccaccc tgttccgtac tatatagcat aaccccggct gccgcacccc cgttttttcc
      361 ggaaccgccc ggttctttca ggtaccggtt cccggagctg atgccccggc acgagagagc
      421 ggcccggacg aaaacgcccg ggccgctctc ttttcggctc tctctcggtt ctccggcggg
      481 ggtcaggcgc cgatgatcct gttccccgtc atgttcttca gcacgaagag agtgctgaga
      541 caggcctggt aggtcgtctt cggattcgtg ggaaccggcg tgttctcgaa gaggaccgtc
      601 acttttcccg tggcgccttc ccagcgtatc tcgtgggtgt tgtactccac cgtggggtcg
      661 gcgtagattt tgatgatcgt ccggtcgaag cctattcccg cgatgctcag ggccgcacct
      721 atgttgatgc tctgggggta tttcttcatg cactccctcg ccgggccctc gaaaaggagc
      781 aggggctcct tcaggttctc caggtcgacg ccaagctcgt ccacggcggg aatgcccttc
      841 caggtggtgg agtatttcct ggtggtcatg gtcaccttct ccacgccgcc gataagggcg
      901 gtctgcaggg cgtccagggc gccaatgccc cccgagggga tgtgaagcct gctccctccc
      961 ttttcggccg ccgccgtcat gatctccata agaccgggat ccacgaaggc cccgaggctc
     1021 agggggatga aatccacacc ccttgtcagt atttcaggcc ccatggccac cacggcgtcg
     1081 tgggtggacg cctccaccac cacgtccggc gaaaacgcga ggaggtcttc gagactggtg
     1141 acccagggga tttttctcga ctcgagatgc tccctccccc tggattccgg cgaacggaga
     1201 tagaccgcca ccggctcgca cccgggcacg accccgtcgg cgaggtgatc aaggacaaac
     1261 cgcccgatgg taccgcagcc aaggatggcc acgcgcagtt ttttcaacgg tactcctcct
     1321 ctctatttct tcggcgaaac gtcgatcccc ttttcggcat aaaacttgac ggcaccggga
     1381 tggaagggaa gggaggcgat cttcgacacg ttctccaggg tgatgtagct tcccaccgcg
     1441 tggatgccct tcaggaaatc ggtgttatcg aaaatggtct tcgtcatgtc gtagacatcc
     1501 tgctccggaa ggtcgccccg gcctgacagg gtgatccaca cggcaaaggt ggggacatcc
     1561 ttttcaaggg acttgtacgc ctctttgggg atttccgccg cgacgaaatg gccgaacttt
     1621 cctgtgaact tttcaatctc ttccttcgaa agctcgatga gcttcacttc gtggctcgtc
     1681 tccaggtcca ccacccaggg ggcaggggcg gcagtgttca cgatggccgc gtcgatggag
     1741 ccgtcacgga aggcgttggt ggtctcggcg aaggagaggt actggggctg gatgtcgttc
     1801 agcgtcattc ccagagtctc caggatgatc tggttcgtgg cgagaatgcc gcttccggga
     1861 gagcccacgg agatcctctt gcccttcagg tcagcgaagg tgttgatggg ggaatccttc
     1921 agcgtgacga tctggatcac gttggggtac aggggagcga tggccaccat gtccaccggg
     1981 gccttgaact gggccttgtc cgccagcgcg ttctgcatga cgtcaacctg catgacgccg
     2041 agttccaccc gcttgctgtc caggagcttg acgttctcca cagagcccgc cgtcacctcg
     2101 gccacggagt tcacgccgag ctgtttgccg aacaggtcgg cgatacctgc ggccatgatg
     2161 taaaacgttc ccgccgtacc ggctcccgcg atggaaacct gcttcagctc aagggccgaa
     2221 gccgtcagag gtgacccgaa acacatcatg agcgctgccg caagggcgat tcctttccat
     2281 gcctttcctt tcatcttctc ttcctccttt gaatggtgtg ctttcttttc actgctctcc
     2341 gaaaggacgc tgctccgagc gtcagatctt cagcttcttc cggaataccc ataccgcaag
     2401 cgccggcagg attcccgccg cgttgctcag ccagcccggc agaataagga gaatgcctgc
     2461 ggcgcagagg acaccccgct ccggaaggga gaccttcccg aaataccagc cggaaagacc
     2521 cgccccgagg ataacggccc cgagcagtcc gctcgccgcc gcggagagga cggacaccat
     2581 atctccaagc cccaccagcg acgggtggaa tacgaacatg tacggcacca ggaatgccgg
     2641 aagggccagg agaagggcat gcttcgacgt tttccagaaa tccgcctcgg cgatgcagga
     2701 cgccgcatag acggccagtc ccaccggcgg ggtaatcatg gacatgcagc cgaaatagaa
     2761 cacgaaaaga tgggctgcca ggggttccac tcccagcttc accagggtgg gggctccgag
     2821 gaccgccagg atgagatacg ccggtgtggt ggggagtccc atgcccaaca gcaggctggc
     2881 gagcatgatc atgatgagga caagaagaag gctgttccct ccggcggcaa ggacgagact
     2941 cgaaaacttc atgcccaggc ccgtgagatt cattacggcc acgatgatcc cggcacaggc
     3001 agtcgaggcg atgaccacca gggaaaccct cgcgccgctt tcaaatgcgc cgaggatgtc
     3061 ccgccagccc atccgcttct tgaaaaagcc caccaggacc gtggcaagaa gagaatagac
     3121 ggccgccttc atgacggagt actgggcgat gatcatgaag taaatcagca cgagaagggg
     3181 aatgaaaaaa atcccctgct cccgcagcag ggtcttcatg ggagggagag agtccctggc
     3241 cgcgccggag aggccgagca ccttcgccct gcagtgcacc atggtgaaga ccgttccgta
     3301 gaaaatcagg gcgggaataa gggctcccag ggccaccttg tagtagggaa cggccaaaat
     3361 ctccgccatg atgaaggctg ccgctcccat gaggggaggc ataatctgcc ctcccgtgga
     3421 ggccaccgcc tcgaccgcgg cggcgaactc cggcctgtag ccgatggagc gcatgagggg
     3481 gatggtcacc gccccggtac ccgcgacgtt tgccacggcg ctgcccgaaa cggatccgaa
     3541 gaggctgctc gctaccacgg ctgcctttgc cgggcctccg gcgcttcctc cgactgcctt
     3601 cacggcgaaa tcgatgtaga actgccccgc cccggtaatt tcaagaaagg ccccgaagag
     3661 gacgaagata aagacgtagc tcgccgacac cccgaggggc gtaccgaaaa tgccctcggt
     3721 gctcagggag atctggtaga tgatccgggt cacgtcgtac cccctgtgcc agagcatccc
     3781 gggaagatag gggccggcga cggcgtagag aagaaacacc acggcgatcc cgggaaggat
     3841 gttccccacc gaccggcgtg cgaagtcgag aacgagcacg atgagcatgg tccccaccgc
     3901 gaggtccagg gcatcaggct ctccgaaacg gagaatcatg gcctggaagt tgacgatgaa
     3961 atggcccagg cagaaaaggg aggccccggc ggcaatgagc gggaatatct tttcccgctt
     4021 cagggacgct cctcccacaa cggtcagata acagatgaga aatccgaagg taagatggat
     4081 gctccgctgc cacagtgcgg tcagcacccc gaaaaacgcg gtgtagacgt ggaaaatgcc
     4141 catggccgct gacacggccg ttatcacgag attcgttttt ctctcaggaa aacattccaa
     4201 aatatacatc cccccgcaca gcagcttttc ctgattcccc tacgcacaaa aaaccatatt
     4261 ccgtccggcc tcttccccct ttcaagcccg gattatccgg ctccgtcctt caccggaccg
     4321 tcctggaggg ccatgatgaa ccgctgggcc ttctctattt cgtcgatatg ccgggccatc
     4381 tgaagggctg cgtcgtcggg cgaacgcctc cgcagggcct ggagcactct tttgtgtccc
     4441 tcgagggctt ttttccgccc ctggggaaag gtgatggtgg ttg
//
LOCUS       DAOTHD010000041         4470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_23091_length_4470_cov_3.254360, whole genome shotgun sequence.
ACCESSION   DAOTHD010000041 DAOTHD010000000
VERSION     DAOTHD010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23091_length_4470_cov_3.254360"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="P5219_01310"
     CDS             <1..894
                     /locus_tag="P5219_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRX25424.1"
                     /translation="NAATRTAALLTGEVDIIEDVPVRDVDKLKNTEGLSVVDRPSERL
                     IYLHVDSHRPQGPGIIGLDKNPLADLRVRKALSLAINRDSIVKMIMNGNAYATNQMVL
                     KGRRGYVEDMPAPKYDPEEAKKLLAEAGYPEGFKVYLDAPNGRYPNDGQVAQALASQL
                     TKAGILIELRLHPKTTFFDFVRPGDKSSLVMTGWSEPIDVGEMASVLFYTRGKNPAKG
                     GSNRGHYANPGFDALVDEADGTADPGKRRVILEKAARMIVEDGGIVPLYFQQDLYGKK
                     DAVVFEPRADKSILAYDMDVK"
     gene            984..2156
                     /locus_tag="P5219_01315"
     CDS             984..2156
                     /locus_tag="P5219_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRX25425.1"
                     /translation="MKDRIINRVEEIGKELLQLSRDIHGCPETAFEERKAARWQKELL
                     ERHGFTVEMPFCAMETAFRAVKKGREEGPTVAFLSEYDALKGLGHACGHNIIAASSVG
                     AGIALGSVLEDIAGEVQVIGTPGEEEGGGKIYMVERGCFAGVDFALMVHPNTKNLIGR
                     GGLAAQSVRIEFLGRAAHSAVPDKGVNALTSLIAFFNGIDTLRQTWPNSAKINGIITR
                     GGTASNVIPDLAEACFTVRASTKKELNAMYRDLNRVAEAAAMVTGASFLLEGEPVYAE
                     RYPNSAMGEAFKRNMESLGETMEYPDPRERFGSSDVGNVSLELPTIHEYLAIADTSVA
                     GHTVEFREAAVSPRGGEVVLLAAEGLALTGWDLLTDGDLREAAREEYRRKVLPFRD"
     gene            2434..3258
                     /gene="proC"
                     /locus_tag="P5219_01320"
     CDS             2434..3258
                     /gene="proC"
                     /locus_tag="P5219_01320"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRX25426.1"
                     /translation="MENTVRIAILGAGNIGTSIARGLAQAGNFTPGMITLTRRRTHLL
                     EEYKAMGFEVTSDNPAAVKGADVVIVAVEPQRVDTLLQEIAPALDFDRHILISVVTGL
                     AIKRIEEVIGKPLRIARAMPNTAIAVRESMTCLASNGPDDRALEMAKRIFNDVGKTLR
                     IREEDMIAATALGACGIAFFLRAIRAASQGGIEIGLSAKNSLAMAVQTAKGAACLLSV
                     SGSHPENEIDKVTTPKGCTISGLNRMEHEGFSSALIRGITVSAEKAAGLYCDKKNG"
     gene            3310..>4470
                     /locus_tag="P5219_01325"
     CDS             3310..>4470
                     /locus_tag="P5219_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HRX25427.1"
                     /translation="MVKILEQGAWLVDGRHIVEEGPGAERKAAELAGRPVSRDEARKG
                     TIAWSILESHNTSGSMERLKIQFDALTSHDITYVSIIQTARASGLERFPLPYVLTNCH
                     NSLCAVGGTINEDDHLFGLSAARKYGGIYVPAHMAVIHMYMREAMAGCGKMILGSDSH
                     TRYGALGTLAVGEGGGELVKQLLAQTYDIAYPRVVGVYLTGKPCPGVGPQDVALAVIG
                     AVFSRGYVKNSVMEFVGDGVSNLSADYRCGVDVMTTETACLSSIWRTDGNVKDFLAAH
                     GRPDDYRELNPGKTAWYDCMIMVDLSAVKPMIALPFHPSNAMEIERFNENPGDILREV
                     EQSAAEILGNTKARFSLTGKLEKGRLRVDQGIIAGCSGGTYTNVMAAASLLKG"
BASE COUNT          933 a         1408 c         1411 g          718 t
ORIGIN      
        1 aacgccgcca cccgcacggc agccctgctg accggcgaag tggacatcat cgaggacgtt
       61 cccgtccgtg acgtggacaa gctgaagaac acggaaggcc tttccgtggt ggaccggccc
      121 agcgagcggc tgatctatct ccacgtggac tcccatcgtc cccagggccc cggcatcatc
      181 gggctcgaca agaaccccct cgcggacctc agggttcgga aagccctgtc tctggccatc
      241 aaccgggatt ccatcgtgaa gatgatcatg aacggcaacg cctacgccac gaaccagatg
      301 gtcctgaagg gccggagagg atacgtggag gacatgcccg ccccgaagta cgaccccgaa
      361 gaggcgaaga agctcctcgc cgaggccggc tatcccgagg gcttcaaagt gtaccttgac
      421 gctcccaacg gccggtaccc caacgacggc caggtcgccc aggcgctcgc cagccagctc
      481 accaaggctg gaatcctgat tgaactccgc cttcacccca agaccacctt cttcgatttc
      541 gtccgccccg gcgacaagtc cagccttgtc atgaccggat ggtccgagcc catcgacgtg
      601 ggcgagatgg ccagcgttct cttctacacc aggggcaaga atcccgccaa gggcggatcc
      661 aaccggggcc actacgccaa ccccgggttt gacgccctcg tcgacgaagc cgacggcacg
      721 gcggaccccg ggaagcgcag ggtcatcctc gagaaggccg cccgcatgat cgtggaggac
      781 ggcggcatcg tccccctgta cttccagcag gacctctacg gcaagaagga cgccgtggtg
      841 ttcgagccca gggcggacaa gtccatcctg gcctacgaca tggacgtgaa atagccggat
      901 tttccacgcc gggcaagttg caggcaggcc ggggcccagt gccgccggcc tgcctttttt
      961 caaaagaagg agagattctt ttcatgaaag accgcatcat aaacagggtt gaagaaatag
     1021 ggaaggaact gctccagctg agccgcgaca tccacggatg ccccgagacg gccttcgagg
     1081 agcggaaggc ggcccggtgg cagaaggagc tgctggagcg gcacggtttc accgtggaaa
     1141 tgcccttctg cgccatggaa accgccttcc gggcggtgaa gaagggacgg gaggaaggac
     1201 ccacagtggc attcctctcg gagtacgacg cactgaaggg cctcggtcac gcctgcggcc
     1261 acaacatcat cgccgcgtcc tccgtggggg cggggatcgc cctgggatcc gtcctggagg
     1321 acattgcggg tgaagtccag gtcatcggaa cccccggcga ggaggaagga gggggaaaaa
     1381 tctacatggt ggagcgggga tgcttcgccg gcgtggattt cgccctcatg gtgcacccca
     1441 acacgaagaa tctcatcggc aggggcggac ttgccgccca gagcgtgaga atcgagtttc
     1501 tcggaagggc ggcccactca gccgttcccg acaagggcgt gaacgccctc acgtccctca
     1561 tcgccttctt caacggcatt gacaccctcc ggcagacctg gccgaactcc gccaagatca
     1621 acggcatcat caccaggggc ggcacggctt ccaacgtcat ccccgacctc gccgaggcct
     1681 gctttaccgt ccgggcgtcc acgaaaaagg agctcaacgc catgtacagg gatctgaacc
     1741 gggtcgccga ggccgcggcc atggtcacgg gggcgtcctt tctcctggag ggggagcccg
     1801 tgtatgccga gcggtatccc aacagcgcca tgggcgaggc cttcaagaga aacatggagt
     1861 ccctcggtga gaccatggaa tatcccgacc cccgggagcg gttcgggtcg tcggacgtgg
     1921 ggaatgtttc cctggagctt cccaccatcc atgagtacct tgccatcgcg gacacgtccg
     1981 tggccggaca cacggttgaa ttccgggagg ccgccgtctc ccccaggggc ggcgaggtgg
     2041 tccttctcgc cgccgaaggt ctcgctctta caggctggga tcttctcacc gacggggatc
     2101 tgcgggaagc ggcccgggag gaataccggc ggaaggtcct gcccttcagg gactgacggg
     2161 aatctccggg cagcggggag cgtcaccggt gctcgcttgg gccgtcggtg cagattgtcg
     2221 tccatgacaa ctgcacctta aaccgacatc catgtcggtt tcccggcccg ggcgcttttc
     2281 ccggcgacac tccctcttcc caaaaaatgc cgattcagcc tgaggattcg gccttttcaa
     2341 caggggctga ctttgatata ctgtccgcta aaaaattacg gctcaccgga tctcccgatg
     2401 atgtccgcaa atttaaggga ggttcaaaca gaaatggaaa acacggtacg gattgccatt
     2461 ctcggagccg ggaacatcgg aacgagcatc gccaggggcc tggcccaggc cgggaatttc
     2521 accccgggaa tgatcaccct tacccggagg cggacccacc tcctggagga gtacaaggcc
     2581 atgggcttcg aggtgaccag cgacaacccg gcggcggtga aaggggcgga cgtggtgatc
     2641 gtggccgtgg aaccccaaag ggtggacact ctcctccagg aaattgcccc ggcgctggat
     2701 tttgaccgcc atatcctcat ctccgtagtc acggggcttg ccatcaaaag aatcgaggag
     2761 gtcatcggga agcctctccg gatcgccagg gccatgccga atacggccat cgccgtccgg
     2821 gagtccatga cctgcctcgc ctccaacggt cccgacgacc gggcgctgga gatggccaag
     2881 cggatcttca acgacgtggg gaagaccctc cggatcaggg aagaggacat gatcgccgcc
     2941 acagccctgg gcgcctgcgg catagccttt ttcctccggg ccattcgggc tgcctcccag
     3001 ggggggatcg aaatcggcct gtcggccaag aactcactgg ccatggcggt ccagacggcc
     3061 aagggcgccg cctgcctgct ctccgtgagc gggagccacc ccgaaaatga aatcgacaag
     3121 gtgaccaccc ccaagggctg caccatttcc ggtctcaacc ggatggaaca cgaggggttc
     3181 agctccgccc tcatccgggg gatcacagtg tccgccgaaa aggcggcggg actgtactgc
     3241 gacaagaaga acggctgagc cgaagccgga cctgcgaacg gtgaaccgga acgacggagg
     3301 ggatatggta tggtgaagat actcgaacag ggagcctggc tcgtggacgg aaggcacatc
     3361 gtggaggagg gacccggcgc ggaaaggaag gccgccgaac tggccggcag gcccgtcagc
     3421 cgtgatgagg cccggaaggg caccattgcc tggtccatcc tggagagcca caacacctca
     3481 ggctccatgg agcggctgaa gatacaattc gatgccctga cctcccacga catcacctac
     3541 gtgagcatca tccagaccgc ccgggcaagc ggcctggaga ggttccccct cccctacgtc
     3601 ctcacgaact gccacaattc gctgtgcgcc gtggggggga ccatcaacga ggacgaccac
     3661 ctcttcggcc tctcggcggc gcgaaaatac ggaggcatct acgttcccgc ccacatggcg
     3721 gtgattcaca tgtacatgcg ggaggccatg gcgggctgcg gaaaaatgat cctcgggagc
     3781 gacagccaca cccgatacgg cgccctggga acccttgccg tcggagaggg cggaggagag
     3841 ctggtaaagc agctcctggc gcagacctac gacatcgcct acccccgggt ggtgggggtg
     3901 tacctcacgg gaaaaccctg tcccggagtc ggtccccagg acgtggcgct ggcagtgatc
     3961 ggcgccgtct tcagccgggg ctatgtcaag aacagcgtca tggagttcgt cggggacggc
     4021 gtttcgaacc tctccgccga ttaccggtgc ggcgtggatg tgatgaccac cgagacggcc
     4081 tgtctttcct cgatctggcg caccgacggt aacgtgaaag acttccttgc agcccacggg
     4141 cggccggatg attaccggga gctgaacccc ggcaaaaccg catggtacga ctgcatgatc
     4201 atggtggacc tttctgcggt gaagcccatg atcgccctgc cgttccatcc ctccaacgcc
     4261 atggagattg aaaggttcaa cgagaacccg ggcgacatcc tcagggaagt ggaacaatcc
     4321 gcagcggaaa ttctgggaaa cacgaaggcc aggttcagcc tcaccgggaa gctcgagaag
     4381 ggcaggctca gggtggacca ggggatcatt gcgggatgtt ccgggggcac ctacacgaac
     4441 gtcatggcgg cggcgtccct cctgaaggga
//
LOCUS       DAOTHD010000042         4469 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_23099_length_4469_cov_2.853194, whole genome shotgun sequence.
ACCESSION   DAOTHD010000042 DAOTHD010000000
VERSION     DAOTHD010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23099_length_4469_cov_2.853194"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="P5219_01330"
     CDS             <1..478
                     /locus_tag="P5219_01330"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRX25428.1"
                     /translation="RKDNTGYDLRDLFIGAEGTLGIITKVIVNLEPKPGKTVNLLVAF
                     PDLDTAVDSVAKVVRTGISVISCELMDKLSVNIAAPHVNTELPYQDRADAFLLIQIEG
                     DSDERLEEGYEKVGTLCLENGALEVFVAESRTESGMIWNIRQNLAEAMRAMIHTAP"
     gene            487..852
                     /locus_tag="P5219_01335"
     CDS             487..852
                     /locus_tag="P5219_01335"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRX25429.1"
                     /translation="MVVPLSTVPEMVEEIRKAGKERGITVGILGHIADGNLHPIIFKP
                     AGMEPMEWAEYAEAFYDDLTSVAIRLGGVGSGEHGIGYVKMPIFLHSKPAEEIEIMRG
                     IKRSFDPDGILNPGKLLGS"
     gene            930..2051
                     /locus_tag="P5219_01340"
     CDS             930..2051
                     /locus_tag="P5219_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25430.1"
                     /translation="MKSKSDMGILAVAFVWFTTHFGGGFASGRQLVDFFVSYGWYALI
                     TPVLSIAIVTAVLYYAWSFAVTYKTFDYRAWGNQYFKPFEAIFSNAYEVMYILILLIA
                     SAVAFATGGTVMKQILGTPYVMNTVIVAVFIFFLTIFGAEMVRRAASTMAVIIIAGMT
                     FIYVANLVTNFPKLLEVIRTAPSPKGFVEAFWASMKYAGFQACAMGAYIAVADALTTR
                     EDARKTSVWGFLINAGVLWLATAGVLLHYPAILQESVPVLYVAAHGGGGAFGTAIVSL
                     LIFLAVISTGVSLIFGGARRIVAFWGRTRGSSVAGDRKVNIAASLIYVLITWGIALFG
                     LIPLVAKGYGYMGTLSFPLLILPVVLIGLLRSKDWGKNA"
     gene            2251..3768
                     /locus_tag="P5219_01345"
     CDS             2251..3768
                     /locus_tag="P5219_01345"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRX25431.1"
                     /translation="MKSKYRLLSALMILLSCSAAAFFFSAFDRQIETVYGDNTRDAIF
                     EIKKTFLQHTVQNQIARIDAARQRHAGLAIARLEEKSALLETFPSGNREEFITFFREC
                     FRDGTVWTAALWDPAAGAVLHDPKGLLRKGFPGTPDGGEFRDFSAWKIVDFRGLRAFF
                     GISRAAADEAVKKEIASEIHASRFPFDSYIWVNEVVNFDGGLNYAIRRVHPNLRDTEG
                     AFLSTETRDVKGNRPYLTELEGVKKDGRIFFTYFFKRKDSEEISEKLTYAELYRDYNW
                     IIAMGIHLDDIFASIGEANEESGTTSGRLIVLFLLLMVALLGISDLILLFLEGKVHGE
                     SRKGLEDELNRDPLTGAFSRRAGETEMARAFHSFTKGALPPAIVLFDIDRFKEVNDTF
                     GHDAGDAVLKEVVKTVLRNVRTTDRLFRWGGDEFLLVAEGLKAENALPVAENIVKRVS
                     EHAVSVGGHSIPVTISVGIACFRDGDRGYEDALKRADRALYRSKSGGRNQANMEE"
     gene            3840..4352
                     /gene="tpx"
                     /locus_tag="P5219_01350"
     CDS             3840..4352
                     /gene="tpx"
                     /locus_tag="P5219_01350"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HRX25432.1"
                     /translation="MERKGITTMKGNPMTLVGPELKAGDKAPDFTVLDQAMAPKSLKD
                     FEGKFKVISVTPSLDTPVCDLQIHWFNEDAANQPADVAVLNISMDLPFAIKRFCATGG
                     IDRAEALSDHRDASFGTNWGVLMKELRLLARSIFVVDRENVIRYVQIVPEQTTEPDYE
                     AALNALKSLM"
BASE COUNT          957 a         1345 c         1300 g          867 t
ORIGIN      
        1 caggaaggac aatacgggct acgatctgcg cgacctgttc atcggtgccg agggaaccct
       61 gggcatcatc accaaggtca tcgtcaacct cgaacccaag cccgggaaaa cggtgaacct
      121 cctggtggcc ttcccggacc tggatacggc ggtggacagc gtggcgaagg tggtccgcac
      181 ggggatcagc gtcatctcct gcgagctgat ggacaagctc tcggtgaata tcgccgctcc
      241 ccacgtgaac acggagctcc cctaccagga cagggccgac gccttcctcc tcatccagat
      301 cgagggagac tcggacgaac ggctcgagga agggtacgag aaggtcggga ccctctgcct
      361 cgagaacggg gcgctggagg ttttcgtggc ggagagccgg accgagtcgg gcatgatctg
      421 gaacatacgg cagaacctcg ccgaggccat gcgggccatg atccatactg ctccctgagc
      481 ggcgacatgg tggttcccct gtccaccgtc cccgagatgg ttgaggagat ccggaaggcg
      541 ggaaaggaac ggggcatcac cgtgggcatc ctcggccaca tcgccgacgg gaacctgcac
      601 cccatcatct tcaagcccgc aggcatggaa cccatggaat gggcggagta cgccgaggcc
      661 ttctacgacg acctcacatc agtagcgata cgcctcggcg gcgtgggcag cggagagcac
      721 ggcatcggct acgtgaagat gcccatcttc ctccactcca agccggcgga ggaaatagag
      781 atcatgagag gaatcaagcg gtccttcgac cccgacggga tcctgaaccc cgggaaactg
      841 ctcggcagct gacaggtgtt ccggggcccg gatccggacc ccggccgggg aagttatgaa
      901 caggagacac gagaggagca gggaacggca tgaaatcgaa gagtgacatg ggaattctgg
      961 ctgtggcctt tgtctggttc accacccatt tcggcggcgg cttcgccagc gggagacagc
     1021 tcgttgattt tttcgtatcc tacggatggt atgccctgat cacccccgtc ctgtccattg
     1081 ccatcgtcac ggcggtgctc tactatgcgt ggagcttcgc cgtaacctac aaaacgttcg
     1141 actacagggc atggggcaac cagtatttca aacccttcga ggccatcttc tccaacgcct
     1201 acgaagtcat gtacatcctt atccttctca tcgcgtcggc ggtggccttc gccaccggag
     1261 gcacggtgat gaagcagatc ctcgggacac cctacgtaat gaacacggtg atcgtggcgg
     1321 tcttcatctt cttcctgacc atcttcggcg cggagatggt gcggagggcc gcctccacca
     1381 tggcggtcat catcattgcg gggatgacct tcatttacgt ggcgaacctc gtgacaaact
     1441 tccccaagct gcttgaggtc atccgcacag ccccgtcccc gaagggcttt gtggaggctt
     1501 tctgggcatc catgaagtat gccggtttcc aggcctgcgc catgggggcc tacatcgccg
     1561 tggccgatgc cctgacgacc agggaggacg cccggaaaac atccgtctgg ggctttctca
     1621 tcaacgcggg agtactctgg cttgccaccg cgggggtgct tctccactat ccggccatcc
     1681 tccaggaaag cgtgcccgtg ctgtacgtgg cggcccacgg cggaggcggc gctttcggca
     1741 cggccatcgt gtccctcctg attttcctgg cagtcatctc caccggcgtg agcctcatct
     1801 tcggcggcgc caggcgcatc gtggccttct ggggaagaac ccggggctcc tccgtcgccg
     1861 gcgacaggaa ggtgaacatc gccgcatccc tcatctatgt cctcatcacc tggggcatcg
     1921 ccctgttcgg cctcatcccg ctggttgcca aggggtacgg ctacatggga accctttcct
     1981 tcccgctgct catcctgccg gtcgtcctca tcggcctgct ccggtcgaaa gactggggaa
     2041 agaacgcctg aaaatctttc gggggagggg ggcaccctgt cctctctccc ctcctttcct
     2101 tccgcatcca tccgccccat attcaaaacc atctccggca cagttccctt gaccttcggg
     2161 aaaaaagcgg gtacattctc tgaagccggc cggttataac cagaagagca gaccggacac
     2221 accgctgacg ggaaaggaag gagagccgca gtgaaaagca aataccgcct tctgagcgcc
     2281 ctgatgattc ttctgtcctg ctcggcagcc gcttttttct tcagtgcctt cgaccgtcaa
     2341 atagaaaccg tttatggtga caacaccagg gatgccatct tcgaaataaa gaaaaccttc
     2401 ctccagcaca ctgtgcagaa ccagatcgcc aggatcgacg cggcaagaca gcgccatgca
     2461 ggccttgcca tcgcaaggct cgaagagaaa agcgcccttc tggagacttt tccatccgga
     2521 aacagagagg agttcatcac ctttttccgg gagtgcttcc gggacggcac tgtctggacg
     2581 gccgccctgt gggacccggc cgccggtgca gtgctccatg acccgaaggg cctgctccgg
     2641 aaggggtttc ccggtacgcc ggacggaggg gagttcaggg atttttcggc ctggaagata
     2701 gttgatttca gagggctgcg ggcttttttc ggcatttccc gggcagcggc cgacgaggcc
     2761 gtgaagaaag agatcgcctc ggagattcat gcctcccgtt tccccttcga ctcctacatc
     2821 tgggtcaacg aggtggtgaa cttcgacggc ggactgaact acgccatccg gagagtccac
     2881 ccgaacctga gggacacgga aggagcgttt ctctccaccg aaaccaggga cgtgaaggga
     2941 aaccgtccgt acctgaccga actggaggga gtgaagaagg acgggcgtat ttttttcact
     3001 tacttcttca agagaaagga cagcgaagaa atctccgaaa agctgaccta tgctgagctg
     3061 taccgggact acaactggat catcgccatg gggatccatc tcgacgacat cttcgcatcc
     3121 atcggcgagg ccaacgagga aagcggcacg acctccgggc gcctcatcgt tctcttcctt
     3181 cttctcatgg tcgcgctgct gggaatcagc gaccttatcc tccttttcct cgagggcaaa
     3241 gtccacggag aatccagaaa gggcctcgag gatgaactga acagggaccc cctgacgggg
     3301 gccttctccc gcagggccgg agagacggaa atggcacggg ctttccactc tttcacgaaa
     3361 ggtgcattgc cgccggcgat tgtcctcttt gacatcgacc gcttcaagga agtgaatgat
     3421 accttcggcc acgacgcggg ggacgcagta ctgaaagagg tcgtcaagac ggtcctgaga
     3481 aatgtaagaa ccaccgaccg gcttttccgg tggggcggcg acgaatttct cctggtggcc
     3541 gaaggactta aggcggagaa cgccctcccg gtagccgaga acattgtgaa gagggtttca
     3601 gaacatgcag tctccgtcgg cggacactcc attcctgtca ccatctccgt tggcatagcc
     3661 tgcttccgcg acggtgacag gggatatgaa gacgccctga aacgggcgga cagggcgctg
     3721 taccggtcga aatcaggggg aaggaaccag gcgaatatgg aagagtaaca tcttgacctt
     3781 tcctgatccg tcatggtaga ctcatctaac aaagattcta tttcggaggt gaatatatca
     3841 tggaaagaaa aggaataacc acaatgaagg gaaaccccat gacccttgtg ggtcccgaat
     3901 tgaaggccgg agacaaggct cccgacttta cagtgcttga tcaggctatg gccccgaagt
     3961 ccctgaagga cttcgagggc aaattcaagg tgatctcggt cactccgtcc ctggacactc
     4021 ccgtatgcga cctccagatc cactggttca acgaggatgc ggccaaccag ccggcagacg
     4081 tggctgtgct gaacatttcc atggacctgc ccttcgccat aaagcggttc tgcgccaccg
     4141 gaggcatcga cagggccgaa gccctttccg accacaggga cgcctccttc ggaaccaact
     4201 ggggcgtgct gatgaaggaa ctccggctcc ttgcacggtc catcttcgtg gtggacaggg
     4261 agaacgttat ccggtacgtc cagatcgtcc cggaacagac cacggaaccc gattacgaag
     4321 cggccctgaa cgccctgaaa tcgctgatgt agaacatctt cccccaccgg caataaacgg
     4381 cgggacccgg agaaaaccgg gtcccgccgt ttattgcttc gttccggaac cctgtcatcc
     4441 ttccgtgctg agaagcttcc tggccacgt
//
LOCUS       DAOTHD010000043         4459 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_23191_length_4459_cov_3.225023, whole genome shotgun sequence.
ACCESSION   DAOTHD010000043 DAOTHD010000000
VERSION     DAOTHD010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23191_length_4459_cov_3.225023"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..146
                     /locus_tag="P5219_01355"
     tRNA            70..146
                     /locus_tag="P5219_01355"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:104..106,aa:Arg,seq:acg)
     gene            369..2168
                     /locus_tag="P5219_01360"
     CDS             369..2168
                     /locus_tag="P5219_01360"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HRX25433.1"
                     /translation="MTLRNTPCSDHRPYSSRTLKKSPLALLLLLALLFAGEAFGAQTV
                     RKDAFITQLFQARGFAAPAGENDMVRAALELDLIPVPEGRLDAPITMKEAIVFAVHSL
                     GLASVADVLSGAPLPFRDIGRLKPLERGYLAAALNMNPPLLRKGVTSFGPSKKLTPKE
                     ARNIAAIVRAARKGLSLSVKYSPMKGMTVQVNREGVHDRPPKWRAAVNGFDSREEAEF
                     FRDALAEKGVEGTVDSQNFDWRVRSALSETYGPIRGFLVACESLGRKGVVFASPASWD
                     TQGAPRFWVMVILDPARFDIRPVFAPEGLYALAPLTSMTTGAAAAVNGGYFSISGKER
                     GAPIGAVIERGVMVNPPYKGRTILGWNGKNQAAFGQMDWRAEVHFPGGGFMDVTGLNR
                     TVKGDGVILFTRHFGECTPVFSGPVVEAVLDGDTCVEVRREGGNPIPPGKRVLAVYGT
                     PARFAESVTPGDRVRIVQTVNGGDPYWTSMTNAVQGGPFLVRKGVLSMETENLNDSIV
                     NKRHPRSVIGLTGKGQWFFFVGDGRNAVHSVGFTLAETAEILKKNGVSYALNLDGGGS
                     STLYAGGRIMNVLSDGRERPVSYGVGAFPKGGK"
     gene            2284..2781
                     /locus_tag="P5219_01365"
     CDS             2284..2781
                     /locus_tag="P5219_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25434.1"
                     /translation="MKKQSRLVTLFVLALLALVLSISAAFASAAPQPVNFGQDANKMQ
                     WYLVNYGKNDEGPFACTRKYYTNPDEKQKTIDLIVEKFAIDKEAASGLYFTEYGYIYS
                     ADGKQFAVTYLNHYDMVGNVIKSTEYAAADREFVGMSKGMIPLKAHPYAIGKLPAKKA
                     ASRKK"
     gene            2890..3081
                     /locus_tag="P5219_01370"
     CDS             2890..3081
                     /locus_tag="P5219_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase family protein"
                     /protein_id="HRX25435.1"
                     /translation="MPIVNIYMYEGRSVDQKRKLAAEVTDAICRALDVGPEVVRIMIN
                     DMPRENMAVAGVLNIDKGK"
     gene            3103..3393
                     /locus_tag="P5219_01375"
     CDS             3103..3393
                     /locus_tag="P5219_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25436.1"
                     /translation="MNKFLKALRTGWEFFVLFHHGTFVDKNMAVVRKEAFDINDNLML
                     LLFGDYLGIPNPMSYYMLELLPYVADDLEPWERRMQNRKFIIAEKAAQYDFD"
     gene            3407..4345
                     /locus_tag="P5219_01380"
     CDS             3407..4345
                     /locus_tag="P5219_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HRX25437.1"
                     /translation="MRHFTFFGGKGGTGKTSCAASYALSLAARGVRTLVVSTDPAHSL
                     ADAFDKPIGFQTAPLCPNLWGLEIDAEVEAKKYMKSIQDKMLDLVSAAIVDEIKRQIE
                     IAYMSPGAEEAAIFDKFIELMEAVGRDYDAIVFDTAPTGHTLRLLTLPEILGAWIEHL
                     IEKRRKAMDLMKMAARYDKPLAERIANDPIIQTLTARREKFALARKYLTDPEASIFYF
                     VLNPEKLPILETARAVELLSKHGIAVGGVVVNKVIPPDAGPFLEKKRLSQEGYLREIR
                     GRFSSMTIIELPMLDDDIQGMVQLQRISAMMEGLDS"
BASE COUNT         1011 a         1267 c         1269 g          912 t
ORIGIN      
        1 gcgagtaggg gggcttaaac ctccctcgag ggttcgaatc cctcacccac cgccatcaca
       61 aaccgacagg cgccggtagc tcagatggat agagcggcgg tctacggaac cgcaggtcac
      121 gagttcgaat cttgtccggc gcgccactga aagaccagcc cccgcagtct ttgcgggggt
      181 ttttttgtag acgtcacgag ttattgcacg gccggaaaat gccccggcat accggaatga
      241 aaaggaacgt attgctccaa tccgcctttt tccgtatacc ctcctcttct ttcgtctgtt
      301 atacttcctt gcgaatccgg ctgaaatgaa cccgtctgaa gagagcagtt cattcaagga
      361 gaatgcccat gaccctcaga aataccccat gttccgacca ccgtccctat tcgtcccgca
      421 ctttgaagaa gagtcccctt gctttgctcc ttctcctggc ccttctgttt gccggggaag
      481 ctttcggagc ccagaccgtt cgaaaggatg cctttataac ccagcttttc caggcgaggg
      541 gcttcgcagc tcccgccggg gaaaatgaca tggtcagggc ggccctcgag ctcgatttga
      601 tccctgttcc ggagggaagg ctcgatgccc cgatcaccat gaaggaagcc atcgtgtttg
      661 ccgttcacag cctgggcctt gcctccgttg ccgatgttct ctcgggagcg cccctgccct
      721 tccgggatat aggaaggctc aagcccctgg aaagaggata cctggcggca gccctgaaca
      781 tgaatccgcc cctgctgagg aaaggggtca cctccttcgg tccctcgaaa aagctgactc
      841 cgaaggaagc ccggaatatt gccgccatcg tccgggcagc ccggaaaggg ctgtctctct
      901 ccgtaaaata ttccccgatg aaggggatga cggtgcaagt gaacagggag ggggtccatg
      961 accgtccccc gaaatggcgg gctgccgtca acggtttcga ctccagggag gaggcggaat
     1021 ttttccggga cgccctggcc gaaaagggcg tggagggaac ggtggacagc cagaattttg
     1081 actggcgggt ccggtctgct ctttccgaga cctacggtcc cattcgggga ttccttgtcg
     1141 cctgcgaaag tctcggaaga aagggcgtgg tctttgcctc tcccgcgagc tgggacacac
     1201 agggggctcc aagattctgg gtcatggtca tactcgatcc cgcccggttc gacatccggc
     1261 ccgtctttgc cccggaaggc ctctatgccc tcgcccccct cacctcaatg accacgggag
     1321 ctgcggcggc cgtcaacggc ggctatttca gcatcagcgg caaggagcgt ggagcaccca
     1381 tcggtgccgt catcgaaaga ggcgtcatgg tgaaccctcc ttataaagga cggaccattc
     1441 tcggctggaa cgggaagaac caggcagcct tcggccagat ggactggaga gcggaagtgc
     1501 attttccggg agggggcttc atggacgtca ccggactgaa cagaacagtc aagggcgacg
     1561 gggtgatact cttcaccagg cacttcggcg aatgtacccc cgtgttttcc ggtcccgtgg
     1621 tggaagctgt ccttgacggc gatacctgcg tggaggtccg gcgggaggga gggaatccca
     1681 ttccgccggg gaagcgggtc cttgccgtct acggtacgcc cgcgaggttt gccgaatcag
     1741 tcactcccgg agacagggtg cggattgtcc agacggtcaa tggaggcgac ccctactgga
     1801 catcaatgac caacgctgtc cagggaggtc ccttccttgt gcggaagggg gttctttcca
     1861 tggaaacgga aaacctcaac gattccatag tgaacaagcg ccatccccgg tcggtcatcg
     1921 gcctcaccgg caaggggcag tggtttttct tcgtcggaga cggccgcaac gcggtccaca
     1981 gcgtgggatt cacccttgcg gaaacggcgg agatcttgaa gaaaaacggg gtatcctacg
     2041 cactgaacct ggacggcggc ggctcttcaa ccctctacgc gggagggagg atcatgaacg
     2101 ttctttccga tggaagggaa aggccggtaa gttacggcgt cggggctttt ccgaagggag
     2161 ggaaataacg ggaataactg aaaataggaa taatcctgtg gagaagaacc gaatacgccg
     2221 ttttatgata gactacacga cgcaagaaaa agaaaaaaat acggaaggag ttgtaccagt
     2281 caaatgaaaa aacagtcccg tctcgtcacc ctttttgtcc tcgcgcttct cgcccttgtc
     2341 ctctcgattt ccgccgcttt cgcctccgca gcaccccagc cggtgaactt cggccaggat
     2401 gccaacaaaa tgcagtggta ccttgtgaac tacggcaaga acgacgaggg cccctttgca
     2461 tgtaccagga agtattacac gaaccctgat gaaaagcaga agaccattga tctcatcgtc
     2521 gaaaagttcg ccatcgacaa ggaagccgct tccggcctgt acttcacaga atacggctac
     2581 atctattccg cggacggcaa gcagtttgca gtaacctacc tcaaccacta cgacatggtt
     2641 ggcaacgtga tcaagtccac tgaatacgcc gccgccgaca gggaattcgt cgggatgtcc
     2701 aaggggatga tccctctgaa ggctcatccc tacgctatcg gcaaacttcc tgcgaagaag
     2761 gcggcctcca gaaagaagta gtttcaccgc agaccaagcc cggccccgag ccgggctttt
     2821 tttatgcctt ccgttgcgtt tcgcccggaa atacagtaat cttcaataaa agaagaggag
     2881 gcggtttcca tgcccatagt caatatctac atgtacgaag gacgttcagt ggaccagaaa
     2941 agaaaactcg ccgcagaggt gaccgatgcg atctgcaggg ctcttgatgt ggggcccgag
     3001 gtagtcagga tcatgatcaa cgacatgccc agggaaaata tggcggttgc cggagtactg
     3061 aacatcgaca aggggaaata aacccttccg ggcaggcaga ccatgaacaa attcctgaaa
     3121 gcgctccgta cggggtggga gtttttcgtt ctcttccatc atggtacatt cgtggataaa
     3181 aatatggccg tcgtccgaaa agaggccttc gacatcaacg acaatctcat gctgcttctg
     3241 ttcggcgatt atctcggcat cccgaatccc atgtcctatt acatgctcga gcttcttccc
     3301 tatgtggcgg acgacctgga gccatgggaa cggaggatgc agaacagaaa gttcatcatt
     3361 gccgaaaaag cggcccaata cgatttcgac tgaggtgaag catacaatga ggcactttac
     3421 gttctttgga ggaaagggcg gaacaggaaa gacctcctgc gcggcttcct atgctctttc
     3481 gctggcggcg cggggtgtgc gtaccctggt ggtatcaacc gacccggccc actcccttgc
     3541 cgatgccttc gacaagccca tcggcttcca gacggcgccc ctgtgcccga acctttgggg
     3601 tctggagatc gacgccgagg tggaagcaaa gaagtacatg aaatcgatcc aggacaagat
     3661 gctcgacctg gtcagcgccg ccattgtgga cgaaatcaag cggcagatcg agattgccta
     3721 catgtctccc ggggccgaag aggcggccat tttcgacaag ttcatcgagc tcatggaagc
     3781 ggtgggcagg gattatgacg ccattgtctt cgataccgcc cccaccggcc atactctccg
     3841 gcttttgacc cttcccgaaa tcctcggtgc ctggatcgag catctcatcg agaagcggcg
     3901 gaaagccatg gaccttatga agatggcggc caggtacgat aagccgctgg cagagcggat
     3961 agccaacgat cccatcatac agacccttac cgccagaagg gagaaattcg ccctggcgag
     4021 gaaataccta actgaccctg aagcctcgat cttttacttc gtgctgaatc cggagaagct
     4081 tcccatcctg gaaacggccc gggccgtgga gctgctatcg aagcacggca tcgccgtggg
     4141 gggcgtggtg gtgaacaagg ttattcctcc tgacgccgga ccttttctcg agaaaaaacg
     4201 gctttcccag gaaggatacc tccgggagat ccgcggccgc ttttcgtcca tgaccatcat
     4261 cgagcttccc atgctggacg acgatatcca gggaatggtc cagcttcagc ggatttccgc
     4321 catgatggag ggactggatt catgatcacc gacaccctgg acaggctcgg tgcgtatttt
     4381 tgccttgggg gaagcctcgg aaaggccctt gagttcctcg cgtccagatc cctggagtcc
     4441 cttccggaag gtcgtacgg
//
LOCUS       DAOTHD010000044         4450 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_23246_length_4450_cov_3.253015, whole genome shotgun sequence.
ACCESSION   DAOTHD010000044 DAOTHD010000000
VERSION     DAOTHD010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23246_length_4450_cov_3.253015"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..603)
                     /locus_tag="P5219_01385"
     CDS             complement(136..603)
                     /locus_tag="P5219_01385"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRX25438.1"
                     /translation="MKKANYCIAAAFLLLAAFVLYSAANFEQTLIQDDYVGAAFFPEL
                     LAWMTGGLALFLGGLTFRGKFDGDGRTLSDLFPGEIRIALAGLAVITLYVLSLDYFGF
                     ILATAALNFALLLLFGVRNPLSLAVFPAAISLAVYGVFYKLLVVPLPEGLFYF"
     gene            complement(692..1633)
                     /locus_tag="P5219_01390"
     CDS             complement(692..1633)
                     /locus_tag="P5219_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRX25439.1"
                     /translation="MKKAALVLALASLIFGSAAFAADFPAKNIRLLVPFAAGGGTDAV
                     ARALAASAEKHLGQPVVVMNKAGGSGAVGMTEGAMAKADGYTVTMVTREIAWLPQMGL
                     AQITPDNFDLLALINEDPAIMLVRPDSKFASVKDVLEAAKAAPGSVKFGSTAKPNFYL
                     LALELNQKVKFNQIPYNGAAEAIPALMGGHVDFTMVNPGEAISQIKAGQLKPLGVASD
                     VRMAGLPEVPTMTEQGYPIVTGTWRGLALPKGTPADVKAVLADAFQKAASEKEFTGFM
                     NGRTLGIRFMKSEEFKTFVDNDVKALTDIVEEVKKQQ"
     gene            complement(1696..2457)
                     /gene="kduD"
                     /locus_tag="P5219_01395"
     CDS             complement(1696..2457)
                     /gene="kduD"
                     /locus_tag="P5219_01395"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008678 - 2-deoxy-D-gluconate
                     3-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HRX25440.1"
                     /translation="MILDSFRLDGKVAVVTGARKGLGFGIAEGLAEAGADIVGVGPIP
                     MPETEKAVTSLGRKFLYVEADLTSQERIPDIVAETVAGLGSLDILVNNAGIIRRADIL
                     EFSEKDWDDVMNVNLKSLFFLSQEAARQMEKQGRGGKIVNIASMLSFQGGIRVPSYTA
                     SKSAVGGLTKLLANELAKFGIQVNAIAPGYMATDNTEALQKDPVRNKEILARIPVGRW
                     GTPDDLKGAAVFLSSAASDYVTGYIVAVDGGWLSR"
     gene            complement(2489..2977)
                     /locus_tag="P5219_01400"
     CDS             complement(2489..2977)
                     /locus_tag="P5219_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRX25441.1"
                     /translation="MLQAMKLAGAEVLITDFADSRLKAAETLGADKTVNGRTGDLAAA
                     LADFTGEFGADVVVEAVGMPRLLEESLGYAAPGGRIVVLGFHPEPAKIPEVIVVKKEL
                     KIVGSRMNCRRFPQVIEWFEKGQVNPEPLISAVYPFEKIDTAFREILEDPEKYLKVLI
                     TY"
     gene            complement(3020..3502)
                     /locus_tag="P5219_01405"
     CDS             complement(3020..3502)
                     /locus_tag="P5219_01405"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRX25442.1"
                     /translation="MRSAKLVAPKQIRIFEDRMPLPTEGEVLVRVKAAGICGSDLHIY
                     RGERSDVALPRILGHELVGEVESVGSGVTRFSRGDRVTVDPVVSCGKCVSCRRGYDNL
                     CSSVKCLGVQVDGGYCDYIAVPEEKVYSLPLGLAWEKAALIEPFSIGAEVLARSEVRE
                     "
     gene            complement(3755..>4450)
                     /locus_tag="P5219_01410"
     CDS             complement(3755..>4450)
                     /locus_tag="P5219_01410"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRX25443.1"
                     /translation="GQFVPTMDVLSLPYVFQSTDHMFKALNGKAGEMIKADIEKAGFV
                     FLYWGDGGSRNLINNKRPITKPEDLKGLKIRVMDSKLMVNTLNAMGAIATPMAQGEVY
                     SALQQGVLDGWENNPVTLLTLKLYEVSDIFSWTRHFMVPDLVIISKQAFAKLTPEQQK
                     ALVEAGHEAEAKQREYWNAFVGKTVEELKTHNVKFNEVDVAPFVQAVQPVWEEYKAKF
                     GTELLDLIIAANK"
BASE COUNT          851 a         1344 c         1341 g          914 t
ORIGIN      
        1 cgagcgtggc ggtgaggccc ggcagggcgc cgatgacgat tcctcccgtc acgccgataa
       61 cgaggaaggg aaagattccc ggagaaaaag tggtccggag accgtcgaga agcagtgaga
      121 ccatggccgt tcctcctaaa agtagaagag tccctcgggc agagggacca cgaggagttt
      181 atagaatacc ccgtacaccg cgagggagat ggcggctgga aagactgcga gggaaagagg
      241 gttccgcacg ccgaacagca ggagcagagc aaaattgagg gccgctgtgg ccaggatgaa
      301 gccgaaataa tcaagggaca gcacgtacag ggttatgacg gcaagtcctg cgagagctat
      361 gcggatttcc ccggggaaaa ggtcggagag agttcgtccg tctccgtcga atttgcccct
      421 gaaggtgagc ccgccgagaa aaagggcgag ccccccggtc atccacgcga gaagttcggg
      481 gaagaaggcg gctcccacgt agtcgtcctg gatcagggtc tgttcgaaat tggccgccga
      541 atacaggacg aaggctgcca atagaaggaa tgctgcggca atgcagtaat tcgctttttt
      601 cactggtcat tctccttgca gttttggcgg attgaagaga gcggaggggc cgcccggaag
      661 cgagcccctc cctgttccgt aaagatatct gctactgctg cttcttcact tcctcgacga
      721 tgtccgtgag ggcctttacg tcgttgtcga caaaggtctt gaactcttct gacttcatga
      781 accggatgcc cagggtcctg ccgttcataa agccggtgaa ttccttctcc gaggcggctt
      841 tctggaaggc atcggcaaga actgccttca cgtcggcggg ggttcccttc ggaagggcaa
      901 ggcctctcca ggttccggtg acgatggggt acccctgctc cgtcatagtg gggacttcgg
      961 gcagcccggc catgcggaca tcggaggcca cgccgagggg cttgagctgg cccgctttga
     1021 tctgggagat ggcttcgccg ggattgacca tggtgaagtc cacatggccg cccatgaggg
     1081 cgggaatggc ctctgcggca ccattatagg ggatctggtt gaacttcacc ttctggttga
     1141 gctccagggc gagaaggtag aagttgggct tcgcggtgct gccgaatttc acggatcccg
     1201 gagcggcctt cgccgcctcg agaacgtcct tcaccgacgc gaacttcgag tcggggcgga
     1261 cgagcattat ggcgggatcc tcgttgatga gggcgagaag gtcaaaattg tcgggagtga
     1321 tctgggcgag ccccatctgg ggaagccagg cgatctccct ggtgaccatg gtgacggtgt
     1381 atccgtccgc cttggccatg gcgccctcgg tcatgccgac tgctccagaa ccgccggcct
     1441 tgttcatgac caccacgggc tggccgaggt gtttctccgc cgatgccgca agagcgcggg
     1501 ccactgcgtc ggtgccgccg ccggccgcga agggaacgag aaggcggatg ttcttcgccg
     1561 ggaaatcggc cgcaaatgct gcgctgccga agatcagtga agccagagcc agtacgagtg
     1621 ccgctttttt catgtggttc ttcctccttg gatgttggga atgatgatgc cgcgattgat
     1681 gattctaccg ctctcctacc tggaaagcca gccgccgtcc acggcgacga tgtacccggt
     1741 gacgtagtcc gaggcagcgg aagaaagaaa gaccgccgca cccttgaggt cgtcgggagt
     1801 cccccaccgg cccacgggga tcctggcgag gatttccttg ttcctgacag gatccttctg
     1861 gagggcctcc gtattgtccg tggccatgta gcccggagcg atggcgttca cctggatccc
     1921 gaacttcgcc agctcgtttg ccagaagctt cgtcaggcct cccacggcgc tcttgctcgc
     1981 cgtgtaggaa ggaacccgga tccccccctg gaaggaaagc atggacgcga tgttgactat
     2041 ctttccgccc ctgccctgct tttccatctg ccgtgctgcc tcctgggaga ggaagaacag
     2101 gctcttcagg ttgacgttca tcacgtcgtc ccagtccttc tccgaaaatt ccaggatgtc
     2161 cgcccggcgg atgatgcccg cgttgttcac caggatgtcc agcgacccga ggccggccac
     2221 ggtctccgcc acgatgtccg gaatcctctc ctgggaggtg agatcggcct caacgtaaag
     2281 gaactttctg ccgagggacg tcaccgcctt ttccgtctcc ggcattggga tgggacctac
     2341 tcccacgatg tcggcccccg cctcggcaag gccttccgct atgccgaagc cgaggccttt
     2401 ccgcgctccg gtgacgacgg ccaccttgcc gtcaagcctg aacgagtcaa gaatcatctg
     2461 ttttcgcctt cctttcgcgt cttttccgtc aataggttat gaggaccttg aggtactttt
     2521 cgggatcttc cagtatttcc cggaaggctg tgtcgatttt ctcgaaggga tagaccgcgg
     2581 agatcagggg ctcggggttc acctgcccct tttcgaacca ttcgatgacc tggggaaacc
     2641 ggcggcagtt catccgggag ccgacgatct tgagttcctt cttcaccacg atgacctccg
     2701 gaattttggc tggttccggg tgaaagccga gcacgacgat ccgtcccccc ggagcggcat
     2761 atccaaggct ttcctccagc aggcggggca tacccacagc ctccacgacc acgtcggcgc
     2821 cgaattcgcc ggtgaaatcg gccagcgccg cggcgaggtc cccggtcctg ccgttgaccg
     2881 ttttgtcggc cccgagagtt tcggccgcct tcaggcggct gtccgcgaaa tcggttatga
     2941 gcacttccgc ccctgcaagc ttcatggcct gcagaatgca gaggccgatg gttcccgccc
     3001 cgatgacgag gaccttgtct cactcccgca cttcgctcct ggcgagcact tccgccccta
     3061 tggagaaggg ctcgatgagg gcggcttttt cccaggccaa tcctaaggga agggagtaca
     3121 ctttctcctc cggaacggcg atatagtcac agtatcctcc gtctacctgg acacccagac
     3181 acttgacgga actgcagaga ttgtcgtacc ccctgcggca ggaaacacac tttccgcagg
     3241 aaaccacggg atccacggtc acccggtcac cccttgagaa cctggtaacg ccgcttccca
     3301 cggattccac ctcgcccaca agctcgtggc cgagaatcct tggaagggcc acgtccgacc
     3361 gctctccccg gtagatgtga agatcggagc cgcagatccc ggccgccttc acccggacga
     3421 gaacctctcc ttccgtcgga aggggcatcc tgtcctcgaa aatccgtatt tgcttcggag
     3481 caacaagttt ggcacttcgc acgcacttca cctccgctcg ttgcgggcct gcaccgggaa
     3541 acggcagaga cagccggtcc gggcaaaaaa tgatcttcga acaggccgca cagctccggc
     3601 ccaaagtcag cccggaaaaa ccgcgggaat tttgagtcac ttcccctgcc gtcctgtcgc
     3661 agtcttccgg aacaccggcg gggtaaaacc cggagtggcg gtccgcattt tcgggcggga
     3721 gggacccgcg ggtccctccc gccgcttttc gatcctattt gttcgccgct atgatgagat
     3781 caagcagttc cgttccgaac ttcgccttgt actcctccca caccggctgg accgcctgga
     3841 cgaagggagc cacgtccact tcgttgaact tcacgttatg ggtcttcaac tcctccacgg
     3901 tcttgccgac gaaggcgttc cagtattcac gctgtttcgc ctcggcctca tgtcccgcct
     3961 cgaccagggc cttctgctgt tcaggggtga gcttggcaaa agcctgcttg ctgataatga
     4021 ccaggtcggg aaccatgaaa tgccgggtcc atgagaaaat atcggacact tcgtaaagct
     4081 tgagggtcag aagggtgacg gggttgttct cccacccgtc gagcacgccc tgctggaggg
     4141 cgctgtagac ttcgccctgg gccatggggg tggcgatggc gcccatggcg ttaagggtgt
     4201 tcaccatgag cttgctgtcc atgacgcgga tcttgagccc tttcagatcc tcgggcttcg
     4261 tgatggggcg cttgttgttg atgaggttcc gggaaccgcc gtcgccccag tagaggaaga
     4321 cgaacccagc cttctcgatg tcggccttga tcatctcgcc ggccttgccg ttgagggcct
     4381 tgaacatgtg gtccgtgctc tggaagacgt agggaaggga caatacatcc attgtgggga
     4441 caaactgccc
//
LOCUS       DAOTHD010000045         4385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_23677_length_4385_cov_3.046189, whole genome shotgun sequence.
ACCESSION   DAOTHD010000045 DAOTHD010000000
VERSION     DAOTHD010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23677_length_4385_cov_3.046189"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..603
                     /locus_tag="P5219_01415"
     CDS             133..603
                     /locus_tag="P5219_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HRX25444.1"
                     /translation="MKKIRISFPDEKIEALAVLLEDKAPSTCGCLWKALETPVEGLCI
                     HAMWTGREISFPFPGTAFPGDEGLSLPPENQITIPLPGDIVWNAYSPYQWQGNPNIVY
                     DFGVFYGRDSRLLLPMGWKPSTLFARIVENLDPFAAVCARCQGEGRKKLRIERA"
     gene            767..1477
                     /locus_tag="P5219_01420"
     CDS             767..1477
                     /locus_tag="P5219_01420"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRX25445.1"
                     /translation="MAKNAKPMFQSVSDQVFFYLKEKIINNELVPGSVLSIDRLSSEF
                     GISNTPVREAVVRLESLDLVTVNRNRNITVSAMSRDKILDILEMRRLLETYGSRTAAL
                     DIADGEIAALEKIIDAVLADPADFEQYKRSDLELHDAVTRHIKNREIQASLKNLSISS
                     LRIRYYARYYGEENPNLEESVVQITAEHRAILNALKTHDPDRVEQAVAAHLLNAEERT
                     LKALDRFENRRDREAAEK"
     gene            complement(1601..1948)
                     /locus_tag="P5219_01425"
     CDS             complement(1601..1948)
                     /locus_tag="P5219_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25446.1"
                     /translation="MNKKIIVNLVLSIFLYWSAAILLYRDSLFLSSLKNPGEGSLFSG
                     WSLYLLAFSVFALGTLSLLAARAWLRGGADGTGWKQSFARNGILFRFWPVLLLFVLAM
                     IAAFRLAERVPAP"
     gene            2067..3485
                     /locus_tag="P5219_01430"
     CDS             2067..3485
                     /locus_tag="P5219_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HRX25447.1"
                     /translation="MTDLKLVPRFQRFLKNAVFSFLAVLLLTSAAPGAEGRESAKYIF
                     LFIGDGMGEAQVRAAELYSLGSGTGKISFSSMPVRGKMSTSTADGAVTDSAAAGTALA
                     SGFKTKNGILNMDPSGTKRFLTVAMLARDRGMKVGIVTSSFLNDATPAAFYAWRPSRT
                     DWYEIGEQLAESGFDYFAGGGVTRRRGRKGNLRDVYGLAREKGYRVLGTREELAAAKP
                     GAPIIAPGSAGALPYDMDRPKGNLSLAEYTGHGIRLLDNPKGFFLMVEGGTIDWACHR
                     NDAAALVHDMAAFDAAVKEALAFAQTRPKETLIVVLADHETGGMSIDGTASATEVAAV
                     LSNQHRSSEVFDRKIEEFRRGKDRSFEKMLPVIAGSFGLHSLSEEELDKLRAAFTQSM
                     KPRDRRKRDRDYLRRYGPYEPLSVTATRILGRKAGITWSSFGHSGADVPVFAQGAGSA
                     SFAGEYDNTEAGRILMYLLPSR"
     gene            4028..>4385
                     /locus_tag="P5219_01435"
     CDS             4028..>4385
                     /locus_tag="P5219_01435"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HRX25448.1"
                     /translation="MNRSFRNRSGLALFVLLTLAAAAWAAGTGNDWSGAKAKYVFMFI
                     GDGLGLQQVNAAEVFNGSVNRTGGEPAIKKLALSGLPVQGMITTYSTNSFITDSAPAA
                     TSLAAGHKTDNGVIGMD"
BASE COUNT          967 a         1418 c         1241 g          759 t
ORIGIN      
        1 ccgggatcac actgttcatc tgggcgctct tcacctattt cgcccagatc gtactgcccc
       61 ctgctcttct tttctgacga aaagggacgc gggcggcatt cactttgaat tgaaatcagg
      121 gaggataaca ccatgaaaaa aatcaggatc tcatttccgg acgaaaagat agaagccctg
      181 gccgtcctgc tggaagacaa ggctccctcc acctgcgggt gcctgtggaa ggccctggaa
      241 acccccgtgg aagggctgtg catccacgcc atgtggaccg gcagggagat ttccttcccc
      301 ttccccggaa cggcctttcc cggagacgaa gggctctccc ttccgccgga aaaccagatc
      361 accatccccc tgcccgggga catcgtctgg aatgcctact ccccctacca gtggcaggga
      421 aacccgaaca tcgtctacga tttcggcgtc ttctacggcc gggacagcag gctgctcctt
      481 cccatggggt ggaaacccag cactcttttc gcccggatcg tggaaaacct tgaccccttc
      541 gcggcggtct gcgcccgctg ccagggcgaa ggccggaaaa agctcaggat cgaacgggca
      601 taaccggaac gggaccgggg cggcagggga aatcctgccg cctccggatc ccctgcaaag
      661 cgtgcgcatc gggaaaaatt ccgctatgat tgcattgtcc gcggtcattc catcaaaacg
      721 ggatgtcttc ggcacaacct gattgacaaa ggacggtatc acttccgtgg ccaagaacgc
      781 gaaaccaatg tttcagtccg tgtcagacca ggtcttcttc tacctcaagg agaagatcat
      841 caacaacgaa cttgttccgg gcagcgtcct ctccatcgac aggctctcct ccgagttcgg
      901 catcagcaac acgccggtcc gggaagccgt cgtccggctg gaaagccttg acctcgtcac
      961 ggtgaacagg aacaggaaca ttaccgtgag cgccatgagc agggataaaa tactcgacat
     1021 actggagatg aggcggcttc tcgaaaccta cggatccagg acggccgccc tggacatcgc
     1081 cgacggcgag atcgcggccc tggaaaagat cattgatgca gtcctcgcgg accccgccga
     1141 tttcgagcag tacaagcgct cggacctgga gcttcacgac gccgtcacca ggcacataaa
     1201 aaacagggag atacaggcct ccctgaaaaa cctcagcatc tcctccctga gaatccgcta
     1261 ctacgccagg tactacgggg aggaaaaccc aaacctggaa gaatcggtgg tccagatcac
     1321 ggcagaacac cgcgccatcc tgaacgccct gaaaacccac gatcccgaca gggtcgagca
     1381 ggcggtcgca gcccacctcc tgaacgccga ggaacggacc ctcaaagccc tcgacaggtt
     1441 cgaaaacagg cgggacaggg aagcagcgga aaaatagaca atccgtttcc cccatccccg
     1501 agacccgctc cgccgggaaa ctccccccgc ccgtttttta tcctttaccc gccctgcttt
     1561 cagtccctgc ggtcaggccg tttcctgccg ctttgccccg tcatggagca gggaccctct
     1621 cggccagccg gaacgctgcg atcatcgcca ggacgaaaag aagcagaaca ggccagaacc
     1681 ggaaaagaat gccgttcctg gcgaaagact gtttccatcc cgtcccgtct gcccctcccc
     1741 gtaaccatgc cctcgcggca agcagggaga gcgttcccag ggcaaagact gaaaaagcaa
     1801 gcaggtacag ggaccatccc gagaacaggg acccctcccc ggggttcttc agggaggaga
     1861 gaaaaagaga atcccggtac agcagaattg cggcggacca gtacagaaaa atggaaagaa
     1921 ccaggttgac aatgattttc ttgttcatga cggcacctcc gaggacacta tactgacaca
     1981 tccctgcagg aaatgacaag gcaccgaaaa aaaagccgga ttggatataa tggccgcgga
     2041 ggccttcatc ggctttccga gaggagatga ccgacctgaa gcttgtaccc cggttccagc
     2101 gtttcctgaa aaacgccgtg ttttcctttc tggcggtact gctcctcact tcggcggccc
     2161 ccggagccga aggcagggag agcgcgaagt acatcttcct cttcatcggc gacggcatgg
     2221 gggaggccca ggtccgggca gcggagctct acagcctcgg ctccggcacc gggaaaatat
     2281 ccttcagctc catgcccgtc cgggggaaaa tgtccacgtc cacggcggac ggcgccgtga
     2341 cggactctgc tgcggcggga acagccctcg cctccggctt caagacgaaa aacggcatcc
     2401 tcaacatgga tccctccgga acgaaacggt ttctcaccgt ggccatgctc gcccgggaca
     2461 ggggcatgaa ggtgggcatc gtgacctcat ccttcctgaa cgacgccacc cccgccgcct
     2521 tctatgcctg gcgcccctcc cgcaccgact ggtacgaaat aggcgaacag ctcgcggaaa
     2581 gcggcttcga ctacttcgcc ggcggaggcg tcacccggcg caggggaaga aaaggaaacc
     2641 tcagggacgt ttacggcctc gcccgggaga agggataccg ggtgctcggg acccgggagg
     2701 aactggccgc cgcgaagccc ggcgccccga tcatcgcccc cggctcggca ggagccctcc
     2761 cctacgacat ggaccggccg aagggaaacc tctccctggc ggagtacacg gggcacggca
     2821 tcaggctgct ggacaatccc aaggggtttt tcctcatggt ggaaggggga accatcgact
     2881 gggcctgtca tcggaacgac gccgcggccc ttgtccatga catggcggcc ttcgacgccg
     2941 ccgtgaaaga agccctcgcc ttcgcccaaa cacggccgaa agaaacgctc atcgtcgtcc
     3001 tcgcggacca cgagacaggc ggcatgagca tcgacggcac cgcgtccgct actgaggtgg
     3061 ccgccgtact ctcgaaccag cacaggtcct cggaagtttt cgaccggaaa atagaggaat
     3121 tccgcagggg aaaagatcgg agcttcgaga aaatgctgcc ggtgatcgcc ggatccttcg
     3181 ggcttcattc cctgtcggaa gaggaactgg acaaactgcg ggcagccttc acccagagca
     3241 tgaaacccag ggaccggcgg aaaagggaca gggactacct ccgccgctac ggcccctacg
     3301 aacccctttc cgtgacggcc acccgcatcc tcggccggaa ggccggcatc acatggtcgt
     3361 ccttcggcca ctccggagcg gacgtccccg tcttcgccca gggagcgggc tcggcctcct
     3421 tcgccgggga gtacgacaac accgaagccg gccggatact catgtacctt ctgccctcgc
     3481 ggtaacaggc ggcctgtgcc cctgcggacc gcaggccggg tctggtccct gtcatcctgg
     3541 ggccggttct ggttcctgtc atcctgaggg cacgtctctc cgcccgaagg atctcggggt
     3601 aggctttagt tttgtcatcc tcaggccagc tccttgcccg atcgcgtccc cgaaggggga
     3661 ggatctcgct tttgattctg aagaaaccca agtgcgagat ccttcgcatt cgctcaggat
     3721 gacaagctac accctgtcgt cccgaacgag caaagcgata gcgaagcatc cccgcagggg
     3781 gggagggacc tgcttttgat tctgaaggcc ttagcccgag atccctcgca ttcgctcagg
     3841 atgacaatct tgctcaggga gttttcaaat acaaccggga gtccctcccc cgctgcggga
     3901 caaccctctg ccctgatcgc caccctcttt ccatccccaa ctttttacac ccccgataca
     3961 cttccgcgct tcatccgtaa cgctccctcc ggataatggc gtcaaatact tcaaggaggg
     4021 aaaaactatg aatcgatcat ttcggaacag atcaggactg gcgctctttg ttctgctgac
     4081 gctggcagcg gcggcctggg cggccgggac gggcaacgac tggagcggtg cgaaggcaaa
     4141 gtacgttttc atgttcatcg gcgacggcct tgggctgcag caggtcaatg cggcggaggt
     4201 tttcaacggt tccgtcaaca ggacgggcgg cgagcctgct atcaagaagc tggccctgag
     4261 cggcctcccg gtgcagggga tgatcaccac ctactctaca aactccttca tcaccgactc
     4321 ggcccccgcg gccacgtccc tcgccgcggg ccacaagacg gacaacggcg tcatcggcat
     4381 ggacc
//
LOCUS       DAOTHD010000046         4344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_23979_length_4344_cov_3.589182, whole genome shotgun sequence.
ACCESSION   DAOTHD010000046 DAOTHD010000000
VERSION     DAOTHD010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23979_length_4344_cov_3.589182"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /gene="der"
                     /locus_tag="P5219_01440"
     CDS             complement(<1..1129)
                     /gene="der"
                     /locus_tag="P5219_01440"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRX25449.1"
                     /translation="MPVVSIIGRPNVGKSSLFNRLIGRREAIVDDMPGVTRDRLYGEA
                     EWRERKFYVIDTGGFLAKDENAFVQGMRQQVGTAITESDLVLFVIDGKEGPTWMDEDV
                     ADMLRKSGKPVIVVANKIDDGIHEDSVFQAYSLGFEDVIGVSAEHKRYIYDLLDLILE
                     KIPGDEPAPEEDSSVKVAIVGRPNVGKSSILNHLAGEERALVSDIPGTTRDALDTLVK
                     IDGTTFRLIDTAGLRRKSRVEDNIEYYSYVRTLQAIDRCDVALLVMDAEEPFTDQDKK
                     LAGEIIERGKGIVLLLNKWDLLGKKDSLGDSMKKKLREEMVFVSHAPVHFVSALTGRG
                     MQKISDLVLKVFENRKKRIKTTLLNRLLRDILAFDRLPTDNR"
     gene            complement(1151..2542)
                     /locus_tag="P5219_01445"
     CDS             complement(1151..2542)
                     /locus_tag="P5219_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HRX25450.1"
                     /translation="MELLEKGDSFIALFPEQALAASFFALLPPAMQKSALLWPSTGGK
                     KLQDAWIAARKGSVPGIVGGPGAVFAPLPGVRSIIVDEESSGAYRTYRHPFLNMRTVA
                     ARKALLEKASLTLSGRLPSSRVYLRGKPRCTELPPRENVKLVDLKSSFSSEFQGIAGS
                     LPLSAALFSETARVLSMGKVALWLLDRKGYAGEVACEECGKPILCSSCGRVMAWEEQR
                     RRLRCSSCGKVSPLPETCPSCRGVLLSGKRPGLEALLPVARATAPDEKTVLIWDGTKP
                     CGKKAARGLTKDLSGGGIVLGTRSALTVCDLADVGFAAWVDADSEVRSVAFQAKFTAF
                     SMMWESLWRGNSGGGRTVLLQSRRPGFGWQKGMVLGWDHFWNEELRERKELDLPPFSF
                     LLEIKSPSLGLKESIMAQLEKIGLYPMDPGEPPLVFWVTTPSPASVQNVLAPYFSIGN
                     SSTGFPEITVWID"
     gene            complement(2986..4203)
                     /gene="coaBC"
                     /locus_tag="P5219_01450"
     CDS             complement(2986..4203)
                     /gene="coaBC"
                     /locus_tag="P5219_01450"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRX25451.1"
                     /translation="MPDWKQKKKVVLGITGGISAYKTPEIVRALVKAGCDVEVVLTSE
                     GERFVSPMVLSTLAGKRVWRQADFLSDDTGWRIPHIALADWADVILVAPCTAETLSNM
                     ARGAGKELLCSILLAAGAPVVVFPAMNVNMLNHPATARNMEILKETGIFIADPETGSL
                     ACGYEGKGRLPAVEVILEEMWKALCPSKKLEGKNVLVTAGPTWEFLDPVRFLSNPSSG
                     KMGYAMARAAWYRGASVTFVHGPASFSNLSGFGVKPVVSAEEMKRTVLALSEEMDYIV
                     KAAAVGDFRAASFSDRKIKRANADTVTIDLVQNTDIAASLGERKLPGQVLVGFAAESH
                     DLLVNASEKMKRKNLDFIVANDITAADSGFGTDTNTVKLLSRSGTAEEFSGTKEDVAE
                     RVWSSILDEDWLL"
BASE COUNT          913 a         1235 c         1159 g         1037 t
ORIGIN      
        1 ccctgttgtc ggtcggcagc ctgtcgaatg cgaggatatc acggagaagc ctgtttaaca
       61 aagtggtttt aatccgtttc ttcctgtttt cgaaaacttt gagcaccagg tctgatattt
      121 tctgcatccc tcttcccgtc agcgctgaga cgaaatgcac aggggcatgg gaaacgaaga
      181 ccatttcttc cctgagtttc tttttcatcg agtctcccag gctgtccttc ttcccgagga
      241 gatcccattt gttcagaagg agcacaatcc cttttccccg ttcgatgatt tcccccgcaa
      301 gctttttgtc ctggtctgtg aagggttcct ccgcatccat tacgaggagg gcgacatcgc
      361 acctgtcgat ggcctggagc gttctgacgt aagagtaata ttctatgttg tcttcgaccc
      421 tgctttttct tctcagcccc gctgtgtcga tcagccggaa tgtcgttccg tcgatcttca
      481 ccagcgtatc aagggcgtcc cgcgtggttc ccggtatgtc gctcacaaga gccctctctt
      541 cccctgccag gtggttgagg atgcttgatt tgcccacgtt cggcctgccc acgatggcga
      601 ctttcacgga gctgtcttct tccggggcgg gttcgtctcc cgggattttt tcaagaatca
      661 ggtcaagaag gtcgtagatg taccgcttgt gttccgcgct gactccgatc acatcctcaa
      721 acccaaggga ataggcctga aaaacagagt cttcgtggat gccgtcgtca attttgttgg
      781 cgacgacgat gaccggtttt cccgactttc tgagcatgtc ggcgacatcc tcgtccatcc
      841 aggtcgggcc ttcttttccg tcaatgacga aaaggacaag gtcactttcg gtgatggcag
      901 ttccgacctg ctgtctcatc ccctggacaa aggcgttttc atccttggcg agaaagcccc
      961 ccgtatcgat aacgtaaaac tttctttccc tccactcggc ttctccatac agtctgtccc
     1021 tcgtcacccc cggcatgtcg tcgacgatgg cttcccgcct gccgataagc cggttaaaaa
     1081 gcgaggattt gccgacattt ggtcttccta taattgacac tactggcatg ggtgcttttc
     1141 ctccttggcc tcaatctatc cagacggtaa tttcaggaaa accggtgctg gagtttccta
     1201 tggaaaaata aggggcaaga acattctgga cactggccgg cgacggggtt gtcacccaga
     1261 aaacaagagg cggttccccc ggatccatag gatacaggcc gattttctcc aattgtgcca
     1321 ttatactctc tttcagccca agggatggag acttgatttc cagcaaaaag gaaaaaggag
     1381 gcaggtcgag ttcttttctt tcccggagct cttcgttcca gaaatgatcc cagcccagga
     1441 ccataccctt ttgccagccg aaacccggac gcctgctttg cagaagaacg gttcgcccgc
     1501 ccccggaatt tcctctccat aaggattccc acatcatgga aaaggccgtg aatttcgcct
     1561 gaaaagctac gctccgaact tcgctgtccg cgtcgaccca cgcggcgaag cctacgtccg
     1621 ccaagtcgca cactgtcagg gcggaacggg ttccgagcac tatgcctccc ccggaaaggt
     1681 cttttgtgag gcccctggct gcctttttgc cgcaaggttt cgtcccgtcc catatcagaa
     1741 cggttttttc atcgggtgcc gtagcccgtg cgactggaag aagggcttca agccccggac
     1801 gcttccctga gagaaggact cccctgcagg aagggcaggt ctcgggaagg ggcgaaacct
     1861 tgccgcagga agagcagcgg agcctgcggc gctgctcttc ccaggccata acacggccgc
     1921 aggaactgca gagaatgggc ttgccgcatt cttcgcaggc cacctcgcct gcatacccct
     1981 tccggtcaag aagccacagg gcgactttcc ccatggagag aaccctggcg gtttcagaga
     2041 agagagcggc ggaaaggggg agagaaccag cgatgccctg gaattccgaa gaaaaactgc
     2101 ttttcaggtc gacaagcttt acattttccc gtggagggag ttctgtgcac cggggttttc
     2161 cccgaagata cacccttgag gaaggcagtc ttccggaaag tgtcaggctg gctttttcca
     2221 gaagggcctt tctggctgcg accgttctca tattgagaaa gggatgcctg taggttctat
     2281 aggcgccgct gctttcctca tcgacaatta tggaacggac tccggggagg ggagcgaaaa
     2341 ccgcaccggg acctcccaca atgccgggaa cacttccttt gcgggcggcg atccaggcat
     2401 cctggagctt tttccctcca gtggatggcc agaggagagc cgacttttgc atcgcgggtg
     2461 gaaggagggc aaaaaaagag gcagccagcg cctgttcagg aaaaagagca atgaaggaat
     2521 cgcctttctc cagcaattcc acgatttttt tccaccggga gaacgttttg cattcataaa
     2581 ggaaattctc ttcgtattga ccggaagtct gctgattctc cccgtcgagg gaatcaaaat
     2641 caacaagggg gccgggaaaa gagagaatgg aagaaggtac agaaactttc aggacttccc
     2701 cggagccgca aaggaacgtt tttcctgtcc atttgatgag atcgagcgtt ttttctgtca
     2761 gcaacggact gttgtccgaa aggtgcttcc cttggcgaat gttgaaggtt cccttttcag
     2821 gaggggtcgg aaaaaaagaa tccgctattc cgaaccgggt tcccctgcct acggggatct
     2881 tcaatcggct tccccgtgga aggggggatt gcagttcgta ggtcaggggg ttccaccatg
     2941 gtcccggaac aatgacgctg cagtagcagt gagcccccac tctattcaca gaagccagtc
     3001 ctcgtcgagg atgctgctcc acacccgttc agccacatcc tccttcgtcc cggagaattc
     3061 ttcagcggtt ccgcttctgg aaaggagttt caccgtgttt gtgtcggtcc cgaaccccga
     3121 gtcagcggcg gttatgtcgt tggcgacaat aaaatcgaga tttttccgct tcattttttc
     3181 ggaggcgttg acaagcaggt cgtggctttc agcggcgaag ccgacgagaa cctggccggg
     3241 cagcttcctc tctccgaggg aggcagcgat gtccgtgttc tgaacgaggt caatcgtgac
     3301 agtgtcggca ttcgccctct tgatctttct gtcgctgaag gaggcggccc tgaaatctcc
     3361 gaccgcggct gccttgacga tatagtccat ctcttccgaa agggccagga cggtgcgttt
     3421 catctcctcg gcagatacga caggtttgac tccaaatccg ctgaggttag aaaacgaagc
     3481 cggaccgtgg acaaatgtta cggaggcgcc tctgtaccag gccgctctcg ccatggcata
     3541 ccccatcttc cccgaactcg gattgctgag gaaacgtacg gggtcgagaa attcccacgt
     3601 aggtcctgcg gtgacaagca catttttccc ctcgagcttt tttgaagggc acagggcctt
     3661 ccacatctcc tccaggatga cctccactgc ggggagtctt cccttgcctt cgtagccgca
     3721 tgcgaggctt cccgtttccg ggtcggcaat gaaaatcccc gtttccttca gtatctccat
     3781 atttctggcc gtggcgggat gattgagcat attcacgttc atggccggga aaaccactac
     3841 cggggccccg gcagcaagca gaatggaaca gagcagctct ttcccggccc cccgggccat
     3901 gttcgacagg gtttccgcag tgcacggagc gactaaaatt acatcagccc agtccgcaag
     3961 cgcaatgtgg ggtatcctcc atcccgtgtc gtcggaaaga aaatcagcct gcctccagac
     4021 ccgttttccg gccagggtgg acagtaccat cgggctgacg aaccgctctc cttcggaggt
     4081 cagcacgacc tcaacgtcac atccggcctt cacaagagcc cggactatct caggggtctt
     4141 gtaggcggaa atgcctcccg ttatgcccag aacaactttt ttcttctgtt tccagtcagg
     4201 cattggctga ttctgatccg gctccgcttt caggaacgga ttcccgttcc tgggaaaggc
     4261 ggactgcacc gagatcaaat tcctgaaggg cggttgagat gaatttttcg ttgtcctcgt
     4321 cgagtgtacg ccctttctgt tcgc
//
LOCUS       DAOTHD010000047         4322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_24151_length_4322_cov_4.074994, whole genome shotgun sequence.
ACCESSION   DAOTHD010000047 DAOTHD010000000
VERSION     DAOTHD010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24151_length_4322_cov_4.074994"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(354..2594)
                     /locus_tag="P5219_01455"
     CDS             complement(354..2594)
                     /locus_tag="P5219_01455"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX25452.1"
                     /translation="MDRNLEQWLRGDPVQPETVGDSPSEGMADDVAALLGLPVALSRF
                     KSAAGDDAGSSAGKVSDEKTPSGPDTLKPFLTAEPFPPLEPEEIQFGGYFPREDVHGE
                     YEGPEAERGGNASPGSGYDVSGRAGEEPGLAGQEESFFAPVLPVEGQSSAKKGRGRKM
                     AAAGMAAAAVAVGAYFLFGRESTGELLLKGREHSRGGNYAQALVYYNRAAERNPLELD
                     ALLGIAEALEGLDRKGEAVDAYYRCLQVAPGDPRVHGRLGFLLSSMSSYDKAIRSFQE
                     SVNFDPSNAGVFEGMGRAYEAKADYVQAVSAFKKALELDPSSEEYRSALVRVETALSQ
                     QNEEAERLERSILAKEKVLLGRGALALNDLDDSRNNFLQALDLVPDDHDALMGLGDVK
                     KAAGDMEGAAGYYRAVLEFHPDSSLAAAALAEAEKALSAAAQETKPTEGVKKGETPDA
                     KNAGTAPPASTKSPEVKKKPKEGSKPQKTQPSSPVQKKKPAPAVRPRQKAQPQAMIQP
                     PPAARDSLPGFSGRKAEGSSELPKGKKLSGGEKSWKTAKKGSPAADESPETLAGKGVE
                     QFRRGNYSSAFSFFWKRMLLPSSRVSGTAGGDLLVFDGVPFSKGRWREIAPVENGPLE
                     GNIPLSVPLPGSGDGPARWARDKVPLMEAVALNPKEGAVYLNLAMSYILGRKGEGQGI
                     VFGERNEEEQAVYYSLLAHAWLRKGERDKAVLFINAAKRRATGELLGHVLSLESFFLG
                     RERQET"
     gene            complement(2739..2825)
                     /locus_tag="P5219_01460"
     tRNA            complement(2739..2825)
                     /locus_tag="P5219_01460"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2789..2791),aa:Leu,seq:aag)
     gene            complement(2886..3233)
                     /gene="rplS"
                     /locus_tag="P5219_01465"
     CDS             complement(2886..3233)
                     /gene="rplS"
                     /locus_tag="P5219_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRX25453.1"
                     /translation="MDVISLVEKKFLKSDVPDFRSGDTVRVHVKVKEGARERIQVFEG
                     VVIARKHGGLSETFTVRKVSSGVGVERVFPLHCPSISQIEVKRLGKVRRAKLYYLRKR
                     SGKSARIKERREF"
     gene            complement(3282..>4322)
                     /locus_tag="P5219_01470"
     CDS             complement(3282..>4322)
                     /locus_tag="P5219_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRX25454.1"
                     /translation="LEEKKGPAYVVCPSPQGELLSQEIVETLAAREHTVIICGHYEGL
                     DERFIEKRVDREISLGDFVLTGGEIPAMAIIDAMARLVPGVIGKESAVVEDSFFRGML
                     DHPHFTRPSEWEGIPAPDVLLSGHAAEIENWRRSEAVQRTLSRRPELIGRANIRPYLP
                     KGVYIALVHSPVLDRKGKKTTAAVTGLDLSDIGRSCRTYGLDRFLVTTPLASQRELVK
                     TMASHWTSGAGGDVNPDRKEAFSRIKLFPSLARAAEWVTGREKDSPLLIGTTARNREG
                     GFHWIEAKRTVLQKKQPVVFVFGTGAGLHEDVLEHCHIVLQPLSGGFGDYNHLSVRTA
                     VGVVLDRFFGWR"
BASE COUNT          904 a         1290 c         1101 g         1027 t
ORIGIN      
        1 gaggaattcc tccggggtct tcgcccgtcc gaagacactg tacacttcga tgttctctcc
       61 ttcgacgagg ttctgcagaa gattgcctat cctttcggcg aactcctcgt agagagagta
      121 cttttccatg tactttcttc cccattcgtc agttctcgat ccgttcattg cttcatcacc
      181 gcccataata ataaagtgct gcgagcccgc aaaaaccggt ctttagcagg ctatacccct
      241 tgagggaacg atacgttgcc tgagtgacat ttccatgaat acataatcca acggcaagta
      301 tatagtaagt caactccaat gaaaagcccc ggaaggaaat gaaccggaga atgtcaggtt
      361 tcctgcctct ctcttccaag gaaaaaactt tccagcgaga ggacgtggcc aagaagctct
      421 cccgtggcgc gtctcttcgc ggcgttgata aaaaggacag ccttgtccct ctcccccttg
      481 cggagccagg catgggcgag aagagaataa taaacggcct gttcttcctc gttcctctct
      541 ccgaaaacaa tcccctgtcc ttcccctttt cttccgagaa tgtagctcat ggcgaggttc
      601 agatagaccg ctccctcctt cgggttcaga gccaccgctt ccatgagagg gactttgtct
      661 ctcgcccagc gggcaggccc atctccggat cctggaagag gcaccgaaag agggatgttt
      721 ccctccagag gtccgttttc caccggggca atttccctcc accgcccttt gctgaaggga
      781 actccgtcaa acaccaggag gtcgccgccg gcagttccgg aaacccggga ggacggcaga
      841 agcattctct tccaaaaaaa ggaaaaagcg gatgagtaat tgccccgcct gaactgctca
      901 acgcccttcc cggcaagcgt ttctggggat tcatctgctg cgggagaacc tttctttgcg
      961 gttttccagc ttttttctcc ccccgaaagc ttcttgcctt tcgggagttc tgacgaacct
     1021 tccgcttttc gtccactgaa accgggcagg gaatcacgag ccgccggtgg cggctgaatc
     1081 atagcctggg gctgcgcctt ctggcggggt cggactgccg gagcgggttt ttttttctga
     1141 accggggaag aaggctgtgt tttttgtggt tttgagccct ctttcggttt tttcttcact
     1201 tctggagatt ttgttgatgc agggggggcg gtaccggcat tctttgcatc gggggtctcc
     1261 ccctttttca ccccttccgt cggcttcgtt tcctgagctg cagcggagag ggctttttca
     1321 gcttccgcca gcgctgccgc ggccagggag gaatcggggt ggaattcaag gaccgccctg
     1381 taataaccgg ctgccccttc catgtcgccg gctgcttttt taacgtctcc aagccccata
     1441 agggcgtcat ggtcgtcagg aacaagatcc agggcctgaa gaaaattgtt tcggctgtcg
     1501 tccagatcgt tgagagcaag ggctcccctt ccaaggagga ctttttcctt ggcgagaatg
     1561 gaccgttcca gccgttccgc ttcttcattc tgctgcgaaa gggcagtttc cacccgtaca
     1621 agagcggaac gatactcttc cgaagacggg tcaagttcca gggctttttt gaaggcggaa
     1681 accgcctgga cataatcggc ctttgcctcg taagccctgc ccatgccttc aaataccccg
     1741 gcattggaag gatcgaagtt cacgctttcc tgaaaggagc ggatggcttt gtcgtaggag
     1801 gacatggagg acagcaaaaa accgagcctc ccatgaaccc ggggatctcc gggcgcgacc
     1861 tggaggcacc ggtagtaagc atcaacagcc tctcctttcc tgtcgagtcc ttcaagggct
     1921 tcagcaatgc caagaagggc atcgagctca agggggttcc gctccgccgc cctgttgtag
     1981 taaacgaggg cctgggcata gttcccgccc ctggaatgct cacggccttt caggagaagt
     2041 tccccggtgc tctcccttcc aaaaaggaaa taggcaccca cagccactgc agcggcagcc
     2101 attccggcag cggccatttt tctccctctg cctttctttg cggatgactg tccttcgacg
     2161 ggaagcaccg gggcaaaaaa ggattcctcc tggccggcca ggccaggttc ttctccagcc
     2221 cgtcccgaaa cgtcgtatcc ggaccccgga gatgcgttcc cgcccctttc cgcctcaggt
     2281 ccttcgtatt ctccatgtac atcttcccgc gggaaatatc cgccgaattg gatttcctcc
     2341 ggttcgagag gggggaatgg ttcagcggta agaaacggtt tcagggtatc aggccctgat
     2401 ggagtctttt catccgatac ctttccggcg gaagaaccgg catcatcccc ggccgcactt
     2461 ttgaagcgtg aaagggctac gggaagacca agaagggcgg caacgtcatc tgccattcct
     2521 tcagaaggcg aatctcctac ggtttcaggc tgaaccggat ctcccctcag ccattgttcc
     2581 aggtttctgt ccattgcccc accccttggt aaaccggaat ccctcaaagt gtactaaaaa
     2641 aaagacgtcc gggccagtcc cggagagaaa gaattctctt ccggcagaat gcgaaaaaaa
     2701 aagcttttct cttctcagag aaaagctttc gtttctgatg gtgcggaggg ggagactcga
     2761 actcccacag ccttgcggcc acaaggtcct tagccttgcg cgtctacctg ttccgccacc
     2821 cccgcgcaac gacggatatt ataatcgccc ttcccgagag agtcaagaga ccggggaaaa
     2881 gaaaactaaa attctcttct ttccttgatc ctggcggatt ttccgcttct cttccggagg
     2941 tagtacagct ttgcccggcg aaccttgccc agtctcttca cctcgatctg gctgatggaa
     3001 gggcaatgca ggggaaagac gcgctccacg ccgacgccgc tcgacacttt tcttacggtg
     3061 aaggtctcgc tgagcccgcc gtgttttctg gcaatgacaa cgccttcaaa cacctgaatt
     3121 cgctctctcg ctccttcttt caccttcacg tgcacccgaa cggtatcgcc cgaacggaaa
     3181 tcaggaacat cgctcttcaa aaatttcttt tccacaagac tgattacgtc cattgtaaaa
     3241 ccctccttgc gtccatttca ctattctctg ttcaaatttt gtcaacgcca tccgaaaaaa
     3301 cggtccagca cgacgcccac ggctgtcctg actgaaagat ggttgtaatc gccgaatcct
     3361 ccggataggg gctgcagaac tatgtgacag tgttcaagca catcttcatg gagtcccgcc
     3421 cctgtaccga agacaaatac caccggctgt ttcttctgaa gcacggttct tttcgcctca
     3481 atccagtgaa atccgccttc cctgtttctc gccgttgtcc cgatcagcag cgggctgtct
     3541 ttctccctgc cggtcaccca ttccgccgct cttgcaaggg aagggaacag ctttatcctg
     3601 ctgaatgctt ctttcctgtc aggattgacg tcacctccgg caccgctggt ccagtgggaa
     3661 gccatggttt taaccagttc tctctggctt gccagcggcg ttgtcacaag aaacctgtca
     3721 aggccgtagg tcctgcagga acggccgatg tcggaaaggt cgagcccggt gaccgcagcc
     3781 gtcgtttttt tgcctttcct gtcaagaacc ggagaatgca ccagggcgat ataaaccccc
     3841 ttgggcagat agggacgaat attggccctc cctatcagtt caggtcttct tgacagtgtt
     3901 ctttgcacgg cttcactcct gcgccagttc tctatttccg ccgcatggcc cgaaaggaga
     3961 acatccggag cgggaattcc ctcccattcc gagggacggg tgaagtgagg atgatcaagc
     4021 atcccgcgga aaaatgagtc ctccaccacc gccgactcct tcccgatgac cccgggaaca
     4081 agacgggcca tggcatcgat gatggccatg gcggggattt ctccgccggt gaggacaaaa
     4141 tcgcccaggg agatttcccg gtccaccctt ttttcgataa accgctcatc gagcccttcg
     4201 tagtggccgc aaataatcac cgtgtgctcc ctggcggcaa gggtctccac tatttcctgg
     4261 gacaggagtt cgccctgggg cgacggacag accacgtagg ccggtccttt cttctcctca
     4321 ag
//
LOCUS       DAOTHD010000048         4307 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_24268_length_4307_cov_3.213076, whole genome shotgun sequence.
ACCESSION   DAOTHD010000048 DAOTHD010000000
VERSION     DAOTHD010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24268_length_4307_cov_3.213076"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /gene="nrdD"
                     /locus_tag="P5219_01475"
     CDS             complement(<1..562)
                     /gene="nrdD"
                     /locus_tag="P5219_01475"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HRX25455.1"
                     /translation="MAKKADEQATFLTRGEGSAWGGEKEVPGRQMVMFSPGGESTDLA
                     LMVDALAHEETQPWHPSRIRDALVKEAGVEPGLAGEIASEVESDLLRWGRDRVTTSLV
                     RELVNVKLFQRGLDAKLQDHSRIGIPVHDLETMMLNPNRENSNTTHNPESINLSIAEL
                     ALKQYALTKVFSRDVAEAHLSGTIHLH"
     gene            complement(546..1031)
                     /gene="nrdR"
                     /locus_tag="P5219_01480"
     CDS             complement(546..1031)
                     /gene="nrdR"
                     /locus_tag="P5219_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HRX25456.1"
                     /translation="MRCPSCGAMETRVIETRTADEGRIVRRRRECPECSNRFTTYEKA
                     EEKRTLRVIKKDGSRETFDRDKIIRGIGRACEKLPVSLEQIEDLASKIEDDLKGEGFG
                     EVPVSEIGRRVMDGLRKVNQVAYVRFASVYREFTDLQSFQHEIARLIGEKEVSRNGEE
                     S"
     gene            complement(1257..1721)
                     /locus_tag="P5219_01485"
     CDS             complement(1257..1721)
                     /locus_tag="P5219_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRX25457.1"
                     /translation="MIDLEKRISGDLLAAMKEKKEMELSSLRMLKAEFQRAKTEKGHA
                     GELTEDEVVSLVQRLVKQRREAADQYTAVGAGERAAQELKEALFLETYLPEQLSDEEL
                     GQIVEEAGAFAKAAGPKDMGRVMGRVMTAVKGRADGKRVKARVQEYLQSLEE"
     gene            complement(1728..1916)
                     /gene="rpsU"
                     /locus_tag="P5219_01490"
     CDS             complement(1728..1916)
                     /gene="rpsU"
                     /locus_tag="P5219_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRX25458.1"
                     /translation="MTTVVRRENESLDDALRRFKREVSKVGTLREVRKREHYEKPSEA
                     KKTKRAEAARKRRTKSRG"
     gene            complement(1949..2290)
                     /locus_tag="P5219_01495"
     CDS             complement(1949..2290)
                     /locus_tag="P5219_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HRX25459.1"
                     /translation="MDGKCLFCEISAGRIPADIVYGDDDVFVIRDIAPQAPVHLLVIP
                     REHVESAAHVRKPEVWSKVMDCAVQTACRMGIDPGGYRLVVNCGAQAGQTIPHLHIHL
                     LAGRSFRWPPG"
     gene            complement(2318..3325)
                     /locus_tag="P5219_01500"
     CDS             complement(2318..3325)
                     /locus_tag="P5219_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRX25460.1"
                     /translation="MAVPLELLDCFEILGVSPGAHPGEIRSAFRRLALSFHPDVAGPQ
                     GACRFEAIAAAYSVLKSASPVEIADALKKGRKAPGTAPGKDRGGSPFRWRRKKQAPPS
                     PSEKKKEEEDGSGRVRELLLERALVEAELSVARLLEKAGEGENDGDVSRVVRRLLGAH
                     PEVRLLALRSLGRKAAEGEVFAALLEMVRLWPVDEAVLERLLLPDYSPECRRKMAEAL
                     IARFSSLPEGPALAFLRWISPLPGREGFLGKALSHQSPRVLAAALSLWTGGGLPDDLA
                     LLRLLKRDEDCVLIPLLRVLKVRGVPPWAVPRLTALSEKHPSPAVRVWARSIVRAENL
                     V"
     gene            complement(3327..>4307)
                     /locus_tag="P5219_01505"
     CDS             complement(3327..>4307)
                     /locus_tag="P5219_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRX25461.1"
                     /translation="RGTPSCGGRDFDAALALLLAEKAGANPGGVDSPLHRLLLAEAEE
                     VKIALSGCLNHEWTPPAGVGASSVAVTRNEFENLVRPSLERVVSMAEDLWKFHGPSRL
                     LLVGGGSRIPLLRTLLAGRMARPERLSLCPDEAVVIGAALYGSQARSERLLLDVLSDS
                     LGILAADGTPVPLLEKGMHLPARVEKKFESVGSGPFTLRVFQGDGGRIISTVRIPDAK
                     KGESVVLVFAVDSGGLLRIDILREGGGTTVIPPLDMGTPDAPFFASPPEELLELEKRF
                     ARVSPVLSQAQQARGEALFRTVRGLRDEECYPGGVESLEQMVAEMERVVR"
BASE COUNT          789 a         1422 c         1247 g          849 t
ORIGIN      
        1 cgtggagatg gatggtcccc gacaggtggg cttccgccac gtccctggag aataccttgg
       61 tgagggcgta ctgcttcagc gccagctcgg cgatggacag gttgatggat tccgggttgt
      121 gggttgtgtt gctgttctcc cggttggggt tcagcatcat ggtctccagg tcgtggaccg
      181 gaatgccgat ccggctgtgg tcctggagct tggcgtccag gccccgctga aagagcttga
      241 cgttgacgag ctccctgacc agagaggtgg tgaccctgtc cctaccccac cgcagcaggt
      301 cggactccac ctcggaggcg atctccccgg caaggcccgg ctccacaccg gcttctttca
      361 ccagggcgtc ccggatcctg gacgggtgcc agggctgagt ctcctcgtgg gcgagggcgt
      421 ccaccataag ggcgaggtcg gtggactctc cccccgggga gaacatgacc atctgccgcc
      481 cgggcacctc cttttcaccg ccccatgccg aaccttcccc ccttgtgagg aaagtcgcct
      541 gttcgtcagc tttcttcgcc atttctggaa acctcctttt caccgatgag gcgggctatt
      601 tcatgctgga agctctgaag atcggtgaac tccctgtaga ccgaggcgaa gcgcacgtag
      661 gccacctggt tgaccttccg gagaccgtcc atgaccctcc ggccgatctc cgacaccgga
      721 acctccccga agccttcacc cttgagatcg tcctcgatct tcgaggccag gtcctctatc
      781 tgctcgaggg agacagggag cttctcgcag gccctgccga tgccccgtat gatcttgtcc
      841 cggtcgaaag tttccctgct cccgtccttc ttgatcaccc ggagagtccg cttttcttcg
      901 gccttctcgt aggtggtgaa gcggttcgag cattcggggc actcgcggcg gcgccggacg
      961 atccggccct cgtcggcggt ccgtgtctcg atgacccggg tttccatggc tccgcaggat
     1021 ggacatctca tgggcgaacc tccctatata tagtacaacc ttgggtatga taccccgata
     1081 tatggtgctt ttccagagag acgaaattcc ctattctccg ggacccggtg cctatagccg
     1141 gttgtccaca atgtcacccg ggaaatgtca aagaaaaggg atgaaaaaaa gccgggtttc
     1201 cccggctttc tgtttactgc ttatttttcg gattgcccgc aacgctggga aaaaggctac
     1261 tcctcgaggg actggagata ctcctgaacc ctggccttta ccctcttccc gtcggcacgg
     1321 cccttgactg ccgtcatgac cctgcccatg acccggccca tgtccttggg accggcggct
     1381 ttcgcgaagg cgcccgcctc ctcgacgatc tgcccgagtt cttcgtcgct gagctgctcc
     1441 ggaaggtacg tctcgaggaa gagggcctct ttcagttcct gggcggcacg ttcgcccgcc
     1501 ccgacggccg tgtactggtc cgccgcctcg cggcgctgtt ttacaagacg ctgcaccagg
     1561 gaaacaacct cgtcctcagt cagctcgccc gcgtgcccct tctccgtttt ggccctctgg
     1621 aattccgcct tgagcatccg gagggaggaa agctccatct ccttcttctc tttcatggcg
     1681 gccagaagat caccggaaat ccgtttctcg agatcgatca cagtgctcta ccccctggac
     1741 ttggtccgcc tcttccgcgc ggcctcggcc cgcttcgtct tctttgcttc actgggcttc
     1801 tcgtagtgct ctctctttcg cacctcgcga agggttccaa ccttggagac ctcgcgcttg
     1861 aaccgtctga gagcgtcatc aagggattcg ttttctcttc gaacgacagt cgtcatacac
     1921 cattcccccc tttcttcgcc acccgcatct accccggtgg ccagcggaaa ctgcgtcccg
     1981 ccaacaggtg aatatgaagg tggggaatcg tctgcccggc ctgcgcccca cagttgacta
     2041 ccagcctgta tccgccggga tcaatcccca tgcggcaggc cgtctgcacg gcacagtcca
     2101 tgactttcga ccacacttcg ggtttccgga catgggcggc tgattccacg tgttctctcg
     2161 gtatcacaag aagatgcacg ggcgcctgcg gggctatatc ccgaatgacg aacacatcgt
     2221 cgtcaccgta gacaatatcc gcagggatcc ttcctgccga aatttcgcag aacagacact
     2281 ttccgtccaa aaatcccacc tcacagctca cagtattcta taccagattt tcggcccgca
     2341 caatactccg ggcccaaact ctgacagccg gggaaggatg tttttccgat agtgcagtca
     2401 gcctcggaac cgcccatgga ggaactcccc gcactttcag gaccctgagc agcggaatga
     2461 gaacgcagtc ctcatcccgc ttgagcagcc ggagaagggc caggtcatcg ggaaggcctc
     2521 ctccggtcca gagggaaagg gccgcggcga gaacccgggg agactggtgg gaaagtgctt
     2581 tgcccaaaaa accctccctc cccggaaggg gagagatcca ccggagaaaa gccagagccg
     2641 gcccttcagg cagggaggaa aacctggcaa tcagggcttc cgccatcttc ctcctgcact
     2701 ccggagaata gtccgggagg agaagccgtt caagaaccgc ctcatccacg ggccagaggc
     2761 gcaccatctc gagaagggcg gcgaagacct ccccttccgc ggccttccgc cccagggacc
     2821 tcagggcaag aaggcggact tcggggtggg cgccgagcag gcggcgcacg acccgggaaa
     2881 catccccgtc attttcccct tctcccgcct tttcaaggag cctggccacc gaaagctccg
     2941 cctcgacaag agcccgctca aggagcagtt cccggacccg ccccgagccg tcttcctctt
     3001 ccttcttctt ttccgacgga gacggaggag cctgtttttt tcttctccag cggaagggag
     3061 aaccgccccg atccttcccc ggcgcagtgc cgggggcttt tcttcccttc ttcagggcgt
     3121 cggctatctc tacgggactc gccgacttca ggacggaata ggctgcggca atggcctcga
     3181 accggcacgc tccctggggt ccggccacat cggggtggaa ggagagggca aggcggcgga
     3241 aggcggagcg gatttcgccg ggatgggctc ccggagaaac gcccaggatt tcgaagcagt
     3301 ccagcaattc caggggaacg gccatatcag cgaaccaccc tctccatttc ggccaccatc
     3361 tgctccaggg actccactcc cccggggtag cattcctcgt ccctgagccc cctgacagtg
     3421 cggaagagtg cttccccccg ggcctgctgc gcctgagaca ggaccgggga tacccgtgcg
     3481 aagcgttttt cgagttcgag gagttcttcc ggcggagagg cgaagaaggg ggcgtccgga
     3541 gtccccatgt cgaggggggg aatgaccgtc gtcccgccgc cctccctcag gatgtcgatc
     3601 cggagcagcc cccccgaatc gaccgcaaaa acaaggacga cagactcgcc ctttttcgca
     3661 tcaggaatcc tcacggtgga aatgatccgc cccccgtctc cctggaagac ccgaagggtg
     3721 aagggaccgc tcccgacgct ctcaaatttc ttttccaccc tggcaggaag atgcatgccc
     3781 ttctccagca gcggaacggg agttccatct gctgcgagaa tgccgaggct gtcggaaagc
     3841 acgtccagga gcagccgttc cgacctggcc tggctgccgt agagagcggc gcctatgacc
     3901 accgcctcgt cggggcacag gctgaggcgt tcaggcctgg ccatcctccc ggcgagaagg
     3961 gttctgagaa ggggaatgcg gcttcctccg ccgacgagaa ggagcctgga aggcccgtgg
     4021 aatttccaga ggtcttccgc catggacaca acccgctcca gggagggccg gaccagattt
     4081 tcaaactcgt tccgcgtgac cgccaccgaa gatgcaccca ccccggcggg aggggtccac
     4141 tcatggttga ggcagccgga gagggcaatc ttgacctcct cggcttcggc cagcagcagc
     4201 cggtgaaggg gagagtccac tcctccgggg ttcgcgccgg ccttttctgc gagaagcagc
     4261 gccagggcgg catcgaaatc ccggccgccg caggagggcg ttcccct
//
LOCUS       DAOTHD010000049         4282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_24455_length_4282_cov_3.431512, whole genome shotgun sequence.
ACCESSION   DAOTHD010000049 DAOTHD010000000
VERSION     DAOTHD010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24455_length_4282_cov_3.431512"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="P5219_01510"
     CDS             <1..689
                     /locus_tag="P5219_01510"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRX25462.1"
                     /translation="IGTLYTPHLTAHMSAKRAKYTLDDFAMLFNLVTDPGVLVVPASS
                     PFNSIQDIIDAEKAAPGTLTGSTSGPGGDDAFALAQFNEATGCTVKSVPSTGSSNAKA
                     TVMGGHVSMGFMNLSQIESNLKAGEVRVLAMMTAKRHPNVPEIPTFAELGYKVISDSS
                     RGFAAPAGIPEEALKKIIETFEKVLADAEFQAAAKEQLQMNVMTPEEYKAYLEDLLVV
                     TNKAYEKDPW"
     gene            704..1144
                     /locus_tag="P5219_01515"
     CDS             704..1144
                     /locus_tag="P5219_01515"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRX25463.1"
                     /translation="MTRSSMNRLFAAGGLGLSAVLFLGANSFPDRAAAAARYIFFLAG
                     MLAVLSLILLLQKTSSPDSHVRWVRSPKNFTLTLASMVGYGILISFIGFFPASALFMA
                     ALSWMLGFRRPVFILLGTGLILGFVWLVFVHFLGVPVPMGIWGE"
     gene            1148..2632
                     /locus_tag="P5219_01520"
     CDS             1148..2632
                     /locus_tag="P5219_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRX25464.1"
                     /translation="MTEFFLPALSELMNPGVLFAILSGTVVGIIVGAMPGLTATMAVA
                     LLIPVTFGMQPLVGLALMGGVYSGGMYGGAISSILLSTPGTPAAAATAFDGYPMTKQG
                     KGGTALTVATWASFWGGIFSTVALLLMAPALAKFSLRFGPPEYFVLAIMGLSSIVTLT
                     KGSMVKGLISGFLGLVLATVGMDPISGFMRFTFDIVDLYDGIPFMPALIGLFSVSQIL
                     DLTAETHIVEDLSDTIASIKRSRLPRGLGPTIAKGSIIGTIVGMLPGAGATISAFISY
                     NFAKQSSRDSDTFGKGNPKGVAASESANNGCVGGSLIPLLTLGIPGNSVAAALMGGLL
                     IQGLIPGPELFSKYGAMTYGFILSLFIANIIFLVLGIYLAPYFAKVTMTPNALLIPGI
                     AILSVIGSYAINNNMFDVWLMIGFGAVGYFLEKGGFSTGALVLGLILGPIAELGFGQS
                     LIMAAGSPMIFFERPLCMLLWAITILLMVPAFSGHLKKSKNKVD"
     gene            2957..>4282
                     /gene="mgtE"
                     /locus_tag="P5219_01525"
     CDS             2957..>4282
                     /gene="mgtE"
                     /locus_tag="P5219_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRX25465.1"
                     /translation="MKDIQNKLLASVEKLFERKQFKSLKELLSTMEPADIAEVLAEKE
                     PAERVFLFRLLPKDIAIEVFEFLEGTEREELLGSFTDSEVAAIIEEMSDDDRTALFDE
                     LPAKTVKKLLAHLSPEERKLANALLNYQPDSAGRIMTPEFIDLKETMTASQALARIRA
                     TAAKKETIYTSFVVSPERKLKGTVHLEDLILASPDTPVTEFMDGLPVFVTTDTDQEEA
                     ARTMSKYDLQTVPVVDREHRLVGILTFDDILDIVQEEATEDFERMAGISPVDESYLDA
                     GILTLTRKRLTWLLVCIVTQLFSSFILEHYSFALESVVALAFFIPLLTGTGGNTGTQA
                     ATLVIRGFTVGEIVREDLFRVISKEVISGLILGVSLAALASIRAWTMGTGYGVALTVA
                     ASVVGVVMLGNLVGAFLPFAAKKLNIDPAVMSGPFITTVVDILGLLLYFE"
BASE COUNT          824 a         1375 c         1154 g          929 t
ORIGIN      
        1 ccatcggaac cctgtacacg ccccatctga cggcccacat gtccgccaag cgggcaaagt
       61 ataccctcga tgactttgcc atgctgttca accttgtcac cgatcccggt gtcctggttg
      121 tccccgcctc cagccccttc aattccatcc aggacatcat cgacgcagaa aaagcggctc
      181 ccggaaccct caccggctcc acgtcaggcc ccggcggaga cgacgctttc gccctcgccc
      241 agttcaacga ggccacgggc tgcaccgtga agagcgtccc ctccacggga tcctccaacg
      301 ccaaggccac ggttatgggc ggccacgtgt ccatgggttt catgaacctt tcccagatcg
      361 agtccaacct caaggccggc gaggtcaggg tcctggccat gatgacggca aaaaggcacc
      421 cgaacgtgcc cgaaatcccc accttcgctg aactgggata caaggtcatc tccgactcgt
      481 ccagggggtt tgccgcacct gcgggaattc ccgaggaagc tttgaagaaa atcattgaga
      541 cctttgaaaa agtgcttgca gatgccgaat tccaggcggc cgccaaggaa cagctccaga
      601 tgaacgtcat gactcctgaa gagtacaagg cgtacctcga ggatctcctt gtcgtaacca
      661 acaaggccta cgagaaagat ccctggtagg gcgaggctct ttcatgaccc gatcttccat
      721 gaaccgtctt ttcgcggccg ggggattggg actttccgcc gtcctgtttc ttggggcaaa
      781 cagctttccc gaccgggccg cggcagcggc ccggtacatc tttttcctgg cgggaatgct
      841 cgcggtcctg tccctgattc tcctgctgca gaagacatcc tcgcccgatt cccatgtccg
      901 gtgggtccgc tctccgaaaa acttcactct gaccctggca tccatggtcg gctacggaat
      961 tcttatttcc ttcatcggat ttttcccggc cagcgccctt tttatggccg cactctcctg
     1021 gatgctcggc ttccggcgcc ccgttttcat tcttctcggt acaggcctga tcctcggctt
     1081 cgtctggctc gtgtttgttc atttccttgg ggttcccgtc cccatgggca tctgggggga
     1141 atagaccatg actgaatttt ttctgccagc gctttcggaa ctgatgaatc caggcgttct
     1201 cttcgccatt ctttcgggca ctgtggtggg catcatcgta ggcgccatgc cgggcctcac
     1261 cgccaccatg gccgtggcgc tgctcatccc ggtgaccttc ggaatgcagc cccttgttgg
     1321 cctggccctt atgggcggag tctacagcgg cggaatgtac ggaggggcca tctcatcaat
     1381 cctgctctcc actccgggaa cccccgccgc agcagccacg gcattcgacg gctaccccat
     1441 gacgaaacag ggcaagggcg gcaccgctct caccgtggcc acctgggcca gcttctgggg
     1501 aggcattttc tccaccgtgg ccctgctcct catggctccc gccctggcaa agttctccct
     1561 ccggttcggc ccaccggaat atttcgtcct ggccatcatg ggcctttcca gcattgtcac
     1621 cctcacgaag ggaagcatgg tgaaggggct gatctccgga tttctcggcc tggtactggc
     1681 cactgtgggc atggatccca tctcgggctt catgcgcttc acctttgaca tcgttgacct
     1741 ttatgacggc attcccttca tgcccgctct catcggcctc ttctccgtga gccagatcct
     1801 ggacctgact gcggagaccc acatcgttga ggatctgagc gacactatcg cctccatcaa
     1861 aaggagcaga cttcccaggg gactcggccc cactatcgca aaggggagca tcatcgggac
     1921 aatagtcggc atgctgcccg gagccggtgc gaccatatca gctttcattt cctataattt
     1981 cgcgaagcag agctccaggg attcagacac cttcggaaag gggaacccca agggagtggc
     2041 agcctccgaa agcgccaaca acggctgtgt gggaggttcc ctcatccccc tgctcaccct
     2101 cggcattccc ggcaacagcg ttgcggcggc cctcatggga ggtctcctga tccagggtct
     2161 cattcccgga ccggagcttt tctcgaagta tggggccatg acgtacgggt tcattctctc
     2221 ccttttcatt gcgaatatca ttttcctggt actgggaatc tacctggcgc cctacttcgc
     2281 caaagtcacg atgaccccca acgcgctcct cattccgggc attgccattc tttcggtcat
     2341 cggaagctac gccatcaaca acaacatgtt cgacgtgtgg cttatgatcg gcttcggcgc
     2401 cgtcggatat ttcctggaaa agggcggatt ctcgaccgga gccctcgtcc tggggctcat
     2461 cctcggcccc attgcggagc tgggcttcgg ccagtccctc atcatggccg caggctcccc
     2521 catgattttc ttcgagcggc ccctctgcat gcttctctgg gccatcacca ttcttctcat
     2581 ggttccggct ttttcgggac acctgaagaa gagcaaaaac aaggtggact gacaggaggg
     2641 ccggctcttt tgagccggtc ctccttttct ccctccggaa gatccgctgc ctcgacattt
     2701 cccccccttt atgatacttt tttcccgttg ttcccaattc caccggctca aggagcgttt
     2761 tcaatggaca gcggaccaca acaaaaagcg gaatcaatga tgccgtaaca gcgccgcccg
     2821 ccctcccttt cggcggaatt ccggcgcgtg ccgggactgt ttctccgccg gacatcccga
     2881 aaaaacatgt gaaaaggcac ggaagacgtt ccgccgggag cgtctccgtt caggccatgg
     2941 gacaggggga agggtcatga aggacatcca gaacaagctt ctcgctagcg ttgaaaaact
     3001 cttcgaacgg aagcagttca agagtctcaa ggaactgctg agcaccatgg aacccgcaga
     3061 catcgccgaa gttctggcgg agaaggaacc tgcggagcgg gtcttcctct tccgcctcct
     3121 tcccaaggat atcgccatcg aagttttcga gtttctcgaa ggaaccgaac gggaggagct
     3181 tctcgggagc ttcaccgatt cggaagtggc ggccatcatc gaggagatgt ccgacgacga
     3241 ccggacggct cttttcgacg agcttcccgc caagacggta aaaaagctcc tggcccatct
     3301 ttccccggaa gaaagaaagc ttgccaacgc ccttctgaac taccagccgg attccgcggg
     3361 gcgcatcatg acccccgagt tcatcgacct gaaggagacc atgacggcct cccaggccct
     3421 ggcgagaatc agggcaacgg cggcgaagaa ggagaccatc tatacctcct tcgtggtcag
     3481 ccctgagaga aagctcaagg ggacggtgca cctggaggac ctgattctcg cctcccccga
     3541 tacccccgtc acggaattca tggacggcct gccggtcttc gtcacgacgg acaccgacca
     3601 ggaagaagcg gcgaggacca tgtcgaaata cgacctccag accgttcccg tggtggacag
     3661 ggagcaccgc ctcgtgggaa tcctcacctt cgacgacatc ctggacatcg tccaggaaga
     3721 ggccaccgag gacttcgaac ggatggccgg tatctccccg gtggacgaaa gctatctgga
     3781 cgccgggatt ctgaccctca cccgaaagag gctgacctgg ctgctcgtct gcattgtcac
     3841 ccagcttttc tcgtccttca tccttgaaca ctattccttc gcccttgaga gcgtggtggc
     3901 cctcgctttc ttcatacccc ttctcacggg caccggcggc aacaccggca cccaggcggc
     3961 tacgctggtg atccgcggct ttacggtcgg tgaaatcgtc agggaggacc ttttcagagt
     4021 catctcgaag gaggtcatct ccggcctgat cctgggggtt tctctcgcgg cgctggcatc
     4081 catccgggcg tggaccatgg ggacagggta cggtgtggcc ctcaccgttg cggcatcagt
     4141 ggtgggggtg gtcatgctgg gaaatctcgt gggagccttt cttcccttcg cggcgaaaaa
     4201 gctgaacatc gacccggcgg tcatgtccgg ccccttcatc accacggtgg ttgacatcct
     4261 gggccttctg ctttacttcg aa
//
LOCUS       DAOTHD010000050         4281 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_24462_length_4281_cov_2.684572, whole genome shotgun sequence.
ACCESSION   DAOTHD010000050 DAOTHD010000000
VERSION     DAOTHD010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24462_length_4281_cov_2.684572"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="P5219_01530"
     CDS             complement(<1..276)
                     /locus_tag="P5219_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HRX25466.1"
                     /translation="MWQFFTERGKKVVQLAHREALRLGHDVIGTEHILLGILAEGEGA
                     AVQSMESLGINLHEMRLRIEESVGKSHPILKPVDLPLSPRAKRVLDLS"
     gene            complement(324..803)
                     /locus_tag="P5219_01535"
     CDS             complement(324..803)
                     /locus_tag="P5219_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HRX25467.1"
                     /translation="MASLTRVIENYINNLFSEMDEQAVSLRRKELAEIFGCVPSQINY
                     VLRSRFTPERGYIVESQRGGHGYIRIFRLCYDIPEERINHIEEIVGDAVTDQEAKRLL
                     TSLQERGMIDSRERILIEVALRHADELGSSEFDVSPYKRNAIQAELLKRMLRSLALA"
     gene            complement(910..1509)
                     /gene="plsY"
                     /locus_tag="P5219_01540"
     CDS             complement(910..1509)
                     /gene="plsY"
                     /locus_tag="P5219_01540"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRX25468.1"
                     /translation="MSFLFWILLGYLAGSFPTGYLAARAFRGVDIRTFGSGNVGATNV
                     GRLMGKKWAVAVTLLDMIKASVGILCARAAGVESSWTIAFTGFAGVCGHNFPLWLGFR
                     GGKGVASTYGVVFFLHPPLSFFLAPAGGVIWFALLKLKGYVSLASLISLWCLPGIAAA
                     LSFPAPFLALLTALAALSTTRHRDNIRRLFEGKESSFKK"
     gene            complement(1506..3278)
                     /locus_tag="P5219_01545"
     CDS             complement(1506..3278)
                     /locus_tag="P5219_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRX25469.1"
                     /translation="MKEKYRLPGMGKTLLAGKGEDSFPLTLAGLEPLSSLEENALPQT
                     VLIGENDGPRGKRILEECVSHGIPFVVLGRGWPRDVPAAVRNLAKKTGSKIIGPGSGG
                     LVTPFLSGDGRSKTALLAEGRDLFQRTVCESHERSVPMRYAFSFGEKSDLSPVEAARA
                     ILELDGEVRFFVFLLERLRKGRDLLEFAAEAAGRGKCVGLLEYDPLSGEDRVRESALR
                     QFGIVSLHEPSQVVDGAAAFLACGGERHRGILLEAGQGEMARLVRSEASRRGIPLLDQ
                     DDGSSPVLKVLRNCVHTSPRRPMAEKTGDKKKPCLLASSLSFPREENGRGPEDPKTPL
                     IPGIGRSLDALSYTFFPEAATMPGGTEKPILPGCPIHPRPTEYDAKVLLGAFGIPVAR
                     ERLCRSFREAAEAAEEIGYPVALKVMSPNILRKSEARVVALNLQDREELRNAYGRTLE
                     KARVANPRARIRGVLVQEMIRGGTEWRMEFRRDSRFGPLVETGISGVYTEILPDGVIR
                     VAPFDEKEALCMIRESRGYPLLLEGWRRDPLDMVAFASALSAFSRLAFCEREVARLDV
                     NPLFVNKKGVLVVDAFIEKKGRKK"
     gene            complement(3371..>4281)
                     /locus_tag="P5219_01550"
     CDS             complement(3371..>4281)
                     /locus_tag="P5219_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25470.1"
                     /translation="FLSTAGTADASVLAGMSDLCRVKILVNFPDDVAKVEDIVQKEFL
                     ADLSRSVPLSSLDSPDRYGYPAVSYVLSLNRERGALREWAKYKDLPFTLEIRTVLQEV
                     WAANAPKVSLPTDTSTKKKMQRKLLRLSALLEEADEGFLSLWESARESAMPVTVPSSP
                     DAAPAPSKAFDRASLKEYFEERGDVLRRWTDAAEETGFPPFIPSQEYLETSLSYLWDI
                     LRAAEMDSREPLDEFIRSLDEEGRGKEQISTIYRAFEKEASSWKVDGYSALFLLVLNM
                     KWDILQQKDLVELNIKKGSDRIKGVS"
BASE COUNT          942 a         1264 c         1158 g          917 t
ORIGIN      
        1 ggaaagatcc agcacccgtt tcgctctcgg gctgagggga agatccaccg gcttgaggat
       61 gggatgggat ttgccgaccg actcctcgat acggagtctc atctcgtgaa gattgatccc
      121 gagggattcc atggactgca ccgcagcacc ttccccttcg gcaagtatgc cgagaaggat
      181 gtgctccgtt ccgataacgt cgtgcccgag gcgaagagct tccctgtggg caagctggac
      241 tacttttttt cccctctcag tgaaaaactg ccacatgatt catcatcctt tgtctctccc
      301 ggtcagggag tctctccttt ggttcacgcc agggccaggc tccggagcat tctcttcaga
      361 agctctgcct gtatggcgtt ccttttgtag ggcgacacgt cgaattccga actcccgagc
      421 tcgtcggcgt gccggagggc gacctcgata aggatccgct cccgggaatc gatcatgccc
      481 cgttcctgga gcgatgtcag gaggcgcttt gcctcctggt cggtaacggc atcccccact
      541 atttcttcta tatggttgat tctttcctcc ggaatatcgt aacagagcct gaatatccgg
      601 atatatccat ggccgcctct ctggctttcc acgatatagc cccgctcggg ggtaaaacgg
      661 cttctgagga cgtaattgat ctggctgggc acacatccga agatttcggc cagctccttt
      721 ctccggagcg acacggcctg ttcgtccatc tcggaaaaaa ggttgttgat atagttttca
      781 atgactctcg tcagactcgc catggcactt ccctccagac gtaaaatttc ccgatgatta
      841 ttctacgcca ttggtcagca ttagtcaatg cgaatgggca tggtcgaaga tcctgacgag
      901 tcggggttcc tatttcttga agctgctttc ctttccttca aaaagacggc ggatgttgtc
      961 cctgtgccgt gtggtggaaa gggcggccag ggctgtcaga agggcgagga agggagcggg
     1021 gaaagagaga gcggcggcga tgccgggaag gcaccagaga gaaatgaggg acgcgagaga
     1081 aacatatccc ttcagtttca gcagggcaaa ccagatgaca cctcccgcag gggcaagaaa
     1141 aaaagagaga ggcgggtgga gaaaaaacac gacgccgtag gtggatgcga caccctttcc
     1201 tccccggaaa ccgagccaca gagggaaatt gtgaccgcag acaccggcaa aaccggtgaa
     1261 agcaatggtc catgaggatt caacaccggc ggcccgggcg cataaaatcc cgacggacgc
     1321 cttgatcata tccaaaagtg tgactgcaac cgcccatttt tttcccatga gccttcccac
     1381 gttggtcgca cccacattgc ctgaaccgaa ggtccgaata tccacccccc gaaatgcccg
     1441 ggctgcaaga tagcccgtgg gaaaggaacc ggcgaggtac cccagaagaa tccagaacag
     1501 gaaactcatt ttttccttcc tttcttttct atgaaggcat ccactacaag gacgcccttt
     1561 ttgttcacaa agagcggatt gacgtcgagc cgggccactt ccctctcgca gaatgccagt
     1621 ctggaaaagg cggagagtgc cgaggcaaaa gcaaccatat cgagaggatc tcttctccat
     1681 ccttccagca gcagcggata tcccctcgat tcccttatca tgcacaaagc ttctttttcg
     1741 tcgaaaggag caacccggat caccccgtcc ggcaaaattt ccgtataaac tccgcttatt
     1801 ccggtttcca caaggggacc gaaccgggaa tccctccgga attccatcct ccattcggtt
     1861 ccgcccctga tcatttcctg gacaaggact ccccttatcc tggcccgggg gttggcgacc
     1921 ctcgcttttt caagtgtcct gccgtaggcg ttcctcagtt cctcccggtc ctgcaaattg
     1981 agggcgacca cccttgcctc acttttgcga aggatgttcg gtgacatgac cttgagggcc
     2041 acaggatacc cgatctcctc cgcagcctcc gcagcttctc tgaaagacct gcagagtctc
     2101 tccctcgcca cgggtatgcc gaaagcgccg agaagaactt ttgcatcata ttccgtgggc
     2161 cgggggtgta tcgggcagcc gggcaggatg ggcttttccg tcccccccgg cattgttgcg
     2221 gcctcgggaa aaaaggtata ggaaagtgcg tccagggatc ttccgatgcc gggtatgagc
     2281 ggagtcttcg gatcttccgg accgcgtccg ttctcttccc ggggaaagga cagggaagag
     2341 gccaggaggc aaggcttttt tttatcaccg gtcttttccg ccatcggcct ccggggagaa
     2401 gtatgcacgc agttcctgag aaccttcagc accggagagg atccgtcgtc ctggtcgaga
     2461 aggggaattc ccctcctgga cgcttcggac ctgacgagcc gggccatttc tccctggccc
     2521 gcttcgagga gaatccccct gtgccgctct ccgccgcagg caagaaaagc cgctgctcca
     2581 tcaaccacct gggagggttc gtgaagggag acgataccga actgccggag ggcggactcc
     2641 ctgacccggt cttctccgga aagaggatca tactccagca gaccgacgca tttcccgcga
     2701 ccggccgctt ccgcggcaaa ctcgaggagg tccctgccct tgcggagacg ctcaaggaga
     2761 aagacaaaaa aacgaacctc cccatcaagt tcgagaatgg cccgggcagc ctctacggga
     2821 gaaaggtcgc tcttttcccc aaaagagaat gcgtatcgca tcggtaccga cctctcatgg
     2881 gactcgcaca ccgtccgctg gaagagatct cttccctctg cgagaagggc ggttttgctc
     2941 cgcccgtctc ctgacaggaa cggtgtaacc aggccaccgc tccccgggcc gatgatcttt
     3001 gaacccgttt tctttgcgag attccgtaca gcggcaggga catcccgggg ccatcccctt
     3061 cccagcacca caaaaggaat cccgtgggaa acgcattcct cgagaatacg ctttccacgg
     3121 ggtccgtcgt tttctccgat caggaccgtt tgaggcaaag cattctcctc cagggaggaa
     3181 agaggttcaa ggccggccag ggtcaaagga aaggaatctt cacccttccc ggccaggagt
     3241 gttttcccca ttccgggcag tctgtatttt tccttcaaca gcccactccc cctttgctcc
     3301 gcgggatatc aaaaagggga aggcatattc ggccctcccc tgtgaataca gataccgcag
     3361 cggcggaaaa tcaggatact cccttgattc tgtcggatcc cttctttata ttcagctcca
     3421 caaggtcttt ctgctgcagg atatcccatt tcatgttcag aacgagaagg aagagagccg
     3481 aatatccgtc caccttccag gaagatgcct ccttttcgaa ggccctgtag atggtggaaa
     3541 tctgctcttt cccccgcccc tcctcatcaa gggaccggat gaattcatcg aggggctccc
     3601 tgctgtccat ttcggcagcc cgcaggatat cccaaaggta cgaaagactg gtttcaagat
     3661 actcctggga aggtataaag ggcgggaagc ccgtttcctc cgccgcgtcg gtccatcgcc
     3721 ggaggacgtc accccgctcc tcgaaatact ctttaaggga tgcccggtcg aaagccttcg
     3781 acggagcggg agcggcatcg ggagaggacg gaacggtaac aggcatggcc gattccctgg
     3841 cggactccca tagagagaga aaaccttcgt ccgcttcctc aaggagagcg ctgaggcgaa
     3901 gcagcttccg ctgcatcttt ttcttcgttg atgtatcggt gggcagtgac accttggggg
     3961 catttgccgc ccagacttcc tggagtacgg tccggatctc gagggtgaac ggcaggtcct
     4021 tgtatttcgc ccattctctc agggcacccc gctccctgtt cagggaaaga acataggaca
     4081 ctgccggata gccgtaccgg tctggtgaat caagactgga aaggggaacg gaacgggaaa
     4141 ggtccgcaag aaattctttc tggacaatgt cttccacttt cgccacgtca tcggggaaat
     4201 tcacgagaat cttcaccctg cacaagtccg acatgccggc aagaaccgac gcatccgccg
     4261 tcccggcggt actgaggaat t
//
LOCUS       DAOTHD010000051         4244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_24714_length_4244_cov_3.190021, whole genome shotgun sequence.
ACCESSION   DAOTHD010000051 DAOTHD010000000
VERSION     DAOTHD010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24714_length_4244_cov_3.190021"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..650)
                     /locus_tag="P5219_01555"
     CDS             complement(225..650)
                     /locus_tag="P5219_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HRX25471.1"
                     /translation="MKILVSACLLGVNCRYDGGGALNNSVLALMERHELIPVCPEILG
                     GLPTPREPAEISVGRVRTISGIDVTEQYGRGAAESLRLALLFGCSAALLKERSPSCGA
                     GMVYDGTFSGKLVPGDGFTARLLRSRGIRVIPESGIGEL"
     gene            complement(806..1774)
                     /locus_tag="P5219_01560"
     CDS             complement(806..1774)
                     /locus_tag="P5219_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRX25472.1"
                     /translation="MLKGRKMILAVGVLALFLFSGAAFAVTHITMGTAGVGGMNYPVG
                     MAMAKIWNANIKDMKAVAIATAGAVQNIDMIRTKDIEVAVCRANEAYRAFNGIEKYEG
                     KPHSWLRALTGGVMFDAKTVVALKDEGIKSIKDFKGKRVAVGPVGSGGELDAREILAA
                     YGLTYKDITPEYVEASQAVDMMSDGLIQGAILGFTPGASAISELMVTGKVIILPIEEE
                     GYQALKKINPLIDKRVLKAGTYPNQDYDVETCGDPADLIITREDIPEDLVYQMTKSLY
                     ENLDGVRSVAAAVRGFGPDLVAEPDKMLIPYHPGALRYFKEAGILK"
     gene            complement(1848..3821)
                     /locus_tag="P5219_01565"
     CDS             complement(1848..3821)
                     /locus_tag="P5219_01565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HRX25473.1"
                     /translation="MAREFGVTECEPATQGEAVLRKATKECSYRTELAPFWTTAIIVL
                     SCVFILYHLITSRFGMPPMFKHRAIHLGFILCLVWMYYPASKRSIQSRPSTVDMVLIA
                     ASIAVCGYTVFNVDAFALRGGVALQRDYYFGAVAILLVLEACRRVAGKGLLILAIVFL
                     LYALLGRHIPGVLSHRGYTIERIIYQMYLTTEGIYGLPIGIAATYLVLFIVFSSFLEK
                     SGLGTLFNDVSLALGGRLVGGPAKVAVIGSGLVGMISGSAATNVATSGAFTIPLMKKI
                     GYKPYFAGAIEAAASTGGQIMPPIMGTGAFIMAEFLGVPYISIAAAAIIPAVLYFAAV
                     FFQVDLRARKIGLKGMAKEDLPDVKKTVLRYGHMFLPIVILVVLLMQHFTPLYAAFYS
                     ILVTWILSFLRKETRMGVDKLIPLAVSSARSCLSLSVAMANAGFVVAVLSMTGIGIIL
                     ADNIVALSHGSLFVTLVLCMVVSIVLGMGLPTSACYIIAATIAVPILHEMKVPGLQAH
                     FFVFYFACLSTVTPPVALASYVAAGMSGAGTNEVGWAAFKLALAGFIVPFFFIYAPAI
                     LLIADSAAVIAWAAVSGLLGAALLSVAVEGFLFIRLPWLLRIVFFAAALSLIAPGLNS
                     DLIGFGLTAAGLAGTLILRKKYGGTRDNEPGTM"
     gene            complement(4007..>4244)
                     /locus_tag="P5219_01570"
     CDS             complement(4007..>4244)
                     /locus_tag="P5219_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25474.1"
                     /translation="LPEIILMIGTSLLGAFALALGGSGYWMRNLNILERIIFFGAAVT
                     LIFPGVVTDSVGVGIMAVMWFLQKRSVKNLPQQS"
BASE COUNT          913 a         1204 c         1310 g          817 t
ORIGIN      
        1 gtctccaccg agatggggga aatcctcgtc caggtggaca acccggacgc ccggcagatc
       61 ttcgccgagg gagagcccgt ctcggtctcc ttcattccgg aactcaccaa ggcccttccg
      121 cccgaggaat aaaaggagga aaaagaagcc ccgggtccag aaacgtcaag ggggatgggg
      181 acatccggtc cctatccccc ttgacctgac ggtacagctc cccgctacag ctccccgatc
      241 ccgctttccg gaataacccg tattcccctg ctccggagca gcctggccgt gaaaccgtcg
      301 ccgggcacca gttttccgga gaaggttccg tcatagacca tgcccgctcc gcaggacggg
      361 cttctctcct tcagaagggc ggcggagcaa ccgaacagga gcgcaagacg aagcgactcg
      421 gcggctcctc tcccgtactg ctccgtcacg tcaattccgc tgatggtccg gaccctgccg
      481 acggatattt ccgcaggttc cctcggcgtg ggaagccccc ccagaatttc cgggcagacg
      541 ggaatcagct catgccgttc catcagcgca agaacgctgt tattcaaagc gcctcctccg
      601 tcatatctgc agttcacgcc cagaaggcac gcactgacaa gtattttcaa gcactggtct
      661 cccctttccg ttgttccgca ggaccgtgta ttgattatac ccgtttccgg caggagacca
      721 tcccgggggg tacggggcat ggggatgcaa aaaaaccccg gagattcccc ccggggtttt
      781 tcgcatctct tgaagcaggt ctgtcttact tcaggattcc ggcttccttg aagtagcgga
      841 gagcgccggg atgataggga atcagcatct tgtcgggttc ggccacgagg tcgggaccga
      901 agccgcggac tgccgccgcc acgctgcgga ctccatcgag gttctcatag agggacttgg
      961 tcatctggta aacgaggtcc tcggggatat cttccctggt gatgatgagg tcggcgggat
     1021 cgccgcaggt ttccacgtcg tagtcctggt tcgggtaggt tccggccttc aggactctct
     1081 tgtcgatgag ggggtttatc ttcttcaggg cctggtatcc ctcttcctcg atgggaagaa
     1141 tgatgacctt gccggtgacc atgagttcgc tgatggcgga ggcgccggga gtgaagccga
     1201 ggatggcgcc ctggatcagt ccgtcggaca tcatgtcgac ggcctggctg gcttccacgt
     1261 actcgggggt gatgtccttg taggtcaggc cgtaggccgc gaggatttcc cgggcgtcaa
     1321 gctcgccgcc gcttccaacg ggtccgacgg cgactctctt gcctttgaag tccttaatgg
     1381 acttgatgcc ctcgtccttc agggccacca ccgtcttggc gtcgaacatg acgccgccgg
     1441 taagggcacg gagccaggaa tggggcttgc cctcgtactt ctcgatgccg ttgaaggcac
     1501 ggtaggcctc gttggccctg cagaccgcca cttcgatgtc cttcgtccgg atcatgtcga
     1561 tgttctggac ggcgcctgcg gtagcgatgg cgacggcttt catgtccttg atgttggcat
     1621 tccagatctt ggccatggcc atcccgacgg gatagttcat tccgcccacg ccggccgttc
     1681 ccatggtgat gtgggttacg gcgaacgcgg cgccgctgaa caggaacagc gcaagaaccc
     1741 caactgccag aatcatcttt cttcctttca acatgtttca tcctcctttt gggctttgcc
     1801 cgtctttgtg ggattacccc acatttttgt gcatttgctg cggtctgtta cattgtaccg
     1861 ggttcattat cccgggtgcc tccgtatttc ttccgaagaa taagggttcc cgcaagccct
     1921 gccgcggtaa ggccaaaacc gatcaggtcc gagttgagac cgggggcgat gagggacaat
     1981 gccgccgcaa aaaagacgat gcggagcagc cacggaagcc ggataaacag gaatccctcc
     2041 acggcgaccg aaagcagggc tgcccccaga aggccggata cggctgccca ggcgataacc
     2101 gcggcgctgt cagcgataag caggatggca ggcgcgtaga tgaagaaaaa ggggacgatg
     2161 aaccctgcga gggccagctt gaaggctgcc cagccgactt cgtttgtccc ggcgccggac
     2221 atgccggccg cgacgtagga ggccagggcc accggagggg taaccgtcga aaggcaggcg
     2281 aagtagaaga caaagaagtg ggcctggaga ccgggaacct tcatctcgtg aagaatgggg
     2341 accgcgatgg tggcggcgat aatgtagcat gcgctcgtcg ggagccccat gccgaggacg
     2401 atggagacca ccatgcagag caccagggtg acgaagaggc ttccgtgtga aagggcgacg
     2461 atgttgtctg cgaggatgat gcctattccg gtcatggaca ggacggccac cacgaacccc
     2521 gcattggcca tggccacgct gaggctcagg caggaccttg ccgacgaaac cgcgagcgga
     2581 atgagcttgt cgacgcccat gcgggtttcc ttcctcagaa aggaaaggat ccaggtcacg
     2641 agaatcgaat aaaaagcggc gtagagaggc gtgaaatgct gcatgagcag gacgacgaga
     2701 atgacgatgg gcaggaacat gtggccgtac cgcaggacgg tcttcttcac gtcgggaaga
     2761 tcttccttgg ccatgccctt caggccgatt ttcctcgccc tgaggtcgac ctggaagaag
     2821 acggcggcga agtacagaac ggcgggaatg atggccgccg ccgctatgga gatgtatggg
     2881 acacccagga attccgccat gatgaaggct cctgtgccca tgatgggggg catgatctgg
     2941 ccgcccgtgg aggcagcggc ttcgatggcc ccggcaaagt agggcttgta gccgatcttt
     3001 ttcatgagag ggatggtaaa agctccggaa gtcgcgacgt tggtggccgc gctgccgctg
     3061 atcatgccca cgaggccgct gccgatgacg gccaccttgg cgggaccgcc gacgagcctg
     3121 ccgccgaggg cgagggagac gtcgttgaag agggtaccga ggccgctctt ctcaaggaag
     3181 gaggaaaaga cgatgaacag gacgagatag gtggccgcga tgccgatggg gaggccgtag
     3241 atcccttccg tggtcaggta catctggtag atgattctct ctatggtata tcctctgtgg
     3301 gaaagaaccc cggggatatg ccgcccgaga agggcataga gaaggaaaac gatggccagg
     3361 ataagcaggc cctttcccgc gactctccgg caggcctcca gaacgaggag aatggccacc
     3421 gcaccgaaat agtagtccct ctggagagcc acgccccccc ggagagcgaa ggcgtcgacg
     3481 ttgaagaccg tgtagccgca gacggcgatg gaagccgcga taagcaccat gtcaacggtg
     3541 ctgggccttg actggatcga ccgttttgac gcggggtagt acatccagac aaggcagagg
     3601 atgaagccga gatggatggc cctgtgcttg aacatgggcg gcatgccgaa ccgcgaggtg
     3661 atcaggtggt acaggatgaa aacgcaggac agcacgataa tcgccgtggt ccagaaggga
     3721 gccagttccg tacggtagct gcactccttg gtggctttcc gcaggaccgc ctctccctgg
     3781 gttgccggtt cgcactcggt gacgccgaat tcccttgcca ttcgattccc tccaaaatga
     3841 tgaactaaaa tgttgacaga gtgcccctta tatttgatgc ataatatatt ctatacgtac
     3901 tctccgggaa aagcaatatg tataatgtat cctgattttg gctccctccc aaaaaaaaaa
     3961 gcaggtcctg caaacaggac ctgctgcata ggcagaaaac ggaagatcag gactgctgcg
     4021 gcagattttt caccgaacgc ttctgcagga accacatgac ggccattatc ccgactccga
     4081 cggagtcagt cacaactccc gggaaaatga gcgtgaccgc cgctccgaag aagatgatcc
     4141 gctcgagaat gttgaggttc ctcatccagt atcctgagcc gccaagggcg agagcaaagg
     4201 ccccgagcag ggaggtcccg atcatcagga taatttcggg gagg
//
LOCUS       DAOTHD010000052         4236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_24773_length_4236_cov_3.424779, whole genome shotgun sequence.
ACCESSION   DAOTHD010000052 DAOTHD010000000
VERSION     DAOTHD010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24773_length_4236_cov_3.424779"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /locus_tag="P5219_01575"
     CDS             complement(<1..1069)
                     /locus_tag="P5219_01575"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRX25475.1"
                     /translation="MIYSDFDPKPVFREYRRLIGDGEVGGKARGLAFAFNTLKGTPLE
                     SCVEFPDVNYVLTTEGFDDFVSDNGIETLLKESLSGQEENTEDEFARELFEKVASAFR
                     NGKVRPSLGRDLEDAMEAIGDFPLAIRSSSILEDSRKLSFAGKYSTRFSANRGPREER
                     SVLLVNAIKEVWASLYNPAARAYRKKHGLTDSDESMAVVIQPVIGREHNSMYYPEIAG
                     TAFSKVYRRPSTRIRKEDGVMRFCFGLGTRTVDRLKANVSYLSHPMLRPQGNLPADIA
                     MTSQSEFDYIDRASGKFMTGSLADHLSFLLREHRLASAFIEIYAENLLYWAGSDQVSN
                     GKPVFSFSNFPRRHPRFFSLVK"
     gene            complement(1106..2407)
                     /gene="asnS"
                     /locus_tag="P5219_01580"
     CDS             complement(1106..2407)
                     /gene="asnS"
                     /locus_tag="P5219_01580"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRX25476.1"
                     /translation="MTAPWTYIKDLPSREGADVTVRGWLYNMRSSGKIHFLQLRDGTG
                     TVQGVMVKKEVSEEDFSAAKSLWLEASVEVTGTVRKDDRAPSGVELSVASVSVIQNPA
                     DEYPIGKKDHGIDFLLDNRHLWLRSSRQQAIMRIRERVIWSAREFLHNDGYLLVDSPI
                     ISGAAGEGVSGLFEIDYFDSKAYLAQTGQLYAEAAAAAFGRVYCFGPTFRAEKSKTRR
                     HLTEFWMLEPEVAFFDHKDNMDLQERLICHIVSSVLEHCGNELAALERDVSKLENIKA
                     PFYHLQYGEAIEMLKKLGSDTLYGEDLGNDDETILTQQFDRPVFIECYPKSVKAFYMK
                     EHPENQDLVLCDDLLAPEGYGEIIGGSQREDSYERLVARIREQGLPEEPYDWYLDLRR
                     YGTFVHSGFGMGIERVVAWIAGLQHIREAIPWPRTIYRLKP"
     gene            2513..3892
                     /locus_tag="P5219_01585"
     CDS             2513..3892
                     /locus_tag="P5219_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25477.1"
                     /translation="MIIPRLVIADERREGTVPPGVILAAALKSAGHPLRIFCAGPDEY
                     LVRLTTLVTGEEVTVLDPFSCVTARVLKTLFQFASDRERLNIILAPLGQRGDDGGFSL
                     FPQGPELSRVLECPLIPVMFADSSATIIARVLEKAVSGIAAHGEIPVPAVLFASVLNP
                     REFQLLEIEAGRRAPLLSLGYIPKLLERNRSTLLELCLDAMAERAAFPVKAAASQLAS
                     LPGQIDWNAFWGFARLNQEWTEVAEPFRGKAAGLTVGVVTHSGLDLEGDNARRLFSYL
                     GCSVRKIFLEETRPALDVNAVYVPHGLGFLSAEKILAAQGAREWFSGLFKSRKVVFVN
                     GGAAPLLGESFALPNGKQYEGMNIFHYRGKYDMPSEDLKKVEVSSLEGDILLNLGEKL
                     RGYLPSYASVINPGDAAQSLWSVKDPGNGKEKGFSGWNKGYGVVTDLCIELWSNVEGI
                     YRWLVLRKK"
     gene            complement(3896..>4236)
                     /locus_tag="P5219_01590"
     CDS             complement(3896..>4236)
                     /locus_tag="P5219_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRX25478.1"
                     /translation="WSDLPVVSAIYTGRPRSELSLALKLIGWTDFPGDLTITPDDGIT
                     KPSPLGLSVLCEKTGASFPLFLGDAESDRKTVSAFGRGNFAAIGDFLPDEAKRYKTPE
                     EALRDKGLLG"
BASE COUNT          889 a         1153 c         1217 g          977 t
ORIGIN      
        1 ctttcacgag gctgaaaaag cgggggtgcc gcctcgggaa gttcgagaag gaaaataccg
       61 gcttcccgtt gctcacctgg tcagagcctg cccagtagag gaggttttcg gcataaattt
      121 cgatgaacgc ggaagcaagc ctgtgttccc tgagcaggaa ggaaaggtgg tcggccaggg
      181 agccggtcat gaacttcccg gaagccctgt cgatatagtc aaactcgctc tggcttgtca
      241 tggcgatgtc tgcgggcagg tttccctggg gcctgagcat ggggtgggaa agataggaga
      301 cattggcctt cagcctgtca accgtcctgg ttccaagccc gaagcagaac cgcatgaccc
      361 cgtcctcttt ccgtatgcgg gtggaggggc ggcggtagac tttcgagaag gcggtgcctg
      421 cgatttccgg gtaatacatt gaattgtgct ccctgccgat aaccggctga ataacaactg
      481 ccatggactc gtccgaatcg gtgaggccgt gtttcttccg gtaagccctg gccgccggat
      541 tgtacaggga tgcccagact tcttttatgg cattgacaag gaggacggaa cgctcttccc
      601 ggggcccccg gttggctgaa aaacgtgtgc tgtatttccc tgcgaaggaa agcttcctcg
      661 aatcctcaag aatggagctg gaccttatgg caaggggaaa gtcgccaatg gcttccatgg
      721 cgtcttccag gtccctgccc agggacgggc ggacctttcc gttccggaag gcggaggcga
      781 ctttctcgaa aagctcccgg gcgaattcgt cctccgtgtt ctcctcctgc ccggagaggc
      841 tttccttcag aagtgtttct atcccgttgt ccgaaacgaa atcatcgaac ccctccgtgg
      901 taagcacata gtttacatcg gggaattcga cgcaggactc gagaggcgtt cctttcaggg
      961 tattgaaggc aaaagcgagg cccctggcct tgcctcccac ctcaccgtca ccgatgagcc
     1021 tcctgtattc gcgaaaaacg ggtttcgggt caaaatcgga ataaatcacg tactgtgcct
     1081 ccctgcgaaa cggaaaatga atgcatcagg gcttgagacg ataaatcgtc cgcggccagg
     1141 gaatggcttc cctgatgtgc tgcagtccgg caatccacgc caccacccgt tctatcccca
     1201 tgccgaaccc gctgtggacg aacgtcccgt acctccggag atcaaggtac cagtcataag
     1261 gctcttcggg aaggccctgt tcccgtatcc tggccacgag ccgctcgtaa ctgtcctctc
     1321 tctgggagcc tccgatgatt tcgccgtatc cctcgggagc gagaaggtcg tcgcagagca
     1381 ccaggtcctg attttccgga tgttccttca tgtagaaggc cttgacgctc ttcgggtagc
     1441 attcgatgaa caccggccgg tcgaactgct gggtgaggat ggtttcgtcg tcgttcccga
     1501 ggtcttcacc gtagagagtg tcgcttccca gcttcttcag catctcgatg gcctcgccgt
     1561 actggagatg gtaaaacggg gccttgatgt tttccagttt tgaaacgtcc cgttcgaggg
     1621 cggccagctc gtttccgcag tgttccagaa cggatgagac gatgtggcag atgagccttt
     1681 cctggaggtc catgttgtcc ttgtggtcga agaaggccac ctcgggttca agcatccaga
     1741 attcggtcag gtgacggcgg gtttttgact tctccgccct gaacgtgggg ccgaagcagt
     1801 aaaccctgcc gaaggcagcc gcggccgcct ctgcatacag ctgtcctgtc tgggcaaggt
     1861 acgcctttga atcgaagtag tcgatttcaa acaggccgga aactccctct cctgccgctc
     1921 cgctaatgat cgggctgtcc acgagaaggt agccgtcgtt gtgaaggaac tcccgggcgg
     1981 accatatgac cctttcgcgt attctcatga tggcctgctg cctgctgctg cggagccaga
     2041 ggtggcggtt gtccaggagg aaatcgattc cgtggtcttt tttgccgata ggatattcgt
     2101 cagcgggatt ctggataacc gaaacggacg ccacggaaag ctccactccc gacggagcac
     2161 gatcatcctt tctgaccgtt cccgtcactt ccacagaagc ttcaagccac agggattttg
     2221 ccgcagagaa gtcttcttcg gagacttctt tcttcaccat gacaccctga actgttcccg
     2281 ttccgtccct gagctgcaga aagtgaattt tgcccgagct tctcatgttg tacagccaac
     2341 cgcggacagt gacgtccgct ccttcccggg aggggaggtc ctttatatag gtccatggtg
     2401 ccgtcatgcg caatctacct ccagacattg agacgagtat gaattttgac cgtcgaggct
     2461 catggcgtat atgatacact aaaccatacc gtaaaaaaaa ggagaagcgg ccgtgattat
     2521 tccccgtctt gttatcgccg acgaaagaag ggaaggaact gttccgcccg gcgttattct
     2581 cgctgcggct ctgaagagcg caggtcatcc tctgaggatt ttctgcgccg gtcctgacga
     2641 gtatctcgtc cgattgacca ctctggtcac gggggaggag gtaacggtgc tcgatccctt
     2701 ctcctgcgtg actgccaggg tattgaagac gctttttcag ttcgcgtcgg acagagagcg
     2761 tctgaacatc attcttgctc ccctcggaca aagaggggat gatggcggtt tttcactctt
     2821 tccccaaggc cccgaactca gccgtgttct tgaatgtcca ctgataccgg tgatgttcgc
     2881 cgattcctcg gcgacaataa tcgcccgcgt tctcgaaaaa gcggtttccg gaattgccgc
     2941 ccatggagag attcccgttc ccgcagttct tttcgcttcc gttctcaacc caagggaatt
     3001 ccagcttttg gagattgaag caggaagaag agcccccctt ctcagccttg gttatatacc
     3061 gaaactcctt gagagaaaca ggtcgaccct gttggagctt tgtcttgacg ccatggcgga
     3121 gagagccgcg tttcccgtca aagccgctgc gtcgcagctg gcgtcactcc ccgggcaaat
     3181 cgactggaac gccttttggg gattcgccag gctgaaccag gaatggactg aggtggcgga
     3241 gccattccgc gggaaggcgg cagggcttac cgtgggagtg gttacccaca gcggactcga
     3301 tcttgaaggc gacaacgcga gaaggctttt ttcctacctg gggtgttccg tgcggaagat
     3361 ctttctcgag gaaaccagac cagcccttga cgtcaacgcc gtctatgtcc cccacggcct
     3421 gggttttctc agcgcggaaa aaatccttgc agcacaggga gcgagagaat ggttcagcgg
     3481 gcttttcaag tcaagaaagg tcgtttttgt caacgggggg gcagcccccc ttctcggtga
     3541 atcctttgca ctcccgaacg gaaagcagta tgaagggatg aatattttcc attacagggg
     3601 aaagtacgat atgccctcag aagatctgaa aaaagttgag gtttcttctc ttgaaggaga
     3661 tattcttctg aatctcgggg aaaagctcag ggggtatctt ccttcctatg cgtcggtcat
     3721 taaccccggg gacgccgccc agtcgctctg gtccgtcaaa gacccgggaa acgggaaaga
     3781 aaaggggttt tccggctgga acaaaggata cggagtggtg actgatctct gcatcgaact
     3841 ctggagcaac gtggagggaa tttaccggtg gctcgtgctg agaaagaaat gagccttatc
     3901 ctagaagtcc cttgtcccgg agggcttcct ccggggtctt gtatctcttc gcctcgtcgg
     3961 ggaggaaatc gcctatggct gcgaagttac ctcttccgaa ggcggaaacc gtctttctgt
     4021 cgctttcggc atctccgaga aagaggggaa aggatgcccc ggttttttcg cagagaaccg
     4081 acagtcccag gggagagggt ttcgtgattc cgtcatccgg ggttattgtc agatctccgg
     4141 gaaagtccgt ccagccgatg agcttgaggg caagggaaag ctctgaacgg ggccttccgg
     4201 tgtagatagc cgaaacgacg ggaagatcgc tccagt
//
LOCUS       DAOTHD010000053         4200 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_25058_length_4200_cov_3.531242, whole genome shotgun sequence.
ACCESSION   DAOTHD010000053 DAOTHD010000000
VERSION     DAOTHD010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25058_length_4200_cov_3.531242"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1345
                     /locus_tag="P5219_01595"
     CDS             <1..1345
                     /locus_tag="P5219_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HRX25479.1"
                     /translation="RGIADTDLSDPVVEAVGKAFGTWLRRRGVDKVSVGGDVRLSTAR
                     IERAAVRGLTWAGLDVVRLGTVTTPMLYWSIPGLSLGGGVMITGSHNPKDMNGLKLSC
                     GTSTLYGDEIREILRMILEDDLQKGSAEGRVEKADISGRYLEMLQSKITLGSRKLKVV
                     ADCGNGTAGLHVRPFLESLGCTVVPLFEKPDGTFPNHHPDPQKRENLLSLMETVVREK
                     ADLGIAFDGDADRLGVVDERGEMIWGDILMALFWEEILPKHPGAEAIVEVKCSQSLVD
                     EILRLGGKPLFWKSGHSLIKAKMKEIGAVFAGELSGHFFFADEYYGFDDSFYAAGRLL
                     RMLSHGTESLSAMISHVPVYLSTAEIRIDCPDEEKFAVVDAIRDKALKDHEAITVDGV
                     RILYPEGWGLVRASNTQPVIVTRCEGRTGKDLEEIGADVKKRLLAEGLPDFEWTF"
     gene            complement(1369..2073)
                     /locus_tag="P5219_01600"
     CDS             complement(1369..2073)
                     /locus_tag="P5219_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HRX25480.1"
                     /translation="MENGRGKTKIYDQVVETVKKMIADGTLPQGSPLPPERQLIEELN
                     VSRSSLREGFRILEMMGFIESIPGKGRFVRRSRGEGGSSGKVPLQDEAILELVEARLV
                     LEPAIAVEAAKHASPSDLTRIRRILTKTGKDVESLEHRAQCDYDFHLVLAESTHNFIF
                     VNIVKTTFNLIMATHERIYALLDDKEIFLREHEDVYDAIVVRDLDRVTRLMTAHVQRV
                     YRTLQNGIALEGIREI"
     gene            2184..3827
                     /locus_tag="P5219_01605"
     CDS             2184..3827
                     /locus_tag="P5219_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HRX25481.1"
                     /translation="MAFTVHVKNGPVFSSDVPVTGKAVLSESRPEGGDTVVAWRVNNY
                     LRSLDWVIEDSAEVEFIDTSSFEGMEVYRRSLSFLLVLACRKALGRNIVLRHSISEGY
                     YWEDQDGPVSGDDLQAIKSMMSDLVAKDLPFVRKVVSLDKARKIFERQGSCEVARLFK
                     WAGVDPVELYRCADIYGYYYAPLAPSTGYLKLFDLKPLGPGMVLQFPTINYPSALPPF
                     RASKNLSSVFLDYARWLEVLDLRTMDSLHKKVADGKSLEVILISEAFHARNLGKIAEE
                     IDSRPKVKVVAIAGPSGSGKTTLSERLKIQLQVCGKNPVTLAMDNYFVDRENTPKDEE
                     GNYDFEVIEALELELLEDHLKRLLAGEEVVLPQFDFIEGKKFPGKKVRLQPRDILIME
                     GIHGLNDRITDVIPKDERYGIFVSPLTGVYLDDHNRTSTTDNRLLRRLVRDSRTRGHS
                     AESTLTIWPKVIRGAMKYVFPYQKRADIMFNSSLPYELSALRAYVDPILHTVPEGSPV
                     YGDAQRLLSMLRFIPMIPSENIPNNSIIREFIGGTCFDA"
     gene            3840..>4200
                     /locus_tag="P5219_01610"
     CDS             3840..>4200
                     /locus_tag="P5219_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRX25482.1"
                     /translation="MAKATMEDVARLAGVNKATVSRALRGDSRISQATREKVWKFAKE
                     IGYRLDLAASTLSGGRTGIAALVLEEIDPWLTGQFLEGLNRVLSRSGLDLLLKLPGFH
                     PGELLSSLEARHVDCILW"
BASE COUNT          940 a         1160 c         1173 g          927 t
ORIGIN      
        1 ccgcggtatc gctgatacgg acctttccga ccctgttgtc gaagctgtgg gaaaggcttt
       61 cggcacctgg ctccgccgcc gcggggtgga caaagtttcc gtgggcggcg atgtccggct
      121 ctcaaccgca agaatagaac gggctgcggt gcgcgggctg acctgggccg gactcgacgt
      181 ggtcaggctt ggaacggtta ccacgcccat gctgtactgg agcataccgg gactctctct
      241 cggcggagga gtcatgataa cgggaagcca taacccgaaa gacatgaacg gcctcaagct
      301 ttcctgcggt acttcgacac tgtacggcga cgaaattcgg gagattctcc ggatgatcct
      361 cgaggacgat cttcagaaag gttccgctga aggcagggtg gaaaaagcag atatttccgg
      421 caggtacctg gagatgctgc aatcaaaaat cacactgggt tcccggaaac tcaaggttgt
      481 tgccgattgc gggaacggga ccgccggcct tcacgtccgt ccttttctcg aatctttggg
      541 atgcaccgtg gttccgcttt tcgaaaagcc tgacgggaca ttccccaatc accaccccga
      601 tccccagaag cgggaaaatc tcctttccct catggagacc gtcgtcaggg agaaggcaga
      661 cctcggcatc gctttcgacg gagacgccga cagactggga gtggtggacg aacggggtga
      721 gatgatctgg ggagatatcc ttatggctct tttctgggaa gaaatccttc cgaaacatcc
      781 gggagcggaa gccatagtgg aggtaaaatg ctcccagtcc cttgtggatg aaatcctcag
      841 gctcgggggg aagccgctgt tctggaaatc gggccattcc ctcatcaagg cgaagatgaa
      901 ggagatcggt gccgttttcg ccggtgaact atcgggccat ttctttttcg cggacgagta
      961 ttacggtttt gacgactctt tctatgcggc gggaagactc cttcggatgc tttcccacgg
     1021 cacagagagc ctttcggcca tgatctccca tgtaccggtc tacctgagca ccgcggaaat
     1081 caggatcgac tgccccgacg aagaaaagtt cgccgtggtg gacgcaatac gggacaaggc
     1141 attgaaggac cacgaagcca ttactgtgga cggggtgcgg attctgtacc ctgaggggtg
     1201 ggggctggtc agagcctcca acacccagcc cgtgatcgtt acccggtgtg aaggaagaac
     1261 cgggaaagat cttgaagaaa tcggggccga tgtgaaaaaa aggctcctcg ccgaagggct
     1321 gcccgatttc gaatggactt tctgaaaaaa gtcgggaaag ggagatcgtc agatctcccg
     1381 tatcccctcg agggcgattc cgttctggag cgtgcggtac accctctgca catgggcggt
     1441 catgagccgg gtcaccctgt caaggtccct gacaacgatg gcgtcgtaca catcctcgtg
     1501 ttccctcagg aaaatttcct tatcgtcaag gagggcgtag attcgttcat gagtggccat
     1561 aatgaggttg aaggtcgtct tcacgatatt gacgaaaatg aagttgtggg ttgactcggc
     1621 aagaacgaga tggaaatcgt agtcgcactg ggcgcgatgt tccagggatt caacgtcttt
     1681 tccggtcttt gtgaggatcc gcctgatcct cgtcaggtcc gaaggtgatg catgttttgc
     1741 cgcctccacc gcgatggcgg gttcaagaac gagccgggct tccaccagtt ccagaattgc
     1801 ttcgtcctga agaggaacct ttcccgaact tcccccttcg ccccgtgacc ggcgcacgaa
     1861 acgtcccttc ccggggatgc tttcgataaa gcccatcatt tcaaggatcc ggaaaccctc
     1921 ccttagagaa ctccggctta cgttcagctc ctcgatgagc tgccgttcgg gaggaagggg
     1981 cgatccctgg ggcagggttc catcggcgat catttttttc accgtttcaa cgacctgatc
     2041 gtaaatcttc gtttttcctc taccgttctc cataggcttc tcctcacagt gtaatggaca
     2101 cattgggata tgataagata cagcatagtc cactttattc atcaaggcaa catccttttt
     2161 cccaaccgag aggaggagac atcatggcgt ttaccgtcca tgtgaaaaac gggcccgttt
     2221 tttcgagcga cgttcccgtt acaggaaaag ctgttctctc cgaaagccgc cccgaagggg
     2281 gagacactgt cgtggcctgg agagtgaata attacctcag gtccctcgac tgggtcatcg
     2341 aagattcggc ggaggtcgag ttcatcgata cctcgtcttt tgaaggaatg gaagtctacc
     2401 ggcgctccct cagttttctg cttgtcctcg cctgccggaa agctctcggc cgcaacattg
     2461 tcctcaggca ttccatcagt gaagggtatt actgggaaga ccaggatggt cccgtttctg
     2521 gtgatgatct gcaggccatc aaaagcatga tgtcggacct tgtagccaag gatcttccct
     2581 tcgtcaggaa agtcgtctcc ctcgacaagg cccggaagat ttttgaacgg cagggaagct
     2641 gcgaagtggc gcggctcttc aaatgggcag gggtggaccc cgtcgaactg tatcgctgcg
     2701 ccgacatata cggctattac tacgctcctc ttgccccgtc cacgggatac ctgaagcttt
     2761 tcgacctcaa gcctctcggc ccgggaatgg tgctacagtt tccgacaatc aactatcctt
     2821 ccgcccttcc tcctttccgg gcgtcgaaaa acctttcctc tgtgttcctc gactacgcac
     2881 ggtggcttga ggtgctcgac ctgcggacca tggacagcct ccacaaaaaa gtcgcggatg
     2941 gaaagtctct cgaggtcatt ctcatttctg aggctttcca tgccagaaac cttgggaaga
     3001 tcgcggagga aatagactcc aggccgaaag tcaaggttgt cgccattgcc ggcccctcgg
     3061 gatcggggaa aaccacactt tcggaacggc tgaagataca gctccaggtc tgcggaaaaa
     3121 atcccgtaac cctcgcaatg gacaattatt tcgtcgaccg tgagaacacc ccgaaggacg
     3181 aagagggcaa ctacgatttc gaggtaatcg aagctctgga actcgagctt ctcgaggatc
     3241 atctgaagcg ccttctcgcc ggagaggagg ttgttcttcc ccaattcgac tttatcgagg
     3301 gaaagaagtt tccggggaaa aaagtacgcc ttcagccccg ggatatcctt atcatggagg
     3361 gcattcacgg tctcaacgac aggattaccg acgtcatccc gaaagatgag cgctacggga
     3421 ttttcgtatc ccccctgaca ggcgtctacc ttgacgatca caaccgtaca agcacaaccg
     3481 acaacaggct tctgaggaga ctcgtacggg attccagaac ccgggggcat tccgctgaat
     3541 cgacgctcac catatggccc aaggtcataa ggggagccat gaaatatgtc tttccctacc
     3601 agaagcgggc ggacatcatg ttcaattcat cccttcccta tgaactctcg gcccttcgcg
     3661 cctacgtgga tcccattctg cacaccgttc cggaaggctc tcccgtctac ggagatgccc
     3721 agcgccttct ttccatgctt cggtttatcc cgatgattcc gtccgagaac atccccaaca
     3781 actccataat cagggaattc atcggcggaa cctgcttcga cgcatagcgg ggcgctggga
     3841 tggcgaaggc gaccatggaa gacgtcgccc gcctggccgg tgtgaacaag gccacggtca
     3901 gcagggcatt gcggggtgac tcccgcatat cccaggcaac aagggaaaag gtctggaaat
     3961 ttgcgaaaga aatcggctac aggcttgacc ttgctgcgag cacgctgtca gggggcagaa
     4021 cgggaatagc agcgctcgtt ctggaagaaa tcgacccctg gctgacgggg cagttcctgg
     4081 agggattgaa cagggtactc tcaagatcag gcctggatct gctgctgaag cttcccggtt
     4141 tccaccccgg agaacttctt tcctccctcg aggcgcggca tgtggattgc atcctctggg
//
LOCUS       DAOTHD010000054         4187 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_25155_length_4187_cov_4.116893, whole genome shotgun sequence.
ACCESSION   DAOTHD010000054 DAOTHD010000000
VERSION     DAOTHD010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25155_length_4187_cov_4.116893"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="P5219_01615"
     CDS             complement(<1..760)
                     /locus_tag="P5219_01615"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRX25483.1"
                     /translation="MLGIGIIAPVLPLYAKRLGASGLAIGTIIGAFSFSRSGGMLISG
                     ELAERMNRKVLLLAGLAFYALASVAYTFASTTESLIAIRIGHGIGSAMVVPITMAIGA
                     EVVPKGKEGLFFGSLQGALFLGVGSGPLLSGLLAETIGFNAPFYAMTSLTVLSMILVF
                     RFLPGNLPSGRGSETLCGLRSVFQAILTDHEMLVVFFFQFCSAMCRGVLVMMIPLLAS
                     DIRLSLSEIGFVVSLNSLSTGVLQRFSGRLADNLH"
     gene            complement(1125..2129)
                     /locus_tag="P5219_01620"
     CDS             complement(1125..2129)
                     /locus_tag="P5219_01620"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRX25484.1"
                     /translation="MKSRHATVQLCRLLRESEGNIVSGGHVLRVLSLSRQAVWKAVRD
                     LEEEGFVVDSVPQKGYILRELPSNDLSPSLIGSLISPECPWGEEIHVFQSIPSTQEAA
                     KRIGRQSQTDGLVVIAEEQTNGRGRRDRVWISPRGTGLYFSVFFRPRMLPGRLQLINL
                     AAGLAVRDAIGAVCGKPVSLKWPNDLLYGGRKICGILSEASSDPERIRDCSTGIGINI
                     SLTREQLAVRGLENAASLGAGTETVHRGSLCAAVIEKFYRLAADLANDGGYSLLSRYR
                     EECSTIGKNVSVLTEEDAFSGRAAGIGDNGELLVEGKDGIRSFCAADVVHATPDVKDR
                     "
     gene            2208..3309
                     /gene="prfB"
                     /locus_tag="P5219_01625"
     CDS             join(2208..2279,2281..3309)
                     /gene="prfB"
                     /locus_tag="P5219_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_133956741.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRX25485.1"
                     /translation="MAITSNATVLEELRVLRKDLQDSLDLDSIRERKDKLEASASAPD
                     FWNREDCHGVTREISKLQRKLDTWDRVDGVFEDLTVLSEMLSESDDDELQSEFTATSE
                     QLRKIIEKEQLRLLLSEEYDGNNAILTVHAGSGGLDSQDWAEMLLRLFLRWAEREDFR
                     TKILEILQDEEAGIKSATVLVEGEFAYGYLKSEKGVHRLVRISPFDAAKRRHTSFASV
                     DVAPELPDDVEIEIRPEDLKMDTYRASGAGGQYVNRTDSAVRITHLPTGIIVACQNER
                     SQHMNRQVAMQVLRSKLFERAWQERQQELLAIQGEKKEISWGSQIRSYVLHPYTLVKD
                     HRTGHETGNVQAVLDGDIDGFIMNYLRWKKQS"
     gene            3460..3612
                     /locus_tag="P5219_01630"
                     /pseudo
     CDS             3460..3612
                     /locus_tag="P5219_01630"
                     /inference="COORDINATES: protein motif:HMM:NF017400.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3619..>4187
                     /locus_tag="P5219_01635"
     CDS             3619..>4187
                     /locus_tag="P5219_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25486.1"
                     /translation="MSGSPVYINGKLIGAVGYGWNFSDHNLGLVTPYEDMASVWDWPE
                     KIVTLPGLPPSGIEPQDEEDDDKKKSSPRNETPEKSGSGNTDEPVLKGELSPLFIDGI
                     SGRTAAEITALLGKERFIPGGSFSEDIPVEMNARLVPGEAITVLLAWGDVTIGSTGTL
                     TAVAPDGRFIGFAHPFLGRGAVNYPVARA"
BASE COUNT         1074 a         1083 c         1117 g          913 t
ORIGIN      
        1 tgtgcagatt atccgcaagt ctgccggaaa accgctgaag cactccggtc gatagagagt
       61 tcaaggagac tacgaatccg atctcggaaa gggaaagacg aatgtccgag gcgaggagag
      121 gtatcatcat cacgagaaca cccctgcaca tggcagagca gaactggaag aaaaaaacga
      181 ccagcatttc atgatccgta aggattgcct gaaaaacgga tcttaatccg cagagagttt
      241 cgctccccct gcccgatggg aggttgccag gcaggaaacg aaagacaagg atcatggaaa
      301 gaacggtaag ggatgtcatg gcatagaaag gggcattgaa acctattgtc tccgcgagaa
      361 ggccgctcaa cagaggccct gagccgaccc caagaaaaag ggctccctgg agggaaccga
      421 aaaaaagccc ttctttccct ttcggtacca cctccgcccc gatagccatc gtaatgggta
      481 caaccatggc tgaacctatc ccgtgaccta ttctgatggc gataaggctt tccgtcgttg
      541 aggcaaatgt ataggcaact gaagcaagag catagaaggc aagaccagcg agaagaagca
      601 cttttctgtt cattctttcc gccagttccc cggagatgag catcccgccg cttcgggaaa
      661 aggaaaaagc cccgataatc gttccgatgg caaggccgct cgcccccagc cgtttcgcat
      721 aaagggggag cacaggggca atgatgccta tgcccagcat ggcacaaaac atcgaaaggc
      781 tgaggacggt caaaatttta cggtaggaac cctctgtcga taacaacaat aatgcactcc
      841 gttcgttcca tgttgatttc ttatccgcat ttttattcat tgagcattat acgatgtttt
      901 gtctacaatt tcaacgcttc tccggattat gggtaatcca tgcgatcctc cacggggaaa
      961 ggctgcattt tttctgccgt ctttttttcg ctccggaaca aacgggacac aacggaaaaa
     1021 agcgtgaatc ccttttcaga attcacgccg gatcattgga gagacatcaa tgacttccac
     1081 tccttttttt tcacggaggc aagcggggag cccgcaatac gattttacct gtctttcacg
     1141 tcaggagttg cgtggaccac atcggcggcg caaaaggacc gtattccgtc ctttccttcc
     1201 acaagaagct ctccgttgtc tccaatacca gcagctcttc cggaaaaagc gtcttcctct
     1261 gtcaggacag atacgttctt ccctatggtg gagcattcct ccctgtagcg ggagaggaga
     1321 gaatatcccc catcattggc gaggtccgct gcaaggcgat agaacttttc aatgactgcc
     1381 gcgcagaggc tgcccctgtg gaccgtttcg gttcccgctc ccagtgatgc ggcattttcg
     1441 aggcctcgga ccgcaagttg ctccctggtc agggaaatgt tgatgccgat tcctgtgctg
     1501 caatcccgga ttctttccgg atcgcttgaa gcctcggaaa ggattccgca gatttttctc
     1561 ccgccataca gaaggtcatt cggccatttg agggacaccg gtttgccgca aacggccccg
     1621 atggcatccc ttacagcaag tccggcggcc aggttgatga gctgcagccg tccgggaagc
     1681 atgcggggcc tgaaaaagac cgaaaaatag agtcccgtgc cccggggaga aatccacact
     1741 ctgtctcttc ttcccctgcc gttcgtctgc tcctcggcaa tgacgacaag cccgtccgtt
     1801 tggctctgtc ttccgattct tttcgctgcc tcctgtgttg acgggatgct ctgaaaaaca
     1861 tggatttcct ctccccaggg acattccggt gaaatcaggc ttcctatcag agaaggagaa
     1921 agatcgtttg aagggagttc ccggaggatg tagcccttct ggggaacaga gtccaccacg
     1981 aaaccttctt cctcaagatc gcggacagcc ttccaaacgg cctgccgtga aagagaaaga
     2041 acccgtaaga catgacctcc tgaaacaata tttccctcgg actcgcggag aaggcggcag
     2101 agttgaacag ttgcatgtcg ggatttcatg gaattgcctc cttatcttcc ttactgaatg
     2161 atataatgaa aaatcgcgca gcacaataac gatgaggggg aattgacatg gcgataactt
     2221 caaacgccac tgttctggag gaactgcggg ttttgagaaa agacctgcag gacagtcttt
     2281 gacctggatt caataagaga aagaaaggat aagctggaag catcagcatc agcaccggat
     2341 ttctggaaca gggaagactg ccacggcgtc accagggaaa tttcaaaact ccagcggaag
     2401 ctggatactt gggacagggt tgacggcgtt tttgaggatc tgaccgttct ctcagaaatg
     2461 ctttccgaat cggatgatga tgaactccag agcgaattta cagctacttc ggaacaatta
     2521 aggaaaatca tcgaaaagga gcagcttcgt ctgcttcttt ccgaagaata tgacggcaac
     2581 aacgcaattc tcaccgtcca cgcgggttca ggcggtctgg actcccagga ctgggcggag
     2641 atgcttctca ggcttttcct tcgttgggcg gaaagggaag atttccgaac caaaatcctt
     2701 gaaatcctcc aggacgagga agcaggcatc aagagcgcca ctgtccttgt ggagggagag
     2761 tttgcctacg gctacctgaa gtcggaaaaa ggggttcacc gtcttgtaag aatttctcct
     2821 ttcgacgcag ccaagcgccg acatacaagc ttcgcttcag tggatgtggc gccggaactg
     2881 ccggatgatg tggagataga gattcgcccc gaggatctga aaatggacac gtacagagcg
     2941 agcggggcag gagggcagta tgtcaacagg acagattccg cagtaaggat aacccacctt
     3001 ccgaccggaa ttatcgtcgc ctgtcagaac gaaagatccc agcacatgaa caggcaggtt
     3061 gccatgcagg tacttcgaag caaacttttc gagagagcct ggcaggaaag acagcaggaa
     3121 ctcctggcaa ttcagggaga aaagaaagag atcagctggg gaagtcaaat acggagttat
     3181 gttctccatc cttacaccct ggtgaaagat caccggacag gacacgaaac gggcaacgtt
     3241 caggctgttc tcgacggcga tattgacggg tttattatga actatctgcg ctggaagaag
     3301 cagagctgac gattcttatt cgggaggata aagcccatga agcctgttcg acgttttttg
     3361 tttcttctcg tgtcagtcgt tctgctggct gtctccgcag aggctgcccc tttcaggccc
     3421 tccgagcctt atttgagaac gtcttccctc aggccgggta tgaaggggta cgctctgaca
     3481 gtcatctcag gacgggagat tctccggttt cctgtagaaa tcgtcagcat cattcccaga
     3541 aaaggatctc ccaaaaacct cgttatggtg cgggcttccg gttccgtgat atccaagacc
     3601 ggaggaatag cggccggaat gagcggcagc cctgtctata taaacggcaa actcataggc
     3661 gccgtcggct acggctggaa cttcagcgat cacaacctcg gccttgtgac tccctatgag
     3721 gatatggctt ctgtgtggga ctggccggaa aagatagtca ctctgccggg gcttcctcca
     3781 tccgggattg aaccccaaga tgaggaagac gatgacaaaa aaaagagctc tccccggaac
     3841 gaaactccag agaagagcgg cagcgggaat acggatgagc ccgttctaaa aggagaactt
     3901 tcgcctcttt tcattgatgg aatcagcgga aggacggcag cggaaatcac ggctctcctt
     3961 ggaaaggaac ggttcatacc gggcgggagc ttttccgaag acataccggt cgaaatgaat
     4021 gcaaggcttg ttcccggaga agcgataacc gttctccttg cctggggtga tgtaaccata
     4081 ggatccaccg gcaccctcac ggcggtcgca ccggatggcc ggttcatcgg cttcgctcac
     4141 cccttcctcg ggaggggagc cgtaaactac cccgttgcca gggcgta
//
LOCUS       DAOTHD010000055         4128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_25633_length_4128_cov_3.232752, whole genome shotgun sequence.
ACCESSION   DAOTHD010000055 DAOTHD010000000
VERSION     DAOTHD010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25633_length_4128_cov_3.232752"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="P5219_01640"
     CDS             complement(<1..573)
                     /locus_tag="P5219_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HRX25487.1"
                     /translation="MYCFQCQETARNQGCTVKGVCGKPEGTANLQDLLIYVCKGISVY
                     GEKLAKQGTVDREAGRFICKALFVTITNAAWDDDSIIEWIRKGLKVKDAIAQKAGASG
                     ALPDCAVWTADSKEQIMAKAMSDEVRITAAQNEDVRSLRELVIIGSKGVAAYAEHAEV
                     LGFEKDEIYASIMEALASTTKDLSVDDMVGM"
     gene            complement(659..1420)
                     /locus_tag="P5219_01645"
     CDS             complement(659..1420)
                     /locus_tag="P5219_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRX25488.1"
                     /translation="MTKVLRKIITIDEDKCDGCGICADACHEGAIQIIGGKAKLVSET
                     YCDGLGDCIGECPRGAITFEEREADPYDEEAVKKHMAAKAAAGPLPCGCPGSMARDLR
                     KEPEPCAVPVPSAAPAALKSSLANWPVQIRLVPETAPYLRKAELVIAADCTPFAFADF
                     HRTFLAGQNKVCLVGCPKLDDAQAYVEKLTRLIAYNEITSVTVVRMEVPCCGGMTRIL
                     EAACGAAEHNVSLKIVTIGVGGEITDKETIRFSRK"
     gene            complement(1417..1827)
                     /locus_tag="P5219_01650"
     CDS             complement(1417..1827)
                     /locus_tag="P5219_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRX25489.1"
                     /translation="MSGVVAVSEAVSLAFHGMGLLVSGSRMSAKEMAEAVNVSEAHLA
                     KVFQKLAKDGLVTSVRGPGGGFQLARDPAEIALFDIYTVIEGVPRDDYCLLHSERCPF
                     GSCIFGSLLEKMTDEFVEYLKNTTLADLQRGETL"
     gene            complement(1948..2274)
                     /locus_tag="P5219_01655"
     CDS             complement(1948..2274)
                     /locus_tag="P5219_01655"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRX25490.1"
                     /translation="MKLVDMNRLEKDQILPGLRARFVHSENMTVAYWNMDAGADFPLH
                     DHTHEQVVNVVSGRVEVLVRGEKTVLDEGCVLVLAPGEPHSVHAVMDSFVIDVFHPIR
                     QDYKRE"
     gene            complement(2343..3794)
                     /locus_tag="P5219_01660"
                     /pseudo
     CDS             complement(2343..3794)
                     /locus_tag="P5219_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
     gene            complement(3797..>4128)
                     /locus_tag="P5219_01665"
     CDS             complement(3797..>4128)
                     /locus_tag="P5219_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HRX25491.1"
                     /translation="SVDSIVNPNSALASVILRYARYPTGSGVLSIIEKIDAEMLEVAI
                     PERSPAVGRTIMSLGLPKGILVALIVRRGEVFVPVGATVLQAGDKVIVFATSDLMPEA
                     VEYLGVN"
BASE COUNT          862 a         1208 c         1235 g          823 t
ORIGIN      
        1 catgccgacc atatcgtcga cggagaggtc tttcgtggtc gaggcaaggg cttccatgat
       61 ggatgcgtaa atctcgtcct tctcgaagcc gaggacctcg gcgtgctccg cgtaggcggc
      121 gacgcctttg ctgccgatga tgacgagctc tctcagggag cggacgtcct cgttctgcgc
      181 tgcggtgatg cggacttcgt cggacatggc ctttgccatg atctgttctt tgctgtccgc
      241 cgtccatacg gcacagtcgg ggagcgcacc ggatgctcct gctttctgcg caatcgcgtc
      301 cttgaccttc agccccttgc ggatccactc gatgatggaa tcgtcatccc aggcagcgtt
      361 ggtgatggtg acaaagagcg ccttgcagat gaatcgtcct gcttccctgt ccaccgtccc
      421 ctgcttcgcc agcttttcgc cgtacaccga gatgcctttg cagacataaa taagaagatc
      481 ctgaagattg gcggttccct ccggttttcc gcagacgccc tttaccgtgc atccctggtt
      541 cctcgctgtt tcctggcact gaaaacaata cattgctttc cctccttctt gatttttaag
      601 ccgatggaaa tgaacaggcc ttccgctcac ttcccctatc ctgaaactgc ttctcctgct
      661 attttcggga gaaccttatg gtctccttgt ccgtaatctc tccgccaacg ccgatggtga
      721 ctattttgag cgagacgtta tgttcggccg caccgcaggc agcctcaaga atcctggtca
      781 tgccgccgca gcaggggact tccatgcgga ccacggtgac ggaggtgatt tcgttgtagg
      841 caatcagcct ggtgagcttc tccacgtagg cctgggcgtc gtcgagcttg gggcagccca
      901 cgaggcagac cttgttctgg ccggcgagga atgtgcggtg gaaatcggcg aaggcaaagg
      961 gggtacagtc tgcggcgatc accagttccg ccttccggag atagggggcg gtctcgggga
     1021 ccagccggat ctggacaggc cagttggcga gagaggattt caatgccgcc ggcgctgccg
     1081 acggtaccgg caccgcacag ggctcaggtt ctttcctgag atcccgggcc atggatccgg
     1141 ggcagccgca ggggagcggc ccggccgcgg ccttcgccgc catgtgcttc ttcaccgctt
     1201 cctcgtcgta ggggtcagcc tctctttcct cgaaggtgat ggctccccgg gggcattcgc
     1261 cgatgcagtc gccgagaccg tcgcagtagg tctcgctgac aagcttcgcc tttccgccga
     1321 tgatctgtat ggctccctcg tggcaggcgt cggcacagat gccgcagccg tcgcacttgt
     1381 cctcgtcgat ggtgatgatt ttccgcagta ccttggtcat agggtttctc ctctctgtaa
     1441 atccgcaagg gtggtatttt tcagatactc aacaaactcg tccgtcatct tttccagaag
     1501 ggagccgaaa atgcagctgc cgaaggggca tctttcgctg tggagcaggc agtagtcatc
     1561 ccgtggaacg ccttcgataa cggtgtagat gtcaaaaagg gcaatctccg cggggtcccg
     1621 ggcaagctga aacccgcctc cgggtccccg gacggaagtc accagcccgt cctttgccaa
     1681 tttctggaaa accttggcca ggtgggcctc ggagacattc accgcttccg ccatttcctt
     1741 cgcgctcatc cgacttccgg aaacgagaag acccattccg tgaaaggcca gggataccgc
     1801 ttccgacaca gcaaccactc cgctcataat taaggcccct ttctcaattc aggaattatg
     1861 gtactgtaat tccgaaatat gtcaaggggg cggaaccaaa aaagaataaa aaaggaaagc
     1921 gggctgaaag ccgctctcct gcaacgctca ctctcttttg tagtcctgcc ggatggggtg
     1981 gaacacgtcg atcacgaagc tgtccatcac ggcgtgaacc gagtggggct cacccggcgc
     2041 caggacgaga acgcaccctt cgtccagtac cgtcttctct cctctgacca ggacctccac
     2101 ccttcccgaa accacgttca ccacctgctc gtgggtgtgg tcgtggaggg gaaaatccgc
     2161 tcccgcatcc atgttccagt aggctaccgt catgttctcg gagtggacga acctggcccg
     2221 aaggccggga agaatctgat ccttttccag ccggttcatg tcgacgagtt tcatgaccat
     2281 cccccctcca tactgaattt tcggaattta ttatagcata gaggggaaag aagagaaaag
     2341 aactaccgtc tccaggtttc cgggacaaag agcaccacca cggtgtagag ctcaagcctt
     2401 cccatgagca tgagaagaga gagcacccac tttccggtct ggggaatgtc ggcatagttt
     2461 ctcatgggac ccacgccgcc gagaccgggg ccgatgttgc ccagggtggc ggcgacgctg
     2521 gacaaggagg tgaggatatc aagaccgagg gcggccatga acagagtccc ggcggtgaag
     2581 agagccatgt agaggatgaa gaaggaggtc accgaggcga tgatgtcctt tccggccact
     2641 ttgcccccta ccttcacctg cactatcgcc ctggggtgga ggatggagga gagttcggcc
     2701 tttacatgcc gcgcaaggac catcagccgg aggtttttta tccctccgcc ggtggaaccg
     2761 gcacatgccc cgacgaacat caggaggaga aggagaaact gggtgtagga aggccagagt
     2821 tcatagtccg ccgtcacata ccccgtggtg gtgatgatgc tcaccacctg gaaggcgccg
     2881 aaccggaggg cgtcggccac ggtgtcgtag gttccgtgga gcaggagcac tcccgccacc
     2941 gtcacggtgc agaaaaggac gacccccgtg tagaaccgga attcctcgtc cctgtggaaa
     3001 gcggagaacc gccccatgat aaaccggtag tggagggcga aattagcccc ggcgagaaac
     3061 atgaagaggg tgatcaccca gtcaaagtag gcggaattgt attgtccgac gctcttgttc
     3121 agcggggaga agcctcccgt ggccatggtg ccgaaggtat gggtgagggc ttcaaagagg
     3181 ttcatgcctc cgaacatgag aagcagggtt tccgcccccg acagaagcac gtagaccccc
     3241 cagagataga gggccgtctg ctgtatccgg ggagtcatct tctcggggat gggaccgggg
     3301 acttccgcct tgtagagctg catgccgccc acgccgatga aggggaggat cgccaggctg
     3361 aggacgatga tgcccatgcc gccaagccag tgggtgagat cccgccagaa aaggatgccc
     3421 ctggggttcg cctcgatgtc ggagatgacg gaggctcccg tggtggtgaa tccagacatg
     3481 gcctcgaaga aggcgtcggc aaaggtggga accgccccgt ggagatagta gggaagggca
     3541 cccacaacgg aggcgatgat ccacgaaagg gagaccacca cgaagccgtc cttgacgccc
     3601 agatcctcat agtcggcctt cctgccgacg aggtagagcc ctcccgacag gacgcccccc
     3661 atcattatgg aaaagagaaa gggcttcacg tcgccgctgc cgtcgtacag cgaccacgcc
     3721 aggggtcaga tcatggagag ggagactatg ccggacagca gcgagaggac cttcgcgacg
     3781 agacggaaat gcacggctag ttcactccga gatactcgac ggcttcgggc atgaggtcgg
     3841 aggtggcgaa tacgatgacc ttgtcccccg cctggagcac tgtggctcca acagggacga
     3901 agacttctcc ccgccggacg atcagggcca ccaggatccc tttcggcagg cccaggctca
     3961 tgatggttcg ccccactgcg ggactccgtt ccggaatggc cacctcgagc atctccgcat
     4021 cgatcttctc gatgatggaa agcacgccgg agccggtggg gtagcgggcg tagcgaagta
     4081 tcaccgacgc aagggcactg ttggggttca cgatggaatc aacggaca
//
LOCUS       DAOTHD010000056         4119 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_25694_length_4119_cov_2.875246, whole genome shotgun sequence.
ACCESSION   DAOTHD010000056 DAOTHD010000000
VERSION     DAOTHD010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25694_length_4119_cov_2.875246"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="P5219_01670"
     CDS             complement(<1..324)
                     /locus_tag="P5219_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HRX25492.1"
                     /translation="MASVRAICVSTERQQPKTEVERVLFLPGGIEGDSHRGVTEREVS
                     LLRAEDIRKAEEEAGFPFPPGSLAENLVAEGLPEELPVGARLAVGSSVLLEVVEKGKK
                     PGEPHS"
     gene            complement(459..1781)
                     /gene="codA"
                     /locus_tag="P5219_01675"
     CDS             complement(459..1781)
                     /gene="codA"
                     /locus_tag="P5219_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine deaminase"
                     /protein_id="HRX25493.1"
                     /translation="MDLIIRRARLAKGEIADIGISGGVFAAVEQRLECRADREIDAGG
                     RLTTPPFADPHLHLDAVLTVGSPRYNVSGTLLEGIAIWGQRRPGVTKDEILRNAREAV
                     LWEAANGVQFIRTHADTTDPSLVTVEALLELKDEMREIAEIQVVAFPQDGIYTIPGGE
                     RNLRRAVEMGCDCVGAIPHNELTREDGVRSVELAFDLAEKYGRLVDIHCDETGDDQSR
                     FVEVMAKCAIASGMGTRTTASHTTAMHNYNNDYALKLMGIFRRARMNFITLPFDNSVL
                     QNRTDGYPRRRGHTRVDELDAAGVNVCIGHDSIMDPWYPLGKGSMLAAANLLLHTAHM
                     SGYDQIFRLFDMITVNSARTMNVENRYGIEPGKPANLLVLDAPDQFEAIRLQSECLWS
                     VRNGSVIAETVPARRTLSWSGRKRHVDFSVPGVAGEEEALPVLLPRMA"
     gene            2110..3102
                     /locus_tag="P5219_01680"
     CDS             2110..3102
                     /locus_tag="P5219_01680"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HRX25494.1"
                     /translation="MRKSVLALLMVFVLLLAGTASAEVKDYKDFKLAAVFQTAIEEPW
                     DGAIHQACILLQKELGFTYEFTEKVGAADFERVLREYAERGFDLIVGDAFLAGEEPSR
                     RVAKDYPEVAFAFGSEFTFQEPNYSVFDNWIHEPSYLCGIIAGRITKTNTLGIVAALP
                     IAEVNRLVHAFKEGALSVNPKVKVKVAYIGSWFDPPKAKEATLAQIEAGADLIFAERF
                     GVFEAAKEKGVLAFGNMMDQHDLAPEVVITGPVWNMGPTVRYCIDEVRSKKWKAGDLR
                     EWSMMARGGAYLAPFHEFQQKLPPEVLKEVRELEAKIMNGQFTVPIIETELKTD"
     gene            3187..>4119
                     /locus_tag="P5219_01685"
     CDS             3187..>4119
                     /locus_tag="P5219_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRX25495.1"
                     /translation="MPADRHAMSAGTEEAPASGHTAPPCVVEMRSITKTFLDVTANSN
                     VTFSLREGEICALLGENGAGKTTLMNILFGYYAADSGEIRIGGEKVSFSSPKDAIACR
                     IGMVHQHFTLVPSQTVLENVVVGSGGGKFFLDLSGARKKLLALQERFGLSVDPDAPVW
                     TLPIGGQQKVEILKALYRDARILILDEPTAVLAPLETRELFATLRTLAAGGCSIIFIS
                     HKLYEVMEIADRVVVLTKGVLTAERKVSETSERELANLMVGRDLAEKKRPPRGTPGSP
                     LLEIRGLTVKNDRNLEAVNDLSLDIRSGEILGMAG"
BASE COUNT          815 a         1301 c         1227 g          776 t
ORIGIN      
        1 ggaatggggt tccccgggtt ttttcccctt ttccacaacc tcaaggagga cggaactccc
       61 caccgccagg cgggcaccca cgggtagttc ttcgggaagc ccttccgcca ctaggttctc
      121 ggccagggat cccggaggga agggaaaacc ggcctcttct tcagccttcc ggatatcctc
      181 ggcacgaaga agactcactt cccgctccgt cacgcccctg tgggaatctc cctcaatccc
      241 gccgggaagg aaaaggaccc gctccacctc tgttttcggc tgctgccgtt ccgtgctcac
      301 gcaaatggcc ctcactgaag ccatgaatat cacgcccccg cctgttcgtt ataaagaaaa
      361 gaaccagttc ataatacgat agacgataaa aaaaggcgag gccccggggc cccgcacttc
      421 actttcagca ggcatcggcg gcgtgccgcc ggtgttcgtc aggccatcct cggcagaaga
      481 acgggaagag cttcctcttc tccggccacc ccggggacgg agaagtccac atggcgcttc
      541 ctcccgctcc aggagagagt ccgccgggcc ggcaccgttt cggcgatgac ggagccgttc
      601 cgcaccgacc agaggcactc gctctggagc cggatcgcct cgaactggtc cggagcgtcc
      661 aggaccagaa ggttggcagg ctttcccggt tcgatgccgt agcggttttc cacgttcatg
      721 gtccgggctg aattgacggt gatcatgtcg aagaggcgga atatctggtc gtagccgctc
      781 atgtgggccg tgtgaagcag caggttggct gcggcgagca tggatccctt ccccagaggg
      841 taccaggggt ccatgatgga atcatggccg atgcagacgt tcacgcccgc cgcgtccagc
      901 tcgtccaccc gggtgtggcc cctgcggcgg ggatagccgt cggtccggtt ctggagcacc
      961 gagttgtcga agggaagggt gatgaagttc atcctcgccc gccggaaaat gcccatgagc
     1021 ttcagggcgt agtcattgtt gtaattgtgc atggccgtgg tatggctcgc cgtcgttcgg
     1081 gtccccatgc cggaggcgat ggcgcacttg gccatgacct cgacaaaccg ggactggtcg
     1141 tcccccgtct cgtcgcagtg gatgtccacg agccggccgt acttctccgc cagatcgaag
     1201 gccagttcca cggaccggac gccgtcctcc cgggtcagct cgttgtgggg gatggcgccg
     1261 acgcagtcgc accccatctc cacggcgcgg cgaagattcc gttcgccgcc ggggatggtg
     1321 tagatgccgt cctggggaaa agccaccacc tggatttcgg caatctcccg catctcgtcc
     1381 ttgagttcca gcagggcttc cacggtaacc agtgacgggt cggtggtgtc ggcgtgggtc
     1441 cggatgaact gcacgccgtt ggccgcctcc cagaggacgg cttccctggc gttcctgagg
     1501 atctcatcct tcgtgacgcc cggacggcgc tggccccaga tggcgatgcc ttcgaggagg
     1561 gtgcccgaaa cgttgtaccg gggtgacccc acggtgagga ccgcgtcgag gtgaagatgg
     1621 ggatccgcga aggggggtgt cgtcagcctg ccgcccgcgt cgatctcccg gtccgcgcgg
     1681 cactcaagcc gctgttccac cgcggcgaag acgccgccgc tgatgccgat gtccgcgatt
     1741 tctccctttg caagccgtgc ccggcgaata atcaggtcca tggtcatttc ccctctctca
     1801 tttctatgct accggttgcg cattttaaca cgaaggctgt ccggtgtcca tggctgacaa
     1861 aatttcggag aattctcgtt acatctcacc attgacatgg gacgaaggca accgtttaat
     1921 gaagtgaata agcatttcgt cggtacattt ctgataaaat aagatacaac ccttatcctg
     1981 caccggaaca tacggggagg tgagcaggaa aagccgatcc accgggaaag ggtgcgtccg
     2041 aacggcagtt ggggcatgcg tccgcgatcc atggcggccg ctggtaccga gaccagggag
     2101 gtgtatcttt tgaggaaaag cgtattggcg ttgttgatgg tattcgttct gctcctcgcc
     2161 ggaaccgcgt cggcggaggt gaaggactac aaggatttca agctggccgc cgttttccag
     2221 acggccatcg aggaaccctg ggatggggcg attcaccagg cctgcatcct gcttcagaag
     2281 gaactcggct tcacctatga atttaccgaa aaggtcggag ctgccgactt tgagcgggtt
     2341 ctcagggaat acgccgagag gggattcgat ctcatcgtgg gtgatgcctt cctcgccggg
     2401 gaggaacctt cccgccgggt cgcgaaagat tacccggaag tcgccttcgc cttcggatcc
     2461 gagttcacgt tccaggaacc caactactcc gtgttcgaca actggattca tgaaccctcc
     2521 tatctctgcg gcatcatcgc cggccgcatt acaaaaacca acaccctcgg catcgtcgcc
     2581 gcccttccca tcgcggaggt caaccgcctc gtccatgcct tcaaggaagg cgccctttcg
     2641 gtcaacccca aggtcaaggt gaaggttgcc tacatcggca gctggttcga tccccccaag
     2701 gccaaggaag cgactctcgc ccagatcgag gccggagccg acctcatttt cgccgaacgg
     2761 ttcggcgtct ttgaagcggc caaggaaaag ggcgtcctcg ccttcggaaa catgatggac
     2821 cagcacgacc tcgcccctga agtcgtcatc accggaccgg tatggaacat ggggcccacc
     2881 gtacgctact gcattgacga agtgaggagc aagaagtgga aagccggaga cctgagagag
     2941 tggtccatga tggcccgggg cggcgcatat ctcgccccct tccacgagtt ccagcagaaa
     3001 cttcctcccg aagtgctcaa ggaagtccgg gaactggaag cgaagatcat gaacggccag
     3061 ttcaccgtgc ccatcatcga gaccgagctg aagacggact gacatccgct cccggggcgg
     3121 gccttcctgc ccgccccttt cgtccttatc cttttttatc cgcagctacc gaaagaggtg
     3181 gataccatgc ccgctgacag gcacgccatg agcgccggga cggaagaagc cccggcctcc
     3241 gggcacacag cccctccctg cgtcgtggaa atgcgctcca tcacgaaaac gttcctcgac
     3301 gtcacggcga acagcaatgt gaccttttcc ctcagggaag gggaaatctg cgcccttctc
     3361 ggcgaaaacg gcgccggaaa gaccacgctc atgaacattc tcttcggcta ttacgccgcc
     3421 gacagcggag aaatccgcat cgggggcgag aaggtctcct tttcgtcccc caaggacgcc
     3481 atcgcgtgcc gcatcggcat ggtccaccag cacttcaccc tggtgccgtc ccagaccgtg
     3541 cttgaaaacg tggtggtggg aagcggcgga ggaaaattct tcctcgatct ctccggggcc
     3601 cggaagaagc tcctcgctct ccaggagcgc ttcgggctct ccgtggaccc cgacgccccg
     3661 gtgtggaccc tccccatcgg cggacagcag aaggtggaaa tactgaaggc cctctacagg
     3721 gacgcccgca tcctgatcct ggacgagccc acggcggtgc tcgcccccct tgagaccagg
     3781 gagctgttcg cgaccctccg caccctcgcc gccgggggct gctccatcat cttcatctcc
     3841 cacaagctct acgaagtcat ggaaatagcc gaccgggtgg tggtcctcac aaagggggtc
     3901 ctcaccgccg agagaaaggt ctccgagacc agcgaacggg agctggccaa cctcatggtg
     3961 ggccgggatc tcgcggagaa aaagcgtccc cccaggggaa ccccgggctc ccccctcctg
     4021 gaaattaggg gactgacggt aaaaaacgac aggaacctcg aagccgtgaa cgatctctcc
     4081 ctggacatcc gttccgggga gatcctgggc atggcagga
//
LOCUS       DAOTHD010000057         4113 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_25744_length_4113_cov_3.803598, whole genome shotgun sequence.
ACCESSION   DAOTHD010000057 DAOTHD010000000
VERSION     DAOTHD010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25744_length_4113_cov_3.803598"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="P5219_01690"
     CDS             complement(<1..1156)
                     /locus_tag="P5219_01690"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRX25496.1"
                     /translation="MTAKRAATAILTLILIFAAGPASARGGVVLALSGGGTRGLAHIG
                     VLEVLEEEGIPIAGIVGTSMGSIIGGLAASGYSSAELREVIAGLDLTNLLSEKTNPIF
                     VPLSQDSVSPRSKVPWVAISSSGDVVGPLGGMSGVKLLERFAQLASRSQIVRFDELAI
                     PFAAVATDLETGEKVVLRTGSLATAMRASMAIPALFDPWPVGGKLLVDGGLVSNLPVE
                     TAKDLFPGYPVIAVDVSGTLKKRKDIRSLVDVIDQSLTIFTRRNVENEIMKADLVISP
                     SVAGVPIFDLTHASRLIESGRQATMDRMAAIRNLAENAPPVAQRESLCIAATVASIRV
                     EGATADLERRIKNRYRYWIGKPLSNINMVRASREIGDQEDVLAVDYRFEEA"
     gene            1452..2654
                     /locus_tag="P5219_01695"
     CDS             1452..2654
                     /locus_tag="P5219_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HRX25497.1"
                     /translation="MVSGKKVPLIWQIGIGFVLGIAAGILFGDKVKYVEPIGQIFLAL
                     LKMLIVPLVFSSLVVGAASIGDPRDLGRIGIKTIVLYLVTTAVAIVIGLVLGNVIQPG
                     VGLALSQEASAFKAPEAPNVMKVILGLFPSNPIKAAADGVMLQIIVFALFLGISAVLA
                     GEKGKPVISFFDSLAEAMYKLTGIVMKTAPYGVFALIAVTVSKYGLSVLAPFAKVIGA
                     VYLGCAIHALLVYSGLIMTFAKKSPLWFFKGVREPALTAFVTRSSSATLPITMRTAQE
                     NLGVSEKVSSFVLPLGATINMDGTALYQGICALFVAQAYGLPLTMGAQMNIVLTATLA
                     SIGTAGVPGAGLIMLTLVLTAVGLPLEGAALVAGIDAVLDMARTCINVVGDSCVTAVV
                     ASTEGEKL"
     gene            complement(2730..3221)
                     /locus_tag="P5219_01700"
     CDS             complement(2730..3221)
                     /locus_tag="P5219_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HRX25498.1"
                     /translation="MRMGTEKLAVLVNTFGSSLMTVSKEMGVEVSLVKSEIAQGVNVT
                     GSCVAALVGIVGGGVQGTAVIMLDREGFNATVSAMSGGMITPDTEDAVSMSVIGELAN
                     MVSGRALVQAALEGTEVTPPQLISGAGIKTIPSQSPGVRSFTLPFRLPVGGTLFLVLS
                     FHV"
     gene            complement(3235..3687)
                     /locus_tag="P5219_01705"
     CDS             complement(3235..3687)
                     /locus_tag="P5219_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HRX25499.1"
                     /translation="MILGIDPGRDKCGWVFAAPGGELIASGIFPTERAADFLRAAVSG
                     EGRVIASFALENPGPFPESFSINECLVGNGTGKELFLSLAKSLSLGVKLIPEKGTTLL
                     ARKLYWKHHPPRGFRKLLPESMRVPPRDVDDFAALAIVLKCIESQSAQ"
     gene            complement(3684..>4113)
                     /locus_tag="P5219_01710"
     CDS             complement(3684..>4113)
                     /locus_tag="P5219_01710"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRX25500.1"
                     /translation="IIQKRAESGVTSVGKGFDLKAAIRCLQRGEALAILMDQDVRDKG
                     VVVPFLGLPASTPYGPAKIARKLGSTILPAFMVRRGTSMVHDFHILPSPWKKGYPEDD
                     ESMETAMTLCNDAISRFIREYPEQWMWLYPRWASTESRIR"
BASE COUNT          861 a         1205 c         1122 g          925 t
ORIGIN      
        1 cggcttcctc aaacctgtaa tccacagcga gcacatcctc ctggtcgccg atttccctgc
       61 tggcccgcac catgttgatg ttgctgaggg gctttcctat ccagtacctg tagcggttct
      121 ttattctccg ctcgaggtcg gcggtcgctc cctcgaccct gatggaggcg actgtggcgg
      181 cgatgcagag ggattccctc tgggcaactg gaggagcgtt ctccgccagg ttcctgatgg
      241 cggccatgcg gtccatggtg gcctgcctgc ctgattcgat gaggcgggac gcatgagtga
      301 ggtcaaagat tggaacaccg gccaccgaag gcgagatgac gagatcggcc ttcatgatct
      361 cgttttccac attcctgcgg gtaaaaatgg tgagagactg gtcgatgacg tcaacgagag
      421 agcggatatc cttccttttc ttgagggttc cggaaacatc cactgctatg accgggtaac
      481 cggggaaaag gtctttcgcc gtttccacgg gaagatttga gacgaggcca ccgtccacca
      541 gaagctttcc gcctacgggc caggggtcga aaagggccgg tatggccatg gaagcccgca
      601 tggcggtggc aaggctgcct gtccggagca cgactttttc cccggtttcg aggtctgtgg
      661 ccactgccgc gaagggaatg gccagttcgt cgaaccggac aatctggctc cgcgacgcaa
      721 gctgggcaaa acgttcgagg agcttgacgc ctgacatgcc gccgaggggg ccgactacgt
      781 cacccgacga gcttatggca acccagggaa ccttgctcct gggagaaacg ctgtcctgac
      841 tcagggggac aaaaatggga ttggtctttt ccgaaaggag gttcgtgaga tcaagtccgg
      901 ctatgacctc cctcagttcc gccgagctgt acccgctggc cgccagtccg ccgatgatgg
      961 atcccatgct ggtgcctacg atgcccgcaa tggggatgcc ttcttcctcc agaacctcga
     1021 gaacccctat gtgggccagc ccccgtgttc ctccgccgga aagggcgagg acaactcccc
     1081 cccgcgcgga ggcaggtccc gcggcaaaaa tgaggatgag tgttagaata gcggttgcag
     1141 ctcgttttgc ggtcatggca atacctcccg gggatgtacg gtaaaaaaag tacacacctg
     1201 cttggattat cctaacagaa ttcgtttttc ttcaccactt ttctcctttc tttttattat
     1261 cccccctgaa gagcaaaaaa agttgaaacg aaggaatacg gggatagatc accggaggta
     1321 tggcaaagga aagggaaata gagtatgctg tatttggaaa ttacggcctc ttgtttcaca
     1381 aaggagggat gagaagaaga cattattttt cgataatcgt tttttcaatc catttcttga
     1441 aggaggcggt agtggtgtca ggcaagaaag tacctctgat ttggcagata gggatcggat
     1501 ttgttctcgg tattgccgcg ggaattcttt tcggagacaa agtgaagtat gtggaaccca
     1561 tcgggcagat atttcttgcg cttttgaaga tgctcatcgt tcctctcgta ttttccagcc
     1621 ttgtggtggg ggcggcctcc atcggcgatc cccgtgacct cggaagaatc ggcatcaaga
     1681 ccattgtcct ctatcttgtg accaccgcgg tggccatcgt tatcggcctt gttctcggca
     1741 acgtgatcca gcccggtgtg ggactcgctc tttcccagga ggcctcagct ttcaaggccc
     1801 ccgaagcgcc gaacgtcatg aaagtcatcc tgggcctttt cccctccaac cccatcaagg
     1861 ccgccgctga cggcgtaatg ctccagatca tcgtcttcgc cctcttcctg ggaatttccg
     1921 ccgtgcttgc cggagaaaag ggcaagcccg tcatttcttt ctttgattcc ctggcggagg
     1981 caatgtacaa gctgacgggc attgtgatga aaacagcccc ctacggcgtg ttcgccctca
     2041 ttgcggtgac cgtctccaag tacggccttt ccgtgctcgc ccccttcgcc aaagtcatcg
     2101 gggcagttta cctcggatgt gccatccacg ccctgctggt ttattcaggc ctgatcatga
     2161 cgttcgccaa gaagtctccc ctgtggtttt tcaagggagt ccgtgaaccg gccctcacgg
     2221 ccttcgtgac ccgttcaagc tcggcgaccc tccccatcac catgaggacc gcccaggaaa
     2281 acctcggcgt ctccgagaaa gtatcctcct ttgtccttcc cctcggcgcc acgatcaaca
     2341 tggacggaac ggcgctctac cagggcatct gcgccctgtt cgtcgcccag gcctatggtc
     2401 tgcccctgac catgggagcc cagatgaaca tcgtgctcac ggcaaccctc gcttccatag
     2461 gcacggcggg agttcccggc gcggggctca tcatgctgac cctcgtgctg accgccgtcg
     2521 gacttcctct tgagggcgcc gcccttgtgg caggaatcga tgctgttctc gacatggcca
     2581 ggacgtgcat caacgtcgtg ggcgactcct gcgtcaccgc agtcgtcgcc tccaccgagg
     2641 gagaaaagct gtaaggaaga tttctccttc attacggaaa aaacagaggc gcccgggaaa
     2701 ccgggcgcct cttcggttcg gtgaaaggtt cagacatgga aggagagaac aagaaagagc
     2761 gttcccccga cgggaagcct gaagggcagg gtgaaactcc tgacccccgg ggactggctc
     2821 gggatcgtct tgattcccgc cccggaaatc agctggggag gggttacttc cgttccctcc
     2881 agggcagcct gcacaagggc cctcccgctg accatgttcg caagttcgcc aatgacgctc
     2941 atggagaccg cgtcctccgt gtccggggtg atcattcctc cggacatggc tgaaacagtc
     3001 gcattgaaac cttccctgtc gagcatgatg acagccgtcc cctgcactcc gccgccgaca
     3061 attcccacaa gggcggcgac gcagcttccg gtcacgttga ctccctgggc aatttccgac
     3121 ttcacaagag aaacttcgac tcccatctct ttactgacag tcatcagaga agacccgaat
     3181 gtgtttacaa gtaccgccag cttctccgtt cccattctca ttcacccttc ctgttcattg
     3241 agcgctttgc gattcgatac acttcaggac gatcgccagg gcggcaaaat catccacgtc
     3301 cctcggagga acacgcatgc tttccggaag cagtttacgg aagccccggg gaggatggtg
     3361 tttccagtac agtttcctcg ccagcagagt cgttcctttt tcaggtatta actttactcc
     3421 aagggacaag gattttgcaa gcgaaagaaa caattctttt ccggtaccgt ttcctacaag
     3481 gcattcattg atggaaaaag attccggaaa aggccctgga ttttcaagag caaaagatgc
     3541 aattaccctc ccctctccag aaacagcggc ccgtaaaaag tcggcggctc tctctgtggg
     3601 aaatatcccc gaggcaatca gctctccccc gggagcggca aaaacccatc cgcacttgtc
     3661 ccttccaggg tcaatgccga gaatcatcgt atccggctct ccgtcgaagc ccagcgcgga
     3721 tagagccaca tccactgctc cggatattcc cttatgaacc ggctgatcgc gtcattgcag
     3781 agagtcatgg ctgtttccat gctttcgtca tcctcgggat atcctttttt ccagggggag
     3841 ggaagtatat gaaaatcatg caccatggac gtgcccctgc ggaccataaa cgcgggaaga
     3901 atggtgctac ccagtttccg ggcgattttc gcagggccat agggagtgct cgcgggaaga
     3961 cccaggaacg gaaccaccac tcccttgtcc ctgacatcct ggtccatgag gatggccagg
     4021 gcttcccctc tctgaaggca tcgtatggcg gccttgaggt cgaagccctt gccgacggaa
     4081 gtaacaccgc tttcggctct tttctgtatg atg
//
LOCUS       DAOTHD010000058         4081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_26007_length_4081_cov_3.383010, whole genome shotgun sequence.
ACCESSION   DAOTHD010000058 DAOTHD010000000
VERSION     DAOTHD010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26007_length_4081_cov_3.383010"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="P5219_01715"
     CDS             complement(<1..698)
                     /locus_tag="P5219_01715"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRX25501.1"
                     /translation="MNEGIRLNRYLAMCGLGARRKVEEIIASGRVKVDGKVVLMPAYR
                     VSEENTVSVDGSLVQPESKVYVVMNKPAGWVCSVSDPFDPVIADLLPEKYRSFRLFPV
                     GRLDRETEGLLILTNDGAFAQEVLHPSRNILREYHALLDREINGKAVKKWLSGIELDG
                     RLLKPSSVRILDREPAGRWVSVVLAEGVKREVRRMAQSLGFEVEVLVRKRIGKMELKE
                     LPAGKFVEMSRGEL"
     gene            complement(695..1330)
                     /gene="scpB"
                     /locus_tag="P5219_01720"
     CDS             complement(695..1330)
                     /gene="scpB"
                     /locus_tag="P5219_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRX25502.1"
                     /translation="MCSYTASEGISDTGRKIQAILFVAGSPVPAAELAEVLSLPPSVT
                     SKEISRLDDFLRRTGSGVLLQETAGGWRMVTAEDTADAVSLFRDTAQTQKIRLSRAAV
                     ETLAVIAYNQPVTRSEVEDIRGVRCERVIETLLSHGMIRIAGRKKGTGSPLLYRTTDL
                     FLETFGLISISELPTLEELQELAPPPEEKNRKGAAGETEEGHIGGEGDDLP"
     gene            complement(1308..2087)
                     /locus_tag="P5219_01725"
     CDS             complement(1308..2087)
                     /locus_tag="P5219_01725"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRX25503.1"
                     /translation="MVQNHDLSGFQAEVSGFSGPFDLLCYLVENRQLDAAGISVGQAV
                     RIYGAYLANTRRVSVAVISEFLVLAASLVLNKIRSLLPGKTAEQEDGPCGTEEESRDE
                     FDVEEKLSRYRPYRKASAFLWNKKEKQDLVFSRPSSGDEGQTWDLGDLYGLCSLWWDI
                     LEEKRGSGSSAKSAWMDREDNWEGMPVPLPDEEQIDMKISEIMGILKNSPAVSFSKLL
                     GNSRSVSVFVVTLLSLLEMSRMGRIRIVQEELFRDVLIYSI"
     gene            complement(2097..3092)
                     /gene="trpS"
                     /locus_tag="P5219_01730"
     CDS             complement(2097..3092)
                     /gene="trpS"
                     /locus_tag="P5219_01730"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRX25504.1"
                     /translation="MNQRVFSGMRPTGQLHLGHLAGALTNWIKLQDGYECFYSIVDWH
                     ALMSDYADSSKIESNCREVLIDWLSSGLDPEKCVIFVQSHVPQHAELHLALSMVTPLG
                     WLQRCPTYKEQIINIQNKDLSNYAFLGYPVLMAADILLYKAGIVPVGEDQSAHLEIAR
                     EMVRRFNGFFGEVLVEPQIMLTPTPKVPGTDGKKMSKSYGNSINLADSPEDVWNKLRT
                     MVTDPAREKRTDPGDPDKCPVWDIHKVFNNSDGEKAEIAEGCKTAGIGCVDCKKKLKV
                     HVERVMNPIREKRAYYEKNPDLLGDILREGAVKAGAVARKTMDEVVNSIGFVRRG"
     gene            complement(3189..3971)
                     /locus_tag="P5219_01735"
     CDS             complement(3189..3971)
                     /locus_tag="P5219_01735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRX25505.1"
                     /translation="MLVKDLLKSLDTMVPFSWAEEWDNSGLLAGDPESPVTGVAVCLD
                     PSPEAMEFAAEKGCSVLVSHHPLIFSPLKKLDVSTGTGKAVAFALKRDIAVISMHTNW
                     DSSPRGVNAAIAAALGLDSVSALVPSLSGAWGLGALGDVPSALTGRELGQRILSVLNL
                     SRLDLYGDPHRPVNRLALCGGSGGSLWSGAKAAGADAFFTSDLKYHERMDSLASGLIL
                     LAGDHGEVESFSLDAMARVVSEAFGFEPPIFRTMPAIPVIIG"
BASE COUNT          869 a         1184 c         1092 g          936 t
ORIGIN      
        1 cagagttctc cccgggacat ctcgacgaac tttcccgcag ggagttcctt cagctccatt
       61 ttaccaattc ttttccgaac tagcacttca acctcaaagc cgaggctctg ggccatccgg
      121 cgcacctccc gcttgacccc ctcggcaagg acgacggaga cccaccgtcc cgccggttcc
      181 cggtcaagga tgcgtacgct cgacggtttc agcaggcgtc cgtccagctc aattccggaa
      241 agccattttt ttacggcttt cccgttgatt tccctgtcga ggagggcatg gtattcccgg
      301 aggatattcc gggaggggtg cagcacctcc tgggcgaagg cgccgtcatt ggtaaggata
      361 agaaggcctt ccgtttccct gtcaagccgt cccaccggaa acaggcggaa tgagcgatat
      421 ttttcgggaa ggaggtccgc aatgacgggg tcgaaggggt cgctgacgga acagacccag
      481 cctgcaggct tgttcatgac gacgtacact ttgctttccg gctgaacgag gctgccgtca
      541 acggaaacag tgttctcttc gctcacgcgg taggccggca tgagaaccac tttcccgtcc
      601 acctttacac ggccggaagc gattatttcc tccacttttc ttctcgctcc cagcccgcac
      661 atggccaggt accggttcag tcgtatccct tcattcatgg caggtcatcc ccctctccgc
      721 cgatatggcc ttcctccgtc tcaccggccg ctcctttccg gtttttttct tcgggcggag
      781 gcgcaagttc ctgaagctct tcgagcgtcg ggagttccga aatagagatc agcccgaagg
      841 tctccaggaa aaggtccgta gtcctataga gaagaggaga gccggttcct ttcttccttc
      901 ccgcgattct gatcatcccg tgggaaagga gggtttcaat cacccgttcg catcgtaccc
      961 cgcggatgtc ctccacctcg ctcctggtca ccggctgatt gtaggctatc actgcaagag
     1021 tttcgaccgc cgccctgctc aggcggattt tctgtgtctg ggctgtatcg cgaaacaggg
     1081 agactgcatc ggccgtgtcc tctgccgtca ccattcgcca cccgccggct gtttcctgga
     1141 gaagaacgcc tgatccggtc cgcctgagga aatcatcaag ccgggatatt tctttggacg
     1201 tgacagaagg gggcagggag aggacttcag cgagttcagc agccggaaca ggagaaccgg
     1261 caacgaagag gatcgcctga attttccggc cggtgtcaga aatgccttca gatgctgtat
     1321 atgagcacat ccctgaacaa ctcctcctgt acgatgcgga ttcttcccat tctgctcatt
     1381 tcaagaaggg aaagaagcgt cacgacaaaa acgcttacac tcctgctgtt tccgagaagc
     1441 ttcgaaaacg agaccgcagg ggaatttttg agaattccca ttatctcgga aattttcata
     1501 tcaatctgtt cctcgtcagg aaggggaaca ggcatacctt cccagttatc ctctctgtcc
     1561 atccaggctg actttgccga agaaccggaa cctcgttttt cttcaagaat gtcccaccag
     1621 agactgcaca gcccatagag gtcgccgagg tcccatgttt gtccttcatc tccggaggaa
     1681 gggcgggaaa aaacgagatc ctgcttttct tttttattcc acagaaatgc tgatgccttc
     1741 ctgtatggcc tgtaccggga aagcttttcc tcaacatcga actcatcacg ggattcttct
     1801 tccgtgccgc agggaccatc ttcctgctca gcggtttttc ccgggagaag agacctgatt
     1861 ttgttcagca cgagagaggc agccaggacg agaaactcgg agatcacggc gacggatacc
     1921 cgcctcgtgt tggccaggta ggccccgtaa atcctgaccg cctggccgac ggaaatcccg
     1981 gccgcatcga gctgcctgtt ttccacaagg tagcacagca gatcaaacgg gccggaaaaa
     2041 ccgcttactt ccgcctgaaa accactcagg tcgtggtttt gaaccacctc atcgacctac
     2101 cctctccgga cgaatcctat ggagttcaca acctcgtcca tggttttccg ggcgacagcc
     2161 ccggccttta ccgcaccctc cctgaggatg tccccgagaa ggtcgggatt cttttcataa
     2221 taagcccttt tctcccgtat ggggttcatg acccgctcaa cgtggacctt cagcttcttt
     2281 ttgcagtcaa cgcaaccgat gcctgcggtt ttgcagccct ccgcaatctc ggctttttcg
     2341 ccgtcactgt tgttgaagac cttgtggata tcccagacgg ggcatttgtc cggatcgccg
     2401 gggtcggtgc gtttttcccg ggcagggtcg gtcaccatgg tccgcagttt gttccagaca
     2461 tcttccgggc tgtcggcaag gttgatcgaa tttccgtagg atttgctcat cttctttccg
     2521 tcggttccgg gtactttcgg cgttggggtc agcatgatct ggggttctac caggacttcg
     2581 ccgaaaaagc cgttgaatct ccgcaccatt tccctggcga tttccaggtg ggcgctctgg
     2641 tcctctccca cgggcacgat tcccgccttg tacagaagaa tgtccgccgc catgaggaca
     2701 gggtatccga gaaatgcata attactgagg tccttgttct gtatgttgat gatctgctcc
     2761 ttgtaggtcg ggcatctctg gagccagccg agaggagtga ccatggagag ggcgaggtga
     2821 agctccgcgt gctgggggac gtgggactgc acgaagatga cgcacttttc agggtcaagg
     2881 ccggaagaaa gccagtcgat cagaacctcc ctgcagttcg attctatttt tgagctgtcg
     2941 gcatagtccg acatgagggc atgccagtcc acaatgctgt aaaagcactc gtatccatcc
     3001 tggagcttga tccagtttgt aagggctccc gcaagatgtc ccaggtggag ctgtcctgtc
     3061 ggcctcattc cgctgaaaac acgctgattc atttctaacc acctcgaatg ggaaatgtcc
     3121 ggaaaaatcc gtcaaaaaac ctgagatatc atatcatttg aaatgaaatc atcataggag
     3181 gaacggtgtc agccaataat gaccgggatt gccggcatag tcctgaaaat aggcggctcg
     3241 aagccgaaag cttcggaaac aacccgggcc atggcatcaa gtgaaaaaga ctccacttct
     3301 ccgtgatctc ccgcgaggag aatgagtccc gaggcgaggg aatccatcct ttcgtggtac
     3361 tttaggtccg atgtgaagaa ggcatcggct ccggcggctt tcgctccgct ccagagactt
     3421 cctccggatc ctccgcacag tgcgagccta ttcacggggc ggtgagggtc gccgtagaga
     3481 tcgagtcggg agaggttcag cactgaaagg atcctttgcc ccagttcgcg ccccgtcaac
     3541 gctgagggta catctcccag ggctccgagc ccccatgccc cggagagcga gggaacaaga
     3601 gcactgacgg aatccagtcc caaggccgcg gcaatggcgg cgttgacgcc tctcggactg
     3661 gagtcccagt ttgtgtgcat ggagatcacg gcaatgtccc ttttcagcgc gaaggcgacg
     3721 gcttttccgg taccggttga aacatccagc ttcttcagcg gagaaaagat cagaggatga
     3781 tggctaacca ggaccgaaca gcctttttcg gcggcaaatt ccattgcttc cggactcgga
     3841 tcgaggcata cggctacccc tgtcaccgga gactccggat ctccggcgag cagtcccgaa
     3901 ttgtcccact cctcggccca gctgaaagga accatggtgt caagggattt cagaagatcc
     3961 ttcaccagca tcgggattcc tcctccaaaa taaaaaaaac cgtttccccg gaaggaaacg
     4021 gcgatttcgt ggtgggccta cctggactcg aaccaggaac cttccggtta tgagccggtg
     4081 g
//
LOCUS       DAOTHD010000059         4072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_26081_length_4072_cov_3.512323, whole genome shotgun sequence.
ACCESSION   DAOTHD010000059 DAOTHD010000000
VERSION     DAOTHD010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26081_length_4072_cov_3.512323"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="P5219_01740"
     CDS             complement(<1..379)
                     /locus_tag="P5219_01740"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRX25506.1"
                     /translation="MKAFCIDEPGKYSFRDIPVPVPRDGEVLLRIKKVGYCGSDLNTW
                     RGLNPLVKYPRIPGHEISGVVEQVTRGVPAGVKVGDIVTAMPYTSCGKCWSCLSGRPN
                     ACKNNETLGVQREGSLTEFIVLPW"
     gene            complement(429..1715)
                     /locus_tag="P5219_01745"
     CDS             complement(429..1715)
                     /locus_tag="P5219_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRX25507.1"
                     /translation="MIVGLTFALLLVLLFINAPVFAAILGSSIFYFVANDSLSWMMVV
                     QRTISGLESIPLLAVPFFVMAGVFMNYTGITSRMIRFAEVLTGHMPGGLAQTNVVLST
                     LMGGLSGSSLADAAMQSKILVPEMEKRGYGKAFSSAVTGASALITPIIPPGIALIIYG
                     FVGNVSIGRLFLAGVVPGVMTCLFMMVVVHFISKRRGYLPIYEKRAGIREVFRAFREA
                     SWALFLPVVIVGGIRFGVFTPTEAGSIAILYALVLGTIVYREMTLKDLKTGLIETVTT
                     TASIMLIIAAASSFAWILTWERVPQITANWVLTMVSSPGVFLLLINVFLLIVGMFIEG
                     NAAMLVLIPIFMPMVQALGINDVHFGMVFIFNMAVGSLTPPMGTVMFTTCSITGAKIG
                     DYIRESVPFYLVLLFCLILITYVPQISLFLPNLLFN"
     gene            complement(1712..2203)
                     /locus_tag="P5219_01750"
     CDS             complement(1712..2203)
                     /locus_tag="P5219_01750"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRX25508.1"
                     /translation="MKTIKKLLYHADDIVASAALIVLVGSTILGVFYRYALSDPLDWV
                     EEVSLLCVVWFVFIGGSSATKRNSHVGIDFVIALFPVPVQKAAFVFVQILCYAVLSFM
                     AWWGWMLGLQAQLKVTNMLRIPYTYIDLAVPVGCILMMIHLAAGTGRNWKTLFAGNKE
                     AAS"
     gene            complement(2346..3329)
                     /locus_tag="P5219_01755"
     CDS             complement(2346..3329)
                     /locus_tag="P5219_01755"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HRX25509.1"
                     /translation="MFGKMTRIALCFALVFSLFAGAQQVAAAEYEVKIAYENNPGEPT
                     DVGVQEWARILKEISGGKVEAVLYPSSQLGAKKDVLEMILMGSNVITIADAGFLMDYV
                     PDIGIMYAPYLTDTYDEYFTLVDSPWFKKQMDAVAGHGFEIVTAKWIYGDRHLLANKA
                     VKTPKDLAGLKIRTPNMKILSKTIEYMGGIPTPMPLGEVYPAIAQGVIDGAENPYPVL
                     WGAKLYEKAKFLTPTGHVKNVSMWLGSHKYFASLPPEIAGFIKDAAEKACLFVNENVK
                     KDDVKAIENFRNAGVEIVEPDMEAFRKAVFPIYEELWGKELYEEVKKNMTK"
     gene            complement(3462..>4072)
                     /locus_tag="P5219_01760"
     CDS             complement(3462..>4072)
                     /locus_tag="P5219_01760"
                     /inference="COORDINATES: protein motif:HMM:NF016203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HRX25510.1"
                     /translation="RAETPEAAEEIRAAYDNMVRYCTSVRQYSISPGNFAGGLSTIEE
                     KSCGAYAKSGSRPIRGVIRVSERPSGKGLWLMDSLPDPYFMQFGYSNPHDTEGLMDLL
                     SAGCQVLFFVTGRGSPVGAPLAPVVKITGNSGTFRKMEEDMDFDAGPILSGETGFDEA
                     ALELESLVERICRGDRSKSEILGHREYAIGCKFQDTGRPRCC"
BASE COUNT          849 a         1246 c         1179 g          798 t
ORIGIN      
        1 cccatggcag gacgatgaac tccgtcaggg acccctcccg ctgaacccca agagtctcgt
       61 tgtttttgca tgcgttgggc cgcccgctca ggcacgacca gcatttcccg cagctcgtgt
      121 agggcatggc ggtgacgatg tcccccacct tcacgccggc gggaacgccc cgtgtcacct
      181 gctccaccac accgctgatc tcgtggcccg gaatgcgggg atacttcacc agggggttca
      241 ggccccgcca ggtgttcagg tcgctgccgc agtaccccac tttctttatg cggagaagca
      301 cttctccgtc cctgggcacg gggacgggaa tgtctctgaa ggaatacttg cccggttcgt
      361 cgatgcagaa cgctttcata gtcttcatct cctcgatggt tgttacaccc ggaacggagg
      421 tccggaaatc agttgaacag cagattcggc aggaacagcg aaatctgggg cacgtaggtg
      481 atgaggatca ggcagaaaag gagcaccagg tagaagggca ccgattccct gatgtagtcc
      541 ccgatcttcg ctcccgtgat ggagcaggtg gtgaacatca ccgttcccat ggggggcgtg
      601 agggatccca ccgccatgtt gaaaatgaac accatgccga aatggacatc gtttatcccc
      661 agggcctgca ccatgggcat gaatatgggg atgagcacca gcatggcggc gttcccttcg
      721 atgaacatgc ccacgatcag cagaaagacg ttgatcagga gaagaaaaac tcccgggctg
      781 gacaccatgg tcagcaccca gttcgccgtg atctggggca ccctttccca ggtgaggatc
      841 cacgcgaagg acgatgccgc cgcgatgatg agcatgatgg atgccgtagt cgtcaccgtc
      901 tcgataaggc ccgtcttcag gtccttcagg gtcatctcac ggtagacgat ggtgccgagc
      961 accagggcat aaagaatggc gatggacccc gcctcggtgg gggtgaacac gccgaacctg
     1021 atccctccca cgatcaccac ggggaggaag agggcccacg acgcctcccg gaacgcccgg
     1081 aacacttccc ggattcccgc ccgcttttcg tagatgggca gatagccccg ccgcttcgaa
     1141 ataaagtgaa cgaccaccat catgaagagg caggtcatca ctccgggcac caccccggca
     1201 aggaacagcc gcccgatgga cacgttgccc acgaagccgt agatgatcag ggcgatgccc
     1261 ggcgggatga tgggggtaat gagggccgaa gctcccgtca ccgccgaaga gaaggccttg
     1321 ccgtagcccc gcttctccat ctccggcacc aggatcttgg actgcatggc cgcgtccgcc
     1381 agggaagaac cggaaagtcc ccccatgaga gtgctcagca ccacgttggt ctgggccaaa
     1441 ccgccgggca tgtggccggt cagcacctcg gcgaagcgga tcatcctgct ggtgatgccg
     1501 gtgtagttca tgaacactcc cgccatgacg aagaagggaa cggccaggag gggaatggac
     1561 tcaaggccgc tgatggtccg ctggaccacc atcatccacg agagggagtc attggcgacg
     1621 aaatagaaga tggagctgcc gagaatcgct gcaaacaccg gggcattgat gaaaaggagt
     1681 accagcagga gcgcgaaggt cagaccgacg atcatgatgc cgcctccttg ttaccggcga
     1741 aaagggtctt ccagttccgc cccgttcccg cagccaggtg gatcatcatg agaatgcacc
     1801 ccacgggaac ggccaggtcg atataggtgt agggaatgcg gagcatgttg gtcaccttga
     1861 gctgtgcctg gagccccagc atccatcccc accaggccat gaaggaaagc acggcatagc
     1921 acagaatctg cacgaagacg aaggccgcct tctgcaccgg aacggggaaa agcgctatga
     1981 caaagtcaat gcccacatgg ctgtttctct tggtggcgga gctgccgccg atgaacacga
     2041 accagaccac gcagaggagg ctgacctcct ccacccagtc cagggggtca ctcagggcat
     2101 agcggtaaaa taccccgagg atggtgctgc ccaccaggac gatgagcgcc gcggacgcca
     2161 cgatgtcatc cgcgtgatac agcagttttt ttatggtttt caccttggcc cgaacctcca
     2221 ggtcataaga ataaaaactt caaaggccgc gaaagcacac ttccggggaa tggtccgccg
     2281 cagcggccga ataaggatcc tgccgggggg gagagaatcc cctcccgccc ggcggatttc
     2341 cttttctact tcgtcatgtt ctttttgact tcctcgtaca gctccttgcc ccacagctct
     2401 tcgtaaatgg ggaacacggc cttcctgaag gcttccatgt cgggctcaac gatttccact
     2461 ccggcgttcc ggaagttctc gatggccttg acgtcatcct tcttcacgtt ctcgttcacg
     2521 aagaggcacg ccttttcggc ggcatccttg atgaaaccgg cgatctccgg gggaagggac
     2581 gcgaaatact tatggctgcc cagccacatg gacacgttct tcacgtgccc cgtgggggtc
     2641 aggaacttcg ccttctcgta cagcttggcg ccccacagaa cgggataggg gttctcggcg
     2701 ccgtcgatca ctccctgggc gatggcggga tacacttcgc cgaggggcat gggagtggga
     2761 atgcctccca tgtattcgat ggtcttggag aggatcttca tgttgggggt gcggatcttc
     2821 agcccggcaa gatccttggg ggtctttacg gccttgttgg ccagaaggtg ccggtcaccg
     2881 taaatccact tggcggtgac gatctcgaag ccgtgtcccg ccacggcgtc catctgcttc
     2941 ttgaaccagg gcgaatccac cagcgtgaaa tactcgtcat aggtgtcggt gaggtagggg
     3001 gcatacatga tcccgatgtc gggcacgtag tccatgagga acccggcgtc ggcgatggtg
     3061 atcacgttgc ttcccatgag gatcatctcg aggacatcct tcttcgcgcc gagctgggag
     3121 ctgggataca gaacggcctc gaccttgccg ccgctgatct ccttcaaaat ccgggcccat
     3181 tcctggacac ccacgtcggt gggttctccc gggttgttct cataggcgat cttgacctcg
     3241 tactcagccg ccgcaacctg ctgtgccccc gcgaaaaggc tgaaaaccag ggcgaagcac
     3301 agagcgattc gtgtcatttt tccgaacatg gcattccctc ctgaagtaaa tgatgtacat
     3361 tgcgtctctc gttccccgga agtgtgcatg cgccttgaaa ccttggttgt ggatccccgt
     3421 tttccttcat cacatccctt ccccggaatt tccggcattc ctcagcagca ccggggccgg
     3481 ccggtatcct gaaatttgca gcctatggca tattccctgt ggccgaggat ctcgctcttc
     3541 gagcgatctc cccggcagat tctttccaca aggctctcca gttcaagggc tgcttcatcg
     3601 aaacccgttt ctcccgaaag aatgggcccc gcatcgaaat ccatgtcctc ttccatcttc
     3661 cggaaggtac cgctgttccc ggtgatcttc accaccggcg ccaggggcgc ccccacgggg
     3721 cttccccgcc ccgtcacgaa gaaaagcacc tgacaccctg ccgaaaggag gtccatcaga
     3781 ccctcggtat catgggggtt gctgtagccg aactgcatga aatagggatc gggcaggctg
     3841 tccatcagcc agagcccttt cccggaaggc cgttctgaaa cccgtatcac gccccggatg
     3901 ggccgggagc ccgactttgc gtaagccccg cagctcttct cctctatggt ggaaagacct
     3961 ccggcgaagt ttcccgggga gatggaatac tggcggacgg acgtgcagta ccgcaccatg
     4021 ttgtcgtagg ccgcccggat ctcctccgcc gcttccgggg tctccgccct cc
//
LOCUS       DAOTHD010000060         4055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_26225_length_4055_cov_2.791500, whole genome shotgun sequence.
ACCESSION   DAOTHD010000060 DAOTHD010000000
VERSION     DAOTHD010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26225_length_4055_cov_2.791500"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>942
                     /gene="glgP"
                     /locus_tag="P5219_01765"
     CDS             <1..>942
                     /gene="glgP"
                     /locus_tag="P5219_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRX25511.1"
                     /translation="KLTWHLYGGDQIYRLCQEMVLGVGGLRMLRDLGYKNIETFHMNE
                     GHAGFLTLELMREQGYYDPEKIRDQVIFTTHTPVPAGHDFFRFDLVDKVISQEALSNL
                     KRMLPNADGVSMTELGIRFSRYVNAVSKKHAEVSRNMFNMPEIDWVTNGVHPGTWVSS
                     GMRRLFTTHIPGWERDPGGLVQAVKIPPEEIWTAHQASKMRLLAGVMEQTGRQLDPDV
                     LTIGFARRAAQYKRADLLLSNIRRLMEVGAGKIQLVFAGKAHPRDDGGKTVLQRLLTA
                     SQKVDEMIPIVFLENYDMEKGALITQGVDLWLNTPLRP"
     assembly_gap    943..952
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <953..1298
                     /locus_tag="P5219_01770"
     CDS             <953..1298
                     /locus_tag="P5219_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRX25512.1"
                     /translation="EASGTSGMKCALNGVMNFSVLDGWWIEGWLEDVTGWSVGPEPSE
                     SDMMHYDESLDAIDLYDKLEKKIIPTYYHNREKWIWMMQRTIALNGSFFNTHRVTREY
                     CEKAYNISFRGM"
     gene            1304..2767
                     /locus_tag="P5219_01775"
     CDS             1304..2767
                     /locus_tag="P5219_01775"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRX25513.1"
                     /translation="MTAVQGGRTPKILHVSPEAVPFSKVGGLGDVAGSLPAALRDSGT
                     DCRLLTPAWEGVLDRARELGFSLSRLSRKAEAVIRWKIHRGTVWKCSGEGLVAYLLEE
                     PSLFGKTVYPRELTPDSVTPFLFLSLAALDLPSATLWKPDIIHCHDWGTAPLPAALKW
                     HIHFGSSGDVCKTVFTIHNLAHQGLLSLESLGDWGIRNEAARVEGMEYFGMANLMKGA
                     LVSCDAITTVSPGYAEEILTEEGGEGLGGLLKSLSGKVSGILNGLDDRYWDPRTDPLL
                     PERYSPDNLSGKTACKKKLLERAGWKDDGRPLLVSVGRMVEQKGFSILLPALEELVGM
                     NCRLFLVGSGQTEYEEAADSAAARFPGSVFVFRGYDEPLAHLAYAGGDFFLMPSRFEP
                     CGLSQLISLRYGTVPIVRAAGGLRDTVFEHGTPGGNGFLFSGYTPEDLLEAVARAMEV
                     FRDKDSMQLLSLRGMNADFSWNRSAPLYKKLYESLLL"
     gene            2838..>4055
                     /gene="glgC"
                     /locus_tag="P5219_01780"
     CDS             2838..>4055
                     /gene="glgC"
                     /locus_tag="P5219_01780"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HRX25514.1"
                     /translation="MQVGKYGRTLGIVLAGGKGERLMPLTKYRAKPAVHFAAKYRIVD
                     FALSNLVNSGIFSIYVLVQFKSQSLNEHIERGWQFGGALRGRDFFITLVPAQMWRGEH
                     WFQGTADAVYQNLHLVTLYDADRVAIFAADHIYKMDVDQMLAYHIGKEADITVAANVV
                     PTSEASAFGCIDTDAAGRIVGFVEKPENPPEIPGNPGFTYVSMGNYIFEREILEESLI
                     EDAKLPTSHDFGRDIIPKLVGTSRIFAYDFSTNVLPGKEKSSFWREDKPYWKDVGTIR
                     AYWDAHMDLLKHDSEMTFYNPQWPIRTVSYADPPSYVCPVKGYTCNVQSTLSAEGSRV
                     LGSTVESSVLSRNCVILPGSSVEESIIGEGVVVGENCRLRRVIIDSHNIIPPNTVMGY
                     DEEHDRERYTVDPG"
BASE COUNT          883 a         1229 c         1164 g          769 t
ORIGIN      
        1 aaagctcacc tggcatcttt acggcggcga ccagatttat cgtctctgcc aggagatggt
       61 ccttggggtg ggaggactga ggatgctccg ggacctggga tacaagaaca tcgagacatt
      121 tcacatgaac gagggacatg ccggtttcct gaccctcgag ctcatgcggg agcagggata
      181 ctacgaccct gaaaaaatcc gggaccaggt gatcttcacc acccataccc cggttcccgc
      241 cggacatgat ttcttccgtt tcgacctggt ggacaaggtc atctcccagg aggccctttc
      301 gaacctgaag aggatgcttc ccaacgccga cggcgtatcc atgaccgaac tgggcatccg
      361 gttcagccgg tacgtgaatg ccgtgtcaaa aaaacacgcc gaagtgagcc ggaacatgtt
      421 caacatgccg gagatcgact gggtaaccaa cggcgtccac cccggaacgt gggtcagcag
      481 cggcatgaga cgcctcttca ccacccacat ccccggctgg gaacgggatc ccggagggct
      541 tgtccaggcg gtgaagattc ctcccgagga aatctggacc gcccaccagg cgtccaagat
      601 gcgcctcctc gccggcgtca tggagcagac aggccgccag ctcgaccccg acgtgctcac
      661 cataggtttc gccaggagag ctgcccagta caagagggcc gaccttcttc tctccaacat
      721 caggcgtctc atggaagtgg gggccggtaa aatacagctc gtcttcgccg gcaaggccca
      781 tccgagggac gacggaggca aaacggttct ccagaggctc ctcaccgcgt cccagaaggt
      841 agacgaaatg attcccatcg tcttcctgga gaattacgac atggaaaagg gcgccctcat
      901 cacccaggga gtggatctct ggctgaacac acccctccgt ccnnnnnnnn nnggaagcgt
      961 cgggcacgag cggcatgaaa tgcgccctga acggcgtgat gaatttttcc gttctcgacg
     1021 ggtggtggat cgagggctgg ctggaggatg tgacgggatg gtccgtgggg cccgagccca
     1081 gcgagtccga catgatgcac tacgatgaat ccctggatgc catcgacctg tacgacaaac
     1141 tcgagaaaaa aataatcccg acctactacc acaacaggga aaaatggatc tggatgatgc
     1201 agcggaccat cgccctcaac ggcagctttt tcaataccca cagggtaacc cgggaatact
     1261 gcgagaaggc atacaatatc agtttccggg ggatgtgatt cccatgaccg cagtccaggg
     1321 aggaagaacg ccgaaaatcc ttcacgtcag ccccgaagcc gtgcccttca gcaaggtggg
     1381 cgggctgggc gacgtggccg ggtcccttcc cgcggcgctt cgcgacagcg ggacggactg
     1441 caggctgctc accccggcct gggaaggggt gctcgacagg gcgagagaac tgggtttctc
     1501 cctttcgagg ctctccagga aggccgaggc ggtcatccgc tggaagattc acagggggac
     1561 ggtatggaaa tgttccggtg aaggcttggt ggcctacctg ctggaagagc cttccctttt
     1621 cggcaaaacc gtctaccccc gggagctgac ccctgattcg gtcactccgt tcctctttct
     1681 ctccctcgcc gccctggatc tcccctctgc gaccctgtgg aagcccgaca taatccactg
     1741 ccacgactgg ggaaccgccc ctcttccggc ggccctgaag tggcatatcc acttcgggtc
     1801 atcaggagat gtctgcaaaa ccgtcttcac catccacaat ctggcccacc aggggctcct
     1861 ttcccttgaa tccctgggcg actggggtat tcggaacgag gccgcccggg tggaaggcat
     1921 ggagtacttc ggcatggcca acctgatgaa aggagccctg gtttcctgcg atgcgattac
     1981 cacagtcagc ccgggctacg cggaggaaat tctcacggag gaaggcggag agggactggg
     2041 agggctgctg aagagccttt ccggcaaggt ctccggaata ctgaacggtc ttgacgaccg
     2101 gtactgggac ccccggaccg accctcttct tccggaacgg tattcccccg acaatctgtc
     2161 ggggaaaacc gcctgcaaaa agaaactgct tgaaagagcg ggctggaagg acgacgggcg
     2221 tcccctgctg gtttccgttg gaagaatggt ggaacagaaa ggtttttcca tccttcttcc
     2281 cgccctcgaa gagctcgtcg gaatgaactg ccgtcttttt ctcgtcggct cggggcagac
     2341 cgaatacgaa gaagcagccg acagcgcagc cgcccgtttt cccggttcgg tgttcgtctt
     2401 caggggctat gatgagcctc tggcccacct ggcatacgcc gggggggatt ttttcctcat
     2461 gccttcgaga ttcgaaccct gcgggctgtc ccagctcatt tccctcaggt acggcactgt
     2521 acccattgta agggccgcag gcggcctgag ggacaccgtc tttgaacacg gcactcccgg
     2581 aggaaacggc tttctcttct ccgggtacac ccccgaagac ctccttgaag ccgtcgccag
     2641 ggccatggag gtcttccggg acaaggattc catgcaacta ctctccctga ggggcatgaa
     2701 cgccgatttt tcatggaacc ggtcggcccc tctctataaa aaactgtacg aatcccttct
     2761 cctgtgacgg ctgtccgcca cggaagggaa agaatcttcg aaccgacatt cccaaacagc
     2821 cgaagggggt atgctccatg caggtaggca agtacggccg caccctgggc atcgtcctgg
     2881 cgggaggcaa aggtgaacgg ctcatgcctc tgaccaagta cagggcaaag cccgcggtcc
     2941 actttgcggc aaaatacagg atcgtggatt ttgcgctctc gaacctcgtc aacagcggaa
     3001 ttttttccat ttatgttctt gtccagttca aaagccagtc cctgaacgaa cacatcgaac
     3061 gaggctggca gttcggaggc gccctccggg gccgtgattt cttcatcacc ctggtgccgg
     3121 cccagatgtg gagaggcgag cactggttcc agggaacggc ggacgccgtc taccagaacc
     3181 tccatctcgt gaccctctac gatgcggacc gggtggccat cttcgcagcg gatcacattt
     3241 acaagatgga cgtggaccag atgctggcct accacatcgg caaggaagcg gacatcaccg
     3301 tggcggccaa cgtggttccg acatctgaag catcagcctt cggctgcatt gatactgacg
     3361 cagccggacg gatcgttgga tttgtggaaa aaccggagaa tcctcccgag attccgggca
     3421 acccgggctt cacctacgtt tccatgggca actacatctt cgaacgggag atcctcgaag
     3481 aatcccttat cgaagacgcc aaactgccaa cgagccacga cttcggcaga gacatcatcc
     3541 cgaagctcgt cgggacatcc aggatctttg cctatgactt ctccaccaac gttcttccgg
     3601 gaaaggaaaa atcatccttc tggagggagg acaagcccta ctggaaggac gtgggaacta
     3661 tccgggccta ctgggacgcc cacatggacc ttctgaagca cgactcggag atgaccttct
     3721 acaaccccca gtggcccata cggaccgtct cctatgccga tcccccctcc tacgtctgcc
     3781 ccgtgaaggg ctacacctgc aacgtgcaga gcaccctgtc cgccgaaggc agcagggtgc
     3841 tgggatccac cgtggaatcg tcggtactgt cccggaactg cgtcattctg cccggctcct
     3901 ctgtggagga gtccatcatc ggcgaaggtg tcgtggtggg agagaactgc aggctgcggc
     3961 gggtaatcat cgacagccac aacatcatcc cccccaacac ggtcatgggc tacgacgagg
     4021 agcatgaccg ggaacggtac accgtggacc ccgga
//
LOCUS       DAOTHD010000061         4045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_26298_length_4045_cov_3.603509, whole genome shotgun sequence.
ACCESSION   DAOTHD010000061 DAOTHD010000000
VERSION     DAOTHD010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26298_length_4045_cov_3.603509"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..777)
                     /locus_tag="P5219_01785"
     CDS             complement(100..777)
                     /locus_tag="P5219_01785"
                     /inference="COORDINATES: protein motif:HMM:NF039842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HRX25515.1"
                     /translation="MNTTGANGPFRFMECSLLPVSTGFRAQTLRELRSAVPEIPESSL
                     YYHFWGRMLRPHIAESEFNNDFASWADGGLGDTELAEALSAVNPVKCKDLGELRSILE
                     DILEARLDQGDFLSWKKADREFYFIACKKFMFDTGKVTSSLEDFPVAVKTASRQSFFH
                     HFLDGRRRSPEGKDDFSLWLEQFGEGTAEVRRLLGRVDSYLFSLGELKGQIVSILEKG
                     LRKGGKK"
     gene            complement(1004..1873)
                     /gene="secF"
                     /locus_tag="P5219_01790"
     CDS             complement(1004..1873)
                     /gene="secF"
                     /locus_tag="P5219_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRX25516.1"
                     /translation="MRTFFIDFMKHRKAALMLSLLCIAASLGLIFVKGLNLGIDFTGG
                     NVVQVEFTQPTAIEDIRQVLTSVGQGGSVIQSYSDRGVIIRISANEEEARRQTVESLR
                     SKFPGMQVIRLEKVGPVVGEELRQEAFIALILALAGILAYITVRFQFRFAVVSVAALL
                     HDAVITLGIFSLTGMEISSSFIAAILTIVGYSLNDTIVVLDRVRENWKNLRGTGIVDL
                     LNSSINQTLSRTINTSVTTLLPVVALYLWGGEVLRSFSFALLVGILVGTYSSIYVASG
                     LLAEWWSKVPQKG"
     gene            complement(1892..3289)
                     /gene="secD"
                     /locus_tag="P5219_01795"
     CDS             complement(1892..3289)
                     /gene="secD"
                     /locus_tag="P5219_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRX25517.1"
                     /translation="MLRRDRWRLGIVAIVVVAALVTVFPISGKINLGLDLKGGAHIVL
                     QAKESADSKVTEDSIERLLAVLRNRVDQYGVAEPVIQREGRDRVIVDLPGIQDPDAAL
                     ELIGKTAELEFRSVVESTASVPPGPHRENYESDEEFQRAQTRWNEAKAQIESGRESLA
                     KKGEGLAGSRVSRDDEGRYYLLGPVLVGGKDLVDAKTQYDNLGRAVVGLTFSSEGAKL
                     FDRATADNVGRQIAIVLDGVVISAPVVQERITGGNAQISGRFSPAEASRLSIMLRAGA
                     LPIAVEVIENRSVGPSLGEDSINSGIRAGLIGASLVFLFMLLYYRTLGIAADAALAVS
                     ILLVFAGLISLKATLTLPGIAGIVLTIGMAVDGNVLIYERMREETKVGKTPLAAVDAG
                     FKKALTTILDANITTLIAAAVLFYFGSGPVRGFAVTLSIGIVASVFCNVLVTRALLQH
                     FMGGRGAAGSMLHRS"
     gene            complement(3408..3779)
                     /gene="yajC"
                     /locus_tag="P5219_01800"
     CDS             complement(3408..3779)
                     /gene="yajC"
                     /locus_tag="P5219_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRX25518.1"
                     /translation="MEQQCGQAAGGAGGQGSLMGMLFPLAIFVVIFYFFIIRPQKKRQ
                     KAQDQLLASLTRGDQIVTIGGFFATIREVKDDSIVVEIADGVKVRILKSAVATKRAAA
                     APQQKESAPRDESGTAEEEKN"
     gene            complement(3848..>4045)
                     /locus_tag="P5219_01805"
     CDS             complement(3848..>4045)
                     /locus_tag="P5219_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HRX25519.1"
                     /translation="EVIIITVPGTAAQHVADKALQAGTVKGILNFAPVSLSVPGDVLL
                     AAVDISVELEKLLFFLKHKDE"
BASE COUNT          844 a         1174 c         1163 g          864 t
ORIGIN      
        1 gaacaatccg ctttccccgg agacgctccg ctatggccag aaggctgtcc atggcatgga
       61 agccgataac ttcgccgtac tgcttcagca tgtcggacat catttcttcc ctcccttccg
      121 gagtcccttt tcgagaatgg agacgatctg tcccttcagt tcaccgaggg aaaaaaggta
      181 cgaatccacc ctgccgagga gccgcctcac ctccgcggta ccctcgccga actgttcaag
      241 ccacagggag aagtcgtcct ttccctcagg agaccggcgg cggccgtcga gaaagtgatg
      301 aaaaaagctc tgccggctcg ccgttttgac cgccacggga aaatcctcca gggaggaggt
      361 aacctttccc gtatcgaaca tgaatttttt gcaggcaata aaatagaact ccctgtccgc
      421 ctttttccag ctcaggaaat ctccctggtc aagtcgggcc tcgagaatgt cctcaaggat
      481 agaccgtagt tccccaagat ccttgcattt cacgggattg acggcgctga gggcttcagc
      541 gagttccgtg tccccgaggc ctccgtcggc ccacgaggca aaatcattgt tgaactccga
      601 ctcggcgatg tgggggcgga gcattctgcc ccagaaatga taatagaggg aactttccgg
      661 aatttcggga acggcggacc tgagttctct gagcgtctgt gctcgaaagc ccgtgctcac
      721 gggcagaaga gagcattcca taaatctgaa ggggccgttg gctcctgtag tgttcatggc
      781 ttcaacctcc tttgtgcagt cgatgtattc atctcccttt cattatgtag ccttatcggc
      841 attgaataag tataacacat aatttctttg ttctacaata tttctccttt ccttgtcatc
      901 ggcaggagcg tcaaagtatt gtggggaacc aggatttttt cggcatggat gcgggggaaa
      961 aaaaggagcc cccagagggg gctcccgtac tgcggaagaa tgatcagcct ttctggggca
     1021 ccttgctcca ccactccgcg aggaggccgg aggccacgta aatggagctg taggtcccca
     1081 ccaggatacc caccaggagg gcgaaggaga aactcctgag cacctctccg ccccagagat
     1141 agagtgccac cacgggaagc agggttgtga ccgacgtgtt gatggttctg gaaagcgtct
     1201 ggttgattga agagttgagc agatccacga ttcccgttcc cctgaggttt ttccagtttt
     1261 ccctgacgcg gtcgagaacg acgatggtgt cgttcagcga atatcccacg atagtgagaa
     1321 tggcggcgat gaaggacgag gaaatttcca tgccggtgag gctgaaaatt ccaagggtga
     1381 tgacagcgtc gtggagaagg gctgccacgc tcaccacggc aaagcggaac tggaaccgga
     1441 cggttatgta agcgagaatg cccgccaggg cgaggatcag ggcaataaaa gcctcctgcc
     1501 gcagttcttc tcccactacc ggaccgacct tttcgagccg gatgacctgc atgcccggaa
     1561 atttcgaccg cagggactcc acggtctgcc tgcgcgcctc ttcttcattg gcgctgatcc
     1621 ggatgatgac cccccggtcg ctgtaggact ggatgacgga acctccctgg ccgacggagg
     1681 tcaatacctg gcggatgtcc tcaatagccg tgggctgtgt gaattctacc tgcaccacgt
     1741 tccccccggt aaagtcgatg ccgaggttca gtcccttgac gaaaatgagc ccgaggctcg
     1801 ccgcgatgca aagaaggctg agcatcaggg ctgccttcct gtgtttcatg aagtcgatga
     1861 aaaaagttct catggtcgtc tttcctccct ctcaggagcg gtgcagcatg gaaccggccg
     1921 caccccgtcc tcccatgaag tgctggagga gcgccctggt gacgagtacg ttacagaaaa
     1981 cgctcgccac gatgccgatg ctgagggtca ccgcaaaacc gcggacaggt ccgctgccga
     2041 agtagaacag cacggcggca gcgatgagcg tggtgatgtt ggcgtccaga atggtcgtca
     2101 gggccttctt gaagcccgcg tcaacggcgg ccaggggcgt ctttcccacc ttggtctctt
     2161 ctctcatccg ttcatagatg agcacgttgc cgtccacggc catgccgatg gtcagcacga
     2221 tacccgcgat tccggggagg gtgagggtgg ccttgaggga gataaggccc gcgaagacga
     2281 ggagaatgga cacggcaagg gccgcgtcgg cggcaatgcc gagggtcctg tagtagagca
     2341 gcatgaagag aaacacgagg gaggcgccga taaggcccgc cctgattccg gagttgatgg
     2401 agtcctcgcc gaggctcggc cccaccgacc tgttctctat gacttcgacg gcgatgggga
     2461 gcgcgccggc ccggagcatg atggaaagcc ggcttgcctc cgcggggctg aaccgtcccg
     2521 aaatctgggc atttccgccg gtgatccgct cctgaactac gggagcggat atgaccactc
     2581 cgtcaaggac aatggcgatc tgccgtccca cgttgtcagc cgttgccctg tcaaagagct
     2641 tggccccttc ggagctgaag gtcagtccca caacggcccg gccgaggtta tcgtactggg
     2701 tcttggcatc aacgagatct tttccgccca cgagcacggg accgagaagg taatacctgc
     2761 cttcgtcatc cctggaaacc cgggaaccgg cgagaccttc gccttttttc gcaaggcttt
     2821 ccctgccgct ctcgatctgg gccttggcct cgttccaccg ggtctgggcc cgctggaact
     2881 cttcgtcgct ttcgtaattc tcccgatggg gccccggagg caccgaggcc gtcgattcca
     2941 cgacggaccg gaactcgagt tcggcggtct tgcctatgag ttcgagggcc gcatcaggat
     3001 cctgtattcc gggaaggtca acgataaccc tgtccctgcc ttccctctgg atgaccggtt
     3061 cggccacgcc gtactggtca accctgttcc gaaggacggc aaggagcctt tcgatgctgt
     3121 cttcagttac cttgctgtcg gctgattctt ttgcctgtag gacgatatgg gctccgccct
     3181 tcaggtccag cccgaggttg atctttcccg aaatgggaaa caccgtcacg agagccgcaa
     3241 ctaccacgat ggcgacgatt ccgagccgcc agcggtctct tctgagcatt attcttccct
     3301 cctggacact ccgcgaaatt agtgcatatc aaaagaccgg aaaaagcgga gcggccgccg
     3361 gaaggcgacc ctctccgcac cggtgtcgtt gttcacctgg gaaaaggtca gttcttttcc
     3421 tcttcggcag taccggattc gtccctgggg gcggattcct tctgctgggg cgcggccgcg
     3481 gcgcgcttgg tggccactgc ggatttcagt atccggacct tgactccgtc ggcgatctcc
     3541 acgacgatgc tgtcatcctt gacttccctt atggtggcga aaaatccgcc gatggtgacg
     3601 atctggtcgc ccctggtgag ggacgccaga agctggtcct gggctttctg ccgcttcttc
     3661 tggggcctga tgatgaagaa atagaagata acgacgaata tcgcgagagg gaaaagcatt
     3721 cccataagac tgccctgtcc tccagctcct cctgctgcct gtccgcactg ctgttccata
     3781 aacaaacgcc tcctcaaaga aatttggttc gaaggccccg accgggacca tgatattcta
     3841 tcaaatacta ctcgtctttg tgcttgagaa agaaaagcag tttctcgagt tcaacggata
     3901 tatcgacggc ggcgagaagc acatctcccg gaacggaaag ggacaccggg gcgaagttga
     3961 gaattccctt gacagttccc gcctggaggg ccttgtcggc cacatgctgc gccgcagtcc
     4021 ccggaacggt gatgatgatc acttc
//
LOCUS       DAOTHD010000062         4045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_26299_length_4045_cov_2.842105, whole genome shotgun sequence.
ACCESSION   DAOTHD010000062 DAOTHD010000000
VERSION     DAOTHD010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26299_length_4045_cov_2.842105"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="P5219_01810"
     CDS             complement(<1..682)
                     /locus_tag="P5219_01810"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRX25520.1"
                     /translation="MRKGIIALAVLALFLAAGAAFAADVKEVYSENGMVSSAHELASK
                     AGVEILQKGGNAIDAAIATMLALNVVEPNASGIGGGGFSTIRFAKTGEVVELDYREVA
                     PLSSTKDMYASEESKKAKESVLGGKAVGVPGIVKGIFTALEKYGTMSFAEVAAPAIRL
                     AEEGFEVHPMQNQIITDEFGKLSKYSPDSVFLPGGLPAETGSILKQPELAKAFRLLAS
                     DGPDAFYNG"
     gene            complement(789..1922)
                     /locus_tag="P5219_01815"
     CDS             complement(789..1922)
                     /locus_tag="P5219_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HRX25521.1"
                     /translation="MKLKGTPLTAVLLLALALGLSALAAVQFRAMWADDIFVPASGFE
                     KHLLSEWFGGIRDTPADTPVYIQEGTEPGGTVLILGGTHPTEPASMMTATLFLERAKV
                     TKGRLIVIPQANIMGFTHNSPQEAHPRSISFAAADGSRRTFRFGSRLTNTVHEWPAPD
                     IYIHPASGQQLPGKERGNLNRCYPGVPDGSLTERLAYALTELVRKEKADLSFDLHEAS
                     PEYPVVNAIVAHERSMELAAMAAMDLEGKGVPIRLEPSPKNLRGLTHREWGNYTETMP
                     ILMETGNPVQGRLRGKTDERLALTGVDKAYVKAFALGRLYIPYDGKQTMEYRVGRHAE
                     SILSFLDNLDILFEERGVRVEGVPGLAELEEKGVGFFLAPVKQ"
     gene            complement(1936..2079)
                     /locus_tag="P5219_01820"
     CDS             complement(1936..2079)
                     /locus_tag="P5219_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25522.1"
                     /translation="MTYVYRAIVLFILLLVVNCQFREKDFWKQATAALVVIPLVLRLL
                     MIK"
     gene            complement(2092..3381)
                     /locus_tag="P5219_01825"
     CDS             complement(2092..3381)
                     /locus_tag="P5219_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRX25523.1"
                     /translation="MDWFFPEGLYTLAMVGSFVFGAFALKLPIAVALGAAAAVGALAA
                     GEGFPVRHFVEGTFGYLDTILIIATAMIFMKTVQKTGLLESLAAWVIRTFRTRPLLLS
                     LGLMVIIMVPGMITGSSTAAVLTTGALVAPVLIKLGVPAVKTAAAIAMGAIYGMIAPP
                     VNVPAMIIGGGIDMPYVGFALPLLVCTVPLAFFSALVLVYPHLKKGTGDENALEEELR
                     RMEAVPLSFRLVLPVILLAVLMTGEQFLPGIWPSLGMPVNFLLAALSALFTGRRLNFL
                     EASREAVNDAIPVLGILMGVGMFIQIMTLVGVQGFIVVSVLALPAWLMYLGMATSIPL
                     FGAVSAFGAASVLGVPFLLALLGQNEIMVGSALSLLAGLGDLMPPTALAGIFAAQVVG
                     EENYFRVLRHCIVPAVVTAAWAVVVILNANTIMGWLP"
     gene            complement(3400..3951)
                     /locus_tag="P5219_01830"
     CDS             complement(3400..3951)
                     /locus_tag="P5219_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HRX25524.1"
                     /translation="MKRLIIALTALLVLGAAGAAFAADVVLTSVGQSPDVMMVRVVLR
                     KMKIDADSEPLLKADALAGRKVLVAVVGGSSKGLGAAGINKDQEVERVSSLLKAAKEQ
                     GVKVLVMHIGGEGRRGTLSDAFIEAAAPWGDRIIVVDGGNSDGIFEKLTEGKNVKILV
                     APNVNGTSAPLGETLSEWGISAK"
BASE COUNT          822 a         1208 c         1203 g          812 t
ORIGIN      
        1 ggccgttgta gaaggcgtcg ggcccgtcgc ttgccagaag tctgaaggcc ttggcaagtt
       61 cgggctgctt caggatggag ccggtctcgg cgggaaggcc gccgggaagg aatacgctgt
      121 cggggctgta cttgctcagc ttcccgaatt catcggtgat gatctggttc tgcatgggat
      181 ggacctcgaa gccttcctct gccagccgga ttgccggagc cgcgacctcg gcgaaggaca
      241 tggtgccgta cttttcaagg gcggtgaaga tacccttcac tatgccgggc acaccgacgg
      301 ccttgccgcc gaggacggat tccttggctt ttttggattc ctcggaagcg tacatatcct
      361 tcgtggagga caggggagcc acttcacggt agtcgagctc caccacctcg ccggtcttgg
      421 cgaaccggat ggtggagaat ccgccgccgc cgattccgga agcgttgggt tccaccacgt
      481 tgagggcgag catggtggca atggccgcat cgatggcgtt tccgcccttc tgcaggatct
      541 ccacgcccgc tttcgacgca agctcgtggg ccgaggacac catgccgttt tcagaataga
      601 cctccttcac gtccgcagcg aaagcggccc ctgccgccag gaacagcgcg agtactgcca
      661 gtgcaataat tccttttctc acataaaaca cctccctgta atgtgtgttc atgattctcc
      721 gtacgggctg cacgcctccg ctttatgcag aagcaattca gtattttcta ttgtacagta
      781 aagggagcct attgttttac gggggccagg aagaacccga ctcccttttc ctcaagctcg
      841 gcaagtcccg gaacgccttc cacgcggact cctctttcct cgaaaaggat gtcgagattg
      901 tccaggaagg aaagaatgga ttcggcatgc cgtcccaccc tgtactccat ggtctgcttc
      961 ccgtcgtagg gaatatagag cctcccgagg gcaaaagcct tcacgtaagc cttgtcgacc
     1021 ccggtgaggg caagccgttc gtccgtcttt ccacggagcc ttccctggac cggatttccc
     1081 gtttccatga ggatcggcat ggtttcggtg taattgcccc actcccggtg ggtcagcccc
     1141 ctcaggttct tcggcgaagg ctccaggcgg atggggacac cctttccttc caggtccatg
     1201 gcggccatgg cggccagctc catgctccgt tcatgggcca cgatggcgtt caccaccggg
     1261 tattcaggag atgcctcgtg aaggtcgaag gaaagatcgg ccttttcctt tcggacaagc
     1321 tccgtaaggg cataggcaag gcgctccgtg agggacccgt cgggaacgcc cggatagcat
     1381 ctgttgagat tgccccgctc ctttccggga agctgctgcc ccgaggcggg atggatgtag
     1441 atgtccgggg ccggccattc gtggactgtg ttggtcaggc gggatccgaa gcggaacgtc
     1501 cgcctgctcc cgtcggcggc ggcgaaggag atgctccggg gatgggcttc ctggggagaa
     1561 ttgtgggtga agcccatgat gttcgcctgg ggtatgacga tcagccgtcc cttcgttacc
     1621 ttcgcccgtt cgaggaacag ggtcgccgtc atcatggacg ccggttcggt ggggtgagtt
     1681 cccccgagga tcagcacggt gcctccgggc tccgttcctt cctggatgta gacaggggta
     1741 tccgccggag tgtccctgat ccctccgaac cattcagaaa gcaggtgctt ctcgaagccc
     1801 gaggccggga caaagatatc atctgcccac atggcacgga actgtaccgc ggcaagggcg
     1861 gaaagcccga gagccagggc gagaaggagt actgcggtca atggggttcc tttcagcttc
     1921 atcgtccttc cctccctact tgatcatcag gagccggaga accagcggaa tgaccacgag
     1981 cgccgccgtg gcctgtttcc agaagtcctt ctcccgaaac tggcagttca ctaccagaag
     2041 aagaatgaag aggacgatag ccctataaac atatgtcatg tccgtttcct cctaaggcag
     2101 ccagcccatg atggtgttcg cgttgaggat caccacgacg gcccacgctg cggtgaccac
     2161 cgcaggaacg atgcagtgcc ggagcactct gaaatagttt tcctcgccga cgacctgggc
     2221 ggcgaaaatg cctgccagtg ccgtgggggg catgaggtcc ccgagacccg caagaagact
     2281 gagggcggac cccaccatga tttcattctg tccgaggagg gcgaggagaa agggaacgcc
     2341 caggaccgag gcggcaccga aggccgatac tgccccgaaa agggggatcg acgtggccat
     2401 gccgaggtac atgagccagg cgggcagggc gaggaccgac accacgatga agccctggac
     2461 gccgacaagg gtcatgatct ggatgaacat tcccaccccc atcaggattc cgagtaccgg
     2521 gatagcgtca ttgacggctt cccgggaggc ttcgagaaaa ttcagccgcc ttcctgtgaa
     2581 aagagcggac agagccgcga gaagaaaatt caccggcatt ccgagggacg gccagattcc
     2641 gggaagaaac tgttctcccg tcatgaggac ggcaagcaga atcaccggga gaacaagtct
     2701 gaaagagagc ggaactgcct ccatccggcg cagttcttcc tccagggcgt tttcgtcccc
     2761 tgttcctttt ttgaggtggg gatacacgag gacaagggcg gaaaaaaacg ccaggggaac
     2821 agtgcagaca aggagaggca gggcgaaccc aacgtagggc atgtcgatgc cgccgccgat
     2881 gatcatggcc ggcacgttca ccggaggggc gatcatcccg tagatggctc ccatggcgat
     2941 ggccgccgct gttttcactg ccggaacgcc gagctttatc aggacaggtg cgacaagagc
     3001 acccgtggtg aggacggccg ccgttgagga cccggtgatc atgcccggaa ccatgatgat
     3061 gaccataagt ccgaggctga ggaggagagg cctggttctg aacgtgcgga tgacccaggc
     3121 ggccaggctt tcaagcaatc ccgtcttctg gacggtcttc atgaaaatca tggccgtggc
     3181 gatgataaga atggtatcga gatagccgaa ggttccctcg acgaaatgcc ggacgggaaa
     3241 cccctccccc gcggcgaggg ctcccactgc cgcggcggct ccaagggcga cggctatggg
     3301 gagcttcagg gcgaatgccc cgaagacaaa gctccccacc atcgccagcg tatataatcc
     3361 ttcaggaaag aaccagtcca tgacgggagt accgtccgcc tatttggcgg agattcccca
     3421 ttccgaaagg gtttccccga ggggagcgct tgttccgttc acgttggggg caacgaggat
     3481 tttcacgttt ttaccctccg tcagcttctc gaagattccg tcgctgttgc ctccgtccac
     3541 gacaatgatc ctgtctcccc agggcgcagc cgcctcgatg aaggcatcgg acagggtgcc
     3601 tctgcggcct tctccgccga tatgcataac gagaaccttc actccctgct cctttgccgc
     3661 cttcagaagg gagctcaccc gctccacttc ctggtccttg ttgattccgg cggcaccgag
     3721 tcccttggag ctgcctccca ccaccgcaac cagtaccttc cggcccgcaa gggcgtcggc
     3781 cttcagcagg ggttcgctgt cggcatcgat cttcatcttc cggagaacca ccctcaccat
     3841 catgacgtcg ggactctgcc ccacggacgt aagcaccacg tccgccgcga aggcggctcc
     3901 cgccgctccg agcaccagga gcgccgtcag cgcaataatc agtcttttca tgtcgattcc
     3961 tccttctgtc aacagataga ttcctcttga gatactgaat agagtgtacc cttttacagt
     4021 tttatgtcta tatttcttat cattc
//
LOCUS       DAOTHD010000063         4044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_26310_length_4044_cov_3.399098, whole genome shotgun sequence.
ACCESSION   DAOTHD010000063 DAOTHD010000000
VERSION     DAOTHD010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26310_length_4044_cov_3.399098"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="P5219_01835"
     CDS             complement(<1..325)
                     /locus_tag="P5219_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRX25525.1"
                     /translation="MKIVVVGGGEVGYSVAETLSEEGHDVTVVEENEERAARVDSELD
                     VITVRGSGARPQVLEEAGIVPGCDVDILVACTDHDEVNILACWIAKRAGVKRVISRAR
                     GLEFTD"
     gene            complement(444..3152)
                     /locus_tag="P5219_01840"
     CDS             complement(444..3152)
                     /locus_tag="P5219_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRX25526.1"
                     /translation="MEERLEALRRLETREGMAVFDEGSLDGFLSGAHVGAAIISPELE
                     VIWSNSFFRQAFGCMEGMSCYGAFFHEEEPCAECGVREVTEGRSERYVARKKPRTSDE
                     WFQVVYTPLRAPSGKTVAARALALPLTDLLRTEEALRTTQSNLLAMLNNSLQSFVLLD
                     GDLRVVAFNREAEEDLAGLFGTRPEEGAFFLDLVSPPVARMLSSMLSFSPGEGRKREN
                     LAVSSNSGGIFHYEVETIPILDGEGKHAAGLLCARNVTDREQNRRHLEDLLAILPLIM
                     WSWDLRSSRLVQVSGACEEILGFSPEGFPGDGIPPEDFLCGEDRAEVKAFLDSVLKDG
                     RASGEYRVCRADGTFRWLFSRASLVLGHDGGALRVNGVSYDITSVRDLASRLSKTEAR
                     YRAVISTTSQGFLLHDLSVTESVNDAFCAMLGLAPEDILGKSPGTIVYPDDLPLWEEA
                     RGKILTTDHRNYPIRLRRKDGSPLPVQVSATTLRDGSGKAMGAFSFFTDLSERTAMEE
                     RLRDALSGAEEANRAKSAFLANMSHEIRTPMNAILGMTSLVLETALSQEQRELLEMVD
                     ASGKKLMTLLNDILDLSRIEAGKLSLAREPFSLRKTLDQVLRPLALSARQKDLFFTMD
                     VDPELGDRFLGDAIRLSQVLLNLCSNALKFTSRGGISVDVRSEDPKRPGLIRFSVTDT
                     GVGIREDQKEKLFGYFSQLDQSRSKQFEGTGLGLAISRELVTAMNGRIWVESREGQGS
                     TFKFTVLLELSEGQFAEKQGGEKDPPFRPAGRARRILLAEDNPVNAVFAERVLTSAGH
                     AVVRASSGQEALDILLGKGPFDLVLMDVQMPGMDGLEAVKRIREGEQQGEERLPVAAL
                     TAYALGEDRDLCLAAGMDDCITKPVSRDALLAAVERLTSRGGEGEG"
     gene            complement(3303..>4044)
                     /locus_tag="P5219_01845"
     CDS             complement(3303..>4044)
                     /locus_tag="P5219_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRX25527.1"
                     /translation="CFRNGFLGMVEGKAAGYTAHKLLGGKTVALLTSDNDFGRTLVIG
                     FKEYASKYAPDLKIVSEQAYPFSEKDYKPYLSKIKELNPDVIFASGYYFQTGPLLRQA
                     RELGIQSKILGEEGADSPKLMEIAGDAAEGFHIVTNFDRDDPRPVVQGFLKEFRGRHK
                     FEPDMVGASAYDAFMIIMDGMKTAGSTEGGKVRDAIAAVKDYNGLTGIIGGFDEIGEV
                     VKPVQVQVVKDGLFRHFGVVNDPELIKP"
BASE COUNT          732 a         1291 c         1221 g          800 t
ORIGIN      
        1 tgtcggtgaa ctcaagaccc cgggcccggg agatgacccg ttttaccccg gcacgcttgg
       61 cgatccagca ggcgaggata tttacctcgt catgatcggt gcaggccacg aggatgtcca
      121 cgtcgcaccc tggaacgatg ccggcttcct cgagcacctg gggccgggca ccgcttcccc
      181 tgacggtgat cacgtccagc tcgctgtcaa cccgggctgc ccgctcctcg ttctcctcaa
      241 cgacggtgac gtcgtgccct tcttccgaaa gggtctccgc cacgctgtag cctacttctc
      301 ctccgccgac gacgactatc ttcactcttt cattccctcc gccggcccgt tcattccggg
      361 cccattgatt gcgccgatac cataccctat ccggagaaaa aacaccatcc ccataccggt
      421 atacattgac gggatacgct ccgtcagcct tccccttctc ctccgcggga cgtgagcctt
      481 tccaccgcgg caagaagagc gtcccgtgac acaggcttcg ttatgcagtc atccatccct
      541 gccgcgaggc aaagatccct gtcctcgccc agggcatagg cggtcagggc ggccacggga
      601 aggcgttctt ccccttgctg ttccccttcc cggatgcgct tcaccgcttc cagcccgtcc
      661 atgcccggca tctggacatc catgaggaca aggtcgaaag ggcctttccc gagcaggatg
      721 tccagggcct cctgcccgga ggatgcccgc accacggcgt gcccggcgga ggtgagcact
      781 ctttccgcga agacggcgtt caccgggttg tcctcggcga gcagtattct ccgtgcccgt
      841 cccgccggcc ggaaaggagg gtccttttct cccccctgct tttcggcaaa ctggccctcg
      901 gaaagctcga gaagcacggt gaatttgaag gtgcttccct gcccttcccg gctttcaacc
      961 cagatgcgtc cgttcatggc tgtcacaagc tccctggaaa tggcgagccc gagcccggtt
     1021 ccctcgaact gcttcgacct ggactggtca agctgggaaa aatagccgaa aagcttttct
     1081 ttctggtcct cccggatccc tacgcccgta tccgtgactg aaaagcggat cagtccggga
     1141 cgttttgggt cttcagagcg cacgtccacg gagatgccgc cccgggaagt gaatttcagg
     1201 gcgttgctgc agaggttcag aagcacctgg ctcaaccgga tggcatcgcc gagaaacctg
     1261 tcgcccagct caggatccac gtccatggtg aaaaacagat ccttctgccg ggccgagaga
     1321 gccaggggcc tgagaacctg gtccagggtt ttccggagcg aaaagggctc tctcgcgagg
     1381 ctcagttttc ccgcctcgat ccgggagagg tccaggatgt cgttcagcag ggtcatgagt
     1441 ttcttcccgg aggcatccac catctcaaga agttccctct gctcctggga gagggccgtt
     1501 tccagcacca gggaggtcat gccgagaatg gcgttcatgg gtgtccggat ctcgtggctc
     1561 atgttcgcca ggaaggcgct cttggcccgg ttggcctcct cggctccgga cagggcgtcc
     1621 ctgagccttt cctccatggc cgtccgctcc gacaggtcgg tgaagaagga gaaggctccc
     1681 atggcctttc cggagccgtc ccggagggtc gtggcgctca cctggaccgg aaggggagag
     1741 ccgtccttcc gcctgagccg gatagggtaa ttcctgtgat cagtcgtgag gatttttccc
     1801 cgggcctcct cccacagggg aaggtcgtcg ggatagacga tggtgccggg acttttcccg
     1861 agaatgtcct ccggtgcaag gccaagcatg gcgcagaaag cgtcgttcac cgattccgtc
     1921 acggagaggt cgtggagcag aaaaccctgg gaggtggtgc tgatcaccgc cctgtacctc
     1981 gcctcggtct tcgagaggcg ggaagccagg tccctcacgg aggtgatgtc atagctcacg
     2041 ccgttcaccc ggagagcccc gccgtcgtga ccgagaacaa gggaggccct ggagaaaagc
     2101 caccggaacg ttccgtcggc ccggcatacc ctgtattctc ccgatgccct tccatccttc
     2161 aggacggaat cgaggaaggc tttcacctca gcccggtcct caccgcagag aaaatcttca
     2221 ggcggtatgc cgtcccccgg aaaaccttca ggtgagaagc cgaggatttc ctcgcaggct
     2281 cctgaaacct gcacgagacg ggaggaccgg aggtcccagg accacatgat caggggcagg
     2341 atggccagca gatcctcgag gtgccgccgg ttctgttccc tgtcggtcac gttccgggcg
     2401 cacagcaggc cggccgcgtg ttttccctcc ccgtccagga tgggaattgt ctccacttcg
     2461 taatgaaaga tgccgccgct gttggaggag accgccagat tctcccgttt ccttccctct
     2521 cccggagaga aggagagcat ggaggagagc atccgggcaa cgggaggaga gacaaggtcg
     2581 agaaagaagg ccccctcctc ggggcgggtt ccgaaaagcc ccgccaggtc ctcttccgct
     2641 tcccggttga aggcgacgac acggagatcc ccgtcgagaa ggacgaagct ctggagggag
     2701 ttgttcagca tggcgagcag gttggactgg gtcgtcctga gggcttcctc ggtgcggagc
     2761 aggtccgtga ggggcagcgc cagggcccgg gcggcgacgg tttttcccga gggagcccga
     2821 aggggggtgt agacgacctg gaaccattcg tccgacgtcc tgggcttctt cctggccacg
     2881 taccgttcgg accgcccctc cgtcacttcc cggacgccgc attcagcgca gggttcctct
     2941 tcatggaaaa aggccccgta acaggacatg ccctccatgc acccgaaagc ctgccggaaa
     3001 aaggagttcg accagatgac ctccagttcg ggagagatga tcgccgcacc cacgtgggcg
     3061 cccgagagaa agccgtcaag gcttccctcg tcgaaaacag ccattccttc ccttgtttcg
     3121 aggcgccgaa gcgcctcaag ccgctcttcc atggccggac ctcccctggt atcctcttca
     3181 tgttcagttt attctaccct acgcctccgg gaaggaaaaa gatcccgttc cggaaaaaaa
     3241 gcgccttccc ttaagggaag gcgctttccg tcctgttttg cccgttgata cggatgtcag
     3301 cctcagggct tgatgagttc ggggtcgttc acaacgccga agtgccggaa caggccatcc
     3361 ttcaccacct gcacctggac gggcttgaca acttcgccga tctcgtcgaa gccgccgatg
     3421 atgccggtga ggccgttata gtccttcacg gcggcgatgg cgtcccggac cttgccgcct
     3481 tcggtgcttc cggcggtctt catgccgtcc atgatgatca tgaaggcatc gtaggcggag
     3541 gcgcccacca tgtcgggctc gaacttgtgc cggccccgga actccttcag gaagccctgc
     3601 accacggggc ggggatcgtc ccggtcgaag ttggtgacga tgtggaaccc ttcagctgcg
     3661 tcgccggcga tctccatgag cttcggggag tcggcgccct cctcgccgag gatcttggac
     3721 tggatgccca gttctctggc ctggcgaagg agcggcccgg tctggaagta atagccgctg
     3781 gcgaagatca cgtcggggtt gagctccttg atcttcgaga gatagggctt gtaatccttc
     3841 tcggagaagg ggtaggcctg ttcgctgacg atcttgaggt cgggggcgta cttggaggcg
     3901 tactccttga acccgatcac gagggtccgg ccgaagtcat tgtcgctggt caggagggcc
     3961 accgtcttgc cgcccaggag cttgtgggct gtgtatcccg ccgccttgcc ctcaaccatg
     4021 cccaggaagc cgttccggaa gcag
//
LOCUS       DAOTHD010000064         4024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_26472_length_4024_cov_3.295540, whole genome shotgun sequence.
ACCESSION   DAOTHD010000064 DAOTHD010000000
VERSION     DAOTHD010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26472_length_4024_cov_3.295540"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..414
                     /locus_tag="P5219_01850"
     CDS             73..414
                     /locus_tag="P5219_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HRX25528.1"
                     /translation="MRVSEIGDCLDLTVCSPGDPAREIGGVVSGDLLSFIMAEAREGW
                     MWITIQVHLNVAAVAVLKDVPFILIASGRKPEKELVDRCLSEGITLACTRLSAFEAAG
                     RLWEAGLGRPS"
     gene            480..1196
                     /locus_tag="P5219_01855"
     CDS             480..1196
                     /locus_tag="P5219_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HRX25529.1"
                     /translation="MGAPEIVEAYREAGIALCAVTDHNASDNVMAVAGAAQGIPVVLP
                     GMEVQTAEDIHIVVIFPGLSEAGDFQAWLWKKMPPVANRPEIFGDQLIIDKNNSITGE
                     QEVLLVQGAGYTADETAAEARSRGGLVILAHLDRPSFSYEAVLGPVPDDFPCHALELS
                     PAVSHSKFSGWRERYPDRVFIRSSDSHRLSTISRDRCTAMMLESPCFREIKLALCGQE
                     GRSVLCPWGDGLSWRRSSSA"
     gene            complement(1127..1783)
                     /gene="hisIE"
                     /locus_tag="P5219_01860"
     CDS             complement(1127..1783)
                     /gene="hisIE"
                     /locus_tag="P5219_01860"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HRX25530.1"
                     /translation="MAFSLDDVKFGSDGLVPVIVQDVLSGEVLMMAWANREALEATLS
                     SGMLTFWSRSRGTLWKKGETSGNTLALQELRLDCDGDALLALAEPAGPACHTGERSCF
                     HRLLTTPCEGRGTFPGILWRYLEKRRHDDPAESYTARLLSRGLRRVAQKVGEEGVETA
                     LAVAAGDRKETICEAADLLYHLEAALLASGVSPREIYAELERRHDKPSPQGQSTERPS
                     "
     gene            complement(1790..2551)
                     /gene="hisF"
                     /locus_tag="P5219_01865"
     CDS             complement(1790..2551)
                     /gene="hisF"
                     /locus_tag="P5219_01865"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRX25531.1"
                     /translation="MLTKRIIPCLDVKEGRVVKGVNFVNLRDAGDPAAMAAAYMEEGA
                     DELVFLDITASAERRRTMTAWVSAVADALFIPFTVGGGISSVEQAREIVALGADKISL
                     NSAAVRNPSLIGECAALLGKQAVVLAVDARRCGVEKWEVFIDGGRTATGLDALKWIEE
                     GVRRGCGEILLTSMDRDGVKTGYDDDLNGLASGSVHVPLIASGGAGSMEHILGTFRAG
                     SDAALAASIFHFGEIRIPDLKRYLAERGVPVRPSR"
     gene            complement(2545..3264)
                     /locus_tag="P5219_01870"
     CDS             complement(2545..3264)
                     /locus_tag="P5219_01870"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HRX25532.1"
                     /translation="MELFPAMDLYQGEIVRLRQGRFEERTVFGSDIAGTARSFREEGA
                     EWIHVIDLEGAKKGFPCHLSAIGMLLDAGLKVQYGGGLRTEDHLEAAFSAGAERIYSG
                     SLFASDPAGGNILFRRFGNRVIPAVDIRNGEVAVSGWQETAPFSPEQLVEHIAASGFT
                     LFLVTAVHRDGTGTGPDLEMYRSLRNSFPDLSIIAAGGISSLENIRSLAGAGMAGAVL
                     GRALYEGTVQLGDALKEASGC"
     gene            complement(3264..3881)
                     /gene="hisH"
                     /locus_tag="P5219_01875"
     CDS             complement(3264..3881)
                     /gene="hisH"
                     /locus_tag="P5219_01875"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRX25533.1"
                     /translation="MIAIVDYQVGNTGNVRRAFSSLGEEARLLASPAELTAEYTLAVL
                     PGVGAFGPATARLRASGWADRLISWASEGKPLLGICLGMQLLCERGLEDGDHPGLGLI
                     PGEIRLLKAEKIPHMGWNSVEWTGQPPGFVTAVPSGEYFYFVHSYALFDSAFAAGRTS
                     LGGANFTSMVLRGRIAGFQFHPERSGRAGLALLSEAADFIRKEVP"
     gene            complement(3878..4018)
                     /locus_tag="P5219_01880"
     CDS             complement(3878..4018)
                     /locus_tag="P5219_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25534.1"
                     /translation="MTLHAQALACDNAHHLAEALFKGAGRALRDAFLPSENLQSTKGT
                     LV"
BASE COUNT          748 a         1279 c         1206 g          791 t
ORIGIN      
        1 cggagagatc caggcccttt cctcaaagct gccccacacc cttcacccct ccggcaaaag
       61 gaggcagaga tgatgagagt ttcggaaata ggcgactgcc tggatctcac cgtctgctca
      121 ccaggtgacc cggccaggga gatcggaggc gtcgtctccg gggatctgct gagctttatc
      181 atggcggagg ccagagaagg atggatgtgg atcaccattc aggtccacct gaacgtggcg
      241 gcggtggcgg tcctcaagga tgtgcccttt atcctcatag cttccgggag aaagccggaa
      301 aaggaactcg tcgaccggtg cctttccgaa ggcataaccc ttgcgtgtac ccgtctgtct
      361 gccttcgagg ccgccggaag gctctgggag gccggcctgg gaaggccgtc atgagccttt
      421 cccccttttg ggtggatctt catcttcata cagttctttc tccctgcgga gagctggaaa
      481 tgggtgcccc ggagatcgtg gaggcctacc gggaggcagg tatcgccctc tgcgccgtga
      541 cagaccacaa cgcctcggac aacgtgatgg ccgtggccgg cgccgctcaa ggcatcccgg
      601 tcgttctccc ggggatggaa gtgcagactg cagaagacat tcatatcgtc gtgattttcc
      661 ccggcctttc tgaggccggg gattttcagg cctggctctg gaagaaaatg cctcccgtgg
      721 caaaccgtcc cgaaatattc ggagatcagc tgatcatcga caagaacaac agcatcacgg
      781 gcgagcagga ggttcttctc gtccagggag cgggctacac agccgacgaa acggcggcag
      841 aggcccggag caggggaggt cttgttatcc ttgcccacct cgaccggccg tccttctcct
      901 atgaggccgt cctcggcccg gtgccggacg atttcccctg ccatgccctg gagctttccc
      961 ccgccgtttc ccacagcaaa ttttccggat ggagagagcg gtatcccgac cgggtcttca
     1021 tccgttcttc cgattcccac aggctttcca ccatatcccg ggacaggtgt accgccatga
     1081 tgctcgaatc tccatgcttc cgggagatta aactggctct ctgcggtcag gaggggcgct
     1141 cggtgctttg cccctggggc gacggcttgt cgtggcgccg ttccagttcg gcgtagattt
     1201 ccctggggct cacccctgaa gcgagtaggg ccgcttcgag atgatagaga aggtccgccg
     1261 cctcgcaaat cgtctccttc ctgtctccgg ccgcaaccgc aagggcggtc tccacccctt
     1321 cctccccgac cttctgcgcc accctccgca gccccctcga cagaagccgg gccgtgtagc
     1381 tttcggcagg gtcgtcatga cgccgctttt caaggtatct ccagaggatt ccgggaaaag
     1441 ttccccgccc ctcgcaggga gtcgtgagaa gcctgtggaa acacgaccgc tctccggtgt
     1501 gacaggcggg acccgcaggt tccgcaaggg cgagaagagc atccccgtcg cagtccagtc
     1561 tgagttcctg gagggcgagg gtgtttccgc ttgtttcccc cttcttccac agcgttccgc
     1621 gggacctgct ccagaaggtc agcatgcccg atgaaagggt ggcttccagc gcttccctgt
     1681 tcgcccaggc catcatgagc acttcccctg aaagcacgtc ctgcacgatg acaggaacca
     1741 gaccgtcgct gccgaatttc acatcgtcca atgagaatgc cacgtcctat cacctcgacg
     1801 ggcggacggg aacgccccgt tccgcgagat atcgtttcag atcgggaatg cgtatctccc
     1861 cgaaatggaa gatggaggcc gccagggcgg cgtcgcttcc tgcccggaat gtgcccagga
     1921 tgtgctccat ggatcccgcg ccgcccgagg cgatgagggg cacgtgaacc gagccggatg
     1981 cgaggccgtt gaggtcgtcg tcatagccgg ttttcacccc gtccctgtcc atggatgtca
     2041 gcaggatttc cccgcacccc cttcggacgc cctcttcgat ccacttcagg gcgtccagcc
     2101 ccgtagcggt tctcccgccg tcgataaaga cctcccactt ttccactccg catcgcctgg
     2161 cgtccactgc caggaccaca gcctgtttcc cgagcagggc ggcgcattcc ccgatgaggg
     2221 aaggatttct tactgcggcg gagttcagcg aaattttgtc cgccccgagg gcgacaatct
     2281 cccgtgcctg ttcaaccgac gatatcccgc cgcctacggt gaaagggatg aacagggcgt
     2341 cggcaacggc cgatacccag gcggtcatgg tcctcctgcg ctccgccgag gcagtgatgt
     2401 cgaggaaaac cagctcgtcg gctccttcct ccatgtaggc cgcggccatt gcggcagggt
     2461 ccccggcatc cctgaggttc acgaagttca ctcccttgac cacccttccc tccttgacgt
     2521 caaggcaggg gataattctt ttcgtcagca tccggaagcc tccttcaggg cgtcaccgag
     2581 ttgcaccgtt ccctcgtaca gcgcccttcc cagtacggcc ccggccattc cggcgccggc
     2641 caggctgcgg atgttctcta gggacgaaat gcctccggcg gctataatgg aaagatcggg
     2701 aaacgaattc ctgaggctgc ggtacatctc aaggtcaggt cccgttcccg ttccgtccct
     2761 gtggacggcg gtaacgagaa aaagggtaaa tcccgaagcc gcaatatgct ccacaagctg
     2821 ttcgggggag aatggggccg tttcctgcca gccggaaacg gcaacctctc cgttccggat
     2881 gtccacggcg ggaataaccc tgttcccgaa cctccgaaaa aggatattcc ctccggcagg
     2941 gtcagaagcg aacaggctgc cggagtaaat tctttcggct cccgcagaaa aggctgcttc
     3001 caggtgatcc tccgtccgga gtccaccccc gtattgcact ttcagcccgg cgtccagcag
     3061 catcccgatg gcggaaagat ggcaggggaa tccttttttc gctccctcga ggtcaatgac
     3121 gtggatccat tccgccccct cttccctgaa tgaacgggcc gttcccgcga tgtcggagcc
     3181 gaacaccgtc cgttcttcga aacgaccttg ccggaggcgg acgatctcac cctggtaaag
     3241 gtccatggca ggaaaaagtt ccatcaggga acctcctttc gtatgaagtc ggccgcttct
     3301 gaaaggaggg ccagaccggc ccgtccgctc cgttccgggt ggaactggaa cccggcgatc
     3361 cgccccctga gaaccatgga ggtgaagttt gcacctccga gggaggtgcg gccggccgca
     3421 aaggcggaat cgaacagcgc gtagctgtga acgaagtaga aatattcccc tgagggaacg
     3481 gcagtcacaa atccgggggg ctgtcccgtc cattccaccg agttccagcc catgtggggg
     3541 attttttccg ctttcagaag gcggatttct cccggtatga ggccgaggcc cgggtggtcg
     3601 ccgtcttcga ggccccgttc gcagaggagc tgcatgccga gacagatccc gaggaggggc
     3661 ttaccttccg aggcccagga aataagacgg tcggcccagc cggaggctct gagccgtgcc
     3721 gtggcggggc cgaaggcgcc cactccggga aggacggcca gggtgtattc cgccgtgagc
     3781 tccgccgggg aggcgagaag ccgggcttcc tccccgaggg agctgaaggc acggcggacg
     3841 tttcccgtat tgcccacctg gtagtcgacg atggcgatca tacgagggtg ccctttgtgc
     3901 tctgaaggtt ctccgaagga aggaacgcat ctctcagggc ccgtcccgca cccttgaaga
     3961 gggcttctgc aagatggtgt gcgttgtcgc aggcgagggc ctgcgcgtgg agagttatct
     4021 ttcc
//
LOCUS       DAOTHD010000065         4022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_26488_length_4022_cov_2.591883, whole genome shotgun sequence.
ACCESSION   DAOTHD010000065 DAOTHD010000000
VERSION     DAOTHD010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26488_length_4022_cov_2.591883"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            315..1325
                     /locus_tag="P5219_01885"
     CDS             315..1325
                     /locus_tag="P5219_01885"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRX25535.1"
                     /translation="MLKKMKYAVLACLVLSALLAPAAFAEGYPSKGVTLVVPWGAGGV
                     TDVAARVFAPLFEQYLGVPVVILNRPGASGAIGTEYAYARPADGYTVVFSAETPATFR
                     IMDVSRLSFHDFEPVMLLSHSEKILAVAPESPYQTLADLVKDIRARPGKVRFSYSGAG
                     ASGHVQALLMMQKGGLDFSATPFGGGNNALLAVLGGQVDFTSPNIGTVKDYIASGKLR
                     ALAVFDRKKSAYLPDLPPITEAIPEMESFLPLDYPNCLMVKKGTPEDVTARIAEAASK
                     AVADPRWKAFLENSWYVGLDPVRGKDMLSYWDNWASVVGWVLQDAGVAKKSPAEFGIP
                     RP"
     gene            1392..1856
                     /locus_tag="P5219_01890"
     CDS             1392..1856
                     /locus_tag="P5219_01890"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRX25536.1"
                     /translation="MTNARSPWAGNLRHGVFFLAVAALFGRQGFAVHDGGQWSLSPAL
                     FPLILSGGLALLSFALIGQALLRIRAGDEGPSRKDGAPISPMRASLAFLLCVLYALAL
                     PRTGFLPSSAFFLALFCLLVGERRAARIAALSLVSPAVIYLLFRHGLNVLLP"
     gene            1869..3389
                     /locus_tag="P5219_01895"
     CDS             1869..3389
                     /locus_tag="P5219_01895"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRX25537.1"
                     /translation="MEILVSMLSQVDFVFVLLVFAGAFAGLFVGAIPGLSVTMATALL
                     VSITFTWSVKNALAVILGVFVSGVYAGAVSAILINIPGAPSSVVTTLDGYPMARKGEA
                     ALALWAATFHSFVGSVFGFLVLMFVARPVTDFALSFSAMDYFLLALFGLTTIGTLTAK
                     NYLKGGICAVLGLLLSMVGIDPFSGTARYSFGLTDLQGGIPLIAALIGLFGFAEVLDQ
                     VARGTMSAIAEKAGRMRYSFREMIRYLPLSLRSSVIGTFIGALPGAGGPVASIIAYDQ
                     ARKSVKNPTRPFGEGAVEGVIASEAANNACIGGALIPMLTLAVPGDAVTAVMLAAFYV
                     HGLQPGPLLFVRTPELFSVVLAAGFLACFAMLVLGMTVVPRLSGIVLIPKRILMPLVT
                     VLCVVGTYAVNSSMFDVGLMFCFGLAGFLMRSRGYPAAPLVLGLVLGGMMDSNLRRAV
                     NMALAGDSIFLELFGRPTTMVLSFLVVFSVLAAVPAVKERIGAAVSRMLSVFGSGR"
     gene            complement(3427..>4022)
                     /locus_tag="P5219_01900"
     CDS             complement(3427..>4022)
                     /locus_tag="P5219_01900"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRX25538.1"
                     /translation="TVLPCGTAAIVNANWVKADSEVAGDVTRGLTGGREKVLENARFF
                     RNYVPGFEKAVLSCIAPALGIREASRTECFYTLTEEDIMEGRVFPDSVCVGSWPMEYH
                     DWEQNRLVYRWSGKDYTIPFRSLLPRGMSNVLAAGRNISVTSGAYASSRVMGQCLATG
                     HAAGTAARLAIRSGSLPRELDRDLLRKTLLDEGAILE"
BASE COUNT          617 a         1280 c         1264 g          861 t
ORIGIN      
        1 ggggaaagtg gggggtgtcg atgacgtaat ggtgggagag ggaagggatt atttcaatat
       61 gaatggtgta aaaaatttgt aaatatacat tcataatatt gtagcgcttt tcttgcaaga
      121 cgttcggtgc tctccggctc agccccttat cagggtgcat ttgcaaagag gtcaacggat
      181 ggtataatcc cctctattat cccggcccgg aggccgggat tccccctgct ctccgcgctc
      241 ccgtcggaat ttgcggagcg ggcggaaaaa tttgcaggat atttgactgg aatacaatga
      301 agggacgtgg tgtgatgctg aaaaaaatga agtatgccgt tcttgcctgc ctggttctct
      361 ctgccctcct tgcccctgcc gcctttgcgg aagggtaccc gtccaagggc gtgaccctcg
      421 tggtgccctg gggtgcgggc ggcgtgaccg acgtggccgc cagggtgttc gcccccctgt
      481 tcgagcagta cctgggagtc cccgtggtga tcctcaaccg ccccggggcc tcgggagcca
      541 tcggcaccga gtacgcctac gccaggcccg ccgacggcta cacggtcgtt ttttcggcgg
      601 agacgccggc cacgttccgc atcatggacg tgagcaggct ttcgttccac gatttcgagc
      661 ccgtcatgct gctctcccat tccgagaaga ttctcgccgt ggccccagaa tctccttacc
      721 agaccctcgc ggacctggtg aaggacatcc gtgcccggcc cggcaaggtg cggttttcct
      781 acagcggcgc cggggcaagc ggccatgtcc aggccctcct gatgatgcag aagggcggcc
      841 ttgacttctc cgcaacgccc ttcggcggcg gcaacaacgc cctcctggcg gtgctcgggg
      901 ggcaggtgga cttcacgtcc cccaacatcg ggacggtgaa ggactatatc gcctccggga
      961 agctccgggc cctggccgtc ttcgacagaa agaagagcgc ctacctgccc gaccttcctc
     1021 ccatcaccga ggccatcccg gaaatggaat cctttctgcc cctggattat cccaactgcc
     1081 tcatggtgaa gaaggggacc cctgaagacg tgacggccag gatcgccgaa gcggcgtcga
     1141 aggccgtggc cgaccccagg tggaaggcgt tcctcgagaa cagctggtac gtggggctcg
     1201 atccggtgag ggggaaagac atgctctcct actgggacaa ctgggcttcc gtggtcggat
     1261 gggtgctcca ggacgccggg gtggcgaaga agagccccgc ggaattcggc atccccaggc
     1321 cgtagcgtca aaggttcgct tcggggtccc ttcggaagga ggggcccctg ttccatgaaa
     1381 ggaaggaaaa gatgacgaat gcccgctctc cctgggccgg aaatctccgg cacggggtct
     1441 tttttctcgc cgttgccgcc cttttcggcc ggcaggggtt tgccgtccac gacggcggac
     1501 agtggagcct ttcaccggcc ctcttccccc tgatcctgtc aggggggctt gccctgctct
     1561 ccttcgcgct catcgggcag gccctcctca ggatccgcgc aggggatgag ggcccttccc
     1621 ggaaggatgg agcaccaatt tcacccatgc gggcatccct ggcctttctc ctgtgtgttc
     1681 tgtacgccct ggcgctgccc cggacgggat ttctgccctc gagcgcgttt ttcctcgccc
     1741 tgttctgcct gctggtcggg gagcggcgag ccgctaggat tgccgccctg tccctcgttt
     1801 ccccggcggt gatttacctg ctgttccgtc acgggctgaa cgtgcttctt ccctaggggg
     1861 ccgggccgat ggaaatcctt gtctccatgc tgtcccaggt ggacttcgtc tttgttctgc
     1921 tcgtcttcgc gggtgccttc gccgggctct ttgtcggcgc cattcccgga ctctccgtca
     1981 ccatggccac ggcgctgctg gtctcaataa cctttacctg gtcggtgaaa aacgccctgg
     2041 ccgtcattct cggagtgttc gtgtcggggg tttacgccgg ggcggtttcc gccatcctga
     2101 tcaacattcc cggcgctccc tcgtcggtgg tcaccactct cgacggctac cccatggccc
     2161 ggaagggaga ggcggccctg gccctgtggg cggccacgtt tcactccttt gtcgggagcg
     2221 tgttcggctt cctggtgctc atgttcgtcg cccgccccgt gactgatttc gccctctcct
     2281 tttccgccat ggactatttt ctcctggccc ttttcggtct caccaccatc gggacgctga
     2341 cggcgaagaa ttacctcaag ggaggcatct gcgcggtcct tggactgctg ctgagcatgg
     2401 tgggcatcga tcccttctca ggcacggccc gctattcctt cggcctgacc gacctgcagg
     2461 gaggcattcc cctcattgcc gccctgatcg gcctgttcgg tttcgccgag gttctcgacc
     2521 aggtggcgag ggggaccatg agcgccattg ccgaaaaagc gggcaggatg cggtattcct
     2581 tccgggagat gatccggtac ctgcccctgt cgctccggtc gtcggtcatc gggaccttca
     2641 tcggtgccct tcccggggcg ggaggacccg tggcctccat catcgcctac gaccaggcca
     2701 ggaagtcggt gaagaatccc acccggccct tcggggaagg tgctgtggag ggggtcatcg
     2761 ccagcgaagc ggccaacaac gcctgtatcg gcggagccct cattcccatg cttacccttg
     2821 ccgttccggg tgacgccgtc acggcggtca tgctggcggc attctacgtc cacggcctcc
     2881 agcccgggcc ccttctgttc gtccggacgc cggaactgtt ttccgtggtg ctcgccgccg
     2941 gctttctcgc ctgcttcgcc atgctcgtgc tcggcatgac ggtggttccc cgcctctccg
     3001 gaatcgtcct cattccgaaa aggatcctca tgcccctggt gacggtgctc tgcgtggtgg
     3061 gaacctacgc cgtgaactcg agcatgttcg acgtggggct catgttctgc ttcggcctcg
     3121 cgggatttct gatgaggagc aggggatacc cggcagcacc gctggtgctc ggccttgtcc
     3181 tcggcggcat gatggactcg aacctccggc gggctgtgaa catggccctg gccggagaca
     3241 gcattttcct ggagctgttc ggccgcccga cgaccatggt gctttccttc ctggtggtct
     3301 tctcggtcct cgccgccgtt ccggcggtga aggaacggat cggagcggcg gtctcccgta
     3361 tgctctcggt cttcggatcg ggcagatgac ggaaactaac cagggccgcc gcggggcggc
     3421 cctgctttat tccaggatgg ccccttcgtc cagcagtgtt ttccggagaa gatcccggtc
     3481 cagctcccgg gggaggctgc cggagcggat ggcgagccgt gctgccgtac cggcggcgtg
     3541 accggtggcg aggcactgtc ccatgaccct tgaagacgca taggctccgg aggtgacgga
     3601 aatgttcctc ccggcggcga ggacgttgct cattccccgg gggaggaggg aacggaaggg
     3661 tatggtgtag tcctttccgc tccaccggta gacgaggcgg ttttgttccc agtcgtggta
     3721 ctccatgggc caggagccga cgcagacgct gtcgggaaag acccgcccct ccatgatgtc
     3781 ctcttcggtc agggtgtaaa agcattctgt ccgggaggct tccctgatcc cgagggcggg
     3841 agctatgcag gacagaacgg ccttctcgaa tcccgggacg taattccgga agaaccgggc
     3901 gttttccagg accttctccc gtcccccggt cagcccccgg gtgacgtccc ccgcaacttc
     3961 ggaatcggcc ttcacccagt tggcgttcac gatggcggcg gtgccgcacg ggaggaccgt
     4021 aa
//
LOCUS       DAOTHD010000066         4008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_26612_length_4008_cov_2.845940, whole genome shotgun sequence.
ACCESSION   DAOTHD010000066 DAOTHD010000000
VERSION     DAOTHD010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26612_length_4008_cov_2.845940"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1487
                     /locus_tag="P5219_01905"
     CDS             <1..1487
                     /locus_tag="P5219_01905"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRX25539.1"
                     /translation="GFYRQVSLGARGTVLLVDSEGTILAGTVDGGETPFGASIVNSGL
                     LTAMKAGEAGALECAGLPDGILRLFSYRKVSPYPLYVVAGREKNGVFAEYRRRRAAGL
                     FSLVVFNAFVLVFFGASVRALKKERQAEERFRNFFEQNSVGFALVAADSAWIHFNGKL
                     CEMLGYSREELMNLPWKKLTPPDVLERELPIFEKALWSGTSDFFIEKQYVRKDGVRID
                     VDVTTKIMKNADGSVRYFASIIQDVSERKKGERLLEKRAAELEKHREAIIDGMAVLAE
                     YRDMGTGNHVRRTKQYVKLLLERSGSASLFPEKDLPMIWQCAVLHDIGKVGVPDAVLL
                     KPGGLTPEEFGVIRRHPSIGSDVLLRAEELLGSDSFITYARQITEYHHERWDGTGYPH
                     GLAGEDIPVLARITAIADVYDALISRRPYKEPLPHEAAVEQIRAGSGTLFDPRLVEVF
                     LGCAGEFDEIAKNNADEEPPGTEETESGNVPGEGGTGRRGRDPS"
     gene            1526..2461
                     /locus_tag="P5219_01910"
     CDS             1526..2461
                     /locus_tag="P5219_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRX25540.1"
                     /translation="MGSGAARGMAHIGVLQALEGAGIRPDIIAGTSIGALVGGVYAAG
                     KLDELEEVSLGLDWKKAAGYFLEVSFPRSGLIEGTKVTDFLSEIVGSAKIGALPVRYA
                     SVAADVMTGNEVVISDGDLVSAIRASISVPGIFTPARRNGSFLVDGGLVNPVPVSVCR
                     AMGADVVIAVDLNNGRVKEAPQAGLPEKTSPEGGPGRSGVAAVEIFAWLEKKTSGFDV
                     RFLEPVKEWMSRDPAPGIFDVLGNSLRIMEEQIAAARLEIDRPDMLIRPAVGNVRFLE
                     FHLAGEMIAEGFRSAKEVLAGGAVPEQGRGGASEI"
     gene            2509..3612
                     /locus_tag="P5219_01915"
     CDS             2509..3612
                     /locus_tag="P5219_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25541.1"
                     /translation="MAAQPKGPWFQRFLINFFTVVLAVLVFWVLGFLMEDIESIRGPQ
                     YRDIEARYVDRGLVEKSKQLAEEIAGAGRAIEAKREEMRILSDSSSNLQGTINQLIEL
                     QKLTIQKSLSLPEAHSANLSESLAGFLESRKRYQAYIAELSALSERKRILESELAGIE
                     KTIEEQRKPARKEYERLDEKHNLRLAAFQLAVLLPLLAAGGYLAARKRGSVYFPLFLA
                     FGGAVLVKSALVIHRYFPTRYFKYVIVLGLLAAVVRILVTIIRIVAFPRAQWLTKQYR
                     EAYERFLCPVCEYPIRTGPRKFMFWTRRTVNRVPLSNGGEEEKPYTCPACGTRLFEEC
                     EVCGRMRHSLLPFCLHCGAEKKIAGDAEKGKSQ"
     gene            complement(3683..>4008)
                     /locus_tag="P5219_01920"
     CDS             complement(3683..>4008)
                     /locus_tag="P5219_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25542.1"
                     /translation="QEFLSPFEIEKAEVYRLKGNTMAAERAGRAAWGYFLEGRPFDDL
                     FDNRDEKAMYCTELIWKACETAGVDLFGGKRSSYFSPVPFYGNVLFPSALIRSPLLEK
                     VMTLD"
BASE COUNT          804 a         1115 c         1349 g          740 t
ORIGIN      
        1 ccggctttta ccgccaggtc agtctcggtg cccggggaac cgtcctcctc gtggattctg
       61 agggaacgat tctcgcaggg actgtggatg gaggagaaac ccccttcggg gcctccatcg
      121 tgaacagcgg gctcttgacg gcaatgaagg ccggagaggc gggggcgctg gagtgcgcgg
      181 ggctgccgga cggcatcctg aggcttttca gctacaggaa ggtgtctccg taccctctgt
      241 atgtcgtcgc cggaagggag aagaacggcg ttttcgcgga ataccgcaga aggagggcgg
      301 cgggtctctt cagccttgtg gtgttcaatg ctttcgttct tgtgttcttc ggggcctcgg
      361 ttcgggccct gaagaaagag cggcaggcgg aggagcggtt ccggaacttc ttcgagcaga
      421 acagcgtcgg gttcgccctt gtcgcagcag acagcgcctg gattcatttc aacgggaagc
      481 tctgcgagat gcttgggtac agccgggaag agctgatgaa cctcccatgg aaaaaactga
      541 cgccgccgga cgtcctggaa agggaactcc ccatttttga aaaggcttta tggagcggca
      601 ccagcgactt tttcattgaa aagcagtacg tcaggaaaga cggcgtcagg atcgacgtgg
      661 atgtaaccac aaagatcatg aagaatgccg acggttcggt acgctatttc gcgtccatca
      721 tccaggatgt ctcggaacgg aagaagggcg aacggctcct tgagaagcgg gcggcggagc
      781 tggagaagca ccgggaggcc atcatcgacg gcatggccgt cctggcggaa taccgggata
      841 tgggaacagg caaccatgtc cgcaggacga agcagtatgt gaagctgctc ctcgagcggt
      901 cggggagcgc gtccctcttt ccggagaagg acctgcccat gatctggcag tgcgcggtcc
      961 tccacgatat cggaaaggtg ggggtgccgg acgccgtgct gctgaagccg ggcgggctga
     1021 caccggagga attcggcgtc atccggcggc acccgtccat cggcagcgac gtgctgctcc
     1081 gggcggagga actcctgggc agcgattcct tcatcaccta cgcgcgacag atcacggagt
     1141 accaccacga gcggtgggat ggcacggggt acccccacgg cctggccggg gaggacattc
     1201 ccgttcttgc ccgcatcacc gccatcgccg atgtctacga cgccctcatc tcgagaaggc
     1261 cctacaagga gcctcttccc cacgaagcgg cggtggagca gatccgggcc ggttccggaa
     1321 ccctgttcga tccccggctc gtggaggtgt ttctcggctg tgccggggaa tttgacgaaa
     1381 tagcgaaaaa caatgccgat gaagagccgc ccgggacaga ggaaacagaa tccgggaatg
     1441 tccccggaga gggcggaacg gggcggcggg ggagggatcc atcctgaccg gaagcagcgg
     1501 cggagagcgg aaaattgggc ttgccctggg gagcggcgcg gcccggggga tggcccatat
     1561 aggggtgctc caggcccttg aaggggcggg gatacggccg gatatcatcg cggggacgag
     1621 catcggcgcc ctggtgggag gagtgtacgc cgccgggaag ctcgacgaac tggaggaggt
     1681 ctccctcggt ctggactgga aaaaggccgc cgggtatttt ctggaggtct cttttccccg
     1741 gtcggggctc atcgagggga cgaaggtgac ggactttctc tcggaaatcg tgggttccgc
     1801 gaagatcggc gcccttccgg tgcggtacgc ttcggtggcg gcggacgtga tgaccggaaa
     1861 cgaggtggtg atcagcgacg gagaccttgt ctccgccatc cgggccagta tctccgtgcc
     1921 cggcattttc accccggcac ggcggaacgg gagcttcctc gttgacggag ggctggtgaa
     1981 tccagtgcct gtcagcgtct gccgggcaat gggcgcggac gtggtcatcg ccgtggatct
     2041 gaacaacggc cgggtgaagg aggcccccca ggccggactt ccggagaaga cttcaccgga
     2101 aggcggtccg ggacggagcg gagttgccgc ggtggagatt ttcgcatggc ttgagaagaa
     2161 gacaagcggt tttgatgtcc gttttctcga gccggtgaag gaatggatgt cccgggatcc
     2221 tgctccgggt atttttgacg tgctcggcaa ttcgctccgc atcatggagg agcagatcgc
     2281 cgcggcgcgg ctggaaatcg accggccgga catgctgatc cgcccggcgg tggggaatgt
     2341 ccgctttctc gagtttcatc tcgccgggga gatgatcgcc gaaggcttcc gttcggcgaa
     2401 agaggttctt gccgggggag cggtcccgga acagggaagg ggcggggctt cggagatctg
     2461 aagctccgga agggaaggta cggaacgggc acaagggagg gaatggccat ggcggcacaa
     2521 ccgaaaggtc cctggttcca gaggttcctg atcaactttt tcaccgttgt cctggcggtg
     2581 cttgtcttct gggttctcgg ctttctcatg gaagatatcg aatccatccg gggaccgcag
     2641 tacagggata tcgaggcccg gtacgtggac agggggcttg tggaaaagtc gaaacagctc
     2701 gcggaagaga tcgccggtgc cggcagggcg atagaagcca agcgggaaga gatgaggatt
     2761 ctcagcgaca gttcgtcaaa cctccagggg accatcaacc agctgatcga actgcagaaa
     2821 ctcaccatcc agaagtccct ctccctgccc gaggcccatt cagcaaacct ttccgagagt
     2881 ctcgccggtt ttcttgaaag ccggaaacgc taccaggcct acatcgccga actttcggcc
     2941 ctgtctgagc ggaaaaggat cctcgaatcg gaactggccg ggatcgagaa gaccatcgaa
     3001 gagcagagga aacccgcccg gaaagagtac gaacggctgg acgagaagca taatctcagg
     3061 ctggccgcct tccagctggc ggtcctgctc cccctgctgg ccgccggggg gtaccttgcc
     3121 gccaggaaac gagggagcgt ctacttcccc ctttttctcg ccttcggcgg cgcggtgctt
     3181 gtgaaatcgg ccctcgtgat ccacaggtat ttcccgacgc gctacttcaa gtatgtcatt
     3241 gtcctcgggc ttctcgcggc ggtcgtcagg atcctggtca ccatcatcag gatcgtggcc
     3301 ttccccaggg cccagtggct tacgaagcag taccgggagg cttacgagcg gttcctgtgc
     3361 ccggtctgcg agtatcccat ccggacaggc ccgaggaagt tcatgttctg gacacggcgc
     3421 acggtgaaca gggttcccct gtcgaacggc ggagaggaag agaagcccta tacctgcccg
     3481 gcctgcggga cccggctgtt cgaggaatgc gaagtctgcg gccgaatgcg gcactctctt
     3541 cttcccttct gcctccactg cggggcggag aaaaaaatcg ccggggacgc cgaaaagggc
     3601 aagagccaat aaacacaccg gaagggctgg gttgtccggg ggagagggaa cgccggaaag
     3661 cggacgtccc ggaggagagc cttcagtcca gggtcatcac tttttccaga agaggactgc
     3721 ggatgagggc ggaggggaac agcacgttcc cgtagaaggg gacgggggaa aagtaggaag
     3781 acctttttcc gccgaagaga tcgacacctg ccgtttcgca ggctttccag atgagttccg
     3841 tgcagtacat cgctttctcg tcccggttat cgaagagatc atcgaagggc cttccctcga
     3901 ggaaatatcc ccaggctgcc ctgcctgccc tttctgccgc cattgtattc cctttcaggc
     3961 ggtacacctc ggccttttct atctcgaagg gcgacagaaa ttcctgca
//
LOCUS       DAOTHD010000067         4000 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_26666_length_4000_cov_3.320152, whole genome shotgun sequence.
ACCESSION   DAOTHD010000067 DAOTHD010000000
VERSION     DAOTHD010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26666_length_4000_cov_3.320152"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1552)
                     /locus_tag="P5219_01925"
     CDS             complement(<1..1552)
                     /locus_tag="P5219_01925"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HRX25543.1"
                     /translation="MSTLEFYKDPDSFSSIKSQARTTIETAFYGNNVVPVANLREAYD
                     LARTSPGTVELTGMPVYRPEGQGLPSGSNVLLMNDGSVVGRCAAARKIVGEPGVNMGE
                     LAPVLREAVYGSRFRTFYAAEAYIGLEKDFMVKAHLLAPKNHENLVYNWMLNFQYLNK
                     TYIDMYRESRALPDGDIFMFADPDWTSPEYPLGLAFFDPVHNCAAVLGMRYFGELKKG
                     TLTLAWGTAVRNGFASCHGGLKRYNLKSGRSFVSAVFGLSGSGKSTLTHARHGGKYDI
                     TVLHDDAFIINVKEKYAIALEPSYFDKVQDYPMGCEDNKFIISLQNCGVTRGEDGLLY
                     PVTEDLRNGNGRAIKSKLWSPLRVDRIDEPINAVFWLMKDQSLPPVLRLKGPDLAAVM
                     GATLATKRTTAERLAPGIDPNALVIECYANPFRTYPLALDYERFRTLFRDGVDCYILN
                     TGDFMGKKVTPADTLGILEAIIEEKARFVPFGPFSDMETLELERFPVDFSDEKYRREC
                     FARCWDRLA"
     gene            complement(1712..2089)
                     /locus_tag="P5219_01930"
     CDS             complement(1712..2089)
                     /locus_tag="P5219_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRX25544.1"
                     /translation="MSLLLRLLLKAAPLLLFLLLSRAVRDLFRSYQAGERAYDRPPHG
                     GSGPGASAAGRNSRRDPYSVLGCSPSATDGEIKKRYRELLGKYHPDKFIGQDLDADFV
                     DLASKKFQEIQEAYDAIRSRRGF"
     gene            complement(2121..3395)
                     /locus_tag="P5219_01935"
     CDS             complement(2121..3395)
                     /locus_tag="P5219_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25545.1"
                     /translation="MGHPISPLSGRGGTAGRIPAEREEKGSRSPARGAAGTLFLLFAC
                     AVVLYLLFHPHPGLSPSGALQIAKSSRDLSPLLSNEEFIKTYEDAANAPETIGWLVDR
                     TRAGGFLVSYIYLDGKGDFKGWFFEVPAGSGVARRVTRSMGEHYAALIDDRFERSTPD
                     VSEKDRVTRWVKEAKAPSGDGTVMEAISREVGRTGVRKEYGWMAHPLGNGKWLVGFVY
                     EPAGAGDGEKRGWVFQVNTREIPPLPGRGLPLGTGSVDDLLRLFGGMGEAPLPPLSIP
                     ADGTDCIPLRSFLESTLRRFGGSPSEARDRLGEPGAVRRRSVRQPSHPVEGKPVLTMM
                     WPGLSLDFSGVPGSERLVAAVVKGGDHPFGPDPGIRVGDPFVRVSELLGQPDGKSSQS
                     MVYTGRDVSCDLIFNLRKDGRIAGMFFSVAAE"
     gene            3537..>4000
                     /locus_tag="P5219_01940"
     CDS             3537..>4000
                     /locus_tag="P5219_01940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HRX25546.1"
                     /translation="MERKRRGRAMNPVSIGGYIQTSFLDYPGSAAAVVFFRGCPFRCP
                     FCHNPELVIPGMGGDSFEWRDILGDLERRKNFLDGVCITGGEPTIQEGLISFLGDLRS
                     LGLKIKIDTNGARPGVLEHILDRNLADYAAMDIKASREKYPLASGWNGDLSLV"
BASE COUNT          787 a         1222 c         1198 g          793 t
ORIGIN      
        1 aagcgagacg gtcccagcac cgggcgaagc actcccgtct gtacttttcg tccgaaaaat
       61 ccacgggaaa acgctcgagc tcgagggtct ccatgtcgct gaagggaccg aagggaacga
      121 aacgggcctt ctcctcgatg atggcctcga ggatccccag ggtgtcggcg ggagtgacct
      181 tctttcccat gaaatctccc gtgttgagga tatagcagtc caccccgtcc cggaagaggg
      241 tccggaaccg ttcgtaatcg agggcgaggg gataggtccg gaaggggttg gcatagcact
      301 cgatcaccag cgcattgggg tcgatgcccg gggcgagccg ctcggcggtg gtccgcttgg
      361 tggccagggt ggcccccatg acggcggcga ggtcgggtcc cttgagccga aggacaggcg
      421 gcagggactg gtccttcata agccagaaga cggcgttgat gggctcgtcg atgcggtcca
      481 cccgcaaggg cgaccagagc ttcgacttga tggccctgcc gtttccgttc cgcaggtcct
      541 cggtcacggg atagagcagc ccgtcctctc ccctggtcac gccgcagttc tgcagggaga
      601 tgatgaactt gttgtcctcg caccccatgg gataatcctg aaccttgtcg aagtaggaag
      661 gctccagggc tatagcatac ttttccttca cgttgatgat gaaggcgtca tcgtggagga
      721 cggtgatatc atacttcccc ccgtggcggg cgtgggtcag ggtggacttt cccgagccgg
      781 agaggccgaa cacggcggag acgaaggacc gtccgctttt caagttgtac cgcttcagtc
      841 ccccgtggca ggatgcaaag ccgttccgca cggcggtgcc ccaggcgagg gtgagggttc
      901 cctttttcag ctcgccgaaa tagcgcatgc cgaggacggc ggcacagttg tggacgggat
      961 cgaaaaaggc aaggccgagg gggtactcgg ggctcgtcca gtcgggatcg gcgaacatga
     1021 agatgtcgcc gtcgggaagc gcccgggatt ccctgtacat gtcgatatag gttttgttca
     1081 ggtactggaa gttcagcatc cagttgtaca ccaggttctc gtggtttttc ggggcgagga
     1141 gatgggcctt gaccatgaaa tctttttcga ggccgatata ggcctcggcc gcgtaaaagg
     1201 tgcggaaccg gcttccatac accgcctccc tgagaacggg cgcaagttct cccatattca
     1261 cccccggttc ccccacaatc ttccgtgcgg cggcgcaccg gcccaccacg gaaccgtcgt
     1321 tcatcagcag gacgttgctc ccggaaggca gtccctgccc ttcgggacgg tacaccggca
     1381 tgcccgtaag ctccactgtt ccgggactgg tccgggcaag atcgtacgct tcgcggagat
     1441 tggcgacggg aaccacgttg tttccgtaaa acgcggtctc aatggtggtt cgcgcctggg
     1501 actttatgga agagaagcta tcgggatcct tgtagaactc cagagttgac atggcgactc
     1561 tccttctccc tcgggccggg gacggaaatt tttgtccggc accgtcgccg gacggggtca
     1621 ttgtaccatg ggccgtacca ctgaacgaaa caaaaaagaa gcctgctaca agattattat
     1681 gaaaaatacc ctccagatgc gaacaatatg atcaaaagcc tcgcctcgac cgtattgcat
     1741 cgtaggcttc ctggatctcc tggaactttt ttgacgccag atccacaaag tcggcgtcca
     1801 gatcctggcc gatgaacttg tcgggatgat atttcccgag aagctcccgg tacctttttt
     1861 ttatctctcc gtccgtcgcg gagggtgagc agccaaggac gctgtagggg tccctccggg
     1921 aattccggcc ggcagcggat gcacccggcc ccgatcctcc gtgggggggc cggtcatagg
     1981 ctctttcccc cgcctggtag gaccggaaga ggtcccggac agcccgggac aggagaagga
     2041 acagaaggag cggcgccgcc ttcagcagca gcctcagcaa cagggacatg ggaacctcct
     2101 tgaaattgtc cgtcttcggc tcattccgcc gcgacggaga agaacattcc ggcgatccgg
     2161 ccgtccttcc tcaggttgaa aatgagatcg caggaaacgt cccggccggt atagaccatc
     2221 gattgtgaac ttttgccgtc cggctgtccg agaagttccg acaccctgac aaagggatcc
     2281 cccacgcgaa ttcccggatc aggaccgaaa gggtgatccc cccccttcac cacggcagcc
     2341 accaggcgct cgctgccggg caccccggaa aagtccaggg aaagtccggg ccacatcatg
     2401 gtgagcaccg gctttccctc cacgggatgt gaaggctgcc tgacggacct gcgcctcact
     2461 gccccaggct cgcccaaccg atcccttgcc tccgaaggag aaccgccgaa gcgccggagg
     2521 gtggactcga gaaaagaccg gagagggata caatccgttc cgtcagccgg tatggacagc
     2581 ggaggcaggg gcgcctcccc catgcctccg aaaaggcgga ggagatcatc caccgatccc
     2641 gttccaaggg gaagccctct cccgggaaga ggaggtatct ccctggtgtt gacctgaaag
     2701 acccaccctc tcttttcgcc gtctcccgcc cctgccggtt cgtacacgaa gccgaccagc
     2761 cattttccgt ttccgagagg gtgggccatc cagccgtact ccttcctgac cccggtcctg
     2821 ccgacctccc tggaaatcgc ctccataacg gtgccgtcgc cggatggggc ctttgcttcc
     2881 ttcacccacc gggtgaccct gtccttttcg gaaacatccg gagtgcttct ctcgaaccgg
     2941 tcgtcaatca gggcggcgta atgctccccc atggaccggg tgacccttct tgccacgccg
     3001 ctccctgccg ggacctcgaa aaaccatccc ttgaaatcgc ccttcccgtc gagatagatg
     3061 taggatacaa ggaagccgcc cgcccgggtc cggtccacca gccaccctat agtttccgga
     3121 gcgttggccg catcctcgta ggtcttgatg aattcctcgt tgctcagaag aggagaaaga
     3181 tccctggagc ttttggcgat ctgcagcgcc ccggacggag agaggcccgg atggggatga
     3241 aagaggagat acaggaccac ggcgcaggcg aacaaaagaa aaagagtccc ggcagcacct
     3301 cttgcggggc ttctggagcc tttctcttct ctttccgccg gaatcctccc ggcggtgccg
     3361 cctcttcccg aaaggggact gatcgggtgc cccattgccg tttctcctct ccgtgctctc
     3421 tctgccgccg caccgggcgg gggaatcgtt acaggccgac gcttccgcct gtaacgttat
     3481 attataatac tttttctttc cggcggagat ttctctcttc ccgaaggggt ggtatcatgg
     3541 agcggaaaag gaggggaagg gccatgaacc ctgtctccat aggaggatac atccagacga
     3601 gttttctcga ttatcccggg tcggccgcgg cggtggtttt tttcaggggc tgccctttcc
     3661 gctgcccgtt ctgccacaac ccggaactgg tcatccccgg gatgggaggc gattccttcg
     3721 aatggcggga catcctcggc gaccttgaga ggcggaagaa ctttctcgac ggcgtctgca
     3781 tcaccggcgg ggagccgacg atccaggagg ggctcatttc ctttctcggg gatcttcgtt
     3841 ccctcgggct gaagatcaag atcgacacca acggcgcccg ccccggtgtg ctggagcaca
     3901 tccttgaccg gaatctggca gactacgccg caatggacat caaagcctcc agggagaaat
     3961 accccctggc ctcgggatgg aacggagatc tttcccttgt
//
LOCUS       DAOTHD010000068         4000 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_26667_length_4000_cov_3.221800, whole genome shotgun sequence.
ACCESSION   DAOTHD010000068 DAOTHD010000000
VERSION     DAOTHD010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26667_length_4000_cov_3.221800"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..702
                     /locus_tag="P5219_01945"
     CDS             148..702
                     /locus_tag="P5219_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRX25547.1"
                     /translation="MKRPVLILGDPLLRRKCAPVEDFSDPLLNEEMEDLREALELFRK
                     EHGFGRGIAAPQIGIPKRLIALNLGKGSFVIANPEVISRSPETFTLWDDCMSFPDLMV
                     RVRRNRSVSIRYRDEKGAPQEWTDIGIAESELLQHEMDHLEGVLAVDRAIGTGDIVYM
                     AEYRRHRELYDGRCDYSIVPTIGG"
     gene            751..1305
                     /locus_tag="P5219_01950"
     CDS             751..1305
                     /locus_tag="P5219_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRX25548.1"
                     /translation="MKSTDELRNEHRGIEVMLDILDGAAEKLSGGAPADFGHLDGMME
                     FLSVFADTCHHGKEEDFLFPGLEAAGIPNEGGPIGVMLGEHRKGREHIAGMKRGITAM
                     KAGDRGGVSMFAENAAGYTALLRQHIQKEDNVLFVMAEQRLDAAKDEELFREFEKLEE
                     ERIGKGKHDEFHALLDRLQEIYTR"
     gene            1486..1653
                     /locus_tag="P5219_01955"
     CDS             1486..1653
                     /locus_tag="P5219_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25549.1"
                     /translation="MDIHLKADEVEEMKSLMAEDGWTGSVKDYARELFLEGMSYHKAR
                     QAGGYLHPEEK"
     gene            1879..3420
                     /locus_tag="P5219_01960"
     CDS             1879..3420
                     /locus_tag="P5219_01960"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRX25550.1"
                     /translation="MDVFEFYSFFLFGVFAAYLALFFMAVKYSDRTFASVFSLICLAS
                     AVYVLGAALQFNSKSVEEILFFQKLKYFGAALVPGLWIVFAYRVHSGKKMSLAMLLCV
                     FSIPALVLFLVSTNEYHGLFYSGLKVFQNGEYLFSRRATGPLYVLNVAYAYFSLFYCL
                     YFFSLVWIKSRFRLNSPYFLLLAGQLLSAALFGLYLAGMTPLWVDMLPVGYLVRAVFC
                     AVAIFKYDVLEIKEIYNKEIFSEIKEGLMLVSCDGLLIDYNRSARTVFEWLNEKNRGA
                     PIHSLDSRFNVSTGAKNFDIGVGRGDEERIYEFRLTELKEGRKTAAFLYIFLDVTDKQ
                     RLIEKLRYMADHDSLTGLFNRGRILVEANRLLSARKLEGRIFSICMIDADHFKNLNDS
                     CGHIAGNRMLCELAAVFRRIAGERGIIGRYGGEEFLVLLPDAGAKEAAETAEKLRAGI
                     EAATVDFDGKTLQVTISIGVTSAGLHPFEEETIHSLVSRADRALYRAKNSGRNRVCVF
                     GEDDL"
     gene            complement(3548..>4000)
                     /locus_tag="P5219_01965"
     CDS             complement(3548..>4000)
                     /locus_tag="P5219_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRX25551.1"
                     /translation="IILDLGGRYKSYCSDTTRTFFTGTPTGEQRKVYEIVRQAQAAGE
                     AAVKPGATGQDVDRAARQVIIDAGYGEYFFNRVGHGIGLAVHESPYMIEGNDRPLEPG
                     NVFSVEPGIYLPGKFGVRIENIVAVRADGTGEALNHFPRDLDSVCIRG"
BASE COUNT          862 a         1049 c         1216 g          873 t
ORIGIN      
        1 agcgaagatc gccgtatcca cagtgcggga ggaactcgtc aaatacccgg ggatagaaga
       61 agtccttttc tgctgtttct cggaagggga ccgcgcggtc tacgaagaac tgcttgagta
      121 attttttcaa attatggagg cgttatgatg aaaagaccgg tcctgatact cggcgatcct
      181 cttctgcggc ggaaatgcgc ccctgtggag gatttttccg atcccctgct gaacgaggag
      241 atggaggatc tcagggaggc cctggaactg ttccggaagg aacacggttt cggccggggg
      301 atcgccgcgc cccagatagg catcccgaag cggctcattg ccctgaacct tgggaaggga
      361 tccttcgtca tagcgaaccc ggaggtcatc tccaggagcc cggaaacttt caccctctgg
      421 gatgactgca tgtccttccc ggacctgatg gtgcgggtcc gccggaaccg gtccgtcagc
      481 atacggtacc gggacgaaaa gggcgccccg caggaatgga cggacatcgg gattgccgaa
      541 tcggagctgc tgcagcacga aatggatcac ctggagggcg tgcttgccgt tgacagggcc
      601 atcggaacgg gggatattgt ctacatggcg gagtaccgga ggcacaggga actctacgac
      661 ggccggtgcg actattccat tgtccccacc atagggggat gaacctttcc ccggggaaca
      721 gccgtctgac ccgcgaaagg agagagtgaa atgaagtcga ctgacgaact gagaaacgag
      781 cacaggggca tcgaggtcat gctggacatc ctcgacggtg cggcggagaa gctgagcggc
      841 ggagcgcccg ccgacttcgg ccatcttgac gggatgatgg agttcctttc cgtcttcgcc
      901 gacacgtgtc atcacggcaa ggaggaggat ttcctctttc cgggccttga agccgcgggt
      961 atccccaacg agggaggccc catcggcgtg atgctcggcg aacaccggaa ggggcgggag
     1021 cacatcgccg gcatgaagcg gggcatcacc gccatgaagg caggcgacag gggaggagtc
     1081 tccatgttcg cggagaatgc cgccgggtac acggccctgc tgcggcagca catccagaag
     1141 gaggacaacg tcctcttcgt catggccgaa cagcgcctcg atgccgccaa agacgaagaa
     1201 ctcttccggg agtttgaaaa acttgaggag gagcggatcg ggaagggaaa gcacgatgaa
     1261 ttccacgctc ttctggaccg cctgcaggag atctacaccc gctgaagcgg gacgggagat
     1321 accgggaaaa gccggggaaa ccacagtttt tcccacgcgg cttccagagg cgccctggcg
     1381 cagttttcag gccgaatggt tcatctattt ggaattgaca acacactttc cccttgttaa
     1441 gatgacggcg ggatgcccca ggccgaaatc aggggaggtg cagtgatgga tattcatctg
     1501 aaggcggacg aggtcgaaga aatgaaaagc ctcatggcgg aagacgggtg gacgggatcc
     1561 gtgaaggact acgccaggga gctgtttttg gaaggcatgt cctaccacaa ggcgcgacag
     1621 gccgggggat acctgcaccc ggaggaaaag tgaagggagt gccggaaaaa cggaaaggcc
     1681 cggggcctgc tgacaggccc cgggcctttt tcgggtattc gatccgcgga tttgccccag
     1741 ccagtcccct gaaaaagcac gatgccgtac ggaaaaaatt acatttgccc tgtaggcatt
     1801 gtttatttat gaaaaatcag agaaaatcga gaaagaaaaa cggcattggt ccgatacctt
     1861 ttctggggga gggcccggat ggacgtcttc gagttctatt ctttctttct ttttggggtt
     1921 tttgccgcat atcttgcctt gttcttcatg gccgtcaaat attcggacag gacgtttgct
     1981 tccgttttct ccctgatttg cctcgcgagt gcggtgtatg tcctcggtgc ggcgctccag
     2041 ttcaattcaa aaagcgtgga agaaatcctc tttttccaga aactcaaata cttcggggcc
     2101 gcccttgtcc ccggattgtg gatcgtcttt gcctatagag ttcattccgg caaaaaaatg
     2161 agcctggcga tgcttctgtg tgtattttcg atcccggcac tggtgctgtt tctggtgtca
     2221 accaacgaat accacggtct gttttattcg ggtttaaagg tatttcagaa cggggaatat
     2281 cttttcagca ggagagcgac cggcccactg tatgtcctga atgtggcata cgcttatttt
     2341 tcgcttttct actgcctgta cttcttttcc ctggtgtgga taaaaagtcg ctttcggctg
     2401 aacagcccct atttcctcct tcttgcggga caacttctct ccgcggcatt gttcgggctg
     2461 tacctggcgg ggatgacgcc gctctgggtc gacatgctgc cggtgggcta tttggttcgt
     2521 gccgtgtttt gcgccgttgc catctttaaa tatgatgttt tggaaataaa agaaatatac
     2581 aataaagaga tcttttcgga aataaaagag gggcttatgc tcgtttcctg tgacgggctg
     2641 ctgatcgact acaacaggtc tgcccggaca gtcttcgaat ggctcaatga aaaaaacagg
     2701 ggggctccca ttcattctct ggattcgcgg ttcaacgtca gtacgggggc caaaaatttc
     2761 gatatcggcg tcggcagagg ggatgaagag cgaatttacg agtttcgcct cacggagttg
     2821 aaggaaggaa gaaaaacagc ggctttcctc tatatttttc tcgatgtcac cgataaacag
     2881 cgcctgatcg aaaaattgag gtacatggcg gaccatgatt cccttacggg gttattcaac
     2941 aggggaagaa tcctggttga agccaatagg cttctttctg cccgcaagct ggaaggccgc
     3001 attttttcga tctgcatgat tgatgccgat catttcaaaa acctgaatga cagctgcggg
     3061 catatcgccg gaaacaggat gctctgtgaa ctcgcggccg tcttcaggcg gattgcggga
     3121 gagcgcggaa tcattgggcg ctacggaggg gaagaattcc tggttctcct tcctgatgcc
     3181 ggcgcaaaag aggccgcgga gacggccgaa aagctgcggg ccgggatcga agcggcgaca
     3241 gtggatttcg atggaaaaac gcttcaggtc acgatcagca tcggtgtcac gtccgcaggt
     3301 ttgcacccct ttgaagagga gacgatacac agccttgtca gccgagccga cagggcgctg
     3361 taccgggcga aaaacagcgg caggaaccgc gtctgcgtct tcggggaaga cgacctgtga
     3421 attgccgccg gttcgccggg gaaaaacgga aggcccgggg cctgctgaca ggccccgggc
     3481 ctttttgcct tttttgcagc cggtttgaat gtgtcgtcct ttgccggagg gcaggggccg
     3541 cggcccttta ccctcggatg cagaccgagt ccaggtccct cgggaaatgg ttcagcgctt
     3601 cgcccgttcc gtcggccctg accgccacga tgttttcgat cctgaccccg aacttgcccg
     3661 gaaggtagat gcccggttcc acgctgaaga cgttccccgg ctccaggggc cggtcgttgc
     3721 cctcgatcat gtagggggat tcatggaccg caaggccgat cccgtggccg acccggttga
     3781 agaagtattc gccgtacccg gcgtcgatga tgacctgccg ggcagccctg tccacgtcct
     3841 gccccgtggc gccgggcttt accgccgctt ctcccgctgc ctgggcctgt ctgacgatct
     3901 cgtacacctt cctctgctcc ccggtggggg tgcccgtgaa gaaggtccgg gtagtgtccg
     3961 agcagtagga tttgtaccgt ccgccgaggt cgaggatgat
//
LOCUS       DAOTHD010000069         3961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_27039_length_3961_cov_3.340758, whole genome shotgun sequence.
ACCESSION   DAOTHD010000069 DAOTHD010000000
VERSION     DAOTHD010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27039_length_3961_cov_3.340758"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..862)
                     /locus_tag="P5219_01970"
     CDS             complement(5..862)
                     /locus_tag="P5219_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25552.1"
                     /translation="MKKKAGKVFSIAAVLLFLLFAPASPGHSWELYVSSPWLSTIARF
                     IGGVNVSVKAIQEWNEEGTSIRKIRSRNIPDSSRIVVLDAFEAKSLGLDGKRFTSIFM
                     LYGTVPFDRARADYHFSDPSVLPFIAQRVLTAMSQYDPGNYSYYQRRLSEFQTRLDST
                     VLVGRQLLKDYPVFDLSGGFTSLLSAAGCTLLPGDEKVKMWSRGEDLQNLQAAVADAI
                     KVRIPVIVDPSTPKPVREALKGNKDVLFLGRPGQEQDLLLFFHDQFLLLWNRLAPLRQ
                     HRQQEGQAA"
     gene            complement(859..1578)
                     /gene="rnc"
                     /locus_tag="P5219_01975"
     CDS             complement(859..1578)
                     /gene="rnc"
                     /locus_tag="P5219_01975"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRX25553.1"
                     /translation="MNYAAWFSGELEKLQDCLGYRFSDVGKLENALVHSSFAYEKGKT
                     VHNERMEFLGDAVLELGVSRFLYESFPSFDEGRLTRSRAAIVCGKSLAEWGREIGLSE
                     LLRTGKGLETEQVRQGSLCSDAVEALLGAVFLDGGYFPAMAVIGRYLAFHLSRHSISG
                     AENDPKSALQMLAHGKGLSSPVYEVLSVSGQPHTPLFSVRVMIGNEEAGSGDGRNRKA
                     AEFAAARNAIFLLNREGSEPK"
     gene            complement(1594..2832)
                     /gene="fabF"
                     /locus_tag="P5219_01980"
     CDS             complement(1594..2832)
                     /gene="fabF"
                     /locus_tag="P5219_01980"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRX25554.1"
                     /translation="MRRVVVTGLGVVSPIATGRENYWQALKEGKNGIAPYTTFDVTDC
                     PVPFGAEIKDFDPSRWMDNKEAKRSDRVIQFAVAAADLAVADAKLDTASLDPHKFGVY
                     IGSGQGGIETTFNNFKTMMEKGAKRVSPFFIPMMISNMSTAYVAIKHQARGPNMCVVT
                     ACATSVHSMGEAYHTIIRDDADVILAGGTEAALRSISIAGFAAMKAISTRMDAPEKAS
                     RPFDADRDGFVMGEGAGVLVLEELEHALKRGAHIYAELVGYGSTCDASHITAPDPEGE
                     GAARAMRKAMAHAKWLPEDVDYINAHGTSTPLNDKMEAMAIRKVFGEHTDKVLVNSTK
                     SMIGHCLGAAGALESVAAMQSIEEGVIHPTINLDTLDPECPINVVGAGAVKKNVSRLL
                     VNSFGFGGHNGVVAFQKFEE"
     gene            complement(2933..3184)
                     /gene="acpP"
                     /locus_tag="P5219_01985"
     CDS             complement(2933..3184)
                     /gene="acpP"
                     /locus_tag="P5219_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRX25555.1"
                     /translation="MKKEEMLARLKEIIIDRLDVEEDQIVPEASFVEDLGADSLDIVE
                     LIMGIEEEFDIEIPDEDAEKLTTVGEAMNYTLGKLGVDE"
     gene            complement(3211..3960)
                     /gene="fabG"
                     /locus_tag="P5219_01990"
     CDS             complement(3211..3960)
                     /gene="fabG"
                     /locus_tag="P5219_01990"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRX25556.1"
                     /translation="MSRFANKVAVVTGGGRGIGQEIARKLASEGAKVAVVSRSASSCG
                     KAADEINAEFPGAAKAYPVDIADHAAVQELAKQVCEDFGSVNILVNNAGVTRDTLLMR
                     MKEDDWDTVLNTNLKGAFNTVKGFMRVLMKAEDARIINIASVIGLIGNAGQANYSASK
                     AGLIGFTKAVAREYAGKGITANALAPGYISTEMTDSLPEAARNTLLQQVPAGRPGTPE
                     DVAAAVAFLASGDASYINGQVIAVDGGMTMC"
BASE COUNT          816 a         1125 c         1071 g          949 t
ORIGIN      
        1 cacctcaagc ggcctgccct tcctgctgcc gatgttgcct gagaggagcc aggcggttcc
       61 ataaaaggag aaactggtca tggaaaaaaa gaagcaaatc ctgttcctgg ccaggacgtc
      121 ccaggaacag gacgtctttg ttccctttca gagcctccct gaccggtttc ggcgtgctgg
      181 gatccactat caccggaatt ctcaccttta ttgcgtcggc aacggcagcc tgaagattct
      241 gcaggtcctc tcccctgctc cacattttga ctttttcgtc tcccgggagc agcgtgcatc
      301 ccgctgctga aagcagactg gtaaaaccac cggaaaggtc gaacacaggg taatctttca
      361 ggagttgtct tccgacgagg acagtgctgt caagcctggt ctggaattcc gacaatcttc
      421 tctggtaata ggaataattc ccgggatcgt actgggacat ggcggtcagc actctttggg
      481 ctatgaaggg gagtaccgat ggatcggaaa agtggtagtc agctcttgcc ctgtcgaaag
      541 gcactgtccc atagagcatg aagatgctgg tgaaccgttt accgtcaagg ccgagtgatt
      601 tggcctcgaa tgcgtccagg accacgattc tggagctgtc cggaatattc cggctgcgga
      661 ttttcctgat ggaagttcct tcttcgttcc attcctggat cgcctttacg gacacgttca
      721 ccccgccgat aaagcgggca atcgttgaaa gccatggcga ggacacgtac agctcccatg
      781 aatgcccggg cgatgcggga gcaaaaagga ggaacagaag aacagcagcg atggaaaaaa
      841 cttttccagc tttttttttc atttcggctc cgagccctcc cggttcagca aaaaaatagc
      901 gtttcgggcg gccgcaaact ccgcggcttt cctgtttctg ccgtcacccg aaccggcttc
      961 ttcatttccg atcatgaccc ggactgaaaa gagaggcgtg tgaggctggc cagagactga
     1021 aaggacctca tacacagggc ttgacaagcc ttttccatgg gcgagcatct ggagggcgga
     1081 cttcgggtcg ttttctgcac cgctgatgga atgccgtgaa agatgaaaag ccaggtaacg
     1141 gccgatcacc gccatggccg ggaagtaccc tccatcgaga aaaacagccc ccaaaagggc
     1201 ctccaccgca tcggaacaaa gggagccctg acgcacctgt tctgtttcaa ggcctttccc
     1261 cgttctgagc agctcgctca gcccgatttc ccttccccat tcggcaaggg attttccgca
     1321 gacaatggca gcccgggaac gggtcagcct gccttcgtca aaagagggaa aagattcata
     1381 cagaaaacgg gagacgccga gctcaagcac ggcatctccc aaaaactcca ttctctcgtt
     1441 atgcaccgtt ttgccctttt cgtatgcgaa ggaagaatgc acaagagcat tctccagttt
     1501 cccgacatcg ctgaaacgat acccaaggca atcctggagt ttttccagtt ctccggaaaa
     1561 ccaggcagcg tagttcatgg agtaagaatg ggtctactcc tcaaatttct ggaaggcaac
     1621 cacgccgttg tggccgccga atccgaaact gttgacaagg agccttgaaa catttttctt
     1681 caccgctccg gcaccaacga cattgatggg gcattcagga tcgagggtat caagattgat
     1741 tgtcggatga atgactccct cctcgattga ctgcatggcg gccacacttt ccagggcgcc
     1801 tgcggcaccg aggcaatgcc cgatcatgga cttggtcgaa ttgacaagca ccttgtcggt
     1861 atgttcgccg aaaactttcc ggattgccat ggcctccatc ttgtcgttga ggggagttga
     1921 tgttccatgg gcgttgatgt aatccacgtc ttcaggcagc cattttgcgt gggccattgc
     1981 ttttcgcatg gctctggcag cgccttcgcc ttcaggatcg ggggccgtga tgtggcttgc
     2041 atcgcaggtg gagccgtagc cgacaagttc ggcgtaaata tgagcacccc ttttcagcgc
     2101 atgctccagt tcttcgagga caagaacacc tgccccttct cccatgacaa acccgtcgcg
     2161 gtctgcatcg aatggacggg aggctttttc aggagcgtcc attctggtcg agatcgcttt
     2221 catggcggcg aagccggcga tgcttatgct cctgagggcg gcttcagtgc ctccggcaag
     2281 aatgacatcg gcatcgtccc gaatgatggt gtggtatgct tcgcccatgc tgtggaccga
     2341 cgtggcgcag gctgttacga cgcacatgtt cggtccccgg gcttgatgct ttatcgccac
     2401 gtatgccgtg gacatgttgc tgatcatcat gggaatgaaa aagggactga ctcgtttcgc
     2461 ccctttttcc atcatggtct tgaagttgtt gaatgtcgtt tcaatgccgc cttgtccgct
     2521 tcctatgtag actccgaatt tatggggatc caggcttgcc gtatcaagct tcgcgtcagc
     2581 aacggccaaa tccgctgctg ccactgcaaa ttgaatgacc cggtcggatc gcttggcttc
     2641 cttgttgtcc atccatcggg acgggtcgaa atccttgatt tccgctccga atggaacggg
     2701 gcaatctgtg acatcaaaag tggtatacgg cgcgatgccg tttttgcctt ccttcaatgc
     2761 ctgccagtaa ttttctcttc ctgttgctat ggggctcacg actcccagcc cggttacgac
     2821 aacccttctc aaaacgcttc actcctccgc agcagggttg gaacatgata ttcagaagga
     2881 ttttggcggg aaatccgtcg ggattcccgc caaaaccgtc tttctcccgg gcttactcgt
     2941 ctacgccgag ttttccgagg gtgtagttca ttgcttctcc caccgtggtc agcttttcag
     3001 cgtcttcgtc gggaatttca atgtcaaatt cttcttcgat gcccataatg agctcgacga
     3061 tgtccagtga atctgcaccg agatcttcca caaaggaggc ttccggcact atctgatcct
     3121 cttcgacatc aagcctgtcg atgatgattt ccttcaaacg ggcgagcatt tcctcttttt
     3181 tcatcttcgt tcaccccctt ccattgtatt tcagcacatg gtcataccgc cgtcaacggc
     3241 gattacctgt ccgttaatat acgacgcatc ccccgaagcc aggaaggcga ctgcagcagc
     3301 cacatcttcc ggcgtccccg ggcggccggc gggaacctgc tgcaaaagtg tgttccgtgc
     3361 ggcttcagga agggaatccg tcatttctgt agaaatatac cccggagcca gggcgtttgc
     3421 cgtgatgccc ttgccggcgt attcccgcgc cacggccttg gtgaaaccga tgagcccggc
     3481 tttcgacgcg gagtagttcg cttgtccggc gttaccgatg aggccgatga ccgaggcgat
     3541 gttgatgatg cgcgcgtcct cggctttcat cagcacgcgc atgaagcctt tgaccgtgtt
     3601 gaaggcaccc ttgaggttgg tgttgagcac ggtgtcccaa tcgtcctcct tcatgcgcat
     3661 gagcagggtg tcgcgggtga cgccggcgtt gttgacgagg atgttcacgc tgccgaagtc
     3721 ctcgcaaacc tgcttggcga gctcctggac cgcggcgtgg tcggcgatgt ccacgggata
     3781 agctttcgcg gctccgggaa actcggcgtt gatctcatcg gccgccttgc cgcagctcga
     3841 cgcgctgcgg ctcacgacgg cgaccttcgc tccctcggag gcgagcttgc gggcgatttc
     3901 ctggccgatg cctcggcctc ctccggtgac gacggcgact ttgttcgcga aacgtgacat
     3961 g
//
LOCUS       DAOTHD010000070         3959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_27054_length_3959_cov_3.671619, whole genome shotgun sequence.
ACCESSION   DAOTHD010000070 DAOTHD010000000
VERSION     DAOTHD010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27054_length_3959_cov_3.671619"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="P5219_01995"
     CDS             <1..382
                     /locus_tag="P5219_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HRX25557.1"
                     /translation="MEVSFLLVPGINDDMEAFGRMVQWLASLDPRPVLHVSRYFPARK
                     WSAPPPSNKLVGEYCTLARKELPWVYPGNTGERTETRCPSCGGLLIARREYSITGNNL
                     DSQGKCRACGMQSQIIITEYSGAD"
     gene            383..1405
                     /gene="mltG"
                     /locus_tag="P5219_02000"
     CDS             383..1405
                     /gene="mltG"
                     /locus_tag="P5219_02000"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRX25558.1"
                     /translation="MKKRLFLLFFALAAAALGFAYRTIYYPAEWWHEYLPAGEGEPIQ
                     VFVRQGMNARRAADEFESAGVLAGGKASDLARWMTRFSIDRRLKPGMYSIRRGSPWEV
                     ARQLEKAEPSVSSATIVPGTDIFSFPGIFAPPLAPEAMEKILARNDLFPKEVASLLPS
                     EQEGRIAFLLPETVQTSENSGEEVVSSAARLWWNRIGSRIPEEKRTAEYLLEMAIISS
                     LIEREALWDEERALIAGVIENRREKKMPLQIDATVVYAWKKEGRNLTRVLYKDLEIDS
                     PYNTYKIAGLPPAPICMPSEESWLAALSPEKTKYLYYVAGQDGRHLFSETLRGHQRNI
                     RKVRGK"
     gene            1402..1767
                     /locus_tag="P5219_02005"
     CDS             1402..1767
                     /locus_tag="P5219_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25559.1"
                     /translation="MTFAGRKDIAPDMGRLLLALPRDSIVVFSWTVSEYDGLGFVTAE
                     NGDEKAGGKESRRRIRGGEVGRDVVSLFFPSEKRQDVLELIDALKAEGISLELLREEE
                     PDFPYGAKTEDNPEEFDSI"
     gene            1764..3125
                     /locus_tag="P5219_02010"
     CDS             1764..3125
                     /locus_tag="P5219_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HRX25560.1"
                     /translation="MSVNARLGKALKSLLAEGADFADLYFETSSSHSFVYEEGAFEEI
                     SSSSSEGTGARVVRGESTSHVHAPGVGTATGFSCLDRAASNSGLLLPPCGGSDVRVVG
                     VEETLAPPDFTFFRDVDGRIRNGSMWVKQVSMHLETACRSFAVFNSEDTFAGDRRQYT
                     LFSVEVVVEKGGVLQTGYESEALSLGSSDFFRKVSPLKVAEEALARALLMLDAPECPA
                     GTMPVLLSGEAGGTMVHEACGHGLEADIIRKDFSVYRGKIGQAAASPLVTLVDDGSLP
                     GLLGSGTCDDEGTPCRRNVLIENGVLKSYLTDRTSARMDGLPLTGNGRRSSFRSIPQP
                     RMTNTYIEPGETSPEDMLRGMKQGLFVRKMGGGEVDPTSGDFVFHVTEGYLVQDGRIL
                     HPVRGAVLTGNGPDVLFKIEAVGNDLHFLPGMCGKSGQSVPVSDGQPSLLLHSMVIGG
                     AGA"
     gene            3127..>3959
                     /locus_tag="P5219_02015"
     CDS             3127..>3959
                     /locus_tag="P5219_02015"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRX25561.1"
                     /translation="MSFFGNKEAARERRSRGKRKRNGGGILSLWIEVFAFLVLAFLLY
                     VLASIFSLRTGAWGEQIRFSLLRNWGGAVIIPVLFGGYLCVSYLLKTGAKGIIRQFLG
                     TALLFFCSALLFGLFRMAAVFQGVAVLSPGYLGDGLAVFFTRNIGSLGTLLLGAAFLV
                     LSASLYGFFQPAAVIRKVSLLVKTISSEKWKRMPFPEDEAPQPSPVPAEEDSVPQVLP
                     DGNHPPRQWSFSSQAEQEEPAAEQEPGGNTIPGSGAAEEAVPLVQEETEIYGRQDHRI
                     FP"
BASE COUNT          850 a         1075 c         1171 g          863 t
ORIGIN      
        1 catggaggtc tccttcctcc tggtacccgg cataaacgac gacatggagg ccttcggccg
       61 catggtccaa tggcttgctt ctctcgaccc ccggcccgtt cttcatgtct cccggtactt
      121 ccccgccagg aaatggtccg cgccgccccc gtcgaataag ttggtggggg agtactgtac
      181 cctggcccgg aaggagcttc cctgggtcta tcccggcaat acgggagagc gaaccgaaac
      241 gagatgtcct tcctgcggag gtcttcttat cgccagaaga gagtattcca taaccgggaa
      301 caacctcgat tcccagggaa aatgcagggc atgcggcatg cagtctcaga tcatcataac
      361 cgaatactct ggagctgatt gaatgaaaaa acgattattc ctcctgttct tcgctctcgc
      421 agctgctgcc ctcgggtttg cctaccggac aatctactat ccggcggaat ggtggcacga
      481 atatctgccc gccggtgagg gagagccgat ccaggtcttt gtgcggcagg gcatgaacgc
      541 caggagggcg gccgatgaat ttgaatccgc cggggttctg gcgggaggaa aggcttccga
      601 cctggcgaga tggatgaccc ggttttccat tgaccggcgg ctgaagcccg gaatgtactc
      661 catacgaagg ggatctccct gggaagtggc caggcagctg gaaaaagcgg aaccctccgt
      721 gtcatccgcc accattgtgc cgggaacgga tattttctct tttcccggta tttttgcacc
      781 tcctcttgct cctgaagcca tggagaagat ccttgcacga aacgacctgt tcccgaagga
      841 ggttgcttcc ctgctgcctt ccgaacagga gggaaggatc gcattcctgt tgccggagac
      901 cgtccagact tcggaaaaca gcggcgagga agtcgtgtcg tcagctgcgc gcctgtggtg
      961 gaacaggata ggatcacgaa tccccgaaga aaagagaacg gcggaatatc tcctggaaat
     1021 ggcgatcatt tcctccctca tagaacggga ggccctctgg gatgaagaac gggcactcat
     1081 cgccggagtc attgaaaaca ggagagaaaa gaaaatgcct ctccagatcg acgccaccgt
     1141 ggtgtatgca tggaagaaag agggaaggaa cctgactcgt gttctctaca aggatctgga
     1201 aattgattct ccctacaaca cctataaaat agcgggactg cctcccgcac ctatctgcat
     1261 gccgtcggag gagtcgtggc tcgccgctct cagcccggaa aagacaaaat atctgtatta
     1321 cgtagccggt caggatggac ggcatctttt ctccgaaacc ctcagaggtc atcagaggaa
     1381 tattcggaag gtgcggggca aatgaccttc gccggcagaa aggatatcgc tcccgacatg
     1441 ggaaggctgc ttctggctct tcccagggat tccatcgtgg ttttcagctg gaccgtttcc
     1501 gaatacgacg gcctcggatt cgtaacggcg gaaaacggag atgagaaggc cggcggaaag
     1561 gaatcccgaa ggcggattag gggcggagaa gtggggcggg acgttgtctc cctcttcttc
     1621 ccttcggaga aacggcagga cgtgttagaa ttgattgatg ccctgaaagc agaagggata
     1681 agcctggagc ttcttcgcga ggaggagccc gactttccct atggggcaaa aacagaagac
     1741 aatccggagg aattcgattc gatatgagtg tcaacgccag gctgggaaaa gccctgaagt
     1801 ccctcctcgc agaaggtgcg gatttcgcgg atctttattt cgaaacatcc tcttcccatt
     1861 ctttcgtgta tgaggaagga gccttcgaag aaatttcctc ttccagttcc gagggaacgg
     1921 gcgccagggt cgtcagggga gaatccactt ctcacgtcca tgcgcccggt gtgggtaccg
     1981 cgacgggctt ttcatgtctc gatagggccg cctcgaatag cggtctcctt cttcctccct
     2041 gcggggggag cgatgtccgg gtggtcggcg tggaagaaac cctggctccg ccggacttta
     2101 cctttttcag ggatgttgac ggaaggatca gaaacggctc tatgtgggtg aagcaggttt
     2161 ccatgcatct tgagacagcc tgcaggagtt tcgcagtgtt caacagcgag gatacctttg
     2221 ctggggacag gaggcagtac acccttttca gcgtggaagt ggtggtggag aagggaggag
     2281 tgctgcagac aggctacgag tccgaggccc tttcccttgg cagttccgat tttttccgga
     2341 aggtttcccc cctgaaagtc gctgaagagg cgctggctcg ggctcttctc atgcttgacg
     2401 caccggaatg tcctgcggga accatgcctg tcctgctttc cggcgaggcc ggagggacca
     2461 tggtgcatga agcctgtggc catgggcttg aagctgatat catccggaag gacttttctg
     2521 tctacagggg gaagatcggg caggcggcgg cgagtcccct ggtgaccctc gtcgacgacg
     2581 gttcgcttcc cggacttctt ggaagcggaa cctgcgacga cgagggaaca ccctgcaggc
     2641 ggaacgttct catcgaaaac ggtgtgctga aaagctacct tactgacagg acgtcggcac
     2701 ggatggacgg ccttcccctt acgggcaacg gcaggaggag ttccttccgt tccattcctc
     2761 agccccgaat gacgaacacc tatattgagc ccggcgaaac ctctcccgag gatatgctca
     2821 ggggcatgaa acaggggctc ttcgtccgga agatgggagg aggagaagtg gaccccacct
     2881 ccggcgattt tgtttttcat gtcacagaag gatacctggt acaggacggg cgaatccttc
     2941 atcccgttcg tggagccgtc ctcactggga atggtcccga cgttctgttc aaaatcgaag
     3001 cggtggggaa cgatctccac tttttgccgg ggatgtgcgg gaaatcggga cagagcgttc
     3061 ctgtttcaga cggacagccg tccctgctgc tccacagcat ggtgatagga ggcgcgggag
     3121 cctgacatga gttttttcgg aaataaagaa gcagcccgcg agcgcagatc aagaggaaaa
     3181 agaaaaagaa acggcggggg catcctgtcc ctctggatag aagtctttgc ttttctcgtc
     3241 ctcgcttttc tgctctatgt gctcgcatcg attttctcgc tccggacggg agcctgggga
     3301 gagcagatac ggttttccct gctgagaaac tggggaggag cagtcatcat tcccgttctt
     3361 ttcggaggat acctctgcgt ctcctatctc ctgaagacgg gagcaaaggg cattatccgg
     3421 cagtttctgg gcacggccct cctgtttttc tgctcagccc tcctcttcgg gctcttccgg
     3481 atggccgcgg tattccaggg agtggccgtc ctgtccccgg gatacctggg tgacggcctc
     3541 gccgtttttt tcacccgcaa catagggtct cttggaaccc tgcttctcgg cgcagctttt
     3601 ctggttcttt cagcaagcct ttacggtttt ttccagcctg cggcggtgat acggaaggta
     3661 tcccttctgg tgaagaccat ttcgagcgaa aaatggaagc gtatgccctt ccccgaggac
     3721 gaggccccgc agccctctcc cgttcctgct gaggaagact ctgtccccca agtgcttccc
     3781 gatgggaacc atcctccccg gcaatggtca ttctcctctc aggcagagca ggaagagcca
     3841 gcggcagaac aggaacccgg gggaaatacc atccccggat ccggcgcggc agaggaagcc
     3901 gtacctttgg ttcaggaaga gactgaaatc tacggccgtc aagatcatag gatattccc
//
LOCUS       DAOTHD010000071         3927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_27347_length_3927_cov_2.859762, whole genome shotgun sequence.
ACCESSION   DAOTHD010000071 DAOTHD010000000
VERSION     DAOTHD010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27347_length_3927_cov_2.859762"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /locus_tag="P5219_02020"
     CDS             complement(<1..1332)
                     /locus_tag="P5219_02020"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRX25562.1"
                     /translation="MNSGRIITVNGPVVRATGMRDFAMREMVTVGKLSLLGEIIRLDG
                     DDALIQVYEDTAGVTVGEPVAGSGSPLSVSLGPGLMGSIFDGIGRPLARLLEKEGAFV
                     TRGVSVPQVDPDRTWEITPLGKVGDVVTPGTVLASVKETSLVEHRILVPAGLEGEILS
                     ILPAGFHRASSVVAKIRDSRGKERSVPMISRWPVRTPRPIAERLLPDEPLLTGQRVID
                     GLFPLSKGGVAAIPGGFGTGKTVTQHQLAKWSDARIVIYIGCGERGNEMTQVLEEFPV
                     LEDPRSGRPLMERTILIANTSNMPVAAREASIYTGITLAEYYRDMGYDVALMADSTSR
                     WAEALREISGRLGEIPAEEGFPAYLPTRIAEFYERAGKVTTLGGTRGSISIIGAVSPP
                     GGDFTEPVTRHTKRFIRCFWALDRNLANARHFPAIGWLESYSEYAREVEDWF"
     gene            complement(1343..1990)
                     /locus_tag="P5219_02025"
     CDS             complement(1343..1990)
                     /locus_tag="P5219_02025"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HRX25563.1"
                     /translation="MTDSIAGDGRNYEVRNPEKLASLKGLLLKKADDEREALLESARR
                     ESSAWLEEQNAALDRMVEQIHTEAVKRAEEISKRQISGAEMARTKERLRLQNSLLDEA
                     VTMLQKELTALRIHPSYPGVLAGLAFEAAEKLPAGTEVRIQLASEDEALGETLAARLR
                     EKRPDLSVSFDTDGAPILGGVWLSAPDGSWRSPADWREIIAEVKDSLAERILSIL"
     gene            complement(1987..2301)
                     /locus_tag="P5219_02030"
     CDS             complement(1987..2301)
                     /locus_tag="P5219_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HRX25564.1"
                     /translation="MKGFLISDNHDSLVLLRLAGIPGVEVHGPEETAAALEDVLANRP
                     DVGVLVITEKAAAQVPAMVKTLRERGETPLLVEIPDRHGSMRQGDFLTRYIRDAIGVK
                     IE"
     gene            complement(2321..2734)
                     /locus_tag="P5219_02035"
     CDS             complement(2321..2734)
                     /locus_tag="P5219_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HRX25565.1"
                     /translation="MSGLALIAGGAVLACGAGFYLQGRTVRSPRAVLGSVLAVLAVLL
                     GAGLVLTLLPVPSAAAETTAASAASGLGFLGASLATGLACLGAGLAVAVVGAAALGVV
                     GEKPSMLGTTLIYLGLAEGIAIYGVIVSLLILGKL"
     gene            complement(2754..>3927)
                     /locus_tag="P5219_02040"
     CDS             complement(2754..>3927)
                     /locus_tag="P5219_02040"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HRX25566.1"
                     /translation="LERLYCAVHSLQRVYALCQKRGEMSGIFVLSGVIPAPTLGEVAE
                     KVEQLGPNTLLLSEKGAELEKRGRQLPTLLRNNFLVRTFQEIVALYSLPSYGETDPSP
                     LVALSFCLFFGFMFGDVGHGFLLAAGAWWLARKGILKKAFASVIRIAGVSAMVFGFLY
                     GSVFGSEEILPSLWTSPMHGISDLIQTSLFVGVAFLSMGIIINILALKREGRTGKMLF
                     DGEGVAGLLFYWTAAVTAAMAFTSGGGAPKPLLAVLGLLFLVILFSSVLERLLFGPQE
                     KDEGGVVHTFTVFHDLLSFLSNTASFVRLAAFALNHAGLSAAVFMLSDMVHSLPGGAL
                     FRAAVLLLGNIVIVGLEGLIVFIQTLRLEYYEFFSKFYHGGGRPFAPVTWNAKRQD"
BASE COUNT          695 a         1261 c         1315 g          656 t
ORIGIN      
        1 gaaccagtct tccacttccc gggcatattc gctgtaggat tccagccagc cgatggcggg
       61 gaaatggcgg gcgttggcca ggttccggtc cagggcccag aagcagcgga tgaaccgctt
      121 ggtgtgccgg gtcaccggct cggtgaagtc cccacccgga ggggagacgg cgccgatgat
      181 ggagatgctg ccacgggttc ctcccagggt ggtgaccttt cccgcccgtt cgtagaactc
      241 ggcgatgcgg gtggggaggt aggcgggaaa tccctcctcg gcgggaattt ccccgaggcg
      301 gccggaaatc tccctgaggg cctcggccca ccgggaggtg gaatctgcca tgagggccac
      361 gtcgtagccc atgtcccggt agtactccgc cagggtgatg cccgtgtaga tggaggcctc
      421 cctggccgcc acgggcatgt tggacgtgtt ggcgatgagg atggtgcgct ccatgagagg
      481 cctgcccgag cgggggtcct ccaggacggg aaattcctcg agcacctggg tcatctcgtt
      541 gccccgctcg ccgcacccga tatagatcac gatccgggcg tcgctccact tggcgagctg
      601 gtgctgggtc actgtctttc cggtgccgaa gcctccggga atggccgcca cgccgccctt
      661 ggagaggggg aagagaccgt cgatgacccg ctggcctgtg agcagaggtt cgtcgggaag
      721 aagccgctcg gctatgggcc ggggtgtccg cacgggccac cgggagatca tggggacgga
      781 ccgttccttt ccccgggaat cccggatctt tgccaccacc gacgacgccc tgtggaaccc
      841 cgccggaagg atggagagaa tttccccttc cagcccggcg ggcacgagaa tccggtgctc
      901 cacaagagag gtctccttca ctgacgcaag caccgttccc ggcgtgacca cgtcgcccac
      961 cttgcccagg ggggtgatct cccatgttct gtcaggatcg acctggggga cggagacgcc
     1021 ccgggtgaca aaggcccctt ccttctccag aaggcgggcc aggggccggc cgatgccgtc
     1081 gaagatggag cccatgaggc ccgggccgag ggacacggaa agggggctgc ccgatcccgc
     1141 caccggttcg ccgacggtga cgcccgccgt gtcctcgtac acctggatga gggcatcgtc
     1201 cccgtcgagg cggatgatct cgcccagcag ggagagtttc cccacggtga ccatttcccg
     1261 catggcgaag tcccgcatgc ccgtcgcacg gaccacgggt ccgttcaccg tgatgatccg
     1321 tccggagttc atggccgcct cctcacagga tggagaggat ccgctcggcg agggaatcct
     1381 tcacttcagc gatgatttcc cgccagtcgg cgggggagcg ccacgagccg tcgggagcgg
     1441 agagccagac tccacccagg atgggggcgc cgtcggtgtc gaaggaaacg gaaagatccg
     1501 gccgcttctc ccggaggcgc gccgccagcg tctcccccag ggcttcatcc tcggaagcaa
     1561 gctggattct gacctccgtc ccggcgggga gcttttcagc cgcctcgaag gccaacccag
     1621 cgaggacccc aggataggac ggatgaatcc ggagggcggt aagctctttc tggagcatgg
     1681 tcaccgcctc gtccaggagg gaattctgga ggcgcaggcg ctccttggtc cgggccatct
     1741 cggcgccgga aatctgccgc ttggatatct cttcagcccg cttcaccgcc tcggtgtgga
     1801 tctgctccac catgcggtcc agggcggcgt tctgctcttc gagccatgcg gaggactccc
     1861 gcctggcgga ctccaggaga gcttcccgtt cgtcgtcggc cttcttgaga agcagccctt
     1921 taagggaggc cagcttttcg gggttgcgga cctcgtaatt ccggccgtct ccggctatgg
     1981 aatcggtcat tctatcttca ctccaatcgc gtcgcggatg taccgcgtca gaaaatcgcc
     2041 ctgccgcatg ctgccgtggc ggtcgggtat ctccaccagg agaggggttt ctcctctctc
     2101 ccggagggtc ttcaccatgg cgggaacctg ggcggcagct ttttcggtga tgaccaggac
     2161 ccccacgtcc ggcctgttcg ccagcacgtc ctccagggcg gcggcggttt cctccgggcc
     2221 gtggacctcc acgccgggga tgcccgccag gcggagcagc accagggagt cgtggttgtc
     2281 gctgatgagg aatcccttca tgttcccggg agcctccggg ctacagcttg cccaggatca
     2341 gcagggagac gatgactcca tagatggcga tgccttccgc caggccgagg tagatgaggg
     2401 tcgtccccag catggagggc ttctccccca ccacgcccag ggcggctgcg cccaccaccg
     2461 ccacggcgag gcccgcaccc aggcaggcca gcccggtggc gaggctcgcg ccgaggaacc
     2521 ccagccccga cgcggcggag gcagccgtcg tctcagcggc cgccgagggg acgggcagca
     2581 gggtcagcac gaggccggcg ccaagaagga cggccaggac ggcgaggacg ctcccgagaa
     2641 ccgcccgggg ggagcgcacg gttctcccct gaagataaaa tccggcgccg caggcgagaa
     2701 ccgctcctcc agcgatcaat gcaagtccgc tcatgaatgt tttcctcctc agattaatct
     2761 tgtcttttag cgttccaggt tacgggggcg aagggacggc ctccgccgtg gtagaacttg
     2821 ctgaagaact cgtagtactc gagcctcagg gtctggatga agacgatgag cccctcgagg
     2881 ccgacgatga caatgttgcc cagcagcagg accgccgccc ggaagagggc tcccccgggg
     2941 aggctgtgga ccatgtcgct gagcatgaac accgccgcag acagtcccgc gtggttcagg
     3001 gcgaaggccg ccagccgcac gaaggaggcg gtgttgctca ggaaggaaag caggtcgtgg
     3061 aacactgtga aggtgtggac cacgccgccc tcgtcttttt cctgggggcc gaagaggagc
     3121 cgctccagca ccgagctgaa gaggatcacc aggaagagca gcccgaggac cgccaggagc
     3181 ggtttcgggg cccctccgcc cgacgtgaag gccatggcgg ctgttaccgc ggcggtccag
     3241 tagaacagca gaccggccac tccctcgccg tcgaagagca tcttgcccgt ccgtccttcc
     3301 cgcttcaggg ccaggatgtt gatgatgatc cccatgctca ggaaggccac ccctacgaag
     3361 agggaggtct gaatcaggtc ggagatgccg tgcatggggg aggtccacag ggagggcagg
     3421 atttcctccg agccgaagac gctcccgtac aggaagccga agaccatggc ggagactccg
     3481 gcgatgcgga tcaccgacgc gaaggctttt ttgagtatcc ccttccgggc cagccaccat
     3541 gctccggcgg ccagaaggaa gccgtgcccc acgtcgccga acatgaagcc gaagaacagg
     3601 cagaagctca gcgccaccag cggtgaaggg tccgtctccc cgtaggaggg gaggctgtag
     3661 agagccacga tctcctggaa ggtgcgcacg agaaagttgt tcctcagcag agtggggagc
     3721 tgcctgcccc gcttttccag ctccgctccc ttttcactga gcagcagggt gttggggccg
     3781 agctgttcta ccttttccgc cacttccccc agtgtcgggg cggggatgac tccagacaga
     3841 acgaagatgc cgctcatctc accccgcttc tggcagaggg cgtagactct ctggagggag
     3901 tggacggcgc agtagagccg ctccagt
//
LOCUS       DAOTHD010000072         3901 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_27573_length_3901_cov_3.293812, whole genome shotgun sequence.
ACCESSION   DAOTHD010000072 DAOTHD010000000
VERSION     DAOTHD010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27573_length_3901_cov_3.293812"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="P5219_02045"
     CDS             complement(<1..217)
                     /locus_tag="P5219_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRX25567.1"
                     /translation="MNVSIVTAFPGFFRDFLSESMIGRAVRNGLIRIDVVDLRDFGSG
                     NYRQVDDYSFGGGGGMVLLPEVLEKALH"
     gene            complement(214..756)
                     /gene="rimM"
                     /locus_tag="P5219_02050"
     CDS             complement(214..756)
                     /gene="rimM"
                     /locus_tag="P5219_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRX25568.1"
                     /translation="MWTSLRANRVLIGRIVGAQGLRGELKIHALTDNPARFADMKNLR
                     LYGSDGMLRAELTLLSVRFLDSKGIVVAGTKEVTDRNGAESLVGTTVEIFPGERYPLE
                     EGAFWVDDLIGMTAVDHSTGDVLGTVSDVMPAGENDLYAVRDEKGVDHFIPAVKEFIA
                     GVDLEKREMRISLIEGLWDS"
     gene            complement(731..979)
                     /locus_tag="P5219_02055"
     CDS             complement(731..979)
                     /locus_tag="P5219_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HRX25569.1"
                     /translation="MPDYRELVEFIVRRLVTDPDSVRVEAETDDRDVTAVTIHVAQDD
                     VGRVIGKRGATINAVRLLAKAAAVKVNDRVDVDIVEGE"
     gene            complement(982..1257)
                     /gene="rpsP"
                     /locus_tag="P5219_02060"
     CDS             complement(982..1257)
                     /gene="rpsP"
                     /locus_tag="P5219_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRX25570.1"
                     /translation="MAVRIRLARHGRKKAPFYRLVVADSRSPRDGRFIELLGTYDPMT
                     EPAKITVNEERTVYWLTVGATASDTARGLLKKSGVWEKFEAGKKQAE"
     gene            complement(1339..2694)
                     /gene="ffh"
                     /locus_tag="P5219_02065"
     CDS             complement(1339..2694)
                     /gene="ffh"
                     /locus_tag="P5219_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRX25571.1"
                     /translation="MFDALRERLEGVLSKLKSRGRLTESDVDSALREVRRGLLEADVD
                     YKVVKTLVEAIKIRAVGKDVLESITPGQQVAAIVYEELSALMGTEPAPLAISSMPPSV
                     YLMVGLQGGGKTTTTVKIAKKLSAGHKPLVVACDLRRPAAVEQLRILAEQSKIGFFGP
                     AAGESDVLSVVRGAVKFASERLYDVILLDTAGRLHADEDLMEELAAMKRQLPPTEILL
                     VVDAMTGQEAVKVASAFHEKLTITGVVLSKLDGDARGGAALAIRQVTGVPVKLAGVGE
                     GTDALEVFDSRRMAQRIMGMGDVMGLAEKIREMADSGGAEKIAESLKQKKFTLEDLLL
                     QFEQIEKMGPLDKVLEMIPGLSRMKGMGADELDAKRLKQSKAVIQSMTREERREPSII
                     KGSRRRRIALGSGTSVQMVNQLLAQYEQMRKLWKQFGKQGGKGLRLPKGLFGGGGFRF
                     R"
     gene            complement(2675..3031)
                     /locus_tag="P5219_02070"
     CDS             complement(2675..3031)
                     /locus_tag="P5219_02070"
                     /inference="COORDINATES: protein motif:HMM:NF016205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRX25572.1"
                     /translation="MAKEELILSRRLFVNQLYDLYCPLLTEKQREAWELHEFSDLSLS
                     ETAEKLGASRQAVHDLISRSREKLEELETLLGFHRREEGLEEEIRSLRAALEERRGRT
                     GTNSRPEEDHDVRRTS"
     gene            3194..3850
                     /locus_tag="P5219_02075"
     CDS             3194..3850
                     /locus_tag="P5219_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HRX25573.1"
                     /translation="MNGSSFDTARKKHWSVLGPTVAANLSAKGFQAVYAESKKEALDE
                     VLKLIPAGSSVGIPGSVTIREIGAMDALAERGCDVIHHWDPSLTGEERLQRLQDELLA
                     DYFLTSSNAVTKDGMLVNIDGNGNRVSGMAWGKNTLIFVIGLNKVTADIDEAISRTRN
                     TATPPNALRLGLETPCTKTGCCVNCSSEERVCKALLILERATGGRKTHVILVGEDLGF
                     "
BASE COUNT          821 a         1193 c         1013 g          874 t
ORIGIN      
        1 aatgaagggc cttttcaagg acttccggca gaagaaccat tccaccgccg ccgccaaagg
       61 agtagtcatc cacctgtctg tagtttccgg aaccgaaatc ccgcaggtcc accacgtcta
      121 tgcggataag cccgttccgg acagccctcc cgatcatgct ttcggagaga aaatcccgga
      181 aaaaaccggg aaaggcggta acgatgctta cgttcacgag tcccagagcc cctctatgag
      241 ggaaatcctc atttctcttt tttcaaggtc cactccggcg atgaactcct ttaccgccgg
      301 gatgaaatga tcaacaccct tttcgtcccg cacggcgtac aggtcgtttt ctcccgccgg
      361 catcacgtcg gaaaccgtcc ccagcacatc gccggtggaa tggtccaccg ccgtcatccc
      421 tatcaggtcg tcgacccaga aggcaccttc ctcgagagga tagcgctctc cgggaaagat
      481 ctcaacggta gtcccgacga gggactccgc cccgttcctg tccgtgactt ctttcgttcc
      541 ggccactacg atccccttgc tgtcaagaaa acggaccgaa agaagagtca gttcagccct
      601 gagcattcca tcgctcccgt acagcctgag gtttttcata tctgcaaatc gtgcggggtt
      661 atcggtcagg gcgtggattt taagctctcc gcgcagcccc tgcgctccga cgatccgtcc
      721 gatcagaacc ctattcgccc tcaacgatgt ccacatccac ccggtcgttg accttcaccg
      781 cggcagcttt cgcgagcagc cggacagcgt tgatcgtcgc accgcgtttc ccgatcacac
      841 ggcccacgtc atcctgggcg acgtgtatcg taactgccgt cacatcccgg tcgtcggtct
      901 cggcttcgac ccggacactg tccggatcgg tcacgagacg cctgacgatg aattcgacga
      961 gctccctgta gtcgggcatg actattcggc ctgcttcttg cccgcctcga atttctccca
     1021 gacgcccgat tttttgagca gtcccctggc cgtatcggac gcggtggctc cgacggtcag
     1081 ccaatacacc gttctttctt cattgacggt aatttttgcc ggttccgtca tgggatcata
     1141 ggttccgaga agttcgatga accttccgtc cctgggagag cgggaatctg caaccaccaa
     1201 acggtaaaac ggggcctttt tccgtccatg acgggcaagg cgaattcgta ctgccatata
     1261 ccatacacct cctaaaagaa taccggatca cacctgggag acaattttat ctcccggcgg
     1321 tccgtgaccg gacgttgcct acctgaaacg gaaacctccg cctccgaaga gtcccttcgg
     1381 gaggcgaagc ccctttcctc cctgcttgcc gaactgcttc cagagcttcc gcatctgttc
     1441 gtactgggcg agaagctggt tcaccatctg taccgaggtt ccggatccca gggcaatcct
     1501 gcggcgcctg ctccccttga tgatggaagg ttcacgcctt tcttcccggg tcatggactg
     1561 gatgacggcc ttcgactgtt tcagcctttt ggcgtccagt tcgtcggctc ccattccctt
     1621 cattctgctt aagccgggta tcatctccag aaccttgtcc agtggaccca tcttttctat
     1681 ctgctcgaac tggagaagaa ggtcctccag ggtgaacttc ttttgcttga gactttcggc
     1741 gatcttttcc gcaccgccgg agtcggccat ttccctgatc ttttcggcga gacccatcac
     1801 atcgcccata cccatgattc tctgggccat gcggcgggag tcgaacacct caagtgcatc
     1861 ggttccctca ccaacaccgg cgagcttgac cggaacgccg gtaacctgcc ggatggcaag
     1921 ggcagcgccg ccgcgggcat cgccatcgag cttggaaagg accacgccgg tgatggtgag
     1981 cttctcgtgg aacgccgatg ctaccttcac cgcttcctgt cccgtcatgg cgtcaaccac
     2041 gagcaggatt tcagtgggag gaagctgccg cttcattgcc gcaagctcct ccatgagatc
     2101 ttcgtcggca tgaagccgtc cggccgtatc aagcaggatt acgtcataca gcctttccga
     2161 ggcgaacttc acggctcccc tgacgacgga aagaacatcg gactcacccg ccgcaggacc
     2221 gaaaaaccct attttcgact gctcggcaag aatcctgagc tgttccacgg cggccggacg
     2281 cctgaggtcg caggccacca cgaggggttt gtgtcctgcg gaaagcttct tcgctatttt
     2341 taccgtggtg gtggttttcc cgccgccctg gagcccgacc atgaggtaca ccgatggcgg
     2401 catggacgaa atagccagtg gagcgggttc cgtacccatg agagccgaaa gctcctcata
     2461 aactatggct gcaacctgct ggccgggagt tatggattcg aggacatcct tgcccacagc
     2521 ccggattttt atggcttcga caagggtttt aacgaccttg taatccacgt cggcttcgag
     2581 gagtcccctc ctgacctccc ggagggcgct gtcaacgtcg gattcggtta atctgccccg
     2641 gctcttcagt ttggataaaa caccttccag gcgttcacga agtgcgtcga acatcgtggt
     2701 cttcctccgg tcgtgaattc gttcctgttc ttccccgtcg ttcctcgaga gctgcgcgga
     2761 gcgaacggat ctcttcctca agcccttctt ccctgcggtg aaaaccgagg agcgtttcaa
     2821 gttcctccag tttttcccgg cttctggaaa tgaggtcatg aacggcctgt ctgctggccc
     2881 ccagtttttc cgcagtttca gaaagggaaa ggtcggagaa ctcgtgaagc tcccaggctt
     2941 ctcgctgctt ttccgtcagc agggggcagt acagatcgta cagctggttg acaaaaagcc
     3001 ttctcgacag aatcagttct tcttttgcca ccatcgcacc tcccctgaaa cagaggaatt
     3061 atagacggct cttcttcacc tgtcaaggtt tttttcttta cacctctacg gaagcgaaag
     3121 ggggatggac aaagggaatg tttcttgaca tcatagtcgg aatacgacac gaggtactga
     3181 aggaggaaga ttcatgaacg gaagttcttt cgacactgcc cggaaaaagc actggagcgt
     3241 tctgggcccg accgtggccg caaatctttc cgcaaaggga tttcaagcgg tttacgctga
     3301 atcgaaaaaa gaagcactgg atgaagtatt gaagctcata cccgcaggct cctccgtcgg
     3361 aattcccggg agcgttacca tcagggagat tggcgccatg gatgcccttg ccgaaagggg
     3421 atgcgatgtc attcatcact gggatccctc ccttaccgga gaggaacggc tccagagact
     3481 ccaggacgaa ctcctcgcgg attacttcct gacgagctcc aacgccgtga ccaaagacgg
     3541 gatgctcgta aacatcgacg ggaacggcaa cagggtcagc ggcatggcct gggggaaaaa
     3601 cacccttatc ttcgtcatag ggctgaacaa agtaaccgca gacattgacg aggccatttc
     3661 aaggaccagg aacaccgcga ctccccccaa cgccctccgg ctcggacttg aaaccccctg
     3721 cacaaaaact ggctgctgcg tcaactgctc ctcagaggaa agggtatgca aggcccttct
     3781 cattcttgaa cgggctaccg gcggcagaaa aacacacgtc atcctcgtgg gggaagatct
     3841 cggcttctag gggaggaaaa tccgcagctt ccgccgaatg tgctttttgc tgccgtttag
     3901 t
//
LOCUS       DAOTHD010000073         3884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_27717_length_3884_cov_3.160878, whole genome shotgun sequence.
ACCESSION   DAOTHD010000073 DAOTHD010000000
VERSION     DAOTHD010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27717_length_3884_cov_3.160878"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1631)
                     /locus_tag="P5219_02080"
     CDS             complement(<1..1631)
                     /locus_tag="P5219_02080"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRX25574.1"
                     /translation="MDRYEGSNLYRLLTDLVKFRSVSPSREGENAIARFIFDTLSVQP
                     YFRGHPEDVRLLPLDNDPLGRHFVFAVVKAPEPTAETVLLAGHMDVVGVQACGSLAPF
                     AFDPEEYTERIRAADISPEVRKDLETGEWLFGRGVADMKSGLAGGMELLMRAAGDSTG
                     LGANIAFLAVPDEENNSCGMLSAASFLARIQKEEGLRFLACVMLEPTFAAGGEATPSM
                     YLGSIGKINPFFFCAGKETHVGEYYEGFCAAPILSHINLMLDGNPEYADSLFGRSYPP
                     FGCMRQTDLRREYSATIMTRGFAFYSYLTATRLPVQILSDMRSIAAEALDQSISRYRK
                     NAEAFSSLDGTKNPPGNWNPRVIDFADLSGMAADLLGSNFDLFVEESLVSATKDADER
                     DRAITLVETMVELCGLAGPLVVVGFLPPWYPHRSSLGDSEGERIAAWAARETVREAEI
                     RFGETLQLRPFFEGVSDLSYCGFQGPPSEMDVFARNMPGWGKLYSLPTDALAELDIPV
                     LNLGPLGRDAHKNTERIHLRYTLEVFPHLLAFLVK"
     gene            complement(1655..2866)
                     /locus_tag="P5219_02085"
     CDS             complement(1655..2866)
                     /locus_tag="P5219_02085"
                     /inference="COORDINATES: protein motif:HMM:NF014381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HRX25575.1"
                     /translation="MAGMEYLGNKPVAVLGGGATARGHAACTALAGREVRLYELPVFF
                     DGLGCIAKNREIRVSGKQESLYGFKREGLAKIDVVTDDMRKAVEGAGIVIISFPAVGY
                     RAFLEKLIPCLEDGMVIHFTTANFGSLLLRKMMREAGCSKKVIVGEWSSQPYGIRIKM
                     AGGEQMPEVSVNYWAISLRGAALPMTDQDAFFASKKFVPSLDSVVHPVRGDTIADIGF
                     SNVNPILHCPGTILGVGAMENWGVVYGENKYDFSIYSHAYCPSISRVQLALYREECAI
                     AEAMGVGIQDFQEKAFFSRSNILGAEHMGEKFEVPFNEQYTLALGTGPFTIHNRYITE
                     DIPVGCHIFSELAKKFGVDVPVIESMITLASVMTGTDFRKTGVTLEDLGISHMDRQML
                     NAYLREGVYSE"
     gene            complement(2878..>3884)
                     /locus_tag="P5219_02090"
     CDS             complement(2878..>3884)
                     /locus_tag="P5219_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRX25576.1"
                     /translation="AVFGSISGSCAATLSCIGSIMFPRLRENGYPMGHSAALVSCAAP
                     LGLLIPPSSQMILFAWVGQQSVLACFLATVGPGIVLMILLSILNLVMLRKDPNVNVAP
                     QITFQEKIELLGSRGKKAVPALFMPVIVLGGIYGGIMTPTEAAAVAVLYSIPVGFLIY
                     RGLNRNNFLEVIIETATTTGVVMLMMYCIMMLSRIYVQERLPAMIIDFLTAISSNKYV
                     IMLMINIFMIIIGMIMDDVSAMLLCTPILLPVVVSIGIHPIHFAAIIGTNLGLGNVTP
                     PTAPTLYFGGRMAKTPVSQMMKPAMIFILFAWLPTLLAVTYIPELALWLPRLVLGGRL
                     "
BASE COUNT          858 a         1149 c         1073 g          804 t
ORIGIN      
        1 tttttcacga gaaacgccag aagatgggga aacacttcca gtgtgtacct gaggtggatc
       61 ctttccgtgt tcttgtgggc atccctgccg agggggccga ggttcagcac cgggatgtcg
      121 agctcggcaa gggcatcggt gggaaggctg tacagctttc cccagcccgg catgttgcgc
      181 gcgaaaacgt ccatttccga tggcggcccc tggaagccgc agtagctcag gtcggaaacg
      241 ccttcaaaaa aaggacggag ctgcagtgtt tctccgaacc ggatctccgc ctcccgcacc
      301 gtctctcttg ccgcccacgc tgcgatgcgc tctccctctg aatctccgag gctgctcctg
      361 tgggggtacc acggaggcag gaaaccgacg accacaaggg gaccggcgag accgcacagt
      421 tcaaccatgg tctcgacaag ggtgattgcc cggtccctct catcggcatc tttagtcgcc
      481 gagacaagag attcctcaac aaaaagatcg aaattgctcc caagcaaatc cgccgccatg
      541 cctgagagat cggcgaaatc aatcacccgg ggattccagt tccctggagg attttttgtt
      601 ccgtccaggg aagagaacgc ttcggcgttc ttccggtacc ggctgatgga ctggtcaagg
      661 gcctcggcag ctatggaacg catgtccgaa agtatttgca ctggcagcct ggttgccgtg
      721 agatagctgt agaacgcgaa accccgtgtc attatggtcg cggaatactc ccggcggaga
      781 tccgtctgtc gcatacagcc gaaaggcgga taggacctgc cgaaaaggga gtccgcatac
      841 tcagggttgc cgtcgagcat gaggttgatg tgtgacagaa tgggggcggc acaaaatcct
      901 tcgtagtatt cccctacatg agtttccttg ccggcacaga agaaaaaagg attgatttta
      961 cctatgcttc caaggtacat cgagggggtt gcctccccgc cggcggcgaa tgtgggttcg
     1021 agcatgacac atgccaggaa ccgcagccct tcctctttct ggatgcgtgc caggaatgac
     1081 gcagcggaca gcatgccgca cgaattgttt tcctcgtcgg gaacggcgag aaaggctatg
     1141 ttcgctccaa gccccgtgct gtccccggca gcccgcatca gcagttccat gccgccggca
     1201 agcccgctct tcatatcggc aacgccccgt ccgaaaagcc actcccccgt ttcgagatct
     1261 ttccgcacct ccggggaaat atccgcagcc cgaatcctct ctgtatattc ctccggatcg
     1321 aatgcaaatg gagcgaggga accgcatgcc tgaaccccca ccacgtccat gtgcccggcc
     1381 agcagcaccg tttcggcggt gggttccggc gccttcacca cggcgaagac gaaatgtctg
     1441 cccagagggt cgttatccag cggcagaagg cggacgtctt ccgggtgccc gcggaaatag
     1501 ggctgcactg aaagagtatc gaagataaac cgtgcgattg cgttctcgcc ctcccgtgaa
     1561 ggggaaacgc tcctgaattt tacgagatcg gtcagaagcc tgtacaggtt gctgccttca
     1621 tatctgtcca ttttctttgc ctttcccggg aacgtcactc cgaatagaca ccttcccgca
     1681 gataggcatt cagcatctgc ctgtccatat gggaaattcc aagatcttcc agggtaaccc
     1741 ccgttttccg gaaatcagtt cccgtcataa cggaggccag cgttatcata gattcaatca
     1801 ccggcacatc cactccgaac ttttttgcca gttcgctgaa aatatggcat cccacgggaa
     1861 tatcctccgt aatatacctg ttgtggatcg taaaaggtcc cgtacccagc gcaagagtgt
     1921 actgctcatt gaaggggacc tcgaacttct cccccatgtg ctcggccccc agtatattcg
     1981 accgggagaa aaaggctttc tcctggaaat cctggatccc aacacccatg gcttcggcaa
     2041 tggcgcattc ctcccggtac agggcaagct gcacccgtga aatggacgga cagtaggcat
     2101 gggagtaaat ggagaaatcg tatttgttct caccgtagac cacgccccag ttctccatgg
     2161 cgcccactcc caggatggtt cccgggcagt gcagtatggg gttcacgttg ctgaacccta
     2221 tgtccgctat cgtatcgccc cggacagggt ggaccactga atccagggaa ggcacgaact
     2281 tctttgacgc gaaaaaggca tcctggtccg tcatcggcag cgcggcgccc cggaggctta
     2341 tggcccagta gttgacgctt acctccggca tctgttcccc tccggccatt tttatccgga
     2401 tgccgtaggg ctggctgctc cattccccca cgatgacttt cttgctgcat ccagcctccc
     2461 gcatcatttt gcgcaaaagg agagaaccga aatttgccgt agtgaagtga atgaccattc
     2521 cgtcttcgag acaggggatc agcttctcga ggaacgccct gtacccaact gccgggaagc
     2581 ttatgataac gatccctgcc ccctcgacag ctttcctcat gtcgtcggtg accacgtcta
     2641 ttttggcaag cccctctcgc ttgaacccgt acaggctttc ctgttttccg ctgacgcgga
     2701 tctccctgtt cttcgctata cacccgaggc cgtcaaaaaa aacgggaagc tcgtacagcc
     2761 gaacctccct gccggcaagg gccgtgcacg cggcatgtcc cctggccgtg gctccgcctc
     2821 caaggaccgc gacgggcttg ttccccaggt actccattcc agccataaca taacctccta
     2881 aagtctgccg ccgagcacaa gccgtggaag ccagagtgct agctccggaa tgtacgtgac
     2941 agccagaagt gtgggaagcc aggcaaaaag gataaatatc attgccggct tcatcatctg
     3001 ggagacgggc gtcttcgcca tccgcccgcc gaaatagagc gtgggcgcag tgggtggagt
     3061 cacgtttccg agtcccaggt tggttcctat gatcgccgcg aagtggatcg ggtgtattcc
     3121 gatggacacc acgacgggaa gcagaatggg cgtacagagc agcatggcgc tcacgtcatc
     3181 cattatcatt ccgatgatga tcatgaaaat attgatcata agcatgatca cgtacttgtt
     3241 gctggaaatc gccgtgagaa agtctatgat catggcagga agccgctcct ggacgtaaat
     3301 ccggctcagc atcatgatgc agtacatcat gagcataacg acccctgtgg tcgtcgcggt
     3361 ctcgatgatc acctctaaaa aattgttccg gttcagcccc ctgtagatca ggaatcccac
     3421 cggtatggag tacagaacgg caaccgcggc cgcttcggtg ggggtcatga tgccgccgta
     3481 aatcccgccg agcacgatca ccggcatgaa aagggcggga accgcctttt tcccccggct
     3541 tccgagaagt tcgatttttt cctggaaggt gatctgcggc gccacgttta cattgggatc
     3601 cttcctcagc ataacgaggt tcagaatgct gagaagaatc atgagcacta tcccgggacc
     3661 gacggtggca agaaagcacg caagcaccga ctgctgcccc acccacgcga acaggatcat
     3721 ctgggagctc ggggggatca gcagccccag gggggccgcg caggacacca gggcggcgct
     3781 gtgacccatg ggatagccgt tctcccggag gcggggaaac atgatggagc cgatgcacga
     3841 aagggtggcg gcgcagcttc cggagatgga gccgaagacc gcgc
//
LOCUS       DAOTHD010000074         3882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_27737_length_3882_cov_2.707604, whole genome shotgun sequence.
ACCESSION   DAOTHD010000074 DAOTHD010000000
VERSION     DAOTHD010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27737_length_3882_cov_2.707604"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="P5219_02095"
     CDS             complement(<1..184)
                     /locus_tag="P5219_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRX25577.1"
                     /translation="MITLKKENELVYMRKAGKIVADVLDMIGGIVRPGVTTAEIDRAA
                     EDIIRKAGATPAFKGYR"
     gene            complement(181..831)
                     /locus_tag="P5219_02100"
     CDS             complement(181..831)
                     /locus_tag="P5219_02100"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRX25578.1"
                     /translation="MRIILLGSPGAGKGTLAAEIDKKYPVAHISTGDIFRENVKNNTP
                     LGLKARSYMDAGKLVPDDIVIAMMEDRLREPDCEKGFILDGFPRTVPQAKALEKILEE
                     LHLSLDKVVLLDVDEETVVKRLSGRRTCRSCGAIYNVEFKPSAKGDLCEKCGGELYQR
                     DDDREDVIRKRLRVFYDQTAPVISYYEGKHKFVRLPSARHGHSLLEELETAVGGTE"
     gene            complement(843..2135)
                     /gene="secY"
                     /locus_tag="P5219_02105"
     CDS             complement(843..2135)
                     /gene="secY"
                     /locus_tag="P5219_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRX25579.1"
                     /translation="MIDSFRDAFRLPDLKRRILFSLGVLFIFRLGAHVPTPGIDAEAM
                     SRLFDGGGVLGFLDIFAGGALRRFSIFALGVAPYINASIVMQLLVVIFPALEKMQKEG
                     EDGRKKIVQWTRYAAVLFALVQAVGMTFWLRGLGIFSGHFFDGVVVVSTVTAGSIAVM
                     WLGEEISDHGIGNGISLLIFAGIVARVPEAIIQSWNMLKLGEMNALVLLLALMLMVGV
                     IAGCIMLQEGQRRLPVQYAKRVVGNKVYGGQSTFIPLKVNQAGVIPIIFASSVLLFPY
                     SVAKFFTGDIGLFFQRIFAPDSIFYTVFYVALIVFFSYFYTAVVFNPSDVANNMKKYG
                     GFILGIRPGKPTSDFIEKVMSRITLGGAVFLAGVALVPNLMTQFMGITSFYFGGTAVL
                     IVVGVALDTVHQIEGQLLMRHYEGILKRKDKAGGLLRF"
     gene            complement(2136..2582)
                     /gene="rplO"
                     /locus_tag="P5219_02110"
     CDS             complement(2136..2582)
                     /gene="rplO"
                     /locus_tag="P5219_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRX25580.1"
                     /translation="MSLHELRPAPGAKRKGKRLGQGIGSGTGKTAGKGHKGQKARSGG
                     GVRPGFEGGQMPMMRRIPKKGFNNARFAKEFQIVNVESLASRFEAGATVGFSEMYAAR
                     LVQKDNVPVKVLASGDIDKALTVQAQAFSAQAKAKIEAAGGKAEVI"
     gene            complement(2595..2780)
                     /gene="rpmD"
                     /locus_tag="P5219_02115"
     CDS             complement(2595..2780)
                     /gene="rpmD"
                     /locus_tag="P5219_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRX25581.1"
                     /translation="MAKLRITWKRSSIGRPEVQKRTVRALGLTKLNQTVEHDDTPQIR
                     GMVNKVSHLVECVAVEN"
     gene            complement(2792..3304)
                     /gene="rpsE"
                     /locus_tag="P5219_02120"
     CDS             complement(2792..3304)
                     /gene="rpsE"
                     /locus_tag="P5219_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRX25582.1"
                     /translation="MIRKKIDAKGMELTERVVAINRVSKVVKGGKRFKFSVLVVVGDG
                     DRYAGVGMGKAKEISEAVRKGIDKAGKSLVELKRIGNTIPHPVNGVFGAASVLLKPAV
                     PGTGVIAGGVVRAIMELGGVKDVVAKVIGRTSNPINVAWATVQALEEMRTPDEILRLR
                     GKKSPEAAAE"
     gene            complement(3320..3661)
                     /gene="rplR"
                     /locus_tag="P5219_02125"
     CDS             complement(3320..3661)
                     /gene="rplR"
                     /locus_tag="P5219_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRX25583.1"
                     /translation="MREIRHARLRKQVSGTAAKPRMAVFHSLSHIYVQFIDDEAGHTL
                     AAASTVEPSVKSVLAGTCNMEAAKAVGKVAAERALAKGITSVVFDRGGHMFHGKVKAL
                     AEAAREAGLKF"
     gene            complement(3702..>3882)
                     /gene="rplF"
                     /locus_tag="P5219_02130"
     CDS             complement(3702..>3882)
                     /gene="rplF"
                     /locus_tag="P5219_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRX25584.1"
                     /translation="IEIALDGPTKLSVAGIDKQAVGQVAAIIRGFRSPEPYQGKGIRY
                     AGERIIRKAGKTGAK"
BASE COUNT          721 a         1204 c         1098 g          859 t
ORIGIN      
        1 cccggtatcc cttgaaagcg ggggtcgccc cggccttccg gatgatgtcc tcggccgccc
       61 ggtcgatctc ggcggtggtc actccggggc gaacaatccc gccgatcatg tcaagcacat
      121 cggctacgat ctttcccgct ttccgcatgt acaccagttc gttttctttt ttcagtgtta
      181 tcattccgta ccgccgactg ccgtctcgag ttcctcgagc agtgaatgtc cgtgccgcgc
      241 cgagggcagc cttacgaact tgtgctttcc ttcgtaatag gatatcaccg gcgcggtctg
      301 gtcataaaat accctcagcc gtttgcggat gacatcttcc ctgtcgtcat cccgctggta
      361 gagttctccg ccgcattttt cgcaaaggtc tcccttcgcc gagggcttga actccacgtt
      421 gtagatcgct ccgcaggagc ggcaggtcct ccgccccgag aggcgcttga ccaccgtttc
      481 ttcgtcgaca tcgaggagca ccactttgtc gaggctgagg tgcaactctt caaggatctt
      541 ctccagggct ttcgcctggg gaaccgtccg gggaaaacca tcgaggatga aacccttttc
      601 gcagtcgggc tcccgaagcc gatcctccat catcgcaatg acgatgtcgt caggcacgag
      661 cttgcccgca tccatgtagg accgtgcctt caggcccagc ggagtattgt ttttcacgtt
      721 ttcgcgaaaa atatctccgg tggaaatatg cgctacaggg tatttcttgt cgatttccgc
      781 cgctagcgtc cctttgcccg ccccgggtga accaagaaga attatgcgca ttgtccttct
      841 ccttaaaagc gaagtagtcc gccggccttg tccttacgct tgagtatccc ctcgtaatgc
      901 cgcataagaa gctgtccctc gatctggtgg accgtgtcga gagccacgcc gacgacgatg
      961 agaaccgctg ttccgccgaa gtagaagctg gtaatcccca tgaactgggt catgaggttc
     1021 ggaacaaggg cgacccctgc gaggaacaca gcaccgccca gggtgatccg gctcatcact
     1081 ttctcgatga aatccgatgt gggtttgcca ggccggattc cgagaatgaa gcctccgtac
     1141 ttcttcatgt tgttcgccac gtctgacggg ttgaagacca cggcagtgta gaagtagctg
     1201 aagaagacga tgagcgccac gtagaaaacg gtgtagaaga tgctgtccgg cgcgaaaatg
     1261 cgctggaaga acaggcctat gtcaccggtg aaaaactttg ccaccgaata ggggaacagg
     1321 agcaccgatg aggcgaagat gatggggatg actccggcct ggttgacctt caggggtatg
     1381 aaggtgctct gtccgccgta gaccttgttg cctaccaccc ttttggcgta ctgcacgggg
     1441 agccgtctct gcccctcctg cagcatgatg catcccgcta tgacgcccac catgagcata
     1501 agggcgagca gcaggacgag ggcgttcatt tcgccgagct tgagcatgtt ccagctctgg
     1561 atgatggcct cgggaaccct ggcgacgata ccggcgaaga tgagcaggga aatgccgttg
     1621 ccgatgccgt ggtccgagat ttcctcgccg agccacatga cggcgatgga tcccgccgtg
     1681 acggtgctca cgacaaccac tccgtcgaag aagtgaccgc tgaatatacc gagacctcga
     1741 agccagaagg tcatgcccac cgcctgcaca agggcgaaga ggactgcggc gtaccgggtc
     1801 cactggacga tcttcttccg gccgtcctcc ccttccttct gcatcttctc aagggcgggg
     1861 aaaatgacca caagcagctg catgacgatg ctggcgttga tgtacggggc gacgccgagt
     1921 gcgaatatgc tgaaccgcct cagcgcgccg cccgcgaaaa tgtcgaggaa tccaaggact
     1981 cccccgccgt cgaacaggcg ggacatggcc tcagcgtcga tcccgggcgt cgggacgtgg
     2041 gcccccaggc ggaaaatgaa cagcaccccg agggagaaga ggattcttct tttcaggtca
     2101 ggcagtctga aggcatcccg gaaggaatcg atcacttaga tcacctcggc cttcccaccc
     2161 gctgcttcga tcttcgcttt tgcctgggcg ctgaacgcct gggcctgcac agtgagcgcc
     2221 ttgtcgatgt cgcccgaggc gagaaccttc acggggacat tgtccttctg gacgaggcgc
     2281 gctgcataca tttcggagaa gcccaccgtc gcccccgctt caaatctgga ggcaagggat
     2341 tccacgttga cgatctggaa ttccttggcg aatctcgcgt tgttgaaacc cttcttggga
     2401 atccggcgca tcatgggcat ctggccgcct tcgaagcccg gccgcactcc gccgccgctc
     2461 ctggctttct gtcccttatg accttttccc gcagtctttc ccgttccgct tccgatgccc
     2521 tgcccgagcc gtttgccttt tctcttggct ccgggggcgg ggcgaagttc atgcaagctc
     2581 atggagtgtc ccccctaatt ctccacggca acacattcca ccaggtggct gaccttgttg
     2641 accatgccgc ggatctgggg ggtgtcgtcg tgttctactg tctggttgag tttggtgagg
     2701 ccaagggcac ggactgtccg cttctggacc tcgggcctgc cgatggagct cctcttccaa
     2761 gtgatacgaa gctttgccat gagaatgcct cctactcagc agcggcttca gggcttttct
     2821 tgcccctgag ccggagaatc tcgtcgggag tcctcatctc ctcaagagcc tggacggtcg
     2881 cccaggctac gttgatgggg ttggacgtcc tgccgatgac cttggccact acgtccttca
     2941 ctcccccgag ctccatgatg gcacggacca ctccgccggc gataactccc gttccgggaa
     3001 cggcaggctt gagaagaacg gaagccgcgc cgaaaacgcc gtttacgggg tggggaatgg
     3061 tgttcccgat cctcttcagt tccacgaggc tctttccggc cttgtcaatg cccttgcgga
     3121 cagcttcgga aatttccttc gccttgccca tgccgacacc ggcgtaccgg tcgccgtcgc
     3181 ctactacgac gaggacgctg aacttgaagc gctttccgcc cttgacaacc ttgctgaccc
     3241 ggttgatggc gacgacgcgc tcggtaagtt ccatcccctt ggcatctatc tttttcctga
     3301 tcatcctctg cgcctcccct tagaacttca ggccggcttc gcgggcggct tccgccaggg
     3361 ccttgacctt gccatggaac atgtgtccgc ccctgtcgaa gaccaccgac gtgatgccct
     3421 tggcgagagc acgttcagca gcgactttgc ccaccgcctt ggcggcttcc atgttgcacg
     3481 taccggccag gacgctcttg acggacggct ccaccgtgga agccgccgcg agggtatgcc
     3541 cggcttcatc atcgatgaac tggacgtaga tgtgggacag gctgtggaaa acagccatcc
     3601 tgggcttggc ggcggtgccg gacacctgtt tgcgaagcct ggcgtgacga atttcgcgca
     3661 tgacgcttct gcttttgatc ttcatgtcaa ttcacctcaa attacttggc gccggtcttt
     3721 ccggccttgc ggataatccg ctcgccggcg taccgtattc ccttgccctg gtaaggttcg
     3781 ggagagcgga acccgcgaat gatcgccgct acctggccca ccgcctgctt gtcgatcccc
     3841 gccacagaaa gtttcgtggg accgtcgagg gcgatttcaa tc
//
LOCUS       DAOTHD010000075         3882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_27738_length_3882_cov_2.699504, whole genome shotgun sequence.
ACCESSION   DAOTHD010000075 DAOTHD010000000
VERSION     DAOTHD010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27738_length_3882_cov_2.699504"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="P5219_02135"
     CDS             <1..237
                     /locus_tag="P5219_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRX25585.1"
                     /translation="ADKPLKYPYLFSSSKIVLLTKTDLLPYLPFDMKMFTDDVKSLNP
                     EAPVLHLNGLTGEGFGGWTDFLKDRIKEKTADAR"
     gene            227..2206
                     /locus_tag="P5219_02140"
     CDS             227..2206
                     /locus_tag="P5219_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRX25586.1"
                     /translation="MPDEKKLLEKLNPRQREAVAYCDGPLLVLAGAGSGKTRVLAHKI
                     AYLVAKGLSRPEDILAVTFTNKAANEMKERVRALLGDTGREMQICTFHSFGLRFLFRN
                     RAFLPSVGLKEGFAIFDRGDSRSLVKEIMEGLNIDTKQFEPSSMLDRISTVKNDCLPA
                     GRSSPLLEGIYGDVFTAYNEALRRQNAVDFDDLLLLPLRLLDSEPDLRERERNRLSWI
                     LVDEYQDVNRPQYLTLGKLVGPKRKIMVVGDPDQSIYGWRGADMTMILNFEQDFPSSK
                     VVILDQNYRSSGTILGAANALIEGNSKRRKKDLWTARNMGEKVYTLLGQTEYQEADFL
                     MSEIRRLHDREGYSYGDMALLYRINAMSRVYEQKFLENRIPYRVVRGTAFYDRKEVKD
                     ILSFLRAAVNPLDRVSLTRIANVPARGLGKKSLEKVFAFLESLPALSAGEYWKTLAEG
                     VRAAEGKAGTGLKDLARHMVKILSLSDSLADILTYILDTMGYEDILRKDDPDGWEERV
                     ENIRELRSIVPTGGDLSEMLAEAALFTDLEKLDLDDKNAVNLLTLHAAKGLEFPVVFL
                     VGMEEAVFPHFKCLEDRENMEEERRLCYVGMTRAEERLYMTGVRSRRLFGTTFRNGFS
                     RFLWEIPDKFKTVDDRGEEEKQHAGFGSNRRRWGW"
     gene            2166..3029
                     /gene="coaE"
                     /locus_tag="P5219_02145"
     CDS             2166..3029
                     /gene="coaE"
                     /locus_tag="P5219_02145"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRX25587.1"
                     /translation="MLALGVTGDVGAGKSTLTRFWKEMGASVLDADEIVRSLWKRPHV
                     IGEAVSRWGRGVADTGGNILPGEIASRAFSSAEDYAWLCGLLHPLVRIEMERMSASLE
                     GWVVAEIPLLFEGGVPQWIDATVYLAAPEEKRRERNSSRGWTAGEIIRRESFLLPSEE
                     KKRRADVVLENAGTLENLRETAEKHALFFRRLAGLVRCTVTFQRYDDALLYRDFLRKE
                     RLGTDFDCLRVSSMDKNAEGWLVSFISFEHLFAGLSRPHVMDGARGPYMAAIRRMPHK
                     RRIALSEELTQ"
     gene            3026..>3882
                     /locus_tag="P5219_02150"
     CDS             3026..>3882
                     /locus_tag="P5219_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25588.1"
                     /translation="MKVITSHMGNDFDSLASMVAAQKLYPGARLCLSGSASRTVREFL
                     KKHGSRWSVATPKKIKFDEITMLVVVDARSRSRIGPFASLLGKKNIPVHVYNHHPPST
                     DDIDAEFIAIEPVGATTTLLVEILLERRIPISSSEATLFATGIYEDTGGLTFSGTTPR
                     DFAAVARMKELGADLTSIPTFIEMTFSAPERKILDSLIENSWIRFINGAKAVFSAVSS
                     PGYVDGLSLFVHRLRDYFDADIAIAAVRMDTRTYLIGRSHDDVLDMAAFLSPLGGGGH
                     PQAASVTVH"
BASE COUNT          887 a         1126 c         1110 g          759 t
ORIGIN      
        1 gcggacaagc cgctcaaata cccttacctt ttcagttcat cgaagatcgt tcttctgacc
       61 aagacagacc tccttccgta cctccccttc gacatgaaga tgttcacaga cgatgtgaag
      121 tccctcaatc cagaggcacc tgttctgcac ctcaacgggc ttaccgggga aggattcggg
      181 ggctggacgg actttctgaa agacaggatc aaggagaaaa cagcggatgc ccgatgagaa
      241 aaaactcctg gaaaaactga atccccggca gagggaagcg gttgcctact gcgacggccc
      301 ccttctcgtc ctggcgggcg ccggcagcgg caagacaagg gtccttgccc acaaaatcgc
      361 ctatctcgtg gcaaagggcc tttccagacc cgaggatatt cttgccgtga cgttcaccaa
      421 caaggcggcc aacgagatga aggaacgggt ccgggcgctt ctcggggaca cgggacggga
      481 gatgcagatc tgtacattcc actccttcgg ccttcgcttt cttttccgga acagggcctt
      541 tctcccctca gtcggcctaa aggaaggctt cgccattttc gacaggggtg acagccgttc
      601 cctggtcaag gaaatcatgg aaggactgaa catcgacacg aagcagttcg agccttcgtc
      661 catgctcgac cgcatatcca cggtaaagaa cgactgcctc ccggcgggga ggagttcccc
      721 cctgctcgag ggcatctacg gcgatgtctt caccgcctat aacgaagcac tgagacggca
      781 gaacgccgtc gactttgacg atctgcttct gcttcccctc cgtctgctgg attcggaacc
      841 ggatctgcgg gaaagggagc ggaaccgcct ttcgtggatc ctggtggacg aataccagga
      901 cgtcaacagg ccccaatatc tcaccctcgg caagctggtg ggaccgaagc ggaaaataat
      961 ggtggtgggg gatccggacc agtcgattta cggctggcgg ggagccgaca tgaccatgat
     1021 cctcaacttc gaacaggatt tcccttcgtc aaaggtcgtc attcttgacc agaattaccg
     1081 ctcctccggc acaattctcg gggcggcaaa cgccctgatc gaaggcaatt caaagaggcg
     1141 taaaaaagac ctgtggaccg cccgcaacat gggggagaaa gtctacaccc tcctcgggca
     1201 gacggagtac caggaggcgg atttcctgat gtcggaaata cggaggctcc atgacaggga
     1261 aggatacagc tacggcgata tggccctcct gtaccgcatc aacgccatga gccgggtcta
     1321 cgaacagaaa ttcctcgaaa accggatccc ctaccgggtt gttcggggaa cagccttcta
     1381 cgaccggaag gaagtaaagg atatcctctc cttcctcagg gcggcggtga accccctgga
     1441 cagggtgtcc cttacccgaa tcgccaatgt cccggcaaga gggctcggaa agaagagcct
     1501 ggaaaaggtg ttcgcctttt tggaatccct gccggctctt tccgccgggg agtactggaa
     1561 gacccttgcg gaaggggtca gggcggcgga aggaaaggca gggacagggc tgaaagacct
     1621 ggcacggcac atggtgaaga tcctctccct ttccgacagc ctggctgaca tacttaccta
     1681 cattctcgac accatgggat acgaggacat cctcaggaaa gatgatcccg acgggtggga
     1741 agagagggtg gaaaacatca gggaactccg ctccatagtc cccacgggcg gcgacctctc
     1801 cgaaatgctc gccgaggcgg cccttttcac ggaccttgaa aaactcgacc tggacgacaa
     1861 gaatgcggtg aacctcctga ccctccatgc tgcgaaaggg ctcgaatttc ccgtggtctt
     1921 tcttgtggga atggaggagg ccgtctttcc tcatttcaaa tgcctggaag acagggagaa
     1981 catggaagag gagcggcggc tctgctacgt gggcatgacc agggccgaag aaaggctcta
     2041 catgaccgga gtcagatccc gaaggctctt cggcaccaca tttcgcaacg gcttttcccg
     2101 ttttctctgg gaaatccccg acaagttcaa aacagtggac gacaggggcg aggaggaaaa
     2161 acagcatgct ggctttggga gtaacaggag acgttggggc tggtaaatcc acgctgaccc
     2221 gtttctggaa ggaaatgggg gcatccgtcc tggatgcgga cgaaatcgtc cggagcctgt
     2281 ggaagcgccc tcatgtcatc ggcgaggcag tctcacgatg gggcagggga gtggcggata
     2341 ccggggggaa catcctgccg ggagaaatag catcccgggc cttcagttcc gctgaagatt
     2401 acgcatggct ctgcgggctc cttcaccctc tcgtccggat tgaaatggaa cgtatgtccg
     2461 cctccctgga aggctgggtg gtggccgaaa taccccttct tttcgaaggc ggagtgcccc
     2521 agtggatcga tgctacggtc taccttgccg cacctgaaga aaagagaagg gaacgaaact
     2581 cttcaagagg atggactgca ggcgagatca tcaggaggga atccttcctt ctcccttcgg
     2641 aagaaaaaaa gcggcgggcg gatgtggtgc tcgaaaatgc cggcaccctc gagaacctca
     2701 gggagacggc ggaaaagcat gctctctttt tccgccgcct tgcaggcctt gtccggtgca
     2761 ccgtgacctt ccagcgatac gatgatgccc ttctgtacag ggattttctc aggaaggaac
     2821 ggctcgggac agattttgac tgcctcaggg tcagctccat ggataaaaat gccgaaggtt
     2881 ggctcgtctc gtttatttct tttgaacacc tttttgccgg tctttcccgc ccccatgtaa
     2941 tggacggagc caggggaccc tacatggccg ccatacggcg gatgccccat aaaaggcgca
     3001 ttgccctttc ggaggaactt acgcagtgaa ggtcatcacc agccacatgg gcaacgattt
     3061 tgactcgctg gcaagtatgg tggcggccca gaagctttat cccggcgcca ggctctgcct
     3121 gtcaggttcc gcaagccgca cggtgaggga gttcctcaaa aagcacggca gcaggtggtc
     3181 cgtggcgact ccgaagaaga tcaagtttga cgagatcacc atgctcgtcg tggtggacgc
     3241 gaggtcacgt tcgagaatcg gcccctttgc atcccttctc gggaaaaaga acatcccggt
     3301 tcacgtgtac aaccaccacc ccccctccac cgacgatatc gacgccgagt tcatcgccat
     3361 cgaaccggtg ggagcgacca caactctcct cgtggaaatt ctcctcgaaa ggagaatccc
     3421 catctcctcc agcgaggcga ccctcttcgc caccggcatt tacgaggaca ccggcggcct
     3481 caccttcagc ggcacaaccc cccgggactt cgcggccgtt gcgaggatga aggaactcgg
     3541 ggccgacctt acgagcatac caacgttcat tgaaatgacc ttctccgctc cggagaggaa
     3601 aattctcgac agcctcattg aaaacagctg gatcaggttc atcaacggcg caaaagccgt
     3661 tttttccgca gtctcctccc caggatacgt ggacggcctt tccctgttcg tccacaggct
     3721 gagggactat ttcgacgccg atatcgccat cgccgcggtc cggatggata cccggaccta
     3781 cctgatcggg cggagccatg acgatgtcct tgacatggcg gctttccttt cacccctcgg
     3841 aggcggcggc catccccagg cggcttcggt caccgttcac aa
//
LOCUS       DAOTHD010000076         3867 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_27879_length_3867_cov_3.304827, whole genome shotgun sequence.
ACCESSION   DAOTHD010000076 DAOTHD010000000
VERSION     DAOTHD010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27879_length_3867_cov_3.304827"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="P5219_02155"
     CDS             complement(<1..693)
                     /locus_tag="P5219_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX25589.1"
                     /translation="MLKIEDLHVYYGAIHAVKGISLSIPKGSIVTLIGANGAGKSSTI
                     RAVAGLVRNVKGKIVYTPPEGEPVSLVGKAPEEMVKLGIAMSPEGRRILPHLTVEENL
                     QLGAFIRNDKDGIQRDRDHVYDLFPRLKERSWQKGGTLSGGEQQMLAVGRALMSQPHL
                     VMLDEPSLGLAPLLVKEVFDIIGEINSRGKTVLLVEQNAFAALKIASYAYILEVGAIV
                     LEGKGEDLLKDSR"
     gene            complement(680..1474)
                     /locus_tag="P5219_02160"
     CDS             complement(680..1474)
                     /locus_tag="P5219_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX25590.1"
                     /translation="MTNPEPILRTENVTMRFGGLTAVRDFSIDVPRGSIVGLIGPNGA
                     GKTTVFNIVTGFYKPTEGKVLFDGQDITGIPPHMVCRAGISRTFQNIRLFASESVLEN
                     VMVGCHVRRKAKWWMAPLGLPLFRREEEEIREKSMKLLETVGLAQYADENSGSLPYGA
                     QRRLEIARALATDPKFLLLDEPAAGMNPQETRELMGFIRNIRERFGITILLIEHDMKV
                     VMGVCEYIWVLDYGVKIAEGGPDAIQSDQKVIVAYLGEEYVKYAEN"
     gene            complement(1471..2514)
                     /locus_tag="P5219_02165"
     CDS             complement(1471..2514)
                     /locus_tag="P5219_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRX25591.1"
                     /translation="MNRTRNLILNCLAVAGLGAFIWWAQDNLDGYQIQVLNLIAVNAI
                     LALSLNLIYGFTGMFSLGHAGFMAIGAYVCALLILTPAQKETMWILEPLVWPLSVIQS
                     PFFVAVLLGGLIAALCGLIIAIPVLRLGGDYLGIATLGFAEIIRVVLTNLSFITNGAL
                     GIKGIPNYANLWWNYGWLLLTLFVIVRLVNSNFGNVLKAIRDDEIAAKNMGINTFFYR
                     VVSFSVGAFFAGVGGALMGSLITTIDPKMFVFTLTFNVLMITVAGGLGSITGSLIGSV
                     VITVLLEWLRIVENPLVIGDFEIPGIPGMRMVIFSLALILIILFRSEGIMGMREFSWN
                     RLFGRSAQGGDAK"
     gene            complement(2511..3404)
                     /locus_tag="P5219_02170"
     CDS             complement(2511..3404)
                     /locus_tag="P5219_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRX25592.1"
                     /translation="MSGSMFFQHLFNAITLGSLYGLIAIGYTMVYGILRLINFAHGEV
                     FMLGAYFIFFAITLLNLPWGLAAVLAVAASSFCGILVDKIAYKPLRNAPRISALISAI
                     GVSFFIQNLSIVIFTGLPRPVMRPDWLVKVMKFGEIRILPLAIVVPAVTLFLVLGLLW
                     IVYRTKPGLAMRAISKDIETTRLMGVSVNRIIAFTFGIGSALAAASGIMWALRYPQIH
                     PFMGLFPGLKAFIAAVFGGIGSIQGAVIGGMFLGIIEIMAVAFFPTLSGYRDAFAFVL
                     LIIILLMKPTGLMGEKLEDKI"
     gene            complement(3501..>3867)
                     /locus_tag="P5219_02175"
     CDS             complement(3501..>3867)
                     /locus_tag="P5219_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRX25593.1"
                     /translation="IGGSAVEGFMHTTFPYDPSMTGMSDIAKSFTENWKKAHPDKDPN
                     VNAALGYDSYMLVANAIKVAGSTEPDAIRDALAKTKGFPGVTGNKTINETHDAESPVG
                     IVQIKDGKKQFIGTVEPEM"
BASE COUNT          779 a         1091 c         1234 g          763 t
ORIGIN      
        1 cctgctgtcc ttcagcaggt cctcgccttt cccttcaagg acgatggccc cgacttccag
       61 aatgtaggcg taggacgcga ttttcagggc tgcaaaggcg ttctgctcca ccaggagcac
      121 tgtttttccc cgggaattga tctccccgat gatgtcgaag acctccttga cgagcagggg
      181 ggccagtccc agggagggct cgtcgagcat caccaggtgg ggctggctca tgagggcccg
      241 tcccacggcc agcatctgct gctcccctcc ggaaagagtg ccccctttct gccaggaacg
      301 ctccttcagc cgggggaaga gatcgtagac gtgatccctg tcccgttgaa ttccgtcctt
      361 gtcgttcctg atgaaggcac cgagctggag gttctcctcc acggtgaggt ggggcaggat
      421 gcgccgaccc tcggggctca tggcgatacc gagcttcacc atctcctcgg gtgcctttcc
      481 gacgaggctc accggctccc cttcgggcgg ggtgtagacg atcttcccct tgacgttccg
      541 caccagccca gccacggcgc ggatggtgct gctttttccg gccccgttgg cgccgatgag
      601 ggtcacgatg cttccctttg gaatggagag agaaattccc ttgacggcgt gaatggcccc
      661 gtagtagacg tggagatctt caattttcag catatttcac gtactcctct ccgaggtagg
      721 ccacgatcac tttctggtcc gactggattg cgtcggggcc gccttcggcg atcttcacgc
      781 cgtagtccag gacccagatg tactcgcaga ctcccatgac taccttcatg tcatgctcga
      841 taagcaggat ggtgatgccg aaccgttccc ggatgttccg gatgaatccc atcagctccc
      901 tggtctcctg gggattcatt cctgccgcgg gctcgtccag gagaaggaac ttcgggtccg
      961 tggcgagggc cctggcaatc tccaggcgac gctgggcacc gtagggaagg gagccggagt
     1021 tttcatccgc atactgggcg agccccacgg tttcgaggag cttcatggat ttttcccgga
     1081 tttcctcctc ctccctgcgg aagagaggaa gtcccagggg cgccatccac catttcgcct
     1141 tccttcggac gtggcacccg accatgacgt tctccagcac cgattcgctg gcgaagagcc
     1201 gtatgttctg gaacgtccgg gaaatgcccg cccggcacac catgtgggga gggatgccgg
     1261 tgatgtcctg gccgtcgaag agaaccttcc cctccgtggg cttgtagaag ccggtgacga
     1321 tgttgaacac cgtggtcttg cccgccccgt tgggaccgat gaggcccacg atggagcctc
     1381 gggggacgtc gatggagaaa tctctgacgg cggtgagacc gccgaacctc atggtgacgt
     1441 tttccgtcct gagaatgggt tcaggattgg tcatttcgcg tcacctccct gggcggaacg
     1501 gccgaacagc ctgttccagc tgaactccct catgcccatg atgccctcgc tcctgaagag
     1561 gatgatgagg atgagcgcca gggagaaaat aaccattctc atccccggaa tgcccggtat
     1621 ttcaaagtct ccgatcacca gggggttttc cacgatccgg agccactcca ggaggaccgt
     1681 gatgaccacg ctcccgataa ggcttcccgt gatggacccc aggccgccgg ccacggtgat
     1741 catcaggacg ttgaacgtca gggtgaagac gaacatcttc gggtcgatgg tggtgatgag
     1801 gctgcccatg agggctcctc ccactccggc gaagaaggcc cctaccgaaa aggagaccac
     1861 cctgtagaag aaggtgttga tgcccatgtt cttcgcggcg atctcgtcgt cccggatggc
     1921 tttcagcacg ttgccgaagt tgctgttgac gagcctgacg ataacgaaaa gggtcaggag
     1981 aagccacccg tagttccacc acaggttcgc atagttgggg atgcccttga ttcccagggc
     2041 tccgttggtg atgaagctga ggttcgtgag gaccacccgg atgatctcgg cgaagcccag
     2101 ggtcgcgatg ccgagatagt cgccgccgag ccggagcacc ggtatggcga tgatcaggcc
     2161 gcagagggcc gctatgagcc ctcccaggag caccgccacg aagaaggggg actggatcac
     2221 cgaaaggggc cagacgagag gctcgaggat ccacatggtt tccttctggg cgggggtgag
     2281 gatgaggagg gcgcacacat aggcgcctat ggccatgaac cccgcgtggc cgagggagaa
     2341 catgccggtg aagccgtaga tcaggttgag gctgagggcg agaatggcgt ttactgcgat
     2401 gaggttgagc acctggatct ggtacccgtc aagattgtcc tgggcccacc agatgaacgc
     2461 ccccagcccc gcgacggcca ggcagttgag gatgagattt ctcgtccggt tcatatcttg
     2521 tcctccagct tctcccccat aagcccagtg ggcttcatga gcaggatgat gatgagaagt
     2581 acgaaggcga aggcgtcacg gtaccccgac agggtgggaa aaaaggccac cgccatgatt
     2641 tcgatgatgc cgaggaacat gccgccgatg accgctccct ggatggagcc gatgccgccg
     2701 aaaacggccg cgatgaaggc tttcaggccg gggaaaaggc ccatgaaggg gtggatctgg
     2761 ggatagcgca gggcccacat aatgcccgac gcggcggcaa gggccgagcc gattccgaag
     2821 gtgaaggcga tgatcctgtt caccgacacg cccatgaggc gagtggtttc gatgtccttc
     2881 gagatggccc tcatggcaag ccccggcttg gtccggtaca cgatccagag gagaccgagc
     2941 accaggaaga gggtcacggc ggggacgact atggcaaggg gaaggatgcg gatctcgccg
     3001 aatttcatga ccttcacgag ccaatcgggc ctcattacgg ggcggggaag gccggtgaag
     3061 atcacgatgc tgagattctg gataaaaaag gatacgccga tggcgctgat gagggcggag
     3121 atccgcggag cgttccggag aggcttgtag gctattttgt ccaccaggat gccgcagaag
     3181 gagctggcgg ccaccgccag gacggcggca agtccccagg ggaggttcag cagggtgatg
     3241 gcgaagaaga taaagtaggc gcccagcatg aagacttctc cgtgggcgaa gttgatgagc
     3301 ctgagaatac cgtagaccat ggtgtagccg atggcgatga gaccgtagag agatccgagt
     3361 gttattgcgt tgaaaagatg ctggaaaaac atgcttccgc tcattaaggt tccccctgac
     3421 ttcaagaaaa tggagggtgg aggcgttccc ccaccctccg gaaaaagagg cgcaaacgaa
     3481 ccttgaggaa gggacctccc ttacatttcg ggttcgacgg ttccgatgaa ctgcttcttt
     3541 ccgtccttga tctgcacgat gccgacaggg ctctcggcgt cgtgggtctc gttgatggtc
     3601 ttgttgccgg tcacgccggg gaaacccttc gtcttggcaa gagcgtcgcg gatggcatcg
     3661 ggctcggtgg acccggccac cttgatggcg ttggccacaa gcatgtagga gtcatagccg
     3721 agagcggcgt tgacgttggg atccttgtcg gggtgcgctt tcttccagtt ctcggtgaag
     3781 gacttggcaa tgtcgctcat gccggtcatg gaaggatcgt aggggaaggt ggtgtgcatg
     3841 aagccttcca cagcagagcc gccgatg
//
LOCUS       DAOTHD010000077         3866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_27893_length_3866_cov_3.340593, whole genome shotgun sequence.
ACCESSION   DAOTHD010000077 DAOTHD010000000
VERSION     DAOTHD010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27893_length_3866_cov_3.340593"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="P5219_02180"
     CDS             complement(<1..993)
                     /locus_tag="P5219_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRX25594.1"
                     /translation="MIHCRPFSRSSAGGADPSGRIPPRSEIPGEYTWDLDAIFPSSEA
                     WEESCRVLGEELERLGGFNGRLFESPETLLEFLKLEEAASERMGKIYAYAVMKSHEDT
                     ADAARQARADRAGTLLASYGAVLSFYRPEVLAAGAEGVKAAAEGCPGLEMYSHHFDDL
                     LRTAGHVLGLQEEEILARSGEMARTPENAFSLLTNADLEFPEILDESGRETELSEERY
                     YLLSRSKDRRVRKDAFEGLFSTYGKFRNTLAALYSGSVKSDLFFSRARKYPSALEASL
                     HGDNISPSVYAMVVDTVNRNLDALHGYMALRKKALGLEELRMYDLNVPLADEPET"
     gene            1136..2143
                     /locus_tag="P5219_02185"
     CDS             1136..2143
                     /locus_tag="P5219_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRX25595.1"
                     /translation="MKKRVLVLAALALIFALSGSAVSAQTVKPDQLRFMAGPPGGNWF
                     ALGGALADLWTSKLIPTTSITGGAVANIINTHNAKGELGFSNTSMVAVGQKASDAPFK
                     DPTGNTLIMANLYTQYTYFIARKDFVEKHGITSLDDLVTKKIPTRFATLKTGTGSEFI
                     VNGVFKAGFGIADYRKELKDWGGSVEYASYSGGADLLADNHLDVFAFSVGKVASIVMQ
                     IESQTDIVLLGMEQSTLDKVGEAYGTVTFTVDPGIYKSVTETTPTVRVVGDYTCVVVR
                     GDLDEQLVYDLCKVMYENQETLSKAVVDINELTPATAIPGGAIKSHPGAVRYWNEVSK
                     K"
     gene            2263..>3866
                     /locus_tag="P5219_02190"
     CDS             2263..>3866
                     /locus_tag="P5219_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HRX25596.1"
                     /translation="MRKLDGIVAKGLYVYVLAMGLFHIYTAVFGTFEAYLQRAIHLTW
                     VLPMCFVLYPLAANGKTKVIETSVPWYDWIFAALSALPGIYIMLNYDEIMMRMQGVDD
                     LTTAQLVLGTLLILLLLEGTRRIVGLPIVIVAVFFTVYTYYCDAPFLPQLLRGVPTEF
                     QRLIEGMYLTDEGIFSSSLGVSATFVMIFLIFGGFLEKSGVGEYFMEFAQAFTGTAPG
                     GPAKIAVLSSALFGSISGSAVANVYGTGSFTIPLMKRIGYKPFFAGAVEAVASTGGQI
                     MPPVMGAGAFVMAALLGVPFNTIMIAAMLPALLYYGTVLLMVHLTALRDGLKGLPADE
                     LPTIRSVMKKLYMMSPIVLLVYMLLAGYTPMYGAIAGISLAWAVSLFNPGKRMGPKEI
                     LQAIHDGSENIPLICTACASAGLVIGSVALSGIGFKFVGAVLALSGGIPFLALVLIAV
                     VSLVLGMGLPTTSAYILGAALGVPALAKIGIMPLAAHLFVFYYAIISNITPPVALAAY
                     AAASIAGSPPNRTGFAACRLGILAFIV"
BASE COUNT          752 a         1230 c         1039 g          845 t
ORIGIN      
        1 ggtttccggc tcgtccgcga ggggcacgtt gagatcgtac atccgcagct cctcgagccc
       61 gagggctttt ttccgaaggg ccatgtagcc gtggagagcg tcgagattcc tgttcaccgt
      121 gtccaccacc atggcgtaga cggaagggga gatattgtcc ccatgaaggg acgcctcgag
      181 ggccgagggg tacttcctgg cccgggagaa gaagagatcg gatttcacgc tcccggagta
      241 cagggcggcg agagtgttcc ggaatttccc gtaggtggag aagagcccct cgaaggcgtc
      301 cttccgtacc cgcctgtctt tcgaccggct cagaaggtag taccgttctt ccgagagttc
      361 ggtctccctg cccgattcgt cgagaatctc cggaaactcg aggtcggcgt tcgtcagaag
      421 ggagaaggcg ttttccgggg ttcgggccat ctccccggac cgggcgagaa tctcctcctc
      481 ctgcaggccg agcacatgtc ccgccgtccg aagaaggtcg tcaaagtgat gactgtacat
      541 ttcgagcccg ggacagcctt cggcggcggc cttcaccccc tccgccccgg cggcgaggac
      601 ttcgggcctg tagaaggaga ggacggctcc ataggaggcc agcagggttc ccgccctgtc
      661 tgcccgtgcc tgccgggcag cgtcggcggt atcctcgtgg ctcttcatta ccgcataggc
      721 atagatcttg cccattcttt ccgaagcagc ttcctcgagc ttcaggaact ccagcagcgt
      781 ttccggggac tcgaaaagcc ttccgttaaa gccgccgagc cgctccagtt cttctccaag
      841 gacccggcag gattcctccc acgcttcgct tgaaggaaaa atggcatcaa ggtcccaggt
      901 gtactccccg ggaatttcgc ttctcggagg gattctgccg gagggatcgg ctcctccggc
      961 agaggatcgt gaaaacggac gacaatggat catgattgaa aactcctttc gttgtgaagg
     1021 ctcaaggaaa aaaaaggtcc gccggttcat tgacaccttt ttggagagcc cttattattt
     1081 ctttaacgaa ttcgaaggaa ttctaatcca tattaacagg agtgtgaatc gaaaaatgaa
     1141 aaagcgcgtt ctcgttcttg cagcccttgc gctcatcttc gccctgagcg gttcagccgt
     1201 ttccgcccag accgtaaagc ccgaccagct ccgcttcatg gcgggccctc cgggcggcaa
     1261 ctggttcgcc ctcggaggcg ccctggccga cctctggacc tccaagctga tccccaccac
     1321 cagcatcacc ggcggcgccg tggccaacat catcaatacc cacaacgcca agggcgagct
     1381 cggcttctcc aacacctcca tggtcgccgt gggccagaag gcgagcgacg cccccttcaa
     1441 ggaccccacc ggcaacactc tcatcatggc aaatctctac acccagtaca cctatttcat
     1501 cgcccggaag gatttcgtgg agaagcacgg gattacatct ctcgacgacc tcgtcacaaa
     1561 gaaaataccc accaggttcg ccaccctcaa gacgggcacc gggtccgagt tcatcgtcaa
     1621 cggcgtcttc aaggccgggt tcggcatcgc cgactacagg aaggaactca aggactgggg
     1681 cggctcagtt gaatacgctt cctactccgg cggcgctgac cttctcgctg acaaccacct
     1741 cgatgtcttc gccttctccg tgggcaaggt cgcttccatc gtaatgcaga tcgagagcca
     1801 gacggacatc gtcctcctcg gaatggaaca gtccaccctc gacaaggtcg gcgaagccta
     1861 cggcaccgtc accttcacgg tggacccggg aatctacaaa tccgtgactg agacaacccc
     1921 gacagtaagg gttgtgggcg actacacctg cgttgtggtc cgcggcgatc tcgacgagca
     1981 gctggtctac gacctgtgca aggtaatgta cgagaaccag gagactcttt ccaaggcagt
     2041 ggtggacata aacgaactca ctcccgcgac ggcgatcccc ggcggcgcca taaagagcca
     2101 ccccggagca gtccgctact ggaacgaagt ctccaagaag tagtatccca aactttcgct
     2161 tactgatccg gaaggcgggc tggttcagcc cgccttcgct ttcgttccgg acgttttgac
     2221 tgttacctgc agcccctgtg ttttcaagga ggaatacatt tcatgaggaa actcgacggc
     2281 atcgtggcaa agggtttgta cgtctatgta ctggccatgg gcctttttca catttatacc
     2341 gctgtgttcg gcaccttcga ggcctatttg cagagagcaa tccaccttac atgggtcctg
     2401 cccatgtgtt ttgtgctcta ccctcttgcc gcaaatggga agaccaaggt cattgaaact
     2461 tcagtaccat ggtatgactg gatttttgcc gctctttccg ccctgccggg catctacatc
     2521 atgctgaact acgacgagat catgatgcgg atgcagggcg tggacgacct gacgaccgcc
     2581 cagctcgtcc tgggaaccct gctgattctt ctcctgctcg agggaacgag gcgtatcgtc
     2641 ggccttccca tcgtcattgt cgcggtgttt tttaccgtat acacctatta ttgcgacgct
     2701 cccttcctgc cccagctcct caggggcgtt cccacggagt ttcagcggct catcgaggga
     2761 atgtacctca ccgacgaagg cattttttcg tcctccctgg gggtttcggc aacttttgtc
     2821 atgatctttc tgatcttcgg aggatttctt gagaaaagcg gcgtaggcga atacttcatg
     2881 gagttcgccc aggccttcac cggtacggct cccggcggac cggcgaaaat cgccgtgctc
     2941 agttcggccc tcttcggctc catttccggc tccgccgtgg ccaatgtcta cggtaccggg
     3001 tcctttacca ttcccctcat gaagcgtatc ggctacaaac ccttcttcgc cggcgcagtc
     3061 gaagcggtgg caagcacggg aggacagatc atgccccctg tcatgggagc gggagccttc
     3121 gtcatggcag cccttctcgg cgtacccttc aacaccatca tgatcgccgc catgctgcct
     3181 gcattgctct attacggcac ggtcctcctc atggtccacc tgacggcact ccgggacggt
     3241 ctcaaggggc ttcccgcaga cgagcttccc acgatcaggt cggtgatgaa gaaactctac
     3301 atgatgtctc ccatcgtcct cctggtatac atgctcctgg cagggtacac ccccatgtac
     3361 ggcgccattg cgggcatctc actcgcctgg gctgtttctc tgttcaaccc cggaaaaaga
     3421 atgggaccga aagaaattct ccaggccatc cacgacggat cggagaacat ccccctgatc
     3481 tgcaccgcct gcgcttcagc cggccttgtc atcggctcgg tggccctgtc gggcatcggc
     3541 ttcaaatttg tgggagccgt tctcgctctc tccggcggca ttcctttcct cgcccttgtc
     3601 ctcatcgccg tcgtctcact ggtcctgggc atgggacttc ccaccacgag cgcctacatc
     3661 ctcggagccg ccctcggtgt gccggccctg gccaagatag gcatcatgcc cctggctgcc
     3721 caccttttcg tcttctacta tgccatcatc tcgaacataa cgcctcccgt ggccctggca
     3781 gcctacgccg ccgcatccat cgccggctct ccgcccaaca ggacaggatt tgccgcatgc
     3841 aggctgggga tccttgcctt catcgt
//
LOCUS       DAOTHD010000078         3859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_27968_length_3859_cov_2.900368, whole genome shotgun sequence.
ACCESSION   DAOTHD010000078 DAOTHD010000000
VERSION     DAOTHD010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27968_length_3859_cov_2.900368"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="P5219_02195"
     CDS             <1..1104
                     /locus_tag="P5219_02195"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25597.1"
                     /translation="YREILDPVRVVAGGTNPFTPEGLMAMYEAALSPDLRVVTSNTTE
                     AGIFFEERRTPHNYPSLLAAALEKRAAEGLPPLHVVPLELIENNGAVLKECLEKYGKL
                     WNYGPAYFSYLEGCTFYDTLVDRIVTGFPAKEAAEVFRKIGHEDRNVTAGELFHLFVL
                     QGDKSILDVLPFHKAGLNAVITGDRLSFYRDRKVRVLNGVHTASVPVALLAGVEYVKD
                     FVEDPRFAPRLRSLVHDEIVPAFSDDAEAHQYGDDVLERFRNPALEHAFRSIALNSVA
                     KSNTRLRPTLEGYFGKFGELPPILTECIAAMTELYDCDGVKDLPGGPLELSDHRQLRG
                     RSLAEMTDSFFPGLSPDLRAVLLSRLGELRGVK"
     gene            complement(1177..2478)
                     /locus_tag="P5219_02200"
     CDS             complement(1177..2478)
                     /locus_tag="P5219_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRX25598.1"
                     /translation="MPDPSTASWLLLGSFAVFLVLKVPITFSLAVSSIITAVYLKIPL
                     MAIMQQMVQGVNSFSLLAIPFFIVAGEMMGEGGISRRLIAFSNLLVGRVRGGLAQVNV
                     LASMFFGGISGSAVADVSSIGTILIPMMKKEGYDADYSVAVTVTSACQGIIIPPSHNM
                     IIYSLAAGGVSVGRLFLGGFVPGVILGIALMIISYIIAVRRNYPKEQPCTWKEALRIS
                     REAVLGIFTAVIIIGGVISGVFTATESAAVACVYAFFVTFFVYREIPLSRMNKILYSS
                     LKTLAMVMSLIAAAKAFGWLLSYLKIPALATSALLAITDSPVLLLLLVNLLLLGLGCI
                     MDMAPLILITTPILFPVVVGTLGMHPVQFGIMMMLNLGIGLCTPPVGSALFVGCAVGK
                     ISLEKATRAMLPFYAAMVVVLLIITFVPQVVLFVPELVMGK"
     gene            complement(2471..2998)
                     /locus_tag="P5219_02205"
     CDS             complement(2471..2998)
                     /locus_tag="P5219_02205"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRX25599.1"
                     /translation="MSLKFFTAVFRFIHLALVAFAKILLLAMVVLIFSNVFMRYVLNS
                     GIPWSEEVSIVIVVWFTFISLALGVKHRLHISLCLLPERISPRTDFLLAKITDLATLF
                     LGYVMVRYGWILVQFTSRSILPATEFPASVMYFPLVLSGILVTYEGFMNLLGLDKGDA
                     GMDEKLSGRRCVSDA"
     gene            complement(3057..>3859)
                     /locus_tag="P5219_02210"
     CDS             complement(3057..>3859)
                     /locus_tag="P5219_02210"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRX25600.1"
                     /translation="IEVYHSKQLGEEKAVIEQVQFGAIDFTRVSISPLAAFAPAFDAL
                     QMPYLYRGEEHMWKVLNGPIGEEFLNSLEPANFVGLAWYDSGARNFYNSKREIKSVAD
                     LKGLKIRVQESKLMMGLVSALGAVPTPMPFGEVYSALQTGVIEGAENNWPSYFSTSHY
                     EVAKYFTLDGHTRVPEILIASKMTMDRLSKEDQDVIRKAAKDSMPFQIKLWKEFEKVS
                     EEKVTAAGSIVTHLTPEAVAEFQKAMEPLYSELTPELQAMVKKIQEVK"
BASE COUNT          820 a         1078 c         1230 g          731 t
ORIGIN      
        1 taccgtgaaa tactcgaccc cgtccgggtg gtggccggag gaacgaaccc cttcaccccg
       61 gaaggtctga tggccatgta tgaagcggcc ctctccccgg acctcagggt ggtgacgtcc
      121 aacaccaccg aggccgggat cttcttcgag gagagaagga ctccccacaa ttatccttcc
      181 ctcctcgccg cggccctcga gaaaagggcc gcggaaggat tgccccccct tcacgtcgtt
      241 cccctggagc tgatagagaa caacggcgcc gtcctgaagg agtgcctgga gaaatacggg
      301 aagctctgga actacggtcc ggcctatttc agctacctcg aggggtgtac cttctacgac
      361 accctggtgg accgcatcgt caccggattc cccgcaaaag aggcggcgga ggtcttccgg
      421 aagatcgggc acgaggacag aaacgtcacc gcaggggaac tcttccacct ctttgtcctc
      481 cagggagaca aatccatcct ggacgtgctt cccttccaca aggccggttt gaacgccgtg
      541 atcaccggtg acaggctgtc gttctaccgg gacagaaaag ttcgggtgct caacggcgtc
      601 cacacggcct ccgtcccggt ggccctcctt gccggcgtgg aatacgtgaa ggatttcgtg
      661 gaggatccgc ggttcgctcc caggctgagg agcctggtcc atgacgagat tgtccccgcc
      721 ttctccgatg acgccgaagc acaccagtac ggcgacgatg tcctggaacg gttcaggaat
      781 cccgccctgg agcacgcctt ccggtccatt gccctgaatt cggtggccaa gtcgaacaca
      841 aggctccgtc ccaccctgga ggggtatttc gggaaattcg gcgagctgcc ccccatcctc
      901 acggagtgca ttgcggcgat gacggagctc tatgactgtg acggcgtgaa agatctgccc
      961 ggcggacccc ttgagctttc cgaccaccgg cagcttcggg gcaggagcct cgcggagatg
     1021 accgacagct tcttccccgg cctgtcgccg gatctcaggg cggttcttct ttcccggctc
     1081 ggagagcttc ggggagtaaa ataggatttt tcccggattc cggaaaaaca gcccggccct
     1141 tccgatcgga agggccgggc tgtttttttc tttttcctat ttccccatga ccagttcggg
     1201 aacgaagaga accacctggg gcacgaaggt aatgatcagg agcaccacca ccatggccgc
     1261 atagaaaggc agcatggccc gggtcgcctt ctccagggaa atcttcccca ccgcgcaccc
     1321 gacaaagagg gcggagccca ccggaggcgt gcagaggcct atgcccaggt tgagcatcat
     1381 catgatgccg aactggacgg ggtgcattcc cagggtgccc accaccacgg ggaagagaat
     1441 gggagtggtg atgaggatca ggggggccat gtccatgatg cagcccagtc caaggagaag
     1501 aaggttgacg agaagcagca ggagcaccgg gctgtccgtt attgcaagca gcgcgctcgt
     1561 ggcgagggcc gggatcttga ggtacgaaag cagccacccg aaggccttcg ccgcggcgat
     1621 gaggctcatc accatggcga gggtcttgag ggaggagtag agaatcttgt tcatccgtga
     1681 caggggaatt tccctgtaga cgaagaaggt gacgaagaag gcataaacgc aggcgacggc
     1741 cgccgattct gttgccgtga agacgccgga gatgacgccg ccgatgatga tgacggcggt
     1801 gaagattccg aggacggcct ccctggaaat ccggagggcc tccttccagg tgcagggctg
     1861 ttccttgggg taattccgtc tcacggcgat gatgtagctg atgatcatga gggcgatgcc
     1921 gaggatgacc cccgggacga agccgccgag aaagaggcgt cccaccgaga cccctcccgc
     1981 ggccagggag tagatgatca tgttgtggct cggggggatg atgatgccct ggcatgcgct
     2041 ggtcaccgtg accgccacgg agtagtcggc gtcgtatccc tcttttttca tcatggggat
     2101 gaggatggtc ccgatggagg acacatccgc cacggcggag ccggaaatgc cgccgaaaaa
     2161 catgcttgcc aggacgttca cctgggcgag gcctccccgg accctcccca ccaggaggtt
     2221 ggagaaggcg atgagccgcc tggaaatgcc tccttcgccc atcatttcac cggcaacgat
     2281 gaaaaacggg atggcgagga gggagaagga gttcactccc tggaccatct gctgcatgat
     2341 ggccatgagg gggatcttca ggtagacggc ggtgatgatt gaggataccg ccagggagaa
     2401 tgtgatggga accttgagga ccaggaagac ggcgaaggat ccgaggagaa gccaggaggc
     2461 ggtggaaggg tcaggcatcg gaaacgcacc tcctcccgga gagtttttcg tccatgccgg
     2521 cgtctccctt gtcgaggccg agaaggttca tgaatccctc gtaggtcaca agaattccgg
     2581 agagaaccag cggaaagtac atcaccgacg cgggaaactc ggtggcggga aggatggatc
     2641 tgctggtgaa ctgaacgaga atccacccgt acctgaccat gacgtatccc agaaaaaggg
     2701 tcgccaggtc cgtgattttc gccaggagga agtccgttcg gggagaaatc cgttcgggca
     2761 gaaggcagag gctgatatga agacggtgtt tcactcccag ggcaagggag atgaaggtga
     2821 accagaccac gatgacgatg gaaacttcct cggaccacgg gatgcccgag ttcaggacgt
     2881 atctcatgaa gacgtttgag aaaataagga ccaccatggc caggaggagt attttggcga
     2941 aggccacgag ggcgagatga atgaatctga agacggcggt gaaaaatttc aagctcattc
     3001 ccccccggaa aggaaaaaaa ggggaaagcc tgtacaggct tccccctgcg ggacagctat
     3061 ttcacttcct ggatcttctt gaccattgcc tgaagttccg gcgtgagttc ggagtagagg
     3121 ggctccatgg ccttctggaa ttcggcgacg gcttccggag tgaggtgggt gactatcgac
     3181 ccggcagcgg tgactttttc ctcggagacc ttctcgaact ctttccagag cttgatctgg
     3241 aaaggcatgg aatcctttgc cgccttgcgg atgacgtcct ggtcttcctt cgagagcctg
     3301 tccatggtca tcttgctggc gatgaggatt tcggggaccc gggtgtggcc gtcgagggtg
     3361 aagtacttcg ccacctcgta gtggctcgtg gagaaatagc tcggccagtt gttctccgct
     3421 ccttcgatga cgcctgtctg gagggcgctg tagacctcgc cgaagggcat gggggtggga
     3481 acggcgccga gggcggagac aaggcccatc atgagcttgc tctcctggac ccggatcttc
     3541 agtcccttca ggtcggccac ggacttgatc tccctcttgg agttgtagaa attccgggcg
     3601 ccggaatcat accaggccag gcccacgaag ttggcgggct cgaggctgtt caggaattct
     3661 tcgccgatgg gtccgttgag gaccttccac atgtgctcct ctccccggta aaggtagggc
     3721 atctgcaggg cgtcgaaggc gggggcgaag gctgcgagag gagagatgct cacccgggtg
     3781 aagtcgatgg ccccgaactg cacctgttcg atgacggcct tttcctcgcc gagctgcttg
     3841 ctgtggtaga cctcgatct
//
LOCUS       DAOTHD010000079         3857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_27987_length_3857_cov_3.062862, whole genome shotgun sequence.
ACCESSION   DAOTHD010000079 DAOTHD010000000
VERSION     DAOTHD010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27987_length_3857_cov_3.062862"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..910)
                     /locus_tag="P5219_02215"
     CDS             complement(77..910)
                     /locus_tag="P5219_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRX25601.1"
                     /translation="MTMLRITTLIENSPGEHHGLATEHGISFCIEKDGHIVIFDTGQS
                     GAFLDNAPKLNINLSGAGHVILSHGHYDHSGGFRALCSAAGGFTLITGEGFFVPKYAF
                     RNGAYDFLGNDFDEAFLSERGITSVTASAPAKEILPGIHVLSGFPRIHTEEKINPRFV
                     LEKDGAFVPDSFDDEILLAVETGEGLAVLLGCSHPGMRNMLDAVKERLGRPILAVLGG
                     THLVEAKGTGLEKSLEYLQNSGILFVGMSHCTGKDVMDRLEAVCPGFFRNVTGSSLFF
                     G"
     gene            complement(907..1626)
                     /locus_tag="P5219_02220"
     CDS             complement(907..1626)
                     /locus_tag="P5219_02220"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HRX25602.1"
                     /translation="MTPIRKTRLSEQVVDAIETMIAEGEFSPGDRFFSEAELGRRLEV
                     SRSSIREAVRILEVRGLVSVRHGKGIFIAEPCGEEEDPFRGWLKDNKTSLLDHFEVRL
                     IIEPRAAWHAAAKADAESVRAMEKACGAFAANAESGDTAALVKADGEFHRLIARSTKN
                     RTLYCLMKTMTSSLSEGWITSLHIPGRIEKTMGEHYAVFEAIRDGDPERAEKEMVRHL
                     TNALGDIRDSMERPAGRPEEE"
     gene            complement(1774..2481)
                     /locus_tag="P5219_02225"
     CDS             complement(1774..2481)
                     /locus_tag="P5219_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRX25603.1"
                     /translation="MKERILVAEDDRAILTGVADLLESEGYEVIPAGNGAEALELFRR
                     NSPDLVLLDVMMPKMSGYDVCRAIRGENAVVPVVMLTAKGQEVDRVVGLELGADDYIV
                     KPFGMAELLARVRAALRRGALAAGTQGAGEESRLAFGNVQVDFGAMTGSRGDVSFSLT
                     PRENALLRFFAGREGKVLTRDLLLEEVWGIDCDVTTRTVDQHVVRLRQKIEDDPSKPV
                     YLLTAHGAGYRFSTGKE"
     gene            complement(2478..>3857)
                     /locus_tag="P5219_02230"
     CDS             complement(2478..>3857)
                     /locus_tag="P5219_02230"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRX25604.1"
                     /translation="PLPAPAKKAAAPQGGIERQMAQSMAAADPAFREELFRKAEEEGF
                     SLLQRNVAPQAGAAPSPEREERSGTVARGKSFAALRSESEGGFLPRLSERGLELIFWT
                     DVPGGAAGFSIDMDDLRDRIAGAAPEALTDIRVLTILDDRGNPLVSPPLPSGADWRRP
                     FVAREISPLLPRWEAGAWLTNPGLAASRARFATIAVWLLTGILFVVILAGAGVIMRML
                     SAEMRLAANKTTFVANVSHELKTPLTSIRLFAEMLLSGRQQSEERRKEYLRTMVSETE
                     RLSRLVDNVLAFSRKGADIRPLPMERLDLAALAEDTLAQLEPHLAKNGFVSGFSAEGP
                     LPVLGNSEALRQVLMNLLSNSEKYSPAVREVTVCCGREGETAAVRVADRGSGVPPGLG
                     EKIFQEFFRGDDSLAAPRSGAGLGLAIARDIARRHGGDVKYDPREGGGSVFSLVLPLA
                     FPEGKERRG"
BASE COUNT          708 a         1235 c         1154 g          760 t
ORIGIN      
        1 taacctcctt tgtatttggt gtttccctac ggccggggag gaatttcccc ggccctgaac
       61 gacacccttt cagaagctac ccgaaaaaga gggaactgcc cgtcacgttc cggaaaaagc
      121 ccgggcagac cgcctcgagg cgatccatga catccttccc cgtacagtgg gacatgccga
      181 cgaaaagaat gccactattc tgaaggtatt cgagagattt ttcaagaccg gtccctttcg
      241 cctccacgag atgggtcccg ccgagaacgg cgaggatagg ccttccgagc cgttctttca
      301 cggcgtcgag catgtttcgc attcccgggt gggagcatcc caggagcacg gcgagcccct
      361 ctcccgtctc gactgccagg agaatttcat catcaaagga atcgggcacg aaggcgccgt
      421 ctttttccag gacaaaccgc ggattgattt tttcctccgt gtggatccgg ggaaaacctg
      481 aaagaacgtg gattccggga agaatctcct ttgcgggggc ggacgcggtt acggaagtga
      541 ttcccctttc ggagagaaag gcttcgtcga agtcgtttcc aagaaaatcg tatgcaccgt
      601 tccggaaggc atacttgggg acgaagaatc cttcgccggt tatgagggtg aagcccccgg
      661 cggcggagca gagggcgcgg aatccgccgg agtggtcgta gtgcccgtgg ctcaatatca
      721 cgtggcctgc accggaaagg ttgatgttca gtttcggggc gttgtcaaga aaggctcccg
      781 actgccccgt gtcgaagatg acaatgtgtc cgtccttctc gatgcagaag gagattccgt
      841 gttccgtcgc cagtccgtga tgttcaccgg gagaattttc gatgagggtg gtgatccgca
      901 gcatggtcat tcctcctccg ggcggccggc aggccgctcc atggaatccc tgatgtcgcc
      961 gagggcattg gtcaggtggc gcaccatctc tttttccgcc cgttccggat ctccgtcccg
     1021 aatggcttca aacactgcgt aatgttctcc catggtcttc tctatccggc cgggaatgtg
     1081 gaggctggtg atccagcctt cggacaggga tgaggtcatg gttttcataa ggcagtagag
     1141 ggtcctgttt ttcgtggatc tggcgatgag gcggtgaaat tcgccgtcgg ccttgacaag
     1201 ggcggcggtg tcccctgatt ccgcgttcgc ggcaaaggca ccgcaggcct tttccatggc
     1261 ccggacggat tcggcgtccg ctttcgcggc ggcgtgccat gcggccctgg gttcgatgat
     1321 gagccgaacc tcgaagtgat caagcaggga ggttttgttg tccttcagcc acccccggaa
     1381 ggggtcttcc tcctcaccgc atggttcggc gatgaaaatg cccttcccgt gccggacgga
     1441 gaccagcccc ctcacttcga ggatcctgac cgcctcccgg atggaggacc ggctgacctc
     1501 gagcctcctg cccagttccg cctcggagaa gaacctgtcc ccgggactga actccccctc
     1561 ggcgatcatg gtttctattg cgtccaccac ctgctccgac agcctggttt tccgtatcgg
     1621 cgtcatggtc cctcccgccg ccggaggcgg catacagcat atctactcat cagacaacct
     1681 gaattctact ccctttttcc tgaaagtgtc aagaaaagaa aaaaaaggcc ccgaagggcc
     1741 gggtggtacg gtccggaaac ggtttccccg ggtttattcc tttcctgtgc tgaaccggta
     1801 gcccgcaccg tgagccgtga gaaggtacac gggtttcgat gggtcgtcct cgatcttctg
     1861 gcggaggcgc accacgtgct ggtccaccgt ccgggtggtc acgtcgcagt cgatgcccca
     1921 gacctcttca aggagaagat ccctggtgag gacttttcct tcccggccgg cgaaaaaacg
     1981 gagaagggcg ttctccctgg gagtgaggga aaaggaaaca tctcccctgc ttcccgtcat
     2041 ggcgccgaaa tcgacttgca cgttcccgaa ggcaaggcgg ctttcttccc cggccccctg
     2101 tgtcccggcc gcaagggctc cccttcgaag ggcggccctc accctggcga gcagctccgc
     2161 catgccgaag ggcttcacga tgtagtcgtc cgcccccagt tcgaggccca caaccctgtc
     2221 cacctcctgc cccttggcgg tcagcatgac caccgggaca acggcgttct ctccccggat
     2281 ggccctgcag acgtcatagc cgctcatctt cggcatcatg acgtcaagaa gcaccaggtc
     2341 gggagagttc cggcggaaca gctccagggc ctcggcgccg tttcccgccg ggatgacttc
     2401 gtacccctcg ctttcgagaa ggtccgccac cccggtgaga atggcccggt cgtcctcggc
     2461 tacaagaatc cgctccttca tcctcttctc tcctttcctt ccggaaacgc aaggggaagc
     2521 accagggaga aaacgctccc tcccccttcc ctcgggtcgt acttcacatc cccgccgtgc
     2581 cttctcgcga tgtcccgggc gatggcaagc ccgagcccgg ccccgctcct cggcgccgcg
     2641 agggaatcat ccccccggaa gaactcctgg aaaatctttt ccccgagccc ggggggcact
     2701 ccggagcccc tgtcggccac cctcacagcg gccgtttccc cttcccttcc gcagcagacg
     2761 gtgacttccc ggactgcggg ggaatacttc tcgctgtttg acaaaaggtt catcaagacc
     2821 tgccggaggg cctcgctgtt tccgaggacc ggcaggggac cttcagcgga aaagcctgac
     2881 acaaatccgt ttttcgccag gtggggctca agctgcgcca gggtatcctc cgccagggcc
     2941 gcgagatcca gccgttccat gggaagggga cggatgtccg ctcctttcct cgaaaaggca
     3001 aggacgttgt caacgagccg ggagagccgt tccgtctctg acaccatggt cctgagatac
     3061 tccttcctgc gctcctcgct ctgctgcctt ccggagagaa gcatttcagc gaacagccgg
     3121 atggaggtca gcggggtctt cagctcgtgg gagacgttcg ccacaaaggt cgtcttgttc
     3181 gccgcgaggc gcatctcggc ggagagcatc ctcatgatca cccccgcgcc ggcgaggatg
     3241 accacgaaca ggattccggt gagcagccag acggcgatgg tggcgaatct cgccctggag
     3301 gctgcgaggc ccggattggt aagccacgcc cctgcctccc agcggggaag gaggggggaa
     3361 atttccctgg cgacgaaggg acggcgccag tcggctcctg acggaagagg gggagagaca
     3421 agagggttcc cccggtcatc caggatggtg agcacccgga tatccgtcag ggcttcggga
     3481 gccgccccgg caatacggtc ccgcagatcg tccatgtcga tggagaagcc cgctgccccg
     3541 ccggggacat ccgtccagaa aatcagctcc agtccccttt ccgagagcct tgggagaaaa
     3601 cctccctccg actcggaacg cagggcggcg aagctcttcc cccgggccac cgtgccggac
     3661 cgttcctccc gctcgggaga aggggcagcc cccgcctggg gggccacgtt ccgctgcagg
     3721 agactgaaac cttcctcctc tgccttccgg aagagttctt cgcgaaaagc ggggtcggca
     3781 gcggccatgc tctgggccat ctgccgctca atccccccct ggggtgccgc cgccttcttc
     3841 gcaggggcgg gaagggg
//
LOCUS       DAOTHD010000080         3838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_28156_length_3838_cov_3.000529, whole genome shotgun sequence.
ACCESSION   DAOTHD010000080 DAOTHD010000000
VERSION     DAOTHD010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28156_length_3838_cov_3.000529"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="P5219_02235"
     CDS             complement(<1..214)
                     /locus_tag="P5219_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRX25605.1"
                     /translation="MKKSDIGLIGLAVMGENLVMNMESRGYTVSVFNRTADKVSNFIE
                     GRAKGKNILGTFFLEDLVASLECPRKI"
     gene            complement(265..1347)
                     /locus_tag="P5219_02240"
     CDS             complement(265..1347)
                     /locus_tag="P5219_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HRX25606.1"
                     /translation="MMKPTVRENCAYSLVSPTSMGVRITPLNHQPVHVSDHFFLQATS
                     AESNVLTVSAALGERVKVLTTFVKDSPIALLIKNDLRKRGIEFEGKDVPQGGPWGYRH
                     QFNIADTGYGVRGPRVTNDRAGEVGRTLNVKDFDLERLFGREGVRVLHVSGLIAALSP
                     ETGQFCIELARAAKSHGTLISFDLNYRESFWKGRGEELHSLFSEIASLTDYLIGNEED
                     FQLALGVQGPEAGGADIAGKMDSFKGMIDNARKAFPNASVFATTLRQVVHANEHLWGA
                     ILHSDEGWFQAPPRPIHILDRIGGGDGFVGGLLYGLNKGWEPEKALQFGWATGAMATT
                     MIYDFAVPASEEQVWSVWEGNARVKR"
     gene            complement(1585..>3838)
                     /locus_tag="P5219_02245"
     CDS             complement(1585..>3838)
                     /locus_tag="P5219_02245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX25607.1"
                     /translation="LKFVAFEAEKQLSLHFAVRKDWPELVSVLDKTLASITQEERLAI
                     NAKWIDLGTETDYGPLIRNLLIAGSAVFLILLVSLCWVWILKREAARRVKIQEDLEKA
                     KLEAEAANNVKSGFMARMSHEVRTPLNAINGMAYLLKKSELAPTQKMYVDRIIQSSNT
                     MLSIINDILDFSKIEAGKVELEHIAFNIDNVLQNVIDIVSYKIEEQRIGFSLTKDPRL
                     PNFFFGDSRRIEQILINLINNAAKFTESGTISFDVRMKARKASTCLLLFTIEDTGIGM
                     TEEQANRLFEPFSQGDASINRRFGGTGLGLAIVKNLLDMMGGEIQVYSSEGRGTTFII
                     QVELEVDAANEEEHRKRISSSYFRNIKTLVLEKTGANMNLIDSYLSSFGMECEITTSE
                     ASARSMLETANDKFSKPFDLLIIDYETPEEKAFSFVDKMRVNPRIVKMPAVILLLPMM
                     REDLFDRTEEHGIEMAVGKPVIQSVLFNAIQEIFKLKAVSSVGGESQDKKEQVAEEEM
                     KTSLGTILVVEDNKTNQLIAKSLLEQEGFSIILADDGEKGVAAFREHFGEIRLVLMDL
                     HMPVLNGYDASLRIKETAPAVPIIALTADVVDGVREKCEQHGITDYISKPFDPEGFVR
                     KVVETVGARPLNVPKEKGILDRAMGLRFMGNNEALYKTVLSEYARENGTLVRDLAALV
                     EAGQYDDAAKMVHKVKSSSGSIGATALHETARDLQRALEDSDGEKIRELHGTFSRQME
                     TLLAEIGTDL"
BASE COUNT          672 a         1125 c         1135 g          906 t
ORIGIN      
        1 tgatcttccg ggggcattcg aggctcgcta ccaggtcctc gaggaaaaag gtgccgagaa
       61 tgttcttccc cttcgccctg ccttcgataa aattggaaac cttgtccgcg gtacggttga
      121 acacggagac ggtgtatccc ctggactcca tgttcatgac gaggttttct cccatgaccg
      181 ccaggccgat gaggccgata tcagactttt tcatggcaca gctccttttc ccttccaatt
      241 tccgccccac gctcgaagcg gtccctaccg ttttacccgg gcgttgcctt cccagacgct
      301 ccagacctgc tcttcgctcg cgggaacggc gaagtcgtag atcatggtgg tggccatggc
      361 gccggtggcc cagccgaact gaagtgcctt ctcgggctcc catcccttgt tcaggccgta
      421 gagcagaccg ccgacgaacc cgtcgccgcc gccgatgcgg tcgagaatgt ggatgggccg
      481 gggcggagcc tggaaccagc cctcgtctga atggaggatg gcgccccaga ggtgttcgtt
      541 ggcgtgaacc acctggcgca gggtggtggc gaagaccgag gcgttgggga aggctttccg
      601 ggcgttgtct atcatgccct tgaagctgtc catcttgccg gcgatgtccg cgccgcctgc
      661 ctccggtccc tggacgccga gggcgagctg gaaatcctcc tcgttgccga tgaggtagtc
      721 cgtcagggag gcgatttcgc tgaagagaga atgaagctcc tctcccctgc ccttccagaa
      781 ggattcccgg tagttcaggt cgaagctgat gagggtgccg tggctcttgg cggcccgggc
      841 cagctcgatg cagaactgtc ccgtctcggg ggagagggcg gcgatgaggc ccgagacatg
      901 gagcacccgt actccctccc ggccgaaaag ccgttcaagg tcgaaatctt tcacgttgag
      961 ggtccggccg acttccccgg cccggtcgtt ggtcaccctg ggcccccgga cgccgtagcc
     1021 cgtgtcggcg atgttgaact ggtggcggta cccccacggg cctccctggg gaacgtcctt
     1081 tccctcgaac tcgatgcccc gcttgcggag gtcgttcttg atgagaagcg ctatggggct
     1141 gtccttcacg aaggtggtaa ggaccttgac cctctctcca agggcggcgc tgacggtgag
     1201 gacgttgctt tcggcgctgg tggcctggag gaagaaatgg tcgctcacgt gcaccggctg
     1261 gtggttgaga ggcgtgatcc gcacccccat gctggtgggg ctcacaagcg aataagcgca
     1321 gttttcccta acggtcggtt tcatcatgtc ctccttctcg gcaggaatca gcctgcgaca
     1381 gaactgacta aaaacattgt ccgaagcggc ggaggcgggc tgtggctctc tcccgcccgg
     1441 ggacaaacct gcagtctccg cacccttcac cggatcgtcc gcactttttc acaatatagc
     1501 agaaaaccgg gcgaatggct ccccccgaaa acagaaaaag ggactcccct atcaggagtc
     1561 cccgtttctc attctcttgg tttttcagag gtccgtccct atttccgcca gcaaggtctc
     1621 catctggcgg ctgaaggtgc cgtgaagttc ccggatcttt tctccgtcgg aatcctcgag
     1681 agcccgctgg agatcccttg cggtttcgtg aagggccgtc gcaccgatgc tgcctgaact
     1741 gcttttcacc ttgtggacca tctttgcggc gtcatcgtac tgccccgctt ccaccagggc
     1801 ggcgagatcc ctgacaaggg tgccgttttc ccgggcgtat tcggacagca cagttttgta
     1861 gagggcttcg ttgtttccca tgaaccggag ccccatggcc ctgtcgagga tgcctttttc
     1921 cttcggtacg ttcagcggcc gggccccaac cgtttcgaca accttccgga caaagccttc
     1981 cgggtcgaag ggcttgctga tgtagtcggt tatgccgtgc tgttcgcact tctccctgac
     2041 cccgtccacc acgtctgccg tgagggcgat gatgggaact gcgggggcgg tttccttgat
     2101 cctgagtgac gcgtcgtacc cgttgaggac gggcatgtga aggtccatga gaacaaggcg
     2161 tatctccccg aagtgttccc ggaaggccgc cacgcccttt tcgccatcat ccgcgaggat
     2221 aattgagaac ccttcctgtt caagcaggga tttcgcgatg agctggtttg ttttgttgtc
     2281 ctccaccacg aggatggttc cgagagatgt tttcatttct tcctctgcca cctgttcttt
     2341 cttgtcctgg gattcgcctc cgaccgagga gacggccttc agcttgaata tttcctgtat
     2401 ggcattgaag aggactgatt gtatgaccgg cttgcccacg gccatttcaa ttccgtgctc
     2461 ttccgtcctg tcgaagaggt cttcccgcat catggggagc agaaggatga ccgccggcat
     2521 tttgacgatc ctcggattga ccctcatttt gtccacgaag ctgaaggcct tttcctccgg
     2581 tgtctcgtag tcgatgatga gaaggtcgaa gggcttggag aacttgtcgt tcgccgtttc
     2641 gagcatgctc cgggcgctgg cctccgatgt ggtgatctcg cactccatgc cgaaggagct
     2701 gagataggaa tcgatcaggt tcatgttggc tccggtcttc tccaggacga gggtcttgat
     2761 gttcctgaag taggaagagg atatcctttt ccggtgctct tcctcgtttg cggcgtcgac
     2821 ttccagctcc acctggatga tgaaggttgt tcccctgcct tccgaactgt agacctggat
     2881 ctcgccgccc atcatgtcca gcaggttctt cacgatggcc agcccgaggc cggtgccgcc
     2941 gaaacgcctg ttgatgctgg cgtccccctg ggagaaaggc tcgaagagcc tgttcgcctg
     3001 ctcctccgtc atgccgatgc ccgtgtcctc gatggtgaag agcagaaggc aggttgaggc
     3061 cttccgggcc ttcatccgga catcgaagga gatggttccc gattcggtga acttggcggc
     3121 gttgttgatg aggttaatga ggatctgctc gatgcgcctt gagtcaccga aaaagaagtt
     3181 cggcagcctg gggtccttgg tgaggctgaa gccgatcctc tgctcctcta tcttgtagga
     3241 gacgatgtcg atgacgttct ggagcacgtt gtcgatgttg aaggcaatat gttccagctc
     3301 caccttcccg gcctcgatct tcgagaagtc gaggatgtcg ttgatgatgc tcagcatggt
     3361 gttcgacgac tgaatgatcc ggtccacgta catcttctgg gtcggcgcca gttcgctctt
     3421 tttcaggaga taggccatgc cgttgatggc gttcagcggt gtcctcactt cgtgggacat
     3481 gcgggccatg aagcccgatt tcacgttgtt cgccgcttcg gcctccagct tggccttctc
     3541 caggtcttcc tggattttca ccctgcgggc cgcctcccgt ttcaatatcc aaacccagca
     3601 gagagacacg agcagaatga ggaagacagc agagcctgca atgagcaaat tcctgatgag
     3661 agggccgtag tccgtttcag tccccaggtc gatccatttc gcgttgatgg ccaaccgttc
     3721 ttcctgggtt atggaggcca gggtcttgtc caggacggag accagctcgg gccagtcctt
     3781 tcttacggcg aaatggaggg agagctgctt ttccgcctcg aaggccacga acttgaga
//
LOCUS       DAOTHD010000081         3816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_28377_length_3816_cov_3.539218, whole genome shotgun sequence.
ACCESSION   DAOTHD010000081 DAOTHD010000000
VERSION     DAOTHD010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28377_length_3816_cov_3.539218"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="P5219_02250"
     CDS             complement(<1..801)
                     /locus_tag="P5219_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRX25608.1"
                     /translation="MIYDAIIVGSGPAGVFAAMELVRHGKKVLIVDKGRVIRERKCPI
                     VEGKSKTCLNCESCSIVSGWGGAGAASDGKLTLTTGFGGNLEENIGEEALLDMISYVD
                     KTFVEYGADNNSYEPSGAVVKDTIRKASSVGLKILPARIRHIGTDASREVLNNMYEDL
                     VQKCDILMNTFVEDILVENGRAVGILLADGTERRGEYVIAAPGREGASWLAGLVEKLN
                     IPIASMPVDIGVRVEVPDSVCEDLTQHFYEVKCLYNTPTFDDRCRTFCM"
     gene            complement(870..1223)
                     /locus_tag="P5219_02255"
     CDS             complement(870..1223)
                     /locus_tag="P5219_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25609.1"
                     /translation="MALKICEHCTKAYLDGGEQEKTPLCPECAEYLEELYRQAWTWLR
                     DHSSADGRLRKVTSARLAEELGVDVRSIEVLVKMDRIQTSASSAKKVSGKDLETLKAL
                     KRGLSGSGRQRRERR"
     gene            complement(1233..1901)
                     /locus_tag="P5219_02260"
     CDS             complement(1233..1901)
                     /locus_tag="P5219_02260"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRX25610.1"
                     /translation="MGRVQVIVEGMTASGKSTVVNLLSDRLGLQVMPEEFRDQYDLLR
                     RFHHERRWAFPMQLNFLVTRFAQYLCASESENYILDRSVFGDKIYASLYYRLGYFRDS
                     QFGTYLMLYDSLLKHLAMPRLMIVLSCPFEEIMRRIRARGREDELAAGEPYWKSLYDA
                     YSSFREFVRSETGLSNGGLMTLDLSDPAFIHTPSRVESFVAEVKERLGPEGPPPGKKR
                     NAEE"
     gene            complement(1912..3681)
                     /locus_tag="P5219_02265"
     CDS             complement(1912..3681)
                     /locus_tag="P5219_02265"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRX25611.1"
                     /translation="MSESFDKPLGNEGTDSVNFMIQKTIRLEKEAARWKREETALRDM
                     ERRYLALSENPVFLLAILSGGRVTYLNGRAEAFFGFPLRERPRFLLTDYVAPGYCVEV
                     EKLLEQAGGDELWERRFSFPVLTEGGAERWLDMAVTPVEYRGEPSMLAVGYEIPAPAE
                     NEPVAETKIPAGFSCSADNLLAAVTDRDCILQFMTEGFRREAAPMWNAPPETGLSMLE
                     LLPEGDRGLPFRLAVEKALSGEGAEIGQEAGDRFFSISFAPVFSPDGQVSGVSLVLCE
                     KTGQRAAERKVRAEEEKFRRLFSLVSDLGIIVTRDEGRIIECNRAFHEKVFPSGERPE
                     GKTLEELGLLPYGALKNTLYSGIGEKGSVRELKQSITLPSGEDLPVLLSAIPLERGGR
                     QVLLLSLRETVVPAVQAPSAEAAPDPSGEKAPQERSRGIDELLGIPDREGFGRLLSTE
                     MERAGRYRGSLSVILMDIDGFRELMEQAGEDGGKKFLRDFFTAVKSRIRPTDFLARFG
                     GGEFAVLTPMSGYLAHQMADKIRDMVCHSHFFPGRGVFCSFGVCEYRREMSNDEFLKR
                     AGIALREAKRAGGNRAVLAPPIP"
BASE COUNT          735 a         1214 c         1064 g          803 t
ORIGIN      
        1 catgcagaag gtgcggcacc ggtcgtcgaa ggtgggggtg ttgtagaggc atttcacctc
       61 gtaaaaatgc tgggtcaggt cctcgcagac gctgtcgggc acttccaccc tgacaccgat
      121 atccacgggc atggaggcta tggggatgtt gagcttttcc accagtccgg cgagccagga
      181 cgcgccttcc ctgcccgggg ccgcgatgac atactctccc cggcgctcgg tcccgtcggc
      241 gaggaggatg cccacggccc gcccgttctc gacgaggata tcctccacga aggtgttcat
      301 gaggatgtcg cacttctgca ccaggtcctc gtacatgttg ttgagcactt cccgggaggc
      361 gtcggtgcct atatggcgga tgcgggcggg aaggatcttg agcccaacgc tggaggcctt
      421 tcgtatggtg tccttcacca cggcccccga cggctcgtag gagttgttgt cggcgccgta
      481 ttcgacaaaa gtcttgtcca cgtaggagat catgtcgaga agagcctcct cgccgatgtt
      541 ttcctcgagg ttgccgccga atcccgtggt cagggtcagc tttccgtccg acgcagcgcc
      601 ggcgccgccc cagcccgaca cgatgctgca ggattcgcag ttgaggcagg tcttgctctt
      661 cccctcaacg atggggcact tccgctcccg gattacccgt cctttgtcca cgatcaggac
      721 tttttttccg tgccgcacaa gctccatggc ggcgaaaact ccggcagggc cggatccgac
      781 gataatggcg tcatatatca tgagtagtcc gcaccccgaa gggcgcgatt gttccctccc
      841 cgtctggtcg tatgcggatt ccaccgccgc tagcggcgct ctctccgctg cctgccgctg
      901 ccggacaggc cccgcttcag cgctttcagt gtctccagat cttttccgga tacctttttt
      961 gccgaagagg ctgatgtctg gatgcggtcc atcttcacca ggacttcaat gcttctcaca
     1021 tccacgccga gttcctctgc aagccgggcg gaggttacct tcctgagcct gccgtcggcg
     1081 gaagagtgat cccgaagcca ggtccatgcc tgcctgtaaa gctcttccag gtactccgcg
     1141 cattcagggc agagaggcgt cttctcttgc tctcccccgt caagataggc cttcgtgcaa
     1201 tgctcgcaga ttttcagtgc cacggccgat cctcactcct ctgcatttcg tttctttccg
     1261 ggcggcggac cttccggtcc cagccgttct ttcacttcag ccacaaagct ctcgactctt
     1321 gagggagtat ggataaacgc cgggtcggag aggtccaggg tcataagccc gccgttggac
     1381 agccccgtct cggagcggac gaactcccgg aaggaagaat aggcatcgta gagggacttc
     1441 cagtagggtt cccctgcggc cagttcgtcc tcccggcccc tggcccgtat ccggcgcatg
     1501 atctcttcga agggacagga cagcacaatc atgagccggg gcatggcaag gtgcttcagc
     1561 agcgaatcat agagcatgag atacgtgccg aactggctgt cccggaagta tcccagccga
     1621 tagtacagcg aagcgtatat tttgtcgccg aagacgctcc tgtcaagaat atagttctca
     1681 gactcggagg cgcagagata ctgggcaaac cttgtgacca ggaaattcag ctgcatggga
     1741 aacgcccacc gccgctcgtg gtggaatctc cggagaagat cgtactggtc ccggaattcc
     1801 tcgggcataa cctgcaggcc gagacgatcc gacagcaggt tcaccacggt ggacttcccg
     1861 cttgccgtca ttccttcgac aatgacctga acccgtccca tatcgctcac ctcaggggat
     1921 gggcggtgcg agaacggccc ggttgcctcc cgccctcttc gcttccctca gggcgattcc
     1981 cgcccgtttc aaaaattcgt cattggacat ttccctgcgg tattcacaca ctccgaagct
     2041 gcagaagacc ccgcgccccg ggaagaaatg ggaatgacag accatgtccc gaatcttgtc
     2101 agccatctgg tgggcaaggt agccgctcat gggcgtcagg acggcgaatt caccgccgcc
     2161 gaaacgggcg aggaaatcag tcgggcggat tcggcttttg acagcagtga agaagtcccg
     2221 gaggaatttt ttccccccat cttcccccgc ctgctccatc agttccctga aaccgtcgat
     2281 atccatcagg atgacggaaa ggcttccccg gtatctgccg gccctctcca tctcggtgga
     2341 caaaagcctg ccgaacccct ccctgtcggg tataccgagg agctcgtcga tacccctgct
     2401 cctctcctga ggagccttct ccccggacgg atccggggcg gcttcggccg acggagcctg
     2461 gactgccgga acgaccgtct cccgaaggga caggagaagg acctgcctgc ccccgcgctc
     2521 gaggggaatg gcggaaagaa ggacgggaag gtcctcgccg gagggaagcg tgatggactg
     2581 cttcaactcc ctgacggagc ccttttcccc gattcctgaa tacagggtgt ttttcagtgc
     2641 cccgtagggg agaagcccca attcctcaag ggtttttcct tcaggccttt cgccggaagg
     2701 aaaaactttt tcatgaaaag cccggttgca ttcgatgatc cgcccttcgt ccctggttac
     2761 gataatgcca aggtcggaca ccagggagaa cagcctgcgg aatttttcct cctcggcccg
     2821 gactttccgc tccgcggccc gctgtcccgt cttctcgcac agcaccaggg aaacgcccga
     2881 tacctgtccg tcgggagaaa aaaccggtgc gaaggagatg gaaaaaaacc tgtcccccgc
     2941 ctcctggcct atctcggccc cttctccgga gagcgctttt tccacggcga gccggaaggg
     3001 aagtcccctg tctccttcgg gaagaagttc cagcatggac agccccgttt cgggaggagc
     3061 gttccacatg ggagccgcct ctctcctgaa gccctcggtc atgaactgga ggatacagtc
     3121 cctgtcggta acggccgcca ggagattatc ggcggaacag gagaatccgg ccgggatttt
     3181 cgtttccgcc accggctcat tctccgccgg agccgggatt tcatacccca cggcgagcat
     3241 ggagggttcg ccccggtact ctacaggagt gacggccatg tcgagccacc gttccgcccc
     3301 accttccgtc agcacgggaa aggaaaaccg tctttcccag agttcatccc cgccggcctg
     3361 ctccagcaat ttttcaacct cgacgcaata ccccggcgcc acgtagtccg tcaggagaaa
     3421 tcttggccgc tcccggagag ggaagccgaa gaacgcttct gccctcccgt tgaggtacgt
     3481 cacccttccc cccgagagaa tcgccagaag gaaaaccggg ttttccgaaa gagcgaggta
     3541 ccggcgctcc atatcccgga gagcagtctc ctcccgtttc caccgtgcgg cttctttctc
     3601 cagcctgatt gtcttctgga tcataaaatt gactgaatcg gttccttcat tgccgagggg
     3661 cttatcgaaa ctctcgctca tcggggttta ccccttcctt ccaaaaatgc tccatgaaat
     3721 cacttccgct gcagccgaaa atatccgccc gtacatccct tgacatcggt aatgcagatg
     3781 aaacccctgt ttttcgtgtg ggccgttatg acgggg
//
LOCUS       DAOTHD010000082         3810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_28422_length_3810_cov_3.914248, whole genome shotgun sequence.
ACCESSION   DAOTHD010000082 DAOTHD010000000
VERSION     DAOTHD010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28422_length_3810_cov_3.914248"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /gene="hisS"
                     /locus_tag="P5219_02270"
     CDS             <1..831
                     /gene="hisS"
                     /locus_tag="P5219_02270"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRX25612.1"
                     /translation="EVFRRLGLSRLEVVINSVGCPACRPDYRRRLTEYFSSRERDLCE
                     TCRDRMGRNPLRILDCKKESCRQVTEDAPSVYESLCGECREHFDAVRSSLDGLGAVYR
                     LDKRLVRGLDYYTKTAYEVLSGALGSQNAVCGGGRYDNLAEAIGGPHVPGVGFAAGVD
                     RILMVMEQEGCSFGKEPRLDVFGVALDFRSRAAILSVVDLLRKNGISADMDFNDRSMK
                     AQMKAASARNARLACILGEDELTRGTVAVKDLTTGEQKEIAGEGLAQAVKEMLSTLKG
                     "
     gene            864..2675
                     /gene="aspS"
                     /locus_tag="P5219_02275"
     CDS             864..2675
                     /gene="aspS"
                     /locus_tag="P5219_02275"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRX25613.1"
                     /translation="MEGTHRHFDGSWQRTTYCGMVSGRAGEEIPAAVNGWVRRRRDLG
                     GLIFIELWDHTGVIQVVFNPELTPEVHERAGDLRSEFVLAVRGKIRARPEGTANPSMK
                     TGNWEIVAEDFLLLSPAAPLPFEISEATDRVDENLRLRHRYLDLRRERMQRNLRIRHM
                     ISMFTRNFLSGEGFLEIETPMLTLSTPEGARDYLVPSRVNPGTFYALPQSPQIFKQIL
                     MISGFDRYFQIVKCFRDEDLRADRQPEFTQVDIEMSYLTEENIYSLMECYMKGLFKEI
                     LDVDIPVPFRRMPYWEAMDRFGSDKPDLRIPFEIADLAPVFAGAGFEPFRDILAAGGY
                     VRGLPLPGGASLSRKNLSDVEEKAKKLGARGLAAFQLKDGALKGPLVKFLNEGQQELL
                     VKKSGLQEGGALFVLAGSERREVSEILGSLRLDLAREHGLVASERWEFLWVVEFPLFE
                     KDEEARRWAAVHHPFTSPMAEDIALMDTDPGMVRSRAYDCVLNGNEVGGGSIRIHDPA
                     VQEKVFSCLSFTREDARARFGFLLDALASGTPPHGGIALGLDRLTMLLCGASSIRDVM
                     AFPKTQEAQCLMSGAPSSVSKDQLDELRICSVSEEVK"
     gene            2739..3425
                     /locus_tag="P5219_02280"
     CDS             2739..3425
                     /locus_tag="P5219_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRX25614.1"
                     /translation="MVRLRYLGHAAFELKGEGTTILVDPFLSGNPYAAAGPGDFETVD
                     FIFVTHGHGDHIGDTVEIASRTGATVASNFELSHYFGGKGLKCHPMHIGGAFVFPFGR
                     VKMTPALHGSDITEGDHLIPGGSPCGFLFDMGGVKIYHAGDTGLTMDMQLLALEGVKA
                     ALLPIGGNFVMDVKDAARAVGFIHPEIAVPIHYDTFPVIKADPEEFSRLVGTLAKVVV
                     MKPGDLLELA"
     gene            complement(3483..>3810)
                     /locus_tag="P5219_02285"
     CDS             complement(3483..>3810)
                     /locus_tag="P5219_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRX25615.1"
                     /translation="DIVVVCELPSYVIEVMESIEKIAANGATVITITDSPAAPVCQLS
                     DLTFFVSDTSPTFGSTIVGPVFLVHILTSVLCVNLGEKVRAALQDQEMGLHDERIYYP
                     AYGLRY"
BASE COUNT          818 a         1051 c         1148 g          793 t
ORIGIN      
        1 gaagttttca ggcggctcgg cctgtcccgg ctcgaggtgg tcatcaattc agtgggctgc
       61 ccggcatgcc ggcccgatta caggcggcgc ctgaccgagt atttctcttc cagggagaga
      121 gacctgtgcg aaacctgccg cgacaggatg ggcaggaacc ccctgaggat tctcgactgc
      181 aagaaggaat cctgccggca ggtcacagag gacgctccat cagtgtacga gagtctctgc
      241 ggtgaatgca gagagcattt tgacgccgtg aggtcatccc ttgacgggtt gggggcggta
      301 taccgcctcg acaagcggct tgtcaggggg cttgattact acacgaaaac cgcatacgaa
      361 gtgctctccg gagccctggg atcccagaat gccgtgtgcg gcggaggccg gtacgacaac
      421 cttgccgaag ccatcggcgg gccccatgtt cccggcgttg gattcgccgc cggagtggac
      481 cggattctca tggtcatgga gcaggagggg tgttccttcg ggaaagaacc caggctcgac
      541 gtgttcggcg tcgccctgga tttccgctcg agagccgcta tcctttccgt tgtggacctg
      601 ctcaggaaaa acggtatctc tgccgatatg gatttcaatg atcggagcat gaaagcccag
      661 atgaaggcgg cctcggcccg aaacgcacgt ctggcctgta tcctcggcga ggatgaactg
      721 acccggggaa cggtggcggt caaggacctc actacgggcg aacagaagga aatcgccgga
      781 gaaggtctgg cgcaggcagt aaaagaaatg ctctctaccc ttaaagggta aagaaaccgg
      841 catgttgtga gaggaagggt cccatggaag gcacacatcg tcatttcgac ggcagctggc
      901 agcgaaccac ctattgcggt atggtctccg gcagggcggg agaagagatt ccggcggcag
      961 tgaacggatg ggtccgcaga aggcgggatc tcggaggtct catattcatc gagttatggg
     1021 accataccgg ggtgatccag gtagtcttca atcccgaact gactcccgaa gtccacgaac
     1081 gggcgggaga cctccgcagc gaatttgtgc tcgccgtccg ggggaagata cgggcccggc
     1141 cggaagggac ggccaatcct tccatgaaga ccggaaactg ggaaattgtg gcggaggact
     1201 tccttctcct gtcgcccgcc gcccctcttc cttttgaaat ttccgaggcc accgaccggg
     1261 tcgacgaaaa cctcaggctg cgccacaggt atcttgatct ccgcagggag aggatgcagc
     1321 gcaacctcag gatacggcac atgatctcca tgtttacgag aaacttcctc tcaggtgagg
     1381 gctttcttga aattgagact cccatgctga ctctctccac ccctgagggc gccagagact
     1441 accttgtccc gagccgggtg aatccgggca ctttctacgc gctgccccaa tcaccccaga
     1501 tttttaagca gatccttatg atcagcgggt tcgaccgcta tttccagata gtgaaatgct
     1561 tccgggacga agacctccgc gccgaccgcc agcctgagtt cacccaggtg gacatcgaga
     1621 tgagctacct gacggaggaa aacatctact ctctcatgga atgctacatg aaggggctct
     1681 tcaaggaaat tctggatgtg gacatccccg tccccttccg cagaatgccc tactgggagg
     1741 ccatggaccg tttcggaagc gacaagccgg acctccgtat tccctttgaa atcgcagacc
     1801 tcgctcccgt ttttgccggg gccggcttcg aacccttccg ggatatcctt gcggccggag
     1861 ggtacgtcag ggggcttcct cttcccggcg gcgcttcttt gtcgagaaag aacctttccg
     1921 acgtggagga gaaggcgaaa aagctcggcg caagggggct tgcggctttc cagctgaaag
     1981 acggcgccct caagggcccc ctcgtgaaat ttctcaacga agggcagcag gaacttcttg
     2041 taaagaaatc ggggcttcag gagggaggcg ctctcttcgt ccttgcgggc agcgagagga
     2101 gggaagtgtc ggaaattctc ggttcccttc gccttgacct cgccagggaa catggcctcg
     2161 tcgcttccga gcggtgggag tttctctggg tcgttgaatt tcccctcttt gaaaaagacg
     2221 aagaagcccg gcggtgggct gcggtccacc accccttcac ctcgcccatg gcggaggaca
     2281 tcgccctcat ggatacggac cctggaatgg tgagatccag agcctacgac tgcgtattga
     2341 acgggaacga agtcggcggc ggttccatca ggatccatga tccagcagtc caggaaaagg
     2401 ttttttcatg tctttccttc accagggaag atgcccgggc ccggttcggg tttcttcttg
     2461 acgctctcgc ctcagggacg ccgccccacg ggggaattgc cctcggactg gacagactga
     2521 ccatgctcct ctgcggagca tcctccatac gggacgtcat ggccttcccg aaaacccagg
     2581 aagcccagtg tcttatgtcg ggggcccctt cctcggtctc gaaggatcag ctcgacgaac
     2641 tgaggatatg ctctgtttcg gaagaggtca aatagagagg catcaacggg ctcccctgtt
     2701 caggggagcc cgcaatcata caaaggagga gatgaagaat ggtccggctt cgttacctcg
     2761 ggcacgcggc ctttgaactg aagggagagg gaaccaccat actggtggat cctttcctga
     2821 gcggcaatcc ttatgcggca gccggccccg gtgatttcga gacggtggat tttatttttg
     2881 ttacccatgg ccacggagac catataggcg atacggtcga gatcgcttca aggacgggtg
     2941 ccactgttgc ctccaacttt gagctcagcc attatttcgg agggaaaggg ctgaaatgtc
     3001 atcccatgca catcggaggc gcttttgtct ttcctttcgg cagggtgaag atgacgccgg
     3061 ccctccacgg ttcggatata acggaagggg atcacctcat tccgggaggc agtccgtgcg
     3121 gctttctttt tgacatgggg ggagtgaaaa tctaccacgc cggagacacc gggctgacca
     3181 tggatatgca gcttctcgcc cttgaggggg tgaaggcggc cctgctgccc atagggggca
     3241 acttcgtcat ggacgtgaaa gatgctgcac gggccgttgg gttcatccat cccgaaatag
     3301 ccgtaccaat tcactacgac actttccccg tgatcaaagc ggatccggaa gagttttccc
     3361 gtttggtggg aaccctggca aaagtggtgg ttatgaaacc cggagattta ctggaactgg
     3421 cctgagacga aactgccata aatgaatgag agggacgaca ggcgtccctc tcatttacga
     3481 tttcagtatc ttaaaccgta tgccggataa tagattcttt cgtcgtgaag gcccatttcc
     3541 tggtcctgaa gggcggcgcg gaccttttcg cccaggttca cgcagaggac cgacgtgagt
     3601 atgtgaacga ggaaaacagg gcccacgata gtgctgccga aggtgggact ggtgtcgctt
     3661 acaaaaaacg taaggtcgga gagctggcag accggagccg ccgggctgtc ggtgatggtg
     3721 atgaccgttg cgccgttggc ggcgattttc tctatggatt ccattacctc gatgacatag
     3781 ctcggcagtt cgcacaccac cacaatatca
//
LOCUS       DAOTHD010000083         3794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_28576_length_3794_cov_3.315058, whole genome shotgun sequence.
ACCESSION   DAOTHD010000083 DAOTHD010000000
VERSION     DAOTHD010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28576_length_3794_cov_3.315058"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="P5219_02290"
     CDS             complement(<1..661)
                     /locus_tag="P5219_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRX25616.1"
                     /translation="MSGYTEGILILLCINAIAAMGVSLLTGFTGIFSLGHAAYMALGA
                     YTTAILTVQHGVHWLPAILLGGVVAVAVAYFIGVPTLKLMGDYYAIASIGLGEAIRLI
                     LENWQSVTRGARGYPGIDPYTTLPVALAFFVVLAVFMFNIINSRFGRACKACRDDYIA
                     SSLLGFHTARIRVLSLMISAFYCGVSGALLGGFLSFIQPVMFDMLKSTELTAVVVFGG
                     LG"
     gene            complement(674..1555)
                     /locus_tag="P5219_02295"
     CDS             complement(674..1555)
                     /locus_tag="P5219_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRX25617.1"
                     /translation="MATLIQQVINGLSLGSVYALIAVGYSLVYSILLFSNFAHGGFLV
                     IGGYICYGTLRTFGTGIWMSGALALAGAGLAAVLTERLAYRPIRERTNVTLYLLIASM
                     GMSIVIENIFVIVAGGRFRALPPVIPTAPVNLMGLATTSAFDLLSLVSAVVFLGALQL
                     FLSKTRWGLAIRAAAYDLRTAGLMGVNVNLLISIVFFVAGALAAVGGIFLSVRYTLYP
                     QLGAITIKAFVAAVIGGLGSLPGAVVGSLILGLAEMLTAGFISSQMRDLVVFSMLVIT
                     LLVKPTGLFGKSVSEKV"
     gene            complement(1676..3049)
                     /locus_tag="P5219_02300"
     CDS             complement(1676..3049)
                     /locus_tag="P5219_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRX25618.1"
                     /translation="MKRNLLIGLAVLVVIAGYFMMQQKPAPEAPKETPKQEAPAPKPA
                     EAPKAEEKPAEAPKPAEAPKAEEKPADAPKPAEAPKPEEKPAEPAKTEEPKADAAPVK
                     VGYLAALTGDWAAYGQTEEKTARLAVEQINAKGGVLGRPIELVVYDFRTRAEDAVNAV
                     RRMIEEDKVVAIVGANGSGINIATAPVVNRLGVSQVGTVSTNPLVTVDEKGAVRPYSF
                     RICFTDPYQGKLIAFFAAKELKKMNAAILYDVGNDYSQGLREFFMKSYGEYGGKVVAD
                     LGFRGGQDVDFRAQLTEIRDKAADVLVLPNMGKEMALIMKQARELGLNEIVFVGGDGY
                     GEFMWEIAGEAMEGSYWINHVAPEDPAMQPFFAQYKERWNDECKEFVNGVLGYDSVYW
                     VADAIARAGSDDPKAIRDALEATKDLKLHHATITMDPATHNPVDKDGVVLIAKGGKGQ
                     FFTKIKP"
     gene            complement(3202..>3794)
                     /locus_tag="P5219_02305"
     CDS             complement(3202..>3794)
                     /locus_tag="P5219_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25619.1"
                     /translation="DFAELLTRARNRNPDLEELTERFLRSLPPSLDERERCVETASYL
                     LDLAGEKGPLIVTGFLPPYYPPRLNSGKTGGEQAVLRAAARLREDGKAFGMDITVGDV
                     FHGIMDLSYFGFQGAPGDLDALRDNMPLWGKEYRFPLDDLKKIDIPVANFGPVGKDDH
                     KNGERIHLPYFLDIFPGLFRNFARYLAEEAGKAAAD"
BASE COUNT          772 a         1129 c         1152 g          741 t
ORIGIN      
        1 agcccaggcc cccgaagacg accacggcgg tgagctcggt ggacttgagc atgtcgaaca
       61 tgaccggctg gatgaaggag agaaatcctc cgaggagcgc cccggacact ccgcagtaga
      121 atgcggagat catgaggctg agcacccgta tccgtgccgt gtggaacccc agcagcgacg
      181 aggcgatgta gtcgtcccgg caggctttgc atgcccttcc gaagcggctg ttgataatat
      241 tgaacatgaa gaccgccagt accacgaaaa aggccagggc caccggcagg gtggtgtagg
      301 ggtcgatccc gggatatccc cgggcgcccc gggtcacgga ctgccagttc tccaggatga
      361 gacggatggc ttccccgaga ccgatggagg cgatggcgta atagtcgccc atgagcttca
      421 gggtgggaac gccgataaag taggcgaccg cgacggcgac gacccctccg aggaggatgg
      481 ccggaagcca gtggacgccg tgctgaaccg tcaggatggc ggttgtgtag gcgccgagag
      541 ccatataggc agcgtggccg agggaaaaaa tccccgtgaa gccggtgaga agcgatacgc
      601 ccatggcggc gatggcgttg atgcagagga ggatgagaat tccttcagta tatccggaca
      661 tggtgggcgc cacctacact ttctcgctga cgctcttccc gaaaagcccg gtgggcttga
      721 cgagcagggt gatcaccagc atggagaaaa cgaccaggtc cctcatctgg ctcgagatga
      781 aaccggcggt aagcatctcg gcaaggccga ggataaggct gcccacaacg gccccgggca
      841 gggatccgag gcccccgatc accgcggcga cgaaggcctt gatggtgatg gcgccgagct
      901 ggggatagag ggtataccgc acggaaagaa agatgccgcc gacagccgcc agggccccgg
      961 cgacgaaaaa gacgatggag atcagcaggt tcacgttcac tcccatgagg ccggcagttc
     1021 gcagatcata ggcggcggcc cggatggcga gtccccacct ggtttttgaa aggaagagct
     1081 gcagagctcc gaggaaaacc actgccgaaa caagggagag aaggtcaaag gcactggtgg
     1141 tggctaaccc catgaggttc acgggagccg tcgggataac cgggggaagg gcccggaaac
     1201 ggcctcccgc cacaatgacg aagatgtttt cgatgacgat gctcatgccc atggaggcga
     1261 tgaggagata cagggtaaca ttggtccgtt cacggatggg acggtaagcg aggcgttcgg
     1321 tgagaacagc ggcaagtcct gcaccggcga gggccagagc cccggacatc cagatgcccg
     1381 ttccgaaggt gcgaagcgta ccgtagcaaa tgtagcctcc aatgacgaga aaaccgccgt
     1441 gagcaaaatt ggaaaataaa aggatggaat acacgagaga atagccgacg gcgatgagtg
     1501 catagaccga ccccagggaa aggccgttga taacctgctg gatcagcgtc gccaaagacg
     1561 gatcacccct ttcacgtatt gatgagggcg aacggaggtc cgccctcatc agctaaacta
     1621 tatcataccc ggatcttaat gtatactgta ttttccgaat acgccgtcag gtgcgctacg
     1681 gcttgatctt cgtgaagaac tgccccttgc ctcccttggc gatgagcaca actccgtcct
     1741 tgtccacggg gttgtgggtg gccggatcca tggtgatcgt cgcatggtgg agcttcaggt
     1801 ccttcgtcgc ctccagggcg tcacggatcg ccttcgggtc gtccgatccg gcgcgggcta
     1861 tggcgtcagc cacccagtac accgagtcgt atccaagcac tccgttcacg aactccttgc
     1921 attcgtcgtt ccaccgctcc ttgtactggg cgaagaaggg ctgcatggca gggtcttccg
     1981 gcgccacgtg gttgatccag taggatccct ccatggcctc tcccgcgatc tcccacatga
     2041 attcgccgta cccgtcgccg cccacgaaca cgatctcgtt cagaccaagc tcccgggcct
     2101 gcttcatgat cagcgccatt tccttgccca tgttcggcag cacaaggaca tcggccgcct
     2161 tgtcacggat ctccgtcagc tgggcacgga agtccacgtc ctggccgccc cggaacccga
     2221 gatcggcaac caccttgccg ccgtattcgc cgtagctctt catgaagaac tcacgaagtc
     2281 cctgggagta gtcgttcccc acgtcgtaca ggatcgccgc gttcatcttc ttcagctctt
     2341 tcgcggcgaa gaaggcgatg agcttgccct ggtacgggtc ggtgaagcag atccggaagg
     2401 agtacggacg gaccgctccc ttctcgtcca cagtcacgag ggggttggtg gacacggttc
     2461 cgacctggga tacaccgaga cggttcacta caggggccgt ggcgatgttt attccgcttc
     2521 cgttggcccc gacgatggcg acgaccttgt cctcttcgat catccggcga accgcgttca
     2581 cggcatcctc ggcccgggtg cggaaatcat agaccacaag ctcaatggga cggccgagca
     2641 cgccgccctt ggcgttgatc tgctcaaccg cgaggcgggc agtcttctct tccgtctggc
     2701 cgtaggcggc ccagtcgccc gtaagggcgg caaggtagcc gacctttact ggagcggcat
     2761 ccgctttggg ttcttcagtc ttcgccggtt cggcgggctt ttcttccggt ttcggagctt
     2821 cggcgggttt cggagcgtca gcaggctttt cctcggcctt gggtgcctct gccggcttcg
     2881 gggcttctgc gggcttttcc tccgccttgg gcgcctcggc gggtttcggt gcgggagcct
     2941 cctgcttcgg agtttccttc ggcgcttcgg gcgccggttt ctgctgcatc atgaaatacc
     3001 cggcaatcac aacgagaaca gccaaaccta tcaacaagtt ccgtttcaca atccaccacc
     3061 tcctgagttt ttcgctgcct gaacggttct tattttacta cgaccatgtt ccggccacaa
     3121 actgtttcct ttttccgccc gctttctcct ttccgtttcc gccggtgaaa agggcaccgg
     3181 aacagccggt gccctccgct gtcaatccgc tgcggccttt cccgcttctt cggccaggta
     3241 gcgggcgaag ttccggaaca gccccggaaa aatgtccagg aaatagggga gatggatcct
     3301 ttctccgttc ttgtggtcgt ctttgcccac gggaccgaaa ttcgccacgg gaatatcgat
     3361 ttttttcaga tcatccaggg gaaaccggta ttcttttccc cagaggggca tattgtccct
     3421 cagggcgtca agatcgccgg gagctccctg gaagccgaag tagctcaggt ccatgatgcc
     3481 atgaaagaca tccccgacgg taatgtccat gccgaaggct ttcccgtcct cccggagacg
     3541 ggcagcggcc cggagaactg cctgttctcc gccggttttc ccgctgttca gcctcggtgg
     3601 ataataaggg ggaagaaacc ccgtcacgat cagcggacct ttctctcccg ccaggtcaag
     3661 cagataggag gctgtttcca cgcaccgctc ccgctcgtcg agggaaggcg gaagggagcg
     3721 gaggaacctc tccgtgagtt cctcgagatc gggattcctg ttcctggccc tggtcaggag
     3781 ttctgcaaaa tcca
//
LOCUS       DAOTHD010000084         3776 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_28755_length_3776_cov_3.014243, whole genome shotgun sequence.
ACCESSION   DAOTHD010000084 DAOTHD010000000
VERSION     DAOTHD010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28755_length_3776_cov_3.014243"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="P5219_02310"
     CDS             complement(<1..947)
                     /locus_tag="P5219_02310"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRX25620.1"
                     /translation="MVRLEKVILDNLRLEPEKPCLWWEGKWWTNGDLLRLVEESTKGL
                     EKGGFRAGYRLAVLLPNSPLVLALSLAAWNLGGAISPLNAKSGLPSLMGTLDLVEPCA
                     VVVAPGLDELKTALEEQDVPVVVAESLSAALPACTVKETSLENDSLAVIFATSGTTGM
                     PKAVPLTHANLLDNSLMMYDALELLQEGDILLNVLPNFHSFGYTVAGLMPLVKKLRQT
                     LVASFLPPVETMKTIHDSGTNVIVAVPTMLYFMTVAASKGASMPVSLRMIVTGGDRLN
                     VQLDGKVKEVFGVGIIEGYGLTECSPVVAVNRNYKDRRIG"
     gene            complement(1102..1872)
                     /locus_tag="P5219_02315"
     CDS             complement(1102..1872)
                     /locus_tag="P5219_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25621.1"
                     /translation="MEGDVMNRVFTKGFILLAVFLVVLAGLSAAMLLLTPEEGRTGNF
                     WISFWTIIFAVVLAFVYMLFHIFAGREGTAPLPLLLGISATLALYCAFVLGNVAVSHY
                     FLGLSRNAYLATHILGFLVLAGGGGALTILSLSTKEADTTASLKRSRLFVLTTRIGSL
                     AGELNLCSHREMASGIIGGLNDLKEAIRFSDPMSAGGEDGEEKVVLAVAALENKCRRF
                     MALSSGDEREKAVLEIENLIDRAFSALKTRNEDVLHGK"
     gene            complement(1873..3048)
                     /locus_tag="P5219_02320"
     CDS             complement(1873..3048)
                     /locus_tag="P5219_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRX25622.1"
                     /translation="MAMIQVVSFDSGLDPHEILAWRYRDPGSPGKSDELGTWAQLVVD
                     ESQEAVLYRNGQALDLFGPGRHTLSTANIPLLSKVVNLPYGGESPFKAWVWFVNRVHS
                     LDVKWGTASPIQLQDPKYGVLLPVRSYGQFGIRIADSRKFLVKLVGAASQLDREMLVK
                     HFRGLLLTRIHDLISTYLIHKKISVVEVNAWLDEISGEMGDRVAPLFREYGVEVLNFF
                     VNSINVPEEDPAVAELKKTLTERAKMEVLGYSYQQMRSFDALEKAASNEGGGNLINAG
                     LGLGVGVGMGGAFGRMAEQMGRNLSAPDSSRTCGKCGAPVSEGARFCSGCGAPMPGRT
                     VCPGCGASLSGGMKFCPECGMALSPRCPSCGKTASPGAKFCPECGTELTGKSNPEER"
     gene            3198..>3776
                     /gene="larB"
                     /locus_tag="P5219_02325"
     CDS             3198..>3776
                     /gene="larB"
                     /locus_tag="P5219_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HRX25623.1"
                     /translation="MDEQSLLSLIRRVKDGDLDEHELVRQIRAMPFRDLGEVKFDTHR
                     ALRKGFAEIVYCPGKSDSQIESIAAALAGTEDNVLFSRATAHQYGIVKNHLQDAVFHE
                     KARIIGAKRKDYPACPGLTVITAGSSDVPVAEEAAVTAEYMGCTVERLYDVGVAGLHR
                     LLAHVDVLQRSRAIVAVAGMEGALPSVVGGLVS"
BASE COUNT          776 a         1161 c         1163 g          676 t
ORIGIN      
        1 ccgatgcgcc ggtccttgta gttccggttc accgccacaa cgggagagca ctcggtgaga
       61 ccgtaccctt cgatgatgcc gacgccgaag acttccttca cttttccgtc gagctggacg
      121 ttcagccggt ctccgcccgt gacgatcatc ctgagggaga cgggcatgga ggcgcctttg
      181 gaggcggcca ctgtcatgaa atagagcatg gtcgggacgg ccacgatcac gttggtgccg
      241 gagtcatgga tggtcttcat ggtctcaacg gggggaagga aggatgccac aagggtctgc
      301 cggagctttt tcaccagggg catgaggccg gccacggtgt agccgaagga atggaagttc
      361 ggcagcacgt tgagaaggat gtcaccttcc tggagaagct caagggcatc gtacatcatc
      421 agggagttgt ccagcaggtt ggcgtgggtg aggggaacgg ccttgggcat tcccgtggtc
      481 ccggaggtgg cgaagatcac cgccagggag tcattctcca gggaggtttc tttcaccgta
      541 caggccggca gggctgcgga aagggattcc gccaccacca cgggaacatc ctgttcctca
      601 agggccgtct tcagctcgtc gaggccgggg gcgacgacca cggcgcaggg ttccacaagg
      661 tccagggtcc ccatgaggga cgggagcccg gatttcgcgt tcaggggcga aatagcccct
      721 ccgaggttcc aggccgcaag ggacagggcc agcacgaggg gagagttcgg caggagcacc
      781 gccagcctgt acccggcgcg aaagccgcct ttttcaagac ccttcgtcga ctcttccacg
      841 agccggagca gatctccgtt ggtccaccac tttccctccc accagagaca gggcttctcc
      901 ggctcgaggc gcaaattgtc caggattacc ttttcaagtc tcaccataga attctccccc
      961 ttcggtttat tctcaagagt gcgtcggaac cgcacatatc accagcgggt gacagaggat
     1021 attgtaaagc aaaaagagcc ctccgtgcga aaaaaaacgc ggaaggctcc caaaaaacag
     1081 aagaaaaggg gaaaataata gctacttgcc gtgaaggaca tcctcgtttc gtgtcttgag
     1141 ggcactgaac gccctgtcga tcaggttttc gatctccagc accgcctttt ccctctcatc
     1201 tccggaggaa agggccataa agcggcggca cttgttctcc agcgccgcta cggcgagcac
     1261 caccttctct tctccgtcct cgcccccggc gctcatggga tcgctgaagc ggatggcctc
     1321 cttcaggtca ttgagcccgc cgatgatgcc ggacgccatc tccctgtggg aacagaggtt
     1381 cagctccccg gccagggaac cgatcctggt ggtgagaaca aagagcctgc tcctcttgag
     1441 ggaggccgtg gtgtccgcct cctttgtgct gagggacagt atggtgagtg cccccccgcc
     1501 gcccgccagc accaggaacc cgaggatatg ggtggccagg taggcgttcc tggagaggcc
     1561 gagaaagtag tgggagacgg ccacgtttcc gaggacgaaa gcgcagtaca gcgccagcgt
     1621 agccgatatt ccgagcagca ggggaagagg ggccgttccc tcccgtccgg cgaagatgtg
     1681 gaaaagcatg tagacaaaag cgaggaccac cgcaaaaatg atcgtccaga aggaaatcca
     1741 gaaatttccg gtccttccct cctccggggt gaggaggagc atcgccgccg aaagccccgc
     1801 cagcaccacc aggaaaacag caagcagaat gaaccctttc gtgaataccc tgttcatgac
     1861 atcaccttcc atctatcttt cttccggatt gctttttccc gtcagttcgg tcccgcattc
     1921 cgggcagaat ttcgcaccgg gggaggccgt tttcccgcag gagggacacc ggggcgacag
     1981 ggccatgccg cattccgggc agaacttcat ccccccggaa agggaagcgc cgcacccggg
     2041 acagacggtc ctgccgggca tcggggcacc gcatccggaa cagaacctgg ccccctcgga
     2101 tacaggggcg ccgcacttcc cgcaggtccg ggaggaatcc ggtgccgaaa ggttccggcc
     2161 catctgctcc gccatcctgc cgaaggcgcc gcccatgccc acgccgaccc cgagcccgag
     2221 ccctgcgttg atgaggtttc ctcccccttc gttggaagcc gccttctcca gggcgtcgaa
     2281 ggagcgcatc tgctggtagc tgtagcccag cacctccatc ttcgcccgct ccgtgagggt
     2341 tttcttcagc tcggcgacag ccgggtcctc ttccgggaca ttgatggagt tcacgaagaa
     2401 attgagcacc tccacgccgt attcccggaa cagaggggcc accctgtccc ccatctcgcc
     2461 ggagatctcg tcgagccagg cgttgacttc caccacggaa attttcttgt ggatcaggta
     2521 ggtggaaata aggtcatgga tccgggtgag cagaagcccc cggaaatgct tcaccagcat
     2581 ctcccggtcg agttgggatg cggccccgac gagcttgacc aggaatttcc gggaatccgc
     2641 tatgcggatg ccgaactggc cgtaggagcg caccggcagc aggacgccgt acttcgggtc
     2701 ctggagctgg atgggagagg cagtgcccca cttcacgtcg agggagtgga cccggttgac
     2761 gaaccacacc catgccttga agggggattc ccctccgtag ggaaggttca ccaccttcga
     2821 gagaagggga atgttggccg tggacagggt gtgccttccc ggaccgaaga ggtccagagc
     2881 ctgcccgttc ctgtagagga ccgcctcctg ggactcgtcc accacaagct gggcccacgt
     2941 gcccagttca tcgctctttc cggggctgcc cgggtccctg taccgccagg ccaggatctc
     3001 gtggggatcc agcccggaat cgaaactcac aacctgaatc atcgccatgg tcgagcgcac
     3061 cgcctttcat tccgagtccc ccggcagtca cccctctttt cgggggtaaa cccaagggtg
     3121 cggaaatata tgctaccata tcttgccgga gatggagcat tcgagaaccc aacagggacg
     3181 aaaggacgga gcggacaatg gatgaacaga gccttctttc cctcatccgc cgggtgaagg
     3241 acggagatct cgacgaacat gagctggtgc ggcagatacg ggcaatgccc ttccgggacc
     3301 tcggggaggt aaaattcgac acccacaggg ccctccggaa agggttcgcg gagatcgtct
     3361 actgccccgg aaagagcgat tcccagatcg agtccatcgc cgcggccctc gcaggaacgg
     3421 aggacaacgt cctcttttcg agggctacgg cccaccagta cggaattgtc aaaaaccacc
     3481 tgcaggacgc cgtattccac gaaaaggccc ggatcatcgg agcaaagagg aaggactacc
     3541 ctgcctgccc cggcctcacg gtcatcactg ccggcagcag cgacgttccc gtggccgagg
     3601 aggcggcggt caccgccgaa tacatggggt gcacggtgga acggctgtac gacgtggggg
     3661 tggcggggct ccacaggctc ctcgcccatg tggacgttct gcagcggtcc agggccatcg
     3721 tggccgtggc gggcatggag ggagcccttc ccagcgtcgt tggggggctg gtctcc
//
LOCUS       DAOTHD010000085         3756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_28970_length_3756_cov_3.380168, whole genome shotgun sequence.
ACCESSION   DAOTHD010000085 DAOTHD010000000
VERSION     DAOTHD010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28970_length_3756_cov_3.380168"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="P5219_02330"
     CDS             complement(<1..1004)
                     /locus_tag="P5219_02330"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRX25624.1"
                     /translation="MALMETRLSKAVLGINPSPTEATSSLANRMRRDGIDIISFAQGE
                     PDFDTPPDISLEAVRAVVEGYTRYTDVPGMRDVREAVAEKFGRENGIRYTPSEVMISN
                     GGKQILYLLFRTICDPGDEVVVPTPCYVSYEEQIRLSGASPVFAPVSEDRSFRIARDD
                     IEPFITPRTKAVILNSPNNPTGAVCEEGNLRAIAALAAERGIFIVTDEVYEHLLYDRR
                     KHVSIAALGEEAKRNTVTVNSASKTYAMTGWRIGYAGGPEDVISGMITLQGHVSGNVC
                     SVAQRALLQALRGPQDAVAAMRDSYARRRDLMVEKIGSIPGLRCRKPDGAFYVFADVT
                     G"
     gene            1431..2441
                     /locus_tag="P5219_02335"
     CDS             1431..2441
                     /locus_tag="P5219_02335"
                     /inference="COORDINATES: protein motif:HMM:NF040043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HRX25625.1"
                     /translation="MFKDSWQFSRSRSKSEKFLEGAIDLHVHPGPHLFSSPRSTDPIK
                     TAIEARDAGMRAFAIMDVFNMSVGTAWMVQQVVEGIEVYGGIILNTVFGGMNPRAVKT
                     AMYYGSGARYVTFGAHSTYFQAYKEGRYDDDGKWILLREKYPKFVTEELDRCIRIPEG
                     EPTPELKEILEVIAANPQVYLETGHVSNQEAFRLLELGKQYGIKKLVVSNAVVENMSD
                     AEIQRAIDMGGWIERLLAAHTHTTPIPKTHYYVEPEYRAMDEGLQSTTHGGVAKVAEQ
                     IKKFGADHFFIGTDFGVYTLPTPVEGFREFIACLMDLGLSDEEIRKVSSINPGKLVDL
                     ND"
     gene            2516..3481
                     /locus_tag="P5219_02340"
     CDS             2516..3481
                     /locus_tag="P5219_02340"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRX25626.1"
                     /translation="MLTKRTMLLGTAVLLAVLSFGAMASAADFPKAKQLRMVVPYGAG
                     GGVDISTRILATPAQDILGQRIDVVCMPGAGGQEGINFVLQQPRDGYTLLVTDYGPLV
                     TTALTEEVTYDLSDWVPLVQITEVVPTFFVLAESPLKNVEDWVKAAKEKPESLTVAHG
                     RHLSVPHLPLILFEKTAGIKNTHVPTTGGSEALAYVLGKKVDIGASVPSTIASTVKAG
                     TMRPLAVCSLERVATLPDTPTMKELGYDVVLPAWYTVFAPKGVPEETLKILEEKFIEA
                     LKSEGAKEMAGKSNVLLTPYGRDKTAGLYEGTISSLKTILADVKK"
     gene            3571..>3756
                     /locus_tag="P5219_02345"
     CDS             3571..>3756
                     /locus_tag="P5219_02345"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRX25627.1"
                     /translation="MDELFFQVLEGAFALPVILYTTVGVFLGITVGAIPGLSGDMAIA
                     LLLPLIYKVSPAAALGML"
BASE COUNT          823 a         1135 c         1059 g          739 t
ORIGIN      
        1 ccggtgacgt cggcgaaaac ataaaacgcc ccgtcgggct tccggcagcg gagcccggga
       61 atagagccga tcttctccac catcaggtct ctccgccggg cataggaatc ccgcatcgcg
      121 gcgacagcgt cctggggccc ccggagagcc tgcagaaggg ccctttgggc gacggagcac
      181 acgtttcccg acacatggcc ctggagggtt atcattcccg agatgacgtc ttccggacct
      241 ccggcatagc cgatccgcca tcccgtcatg gcataggtct tcgatgcgct gttgacggtc
      301 accgtatttc tcttggcctc ttcgcccagg gccgctatgc tgacatgctt tcggcggtca
      361 tagagaaggt gttcgtacac ttcatccgtt acgatgaaaa tgccccgttc cgccgccagg
      421 gcggctattg cgcggaggtt gccctcttcg cagaccgctc cggtcgggtt gttgggggaa
      481 ttcaagatga cggcctttgt tctcggggta atgaagggtt caatatcgtc gcgggcaatt
      541 ctgaagctgc ggtcttcact gacgggggca aaaacgggag aagcgcctga aaggcggatc
      601 tgttcctcgt agctcacata acacggcgtg ggaacgacga cttcatcccc cggatcgcag
      661 atggtccgga aaaggaggta gaggatctgc ttccctccgt tggagatcat cacctcggag
      721 ggggtgtacc tgattccgtt ttccctcccg aatttttccg ccaccgcttc cctgacgtcc
      781 ctcatcccgg gaacgtcggt gtagcgggta tagccttcga cgacagcccg gaccgcttcc
      841 agggaaatgt ccggcggagt gtcgaaatcc ggttctccct gggcaaagct gataatgtcg
      901 attccgtcac ggcgcatgcg gttcgcaagg gaagacgtcg cttctgtcgg tgaggggttg
      961 attccgagca ctgcctttga gagcctggtt tccataaggg ccatcactcc atcgggtgtg
     1021 tagtgtggaa gcaaagattg cgccgatcac attttcgcgg cggcacttta ataaatagca
     1081 cggctgaaat ttagtgtcaa gtggttcatg gtatatgcat tttatattat gtgtatattg
     1141 caataaataa tgcaagtgct gcctcccccc aaaagaaatg cggaagagaa acaggccggc
     1201 acacaggggc aatgccgggg aggtccggac tcctgcggat ggagcggaag atgaaaaaaa
     1261 caccccgtac ttcccgcccc cgctgcggct gaccgggagc cgggcagcgt ctcgacccca
     1321 gcatttgacg tatagcatta aacatgatat atagtctgat gaatgagaaa agtgcttttt
     1381 tcatgtccgg tacccaaaat tctctttaca gctcccaggg aggcgaggca gtgtttaagg
     1441 attcatggca gttcagccgc tccagaagca aaagcgaaaa attcctcgaa ggcgccatcg
     1501 atcttcatgt tcatcccggt ccccatcttt tcagcagtcc gcggagcacc gaccccatca
     1561 agaccgcaat tgaagcccgt gacgccggaa tgcgggcctt tgccatcatg gacgtcttca
     1621 acatgtccgt gggaaccgcc tggatggtcc agcaggtggt ggagggcatc gaggtctacg
     1681 gcggcatcat cctgaacacc gtgttcggcg gcatgaaccc cagggcggtc aaaacggcca
     1741 tgtactacgg ctcgggagcc aggtacgtca ccttcggggc ccattccacc tactttcagg
     1801 cctacaagga aggccggtac gacgacgacg gaaaatggat cctcctccgg gaaaaatacc
     1861 cgaagttcgt caccgaggaa ctggaccggt gcatccggat ccccgaggga gaacccaccc
     1921 ccgagctgaa agagatactc gaggtcatcg cggccaatcc ccaggtgtat cttgaaacgg
     1981 gccacgtctc gaaccaggag gccttcaggc tccttgagct cgggaaacag tacggaataa
     2041 agaagctcgt cgtgtccaac gcggtggtgg aaaacatgag cgacgccgaa atacagagag
     2101 ccatcgacat ggggggatgg atcgaaaggc tcctcgcagc ccacacccac accaccccca
     2161 ttcccaagac ccattactat gtcgagccgg agtaccgggc catggacgag ggacttcagt
     2221 ccaccaccca cggcggcgtc gccaaagtgg ccgagcagat aaagaagttc ggcgccgacc
     2281 actttttcat cggcacggat ttcggcgtat acacgctgcc gacgcccgtt gagggcttcc
     2341 gggaattcat cgcctgtctc atggacctcg gcctttccga cgaggaaatc cggaaggtca
     2401 gcagcatcaa cccgggcaaa ctggtcgacc tgaacgactg acccataata attttgcggg
     2461 ggaagacccg tgaaacggta aagaaccaca ctacaccaag ggagggacgt tttttatgct
     2521 gacgaaaaga acaatgcttc ttggtaccgc agtgctgctc gcagtgctgt ccttcggggc
     2581 catggcctcc gccgccgatt tcccgaaggc gaaacagctc cgcatggttg tgccctacgg
     2641 agccggaggt ggggttgaca tctccaccag gattctcgcc actccggccc aggacattct
     2701 cggccagcgc atcgacgtgg tctgcatgcc cggcgcgggc ggccaggaag gcatcaactt
     2761 cgtgctccag cagcccaggg acggctacac ccttcttgtc accgactacg gccccctggt
     2821 gaccacggcg ctcactgaag aagtgaccta cgatctttcc gactgggtcc ccctggtcca
     2881 gatcaccgaa gtggtcccca ccttcttcgt cctcgccgaa tcccccctga aaaatgtgga
     2941 ggactgggtg aaggcggcga aggaaaagcc tgaatccctc acggtcgccc acggcagaca
     3001 cctgagcgtt ccccacctgc ccctcatcct tttcgagaaa accgccggga ttaaaaacac
     3061 ccacgttccc accaccggcg gctccgaggc ccttgcctac gtcctcggca aaaaagtgga
     3121 catcggcgct tccgtcccca gcaccatcgc ttccaccgtc aaggccggaa ccatgcgccc
     3181 cctggcggtc tgctccctcg agcgggtcgc cacacttccg gacacgccga cgatgaagga
     3241 actgggatat gacgtggttc ttcctgcctg gtacaccgtg ttcgccccca agggcgttcc
     3301 cgaggaaacc ctcaagatcc ttgaagagaa gttcatcgag gcgctcaaat cggaaggcgc
     3361 aaaggaaatg gcgggcaaat ccaacgttct cctgaccccc tacggccggg acaagacggc
     3421 aggcctctac gaaggaacca tttcgagcct caagaccatt ctcgccgacg tcaagaaata
     3481 gcggcgcgga cggacagcag aagagcggga caattctccg gagacgggaa ttcccgctct
     3541 tttttctgat acaaggagga cgaagcaacc gtggacgaac ttttttttca ggtacttgaa
     3601 ggggctttcg ccctgcccgt tattttatac acgacggtcg gcgtgtttct cgggatcaca
     3661 gtcggggcga ttcccggcct gagcggggat atggccatcg ccctcctgct tccgctgatc
     3721 tacaaggtgt cccccgccgc ggccctcggc atgctc
//
LOCUS       DAOTHD010000086         3728 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_29266_length_3728_cov_3.296760, whole genome shotgun sequence.
ACCESSION   DAOTHD010000086 DAOTHD010000000
VERSION     DAOTHD010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29266_length_3728_cov_3.296760"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3728)
                     /locus_tag="P5219_02350"
     CDS             complement(<1..>3728)
                     /locus_tag="P5219_02350"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRX25628.1"
                     /translation="KTVPKVIKPELPKKPAVPTPPPPSKLVKVGTGIIMIYQGYEALK
                     AYSDYLDKAAREDPENFGLLTVGKGIGYGVATLFGIPGLIQMGEQIGLSTREQYLKDL
                     REGKEWLLPPAVWAGAYAAWEFGKSVTIGPLISGGEAILEGYGLAGDLLDARKAAQRA
                     LDMKQRLDTAYKPLSDLMTHVIGGPLPPIWEKNRWKYYRIFKPILDDPEALDKLMKTP
                     AGKDLWKWMKEYKDKRAKELGITFGSPGADNPDDNDNIGKALLALPPQERGLYLRAAE
                     KGNTFVINFLSSRNRGAFAEPLGPETLGAFLQASKSDERVAFEAPFSEEQRAAAEEIY
                     RDLLKAVAETPDVAILSSGYAKGAAALLGQSEPATLLQPGEAGESLEKFRLLILPSGS
                     LSDWSLSETFRNAVEKYVSSGGTLLAFSQQLGKEYAVLPGKVTAYGFAEDQSCQYASA
                     RITASNGAFCSMTTPTPDFNVDGYFLDYPAESEIWLSRTKNDQPCMISFPWGKGRVVL
                     STLYLDWAAGNHQGTLHERLFFRDLVSFLLDKAPVLTHEGKTVRVALPEPETGDRGIS
                     FSPLLFGPDGAEIEVSPQPAAGKELDLSLSGVPGFYRLGGVFTDGSGKKTGETFPRTR
                     FGLSVLPKGISDAGNGQRNGVSVSVASDLENYIRGAEGRFSILLWNRGTEEKTVRIDW
                     RFPHNSGSAPGAEKEKYAGSNTLVLKPGARETINLAIPIVNTDGIDRLWVDVYDGRTR
                     EKISTVSKGFYTKSPSAEISVLLSGRTFPEGSALKGEVTFKNPFRARGTGTLRIRTAD
                     SAGKTAVDEEMDFEVSEDGATVPFQVSLPENTFSGECSLQAFVVLRGTVVGVGSASFT
                     YTGRPYALKGKITDRITGAPVSGSEVEFHLGDTSFKGKTDSGGIFEVDLPDGRYSIEA
                     SAEGFNIFTADAVIAPEDRAEYPITLLPLGKGEGTGMVSGRVFDRVTGDPVPGCLIDI
                     VRGNEKYTVKSDASARYSLALLPGDYLARCFVEGQSVNTSEFPFRVTEGWEQPLDLYP
                     PLGKIRLRILDTISGKEVGNVSAILRTTDLSKEDGWNRDISFAVNGGPTALTAPGRRA
                     LRVSADGYRMLETEILAGERAGETLLYLTPKKGSLKVVCADLVSEDPLAGAAVSAGRP
                     GQPASEMKKTGDDGGCIFSLEDGRWVFSVEAEGYVTLSTEWYSTVHAETEVPEVKLYL
                     SRDTQKKPGSARIAVLDRMTASPVAGVSVEVASPQGFQ"
BASE COUNT          688 a         1090 c         1112 g          838 t
ORIGIN      
        1 gctggaatcc ctggggtgag gccacttcaa cggaaactcc tgcaacggga ctggccgtca
       61 tcctgtcgag gacggctata cgtgcgcttc ccggtttttt ttgcgtgtcc cgggaaagat
      121 acagtttcac ctccgggacc tctgtttcgg cgtggaccgt cgaataccat tccgtggaaa
      181 gggtgacgta cccttccgcc tcgactgaaa aaacccatcg tccgtcctcg aggctgaaaa
      241 tgcatcctcc gtcgtcgccg gtctttttca tttcggaggc gggctgcccc ggacgccctg
      301 cggagacggc agcccccgcc aggggatctt cgctgacgag atcggcacag acgaccttaa
      361 gcgatccttt cttcggggtc agatagagaa gggtttcccc tgccctttcc ccggcaagga
      421 tttccgtttc aagcatcctg tacccgtcgg ccgataccct cagggctctc ctcccgggag
      481 cggtcagggc ggtgggaccg ccgttgacgg cgaaggaaat gtcccgattc cagccgtctt
      541 cctttgaaag atcggtggtc cggagaatgg cggaaacgtt ccccacctcc tttccagaga
      601 tggtgtcaag gatcctgagc cgtattttcc ccagcggggg atagaggtcc aggggctgtt
      661 cccatccttc cgtgacccgg aaaggaaact cagacgtgtt cacgctttgc ccttccacga
      721 aacagcgggc aaggtaatcg ccgggaagca gggccaggga ataccgggcc gaggcatcgc
      781 tttttaccgt gtacttttcg tttcccctga caatatcgat caggcatccg ggcacgggat
      841 cgccggttac cctgtcgaaa acccgtcccg agaccattcc tgttccctct cccttgccga
      901 ggggaagaag ggttatggga tattccgccc tgtcctctgg ggctataacg gcatcggcag
      961 taaaaatgtt gaagccctcg gccgaggctt cgatgctgta tcgcccgtcg gggagatcaa
     1021 cttcaaaaat tccgccgctg tcggttttcc ccttgaagga ggtatcaccg aggtggaatt
     1081 cgacttcgct tccggagacc ggagccccgg tgatgcggtc ggtgatcttt cccttcaggg
     1141 cgtacggcct gccggtgtag gtgaacgagg cgcttcccac tccgactacg gtgcccctga
     1201 gaacgacaaa ggcctggaga gagcactctc cggaaaaagt gttttccggg agggaaacct
     1261 ggaatggaac ggttgctccg tcctcggata cctcgaagtc catctcttcg tcaacggcag
     1321 ttttcccggc ggagtctgcg gtcctgattc tcagtgtgcc cgttcctctc gccctgaagg
     1381 gatttttaaa ggtgacctcg cccttcagcg cgcttccctc cgggaacgtc cggccggaaa
     1441 ggaggacgga aatttcggcg gaaggactct tcgtatagaa tcctttcgat actgtgctga
     1501 ttttttccct ggtccggccg tcatagacat cgacccacag gcggtcgatg ccgtcggtgt
     1561 tcacgatggg tatggcaagg tttatggttt cccttgcccc gggcttcagg accagggtgt
     1621 tcgagcccgc atatttttct ttctccgccc ccggtgcgct tccggagttg tgggggaacc
     1681 tccagtcgat ccggactgtc ttttcttctg ttccccggtt ccacaggaga atggagaacc
     1741 gtccctcggc ccccctgatg tagttctcca ggtcgctggc aaccgaaacg ctcacgccgt
     1801 tcctttggcc gtttccagca tctgaaatgc ctttcgggag gacactcagg ccgaagcgcg
     1861 tcctgggaaa agtctctccc gttttctttc ccgatccgtc ggtgaacacg ccgccgagcc
     1921 gataaaaacc ggggactccg gaaagggaaa gatccagttc ctttccggcg gcgggttgcg
     1981 gggagacctc tatctcggcg ccgtccgggc cgaaaagaag aggggaaaag gaaatcccgc
     2041 ggtcgcctgt ttccggttcc ggaagggcaa cgcggacggt tttgccctca tgggtgagga
     2101 ccggggcctt gtcgagaagg aaagagacaa ggtctctgaa gaagagccgc tcatggaggg
     2161 ttccctggtg gttgcccgcg gcccagtcaa ggtacagggt cgacagtacg acccgtcctt
     2221 tgccccaggg gaaagagatc atacagggct ggtcgttttt tgtcctcgaa agccatattt
     2281 cactttcggc cgggtagtcg aggaaatacc cgtccacgtt gaaatccggc gtaggggtcg
     2341 tcatggagca aaatgccccg ttcgaagcgg tgatccgtgc ggaggcgtac tggcagctct
     2401 ggtcttccgc gaatccgtag gcggtcacct tcccgggcag aacggcgtat tctttcccga
     2461 gctgctggga gaaagcgagc agggtccctc ccgaggaaac gtatttttcc accgcgttcc
     2521 ggaaggtttc cgagaggctc cagtctgaca gggatccgct cgggaggatg agcagcctga
     2581 acttttcgag ggattcgccg gcttctcccg gctgcagaag ggtggcaggt tcggactggc
     2641 cgaggagggc cgccgctccc tttgcgtacc cggaggaaag gatcgccacg tcgggtgtct
     2701 ccgccacagc cttcagaagg tctctgtaga tttcttccgc agcagctctc tgctcttcgg
     2761 agaatggagc ctcgaaggcc acccgctcat cggatttcga agcctggaga aaggctccca
     2821 gggtctccgg acccagcggt tcggcaaagg ctcccctgtt ccgggatgaa aggaaattaa
     2881 tgacgaaggt gtttcccttt tccgccgccc ggagatagag cccccgctcc tggggaggaa
     2941 gagcgagcag agccttcccg atgttatcgt tatcgtcggg gttgtccgcc ccgggagagc
     3001 cgaaggtaat gccgagttcc ttcgctcttt tgtccttgta ttccttcatc catttccaca
     3061 ggtctttccc cgcgggagtc ttcatgagct tatcaagggc ttccgggtcg tcgaggatag
     3121 gtttgaatat cctgtagtat ttccagcggt ttttctccca tataggcggg agaggacctc
     3181 cgatgacatg ggtcatgagg tcggagagag gtttgtaggc cgtatcaagc cgctgcttca
     3241 tgtccagcgc ccgctgggcg gctttccggg cgtcaaggag gtctccggca agtccgtatc
     3301 cttccaggat cgcttcgccc ccggagatga gcgggcctat ggtcacagac ttcccgaact
     3361 cccaggcagc gtaggctccc gcccagacag cgggaggcag aagccattcc tttccctccc
     3421 gaaggtcctt gaggtactgt tcccgggtgg agaggccgat ctgttccccc atctggatca
     3481 gcccgggaat gccgaaaaga gtcgccaccc cgtacccgat gcctttcccg accgtaagga
     3541 ggccgaaatt ctccggatcc tcccgtgcgg ctttgtcaag gtagtcggaa tatgccttga
     3601 gggcttcgta tccctggtag atcataataa tgcccgttcc gaccttcacc agcttcgacg
     3661 ggggaggggg tgtgggaacc gccggttttt tgggaagctc gggcttgatc accttcggga
     3721 ctgttttt
//
LOCUS       DAOTHD010000087         3727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_29276_length_3727_cov_3.035675, whole genome shotgun sequence.
ACCESSION   DAOTHD010000087 DAOTHD010000000
VERSION     DAOTHD010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29276_length_3727_cov_3.035675"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="P5219_02355"
     CDS             <1..568
                     /locus_tag="P5219_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX25629.1"
                     /translation="TFLGEYVLYVIKRDSRNVLLLDYEAIVQKVNPTLSERYTIDKVR
                     TKEMTDPLGNLDDYRILVAEDSPLIRKQIADVLGQGGFHNLVLTGDGREAWDRLSAPD
                     ETFDLLITDVEMPRLDGLALTRRVKEHSEFKKIPIIVFSSIMAKDIKVKAASVGAESQ
                     ITKPEIPELVDHVCRLLGRKKEKQVSAG"
     gene            565..1236
                     /locus_tag="P5219_02360"
     CDS             565..1236
                     /locus_tag="P5219_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25630.1"
                     /translation="MTSLPYWDSNVDMQIAPWFPLGVDYKPVSVNSHHSGRLSRIAAL
                     SMRLSSEFFCLTSLQSGYAGTLLRDSMFIPGKQRRIDFGFTMLFGYSAAMEEAVGALI
                     ESQGDIEGGVSFCTLWDRDIILESFKAIILVSGNPFFSEDEFLDRLGVALEKSVSTEA
                     NMPFFAEKFGYRRFFIPYETDNPVDLEISRLLYEMEFEETRKWVGPSPEVSFPPFLTL
                     DLAVD"
     gene            1357..2559
                     /gene="rpoD"
                     /locus_tag="P5219_02365"
     CDS             1357..2559
                     /gene="rpoD"
                     /locus_tag="P5219_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HRX25631.1"
                     /translation="MENDILNEKDIPAEKEDILDEKIVKKEDLSREDMAEYLGKVREL
                     ISEGKGKGFLTQKDIERHLPLENWSEEILDNVVTELQDMDIEVVSEDKEAAAVSAPVA
                     VAVDEDMLAQPTIDMEIGKLDDIPLTDPVRMYLREIGKVPLLDAAEEVELAKRVEAGD
                     ETAKKKIVDANLRLVVSIAKKYIGRGMLFLDLIQEGNLGLIRAVEKFDYRKGFKFSTY
                     ATWWIRQAITRAIADQARTIRVPVHMVETINKMVRISRQLVQRLGREPTDEEIAGEME
                     IEPSKVEEIRRIAQLPVSLETPIGEEEDSQLGDFIEDRDLPSPEEAAAGHLLHEQIEE
                     MLDALSEREREVLHFRFGLEDGRSYTLEEVGKRFGVTRERIRQIEAKALRKLRHPSRS
                     KKLKDFLD"
     gene            complement(2602..>3727)
                     /locus_tag="P5219_02370"
     CDS             complement(2602..>3727)
                     /locus_tag="P5219_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HRX25632.1"
                     /translation="VIEGVMGLFDGAGALDERGSTAHLAKILAAPVVLVVNGKAMARS
                     AAALVAGFAGFDRKVPVKAVIFNNLGSEGHYRILREAVETETGIPVLGYLPRCGSIAL
                     PERHLGLTPAAEHGSVAELTDTVRRLTEEHIDVDRLIALAASAPDLPAFRPSIFTSPP
                     AAEARIAVALDEAFHFYYRDNLDILEHLGAVLLPFSLLRDRDLPARTEGIYIGGGFPE
                     EFAAPLAGNTSIREAIRSAAEGGLPVLAECGGLMYLAERLEDRQGEVHLMAGVFPGVT
                     RMGKRLQALGYCTGRLERSVLPGGQGTALKGHLFHWSLYDTEGNDHLLSLRLEKNGTV
                     FRDGPALRNAFASYLHLHFGTNPAPAKRFLRTAARRAGRT"
BASE COUNT          767 a         1114 c         1169 g          677 t
ORIGIN      
        1 taccttcctc ggcgagtacg ttctctacgt catcaagagg gacagccgga acgtcctgct
       61 gctcgactac gaggccatcg tccagaaggt gaaccccacc ctgtcggagc ggtacaccat
      121 cgacaaggtc cgcacgaagg agatgaccga tcccctgggg aacctcgacg actacaggat
      181 tcttgtcgcc gaggattcgc ccctcatccg gaagcagatc gccgacgtgc tcggccaggg
      241 aggattccac aaccttgtgc tcacgggtga cggcagggag gcatgggaca ggctctccgc
      301 ccctgatgaa accttcgacc ttctcatcac cgacgtggag atgcccaggc tggacggcct
      361 ggccctcacc cggagggtga aggagcacag cgaattcaaa aaaattccca tcatcgtctt
      421 ttcgtccatt atggccaagg acatcaaggt caaggcggcg agtgtcggcg ccgagtccca
      481 gatcacaaag cccgagatcc ccgagctggt ggaccatgtc tgcaggcttc tcggcaggaa
      541 aaaggagaag caggtttcag caggatgaca tcccttccct actgggattc caatgtggac
      601 atgcagatcg ccccgtggtt tcctctcggg gtggattata agcctgtgtc ggtgaactcg
      661 caccacagcg gccgcctttc caggatcgcc gccctgtcca tgcgcctgag cagcgaattc
      721 ttctgtctca cgagtcttca gagcggctac gccggcaccc tgctccggga cagcatgttc
      781 atcccgggca agcagcggcg gatcgacttc ggcttcacca tgctcttcgg ctactccgca
      841 gccatggaag aggcggtggg agccctcatc gaaagccagg gggacatcga gggaggagtc
      901 tccttctgca ccctctggga cagggacatc atcctcgaga gcttcaaggc catcatcctg
      961 gtcagcggaa atcccttctt cagtgaggac gaattcctgg acaggctcgg cgtcgccctg
     1021 gaaaaaagcg tgtcgaccga ggcgaacatg cctttcttcg ccgagaagtt cggataccgg
     1081 aggttcttta ttccctacga gaccgacaac cccgtagacc tggagatcag ccgtcttctc
     1141 tacgagatgg agttcgagga aacgaggaaa tgggtggggc cttcgccgga agtctccttt
     1201 ccgccgttcc ttacccttga tcttgcagtt gactgaagta tggtataata tttgtccgct
     1261 caaaaagaaa ggcggaggag aactctgaaa cggccccgat tcccggaagg aagtctggac
     1321 cgttcttgcc gtagtgaaaa ggggactggt tgctcgatgg agaatgatat tctgaacgag
     1381 aaggatattc ctgcggagaa ggaagatatc ctggacgaga agatcgtcaa gaaggaggat
     1441 ctctcgcggg aagacatggc cgagtacctg ggaaaggtgc gggagctcat ctccgaagga
     1501 aagggcaagg gcttcctcac ccagaaagat attgagcggc atctccctct cgagaactgg
     1561 agtgaagaga ttctcgacaa cgtggtgacc gagctccagg acatggatat agaagtggtg
     1621 agcgaggaca aggaggccgc ggctgtgtcc gctcctgtcg ccgtggcggt ggacgaggac
     1681 atgcttgccc agccgaccat cgacatggag atcgggaagc tcgacgacat cccccttacc
     1741 gaccctgtgc gcatgtatct ccgggagatc ggcaaagtcc cccttctcga cgccgctgag
     1801 gaggttgaac tggccaagcg ggtggaggcc ggcgacgaga cggcgaagaa gaagatcgtg
     1861 gatgccaatc tccgtctcgt ggtgagcata gcgaagaaat acatcggccg ggggatgctc
     1921 tttctcgacc tcatccagga gggaaacctg gggcttatcc gggcggtgga gaagttcgac
     1981 taccggaagg gattcaagtt cagcacctac gccacctggt ggatccggca ggccatcacc
     2041 agggccatcg ccgatcaggc caggacgatc cgcgttcccg tccacatggt ggagaccatc
     2101 aacaagatgg tccgcatctc ccggcagctg gtccagcgcc tgggccggga gcccaccgac
     2161 gaggagatcg ccggtgaaat ggagatcgag ccgtccaagg tggaggagat ccgccgcatc
     2221 gcccagcttc cggtttccct ggagactcct ataggcgagg aggaggacag ccagctcggc
     2281 gacttcatcg aggaccggga tcttcccagc ccggaggagg cagccgcagg acacctgctc
     2341 cacgagcaga tcgaggagat gctggacgcc ctctccgagc gggagaggga agtgctccac
     2401 ttccgcttcg gtctcgagga cggccggtcc tataccctcg aggaagtggg aaagcggttc
     2461 ggcgtcacca gggagcgcat ccggcagatc gaggccaagg ctctccggaa gctccgccat
     2521 cccagcagga gcaaaaaact caaggatttc ctcgattagc gaaaaaaacg aagcccccgg
     2581 atttcggtcc gggggttttt gttatgttct ccctgctctt cgggcggctg tccgaaggaa
     2641 tcgcttcgcc ggtgccgggt tcgttccgaa gtggaggtgg aggtaggagg cgaaggcatt
     2701 ccggagtgcc gggccgtccc ggaagaccgt gccgtttttc tcgaggcgca gggagagaag
     2761 atgatcgttg ccctcggtgt cgtacagtga ccagtggaag aggtgcccct tcagcgccgt
     2821 gccctgcccc ccgggaagga cggaccgttc cagcctgccg gtgcagtagc ccagggcctg
     2881 gagccttttc cccatcctcg tgacgccggg gaacacccct gccatgaggt ggacctctcc
     2941 ctgccggtcc tcgagccgtt ccgcgaggta catcagcccg ccgcactccg ccaggacggg
     3001 aagccccccc tccgcggccg accgtatggc ttcccggatg gaggtgtttc ctgccagcgg
     3061 ggcagcgaat tcctcgggaa agcctccccc gatgtagatc ccctccgtcc gtgccggcag
     3121 atccctgtcc cgcaggaggc tgaagggaag gagtaccgct cccaaatgtt ccaggatgtc
     3181 caggttatcc cggtagtaga aatgaaaagc ctcgtccaga gccacggcga tcctcgcctc
     3241 cgccgccggg ggggaagtaa agattgaagg cctgaaggcg ggaaggtccg gcgccgatgc
     3301 cgccagggcg atcaggcggt caacgtctat gtgctcctca gtcagtctgc ggacggtatc
     3361 cgtgagttcg gcgaccgatc cgtgctctgc cgccggggtg agcccgaggt gccgctcggg
     3421 aagggcgatg gatccgcacc ggggaaggta gccgaggacg gggatgcccg tttccgtctc
     3481 caccgcttcc ctgaggatgc ggtaatgtcc ctcgctgccc aggttgttga agatcacggc
     3541 cttcacgggc actttccggt cgaacccggc gaatcccgcc accagggcgg cagcgctgcg
     3601 ggccatggcc tttccgttca ccacaagcac tacaggggcg gcgaggatct ttgccaggtg
     3661 ggcggtgctt ccccgttcgt ccagggctcc cgctccgtcg aaaaggccca tcacgccctc
     3721 gatgacg
//
LOCUS       DAOTHD010000088         3715 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_29406_length_3715_cov_3.068033, whole genome shotgun sequence.
ACCESSION   DAOTHD010000088 DAOTHD010000000
VERSION     DAOTHD010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29406_length_3715_cov_3.068033"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..994
                     /locus_tag="P5219_02375"
     CDS             59..994
                     /locus_tag="P5219_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25633.1"
                     /translation="MNGKTLFAVALACILVLPVPSSGSPDGTGPVPIVFSRGDGLFLT
                     DTEGNSPVLLRKGYDPEISPDGTEVAFTVSTGKDGSGRQIGLYTIATTKSRNYKSVIP
                     GENSYGPRWSPDGTMIVFNHWDPDQSDWVLGLLTLTDGTFRVLAPELKGVYSPFWSAD
                     SSSVYCHDLENFFRVSVETGRTAALRRFSEILGEAMPTSAVRFSVSPDGTRWLFDGEV
                     KDTSDWLKSGDGLISALFLHTPADGTTRRITDDRICAIGPAWLPGGENFLFYGYTEKE
                     NSGEGSGFAVFRGSLAKGKITLLVPDGGTPSARLR"
     gene            complement(1005..1511)
                     /locus_tag="P5219_02380"
     CDS             complement(1005..1511)
                     /locus_tag="P5219_02380"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HRX25634.1"
                     /translation="MSEQRRLNRLKDAAGLAGWIALCFAAAAVGGAATAGAPGFFGEL
                     SRPSWAPPAWIFGPVWTVLYLMMGLSAWTAWRERGFREAPAAFGLFLLQLGANALWSW
                     IFFFWRQGALAFGEILLLWILLAATMQQFRRIRPAAALLLVPYLAWTTFAAVLAFSIW
                     RLNPAVLG"
     gene            complement(1504..2061)
                     /locus_tag="P5219_02385"
     CDS             complement(1504..2061)
                     /locus_tag="P5219_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HRX25635.1"
                     /translation="MDMNDFPFPEELKSLVDFHGHFCPGVLIGWRASKLALKLLGMSR
                     DRDEEMVAVAENDACGVDAVQFILGCTFGKGNLHFRDYGKQAFTVFRRSDGRGVRLVL
                     NAPERELTREESAARLLEEPDDVLFTVGEPKEPMPERAVIRKSGVCALCGERVMETRL
                     MPLPDGRRVCIPCSGGTPEGGGNNE"
     gene            2408..3064
                     /locus_tag="P5219_02390"
     CDS             2408..3064
                     /locus_tag="P5219_02390"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HRX25636.1"
                     /translation="MEHSVRSGESVAFIAGRYGVSPRTIERANELAVREDSVLAGEKL
                     LVPRKDSDLLPTLAEYRARQRGETLVPLIRIEEPLPPPPPPKKAEAILADGIEPLIRP
                     LEGRVSSPFGPRGGRLHDGIDIPARAGTPILAVRSGRVIFSGVIRGFGNTVTIDHGNG
                     FVTRYSHNCANLVKKGAWVRRGQPVAKVGKTGRTTCNHLHFSVLIKGKPVNPEKFLPK
                     "
     gene            complement(3149..>3715)
                     /locus_tag="P5219_02395"
     CDS             complement(3149..>3715)
                     /locus_tag="P5219_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HRX25637.1"
                     /translation="LWQSLFRPGTEPEKGAANALSLLSLLVKDGDPARWDSAAGFLYP
                     SETPKPLAAADAVYMAALYLLRMEEDMAGHLAASLFEGLLQSSRAKHFFLTTGPAEYG
                     VLVSEMTARGLLPSFGKWPESAQAGRLPFAAPVRGWVTHGRALSEDMVFLDGAGRPQS
                     NGTYAWDRKRGRIYTVVEERDPFFIPWD"
BASE COUNT          774 a         1163 c         1045 g          733 t
ORIGIN      
        1 ctttcctgta tcatggaatg acaggattcc ggaaagaacc ctgggaggag gtctgtccat
       61 gaacggaaaa acgctttttg ccgttgcgct cgcctgcatt ctggttctgc ccgtgccgtc
      121 ctccggctcg ccggatggaa ccggtcccgt tcccattgtg ttttcaaggg gtgacgggct
      181 tttcctcacc gacacggaag gaaacagccc ggttcttctc cggaaaggat acgaccccga
      241 aatctccccc gacggaacgg aagtggcctt caccgtctcg acgggaaaag acgggagcgg
      301 gagacagatc ggcctgtaca ccatcgccac tacaaaatcg agaaactaca agtccgtcat
      361 ccccggggaa aacagctacg gcccgaggtg gtcccccgac ggaaccatga tcgtcttcaa
      421 ccactgggac ccggaccagt cagactgggt actgggtctc ctcaccctca ccgacggaac
      481 cttccgggtg ctcgccccgg agctgaaggg ggtctattct cccttctggt ccgccgacag
      541 ctcttcagtc tactgccacg acctggaaaa cttcttccgc gtctcggtgg agacgggacg
      601 aacggcggct ctccgaaggt tttccgagat tctcggcgaa gccatgccca ccagcgccgt
      661 ccgattctcc gtttcccccg acggaacccg ctggctcttc gacggtgaag tgaaggatac
      721 gtccgactgg ctgaaaagcg gcgacggcct catctccgcc cttttcctcc atacccctgc
      781 ggacggaaca acccgccgga taaccgatga ccggatatgt gccatcggcc cggcatggct
      841 gccgggaggg gaaaacttcc tgttctacgg gtacacggag aaggaaaaca gcggagaggg
      901 atcgggtttt gccgtctttc ggggttccct ggcgaaagga aagataaccc tgcttgtgcc
      961 ggacggagga actccctccg cccggctccg gtaacaggag aggcttaccc gaggaccgcc
     1021 gggttcagcc tccatatgga aaaagccagg acggcggcga aggtggtcca ggccagatag
     1081 gggaccagca gcagcgctgc cgccgggcgt attctgcgaa attgctgcat ggttgccgca
     1141 aggagaatcc agagcagaag aatctcccca aaggcaaggg ctccctgccg ccagaaaaag
     1201 aaaatccagc tccagagagc gtttgcgccg agctggagca aaaagaggcc gaaagcggcg
     1261 ggagcttcgc ggaaccccct ctcccgccag gccgtccatg cggagagccc catcatcagg
     1321 tacaggaccg tccatacggg gccgaaaatc catgcaggag gggcccatga gggcctggac
     1381 agctccccga aaaaaccggg ggcccctgcc gtcgccgccc caccaactgc cgcggcggca
     1441 aaacagaggg caatccagcc cgcgaggccg gcagcgtcct tcaaacggtt cagtcttcgt
     1501 tgttcactca ttgttcccgc ctccctccgg agtacccccg gaacagggaa tgcacactct
     1561 ccggccgtcc gggagcggca tgagccgagt ctccatgact ctttcgccgc agagggcgca
     1621 gacgccggat tttctgatca cggccctttc gggcatgggt tccttcggct cccccacggt
     1681 gaacagaaca tcgtcgggtt cctccagaag gcgcgccgca gactcctccc tcgtgagctc
     1741 ccgttcgggg gcgttgagga caagccggac ccccctgccg tcggaccgtc tgaaaacggt
     1801 gaaggcctgc ttgccgtaat cccggaaatg gaggtttccc ttgccgaagg tgcacccgag
     1861 gatgaactgc accgcatcca cgccgcaggc atcgttttcg gccaccgcca ccatctcctc
     1921 gtccctgtcg cgggacatgc cgagaagctt cagtgcaagc ttcgacgctc tccatcctat
     1981 gagcacgccg ggacagaaat gcccgtgaaa atccaccagg gatttcagtt cttcaggaaa
     2041 gggaaaatcg ttcatatcca ttcttggtct ccttgaattc cttcggggca aaatgccacg
     2101 atttattaat ttcgtagcat tattgtagca ttttttctcc cccatcactt gggatttctc
     2161 attttcacag ttttttgccc tttttttctt cgaaaaggag aaaaataaaa atcagtattt
     2221 ttactgagaa gaacccctcc ccactccgat acgataacct gaccaccggt aaagcatcct
     2281 tatcgaaggg ggcgattctg tgcctttttc ccggcgtttc gctttcttgt ccgttctttc
     2341 aggctttttt tccgttcttg cagttctttt cttttctccg gccgcttcgg ccacttccct
     2401 tccgtgggtg gaacattccg ttcgcagcgg tgaatccgtg gcattcatcg cggggagata
     2461 tggcgtatcc ccccggacca tcgaacgggc caacgaactg gcagtccgtg aggacagcgt
     2521 cctcgccggc gagaagcttc tcgttccgag gaaggattcg gacctcctgc ccaccctggc
     2581 ggagtacagg gcccggcagc ggggtgaaac ccttgttcct ctgatccgca tcgaggaacc
     2641 ccttcctcct cctcctccgc ccaaaaaagc ggaagcgatc cttgcagacg gaatcgaacc
     2701 ccttatcagg cccctcgagg ggcgggtttc ctcccccttc ggaccaagag gaggaagact
     2761 ccatgacggc atcgacatac ccgcacgggc aggaactccc atcctggcag tccggtcggg
     2821 aagggtcatt ttttccggtg tcatccgcgg tttcggaaac acagtcacca tagaccacgg
     2881 caacgggttc gtcacccggt acagccacaa ctgtgcgaac ctggtgaaaa agggagcctg
     2941 ggtccggcgg ggacagcctg tcgccaaggt gggaaaaacc gggcggacaa cctgcaatca
     3001 cctccatttc agcgtcctga tcaagggaaa gcctgtaaac cccgagaaat tcctgccgaa
     3061 atagctgaag cggcaggcga gtcctgacat tttttcttcg attttcctca acaaaaaaac
     3121 cccggatcgt tccggggttt ttcctgtttc agtcccaggg aatgaagaac ggatcccttt
     3181 cctccactac tgtgtaaatt ctgcccctct tccggtccca ggcgtatgtg ccgttcgact
     3241 gaggcctgcc ggccccgtcg agaaaaacca tatcctccga aagagccctg ccgtgggtca
     3301 cccagccccg gaccggggcg gcgaaaggca gcctgcccgc ctgcgcgctt tccggccatt
     3361 ttccgaagga ggggagaagt ccccgggcgg tcatttcaga taccaggacg ccatattccg
     3421 ccggtcccgt ggtgagaaag aagtgctttg cccgggatga ctggagaagc ccttcgaaga
     3481 gcgaggccgc caggtggcct gccatatcct cctccatcct gaggagatac agggctgcca
     3541 tgtagaccgc atcagctgcg gcaaggggtt tcggcgtctc tgaagggtag aggaagccgg
     3601 cggccgagtc ccaccgggca gggtcaccgt ccttcacaag gagggagaga agggagaggg
     3661 cgttcgccgc tcctttctcg ggctccgtcc cgggacggaa aagtgactgc cagag
//
LOCUS       DAOTHD010000089         3715 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_29409_length_3715_cov_2.936885, whole genome shotgun sequence.
ACCESSION   DAOTHD010000089 DAOTHD010000000
VERSION     DAOTHD010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29409_length_3715_cov_2.936885"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="P5219_02400"
     CDS             <1..288
                     /locus_tag="P5219_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HRX25638.1"
                     /translation="RQEILDGVNELIAEGRSRSLTEEDLDRKMYLPDLPPPDLIIRTS
                     GEQRLSNFWLWQCVYSELYFTDVLWPDFGPGDLEKAIGQFTGRERRYGAVK"
     gene            272..1096
                     /locus_tag="P5219_02405"
     CDS             272..1096
                     /locus_tag="P5219_02405"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRX25639.1"
                     /translation="MAQSNEALKELMVRGGAGAAVVAVVLGSLYVGGMTWTVLVSVLA
                     LVSLGEFYSMLSRKFKISRGVGFLAGGLILFTASDGIHPISLVLTLSITAFVILFVEI
                     LRRQIKGESFAIWNMGGTLGGILYIIIPWTFMILLRRLPSGAILLFSMFLCTWCCDVS
                     AYLIGSKWGRIRLCENVSPKKTWEGFLGGLSAALLAGVSVAFVLETPPPPFLFVGLIC
                     GVAGQIGDLAESVIKRETGVKDSGSVIPGHGGILDRFDSVLVSGLLVYVLFGVILR"
     gene            1093..2277
                     /gene="dxr"
                     /locus_tag="P5219_02410"
     CDS             1093..2277
                     /gene="dxr"
                     /locus_tag="P5219_02410"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRX25640.1"
                     /translation="MKRTRLFVIGCTGSVGGSVLSVCRTFPDYFEVRALAAHSSSAET
                     AKLAGEFQADRVVLSDPAAAADLRRSCPGGLKVLEGAEALEETASSPDIDHVVVASSG
                     TGAIGALMAALRSGKDVSLANKESIVAAGQWILPLIGRKGQLRPLDSEHNAVWQCMGD
                     RDPASVSRIILTASGGPFRTFSREDLETVTPSMAVRHPVWSMGAKISVDSATLMNKGI
                     EILEAMALFSLPAERVDAVICPDSFVHGIVEFRDGSFLLSASSPDMRLPCASALFFPE
                     KSPFPPVPVPSLQGRTVSFLQPDEQRFPSLRLAKEAARKGGAFPALLVGADEVAVDRF
                     LAGEIGFTGIPSVVESVLEQWNGPVPASLADALDILDEGRRLAAAVCRRLKSSRPARR
                     SI"
     gene            2278..3312
                     /gene="rseP"
                     /locus_tag="P5219_02415"
     CDS             2278..3312
                     /gene="rseP"
                     /locus_tag="P5219_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRX25641.1"
                     /translation="MASLLSFLLVIGICVMVHEYGHYITARFFDVQVHEFAFGMGPVV
                     FQRKGKHTLWSVRAFPVGGFVRLAGMEEENEDETVEPGRAFYDKAAWKRFLILVNGSV
                     ANVLLALLLTAFFLSGHGVVNLEETVIGEVMEGYPAQAAGIVPGDRVLSVNGTSVETW
                     RAMSAEIRQEAPKGPVLFSVRRGEEILSISAVILPDREQGIPLFGIRPGMKRFPLGEA
                     FSSALSYTVNMSVEMIRGIVRWISGTEKVDVTGPVGIASMAGEAARKGLWTFLSFLAL
                     INLNLGLINLLPFPALDGGRLFFTAGEMLLRRRLPPKIENYIHMTGFFLLISLILYIT
                     WQDVVRLFRG"
     gene            3317..>3715
                     /locus_tag="P5219_02420"
     CDS             3317..>3715
                     /locus_tag="P5219_02420"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRX25642.1"
                     /translation="MAETVIIGGLSIGNGHPVRVESMLKIPLSSREECLEQCRSLASC
                     GCELVRAAFPSPELAGDLAWLNDKSPVPLMADIHFDPALALAALEAGTPSIRINPGNM
                     ARARLREVVASAREKKAVIRIGANGGSLNSA"
BASE COUNT          681 a         1052 c         1123 g          859 t
ORIGIN      
        1 cggcaggaaa tccttgacgg agtgaatgag ctgattgcgg aaggcaggag caggtcgctc
       61 acggaggaag acctggacag gaagatgtat cttcccgacc ttcctccgcc ggatcttatc
      121 atccggacga gcggagagca gcggttgagc aatttctggc tttggcagtg cgtctacagc
      181 gaactgtatt ttaccgatgt cctttggccc gacttcggac cgggcgatct cgaaaaggcc
      241 ataggacaat tcaccggcag ggagagacga tatggcgcag tcaaatgaag cattgaaaga
      301 acttatggtc aggggaggtg ccggggctgc cgtcgtggct gtcgtgctgg ggagtctcta
      361 tgtgggggga atgacctgga cggtcctggt ctccgttctt gcccttgttt ccctcggaga
      421 gttctacagc atgctttcaa ggaaattcaa gatttcccgg ggagtcggct ttcttgccgg
      481 cgggctcatt ctcttcacgg cttccgacgg catccacccc atttccctgg tgctcaccct
      541 gagcattacg gccttcgtca tccttttcgt ggaaatactg cggaggcaga tcaaggggga
      601 gagctttgcc atctggaata tgggaggcac cctggggggc atcctgtaca tcatcatccc
      661 atggaccttc atgatcctgc tccggcggct tccctccgga gccatcctcc ttttctccat
      721 gtttctctgc acatggtgct gtgatgtgtc ggcatacctt atcggttcga agtgggggag
      781 gatccggctc tgtgaaaacg tcagcccgaa aaaaacctgg gaaggatttc tcggggggct
      841 ttccgcggct cttctcgcgg gtgtgtccgt ggcgttcgtt ctggagacgc ctcctcctcc
      901 ctttcttttc gtcggcctca tctgcggcgt ggccggccag ataggcgatc ttgcggaatc
      961 agtcatcaag cgggaaacag gggtaaagga cagcggaagc gtcatccccg gccacggcgg
     1021 aatactggac cgtttcgaca gcgttcttgt cagcggcctc cttgtatacg ttcttttcgg
     1081 ggtcattctc agatgaagag aacccggctt tttgtcatcg gatgtaccgg gagcgtcggc
     1141 ggctccgtcc tttccgtatg ccgcactttc cctgattatt ttgaagttcg tgccctggcg
     1201 gctcattctt cctccgcgga aaccgcaaag cttgcggggg aattccaggc tgaccgggtg
     1261 gtgctttccg atcccgcagc ggcagcggat ctcagaagat cctgtcccgg cgggctgaag
     1321 gtcctcgaag gcgcagaagc tcttgaggag accgcctcga gtcccgacat cgaccacgtg
     1381 gtcgtggcgt cctcggggac aggagccatc ggcgccctta tggcagccct gaggagcggc
     1441 aaggatgttt cacttgccaa caaggagagc atcgtggccg cagggcagtg gattctcccg
     1501 ctgatcggcc ggaaagggca gctcaggccc cttgacagcg aacataacgc cgtgtggcaa
     1561 tgcatgggag acagagatcc ggcttcggtt tcccggatca tccttacggc atcgggggga
     1621 cctttccgca ctttttccag ggaagatctg gagacagtga ctccctccat ggcggtacgc
     1681 cacccggtat ggagcatggg tgccaaaatc agcgtggaca gcgccacgct gatgaacaag
     1741 gggatcgaga tcctcgaggc catggcgctc ttctcccttc ccgccgagag ggtcgatgca
     1801 gtcatatgcc ctgattcctt cgtccacggc atcgtggagt tcagagacgg atcttttctt
     1861 ctctccgcct cttctcccga catgcgcctc ccctgtgcct cggccctgtt ttttcccgaa
     1921 aaaagcccct ttccccccgt gccggtcccc tctttgcagg ggaggacagt ttccttcctt
     1981 caaccggacg aacagcgctt tccgtccctt cgcctagcga aggaagccgc ccgcaaggga
     2041 ggggctttcc ccgctctgct ggtcggggct gacgaggtgg ccgtggaccg ttttctggcc
     2101 ggtgaaatcg ggtttaccgg gattccgtcg gtggtggaat cggttcttga acaatggaat
     2161 ggaccggtac ctgcttctct cgccgatgcc ctggacattc tggacgaagg aagaaggctg
     2221 gccgcggcgg tgtgccggcg gctgaaatct tcccgtcccg caaggagatc gatctgaatg
     2281 gcgagtctgc tgtcgtttct tttagtcata ggtatttgcg tcatggttca tgaatacgga
     2341 cactacatca cggcacggtt ttttgatgtc caggtgcacg agttcgcctt cggcatgggg
     2401 ccggtagttt tccagcgaaa ggggaaacat accctgtggt cggtcagggc tttccccgtg
     2461 ggaggctttg tccgcctggc aggaatggag gaagagaacg aggatgagac tgtcgaaccg
     2521 ggcagggcct tctacgacaa ggctgcctgg aagcgttttc ttatcctggt gaacggctcc
     2581 gtcgccaatg tcctgcttgc ccttctcctg acggcgttct ttctttcggg acacggcgtt
     2641 gtcaacctgg aggaaaccgt catcggggaa gtcatggaag ggtacccggc tcaggcggcg
     2701 ggtatcgtgc cgggagacag ggttctttcc gtgaacggca cctctgtgga aacctggcgg
     2761 gccatgtcag cggaaatccg tcaggaagcc ccgaaaggac cagttctttt ttccgtccgc
     2821 aggggagagg agatcctttc catatcggcg gttatacttc ctgacaggga acaggggatt
     2881 ccccttttcg gaatccgccc cggcatgaaa cggttccccc taggggaggc tttttcatct
     2941 gccctttcgt acacggtgaa tatgagcgta gagatgatca ggggcatagt gcggtggatt
     3001 tcgggtacag agaaagtgga cgtcaccggg ccggtgggca ttgcctccat ggcgggtgaa
     3061 gcggccagga aggggctgtg gacgtttctt tccttcctgg cgctcatcaa cctcaacctg
     3121 ggactgatca accttcttcc tttccctgcc ctggatgggg ggcggctctt cttcaccgca
     3181 ggggaaatgc tcctcaggag gagacttccg ccgaagatcg agaattacat ccacatgaca
     3241 ggctttttcc tcctgatctc gctcatactc tatatcacgt ggcaggatgt cgtccgcctg
     3301 ttcagggggt aggattttgg cagaaacggt catcattggc ggcctttcca tcggaaacgg
     3361 ccatcccgtg cgggttgaga gcatgctcaa aatacccctc tcctcaaggg aagagtgtct
     3421 cgaacagtgc cgctcccttg cctcctgcgg atgtgagctg gtccgggcag cctttccttc
     3481 ccctgaactc gccggggatc ttgcatggct gaacgataaa tcacccgttc ccctcatggc
     3541 ggacattcat tttgatccgg ccctcgccct ggcggccctg gaagcgggaa cgccgtccat
     3601 ccggatcaac ccggggaata tggcccgggc ccgcctccgg gaggttgtcg cttcggcacg
     3661 ggagaaaaag gcggtcatac ggataggtgc caacggcgga tctctcaatt ccgcc
//
LOCUS       DAOTHD010000090         3705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_29506_length_3705_cov_2.999726, whole genome shotgun sequence.
ACCESSION   DAOTHD010000090 DAOTHD010000000
VERSION     DAOTHD010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29506_length_3705_cov_2.999726"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..1110)
                     /locus_tag="P5219_02425"
     CDS             complement(184..1110)
                     /locus_tag="P5219_02425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRX25643.1"
                     /translation="MTAHKEADDLLRKNEEELQALLNAIPAVLLVVAEDGECLRVTGS
                     RDTPLVKEAAGLVGKKVAEALPPERGSEILVPVKRCIETGTPQFFTFEISLAGRTYFQ
                     EARVSPFEGSFGGKRAAIVVALDATSRKELEDEIMLQAGHDPLGSPSNRGHFNRKLLL
                     SLAEAQRTRSSLAFFMIDLDHFNAVNEAIGRDMADLLLKGVARKIQGACRQDDILYRM
                     EGVNFYLILPRLRNREEAAMTADRILGAIRTAAAGYSGDITLSATIGISLFPENGQDG
                     KSLLRTAEAALSSGKAAGGDRFIFAEPAPAEE"
     gene            complement(1300..1449)
                     /locus_tag="P5219_02430"
     CDS             complement(1300..1449)
                     /locus_tag="P5219_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25644.1"
                     /translation="MESCGGVNFEHVVNISPVVSFVWISGGGDVPCFVSGAVASFGYA
                     PSDFT"
     gene            1625..2299
                     /locus_tag="P5219_02435"
     CDS             1625..2299
                     /locus_tag="P5219_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRX25645.1"
                     /translation="MVYHLKKDNLASGVYERIKKGILSLEFPPEFILQERNLAEELGV
                     SRTPVREAVHRLSQEGWLRVNARRNIQVRPVSVSDLREVFQARRILERDALELLLSTG
                     LAREAGRKMAAMAAVMNDSRGSLFSFITADQSFHSVLFLVLGNSVLRKFWNAVSEEMI
                     WLGMQAMNEKRYDDVLEEHGKILGAVEEGKKRAAREALLDHLEKTEDILLRKIDDVSS
                     SYGRGS"
     gene            2304..2987
                     /locus_tag="P5219_02440"
     CDS             2304..2987
                     /locus_tag="P5219_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2848 family protein"
                     /protein_id="HRX25646.1"
                     /translation="MRMKSAVLGKDGVSGEIILSWDACVAAGYTGRDQAGVLAHVEEL
                     RKIGVPAPEKVPSMYWVEPERVSNTGLLWVVGESTSGEGEVFLARDEGNNLCVTVASD
                     HTDRALETVSVAKAKQACSKVIGSFFWRVDEVRGHWDSIELRTWNDGKLYQEGTLGKM
                     LPPEKLFELAREDAPAGTGKLALFSGTLPVLGEGIVYGTHYVLSLKDPVLGREIRHEY
                     SVRVLPDRN"
     gene            3000..>3705
                     /locus_tag="P5219_02445"
     CDS             3000..>3705
                     /locus_tag="P5219_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HRX25647.1"
                     /translation="MKLRVGVLQLDVALGDRERNRKNVEKWMASVSVPSDIPTAVVIP
                     EIWDVGYALDRAAELADPEGRGAAEFLGGLARKYGVWFVGGSVLASTGSGYANRAQVI
                     NPRGELVAYYDKVHLIRLMEEEKYFTGGRKECLFSLEGAKAGCVICYDIRFCEWLRTY
                     ALQGAEVLFVSAEWPSPRADHWKTLLRARAIENQMYVVACNRCGTTGDTHFGGGSMIL
                     GPKGEILFEGGEGEEAG"
BASE COUNT          724 a         1053 c         1220 g          708 t
ORIGIN      
        1 gaagagcgcc gatggggcag catggacgca ggtgacgcct gcggctgcct ggtcagaacg
       61 gggcccacgg ggaccaacgt gaacgacctt gcggtgctgc tcgtcagata gcccccggcg
      121 gacaaagaag aagggctccc cttttcgggg agcccttttg tcatcaacag tgggaaaggg
      181 cagctattct tctgccggtg cgggttccgc aaagatgaag cggtctcccc ccgcggcctt
      241 tcccgatgag agggcagctt cagccgtcct gagaagggac tttccgtcct ggccgttttc
      301 cggaaagagg ctgatgccga tggtggccga cagggtaatg tccccgctgt atcccgccgc
      361 ggccgtgcgg atggccccga gaatcctgtc cgccgtcatg gcggcctcct ctctgttccg
      421 cagcctgggg agaatgaggt agaagttcac tccctccatg cggtagagga tgtcgtcctg
      481 gcggcatgct ccctggatct tccgcgccac tcccttgagc aggagatcgg ccatgtcccg
      541 tccgatggcc tcgttcaccg cgttgaaatg gtccaggtcg atcatgaaaa aggcaaggga
      601 cgacctggta cgttgggctt ccgccaggga aaggagcagc ttccggttga aatgccccct
      661 gttggacggg ctcccgaggg gatcgtggcc cgcctggagc atgatttcat cctccagttc
      721 ctttcttgag gtagcgtcga gagcgactac gatggccgcc cgcttgcccc cgaaagagcc
      781 ttcgaagggc gatacccggg cttcctgaaa ataggttctg ccggcgaggg aaatttcgaa
      841 ggtgaaaaat tgcggggttc cggtttcaat gcaccgtttt accggaacga ggatttcgct
      901 gccccgttcc ggcggcagcg cctcggccac tttctttccc acgagtccgg cagcttcctt
      961 caccagggga gtgtcccggg atcctgtgac ccgaaggcat tccccgtctt ccgccacgac
     1021 gaggagcacg gcgggaatgg cgttcagcag ggcctggagc tcttcctcat tcttcctgag
     1081 aagatcgtcc gcttccttgt gggccgtgat gtccgcaagg gtccagagaa attcgccggg
     1141 aacgccggga gtgtgctcca ggcggcagat cctgtgggaa acccaccgcc agtttccgcc
     1201 ggcatcggca atcctgtaga cccgtgaggc gcagtccggt gtgaagtcga gtccctccct
     1261 gtcggcaggg tgaacaatat cggcatagcg gattttcccc taggtaaaat cggaaggagc
     1321 atatccgaaa gaagcgaccg ctcccgatac gaagcacggc acgtccccgc cgccggaaat
     1381 ccagacaaaa gaaaccaccg ggctgatgtt gactacgtgt tcgaaattga ctccgccgca
     1441 actttccata tccgattcag ctcccctttt atttctgttc ttcaaaggcc ggaaaggcgc
     1501 cccacgccgc ttgccagcaa ttgtaccata cagaaagtcc cggggttcgg agggattttg
     1561 catgccatat cctcttgaca ggatgtattt gattcactat tcttgtggta taccatggga
     1621 ggaagtggta taccacttga aaaaggacaa tctcgcatca ggagtctatg aaagaataaa
     1681 aaagggaatt ctttctctgg agtttcctcc ggagttcatc cttcaggagc ggaacctcgc
     1741 agaggagctc ggcgtgagcc ggacacccgt ccgggaggcg gtccaccgcc tctcccagga
     1801 ggggtggctc cgggtgaatg cccggaggaa catccaggtc cggccggttt ccgtatcgga
     1861 tctccgggag gtgttccagg cccggcggat cctggagcgg gacgccctgg agcttctcct
     1921 ttccacgggt ctcgcccggg aggcgggacg gaagatggcc gccatggcgg cagtgatgaa
     1981 cgattcccgg ggaagcctgt tctccttcat caccgcggac cagtcttttc attcggtgct
     2041 cttcctcgtc ctcgggaatt ccgttttgcg gaaattctgg aacgccgtga gcgaggagat
     2101 gatctggctg gggatgcagg ccatgaatga aaaacggtac gacgacgtcc tcgaggaaca
     2161 cggaaaaatc ctcggggccg tggaagaggg caaaaaacgg gctgcccggg aggcccttct
     2221 ggaccatctc gaaaaaacag aggacatact gctgcggaag atcgacgacg tatcgtcctc
     2281 atacggaaga gggagctgac gacatgcgga tgaaaagcgc ggtactgggg aaggacggtg
     2341 tctccgggga gatcattctc tcctgggatg cctgcgtggc cgccggatat accggccggg
     2401 accaggcagg ggtgctcgcc cacgtggagg agctgcggaa gataggggtt cccgccccgg
     2461 aaaaggtgcc ctccatgtac tgggtggagc ccgagcgggt gtcgaacacc gggctgctct
     2521 gggtggtggg ggaatccacg tcgggcgaag gagaggtgtt cctcgcccgg gacgagggaa
     2581 acaatctctg cgtcaccgtg gccagcgacc atacggacag ggctctcgag acggtgtcgg
     2641 tggccaaggc gaagcaggcc tgctccaagg tcatcggctc cttcttctgg agggtggacg
     2701 aggtcagggg gcactgggat tccatcgaac tgagaacctg gaatgacggg aaactctacc
     2761 aggaggggac cctcgggaaa atgcttccgc cggagaagct cttcgagctg gcccgggagg
     2821 acgccccggc ggggacggga aagctcgccc tgttcagcgg caccctgccg gtgctcggcg
     2881 aggggatcgt ctacgggacg cactatgttc tctccctgaa ggatcccgtt cttggaaggg
     2941 agatccggca cgagtattcc gtgcgggtcc ttcccgaccg aaactgagga ggtggttccg
     3001 tgaagcttcg cgtcggcgtg ctgcagcttg atgtcgccct gggcgaccgt gaaaggaaca
     3061 ggaaaaacgt ggagaagtgg atggcctccg tgtccgttcc gtccgacatt cccacggcgg
     3121 tggtcatacc ggaaatctgg gacgttgggt atgccctcga cagggcggcg gagcttgccg
     3181 accccgaggg caggggggcg gcggaatttc tcgggggtct ggccaggaag tatggcgtct
     3241 ggttcgtcgg cggttccgtg ctggcctcca cggggagcgg atacgcaaac cgggcccagg
     3301 tgatcaaccc gaggggcgag ctggtggcct actacgacaa ggtccatctc atccgtctca
     3361 tggaggagga gaagtatttc accggcggaa ggaaggagtg cctcttctcc ctcgaaggag
     3421 cgaaggcggg ctgtgtcatc tgctacgaca tccggttctg cgagtggctc cgcacctacg
     3481 ccctccaggg cgcggaggtg cttttcgtga gcgccgaatg gcccagtccc cgggcggatc
     3541 actggaagac cctgctccgg gcgcgggcaa tagagaacca gatgtacgtg gtggcctgca
     3601 accggtgcgg caccacggga gacacccatt tcggcggcgg ttccatgatc ctcggcccca
     3661 agggagagat tctcttcgag ggcggggaag gcgaggaagc agggt
//
LOCUS       DAOTHD010000091         3705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_29509_length_3705_cov_2.837808, whole genome shotgun sequence.
ACCESSION   DAOTHD010000091 DAOTHD010000000
VERSION     DAOTHD010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29509_length_3705_cov_2.837808"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..501
                     /locus_tag="P5219_02450"
     CDS             82..501
                     /locus_tag="P5219_02450"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HRX25648.1"
                     /translation="MTVQWSDDLAIGIGIIDDQHKKLVERIASFSKAVESGDRNRMED
                     TVNYLIGYAIQHFGAEELIMIRNCYDQFREHRDEHSWFIKEVFDLHGKLACGAGVTRE
                     DAEALRDMLVNWTLDHISVKDKRIAETLNSFGGPLRK"
     gene            512..1144
                     /locus_tag="P5219_02455"
     CDS             512..1144
                     /locus_tag="P5219_02455"
                     /inference="COORDINATES: protein motif:HMM:NF016398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HRX25649.1"
                     /translation="MDSGTLSDFHLDVLKEVSNIGAGNAATSLSSLLGRAVDMRVSRV
                     VPMPFNGIVDYAGGAERMILSVFVRIEGGISGNMLFVMGTSDALALISHMTGASGGEG
                     FTDMGLSVIHEVGNIMIGSYITALSDFLGFELTPSVPSLAMDMAGAILAFSLSEIGRS
                     SDTAIVIDGCLSLDGNPLSEEESSHIFLLPDPDSFGRIFQALGVAGHGGR"
     gene            1131..1619
                     /locus_tag="P5219_02460"
     CDS             1131..1619
                     /locus_tag="P5219_02460"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HRX25650.1"
                     /translation="MAVVRVGIAESGVVMNPDSISTLGLGSCVGVTLYDEKRRLGGMV
                     HVMLPSTELARGTDFNRSKFADSAVPDLLKQMIRYGADERRIKAKLAGGAQMFSLGGN
                     ADSLLKIGSRNVEACRKVLSALRIPILSEDTGGSWGRTVELFSDSGMLEIRAIGREKK
                     LI"
     gene            1694..3052
                     /locus_tag="P5219_02465"
     CDS             1694..3052
                     /locus_tag="P5219_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRX25651.1"
                     /translation="MSTDRHLLGTEGIGRLLFRLSLPSAVGMIVMSSYNVVDAIFIGR
                     GVGSPGLAAVAICFPLQLLAGAMAVMAGAGGASIISRSLGAGDLERARKAFGTTVSFA
                     SGIGILVSVFVLLFLEQVLRLFGATEAILPHAEAYLSVILFGIPLQMFAMVGNHAARA
                     EGRARIAMTTLLISAILNMILDPVFIFGFGWGMRGAAVATVLSQMVMFLWIFGYFYRG
                     KSALSFVREYLVPSPRLLREIVAVGSSEFVRMAAGSFSIVLINGALVHWGGDLHVAVY
                     GVINRALSFFFMPLMGIAQGFQPILGYNYGAGNLDRARRVIRLAVTASTALALIGFTA
                     AQVFPREIFRLFSGDQDLIAEGVRAMSLISMAFFLIGFQITGSAMFQALGKARPAFLL
                     SLSRQVLLLIPLVLILPGFLGTKGVWISFPLADSGSFLLTLYLFRRELLNLAVSGPVA
                     GD"
     gene            complement(3078..>3705)
                     /locus_tag="P5219_02470"
     CDS             complement(3078..>3705)
                     /locus_tag="P5219_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HRX25652.1"
                     /translation="GSRALSGTPRATASFRSNPLGLAEFTLSRKDPSYVGKAKEVRAF
                     TKALADGKAPGEIAPELEEVFSALEAVPECGAILGKSVSLASAVYARKPGDGSAREQA
                     ASCQRVLGGGANIAEEYATKRYRSNLINWGMIPFVLDGEPPFSAGSWIFVPGVRQAME
                     RGETVFPAYAAGKGSCEKITLETGVLTEDERAVILAGSLINFNRKKTS"
BASE COUNT          621 a         1208 c         1104 g          772 t
ORIGIN      
        1 tttctcccct ctctcccatc tggtacaatg aaccggtgca ctcgacagcg ggcggacttc
       61 cccctgcagg agggtgaaac aatgacagtt cagtggagcg acgacctcgc catcggcatc
      121 ggcatcatcg acgaccagca caaaaaactc gtggagcgca tcgcctcctt ttccaaagcc
      181 gttgaaagcg gcgacaggaa caggatggag gatacggtca actacctcat cggctacgcc
      241 atccagcatt tcggcgccga agagctcatc atgatccgca actgctacga ccagttcaga
      301 gagcaccggg acgagcacag ctggttcatc aaggaggttt tcgacctcca cggcaagctc
      361 gcctgcgggg ccggggtgac ccgtgaggat gccgaggccc tgagggacat gctggtgaac
      421 tggactctcg accacatctc cgtcaaggac aagcgcatcg ccgagaccct caacagcttc
      481 ggcggtcccc tcaggaaata ggatgcatcc catggactcc ggaaccctga gcgattttca
      541 cctcgacgtc ctgaaagagg tgagcaacat cggcgcggga aacgccgcca cgtccctatc
      601 ctccctattg ggaagagcgg tggacatgcg ggtttcccgg gtggtcccca tgccgttcaa
      661 cgggatcgtg gactatgcgg gcggcgcgga gcggatgatt ttgtcggtct tcgtccgcat
      721 cgaggggggc atttcgggaa acatgctctt cgtcatgggc acctccgacg ccctcgccct
      781 catcagccac atgacgggag cgtccggcgg agagggtttc accgacatgg ggctctcggt
      841 gatccacgaa gtcgggaaca tcatgatcgg ttcctacatc acggctctct ccgacttcct
      901 cggcttcgag ctcaccccgt ccgtcccgtc cctggctatg gacatggcgg gggccatcct
      961 ggccttcagc ctctccgaaa taggccggag cagcgacacg gccatcgtca tcgacggctg
     1021 tctttccctg gacggaaatc ccctctctga ggaagaatcg agccatatct tcctccttcc
     1081 cgaccccgat tcgttcggac ggatcttcca ggctctcgga gtggccggcc atggcggtcg
     1141 ttagggtcgg catcgccgaa tcaggagtgg tgatgaaccc cgactccatc tccaccctcg
     1201 ggcttggttc ctgcgtgggg gtgaccctgt acgacgaaaa acggcgcctc ggcggcatgg
     1261 tccacgtgat gctcccttcc acggagctcg ccaggggaac ggatttcaac aggtcgaagt
     1321 ttgccgattc cgccgtgccg gacctcctga agcagatgat ccggtacggg gccgacgagc
     1381 gccgcatcaa ggccaagctc gccggggggg cccagatgtt ctccctgggg ggcaatgccg
     1441 actccctcct caagatcggc tcccggaacg tggaggcatg caggaaggtt ctctcggccc
     1501 tcaggatccc catcctgtcc gaggacaccg gcggctcctg gggccggacg gtggagctgt
     1561 tctccgattc cgggatgctc gagatccggg ccatcggcag ggagaagaag ctcatctagg
     1621 gagacggaaa aagatatatc atcccgggcg cggcgaggat cgccgtgccc tttttgtccg
     1681 gaggcatttt cacgtgagta cagaccgtca tcttctcgga accgagggca tagggaggct
     1741 tctcttcagg ctctcccttc cctcagccgt cggcatgatc gttatgtcgt cctataatgt
     1801 ggtggatgcc attttcatcg gcaggggcgt cggttccccc ggactggcgg cagtggccat
     1861 atgcttcccc ctgcagctgc tcgccggggc catggccgtc atggccggcg cggggggagc
     1921 gtccatcatc tcccgcagcc tcggcgcggg agaccttgag agagcccgga aggccttcgg
     1981 caccacggta tccttcgcct ccggcatcgg cattctcgtt tcagttttcg tcctgctctt
     2041 tctcgaacag gtcctccggc tcttcggcgc caccgaggca attttgcccc acgcggaagc
     2101 ctatctttcc gtgatccttt tcggcatccc cctccagatg ttcgccatgg tgggaaacca
     2161 tgccgcacgg gcggagggca gggcccggat cgccatgacc acgctgctga tctccgccat
     2221 actgaacatg atcctcgatc ctgtattcat cttcggcttc ggctggggga tgcggggagc
     2281 cgccgtggcc acggtgctct cccagatggt gatgttcctg tggattttcg gctatttcta
     2341 cagggggaaa agcgcccttt ccttcgtcag ggagtacctt gtcccctcgc cccggctgct
     2401 tcgggaaatc gtggcggtgg ggtcgtcaga gttcgtcagg atggctgcgg ggagcttctc
     2461 catcgtcctc atcaacggag ccctcgtcca ttggggcggg gatcttcacg tggccgtcta
     2521 cggcgtcatc aacagggccc tttccttttt cttcatgccc ctcatgggca tagcccaggg
     2581 gttccagccc atcctgggct acaactacgg ggcgggaaac cttgacaggg cccgcagggt
     2641 catccgcctc gccgtgacgg cctccacggc cctcgccctc atcggcttca cggcggcaca
     2701 ggtctttccg cgggagatct tccggctgtt ttccggcgac caggacctta tcgccgaagg
     2761 agtccgggcc atgagcctca tttccatggc ttttttcctc atcggattcc agatcacggg
     2821 gtcggccatg ttccaggccc tgggaaaggc ccgtcctgcc tttcttctct ccctctcgcg
     2881 gcaggtgctc ctgctcattc ccctcgtgct tatccttccg gggttcctgg ggaccaaggg
     2941 ggtctggatc tccttccccc tggcggacag cggttccttt ctgctgactc tctacctgtt
     3001 caggagggag ctcctgaacc tggccgtttc cgggcccgtc gcgggggact gacccggaaa
     3061 gggggtcagg tcccttttca ggacgtcttt ttcctgttga agttgatcag tgagccggcg
     3121 agaatgacgg cccgctcgtc ctcagtcagg acgccggtct cgagggtgat cttttcgcag
     3181 ctccccttcc cggccgcgta ggctggaaac accgtctccc cccgctccat ggcctgccgg
     3241 acgcccggca cgaaaatcca gcttccggcg gagaagggag gctcgccgtc aaggacgaag
     3301 gggatcatcc cccagttgat gaggttgctc ctgtacctct tcgtggcata ttcctcggcg
     3361 atgttcgccc ctccgccgag aaccctctgg cagctcgcgg cctgctcccg ggccgaaccg
     3421 tccccgggct tccgggcata cacggcggaa gccaggctga cggatttccc taaaattgcc
     3481 ccgcactccg ggacggcttc cagggcggag aaaacctctt ccagctcagg agcgatttct
     3541 ccgggggctt tgccgtctgc aagggccttt gtaaaggccc tgacctcctt cgcctttccc
     3601 acgtaggaag gatctttccg ggaaagggtg aattccgcca gccccagggg gttggagcgg
     3661 aagctcgccg tcgccctggg cgtcccgctc aacgctctcg acccc
//
LOCUS       DAOTHD010000092         3627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_30296_length_3627_cov_3.101344, whole genome shotgun sequence.
ACCESSION   DAOTHD010000092 DAOTHD010000000
VERSION     DAOTHD010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30296_length_3627_cov_3.101344"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="P5219_02475"
     CDS             <1..220
                     /locus_tag="P5219_02475"
                     /inference="COORDINATES: protein motif:HMM:NF040249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HRX25653.1"
                     /translation="LNDGRVFNLQETFPKGHPKNPFSSDELLWKFRTLAGKALEDENR
                     VDRIIEAVLHMEDLESFNELTGLLSASD"
     gene            245..1510
                     /locus_tag="P5219_02480"
     CDS             245..1510
                     /locus_tag="P5219_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRX25654.1"
                     /translation="MGKTAAEKIISAHSGVDARAGDFVIADVDLAMIHDTTGPIAIQA
                     FEEYGKERVWDPSKMVVVLDHATPSPNERISNLHALLRNFASAQGIPLFDVGDGVCHQ
                     LVIERGLVSPGGLAVGADSHTCTYGALNVFAFGIGSTDMSGVMLTGKLWLRVPGTMKV
                     HIRGPMEEGTCAKDLMLHLIGKIGSDGADYLALSFCGDTVRGMSLSERMTLSNMAIEC
                     GAKAGLMEADDTTAAHLRALGKSEGIPAFDDEDSRFDGKIEMYAPDVPPQVSLPHSVD
                     AAVPVTEAAGTEISQVYIGTCTNGRLDDLRIAARILAGNHVSPNCRLLVCPPSRKVLA
                     DALAEGIILQLVEAGATILPPGCGPCPGTHLGVPGDGENVLSTANRNFRGRMGNNKSG
                     VYLASPATCAASALEGRITDPRPYLSRKQ"
     gene            1522..2028
                     /locus_tag="P5219_02485"
     CDS             1522..2028
                     /locus_tag="P5219_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HRX25655.1"
                     /translation="MENILAGKAHTFGDNINTDYIIAGKYTKTLDFTSLAEHVFEDIA
                     PGFSKRVRPGDIVAAGRNFGCGSSREQAPIAIKYAGISAILAKSYSRIFFRNAINQGI
                     PCFLCDTDGITQDDTVTVHIGQNFVELAEKGKRIPLEPLSPVMLDILREGGLVEYLKK
                     NGKYILPR"
     gene            2138..3154
                     /locus_tag="P5219_02490"
     CDS             2138..3154
                     /locus_tag="P5219_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRX25656.1"
                     /translation="MKIRNGIFSGLIIAVLAAVLLFAGTAQAEIHLNMGSTSSTSGVY
                     AWCVAAANVINKADNGIQVTVVESGAGMDNLRKISAGVFDFAMAIDLPGSLQLYKGIE
                     GFEGKQFKDVRWLFLRNIFADRLYVRKDSGVASFADLKGKRFCPGIPGSASAAYVMQY
                     DKILGHNIQLVPSALGDAITALKENRIVGLQKSSGLNSMDASLIEVNLTTPLTVVGYS
                     EEDVKKIQEKIPYMTFLKREKGSIREFPDVGPIWEECPIAGAVATASLPEEVGYQIVK
                     AYVEGFDEIAAAYGPIKGFDPVGDYFKNAGDDVVPAHAGLIRYAKEKGIDVPERFIPP
                     EYKK"
     gene            3227..>3627
                     /locus_tag="P5219_02495"
     CDS             3227..>3627
                     /locus_tag="P5219_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter permease"
                     /protein_id="HRX25657.1"
                     /translation="MSLTSIGRIKTKVIFTLGFFLAVFQLLAPIYLTSLLDIQFRAIH
                     VAFGLSIGFLYFPFRTRREEEPPDEGISLYDLLLIAVLLAANLNAFIKALDIYMGTTG
                     PSLFDLILGGTLTLLVLEAARRSVGLAIPLMV"
BASE COUNT          853 a         1050 c         1013 g          711 t
ORIGIN      
        1 cctgaacgac ggccgggtct tcaacctcca ggagaccttc cccaaggggc acccgaagaa
       61 tcccttctct tccgacgagc tgctctggaa gttccgcacg cttgccggaa aagcactcga
      121 agacgagaac cgtgttgaca ggatcatcga ggccgtcctt cacatggaag acctggaaag
      181 cttcaacgaa ctgacaggac tcctgtcagc atcagactga agaacaggaa aggaggacga
      241 cggcttggga aagactgcgg ctgaaaagat catatctgcc cattcggggg tggatgcacg
      301 ggcaggtgac ttcgtcatcg ccgatgtgga tttggccatg atccatgaca ccaccggtcc
      361 catcgccata caggcatttg aagaatatgg aaaagagcgg gtctgggacc cttcaaaaat
      421 ggtcgtcgtc ctcgaccatg ccacacccag tcccaacgag cggatcagca accttcacgc
      481 acttctcagg aactttgcat cggcccaggg gataccactt tttgacgtgg gggacggagt
      541 ctgtcaccag ctcgttatcg agaggggcct tgtttctccc ggaggacttg cggtgggggc
      601 cgattcccat acatgcacgt acggcgccct caacgtgttt gccttcggca taggctccac
      661 cgacatgagc ggggtcatgc tgacgggaaa gctctggctc cgcgttcccg gaacaatgaa
      721 ggtccatatt cggggaccga tggaggaggg gacatgcgcc aaggacctta tgctccatct
      781 catcggaaaa ataggctccg acggcgccga ctatctcgcc ctctccttct gcggggatac
      841 tgttcgggga atgtcccttt cggaacggat gaccctgagc aacatggcca ttgaatgcgg
      901 cgcgaaagcc ggactgatgg aggcggatga tacgacggca gcccacctga gggcactggg
      961 gaaaagcgaa ggcatccccg cattcgatga cgaggattcc cggttcgatg ggaagattga
     1021 aatgtacgca cccgacgtcc ccccccaggt cagtctcccc cattcagtgg acgccgccgt
     1081 acccgtaacg gaggctgcgg ggacggaaat cagccaggtc tatatcggca cctgcactaa
     1141 tggaaggctc gacgacctca ggatagcggc acggatcctt gccgggaacc atgtttcccc
     1201 gaactgccgg ctccttgtct gccctccttc gagaaaagta ctggccgacg cgctggctga
     1261 aggcattatc cttcagcttg tggaggccgg agccacaatc cttccacccg gatgcggacc
     1321 ctgcccgggc acgcacctcg gcgtcccggg agacggcgaa aacgtcctct ccaccgccaa
     1381 ccgaaatttc aggggcagaa tggggaacaa caagtccggg gtctacctcg cctctcccgc
     1441 cacgtgcgcc gcaagcgccc tcgaggggcg gatcaccgat ccgcggccgt acctgtcgcg
     1501 gaagcagtga ggaggacaga catggaaaac atactggccg gaaaagccca tacctttggg
     1561 gacaatatca acaccgacta catcatcgcc gggaagtaca cgaaaaccct cgacttcacg
     1621 agccttgcgg aacatgtatt cgaggacata gcccccggct tttccaagag agtccgcccg
     1681 ggagacatcg tggccgcggg acgcaacttc ggatgcggat cgtcccggga acaggccccc
     1741 atcgccatca agtacgccgg gatctcagcc atcctcgcaa aatcctattc ccgcatattt
     1801 ttccgcaacg cgatcaacca gggtattcca tgctttctct gcgacacgga cggcatcacc
     1861 caggatgata cggttaccgt ccatatcggg cagaattttg tcgaactggc agaaaaggga
     1921 aaaaggatac cactcgagcc cctgtctccc gtgatgctcg acatactaag ggaaggcggg
     1981 cttgttgaat acctgaaaaa gaacgggaaa tacatccttc cccggtgacc ccggaggaaa
     2041 agcgaaagga gtgtggttca ggaagagtat gtgcgtaccc ctgcggagat attctctcaa
     2101 aggcgaaaag agaaaaacgg acaggaggga tatgaaaatg aaaatcagga acggtatttt
     2161 ttcaggattg atcattgcag ttctggcggc agtccttcta tttgcgggta ctgctcaggc
     2221 tgaaatccat ctgaacatgg ggagcacatc ctccacttcg ggcgtgtatg cctggtgcgt
     2281 ggcagctgcc aacgtcatca acaaggccga caacggcatc caggttaccg tcgtcgaaag
     2341 cggtgccggt atggacaacc tcaggaaaat aagtgcggga gtgttcgatt tcgcaatggc
     2401 cattgacctt cccggatcgc ttcagctcta caaggggatc gaaggttttg aaggcaagca
     2461 gttcaaggat gtacggtggc tcttcctgag aaacatcttt gccgacaggc tgtacgtccg
     2521 caaagattcg ggtgttgcat cctttgccga ccttaagggc aaacggttct gtcccggcat
     2581 tcccggttcg gcatctgcag catacgtcat gcagtacgac aagatcctcg gacacaatat
     2641 ccagctcgtt ccatcagccc ttggggatgc aataaccgcg ctcaaggaga accggattgt
     2701 cggcctccag aaatcaagcg gcctcaatag catggacgcc tccctcatcg aggtcaatct
     2761 cacgacaccg ctcaccgtcg tgggatattc cgaggaagac gtaaagaaga tccaggaaaa
     2821 gatcccctac atgaccttcc tcaagcgtga aaagggaagc atcagggaat ttccggacgt
     2881 aggccccata tgggaggagt gccccatcgc cggagctgtc gccacagcct ccctcccgga
     2941 agaagtgggc taccagatcg taaaggccta cgtggaaggc tttgacgaaa tcgccgcggc
     3001 ctacgggcca ataaagggct tcgacccggt gggagactac ttcaagaacg cgggagacga
     3061 cgttgtgcca gcccatgcag ggctcatacg gtatgcgaag gaaaagggaa tcgacgttcc
     3121 ggagcggttc attcctccgg agtacaaaaa atagcgccgt ctgcccctta tctcggccgg
     3181 ctgcgcgtcc tatgcgcagc caggccccct gcgggaggga tgaactgtga gcttaacttc
     3241 aatcggccgg ataaagacaa aggtcatttt cacgctcggc ttttttctcg ccgtctttca
     3301 gctcctggcc ccgatctatc tgacgagtct cctcgacatc cagttcaggg caatccacgt
     3361 tgcattcggc ctctccatag gttttctcta ctttccgttc cgaaccagga gagaagaaga
     3421 gcccccggat gagggaattt cgctgtacga cctgcttctg attgcggttc ttctggcagc
     3481 aaacctgaac gcattcatca aggcactgga tatctacatg ggcaccaccg ggccgtcatt
     3541 attcgacctg atcctcggag ggaccctcac gctcctcgtc ctggaggccg cccgaaggtc
     3601 cgtggggctg gccataccgc ttatggt
//
LOCUS       DAOTHD010000093         3626 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_30299_length_3626_cov_4.544385, whole genome shotgun sequence.
ACCESSION   DAOTHD010000093 DAOTHD010000000
VERSION     DAOTHD010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30299_length_3626_cov_4.544385"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="P5219_02500"
     CDS             <1..847
                     /locus_tag="P5219_02500"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRX25658.1"
                     /translation="FVLLGAMLWGTTGTSQALAPAGATPLAVGALRVAIGGAALLTVA
                     LLRGSFSSGGRWPFAPTVISILATASYQPFFFTGVSKTGVAVGTLVTLASSPVITGAL
                     GYLVFGEKPERKWYASSCLAIIGCSLLTLSGEGGGTVDPVGVLFSLGAGFSYSVFVLG
                     SKRLLRDHGADAVNGLVFSAAGLVLLPVLLASPPAWLLQPRGLLVAFHLGLIATALAY
                     YLFLKGLSTVPASTSVTLSMAEPLTAALLGVLLLGERMNLTGAAGVVLLFSGVGLLAL
                     RSRKG"
     gene            1051..1446
                     /locus_tag="P5219_02505"
     CDS             1051..1446
                     /locus_tag="P5219_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRX25659.1"
                     /translation="MKLLKTYLDRCVQCGACMSACSKAWAKEDSPELSRIRVKAVAGM
                     TDIRVCDQCGGCIAVCPTMALSRDKNGVVQIDRKKCTSCLMCVEFCPSASMYFSDRIP
                     SPYKCVACGICVRACPEGALEIIDTGKEG"
     gene            1451..>3626
                     /locus_tag="P5219_02510"
     CDS             1451..>3626
                     /locus_tag="P5219_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HRX25660.1"
                     /translation="MATHDMKLLAEWSYDRAPLHRGYTRKYLVIDLGDGNGEYSHAIH
                     DIDEDMIGTYTGGRGFGLKILWDAVTPESRWNSPENALVISGGPICGITQYPGTGKTY
                     TVFLSPLTDQTYNSNAGGYFGPYLKASGFDALAVTGKAPEDVVVSIDGDEGKIRIYAA
                     PTEERNAYDVTETLHDRFAEDEDDKVSISVVSAGRAAEHSYWGALNVSFYDPRRQVAR
                     LKQAGRGGGGTVLRDKGILALVVRKRGFTGTENAPADLAALQKVGTKLHREIRNNDDV
                     QCIMRTTGTTHLNKIMNDYDILPVNNFKFGRSEAIGAITPEEYKKHFTQGYADGCWYG
                     CSMACAKTVDGFELRTGPDRGKKVTVDGPEYETAASLGPNVGIFDPLWTIEANYYADH
                     YALDTISLGTGLAFVCECYELGLLTKEHTNGLELRFGAGDQLMELIHRMMDGRDEFAA
                     AVGKGIRKMKDIFAERYGAGREIMEQIGMEGQGLEVSQYRCQESVAQWGGYFLTLKGP
                     QHDEAWVIFMDMVNKQIPTFEDKAEALFYFPNFRLWFSLVGVCKLPWNDVEPEDNRIR
                     YKGIEAAKVPEHVQNYLDIYEAVTGTPLSKEDMILQSERVYNYERIFNYRMGKGTRKE
                     HNIPDRALGPVFPDEWNVRKEYFEKKLEEAGISGEGLTVEEKISRLQAHRRGEWEKLV
                     DAVYARRGWDRNGVPTKETVERLGLDSPEVLDLLEPYWRSGRR"
BASE COUNT          747 a         1163 c         1079 g          637 t
ORIGIN      
        1 ttttgtactc ctcggcgcca tgctgtgggg gaccaccggg acgtcccagg ccctggcccc
       61 cgcgggagcc acgccgctgg cagtgggcgc cctccgggtg gccatcggcg gagccgccct
      121 tctcaccgtc gcccttctcc ggggatcctt ctcgtcggga ggtcgctggc cctttgcacc
      181 gacggtgatc tccattctgg ccacggcgtc ttaccagccc ttctttttta ccggtgtctc
      241 gaagaccggg gtcgccgtgg ggaccctggt caccctggcc agttccccgg tgattacggg
      301 agccctggga tacctcgttt tcggggaaaa gcccgaaaga aaatggtatg catccagctg
      361 cctggccatc atcggctgtt cgctcctgac cctttcgggt gagggaggag ggaccgtgga
      421 tcctgtgggg gtcctgtttt ccctgggggc aggcttttcc tattccgtgt tcgtcctcgg
      481 aagcaagcgg ctgctccggg accacggggc ggatgcggtg aacggccttg ttttttccgc
      541 cgccggactc gttctgcttc cggtgctcct cgcttctcct cccgcctggc ttctccagcc
      601 ccgggggctt ctcgtcgcct tccatctcgg gctcatcgcc acagccctcg cctattacct
      661 gttcctgaag gggctgtcca ccgtgccggc ctccacgagc gtcacccttt ccatggcaga
      721 gcccctgacg gccgcccttc tcggcgtcct gctcctgggg gagcggatga acctgaccgg
      781 agccgcgggg gttgtgctgc tcttttccgg tgtgggcctg ctggccctca gaagcagaaa
      841 aggataatcg gtttttttct tcgttggcaa aagggagcaa aatcacccga aaccgtcaaa
      901 aaagtgtcct tcccgtcctt ttccgagtaa aataaaaaga taatgggtgt attgtgtttt
      961 ttcaaaacct gtccatcggc ttatttttca ctgtctgtac aaagtatgga ttcaagaatc
     1021 agttgctatc tcagagaagg agtgttgacc gtgaaactac tgaagacata ccttgaccgg
     1081 tgcgtgcagt gcggagcgtg catgagcgcc tgttcgaagg cgtgggcgaa ggaggactcc
     1141 cctgagctct cccgaatccg ggtgaaggcc gttgcgggca tgacggacat ccgtgtgtgc
     1201 gaccagtgcg gcgggtgcat cgccgtctgc ccgaccatgg ccctctcccg ggacaaaaac
     1261 ggcgtcgtgc agatcgacag gaagaagtgc acctcctgcc tcatgtgcgt cgaattctgt
     1321 cccagcgcct ccatgtactt ctccgaccgc atcccgtccc cctacaagtg cgtcgcctgc
     1381 ggcatctgtg tacgtgcctg ccccgaaggg gcccttgaaa tcatagacac cgggaaggag
     1441 gggtagggcc atggccaccc acgacatgaa gctcctcgcc gaatggagtt acgaccgggc
     1501 ccccctgcac cggggctaca cccgcaaata cctcgtcata gacctcgggg acgggaacgg
     1561 ggagtacagc catgccatcc atgacataga cgaggacatg atcggcacct acaccggagg
     1621 caggggtttc ggcctcaaga ttctctggga cgccgtcacc ccggagagcc ggtggaactc
     1681 ccccgaaaac gccctcgtca tctccggggg ccccatctgc ggcatcaccc agtaccccgg
     1741 caccggcaaa acctacaccg tcttcctgtc gcccctcacg gaccagacct acaacagcaa
     1801 cgccggaggg tacttcggtc cctacctcaa ggcgtcgggc ttcgacgccc tcgccgtcac
     1861 ggggaaagcg ccggaagacg tggtcgtctc catcgacggc gacgaaggga agatccgcat
     1921 ctacgcggcg cccacggagg agcggaacgc ctacgacgtc accgaaactc tccacgaccg
     1981 tttcgccgaa gacgaggacg acaaagtctc catctccgtc gtcagcgccg gccgtgccgc
     2041 cgagcacagc tattggggag ccctgaacgt cagcttctac gacccccgga ggcaggtcgc
     2101 ccggctcaag caggccggac ggggaggggg aggcaccgta ttgcgggaca aggggatcct
     2161 cgccctcgtc gtccggaaaa ggggcttcac gggaaccgaa aacgctcccg ccgacctcgc
     2221 cgccctccag aaagtgggga ccaagctcca ccgtgaaatc cggaacaacg acgacgtgca
     2281 gtgcatcatg cggaccaccg gcaccacgca cctcaacaaa atcatgaacg actacgacat
     2341 cctgccggtg aacaacttca agtttggcag atcggaggcc atcggggcca tcaccccgga
     2401 agaatacaaa aagcacttca cccagggata cgccgacggc tgctggtacg ggtgctccat
     2461 ggcctgcgcc aagaccgtgg acggattcga gctccgcacc ggccccgacc ggggcaaaaa
     2521 agtcaccgtg gacggccctg aatacgaaac cgccgccagc ctcgggccca acgtgggcat
     2581 cttcgacccc ctgtggacca tcgaagccaa ctactacgcc gaccactacg ccctcgacac
     2641 catctcgctc ggcaccggac tcgccttcgt ctgcgaatgc tacgaactgg ggcttctcac
     2701 caaagagcac acaaacggac tggagctgcg gttcggtgcc ggagaccagc tcatggaact
     2761 catccaccgc atgatggacg gccgggacga attcgccgcc gccgtcggca aaggcatccg
     2821 gaaaatgaag gacatcttcg cggaacggta cggcgccggc cgggaaatca tggaacagat
     2881 cggcatggaa ggccagggac ttgaagtctc ccagtaccgg tgccaggaat ccgtggccca
     2941 gtggggaggg tacttcctca ccctcaaggg cccccagcac gacgaagcgt gggtcatctt
     3001 catggacatg gtgaacaaac agatccccac cttcgaagac aaagcggaag ccctgttcta
     3061 cttccccaac ttccgcctct ggttctccct cgtgggagtg tgcaagctcc cgtggaacga
     3121 cgtggagccg gaggacaacc ggatccggta caaaggcatc gaagcggcga aagtcccgga
     3181 gcacgtgcag aactaccttg acatctacga agccgtcacc ggcacaccgc tgagcaaaga
     3241 agacatgatc ctccagtccg agcgggtcta caactacgag cggatcttca actaccggat
     3301 ggggaaggga acgcggaaag agcacaacat ccccgaccga gccctcggtc ccgtcttccc
     3361 ggacgaatgg aacgtgagga aagaatactt cgagaaaaaa ctcgaagagg cgggcatctc
     3421 cggagaagga ctgacggtgg aggagaagat ctcccgcctg caggcccacc ggaggggcga
     3481 atgggagaag cttgtggacg cggtatatgc ccggaggggc tgggacagaa acggcgtgcc
     3541 gacgaaagag acggtggagc gccttggtct ggacagcccc gaggtcctcg accttctcga
     3601 accctactgg aggtccggga gaaggg
//
LOCUS       DAOTHD010000094         3626 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_30302_length_3626_cov_3.346682, whole genome shotgun sequence.
ACCESSION   DAOTHD010000094 DAOTHD010000000
VERSION     DAOTHD010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30302_length_3626_cov_3.346682"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1587)
                     /locus_tag="P5219_02515"
     CDS             complement(<1..1587)
                     /locus_tag="P5219_02515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRX25661.1"
                     /translation="MKRPVELTDLLRYRFLSAPEFSPEGNAICFFVHQADYDNNGYLS
                     NLWLYDLVSDSLRQLTASGREKAFAWMPGGRSILFSSGRNDAKKTETALYLIDISGGE
                     ARHLRTVPRVLSSLSPLPDGSLLALGVDEPAFDNPYDADMMLFDQVPFCSNGKGFTGR
                     KRKRLYSYGPDGTEKQLTPDLLDAENYTLSEDGKTVLVWGPIFKDVRRQYSALLEIEL
                     PDGTVREILPGTDFSCKWAGWLDGRILVTGSDYAAYGINENVKFHLVGDGKTVCITPG
                     LDRGLRNSVGSDCRYGSTDLNLAFSSEAGRAWFVSTDNYRSHLHTVDSTGKIEQFTSE
                     LFSVDDWRINGGRAAVVGMKGLQLQELYLADGREERQLTRFNDWVAEECLLSVPEHVT
                     VDNGTGEPLDGWYMKPVNFEDGKKYPSILNIHGGPKFAYGDVFFHEMQYWTSKGYAVF
                     FCNPRGSDGKGNGFDDIRGKYGTVDYDDLMTFTDWIAENVPFVDKERLAVTGGSYGGY
                     MTNWIIGQTDRFRAAASQRSI"
     gene            complement(1633..1905)
                     /locus_tag="P5219_02520"
     CDS             complement(1633..1905)
                     /locus_tag="P5219_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRX25662.1"
                     /translation="MIKVLFFATIRDYTREKETVAEGSGTVGDLLLELSDRYGGEFRD
                     EAFDGDTISDRVIVLVNGRHIAHTGGAETRLSEGDTVAVFPIIGGG"
     gene            complement(1999..>3626)
                     /locus_tag="P5219_02525"
     CDS             complement(1999..>3626)
                     /locus_tag="P5219_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HRX25663.1"
                     /translation="IFANGPLTGKGAPSAGRYIVVTKSPRTGCIASSNSGGFWGAELA
                     RAGWFMVILEGKAPKPTYLWINDDTVELRDAAAVWGKDSHASTDALLDDVGDSKAKVT
                     CIGPAGERLSKLAAIMNDKNRAAGRSGVGAVMGSKNLKAVVVKGSARPYVENQDEMKA
                     VLAGAMEKLKTNPVTSQGLPTYGTAVLVNIINQLGGYPKNNWQGAYFPEADTQSGETL
                     AKDFLTKRYSCFGCPIGCGRVTRVGDREGEGPEYETIFAFGVCCGVERLEPIIEANYL
                     CNEYGLDTISAGVTIAAAMELYEKGFIRKEELEGGPELRFGNEEAITYWTKKMGLVEG
                     LGRKLAEGSYRLCEMYGHPEFSMTVKKQEMPAYDGRAIQGIGLNYATSNRGGCHVRGY
                     TISPEVLGLPEKLDPVDISTKPTWVMIFQNLTATIDASGMCLFTSFALGADDYAAFLK
                     AATGLDFDGAAVLQAGDRIWNIERMFNLREGLDPTNADTLPDRLLKEPIPDGPSKGMV
                     SRLPEMLPEYYKARGWDVKGLPTDTKLRELALK"
BASE COUNT          624 a         1028 c         1152 g          822 t
ORIGIN      
        1 gatgctcctc tgggatgcgg cggccctgaa acggtccgtc tggccgatga tccagttggt
       61 catgtaccct ccgtaggacc ctccggtgac ggccagcctc tctttgtcga cgaagggaac
      121 gttttccgct atccagtcgg taaatgtcat gaggtcatcg tagtccacgg tgccgtattt
      181 tccccggatg tcgtcgaacc cgttgccctt gccgtcgctt cccctggggt tgcagaagaa
      241 gacggcatag ccttttgacg tccagtactg catttcatgg aagaagacgt cgccgtaggc
      301 gaatttcggc cctccgtgga tattgagaat cgatgggtac tttttcccgt cctcgaagtt
      361 caccggtttc atgtaccatc cgtcgagagg ttctcccgtg ccgttgtcca ccgttacatg
      421 ctccggaacg gagagaaggc attcctcagc cacccagtcg ttgaagcggg tgagctgccg
      481 ctcttccctg ccgtcggcaa ggtaaagctc ctggagctgc agtcccttca tgcccaccac
      541 ggcggccctg cctccgttga tcctccagtc gtccacggag aaaagctccg aggtgaactg
      601 ttcaattttt cctgttgaat caacggtgtg caggtgggac cggtagttgt ccgtggagac
      661 gaaccaggcc ctcccggctt cggaggagaa ggcaaggttc aagtcggtcg agccgtagcg
      721 gcagtcggag ccgaccgagt tcctgagtcc cctgtcgagc ccgggggtga tgcagacggt
      781 tttcccgtcg cccaccaggt ggaatttcac gttttcgttg atgccgtatg cggcgtagtc
      841 gcttccggtg accaggatcc ggccgtccag ccatccggcc cacttgcagg aaaagtccgt
      901 tccggggagg atttccctca ccgtgccgtc agggagttca atttcgagga gggcgctgta
      961 ctgcctcctg acgtccttga agatggggcc ccagacgagg accgtttttc cgtcctccga
     1021 gagcgtgtag ttctcggcat ccagaagatc gggggtgagc tgcttttccg ttccgtcagg
     1081 tccgtagctg tagagccgct ttcttttccg gcccgtgaag cccttgccgt tggagcagaa
     1141 gggaacctgg tcgaagagca tcatgtcggc gtcgtaggga ttgtcgaagg ccggctcgtc
     1201 caccccgagg gcgagcaggg agccgtcggg cagcggcgag agggaagaga ggactctcgg
     1261 aacagtgcgg agatgcctgg cttcgccgcc ggagatgtcg atgaggtaga gggccgtttc
     1321 cgtctttttc gcgtcgttcc ggccgcttga gaagaggatg gaacgtccgc cgggcatcca
     1381 ggcgaaggct ttttccctgc cggaggcggt cagctgccgg agggagtccg aaacaagatc
     1441 gtacagccag aggttggaga gatagccgtt gttgtcgtaa tcggcctggt ggacgaaaaa
     1501 gcagatggca ttgccttcag gggagaattc gggcgctgac agaaaacggt accgcagaag
     1561 gtctgtgagt tcaactggtc ttttcaactg ttctttctcc tttgggataa tgtcgtcggc
     1621 acggggagga aatcagcccc ctcctatgat ggggaagacg gcaacggtgt ctccttcgga
     1681 gagacgggtt tccgctcctc cggtgtgggc gatgtgccgg ccgttcacga gcacgatcac
     1741 cctgtcggag atggtgtcgc cgtcgaaggc ttcgtcgcgg aactccccgc cgtagcggtc
     1801 ggaaagctca agaaggagat ctcccacggt tccggagcct tccgcgacgg tttctttttc
     1861 ccgggtgtag tcccggatgg tggcgaaaaa caatactttt atcatggaaa catgatatcc
     1921 gaaaaagggg cgtttttcca gagcggcaaa aaagcccctt cccggaattc cgggaagggg
     1981 ctccactgaa cgtttctttt acttcagggc aagctctctg agtttcgtgt cggtgggaag
     2041 gcctttgacg tcccagccgc gggccttgta gtattccgga agcatctcgg gaagcctgct
     2101 gaccatcccc ttggaggggc cgtcgggaat gggttccttg agcagcctgt cgggaagggt
     2161 gtccgcattt gtcggatcga ggccttccct gaggttgaac atccgttcga tgttccagat
     2221 gcggtctcct gcctggagca ctgcggcgcc gtcaaagtcg agcccggtgg ccgccttgag
     2281 gaaggccgca taatcgtcgg cgccgagggc gaaggaggtg aaaaggcaca tgccggaggc
     2341 gtcgatggtc gcggtgaggt tctggaagat catgacccat gtgggctttg tgctgatgtc
     2401 cacgggatcg agcttctccg gcaggccgag gacctcgggg gagatggtgt agcctctcac
     2461 gtggcatccg ccgcggttcg aggtggcgta gttcaggccg attccctgga tggcccgtcc
     2521 gtcataggcg ggcatttcct gcttcttgac ggtcatggag aactcggggt gcccgtacat
     2581 ctcgcacagc ctgtaggaac cttctgcgag ctttctgccg aggccctcaa cgagtcccat
     2641 ttttttcgtc cagtatgtta tggcttcctc gtttccgaaa cggagttcgg gaccgccctc
     2701 aagctcttcc ttcctgatga agcccttttc gtagagttcc atggccgccg cgatggtgac
     2761 accggcgctg atggtgtcga ggccgtattc gttgcagagg tagttcgcct cgatgatggg
     2821 ttcgagccgt tccacgccgc agcagactcc gaaggcgaag atggtctcgt actcggggcc
     2881 ttcaccctcc cggtcaccga ccctggtgac ccggccgcag ccgatggggc atccgaagca
     2941 ggagtatctc ttggtgagga agtccttggc gagggtttcg ccggactggg tatccgcctc
     3001 ggggaagtac gctccctgcc agttgttctt gggatacccg ccgagctggt tgatgatgtt
     3061 caccaggact gccgttccgt aggtggggag tccctggctg gtgacgggat tggtcttgag
     3121 cttttccatg gctcccgcaa gcacggcctt catctcgtcc tggttttcca cgtaggggcg
     3181 ggcgctcccc ttgaccacca ccgccttgag gtttttggat cccatgacgg caccgacgcc
     3241 gctgcggcct gctgcacggt tcttgtcgtt cattatcgcc gcgagttttg aaagtctttc
     3301 accggcggga ccgatgcagg tgaccttcgc cttggaatcg cccacatcat cgaggagggc
     3361 atcggtggac gcatggctgt ccttgcccca gacggcggcg gcgtcacgga gctccaccgt
     3421 gtcgtcgttg atccagagat aggtcggttt tggggccttg ccttccagga tgaccatgaa
     3481 ccatcccgcc ctggcgagct cggctcccca gaatccgccg gagttgctgc tggcgatgca
     3541 tcccgtgcgg gggctcttgg tgacgacaat gtaccgtccc gctgaaggag cgcctttgcc
     3601 ggtaaggggg ccgttggcga atatga
//
LOCUS       DAOTHD010000095         3605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_30545_length_3605_cov_2.544789, whole genome shotgun sequence.
ACCESSION   DAOTHD010000095 DAOTHD010000000
VERSION     DAOTHD010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30545_length_3605_cov_2.544789"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="P5219_02530"
     CDS             complement(<1..253)
                     /locus_tag="P5219_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRX25664.1"
                     /translation="MLDALKEGAYFVDRERRITYWNKAAERLTGFRSEEVVGHRCSEN
                     ILVHVDCQGKNLCKAGCPLLATMEDTLTREAEVFFHHRRG"
     gene            complement(418..1698)
                     /locus_tag="P5219_02535"
     CDS             complement(418..1698)
                     /locus_tag="P5219_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRX25665.1"
                     /translation="MTYLMTAAFLVCFFMGIPLALVMGITGIVVLIAMGVPLEVVAQR
                     MFTGIDSFPLMAVPFFILAGDLMNRGGTTVRIIGFANSLVGHIRGGLAHACVVANMIF
                     AGISGSSVADASAIGSIMIPSMEKSGYDLDFSAALNSVAATIGPIIPPSIIMVIYGVS
                     VNVSVGGLFAAGFVPGILMGLALMIVVTRVAKKKNYPTSEGFSGKRVAAEFRSSVWAL
                     MAPIIILGGILGGVFTPTEAAAVAVIYSFFVGKFIFREIAWKDVPHILFQSGITTGAI
                     LLIISLANVFAWVIAANQVPVKLSALFLSATSNPYVFLLIVNILLLIVGMFMETGAAI
                     ILLAPILAPIAAKLGINPLHFGFMMVLNLAIGMATPPVGVCLFVSCGITGLSLEKVSA
                     AAMRFVIALLGVLLLVTYVAPISLFLPKLLGFIR"
     gene            complement(1695..2228)
                     /locus_tag="P5219_02540"
     CDS             complement(1695..2228)
                     /locus_tag="P5219_02540"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRX25666.1"
                     /translation="MKLLNGFLEVFDRAAFILVAALMGAMGVIAFMAVFYRFVLHDPI
                     TWSEEAARYMMVWVTFLGAEYAMGKGRHIGVTMFVEKLPEGARRKVTFAAEIIIMVFL
                     AAVTVQGIKLMIGLWGQTSPAMDFPMWMPYLAIPVGSVYMFMHLLYLVLSRSSQSIST
                     ADLELEAALKGKDGAGQ"
     gene            complement(2327..3358)
                     /locus_tag="P5219_02545"
     CDS             complement(2327..3358)
                     /locus_tag="P5219_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRX25667.1"
                     /translation="MKKMVKVLCALVLVVALAGAAAAENFTLKLANAGPADPEDRTVI
                     AVELFRNHVQNKTGGSVKIEVFHASQLGNEKEILEGLKMGTIELGTITTGPVPTLFKP
                     IMVFDIPYLFPNQVVAWDVLDGPFGRKLMEEMRKVTGIRTLAISENGYRHFVTSEKQI
                     KSVADMKGLKIRTMENPAHMKMVEALGASPTPVAFGELYMALQQGVVDGCELPMTLTN
                     NGKYYEVQKHTILDGHLYNPLIMFVNDKVWGKLSEDQKAALYEGAQLFNIAQRAISER
                     QVQSGILNVTEKGMTVYVPTAAQLNEFRSVSQPPVLDYVKSQAGEQWVKEALEAVAAA
                     EAKMAGMLK"
BASE COUNT          778 a         1060 c         1051 g          716 t
ORIGIN      
        1 ggcccctccg gtgatggaag aacacctccg cctcccgggt gagcgtgtcc tccatagtgg
       61 cgagaagggg acatcctgct ttgcagagat tttttccctg gcaatccacg tggacgagaa
      121 tgttttcgct gcaccggtgt cccaccactt cttcgctccg gaacccggta agccgctccg
      181 cagccttgtt ccagtaggta atccgccgct cccggtccac gaaatatgct ccttctttca
      241 gagcgtcaag catccgctgg atatcccggc gcaatccatc cccacccctt cttgcgtttt
      301 ttattatgat acggaatatc aggaaaaaag caagtcgatg ggatataaca atctcccgct
      361 cgaagagcgg gagattgtgc agtccgggat tgtttccagg agaaccggcg agccgcctta
      421 ccggatgaac cccagcagtt tcggcaggaa gagagagatt ggagcgacgt aggtcacgag
      481 aagaagcact ccgagaaggg cgatcacgaa ccgcatcgcc gctgcggaca ctttctccag
      541 gctcaggccc gtgatgccgc agctcacgaa aagacacacc cccaccggcg gcgtcgccat
      601 cccgatggca aggttgagca ccatcatgaa accgaaatgg agagggttaa ttcccagttt
      661 cgccgcgatg ggcgcaagga tgggagccag gaggatgatg gccgccccgg tctccatgaa
      721 catccccacg atgagaagca ggatgttcac gatgagaagg aagacatagg ggtttgacgt
      781 ggccgagagg aacagggccg acagcttcac gggaacctgg ttcgctgcga tgacccaggc
      841 aaacacgttg gcgagggaga tgatcagcag gatggccccc gtggtgatgc cgctctggaa
      901 caggatgtgg ggaacgtcct tccatgcgat ctcccggaaa atgaacttgc cgacgaagaa
      961 ggagtagatc accgcaacgg cggctgcctc cgtgggggtg aacacgccgc cgaggattcc
     1021 tcccaggatg atgatgggag ccatcaatgc ccataccgat gacctgaact cggcggcaac
     1081 cctctttcct gaaaagccct cggaggtggg gtagttcttt tttttcgcca cccgggtgac
     1141 cacgatcatg agggcaaggc ccatgaggat tcccggaaca aaccccgcgg cgaagagtcc
     1201 tcccaccgac acgttcaccg acacgccgta gatgaccatg atgatgctcg gcgggatgat
     1261 ggggccgatg gtggccgcca cgctgttgag ggcagcgctg aagtcgaggt cgtagccgct
     1321 cttctccatg gaggggatca tgatggagcc gatggcggaa gcgtcggcca ccgatgaacc
     1381 gctgattccg gcgaagatca tgtttgccac gacacacgca tgggccagcc cgccccggat
     1441 atggccaacc aggctgttgg cgaaaccgat gatcctcacc gtggttcctc cccggttcat
     1501 caggtctccc gcaagaatga agaacggaac cgccatcagg gggaaggagt cgatcccggt
     1561 aaacatcctc tgggcgacga cttcgagggg aacgcccatg gcgataagga cgacgattcc
     1621 cgtgattccc atcacgagag cgagaggaat gcccatgaaa aagcagacga gaaatgcggc
     1681 agtcataagg tatgtcattg cccggcaccg tcctttccct tcagggccgc ttcaagctcc
     1741 aggtccgccg tggatatgga ctgggaagac cggctcagca caaggtacag aaggtgcatg
     1801 aacatataca cggagccgac aggaatcgcc aggtagggca tccacatggg aaaatccatg
     1861 gccggcgacg tctggcccca gaggccgatc atgagcttga tcccctgcac ggtcactgcg
     1921 gcgagaaaga ccatgatgat gatctcggcg gcaaaggtca ctttccgccg ggcaccttcg
     1981 gggagcttct ccacgaacat ggtgacgccg atatgcctgc cttttcccat ggcgtactcc
     2041 gccccgagaa aggtcaccca gaccatcatg taccgtgccg cttcttcgga ccatgtaatg
     2101 gggtcatgga ggacgaacct gtagaagacg gccataaagg ctatgactcc catggctccc
     2161 atgagggctg ccacaaggat gaaggctgcc cggtcaaaca cttcgagaaa tccattcagc
     2221 agtttcactt ctccacctct ccttcagtac gaaaggggga cagggcggtc gtccgcccct
     2281 cccccgcaca tctgcagata ttggaaaacg ctctttattc cggcggctac ttcagcatgc
     2341 cggccatctt ggcttcggca gcggcaaccg cttccagggc ttccttcacc cactgctccc
     2401 cggcctggga cttcacgtag tcgagcacag ggggctggga gacggatcgg aactcgttga
     2461 gctgggccgc cgtgggaaca tagacggtca tgcccttttc cgtcacgttg aggatgcccg
     2521 actggacctg ccgctcggaa atggccctct gggcgatgtt gaagagctgg gctccctcgt
     2581 acagggccgc cttctggtcc tcggagagct tcccccacac cttgtcgttc acgaacatga
     2641 tcagggggtt gtacaggtgg ccgtcaagga ttgtgtgctt ctgcacctcg tagtacttgc
     2701 cgttgttggt cagggtcatg ggaagctcgc agccgtccac cacgccctgc tggagagcca
     2761 tgtacagctc gccgaaggcc acgggggtgg gggaagcgcc gagggcctcc accatcttca
     2821 tgtgggcggg gttctccatg gtcctgattt tcaggccctt catgtccgcc acggacttga
     2881 tctgcttctc gctggtgacg aagtggcggt agccgttctc gctgatggcg agggtacgga
     2941 tgccggtaac cttccgcatc tcctccatga gctttctgcc gaagggcccg tcaaggacgt
     3001 cccacgccac gacctggttc gggaagaggt aggggatgtc gaacaccatg atgggcttga
     3061 acagggtggg aacaggaccc gtggtgatgg tgccgagttc gatggtcccc atcttcagtc
     3121 cttccaggat ctccttttca ttgccgagct gggaggcgtg gaacacctcg atcttcaccg
     3181 aaccgccggt tttgttttgc acgtggttcc tgaacagctc cacggcgatg actgtccggt
     3241 cctccgggtc cgccggtccg gcgttggcca gcttcagggt aaaattctcg gcggcggcgg
     3301 ctcccgccag ggccactacc aggacaaggg cgcacaacac tttcaccatt tttttcatct
     3361 tctctctccg acctttcttt taaatcttgg taaatttgcc ctagcattat aaactaaagt
     3421 ttgaaatccg caagtggcaa tacaacaaat ttcgcgctag acttgtaaaa atttacgtaa
     3481 gggggaaaac aaatgaactg ccttgcgctg ctcggaagcc cgagaaaaga cggcaacacc
     3541 gccgccctcc tcacctcatt tctggacggg gcggcctcga aaggacatac tgtgcgcact
     3601 gtccg
//
LOCUS       DAOTHD010000096         3604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_30558_length_3604_cov_2.535926, whole genome shotgun sequence.
ACCESSION   DAOTHD010000096 DAOTHD010000000
VERSION     DAOTHD010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30558_length_3604_cov_2.535926"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="P5219_02550"
     CDS             complement(<1..191)
                     /locus_tag="P5219_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HRX25668.1"
                     /translation="MDSLVRFGVAVPASLLSEFDLYIRGKGLQNRSEALRQLIRERLS
                     RERWSRGDGIVFGTVTIMY"
     gene            265..885
                     /locus_tag="P5219_02555"
     CDS             265..885
                     /locus_tag="P5219_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25669.1"
                     /translation="MEKFFCVRLLPGKGQKGPAIGGDRKGEACLVLSGRGAVCSPSCC
                     PDAGAFIGIGGDSTVILRDDGGNVLHRYRGMHGEGAGSEFALFPLGRFLCALATGSDL
                     MVPEHARCLSLAGTDLLLAFLLPGEGEKAPFEAVARTRAAENGMFVILAGCSAPPAAF
                     GPDGCDVLPVPGVDGGAEFRLSGDVLSQEGYDQVRRKDLYYSLTAL"
     gene            1022..1774
                     /locus_tag="P5219_02560"
     CDS             1022..1774
                     /locus_tag="P5219_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRX25670.1"
                     /translation="MSGHSKWANIKHRKAAQDAKRGNLFQKLVKAVIIAAKEGGGDPA
                     MNMRLKAAIDRAKAASVPNDNIIRGIKRGTGEIEGAQYEEITYEAYGPNGVAILIEVL
                     TDNRNRAASEIRAVLTRNGGSLGEAGSVAWMFERKGTIEVSGKDLDEEALMTVALEAG
                     AEDLEQQDEGYAVYTDPASLPDVRDALEKAGYSIERAETEMVPKNTVQISDPEKARKV
                     LRLMDLLEENDDTQNVYGNFDIPDEIMESLEG"
     gene            1785..2312
                     /gene="ruvC"
                     /locus_tag="P5219_02565"
     CDS             1785..2312
                     /gene="ruvC"
                     /locus_tag="P5219_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRX25671.1"
                     /translation="MLKCIGIDPGLGRMGFASVVREGQIFRSLSYGCIETPPSETIPV
                     RLNMIYEKLGEQIACCSPDFMSVEKLFFGRNITTAEYVWQARGVALLLAARHKLPVFE
                     PKPSQIKLAVCGYGTAPKEQVQKMVQRILNLDCIPKPDDAADAIAAAVTGFFLFAEEK
                     RTAPSGGRHAAISFG"
     gene            2290..2892
                     /gene="ruvA"
                     /locus_tag="P5219_02570"
     CDS             2290..2892
                     /gene="ruvA"
                     /locus_tag="P5219_02570"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRX25672.1"
                     /translation="MLRFLSGKVVSVSDGLVVLDVAGFGFELFCTGRAAAMCREGEPA
                     MLVTHLQFAETGPSLFGFADERERGLFLKLTTVKGVGGKMAMNILKAASADRVINWIV
                     SSDIESLVKIPGIGRKTAERLCFEMHRHLAGEHADLTLEGVLSGGRSMDLAMEGLRSL
                     GFSQGDVAAVFRKLKNEGAAEDESTAEQLIRMALKELKRN"
     gene            2904..>3604
                     /gene="ruvB"
                     /locus_tag="P5219_02575"
     CDS             2904..>3604
                     /gene="ruvB"
                     /locus_tag="P5219_02575"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRX25673.1"
                     /translation="MTEEIKRFLDPSGGAGEDDTSLRPLSLGEFVGQDKLKEKLSIFI
                     QAARQRGESLDHCLFYGPPGLGKTTLASIIAREMGGQLRVTTGPALEKTGDLAAILSN
                     LEPNDVLFIDEIHRLPSNVEEILYPGMEDFSLHIIVGKGPLANNICLTLPKFTLVGAT
                     TRLGLLTSPLRARFGIVEQLSLYDETELAGIVARGAKVLGITLQKEAAVEIARRSRGT
                     PRVALRLLCRVRDVS"
BASE COUNT          772 a          966 c         1095 g          771 t
ORIGIN      
        1 tcgtacatga tggttacggt gccgaaaacg atgccgtctc ccctggacca cctttccctg
       61 gatagccgtt cccgaatgag ctgccgcagg gcctcggatc tgttctgcag gcccttgccc
      121 ctgatgtaga ggtcgaattc cgaaagaagc gatgccggaa cggcgacgcc gaatcgaacg
      181 agcgaatcca tacaaacccc tcctgttcgt catgtctccg gggagtataa taaactgaaa
      241 ttccctgttt gggggaggat tcttttggaa aaattcttct gtgtcaggct tctgcccggg
      301 aaagggcaaa aaggacccgc cattgggggc gaccggaagg gagaagcgtg tcttgtcctg
      361 tccgggcgag gtgccgtgtg ctctccgtca tgctgtcctg acgcaggtgc ctttatcggt
      421 attggcggcg acagtaccgt cattctccgg gatgacggcg gcaatgtcct tcaccggtac
      481 aggggcatgc acggcgaggg ggccggatcc gaatttgccc ttttccctct gggccggttt
      541 ctctgcgccc ttgccaccgg ttctgatctt atggtgccgg aacatgcacg gtgtctctct
      601 cttgcaggaa cggaccttct cctggcattc cttttgcccg gggagggcga aaaggccccc
      661 ttcgaagccg tcgcaagaac ccgggcggcg gaaaacggca tgttcgtgat tctagcgggt
      721 tgttccgctc cgcccgcagc attcggccct gacgggtgtg acgtgcttcc ggtcccgggg
      781 gtggacggag gtgcggaatt ccgcctttcc ggggatgtac tgtcccagga aggatatgat
      841 caggttcgaa gaaaggatct ctattattcc cttactgccc tgtgagagaa tgttctctgt
      901 gctacaatat cggggcggtg aacatccaca cgccggatct tttttggcgg tgcacaatgt
      961 cttgtttccg gtttcatgaa gcagtgcagt gattcttttg atctatagag gggtgagttt
     1021 cttgtccgga cattcaaaat gggcaaacat caagcacagg aaagcggcac aggatgccaa
     1081 gcggggcaat ctgttccaga aactcgtgaa ggcggttatc atcgcagcca aggagggcgg
     1141 cggggatcct gccatgaaca tgcgcctcaa ggcagccatt gatcgggcca aggccgcgag
     1201 cgtgcccaat gacaacatca tcagggggat caagagaggt accggagaaa tcgagggagc
     1261 ccagtacgaa gaaatcacct acgaagccta cgggccgaac ggagtggcca tccttatcga
     1321 agtcctgacg gacaacagga acagggcggc ttcggaaata cgggccgtgc ttacgagaaa
     1381 cggcggttct ctcggcgagg ccggaagcgt cgcctggatg ttcgagcgta agggaaccat
     1441 cgaagtcagc ggaaaggatc ttgacgagga agccctcatg accgtcgcac ttgaagcagg
     1501 agctgaggat cttgagcagc aggatgaagg atatgctgtg tacaccgatc ccgcatccct
     1561 gccggacgta agggacgccc tggaaaaggc cggctattcc atcgaaaggg cggaaacgga
     1621 gatggtcccc aagaacacgg tccagatcag cgatccggag aaggcacgaa aggtgctccg
     1681 cctcatggat ctcctcgagg agaacgacga cacccagaat gtctacggga acttcgatat
     1741 tcctgatgaa attatggaaa gcctggaagg atagagaaag aacattgctc aaatgtatcg
     1801 gcattgatcc cgggctggga agaatggggt ttgcttcggt ggtccgcgag gggcagattt
     1861 ttcgctccct ttcatacggc tgcatcgaaa cccctccttc cgagactatt cctgtccggc
     1921 tgaacatgat ctatgaaaag ctcggggagc agattgcgtg ctgctccccc gattttatgt
     1981 cggtggaaaa gctctttttc gggagaaaca tcaccactgc ggagtatgtc tggcaggcga
     2041 ggggagtggc cctccttctt gccgcccggc acaaactgcc tgtttttgag cccaaacctt
     2101 cccagatcaa gctggctgtc tgcggttacg gaacggcgcc gaaggagcag gtgcagaaaa
     2161 tggtccagcg catcctgaac cttgactgca tcccgaaacc tgacgatgcc gccgatgcga
     2221 ttgccgccgc cgttaccggc tttttccttt ttgcggagga gaagagaacc gctccttcag
     2281 gaggacgtca tgctgcgatt tctttcgggt aaggtcgttt ccgtttctga tggcctggtt
     2341 gtcctcgacg ttgccgggtt cggcttcgag cttttctgca ccggaagagc ggccgccatg
     2401 tgcagggaag gggagcccgc catgctggtg acccatcttc agtttgccga aacaggtccg
     2461 tccctgttcg gattcgccga cgagagggag cgggggctct tcctgaaact caccacggta
     2521 aagggcgtgg gggggaaaat ggccatgaac atactgaaag ccgcatctgc cgacagggtg
     2581 atcaactgga tcgtctcctc cgatatcgag tctctcgtca aaattcccgg aatcggccgg
     2641 aagacggcgg aacggctctg tttcgagatg caccgccatc ttgcagggga gcacgccgac
     2701 ctcaccctcg aaggagtgct ctctggaggg aggagcatgg atcttgccat ggaagggctt
     2761 cgctccctcg gtttttccca gggtgatgtg gcagccgttt tcagaaaact gaagaacgag
     2821 ggagcagcag aagatgaatc cacggcggag cagctcatcc gcatggccct gaaggaactg
     2881 aagcggaatt gaggtgattc tcggtgacag aggaaatcaa acgtttcctg gatccttccg
     2941 gcggagccgg tgaggacgat acctcgctca ggcccctgtc gctcggtgaa ttcgtggggc
     3001 aggataaact caaggaaaaa ctctccatct tcatccaggc tgcccggcag cggggagaat
     3061 ccctggacca ctgcctgttt tacggtcctc cgggcttggg gaagacgact ctcgcgagca
     3121 tcatcgcgag ggagatgggg gggcagcttc gggtcactac cggaccggct ctcgagaaga
     3181 cgggtgacct tgcggccata ttgtcgaacc tggagcccaa cgatgtgctc tttatcgatg
     3241 aaatccaccg ccttccctcg aacgtggaag agatcctcta cccgggaatg gaggattttt
     3301 ccctgcacat catcgttgga aagggccccc tggcgaacaa tatctgtctg acccttccaa
     3361 aattcactct cgtcggtgcg acaacccgcc tgggtcttct gacgtcacct cttcgcgcac
     3421 ggttcggaat cgtggagcag ctttctcttt atgacgaaac ggaacttgcg gggatcgtgg
     3481 ccagaggggc gaaggttttg ggtattactc tccagaaaga agcggccgtc gaaatagcca
     3541 ggaggtcccg ggggactccc agggtcgctc tccggcttct ctgcagggtt cgtgatgtgt
     3601 ctga
//
LOCUS       DAOTHD010000097         3599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_30618_length_3599_cov_3.313488, whole genome shotgun sequence.
ACCESSION   DAOTHD010000097 DAOTHD010000000
VERSION     DAOTHD010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30618_length_3599_cov_3.313488"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1510
                     /locus_tag="P5219_02580"
     CDS             <1..1510
                     /locus_tag="P5219_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRX25674.1"
                     /translation="HEQIHRVLFRRLEDEAPPCRSSSNLRLHLAGLLLICPDILLLDE
                     PTNHLDSESMEWLEGWLSGFRGTIVAVSHDRRFLDKICTTIAELSLGRISLYKGNFSD
                     YLDERERRIEELRRQERLQKDEIARMEEFIERFRYKASKAASVQSRIKRLEKISLVEV
                     EEDTKRVSFRFPPCVRSGLEVIVLENGEKKYGNHTVFSEASLTVRRGEKIALVGVNGA
                     GKSTLSRILSGTEGLTSGMVRRGHNVRIAFFSQQSSENLDYSKTVWESLSGRNPAWTA
                     QDKRNLLGAFLFSGDDIFKPVPVLSGGEKSRLALLKLLMEESNCLVLDEPTNHLDMQT
                     KDLFQRALLEYDGTLVIVSHDRYFLDNLVTRVVEIASGKLLDYPGNYSWFIEKRKALS
                     SEAEEKRTRPKEKEDPEKDRKRLEAEKRNLLYRKKRKVMDKLEPVEEQISLLEKQQSE
                     RDGLLSDPAFLADSEKVRNLLIQRNDAAKELDSLYGQWERLMAEIEMIDKTG"
     gene            1539..2306
                     /locus_tag="P5219_02585"
     CDS             1539..2306
                     /locus_tag="P5219_02585"
                     /inference="COORDINATES: protein motif:HMM:NF022655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LmeA family phospholipid-binding protein"
                     /protein_id="HRX25675.1"
                     /translation="MRPRNQLRTILIAVTAFFLFFRGIPATAEEEPRGKLLLRHFASE
                     FTPERMTMIIDEEPDATGYVRDIYLDITGCVIGGVRMDSLRIRAMGVQMNPPEEWQAK
                     GLDAREILNVHAFARILEKDLNDNLLSKEFGEDDHWNNLQVDMRPDGIYARGNYLVTV
                     LFRLNILIEIFSKFKIVEMQQVWLHDYTLKVNRVDVPQFITDQAVEQIQPLLDLRKFV
                     FPLKLHSIEYDETCLSIASRVLPEAFQGILYEYEAGN"
     gene            complement(2351..3247)
                     /locus_tag="P5219_02590"
     CDS             complement(2351..3247)
                     /locus_tag="P5219_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRX25676.1"
                     /translation="MKFENIDLQVNKRGFRRFISSWLVRDEDGDRTYLVDTGPGSTWP
                     RVRDAVERHGGGRVDAVLLTHVHLDHAGAAPLAFREYGAHVSAAPKGIPHLLDPSALW
                     KGSVETLGETACLYGEPEPLPREALLADEDLPAGFSAIETPGHASHHRCFVLDEVNRG
                     KTLFAGEAAGIFLEGEEPFPYLRPATPPRFFPEVTLESIERLIPLGCRRICYSHFGAA
                     EGATEMLSFARDQILFWRDIVVDMFRKGFSPDDEEAIFRELLERDPFLAAWRKMEPDI
                     RDREREFIGNSIRGFAGAYGPK"
     gene            complement(3259..>3599)
                     /locus_tag="P5219_02595"
     CDS             complement(3259..>3599)
                     /locus_tag="P5219_02595"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRX25677.1"
                     /translation="RIYKVAVGANGGQKQRVGDSRTPTGTFSVQQIQKASSWTYDFGD
                     GNGPTRGAYGPWFIRLKTPGWSGIGIHGTHDPGSIGTMITQGCVRMRNHELEELKKMV
                     FVGMKVVISE"
BASE COUNT          890 a         1005 c          941 g          763 t
ORIGIN      
        1 acatgaacag atccaccggg ttcttttccg gaggctggaa gatgaggctc caccttgccg
       61 gtcttcttct aatctgaggc tccaccttgc cggtcttctt ctaatctgtc cggacatcct
      121 cttgctggac gaaccgacga accatctaga ctcggagagc atggaatggc tggaaggctg
      181 gctttcaggt tttcgcggga ctattgtggc cgtctcccac gaccggagat ttctggacaa
      241 gatctgtaca accattgcgg aactctctct tggaaggata tccctgtaca agggcaattt
      301 ctccgactac ctcgacgaac gggaaaggcg gattgaggaa ctgcggaggc aggaacggct
      361 ccagaaggat gaaatagccc gcatggagga atttatcgag cggttccgct acaaggcatc
      421 aaaggcggcg tccgtccaga gcaggatcaa gcggcttgaa aaaataagcc tggttgaagt
      481 tgaggaggac acgaagagag tgtccttccg cttccctccc tgcgtccgca gcggacttga
      541 agtaatcgtc ctggaaaatg gtgaaaaaaa atacggaaat cacaccgttt tttccgaagc
      601 atcccttacg gtccgacggg gagagaaaat tgccctggtc ggagtcaacg gagccggaaa
      661 atctactctc tcccgcatcc tcagcggaac ggaggggctt acctccggaa tggtgcggag
      721 aggtcacaac gtccggatag ccttcttttc ccagcaatcc tcggaaaacc ttgattattc
      781 aaaaactgtc tgggaatccc tgtcagggcg caatcccgca tggacggccc aggacaagcg
      841 caacctcctc ggggcttttc tcttcagcgg ggacgatatt ttcaaacctg tcccggtcct
      901 ttcgggagga gagaaatccc gtctggccct tctgaagctc ctgatggaag agtccaattg
      961 cctggttctc gacgagccga caaaccacct cgatatgcag accaaggacc ttttccagag
     1021 agccctcctg gaatatgacg gcaccctggt catcgtttcc catgaccgtt atttcctcga
     1081 caacctggtg accagggtgg tggaaatagc ctcagggaaa ctcctcgact atccgggaaa
     1141 ctattcctgg tttattgaaa aaaggaaagc cctttcttct gaagccgaag agaagcggac
     1201 aagaccaaaa gaaaaggaag accctgaaaa agaccgcaag cgcctcgagg cggagaaacg
     1261 gaacctcctg taccggaaaa aaaggaaagt catggacaaa cttgaaccgg tggaagaaca
     1321 aatttccctc cttgaaaaac agcagtcgga aagggacgga cttctgtccg atccggcctt
     1381 tctcgccgat tcggaaaaag tcaggaacct gctcatccag agaaatgatg ccgcaaaaga
     1441 actcgattcc ctctacggac aatgggaacg tctcatggcg gaaatcgaaa tgatagataa
     1501 aacaggataa tcatgaccac cagggaggac tgtgcagaat gagaccgaga aatcagcttc
     1561 gaaccatact gattgcagtg acggcttttt ttcttttttt tcgtggaatt cccgcgacgg
     1621 cagaagaaga gccccgggga aaactccttc tccggcattt cgcttctgaa ttcacccctg
     1681 agaggatgac catgatcatc gatgaagagc ctgacgcaac aggctatgtc agggacatct
     1741 accttgacat aacggggtgc gtcatcggcg gagtccgcat ggattccctg cggatcaggg
     1801 ccatgggagt gcagatgaac cctcctgagg aatggcaggc aaagggactc gacgccagag
     1861 aaattctcaa cgtccatgcc ttcgcccgta tccttgagaa ggacctcaac gacaaccttc
     1921 tttcaaaaga gttcggcgag gacgaccact ggaacaacct ccaggtggac atgcgccctg
     1981 acggaatcta cgccagggga aactatctcg tgaccgttct cttcagactg aacattctca
     2041 tcgaaatctt cagcaagttc aagatcgtgg aaatgcagca ggtatggctt catgactata
     2101 ctctcaaggt aaaccgggtt gacgttcccc agttcataac ggaccaggcg gtggaacaga
     2161 tccagcccct tctcgacctg agaaaattcg tctttcccct gaagcttcat tctatcgaat
     2221 acgacgagac ctgcctgtct atcgcgagcc gggtacttcc tgaagccttt cagggcatac
     2281 tgtacgaata cgaagcagga aactgaagga aaaaaagaga gtgtgagcga aatcgctcac
     2341 accctctttt tcatttcgga ccgtaggctc cggcgaaccc ccgtatgctg ttgcctatga
     2401 attcccgctc cctgtcccgg atgtcgggtt ccattttcct ccaggcagca aggaagggat
     2461 cccgttccag aagttcccgg aaaattgcct cctcatcatc aggcgagaac cccttcctga
     2521 acatgtccac cacgatatcc ctccagaaaa ggatctggtc ccgggcaaag gaaagcattt
     2581 ccgttgcccc ttcggccgct ccgaagtggg agtagcagat cctcctgcag ccgaggggga
     2641 tcagcctttc aatggactcg agagtcactt cgggaaagaa tcggggcggc gtggccggac
     2701 gaaggtaggg gaaaggctcc tccccttcaa gaaaaattcc cgcggcttcc cccgcaaaaa
     2761 gagttttccc ccggttcact tcgtcgagaa caaagcaccg atggtgggag gcatgacccg
     2821 gcgtctcgat ggccgagaat cctgccggga ggtcttcatc ggccagaagg gcttcccggg
     2881 gaagaggttc aggctcccca tacagacagg ccgtctcgcc gagggtttcc acactcccct
     2941 tccacagggc cgaagggtca agaaggtgag gaatgccttt cggtgcggca gaaacatgag
     3001 cgccatattc ccgaaaagcg aggggggccg ctccggcatg atcaagatga acatgggtca
     3061 gaagaaccgc atcgaccctg cctcccccgt gccgttccac cgcatcgcgg acacggggcc
     3121 aggtacttcc cggtcccgtg tcgacaaggt atgtcctatc cccatcttca tccctgacca
     3181 gccatgagga aatgaagcgg cgaaaccctc gtttgttcac ctgaagatct atgttttcaa
     3241 atttcatagc acctgctcct attccgaaat gacgactttc atgccgacaa aaaccatctt
     3301 tttcagctct tccagttcgt ggttcctcat ccggacgcat ccctgggtta tcatggtgcc
     3361 gatggaccca ggatcatggg taccgtggat tcctattccc gaccatcccg gcgtcttcag
     3421 acgtatgaac cacggtccgt aggctccccg ggtaggtccg ttcccgtcgc cgaagtcgta
     3481 ggtccacgag gaagcctttt gtatctgctg tacggaaaag gtaccggtgg gagttctgct
     3541 gtcgccgaca cgctgcttct gtcccccgtt ggccccgacg gccaccttgt agattcgct
//
LOCUS       DAOTHD010000098         3595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_30664_length_3595_cov_3.857062, whole genome shotgun sequence.
ACCESSION   DAOTHD010000098 DAOTHD010000000
VERSION     DAOTHD010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30664_length_3595_cov_3.857062"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..585
                     /gene="clpP"
                     /locus_tag="P5219_02600"
     CDS             7..585
                     /gene="clpP"
                     /locus_tag="P5219_02600"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HRX25678.1"
                     /translation="MLIPYVVEQTGRGERTYDIYSRLLKDRIIFLGEGIDEHTGNIVV
                     AQLLFLESEDPEKDIHLYINSPGGYVTAGLAIYDTMQYIKSPVSTICIGQASSMAAIL
                     LAGGAPGKRLALPNARVMIHQPLGGAQGQATEVEIHAREILKLRDRLNDILVQHTGQT
                     KKKIAADTERDFFMSPDEALKYGIIDKVIHSR"
     gene            690..1976
                     /gene="clpX"
                     /locus_tag="P5219_02605"
     CDS             690..1976
                     /gene="clpX"
                     /locus_tag="P5219_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRX25679.1"
                     /translation="MFKFDDKGNGRGKGGSSRCSFCGKGQDEVQKLIAGPGVYICDEC
                     VHLCNLILNEEAEETGSEADHFIPMAEAPKPREIKAFLDQYVIGQEWAKKSVSVAVYN
                     HFKRISNNLGKDADVELQKSNVLLLGPTGCGKTLIAQTLAKKLNVPFAIADATTLTEA
                     GYVGEDVENILVKLLQAADYDVKAAQRGIIYIDEIDKLSRKSESASITRDVSGEGVQQ
                     ALLKILEGTVSNVPPKGGRKHPYQDFIQIDTSNILFICGGAFDGVEEIIGRRVNKKLI
                     GFGGDIMGKHDARRYEIMKQIQPEDLMTYGFIPELVGRLPVLVPLEELDADALVRILS
                     EPKNALIRQYQKTFSMEGVSLEFTDEAVRSIAMKANEKNTGARGLRSILENLMMELMY
                     DIPSRSREVRKVIITKEAVEGMKDPILEGKSSKEVA"
     gene            1980..>3595
                     /gene="lon"
                     /locus_tag="P5219_02610"
     CDS             1980..>3595
                     /gene="lon"
                     /locus_tag="P5219_02610"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRX25680.1"
                     /translation="MESTRRCYVLPIRDIVVFPGIIAPLFVGRPRSLKAIEMAMLQDR
                     NILVVAQKDMQIDDPRVEDLYTVGTLCSILQMVRIPDGTTKVLIEGVERVRVESFSRG
                     KEALEADVVPLPWDESLSPNLEALKRSVLEQFDKYVTLHPRIPAEVLVTVMNVDDPRQ
                     ISDLVSSHLSIKVERKQRLLEMIDPEKSLKFILKLLLEEIDILQLEHDIQDKVRQEVE
                     RGHKEYYLREQLKIIQDELGQNEAPSEIEELRGKIEEADMPEVSRNKALHELDRLSKM
                     PVMSAEATVVRTYIDWLVALPWSRFSPDNIDIVRAAKVLEEDHYGLEKVKERILEFLA
                     VRKLASNRMKGQVLCFVGPPGVGKTSLGKSIARALERKFVSMSLGGMRDEAEIRGHRR
                     TYVGAMPGRIIQKIRQAGVRNPVLLMDEIDKMGQDFRGDPSAALLEVLDPEQNGAFTD
                     HFMEVPVDLSDVIFITTANVTHTIPRPLLDRMEVIKIPGYVWQEKTRIAKRHLFPRIL
                     TEHGLSPKQVKVTPKGLEQLISEYTREAGV"
BASE COUNT          891 a          967 c         1053 g          684 t
ORIGIN      
        1 cccagcatgc tgataccgta cgtggtcgag cagaccggca gaggagaacg gacctatgat
       61 atttacagcc ggctcctcaa ggacaggatt atctttctcg gtgaaggaat agatgaacac
      121 acgggaaaca tcgtggttgc ccagctcctc tttctggaaa gcgaagaccc ggaaaaggat
      181 atccacctgt atatcaacag ccccggaggg tatgtcaccg cagggctggc catctacgat
      241 acaatgcagt acataaaaag cccggtatcg actatctgca tcggccaggc atcgagcatg
      301 gccgccattc tcctcgccgg gggcgccccg gggaagcggc tcgctctccc gaacgcgagg
      361 gtcatgattc accagcctct tgggggcgcc cagggtcagg caacggaagt ggagatccac
      421 gcacgggaaa tactgaagct ccgcgaccgc ctgaatgata tacttgtcca gcataccgga
      481 cagacgaaga aaaagattgc ggccgacacg gagagggact tcttcatgtc tcccgacgag
      541 gccctgaaat acgggatcat agataaagtc atccattcga ggtagccatt gctcctcgca
      601 acgaagcctt tccgataaga cttcaaggga gcccgcgagg tgctcccttg ttgctgtatc
      661 ttcggaaggc tccagggagg gagagtttcg tgtttaaatt tgacgataaa ggaaacggaa
      721 gaggaaaggg cggcagttcc aggtgctctt tctgcgggaa ggggcaggac gaagtccaga
      781 agcttatagc cgggccaggt gtctatatct gcgatgagtg tgtccacctc tgcaatctca
      841 ttctcaatga ggaagcggaa gagacgggca gtgaggcgga tcatttcatc cccatggccg
      901 aagccccgaa gccgagggag ataaaggcct tcctcgacca gtatgtcatt ggccaggagt
      961 gggcgaaaaa gagcgtttcc gtagcggtct acaatcattt caagcgcatc agcaacaatc
     1021 tcgggaaaga tgccgacgtg gagctccaga aaagcaacgt gctgcttctc ggacctacag
     1081 ggtgcggcaa aacccttatc gcccagactc tggcgaaaaa actcaacgtt ccctttgcca
     1141 tcgccgatgc cactacgctt acagaggcgg gatatgtggg cgaggacgtg gagaatatcc
     1201 ttgtgaagct cctccaggct gccgattatg acgtgaaggc agcccagcgg ggaataatct
     1261 acatagacga aatcgacaag ctgtccagga aatcagagtc cgcatccatc acccgggatg
     1321 tttccgggga aggtgtgcag caggccttgc tgaagattct cgaggggacg gtttccaacg
     1381 ttcctcccaa gggcggccgg aagcacccgt accaggattt tatccagatc gacacgtcaa
     1441 acattctctt tatctgcggt ggagcctttg acggcgtgga ggagatcatc gggcgcaggg
     1501 tgaacaaaaa actcatcggt ttcggcggcg acatcatggg aaaacacgac gcccggagat
     1561 acgagatcat gaagcagata cagccggagg atctcatgac ctacgggttc attccggaac
     1621 tcgtcgggcg ccttccggtg cttgttccgc tcgaagaact ggacgccgac gcgcttgtcc
     1681 ggattctctc cgagccgaag aatgccctta tccggcagta ccagaagacc ttctccatgg
     1741 agggggtttc ccttgagttc actgatgaag ccgtcagaag catcgccatg aaggcgaacg
     1801 agaaaaacac cggagccagg gggcttcggt ccattcttga aaacctcatg atggaactga
     1861 tgtacgatat tccctccagg agcagggaag tccggaaggt catcatcacg aaagaggccg
     1921 tggaggggat gaaggatccc atccttgaag gaaagagctc caaggaggtc gcctgaccca
     1981 tggagagtac tcgccgctgc tacgtcctcc ccatacggga catcgtggtt ttccccggga
     2041 tcatagcccc cctttttgtg gggcgcccga gatccctgaa ggccatcgaa atggccatgc
     2101 tccaggacag aaacatcctc gttgtcgccc agaaggatat gcagattgac gatccccggg
     2161 tagaggatct ctacacagtg ggcactcttt gctctatcct gcagatggtg cgtattcccg
     2221 acggaacgac caaagtgctc atcgagggag tcgaaagggt cagggttgaa tccttcagca
     2281 ggggcaagga ggctctcgag gctgacgtgg tgccccttcc gtgggacgag agcctttctc
     2341 cgaacctcga ggccttgaag cggagtgtcc tcgaacagtt tgacaagtat gtgaccctcc
     2401 acccgaggat tccggcggag gtcctcgtca cggtgatgaa cgtcgatgac ccgaggcaga
     2461 tttccgacct tgtcagctcg cacctgtcca tcaaggttga acggaaacag cggctgctcg
     2521 aaatgattga tcccgaaaaa agcctcaagt tcatcctgaa gctccttctc gaggaaattg
     2581 acattctcca gctggagcat gacatccagg acaaggtacg ccaggaagtg gagcggggac
     2641 acaaggaata ctatctcagg gaacagttga agatcatcca ggacgaactc ggccagaacg
     2701 aggcaccgtc ggagatcgag gagctccgtg ggaagataga agaagcggac atgcccgagg
     2761 tttcgagaaa caaggccctc cacgagcttg accggctctc gaaaatgccg gtcatgtccg
     2821 ctgaagccac ggtggtcagg acctacattg actggctggt ggcccttccg tggagcaggt
     2881 tctcccctga caacatcgac atcgtcaggg ctgcaaaagt cctggaggaa gaccattacg
     2941 gcctggaaaa ggtcaaggaa cggatcctcg agttcctcgc cgttcggaag cttgcctcga
     3001 acaggatgaa aggacaggtg ctctgtttcg tcggtcctcc tggggtggga aaaacctccc
     3061 tcgggaaatc catcgcccgc gctcttgaaa gaaaattcgt cagcatgtcc ctcggcggca
     3121 tgagggacga ggcagagata cggggtcacc gcagaaccta cgtcggtgcc atgccggggc
     3181 ggatcataca aaaaatccgg caggccgggg taaggaatcc ggtgctgctc atggacgaga
     3241 tcgacaagat ggggcaggac ttcaggggag acccctccgc cgccctgctc gaggtgctgg
     3301 acccggaaca gaacggcgct ttcaccgatc atttcatgga ggtgccggtg gacctcagcg
     3361 acgtgatctt catcaccacc gccaacgtga cccatacaat tccccgtccg ctgctcgaca
     3421 ggatggaagt catcaaaata ccggggtacg tgtggcagga aaagaccagg atcgctaaga
     3481 gacacctgtt tccccgaatt cttacggagc acggcctgtc gccgaagcag gtgaaggtga
     3541 cccctaaggg tttggaacag ctgataagcg aatataccag ggaagcgggc gtcag
//
LOCUS       DAOTHD010000099         3594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_30679_length_3594_cov_2.691438, whole genome shotgun sequence.
ACCESSION   DAOTHD010000099 DAOTHD010000000
VERSION     DAOTHD010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30679_length_3594_cov_2.691438"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..487)
                     /locus_tag="P5219_02615"
     CDS             complement(2..487)
                     /locus_tag="P5219_02615"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HRX25681.1"
                     /translation="MDALDWMIVGELQENLPPGENPWQALACRLGISEDDVVARLKGL
                     KASGKLKRVGAVLRHQNSGFAANAMAVFLPPPGRTDELGEKLAASPMVSHCYERVPCG
                     EWIYTLYAMIHGRAEEEITAFVSAFAAENGVERYDILFSREELKKSSMVFFGRPGNEP
                     G"
     gene            complement(493..969)
                     /locus_tag="P5219_02620"
     CDS             complement(493..969)
                     /locus_tag="P5219_02620"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRX25682.1"
                     /translation="MVLPEMTMDPVGRKLVNLLQEGLPLEKDPYGILARELGVSREEV
                     TERIGALVDGGVIRRIGGVFDSAAMGYTSLLVGAAVPGEVFRDAAAFVNSFGGVTHNY
                     RRSGPLNMWFTFASGDPGEIDGLLAALRERFGLTSVYPFPKVKHFKLRVFFDMEKE"
     gene            complement(951..1937)
                     /locus_tag="P5219_02625"
     CDS             complement(951..1937)
                     /locus_tag="P5219_02625"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRX25683.1"
                     /translation="MLISWNTTSRCNLFCRHCYRESGPENDRSGELTTAEGRELIASI
                     RRAGFRLLIFSGGEPLLREDIFDLVTAARDAGLLPAMGTNGTLLTVETAARLAESGLR
                     GVAVSVDSLDREYHDTFRGTPGAFDRAQQGIRNAIEAGLRVQINLTLTDRNLDQFDRM
                     VDYYETMGVHAVHPFFLVPTGRALSMAEEELTSGAYFSMIRTVLEKQGSASLEIKPTC
                     APQFMPMAKEMGIPQRFTRGCLAGTAYCCVLPDGDVHVCPYLPVRAGSVRERPFEDIW
                     RDSEVFLKLRDRSAYGGSCGECGHWEICGGCRARAYHYTGNYLAEEPWCFRR"
     gene            complement(1948..3114)
                     /locus_tag="P5219_02630"
     CDS             complement(1948..3114)
                     /locus_tag="P5219_02630"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRX25684.1"
                     /translation="MISLTKLLLDREHFGDRLRYHGGAPAPAGAAPGLGPVVVWNCTR
                     ACNLACRHCYASATPGKSANELSTEEGRAFLESLAAFRVPAVLLSGGEPLMREDVFHL
                     LEYGASLGLRMVLSTNGTLIGSATAQRLRSAGVSYAGISLDGLEAVNDAFRGTKGAFE
                     KTLAAFRHCRAAGQKAGLRLSLTRTTVRELPAIFRLVEEEGISRVCLYHLVSAGRGSE
                     LQGEDLTAEETRDALDFLIEKTLDFGARGVDTEILTVDNHCDGLYVFLKMRERDPERA
                     ERIFGLLGRSGGNRSGMAIGAVDWEGNVTIDQFTRSIILGNIREKGFSDIWRGEGDSF
                     LAMVRDRKAHLGGRCGACRRQEQCNGNLRARALAAGDFWGPDPACYLTDGEIGL"
     gene            complement(3268..>3594)
                     /locus_tag="P5219_02635"
     CDS             complement(3268..>3594)
                     /locus_tag="P5219_02635"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRX25685.1"
                     /translation="GTPVGTCGFHNWKKEHGRAELGYELDSRMWRQGYMSEAVSAALE
                     YAFDTMALNRIEALVTAGNERSRRFLEKTGFTLEGTLRQYELARGSHQDQWMFSLLRE
                     ERAGRS"
BASE COUNT          620 a         1195 c         1110 g          669 t
ORIGIN      
        1 ctcagccggg ttcgttgccc gggcggccga aaaaaaccat gctgctcttt ttgagctctt
       61 ccctgctgaa gaggatatcg taccgctcca cgccgttttc ggcggcaaag gccgatacga
      121 aagctgtaat ttcctcctcg gccctgccgt ggatcatggc gtagagggta tagatccact
      181 ccccgcaggg gacccgctcg tagcagtggc tcaccatggg actggcggcc agtttttcgc
      241 ccagttcgtc cgtcctgccc gggggcggaa ggaagaccgc catggcgttg gcggcaaagc
      301 cggaattctg gtgcctcagg acggcaccga ccctcttgag ctttcccgac gctttcagcc
      361 ccttcagccg ggccaccaca tcgtcctcgg aaattccgag tctgcaggcg agggcctgcc
      421 agggattttc gccgggaggg aggttttcct gcagttcgcc gacaatcatc cagtcgagag
      481 cgtccacggc ggtcactcct tttccatgtc gaaaaagacc ctgagcttga agtgcttcac
      541 cttcgggaag gggtacacgg aggtaaggcc gaacctctcc ctgagggccg ccagcagccc
      601 gtcgatttcc ccggggtctc cggaggcaaa ggtgaaccac atgttcagcg gtccggagcg
      661 gcggtagttg tgggttacac ccccgaagga gttcacgaag gccgccgcgt cgcggaatac
      721 ctccccggga acggccgctc ccacgagaag gctcgtatac cccatggcag cggaatcgaa
      781 gacgccgccg atccgccgga tgacgccccc gtcgacgagg gcgccgatcc gctccgtcac
      841 ctcctcccgg gatacgccga gctcccgggc gagtatcccg taggggtctt tctccagggg
      901 gagtccctcc tggagaagat tgacgagctt ccggcccaca ggatccatgg tcatctccgg
      961 aagcaccatg gctcctccgc aagatagttt ccggtgtaat ggtaagccct ggccctgcac
     1021 ccgccgcaga tctcccagtg tccgcactcc ccgcagctcc ccccgtaggc cgacctgtcc
     1081 cgcagcttca gaaacacctc gctgtccctc cagatgtcct caaagggcct ctctctcacg
     1141 ctgcccgccc tgacgggcaa atatgggcag acgtggacgt caccgtcggg gaggacgcag
     1201 cagtaggccg taccggccag gcatccccgg gtgaacctct ggggtatgcc catctccttc
     1261 gccatgggca tgaactgggg ggcgcaggtg ggcttgattt ccagagaggc ggagccctgc
     1321 ttttcgagga ccgtccggat catggaaaag taggcccctg aggtgagttc ctcctctgcc
     1381 atggagaggg ctcttcccgt gggaacgaga aagaagggat ggaccgcgtg gacacccatt
     1441 gtttcatagt agtccaccat gcggtcgaac tggtccaggt tcctgtcggt gagggtgagg
     1501 ttgatctgca cccggagccc cgcctcgatg gcgttccgga tcccctgctg ggctctgtcg
     1561 aaggcccccg gggttccccg gaaggtatcg tgatattccc ggtccagact gtccacgctc
     1621 acggccactc cccgaagccc gctctccgcc agccgggcgg ccgtctccac ggtgaggagg
     1681 gtaccgttcg tccccatggc gggcagaagt ccggcatccc gtgcggctgt gacgaggtcg
     1741 aagatgtcct cccggaggag gggttcgcct ccgctgaaga tgaggaggcg gaaccccgcc
     1801 ctccggatgg aggcgatgag ttccctgcct tccgccgtgg tcagctcccc ggaccggtcg
     1861 ttttcagggc cggactcccg gtagcagtgg cggcagaaca ggttgcagcg gcttgtggta
     1921 ttccatgaaa tgagcattcc gcccctccta cagtccgatt tctccgtcgg tgagataaca
     1981 ggccggatcc ggcccccaga agtcccccgc ggccagggcc ctggccctga gatttccgtt
     2041 gcactgctcc tgcctccggc aggcgccgca ccgtcccccg aggtgggctt tcctgtcccg
     2101 caccatggcg aggaaggagt ccccttcccc cctccagatg tcggaaaatc ctttctcgcg
     2161 gatgtttccg agaatgatgg accgggtaaa ctgatcgatg gtcacgttgc cctcccagtc
     2221 cacggccccg atggccatgc ctgaacggtt gcctccgctc cggccgagga gcccgaaaat
     2281 ccgttccgcc cgctccggat ccctctcccg catcttcagg aagacataga gaccgtcaca
     2341 gtggttgtcc accgtgagga tttccgtgtc caccccgcga gcgccgaaat cgagggtctt
     2401 ttcgatgagg aagtccaggg cgtccctggt ttcttcggcg gtgagatcct ccccctggag
     2461 ctcgctcccc cttccggcgg acaccaggtg gtaaaggcag acccgggaga ttccctcctc
     2521 ttccacgaga cggaagatgg cgggaagctc ccgcaccgtg gtccgggtga gggagagccg
     2581 gagccccgcc ttctgcccgg cggcccggca gtgccggaag gccgcaaggg ttttttcgaa
     2641 ggcgcccttc gtgccccgaa aagcgtcgtt tactgcttcc agaccgtcaa ggctgatgcc
     2701 ggcgtagctc acgcccgcgg accggagcct ctgcgccgtg gcgctgccga tgagggtgcc
     2761 gttggtggag aggaccatcc tgagccccag ggaagcgccg tattccagga gatggaagac
     2821 atcctccctc atgaggggtt ccccgccgga aaggaggact gcgggaaccc ggaacgccgc
     2881 cagggattcc aggaatgccc tgccttcttc cgtggaaagt tcgtttgccg atttgccggg
     2941 cgtcgccgaa gcgtagcagt ggcggcacgc caggttgcag gccctggtgc agttccacac
     3001 caccaccggt cccaagccgg gggcggcccc tgcgggggcc ggagcgcctc cgtggtacct
     3061 gagcctgtct ccgaaatgct cccggtcaag cagcagtttt gtcaggctga tcaagtaaat
     3121 acctcctccg ggcaagggca gcggtcagcg aagatgcggg gccgcgcccg aagagcatgt
     3181 gaggccccgg ctgaacggaa tggggcgggc tatttcactt cgtcgagggg aatcccgagg
     3241 accgcctgcc gtatgagttt gttcgctcta cgaacgtcct gcgcgctctt cccgaagaag
     3301 ggagaacatc cactggtcct ggtggctccc gcgcgccagt tcatactgac gcagcgtccc
     3361 ttcaagggtg aaaccggtct tctccagaaa acggcgcgag cgctcgttcc cggccgtcac
     3421 cagggcctcg atccggttca gcgccatcgt gtcgaaggca tactccagcg cggccgagac
     3481 ggcctcgctc atgtacccct gccgccacat ccgtgaatcg agttcgtacc caagctcggc
     3541 acgcccgtgt tccttcttcc agttatgaaa accgcacgtg ccgaccggcg ttcc
//
LOCUS       DAOTHD010000100         3561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_31060_length_3561_cov_3.614661, whole genome shotgun sequence.
ACCESSION   DAOTHD010000100 DAOTHD010000000
VERSION     DAOTHD010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31060_length_3561_cov_3.614661"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="P5219_02640"
     CDS             complement(<1..154)
                     /locus_tag="P5219_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HRX25686.1"
                     /translation="MAELTVFGGGSWGTALAASAAASGHSVCLWCRRSEQARAITATG
                     KNPDYLR"
     gene            complement(147..1334)
                     /locus_tag="P5219_02645"
     CDS             complement(147..1334)
                     /locus_tag="P5219_02645"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRX25687.1"
                     /translation="MILALILGWAVLYADRTALYPLLSVIADSLGISSASAGAITSTY
                     FLLYVLPQIPSGFAADRWGAKRVLIFMFALSGLGILGFGLFGTSYALLLFFSGLHGFG
                     ASAYYPCCFGTMLSTVPAEKRGLSSGLIGIGMAVGTIGGMAVSGPLYQFFGSYRIPFI
                     LLAVPSVLVIFMFMKYLPDVRNDVSPSFAAYGRLFRDRDIWKINLATFTSLYGFWAAA
                     TWGPTFLQAERGFSLTQSGLYTGLIALTALPGGILWGRLSDRLGRKRTALFVLPLSAA
                     ALFLLTRAQGTPAIVGTLLLFGLFSNTAFTPIAISWVGDIVSRRHPGSMGAAVGFFNG
                     SVMSSAVVAPLVSGFLRDLTGSLVPAITAGVALMFAGSALLLFTPATPDGKDGKGKEV
                     HNG"
     gene            1476..2615
                     /locus_tag="P5219_02650"
     CDS             1476..2615
                     /locus_tag="P5219_02650"
                     /inference="COORDINATES: protein motif:HMM:NF017221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRX25688.1"
                     /translation="MAPDDMAMRLKEIQDKVSESLQYSIDSLVTLREEERERLFEIRT
                     RREGVQKELNEVIVASEKAEAEYRRSRQVLLDASRSGNESREKEAYERAMNLMKIRGA
                     FEEREKHLAAQRDDLDREERRIERLIARSEEMGNRFRVVLNLLNFSIEDEGSGTLSPE
                     QKEFSAGLLLAERESISLSRELHDGPIQKFSAAGLMIDLAGEFLSRGDFGKAKEELAR
                     TRSHIGDALEEFRSFLFQLNPTGLKEGFDVALSRLVAQTSSLSGADVRYAVEGQSDLI
                     SLPMRTTVFKIIQQAVVNAVKNGKARRIRVLVSIGREMLRVKIVDDGLGFDVEKVRSE
                     AEEKGAWGLVNMEDRAAMIGGEFSVLSEPGKGATVSLAVPLPVKR"
     gene            complement(2652..>3561)
                     /locus_tag="P5219_02655"
     CDS             complement(2652..>3561)
                     /locus_tag="P5219_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRX25689.1"
                     /translation="EPTVSMFFLVNNGPFSGQEGQAITLRQLKARLQREMHVNVALRM
                     EDLGRPDGVKVSGRGELQLAILIEEMRREGMEFCVSKPEVITEEIGGVLHEPMEDLVV
                     DIPEEYQGIVFEKLSRRKARVKEIQNLRTGLMRIFFNIPTRGLIGYRGEFLTDTRGLG
                     IMSSRFSGYSPWSGEITPRSRGALVSLDTGEATSYQLENLQERGTLFVSPMDRIYTGM
                     IVGENSRPNDMPCNPTKRKQATNHRSSTKDFTVKLDVPRKMGLEKALEWISEDELVEV
                     TPKSIRLRKIILDDGERRKAAKKLSA"
BASE COUNT          734 a         1047 c         1146 g          634 t
ORIGIN      
        1 cacggagata gtccgggttt tttcccgtgg cggtgattgc tctcgcctgc tcgcttcttc
       61 tgcaccaaag gcagactgaa tgtcccgaag ccgccgccga tgccgccagg gcggtgcccc
      121 agcttcctcc gccgaaaacc gtcagttcag ccattgtgta cctcctttcc cttcccgtcc
      181 tttccgtccg gtgtggccgg cgtgaacaga agcagcgccg agccggcgaa catgagcgct
      241 acgccggcgg ttatggcggg aacgagggac cccgtaaggt cccgaagaaa tcccgaaacc
      301 aggggggcca ccacggcgga cgacatgacc gagccgttga aaaagcccac cgcagccccc
      361 atggaaccgg ggtgccgcct gctgacgata tcgcccaccc acgagatggc gatgggggta
      421 aaagccgtgt tgctgaagag cccgaagaga agcagcgtcc cgacgatggc aggagttccc
      481 tgggcccttg tgagaaggaa aagggccgcg gcgctcagcg ggaggacgaa gagagccgtc
      541 cgtttcctgc ccagacggtc ggagagccga ccccagagaa tgcctcccgg gagagccgtc
      601 aatgcgatga ggccggtgta gagcccggac tgggtcaggg agaacccccg ctccgcctgg
      661 aggaacgttg ggccccaggt ggccgcagcc cagaagccat agagggacgt gaatgtggcc
      721 aggttgatct tccagatgtc cctgtcgcgg aacagcctgc cgtaggccgc aaaggacggg
      781 gagacgtcat ttcggacgtc ggggaggtac ttcatgaaca tgaagattac aaggacggag
      841 ggaactgcca ggagaatgaa ggggatcctg tagctgccga agaactggta gagggggccg
      901 ctgaccgcca tccccccgat ggtgcccacg gccatgccga ttccgatgag acccgagctg
      961 agcccccgct tttccgccgg gacggtggag agcatggttc cgaagcagca ggggtagtag
     1021 gcggaagctc cgaagccgtg gagccccgag aaaaagagca ggagtgcgta ggatgtgccg
     1081 aagagcccga atccgaggat tccgaggcct gaaagggcaa acatgaaaat caggactcgt
     1141 ttcgcacccc acctgtcggc ggcgaatccg ctggggatct ggggcaggac gtacagcagg
     1201 aaataggtgc tggttatggc accggcgctt gcggatgata tgccgagact gtcggcgata
     1261 acggaaagaa gagggtacag ggctgtcctg tctgcgtaca gaacggccca gccaagtata
     1321 agagccagga tcacggtata ttcctcccag tcaggttgtg tagcgtctat tgtatccctt
     1381 ttcgccggga aggaagagcg ggaaattgcc agacttcatc gggagggcta caatacatca
     1441 ggtaatgcat ccgctcctga cgggaggaat ctggaatggc gccggacgac atggccatga
     1501 ggctgaagga aatccaggac aaggtgagcg aatcactgca atacagcatc gacagcctcg
     1561 ttacgctccg ggaggaggag cgggagcgcc ttttcgaaat ccggacccgg cgggagggcg
     1621 tgcagaaaga actcaacgag gtcatcgtgg cgtcggaaaa agccgaggcg gagtacaggc
     1681 gatccaggca ggtcctcctg gatgcctcca ggtccgggaa cgagtccagg gaaaaagaag
     1741 cctacgagcg ggccatgaac ctcatgaaaa tcaggggggc cttcgaggag agagaaaaac
     1801 acctggccgc ccagcgggac gatcttgacc gggaggagcg ccgcatcgaa cggctcatcg
     1861 cccggagcga ggagatgggc aaccggttca gggttgtgct caatctgctc aacttcagca
     1921 tcgaggatga aggatcgggt accctttctc ccgagcaaaa agagttcagc gctgggctcc
     1981 tgctggcgga gcgggaaagc atatccctct cccgggagct ccatgacggc ccgattcaga
     2041 aattttccgc cgccggcctg atgatcgatc tcgccggaga attcctctcc cggggggatt
     2101 tcgggaaggc gaaggaagaa ctcgccagaa cccgttccca catcggggat gccctggagg
     2161 aattccgctc gttcctcttc cagctcaacc ccaccggcct caaggagggg ttcgatgtcg
     2221 ccctgagccg cctcgtcgcc caaacctcct ccctgtccgg agcggacgtg cgctatgccg
     2281 tggaaggtca gtccgacctg atttccctcc ccatgcggac caccgtgttc aagatcatcc
     2341 agcaggcggt ggtcaacgcc gtgaaaaacg gaaaggcacg ccgcatcagg gttcttgtga
     2401 gcataggcag ggaaatgctg cgggtgaaga tcgtcgatga cgggctcggc ttcgacgttg
     2461 aaaaagtgcg gagcgaggcc gaggaaaaag gcgcctgggg gctggtcaac atggaagacc
     2521 gggccgccat gatagggggg gagttctccg tcctcagcga acccggaaaa ggagccacgg
     2581 tctccctcgc ggttcccctc cccgtgaaac gataggcaca gggccgcccc ggttgggagg
     2641 cggccctttt tttatgcgga cagctttttc gcggcttttc tccgctcgcc gtcgtcgagg
     2701 atgatcttcc tcaggcggat ggacttcgga gtcacttcca ccagctcgtc ctcggaaatc
     2761 cactccagcg ccttttcgag ccccattttg cgcggcacgt cgagcttcac cgtgaagtcc
     2821 ttcgtggagg accggtggtt cgtggcctgc ttccgcttcg tggggttgca gggcatgtcg
     2881 ttgggacggg aattctcgcc tacgatcatg ccggtgtaga ttctgtccat gggagagacg
     2941 aacagggtcc ccctctcctg gaggttctcg agctggtagc tcgtggcctc tccggtgtcg
     3001 aggctcacaa gggctcccct gctgcggggg gttatctccc cggaccacgg ggagtacccc
     3061 gagaagcggg aggacatgat gcccaggccc ctcgtgtcgg tgaggaactc gccgcggtag
     3121 ccgatgaggc cccttgtcgg gatattgaag aatatgcgca tgagcccggt gcggaggttc
     3181 tggatctcct tcaccctggc tttccgccgg gacagttttt caaagacgat gccctggtat
     3241 tcctccggga tatccaccac caggtcctcc atgggctcgt gaagcactcc gccgatctcc
     3301 tcggtgatca cttccggctt ggagacgcag aactccatcc cttcccggcg catctcttcg
     3361 atgagaatgg ccagctgcag ctctccccgg ccggagacct tcacgccgtc cggccgtccc
     3421 agatcttcca tccgaagggc cacgttgacg tgcatttccc tctgcagcct ggccttgagc
     3481 tgccggaggg tgatggcctg tccctcctgg cccgagaagg ggccgttgtt cacaaggaag
     3541 aacatcgaga ccgtcggctc c
//
LOCUS       DAOTHD010000101         3555 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_31136_length_3555_cov_3.347714, whole genome shotgun sequence.
ACCESSION   DAOTHD010000101 DAOTHD010000000
VERSION     DAOTHD010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31136_length_3555_cov_3.347714"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="P5219_02660"
     CDS             <1..319
                     /locus_tag="P5219_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRX25690.1"
                     /translation="DLMDDEQDCIRWRDLNQMFHNLLFEPADRPLTLDMLARLRQKTD
                     RNIRIHLASMREESQRQHRAILAAVAGRNLSAALEALADHLTYTSNDLQSCMRLEQGN
                     KKR"
     gene            565..1872
                     /locus_tag="P5219_02665"
     CDS             565..1872
                     /locus_tag="P5219_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRX25691.1"
                     /translation="MSFLNNVLAIFILILVSAFFAVSEISLAASRKIKLKLLLKKGDH
                     RAQRVLDLQENPGHYFTVIQIGLNAVAILGGVMGESALSPYISSWLHHLYDGPALQTV
                     SFAVSFSFVTGAFVLFADLIPKRTGMIEPERIAMAVAAPMTVCMKLFAPLVWFFNGLA
                     DMLFRLFRVPIVRREDITSDDVMAMADAGAQAGVFLNKEQHLIANVFELDTRGVASAM
                     SSRNDIVFLTLGESEESLYAKITERPHGKYPVCERDSIDTVMGYVDSKDLLPRIAKGS
                     KISLRTDPIVRKILILPDTLTLFEALERFRDAREDMAVIVNEYALVVGLLTLQDVMST
                     VMGELISPFQEELIVQRDENSWLVDGTTPIEDVMQALDIDEFEGWENYETIAGFIMYM
                     LRRVPKRTDKVEYGDHAFEVVDIDNYRIDQVLVTRIGGEGRTA"
     gene            complement(1957..>3555)
                     /locus_tag="P5219_02670"
     CDS             complement(1957..>3555)
                     /locus_tag="P5219_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25692.1"
                     /translation="PHILDELESFCVSERKHPVKVVVMGETPPSPSGWPLVPKPHVRV
                     ITRTGSFQNEDALERISAHRASAVIVLGESSSDRKLSERLNDPIVTRTLLALETLLGD
                     RRKRGWTQGWKSDRSPVIILNYLDLGNSLHVTDFLRPFGSENTRIFFNPLFFTGRLIS
                     SMCLNPYAEDIFNELMTAEGNEFHYVSPSIGRGMIWRDIAGAFPKSVPVGYRDGNGEL
                     RMVPFPDEPLPEDAGIVVLSENGWDAALFDPSAPGGRQDGKGPSLFCPMGGLPSGTLV
                     IVGVNPKLPFIIEGLGRLAMKITIADNQTEEEFAAWYAEYSRTPLPGGVFFQECRFRS
                     EEEVRRAIDIGGTDRIILLADGHLLNAATPDRIDAETVSKLLMLSRLIGQEKREDIRL
                     IVETLTVDSEVVVRNIRNCSNVIGPLTIGRLLTTFALQPEFEPVFRSLIQFGDIDIVC
                     CPAEAALSGNPEGAPLSFGDLLSMPRNGSIPIGWVVPPPGSEDGRKTDPGGLRRLAPT
                     VMLNPPKNSLLPPGAEIIFLHREA"
BASE COUNT          682 a         1095 c         1065 g          713 t
ORIGIN      
        1 cgatctcatg gacgatgagc aggactgcat ccgctggcgg gacctcaacc agatgttcca
       61 taacctgctc ttcgagcccg ccgaccgtcc cctcaccctg gacatgctcg ccaggctccg
      121 ccagaaaaca gaccggaaca tccgcatcca cctcgcctcc atgcgtgagg agtcccagcg
      181 gcagcaccgg gccatcctcg ccgccgtggc cggacggaat ctgtccgctg ccctggaagc
      241 tctcgccgat cacctgacct acacgtccaa cgacctccag tcctgcatga ggctcgaaca
      301 ggggaacaaa aaacgatagc tggtccggaa agggcgcatt gtcccctgcc ctggaaagcc
      361 cgcggggtat cttcacccgg agaaaaatcc gttgtttctg ctgcggagaa agtgcctcca
      421 tgccgtctga ctgcgctgtt ttccccggga tcgtgtaaaa tagaacgtat tcgtccctga
      481 ggtcagaatt gtccgacctt ttgtgtgtcc tgtgttttga accgatcttc ctgagtgtgg
      541 gattcatccc cggaggtacc agcgatgtct tttctcaaca acgtgctcgc gatttttatc
      601 ctgattcttg tcagcgcctt ctttgccgta tcggagattt ccctcgccgc atcgcggaaa
      661 atcaagctca agctcctgct gaagaaaggc gaccaccggg cccagagagt gcttgacctg
      721 caggagaacc cgggccatta tttcaccgtc atccagatcg gcctgaacgc tgtggccatc
      781 ctgggcggcg tcatgggcga gtccgccctt tccccataca tttcttcctg gctgcaccac
      841 ctgtatgacg gtcccgcgct ccagacggtt tcctttgccg tttccttctc tttcgtgacg
      901 ggggctttcg tcctttttgc cgatcttatc ccgaaaagaa ccgggatgat cgagcccgaa
      961 cggatcgcca tggcggttgc cgctcccatg accgtctgca tgaagctctt cgctcccctg
     1021 gtgtggttct tcaacggtct tgcggacatg ctgttcaggc tgttccgcgt tcccatcgtc
     1081 cggcgggagg acatcacgtc cgacgacgtc atggccatgg ccgacgcggg agcccaggcg
     1141 ggggtgttcc tgaacaagga gcagcacctc atcgccaacg tcttcgagct ggacacgaga
     1201 ggggttgcct cggccatgtc gtcgaggaac gacatcgttt tcctcaccct gggcgagtcc
     1261 gaagagagcc tctacgcaaa gataacggag aggccccacg gaaagtatcc cgtctgcgag
     1321 cgggattcca tcgacacggt catggggtac gtggactcca aggatctcct ccccaggatc
     1381 gcgaagggga gcaagatctc cctgcggacc gaccccatcg tccgcaagat cctcatcctg
     1441 cccgatacgc tgactctttt cgaggccctc gagcggttcc gcgacgccag ggaagacatg
     1501 gccgtcatcg tgaacgaata tgccctggtc gtggggctgc tgacgcttca ggatgtgatg
     1561 agcaccgtca tgggcgagct catcagtccc ttccaggaag agctcatcgt gcagcgggac
     1621 gagaattcct ggctggtgga cggcaccacc cccatagagg acgtgatgca ggccctggat
     1681 atcgacgagt tcgaggggtg ggagaattat gagaccatcg ccggattcat catgtacatg
     1741 ctcaggaggg ttcccaagcg gaccgacaag gtagagtacg gcgaccatgc ctttgaagtt
     1801 gtggatatcg acaactaccg gatcgaccag gtgctggtca cccgcatcgg cggggaaggc
     1861 aggaccgcgt aattccgcag gcttacagtt cggaagaaaa tgacatcccc gggggcacag
     1921 tgcctccggg gatgtttttt cgttcggtgt ccgatctcat gcctcccgat ggaggaagat
     1981 gatctccgcc ccgggaggaa gaaggctgtt cttgggcggg ttcagcatga ccgtcggtgc
     2041 gaggcgtctc agccctcccg ggtcggtttt cctcccgtct tcgctcccgg gcgggggaac
     2101 cacccagccg atgggaatgg agccgttccg gggcatgctg agaagatccc cgaaggaaag
     2161 gggcgctcct tccgggtttc cggaaagggc ggcttccgct ggacagcaga cgatgtcgat
     2221 gtcgccgaac tggatgaggg aacggaagac aggctcgaat tcaggctgaa gggcgaaggt
     2281 ggtcagcagc cttccgatgg tcagggggcc gatcacgttg gagcagttgc ggatgttccg
     2341 caccaccact tccgaatcca cggtcagggt ctccacgatc aggcggatat cttcccgctt
     2401 ctcctgcccg atcagcctgc tgagcatcag gagctttgac actgtttccg cgtcgatccg
     2461 gtccggcgtg gccgcgttca gcaaatgtcc gtccgccagg agaatgatcc tgtccgttcc
     2521 gccgatgtcg atcgcccggc ggacttcctc ctcggaccgg aagcggcatt cctggaaaaa
     2581 cacgccgccg ggaaggggcg tcctggaata ttcggcgtac cacgccgcga actcctcttc
     2641 cgtctggttg tccgcgatgg tgatcttcat ggcgagccgg cccagtcctt cgatgataaa
     2701 gggaagcttc gggttcaccc ccacgatgac cagggtcccc gaaggaaggc cgcccatggg
     2761 gcagaacagg gaaggcccct tcccatcctg ccgtcccccc ggcgccgaag gatcgaaaag
     2821 ggcggcgtcc cagccgtttt cgctcaggac gactatcccg gcatcttccg gaagaggttc
     2881 atcggggaag ggaaccattc gcagctcacc gttcccgtcc cggtacccca ccgggacgga
     2941 cttcgggaag gccccggcga tgtccctcca gatcatcccc cggccgatgg acgggctgac
     3001 gtaatgaaac tcgttcccct ccgccgtcat gagttcgttg aaaatatcct ccgcgtaggg
     3061 gttgaggcac atggacgaga tcagcctgcc ggtgaaaaag agggggttga agaagatcct
     3121 cgtgttctcc gaaccgaagg ggcggaggaa atcggtcaca tgaaggctgt tccccaggtc
     3181 gagatagttc aggatgatca ccggcgacct gtcggacttc catccctggg tccatccccg
     3241 cttccttctg tcgcccagga gcgtctccag ggcgaggagg gtccgggtga cgatggggtc
     3301 gttcagcctc tcggacagct ttcggtcgga agagctctcc ccgagaacga tcaccgccga
     3361 ggcccggtgg gccgaaatgc gttccagggc atcctcgttc tggaagctgc ccgtcctggt
     3421 gatgaccctg acgtgaggtt tcggcaccag gggccagccg gagggcgaag gtggcgtctc
     3481 ccccatcacc accaccttca cggggtgttt ccgctcggaa acacagaagg attcaagctc
     3541 gtccaggata tgggg
//
LOCUS       DAOTHD010000102         3554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_31150_length_3554_cov_2.971706, whole genome shotgun sequence.
ACCESSION   DAOTHD010000102 DAOTHD010000000
VERSION     DAOTHD010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31150_length_3554_cov_2.971706"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..81)
                     /locus_tag="P5219_02675"
     tRNA            complement(5..81)
                     /locus_tag="P5219_02675"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45..47),aa:Met,seq:cat)
     gene            complement(95..181)
                     /locus_tag="P5219_02680"
     tRNA            complement(95..181)
                     /locus_tag="P5219_02680"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Leu,seq:taa)
     gene            complement(281..1504)
                     /locus_tag="P5219_02685"
     CDS             complement(281..1504)
                     /locus_tag="P5219_02685"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRX25693.1"
                     /translation="MNSVKLIPVDLLFDEEGTIAADVVSSSSALLLPAGLHLQSLRKT
                     HPGALDLLRKHGVTQIPIKKAVLITIDEFRNLLHRVHPPVSRLNPLAAQVAAHQMEVV
                     YSHIRNKAVREKGIRSLLTLGNTLCRQVKKTPQVTLSLGEGAERKQTPYLHGINVSLL
                     AGHIAGRLFPLWTGFVESVTVAGLLHDIGKSFLFPFAGERGRKSAISDSQAIHTHTLL
                     GEALLRDAGVTAQDILSAVRSHHESWDGTGYPDGLSGEAIPVGARIVGVANFFENLVS
                     AWDVRERKRSDQAVSSLLAAANGRFDKFIVRALLASVGLFPPGAVVELSDGRRGVVLE
                     SKERDLIRPRVLLVTGEEAEGDAIPAVIDLKKAGNVFIRQVFDDYGKMNIPPLRREGR
                     SGVLFRKYHAPSSMG"
     gene            1697..2722
                     /locus_tag="P5219_02690"
     CDS             1697..2722
                     /locus_tag="P5219_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRX25694.1"
                     /translation="MPLYLDKNENPFQTPKEIRDAVAEKISGLMFNRYPDPGYPSLKR
                     KLADLAGLSPESVVTGSGGDEILAMIFSRFVRPGDPVLTFSPTFSEYYRLSRMAGARH
                     ITLPIHMEGDEAVFPAGPFLEAVEREKPALVLLDTPNNPSGASLPSSFIGDVIARSES
                     FVVVDEAYGEFADSTFLAGLSGKGIPDRVIVLKTLSKAWGMAGIRFGWAYCGISAASP
                     LEEARSPFNVGIFQEAAAEIVLDHPEFPAISAQKVKKLRESVRQCINVLEGWKAFRSD
                     GNFLLLRIPFPEGMVRSAAEEDFCFKFLGLTPETGNRECWIRLTIGTEDEMAEVLRFF
                     SLLGKKS"
     gene            2752..3048
                     /locus_tag="P5219_02695"
     CDS             2752..3048
                     /locus_tag="P5219_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HRX25695.1"
                     /translation="MVEKWKDKLTDQLCQALLSLETPEEIYSFLEDVATIGEIRALAQ
                     RLEVSRLLSEGYTYPQIAQQTGASTATISRVKKFLEYGADGYKIVLERLRKEES"
     gene            complement(3074..>3554)
                     /locus_tag="P5219_02700"
     CDS             complement(3074..>3554)
                     /locus_tag="P5219_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRX25696.1"
                     /translation="AAAASEDIAECIRPAGLARTKSERMLRILDMIRKSFGGYSLRDL
                     KSWEGAKVREYLSSLPGIGAKTIACVMLFDLGKPAFPVDTHIARFCRQMEWVPEKTAP
                     EDIQGLLEEWVPQERFYGGHINIIEHGRGICGARKPDCGRCLIAPLCPYNGKNGALS"
BASE COUNT          807 a          935 c          965 g          847 t
ORIGIN      
        1 aaaatggttg cgggggcagg atttgaacct gcgaccttcg ggtcatgagc ccgacgagct
       61 accgggctgc tccaccccgc gatattgtct ttcctggtgc cggacgcggg actcgaaccc
      121 gcacggacct tcgtccacag gattttaagt cccgtgcgtc tacccattcc gccagcccgg
      181 ccagcactag ggcattatag agcacccccc cttgtttcgt caagcagata tggaggtgtt
      241 ttttcgaccg aaggttcttt tctccataag aacgggaatt ttaccccatt gaactcggtg
      301 cgtggtattt ccggaacagg acgcctgacc gtccttctct tctcagtgga ggtatgttca
      361 tctttccgta atcgtcgaag acctggcgta tgaatacgtt tcccgccttt ttcagatcga
      421 tgacggccgg aatggcgtcg ccttcagctt cttctccggt caccagaagg actctcggtc
      481 gtatcagatc ccgttccttt gactcgagca ccactcccct gcgaccgtcg gacaattcaa
      541 ccaccgcgcc cggaggaaag agacccaccg acgccaggag agcccgtact ataaatttgt
      601 cgaaacggcc gtttgcagct gcaaggaggg atgatacggc ctggtcgctt cgtttccttt
      661 cccttacatc ccatgcggaa acaaggttct caaaaaagtt tgctacccct acgattctcg
      721 cccccaccgg aatggcttct ccgctgaggc cgtcggggta ccccgtacca tcccaggatt
      781 cgtggtggga acggactgcc gagagtatat cctgtgccgt gactccggcg tcccttaaaa
      841 gagcttctcc aagaagggtg tgtgtgtgga tggcctgact gtcggaaatg gcggatttcc
      901 tgccccgctc tcctgcaaag ggaaaaagga aggatttccc aatgtcgtgc agaaggccgg
      961 ctacagtaac ggattcgacg aatcctgtcc agagagggaa cagtctgccg gcaatgtgcc
     1021 ccgccagaag tgagacattg attccgtgaa gatagggtgt ctgtttcctt tcggcacctt
     1081 ccccgagaga aagggtgacc tggggggttt ttttcacctg ccggcagaga gtgtttccga
     1141 gtgtgagcag agaacggatt cccttttccc gaactgcctt gttccggatg tgggagtaca
     1201 ctacttccat ctggtgagct gcgacctggg ccgcaagggg attgagccgg gagacgggcg
     1261 ggtgtacgcg gtgaagaagg tttctaaatt cgtcgatggt gataagaacc gcttttttga
     1321 tcggaatttg ggtaacccca tgctttcgaa gcaggtcaag tgccccggga tgggtctttc
     1381 tgagcgactg aagatgaaga ccggcgggaa ggagcagtgc cgaggaagaa gaaaccacgt
     1441 ccgccgcaat ggttccttct tcatcgaaga gcaggtcgac cggtatgagt ttgacggaat
     1501 tcacttcctc tacctccgaa aaaagaatga ttacccccac tttttttctt ccctgcggga
     1561 atccgaactg cggaaggcgg gcatactgcc ttcctgcgag attgacattg acgcaccacc
     1621 atgctagact gcaaaagact ttaggaaatg cacaatgtaa tatgataaag ttttcaacac
     1681 tttaggagac ggcattatgc ccctttatct ggacaaaaac gaaaatcctt tccagacgcc
     1741 aaaggaaatc agggatgctg ttgcggaaaa gatttccggg cttatgttca acaggtatcc
     1801 tgaccccggg tatccatccc tgaagcgtaa gctcgccgac cttgccggcc tttctccgga
     1861 atccgtggtg accggcagcg gaggcgatga aatcctcgca atgattttct cacgttttgt
     1921 acgccccgga gacccggttc tgaccttttc ccccactttt tcggagtatt acaggctgtc
     1981 gcggatggca ggagcgagac atatcactct tcccatccac atggagggag atgaagccgt
     2041 ttttcccgcc ggaccgtttc tcgaagcggt tgagagggaa aaaccggccc tggtgcttct
     2101 cgatacgccg aataacccaa gcggagcaag tctgccttct tcttttatcg gagatgtgat
     2161 tgcccgaagt gagtcctttg tcgtggtcga tgaagcatac ggtgaattcg ctgattccac
     2221 tttcctggcc ggtctttcag gaaaggggat tcccgacaga gtcattgttt tgaaaacact
     2281 ctcaaaagca tggggaatgg ccggaatccg tttcggatgg gcgtactgcg gcatttctgc
     2341 cgcatcccca ctcgaagagg caagaagccc cttcaatgtc ggcattttcc aggaagccgc
     2401 cgcggaaatt gttcttgacc atccggagtt tcctgccatc tcagcacaaa aagtgaaaaa
     2461 actgcgtgaa tctgtccggc agtgcatcaa tgtccttgaa gggtggaagg cattcaggag
     2521 cgacggaaat ttccttctgt tgcggattcc ttttccggaa gggatggtcc gttctgctgc
     2581 tgaagaggat ttttgcttca aatttcttgg tctgacgccg gaaacaggaa acagggagtg
     2641 ctggatcagg ctgacgatcg ggacagagga tgaaatggct gaagttcttc gtttcttttc
     2701 tttactgggg aaaaaatcct gaacggttta gaagccgagg aggtgccatt tatggtcgaa
     2761 aaatggaagg ataaattaac ggatcagctc tgccaagcgc ttctctccct cgaaactccg
     2821 gaggaaattt acagtttcct cgaagacgtg gcgaccatag gggaaatcag ggccctggcc
     2881 cagcgtcttg aagtatcgcg cctcctgagt gaggggtata cataccccca gattgcccag
     2941 cagacgggtg caagcaccgc gacaatcagc cgggtgaaaa aattcctgga atacggagct
     3001 gacggctata aaatagtgct tgaaagactg cggaaagaag aatcctgata atcgtcggtg
     3061 aaaaacggga ccctcatgag agggccccgt tttttccgtt atatggacag agcggggcga
     3121 tgagacatct gccgcagtcc ggttttcttg caccgcagat gcctctgcca tgttcaatga
     3181 tattgatgtg ccccccgtaa aaacgctcct ggggaaccca ttcctcgagg agaccctgaa
     3241 tatcctccgg ggccgttttt tccggaaccc attccatctg cctgcagaac cttgcgatgt
     3301 gggtgtctac agggaaagcc ggtttcccga gatcgaacag catgacgcag gcgatggtct
     3361 ttgcgccgat tcccggaagg gaggaaaggt attcccttac cttcgcccct tcccaggatt
     3421 tgagatccct cagtgaataa ccgccaaagg atttcctgat catatccagg atgcggagca
     3481 ttctctccga ttttgttctt gccagcccgg cgggcctgat gcattcggcg atgtcttctg
     3541 aagcagccgc agcg
//
LOCUS       DAOTHD010000103         3550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_31205_length_3550_cov_3.607153, whole genome shotgun sequence.
ACCESSION   DAOTHD010000103 DAOTHD010000000
VERSION     DAOTHD010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31205_length_3550_cov_3.607153"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="P5219_02705"
     CDS             complement(<1..219)
                     /locus_tag="P5219_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25697.1"
                     /translation="MDLDRLRENIARLFLGRVSVRSWRPGVSVPETRYLKRKAAVRPL
                     RPPRRALGTVLYGAAMSTKIKGGPLPLHE"
     gene            complement(209..778)
                     /locus_tag="P5219_02710"
     CDS             complement(209..778)
                     /locus_tag="P5219_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25698.1"
                     /translation="MASQAAGKNIGYVLVDGTEGTFRGAVLVTDFRGIPADFRYTDPI
                     TPSKIEKILYGNALDVYIKEELILESLVGAVEVTPVLWVCREGDLLDPLAKLAKSKTI
                     LLAPTARSPLDATGDIEKQNDPGSYIVQADGVSAPLRFSLPGEKNREEDARSAASILV
                     EAAATMDLLEPFSRMGKAMSALGEEKSGS"
     gene            complement(806..1114)
                     /locus_tag="P5219_02715"
     CDS             complement(806..1114)
                     /locus_tag="P5219_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25699.1"
                     /translation="MNMQLAHWNLEHTAQQLRDPSAASLQAGRQGEGVAAAVHRDSTV
                     QAAEQSAEEERLGAKKKRDQSEKDGKRGKQTRSPRDDEEPDKDPPSPGADRGTLDFYA
                     "
     gene            complement(1148..2743)
                     /locus_tag="P5219_02720"
     CDS             complement(1148..2743)
                     /locus_tag="P5219_02720"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HRX25700.1"
                     /translation="MADDRFHLEFREDGVYLSASEGADYSMPSVVNFMKLKGVENYNG
                     DVVMTFLSRKDGSPVKVAERDPEQEKEAALEVKISSDAMSAELWIEPPFADKPWPTVG
                     QVLEFLSAQGVVEGVDKGLISDILSEKRGRSWTAVASGTPATDGADADFDYKVQFGAA
                     KPKEEDESGRVDLKNLSSVTVVMKNQMLAEKIPATEGTDGITVKGTVLKAKKGKDRPL
                     PAGAGTYASEDGIHLLSLIDGNLVLKGGKLHVVPVFQVDGDVDYSVGNINFIGAVLVN
                     GAVREGFEIVSSGDIEIRGVVEGAHLSSTGNISITGGIRGMNKAKIEADGNITAGFID
                     QAHVRSGQSITVTNAVLHSDLAAHGNITVLGGQKSQIAGGKVQAGLEVVCLTLGSEMG
                     TKTEVVVGVLAELTERKKQLLALLAESEEKGEKIEANLLFLKKLEGAGQLDENKRALM
                     ISLTKAKFQLQSQLGTAREELRIIEEQMENSKAKGCVRVKGICYPGVTVSVRGLTYIV
                     REKQQFCSFVYDGGEVKVKPFDY"
     gene            complement(2866..>3550)
                     /locus_tag="P5219_02725"
     CDS             complement(2866..>3550)
                     /locus_tag="P5219_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HRX25701.1"
                     /translation="ENIVKRYLPLVKYVTARMAVSPPSGLDYDDLLSFGVFGLLDAID
                     RFDVSKGFSFQTFAVPRIRGAVLDELRKYDWISRTGREKLQRLGKAMETVLQDRGSLS
                     DQALMDVLGMDEKEYRETLELSSRSYIVSLDEVLSLEDGDVSRDGIIPDDGESAADLL
                     EDEDETVRVAGALKRLPEREMQVISLYYYEGLTLKEIGKVLGVTESRISQIHGKALAA
                     LRGILAAKE"
BASE COUNT          607 a         1128 c          982 g          833 t
ORIGIN      
        1 ttcgtgaagg ggaagagggc caccctttat tttcgtactc atcgccgccc cgtacagaac
       61 ggtccccagt gctctccggg gaggtctgag aggccggaca gccgccttcc gtttcaggta
      121 cctcgtctcc gggacactta ctccgggacg ccaggaccgg acggaaaccc tgccgagaaa
      181 aagcctggcg atattctccc ggagccggtc aagatccact cttttcctcg cccagggcgc
      241 tcatggcctt tcccattcgg gaaaagggct cgagaaggtc catggtggcc gctgcttcca
      301 cgaggatcga ggccgccgac cgggcgtcct cttccctgtt tttttcgccg ggcagggaga
      361 aacggagcgg tgcgctgaca ccgtcggcct gaacaatata ggatcccgga tcattctgct
      421 tttcaatatc gcccgtggca tccaggggag accgggccgt cggggccagc aggatcgtct
      481 ttgattttgc gagtttcgca agggggtcca gaagatcgcc ttccctgcag acccacagga
      541 ccggggtgac ctccacggct ccaacaagac tttccaggat cagctcttcc ttgatgtaga
      601 cgtcgagggc gttcccgtag agaattttct cgattttcga aggggtgatg ggatccgtgt
      661 accgaaagtc ggcgggaatg ccccggaaat ccgtcacaag gacggcgccc cgaaaggttc
      721 cctccgttcc gtcaacaagc acgtacccga tatttttgcc ggctgcctgg gatgccactc
      781 tctttcctcc ctcgtgcgtt tccgctcatg cgtaaaaatc aagggttccc ctgtccgccc
      841 cgggagaagg gggatccttg tccggttctt cgtcgtcgcg gggagaccgg gtttgtttgc
      901 ctcttttgcc gtccttttcc gactggtccc ttttcttctt cgcgccgagg cgctcttcct
      961 cggcggactg ctcagcggcc tgaacggtac tgtcacggtg aacggcagcg gcgactccct
     1021 ctccctggcg gccggcctga agcgacgcag ctgaaggatc cctcagctgc tgggcggtat
     1081 gctcgagatt ccagtgggcc agctgcatgt tcacagggct taccaccgtc gttcaccttc
     1141 ctttcgttca gtagtcgaag ggtttgacct tgacctctcc tccgtcgtag acgaaggagc
     1201 agaactgctg cttttcccgg acgatgtagg tgagcccccg aacggagacg gtcactccgg
     1261 ggtagcagat gcccttcacc cggacacatc cctttgcctt gctgttctcc atctgctcct
     1321 cgatgatcct gagttcctcc ctggcagtgc ccagctggct ctggagctgg aacttggcct
     1381 tcgtgaggct gatcataaga gcccgcttgt tctcgtcgag ctgtcccgcc ccttcaagct
     1441 tcttcaggaa caagaggttg gcctcgattt tttcaccctt ttcctcggac tcagcgagta
     1501 gggcaaggag ctgttttttt ctctccgtga gctcggcaag cacgccgacg acaacttctg
     1561 tttttgttcc catttcgctt cccagggtaa ggcagactac ttcgaggccg gcctgcacct
     1621 tccctcccgc gatctgggac ttctggccgc cgaggacagt gatgttgccg tgggcggcaa
     1681 ggtcgctgtg cagtacggca ttggtcacgg tgatgctctg accgctccgg acatgggcct
     1741 ggtcgatgaa gcccgcggtg atattgccgt ccgcctcgat cttcgccttg ttcatgcccc
     1801 gaatgccccc tgtgatggaa atgttccccg tgctggagag atgggccccc tccaccacgc
     1861 cccgtatctc gatgtcgccg gaggaaacaa tctcgaagcc ttccctgacg gccccgttca
     1921 ccaggacggc gccgatgaag ttgatgtttc cgacgctgta gtccacgtct ccgtccacct
     1981 ggaagacggg gaccacgtgg agcttgcccc ccttcaggac aagatttccg tcgatgaggg
     2041 acaggaggtg aattccgtcc tcggaggcgt atgttcccgc tcccgcagga agcggccggt
     2101 ccttcccctt ttttgccttg aggacggtgc ccttcacggt gatgccgtcc gttccttccg
     2161 tggccggaat tttctcggcg agcatctggt ttttcatcac cacggtcacc gaactcagat
     2221 tcttcaggtc cacccgtccg gattcgtcct cttccttcgg ctttgcggcc ccgaactgga
     2281 ccttgtagtc gaagtcggca tcggcaccgt cggttgcggg agttccggag gcgacggcag
     2341 tccaggatct gcctcttttt tcggaaagga tgtcggaaat cagccccttg tccactcctt
     2401 cgacgactcc ctgggcggac aggaactcca gaacctggcc caccgtgggc catggcttgt
     2461 ccgcgaaagg aggctcgatc cagagttccg cggacatggc atcggatgag atcttcactt
     2521 cgagggcggc ttccttctcc tgttccggat ccctctccgc cactttgacg ggagagccgt
     2581 ccttccggga gaggaaggtc atcaccacgt caccgttgta gttctccacg cctttcagtt
     2641 tcatgaagtt taccaccgac ggcatggagt agtccgcccc ttcggaagcc gaaaggtaga
     2701 cgccgtcctc cctgaattca agatggaacc tgtcgtctgc catttttttc ccccctggcg
     2761 cggggcatgg ccgcagcccg acgatccggc tgcgctgccc ctccgcggct gtttcttctc
     2821 ccggaaattc cccacagttc cgggatcgtg ccggtgaggc tctcctcact ccttcgcggc
     2881 gagtatgccc cgaagggccg cgagagcctt gccgtgaatc tgggagatcc tggattccgt
     2941 aactcccagg actttcccta tttccttgag tgtcagtcct tcataataat acagcgaaat
     3001 aacctgcatt tcccgttccg gcagcctttt cagggctccc gccacacgga ccgtttcgtc
     3061 ctcgtcttcg agcaggtcgg cggcgctttc cccgtcgtcg ggaatgattc cgtccctgct
     3121 gacatcgccg tcctccaggg agagcacttc atcgagggag acaatatagc tcctgctcga
     3181 cagttccagg gtttccctgt attctttctc gtccattccc aggacatcca taagtgcctg
     3241 gtccgagagg ctccccctgt cctgcagcac cgtttccatg gcctttccga gcctctggag
     3301 cttctctctg cccgtcctcg aaatccagtc gtatttcctg agttcgtcaa gaaccgctcc
     3361 ccggatcctg ggaacggcga aggtctggaa ggaaaatccc ttggacacgt cgaaccggtc
     3421 gatggcgtcc agaaggccga aaacgccgaa actgagaaga tcgtcataat ccagccccga
     3481 cggcggagaa acggccatcc tggctgtgac gtacttgacg aggggaagat accttttgac
     3541 tatattttcc
//
LOCUS       DAOTHD010000104         3549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_31214_length_3549_cov_3.179164, whole genome shotgun sequence.
ACCESSION   DAOTHD010000104 DAOTHD010000000
VERSION     DAOTHD010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31214_length_3549_cov_3.179164"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="P5219_02730"
     CDS             complement(<1..1238)
                     /locus_tag="P5219_02730"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HRX25702.1"
                     /translation="MQVPARDAVEEASAFLVNEVLSRGAAGADVVCSFGQGSSLSLRD
                     GVPEKNSSGTSFGIGLRTLDREGRQGVAHVNSLERRHLEELAAWSLNNCSSSEPDPHL
                     RLARQGRDVRPDLELFDEAVLRVTPEYRMAVCREMWEIARDADPRVVSVRSASWGEGS
                     GEYHYRSSEGVSGWYSGTSAGCGVSVILSGGDVMEMGGYGDDSRFLSGLDPRKVAAEA
                     VRRTALVLAGKSLPTGRYDLVLDGEAAASLVDVLGELFLASNIHKNKSFLRDKLGEKV
                     ASPALSLVDDGLLVRGMGSSPFDGEGVSCTTTRLLSDGVVTAWLYNLKYALLDGVAST
                     GNAGRSISGTPDVDCSNLLLLPGQWTPEDLLLQVGSGIYVTEFLGLHTINPVSGEFSL
                     GIKGASISGGALGGAVSGMT"
     gene            complement(1238..2266)
                     /gene="ltaE"
                     /locus_tag="P5219_02735"
     CDS             complement(1238..2266)
                     /gene="ltaE"
                     /locus_tag="P5219_02735"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HRX25703.1"
                     /translation="MFPIDLKSDTVTRPSKAMRQAMAEAEVGDDVYCEDPSVNRLQEK
                     ASAMTGKEAALFLPSGTMGNLVAVLTHCGRGDGAILGLNSHIYHYEGGGLAALGGVFP
                     LTADDSSGLIPSCEVERNCRPVNVHFAPAKLLCVENTHNKCGGLALSPAQMKETTDSA
                     RNRGLAVHIDGARIFNAAVAWKCDVKEYTALVDSIQFCLSKGLGAPIGSMLCGSAEFI
                     DRARHWRKKVGGGLRQAGIIAAAGLYALENNIGRLADDHGNAAMLASALSAGGLEVEK
                     NPMPTNMVYFHVPEKAGHDLHERCAARGVLFNGAAGGRIRLVTHIDVTREQVARAAEI
                     ILEEISAS"
     gene            complement(2403..3227)
                     /locus_tag="P5219_02740"
     CDS             complement(2403..3227)
                     /locus_tag="P5219_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRX25704.1"
                     /translation="MILVDLHLHSTFSDGSLTPEQLADQGKHRGLSVMSLTDHDTTAG
                     LPSFMAACSRLNVRAVAGIELSADYPSTMHILGYRLDFSHPSLEKTLESIRKARDWRN
                     SEMCRNLRKIGFDITLEEVEAEAAGDVVARPHMAKVMVRKGYVQDVASAFSRYLGNSG
                     PVYVNRERLSPEDCISLIREAGGIAVLAHPVQTADSYAEIRIIAGKLKEWGLWGLECI
                     SAKHSAEQIFQYLSIASELGLFPTAGSDYHGSGFLSAMGIPVPEDLLPWARLGVSL"
     gene            complement(3237..>3549)
                     /locus_tag="P5219_02745"
     CDS             complement(3237..>3549)
                     /locus_tag="P5219_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRX25705.1"
                     /translation="GFIVPVFDMELKIGMVQKELTDEARIVVVSWNEILFGIIVDSVR
                     EVCTIYDAQIESAAQINTSMDRKYILGVAKHEDGRLIVLLDLAVLFEIDSILDEEASG
                     S"
BASE COUNT          776 a         1050 c         1004 g          719 t
ORIGIN      
        1 atggtcattc ctgaaactgc acctcccagt gcgcctcccg aaatcgaagc gcccttgatt
       61 ccgagggaaa actccccgct cacggggttg atggtgtgaa gccccagaaa ctccgtgacg
      121 taaatgccgc tgcccacctg gagaagaagg tcttccggag tccactggcc cggaagaaga
      181 agaaggttgg agcagtccac gtcaggagtg ccggagatgg aacggcccgc atttcctgtg
      241 gatgccacgc cgtcgagaag ggcgtacttg agattataaa gccatgccgt gacgactcca
      301 tccgaaagaa ggcgggtcgt tgtgcaggac actccttccc cgtcgaaggg agatgagccc
      361 attccacgca cgagaaggcc gtcgtccacc agggaaagag caggggatgc cactttttca
      421 cccagcttgt ccctgaggaa ggatttattc ttgtgtatgt tggacgccag aaagagttct
      481 ccgagaacat ccaccaggga agccgcagct tcgccgtcaa ggacgaggtc gtagcgtccc
      541 gtggggagag attttcccgc gaggaccagg gcggttcgcc ggaccgcttc cgcggcaacc
      601 ttccggggat cgagccctga aagaaaccgg ctgtcgtccc cgtaccctcc catctccata
      661 acatctccgc cggagagaat caccgagacg ccgcagccgg cgctggttcc cgagtaccat
      721 ccggagactc cttcggagga acggtagtga tactcaccgc tcccctcacc ccatgacgcg
      781 ctcctgacgg acaccacccg aggatcggcg tccctggcga tttcccacat ttccctgcag
      841 acagccatgc ggtactccgg tgtgacacgc aaaaccgcct cgtcgaagag ctcgagatcg
      901 gggcggacat ccctgccctg ccgagcaagc ctgaggtggg gatccggttc ggaagaagag
      961 cagttgttca ggctccatgc cgccagttcc tcaaggtggc gcctctccag ggagttgacg
     1021 tgggcgaccc cctgccgtcc ctccctgtca agggttctca gcccgatgcc gaaagaagtg
     1081 cccgatgaat ttttctctgg aacaccatcc ctgagagaca gagagcttcc ctgcccaaaa
     1141 ctgcagacaa catccgctcc cgcagctccc ctggagagga cttcattgac cagaaatgcg
     1201 gaagcttctt caacggcgtc ccgggcagga acctgcatca cgaagctgaa atctcctcaa
     1261 ggatgatctc cgcagcccgg gcaacctgtt cccgggttac gtcgatatga gtgacgagcc
     1321 tgatgcggcc tccggcggca ccgttaaaaa ggacgcccct ggcggcacag cgttcatgga
     1381 gatcgtgccc tgccttttcc ggaacatgga aatacaccat gtttgtgggc atgggattct
     1441 tctccacttc cagccctccg gcggagagcg cggaagccag catggcagcg ttcccatggt
     1501 catccgcgag acgcccgata ttgttttcca gggcatagag cccggcggcg gcgatgattc
     1561 ccgcctgccg gagccctcct ccgacttttt tcctccagtg gcgggccctg tcgatgaact
     1621 ccgcgctccc gcagagcata ctgccgatgg gagcgccaag gcccttggag agacagaact
     1681 gtatggagtc aacaagggcg gtatattctt tgacgtcgca tttccaggcc acggcggcat
     1741 tgaagatgcg ggcgccgtcg atatggaccg cgaggcccct gttcctggct gagtcggtgg
     1801 tttccttcat ctgggcaggc gaaagggcga gtcctccgca tttgttgtgg gtattttcca
     1861 cgcagagcag tttcgccggg gcaaaatgaa cgttgacggg acggcagttc ctctcgactt
     1921 cgcaagaggg gatcagaccg gaagaatcat cggcggtgag agggaacacc cctccgaggg
     1981 cggcgagtcc gcctccctcg taatggtaga tgtggctgtt gaggccgaga atggctccat
     2041 cccctcgccc gcagtgggta agcactgcca cgaggttccc catggtaccg gaaggaagga
     2101 acagggccgc ttccttgccg gtcatggccg acgccttttc ctgcagcctg ttgaccgagg
     2161 gatcctcgca gtaaacatca tcccccactt cggcttcggc catggcctgg cgcatggctt
     2221 tcgacggacg ggttaccgta tcgctcttca gatctatggg aaacatggca aactcctcct
     2281 taaaatacga tgttggttcg ttccatcaga atatgataac atcaaaaaaa cgatttctgg
     2341 agggcaattc actccggaaa tcgccttttc tcctgcaaat tcaaaatctt ttcggcgaga
     2401 ttctacaatg aaaccccgag gcgcgcccat ggcagcaagt cctccggaac agggataccc
     2461 atggcggata aaaaaccgct tccgtgataa tcgcttcccg ctgtgggaaa tagcccgagt
     2521 tctgaagcga tggaaagata ctggaaaatc tgctcggcag aatgctttgc cgaaatgcat
     2581 tccagtcccc aaagccccca ctctttcagc ttcccggcta taatgcgaat ctccgcatag
     2641 ctgtccgcag tctggaccgg atgggctaga accgctattc cgccggcctc ccggatgaga
     2701 gaaatgcaat cctccgggga aaggcgctcc ctgttcacat atacggggcc tgagttccca
     2761 aggtatcgag aaaaggcgga tgctacatcc tgtacgtacc ccttccgcac cataaccttc
     2821 gccatgtgag gacgggccac cacatctccc gcagcttccg cttccacttc ctcgagggtg
     2881 atgtcgaaac cgattttccg gagatttcgg cacatttcac tgtttctcca gtcccgggct
     2941 ttccggatcg actcaagggt cttctcaaga ctggggtggg aaaaatccag ccggtaaccg
     3001 aggatgtgca tagtagaggg gtagtcggca gaaagctcta ttcccgcaac ggccctgacg
     3061 ttaagccgtg aacaggcggc catgaacgaa gggagccccg cggtagtgtc atgatccgtg
     3121 aggctcataa cggaaaggcc cctgtgtttc ccctggtcag cgagctgttc gggggtcagg
     3181 gatccatcgg aaaaggtgct gtgaagatgg agatcgacaa gaatcataca ggatcgtcag
     3241 cttccggagg cctcttcatc gagaattgag tcgatctcga acagaacggc gaggtcgagg
     3301 agaacgatga ggcgtccatc ttcgtgcttg gccacgccga gaatgtattt cctgtccatg
     3361 gacgtgttga tctgggcggc ggattcgatc tgggcgtcgt aaattgtgca gacctcgcgg
     3421 acggaatcca caatgatgcc gaaaagaatc tcgttccacg acactaccac gatacgggcc
     3481 tcgtcagtga gttctttctg caccatgccg attttcagtt ccatgtcgaa aacggggact
     3541 atgaacccc
//
LOCUS       DAOTHD010000105         3541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_31304_length_3541_cov_3.444636, whole genome shotgun sequence.
ACCESSION   DAOTHD010000105 DAOTHD010000000
VERSION     DAOTHD010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31304_length_3541_cov_3.444636"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /gene="upp"
                     /locus_tag="P5219_02750"
     CDS             <1..237
                     /gene="upp"
                     /locus_tag="P5219_02750"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRX25706.1"
                     /translation="PMLATGGSASAAISLVKARGGKKVSLVCIIAAPEGVSKVHGDHP
                     DVDIFAAALDSHLNDHGYIVPGLGDAGDRLFGTK"
     gene            247..1140
                     /locus_tag="P5219_02755"
     CDS             247..1140
                     /locus_tag="P5219_02755"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRX25707.1"
                     /translation="MEFALKNFFVAAPLLLLWGLCVTPLSISLANRYRIVDHPGERKI
                     HTSVTPRGAGIVLWLGFLLWCLFAAGEHPVVRFIGTGSTMVFLSGYRDDMKSLAPLVR
                     LGVHLFAAVFFLVPVRLPAAHLAVCLFWITGMTNAYNLIDGANGLCLLMFISACLLSS
                     PLGNTTVFLSLACLAGGVLYWNFPKARTFLGDGGTTLLGYLFSCFFIYTVAPGLGSVG
                     VIELPFLLLLAGGVPVADTLFAILRRVWKGRSPFLPDRGHIHHRLLDRGITPFWTVLI
                     LAFIQCLIVGSGIFLFTARLS"
     gene            1207..2331
                     /gene="wecB"
                     /locus_tag="P5219_02760"
     CDS             1207..2331
                     /gene="wecB"
                     /locus_tag="P5219_02760"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HRX25708.1"
                     /translation="MKKKIACVIGTRPEAIKMAPVISALRKKNLDVTVLATGQHSWML
                     DQALAFFEIRADVNLSVMEERQPLDLLTSRVLLGVGSFLDNSPQDLLLVHGDTTTTMA
                     AAMAAFYRHIPVGHVEAGLRSGDMGRPFPEEANRIITDRLSTLWFAPTEGAAENLRRE
                     KLPVSSKTLFVTGNTVIDALFGTLRKNHTPSPELAPLAQENFPVILMTAHRRESWGSP
                     LESICRAIKDILEADDRVRVLIPLHKNPSVRDVIQRILAGERRIILCEPLNYPDFVWA
                     MNRSILILSDSGGVQEEAAGLKKPVLVMRDLSERPEALETGTALLVGTDRERIKETTL
                     KILSDGAFREKLLNRGINPFGDGSASLRIADSVLGYYEGK"
     gene            2390..>3541
                     /gene="murA"
                     /locus_tag="P5219_02765"
     CDS             2390..>3541
                     /gene="murA"
                     /locus_tag="P5219_02765"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRX25709.1"
                     /translation="MESKMEIIGGTALRGVVEAQGAKNASLPVMAAALLLKDSTLHIS
                     RVPDLLDVNTMADLLRSLGAEVAFSSHNMKIYTPAEISWETPPDLVRKMRASSLVLGP
                     LLARCGKAVMPLPGGCSIGSRPIDLHLKGLTKMGATIELVHGAVHAHTTGLTGCRIYL
                     DFPSVGATENLMMAAVFARGGTVLENTAREPEIYNLVDALKSMGAQVEADGTGVIRIQ
                     GVDVLGSAESRIIPDRIEACTYILAGAATNGEVTVTGVIPNHIDSLLAKLEEAGASLT
                     VNESEVTIHPSSRLKGVTLKTLPYPGFPTDLQPQIMAALCLAQGTSVVQESVFQSRFL
                     HVSELNKMGAKIDIQSNSAIVTGVDRLTGADVVATDLRAGAALVIAGLAA"
BASE COUNT          754 a         1016 c          977 g          794 t
ORIGIN      
        1 cccatgctgg ccaccggcgg ctcggcttcg gccgccataa gcctggtgaa ggctcggggc
       61 ggaaagaaag tctcccttgt gtgcatcatc gctgcccctg aaggcgtatc gaaagttcac
      121 ggagatcatc cggatgtgga catcttcgcg gcagccctgg acagccatct gaacgaccac
      181 ggttacatcg tgcccggcct cggcgacgcg ggagacaggc ttttcggcac aaaataggcc
      241 attcccgtgg aattcgcgct gaagaatttc tttgttgcgg ccccgctgct tctgctttgg
      301 gggctctgtg tcacgcccct ttccataagc ctggcgaacc gctaccgcat cgttgatcat
      361 cccggggagc gaaagattca tacgtcggtt accccccggg gagcgggaat tgttctctgg
      421 cttggttttc ttctctggtg ccttttcgcc gccggagaac acccggttgt caggtttatc
      481 ggtacgggga gcaccatggt tttcctcagc ggatacaggg acgatatgaa gagccttgct
      541 ccccttgtgc ggcttggtgt gcaccttttt gccgccgttt tctttcttgt gccggtacgg
      601 ctgcctgcgg cgcatcttgc tgtctgtctt ttctggataa cgggaatgac caatgcctac
      661 aatcttatcg acggcgccaa cgggctctgc cttctcatgt tcatatcggc gtgccttctc
      721 tcctcacccc tcgggaacac cacggttttc ctctctctcg cctgccttgc gggaggagta
      781 ctgtactgga attttccgaa ggccagaacc tttctcggag atggcgggac aacgcttctc
      841 ggctaccttt tttcgtgctt tttcatttat actgtggcac cgggacttgg atccgtaggg
      901 gtgatcgaac ttcccttcct gctgcttctt gccggcggcg ttcccgtagc ggataccctt
      961 tttgccatcc tgcgcagggt gtggaaagga aggtctccct tcctgcctga cagggggcac
     1021 atacatcacc gtcttctcga caggggaatc acaccattct ggacggttct tattcttgct
     1081 ttcatacagt gccttatcgt tggttcaggg attttccttt tcactgccag gctctcctaa
     1141 atgttctttt tccctgaaca ggaaaagatt gcagcggaca ccatgaaaga cggagttgtt
     1201 tcattcatga agaaaaaaat agcatgcgta ataggaacac gcccggaggc catcaaaatg
     1261 gcgccggtga tttccgcctt gaggaaaaaa aatctggacg tcactgtcct ggccacgggc
     1321 cagcattcct ggatgctcga ccaggccctt gcattttttg aaatcagggc tgacgtgaac
     1381 ctttccgtca tggaggaacg acagcctctg gatcttctca catcacgggt acttctcggc
     1441 gtgggcagct ttctggacaa ttctccccag gatctcctgc ttgttcacgg agacacgacg
     1501 accaccatgg ctgccgcgat ggccgcgttt tacaggcata tccccgtagg ccacgttgaa
     1561 gcggggcttc ggagcggcga catggggcgc cctttccccg aggaggcaaa caggatcata
     1621 acggacaggc tctctacgct ctggttcgcc ccgacggagg gagcggcgga gaacctgcgc
     1681 agggaaaaac ttccggtttc ttccaaaacc ctgttcgtaa caggcaatac cgtcatcgat
     1741 gccctgttcg gcaccctcag gaaaaaccac acgccgtcac cggaacttgc accgctggcc
     1801 caggaaaact ttccggtcat cctgatgacc gcccacagga gggaatcatg gggaagtccc
     1861 ctcgaatcca tctgccgtgc cataaaggac attctggagg ctgacgaccg cgtccgagtc
     1921 cttattcccc ttcacaagaa cccgtcggta cgggatgtga ttcaacgcat tctcgcaggc
     1981 gaacggagaa tcatcctctg tgaacctctc aattatccgg attttgtctg ggcgatgaac
     2041 aggagcattc ttatcctcag cgacagcgga ggagttcagg aagaagcggc gggattgaag
     2101 aagccggtgc ttgtcatgcg ggatctttcc gaacgcccgg aggctctcga gaccgggacg
     2161 gctctccttg tgggaacgga ccgcgaaagg ataaaagaaa ccacattgaa aatcctttcc
     2221 gacggagctt ttcgggagaa actgctgaac aggggaataa accctttcgg tgacggatcg
     2281 gcatcgctga ggatagccga cagcgttctc ggttattacg aaggcaaata aaatcgtggt
     2341 atagtagctt aatatgattt cccaccaaaa tttcgggagg aattgaccag tggaatccaa
     2401 gatggaaata atcggcggta cagccctcag gggagttgtc gaagcccagg gagcgaagaa
     2461 cgcgtccctt ccggtaatgg ccgccgctct tcttctaaag gacagtactc tccatatttc
     2521 ccgcgtgcct gatcttctcg acgtgaacac catggcggac ctgctcagaa gcctcggagc
     2581 ggaagtcgca ttcagcagcc acaacatgaa gatctacacc cctgcagaaa tatcctggga
     2641 aacccccccg gatctcgtga gaaaaatgcg agcctcgtcc cttgttctgg ggcctcttct
     2701 cgcccgatgc ggcaaggccg tcatgcctct gccgggggga tgctccatcg ggagcaggcc
     2761 cattgacctt cacctgaaag ggctcacgaa gatgggggcg accatcgaac tggtgcacgg
     2821 agcagtacac gctcacacca ccgggttgac cggatgccgc atttacctgg attttccctc
     2881 ggtaggagcc acggagaatc ttatgatggc cgcagttttt gctcgagggg ggactgtgct
     2941 tgaaaataca gcccgggagc cggaaatcta caaccttgtg gatgccctga aatcaatggg
     3001 agcccaggtg gaagcggacg gcacgggtgt catccgcatc cagggagtgg acgtactcgg
     3061 aagcgccgaa tcaaggatca ttccagaccg catcgaggcc tgcacttaca tactcgccgg
     3121 agccgccacc aacggtgagg tgacggtgac cggcgtcata cccaaccaca ttgattctct
     3181 cctcgcgaag ctggaggaag cgggcgcctc tctcacggtg aacgaaagcg aggttaccat
     3241 ccacccttct tccaggctga agggagtaac cctaaaaact ctcccatatc cgggattccc
     3301 caccgatctt cagccccaga tcatggctgc cctgtgcctg gcccagggga ccagcgtggt
     3361 ccaggagagt gtttttcagt cccggttcct tcatgtcagt gaactgaaca aaatgggagc
     3421 gaaaattgac atccagagca actcggccat cgtcacaggc gtcgacaggc ttacaggagc
     3481 cgatgtggta gccaccgacc tgcgggcggg agcagccctt gtcattgcgg gactggctgc
     3541 c
//
LOCUS       DAOTHD010000106         3530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_31421_length_3530_cov_3.018417, whole genome shotgun sequence.
ACCESSION   DAOTHD010000106 DAOTHD010000000
VERSION     DAOTHD010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31421_length_3530_cov_3.018417"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="P5219_02770"
     CDS             <1..595
                     /locus_tag="P5219_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HRX25710.1"
                     /translation="HTLVMVAINSLTMLFLYGPLGGFLLGVGRIPVPWQALALSIAVY
                     VALPLAAGYISRKMILRTKGEAWFREKFLHFLTPVTIAALLITLVLLFSFKGEVIVAN
                     PLTILWIAIPLFLQTVLIFGLGYWLSKKLGLSYQDAAPSAMIGASNHFEVAIATATML
                     FGLSSGAALATVVGVLIEVPVMLMLVRFCLKTEKWFA"
     gene            778..1143
                     /locus_tag="P5219_02775"
     CDS             778..1143
                     /locus_tag="P5219_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRX25711.1"
                     /translation="MKTKEKLQEEKAAILKGIAHPVRLRVVEALACREMCVCEIAELF
                     DFDRTTISKHLSLMKGLGILDSRKDGLNVMYSLRMKCLASLLSCVEKVVLGQEADMSA
                     LFCGCGSLSNNDREEELNA"
     gene            1140..1382
                     /locus_tag="P5219_02780"
     CDS             1140..1382
                     /locus_tag="P5219_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRX25712.1"
                     /translation="MKIQILGTGCPKCKKLADLAEQAARELGLAYELEKVTEIPKIIA
                     FGVVTTPGLAVDGKVLAAGSLPSYEKVKDLLAASAQ"
     gene            1477..2643
                     /locus_tag="P5219_02785"
     CDS             1477..2643
                     /locus_tag="P5219_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRX25713.1"
                     /translation="MSDRKKFFWIALFFLTFYFLPEGNERVIAGLTESVAMMADYARE
                     HVLLCLIPAFFIAGAISVFVSQQSVMKYLGAKAKKGIAYSVASVSGTVLAVCSCTVLP
                     LFAGIYMRGAGLGPATAFLYSGPAINVLAIVLTARVLGLELGIARAVGAVIFSVVIGL
                     LMAAIFGREEQTRQEGFAQLPEEEGGRPLWKTVVYMASMVVFLVFANWAAPKTSSAFW
                     EGVYNLKWVLSGAGLAVTVLTAWMWFTRDERQDWYASTWGYALQVMPLLFGGVLVAGF
                     LLGRPGHEALIPSRYVAMLVGGNSLGANFFASISGAFMYFATLTEVPILQGLIGAGMG
                     KGPALALLLAGPALSLPSMLVINSILGPKKTVTYVVLVVLLSTVAGMLFGSFVS"
     gene            complement(2763..2838)
                     /locus_tag="P5219_02790"
     tRNA            complement(2763..2838)
                     /locus_tag="P5219_02790"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2803..2805),aa:Val,seq:cac)
     gene            3030..3482
                     /locus_tag="P5219_02795"
     CDS             3030..3482
                     /locus_tag="P5219_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25714.1"
                     /translation="MTEISDLGLVSDLWEYWGFSPWNSEGMKGVYRRVTFVKSALIGE
                     VCRYYADDYIIWSHNGKADRQRILKSCRPKPDLMTQRYLFVEGAESGEKCAIRSFLFG
                     FRGYAEVHSFTPGGRFEKRIKDLAPLVDKALELLRSRKNESGGGAPEK"
BASE COUNT          716 a          930 c         1049 g          835 t
ORIGIN      
        1 gcacactctt gtgatggtgg ccatcaactc cctcaccatg ctctttctct atggccccct
       61 tggcgggttc ctcctcggtg tggggcgaat tccggtgccg tggcaggccc tcgcactctc
      121 cattgccgtc tacgtggcgc tgcccctggc ggcgggctac atttccagga aaatgatcct
      181 caggacaaaa ggggaggcct ggttccggga gaagttcctt cacttcctga cgccggtcac
      241 catcgcggcc ctgctcatca cgctggtgct gctcttttcc ttcaagggag aggtcattgt
      301 cgccaacccg ctgacgatcc tctggatcgc tattccgctc tttctccaga ccgtactgat
      361 cttcggcctg ggctactggc tctcgaaaaa gctcggcctg tcctaccagg atgccgcgcc
      421 gtccgccatg atcggcgcgt ccaaccactt cgaggtggcc atcgcaacgg ccaccatgct
      481 gttcggcctc tcctcggggg ctgccctcgc cactgtggtg ggggtgctca tcgaggtgcc
      541 ggtgatgttg atgctggtca ggttctgcct gaagacggag aaatggttcg cctgaggaaa
      601 aaacggcact gttgcttgac agattggctt accgcagccg aaaaccatca tcaaaacgga
      661 aaaaggcggg agaaatatcc gtgacgggga aaatatgtgc ctgaaagcgg ctcgcttcaa
      721 gggggttgac gttttttcaa agcgtggtaa tattgccaca tagcgggggg tgagcgattg
      781 aaaacaaaag aaaaactgca ggaggaaaaa gcggcgattc tgaaagggat tgcccatccg
      841 gtccgccttc gggtagtgga agcccttgcc tgccgggaga tgtgcgtctg cgaaattgcc
      901 gagctcttcg attttgacag gaccaccatc agcaagcatc tttcgctgat gaaaggcctg
      961 ggcatcctgg acagccggaa ggacggactg aacgtgatgt acagcctgag gatgaaatgt
     1021 ctcgcctccc ttctttcctg cgtggaaaaa gtggttcttg ggcaggaggc ggacatgtcc
     1081 gcactgttct gcgggtgcgg ttctttatca aacaatgacc gtgaggagga attaaacgca
     1141 tgaaaataca gatcctgggg acggggtgcc ctaagtgcaa aaaactggcc gatctggccg
     1201 aacaggccgc cagagagctc gggctcgcct acgagcttga aaaggtcacg gagattccga
     1261 aaatcatcgc tttcggagtg gtgacgacac cggggcttgc ggtcgacggc aaggttctcg
     1321 cagccgggag cctgccctct tatgaaaagg tgaaggatct tcttgcggct tcggcgcaat
     1381 agcgtagaaa gacgagtccc cgggacgacc gggggttttt tttgaacagt ttgagtgaag
     1441 aaattgtcac atatttgcat tggagatgat tcgttcatga gcgaccgcaa aaagtttttc
     1501 tggattgcat tgttcttcct gaccttttac ttcctccccg agggaaatga acgggtgatc
     1561 gccggtctta cagaatcggt ggccatgatg gccgactatg ccagggaaca cgttcttctc
     1621 tgcctgatcc ccgcattttt catcgcgggt gccatttcgg tgttcgtgag ccagcagtcg
     1681 gtgatgaagt atctgggggc aaaggcgaag aaagggattg cctactccgt ggcctccgtc
     1741 tccgggacgg tgctcgcagt gtgttcctgc accgtgctgc ccctcttcgc cgggatttac
     1801 atgcggggcg ccgggcttgg cccggcgaca gcatttttat actccggacc tgcaataaat
     1861 gtgctggcca tcgttctcac ggcccgggtg ctcggccttg agctgggcat cgccagggcg
     1921 gtgggggcgg taatattcag cgtggtgatc ggtcttctca tggcggccat tttcggcagg
     1981 gaggaacaga cccgacagga ggggttcgcc cagctccctg aggaagaggg cggccgccct
     2041 ctatggaaga ccgttgtgta catggcgagc atggtggttt ttctcgtctt cgccaactgg
     2101 gcggcgccga agacctccag cgccttctgg gaaggggttt acaacctgaa gtgggtgctc
     2161 tcaggggcag ggcttgccgt cacggtgctg accgcatgga tgtggttcac ccgtgacgag
     2221 cggcaggact ggtatgcgtc cacgtgggga tacgctctcc aggtcatgcc cctgctcttc
     2281 ggcggtgttt tggtggcggg cttcctgctg ggccgtcccg gccacgaggc ccttatcccc
     2341 agccgttatg tggccatgct cgtgggaggg aactccctcg gggccaattt cttcgcctcc
     2401 atttccggcg cgttcatgta tttcgccacc ctcaccgaag tccccatcct ccagggactg
     2461 atcggcgccg gcatgggcaa gggccctgcc ctcgccctgc tccttgccgg cccggccctc
     2521 tcgctgccca gcatgctggt gatcaacagc atcctggggc ccaaaaaaac ggtgacctac
     2581 gttgtgcttg tcgttcttct gtccaccgtg gcgggcatgc tgttcggcag ctttgtttcc
     2641 tgacctgaaa ggtgtggatt aaagaagaaa ggacatcttt tttttgggtg gaatgaggat
     2701 ttcatgactc ttccctgtaa aagaagaggc tccggcaaaa tgccggagcc tctgttcatt
     2761 catggtgggc gatactggga tcgaaccagt gacctcttcc gtgtgaagga agcgctactt
     2821 ccgctgagct aatcgcccga aaacaggagg tattataccg tgcccggctg tgctttgcaa
     2881 gccggaaaat ctttggaaga caccgtcttg gttcatcatt ctccagaaaa ccatactgaa
     2941 tttttgatag aaaatatggc ggaaaggaac ttaaatcact tcctattttt tctatgagga
     3001 gtataatctg ttcggcaggt gatgagtgag tgaccgaaat ttccgatttg ggacttgttt
     3061 ccgacctgtg ggaatactgg gggttctctc cctggaattc cgagggaatg aaaggagtct
     3121 atagaagggt tacattcgtg aagagcgcgc tgataggtga ggtatgcaga tattacgccg
     3181 atgattacat aatctggagc cacaacggca aagctgacag gcagcggatt ctgaagtcct
     3241 gtaggcccaa gcccgacctc atgacgcagc gctatctttt tgttgaggga gcggaatcgg
     3301 gggagaaatg cgccatccgg tctttcctct tcgggttccg cgggtatgcg gaggtacact
     3361 cctttacgcc cgggggcaga tttgagaaac ggatcaagga tcttgcccct ctcgtggata
     3421 aagcactgga gcttctccgc tcacggaaaa acgagtctgg aggtggtgcc ccggaaaaat
     3481 agaggctgca tcagtttttc acactctttt catattttcc ggaggtgaat
//
LOCUS       DAOTHD010000107         3455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_32311_length_3455_cov_3.236176, whole genome shotgun sequence.
ACCESSION   DAOTHD010000107 DAOTHD010000000
VERSION     DAOTHD010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32311_length_3455_cov_3.236176"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="P5219_02800"
     CDS             <1..196
                     /locus_tag="P5219_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRX25715.1"
                     /translation="FTPLSAYAFLVMTLLYVPCVAAVAAFRRETGSWKWTFFMIAYTT
                     LVGYGGAVLVYQGGRLLGLG"
     gene            326..1201
                     /locus_tag="P5219_02805"
     CDS             326..1201
                     /locus_tag="P5219_02805"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HRX25716.1"
                     /translation="MDLLFEPLSLEKADRYRELYEACPRRSSYYSFGSLWAWRNIFGF
                     SWAFREGMCWIRTDSGSLWAPVGPWEAADWRRILPGLFPGRAEFSYAPDGLARIWTEL
                     FRDRIVAREVRSQWEYLHSVQDLIALRGNRFSRKRSHIRQFVKHYAFRYRSLGSDDGE
                     TILAAQRLWLSERPESPALLRENGAVEEMVREWRNIPGLLGGLLEVDGMPAAYTIAEA
                     IPGDTVMIHFEKALSSYNGAYQAINRLFLQNTAPSFTTVNREEDLGDEGMRVAKMSYH
                     PVGFLKKYTLSWFPD"
     gene            1186..1809
                     /locus_tag="P5219_02810"
     CDS             1186..1809
                     /locus_tag="P5219_02810"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRX25717.1"
                     /translation="MVPRLTHSSLSWRSGGFTLAEVLVVLALFGLIAALAFAPSVVLV
                     RGLEDTRAEMAKEQVSDFLLGRIAAEMRLSPRSFPGGPAVAAVRKEVLGGLADDRIAF
                     WSDYGGEPGVRAWKVFRPGAGRDGAPGVYRWILPLASPGAVDWENLDPGEGKLILPGA
                     DFLRISILSAETGEWGDDYEGPRPRGVRFEARAGKEAFSREDWLPPE"
     gene            1784..2719
                     /locus_tag="P5219_02815"
     CDS             1784..2719
                     /locus_tag="P5219_02815"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HRX25718.1"
                     /translation="MRTGFLRNDGDPKKRKGLVLLSVLLVSLFLLTASTGFALFARRS
                     VRAFDGRQRAFTARMVCEASLPAAKALLELHPGKGHAPGDDVFSPRILGFPEAGVTVE
                     MTITPLNGRFPLNGIFLPDGRTVRSELAGPWRRLWRRAGAENIEAAVLDFLDGDGEPR
                     LGGGERDGYLNRPLLSLNELLFVPGMTADILYGTETRPGIAPLVTLRSDGKINANTAL
                     PEVLALLDGLDGNIAADLVRARETRVFTSMEDLSAVPSFPASARAQLMNVLSFTSSHF
                     RVSFTVGFADGQKTPLEVILERKGSAAETVRWEEP"
     gene            2912..>3455
                     /locus_tag="P5219_02820"
     CDS             2912..>3455
                     /locus_tag="P5219_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25719.1"
                     /translation="MRSALSLRFLPLLSGEETVEVIPWISGSGSGLSEGAAWCVAAPE
                     VPAEESVSLSGIACWPLPLALASPVDGEGIAVFRGMGVVASAVFSRGIPLFCRCGSGE
                     EETPASEEGDGMDSEVRLCREFALASGHGELLSSVWTGTSPGELLEAARQTAERFPAF
                     LSVDISRPALEASLARERTAR"
BASE COUNT          560 a         1120 c         1098 g          677 t
ORIGIN      
        1 cttcaccccc ctctcggcct atgccttcct cgtgatgacc ctgctctatg tcccctgcgt
       61 agcggcggtg gcggccttca gacgagagac gggaagctgg aaatggacct tcttcatgat
      121 cgcctacacc accctcgtgg gctacggcgg cgcggttctc gtctaccagg gaggccgtct
      181 cctcggcctc gggtaaggaa aagcaccttt ccggccccct tccccgcccg gggaaggggg
      241 ctttttctct ttttcctccc gggtctggta gaatgagcgg aaaacaccag cagtaccggc
      301 gaacaaggaa aggacgatgg tgtccatgga tcttctcttc gaacccctct ccctggaaaa
      361 agcggaccgc tacagagagc tctacgaagc ctgccccagg cgttcatcct actattcctt
      421 cggcagcctc tgggcctggc ggaacatctt cggcttctcc tgggcctttc gggagggcat
      481 gtgctggata cggaccgatt cgggctccct gtgggccccc gtgggcccct gggaagcggc
      541 ggactggcgg aggatcctcc ccgggctctt tcccgggagg gctgaattct cctatgcgcc
      601 ggacggtctg gcaaggatat ggacggaact cttccgggac aggattgtcg cccgggaagt
      661 gcgctcccag tgggagtacc ttcattccgt ccaggatctc atcgccctga ggggcaaccg
      721 cttttccagg aagcggagcc acatccgcca gttcgtgaaa cattacgcct tccggtaccg
      781 ctccctgggt tccgacgacg gggagaccat cctcgcagcc cagaggctgt ggctctcgga
      841 gcgccccgaa tccccggccc ttctccggga gaacggggcg gtggaggaaa tggtccggga
      901 atggcggaac atccccggtc ttctcggggg gcttctcgaa gtggacggaa tgccggcggc
      961 ctacaccatc gccgaggcca tccccggaga cacggtgatg atccatttcg aaaaggccct
     1021 gtcatcctat aacggcgcct accaggccat caaccgcctg tttctccaga acaccgcccc
     1081 ctccttcacc acggtgaacc gggaggaaga cctgggagac gaggggatgc gggtggcgaa
     1141 gatgtcctac catcccgtgg gctttctgaa aaagtacacc ctctcatggt tccccgactg
     1201 acccactcct cactctcctg gcgttccggg ggatttaccc tggccgaagt cctggtggtc
     1261 cttgcccttt tcgggcttat cgccgccctg gccttcgccc cgtcggtggt cctcgtcagg
     1321 ggactcgaag acacgagagc ggagatggcg aaggaacagg tttcagattt tctcctgggg
     1381 cggatcgccg cggagatgcg tctctccccg cgctccttcc cgggcggccc ggcggtggcg
     1441 gccgtcagga aggaggtcct cggaggcctg gccgacgacc ggatcgcctt ctggagcgac
     1501 tacggcggcg agccgggagt acgggcatgg aaggtgttcc gcccgggagc cggcagggac
     1561 ggagctcccg gggtataccg ctggatcctt cccctggcgt cccccggggc ggtggactgg
     1621 gaaaaccttg atcccggcga agggaagctc atccttccgg gcgcggactt cctcaggatc
     1681 tccattctct ccgccgaaac gggggaatgg ggcgacgact atgaaggccc gaggccgagg
     1741 ggcgtccgct tcgaggcccg ggcagggaag gaggcgtttt cccgtgagga ctggcttcct
     1801 ccggaatgac ggggacccga aaaaacggaa gggcctcgtc ctcctctcgg tcctcctggt
     1861 gtcccttttc ctcctgacgg cctccacggg attcgccctc ttcgccagga ggtctgtgcg
     1921 ggcctttgac gggcggcagc gggccttcac ggcgagaatg gtctgcgagg cctccctccc
     1981 ggccgcgaag gccctgcttg aactccaccc cggaaaaggc catgccccgg gggacgacgt
     2041 cttttctccc cggatcctcg gatttcccga agcgggcgta accgtggaaa tgaccatcac
     2101 ccccctgaac ggccgtttcc ccctgaacgg catttttctc cccgacggac ggacggtgag
     2161 gtccgagctc gccggcccgt ggagacggct gtggcggagg gcgggagcgg aaaacatcga
     2221 ggcagcggtg ctggactttc tcgacggtga cggtgagccc cgcctcggcg ggggcgagcg
     2281 ggacggatat ttgaacaggc cccttctctc tctgaacgag ctgctgtttg ttccgggaat
     2341 gacggcggat atcctgtacg gaacggaaac acgccccgga atagcccccc ttgtcaccct
     2401 ccggtccgac gggaagatca acgccaatac ggcgctcccg gaggtgctgg ccctcctgga
     2461 cggcctggac gggaacatcg ccgccgacct tgtccgggcg agggagacca gggtcttcac
     2521 gtccatggag gacctctccg ccgtgccgtc ctttccggcg tcggcccggg cacagctcat
     2581 gaacgtgctc tcctttacga gcagccactt ccgggtgtcc ttcaccgtcg ggtttgccga
     2641 tggtcaaaag acccccctgg aggttatact ggaacgaaaa ggttcagcgg ccgagacggt
     2701 ccgatgggag gaaccgtaaa ttatcagacg gcagatttcc tcattttttc tcaagacacg
     2761 ggacggcttc cgcctcctcg aaagggtcgg cagtcctttt gcggaaaggg gctccgggaa
     2821 caggtttcct tcccggggcg atcttgttct gtatcccttc cgcactctct cggtacaccc
     2881 tttttccttt cccttccgtt cactgaagga cgtgcgcagc gccctctccc tcaggtttct
     2941 ccccctgctc tccggggagg aaaccgttga ggtcattccg tggatctcag gctccgggag
     3001 cggactttcg gaaggagcgg cgtggtgcgt ggctgcaccg gaggttcccg ccgaagagag
     3061 cgtttccctt tcggggattg catgctggcc ccttcccctg gccctggcct cccctgtgga
     3121 cggggaaggg atcgccgtct tccgcgggat gggggtcgtt gcctcggctg ttttctcccg
     3181 gggaatcccc ctcttctgca ggtgcggcag cggggaagag gaaaccccgg cttctgaaga
     3241 gggagacggc atggacagtg aggtccgtct ctgcagggaa ttcgccctcg cttccggtca
     3301 cggtgagctc ctttcgtcgg tctggacggg gacatccccc ggcgagctcc tggaagcggc
     3361 ccggcagacc gcagaacgtt ttccggcctt tctgtccgtg gatatctccc ggcccgccct
     3421 cgaggcctct ctcgcgaggg aaaggacggc caggg
//
LOCUS       DAOTHD010000108         3445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_32436_length_3445_cov_3.817994, whole genome shotgun sequence.
ACCESSION   DAOTHD010000108 DAOTHD010000000
VERSION     DAOTHD010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32436_length_3445_cov_3.817994"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="P5219_02825"
     CDS             complement(<1..757)
                     /locus_tag="P5219_02825"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRX25720.1"
                     /translation="MEKLFGYAGTVLWIDLTSGGISRKDLSAETIRPWLGGTGYAARL
                     LFDGVPPGADPLGPENLFILATGPLTDNVVPGGGSLSVCCKSPLTGGWGEARVGCDFG
                     PELKRAGYDFVVLSGVSDRPLHIVITDGTVELRDGAFLAGRKVLEREKLVRELDGLED
                     HSILTIGPAGEKGVLFASVMVGHRAAGRIGAGAVLGSKNVAALSVKGTGRIAPADPAA
                     WMREVRAAHGIVKENPNTPGFTELGTMGGYENSD"
     gene            937..1467
                     /locus_tag="P5219_02830"
     CDS             937..1467
                     /locus_tag="P5219_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25721.1"
                     /translation="MGVGHHLRGNAAQEEPFHCPQPPVADHDHERVIFNGEFHDLVVH
                     LPRPDRDGHFGNGLPENIHFLPDKLSELGLHFIVGGGNHYRSFSVEHRLMLGKSDDME
                     KEHVGIRLAIGIIDGVVHHSPAGLAPIDRHDHFLHLNPSFAALEKKADFLPVSPAMEY
                     SFMAIIAQNTLSRLNF"
     gene            1578..2462
                     /locus_tag="P5219_02835"
     CDS             1578..2462
                     /locus_tag="P5219_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRX25722.1"
                     /translation="MVNRSGFFRAALLFLLFFLATGSWSPVLAQDDATTHIELILDAS
                     GSMWAKLGGSTRIEVAKDALGKIIDDLSERKGIAVGLRVYGHRTNDCKDTKLEIPIGP
                     MDEKKMKAFIGKIKPKGKTPIAYSLQEAAKDFKKDFTGSKVIILVTDGLESCGGDPCA
                     AAKVLAEKGIVSKIHVVGFGMDKKSVSKLECIVKPSGGLLLEANSAAELAKAFDTIVK
                     TALDTNLEVTGLDGKGKPVEMSVSVLQEGDEVLSEKGQTVKANLPREVTSCGRQRRRQ
                     AKRSSSRVWTLWRTSSPP"
     gene            2381..>3445
                     /locus_tag="P5219_02840"
     CDS             2381..>3445
                     /locus_tag="P5219_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25723.1"
                     /translation="MRATATETGEEIFFEGVDLVEDKLTSLKAVFSIARIRVRALDSA
                     GKPVRAEWQIFRKDAPEEPVVRFSGADWTEKALSPGEYTLKAHHKDTKVTLTADAAPG
                     NGETAAVELVFAQGKLLISGLDSNGKPVYTDSYVYKAPFDRDNPDEAARDGGNKHEYS
                     LVPGTYDILVRDHNTKVEQWIRDVVIEGGKTVRKEVSFAQGKLIISGLDSNGKPLYTD
                     SYVYKAPFDRDNPDEAGRDGGNSHEYSLVPGTYDILVRDGNTKVEQWIRDVVIEGGKT
                     VRKEVSFSQGTVRLTVKGFEGKPVYFFTEIFLSPAEENDEFVHSDSGENEVSFPIVPG
                     TYDVKVWTEEPRAELWEKGME"
BASE COUNT          744 a         1035 c          997 g          669 t
ORIGIN      
        1 ggtcggagtt ctcgtaccct cccatggtgc cgagttccgt aaacccaggc gtgttgggat
       61 tttccttcac gattccatgg gcggcgcgca cctcccgcat ccaggctgcg gggtcggcgg
      121 gggcaattct tcccgtcccc ttcacagaaa gggctgccac gtttttcgaa ccgaggacgg
      181 ctcccgcgcc aatgcgcccg gcggccctgt gcccaaccat gacggaagcg aagagcacgc
      241 ctttttctcc cgcaggaccg atggtgagga tggaatggtc ttcaaggcca tccagttccc
      301 tcacgagctt ctctctttcc agcaccttcc ttccggcgag aaaggctccg tcccggagct
      361 ccacggtgcc gtcggtgatg acgatgtgca gggggcggtc cgatacgccg gagagcacga
      421 cgaagtcgta ccccgcccgc ttcagctcag gtccgaagtc gcacccgacc ctggcctcgc
      481 cccatcctcc ggtgaggggg gacttgcagc agaccgacag gcttccgccg cccggaacga
      541 cattgtccgt gagaggaccg gtggcgagta taaacaggtt ttccgggccc agggggtccg
      601 ctccgggagg caccccgtcg aaaaggaggc gggcggcata ccccgttcct ccaagccagg
      661 gccgtatggt ttcagcggaa aggtccttcc gggaaatccc gccggacgtg aggtcgatcc
      721 acaaaacggt gccggcgtag ccgaacagtt tttccatgag accagctcct tttctccttt
      781 tactatacca gacgccgccg cggggattcc ctgcgtgaaa tacgccccgc ccggaccccg
      841 ggcggggcgc tcttcccctt catttctgag ctttcggatt aaaaggtgct catttcgcct
      901 ctgcttcggg cctcggccta tggacataga cgctgcatgg gcgttggcca ccaccttcgc
      961 ggcaacgctg cccaggaaga accgttccat tgcccccagc ccccggtggc cgatcatgat
     1021 catgaacgcg tcattttcaa cggcgaattt cacgatctgg tcgtacacct tccccgacct
     1081 gacagagatg gacattttgg gaatggtctt cccgagaaca tccatttcct tcctgataaa
     1141 ctctccgaac tcggccttca cttcatcgtc ggtgggggga accactaccg ttccttctcc
     1201 gtagaacaca ggcttatgct cggaaagagc gatgacatgg agaaagagca tgtcggcatc
     1261 cggctcgcga ttggcataat tgatggcgta gtccaccact cccctgctgg cctcgctccc
     1321 atcgatcgcc acgaccactt tcttcatctg aatccctcct tcgccgcctt ggaaaagaag
     1381 gcggatttcc tcccggtttc accagccatg gaatatagct tcatggctat tatagcacaa
     1441 aatacactat ctagattgaa tttttagttt atttatgtcc ccagtctgtc ccttcccgtt
     1501 tcttttcgcc tcttcttttt gtcttctcca tgctaaaatt acattgaaaa tacccaatat
     1561 ttccggggag gctggtaatg gtgaatcgtt caggattttt ccgtgcggca ctgctctttc
     1621 ttctcttctt cctggcaacg ggttcctgga gcccggtcct ggcccaggat gatgccacaa
     1681 cccacataga gctcatcctg gacgcttcgg ggagcatgtg ggcaaagctc ggggggtcca
     1741 cccggatcga ggtggcgaag gacgccctgg gcaaaatcat cgacgacctg tcggagcgga
     1801 aagggatcgc cgtaggactg cgggtctacg gtcacaggac aaacgactgc aaggacacga
     1861 agctggagat tcccatcggt cccatggacg aaaagaaaat gaaggccttc atcgggaaga
     1921 tcaagcccaa ggggaagacc cccatcgcct attccctcca ggaggcggca aaggacttca
     1981 aaaaggactt cacgggcagc aaggtgatca ttctcgtcac cgacggcctt gaaagctgcg
     2041 gcggcgaccc ctgtgcggcg gcgaaggtcc ttgccgagaa gggaatcgtc tcgaagatcc
     2101 acgtggtggg tttcggaatg gacaagaaat cggtctccaa gctcgagtgc atcgtgaaac
     2161 cctcaggagg gcttctcctg gaggcgaaca gcgccgccga gcttgcgaag gccttcgaca
     2221 ccatagtgaa aactgccctc gatacgaacc tggaggtcac gggacttgac gggaaaggaa
     2281 agcccgtgga gatgtcggtc tccgttctgc aggaagggga tgaagtcctc tcggagaagg
     2341 ggcagactgt aaaggcgaac ctccccaggg aagttacctc gtgcgggcga cagcgacgga
     2401 gacaggcgaa gagatcttct tcgagggtgt ggaccttgtg gaggacaagc tcacctccct
     2461 gaaagccgtg ttttccattg ccaggattcg ggtcagggca ctggacagcg ccgggaagcc
     2521 cgtccgtgcg gagtggcaaa tattccggaa ggacgctccg gaagaaccgg tggtgcgctt
     2581 ctccggcgcc gattggaccg agaaagccct ctcacccggg gaatacaccc tcaaggccca
     2641 ccacaaggac accaaggtca ccctgacggc ggacgcagcc cccgggaacg gggagaccgc
     2701 cgcggtggaa cttgtttttg cccaggggaa gctgctcatt tcgggcctcg actccaacgg
     2761 caagccggtc tacacagact cctacgttta caaagccccc ttcgacaggg acaatcccga
     2821 cgaggcggca cgggacggcg gcaacaaaca tgagtattcc cttgtccccg ggacctacga
     2881 cattctcgtc agggaccaca acacgaaggt cgagcagtgg atcagggacg tggtcatcga
     2941 aggagggaag accgtccgga aggaagtctc cttcgcccag gggaagctga tcatttcggg
     3001 cctcgactcc aacggcaagc cgctctacac cgactcttac gtttacaaag cccccttcga
     3061 cagggacaat cccgacgagg cggggcgtga cggcggcaac agccacgaat attccctcgt
     3121 ccccgggacc tacgacatcc tggtgaggga cggcaacacc aaggtcgagc agtggatcag
     3181 ggacgtggtc atcgaaggag ggaagaccgt ccggaaggaa gtctccttct cccagggaac
     3241 ggtccggctg acggtgaagg gattcgaagg gaaaccagtc tatttcttca cagagatttt
     3301 cctgtccccg gccgaagaaa acgacgaatt cgtccactcg gacagcggcg aaaacgaggt
     3361 atccttcccc atcgtcccgg gcacctacga tgtgaaagtg tggaccgagg agcccagggc
     3421 agagctgtgg gaaaagggaa tggaa
//
LOCUS       DAOTHD010000109         3440 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_32505_length_3440_cov_3.161300, whole genome shotgun sequence.
ACCESSION   DAOTHD010000109 DAOTHD010000000
VERSION     DAOTHD010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32505_length_3440_cov_3.161300"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="P5219_02845"
     CDS             <1..1572
                     /locus_tag="P5219_02845"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HRX25724.1"
                     /translation="KASTDGSVIVSHTCDGWYDHRIRIVPGGEHKPGEMVDIYNIMCY
                     DTRPDRPVKKVGEIPQAEKTYTYFHIGYPFMNDQQLMMGEFTWSGRDELATPEGLLYI
                     ENLEALGLARAATAREAIQVMGELAEKYGYADGGETLVIGDTKELWVFEVCGPGLWNK
                     ESGRPGAHWAARRVPDDQVFVGANRARIGVIDFADTENFMFSTDITKLPEQMGWWKPG
                     TPFHFADIFNPEPYGYPYYQARREWRALNLVAPSQKFDVLGEYDHYPFSVKPDTKLNV
                     RDIMDIYSDHLEGTDYDLTTDAASGPFGNPNRWPTPASVRPEDRKGMDWERPIAMFRC
                     SYSFVSQSRDWLPNPIGGVLWFGQDAPDTTVYVPIYCGVTELPKPWTTGKRAEFDRES
                     AWWAFNLVNNWANLRWDAMYKVIRAKKAEFEDVFFSLQSQAEEKALELYKKDPAEAVA
                     YLTRYTNDNLNKVEKGWWDFAFHLIGKFYDGGMISEEGKMTSPGYPTEYLKKVGYGDL
                     TIRDLERAKSRKAPK"
     gene            1680..2231
                     /locus_tag="P5219_02850"
     CDS             1680..2231
                     /locus_tag="P5219_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HRX25725.1"
                     /translation="MKKNATALFCLCLAALLFAGMASAEAPKLEGPYLVTTCGQSPGA
                     VMLRMSALQAGVQAEHDNALSAPALAGKKAKTLIVTAGTSMKGMGAAGTNVNKEIERC
                     IALMEAARSAGMTVVGAHIEGMARRTDSSDEASIEAVMGAADIILVVEDSDSDGFFTR
                     YAGEHGKPIVKVKDALAIGNALK"
     gene            2305..>3440
                     /locus_tag="P5219_02855"
     CDS             2305..>3440
                     /locus_tag="P5219_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HRX25726.1"
                     /translation="MTMFHHSLLVLMVIVLAFSAGKKFSLTTELSMLAGALAGLAAHM
                     ILPKGADPRSPLLLAEAVRHLVEGTFTYFDVCLIFLTATFFMALYKEAGGVAFIVRKI
                     VRTFHSRRFVCLLLLVLVMLAPGAITGSGATAVLTVGSLVGSVLLAMGVEEDRRTALI
                     FLLAAMSAACPPINLWAMMAAAGANMPYVGFAAPLAVLSISGALFSAFWLAGKGKPVD
                     LEKALSDLPEVPEGWNWYRAGFPFLLLAALVGAGRIIPHGFPVLGLPLIFMICALSVL
                     LISPVRPPVFRVATDTVHNLQGLVGIMVVVGALIQIMTLSGVRGLLSLAVVTLPLTVL
                     FFSLWLILPISEGMLQYAVAPLFGVPLIMLFNMKGLDPVISLAA"
BASE COUNT          727 a         1050 c          934 g          729 t
ORIGIN      
        1 aaggccagca ccgacggatc agtgatcgtc agccatacct gcgacggctg gtacgaccac
       61 aggatcagga ttgttcccgg cggcgagcac aaacccggcg aaatggtgga tatatacaac
      121 atcatgtgct acgacacgag acctgaccgt cccgtgaaga aagtaggcga gatcccccag
      181 gccgaaaaaa cctacacgta ctttcatatc ggatacccct tcatgaacga tcagcagctc
      241 atgatgggcg agttcacatg gagcgggcgg gatgagcttg cgactcccga aggcctctta
      301 tacatcgaaa acctcgaagc cctcggcctt gcccgtgccg ccaccgcccg ggaagcaatt
      361 caggtcatgg gagagcttgc ggaaaaatac ggatacgctg atggcggaga aaccctcgtc
      421 atcggcgaca ccaaggaact ctgggtattc gaggtctgcg gtcccggact ctggaacaag
      481 gaatcaggcc gtcccggcgc ccactgggct gcccgcaggg tcccggacga ccaggtcttc
      541 gtgggagcca accgtgcgag aataggggtc atcgattttg cagacaccga aaacttcatg
      601 ttctccaccg acatcacgaa actccccgag cagatgggct ggtggaaacc cggtacgccc
      661 ttccacttcg ccgatatttt caatcccgaa ccctacggtt acccctatta ccaggccagg
      721 agagagtgga gagccctcaa cctggtggct ccttcccaga agttcgacgt cctcggtgaa
      781 tatgatcatt accccttctc ggtgaagcct gacacaaaac tcaacgtcag ggacattatg
      841 gatatttaca gcgaccacct ggaaggaaca gactatgatc ttacaacgga cgccgcctcc
      901 ggccccttcg ggaacccgaa ccgctggcct accccggctt ccgtccggcc cgaagaccgc
      961 aagggaatgg actgggagcg ccccatcgcc atgttcaggt gttcgtacag cttcgtctcc
     1021 cagagccgcg actggcttcc caaccctatc ggcggtgtgc tctggttcgg acaggacgct
     1081 ccggacacca ccgtgtacgt tcccatttac tgcggagtga ccgaacttcc caagccctgg
     1141 accaccggca agagggcgga attcgaccgt gaaagcgcct ggtgggcttt caacctcgta
     1201 aataactggg caaacctccg gtgggacgcc atgtacaagg taatacgcgc caagaaggcc
     1261 gaatttgagg atgttttctt ttctctccag tcccaggcag aggaaaaagc ccttgagctg
     1321 tacaaaaagg atcccgcaga agccgtggcc tacctaaccc ggtacacgaa cgacaacctg
     1381 aacaaggtgg aaaagggctg gtgggatttc gcgttccacc tgatcgggaa attctacgac
     1441 ggcggtatga tcagcgagga aggcaaaatg acatccccag gctaccccac cgagtacctg
     1501 aagaaagtcg gttatggaga tctcaccatc cgtgacctcg aaagagcaaa gtcgaggaaa
     1561 gcaccgaaat aggcaacaaa ggacactcca ggagcggctc ccccgccgct ctttctcttc
     1621 tttccccgca cgggaacgat accggcgcaa tgaagcgcat caacaggagg ttcagcgaga
     1681 tgaaaaaaaa tgcgactgcc cttttctgcc tttgccttgc ggcccttctt tttgcaggta
     1741 tggcgtccgc agaggctccg aaacttgagg gtccctacct cgtcacgacc tgcggacaga
     1801 gcccgggagc agtcatgctc cgcatgtcgg ctctccaggc aggagtacag gcggaacacg
     1861 acaacgcact ttccgccccg gctcttgcgg gaaaaaaagc gaaaaccctc atcgtgaccg
     1921 ccggaacaag catgaaaggt atgggagctg cgggaacgaa cgtcaacaag gagatagaac
     1981 ggtgcatcgc cctcatggaa gctgcccgtt ccgccggaat gaccgtcgtg ggggcccaca
     2041 tcgaaggcat ggcccggcgg acggacagtt cggatgaagc atccatcgaa gccgtcatgg
     2101 gagctgccga catcatcctc gtagtggagg acagtgacag cgacggattt ttcacacggt
     2161 acgccggtga acacgggaaa cccatcgtga aggtcaaaga tgccctcgcc atcggaaacg
     2221 ctctgaaata agcggctttc gtcagggttg gagggcctct caacggggcc ctttccccat
     2281 ttttcttgaa aggatgtatc accgatgaca atgtttcatc attcgcttct cgttctcatg
     2341 gtcatcgttc tggcgttttc cgcagggaaa aagttctccc tgaccaccga actttccatg
     2401 cttgccgggg cccttgcagg tcttgccgcc cacatgatcc tccccaaggg ggcagatccc
     2461 cggtcccctc tcctcctcgc agaagcggta cggcatctcg tcgaagggac cttcacctac
     2521 ttcgacgtct gtctcatctt ccttacggcc acgtttttca tggccctcta caaggaagca
     2581 gggggcgtgg ccttcatcgt gagaaaaata gtccggacgt tccattcccg ccggtttgtc
     2641 tgtcttcttc tcctggtcct cgtaatgctt gctcccggag ccatcacggg ctccggagcc
     2701 acggccgtgc tgaccgtagg ctcccttgtc gggtccgtcc tccttgcaat gggcgtggag
     2761 gaagaccgtc gcaccgccct gatcttcctt cttgcggcaa tgagcgcagc ctgtccgccc
     2821 atcaatctgt gggcaatgat ggccgccgcc ggtgccaata tgccctacgt cggttttgcc
     2881 gctcctcttg ccgtactctc catttcgggt gccctttttt ctgccttctg gcttgcggga
     2941 aaaggaaagc ccgttgatct tgagaaagcc ctttctgacc ttcctgaagt tccggagggg
     3001 tggaactggt atcgcgcagg gtttcccttt ctcctgcttg cagctctcgt gggggcagga
     3061 cggatcattc cccacggttt tccggtcctg ggactcccgc tgattttcat gatctgcgcc
     3121 ctttccgtcc ttttgatcag cccggtgcgt cctcccgtct tccgggttgc taccgatact
     3181 gtacacaacc tgcagggcct cgtgggcatc atggtcgttg tcggagccct catccagatc
     3241 atgaccctga gcggcgtcag gggactgctt tccctggcgg tggtcactct gccgctgacg
     3301 gttctcttct tctctctttg gctcatcctg cccatttcgg agggaatgct ccagtatgcc
     3361 gtcgctccgc ttttcggtgt gccgctgatc atgctgttca acatgaaggg acttgacccg
     3421 gtgatttccc tcgcggcatg
//
LOCUS       DAOTHD010000110         3426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_32692_length_3426_cov_3.132305, whole genome shotgun sequence.
ACCESSION   DAOTHD010000110 DAOTHD010000000
VERSION     DAOTHD010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32692_length_3426_cov_3.132305"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="P5219_02860"
     CDS             complement(<1..361)
                     /locus_tag="P5219_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRX25727.1"
                     /translation="MNLHQLRIFCAVVEEGSFRQAAGKLFLSQPSVSQHVASLEKDHS
                     IRLFERKGRTISLTPEGRALYVLATDLLRQADEIPARFRDLQALRSGRLAAGVSPFAG
                     YYILPPALADFRAAFPSV"
     gene            481..1725
                     /locus_tag="P5219_02865"
     CDS             481..1725
                     /locus_tag="P5219_02865"
                     /inference="COORDINATES: protein motif:HMM:NF009555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HRX25728.1"
                     /translation="MEGKRQEDLIQLCRELLRRPAVSGREGEVARFAADVMTALGYDL
                     VTTDSYGNVVGSMTFSGEGERVLLTSQMDHVDVGDAAEWSKYPYGAFIEDGRIYGRAA
                     SDQKGALSAMIMAGAFLRADRGHSLKGQLSVAAAVHQETFENVASRAIADTVDPTCVI
                     VGEASSLLLERGQRGRAEIRLDTFGKMAHSSHPEYGINAADTMNALLAFLKQHFAPPR
                     DPFLGEGILVLTSLYTSPAGTGGAVPEKCTALFDRRLLRGETLEGAAGELRNLISRAA
                     DQIPGLRARVSFPVMEDRCYTGAPIRGNHYAPAWALPEESLFLKVLSDALAEEGLPCR
                     ISDRPGFGTNGCHFAVERKVPTVIYGPSRRELVHGVDEYIEIRELLGACRGYYAMAGR
                     LLSGRGGVQIPAEGAAVSEGGR"
     gene            1727..2974
                     /locus_tag="P5219_02870"
     CDS             1727..2974
                     /locus_tag="P5219_02870"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRX25729.1"
                     /translation="MGKPVELRCVLCGASYAPGEVEYTCPKCGLDGTLDVLYDYEEAA
                     RTLTSGTLSGRASSLWRYGEILPVEAEENIPSLSVGWTPYYDCSALAEEYGVREFFVK
                     DDGRNPTGSLKDRASAVGVARAIELGQRTVACASTGNAASSLSGFAAVGGLKSFIFVP
                     EKAPAAKVTQLLVYGATVVLVRGDYADAFRLATAAIEKYGWYNRNCAINPYLVEGKKT
                     CALEIAEQSGWNPPDRVFISVGDGCCIGGLYKGFSDLMKIGLISRMPKITGVQAEGAK
                     PILDAFQNGADRVVFGPADTVADSISVGAPRNWAKALRAVRESGGDMVAVSDAEILSA
                     IPELARKTGVFGEPAGAAAFAGFRKMAGEGRLGRKERVAVVVTGNGLKDIESARKSAG
                     SPIVSEPDIDRFSEQLERSGFPC"
     gene            2968..>3426
                     /locus_tag="P5219_02875"
     CDS             2968..>3426
                     /locus_tag="P5219_02875"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661101.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRX25730.1"
                     /translation="MLEKGQEYLRDADPEIFSAITDELERQRNGIELIASENFVSRAV
                     LAAMGSVMTNKYAEGYPHARYYGGCHVVDRAEDLARDRAKELFGCDHVNVQPHSGSQA
                     NMAVYFSVLQPGDTILAMNLAHGGHLTHGSPVNFSGKLYNIIPYGVDKTTE"
BASE COUNT          674 a         1012 c         1134 g          606 t
ORIGIN      
        1 agacggacgg aaaagcggca cggaaatcgg ccagggccgg gggaagaatg tagtagcccg
       61 cgaaggggga gacgcccgcg gcaagcctgc ccgagcgcag ggcctgcagg tcccggaacc
      121 tggcagggat ctcgtcggcc tggcggagga gatccgtggc aagaacatag agagccctgc
      181 cctccggggt gagagagatg gtccgtccct tccgttcgaa aaggcggatg ctgtggtcct
      241 tttcgaggga cgcgacatgc tggctcaccg acggctggga gaggaaaagc ttcccggctg
      301 cctgccggaa ggaaccttcc tccacgacgg cgcagaagat tctcagctga tggaggttca
      361 catcgatccg cctcccctgg tttgatatag gaaatactga tggattatat cattatttct
      421 ttcttgaaaa ttatactgtc tggtgtatct tggagacgtt ggagaaggga gcgtcgagcc
      481 atggaaggga aacgacagga agatctcatc cagctctgca gggagctcct ccgccgcccc
      541 gccgtgtccg gcagggaagg ggaggtggcc aggttcgcgg ccgacgtcat gaccgctctc
      601 ggctatgacc tggtgaccac cgactcctac ggcaacgtgg tgggttccat gacgttctcc
      661 ggcgaaggtg aaagggttct gctcacttcc cagatggatc acgtggacgt gggggacgcc
      721 gccgagtggt cgaagtatcc ctacggcgcc ttcatcgagg acggcaggat ttacggaagg
      781 gccgcttcgg atcagaaagg ggccctctcc gccatgatca tggcgggcgc ctttctcagg
      841 gccgaccggg ggcattccct gaaaggacag ctttccgtgg ctgccgccgt ccaccaggag
      901 actttcgaga acgtggcctc cagggccatc gccgacacgg tggacccgac ctgcgtcatc
      961 gtgggggagg cgtcctccct tctgctggag cggggacagc ggggcagggc ggaaatccgg
     1021 ctggacacct tcgggaaaat ggcccactcc tcccatcctg aatacggcat caatgccgcc
     1081 gacaccatga acgcccttct cgcctttctg aaacagcatt ttgcccctcc ccgggatcct
     1141 tttctcggcg agggaatcct tgtgctcacc agcctttata catcccctgc cggaacgggc
     1201 ggcgccgtcc cggaaaaatg caccgccctc ttcgaccgga ggcttctccg gggggaaacc
     1261 ctcgaagggg ccgccgggga actgcggaac ctcatctccc gcgccgcgga tcagatacct
     1321 ggcctccggg caagggtctc ctttccggtg atggaggacc ggtgctacac cggggccccc
     1381 attcggggaa accattacgc tccggcctgg gccctgccgg aggaatcgct ctttctgaag
     1441 gttctttcag acgctcttgc ggaagagggg cttccctgcc ggatctccga caggccggga
     1501 ttcgggacga acggatgcca ctttgccgtg gagagaaaag tgccgacggt catatacggt
     1561 ccatcccgga gggaacttgt ccacggagtg gatgaatata tcgaaatacg ggaactcctt
     1621 ggagcctgcc gggggtatta cgccatggcg ggcaggctcc tttccggacg gggcggcgtg
     1681 cagatcccgg cggagggagc agcggtttcg gaaggaggaa gataaaatgg gaaaaccggt
     1741 ggagctgcgg tgcgtcctct gcggggcgtc ctacgcgccg ggggaggtgg agtacacctg
     1801 cccgaaatgc ggactcgacg gaaccctcga cgtgctctac gactacgagg aggccgcccg
     1861 cacattgacg tccggtaccc tgtcggggag agcatcatcc ctgtggcgct acggcgaaat
     1921 cctccccgtg gaggctgagg agaatattcc gtccctctcg gtgggatgga ctccctatta
     1981 cgactgttcc gcccttgcgg aggagtacgg cgtgcgggag tttttcgtga aggacgacgg
     2041 acggaatccc acgggctccc tcaaggaccg ggcgagcgcc gtgggtgtgg cccgggcgat
     2101 tgagctcggt cagcggaccg tggcgtgcgc ctcgacgggg aatgccgcca gctccctctc
     2161 gggattcgcc gcagtgggag ggttgaagag cttcatcttt gttccggaga aggctccggc
     2221 ggccaaggtg acccagctgc tcgtctacgg ggcgaccgtg gtgctggtcc ggggagatta
     2281 tgccgacgcc ttccggctgg ccacggcagc catcgaaaaa tacgggtggt acaaccggaa
     2341 ctgcgccatc aacccctacc tcgtggaggg caagaagacc tgcgctctcg agatcgccga
     2401 acagagcgga tggaatcctc cggacagggt cttcatctcc gtgggggacg gctgctgcat
     2461 aggcgggctg tacaagggat tcagcgacct gatgaagatt gggctcatca gcaggatgcc
     2521 gaaaattacg ggagtccagg cggagggtgc gaagcccatc ctcgacgcct ttcagaacgg
     2581 tgccgaccgg gtggtgttcg gccctgcgga caccgttgcg gacagcattt ccgtcggcgc
     2641 ccccaggaac tgggcaaagg ccctcagggc ggtgagggaa tcgggaggag acatggttgc
     2701 cgtgtcggac gccgaaattc tgtccgccat ccccgagctg gcgagaaaga ccggtgtctt
     2761 cggggagccc gccggggcgg cagctttcgc cggattcaga aagatggcgg gcgaaggccg
     2821 cctgggaaga aaggaacggg ttgccgtggt ggtcacaggc aacggcctga aggatatcga
     2881 gagcgcgcgg aaatccgccg gctctcccat agtatcggag cctgacatcg acaggttctc
     2941 tgaacaactt gaaaggagtg ggttcccgtg ttagagaaag gccaggagta tcttcgcgac
     3001 gccgaccccg aaattttcag cgccatcacc gacgaactcg agaggcagag gaacggcatc
     3061 gaactcatag catcggaaaa tttcgtctcc cgggctgtcc tcgccgccat ggggtcggtt
     3121 atgaccaaca agtatgcgga agggtacccc catgcccggt attacggcgg ctgccacgtg
     3181 gtggacaggg cggaggacct cgccagggac agggcgaagg agctcttcgg ctgcgaccac
     3241 gtgaatgtcc agccccactc cgggtcccag gcgaacatgg ccgtctactt ctccgttctc
     3301 cagccgggcg acaccatcct ggccatgaac ctcgcccacg gagggcatct cacccacggc
     3361 tccccggtca atttctcggg caagctctac aacataatcc cctacggggt cgacaagacc
     3421 accgag
//
LOCUS       DAOTHD010000111         3421 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_32749_length_3421_cov_3.026144, whole genome shotgun sequence.
ACCESSION   DAOTHD010000111 DAOTHD010000000
VERSION     DAOTHD010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32749_length_3421_cov_3.026144"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="P5219_02880"
     CDS             <1..900
                     /locus_tag="P5219_02880"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712825.1"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HRX25731.1"
                     /translation="YGLKLDVVNPVVDPTFSFMSVDWDGKIRMDCSSPYAMAGLVNLK
                     DRYDIAFGNDTDFDRHGIVVPDRGLLAPNGYLAVAVDYLFTHRPGWRKDAAVGKTLVS
                     SSLIDRVARSVRRKCVEVPVGFKWFVEGLLDGSLGFGGEESAGASFLRKDGTAWSTDK
                     DGIILTLLAAEILAVTGEHPGIRYDGITARLGVPYYARIDAAAGPERRKLLKKLTPEA
                     AEGDTLAGEPILAKLTEAPGNGAPIGGLKVTSENGWFAARPSGTEDVYKIYAESFLGE
                     DHLRRIQEEARDMVDGVFARAGV"
     gene            complement(966..1157)
                     /locus_tag="P5219_02885"
     CDS             complement(966..1157)
                     /locus_tag="P5219_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25732.1"
                     /translation="MEKMESTWVSPVLSVLSAQGGRVTGKAAKERPKQNKKRFREYLH
                     PDSREGSDSETEDRLDTLA"
     gene            complement(1604..2812)
                     /locus_tag="P5219_02890"
     CDS             complement(1604..2812)
                     /locus_tag="P5219_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HRX25733.1"
                     /translation="MNLPLLKQWFRELEPVGADPDGGVTRLAYSAEEDAMFEKVAGFA
                     SGLGFSVTEDCIGNMFISFPGETDRPCRVVGSHLDSVPQGGRYDGVAGVLAGLLVMEK
                     IRRLGLPLPVKTAVFRAEESSLYGLATAGSGTVAGSICTDRLKDAKSLSGDSLYDAMI
                     RKGHSPGTCGLDPMIDFTELHIEQGRVLWEAKEPLGIVTAIAAPTRLKVTIHGRQDHS
                     GATPMNLRKDGLCAAAEIVLGVEEAGKAESLHATVATVGVAHVHPNALNVVPGMVELR
                     IDIRGIIKESISRAVASVRNIIRETEGRRSIRCDVDIISAMDPVTLAPCVTDSLAAAA
                     EESGIPFRRMPSGAGHDAMKIAAVAPAGMVFIPCREGISHNPAEEASLKDVALGADII
                     LESMRIRSRP"
     gene            2936..3283
                     /locus_tag="P5219_02895"
     CDS             2936..3283
                     /locus_tag="P5219_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25734.1"
                     /translation="MKKFVLLLFALAVAFSAGGAAAHPPSDLDLLYSREEGVLEVYAP
                     HSVPDGKRHYINLFTLSLNGDLMYRLEPAWQVDGKAAAASFHVGDLPKGAVLEVEAVC
                     NRAGNLKKTLTVE"
BASE COUNT          700 a          996 c          997 g          728 t
ORIGIN      
        1 tacgggctga aactggacgt ggtcaacccc gtggtggatc ccaccttttc gttcatgtcc
       61 gtggactggg acggcaagat ccgaatggac tgctcatccc cctatgccat ggcgggcctg
      121 gtcaacctga aggacaggta cgacatcgcc ttcggcaacg acacggactt cgaccgccac
      181 ggaatcgtgg tccccgaccg gggacttctt gccccgaacg gctacctggc cgtggcggtg
      241 gactacctct tcacccacag gcccggctgg aggaaggacg cggccgtggg aaagaccctc
      301 gtatcaagct cgctcatcga cagggtcgcc cggtcggtga gacggaaatg cgtggaggtt
      361 cctgtcggct tcaaatggtt cgtggaagga ctgctcgacg gctccctggg cttcgggggc
      421 gaggaaagcg ccggggcgtc cttcctcagg aaggacggga cggcgtggtc caccgacaag
      481 gacggaatca tcctcaccct cctcgcggcg gaaatcctgg cggttaccgg agagcacccc
      541 ggaatacggt atgacgggat cacggcgaga ctcggcgttc cctactatgc aaggatcgat
      601 gccgccgccg ggcctgagcg gagaaagctg ctcaagaagc ttacccccga ggctgcggag
      661 ggagacaccc tggccggcga gcccattctc gccaagctga cggaggctcc cggtaacggg
      721 gcacccatcg gcggcctgaa ggtgacttcc gaaaacgggt ggtttgccgc ccggccctcc
      781 ggcactgagg acgtgtacaa gatttacgcc gagagcttcc tcggcgaaga ccacctcagg
      841 aggatccagg aagaggcccg ggacatggtg gacggggtct ttgccagggc aggcgtctga
      901 caccggggaa ggaaagcggg gccgcccctc aggggcggcc ccgctcttca ttgatggcag
      961 tgagctcacg caagggtatc gagccgatct tcggtctccg aatccgaccc ttccctgctg
     1021 tcggggtgca ggtactccct gaaacgtttc ttgttctgtt tcggtcgctc ctttgccgcc
     1081 tttccggtga ctcgtcctcc ctgggccgaa agaacggaaa gaacgggaga cacccacgtg
     1141 gattccattt tttccatggc gctccctcct ttgtcttttc ttatcggcgt ttttagaata
     1201 atccattaac aaaaagccgc cccttctggg ggcggcagga agaaaacgat gtcaggcttc
     1261 gtcctttgca ttgctcttca ttttcatgac gaccatgacg atgccgatga cggccataat
     1321 cccaagccaa accatggtga tgttattcat ccatttcacc tccttacatc caggagcacg
     1381 acgaattcgc tctgcaaagc gcggcaataa gtgaaaagaa aaacggaaaa cgaagatctg
     1441 tgaactgaaa aacgaataga aagaagatcg aaactattgg actgaaatta acctaccata
     1501 attggcagct caattcaacc caagattgaa aaaaacaggc aatttgctat tccctatttt
     1561 cagacctatt tcctgaggga atggatggtg ctcaatgttt cccctacggt ctgcttcgga
     1621 ttcgcatgct ctccagtatg atgtctgctc caagggcaac gtccttcagg gatgcctctt
     1681 ccgccggatt gtggctgatt ccttccctgc aggggataaa aaccatgcct gcgggagcga
     1741 cggcggcaat cttcatggcg tcatgtcctg ctccgctggg cattctccgg aaggggattc
     1801 ccgactcttc cgccgccgcc gcaagtgaat cggttacgca gggggccagg gtaactggat
     1861 ccatggcgga gatgatgtcc acgtcgcacc ggatacttcg ccggccttcc gtttcacgga
     1921 tgatatttct gacggaggcg acagcccggg agatgctttc ttttatgatt ccccggatgt
     1981 cgatcctcag ctccaccatg cccggaacca cgttcagggc gttcggatgg acatgggcaa
     2041 cgcccaccgt ggccactgtt gcgtgaaggc tttccgcttt tcccgcttct tcgacgccga
     2101 gaacgatttc cgccgctgcg cagaggccgt ctttcctcaa gttcatgggc gtggctcctg
     2161 aatggtcctg ccggccgtgg atcgtgactt tcagccgcgt aggggcggca atggcggtca
     2221 cgataccgag aggttccttc gcctcccaga gcacccggcc ctgttcgatg tggagttcgg
     2281 taaagtcgat catggggtcg agaccgcagg taccgggaga atgccctttt cgtatcatgg
     2341 cgtcgtacag gctgtcgccg gaaagggatt tcgcgtcttt gagccggtcg gtgcagatgc
     2401 tcccggcgac agttccgctg cctgccgtgg caagtccgta caggcttgat tcttcggccc
     2461 ggaaaacggc ggtttttacg gggagaggaa gcccgagcct ccggattttt tccatgacga
     2521 gcaggccggc aaggacgccg gcaaccccat catatcgccc tccctgcggg acggagtcga
     2581 ggtgggatcc taccacgcgg cacggacggt ccgtttcgcc cggaaaggag atgaacatat
     2641 tccctatgca gtcctctgtg acggaaaaac cgaggccgga ggcaaagccc gcaacctttt
     2701 caaacatggc atcctcctcg gccgaatagg caagcctggt gacacctccg tcgggatcgg
     2761 ctccaacagg ctccagttcc ctgaaccatt gtttcagcag cggcagattc attccatcat
     2821 ctccagcggt gcccgttcac ctggggcgaa ggggacagga tattgaaaaa cgttttttct
     2881 gctacactat tctatgaaag gtttcatatt ccagaactga aggagagtga gtacagtgaa
     2941 aaagtttgtt cttctgctgt ttgcactggc tgttgccttt tccgcgggtg gggcggctgc
     3001 ccatcccccc tcggaccttg atcttctcta cagcagggag gaaggcgttc tcgaggttta
     3061 tgcaccccac tcggttcctg acgggaaaag acactatatc aacctcttca ccctttccct
     3121 caacggggat ctcatgtacc gcctcgagcc ggcctggcag gtggacggca aggccgctgc
     3181 tgccagcttc cacgtgggag atctccccaa gggagcggtg ctcgaggtcg aagcggtgtg
     3241 caatcgcgcg ggaaatctga aaaaaaccct caccgtggaa taggaaacaa aatcgaggac
     3301 gtccttcggg gcgtcctcgc tgcatgtccg aatatggggc gtgatttacg ccgtcagcga
     3361 cagttccgtg tcccgccggc cgaaagttcc tgtttttgcg tctgagtagg cacgtacggc
     3421 g
//
LOCUS       DAOTHD010000112         3408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_32907_length_3408_cov_2.903967, whole genome shotgun sequence.
ACCESSION   DAOTHD010000112 DAOTHD010000000
VERSION     DAOTHD010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32907_length_3408_cov_2.903967"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="P5219_02900"
     CDS             <1..334
                     /locus_tag="P5219_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HRX25735.1"
                     /translation="IVVLAVWMSMAATDIIGKVWAIFFPIMTFVAAGFEHSIANMYFM
                     SLGLLLKGTPAAVSASGLSEQAMASVSLGGFFGNLIPVTIGNMVGGILFVAVFYYFIF
                     KDGLTDLE"
     gene            662..1642
                     /locus_tag="P5219_02905"
     CDS             662..1642
                     /locus_tag="P5219_02905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRX25736.1"
                     /translation="MSGGIIEPFWHPAVSEGFILDWDGVLAETRLNFAPLRARYFDGK
                     FVPLFEALETLPPSIADRLRKDIYDLEMAGAESAVAVEGARELLEWLEGEDIPWCVVS
                     RNCMDSIILAAERAGLPLPPVVRSRDEPPVKPAPEALWSAAEQIGVPSGKCVMVGDFV
                     YDLVGARRAGMRAVLVQRPDAEWKHWADVSFDRLSEFVQSLKNPKAIVPWEYAPLVSE
                     KGENSLLAGRGKGTVLSSKTPDVLLRLMKKAEEGILFFRLDDPDAPLLPGQWRKLPGL
                     SPAWLDRPLKAAAEYVLGTRFPFAALVAEESSPDGVEWETISAPEGGIGE"
     gene            1639..2922
                     /locus_tag="P5219_02910"
     CDS             1639..2922
                     /locus_tag="P5219_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRX25737.1"
                     /translation="MTPSATNRWEVPLAERMRPKTLDEFTGQGHLIGPGAPLRKLLES
                     NRTPSCILYGPPGVGKTTLVRLMASETGRDILEINAVSAKVSELRDLVSEAANLKAMR
                     AGHAAIAFVDEIYHFNKSQQNALLPSVEKGDLILVGTTTENPWFEINKTLLSRMVVFQ
                     LTPLSEEDLSILLKRALADRDRGLGEFEIEAEEKALRRIASMAGGDARQALTRLEFIA
                     ASVAARGGTILTEDGVLSALPLAAIRFDKASEDHFAVISALIKSIRGSDPDAAVYWLA
                     RLLAGGENLRFIGRRLLISAAEDVGLADPNALTVAVSAVQAVEMTGLPEARIILAEAV
                     IYLAAAPKSNSAYLAVDSAAKAIEKGDLQEVPFHLRPDGSGYVYPHDDPRHWVPQQYM
                     DRQRRFYFPGTLGIEGNMSSRLRKFWRRFSEGGDS"
     gene            complement(2925..>3408)
                     /locus_tag="P5219_02915"
     CDS             complement(2925..>3408)
                     /locus_tag="P5219_02915"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRX25738.1"
                     /translation="AMSPETLQRFVAGASAARKIGASLLYSGGYPEKAAEGRIEEMVR
                     RTAERIGFSGELLVEGRSRTTWENFLFSSEIIRERGFDEVILVTSGFHLRRSMNSASR
                     FLWSVPVRPLSSGRLEGDGPFIATDLLPSPSGLRNTSLALRELAGIFAYNLFGSLKDR
                     "
BASE COUNT          717 a          983 c          990 g          718 t
ORIGIN      
        1 gatcgtcgtt ctcgcggttt ggatgtccat ggcggccaca gacatcatcg ggaaggtctg
       61 ggcaattttc ttccccatca tgaccttcgt tgcggccggt tttgagcact ccatcgccaa
      121 catgtacttc atgtctctcg gtcttcttct caagggcaca ccggcagcgg tcagcgcttc
      181 cggactttcc gagcaggcaa tggccagcgt ctctctcggc gggttcttcg gcaacctcat
      241 tcccgttacg atagggaaca tggtcggcgg aatcctgttc gtcgcggttt tctactattt
      301 tatattcaaa gacggactga cggacctcga ataaagacag gcctgggcat cttcccggag
      361 gggcgtccct aaaggcgtcc ctcctttttt atatggtata atatgcgtga agaaagaaga
      421 caataccctg cgatgcccgt gatcgtgata tttgtcttga gccaacactc gagccttgcg
      481 gggctgacag tttgggccgg tgatggaaat gctccggcat tgacggaact gaggctcaac
      541 aggccccaaa cgttaaggac cgggtcaaga cgttcatgac acgacattgc gggggaggta
      601 gatacagggg cggggaagca gcaccctcgc ccctgtcttg tgcaaaaaag gaggcgcacc
      661 catgtccgga ggcatcatcg agccgttctg gcaccccgct gtttccgagg ggtttatcct
      721 cgactgggac ggtgttctcg ctgaaacgcg gctgaatttc gcacctctgc gggccaggta
      781 tttcgacgga aagttcgtgc cccttttcga agcgttggaa acccttccgc cgtctattgc
      841 tgatcggctc cgaaaggaca tttatgacct ggaaatggca ggcgctgaat ctgcggttgc
      901 cgtggaggga gcccgtgaac tgctcgagtg gctcgaagga gaggatattc catggtgcgt
      961 tgtctcacgg aactgcatgg actccataat cctcgccgct gaaagggcag ggctgcccct
     1021 tcccccggtg gtccggagcc gtgatgagcc gccggtgaag ccggcccccg aggcactctg
     1081 gtccgcggcg gaacagatcg gtgttccttc agggaaatgc gtcatggtgg gggattttgt
     1141 ctacgacctc gtcggtgcgc gccgcgccgg aatgagggcc gtgctcgtcc agcggcctga
     1201 tgcggagtgg aagcactggg ccgatgtttc tttcgaccgc ctcagcgagt tcgttcaatc
     1261 ccttaaaaat ccgaaggcca ttgttccgtg ggaatatgcc cctcttgttt cggagaaagg
     1321 agaaaacagc ctcctcgcgg gacgggggaa agggacagtc ctctcatcga aaactccgga
     1381 cgttcttctc cgtctcatga aaaaagctga agaaggaata ctttttttcc gtcttgacga
     1441 ccctgacgcc cctctcctcc cgggacagtg gagaaaactg cccggccttt ctccggcctg
     1501 gctggaccgg cccttgaagg ctgcggctga gtacgttctg ggaacacgct ttccctttgc
     1561 cgcccttgtg gctgaagaat cgtccccgga cggtgtagaa tgggaaacca tttcagcacc
     1621 tgagggagga ataggggaat gacaccttcg gcaacaaaca ggtgggaagt ccccttggcg
     1681 gaacggatgc gcccgaaaac cctcgacgag ttcacgggac aggggcatct tatcggtccc
     1741 ggggctccgc tccggaaact cctcgagtca aaccggaccc cgagctgcat tttgtacggc
     1801 cctcccggag tcggaaagac gaccctggtc aggctcatgg cttcggaaac gggacgagac
     1861 atcctggaga tcaatgctgt ctccgcaaaa gtttccgagc tccgggatct cgtgtcggag
     1921 gcggcgaatc tcaaggccat gagggcggga cacgctgcca tagcctttgt ggacgagata
     1981 taccacttca acaagagcca gcagaacgcc ctcctgccat cagtggagaa gggggacctt
     2041 atcctcgtgg gcaccactac agaaaacccc tggtttgaaa tcaacaagac ccttctttcc
     2101 aggatggtgg ttttccagct cacccccctt tccgaagaag atctctcaat cctgttgaag
     2161 cgggctcttg cggaccggga caggggcctt ggggaatttg aaatcgaggc ggaggaaaaa
     2221 gccctgcggc gaattgcctc catggcaggg ggagacgccc ggcaggccct cacgaggctt
     2281 gaattcatcg ccgcttccgt cgccgcgcgg ggcggaacca tccttacaga agatggcgtt
     2341 ctgtcagccc ttcccctggc agccatccgg tttgacaaag cctccgaaga ccattttgca
     2401 gtgatctccg ccctgattaa aagcattcgc ggatccgacc ccgatgccgc cgtctactgg
     2461 cttgcacgcc tgctcgccgg aggggaaaat ctccggttta tcggcaggcg ccttctcatt
     2521 tccgccgcgg aagacgttgg ccttgcagac cccaatgccc tcacagttgc ggtctccgca
     2581 gtccaggccg ttgaaatgac aggcctgccc gaagcgagga taatactcgc cgaagcggtc
     2641 atctatctcg cggcggctcc gaagagcaac agcgcctatc tagccgtcga cagcgccgcc
     2701 aaggccatcg aaaagggaga tcttcaggaa gtcccctttc acctcaggcc cgacggttcg
     2761 gggtatgttt acccccacga cgacccaagg cactgggtgc cccagcagta tatggacagg
     2821 cagcgcaggt tttattttcc gggaacgctg gggattgagg ggaatatgtc atcccgcctg
     2881 cggaaattct ggaggcggtt cagtgaaggg ggagactcct aggcttaccg gtctttcaga
     2941 cttccgaaaa gattgtaggc aaagatgccc gcaagctccc gaagggcaag ggatgtattt
     3001 ctcagccctg aaggagaggg gagaaggtcg gtggcgatga acggaccgtc cccctcgagg
     3061 cgtcctgaag aaaggggccg caccgggacg gaccagagaa accgggacgc tgaattcatg
     3121 gatctgcgaa ggtgaaatcc tgacgttacc agaatgactt catcaaaacc tctctccctg
     3181 attatctccg acgagaaaag aaaattctcc caggtcgttc gtgaccgccc ttccacaaga
     3241 agttcgcctg agaagccgat tctctccgcc gtccgccgta ccatttcttc aatccgccct
     3301 tctgccgcct tttccggata ccctcctgaa tagagaaggg aagcaccgat tttccgggcg
     3361 gctgaggctc ctgcgacaaa tcgctgaagt gtttccgggc tcatggcg
//
LOCUS       DAOTHD010000113         3402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_32990_length_3402_cov_2.307439, whole genome shotgun sequence.
ACCESSION   DAOTHD010000113 DAOTHD010000000
VERSION     DAOTHD010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32990_length_3402_cov_2.307439"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="P5219_02920"
     CDS             <1..223
                     /locus_tag="P5219_02920"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_133957783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRX25739.1"
                     /translation="MLGYRFSRGWSIILASVLFGLAHLASPHAPFAVLAFFPGLAMGY
                     LRDRHGSVLPAILYHWIGNIWSIWLYPRF"
     gene            250..1317
                     /locus_tag="P5219_02925"
     CDS             250..1317
                     /locus_tag="P5219_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25740.1"
                     /translation="MKSIRSMIIPAVVSVIAAFLFSSPVYAGPVYRIVMPLVRGEEAR
                     AELPGGRIFPLGKVTGLPTATRWPGYTASKWAAPGTVAASAVNAVHLTLSVEKDAGRT
                     MSILPLHTVAPAAGEKAFITLDVPAGTGFFGGWAPPVGTRVLVRKADGLLVPLEVSGL
                     PSPGDELIFDVEEEERPYIIDVENRPGGRVLGWYPAGPKVLARVIRPVRGVGRFGGTE
                     FQGRGRIRANHSGVIDVSTAGRGVTGGFQILPFLHSKSKEMGGAWQLTQWLIIASETE
                     RALPGTAPLFLGNLVPGSQMSDVLWDIWSTYGRKPLVLCRLDGGPWVNLPEEGGRQDF
                     ALERVTHLRIYSPFTGEPQKQ"
     gene            complement(1376..3214)
                     /gene="glmS"
                     /locus_tag="P5219_02930"
     CDS             complement(1376..3214)
                     /gene="glmS"
                     /locus_tag="P5219_02930"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRX25741.1"
                     /translation="MCGIVGYIGERNVKDVLINGMRSLEYRGYDSAGVALMDAPELHI
                     VKGVGKVADLEKILEDHPVSGSQGIGHTRWATHGGVTEVNAHPHSDLSSSLVLVHNGI
                     VENYREIRRELAEGACGDYKSETDTEVVAELLSRLYSARGSMVEALVDLYARLSGSFA
                     LVIMAREAPGKIYCLRKGSPLVVGASPKGETLCASDVPAILPYTQDVVYLDDGDIAEL
                     SSGGILFWNASGETLEKIPTHIDWDVTMVDKGGYPHFMLKEINEQGAVLRNSMAKRLT
                     EGTVDLSAELPWTKEDVASWKRLHIIACGTSYYAALVAERFLETITDFDIRADVASEY
                     RYRNIPLGPDTLAVFVSQSGETADTLAAERIAREKGARCLAVTNVIGSTIAREVHDVL
                     QLKAGPEIGVAATKTFMGQMAVLYLLAIHLAKMQGKLSPEQERKYCDELTALPYKLES
                     VLQRQESLQETAMLYNEARDFLFLGRGISYPVALEGALKLKEISYVHAEAYAAGEMKH
                     GPIALLDPRVPVVVISPKDGLHEKTFSNILEAKARKSPVIAIATEGDDSLDGTADHIF
                     FVPETLDQFTPFLTVAPLQLFSYFFARFLGCHIDQPRNLAKSVTVE"
BASE COUNT          612 a         1016 c         1040 g          734 t
ORIGIN      
        1 catgcttgga taccgttttt cccgggggtg gagcattatc ctggcctccg ttcttttcgg
       61 tcttgcccac cttgcctctc cccacgcccc cttcgcggtg ctggctttct ttccgggact
      121 ggccatggga tacctccggg accgccacgg gtcggtgctg ccggcaattc tctaccactg
      181 gataggcaat atctggtcta tctggctgta tcctcgtttc tgaggaatac gaagaaagga
      241 gtttttccca tgaaatccat tcgttccatg atcattccgg ctgttgtgtc cgtcattgcg
      301 gcctttctct tctcttctcc cgtgtacgca gggcccgtct acaggatcgt gatgcccctc
      361 gtccggggag aggaggcgcg ggcggaactc cccggcggac gcatttttcc cctggggaaa
      421 gtgactgggc tgcctacggc aacccggtgg ccggggtata cggcatcgaa atgggccgca
      481 ccgggaacgg tggctgcttc ggcggtgaat gccgttcatc tcaccctgtc ggtggagaag
      541 gacgccggaa ggaccatgag catcctgccg ctccacacgg tggctccggc ggccggagag
      601 aaagctttca tcaccctcga cgttccggcg ggcaccggtt tcttcggagg ctgggctcct
      661 cccgtgggga caagggtcct ggttcggaag gcagatggac tgctggttcc cctggaggtc
      721 agcggtcttc cctctccggg agacgaactg atttttgacg tggaggaaga agaacggccc
      781 tacatcatcg acgtggagaa ccgccccgga gggcgggttc tgggatggta tccggcggga
      841 ccgaaagtcc ttgcccgggt tatccgcccg gtgaggggcg tcgggaggtt cggggggacg
      901 gagtttcagg gcaggggaag gatcagggcg aatcattcgg gagtcatcga cgtgagcacc
      961 gccggccggg gggtgaccgg aggattccag atacttccct tcctccactc gaagtcaaag
     1021 gagatgggag gagcctggca gttgacccag tggctcatca tcgcctccga aacggagcgg
     1081 gcattgcccg gaacggcgcc tctgttcctg ggaaacctcg ttccgggaag ccagatgtcg
     1141 gacgttctct gggacatctg gtccacctac ggaagaaagc ccctggtgct ctgccggctg
     1201 gacggcgggc cgtgggtaaa ccttcccgaa gaagggggca gacaggattt cgcactggaa
     1261 cgggtgaccc acctccgcat ctacagcccc ttcaccgggg agccccagaa acagtgatat
     1321 caaaaggctt cgctgatatg caaagggcgc gggaggaatc ccgcgccctt aatttttatt
     1381 ccaccgtgac gctctttgcg aggtttcgcg gctggtcgat gtgacagccc aggaacctgg
     1441 cgaagaagta ggaaaagagc tgcaggggag ccacggtgag gaacggggtg aactggtcca
     1501 gggtctcagg gacgaagaag atgtggtcag cagtcccgtc aagggaatcg tcaccctccg
     1561 tcgcgatggc gatgaccgga gacttcctcg ccttggcctc caggatgttg gagaaggtct
     1621 tttcgtgaag cccgtccttc ggggagatca ccaccaccgg gaccctggga tcgagaaggg
     1681 ctatggggcc gtgcttcatc tcccccgccg cgtaggcttc ggcgtggacg taggagattt
     1741 ctttcagctt cagggctccc tcaagggcca cagggtagga gatacccctt ccaaggaaga
     1801 ggaaatcccg ggcctcgttg tagagcatgg ccgtctcctg gaggctttcc tgcctctgga
     1861 gcacggattc aagcttgtag ggaagcgcgg tgagctcgtc gcagtatttt ctttcctgct
     1921 ccgggctcag cttgccctgc atcttggcaa ggtggatggc caggagatag agcacggcca
     1981 tctgccccat gaaggtcttg gtcgctgcga caccaatttc gggtcccgcc ttgagctgga
     2041 gcacgtcatg aacctctctg gcgatggtgg atcctatgac gttcgtgact gccaggcatc
     2101 gtgccccctt ctcccgggcg atccgctcag ccgcgagtgt gtccgccgtc tctcccgact
     2161 gggagacaaa aacggcaaga gtgtccggcc ccagggggat gttcctgtaa cggtattcgg
     2221 acgccacgtc cgccctgatg tcgaagtccg ttatggtttc gaggaaacgc tccgccacga
     2281 gggcggcgta ataggaggtt ccgcaggcaa tgatatggag cctcttccac gatgccacgt
     2341 cttcctttgt ccagggaagc tcggcggaaa ggtccaccgt cccctccgtc agcctttttg
     2401 ccatggagtt ccggagcacc gccccctgct cgttgatctc cttgagcatg aaatgggggt
     2461 agcctccctt gtcgaccatg gtgacgtccc agtcgatgtg ggtcgggatt ttttccagcg
     2521 tctcacccga tgcgttccag aagagaatgc ctccggagga aagctcggcg atgtcaccgt
     2581 cgtccaggta aaccacgtcc tgggtgtagg gaaggatggc cggcacgtcg gacgcacaga
     2641 gggtttcccc cttcggggag gctcccacca cgagggggga acctttcctg aggcagtaaa
     2701 tctttcccgg ggcttccctg gccatgatta cgagggcaaa agatccgctc agccgggcat
     2761 agagatccac aagggcctcc accatgctgc ccctggcgct gtagagcctc gacagaagct
     2821 cggccaccac ttcggtatcc gtctcggact tgtagtcgcc gcaggctcct tccgccagtt
     2881 ccctgcggat ttcccggtag ttttccacta tgccgttgtg gacgagcacg aggctcgagg
     2941 agaggtcgga atggggatgg gcgttcactt ccgtgactcc gccgtgggta gcccaccggg
     3001 tgtgcccgat gccctggctc cccgaaacgg ggtggtcctc caggattttc tcgagatctg
     3061 ccacctttcc cacgcccttg actatatgaa gctccggggc atccatgagt gcaacccccg
     3121 ctgaatcgta tcccctgtat tcaagactcc gcattccatt tatcagaacg tccttgacgt
     3181 tcctttcgcc gatatacccg actattccgc acatattcca aacaccgcct ttttccgctc
     3241 aaagaaaacc gccttcattt ttgtggcgga aattgcttcc cgcttttgta taaaggcttt
     3301 acgggcctgt tttcgcggcc ccggaggcat ccgccgaatc ttcgatactc ctccgtcctc
     3361 gtcagccggg agatctcccg gcgcaggcgc ttcaccctgg gc
//
LOCUS       DAOTHD010000114         3388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_33171_length_3388_cov_3.042304, whole genome shotgun sequence.
ACCESSION   DAOTHD010000114 DAOTHD010000000
VERSION     DAOTHD010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33171_length_3388_cov_3.042304"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="P5219_02935"
     CDS             <1..1032
                     /locus_tag="P5219_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRX25742.1"
                     /translation="DAFEKAFAEYVGTRHAVAIANGTAALHGAYFAAGVGPGDEVITS
                     PMTFAATSNAVLYLGGTVRFADMDPATLCLDPKKAGEGITTRTKVIAPVSYSGYPADL
                     AAFREAAEGSGAVLVEDACHALGAKRGKIPVGKEADLTAFSFHPVKHITTGEGGMVTT
                     DSDEYAERLRLFRTHGITKDPTRMEGEPDGSWSTEMQVLGFNYRLSDIHAALGLSQMK
                     KLDRFVARRKEIASLYDGLFSSVRGLDIPPGHEGHAYHLYPLRVPAERRKEFILKLKE
                     RGIGGMVHYPPVHLHPYYRKNFGWKKGDFPEAEAFYSREISLPMFYGLSNDDVMRVAE
                     EIRSIADAG"
     gene            1126..1569
                     /locus_tag="P5219_02940"
     CDS             1126..1569
                     /locus_tag="P5219_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRX25743.1"
                     /translation="MFSVKRLGVLLSVAVLFVIFTSVVAAADEKIGVIDSQKIIFQHP
                     RFEGVTKQLQQISRTKENEMKLAVDKEPDQNKKAQIFNTKRNELAQEEQRLMQPIFKE
                     AELAVRTVAKVKKVTVVIEKSAVYFGGIDITDDVIQELKKNAAKK"
     gene            1689..2522
                     /locus_tag="P5219_02945"
     CDS             1689..2522
                     /locus_tag="P5219_02945"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRX25744.1"
                     /translation="MAFKLLVTDIDETLSRGESVSPEVELACARLRENGWSLMVATGR
                     ILATAISHIRGIGSSLPAIVYDGGRVMDPSSGRSLCETPMDPGLALEIVEAGWNHPVE
                     LQIIGDERAFCRYSDVMTRDFFAASGVPVDDTMESPSSPSKVYRVIFHGVPGCIRTLQ
                     YELKSRFSERAEIVQAGDGFLDILPRGVSKGAALASWIDTLPERPELVVAAGDHHNDM
                     ELLSSAHIAAAPEDAADDVLKIARVIMPSADRHGFASLAEWLLEWEGSRYEIASPVVL
                     L"
     gene            2617..3114
                     /locus_tag="P5219_02950"
     CDS             2617..3114
                     /locus_tag="P5219_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HRX25745.1"
                     /translation="MPEITRWSLVQLEFPEDCNIILGQSHFIKTVEDLFEVLVTSSPS
                     LRFGIAFCEASGDCLIRRDGNDEELVAVATANAEKIACGHSFVVVLRNGYPINVLNRI
                     KDCQEVCRIFAATANPIQAIVCESGQGRGIAGVIDGFVPRGVETGEDVEKRKNLLRSI
                     IGYKR"
     gene            3139..>3388
                     /locus_tag="P5219_02955"
     CDS             3139..>3388
                     /locus_tag="P5219_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25746.1"
                     /translation="MDRKKIILLLLLPFFFCPRPGFAEDLAIGVEDGWIWQVAVLPPP
                     GGWETESGISALGAVRYAEWKVKDSPDGVAGRDIRFLKE"
BASE COUNT          743 a          904 c         1026 g          715 t
ORIGIN      
        1 gacgcttttg agaaagcctt cgcggagtac gttggaaccc gacatgcggt ggccatcgcc
       61 aacggaacgg ccgccctcca cggggcctat ttcgcggcgg gcgtgggacc cggagacgaa
      121 gtgatcacgt ctcccatgac ttttgcggcc acgagcaacg ccgtcctcta tctcggggga
      181 accgtgcgct ttgcggacat ggatccggcc accctctgcc tcgatccgaa aaaagcagga
      241 gagggaataa ccacgaggac aaaagtgatc gccccggtga gctactcggg atacccggcg
      301 gacctggccg ccttccggga agcggcggaa ggctccggcg cggtcctggt cgaggatgcc
      361 tgccatgccc tcggcgcgaa gaggggaaag atccctgtgg gaaaagaagc cgacctgacc
      421 gctttcagct tccatccggt aaagcacatc accaccggcg aaggaggaat ggtcactacc
      481 gactcggatg aatatgccga acggctgcgg cttttccgga cccacggcat cacgaaggat
      541 ccgacaagaa tggaagggga gcccgatgga tcatggtcaa cggagatgca ggtcctcggc
      601 ttcaactaca ggctgagcga catccacgcc gcccttgggc tgagccagat gaagaagctc
      661 gaccggttcg ttgcccgaag aaaggagatc gcctctctgt atgacggtct tttctcttct
      721 gtgcggggcc ttgatattcc tccaggccac gaggggcacg cctatcatct ctatccgctc
      781 agggttcctg cggaacggcg gaaggaattt atcctgaaac tgaaggagag gggaatcgga
      841 ggaatggttc attacccgcc ggtgcacctc cacccctact acaggaagaa tttcggctgg
      901 aagaaggggg attttcccga ggctgaggcc ttttattcca gggagataag ccttcccatg
      961 ttctacggtt tgtcaaacga cgacgtcatg agggtggcgg aggaaatccg gagcatcgcc
     1021 gatgccgggt gatttacccg taaatttacc tggactttcg tagggtttcc agctacaatt
     1081 tcaaccgtgt ccgatatttc tgagacgtat cgagggggta gttgtatgtt ttctgtaaag
     1141 cgtttgggcg ttcttctttc cgtggcggtg ctgtttgtca tctttacttc cgtcgtggcc
     1201 gctgccgatg aaaagatcgg cgtgatcgat tcccagaaga tcatcttcca gcacccgagg
     1261 ttcgagggcg tgacaaagca gcttcagcag atcagcagga ccaaggaaaa cgagatgaag
     1321 ctggctgttg acaaggaacc tgatcagaac aagaaggccc agatcttcaa cacgaaacgg
     1381 aacgagcttg cccaggaaga gcagcggctc atgcagccca ttttcaagga ggccgagctt
     1441 gcggtccgca ccgtggcgaa ggtcaagaaa gtgacagtgg tcatcgagaa gtcggcggtc
     1501 tacttcggcg gcatcgacat taccgatgac gtaatccagg agctcaagaa aaacgctgcg
     1561 aagaaataga gtccagcctc acaccgctgc ggcggtcatg aggttatgag agcgacggag
     1621 gcccgcctcc cccctgagaa cgcggggggt tgtccaggcc tccttttttt ctattggaga
     1681 gtgagattat ggcattcaag cttcttgtca cagatattga cgaaactctt tcccggggcg
     1741 agtccgtttc cccggaggtg gaacttgcct gcgcccgcct gagggagaac ggctggagcc
     1801 tcatggtggc aacgggacga attctcgcaa cggccatatc ccatatcagg gggataggtt
     1861 cgtcccttcc tgctatagtg tatgacgggg gcagggtgat ggatccatcc agcggcaggt
     1921 ctctctgcga aactcccatg gaccccgggc ttgcgcttga gatcgttgag gcagggtgga
     1981 atcatccggt tgaactccag atcatcgggg acgaaagagc attttgccgg tacagcgatg
     2041 tgatgacccg ggattttttt gccgcttccg gcgtgccggt ggatgacacc atggaaagcc
     2101 catcttcccc ctcgaaagtg tacagggtga ttttccatgg tgttcccggc tgcatacgaa
     2161 cactccagta cgaactgaaa agccggtttt ccgaaagggc ggaaattgtc caggcgggag
     2221 acggctttct cgatattctt cccaggggag tgtccaaggg agcggccctg gcttcatgga
     2281 tcgatacgct cccggagcgg ccggagctgg tggtggctgc gggagatcat cacaacgaca
     2341 tggagcttct ctcttcggcc catattgccg cagcccctga agatgccgcc gacgatgtgc
     2401 tgaaaatagc ccgtgtcatc atgccgtcgg cggacaggca cggattcgcc tccctggcgg
     2461 aatggctcct ggagtgggag gggagccgtt acgaaatcgc ctcccctgtt gtattattat
     2521 aaagactcct ttccctttgt aatgaaaaga aaacacgctt ttgtcaaaag caggtttcgg
     2581 aatgcagcga acgactaaac gaggagggaa acaaccgtgc cggaaatcac caggtggtcc
     2641 cttgtgcagc tggagttccc cgaggactgc aatatcatcc ttgggcagag ccactttatc
     2701 aagaccgtgg aggatctttt cgaagtcctc gtaacctcgt cgccgtctct ccgttttgga
     2761 atcgccttct gcgaagcttc cggagactgt ctcatccgga gggacggaaa cgatgaagaa
     2821 ctggtggcgg tggccacggc gaacgccgaa aaaatcgcct gcggacattc tttcgtggtg
     2881 gttctccgga acgggtatcc catcaacgtt ctcaacagga tcaaggactg tcaggaagtc
     2941 tgccgtattt tcgccgccac ggcgaaccct attcaggcca tcgtgtgcga gagcggccag
     3001 ggacgaggga ttgccggggt tatcgacggc tttgtcccca ggggtgtgga gaccggtgag
     3061 gatgtggaga aaagaaagaa tctgctccgc tcgatcatcg gctacaaaag gtgattcttt
     3121 cctcccccgg cctgacagat ggatcgtaaa aaaattattc ttctccttct cctgcccttc
     3181 tttttttgcc ctcgaccggg ttttgccgag gaccttgcca taggggtaga ggacggatgg
     3241 atctggcagg tggcggtgct gccccctccg ggcggctggg agaccgagag cgggatatcc
     3301 gctctcgggg ccgtccggta tgccgaatgg aaggtgaagg acagccccga cggagtggcc
     3361 gggagagaca tccgtttttt gaaggaac
//
LOCUS       DAOTHD010000115         3378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_33282_length_3378_cov_3.116160, whole genome shotgun sequence.
ACCESSION   DAOTHD010000115 DAOTHD010000000
VERSION     DAOTHD010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33282_length_3378_cov_3.116160"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1640)
                     /locus_tag="P5219_02960"
     CDS             complement(<1..1640)
                     /locus_tag="P5219_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HRX25747.1"
                     /translation="MNAHIAQRVSQELKISPSSVEAVLSLLEQDATVPFIARYRKEAT
                     GSLDEEVIFAIKERYARIEELEARKKAVLSSLEERNLLSAELGEKVRNAPSLTVLEDI
                     YLPFRPKRRTRASVAEEKGLAPLAGQLLLQKGSDPAVLALPFVSEEKGVPSGEEALQG
                     ALDIIAERISEDGEARRKMRLLFGKRGKIRSSVAKGKEEEGANFRDYFAWEEPAAPAP
                     SHRVLAMFRGEKEKILSLSVLPPEDEALRALREIFLHGSGPDSILVDRAIVDGYRRLL
                     APSMETELRNALKKKADREAISVFAGNVREVLLAPPLGPKNILAVDPGFRTGCKFVCL
                     NRQGDLLHHGVIYPHPPRGDKKGSAEIIRRAAEEYGIEAIAVGNGTAGRETERFLREL
                     GLPSSIIITMVNESGASVYSASETARREFPDHDLTVRGAVSIGRRLLDPLAELVKIDP
                     KSIGVGQYQHDVDQKELKKALDTVVEFCVHAVGVDLNTASLELLSSVSGLGQSTAGRI
                     IRYREANGPFPTRKELLNVPRLGPKTFEQAAGFLRVSGG"
     gene            1734..2354
                     /locus_tag="P5219_02965"
     CDS             1734..2354
                     /locus_tag="P5219_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRX25748.1"
                     /translation="MEKNWWMPLYGILFGIYVPEEVRELKGDVVMHIADTPSTVYGAI
                     RRLVKVLSPRWIVHTGDLADDVKLELSPAELPRYRSKLENLRRALEGDDSPGLVIVTG
                     NHDNERAVREMFPKSIVFRERGRIELCGEGFNLSHDRAGLGDPPLKYNLFGHEPSRND
                     FSEQGRFFLNGLLGVHIISPSLEKVYCLPYPGYVDDGRLGRRKRGL"
     gene            2373..>3378
                     /locus_tag="P5219_02970"
     CDS             2373..>3378
                     /locus_tag="P5219_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25749.1"
                     /translation="MLSIVRGGPRLLLLSGNEDAMKSLVSSRFTCTEHTLEGAFDSAG
                     EGQSVLLFRGGQWGSGRRFLMAAAAADEILALFLNSGAEARPVSVRIMPRLLFFRVFG
                     EKERVIGQIARDYSAERGRLRSVLRVPKKGSIAVCFTAKPLNRPLSLADMMDEVLHVE
                     GVSFETLLNSLRSRALWYFSEGLENRQWNELEIRIYDSWGRYLDHYERLRIVLESLEA
                     GMILGEGWGKDFAHILMAVKIYRLRLFTFLPPSTIKEILIGLEYGADGSRLVDFDLYS
                     GKEKISWGGLGGKGKERGERGSMGTGLRAKLFSQLFPEDRQHLQEWESRILRQRTGEG
                     N"
BASE COUNT          673 a          903 c         1036 g          766 t
ORIGIN      
        1 ccaccggaga ccctgaggaa tccggcggcc tgttcgaagg tcttcggccc gagccgaggg
       61 acgttcagaa gctctttcct tgtggggaag ggaccgttgg cctccctgta cctgattatc
      121 cgcccggcag tggactgccc gagaccggaa acagaggaga ggagctcaag gctcgctgtg
      181 ttcaggtcga cgcccacggc gtggacacaa aactccacca cggtgtcgag agccttcttc
      241 agttccttct ggtccacatc gtgctggtac tggcccacgc cgattgactt gggatcgatc
      301 ttcaccagtt ccgcaagggg gtcgagaagt ctccggccga tggatacggc tccccggacg
      361 gtgaggtcgt gatcggggaa ctctcttctg gcggtctccg aagcagaata gaccgaagcc
      421 ccgctttcgt tcaccatggt tatgatgatg gatgaaggga ggccgagttc cctgagaaat
      481 cgctctgttt cccttccggc ggttccgttg cccacggcga tcgcttcgat gccgtattct
      541 tccgcggcac ggcggataat ttccgcgctc ccttttttat ctccccttgg agggtgggga
      601 tagatcactc cgtggtggag aaggtctccc tgccggttca ggcagacgaa cttacaccct
      661 gtcctgaatc cggggtctac ggcgaggatg ttcttcggcc cgaggggtgg ggccaggagc
      721 acttctctta cgtttcccgc aaaaacggaa atggcctcac ggtcagcttt tttcttcagg
      781 gcgttcctca gttccgtttc catggacgga gcgagaagtc gacggtatcc gtcaacgatc
      841 gcccggtcaa caaggatgga atcgggaccg ctgccgtgga gaaaaatttc cctgagtgcc
      901 ctcagcgcct cgtcctcggg ggggaggaca gagaggctga gtattttttc cttttccccc
      961 cggaacatgg ccaggaccct gtgggaggga gccggtgcag ccggttcttc ccaggcgaag
     1021 taatccctga agttggctcc ttcctcttcc tttcctttgg ctactgaact cctgattttt
     1081 ccacgttttc cgaaaagaag cctcatcttc ctccgggcct caccgtcttc gcttatccgt
     1141 tcggcgatga tgtcgagggc cccctgaagg gcctcctctc cggaaggcac gcccttttct
     1201 tcagaaacga atgggagggc gagaacagcc gggtcagacc ccttctggag cagcagctgc
     1261 ccggcgaggg gagccagtcc cttttcttcc gcgacggatg ccctggttct tctcttgggc
     1321 ctgaagggaa ggtatatgtc ctcgaggacg gtcagtgacg gggcgttccg gaccttttcc
     1381 ccgagttcgg ccgaaagaag gtttctttcc tcgagggatg aaagcaccgc cttttttctg
     1441 gcttcaagct cttcgatcct ggcataacgc tcctttatgg cgaaaatgac ctcttcatca
     1501 agggagccgg tggcttcctt cctgtaccgt gcaataaagg gaacggtagc gtcctgttcg
     1561 agaagggaca gaacggcctc cacggacgaa ggggatattt tgagctcctg ggaaacgcgc
     1621 tgggcgatgt gggcgttcat agaaagcctc cggttctttt ttcctcattc tagcacggca
     1681 cgaaaagccg ggccatatca tggtagaatc tggctggaag agaggagccg tccatggaga
     1741 aaaattggtg gatgccgttg tatggcattc ttttcggaat atacgtcccc gaggaggtca
     1801 gggagctgaa aggcgatgtg gtcatgcata tcgccgacac cccctcgacc gtttacgggg
     1861 cgattcgcag gcttgtgaag gtgttgtctc cccgctggat tgtccatacg ggcgacttgg
     1921 ccgacgatgt gaagcttgag ctttctcccg ccgaactgcc gagatacaga tccaagctgg
     1981 agaatctcag gcgtgccctg gaaggagatg attcaccggg cctggtcatc gtaaccggaa
     2041 atcatgacaa cgaaagggct gtcagggaaa tgttcccgaa aagcatagtt ttcagggaac
     2101 gggggcggat cgaactctgc ggcgaaggat tcaacctcag ccatgacagg gccggccttg
     2161 gagacccgcc tctcaagtac aatcttttcg gtcatgaacc gtcgcggaat gacttttcag
     2221 agcaggggcg cttttttttg aacgggctcc tgggcgttca tattatttct ccttccctgg
     2281 agaaagtgta ctgcctgccg tatccgggat atgtggacga cgggcggctg gggcggagaa
     2341 aaaggggatt gtgacgaaaa ggggggagtc tcgtgctttc catcgtccgg ggaggacccc
     2401 gtcttctgtt gctgagcggg aacgaggacg caatgaagtc tctggtttct tcgcggttca
     2461 cgtgtacaga acataccctg gaaggtgctt ttgacagcgc cggagagggg cagtccgttc
     2521 ttcttttccg gggaggtcaa tgggggagcg gacgacgttt tctcatggcc gcagccgcgg
     2581 ccgatgaaat ccttgccctt tttctgaaca gcggggcgga ggcccggccc gtgtcggtaa
     2641 gaatcatgcc ccggcttctt tttttccggg tctttggaga aaaagaacgg gtcatcggcc
     2701 agatagcccg ggactactct gcggaacggg gaagactccg gtccgtcctc cgggttccga
     2761 agaaaggcag cattgcggtc tgttttacgg cgaagcccct gaacaggccg ctttctctcg
     2821 ctgatatgat ggacgaagtt ctccacgtgg aaggcgtgtc ctttgaaaca cttctcaact
     2881 cccttcggag cagggccctg tggtatttca gcgaggggct ggagaaccgg cagtggaacg
     2941 aactggaaat acggatttat gattcgtggg gcaggtatct cgaccattat gaacggctcc
     3001 gcatcgtcct ggaaagcctc gaggcaggca tgatcctggg ggaaggatgg gggaaggatt
     3061 ttgcccacat ccttatggcg gtgaaaattt accgcctgag gctttttacc tttctccccc
     3121 catcgacgat aaaggaaatc ctcataggcc tggaatacgg agcggacggt tcccggcttg
     3181 ttgacttcga cctgtacagc ggcaaggaga agatctcctg ggggggattg ggcggaaagg
     3241 ggaaggagcg gggagagcgg ggctccatgg gaaccggtct cagggcgaaa cttttttccc
     3301 agctttttcc ggaggacagg cagcaccttc aagagtggga aagccgcatc ctccggcagc
     3361 gtacgggaga aggaaacc
//
LOCUS       DAOTHD010000116         3372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_33362_length_3372_cov_2.838408, whole genome shotgun sequence.
ACCESSION   DAOTHD010000116 DAOTHD010000000
VERSION     DAOTHD010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33362_length_3372_cov_2.838408"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="P5219_02975"
     CDS             complement(<1..379)
                     /locus_tag="P5219_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRX25750.1"
                     /translation="MKEQVWKLVDEVKEFVVEARHRIHENPELGFKEFETTAFVKKEL
                     ESAGIELVPLDLETGVLAVIRGTAEWKGQGEPPVAGLRADMDALPIEEATGVPYSSKN
                     KGVMHACGHDGHTAILLGTAKALQ"
     gene            complement(523..1455)
                     /locus_tag="P5219_02980"
     CDS             complement(523..1455)
                     /locus_tag="P5219_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRX25751.1"
                     /translation="MAFLSLPGAVEIMEVCPRDGFQSVKEFIPTEHKVAIIDGLAGTG
                     ISAMEVTSFVSPKAIPQMADAADVMAEFNTKWKGKVTSIALIPNLRGAEGALASGVDW
                     MNFVISASGDHNMANTRRTVDESIAEMEKVAALKGSTKLRVSVATAFACPFAGPVAPE
                     KVLRVIDRALEAGADGITMADTIGTADPKYVTSTLSVIREKYGDYPFTLHLHDTYGMA
                     LANMAAAMGLGFASFDAAAGGLGGCPFAPGAAGNAATEDMVNMAESMGIKTGIDILKV
                     LSVVRTMESLYGLKINSHLAATKMGRESGEGCCS"
     gene            complement(1457..2653)
                     /locus_tag="P5219_02985"
     CDS             complement(1457..2653)
                     /locus_tag="P5219_02985"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRX25752.1"
                     /translation="MTRKGALDGLRVLDLTRVLAGPFCTMMFADMGAEIIKIEQAGTG
                     DDTRQMGPFKNGESAYFMNLNRNKKGVTLNLKNPKGKAIFLDLVKQADVVIENFRPGT
                     MEKLGLGYEELKKVNPAIVYAAISGFGHTGRYSQRPGYDIISQAMSGLMSTTGWPGGE
                     PTRTGTAMGDVLGGLSCAIGILAAVFNKIQTGEGQKVDVALVDSAVASLEIINMIYLV
                     EGRIPQRIGNRYESTYPYDSFRGSDGSLVIGAGNDKLWVSLCKVMGKPELAEDPRYLH
                     VPDRVARHEELKAVVEEWSTKLTVDEIYEKVNNAGIPCAPIYNIEQVVNDPHIAGDRE
                     MFVEMDHPTAGKLKVTGSHIKLSGTPSGVRTPSPTLGQHNGDVLGELLGLTAEDLKAL
                     SEEGAI"
     gene            complement(2653..>3372)
                     /locus_tag="P5219_02990"
     CDS             complement(2653..>3372)
                     /locus_tag="P5219_02990"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRX25753.1"
                     /translation="KTLAEEVASYVRKELLLSDTYPPGSFIREEELATKLGISRAPVR
                     EGLKMLEGLGLLRSIPQKGSLVVAFSPGEIEELYDIRYALEEILFREIIRRGTFTGEQ
                     QGALKKILSRMGLLGSSGDSREEILLEFSRLDMEFHLSLAALAGREWTLRLLRTVYHQ
                     IQHALLRDLATESDMDALVAEHKEILDGLQRGDLEELRKGRFFSYFERRLDPNKGKVR
                     PELSETPGEDNQTGQKKGEKA"
BASE COUNT          546 a         1027 c         1046 g          753 t
ORIGIN      
        1 tctggagcgc ttttgccgta cccagcagga tggccgtgtg gccgtcatgg ccgcaggcgt
       61 gcatgactcc cttgtttttc gaggagtagg gaactcctgt ggcctcttcg atgggaaggg
      121 cgtccatatc ggcccgaaga cctgccacgg ggggttcccc ctgtcccttc cattcagccg
      181 ttccccggat gacggccaga acgccggtct ccagatccag gggcaccagt tcgattcccg
      241 ccgactcaag ttcttttttg acgaacgccg tggtctcgaa ttccttgaat ccgagctccg
      301 ggttttcatg aatgcggtgc cgtgcctcga ccacaaactc cttcacttcg tccacgagct
      361 tccagacctg ttccttcatt tcccgacctc ccttttgaaa aaaaccgtgc tccttctgca
      421 gaaggggcac ggtgtatttc gctcaagata atataataca cagcctgcat aatgcaaccc
      481 caagccgaag atttccggac tcttcggcta attgtcagct tctcaggaac agcatccctc
      541 gccgctctcc cggcccatct tcgttgccgc caggtggctg ttgatcttca gcccgtagag
      601 ggactccatg gtccgcacca cggaaaggac cttcaggata tcgatgcccg ttttgatccc
      661 catgctctcg gccatgttca ccatgtcctc cgtggcggcg ttccccgccg cgcccggcgc
      721 gaatgggcat ccccccagtc cgccggcggc cgcgtcgaag gaggcgaagc ccagtcccat
      781 ggcggcggcc atgttggcca gggccatgcc gtaggtgtcg tgaagatgga gtgtgaaggg
      841 ataatcgccg tatttctccc ggatgaccga cagggtggag gtcacgtact tcgggtccgc
      901 tgtgccgatg gtgtcggcca tggtgattcc gtccgccccg gcctcgaggg ccctgtcgat
      961 gaccctgagc actttttccg gcgccacggg accggcgaag gggcaggcga aggcggtggc
     1021 cacggagacc cggagctttg tggatccctt cagggcggcg accttctcca tctccgcaat
     1081 ggattcatcc accgttctcc tggtattggc catgttgtgg tccccgctgg cggaaatgac
     1141 gaagttcatc cagtcaacgc cggaggccag ggcgccctcg gctccccgga ggttgggaat
     1201 gagggcgatg gacgtgacct ttcccttcca ttttgtattg aattccgcca ttacgtcggc
     1261 ggcgtcggcc atctggggaa tggccttcgg gctgacgaag gaggtcactt ccatggcgga
     1321 aatgcctgtt ccggccaggc cgtcgatgat ggccaccttg tgctccgtgg ggatgaattc
     1381 tttcacgctc tggaagccgt ccctggggca gacctccatg atttcgactg cgccggggag
     1441 ggacaggaag gccatgtcag atcgctccct cttcgctgag ggccttcagg tcctcagcgg
     1501 taagaccgag gagttcgccg agcacgtccc cgttgtgctg acccagggtc ggggaggggg
     1561 tgcgcacgcc cgacggagtg ccggagagct tgatgtggct tcccgtcacc ttgagtttgc
     1621 ccgccgtggg atggtccatc tccacgaaca tctcccggtc tccggcgatg tgggggtcat
     1681 tgaccacctg ctcgatgttg tagatggggg cgcagggaat gcccgcgttg ttgaccttct
     1741 cgtagatttc gtccacggtg agcttcgtgg accattcctc cacgacggct ttcagctcct
     1801 cgtgcctggc cacgcggtcg ggaacatgaa ggtaccgtgg gtcttcggcg agttccggct
     1861 ttcccatgac cttgcagagg ctgacccaga gcttgtcgtt gccggcgccg atgaccaggc
     1921 tgccgtcgga gccccggaag gagtcgtagg ggtaggtgga ttcgtaccgg ttgccgatgc
     1981 gctggggaat ccggccttcc acaaggtaga tcatgttgat gatctcgagg ctggccactg
     2041 ccgagtccac cagggccaca tcaactttct ggccctcgcc ggtctggatt ttgttgaata
     2101 ccgccgccag gatgccgatg gcgcaggaga ggccgccgag gacgtccccc atggcggtgc
     2161 cggtgcgggt gggctcccct cccggccagc cggtggtgct catgaggccg ctcatggcct
     2221 gggagatgat gtcatagccg gggcgctggc tgtaccgtcc agtgtggccg aaaccggaaa
     2281 tggcggcgta gacgatggcc gggttgacct tcttcagttc ctcgtagccc aggccgagct
     2341 tttccatggt gccgggccgg aagttctcga tcactacgtc cgcctgtttc accaggtcga
     2401 ggaagatggc tttccccttc gggttcttca ggttcagggt gacgcccttc ttgttcctgt
     2461 tgaggttcat gaagtaggcg ctttctccgt tcttgaacgg ccccatctgg cgggtgtcgt
     2521 caccggtgcc tgcctgttcg atcttgatga tctccgcccc catgtcggcg aacatcatgg
     2581 tgcagaacgg cccggcaagc acccgggtga ggtccaggac gcgaagtcca tcgagggctc
     2641 cttttcgtgt catcatgctt tttccccttt cttctgtccg gtttgattgt cttcgcccgg
     2701 ggtttccgaa agctccgggc gcacttttcc cttgttcgga tccagcctgc gctcgaaata
     2761 gctgaagaac cgtcctttcc tgagttcctc aagatctccc cgctgcaggc cgtcgaggat
     2821 ctccttgtgt tccgccacca gggcatccat gtccgactcg gtggcgaggt cgcggagaag
     2881 ggcgtgctgg atctggtggt acaccgttct gaggagccgc agggtccatt ccctgccggc
     2941 gagggccgcc agggagaggt ggaattccat gtccagcctg ctgaattcga ggagaatttc
     3001 ctccctggag tcaccggaag agccgaggag ccccatccgg gagaggattt tcttcagggc
     3061 tccctgctgc tctccggtga atgtgcccct gcggatgatc tcccggaaga gaatctcctc
     3121 gagggcatag cggatgtcgt agagttcttc gatttccccc ggggaaaagg ccaccacgag
     3181 ggagcccttc tgggggatgg agcggaggag tcccagcccc tccagcatct tgagcccttc
     3241 cctgacaggg gcacggctga tgccgagctt cgtggcgagt tcctcctccc ggataaagga
     3301 accggggggg tacgtatcgg agaggagcag ctccttccgg acgtaggagg ccacttcttc
     3361 ggcgagggtt tt
//
LOCUS       DAOTHD010000117         3365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_33450_length_3365_cov_3.630514, whole genome shotgun sequence.
ACCESSION   DAOTHD010000117 DAOTHD010000000
VERSION     DAOTHD010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33450_length_3365_cov_3.630514"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1671
                     /gene="ptsP"
                     /locus_tag="P5219_02995"
     CDS             <1..1671
                     /gene="ptsP"
                     /locus_tag="P5219_02995"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HRX25754.1"
                     /translation="HWKEQIDILREFVEDPEQELERFRTALEFAGEQIRESYSRVLET
                     VGPGEAAIFRSHGMMLKDPDFVGQIERMILDGSVNAEWAVKQVADSLIQIFESMGNDY
                     MKARSDDVKDISDRVIKLLLQVGGIDLSNLREPSIIVARELTPSDISQMDRKMTLGMA
                     SETGGKTSHTAILARTLEIPAVMGVKELLDEVENGDLMIIDGEAGQVYVNPDGETVER
                     YRERLERYECFKKSLSEVRGLKTVTLDGVEIELEANVASPKDLDDVFKNDGEGVGLYR
                     TEFLYFDSSLLPPEDTQFEQYRDVVEAMKGRPVVIRTLDIGGDKKVDYLSIPEEQNPF
                     LGYRAIRLCLDRQEIFKVQLRAILRASAYGRVRILFPMISSLEELRSAKNILEETKED
                     LRSQDVPFDESVQSGIMVEVPSAAILSDHFARETDFFSIGTNDLIQYAVAVDRGNEKL
                     SHLYSQYHPAVLRLIKTVLDNGTAAGIRVGMCGESAGDPKLIPILLGMGLTEFSMNPS
                     SILRARWILRNLRKSDLEEAASTALSLATAEEVEEYCSGLLQSLNLCR"
     gene            1713..2036
                     /locus_tag="P5219_03000"
     CDS             1713..2036
                     /locus_tag="P5219_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25755.1"
                     /translation="MNDAARTDRKSQSLPEMLRPLFWDAEWSSVDFDRNRTAVIERIL
                     NLGNEEQLAWLRRNVSLDEIRAVVSSSRRLSRKTARCWQNFFGLREEDMRCFGMFSTN
                     PDNLF"
     gene            1994..2632
                     /locus_tag="P5219_03005"
     CDS             1994..2632
                     /locus_tag="P5219_03005"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRX25756.1"
                     /translation="MFWDVLDKPRQSLLKSIVASPPIRGAYLAGGTALALQYGHRLSE
                     DFDWFLPESFDPGKLPALLECFGTVSVAETHAGTFHGWIDGIRVTWLHYPNPLLDPLQ
                     EVRDLPGLLLSSPRDTGLMKWAALSDRGARKDFLDLYELGLQGITLEELFLLLPRKFP
                     GRRLNLYHMIKGLSYFDDADNDPWPVMLRDVSWSALQRYFLSSQTRLLRSLP"
     gene            2779..>3365
                     /locus_tag="P5219_03010"
     CDS             2779..>3365
                     /locus_tag="P5219_03010"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRX25757.1"
                     /translation="MEYVSVSRLQSGMVLSGDLIAPNGRFILPKGARLTPKNIMSLKI
                     WGVSEVPVAKAKDLEESSGEELLYSSSVMEEARNMVLDLFSGTPPASPIMEEVFTVAV
                     RVNAARIAEGLPARRPETFSLSLPVKPSISDEKIDMEDILRTEVGLTSFPDVYFRVSE
                     ALQSPKSSVSHIADLISRDTALSATLLKLANSAIYG"
BASE COUNT          715 a         1065 c          961 g          624 t
ORIGIN      
        1 cactggaaag aacagatcga catcctccgg gagttcgttg aagatccgga acaggagctc
       61 gaacgctttc gcaccgccct cgagtttgcc ggagagcaga tccgcgagtc ctattccagg
      121 gtgctcgaaa ccgtcggacc gggagaagcg gccatcttca ggtcccacgg catgatgctg
      181 aaggatcctg atttcgtcgg ccagatcgag cgcatgatcc tcgacgggag cgtcaacgcc
      241 gaatgggcgg tgaagcaggt ggctgacagc cttatccaga tcttcgaaag catgggaaac
      301 gattatatga aggcccggtc cgacgatgtg aaggacatct ccgaccgggt gatcaagctg
      361 ctcctccagg tcggcgggat cgacctctcg aacctcaggg agccgtccat catcgtggcg
      421 agggagctta ctccctcgga catctcccag atggacagga agatgaccct gggcatggcc
      481 tccgaaacgg gaggcaagac atcccacacc gccatactcg cccggactct ggaaatcccg
      541 gccgtcatgg gagtgaaaga gctcctcgac gaagtggaaa acggagatct catgatcatc
      601 gacggcgaag cgggccaggt gtatgtgaac cccgacgggg agacggtgga acgataccgg
      661 gagcggctgg agcggtacga gtgcttcaag aaaagcctct cggaagtccg gggattgaag
      721 acggtcaccc ttgacggggt ggagatcgaa ctcgaggcca acgtggcctc cccgaaggac
      781 ctggacgacg tcttcaagaa cgacggcgag ggcgtggggc tctaccggac ggaatttctc
      841 tatttcgaca gctccctcct cccccccgag gacacccagt tcgagcagta cagggacgtg
      901 gtggaggcca tgaagggacg gccggtggtc atcaggaccc ttgacatcgg gggagacaag
      961 aaggtggact acctctccat ccccgaagag cagaacccct tcctgggcta ccgggccatc
     1021 aggctctgcc tggaccggca ggagatcttc aaggtccagc tccgggccat cctccgggcc
     1081 agcgcctacg gccgggtccg catcctcttt cccatgatct cgagcctcga ggagctccgt
     1141 tcggcaaaaa acatccttga ggagaccaag gaagacctcc ggtcccagga cgtgcccttc
     1201 gacgaatccg tccagtcggg catcatggtg gaggtgccgt cggcggccat cctctccgac
     1261 cacttcgcca gggaaacgga ctttttcagc atcggcacca acgacctgat ccagtacgcc
     1321 gtggcggttg accggggaaa cgaaaaactt tcccacctct actcccagta ccacccggcg
     1381 gtgctccggc tcataaaaac ggtcctcgac aacggaaccg cggcgggcat ccgggtcggc
     1441 atgtgcggcg aatcagccgg agacccgaag ctcattccca tcctcctcgg catggggctc
     1501 accgaattca gcatgaaccc ttcgtccatc ctccgggccc ggtggatcct ccggaacctc
     1561 cggaaaagcg acctggagga ggcggcatcc acagccctgt cgctggccac ggcggaagag
     1621 gtggaggaat actgctccgg tctgctccag agcctgaacc tctgccgctg agggaaaaga
     1681 aatttccctc agggaaggat tataattagg ccatgaacga tgccgcccgc accgaccgga
     1741 aatcccagtc cctgcccgaa atgctgcgcc ctctcttctg ggacgcggaa tggtcctccg
     1801 tggatttcga caggaacagg acggcggtga tcgagcgtat tctgaacctg gggaacgagg
     1861 agcagcttgc ctggctccgc cggaacgtct cgctggacga aatccgtgca gtggtttcat
     1921 cgagcaggcg gctgagcaga aagacggccc ggtgctggca gaactttttc gggctcaggg
     1981 aggaggacat gcggtgtttt gggatgttct cgacaaaccc agacaatctc ttctgaaaag
     2041 catcgtcgcc tcccctccca tccggggggc ctacctcgcg gggggcactg ccctggccct
     2101 gcagtacggc cacaggcttt ccgaagattt cgactggttc ctgcccgaaa gcttcgaccc
     2161 cgggaaactg cccgccctgc ttgaatgctt cggaaccgtc tcggtcgccg agacccacgc
     2221 cggcaccttc cacgggtgga tcgacggcat acgggtaaca tggctccatt atcccaaccc
     2281 ccttctcgat cccctccagg aggttcggga tcttcccggg ctgctgcttt cctcaccccg
     2341 ggacacggga ctcatgaaat gggccgccct ctccgaccgg ggggcccgga aggactttct
     2401 cgacctctat gaactgggcc tccaggggat caccctcgaa gagctcttcc tccttctgcc
     2461 caggaaattt cccgggcggc gcctcaacct ttaccacatg atcaagggac tgagctactt
     2521 cgacgacgcc gacaacgacc cctggcccgt catgctcagg gacgtgagct ggagcgccct
     2581 ccagcggtat ttcctctcat cccagaccag gctgctccgg tccctcccct gaaatgcctt
     2641 tttctgaacg aggaggagac aacatttccc ctccttttca gaagtgtaca cttgacaatt
     2701 tgtgaacaat atggttaaaa gataaaaggt gtattttatt ctcgctccgc cgctgcggag
     2761 cagaatccgg gggacagcat ggaatacgta tccgtcagcc gtcttcaatc gggcatggtg
     2821 ctttccgggg atcttatcgc cccgaacggc cggttcatat tgcccaaggg agcccgactg
     2881 acccccaaaa acatcatgag cctcaagatc tggggagtct cagaagttcc cgtggccaaa
     2941 gcaaaagacc tggaagaatc gtccggagag gaacttcttt attcctcctc cgtcatggaa
     3001 gaagccagga acatggtgct tgacctgttc tcgggcacac ctcccgcctc gcccataatg
     3061 gaagaggttt tcaccgtcgc cgtgagggtg aacgccgcac ggatcgccga aggacttccg
     3121 gcgcggcgcc ccgaaacctt ctccctgtcc cttcccgtca agcccagcat ctccgacgag
     3181 aaaatcgaca tggaggacat tctcaggacc gaagtgggac tcacctcctt tccggacgtg
     3241 tatttccgcg tttcggaggc cctgcagtcc cccaagagct ccgtctccca catcgccgac
     3301 ctcatcagca gggacaccgc cctctcggcc acccttctca agctggccaa cagcgccatt
     3361 tacgg
//
LOCUS       DAOTHD010000118         3347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_33684_length_3347_cov_3.328676, whole genome shotgun sequence.
ACCESSION   DAOTHD010000118 DAOTHD010000000
VERSION     DAOTHD010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33684_length_3347_cov_3.328676"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..717)
                     /locus_tag="P5219_03015"
     CDS             complement(196..717)
                     /locus_tag="P5219_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HRX25758.1"
                     /translation="MNGRLGIISDTHGHEEAWKKALSRWGSVDGVLHCGDVLADIATG
                     LEEMIRGAPFPVIISRGNCDRPEDEALLGWPVMAPYATVWWHSRLILVGHGAPFQPLR
                     ELGLRCRADLVIYGHTHVGSIVREEGTIFLNPGSAALPKGRDPASAAVLDGDGIRIFV
                     LENGETLHQEPWI"
     gene            862..1227
                     /locus_tag="P5219_03020"
     CDS             862..1227
                     /locus_tag="P5219_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25759.1"
                     /translation="MNEHDIFEEMKKLLDSEGLVTARLVESVTKTSQLTASTVPEIQD
                     LFRQWLSLVAREVKRMAEPGKDLPLAGTAGMIGISPSSLLSLLLFLQRTGEISVESVR
                     IGQGTGNNEDICDCLLSKD"
     gene            1237..1986
                     /locus_tag="P5219_03025"
     CDS             1237..1986
                     /locus_tag="P5219_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25760.1"
                     /translation="MTTDIELLSKKAGRQAALAEAGLADLPREADPAGRIEWVRLRPF
                     FTGDDIREMCDEALLRGYRCISLPLPWAAEAASLLHSAPVRVSTAVSFPLGTSPQEAK
                     VLETMSARMGENVDINVVLPVYRLAAGDKSGLRAEMENIREAAEGAVLSFYVETPMLD
                     DEKIISAVRSAEMARADRVIGGTGVFGFPTIRDVALLRCAAPDWMEVGTAVESTAKGA
                     RLLPFLVAAGAGSILAENPPLVLGAPETEKE"
     gene            2357..3241
                     /locus_tag="P5219_03030"
     CDS             2357..3241
                     /locus_tag="P5219_03030"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRX25761.1"
                     /translation="MLKEKTFWADTEFFGLLGNPVRKSLSPAMHNANFQALGMNAVYT
                     PYEVTESTVGTVIPALEALRFRGLNITMPLKQKIIQYLDELDEIASLCNAVNTVYWKN
                     GRLCGSNTDGLGFVRGLKEQGGYDLKGKQCLIFGAGGAARGVAFALAEAGIGSIALWK
                     RASGDEKLRKLASDLNAYRDGVCRIQSSDPADIPGLLKENELIINSTPLGMAPNTDST
                     PFDTSLLGTEHMVCDLVYVPHDTLLLRQAEEKGARTLYGYWMTIWQGVEAFRKWTGGK
                     EPDVEVMTKTMLEHLTKK"
BASE COUNT          795 a          917 c          936 g          699 t
ORIGIN      
        1 ggtgatgaag ggaggaaccc gcacgatttc ccggacatag ttcacgtcga gaccgaaagc
       61 ctcgtcccgg agagagaaaa caagggtaat gtgctccctc cccctgcttt ccaggtcctt
      121 ttgctgcata ttgccgtcat ttttcagcgt ttgcatccgt tttacctccc ggtgtttctg
      181 agtgaaaaga gcaggtcaga tccatggttc ctggtgcagg gtctccccgt tttccaggac
      241 gaaaatccgt atgccgtcac catcgagaac cgccgcgctt gccgggtcac ggccttttgg
      301 aagggcggcg gaaccgggat tgagaaagat ggtcccttcc tcccggacaa tggagcccac
      361 gtgggtatgg ccgtagatta caagatccgc cctgcatcga aggccaagct cccgcaaggg
      421 ctgaaagggg gcgccatgcc ccaccaggat gagcctcgaa tgccaccaga cggtggcata
      481 aggagccatc accggccatc cgagcagagc ctcgtcttcg ggacggtcac agtttccccg
      541 tgaaattata acaggaaagg gagcaccgcg aatcatctct tccaatcctg tggcgatgtc
      601 agcaaggaca tcaccgcagt gaagcacgcc atccacggat ccccatcggg agagggcttt
      661 cttccaggct tcttcgtggc cgtgggtatc ggatatgatg ccaagccttc cgttcattct
      721 cctccggacc tcccttttcc ctgttccctg gagggagact gagatacaat aatggaacga
      781 gcctgtgctg tttttcctgt attgtaccat tttcccattc ggggtggaaa cgcatctatt
      841 tgaatgaaag gaagcgaagg catgaacgaa catgacattt ttgaagaaat gaagaagctt
      901 cttgattctg aaggactggt aacagcccgg ctggtggaat ccgtcaccaa aacttcccag
      961 ttgacagcct ccaccgttcc cgagatccag gatcttttcc gccagtggct ttccctggta
     1021 gcccgggagg tcaagcgaat ggccgaaccg ggaaaggatc ttcccctggc cgggacagca
     1081 gggatgatcg gcatttctcc gtcttccctt ctctctcttc ttctgttcct tcaaaggacc
     1141 ggggaaatct ccgtcgaatc ggtgcgcata gggcagggaa cagggaacaa cgaggatatc
     1201 tgcgactgtc tcctgtcaaa ggactgacgg agactaatga cgaccgacat cgaacttctc
     1261 tcaaaaaagg ccggacgaca ggcggccctc gccgaggccg gcctggccga tctgccacgg
     1321 gaggctgatc cggcgggaag aatcgaatgg gtacggcttc gcccattctt caccggagac
     1381 gacatccggg aaatgtgcga tgaagcgcta cttcgcggct acaggtgcat cagcctaccc
     1441 cttccatggg cggccgaagc ggcctctttg cttcattcgg cacctgtccg ggtatccaca
     1501 gctgtttcct tccctcttgg gacctcaccg caggaggcga aggttctgga aaccatgtcg
     1561 gccaggatgg gggaaaacgt tgatatcaat gtcgttcttc ctgtctacag gcttgcggcc
     1621 ggagataagt caggattgcg ggctgaaatg gaaaatatca gggaggctgc tgagggagcg
     1681 gtcctgtctt tttacgtgga aacgccgatg cttgatgatg aaaaaatcat ttcagcggtc
     1741 cggtcggcgg aaatggcccg ggcggaccgt gtcatcgggg gaacgggggt tttcggattc
     1801 cccaccatac gggatgtggc cctgctgcgg tgcgccgctc cggactggat ggaagtcggt
     1861 acggccgtgg aatctacggc aaaaggagcc aggctgcttc cttttctcgt agccgcaggg
     1921 gccggaagta tccttgcgga aaatcctccc cttgtgctgg gagccccgga aactgaaaaa
     1981 gaataacctc cgtctgcctt tttcggggtc tttacttgcg gggaccatcc ctgacaccac
     2041 gaaaaacagg aagctgcatc tttcctgacc atgcccggca aagtcctgtc gaagccggta
     2101 caagggaatt ttcccggaaa tgttcctgat tgatgctgat taatgatgct gattaatgct
     2161 tgacatggag cttcgggtct gatatgtata gacaaaaccc gcttaatggt aagagttacc
     2221 gcagagcaga aaaaagaacc agatcacggt atttttcgag tcagtaaatg gaaccccggc
     2281 accgggacaa ccgcaccaca ggacaggaag gaccagaaaa atccaaatcg gccgaagaaa
     2341 aaggagcgtg cctttcatgt tgaaagaaaa gaccttttgg gcggataccg agtttttcgg
     2401 acttctcggg aatcccgtca gaaaatccct ctcccccgcc atgcataacg cgaatttcca
     2461 ggccctcggg atgaatgccg tctatacccc ctacgaggta accgaatcaa ccgtcgggac
     2521 agtcatcccc gcactggaag cactgcgttt caggggcctc aacatcacca tgccgctgaa
     2581 gcagaagatc atccaatacc ttgacgaact tgacgaaatc gcgtccctct gcaacgccgt
     2641 aaacacggtc tactggaaga acggcagact gtgcggttcg aacacggacg gtctgggatt
     2701 cgtccgggga ctgaaagaac agggcggata cgaccttaag ggaaaacagt gtctcatttt
     2761 cggcgccggc ggcgctgccc ggggagtggc tttcgctctc gccgaagctg gaatcggcag
     2821 catagcactc tggaagagag cgtcgggaga cgaaaagctc agaaaactcg caagcgatct
     2881 caatgcctac cgtgacgggg tctgcaggat tcagtccagc gatcccgccg acatccccgg
     2941 tcttctcaaa gaaaacgaac tgataattaa ctccaccccc ctgggcatgg ctccgaatac
     3001 ggactccaca cctttcgata cttccctgct cggaacggag cacatggtct gcgacctcgt
     3061 gtatgtcccc catgacacgc tgctgctgcg gcaggccgaa gagaaggggg cacggactct
     3121 ctacggctac tggatgacca tatggcaggg ggttgaagct ttccgaaaat ggaccggcgg
     3181 aaaggaaccc gacgtggagg tcatgacgaa aactatgctg gaacacctga cgaagaagta
     3241 aaaccagccc ctgaatgagg gattcattgc ccttgcggca tgaatccccg gaaaccaggc
     3301 cggaggtgaa ggttttgaaa ttactgaaat ggctggatga acatttc
//
LOCUS       DAOTHD010000119         3336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_33816_length_3336_cov_3.801890, whole genome shotgun sequence.
ACCESSION   DAOTHD010000119 DAOTHD010000000
VERSION     DAOTHD010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33816_length_3336_cov_3.801890"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2584
                     /locus_tag="P5219_03035"
     CDS             <1..2584
                     /locus_tag="P5219_03035"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HRX25762.1"
                     /translation="FFLLLSALHIQGWFEPAAASANRRVVLLHTLSPDDEWTRRATDG
                     IMESFSDSGVTAEIFIEYMDITSTFWSSHVERLKDLLEAKFSTSPPDVVVTTGNEAAT
                     FALVHTAVLFGGAPIVFCGLTNEYLFSVFPEGKGTGIYSVPAHNLLISEIGRLHPGAS
                     IAFVSDLTPAGLRDLSRLNTSLIQVESRPHVIPLSGFSADRLAESIRKLPDRTVIVLG
                     VHSSLPDGSPMTMKDTLALILRNTTLPVYTLDEDGIRHGALASVEGNGYEKGRKAGET
                     AVRILSGEAVNTIPPEKSMADHLTFDYPMMKVHSIPRNALSDRSVILNDPADAAVRYF
                     PVALLYMGLIVLLGGLAFFMKGKIAKRKRTEKNLTERTEYWEQLFQRSPEGIIVYNAA
                     GIVLETNLVFRSTFALTEENITEADIAGLLPWEKGTLRPEDFFPEGRGQIREVEIPDR
                     DGILIPATHLPFPLVSGTEKIYCSLVQDISQRKKIEEQLEKKGHFHEILSVITSRFIL
                     SPSYRDAMKSSLEDILALSKAKTAAIYRLSGRKGMLALETEARSAPEQPSLAGLFSSQ
                     QEEDFLWKSLLFRDGTFRPATLRPAELSQGERKEWLPLTQRGIATVSVLPLFLRDSFQ
                     GFLALADPSEEWISHSQEPVFDILRNSLAAAMERHQDELSLEKHHHAINERFTGVILA
                     LCQVSELRDVSTSGHQKNVSTLAEGLARRLGLPEDTVLGVRYAGLVHDIGKLYIPAEI
                     LSKPSKLTPAEYGLVKKHPEYGKDILSPLDFPWPLAEIILQHHERIDGTGYPGGLKKE
                     EIRVEARILSVADAFDAMISERPYRKQRSPAQALEEIRSLSGRAYDPEIVEVLEKHCR
                     DNTL"
     gene            complement(2628..>3336)
                     /locus_tag="P5219_03040"
     CDS             complement(2628..>3336)
                     /locus_tag="P5219_03040"
                     /inference="COORDINATES: protein motif:HMM:NF014818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRX25763.1"
                     /translation="HIDAGAERVIISAPSETADLCVVLGVNEDQYDPERHRVVSNASC
                     TTNSIAPAIKVLNDAFGIDYLMGTTIHAYTSSQKLVDVPKGGCRKNRAAGLSMIPAST
                     GAAKALIPLFPDLKGKMAMSAVRVPVADGSLSDLVVHLQKDANAGEVNEVLRRSAEER
                     LKGILEFSTEELVSSDIVGNSHSGIVDSLSTKMLMGRVAKIMIWYDNEYGYASRLLEL
                     ARYMAVKEPSMSFSSAR"
BASE COUNT          774 a          977 c          857 g          728 t
ORIGIN      
        1 tttttttctt ctgctgtccg ctcttcacat tcagggatgg ttcgaacctg cggccgcttc
       61 cgcgaacagg agagtcgtcc tgcttcacac cctttccccc gatgacgaat ggacccgccg
      121 ggcaacggac ggaataatgg agtccttctc tgactcagga gtcacggctg aaattttcat
      181 tgaatacatg gacataacga gcactttctg gtcgtcccac gtggaacggc tgaaagacct
      241 cctggaggcc aaattctcga cctcccctcc tgacgtggtg gtcactacgg gaaacgaggc
      301 cgccacattc gccctggtcc atacggctgt gctgttcggg ggggctccca tcgtgttctg
      361 cggactcacc aacgaatacc ttttttcagt gtttccagag ggaaagggaa ccgggattta
      421 ttccgttccg gcccacaacc tcctcatttc ggagatcgga aggctccatc caggggcatc
      481 catcgccttc gtttcagacc taacgcctgc agggctcagg gatctttccc ggctcaacac
      541 gagcctgata caagtggaat cccgccccca tgtgatccct ctctccggtt tttccgccga
      601 tcggcttgcc gaatcgatca ggaaactgcc ggacaggacg gtcattgtgc tcggggttca
      661 ttcttcactc cctgacggca gccccatgac gatgaaggat accctcgctc tcatactgcg
      721 gaataccacg ctgccggtct acacgctcga tgaagatggc atcaggcacg gcgccctggc
      781 gagcgtggag ggaaacggat acgaaaaggg cagaaaagcc ggagaaacgg ctgtcaggat
      841 tttgtctgga gaggcggtca ataccatacc gcccgaaaaa tccatggcgg accatctcac
      901 ctttgactac ccgatgatga aagttcattc aatccccaga aatgctctct cagaccgaag
      961 cgtcattctg aacgatcccg ccgatgccgc ggtcaggtat tttcccgtcg ctctcctcta
     1021 catggggctg attgtccttt tgggcgggct ggcgtttttt atgaagggaa agatcgcgaa
     1081 aagaaaaaga acggaaaaaa acctcactga aaggacggaa tactgggaac agctcttcca
     1141 gagatctccc gaggggatca tcgtatataa cgctgcagga attgttctgg agacaaacct
     1201 tgtcttccgt tccaccttcg cccttaccga agaaaatatc acagaagcgg acattgccgg
     1261 tcttcttccc tgggagaagg gaaccctccg ccctgaagat ttctttcccg agggcagggg
     1321 acaaatcagg gaggtagaaa tacctgacag agacgggatt ctcattcccg caacccatct
     1381 tcctttcccc cttgtttcgg gcacggaaaa gatctattgc tccctcgtcc aggatatcag
     1441 ccagagaaaa aaaatcgagg agcaactcga aaaaaaggga catttccatg aaatcctctc
     1501 cgtcataacc tcccgcttta tactctctcc gagttaccgg gacgccatga aatcttccct
     1561 cgaggatatc cttgccctgt cgaaagccaa aacggcggca atttacaggc tctcgggaag
     1621 gaaaggcatg ctggctctgg aaacggaggc cagatccgca ccggaacagc cttctctggc
     1681 ggggcttttt tcctcgcagc aggaagaaga ctttctctgg aaatcccttc ttttccggga
     1741 tggaaccttc aggcccgcca ccctccgccc ggcggagctt tcccagggcg agagaaagga
     1801 atggcttccc ctcacccagc gcggtattgc cacagtctca gttcttcccc tctttcttcg
     1861 ggactctttc caggggttcc tggccctcgc cgacccctcc gaggaatgga tatcccattc
     1921 tcaggaacct gtgttcgaca tactccgcaa ctccctggcc gcagcaatgg aacgccacca
     1981 ggacgagctc tccctggaaa agcaccacca cgctatcaac gaacgcttca caggggtcat
     2041 cctggccctc tgccaggtca gcgaacttcg ggacgtgtca acgtccggac atcagaagaa
     2101 cgtctcgacc ctcgcggaag gactggcccg aaggctcggg cttccagagg ataccgtact
     2161 gggtgtcagg tacgccggcc tcgtgcacga catagggaaa ctttatattc cggcggagat
     2221 tctgtccaag ccctcgaaac tcacaccagc cgaatacggt ctggtaaaaa agcatccgga
     2281 atacgggaag gatatccttt cacccctgga tttcccatgg ccccttgcgg aaatcattct
     2341 ccagcatcat gaaaggatcg acggcacagg atatcccgga ggattgaaaa aggaagaaat
     2401 tcgcgtcgaa gccagaatac tgtccgtggc ggatgccttc gacgccatga tatccgagcg
     2461 gccctaccgg aagcagcgct cccccgccca ggccctggaa gaaataagaa gcctgtcggg
     2521 aagagcttat gaccctgaaa tagtggaagt tctggagaaa cactgcagag acaacacgtt
     2581 ataatccggt gcaaagaggg cgtaccctgt gcacgccctc tttccgtcta acgggccgag
     2641 gaaaagctca tggaaggttc tttcaccgcc atgtaccgtg caagttcaag aagccggctg
     2701 gcataaccgt attcattgtc gtaccagatc attatctttg ccacccttcc catcagcatc
     2761 ttcgttgaaa gggaatccac aatgcccgaa tgggagtttc ccacgatatc cgaggatacc
     2821 agctcttccg tactgaactc gagaatgcct ttcagccttt cttcggcgga tctccgaagc
     2881 acctcgttga cttctccggc gttcgcgtcc ttttgaagat ggacgaccag atcgctgagg
     2941 gagccgtcag ctaccggaac ccggaccgcc gacattgcca ttttcccctt gagatctgga
     3001 aaaagaggga tgagcgcctt cgcggccccc gtggacgcgg gaatcatgga aagcccagcc
     3061 gccctgttct ttctgcatcc ccctttggga acatcaacca gtttttggga tgatgtgtag
     3121 gcatgaatgg ttgttcccat aaggtaatcg atgccgaagg catcgttcag cacttttatt
     3181 gcgggagcaa tggaattggt ggtacaggaa gcattggaca ccacccggtg cctttccgga
     3241 tcgtactggt cttcgttgac gccgagaacc acgcagagat cggctgtctc ggacggagcg
     3301 ctgatgatga cccgttcagc ccccgcgtct atgtgg
//
LOCUS       DAOTHD010000120         3329 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_33910_length_3329_cov_3.971900, whole genome shotgun sequence.
ACCESSION   DAOTHD010000120 DAOTHD010000000
VERSION     DAOTHD010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33910_length_3329_cov_3.971900"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="P5219_03045"
     CDS             <1..238
                     /locus_tag="P5219_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25764.1"
                     /translation="TIRMNGRSWRGQVRWQAMASNGTVTGSVWGFWNEMSNKVPFDFI
                     MAMIPDELAFRATYYVSPRKSGLPAENQGGDDFS"
     gene            235..900
                     /locus_tag="P5219_03050"
     CDS             235..900
                     /locus_tag="P5219_03050"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX25765.1"
                     /translation="MKKTVLLVFLVFSCICVSFRAEGAPPTPEQLYENGRRYELGKGV
                     SKNDSKAFSFYLKAAEAGHVEAQARTGLFYAFGRGTGENVKKALFWYEKAARQGHYGA
                     QNNLANILWTGPKGVKKDREKALDFWFLSAMHGYGEAQLKMALFYLSRGEQDPAAYRK
                     AYYWNFQAALQGLPEAQWWLGMLREAGKGVEKDMKTAVHWYKKAAAKGHREAKARLKK
                     LGY"
     gene            954..2396
                     /locus_tag="P5219_03055"
     CDS             954..2396
                     /locus_tag="P5219_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25766.1"
                     /translation="MKTMRIRGKWLKKSVLAAVCVLLGLGTGNRTVSAETATPINVLV
                     EMKVQWDITKKDMRYEGLLNVSAFGTMERTYGKGKQREGPAADAVAMFLPQSMAMRYS
                     YVEKETYVGPLHPGRCTARLRKSVVGRFSSEETEGGSLAIHRMSSATEAIVKNISPKG
                     KEFASQLLQFYQREPDSLSLSVKGPAKLRISNKLTGIRIRAGKTCEVERYEKAVPLFA
                     VGLRLTLPESGSVLGSHEWSTEGKGVPSYFDVSLSDTGGQMQEKPAAGGTGKVTFGQS
                     PRSEEGAATATYTVSWNIGENVKGPVDDDCEKLRDKIRWIEKVIETYEDEALREWFRL
                     NFGDGLKGMEEYQKQVEREVSEAFPDTKGNPAAIHSEFQTAPNCTTNECGAGENAPVI
                     GGIIDGESFNIMAFDSEGNLVSQDEALMNYFRGKTDGFDGIMDHEKQHVADLTSMGYP
                     KSIDEWAEFELRGFRKELAERKSKFERDCR"
     gene            2469..3044
                     /locus_tag="P5219_03060"
     CDS             2469..3044
                     /locus_tag="P5219_03060"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HRX25767.1"
                     /translation="MHCKSKGILRAALFLVFLLAGSALGAEFTADMVISSKAAGADMT
                     GKVFVKGNALRQELDTPVGVQTTIIPSGAAVMYVLLPGQKMYMEIPNTQVTLDSSENL
                     EAKMANQGKVTKKGSETIGGYSCDVYHIVYSDKNLGEGTVWVSGELNYPLKIFTVTPQ
                     DTATILYKNIRQGKLDNGLFSLPAGYKKFSM"
     gene            complement(3080..>3329)
                     /locus_tag="P5219_03065"
     CDS             complement(3080..>3329)
                     /locus_tag="P5219_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HRX25768.1"
                     /translation="IRPVDALSFGSVHDRPLLDIWNSPEFAAYRREIGTYPFPHCGNC
                     SLAPCDYIERHEFEQDCLGNRLTCGSCPWSLGVLQCMR"
BASE COUNT          881 a          801 c         1001 g          646 t
ORIGIN      
        1 taccatccgc atgaacggcc ggtcatggcg gggacaggtt cggtggcagg ccatggcgtc
       61 gaacggaacc gtaacagggt cggtatgggg attctggaat gagatgtcca acaaggttcc
      121 cttcgatttc atcatggcga tgattccgga tgagcttgcc ttccgggcga cctactacgt
      181 ttctccccga aaaagcggcc ttccggccga aaatcaggga ggcgatgatt tctcatgaaa
      241 aaaactgtcc tcctggtatt tctcgtattt tcatgcatct gcgtctcctt ccgggcggaa
      301 ggggcgcccc cgacgccgga gcagttatat gaaaacggca ggcgctacga gttaggaaaa
      361 ggagtctcca aaaacgattc aaaagccttt tccttctacc tgaaagcggc tgaagcgggg
      421 catgtggaag cacaggccag aacaggcctc ttctatgctt tcggaagggg aacaggagaa
      481 aatgtaaaaa aagccctctt ctggtacgaa aaagcggcaa ggcagggaca ttacggtgct
      541 cagaacaatc tcgcaaatat cctgtggacc ggcccgaaag gcgtgaaaaa agaccgggaa
      601 aaggcgctgg atttctggtt tctctctgcc atgcacggat acggagaggc acagctcaag
      661 atggcgctct tctatctcag caggggtgaa caggaccctg ctgcctacag aaaggcgtat
      721 tactggaact tccaggcagc cctccaggga ctgccggaag ctcagtggtg gctgggcatg
      781 ctgagggagg cgggaaaagg ggtcgaaaaa gacatgaaaa cagcggtcca ctggtataaa
      841 aaagccgccg cgaaggggca cagggaggca aaggcgcggt tgaaaaaact ggggtattga
      901 caaaagtcgt tcccttttca ccggcatcgg cagatcccat tggcggaaag agggtgaaga
      961 ccatgcgtat caggggaaaa tggctgaaga aaagtgtcct tgcggccgtg tgtgtccttc
     1021 tgggattggg tacaggaaac cgcacggttt ccgcggaaac tgccacgccg attaatgttc
     1081 tcgtcgaaat gaaggtccag tgggacatca cgaaaaagga tatgagatat gaaggcttgc
     1141 tcaatgtctc cgcattcggg actatggagc gcacgtacgg aaaagggaaa caaagggaag
     1201 gccccgccgc ggacgccgtg gcgatgttcc ttccgcagtc gatggccatg cgctattcct
     1261 acgttgaaaa agaaacctac gtggggcctc tccatcccgg aagatgcacg gcccgtctca
     1321 ggaaaagtgt cgtcgggcgt ttttcttccg aagaaaccga aggaggcagc cttgcaatac
     1381 acaggatgtc ctcggcaact gaagccattg tcaaaaacat atccccgaag gggaaggagt
     1441 ttgcttctca gctgcttcag ttctaccaaa gggaaccgga ttccctcagt ctttcggtca
     1501 agggccccgc gaaactcaga atatcgaata aacttaccgg gatccgcatc cgggccggaa
     1561 aaacctgcga ggtcgaaagg tatgaaaaag cggttccgct gtttgccgtt ggtctccgcc
     1621 tgacgctgcc tgaatcaggc agcgtactgg gaagtcatga atggtcgaca gaggggaagg
     1681 gtgtaccctc atatttcgat gtttcccttt cagacacagg cggacagatg caggagaaac
     1741 cggcagcagg cggaaccggc aaagtgacgt tcggacagtc gccccgttct gaggaaggag
     1801 ccgccacagc gacctacacc gtcagctgga acatcgggga aaacgtgaag ggacccgttg
     1861 acgatgactg tgaaaaatta cgcgacaaaa tcaggtggat cgagaaagtc attgagacgt
     1921 acgaggacga ggcgctcagg gaatggtttc gattgaattt tggggatgga ctgaaaggga
     1981 tggaagagta ccagaaacag gttgagcggg aagtcagtga agcgtttcct gatacgaaag
     2041 gcaacccggc ggctattcat tccgaattcc agacagctcc caactgcaca acgaatgaat
     2101 gcggagccgg tgagaatgcg cctgtcatag gaggtattat cgacggagag tctttcaaca
     2161 taatggcatt cgattccgaa ggcaacctgg tgtcacagga tgaagccctg atgaactatt
     2221 ttcggggaaa aaccgacggg tttgacggca taatggacca tgaaaaacag catgtggcgg
     2281 atctcaccag catggggtat ccgaagtcca ttgacgagtg ggctgaattt gaactgaggg
     2341 gattccggaa agagcttgcc gagcggaaaa gcaagttcga acgggactgc agatagaggc
     2401 gggaatgata caaagaacgg gaaagacgac aggaaccatt attgtcgcgg acaaaggaga
     2461 tgaagatcat gcactgtaaa tcaaaaggga ttcttcgggc ggcgctgttt cttgtttttc
     2521 ttcttgccgg ttctgctctt ggggctgaat tcacggcgga catggtcatt tcgtcgaaag
     2581 cggcgggagc agacatgacc ggaaaggtat tcgtaaaggg gaatgccctg agacaggagc
     2641 tggacacccc cgtcggtgtg cagaccacca tcatcccatc cggggcggcc gtgatgtatg
     2701 tgctgctccc gggacagaag atgtacatgg agatcccgaa cacccaggtg accctggatt
     2761 catcagagaa cctcgaggca aagatggcaa accagggcaa agtgacgaaa aaaggctccg
     2821 agaccatcgg gggctattcc tgcgacgtgt accatatcgt ctacagcgat aaaaacctcg
     2881 gcgagggaac ggtatgggtc tccggcgagc tgaactatcc cctgaagatt ttcaccgtca
     2941 caccccagga tactgccacg atcctgtaca aaaatatcag gcagggaaag ctcgataacg
     3001 gattgttctc gcttccggca ggatacaaaa agttctcaat gtaagacggt gaaggggcct
     3061 gtctgagcag gtcccttttt tatctcatgc actgcagcac cccgaggctc cagggacagg
     3121 agccgcaggt gagccggttc cccaggcagt cctgctcgaa ttcatggcgc tcgatgtagt
     3181 cacagggcgc caggctgcag tttccgcagt ggggaaaggg gtaggtgccg atctcacggc
     3241 ggtaggcagc aaactctgga ctgttccaga tgtcgagcag cggacggtca tggaccgatc
     3301 cgaaggaaag ggcgtccacg ggacggatc
//
LOCUS       DAOTHD010000121         3320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_34005_length_3320_cov_3.332619, whole genome shotgun sequence.
ACCESSION   DAOTHD010000121 DAOTHD010000000
VERSION     DAOTHD010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34005_length_3320_cov_3.332619"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="P5219_03070"
     CDS             <1..265
                     /locus_tag="P5219_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde oxidase"
                     /protein_id="HRX25769.1"
                     /translation="FDPRGKMTNSSFWDYKIFGTLDMPEMKTILAESDEPTGPYGAKS
                     ISEIGINGPAPAIANAIFDATGRRIYDLPLTPEKVLAAIKGVK"
     gene            262..1575
                     /locus_tag="P5219_03075"
     CDS             262..1575
                     /locus_tag="P5219_03075"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRX25770.1"
                     /translation="MTGKFGDSPDYVIGRGVVADGSGLFIEDGALLVSDGKVAEIGPW
                     ETVRKDDVPFYDVEGRLILPGFVNFHHHLYSFFAPGISPSGPTETFPDILENLWWRLD
                     RVIDEESLYYSVLMGALDSLSFGVTSVFDHHASMNLDSGSLDVAAEAVCQAGIRAVLC
                     LETSDRQGKEAMKRQVAENLSFWEKHRSDPFLGGMFGLHANLTLSEETLSFISEEKPP
                     EMAVHVHCGEAPEDLAFCREQGYEGPVDRLNSFGLLDGDSFLIHCVHLSEKDYSLLGE
                     IRPVVVLNPESNANNRVGKPDRDRLPGHLLGTDGMSGDLVAALRSYLLLGAGGGEPYS
                     RMADMMFRYPQKILQKHLQVRTGFRPGAAADIAVLDYIPLSPVSEANLLGHILFGAKG
                     GKAHLTAVNGKILWRKGEFPGHDMNSLRFRAKRAASAQSLRFRNL"
     gene            1594..2517
                     /locus_tag="P5219_03080"
     CDS             1594..2517
                     /locus_tag="P5219_03080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRX25771.1"
                     /translation="MADLSSRVHDVFFSNPVLTAAGPNVGTAVRLQEAVAGGAGGIVT
                     KTVSVVPAKDPRPTIRKASCGGLLNCETWAETPVEEYLEAYREVKRAGVPLIASIGYS
                     PEDVRTLGRLLEKEVGPDIIEFSTHYTGKSLDPLLDVAKALREAVSVPVWMKISPNTP
                     DIEELAVRAADIVDGFVAINSFGPALDFDIDTAKPPLGSTYGQGWLSGPPIRPIALRI
                     VYQIASVQPKPVIGVGGICTGRDALQFIMAGASLVQICSAAIQNGPEIYGKVAREMGE
                     WMDSSGIASIEDVRGQYLRNVMARNARSGLK"
     gene            2534..>3320
                     /locus_tag="P5219_03085"
     CDS             2534..>3320
                     /locus_tag="P5219_03085"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRX25772.1"
                     /translation="MDETTAVVGSPVPRTDGLAKACGSAQFLDDLNIDGCWIGGTVRA
                     QVPRGKLREIRLDPSFDWGRVVLVTARDLPGINAVSMIRDDYPALADLLISFPTQAVA
                     LVAAPDQETLGEALAAVSLDVEELPPILTVEDSIACTQVIWGENNIMDEYSIASGNAA
                     EAFETADLIVEGTYRTKHQEHVYLETQGMAALPGEDGSLEVIGSMQCPYYVHSALVKA
                     LGFPPEKVRVRQTVTGGAFGGKEDYPSILGLHAALLALKSGRPV"
BASE COUNT          685 a          938 c         1000 g          697 t
ORIGIN      
        1 cttcgacccg agggggaaga tgacgaacag ctccttctgg gactacaaga ttttcggtac
       61 cctggacatg ccggaaatga agaccatcct ggcggaatcg gacgagccta cgggccccta
      121 cggtgccaaa tccatttccg agataggcat caacggaccg gcgcctgcca tcgccaacgc
      181 catttttgac gccacgggca gaagaatcta tgaccttccg ctgacccccg agaaggtgct
      241 tgcggcgatc aaaggtgtca aatgaccggg aaattcggtg acagcccgga ctacgtcatc
      301 gggcggggtg tcgtggccga cggatcgggg ctgttcatag aagacggagc cctgctcgtc
      361 tccgacggca aagtcgcgga gatcggaccc tgggaaacag tcaggaagga cgacgttccc
      421 ttttacgacg tggaaggacg gctgatcctt ccgggattcg tgaatttcca ccatcacctg
      481 tattctttct tcgccccggg aatctctccg tcgggtccga cggagacctt tccggatatt
      541 ctcgagaatc tctggtggag gctggacagg gtgatcgacg aggagtcgct ctattattca
      601 gtacttatgg gagccctgga ttctctctcc ttcggggtga cctctgtttt tgaccatcac
      661 gcttcaatga accttgacag cggaagcctc gacgtagccg ctgaagccgt ttgccaagcg
      721 gggatccggg cagtgctctg cctggaaaca tcggaccgtc agggaaaaga ggccatgaag
      781 cgccaggtgg cggagaacct ttccttctgg gagaagcatc gcagcgaccc ctttcttggg
      841 gggatgttcg gtctccacgc caacctgacc ctttccgagg agaccctctc cttcatttca
      901 gaggaaaaac cgccggaaat ggcagttcat gttcattgcg gggaggctcc ggaagatctt
      961 gccttctgcc gggaacaggg gtatgagggc ccggtggacc gcctgaactc cttcggtctc
     1021 ctggacgggg acagtttcct gatccattgt gtccatctct cggagaagga ttattccctt
     1081 ctcggggaga tcaggcccgt ggttgtcctg aatcctgaat ccaatgcaaa caaccgggtc
     1141 ggaaagccgg acagggacag gcttcccggt catcttctcg ggacggacgg aatgtcggga
     1201 gacctggtgg ccgctctccg ttcctacctg ctcctcggag ccggaggcgg tgagccctat
     1261 tccaggatgg ctgacatgat gttccggtat ccgcaaaaaa tacttcagaa gcaccttcag
     1321 gtacggacgg gattccggcc tggagctgca gcggatattg cggttctcga ttacattccc
     1381 ctttctccgg tttcagaggc aaaccttctc ggccacatcc tcttcggtgc aaagggaggg
     1441 aaagctcatc tcacggcagt caacggcaaa atcctctggc ggaagggaga gtttccaggc
     1501 cacgacatga attctctccg gttccgggcc aaaagggcgg catctgccca atcgctccgt
     1561 tttcgaaatt tgtgagttca gggaggcgaa atcatggcgg atctttcttc tagggtacat
     1621 gatgtctttt tttccaatcc ggtgctgaca gccgcaggtc cgaatgtggg tacggccgtc
     1681 aggctgcagg aagccgtggc cggcggagcc ggaggaatcg tcacaaaaac ggtttccgtg
     1741 gttcccgcga aggacccgag gcccaccata cgaaaagcct catgcggcgg ccttctgaac
     1801 tgtgaaacct gggccgagac gccagtggag gagtacctgg aagcctaccg ggaggttaag
     1861 cgggccgggg ttcccctcat cgcgtccatc ggctactcgc cggaggatgt acgaaccctg
     1921 ggcaggctgc tggaaaaaga agtcgggccc gacatcatcg agttttccac ccactatacg
     1981 ggaaaatccc ttgatcctct cctggacgtg gcaaaagccc tccgggaggc tgtatccgtt
     2041 cccgtctgga tgaaaatttc tcccaatact ccggatatcg aggagctggc agtaagagct
     2101 gcggacatcg tggacggttt tgtggccatc aattccttcg ggccggccct ggatttcgac
     2161 attgacacgg caaagcctcc actgggctcg acctacggcc aggggtggct ttcgggacct
     2221 cccatccgtc ccattgccct ccgcattgtc taccagatag catcggtcca gccgaagccc
     2281 gttatcggcg tgggaggaat ttgcaccggc agggatgccc ttcagttcat catggcagga
     2341 gcctccctgg tgcagatctg ttccgcggcc atacagaacg ggccggaaat ttacggaaag
     2401 gtggcccggg agatgggaga gtggatggat tcctctggaa ttgcctccat tgaggacgtc
     2461 cggggccagt acctgagaaa cgtgatggcc aggaacgccc gatcgggtct gaagtagaaa
     2521 ggaggagccc ctgttggatg agacaacggc cgtagtgggt tctccggttc cgagaaccga
     2581 tggcctggca aaagcgtgtg gtagtgcaca gttcctggac gacctgaaca tcgacgggtg
     2641 ctggatcggc ggaacggtgc gcgctcaggt gccgaggggg aaattacggg aaatccggct
     2701 cgatccatct tttgactggg gccgtgtcgt tctggtgaca gcccgggacc taccgggaat
     2761 caatgcggtt tccatgatcc gggacgatta tcctgccctt gccgaccttc tcatttcttt
     2821 cccgacccag gcggttgccc tggtggcggc tccggaccag gaaacgctcg gagaagccct
     2881 tgctgcagtt tcgctggacg ttgaggaact gcctcccatc ctcaccgtgg aggactctat
     2941 cgcctgcacc caggtcatct ggggcgaaaa caacatcatg gacgaatatt cgattgcctc
     3001 cgggaatgcc gcggaggcat tcgaaaccgc cgacctcatc gtggagggga cctacaggac
     3061 aaaacaccag gagcatgtct atctcgaaac ccagggaatg gcggcccttc ccggggagga
     3121 cggttccctg gaggtcatcg gttccatgca gtgtccctat tatgttcact ccgccctggt
     3181 caaggctctg ggctttcctc ccgaaaaggt cagggtacgc cagactgtta cagggggagc
     3241 tttcggaggg aaagaggact atccgtccat tctcgggctc cacgccgctc ttctggcact
     3301 gaagagcgga cggccggtaa
//
LOCUS       DAOTHD010000122         3313 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_34089_length_3313_cov_2.230203, whole genome shotgun sequence.
ACCESSION   DAOTHD010000122 DAOTHD010000000
VERSION     DAOTHD010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34089_length_3313_cov_2.230203"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="P5219_03090"
     CDS             <1..795
                     /locus_tag="P5219_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRX25773.1"
                     /translation="VGKTELAKTLAEALFDSEDNLVQIDMSEYMEKHSVSRMVGAPPG
                     YVGYDEGGQLTEAVRRKPYSVLLFDEIEKAHPDVFNILLQILDDGRITDSRGHVVNFK
                     NTVIIMTSNIGAPLLLQGITSEGEIRENARDGVMEELRRSFRPEFLNRVDDVVLFKPL
                     TPAEIGRIVRLLASALSDRLRERNISLEITDRGAAFIADAAYDPVYGARPLKRYMVRN
                     IETPVARMIISGEAGEGMVITVDEGEGKLVLRTAPSGEEDVQPTNS"
     gene            816..1136
                     /locus_tag="P5219_03095"
     CDS             816..1136
                     /locus_tag="P5219_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25774.1"
                     /translation="MAAVKIEPVRSGGILPHLPAEDGGADREEALKKVEKARREVEKE
                     RLDIMDRHHETVKKSAETYDKLVKKKLLEEEIEKRRLLQREYLEERAEDAGLRRKQGA
                     DRTD"
     gene            1295..2485
                     /locus_tag="P5219_03100"
     CDS             1295..2485
                     /locus_tag="P5219_03100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRX25775.1"
                     /translation="MTSKTFRLFRTESSGFKLSLLSAGHFFNDLYASFLPTFIPTLIS
                     RLGLTMAQAGFFSTFLGVIHIIFQPVIGYLSDRSANPWLIIWGPILTCFGATMIPLSP
                     TYGTALLFVGLWGLGSAMFHPQGHGGVGHVVPRNRLTVSLALFAVAGTAGVTMSPLFA
                     VALVNTVGLKLMPVAAILPVLILGLFTWRTMPSISHESGDAMTPQKGLFSTMKSVFAV
                     IYPIWAMSTVRDAASQGVRMFFPIRIAGEGGDISFVGTVLFLIMLGSTIAMLVIGRMA
                     DRYGKKQTLTVTMALSSLFLFAGRASGGWTAILFFVLGTAAVNATMPITAAIAQEMVP
                     NSRGMASSIVMGLSWGMGNMLMAPFGKVGDLYGVNATLFIVALLPLLTLPLLLTRPFR
                     EAKN"
     gene            complement(2541..>3313)
                     /locus_tag="P5219_03105"
     CDS             complement(2541..>3313)
                     /locus_tag="P5219_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HRX25776.1"
                     /translation="GDPLTLSDGTLDFILSRLRAIPTVEIIRIGTRIPAVLPMRVTEG
                     LCSVLKKYHPLWINLHFNHPKELTPEAAAACAKLADAGIPLGNQSVLLKGVNDCPYIF
                     RDLNQKLLKMRVRPYYIYQCDLSRGIEHFRTSIGKGIEIIEYLRGHTSGLAVPTFVVD
                     APGGGGKIPVMPNYVLSRSDRKTILRNFEGVVTVYTEPDDNRSVCGGKCRETCERAKS
                     LYKGGMESLFEGEAVSLEPRELAREKRRKRWAEGEKST"
BASE COUNT          633 a         1003 c          999 g          678 t
ORIGIN      
        1 gtgggcaaga ccgagctcgc caagaccctt gccgaggccc tcttcgacag cgaggacaat
       61 cttgtccaga ttgacatgtc ggaatacatg gagaagcact ccgtctcccg catggtgggg
      121 gcgcccccgg ggtacgtggg gtacgatgag ggcgggcagc ttaccgaggc ggtcaggagg
      181 aagccctatt cggtgctcct ctttgacgag atcgaaaagg cccacccgga cgtgttcaat
      241 atcctgctcc agattctcga cgacggcagg atcaccgaca gcagggggca cgtggtgaac
      301 ttcaagaaca cggtcatcat catgacgagc aacatcgggg ctccccttct gctccagggc
      361 atcacctccg aaggcgagat ccgggagaat gccagggacg gcgtgatgga ggagctgcgg
      421 cggtccttca ggccggagtt cctgaaccgg gtggacgacg tggtcctctt caagcccctc
      481 actcccgccg aaatcgggcg gattgtccgc ctgctggcct cggccctgtc ggaccgcctc
      541 agggagcgga acatctccct ggagatcacg gaccggggag cggccttcat cgccgacgcg
      601 gcctacgatc ccgtctatgg cgcacggccg ctgaaacggt acatggtccg gaacatcgag
      661 acaccggtgg ccaggatgat catctccgga gaggccgggg aaggaatggt catcaccgtg
      721 gacgaagggg aggggaagct ggtgcttcgg acggctccct caggtgagga agacgttcag
      781 ccgacaaata gctaaagacc tttcggaaag ggaggatggc ggcagtgaaa atcgaaccgg
      841 tccggagcgg ggggatcctt cctcatcttc ctgcggagga cggcggagca gacagggagg
      901 aagccctgaa aaaggtggaa aaggccaggc gggaagtgga gaaggagcgg ctcgacatca
      961 tggaccgcca tcatgagaca gtgaaaaaat ccgccgagac ctacgacaag ctggtaaaga
     1021 aaaagctcct tgaggaggag atcgagaaaa gacggctcct ccagagggag tacctcgagg
     1081 aacgggccga ggatgccggg ctccggagaa agcagggagc tgacaggacg gactgaccgg
     1141 gtattctgtg aaaagaccgt ttcatcggcc ggagggcggg acaccgcctt ccggtttttt
     1201 gcattatgta tttttaacgc ccgaaagtat tacagattcc ttcattcgtg gtactatttg
     1261 cctcgatcat tatccgaagg agtgaccttt ctttatgacg tcaaaaactt tccgcctctt
     1321 ccgcaccgag agctccgggt tcaagctgtc cctgctctcg gcggggcatt ttttcaacga
     1381 cctgtacgct tcctttcttc ccaccttcat tccgaccctc atttccaggc tgggactcac
     1441 catggcccag gccggctttt tcagcacctt cctcggcgtg atccatatca tcttccagcc
     1501 tgtcataggc tacctttccg accgctcggc caacccgtgg ctcatcatct ggggtcccat
     1561 cctcacctgt ttcggagcca ccatgatccc cctgtcgccc acctacggaa cggctcttct
     1621 cttcgtcggg ctctgggggc tgggaagcgc catgttccac ccccagggac acggcggagt
     1681 ggggcacgtt gttccccgga acaggctgac cgtctcactg gccctcttcg ccgtggccgg
     1741 gaccgccgga gtgacgatga gccccctctt cgccgtcgca ctggtgaaca ctgtgggcct
     1801 gaagctcatg cccgtggcgg ccattcttcc ggtgctcatc ctcgggcttt tcacatggag
     1861 gaccatgccc tccatctccc acgagagcgg cgatgccatg acgccccaga aagggctctt
     1921 ctccaccatg aaatccgttt tcgccgtcat ttaccccatc tgggccatgt ccaccgtgag
     1981 ggatgccgca agccagggag tgcggatgtt ctttcccatc cgaatcgcag gtgaaggcgg
     2041 ggatatctcc tttgtgggca ccgttctctt cctcatcatg ctgggaagca ccatcgccat
     2101 gctggtgatc ggcaggatgg ccgacaggta cggcaaaaag cagaccctga cggtcaccat
     2161 ggcactctcc tccctctttc ttttcgctgg gcgggcatcc gggggatgga ccgccatcct
     2221 cttcttcgtc ctggggacgg cggccgtgaa cgccaccatg cccatcacgg cggccatcgc
     2281 ccaggagatg gtccccaatt cgcggggaat ggccagctcc atcgtcatgg gcctgtcctg
     2341 gggcatgggg aacatgctca tggcaccctt cgggaaggta ggagacctct acggggtgaa
     2401 cgccacactg ttcatcgtgg cgctccttcc cctcctcact cttcccctgc ttctcacccg
     2461 ccccttcagg gaagcgaaaa actgaatacg tccgatacag tgaaaatggc gaagggcgcg
     2521 gaagattccg cgcccttcgc ttatgtgctc ttttcgcctt cagcccagcg ttttcttcgc
     2581 ttttcccggg cgagttcccg gggttccagg gaaaccgctt ctccttcgaa cagggattcc
     2641 attcctcctt tatagaggct ctttgcccgt tcgcaggtct ccctgcactt accgccgcag
     2701 acgcttctgt tgtcgtccgg ctccgtgtag acggtgacga ctccctcgaa gttccggagg
     2761 attgttttcc tgtccgacct ggagaggacg tagttcggca tgacggggat cttcccgccg
     2821 cctcccggcg cgtccaccac gaaggttggc acggcaagac ccgacgtgtg tcccctgagg
     2881 tactcgataa tttcgatgcc cttgccgatg gaggtgcgga aatgctctat gccccgggag
     2941 aggtcgcact ggtatatgta gtaggggcgc acccgcattt tcaggagctt ctggttgagg
     3001 tcccggaaga tgtagggaca gtcgttcacc cccttgagga ggacggactg gttgccaagg
     3061 gggattccgg cgtcggccag cttcgcgcat gcggcagccg cctcgggagt caattccttg
     3121 gggtgattga agtgcaggtt gatccagaga gggtggtact ttttcagcac ggagcagagc
     3181 ccctcggtca ccctcatggg cagcacggcg ggtatcctgg tgccgatgcg gatgatctcc
     3241 accgtgggta tggcccgcag cctcgacagg atgaaatcga gggtcccgtc cgacagggtg
     3301 agggggtcac ccc
//
LOCUS       DAOTHD010000123         3276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_34586_length_3276_cov_2.976715, whole genome shotgun sequence.
ACCESSION   DAOTHD010000123 DAOTHD010000000
VERSION     DAOTHD010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34586_length_3276_cov_2.976715"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /locus_tag="P5219_03110"
     CDS             complement(<1..1125)
                     /locus_tag="P5219_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HRX25777.1"
                     /translation="MVRSVCIHGHFYQPPRENPWLGEIEIQESAAPWHDWNERVAAEC
                     YGPNGAARILDDEGWIRSIVNNYSRISFNFGPTLLSWMERKRPDIYEAVLRADGLGTA
                     RFSGHGPALAQVYNHMILPLADRRDKETQVIWGMEDFRARFGRDPEGMWLAETAVDTE
                     TLEVLAENGIAFTILAPRQAAAVRPAGADAWAGITGERVDVFRPYRCPLPSGKSMVLF
                     FYHGGIAQQVAFGETLRNGSAFASMLLDTILASSAESPLLSVATDGETFGHHHKYGDM
                     ALAYCLDRLERSGRVNLTIYGEYLEKHPPADEVRIVENSSWSCAHGVERWRSDCGCSA
                     HSRPGWGQGWRKPLREALDGLRDTLSPLFGEEGASVLPDPY"
     gene            1246..2739
                     /gene="malQ"
                     /locus_tag="P5219_03115"
     CDS             1246..2739
                     /gene="malQ"
                     /locus_tag="P5219_03115"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRX25778.1"
                     /translation="MFFLNRACGILLPVPSLPGGYGSGDLGKPAHDFVDFLSRSGQTI
                     WQVLPLTVTDGALGNSPYSSPSAFAGNPLCISPDDLVARGLLLRDELPGHPPFSEDRV
                     DYAAARIFKEHILRLAFSRFSPDGNYEAFLREQEHWLEDYVLFTALKGVYNGDPWNCW
                     PEKIRRRDGNALLAASRDLGEEPDFLRFVQYIFFSQMKDLRAKCREAGVELIGDLPIY
                     VNYDSADVWSRQPLFCLDQNLLPTEVAGVPPDYFSETGQLWGNPLYNWDTLKKDNFSW
                     WIRRLSHSLSQFDTVRIDHFRGLLAYWAVPFGDKTAERGTWRPVPSSDFFEAIRRNLP
                     SPSFLAENLGVITPDVTEAMESMGFPGMAVALFAFGGGMRDNPHIPHNYRRELAAYTG
                     THDNNTIEGWYSEDATGDELQAFREYTGRSAGGESAADTIIRLVLSSVAERAVVPLQD
                     YLGLGSEGRMNTPSVPSGNWEWKARKEHLSEDLSRRMGNLADIYGRK"
     gene            2813..>3276
                     /locus_tag="P5219_03120"
     CDS             2813..>3276
                     /locus_tag="P5219_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HRX25779.1"
                     /translation="MTSMHQRELGSSIRKLMESDPAFRPVAYLSMEIGIKESLPTYSG
                     GLGILAGDILKSAADLGVPMVGLTLLYRKGYFEQSFNADGWQTEKPVQWQPVQELTLL
                     PNQVSLTLQNREVHVRVWQYEIIGATGYPLPVYFLDTDFDNNHPDDRKLTWHL"
BASE COUNT          674 a         1003 c          961 g          638 t
ORIGIN      
        1 gtaagggtcg ggaaggacgg atgccccttc ttcaccaaaa agaggggaaa gggtatcccg
       61 aaggccatcc agggcttccc tgaggggctt tctccagccc tggccccagc ccggcctgga
      121 atgggcggag cagccgcagt cgctcctcca ccgctcaacg ccgtgggcgc agctccatga
      181 ggagttttcc actatcctga cctcgtccgc tggaggatgc ttttccaggt attccccgta
      241 gatggtgagg ttcacgcggc cgctccgctc gagccggtcg agacagtatg caagagccat
      301 atcgccgtac ttgtgatggt ggccgaaggt ctccccgtcg gtggccacgg agagaagggg
      361 ggactcggcg gaagaggcca gaatggtatc cagcagcatg gaggcgaagg cacttccgtt
      421 ccggagcgtt tcaccgaagg ccacctgctg ggcgatgccg ccgtggtaga aaaacaggac
      481 catggatttt cccgacggga gagggcaccg gtacggccga aaaacatcga cccgctctcc
      541 cgtgatgccg gcccaggcat ctgcgcctgc gggcctcacg gcggcggcct gccggggagc
      601 gagaatggtg aaggcaatgc cgttttcagc caggacttca agggtttcag tgtcaacagc
      661 cgtctcggcg agccacatgc cctccggatc cctgccgaag cgggcccgga agtcctccat
      721 cccccagatg acctgggttt ccttgtccct ccggtcggcc agggggagga tcatgtggtt
      781 gtacacctgg gcaagggcag gtccgtggcc ggaaaatcgt gccgtaccca ggccgtcggc
      841 ccgcagaacg gcctcgtaga tgtcaggacg ctttctttcc atccaggaaa ggagggtggg
      901 accgaaattg aagctgatgc gggaataatt gttgacgatg ctccggatcc atccttcatc
      961 gtcgaggatt ctcgcggccc cgttcgggcc gtagcactcc gccgcgaccc gttcattcca
     1021 gtcgtgccag ggggcggccg attcctgaat ttcgatctcc ccgagccagg gattctccct
     1081 ggggggctgg tagaaatgac cgtgaatgca cacagaacgg accataggga cactcctctc
     1141 cccttgcgct ttgtggtcaa ttatatatca tgaccgcccc gaaaggcggc gcgggactgc
     1201 cttcctggtg tagaatggca ctgagagcgg caactgacta cggaggtgtt tttcctgaac
     1261 agagcatgcg gcatccttct tcccgtcccg tcccttccag gagggtacgg atcgggagac
     1321 ctgggaaagc cggcccatga tttcgttgat ttcctttccc gttccggcca gaccatctgg
     1381 caggttcttc ccctcaccgt gacggacggc gccctcggca actcccccta cagctcaccg
     1441 tcggctttcg cgggcaaccc cctgtgcata tccccggatg atcttgtcgc gcgggggctg
     1501 ctcctcaggg acgaactccc gggacatccc cccttttccg aggaccgggt tgattacgcc
     1561 gccgcaagga tattcaagga acatatcctg cggctcgcct tttcccgttt ttcgcccgac
     1621 gggaattatg aagccttcct cagggaacag gaacactggc tcgaagacta tgtcctcttc
     1681 acagccctca agggggtcta caacggagat ccctggaact gctggcccga gaagatccgc
     1741 cggagggacg ggaatgccct tctcgccgca tcccgggatc tcggggaaga gcccgacttt
     1801 ctccggttcg tccagtacat tttcttttcc cagatgaagg acctgcgggc aaaatgcagg
     1861 gaggccggag tggagctcat cggggacctt cccatctacg tgaactacga cagtgccgac
     1921 gtctggtcga ggcagcccct tttctgcctc gaccagaacc ttctgccgac cgaggtggcg
     1981 ggggttcctc cggattactt cagcgaaacg gggcagctct ggggaaaccc tctctataac
     2041 tgggacactc tgaaaaagga caacttttcc tggtggatca gaagactcag tcattccctc
     2101 tcccagttcg acaccgtgag aatcgaccat ttcaggggtc ttctcgccta ctgggccgtt
     2161 cccttcggag acaaaactgc ggaacgggga acctggagac cggttccctc gtcggatttt
     2221 ttcgaggcaa tcaggagaaa tttgccttct ccgtccttcc tggcggagaa ccttggggtg
     2281 atcacccctg acgtcacgga agccatggag tccatggggt tccccggaat ggcggtggcc
     2341 cttttcgcct tcggcggcgg aatgagggac aacccccata tcccccacaa ctaccggcgg
     2401 gaactggccg cctataccgg aacccacgac aacaatacca tcgaaggatg gtattccgaa
     2461 gacgccaccg gggacgaact gcaggcattc cgggagtata ccggccgttc cgcaggcggg
     2521 gaaagcgccg cggacacgat catccggctc gtcctttcct ccgtggccga gcgggctgtg
     2581 gtgccgctcc aggactacct gggactgggc tctgagggaa gaatgaacac cccgtcggtc
     2641 ccctccggga actgggaatg gaaagccagg aaggagcacc tgtcggaaga cctctcccgg
     2701 aggatgggga acctcgcgga tatctacgga agaaagtagc cgcccctctc caagaaagga
     2761 aaactttctg gtaatattaa gacagtaaag agagggagga attattctca tcatgacatc
     2821 catgcatcag agagaactgg gaagctccat acgaaaactc atggaaagcg accctgcttt
     2881 ccggccggtg gcctatcttt ccatggaaat cggcatcaag gaatcactcc ccacctattc
     2941 aggcggcctc ggcatccttg ccggggacat cctgaaaagc gctgcggatc tcggcgtgcc
     3001 catggtgggg ctgacattgc tctacaggaa gggatacttc gagcaatcct tcaacgcaga
     3061 cggctggcag acggaaaaac cggtgcaatg gcagcctgtc caggagctta ccctccttcc
     3121 gaaccaggtc tccctgactc tccagaacag ggaagttcac gtccgggtat ggcagtacga
     3181 gatcatcggg gccacgggct accctctccc cgtctatttc ctggacacgg acttcgacaa
     3241 caaccacccc gacgaccgaa agctcacctg gcatct
//
LOCUS       DAOTHD010000124         3272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_34636_length_3272_cov_2.961144, whole genome shotgun sequence.
ACCESSION   DAOTHD010000124 DAOTHD010000000
VERSION     DAOTHD010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34636_length_3272_cov_2.961144"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="P5219_03125"
     CDS             complement(<1..880)
                     /locus_tag="P5219_03125"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRX25780.1"
                     /translation="MTDMPSAAFFRCGSYERDEVEDRMGKLLESLGGMERFVRPGESV
                     LLKPNLLSASSPERCITTHPEVVRAAALRVQAAGGHPFIGDSPGLDGFSSVGAKTGME
                     DVAAELGIPCVELDDPVPLPMAEGSVFQRVEVSRKALEADKIINLPKLKTHAQMMLTM
                     GVKNLFGTVVRQRKASWHYTVGLDRDVFADLHLDIARSLAPALTIMDGIGGMEGRGPG
                     NGRPRMFGLLAASADPLELDLRLCTLLGADLQDFPLYRSAQRRNLLPKDFGTTAGDFP
                     PDFVFEKVDIPRLDSLH"
     gene            1014..1748
                     /locus_tag="P5219_03130"
     CDS             1014..1748
                     /locus_tag="P5219_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25781.1"
                     /translation="MAYASQQFFLQKREVGLIAPVLCTLRILGEDDIMEAVTFHRRIT
                     DEIGDAGFFAEDNTIVESIRGEGAVSGIFAEGRLIALRAVSYVKEYVDDALEDLGLDQ
                     SEANNTAVMDFCVTDSAFRGNSIQFFTYLFMENILYPNRYHLHTTVSPKNIFSLTNIL
                     KCGFHAMRFKSKYGGHPRFVLYKNLRKPMAVQTRGHKEILLRNYDYHPKVFADGFVGY
                     KVKHKSNGMALLYGKPLVVREEPKAS"
     gene            1745..2959
                     /locus_tag="P5219_03135"
     CDS             1745..2959
                     /locus_tag="P5219_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25782.1"
                     /translation="MKDLLSGAGEILRRLEEEGGEAVIVGGMVRDLLCGFPPCDADIA
                     SDAPLPVLASLFPSGRILGPEGMQVFLLPFGEGHCEVFPFPRGGLRDDLARRDFTVNA
                     LALRRTGEVEGSRRALADIAARVIRFNGPGAERLAEDPLRALRLARFAAVLPGFVPDP
                     GAIRECREARPSLERCAPERVGREIRLGLEGNSRIFLETVKACGLLDVLFPGMPSREF
                     DFSRLCRIQEGLAREGAPLEVRAAALFPVPGGRPSRSDDGPSRAEETLLAWKWSARSA
                     SEAAELARQRRLPLEAPDPERTAALLEARGTPFMDSLFLLSRQYCAGEHHFRRWSENR
                     ALYVSMAVRALRNDVLPSGAEIMKKFSLAPGPLIGELADAVKLRRLHPGLGSKEDAFR
                     FLETLLRQNRNP"
     gene            3062..>3272
                     /locus_tag="P5219_03140"
     CDS             3062..>3272
                     /locus_tag="P5219_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25783.1"
                     /translation="MKKAAPWFVLLFFLAVPMAAPPGEAEAAPAITETYLASSVKEDG
                     APVGRVSAYDPSAKEFVAVAEVRDAH"
BASE COUNT          623 a         1005 c         1040 g          604 t
ORIGIN      
        1 agtgaagaga atcaagcctg ggaatgtcaa ccttttcaaa aacgaagtcc ggcggaaagt
       61 ctccggcagt tgttccgaag tctttcggca gcaggttccg ccgttgtgct gagcgataca
      121 ggggaaagtc ctggagatcg gctccaagga gggtgcagag gcgaaggtcc agttcaagcg
      181 gatcggcgga ggcggcgaga agcccgaaca tgcggggccg tccgttcccg ggaccacggc
      241 cttccatgcc cccgatgccg tccatgatgg tgagggcggg ggcgagactt ctcgcgatgt
      301 ccaggtgaag gtcggcgaac acgtccctgt caagcccgac ggtgtagtgc cacgaggcct
      361 tccgctgccg gaccaccgtt ccgaagaggt ttttgactcc catggtgagc atcatctggg
      421 cgtgggtctt gagcttgggc aggttgatga ttttgtcggc ttcgagggcc ttcctggaga
      481 cttccacccg ctggaagacg gatccctccg ccatgggaag ggggacaggg tcgtcaagct
      541 cgacgcaggg tatgcccagt tccgcggcga cgtcctccat gccggtcttc gcccccacgg
      601 aggaaaaacc gtcgaggccc gggctgtctc cgatgaaggg atgtcccccc gctgcctgta
      661 ccctgagggc cgcagcccgg accacctcgg ggtgagtggt gatgcatcgt tccggagaag
      721 aggcggagag gagattgggc ttcaggagca cactctcccc ggggcggaca aaccgttcca
      781 ttccccccag ggactccagg agcttcccca tgcggtcctc cacttcatcc cgttcatagg
      841 atccgcaccg aaagaacgcc gcggacggca tgtcagtcat gaataatccc ccttgcgcgg
      901 tttagtataa taatactcta ccatctgcgg ccctttttgc ctccggtccg aacggccgga
      961 aggctgagca gaatgacgga aactgaaagc gacttgaaag gaccggagag agaatggcct
     1021 atgcgtccca gcagtttttc ctccagaagc gggaggtggg gctgattgcc ccagtactgt
     1081 gcactctgcg gattcttggg gaggatgaca tcatggaagc cgtcaccttc cacaggcgca
     1141 taacggacga gatcggcgac gccggctttt tcgccgagga caacaccatc gtggagagca
     1201 tccggggaga aggggcggtg tccgggatct tcgccgaggg caggcttatc gctctccggg
     1261 ccgtctccta cgtgaaggag tacgtggacg acgccctgga ggatctcggg cttgaccagt
     1321 cggaggcaaa caacacggct gtgatggatt tctgcgtcac cgattcggct ttccggggaa
     1381 acagcatcca gtttttcacc tatctcttta tggaaaacat tctctacccg aacaggtatc
     1441 acctgcacac caccgtttcc ccgaagaaca tcttcagcct cacgaacata ctgaagtgcg
     1501 gcttccacgc catgcgcttc aagagcaagt acggcggcca ccccaggttc gtgctctaca
     1561 aaaacctccg gaagcccatg gccgtgcaga caaggggaca caaggagatc ctcctgagaa
     1621 actatgacta ccacccgaag gtgttcgccg acggtttcgt ggggtacaag gtgaaacaca
     1681 agtccaacgg aatggccctg ctctacggca aacccctcgt cgtcagggag gagccgaagg
     1741 cgtcatgaag gaccttcttt ccggagcggg ggaaattctc cgccgcctgg aagaagaagg
     1801 gggggaggcc gtcattgtgg gaggcatggt cagggacctg ctctgcggtt tccctccctg
     1861 cgacgccgac attgcatccg acgcgcccct gcccgtcctg gcttccctct ttccgtccgg
     1921 caggatcctc ggccccgagg ggatgcaggt tttcctcctt ccgttcgggg aggggcactg
     1981 cgaagttttc cccttccccc gggggggatt gagagatgat cttgccaggc gggatttcac
     2041 ggtgaacgcc ctcgctctcc gcagaacggg ggaagtggag ggaagccgga gagccctggc
     2101 cgacattgcc gcccgggtga tccggttcaa cgggcccggg gccgaaaggc tggcggaaga
     2161 tcctctccgg gcgctccgcc ttgcccggtt cgccgccgtc cttcccgggt tcgttcccga
     2221 cccgggggcg atcagggaat gccgggaggc ccgtccttcc ctcgaaagat gcgctcccga
     2281 aagggtgggg cgggagattc gcctcgggct ggaggggaat tcccggatat tcctggagac
     2341 ggtgaaggcg tgcggcctcc tggatgtcct gtttccggga atgccgtcga gggagttcga
     2401 cttctccagg ctctgccgta tccaggaagg gctggcccgg gaaggggcgc cgctcgaggt
     2461 ccgggccgca gctcttttcc cggtgcccgg ggggaggcct tcccggtccg acgacgggcc
     2521 gtcaagggcg gaagaaaccc ttctcgcctg gaaatggtcc gcccggtcag cctcggaggc
     2581 cgccgaactc gccaggcaga ggaggcttcc cctggaagcg cctgacccgg agaggacggc
     2641 agccctgctc gaggccaggg ggacgccgtt catggattcc ctgttccttc tctcccggca
     2701 gtactgcgcc ggagagcacc acttccgccg atggagcgaa aaccgtgccc tgtacgtttc
     2761 catggcggtc cgggcactcc ggaacgacgt gctcccgtcg ggggccgaga tcatgaaaaa
     2821 gttctccctt gccccgggcc ctctcatcgg agagctcgcc gacgcagtga aactccgcag
     2881 gctccacccc ggcctcggat cgaaagagga cgccttccgc tttctggaaa cccttctcag
     2941 gcaaaaccgc aatccctgat cttccgtccc ggacagaaga gtgatacaat tcagcaaaat
     3001 ggtcccccgg aagaattcct gccggaggac catttcagac ccgccccggg gagggattcc
     3061 catgaagaag gcagccccat ggttcgttct tctgtttttc cttgcggttc cgatggcagc
     3121 ccctccggga gaagcggagg ccgctcccgc aatcaccgaa acctacctcg cctcgtccgt
     3181 gaaggaagac ggggctccag tggggcgggt ctcagcctac gacccgtcgg cgaaggaatt
     3241 cgtcgccgtg gcggaggtca gggacgctca cg
//
LOCUS       DAOTHD010000125         3269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_34680_length_3269_cov_3.717175, whole genome shotgun sequence.
ACCESSION   DAOTHD010000125 DAOTHD010000000
VERSION     DAOTHD010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34680_length_3269_cov_3.717175"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="P5219_03145"
     CDS             <1..591
                     /locus_tag="P5219_03145"
                     /inference="COORDINATES: protein motif:HMM:NF019307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HRX25784.1"
                     /translation="KAAHAGGAPNEGRNALLAAASATLALAGIPPHKDGATRINVGVL
                     QAGSGRNVIPSRAVMKVETRGLTMELNSYVYDRAMEIVSGAASMYGVEMTMAKMGEAV
                     DALSDEDLAGIVRDRAATISGLERIAPPVSLGGSEDVSWMMRAVQKRGGKAVYFVLGT
                     DIAAGHHNEYFDIDEKVLDYGPALFAELALVLAAGK"
     gene            681..2261
                     /locus_tag="P5219_03150"
     CDS             681..2261
                     /locus_tag="P5219_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HRX25785.1"
                     /translation="MSEATAPKKRGLLMRFIKGVEIVGNKLPHPFWLFVILSGVILAL
                     SWWLGNAGMSVKYMAAGRGGAEAKETVVSVVNLLTFKSMRVVFTDLLKIYSSFPPLGL
                     IIVMTLGIGLLEQSGMISALMRKTILGAPSYLVTFALAIVGINANLASDAGIIFTPMI
                     GAAIFKALGRNPWIGITAGYAASQGGFTANFFIAGTDALLAGITESAAKGMNIPGPVH
                     PLMNWYLMVAATVVVAFVTTFVTEKITCKYLGDEGGTMDATELEKHRVTDVENRGLKW
                     SLYAGLAYILLIVALTFPEGSFFRADNGDILPRSPFLSSIAGILFFLFFFMGVAYGYG
                     AKVIKAGSDIPKMMEKGLKGGISFLVVVLPAAIFIHLFNASNLATILAVDGADWLKSM
                     NLTGSKLIIAFVLLTTTINLFISSGSAKWLILAPIFVPMLGMMGFSPALTQAAYRVGD
                     SCTNNLAPLSYYVPVMIGFYEQYKQDADQEVGIGTLISLQLPFSILYLILFTALLVFW
                     FTMGWPLGPGADLHIPMP"
     gene            2580..>3269
                     /locus_tag="P5219_03155"
     CDS             2580..>3269
                     /locus_tag="P5219_03155"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRX25786.1"
                     /translation="MKNVPADMEGFLSHQPWWRTRVPTEIIRSREGVGELLRRLEREN
                     RNPFFVVDSVLLNQPVFASLLGQKALYLFDASASEPKTGDVDTVVSMVKSASKAHDVV
                     VGIGGGATMDLAKAVGICLANPGPAQTYQGYGLDMKKGADIWVLPTLSGTGAEITPIA
                     VLRGPEKKLGINNPYTAPSVAVIDPGLTSGVRKYNRFFTLMDCYFHHYEIVNSRTSSE
                     DAVSDARDGLSL"
BASE COUNT          716 a          905 c          914 g          734 t
ORIGIN      
        1 aaagcggccc atgcaggcgg agcgccgaat gaaggccgaa acgccctgct cgcggcagcc
       61 tccgccaccc tggccctggc gggcattcct ccccacaagg acggggcgac caggatcaat
      121 gtaggcgttc ttcaggccgg gagcgggcgg aatgttattc cttcccgggc cgttatgaag
      181 gtggagaccc ggggtctgac catggagctc aactcctatg tgtatgacag ggcgatggaa
      241 atcgtatccg gagccgcctc catgtacggc gtggagatga ccatggcgaa aatgggagag
      301 gctgtggatg ccctgagcga cgaagatctt gccgggatcg tcagggacag ggctgcgacc
      361 atcagcggct tagagcgtat tgcccccccc gtcagcctcg gcggcagcga ggatgtttcc
      421 tggatgatga gggctgtcca gaaaaggggc ggcaaggcgg tatattttgt tctcggaacc
      481 gacatcgccg ccgggcacca caacgagtat tttgacatcg atgaaaaagt gctggactac
      541 ggtcccgcac ttttcgcgga actggccctt gtactggcgg ccggaaaatg atgccggaag
      601 gaggagggag agaagaaaaa aaaacaacga atggcggtga cagcaagact gcttcataat
      661 tttgcaaagg aggtattttc atgtcggaag caactgcacc caagaagcgg ggtttgctca
      721 tgcgcttcat caagggagtt gagatcgtag gaaacaagct cccccatcct ttctggctct
      781 tcgtcattct ttcgggtgtc atactggccc tgtcctggtg gctcggcaac gcgggcatgt
      841 cggtcaagta catggctgcc ggaaggggag gagccgaggc gaaagagacc gtcgtctcgg
      901 tagtgaacct ccttaccttc aagagcatgc gtgtggtgtt cactgacctt ctgaagattt
      961 actcaagctt cccccctctc ggcctgatca tcgtcatgac gctgggcata ggactcctgg
     1021 aacagtccgg aatgatctcc gccctcatgc ggaagaccat cctcggggca ccttcctatc
     1081 ttgtgacctt tgcccttgcc atcgtcggca ttaacgcgaa tcttgcgtcc gacgccggca
     1141 tcatcttcac gcccatgatc ggtgcggcca tattcaaggc cctggggcgc aacccgtgga
     1201 tagggatcac ggcaggatat gccgcttccc agggaggttt caccgcgaac ttcttcattg
     1261 cgggaacgga cgcgctcctt gcaggcatca ccgagtcggc cgccaaggga atgaacatcc
     1321 ccgggccggt acatcccctg atgaactggt acctgatggt cgcagccact gtggttgtcg
     1381 ctttcgttac gacgttcgtg acggagaaga tcacgtgcaa gtacctcggg gacgaaggcg
     1441 gcacaatgga tgccaccgag cttgaaaaac accgggtcac tgatgttgag aacaggggtc
     1501 tgaaatggtc tctgtacgca ggccttgcct atattcttct cattgtggct ctcactttcc
     1561 ccgagggatc cttcttcagg gctgacaacg gagatattct tccccggtct cccttccttt
     1621 ccagcatcgc cggcatcctc ttcttcctct tcttctttat gggggttgcc tacggctacg
     1681 gtgcaaaagt catcaaggca ggatcggata tccccaagat gatggaaaaa ggactgaagg
     1741 gcgggatcag cttcctcgtg gtcgttctgc ctgcggcgat cttcattcac ctcttcaacg
     1801 cgagcaacct ggcaaccatt cttgccgtgg acggagccga ctggctgaaa tccatgaatt
     1861 tgaccggaag caagcttatt attgccttcg tccttctgac gacgaccatc aacctgttta
     1921 tttccagcgg atcggcgaag tggctcatcc tcgcgccgat cttcgttccc atgctgggca
     1981 tgatgggatt ctctcccgca ctgacccagg cggcatacag ggtcggagac tcatgcacga
     2041 acaaccttgc ccctctttcg tattacgttc ccgtaatgat cggtttctat gagcagtaca
     2101 aacaggatgc ggaccaggaa gtgggaatcg ggacactcat ttccctccag cttcccttca
     2161 gcattctgta tcttatcctc tttacggcac tcctggtttt ctggttcacc atgggatggc
     2221 ctctcggtcc cggggcagac cttcatatcc ccatgccgta attagcccgg tcgttcgaaa
     2281 gcagagggaa ggacagcctt ccctctgctt ttttggtgcg cccggcatgg ttgttgtttt
     2341 cggtgagaac gtcctcagcg gtgaaggaaa aaagcggaaa caagacagga cggcacggtt
     2401 tttcccatct cgaatgaaga actctttcct gaaggactct ggaccggaga aaaaacttgg
     2461 agtacacttt tccccgtgtt tctccctctg ctttttccct ccaaaaccgg caggacatgg
     2521 ggaaggaaaa aacggtactg tggagagcaa tctttttcga ggtctggggt gaaacagaaa
     2581 tgaaaaacgt accggcggac atggaaggtt ttctttccca tcagccctgg tggcgtacca
     2641 gggtcccgac agaaataatc cgttcccgtg aaggagtggg ggagcttctt cgcaggctgg
     2701 aaagggaaaa caggaatccc ttcttcgtgg tggactccgt tctgctgaac caacctgtct
     2761 tcgcctccct tttgggacag aaagccctgt atcttttcga cgcctccgcg tcggaaccca
     2821 aaacaggaga tgtggatacc gtagtctcta tggtgaaaag cgcttcaaaa gcccatgatg
     2881 ttgtagtcgg catcggcggg ggagcgacca tggacctggc gaaggccgtc ggcatatgtc
     2941 tcgccaatcc aggtcccgcc caaacatacc agggatatgg actggatatg aaaaaagggg
     3001 cggatatctg ggttcttcct acactcagcg gaacaggggc agaaattacg cctatcgcag
     3061 tgctccgcgg gcctgaaaag aagctgggca tcaacaatcc ctatacagct ccctccgttg
     3121 cggtcatcga cccgggactc acttcgggag tccggaaata caaccgtttt tttaccctga
     3181 tggactgcta cttccatcac tacgagatcg taaacagccg aaccagttcg gaagatgccg
     3241 tttccgacgc ccgggacggg ctcagcctg
//
LOCUS       DAOTHD010000126         3240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_35096_length_3240_cov_2.432025, whole genome shotgun sequence.
ACCESSION   DAOTHD010000126 DAOTHD010000000
VERSION     DAOTHD010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35096_length_3240_cov_2.432025"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="P5219_03160"
     CDS             <1..430
                     /locus_tag="P5219_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HRX25787.1"
                     /translation="VMERTNARSLTPDLFREIPGLAVADASFISLKLLIPPISLILSP
                     EGQAILLVKPQFEVGKGRVGKGGVVRAKEDHVSVLKDILAFCEENGNFRPEGLTFSPI
                     TGPMGNIEYLLHLVRAAGPGRLISPEETVEEAHHFFRKDG"
     gene            434..1315
                     /locus_tag="P5219_03165"
     CDS             434..1315
                     /locus_tag="P5219_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HRX25788.1"
                     /translation="MADPRIGMLVNTRKPGALAMAGSLIEWGKASGVSFLFPPHEASV
                     LGIQGVDDEEWKRAVSIAVVIGGDGTFLRAARYVLGAPISLYGINLGHLGFLASGKAE
                     EAENDLEAILRGQYSLLSHRVLEGKICRDGKNIHTLFALNDLVLAKGAMARVMHIEVS
                     MGDKVLNTLPADGIIVSTPTGSTAYALSAGGPIVPPHIPCMIIVPICAHTLYTRPVLA
                     GENDVITLTPKGNHRDLLLTQDGQLGYEILPGDSIRISLNGEKAVSVIALPTRNYFDL
                     LQEKLQWGRGIVTFEKE"
     gene            1584..3008
                     /locus_tag="P5219_03170"
     CDS             1584..3008
                     /locus_tag="P5219_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RecN"
                     /protein_id="HRX25789.1"
                     /translation="MLFARRTFSRNGRNRTYFQDRAVPLSTFSTVMNEEMRIQSQFAQ
                     IELLDPKRQMEILDFCGGVEVSNLKEALSGTFAGAVECDRSLRAAKSREQELKTRFRD
                     GEAILTAAKGLKLAPGCENEWESQIRTLSLRVDRMKKARDNLLRMTGGAAGQGLLDLL
                     ESSGLELLRCLENDEGGLPKLFNEGLERLQAFSREAARMTAGQSVEEMEREQDLLEKR
                     GGLLRKLKRASGTTSVEDFLEWCDEAKRASEWIREQERNLAELTEKGRGFRKEAAGLA
                     AELRSLRAATASRLERDVNRHLADLAMEDCRFSVRLTELEKIRSTGADDVFFTLSTGG
                     KGETPVNKTASGGELSRILLALQLSLPESSLPPTLVFDEVEAGLGGRAAVLAGYKLQE
                     LSRRCQVILVTHEATIAALAEHHYLVRKNGESVEAVKLGKEERAAEIARMLSGDAGLP
                     EAMEHAKKLLSGRPEYLISADSKN"
     gene            3120..>3240
                     /locus_tag="P5219_03175"
     CDS             3120..>3240
                     /locus_tag="P5219_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL21 family ribosomal protein"
                     /protein_id="HRX25790.1"
                     /translation="MYAIVETGGKQYRVQEGDFLKVESLGVDEGETVLLDKVLL"
BASE COUNT          759 a          849 c          967 g          665 t
ORIGIN      
        1 ggtcatggaa aggaccaacg cccggtcact cactccggat cttttccggg aaatccccgg
       61 tctcgcagta gccgatgcgt cctttatttc cctgaaactc ctgattcccc cgatcagcct
      121 gattctctcc ccggaggggc aggccatact gctggtgaaa ccccagttcg aagtgggaaa
      181 aggccgggtc ggcaaaggcg gagtggtcag agcgaaggaa gatcatgtat cggttctgaa
      241 ggatattctc gccttctgcg aagagaacgg taattttcgc ccggagggct tgactttttc
      301 tccgattaca ggtcccatgg ggaatattga gtatcttctt cacctggtca gggcagcagg
      361 acccggcaga ttgatttcgc cggaggaaac agtagaagaa gcccatcact ttttccggaa
      421 ggatggatag gcaatggctg atccccgcat cggaatgctt gtcaacacaa gaaaaccggg
      481 agcgctggca atggccggat ccctcatcga atggggaaag gcgtcgggcg tatcttttct
      541 gttcccaccc cacgaggcct ctgtcctggg aatacaggga gttgacgacg aggagtggaa
      601 aagggccgtt tccatagcgg tcgtcatcgg aggcgatggt accttcctca gggcggccag
      661 atacgtgctt ggtgccccca tctctctcta cggcatcaac ctgggccatc tcggtttcct
      721 cgcgtccggc aaggccgagg aggcggaaaa tgacctggag gcaattctga gaggccagta
      781 ttccctgctg tctcaccggg ttcttgaagg gaaaatctgc agggacggaa aaaacatcca
      841 taccctgttc gccctgaatg acctggtcct cgccaaaggc gccatggcca gggtgatgca
      901 tatcgaggtg agcatgggcg acaaggtgct caatacgctg ccggcggacg gcatcatcgt
      961 ttccactccc acggggtcca ctgcctatgc cctgtctgcg ggaggaccga ttgttcctcc
     1021 ccacatcccc tgcatgatca tcgttcccat ctgtgcccac acgctctata cccgtccggt
     1081 gcttgccgga gagaatgatg tcataaccct gaccccgaag ggaaaccaca gggatttgct
     1141 tctcacccag gatggacagc tcggatatga aattcttccc ggggacagca tacgaatctc
     1201 cctgaacggt gagaaggccg tcagcgtgat tgccctgccc acgagaaact acttcgatct
     1261 tctccaagag aaactccagt ggggaagggg aattgtcacc ttcgaaaagg agtgaatccc
     1321 ccatgatcga ggaagtgacc attcggaacg ttgggggagt ggcttcagca gccctccgtc
     1381 ttgaaaaagg actgaccgtc ataacgggtg agagcggagc ggggtagagc agccttgtcc
     1441 gttctctcgt acttctgggc ggcaaaagga gccagtcggc attcctccgt tccggcgagg
     1501 aagaaggatc cgtcgaggcg gtgctttcag gagtttcagg tgacagggca ggcagtgcat
     1561 acgccgggac ggatgacggg ttcgtgcttt ttgccaggag aaccttttcg agaaacggac
     1621 gaaaccggac gtactttcag gacagggccg ttcccctttc cactttttcc acggtgatga
     1681 acgaagaaat gcgcatccag agccagtttg cccagatcga gctgcttgat ccgaaacggc
     1741 agatggagat tctcgacttc tgcgggggag ttgaggtatc gaacctgaaa gaggctttgt
     1801 ccggaacctt tgccggagct gtggaatgcg accgctccct ccgggctgcg aagagccgcg
     1861 aacaggaact gaaaacccgt ttccgggacg gtgaggctat cctcacggcg gcaaaggggc
     1921 tgaaactggc cccggggtgc gaaaatgaat gggaatcgca gatccggacc ctttcactca
     1981 gggtcgaccg tatgaaaaaa gccagggaca atcttctccg gatgacggga ggagctgcgg
     2041 gccaggggtt gcttgatctg ctggaatcaa gcggtcttga actgctgcgg tgccttgaaa
     2101 acgatgaagg cggcctccca aaactcttca acgaaggtct ggagaggctc caggctttct
     2161 caagggaagc ggcccggatg acggcagggc agtcagttga agaaatggaa agagagcagg
     2221 atctccttga gaaaagggga ggtcttctga gaaagctgaa aagagcctcc gggacaacct
     2281 ccgtggagga ttttctggaa tggtgcgatg aagcgaaaag agcttccgaa tggattcggg
     2341 aacaggaacg gaatttggct gagctgaccg aaaaaggccg ggggttccgg aaagaagctg
     2401 ccgggcttgc ggccgaattg cgctcccttc gggccgctac tgcctcaaga ctggagcggg
     2461 atgtcaaccg tcatctcgct gacttggcca tggaggactg ccggttttcc gttcgtctca
     2521 ctgaactgga aaaaatcagg agcaccggag cggatgatgt ttttttcacc ctgtccacag
     2581 gcggaaaagg ggaaactccg gtgaacaaaa ccgcatcggg cggtgaactg agccggattc
     2641 tccttgctct ccaactgtct ctcccggaat catccctccc gccgactctt gtattcgatg
     2701 aagttgaggc aggccttggc ggtcgcgcag ccgttcttgc cggatacaag ctccaggaac
     2761 tttcccgcag atgccaggtc attctcgtca ctcacgaggc aaccatagcg gcactggctg
     2821 aacatcacta cctggtgagg aaaaacgggg aatcggtgga ggccgtcaag ctaggaaaag
     2881 aggagcgggc cgcggaaatt gccaggatgc tttccgggga tgccggtctt cccgaggcaa
     2941 tggaacatgc gaaaaaactt ctttccggaa ggcctgaata cctgatttcc gctgattcaa
     3001 aaaattgaca ttcactgaag aacttcgtat aatgctacgg ttcggtttat atatcgcacg
     3061 gggcaggtca taaacctgca cacacaggtg cctgaacccc cggcgggagg aggaaaagaa
     3121 tgtacgctat cgttgaaaca ggcggcaagc agtaccgtgt ccaggaagga gacttcctga
     3181 aggttgagtc cctcggagtt gacgaagggg aaaccgttct cctcgataag gtactccttg
//
LOCUS       DAOTHD010000127         3236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_35156_length_3236_cov_3.340459, whole genome shotgun sequence.
ACCESSION   DAOTHD010000127 DAOTHD010000000
VERSION     DAOTHD010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35156_length_3236_cov_3.340459"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="P5219_03180"
     CDS             <1..965
                     /locus_tag="P5219_03180"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRX25791.1"
                     /translation="GWAVYRDVQPNPTDVMVMRGVEVFDGEGCDLILTLGGGSPMDCA
                     KAIGAVHANGGNVLDFVGVDKIPVPCPPVICVPTTAGSAADISQFSIIKNEKTGIKHA
                     IVSKSIVPDVALVDPDTTLTMDGFLTASTGMDALTHAFEAYVSRAHSPLTDVHALQSV
                     EMVHSFLLRAMGDLSSPEYRTGMMLASLHAGLAFSNASLGVVHALSHSLGGLLDLPHG
                     ECNSLLLRHGVEANYGAAPERFAVLLERFGGCAETPDVLSELLARLDTLRDQAGLPGR
                     LRDLGLKEDLLPEIAAAAWEDPCMITNPRPLSPAGLEEILRNAY"
     gene            955..2043
                     /locus_tag="P5219_03185"
     CDS             955..2043
                     /locus_tag="P5219_03185"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRX25792.1"
                     /translation="MPTDRRTTEQDDQLLALMGLGARSGKKSHYPELKRRLAELERVQ
                     ALLDRAQDAIFVVSLPDETVEYRNEAALALRGGFGRGELRPLEEFLKGPDGRGLTIRS
                     CFPAFPDGEERMILPLVTEKGVRRFEASFLAAETDGRLSGVLILRDQEDRIRAETRLA
                     ESLRQVEQARMRTVHLTATLVEIKDSYTGKNQRQAAGLAHAIGTGLGMTGDPLENLVT
                     ATLLHDVGMMAIPAEILCIPGPLRPVDRRLMEEHASIGCDILKKQGFPPEIYLTVLHH
                     HERLDGSGYPEGLKGEDIPFGARIAGIADVTEALLSHRPYRPAWPLEKVLEELSGKKG
                     VLYDPDGAEICIRLLEEGYTLDDQWFYK"
     gene            complement(2085..2393)
                     /locus_tag="P5219_03190"
     CDS             complement(2085..2393)
                     /locus_tag="P5219_03190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRX25793.1"
                     /translation="MKVDITKSGTGAQVRLGGSMYVEDSASVREQLIGLIEDGMVHLT
                     VDLSDLDYVDSSGLGVLISIHKRCLQKGGKMVITGLRGMVEELFKLTRLDLVFNVSRQ
                     "
     gene            complement(2439..>3236)
                     /locus_tag="P5219_03195"
     CDS             complement(2439..>3236)
                     /locus_tag="P5219_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRX25794.1"
                     /translation="AIWMSSLIQIAQSLAASADKNSAEMSGAAEEGRKTVAQTVERME
                     NIRLSVEETEQLLSQLDTFSARIGVVGDTITGLADQTNLLALNAAIEAARAGEAGRGF
                     AVVAEEVRKLAEQSQQGAREVAELVGKILDGTRSAVASMQKSRQGVEEGVSIAHVAGE
                     SLERIGKAISSSVEDIRKIISTTDEEVAKSDKVISLIDTTASVMELTDDHVQTLAASM
                     EETAAAMETVATSAQEVSETSEDMRRMTERFKVDKDGGVLSNKPAVV"
BASE COUNT          597 a         1097 c          951 g          591 t
ORIGIN      
        1 tgggctgggc cgtctaccgc gacgttcagc cgaaccccac cgacgtcatg gtcatgcggg
       61 gcgttgaggt ctttgacggc gaaggctgcg acctcatcct caccctcggc ggaggaagtc
      121 ccatggactg cgccaaggcc atcggagcgg tccatgccaa cggagggaac gttctggatt
      181 tcgtgggggt cgataaaatc cccgttccct gccctcccgt gatctgcgta cccaccaccg
      241 ccggaagcgc cgcggacatc tcccagtttt ccattatcaa aaacgaaaaa acagggatca
      301 agcacgccat cgtcagcaaa tccatcgttc ccgacgtggc cctggtggac ccggacacga
      361 ccctcaccat ggacggcttc ctcacagcgt ccacgggaat ggacgccctc acccacgcct
      421 tcgaggccta cgtctcccgg gcccattcgc cccttacaga cgtccacgcc ctccagtccg
      481 tggagatggt acactccttc cttctccggg ccatgggcga cctttcaagc ccggaatacc
      541 ggaccgggat gatgctggcg tccctccatg cgggcctcgc cttctccaat gcgagcctcg
      601 gcgtggtcca cgcactctct cacagcctcg gcgggctcct cgacctcccc cacggggaat
      661 gcaactccct gctcctccgc cacggcgtgg aggccaacta cggcgccgcc cccgaacggt
      721 tcgccgtcct cctcgaacgg ttcggcgggt gtgctgaaac tcccgatgtc ctctccgaac
      781 tgctcgcccg ccttgacacc ctgcgggatc aggccggcct tccgggaaga ctcagggatc
      841 tcggactgaa ggaggatctt cttcccgaaa tagcggcggc ggcatgggaa gacccctgca
      901 tgatcaccaa cccccggcct ctctccccgg ccggccttga ggaaatcctc cgaaatgcct
      961 actgaccggc ggacgacaga gcaggacgac cagctcctgg ccctcatggg gctgggagcc
     1021 cggtcgggga agaaaagcca ctacccggag ctgaaacggc gcctcgccga gcttgaacgg
     1081 gtccaggccc tgctggacag ggcccaggac gccatattcg tggtttccct ccccgatgaa
     1141 acagtggaat acaggaacga agcagccctg gccctccgcg gcggcttcgg ccggggtgaa
     1201 ctccgccccc tggaggaatt cctgaaaggc cccgacggga gaggactcac catcaggtca
     1261 tgttttcccg cttttccgga cggagaagag cggatgatcc tccccctggt gaccgagaag
     1321 ggcgtccgcc ggttcgaggc ctcgttcctg gcggcggaga ccgatggccg cctctccggg
     1381 gtgctcatcc tcagggacca ggaggacagg atccgtgcag agacgaggct tgccgaatcc
     1441 cttcggcagg tggaacaggc ccggatgcgg accgtccatc tcaccgcaac cctggtggag
     1501 atcaaggact cctacacggg gaagaaccag cgccaggcgg caggcctcgc ccacgccatc
     1561 ggcaccgggc tcggaatgac gggcgacccc ctggaaaacc tggtgacggc cactctcctc
     1621 cacgacgtgg ggatgatggc cattcccgcc gaaatactct gcattccggg acctctccgg
     1681 ccggtggacc gccgtctcat ggaggagcac gcctccatcg gctgcgatat cctgaagaaa
     1741 caggggttcc cgccggaaat ttacctgacg gtgctccacc accatgaacg cctggacggc
     1801 agcggctacc cggagggcct gaagggagag gacatccctt tcggggcccg gatcgccggc
     1861 atcgccgacg taacggaggc cctgctgagc catcgcccat accggcccgc ctggcccctg
     1921 gaaaaggtgc tggaggaact ctcgggaaaa aagggggttc tttacgatcc cgatggggct
     1981 gaaatctgca taaggctgct tgaggaaggg tataccctcg acgaccagtg gttttacaaa
     2041 taacggggaa aaaaccgggg gccctgaagg gcccccggaa cgcgttactg gcgggacacg
     2101 ttgaacacga gatccagccg ggtaagcttg aacaactcct ccaccatgcc gcgaaggccg
     2161 gtgatgacca tttttccgcc cttctggagg catctcttgt gaatggaaat caggactcca
     2221 agcccggaac tgtccacgta gtcaagatcc gaaaggtcga cggtcagatg caccattcca
     2281 tcctcaatga gcccgataag ctgctcccgg accgacgcgg aatcctcgac gtacatgctg
     2341 ccgcccaggc gcacctgcgc cccggtgccg cttttggtga tatccacttt catggcgaat
     2401 cctccttctc cacggtcaaa cttcggaaaa caggccaatt atacgacagc gggcttattt
     2461 gacaaaaccc cgccgtcctt gtccaccttg aagcgctccg tcatgcgccg catgtcctct
     2521 gaggtctcgc tcacttcctg ggcgctggtg gccacggtct ccatggctgc cgccgtctct
     2581 tccatggagg cggcaagggt ctgcacgtga tcgtcggtaa gttccatgac gctggcggtg
     2641 gtgtcgatga gggagatcac cttgtcggac ttggccacct cctcgtcggt ggtggaaatg
     2701 atcttcctga tatcctccac ggaactgctg atggccttgc cgatacgctc aagggattct
     2761 cccgccacgt gggcgatgga gaccccctct tcgaccccct gccggctttt ctgcatggac
     2821 gccacagcgg acctggtccc gtccaggatc tttcccacca gttccgccac ttcccgggct
     2881 ccctgctggg actgctcagc cagcttccgg acttcttccg ccaccacggc gaatccccgt
     2941 ccggcctcgc ccgccctggc cgcctcgatg gcggcgttga gggcaagaag gttggtctgg
     3001 tcggcgaggc cggtgatggt gtcgcccacc acgccgatcc tggcggaaaa ggtgtcgagc
     3061 tgggaaagga gctgttccgt ctcttccacg gaaagccgga tgttctccat gcgctccacg
     3121 gtctgggcga cggttttccg cccctcctcc gccgccccgg acatctcggc ggagtttttg
     3181 tcggcgcttg ccgcgaggct ctgggctatc tggatgagac tgctcatcca gatagc
//
LOCUS       DAOTHD010000128         3235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_35171_length_3235_cov_3.038679, whole genome shotgun sequence.
ACCESSION   DAOTHD010000128 DAOTHD010000000
VERSION     DAOTHD010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35171_length_3235_cov_3.038679"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1592)
                     /gene="selB"
                     /locus_tag="P5219_03200"
     CDS             complement(<1..1592)
                     /gene="selB"
                     /locus_tag="P5219_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HRX25795.1"
                     /translation="MDRREISLVLGTAGHIDHGKTTLVKAITGVDCDRLSEEKKRGIT
                     IELGFAPLVLDDGRIVSIVDVPGHERFIRQMVAGASGIDGVLLVVAADEGVMPQTREH
                     LEILKLLGVKDGLVAVTKADSVEEDFLELVLEDVKDFLAGSFLEGKPVIPVSAISGKN
                     LPLLREELGALVDRIRPRPRKGPFFLPIDRTFPVAGFGAVVTGTAYRGEISVGMEAAA
                     LPSGGEGRVRSVQVHGAGVQTAWAGQRVAVSISGISSEELSRGDVLCAKGIYRPTRCF
                     ECELSLLETAGEPARHWQRVRLHIGTSDVVARIALLDNPLLQPGETAPAQIVAEEDIV
                     CLVDQRFVLRRYSPLETIAGGRVLFPYAAKPKGRKAKNSCISRVRDLASVHGPEERLL
                     ALVRSFSILELSDGALYIQESTGETARLAEKLGKREDTVFLQGEKKLLLSRESFLRLA
                     GDVEAFLGSYHDSHPSQKGAPPDVTVLSALKSFDSRTAKAFIEALVSGGSVVVTEEGL
                     LRMKDFQPRDDKAFERHSAGILS"
     gene            complement(1597..3000)
                     /gene="selA"
                     /locus_tag="P5219_03205"
     CDS             complement(1597..3000)
                     /gene="selA"
                     /locus_tag="P5219_03205"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HRX25796.1"
                     /translation="MKAQTAEKNRLRSLPAMDCLLGMPDMDPFLENLGREAIKTVLGE
                     AMDSLRKKILAGEDVEPSAESVLKLALPVLAARSGGSLRPVINATGVVIHTNLGRSCL
                     APEAGKAVLAAAERYSTLEYDLSEGKRGHRSDHVEWILREITGAEAGIVVNNNAGAVL
                     LMLAGLCSGKEVIVSRGELVEIGGSFRIPDIMAFSGARLVEVGSTNRTHLKDYGNAIT
                     ENTAALLKVHPSNFRMEGFTSAVPREDLAALAKERGLLFLEDLGSGTLIDMAPFGLKG
                     EPTVSSCLKAGVDLVTFSGDKMLGGPQIGGLAGKKEIVDRLRIYPLLRALRVDKMTLA
                     AFEATLRLYLSGKHLRIPTLAMLKETGDSLRRKARRLSDRLKKILPADTVSLVEADDA
                     VGGGAFPAERLPGWAVGIVMDSLGSAGSVLSLLRSLPVPVIAGARDNMVLLHVRTLLP
                     GDEERICDAFRAVASGR"
BASE COUNT          676 a          946 c          894 g          719 t
ORIGIN      
        1 gaaaggatcc cggcggaatg cctttcaaag gctttgtcat ccctcggctg aaaatctttc
       61 atccggagaa ggccttcttc ggtgacaact acggaaccgc cggacacgag ggcctctata
      121 aaagcctttg cagtcctgga atcgaaggac ttcagggctg aaagaaccgt cacgtcggga
      181 ggagcgccct tctgcgaggg gtgggaatcg tggtatgagc cgagaaaggc ttcaacgtcc
      241 cccgcaagcc gaagaaagct ttccctggaa agaagcagct ttttttcacc ctggagaaag
      301 acggtgtctt ccctttttcc gagtttctct gccaggcggg ctgtttctcc cgtggattcc
      361 tggatataaa gagctccgtc ggaaagttcc agtatggaaa aagacctcac cagggcaagg
      421 agccgttcct ccgggccgtg cacagaggca agatccctta cccttgagat gcaggagttt
      481 tttgctttcc ttcccttcgg tttggcggca tagggaaaga ggacccttcc tcccgcgatg
      541 gtctcgagag ggctgtatct ccgcaggaca aaacgctggt ccacgaggca gacgatatct
      601 tcttccgcaa cgatctgggc tggagcagtt tctcccggct ggagcagggg attgtcgaga
      661 agggcaatcc gggccaccac atcggaggtg ccgatgtgga gccggaccct ctgccagtgg
      721 cgagcaggct ctccggcggt ctcgagcaga ctgagctcgc actcgaaaca ccgggtgggc
      781 ctgtagatcc ccttcgcaca gaggacgtcc cctcgtgaaa gctcttccga tgaaataccg
      841 gaaatgctta cggcgaccct ctggcctgcc catgcggtct ggactccggc accgtggacc
      901 tggacactgc ggaccctgcc ttcacctcct gaggggagag ccgctgcttc catgccgacg
      961 gatatctccc ccctgtaggc cgtcccggtg acaacggctc cgaaaccggc cacggggaac
     1021 gtcctgtcaa tggggaggaa aaacgggcct ttccgtggac gaggacgtat tctgtccacg
     1081 agagccccga gttcttccct gagaaggggg aggttctttc ctgaaattgc ggaaacggga
     1141 attaccggct tcccctccag gaaggagccg gcaaggaagt ccttgacgtc ttcaagaacg
     1201 agttcaagga aatcctcctc gaccgagtcc gctttcgtaa cagccacgag cccgtccttc
     1261 acgccgagga gcttcaggat ttcaaggtgt tcccgcgtct ggggcataac tccctcgtcg
     1321 gcggccacca caagaagcac tccgtctatt cctgatgctc cggccaccat ctggcggatg
     1381 aagcgttcat gaccggggac atccacaatg ctgacaatcc gtccgtcatc gagtacgagg
     1441 ggggcgaatc cgagttcgat ggttattccg cgcttttttt cctcactgag gcggtcacag
     1501 tcaacacccg ttatggcttt tacgagagtc gtcttcccat ggtctatgtg tcctgcggtt
     1561 ccgagcacaa gggaaatttc ccggcggtcc acggtgtcac ctcccggaag cgactgccct
     1621 gaaggcatcg cagattcttt cttcgtcccc cggaaggaga gtccggacgt gaaggaggac
     1681 catattgtct cttgctcctg caataacagg aacaggaagg gatctgagca gtgagagaac
     1741 cgatccggcg ctccccaggg agtccatgac aattcctaca gcccatcccg gaagccgttc
     1801 cgcgggaaaa gctcctccgc caactgcgtc gtcggcctca accagggata cggtatcggc
     1861 aggaagtatt tttttcagcc tgtccgacag tctccgggcc ttccttcgga gggaatcacc
     1921 ggtctccttc agcatggcga gcgtcggaat cctgagatgc ttgccggaaa gataaagacg
     1981 aagggttgcc tcgaaagcgg caagggtcat cttgtccact cggagtgccc tgagaagggg
     2041 ataaatccgc agtctgtcca caatctcctt ttttcccgcc agaccgccga tctgggggcc
     2101 gccgagcatt ttgtctcccg agaacgtcac gaggtccaca ccagctttga ggcacgacga
     2161 gaccgtgggc tcccccttca ggccgaaggg ggccatgtcg atgagggtcc cgctcccgag
     2221 gtcttccagg aaaaggagtc ccctttcttt tgcaagtgcg gcgagatcct cccgcggaac
     2281 ggcggaggta aatccctcca tccggaaatt cgacgggtgg accttcagaa gggccgccgt
     2341 gttttccgtt atcgcgtttc cgtaatcctt caggtgggtt ctgttcgttg accccacctc
     2401 cacgaggcgg gctcccgaaa aagccattat gtcgggaatg cggaaggatc ctccgatttc
     2461 cacgagttcc ccccttgaga cgatcacttc tttcccggag cacagtcctg ccagcataag
     2521 aagaaccgca ccggcattgt tgttcaccac aatcccggct tctgccccgg tgatttccct
     2581 caggatccat tcaacgtggt cgcttctgtg tccccgcttg ccttcggaaa gatcgtattc
     2641 gagggtgctg tacctttcag ccgcggcgag aaccgctttg ccggcctcgg gagccaggca
     2701 tgatcttccg aggttcgtgt ggatcactac ccccgtggcg tttatcaccg gcctgagact
     2761 tcctccggac ctggcggcaa ggacaggaag agccagtttc agaactgatt cagctgaagg
     2821 ttccacatct tcgccggcga gtatcttttt tcgaagagaa tccatggcct cgccgaggac
     2881 tgtctttatc gcttccctgc ccaggttttc cagaaaggga tccatgtcgg gcatgcccag
     2941 gaggcagtcc atggccggga gcgagcgaag tctgtttttt tctgcagtct gagccttcat
     3001 ggaagaaaca tcacctttct ccgggaaaaa aatgttgacc cggcgtcaaa aaaagccggg
     3061 aagcggcaaa atcctttgct cccctccata ttctacccta ctcgccttta gcatagggcg
     3121 gaagcttgct attacttggt ttcgaatacc ctcacatttt aaggttcgag gtatttgtac
     3181 gatcaacttg tattacttct gactttttga ccaatgtctt ttcgaaataa aaagg
//
LOCUS       DAOTHD010000129         3233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_35221_length_3233_cov_2.323474, whole genome shotgun sequence.
ACCESSION   DAOTHD010000129 DAOTHD010000000
VERSION     DAOTHD010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35221_length_3233_cov_2.323474"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="P5219_03210"
     CDS             <1..454
                     /locus_tag="P5219_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRX25797.1"
                     /translation="LMLAKTGGAIVFGVINAFIPVIMAVFFADVSGIFWTVFLAAVFF
                     IAVASTFLGLFIAVSVSEVFEAQTFSNFFRFPMIFLCGLFFPISRLPWLLRPISFCLP
                     LTYGADLLHGAIGGGNTMPLLMDLGLLVLFCAALFAASLWNIRRKWIA"
     gene            522..1448
                     /locus_tag="P5219_03215"
     CDS             522..1448
                     /locus_tag="P5219_03215"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRX25798.1"
                     /translation="MSDHKCVYGPVPSRRLGESLGISPIPKKTCNYSCIYCQLGRTDR
                     MTNRRSEFFPLQEIVDEFRTALTCGASFDAVTLVGEGEPTLYSRIGDLITEMKKYTEK
                     PVAVITNGALLYEKSLREELSHADIVLPSLDACDERTFRTINRPWTGLTFEKCFRGLA
                     GFSREYTGELWIETMVIDGINDDDESLHRMKDLLKEVRYSRLYINTPVRPPAEENVRP
                     VPPETMDRAVRILGGISIDQLVSVGFFSSEKDDYNAVLGIIRRHPMNQFEIGTFLKTR
                     GNAEAEKVLSRLNEDPAVQGVEYKGFVTYRLR"
     gene            complement(1589..2356)
                     /locus_tag="P5219_03220"
     CDS             complement(1589..2356)
                     /locus_tag="P5219_03220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRX25799.1"
                     /translation="MTEKFSRFSLEGERALITGGGTGIGFGIAESMVRAGAHVVLAGR
                     REELLRAAAEKLGDRAGYIAGDVTDTRGAEEFARTVEERFGPVSILVNNAGIHHKQPF
                     LETAFEDFDKVLRTHVSGSFVMSKAFAPSMAAGKHGHILFIASMTTFIGMPQVIGYTA
                     AKSAVGGMVRALTAELAPLGVRVNAIAPGWINSEMMHRAVNSDPERKAKILGRTPMGR
                     FGDPEDIGDAAVYLSSPAASFVTGVILPVDGGAVTGF"
     gene            complement(2397..3059)
                     /locus_tag="P5219_03225"
     CDS             complement(2397..3059)
                     /locus_tag="P5219_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HRX25800.1"
                     /translation="MQWSSDRELFALMKEKLYTPVVGDILDAMGYYHQFLPQAIRPLR
                     DDMKLAGRAMTVLMIDVFGVQKKPFGLLTEALDQLEEGEVYLASGGGRRCAYWGELLT
                     AASRTRGAVGAVVDGWHRDTPQVLEQNWPVFSMGCYAQDSSVRTQVADYRCRLEVGNV
                     TVLPGDIVFGDVDGVLVIPSKAADEAVEKALEKAAGEKTVRKAIEAGMSTTKAFETYG
                     IL"
BASE COUNT          608 a         1078 c          888 g          659 t
ORIGIN      
        1 cctcatgctc gccaaaacgg gaggcgccat cgtgttcggg gtgatcaacg ccttcatccc
       61 ggtgatcatg gctgttttct ttgccgatgt ctccgggatt ttctggaccg tttttctcgc
      121 cgccgttttc ttcattgccg tggcgtccac tttcctggga ctgttcatcg ccgtctccgt
      181 cagcgaggtc ttcgaggccc agacgttctc aaactttttc cggtttccca tgatcttcct
      241 gtgcggtctt ttcttcccga tcagcaggct cccgtggctc ctcaggccca tctccttctg
      301 ccttcccctg acatacgggg cagatctgct ccacggcgcc atagggggcg gcaacaccat
      361 gcctctcctg atggatctcg ggctgctggt cctcttctgc gccgcgcttt tcgcggcaag
      421 tctatggaat atacggagga agtggatagc ataacggcag cagtacgttt cgcatctttc
      481 aaagggaaca atgtgccgta caaacgaaag gaagcgaaaa aatgagcgac cacaaatgcg
      541 tctacggccc ggtcccttcg agaaggctcg gggaatctct cgggataagc cccatcccga
      601 agaaaacctg caattactcg tgcatttact gccagctcgg gagaactgac agaatgacca
      661 acaggaggtc cgaatttttc cccctgcagg aaattgtgga cgaattccgc accgccctga
      721 cctgcggcgc ctctttcgat gcggtaaccc tcgtggggga gggggaaccg actctttact
      781 cccgcatcgg ggacctcatc acggaaatga aaaagtacac ggaaaaaccg gtcgcagtga
      841 taaccaacgg ggcgctcctc tatgaaaaaa gcctccggga ggaactttcc catgcggata
      901 tcgtcctccc ttccctggac gcttgcgatg aacggacgtt ccggacaatc aacaggccct
      961 ggaccggtct gacctttgaa aaatgcttcc gcgggcttgc cggtttctcc cgtgagtata
     1021 ccggggagct gtggatcgag acgatggtca tcgatggaat aaacgacgac gacgaaagcc
     1081 tgcacaggat gaaggatctc ctcaaagaag tcaggtacag ccgcctctat atcaacaccc
     1141 ccgtccggcc ccctgcggag gaaaacgtcc gccccgttcc gccggagacg atggaccggg
     1201 ccgtcagaat cctcggcggc atttccatag accagcttgt ctcagtcggc ttcttcagca
     1261 gcgaaaagga cgactacaat gccgtactcg gcataatccg ccgccatccc atgaaccagt
     1321 tcgagatcgg aacctttctc aaaacccgtg gaaacgcaga agctgaaaag gtcctctcca
     1381 gactcaacga ggatcccgcc gtccagggag tggaatacaa ggggttcgta acctacaggc
     1441 tcaggtgagc ggatggccgt cgctcatccc cggaacgtct gcgctccggg gaaacagtgt
     1501 cctttctgca gccggccgga aatcggggaa aaacagggga gccctttcgg gctccccttc
     1561 actgttcctt ttgacggcac aggtcccgtc agaagcccgt caccgcgccg ccgtccacgg
     1621 ggaggatcac cccggtgacg aaggaggccg cggggctgct gaggtagacc gccgcgtcgc
     1681 cgatgtcctc cgggtcgccg aaacggccca tgggggtcct cccgaggatc ttcgccttcc
     1741 gctccgggtc gctgttcact gccctgtgca tcatctccga gttgatccac cccggggcga
     1801 tggcgttcac ccgcaccccc agaggggcca gttccgccgt cagggcccgg accatgcctc
     1861 ccacggcgct cttcgccgcc gtgtagccga tgacctgggg catgccgatg aaggtggtca
     1921 tggaggcgat gaagaggata tgtccgtgct tcccggcagc catggacggc gcgaaggctt
     1981 tggacatgac gaagcttccg ctcacatggg tccgcaggac cttgtcgaag tcctcgaagg
     2041 ccgtctccag aaagggctgc ttgtggtgga ttccggcgtt gttcaccagt atggacacgg
     2101 ggccgaaccg ctcctccacc gtccgggcga actcctcggc accccgggta tccgtcacgt
     2161 caccggcaat gtacccggcc ctgtcgccca gcttttcagc agcagcccga agcagttctt
     2221 cccggcgccc ggcaagcact acatgggcac cggccctgac catggactca gctatcccga
     2281 agccgatacc cgtacctccg ccggtgatga gggcccgttc tccttccagg gagaaccggc
     2341 tgaacttttc cgtcattgtc atcccttctc tcttctcaat gccctgccgt cgatcctcag
     2401 aggatcccgt aggtctcgaa ggccttcgtg gtgctcattc cggcctcgat ggccttccgg
     2461 accgtctttt ctcccgccgc cttctccagg gccttctcca ccgcttcgtc agcggccttc
     2521 gagggaatga ccagaacgcc gtccacgtcg ccgaagacta tgtccccggg aagaacggtc
     2581 acgttcccca cctcgagcct gcaccggtag tccgccacct gggtccgcac cgaagaatcc
     2641 tgggcgtagc accccatgct gaacaccggc cagttctgct ccagtacctg gggcgtgtcc
     2701 ctgtgccagc cgtccaccac ggcacccacg gcgccccgcg tcctggatgc cgccgtgagc
     2761 agctcgcccc agtaggcgca tcgcctgccg cctcccgagg caaggtagac ctccccttcc
     2821 tcaagctggt ccagggcctc ggtgagcagg ccgaagggct ttttctgcac gccgaaaaca
     2881 tcgatcatga gcaccgtcat ggcccggccg gccagtttca tgtcatccct cagagggcgg
     2941 atcgcctggg gcagaaactg gtggtagtat cccatggcgt ccaggatgtc ccccaccaca
     3001 ggcgtatata gtttttcctt catcagggcg aaaagttccc tgtcgctgct ccactgcatg
     3061 gcacgacctc ccgttttcgt tcttccggcc cgctcccggg ctcagcctga aaatacggtc
     3121 tccctgcggg agacgcacat cctgtctatt ccgtcccagt ccacttccac gccgaggccc
     3181 ggcccctccg gcacctcgat gaagccgtcc tccgaaatct tcaggggttc ctt
//
LOCUS       DAOTHD010000130         3206 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_35610_length_3206_cov_2.909553, whole genome shotgun sequence.
ACCESSION   DAOTHD010000130 DAOTHD010000000
VERSION     DAOTHD010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35610_length_3206_cov_2.909553"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="P5219_03230"
     CDS             <1..222
                     /locus_tag="P5219_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HRX25801.1"
                     /translation="VHVNPGDIIVGDPDGVIVIPRQDAEDVLEAVKAFQQNDRRKLQA
                     AIDGTADRSWVMKQLEAKKCEFRDGAYRD"
     gene            292..1242
                     /locus_tag="P5219_03235"
     CDS             292..1242
                     /locus_tag="P5219_03235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRX25802.1"
                     /translation="MKRSVLAVLAAVCLVAVASPVAADDVFVRIGTSSVGGGFYLIGN
                     TIAQLGTEVEKSINFTAVTGGSIKNCINLGNKEVELGMVQSSTVNDAWNGTGSFKEPI
                     KSLRYVTAIYPMPAHILVNLDAEIKSIGDFKGKRVDYGSVGQGIEVNVREMMSVFGLK
                     DEDVRIERYGRSEVAEALKVGDSQAHIWTTNAPNAQVTDMIRSGKVGLIGIEPEKIQE
                     IVSKFPHYAPVVIPAGVYEGYDKDLPVVGAVGSLLTYEDMPEEVIYKITKMIHTNTKF
                     LQERLNYFNNLNLQFALAGMAVPLHPGAKKYYVEAGLIKE"
     gene            1323..>3206
                     /locus_tag="P5219_03240"
     CDS             1323..>3206
                     /locus_tag="P5219_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HRX25803.1"
                     /translation="MDTTVSGNQEKKTSSGKKRSFSGNLKLLVTVLTVALAVFQLYTA
                     FFGVLPAMQQRSLHLAFVLPLIFLLYPMWKTSPKNRPSLFDWMFAAGAALCTVYVYFM
                     YEDIANRAGMYTQYELYLGVVMIFLVFEAARRVLGYPLPIFCALFILFAYFGRSMPGP
                     FKHFGLSIPRIIEELYLTTDGLFGLVTGVSATYIYLFILFGAFLSSTGTSQFFNDLAM
                     ALTGHLKGGPAKIAVVSSAFMGTISGSTSANVATTGAFTIPLMKKIGYQPHFAGAVEA
                     AASTGGQIMPPVLGAAAFIIADSLGISYLKVLSAAVVPALLYFWGIWCCLSLEAAKLG
                     LQGLPKDALPRVKDVLVKSGYKSIPLFGIIYFLVQGYNPLYAGCWGIVLATLLSFVRK
                     EDRLNIRSFISTLEDGSKSALSVAIACVIVGVVIGMMGATGIALKVGDVILGFTKGNL
                     LLTLISTMILSMVLGMGMPTTASYVMASAVAAPALILLGGRPLDVHFFVFFFAVLSSV
                     TPPVCVGAYTAAGIAGSDPNRTAFTSIKLALSGFIVPFVFIYSPEIMLTNVESWPVTI
                     FAIITAIIGVFGLSVATEGYISAPVPLWGRVLALTGAVLLIFPGILTDATGFALLGMV
                     ILHN"
BASE COUNT          661 a          890 c          892 g          763 t
ORIGIN      
        1 gtgcacgtca accccgggga catcatcgtc ggagatcctg acggcgtcat cgtcattccg
       61 cggcaggacg cagaggacgt gctcgaagcg gtgaaggcct tccagcagaa cgaccgccgc
      121 aaactgcagg ctgccatcga cggaactgcc gacagaagct gggtcatgaa gcagcttgaa
      181 gcgaagaagt gtgaatttcg ggacggagcg taccgggact agaattgttc cgtagttttt
      241 gttcagcatc ataaacaccg ggaaaaccgg aaaaaaagga ggcagggaaa tatgaagcgt
      301 agtgtattgg ctgtactggc tgcagtgtgt ctggtggcgg ttgcgtctcc cgtggcggcg
      361 gatgatgttt tcgtccgcat cgggacgagc agcgtaggcg gagggttcta tctcatcggc
      421 aacacgattg cccagctggg gactgaggtc gagaagagca tcaatttcac cgcggtcacc
      481 ggcggatcca tcaagaactg catcaacctg ggcaacaagg aagtggagct cggcatggtg
      541 cagtcctcca ccgtgaacga cgcctggaac ggcaccggtt ccttcaagga gcccatcaag
      601 tctctccgct atgtgacggc catctatccc atgcccgccc atatcctggt gaaccttgac
      661 gccgaaatca agagcatagg cgatttcaag ggcaaacggg tcgactacgg ttcggtggga
      721 cagggcattg aagtcaacgt ccgggaaatg atgtcggtct tcggtctcaa ggacgaggac
      781 gtcaggatcg agcgttacgg ccgttccgaa gttgccgagg cactcaaggt tggtgactcc
      841 caggcccaca tctggactac gaatgccccg aacgcgcagg tcacggacat gatccgctcg
      901 ggcaaagtcg gtttgatcgg catcgagccg gaaaagatac aggagatcgt atcgaagttt
      961 ccccactacg ctccagttgt gattccggcg ggcgtttatg aggggtatga caaagacctt
     1021 cctgttgtcg gcgcagtggg atcgcttctc acctatgaag atatgcccga agaagttatt
     1081 tacaagatta ccaagatgat ccataccaac acgaaattcc tgcaggagag actgaactac
     1141 ttcaacaacc tgaatcttca gtttgccctt gccggaatgg ctgtgcccct tcatcccggt
     1201 gcgaagaagt actacgttga agccggcctg atcaaggaat aggaccgctc tgcggcggga
     1261 ggaaggcgcg ccggcgccgg atcctcccgc cttttgatat cgggaatttc agggaggaaa
     1321 tgatggatac tactgtttcc ggaaaccagg agaaaaaaac gtcttccggc aagaagcgct
     1381 ccttctcggg gaatctgaag cttcttgtca cggtgcttac cgttgccctg gcggtctttc
     1441 agctctacac cgcctttttc ggcgttctgc ctgccatgca gcagaggagc ctgcacctcg
     1501 cattcgtgct gccgctgatt ttccttcttt atccgatgtg gaagacatcg ccgaaaaacc
     1561 gtcccagcct tttcgactgg atgtttgcgg cgggggcggc gctttgcacc gtctatgtgt
     1621 attttatgta cgaggatatt gcgaacaggg ccggaatgta cacccagtat gaactctatc
     1681 tcggggtagt gatgattttc cttgtgttcg aagccgcacg gagggtcctg ggatacccgc
     1741 tccccatttt ctgcgccctt ttcattcttt ttgcctattt cggacgatcc atgccgggcc
     1801 ccttcaagca cttcggcctg tccatcccgc gcatcatcga agagctctat ctcaccacgg
     1861 acggtctgtt cgggctcgta accggagttt cggcaacgta catctatctc ttcattttgt
     1921 tcggggcgtt tctcagctcc acggggacat cccagttctt caatgacctt gccatggccc
     1981 ttacaggcca tctcaagggg ggaccggcaa agatcgccgt ggtttccagt gccttcatgg
     2041 gaaccatcag cggaagcacc tccgccaacg tcgccactac gggggctttt acgattcccc
     2101 tgatgaaaaa aatagggtat cagcctcatt tcgctggagc tgtggaagca gcagcctcca
     2161 ccggcgggca gattatgcct ccggtacttg gagccgccgc ttttatcatc gccgactcac
     2221 tgggaatctc gtacctcaag gttttaagtg ctgcagttgt gccggccttg ctttatttct
     2281 ggggcatctg gtgctgcctg agccttgagg ccgcaaagct cggcctgcag ggcctgccga
     2341 aggacgctct tccaagggtg aaggatgttt tggtcaagag cggatacaaa tccattcccc
     2401 ttttcgggat catctatttc ctcgtccagg ggtacaaccc gctgtacgcc ggatgctggg
     2461 gcattgtcct tgcgactctt ctcagcttcg tcaggaagga agacaggctg aatatcaggt
     2521 ccttcatttc caccctcgaa gacggctcaa aaagcgctct ttcagtggcg attgcctgtg
     2581 tcatcgtcgg cgttgtcata ggaatgatgg gcgctacggg aatcgctctg aaggtagggg
     2641 acgtcatcct tggattcacg aagggcaatc tgctccttac ccttatttcc acaatgattc
     2701 tctccatggt cctgggaatg gggatgccca cgacggcgag ctacgtcatg gcaagcgccg
     2761 tggctgctcc tgccctgatt ctcctgggag gccggcccct cgatgttcat ttcttcgtct
     2821 tcttcttcgc cgtcctgtca tcggtgacgc ctccggtatg cgtaggcgcc tatactgcgg
     2881 cgggaatagc cggctcggac ccgaacagaa cggccttcac ctcaatcaag ctcgcccttt
     2941 cgggtttcat cgtccccttc gtgttcatat actcgccgga gatcatgctc accaatgttg
     3001 aaagctggcc tgtgaccatt tttgccataa tcacggcgat tatcggcgtg ttcgggcttt
     3061 ctgtcgcgac agaggggtat atatcggcac cggttcctct ctggggaaga gtgcttgccc
     3121 tgacaggcgc cgttctcctc attttccccg gaattcttac cgatgccaca ggatttgctc
     3181 tcctcgggat ggtgatactg cataac
//
LOCUS       DAOTHD010000131         3202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_35657_length_3202_cov_3.451541, whole genome shotgun sequence.
ACCESSION   DAOTHD010000131 DAOTHD010000000
VERSION     DAOTHD010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35657_length_3202_cov_3.451541"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>892
                     /locus_tag="P5219_03245"
     CDS             <1..>892
                     /locus_tag="P5219_03245"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRX25804.1"
                     /translation="TASYGLLQHDGALLESVGWHTVVLDEAQAIKNSGTKRSAAAMKL
                     RGDFRVITTGTPVENNLGELWNLFRFLNPGLLGSLDGFTRRFAVPIEKYGNKGAAARL
                     KKLVRPFLLRRTKDQVLSELPPKTEIVLRVEMTREERAVYEAVRREATGKIESGGDGR
                     DKRFVVLAELMRLRRACCSPSLVLPEKAGLPSSRLEAFGEILEELRAGGHRCLVFSQF
                     VDHLSIIREYLDGLGVSYAYLDGSTPSRERERQIASFQGGEKECFLISLRAGGTGLNL
                     TAADYVIHMDPWWNPAVEDQA"
     assembly_gap    893..902
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <903..1094
                     /locus_tag="P5219_03250"
     CDS             <903..1094
                     /locus_tag="P5219_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25805.1"
                     /translation="HRVFRHDPVHGDGAKDTVEEKIVDLHGIKRELAESLLEGAEGAA
                     RISLEEMAALIRDDRTGKE"
     gene            complement(1131..2570)
                     /locus_tag="P5219_03255"
     CDS             complement(1131..2570)
                     /locus_tag="P5219_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRX25806.1"
                     /translation="MTPAKYSPVTERTVLDLAALVGKSNVAVDMEKRIAYSYDEVPRF
                     SWDREYIAEAVVFPETTEHVSAVLEYANRNRIPVTPRGAGTGLSGGAVPFAGGIVLSF
                     EKMRRILELDKKNLTITVEPGVVTAEITKAARQEGLLYAGDPCSGDASFVGGNVAENA
                     GGNKVVKYGTTGNSVLALEAVLPDGTVTWFGGKRRKDATGYDFAHLIVGSEGTLAVVT
                     KIILKLLPLPGKVVDLLVPFPDVASAIDFAPRIQSEGKVIPSSIEFMDGKSIRLAERF
                     LNTVLPYDDAGAHLVIQLEGNDPNVLADDMERIGDLCLASGALEVFVADNKTTSDKLW
                     KARKCLAEAIMAFYPKYSLEDLVVPTSEIPKLMAETTRLCEKYGIEEANFGHVGDGNM
                     HVNLLFPEERPDWHDIIDALLDELYDFTASIGGTLSGEHGIGLKRQKYMCRVMDGAQM
                     ELIRRVKLAFDPNEILNPGKMISCPGRGE"
     gene            complement(2567..>3202)
                     /locus_tag="P5219_03260"
     CDS             complement(2567..>3202)
                     /locus_tag="P5219_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRX25807.1"
                     /translation="EDKKNKTVHKNLLLQIRPAFGGNIIATIINYDRWPQMATVRPRT
                     FRIPEPSERKGELLTPGIPAALFESSIENLGFDRVEDGAANIADMDVVVSGGKGLKRP
                     ESFRMLEELAALLGGGVGASRPAVEAKWMGYPHQVGLSGKVVAPKVYIAAGISGAVQH
                     LAGMQTAGFVVAVNRDPDASIFRVADLGLCGDLFDILPRLTERIRKEVGAE"
BASE COUNT          554 a         1062 c         1011 g          565 t
ORIGIN      
        1 acggccagct acggcctcct gcagcacgac ggtgccctcc tcgagtcggt ggggtggcat
       61 accgtggtcc tggacgaggc ccaggccatc aagaacagcg gcacgaaacg gtccgccgcc
      121 gccatgaagc tccggggcga cttccgggtc atcaccacgg gaacgccggt ggagaacaac
      181 ctcggcgagc tgtggaacct cttccgcttt ctcaaccccg gcctgctcgg ctccctggac
      241 ggcttcacca ggcgcttcgc cgtgcccatc gaaaagtacg gaaacaaggg ggcggcggcg
      301 cggctgaaaa agctcgtccg gcccttcctt ctccggcgga ccaaggacca ggtgctcagc
      361 gagcttcctc ccaagacgga gatcgtcctg agggttgaga tgacccggga ggagcgggcg
      421 gtctacgagg ccgtccgcag ggaggcaacc gggaagatcg agtccggcgg agacgggcgg
      481 gacaagcgtt tcgtggtcct tgcagagctc atgaggctcc ggagggcctg ctgcagcccc
      541 tctctcgtcc ttccggaaaa ggcgggactc ccctcctcca ggctcgaggc cttcggcgag
      601 atcctggaag aactccgggc gggggggcac cgctgcctcg tcttcagcca gttcgtggac
      661 catctctcca ttatccgcga atacctcgac gggctcggag tgtcctacgc ctacctggac
      721 ggctccactc cgtcccggga gcgggagcgg cagatcgcct ccttccaggg gggagaaaag
      781 gaatgcttcc tcatcagcct ccgggcggga gggacgggac tgaacctcac cgccgccgat
      841 tacgtgatcc acatggaccc gtggtggaac cccgccgtgg aggaccaggc ctnnnnnnnn
      901 nncaccgtgt ctttcgccac gatcctgtac acggtgacgg ggcgaaagac acggtggagg
      961 aaaagatcgt ggaccttcac gggatcaaga gagaactcgc ggaaagcctc ctggaggggg
     1021 ccgagggggc ggcccggatc tccctggagg agatggcggc ccttatccgg gacgaccgga
     1081 cgggaaaaga gtagaaaaag gcgccccgct tccggggcgc cttccgttgc ttattcccct
     1141 cgtccgggac aggaaatcat cttgcccggg ttcaggattt cgttggggtc gaaggcgagc
     1201 ttcaccctcc ggatgagctc catctgggcg ccgtccataa cccggcacat gtacttctgc
     1261 cgcttgaggc cgatgccgtg ctcgccgctc agcgtcccgc cgatggaggc ggtgaagtcg
     1321 taaagctcgt cgagaagggc gtcgatgatg tcgtgccagt ccggccgctc ctcggggaag
     1381 agaaggttca cgtgcatatt cccgtcgccc acgtgaccga agttggcctc ctcgataccg
     1441 tatttttcgc agagcctggt ggtctccgcc atgagcttcg ggatttcgct ggtgggcaca
     1501 accaggtctt ccaggctgta cttgggataa aaggccatga tggcctccgc aaggcacttc
     1561 cgggccttcc agagcttgtc gctggtggtc ttgttgtccg ccacgaagac ctccagcgcc
     1621 ccggaggcaa ggcacaggtc gccgatgcgc tccatgtcgt ccgccagcac gttcgggtcg
     1681 ttgccctcga gctggatgac gaggtgggca ccggcgtcgt cataggggag gaccgtgttg
     1741 aggaaccgct ccgccagccg gatggacttg ccgtccatga actcgatgga ggacgggatg
     1801 accttcccct cgctctggat ccggggggcg aagtcgatgg ccgatgccac gtccgggaag
     1861 gggaccagga gatccaccac cttgccgggc aggggaagga gcttcaggat gatcttcgtc
     1921 accaccgcca gggtcccctc ggaccccacg atgagatggg cgaagtcgta tcccgtggcg
     1981 tccttcctgc gcttcccgcc gaaccatgtg accgttccgt cgggcagcac ggcctccagg
     2041 gccagcacgg aattgccggt ggtgccgtac ttcaccacct tgttgccccc tgcgttctcc
     2101 gccacgttgc cgcctacaaa agaggcgtcg ccgctgcagg ggtcaccggc gtagagcagc
     2161 ccctcctgcc gggcggcttt cgtgatctcc gcggtcacaa cccccggttc cacggtgatg
     2221 gtgaggttct tcttgtccag ctcgaggatc cgccgcatct tctcgaagga aaggacgatc
     2281 cccccggcga agggcacggc cccgccggaa agccccgtgc ccgcccccct gggcgtcacg
     2341 gggatgcggt tcctgttcgc gtactcaagc accgccgaca cgtgctccgt ggtctcggga
     2401 aagaccaccg cctcggcgat gtactcccgg tcccaggaga accggggcac ttcgtcgtag
     2461 gagtaggcga tgcgcttctc catgtccacc gccacgttgg atttccccac cagggccgcc
     2521 agatccagaa cggtccgctc cgtcacggga gaatacttcg ccggggtcat tccgcgccca
     2581 cctctttcct gatccgctcc gtaagccggg ggagaatgtc gaacaggtcg ccgcagagcc
     2641 ccaggtcggc tacacggaag atggaggcgt caggatcccg attcaccgcc accacgaaac
     2701 cggcggtctg cattcccgcc aggtgctgca ccgctcccga gatgcctgcg gcgatgtaga
     2761 cctttggggc caccaccttg ccggaaaggc ccacctggtg ggggtagccc atccacttgg
     2821 cctccacggc aggcctgctg gcccccaccc ctccgccgag aagggccgcc agttcctcga
     2881 gcatccggaa gctctcgggg cgcttcagcc ccttgcctcc cgagacgact acgtccatat
     2941 ccgcgatgtt cgccgcaccg tcctccaccc ggtcgaaacc gaggttctcg atggaactct
     3001 cgaagagagc ggcaggaatc ccgggcgtca gcaactcccc cttccgctcg gaaggctccg
     3061 gaatccgaaa cgtccgcggg cgcaccgtcg ccatctgcgg ccagcgatcg tagttgatga
     3121 tcgtcgcgat gatgttgccg ccaaacgcgg ggcgaatttg cagcaacagg ttcttgtgta
     3181 ccgtcttgtt tttcttgtcc tc
//
LOCUS       DAOTHD010000132         3202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_35658_length_3202_cov_3.166190, whole genome shotgun sequence.
ACCESSION   DAOTHD010000132 DAOTHD010000000
VERSION     DAOTHD010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35658_length_3202_cov_3.166190"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /gene="tgt"
                     /locus_tag="P5219_03265"
     CDS             <1..1076
                     /gene="tgt"
                     /locus_tag="P5219_03265"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRX25808.1"
                     /translation="RTPRGEIRTPVFMPVGTQATVKAMAPFELEEMGAQIILSNTYHL
                     YLRPGADIVAEGGGLHGFMQWKKPILTDSGGFQVFSLSTLNRISDEGVWCRSHLDGTA
                     HVMNPEWSMSVQKQLGSDIAMCFDQCAPWPCSREEAEGAVRRTALWAARSKEAHAREW
                     QGERRQALFGIVQGSVWEDLRLRSAGDIIPLDFPGYAVGGLSVGEPHEDMYRILDSLK
                     EVLPSGKPRYLMGVGRPDNLVEGVARGIDMFDCVLPTRNGRNGTLFTPSGAVNIKKKQ
                     YERDFTPLDPECDCYACRTFTRAYIRHLYRSGEILASRLCSWHNLRFLIRLGEDMRSA
                     ILEGRFPEFRRAFLGRFYGGEAE"
     gene            1073..2095
                     /locus_tag="P5219_03270"
     CDS             1073..2095
                     /locus_tag="P5219_03270"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRX25809.1"
                     /translation="MSFPRILAVDPWNPDPKVIGEAAELLRGGRLVAFPTETVYGLGA
                     NGLDPAAAAKIYAAKGRPSDNPLILHFSSPGDAESAVIVNERARRLMDLFWPGPLTLV
                     LPSRGTVPPEVTAGLDTVAVRMPSHPVALALIAAAGLPVAAPSANSSGRPSPTDAPAV
                     ASDLGDRVDLVLDGGPTSVGLESTVLDLTGKDMVLLRPGGCPVEEIESRTGEQVLRSG
                     GNVRRSPGTRYRHYAPAVPLLLHDRAGTRQKALASGGRIAWLGMDGPEILFDPGETDG
                     ELSLRFSTPENYARALFHALRFLESSGASIIVAQRPGTDAGIALALRDRLERASSEEK
                     KNSPEK"
     gene            2144..2230
                     /locus_tag="P5219_03275"
     tRNA            2144..2230
                     /locus_tag="P5219_03275"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2178..2180,aa:Leu,seq:cag)
     gene            2364..>3202
                     /locus_tag="P5219_03280"
     CDS             2364..>3202
                     /locus_tag="P5219_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRX25810.1"
                     /translation="MAEFVPVSSERQRLTFGRARDLVEIPDMIEVQRDSYNWFYQEDT
                     APEARVPQGLQELLHEVFPIESYDGSFALEFVRYLLDEPSTEEEESRQRDLTWSRPIR
                     ATIRLVNRKTKEMKEEEIFLGDFPLMTGRGTFIINGTERVVVNQLARSAGVYFTKDEQ
                     IPGQEAYSAKIIPDRGAWLEFSLTPGDLISVNIDNRKKIPATLLLKIFGASTNEGVLD
                     LFDAKKVERDVTEEDVKGMLSAESVLDQDGGTVLPRNRTITKEHLEKLWSMGRTKVLV
                     WDA"
BASE COUNT          611 a         1021 c          981 g          589 t
ORIGIN      
        1 tgcgcacccc gcggggcgag atacgcactc ctgtcttcat gcccgtggga acccaggcaa
       61 ccgtgaaggc catggcccct ttcgagctgg aggaaatggg ggcgcagatc atcctgtcca
      121 atacctatca tctctacctc aggcccggtg cggacatcgt cgccgagggg ggagggctcc
      181 acggcttcat gcagtggaag aagcccattc tcaccgacag cggcggattc caggtctttt
      241 ccctctccac cctcaaccgg atttccgatg aaggggtctg gtgcaggtcc cacctcgacg
      301 ggacggccca cgtcatgaac cccgagtggt ccatgtccgt gcagaaacag ctcggcagcg
      361 acattgccat gtgcttcgac cagtgcgccc cctggccatg ttccagggag gaggcggagg
      421 gagcggtgcg gcgcacggcc ctctgggccg ccaggtcgaa agaggcccac gcccgggagt
      481 ggcaggggga gcggcgtcag gccctgttcg gcatcgtcca gggctccgtc tgggaagacc
      541 tcaggctccg ctccgccggg gacatcattc cccttgattt tcccggctac gccgtgggcg
      601 gcctctccgt gggagaaccc cacgaggaca tgtaccgcat cctcgacagc ctgaaggaag
      661 ttcttccctc gggcaaaccc cggtacctca tgggcgtggg acggccggac aacctcgtgg
      721 agggtgtggc ccggggcatt gacatgttcg actgcgtcct ccccacacgg aacgggagga
      781 acggcaccct cttcaccccc tccggcgcgg tgaacatcaa gaaaaaacag tacgagcggg
      841 atttcacgcc ccttgacccc gaatgcgact gctacgcctg ccggaccttc accagggcct
      901 acatccgcca cctctaccgc tccggggaga tcctcgcctc caggctgtgc agctggcaca
      961 acctccggtt tctcatccgc ctgggcgagg atatgcgctc ggccattctc gagggacggt
     1021 ttccggaatt ccggcgggcc tttctcgggc gattttacgg aggtgaggcg gagtgagctt
     1081 tccccgcatt ctcgccgtgg atccctggaa tccggacccg aaggtcatag gggaggcggc
     1141 ggagcttctc cgcggaggcc gtctggtcgc ctttcccacg gagacggtgt acggtctcgg
     1201 ggcgaacggc ctcgacccgg cggcggctgc gaaaatctac gccgccaagg gacgtccctc
     1261 ggacaatccc ctgatcctcc atttttcctc ccccggggac gctgaatcgg ccgtcatcgt
     1321 caacgagagg gccaggcggc tcatggatct gttctggccg ggccccctga ccctggttct
     1381 tccttccaga gggacggtcc ccccggaagt gactgcaggg ctcgacaccg tggctgtgag
     1441 gatgccttcc catcccgtcg ccctggccct gatcgcggcg gcggggctgc ccgtggccgc
     1501 cccgagcgcc aactcgagcg gccgtcccag ccccacggac gccccggcag tggcgtcgga
     1561 cctgggagac agggtggatc tcgtcctgga cggcgggccc acttcggtgg ggctcgagtc
     1621 cacggtgctg gatctcacgg ggaaagacat ggtcctcctg cggccgggag gatgtcccgt
     1681 agaggaaatc gaatcccgta cgggagaaca ggttctccgt tccggcggca atgtgcgccg
     1741 gtccccggga acaaggtacc ggcattacgc cccggcggtt ccgcttctcc tccacgaccg
     1801 cgccgggacg cggcagaaag ccctcgcttc cggaggccgg attgcctggc tcggcatgga
     1861 cggcccggaa atcctgttcg atcccgggga gacggacggg gagctttcgc tccggttttc
     1921 cacgcctgaa aactacgccc gggctctctt ccacgctctc cggtttctcg agtcgtccgg
     1981 agcgtcgatc attgtcgccc aacggccggg aaccgacgcc ggcatcgctc ttgccctccg
     2041 ggaccggctg gaaagggcgt cttccgaaga gaagaaaaac agcccggaaa aataaggctt
     2101 gccaaaggct tcaggccttg ctaaaattac cctcgtgatt taagccgggg tggcggaatt
     2161 ggtagacgcg ctagattcag gttctagtgg gcgatagcct gtcggggttc gagtccccgc
     2221 ctcggcacca tactgaagaa aaagcagcaa ccttttttct aaaggtcgta ttatgtccgt
     2281 tgacataatg cgaccttttt gttatctttt ctatttgtca gtactcattt cccaccaatc
     2341 ttttcaggga ggtcatacgg ttcatggccg aatttgtccc cgtcagcagc gagcgccaga
     2401 gattgacctt cggacgtgcc cgggatcttg tcgagattcc cgacatgatc gaggtgcagc
     2461 gcgactcata caactggttc taccaggaag acaccgctcc ggaggcgcgg gttccgcagg
     2521 gactgcagga actgctccac gaagtgtttc ccatcgaaag ttatgacggc tccttcgccc
     2581 tcgagttcgt caggtatctt ctcgacgagc catccacaga ggaagaggaa tcccgccaga
     2641 gggacctcac ctggtcgcga cccataaggg ccaccattcg gctggtgaac cgaaagacga
     2701 aagaaatgaa ggaagaggag atcttcctcg gagacttccc cctgatgacg ggaaggggca
     2761 ccttcatcat caacggcacg gagcgcgtgg tggtcaacca gctcgcccgt tccgcaggag
     2821 tttacttcac caaggatgaa cagatccccg gccaggaagc ctattccgcg aaaatcatcc
     2881 ccgacagggg ggcatggctc gagttcagcc tgactcccgg ggatctcatt tccgtcaaca
     2941 ttgacaacag gaaaaaaatt cccgccaccc tcctgctgaa aattttcggc gcgtccacca
     3001 acgaaggcgt cctcgacctt ttcgacgcga agaaagtgga gcgggacgtc accgaggagg
     3061 acgtgaaggg catgctgtcc gccgagtccg tcctcgacca ggacggcggc acggtactgc
     3121 ccaggaacag gaccatcacc aaggagcacc tcgaaaagct ctggagcatg ggcaggacga
     3181 aggtcctggt gtgggacgcg ga
//
LOCUS       DAOTHD010000133         3201 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_35675_length_3201_cov_2.650032, whole genome shotgun sequence.
ACCESSION   DAOTHD010000133 DAOTHD010000000
VERSION     DAOTHD010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35675_length_3201_cov_2.650032"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1900)
                     /locus_tag="P5219_03285"
     CDS             complement(<1..1900)
                     /locus_tag="P5219_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRX25811.1"
                     /translation="MTIPSRKFLSLALSVLLFAALLFWMYNALAGSFGAIAENHYKKA
                     AEAAMGSVPSKDEAIGGWIRRISGPSREFDLLFLKGFPEDEGGMKVFAPTPGLGDFFA
                     HHGKDPDFARGIESAMYDEIYQPEQRFLLGGEERRVLFAPAHDGETGDVTGTAVFLFS
                     PGGFDRFLFLLRGLFLLALVLFAAVFAVAGYSRDPITGYVILGLFTLVGVFVAYPLFE
                     AVRLTFLQDGKFSLETWKSILTTRQYVTALKGSITLGAFTATASTMVGFLFAFVISRT
                     KVRGKKFFSAMATLPIISPPFSLTLSIILLFGNNGLITKQILGLENFTIYGLGGLTLV
                     QTMGMFPIAFLTMVGILEAIDSTLEDAALNLRATRWETFKTVTLPLSVPGLLSSWLLV
                     FTNSLADFANPLILSGSFRVLSVESYMEVTGMNRLGHGAALSLLLLLPTLSAFLAQRY
                     WVSRKSYVTVTGKPSGRMTELVSPGVKRILVTLMVLIVLFLFSLYGTIIAGCFVKNWG
                     IDYTFSLANFREAFERGWRVLLDTLTLAGAATPIAGILAMIAALVLARKKFPCKRLLD
                     MLILTPFALPGTLVGISYILAFNKAPLILVGTAAIIVINYVIRELPVGVEGGLASLKQ
                     IDPAIEEAA"
     gene            complement(1974..2981)
                     /locus_tag="P5219_03290"
     CDS             complement(1974..2981)
                     /locus_tag="P5219_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRX25812.1"
                     /translation="MKKIVVLAVLVLLCVTMIAGNSLAAEKVKAYATLEEPWAQELFD
                     AFEKETGIVVEWVRLSSGEAVARIEAEKANPQASIWVGGVGTLHIDAKNKGLTAPYNS
                     RMAGSIPDQFRDPEKFWIGLYVGPIAFCINTNRSAELNLPLPASWADIVKPEYEKKVR
                     VANPGTSGTAYNMVTTLIRVFGNDEDKAFEFLKKLDRSIDQYTKSGSAPGKNCAIGEI
                     PVAIGYLHDMVKLKVEGAPIEIAVPADGTGFETAALSLLKDGPDPVNGKKLYDWILGQ
                     SAMDIIATWYVIPLSDKASPVETGFSFSKMKLVNQDDVWDAANKGRLLDRWNKEIGAS
                     R"
BASE COUNT          627 a          880 c         1084 g          610 t
ORIGIN      
        1 aggccgcctc ctcgatggcc gggtcgatct gcttcagtga ggcgagccct ccctccacgc
       61 ccacgggaag ttccctgatg acgtagttga tcacgatgat ggccgccgtg cccacaagga
      121 tgaggggggc cttgttgaag gccaggatgt agctgatgcc caccagtgta ccggggaggg
      181 cgaagggcgt caggatgagc atgtcgagca gccgcttgca gggaaatttc ttcctggcca
      241 gcacgagggc ggcgatcatg gccagaatgc ccgctatggg cgtggcggct ccggcgaggg
      301 tcagggtgtc aaggagcacc ctccatcccc gttcgaaggc ctccctgaag ttggccaggc
      361 tgaatgtgta gtcgatgccc cagttcttca cgaaacaccc ggcgatgatg gttccgtaga
      421 ggctgaagag gaagaggacg atgaggacca tcagggtgac caggatgcgc ttcactcccg
      481 gcgacacgag ctccgtcatg cggcctgagg gttttcccgt gacggtgacg taggatttgc
      541 ggctcaccca gtaccgttgg gccagaaagg ccgacagggt gggcaggagg agcagaaggg
      601 aaagggcggc cccgtgcccc aggcggttca ttcccgtgac ctccatgtag gattccacgg
      661 aaaggacccg gaagctcccg gagaggatca ggggattcgc gaaatccgcc agggagttgg
      721 tgaagaccag gagccaggag ctgagcagcc cgggcaccga gagggggagg gtcaccgttt
      781 tgaaggtctc ccaccgggtg gcccggaggt tcagcgccgc gtcctccaga gtggaatcga
      841 tggcttcgag gatgcccacc atggtgagga aggcgatggg gaacatgccc atggtctgga
      901 caagggtcag ccccccgagg ccgtagatgg tgaagttctc caggccgagg atctgtttcg
      961 tgataaggcc gttgttgccg aagagcagga tgatggacag ggtgagggag aaggggggcg
     1021 agatgatggg gagggtcgcc atggctgaga agaatttctt cccccgtact tttgtcctcg
     1081 aaatgacgaa ggcgaagaga aatcccacca tggtggaagc tgtggcggtg aaggccccca
     1141 gggtgatgct gcccttcagg gcggtgacgt actgcctcgt ggtgaggatg gatttccacg
     1201 tttccaggga gaattttccg tcctgcagga aggtcagccg gaccgcctcg aagaggggat
     1261 aggccacgaa gacgcccacg agagtgaaca ggccgaggat gacatagccg gtgatggggt
     1321 ccctgctgta gcctgccacg gcgaagaccg cggcgaagag gaccagggcg aggagaaaga
     1381 gtccccgcag aaggaaaaga aaccggtcga accccccggg agagaagaga aacacggccg
     1441 ttcccgtgac gtcgccggtc tcgccgtcgt gggccggggc gaagaggacc cgccgctcct
     1501 ccccgccgag aaggaaccgc tgttcgggct ggtagatttc gtcgtacatg gcgctctcta
     1561 ttcccctggc gaaatccggg tccttcccgt ggtgtgcgaa gaagtccccg aggccgggtg
     1621 tgggggcaaa gactttcatg ccgccctcgt cctcagggaa gcccttgagg aaaaggaggt
     1681 cgaactcccg ggacgggccg ctgatccggc ggatccagcc gccgatggcc tcgtcctttg
     1741 agggaacgga gcccatggcg gcctcggcgg cttttttgta atggttttcg gcaatggcgc
     1801 cgaaggagcc cgccagggcg ttgtacatcc agaagagcag ggcggcgaaa agcagaacgg
     1861 agagggccag ggagaggaac tttcttgaag gaatggtcat ctcctccttt ccggtgaaag
     1921 aatgaagggg tcccgggcag gaccccttcc agtttcccga ttgcaggcaa actttacctg
     1981 gaggcgccga tttccttgtt ccaccggtcg aggagcctgc ccttgttggc ggcgtcccag
     2041 acgtcgtcct ggttgaccag cttcatcttc gagaaggaga agcctgtctc caccggggaa
     2101 gccttgtcgc tcaggggaat gacgtaccac gtggcgatga tgtccatggc ggactggccg
     2161 aggatccagt catagagctt tttcccgttc accggatccg gtccgtcctt caggagggag
     2221 agggccgccg tctcgaagcc cgttccgtcg gcaggaacgg cgatctcgat gggagctcct
     2281 tcaaccttca gcttgaccat gtcgtggagg tagccgatgg ccacggggat ctcgccgatg
     2341 gcgcagttct tgccgggggc ggaaccggac ttggtgtact ggtcgatgct cctgtccagt
     2401 ttcttgagga actcgaaggc cttgtcctcg tcgttgccga agacccggat cagggtggtg
     2461 accatgttgt aggccgttcc ggaggtgccg gggttggcga cccggacctt cttctcatac
     2521 tcgggtttca cgatgtcggc ccaggatgcg ggcaggggaa ggttcagctc ggcggacctg
     2581 ttggtgttga tgcagaaggc gatggggccc acgtacaggc cgatccagaa tttctcggga
     2641 tccctgaact ggtcggggat ggagcccgcc atgcgggaat tgtagggagc cgtcagtccc
     2701 ttgttttttg cgtcgatgtg gagggtgccc acgccgccca cccagatgga cgcctggggg
     2761 ttggcctttt cggcctcgat ccgggccacc gcctcgcctg aggacaggcg gacccattcc
     2821 accacgatgc cggtttcttt ctcaaatgca tcgaagagtt cctgggccca aggttcctcc
     2881 aaagtggcgt aggcctttac cttttcggcg gcgagggaat tccccgcgat catggtgacg
     2941 cacagcagaa cgagaacagc caggacaacg atttttttca tttgttcatc tcctcctgta
     3001 gtttgcgggc agcctccggt tgcgctgtct ccgcctgagt atagaaaggg aaggggtgcc
     3061 tgtcaattct tccctggccg gagaggggat ccggctctcc ttttccatgg gtggaacagt
     3121 ctccctgaaa tacacaggca aaaaggaagg gaagaattga caggcacccc ttccctttct
     3181 atactcaggc ggagacagcg c
//
LOCUS       DAOTHD010000134         3195 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_35765_length_3195_cov_3.159554, whole genome shotgun sequence.
ACCESSION   DAOTHD010000134 DAOTHD010000000
VERSION     DAOTHD010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35765_length_3195_cov_3.159554"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="P5219_03295"
     CDS             <1..1257
                     /locus_tag="P5219_03295"
                     /inference="COORDINATES: protein motif:HMM:NF022376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX25813.1"
                     /translation="VTPRTGLHHIQVGRAREVEALLKDLDRILDGGAAFRLIIGAYGS
                     GKSFFLQLVRAIALEKGLVTLHGDLSPDRRLYSTGGQARNLYAELARNLSTRTKPDGG
                     GFPGLVERFLSETRKEAEGEGTSMSTAIHVRLEKISELVGGYDFASLIEAYWNGYERA
                     DDALRANAVRWLRGEFATRTDARNALGVRTIIDDANVYDTFKILSLFVRQAGYGGLLV
                     NLDEMVNLYKLASSKARLSNYEQILRMLNDCLQGTAEAVGFLLGGTPDFLYDPRKGLY
                     SYEALQSRLSENTFAKAAGVTDYDAPILHLGSLTPEELYVLLGNIRNVYASGDPAKHL
                     VPDEALAAFLAHCSQKIGEAYFRTPRTTIKAFADFLAVLEQNPDIKWTSLIDEIPVEE
                     DRPSDMPEIGEGDGDAGGDGLTSFTI"
     gene            1265..>3195
                     /locus_tag="P5219_03300"
     CDS             1265..>3195
                     /locus_tag="P5219_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRX25814.1"
                     /translation="METSAGFSRLTEGVRRWVWKQGWPSLREIQEMALLPVLDGKEDV
                     ILSAATAGGKTEAAFLPACTRMAESSLPGTAILYVSPLKALINDQARRLESLCGSVGF
                     PVTPWHGDAPDGGKNALRKNPRGVLLITPESLESLLMNSPSWCGEAFTGLVNVIVDEF
                     HAFLGTERGCQLQSLLRRTEFLIGRTVPRIALSATLGEMEKAASALRPEKTLPCRILV
                     SEGKGSELLLQVRGYRTPRADKGVRPGLPDELVQDAYRLLRGDSHLLFTNSRSRTEEL
                     AAALSALCDRNFVPNEFFPHHGSLAPDLRRSLEERLQKKDLPTTAVCTSTLELGIDIG
                     SVASIGQVGEPGSASGLRQRLGRSGRRGAPAVLRVFVEEDETGPDSHPADALRLSLVQ
                     TAAVLSLLLRKWREPPREGLYHLSTLVQQTLSVIAQYGGVRADQLWTLLCGTGPFRAV
                     GKKDYGDFLRGLGEKHAISQLGDGRLVLGTEGELLTDRYTFFTAFSSPEEYRLEYNGE
                     LLGTVPLEIPPSPGGSLLFAGKAWETVAVIASSKTVLLRPASGGTPPRLAGGRTVVHD
                     TVRREMKQVLLGGDPPRYFDRTAAELFREGAERFRSWRLGETDILEDKGSLHLFPWRG
                     DEVVEAAAMLLRRMNLRADT"
BASE COUNT          549 a         1145 c         1008 g          493 t
ORIGIN      
        1 gtgacccccc gcacggggct ccatcacatc caggtcggcc gggcgaggga agtggaggcc
       61 ctgctgaagg atctcgaccg catccttgac ggcggagcgg ccttccggct catcatcggg
      121 gcctacggtt cgggcaaaag cttcttcctc cagctcgtca gggccatcgc cctggaaaag
      181 ggcctggtga ccctccacgg tgacctctcg ccggaccgca ggctctactc cacgggaggg
      241 caggcccgga acctctacgc cgagctggcc cgcaacctct ccaccaggac gaagcccgac
      301 gggggaggct ttcccggact cgtggagcgg ttcctctcag agacccggaa agaggcggaa
      361 ggggagggaa cgtccatgtc caccgccatc cacgtccggc tggagaaaat ctcggagctc
      421 gtgggtgggt acgatttcgc ctcgctcatc gaagcctact ggaacggcta cgaaagggcc
      481 gacgacgccc tccgggcgaa cgccgtacgg tggctccggg gagagttcgc cacccgcacc
      541 gacgcccgga acgccctcgg ggtgcgcacc atcatcgacg acgccaacgt ctacgacacc
      601 ttcaagatcc tcagcctttt cgtccggcag gcgggctacg gcggcctgct ggtgaacctc
      661 gacgagatgg tgaatctcta caagctggcc agctcaaagg cccggctctc caactacgaa
      721 cagatcctca ggatgctcaa cgactgcctc cagggcaccg ccgaggccgt gggcttcctc
      781 ctgggcggaa cgcctgactt cctctacgat ccccggaagg ggctctacag ctacgaggcc
      841 cttcagtccc ggctcagcga aaacaccttc gcgaaggcgg cgggggtgac cgactacgac
      901 gcccccattc tccacctcgg cagcctgacc ccggaggagc tttacgtcct cctggggaac
      961 atccggaacg tctacgcctc cggcgacccg gcaaagcacc tcgtccccga cgaggccctg
     1021 gcggccttcc tcgcccactg ctcccaaaag atcggcgaag cctatttccg gaccccccgg
     1081 accaccatca aggccttcgc ggacttcctc gccgtgctgg agcagaaccc ggacatcaaa
     1141 tggacctccc tcatcgacga aatccccgtg gaggaggacc ggccctccga catgcccgag
     1201 atcggggaag gggacggaga tgccgggggc gacgggctga cctccttcac aatttgaggc
     1261 cgccgtggaa acctccgccg gtttcagccg cctgaccgag ggggtccgcc gctgggtctg
     1321 gaagcagggg tggccctccc tgcgggagat ccaggagatg gccctgctgc ccgtcctcga
     1381 cggaaaggag gacgtgatcc tcagcgccgc caccgccggg ggcaagacgg aggcggcctt
     1441 ccttcccgcc tgcacccgca tggcggaatc atcccttccc ggaacggcca tcctctacgt
     1501 gagccctctc aaggccctca tcaacgacca ggcccggagg ctggaaagtc tctgcggctc
     1561 cgtgggcttt cccgtcaccc cgtggcacgg ggatgccccg gatgggggga aaaacgccct
     1621 ccggaagaac ccccgggggg ttctgctcat cacccccgaa tccctggagt ccctgctcat
     1681 gaactccccg tcgtggtgcg gcgaagcctt caccggcctg gtgaacgtca tagtggacga
     1741 attccacgcc ttcctcggca cggagagggg atgccagctc cagtccctcc tgcggcggac
     1801 ggagttcctc atcggccgga cggtcccccg gatcgccctc agcgccaccc tgggggagat
     1861 ggagaaggcc gcttccgccc tccggccgga gaaaaccctt ccctgcagga tcctcgtctc
     1921 ggaaggaaag ggaagtgagc tcctgctcca ggtgcgggga tacaggacgc ccagggcgga
     1981 caagggggtg cgccctggcc tccctgacga actggtgcag gacgcctacc gcctgctcag
     2041 gggcgattcc cacctcctct tcaccaacag ccgctcccgc accgaggaac ttgccgccgc
     2101 cctctccgcc ctctgcgacc gcaatttcgt ccccaacgag ttcttccccc accacggcag
     2161 ccttgcgccg gatctccgcc ggagtctcga agagcgcctc cagaagaagg accttcccac
     2221 tacggcggtg tgcacctcca ccctcgagct gggcatcgac atcggctcag tggcgtccat
     2281 cggccaggtg ggggagccgg gcagcgcctc gggcctccgg cagcgcctcg gccgatcggg
     2341 acggcggggc gcacccgccg tgctccgggt cttcgtggag gaagacgaaa cgggccccga
     2401 cagccacccg gccgacgctc tccgtctgtc cctggtgcag accgccgccg tcctctccct
     2461 gctgctccgg aaatggcggg aacctccccg ggaggggctc taccacctct cgaccctggt
     2521 ccagcagacc ctctcagtga tcgcccagta cggaggtgtc cgggcggacc agctctggac
     2581 gctgctctgc ggcacggggc ccttccgggc tgtgggaaag aaagactacg gcgacttcct
     2641 ccggggcctc ggggagaagc atgccatatc ccagctcggc gacgggcgcc ttgtccttgg
     2701 gacggaaggg gagctcctga cggaccggta caccttcttc accgccttct cctccccgga
     2761 ggaataccgg ctcgaataca acggtgaact cctggggacc gtccccctgg agattccccc
     2821 gtcacccggc ggttcgctcc tcttcgccgg gaaggcctgg gagaccgtgg ccgtgatcgc
     2881 ctccagcaag accgttctgc tccggcccgc ctccggagga acgccgcccc gccttgccgg
     2941 cggaaggacc gtggtccatg acaccgtcag gagggagatg aagcaggtcc ttctcggtgg
     3001 ggatcccccc cggtatttcg accggacggc ggcggagctc ttccgggaag gagcggagcg
     3061 gttccggagc tggaggctcg gggagacgga catcctcgaa gacaagggga gcctccatct
     3121 cttcccctgg aggggagacg aggtcgtcga ggcggccgcc atgctgctcc ggaggatgaa
     3181 cctccgggcg gacac
//
LOCUS       DAOTHD010000135         3194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_35790_length_3194_cov_2.710099, whole genome shotgun sequence.
ACCESSION   DAOTHD010000135 DAOTHD010000000
VERSION     DAOTHD010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35790_length_3194_cov_2.710099"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="P5219_03305"
     CDS             complement(<1..1001)
                     /locus_tag="P5219_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HRX25815.1"
                     /translation="MPKTVVKVGLASCGVAAGAVPIYETLTEALAGQKDVEIKKVGCI
                     GLCYMEPIVEVERDGKSIAYGKVDTAMAREIAESHVGRGEIVQRGVILDPESKACENE
                     RMDSQVRIVLRNSGLIDPEKIEEYIANEGYKALEKALSMTPLQVIDEVLSSGLRGRGG
                     AGFPTGLKWKFANGAKGDKKYFVCNADEGDPGAFMDRSVLESDPHSIIEGMAIGAYAI
                     GSDEGIIYCRAEYPLAIKHLNVALDQAREKGFLGKNILGSGFSFDIHIKEGAGAFVCG
                     EETAMIASIEGRRGMPRPRPPFPANSGVFGKPTNINNVETIANIPWILLNGAAKFAE"
     gene            complement(1020..1520)
                     /gene="nuoE"
                     /locus_tag="P5219_03310"
     CDS             complement(1020..1520)
                     /gene="nuoE"
                     /locus_tag="P5219_03310"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRX25816.1"
                     /translation="MTMSAATLTRSDLETRLLPLADRYRGRKGITIPLLADMQREMGY
                     IDPEAVDFIAKELDIPAAEMFGVATFYAMFRLQPEGKYVVRLCRGTACHVQGSARIGE
                     QLQRHLGVQEGETTKDGLFTLQYVACLGCCSLAPVMMVGEEVHGRLTPEKAVDVLEAV
                     RASQPE"
     gene            1689..1928
                     /locus_tag="P5219_03315"
     CDS             1689..1928
                     /locus_tag="P5219_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HRX25817.1"
                     /translation="MMIKVFSTKTCPWCTKVKEYLDGKGTAYENVDVQANREAAMEMV
                     KKTGQMGVPVTQIGEKYIVGYNPEAIDAELAKQDK"
     gene            1952..>3194
                     /locus_tag="P5219_03320"
     CDS             1952..>3194
                     /locus_tag="P5219_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRX25818.1"
                     /translation="MPRGSGTGPELLTLSYGMAVSDGESLAPGGAVDIEAVEGYEEIL
                     RRGDARLREAAAEGREGVGWLNLPGRDIADIEETGKWIAGFDALVQVGIGGSALGNLM
                     LHGALLPPYWNELPRSKRQGPRFYMSDNVDPADNRAIWNLIDPEQTVFTVISKSGSTA
                     ETMANFLFFWDRLKVDLGPKKAAEHVVVITDEEKGVLRPFADEIGCRRLVLPADVGGR
                     FSVLSPVGLLSAVALGIPAAKLLDGAADMDRKLAERDTVLENPAWLLAALSVAHYRQG
                     RNMTVLMPYEDALERFCEWFAQLWGESLGKNGTGSTPVRALGAIDQHSQIQLYTEGPD
                     DKLFTVMTVADPSEEITIPETEEKALVSLSYLFGQSMNSLRRYEALSTAAAIAKSGKP
                     VVSIEIPKLDERRLGAMIQFYE"
BASE COUNT          616 a          961 c          974 g          643 t
ORIGIN      
        1 tactccgcga acttcgccgc cccgttcagc agtatccagg ggatattggc gatggtctcc
       61 acgttgttga tgttcgtcgg cttgccgaag acgccgctgt tggccgggaa gggaggccgc
      121 ggacggggca taccccgccg tccctcgatg gaggcgatca tggcggtttc ctcgccgcag
      181 acgaaagcgc ccgccccttc ctttatatgg atatcgaagg agaatccgga ccccaggata
      241 ttcttcccga ggaagccctt ctcccttgcc tggtccaggg ctacgttcag gtgctttatc
      301 gccagggggt actcagcccg gcaatagatg atgccctcgt cggagccgat ggcgtaggcc
      361 ccgatggcca ttccctcgat gatgctgtgg ggatcgctct cgaggacgga gcggtccatg
      421 aaggcgccgg ggtcgccctc gtcggcattg cagacgaagt acttcttgtc ccccttcgcc
      481 ccgttggcga acttccactt caggcccgtg gggaagcctg ctccgccccg gccgcggagt
      541 ccgctggata ggacttcgtc gatgacctgc aggggcgtca tggagagggc tttttcaagg
      601 gccttgtagc cctcgttggc aatgtactct tcaatttttt cgggatcgat gaggccgctg
      661 ttccggagga cgatgcggac ctggctgtcc atgcgctcgt tctcgcaggc cttgctttcg
      721 gggtcgagga tgactccccg ctggacgatc tccccccggc caacgtggga ttcggctatc
      781 tccctggcca tggcggtgtc caccttgccg taggcgatgg acttgccgtc ccgctccact
      841 tcaacgatgg gttccatgta gcagaggccg atgcagccga ccttcttgat ttcaacgtcc
      901 ttctgcccgg ctagggcttc agtgagggtc tcatagatgg gcacggcgcc ggcggcaact
      961 ccgcagcttg ccaggcccac cttcacgaca gtcttgggca ttatgggcac ctccagggtc
     1021 tattccggct gggaagcccg gacggcttca agcacatcca cggctttttc cggcgtcagt
     1081 cttccatgga cctcttctcc caccatcatg acgggcgcaa ggctgcagca gccaaggcag
     1141 gccacgtact gcagggtgaa cagaccgtct ttcgtggtct ctccctcctg tacgcccaga
     1201 tggcgctgaa gctgctcacc gatgcgggcc gagccctgga cgtgacaggc cgttccccgg
     1261 cagagccgga ccacgtactt gccctccggc tggagccgga acatggcata gaaggtggcg
     1321 acgccgaaca tctccgcagc cgggatgtcc agctctttcg caatgaagtc caccgcttcg
     1381 gggtcgatgt agcccatttc cctctgcata tcggccagca ggggaatcgt aatgcccttt
     1441 cttcccctgt agcggtccgc gagagggagg agacgggtct ccagatcgga acgcgtaagg
     1501 gttgccgcag acatggtcat tccatttcct ccttgcatat tctcggtgaa ataccccgga
     1561 catggtatgc attatacccc caagaaggga atttttctct cccggtgaaa ttcggtatat
     1621 aatgaacttg acctaaagga ctttttaata tataccctat atgggtatat attttgagga
     1681 ggtgtcagat catgataaaa gtgttttcga cgaagacctg tccctggtgc accaaggtga
     1741 aggagtatct tgacggcaag ggaaccgcct acgagaacgt ggatgtgcag gctaaccggg
     1801 aagcggcaat ggaaatggtg aaaaaaacgg gccagatggg tgtccctgtg acacagatcg
     1861 gcgagaagta catcgtcggg tacaacccgg aggccattga cgcggagctg gcgaagcagg
     1921 ataagtagcc tgagaggaag gggttgacgt cgtgcctcga ggatcgggaa caggaccgga
     1981 acttctcacg ttgtcctatg ggatggcggt gagcgacggc gaatccctcg cgcccggcgg
     2041 cgcggtggat atcgaagccg tcgaaggata cgaggaaata ctccggagag gagacgcccg
     2101 ccttcgggaa gctgctgccg aagggcggga aggtgtgggc tggctcaacc tgccgggcag
     2161 ggatatcgcc gacattgagg agaccggaaa gtggattgcg ggtttcgacg ctcttgtcca
     2221 ggtgggcatc ggcggatcgg ccctggggaa cctcatgctc cacggggcgc tgctgccccc
     2281 gtactggaac gagcttcccc ggtcgaagcg gcaggggccg agattctaca tgtccgacaa
     2341 tgttgaccct gccgacaacc gggcgatctg gaacctcatc gacccggagc agaccgtctt
     2401 caccgtcatc agcaaatcgg ggagcaccgc cgagaccatg gcaaacttcc tcttcttctg
     2461 ggaccgcctc aaggttgatc tcggcccgaa aaaagcggcg gagcacgtgg tggtcatcac
     2521 cgatgaagag aagggcgttc tccggccctt cgccgacgag ataggctgcc gccgcctcgt
     2581 tcttcccgcc gacgtgggag gccgcttttc cgtgctctct cctgtggggc ttctttccgc
     2641 cgtggccctg ggcataccgg cagcgaaact gctcgacgga gcggcagaca tggacaggaa
     2701 actcgcggaa cgggacaccg ttctcgaaaa tcccgcctgg cttcttgccg ccctttcggt
     2761 ggcccattac cggcagggga ggaacatgac ggttctcatg ccctacgagg acgccctgga
     2821 gcggttctgc gaatggtttg cccagctctg gggagagagc ctcggaaaga acggaacggg
     2881 ctcaaccccc gtgagggccc tcggcgccat cgaccagcac tcccagatcc agctctacac
     2941 cgaaggtcct gacgacaagc tctttaccgt gatgaccgtg gccgacccgt ctgaggaaat
     3001 caccattccc gagacggagg aaaaagccct ggtgtccctt tcctacctct tcggccagtc
     3061 catgaattcc ctccggcggt acgaggccct ttccaccgcc gccgccatag cgaaatccgg
     3121 aaagccggtg gtctccatcg agataccgaa gctcgacgaa cgcaggcttg gagccatgat
     3181 ccagttctac gagc
//
LOCUS       DAOTHD010000136         3174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_36081_length_3174_cov_3.380571, whole genome shotgun sequence.
ACCESSION   DAOTHD010000136 DAOTHD010000000
VERSION     DAOTHD010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36081_length_3174_cov_3.380571"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="P5219_03325"
     CDS             complement(<1..291)
                     /locus_tag="P5219_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline/carnitine O-acyltransferase"
                     /protein_id="HRX25819.1"
                     /translation="MGVPAPKGGPGLPRPPFDDPVRSGEKLVEWAEPLLSESELARSR
                     EAAEEFALFGGPVLQGELDRIRNTEGDHILGRLIPYWEGWYLSSRDPLPVHSN"
     gene            complement(291..1304)
                     /locus_tag="P5219_03330"
     CDS             complement(291..1304)
                     /locus_tag="P5219_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRX25820.1"
                     /translation="MKKYAFFVLLVLCLVPASVFGTPLQASGDEPPVVFADFSWDSVQ
                     FHNRVAGFILEHGFGKKVVYSFTEEVPGFLGLERGDFHLAMETWVDNSAPYWEKAEKN
                     GRMISLGRNYEDAPQGWYVPTFLIKGDPSRGIEPSAPGLKSVSDLPRYWELFKDPEDE
                     TKGRFLNGPTGWHVSVTNAAKLQAYGLNEHFNNFHAGSASALAVGIASAYERGKAVLA
                     YYWEPTPLLGKYDMTKLEEPPYDPEIFNTTGGCDFPACRVLKTGNTEFLEAEPQIRAF
                     IERYTTTLELTNQALAFMEDNNLTHGEAAAAFLKKHPDLWRKWVGGPVAEKVAAALAN
                     SGN"
     gene            complement(1619..1846)
                     /locus_tag="P5219_03335"
     CDS             complement(1619..1846)
                     /locus_tag="P5219_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25821.1"
                     /translation="MAKKRREEREPWEYLGKKHVLWKQIIALLLSVIALFLIVVLGDF
                     LFHCFHLGRDWSDSLLVRMIVERRMILPFFR"
     gene            complement(1875..2825)
                     /locus_tag="P5219_03340"
     CDS             complement(1875..2825)
                     /locus_tag="P5219_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HRX25822.1"
                     /translation="MISVLKTFPDKKNVPLTLETLETGSWINMTAPTREEIDAVSRAV
                     KCPADFLAAALDPEEASRIEADDGCLLVVINVPKVEGNFRFDAIPMGVIITTEHIVTV
                     ALEQNDVLPSSPGALNAFSTFKRTRFLFQVLFRTATLFLKYLVQIDRRSNTIEANLRK
                     SMRNEELFQLLDLEKSLTYFTSALRSNRSVVDKLIRLCANAQMHEIVKVREEDEELLE
                     DVKIEYDQAYEMVQMYSNILSGMMDAFASIISNNLNMVMKFLASITIILAIPTVVASF
                     WGMNVHVPFGGHPLGFWSVLGIAALFAGGSAFLLWKKHML"
BASE COUNT          658 a          912 c          964 g          640 t
ORIGIN      
        1 gttggagtgg accggcagag gatcccttga cgaaaggtac catccctccc agtagggaat
       61 cagcctgccg aggatatgat ctccctcggt gttccggata cggtccagtt caccctggag
      121 cacgggaccg ccgaagaggg cgaactcctc ggccgcttct cgtgaccggg ccagttcact
      181 ttcagaaaga aggggctccg cccattcgac cagcttctct ccgctcctga cgggatcgtc
      241 gaagggcgga cggggaagcc ccggtccgcc cttcggggca ggaacgccca ttagttcccc
      301 gaattcgcga gcgcggcggc gaccttttcc gccacaggcc cgccaaccca tttccgccac
      361 aggtccgggt gcttcttcag aaacgcggcg gcggcttcac catgggtgag gttgttgtcc
      421 tccatgaacg caagggcctg gttggtgagt tcgagggtcg tggtgtaccg ctcgatgaag
      481 gcccggatct gcggctccgc ctccagaaat tccgtgttcc ctgtcttcag caccctgcac
      541 gcggggaaat cgcacccgcc ggtggtgttg aaaatttccg gatcgtaggg gggttcctcg
      601 agcttcgtca tgtcgtactt tccaaggaga ggagtcggtt cccagtaata ggcgaggacg
      661 gccttccctc tttcgtaggc cgacgctatg cccacggcaa gggctgacgc cgacccggcg
      721 tggaagttgt tgaagtgctc gttcaggccg taggcctgaa gcttcgcggc attggtgacg
      781 ctcacgtgcc accccgtggg gccgttcaga aaacggcctt tcgtttcgtc ctcaggatct
      841 ttgaacagct cccagtaacg gggcaggtct gataccgact tcagtccggg ggccgagggt
      901 tcgatgcccc tggaagggtc gcccttgatc agaaaggtgg gaacatacca gccctggggc
      961 gcatcctcgt aatttctgcc gaggctgatc atcctgccgt tcttctccgc cttttcccag
     1021 tagggagcgg aattgtcaac ccaggtctcc atggcgaggt ggaaatcccc ccgctcgagc
     1081 ccaaggaaac cgggcacttc ctcagtgaag gaatagacca cttttttgcc gaatccgtgt
     1141 tcgagaataa agcccgccac cctgttgtga aactggacgc tgtcccagct gaaatcagcg
     1201 aacacaacag gaggctcgtc tcctgaagcc tgcagggggg tgccgaaaac cgaggccggc
     1261 acaagacaca aaaccagtag gacgaaaaat gcgtattttt tcatctatct gctcctttca
     1321 tggatttagc ccttcggaag gacatagaag gcaggaaaag ccagggaaca aaatctccgc
     1381 cggactgccg gggagctgta tcgacaggat tgctgcacag atggggtact ctgcttttgg
     1441 aaaactaatg aaattattat actgcaaaaa agccgtgagg tcaaaaacgg cggcccagac
     1501 gggcaccggt gacgctcccc gctgcccgca caatgggaga tgcagtacag gggaaggccg
     1561 gagcgtccgg gggagaaagg aaaaaggcct tcccctttcg ccgggaaggc ccttgcactt
     1621 acctgaaaaa aggaaggatc atccgccgct ccacgatcat ccttaccaga agggagtcgg
     1681 accagtccct gccgaggtga aaacagtgga acaggaaatc gccgaggacg acgatgagaa
     1741 agagggcgat gaccgacagc agaagggcaa tgatctgttt ccagagcacg tgttttttcc
     1801 caaggtactc ccagggctcc ctttcttccc gtcgtttctt tgccatcttc gtgcctcctc
     1861 ccctttcggg gccattacag catgtgcttc ttccagagca ggaaggccga gcctcccgcg
     1921 aacagcgccg cgattcccag aacgctccag aagccaaggg ggtggccgcc gaagggcacg
     1981 tggacgttca ttccccagaa actggccacc acggtgggga tcgccaggat gatggtgatc
     2041 gaggcgagga atttcatgac catgttcagg ttgttggaga tgatggaggc aaaggcatcc
     2101 atcatcccgc tgaggatgtt gctgtacatc tgcaccattt catacgcctg gtcgtactcg
     2161 atcttcacgt cctccaggag ctcctcgtcc tcctcccgga ccttgacgat ctcgtgcatc
     2221 tgggcgttgg cgcagagccg gatgagcttg tccaccaccg acctgttgga ccgcagggcg
     2281 ctggtgaaat aggtaaggct cttctcgagg tcgaggagct ggaagagttc ctcgtttctc
     2341 atggattttc tgaggttcgc ctcgatggtg ttggaacggc ggtcgatctg gaccaggtac
     2401 ttgaggaaga gggtggcggt gcggaagagc acctggaaca ggaagcgggt ccgcttgaag
     2461 gtactgaagg cgttcagggc acccggggag gacgggagga catcgttctg ctccagggcg
     2521 acggtgacga tatgctccgt ggtgatgatg acgcccatgg ggatggcgtc gaacctgaag
     2581 ttcccttcca ccttggggac gttgatcacc acgagaaggc agccgtcgtc ggcctcgatg
     2641 cgggaggctt cctccgggtc cagggcggcg gcgagaaagt cggcgggaca cttcaccgcc
     2701 cggctcacag catcgatctc ctccctcgtg ggagcggtca tgttgatcca gctccctgtc
     2761 tccagggtct ccagggtcaa aggaacgttc tttttatcgg gaaaggtttt cagaacggat
     2821 atcatggaag cattcccccc ttcgcagcat tcccctctcc cggacgaatt ccgggaaccg
     2881 gggctgtgga aaagcaaggg gcgaaaaaag gagagacaaa cggaagaggc gctcctttca
     2941 cgcccggcgg gcgccggacg cgaaaaaccg tcgggacgcc ctcagccgat gtggccgcac
     3001 aacggttctg gtctcgcgct acattcgctt tccatgccgg tctcctttcc tgttcggaaa
     3061 tggattatat tatagccctt ccccttccgg aggtacaaga ggaagccggc ctaaatccac
     3121 aggcagcggg gagcgtcacc ggtgctcgct tgggccgccg gtgcagattg tcgt
//
LOCUS       DAOTHD010000137         3167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_36184_length_3167_cov_3.254499, whole genome shotgun sequence.
ACCESSION   DAOTHD010000137 DAOTHD010000000
VERSION     DAOTHD010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36184_length_3167_cov_3.254499"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="P5219_03345"
     CDS             complement(<1..586)
                     /locus_tag="P5219_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRX25823.1"
                     /translation="MDFRELQSLVEVMEKGSISAAAASLGISQPAVSKHIAKLEREMG
                     VKIFARGQKCSNLTVEGEVLYKFARKTISQLADIKRDFSDIADDVSGIVRISASSIPG
                     DFILPGLLVEFRQLYPNIEVEVRISDSREAIEKMVTRESDISITGHGRHASGFNTVPF
                     ARDELVLLVREEHPFAGRENVTLRDLENMDLVGRV"
     gene            776..1147
                     /locus_tag="P5219_03350"
     CDS             776..1147
                     /locus_tag="P5219_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRX25824.1"
                     /translation="MSLGMRIRTLRKALGLTQQALADRTEVSRIYIQALESNRRMPSM
                     KLLNRLAEALGVEVQDLVKTVSSSPSGRLQLEEVLQVSPEVEVWYRSKKLKPRELKFV
                     QSLIDAAISRWEEEDRADGMA"
     gene            1155..1571
                     /locus_tag="P5219_03355"
     CDS             1155..1571
                     /locus_tag="P5219_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HRX25825.1"
                     /translation="MNTLQAFHFPEPPEHIPEWASKAASEWAALDDFCLLAKAEERTG
                     KQIDLEYPLLPAETWGFHIVKGRRAGIFINRRLPERWKRFALFHELFHLLEHRKGEAF
                     WERTATPMSSFERQADLFAWAATLKEWETCWKESTV"
     gene            1582..2583
                     /locus_tag="P5219_03360"
     CDS             1582..2583
                     /locus_tag="P5219_03360"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRX25826.1"
                     /translation="MMRLPFFLSMQSCPRRCIYCHQGEITGVFDIPSPEHVEAAAASA
                     TKPAEVCFFGGSFTCLPPNRQKAYLDGILKAPRGSTVRLSTHPECLSPAVLALLASYP
                     VSMIELGISSLDDHVLSMCNRGYSGAVALSALESVLDAGFHGGAQMMIGLPGQSEESS
                     FDDLRRLGAARKGRPLTIRIYPCLVLKNTPLEELYRKGGYVPLDVDRAALWSGRLLIL
                     ARELGIPVQRVGLEESATLGRSIVAGPHHPAFGELARAAELAFSLAAGSSSGPWQVPR
                     KAMSLLTGHRQYGLRLLASRTGLSPEETASRVVFAGNTETNGKFPDERGIDGTLSWE"
     gene            2636..>3167
                     /locus_tag="P5219_03365"
     CDS             2636..>3167
                     /locus_tag="P5219_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRX25827.1"
                     /translation="MDSAQLQAMLREKMEQMPNKARRVVEYLLSNTREAAFLSIGEVA
                     EKLDVSKAQLVRVSRMVGFDGYADLKDALQNTVLEHVNPTALLSKIMKNRQDLPEEIL
                     RMEHANLDDTWNQLKPQNIVKFCEMVRQANMVYCMGWGISSIVAESLYTRLMEMGIRS
                     SLMKRGSVALIEQARTV"
BASE COUNT          713 a          909 c          840 g          705 t
ORIGIN      
        1 taacacggcc cacgaggtcc atgttctcga gatccctcag ggtaacgttt tccctgcccg
       61 caaaaggatg ttcttccctg accaggagaa ccagctcgtc cctggcaaac ggaacggtgt
      121 tgaaaccgga cgcatgccgt ccgtgaccgg taatggagat atccgattct ctggttacca
      181 ttttttcgat ggcttcccgt gaatcggaaa tgcggacttc cacctcgata ttcgggtaaa
      241 gctgacgaaa ttccacgaga aggcccggaa ggatgaaatc tcccggaatg gaactggcgc
      301 tgatcctgac gattccagaa acatcgtcag cgatgtcgga gaaatctctt ttaatgtcgg
      361 caagctggga aatggttttc cgggcgaatt tgtagagcac ctcgccctct acggtaaggt
      421 tggagcattt ttgcccccgg gcgaatatct tgacccccat ttcccgctcc agctttgcta
      481 tgtgcttgct tacggcaggc tgggaaattc caagagaagc cgccgcagcg gagatgcttc
      541 ccttctccat tacttccacg agactctgaa gttcacgaaa atccaacgca gattcctccc
      601 cacaaaatat gttcaatttc cctttgtttc ttccccaccc ttaatatatt ctgaacggga
      661 aaggagttca agggggtata tgattttttt actgaatgtt tttcttgaac atattgtaat
      721 cattgctata ataatattta tcctgagaaa acactccatc ggaggtggaa ttgccatgag
      781 cctcggaatg aggatcagaa cgctgcgcaa ggcccttggc ctcacccagc aggcccttgc
      841 tgacagaacg gaagtcagca gaatttatat ccaggccctc gaaagcaaca ggagaatgcc
      901 ttccatgaag cttctgaacc gcctggctga agctctcggc gtggaggttc aggacctggt
      961 gaaaacggtc tcatcatctc cttccggacg cctccagctt gaggaagtgc tccaggtcag
     1021 tcccgaggtg gaggtctggt accggagcaa gaaactcaag cccagagagc tcaaatttgt
     1081 ccagagcctc attgacgccg ctatttcccg atgggaagaa gaggacaggg cggacggcat
     1141 ggcctgatgc tttcatgaat acactgcagg ctttccattt ccccgaaccg ccggagcata
     1201 ttcccgaatg ggcctccaag gccgcctcag agtgggctgc ccttgatgac ttttgccttc
     1261 tcgccaaggc ggaagagcgg acagggaagc aaatcgacct tgaatacccg ctcctccctg
     1321 ctgaaacgtg gggatttcac atcgtgaagg ggcgaagggc aggcatattc atcaaccgga
     1381 ggcttcctga gcgctggaag agattcgctc ttttccacga gcttttccat cttctcgagc
     1441 acaggaaggg agaagccttc tgggaaagaa cggccacccc gatgtcaagc ttcgaacggc
     1501 aggccgatct ttttgcttgg gcagccacgc tcaaggaatg ggaaacatgc tggaaagaat
     1561 caacggttta ggtcgaagac tatgatgcgc ctccccttct tcctttccat gcagagctgc
     1621 ccccggcggt gcatctactg ccaccaggga gagattacag gtgtattcga cattccttcc
     1681 ccggaacacg tggaagcggc tgctgcatct gcgacaaaac cggctgaagt ctgcttcttc
     1741 ggcggcagtt tcacctgcct tcctccgaac cggcagaagg cctacctcga tggaattctg
     1801 aaagctcccc ggggaagcac cgtcagactt tccacccatc ctgaatgcct ttcaccggca
     1861 gttctcgctt tgcttgcatc ctaccccgta tccatgatcg aactggggat ttcctccctt
     1921 gacgaccatg tcctttcgat gtgcaaccgc ggctactccg gggcggtagc cctttccgcg
     1981 cttgaaagcg tcctggatgc ggggtttcac ggaggagccc agatgatgat cggccttccc
     2041 ggccaatcgg aggagagttc cttcgacgac ctccgccgcc tcggagcggc acgaaaaggc
     2101 cgtcccctga ccatccgcat ctacccgtgc ctcgttctca aaaacactcc cctggaagaa
     2161 ctctaccgga aaggaggcta tgttccgctg gacgtcgacc gggcagccct ctggtccggc
     2221 aggctcctca tccttgcccg ggagctgggt attccggtcc agcgggtggg actcgaggaa
     2281 tcggcgaccc tcggccggtc catcgttgcg ggccctcatc atcccgcctt cggtgaactt
     2341 gcccgggcgg ccgaactggc cttttctctt gctgccgggt cttcctccgg gccgtggcag
     2401 gttccccgaa aagcgatgtc gcttctgacg ggccaccggc agtacggact ccgcctcctg
     2461 gcctccagga cgggcctttc acccgaagaa accgccagca gggttgtctt tgccgggaat
     2521 accgaaacaa acgggaagtt ccccgacgaa agaggaattg acggaaccct ttcttgggaa
     2581 taaacttaaa tctccatata attcctggac tattatccgg tttcggaggt gtcttatgga
     2641 cagtgcacag cttcaggcca tgctgcggga aaaaatggag cagatgccga acaaggcccg
     2701 gcgcgttgtg gaatatcttc tttccaacac cagggaggcg gcatttcttt ccattggaga
     2761 agtggccgaa aagctcgacg tttcaaaggc ccagcttgtc cgtgtttcaa ggatggtcgg
     2821 tttcgacggg tacgccgatt tgaaggacgc tctccagaac acagtcctcg agcacgtcaa
     2881 ccccaccgct cttttgagca agatcatgaa aaaccgacag gacctccccg aggaaatcct
     2941 cagaatggaa catgcgaacc tggacgacac ctggaaccag ctcaagcccc agaacatcgt
     3001 gaagttttgt gaaatggtcc gccaggcgaa catggtctac tgcatgggct ggggcatatc
     3061 ttccatcgtg gccgagtccc tgtacacccg cctcatggag atgggcatcc ggagctcact
     3121 gatgaaaagg ggttcagtcg ctctcattga gcaggcaaga acggtga
//
LOCUS       DAOTHD010000138         3166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_36205_length_3166_cov_2.691096, whole genome shotgun sequence.
ACCESSION   DAOTHD010000138 DAOTHD010000000
VERSION     DAOTHD010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36205_length_3166_cov_2.691096"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2569
                     /locus_tag="P5219_03370"
     CDS             <1..2569
                     /locus_tag="P5219_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRX25828.1"
                     /translation="GFQGSAPQAVPQIIAAREGERALASKILSEKNAPCPYYFPEHLR
                     KALSAIPDYPVTSIEADSGYGKTTALREFLRNLPGNSRAVWHTCFGEPPAKTWESICR
                     MFGEVDAEVSRQLKELYPPTMETLSDIAASVHELGCGEETFLVIDNYQLFRNTIPSRL
                     MDAFSVHTNPHVHIVFVTQPLAASPMSVHNPNIHRIGTKDFLFDRHSIAQYCRGAKID
                     ISEREIEYIKSISEGWIAAICLQTDNYRRTGSLVKTNDMNFLIENTVWNRVPGRSREF
                     LTALSLLDGFTLKQAAIMGNRQSVPDDMVRLLRGNFFIPYVEDKGVYSMHSLLRDYLR
                     SRFDEFEPEIRRELLHRAGIALSEAGDLFGASRFYFESGDYGAVIKLPFTVRYFYNNF
                     DVGMLGFLERFVKECPAEVIGGNPMFLVMAAYFMFRNGRREQFNELAGLIRSLLDDPN
                     RALDRDFLKIKGEFALLMSFSRFNDIVEMSAYHREALRFFEAAGECPPRSSFFGGNIP
                     WTFGCSSVFSLYWRTSGDLDRELEVMDECVPYYVTISGGHGAGGEHVMRAEAMLMRGD
                     DRAAEVAAHRAIYRASEENQIGNRICAELVLARLAILRGNRAAYISLREELTARARNA
                     GQRAVFLLGELARASLDAVLGLRDEIPDWLLTADGINNMLFLHARPYAFSILGALLLA
                     DRRYTELEALTDPVMDLSRRVKFLLPRVSHLLYLSAAEKREGRKRAALEKLSAALEMT
                     LPDRVCLPFAEFGNELVPLLVELKGTFDSEKMDSIIALCERFSEGAANIVRQAEGGRS
                     ALAPREREIALLVKEGFQTGEIAARLFISENTVKSARKVIYGKLGIHSRAELKKITL"
     gene            2899..3144
                     /locus_tag="P5219_03375"
     CDS             2899..3144
                     /locus_tag="P5219_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25829.1"
                     /translation="MMETDTHIVFDFTSRLGTMRFGALVDDELFSSARTDGNFILFGK
                     PGAPEIRIGPADFADLVLVDRTMDGPDYGSIFSKELY"
BASE COUNT          697 a          867 c          922 g          680 t
ORIGIN      
        1 cgggtttcag ggatcggccc cgcaggcggt gccacaaatc atcgcagccc gtgaaggaga
       61 aagagcgctg gcctccaaaa tcctttctga aaaaaacgcc ccatgcccgt actattttcc
      121 cgaacacctg agaaaagccc tctctgcgat cccggactat ccggtcactt ccatcgaagc
      181 ggactccggt tacggcaaga cgaccgcact gcgggaattt ctgcggaacc tgcccggaaa
      241 ttcccgtgcc gtctggcata cctgcttcgg tgaacctccg gcgaagacat gggaaagcat
      301 ctgccgcatg ttcggggagg tggacgcgga agtctcgcgg caattgaagg agctgtaccc
      361 cccgacgatg gaaacgctct ccgacatcgc cgcgtcggtg cacgaacttg gctgcggcga
      421 agaaacattt ctcgtcatcg acaattacca gctcttccgg aacaccatac cgtccaggct
      481 gatggatgcc ttctccgtgc acacgaatcc ccatgtccac atcgtcttcg tcacccagcc
      541 ccttgcggca agccccatga gcgtccacaa ccccaacatc cacagaatcg gcacgaagga
      601 tttcctgttc gaccggcaca gcatcgccca atactgccga ggggcaaaaa tcgatatttc
      661 cgagagagag atagagtaca taaaaagtat ttccgagggg tggatcgccg ccatatgcct
      721 ccaaaccgac aactacagaa gaacgggttc tctggtgaag acgaacgaca tgaacttcct
      781 catcgaaaat accgtgtgga acagggtgcc cgggagaagc cgcgaatttt tgacggccct
      841 ctctctgctc gacggcttta ccctgaaaca ggcggccata atggggaacc ggcagtccgt
      901 cccggacgac atggtccggc tgctcagggg taattttttt attccgtacg tcgaggacaa
      961 aggcgtgtat tccatgcaca gcctgttgcg ggattatctg cggagccgtt tcgacgaatt
     1021 cgagcctgaa atccggcgtg agctgctgca ccgggctgga atcgccctgt ccgaagccgg
     1081 agacctcttc ggcgcgtccc gtttttattt cgagtccggg gattacgggg cggtaatcaa
     1141 gctgcccttc accgtgcggt atttttacaa caatttcgat gtcggcatgc tcggctttct
     1201 cgaacgattc gtgaaggaat gcccggcgga ggtgatcggc gggaatccca tgttcctggt
     1261 catggcggcg tatttcatgt tcaggaacgg gcggcgggaa cagttcaatg agcttgcggg
     1321 cctgatacgc tcgttgctgg acgatccgaa ccgcgcgctt gacagggatt tcctgaaaat
     1381 aaaaggggag ttcgcgctgc tgatgtcgtt ttccaggttc aacgatatcg tggagatgag
     1441 cgcgtatcac agggaggccc tgcggttttt cgaggcggcg ggggaatgtc cgccccggtc
     1501 gtcatttttc ggcggcaaca ttccgtggac attcggctgt tcgtcggtat tctcccttta
     1561 ctggcgcaca tccggcgatc tggacaggga actcgaagta atggacgagt gcgtgcccta
     1621 ttacgtgacc atttccggag gacacggcgc cggcggggag cacgtcatgc gtgcggaggc
     1681 gatgctcatg cggggcgacg acagggcggc ggaagttgcg gcccacagag cgatttatcg
     1741 cgcttcggag gaaaaccaga tcggcaatcg catctgtgcg gaactcgttt tggcgaggct
     1801 cgcaattctg cgcgggaaca gggcggcgta catttccctt cgggaggaac tcacggctcg
     1861 tgcacggaac gccgggcaga gggcggtgtt tttgctcggc gaactggccc gggcttctct
     1921 cgacgctgtt ctcggcctgc gggacgaaat ccccgattgg ctgcttactg cggacggcat
     1981 caacaacatg ctgttccttc acgcccggcc gtacgcattt tccatcctgg gcgcactgct
     2041 gctggctgac cgccgttaca cggaactcga agccctcacc gacccggtga tggacctgtc
     2101 ccgcagggtg aagtttctgc tgccccgggt cagtcatctc ctgtatctgt cggcggcgga
     2161 aaaacgggag ggaaggaaac gggcagcatt ggagaaacta tctgccgcgc ttgagatgac
     2221 cctgcccgac agggtctgtc ttcccttcgc ggagttcgga aacgaacttg tgccgctgct
     2281 cgtggaactg aagggcactt tcgattcgga aaaaatggac agcatcatcg cgctctgtga
     2341 aagattttcg gaaggagcgg cgaatatcgt cagacaggcg gagggcggaa ggtcggctct
     2401 tgcgccccgc gaacgggaaa tcgccctgtt ggtgaaggaa ggtttccaaa cgggtgaaat
     2461 cgcagcccgg cttttcatat ccgaaaacac tgtaaaatca gcccgcaagg ttatttacgg
     2521 caaactcgga attcattcga gagcggagct gaaaaaaata acgttgtaaa ggggccggca
     2581 ttttcccctg ttcttcttca gcatcaaact tccggacggc aggtttggtt cgaaaaatca
     2641 tgtttttctt cccttcgaaa tacggcattc cgaagttaat ggtcacccct cctgaaaccc
     2701 cccctttcgg gggaggcggg aaaattttct ctcagtacaa tggtgcataa cagaaaaata
     2761 aatgcctgac acaaaatagg tcccgtacaa ctgcggggac actgttgttg tgttgcctca
     2821 acgcgggagg atgccatgga gacacaatcg ggttttttta gaagcgtaca ccctctgcca
     2881 atgcacacat tggaggtcgt gatggagacg gacacccata tcgtgttcga tttcacatcg
     2941 cgcctgggca ccatgagatt cggggcgctc gttgacgatg aattgttcag cagcgcccga
     3001 acggacggga attttatcct gttcgggaaa cccggagcgc cggaaatccg catcggaccg
     3061 gcggacttcg cggacctggt gctcgtggac cggacgatgg acggtccgga ttacgggtcg
     3121 attttctcaa aagaattata ttaatgagga gtgagctgta tgaagt
//
LOCUS       DAOTHD010000139         3165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_36223_length_3165_cov_3.295820, whole genome shotgun sequence.
ACCESSION   DAOTHD010000139 DAOTHD010000000
VERSION     DAOTHD010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36223_length_3165_cov_3.295820"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="P5219_03380"
     CDS             complement(<1..429)
                     /locus_tag="P5219_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HRX25830.1"
                     /translation="MRSSSCPVPEEALEPLLLLGKKIEEKLGLVCTDKRRADIWRSVA
                     RTASARSESPLGVLLGLAGASPEEELPVDFVADLTVGETFFFRERKALGMFSETALPE
                     IAAGKGTAPIRIWSAGCSSGEEPYTLSMLLLNALPGMDPEQ"
     gene            complement(426..911)
                     /locus_tag="P5219_03385"
     CDS             complement(426..911)
                     /locus_tag="P5219_03385"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRX25831.1"
                     /translation="MNRKSFLVFSIGEKRFALPSEDVFRVSPAAELLEVPEAPGFIRG
                     VVNLGGEPVPVADLRKKKGLSFREMELSDRFVFFRSGGTLCGVLAESVEGVLDLVPET
                     VPFSSLLVRQGDPLPGTVRVAYGEESGAILIRSPENLLSITEEELRCLEPVMSARREA
                     L"
     gene            1125..1868
                     /locus_tag="P5219_03390"
     CDS             1125..1868
                     /locus_tag="P5219_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRX25832.1"
                     /translation="MKKLLLALLVLALAATGGWADVMAKDVILVGTESTYPPYEFRDE
                     KNNLKGFDIELMEAIAAKIGKKIEWVDMPFDSLIPSLLAKKIDIVAAGMSATEERAKK
                     VAFSENYEISISAFIVKADNDSMKVLGDMKGKTVAVQLGTVQETYSQSIPGVTVKSFQ
                     KFDDCVREVILGRVDATLMDTPVAKSYVEHKDFTGKIKIAFEQEITGSGKALAMNLGE
                     TAFITAVNAALADLEKSGELGKMKEQWFK"
     gene            complement(1919..>3165)
                     /locus_tag="P5219_03395"
     CDS             complement(1919..>3165)
                     /locus_tag="P5219_03395"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HRX25833.1"
                     /translation="AAYTPGKKDRTLDASPGHRDSPFPGRTASGGNSREALAAPIGSP
                     RLSSLASGAGKIAIVLPDATRLWQDVPRMACAIRREIGEGKAAVTWIIGAGLHRAPTE
                     EEKNLLLGGASRPGETVIWADPARAADTGMVTSRGTPVTVAPEALGADLLVLAGGIVY
                     HDLAGFSGGRKGLLPGISGRVSVQNNHGLSLRGGMEGLEVNVGRLSGNATAEDMEEYG
                     RLLEKGRTIFLLNVVPDETGKPHCYTAGSLDEAWSRGVEMARALQTLHIPGKTALVVV
                     SSGGYPYDIDLYQATKAVTASLGALKPGGGLILCAGLEDGLGPGNFASDLPLALCDPG
                     ALLRRLEADFTIPGFIALKVPHDMKGHPAALVTEKEGTPFPGETFTSFDAAMEWILPR
                     VPPGPAVYVRSGNCIVLRADSE"
BASE COUNT          649 a          964 c          965 g          587 t
ORIGIN      
        1 ctgctccggg tccattccgg gaagagcgtt caggagaagc atggagaggg tgtagggctc
       61 ctcgccggag ctgcacccgg cactccagat ccggatgggg gcagttccct ttcctgcagc
      121 aatttccggc agggcggtct cggagaacat cccgagggct tttcgttccc tgaagaaaaa
      181 ggtctctccc acggtcaggt cagcgacgaa gtcaacaggc agttcctctt cgggtgaagc
      241 accggcaagt ccgagaagaa ctccgagagg agattccgac cgtgctgacg ctgttcgggc
      301 cacggaacgc cagatatcgg cccttcgttt gtccgtgcat acaagtccga gtttctcttc
      361 tatttttttc ccgaggagca ggagaggctc cagggcttct tccggtacgg gacaggagga
      421 ggatctcaca gggcttctct ccgggcggac atgaccggct ccaggcagcg aagctcctcc
      481 tcggtaatgg aaagaaggtt ttccggcgac cgaatcagga tcgccccgga ctcctccccg
      541 taggccaccc ggacggtgcc cggcaggggg tcaccctgcc gcaccaggag agaagagaag
      601 gggacggtct ccggaacgag atccaggacg ccttccacgg actccgctag aaccccgcag
      661 agggttcctc cgctgcggaa aaacacgaac cggtcgctca gttccatctc ccggaaggaa
      721 agacctttct ttttcctgag gtccgccacg ggaaccggct cccctccgag gttgacaaca
      781 ccacggatga acccgggggc ttcgggcacc tcgaggagtt ccgccgccgg ggacacccgg
      841 aacacatcct ccgagggaag ggcgaatcgc ttttcgccta tggaaaaaac aagaaagctc
      901 ttccggttca ccgcagcttc cccctttcaa tccccgcagc acacattttc cgctcagggt
      961 aatcggcccg aaattctgtg ctcatgattc attatacagt cctgcagaaa aaggggaaag
     1021 tcggtattga caggaaaaaa cggggcgtgt atcctgactg tacattgaat atcattcgtg
     1081 aagaatgata taaacatatg ttcaatcacg aaggagagat catcatgaaa aaactgctgc
     1141 tcgcgcttct ggtcctggcc ctcgcggcca ccggcggatg ggccgatgtc atggccaagg
     1201 acgtgattct tgtcggaacg gaaagcacct accccccgta cgagttccgc gatgagaaga
     1261 acaatctcaa gggtttcgac atcgaactga tggaagccat cgcggcaaag atcggcaaga
     1321 agatcgagtg ggtggacatg cccttcgaca gcctcattcc ctccctgctc gcgaagaaga
     1381 tcgacatcgt tgccgccgga atgagtgcta cggaagagcg ggcaaagaaa gtcgccttct
     1441 cggagaacta cgaaatctcc atcagcgcct tcatcgtgaa ggctgacaac gactccatga
     1501 aggtcctcgg ggacatgaag ggaaaaaccg tggcggtcca gctcggcacc gttcaggaga
     1561 cctattccca gtccattccc ggcgtcacgg tgaagtcctt ccagaaattc gacgactgcg
     1621 tccgtgaagt catcctcggg agggtggacg ccaccctcat ggacaccccc gtggcgaaga
     1681 gctacgtgga gcacaaggat ttcaccggga agatcaagat cgctttcgag caggagatta
     1741 caggttccgg aaaggctctg gccatgaacc tcggcgagac ggccttcatc acggcggtca
     1801 acgccgccct ggcggatctt gagaagagcg gagagctcgg caagatgaag gagcagtggt
     1861 tcaagtagga ttcccgcaat caaaagcccc cggcccgttt aacgggccgg gggctttttc
     1921 attcgctgtc cgccctgaga acgatgcagt ttccggaccg cacgtagacc gcggggcccg
     1981 ggggaaccct tggaagaatc cactccatgg cggcgtcgaa ggaggtgaag gtctctccgg
     2041 gaaaaggcgt tccttctttc tccgtcacca gggcagccgg atgtcccttc atgtcgtggg
     2101 gcaccttgag ggcgatgaag ccgggtatgg tgaagtctgc ttccagccgg cggagaagag
     2161 ccccggggtc gcagagtgcc agggggagat ccgaggcgaa gttccccggg ccgaggccgt
     2221 cttcgagccc cgcgcagaga atgagccccc ctccgggctt cagggcgccg agagaggcgg
     2281 tgactgcctt ggttgcctgg tacaggtcga tatcgtaggg gtatccgccg cttgacacca
     2341 ccacgagggc cgtcttcccg gggatgtgga gcgtctggag cgcccgagcc atttccactc
     2401 ccctgctcca cgcttcgtcc aggcttcctg ccgtgtagca gtggggtttg cccgtctcgt
     2461 ccggaacaac attgagcagg aagatggtcc ttcctttttc aagaagccgg ccgtattctt
     2521 ccatatcctc ggcggtggcg tttcccgaga gccgtcccac gttcacctcc aggccttcca
     2581 tccctccccg gagggatagg ccgtggttgt tctggacgga aaccctgccg ctgatgccgg
     2641 gcagaagccc ctttctcccg ccgctgaacc cggcgagatc atggtagaca ataccccccg
     2701 caaggacaag cagatccgcc ccgagagcct caggggccac cgtcacagga gttccccggg
     2761 atgtgaccat acccgtgtcg gcggccctgg ccgggtccgc ccagatgact gtttccccag
     2821 gccgggaggc cccgccgaga agcaggtttt tctcctcctc cgttggagcc ctgtgaagcc
     2881 cggcgccgat gatccacgtc acggcggcct tcccttcgcc gatctcccgc ctgatggcgc
     2941 aggccattcg gggcacgtcc tgccacagcc tggtggcgtc gggaagcaca atggcgatct
     3001 tccctgctcc ggaggccagg gacgacagcc ggggggagcc gatgggggcg gcaagggcct
     3061 ctcgggagtt cccgcctgag gccgttcgcc ccgggaacgg ggaatcacga tgtcccgggg
     3121 aggcgtccag ggtacggtct ttcttcccag gggtatatgc agcat
//
LOCUS       DAOTHD010000140         3164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_36240_length_3164_cov_3.325828, whole genome shotgun sequence.
ACCESSION   DAOTHD010000140 DAOTHD010000000
VERSION     DAOTHD010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36240_length_3164_cov_3.325828"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..1176)
                     /locus_tag="P5219_03400"
     CDS             complement(109..1176)
                     /locus_tag="P5219_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRX25834.1"
                     /translation="MNGNGNNLARASRNLLGDIGLPTVIIAVFWVLTLVAGHFVGISM
                     STLVSDTIKRAGMNGILVLAMVPAIQSGTGLNFALPIGIVCGLLGLVTAIEMDLTGMT
                     WLLTSFALALPLAAAVGYGYGKLMNAVKGSEMTIATYTGFSIVALMCLAWLMIPFRNP
                     KMGWFIGKGLRETIQLDAVGGAQILNNFLLFTVGDVIVPTGLLLTFAFFAFLVWLFFR
                     SKLGIAISAGGINPKFAQAAGLNIDRNRVLANVVSTILGAIGIIVYSQSYGYAQLYTA
                     PLMMAFPAVAAILIGGATASRAKVSHVVIGVVLFQGLLTTALPVANEIFVGTDLSEIM
                     RMIIQNGIILYALTQVKRGVQ"
     gene            complement(1178..2794)
                     /locus_tag="P5219_03405"
     CDS             complement(1178..2794)
                     /locus_tag="P5219_03405"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRX25835.1"
                     /translation="MSDSLLQFRNISKSYYGNSVLANINFDVRKGEIHALIGENGAGK
                     STLMNILFGMPVITGTGGYEGDVLIDGEKISFKSPHEAMYAGIGMVHQEFMLIPGYSI
                     TENIKINREITTPNPVSRIFGKRLETLDMASMNADARKALDTLDMGIEEFTMVAGLPV
                     GHMQFVEIAREIDKTGIKILVFDEPTAVLTESEAQNLLAAVKRLAAKGIGIIFISHRL
                     DEIISVADRVTILRDGELVATKDIKDTDAVEIAALMIGRKVAIDRVQSDRRRISDEII
                     LSLKDLHVGMPGEMVRGIDLDIRKGEIVGIGGLAGQGKLGIANGIMGTYPARGDVVFN
                     GKPMILGDPRAAMASGMGFVSEDRKGVGLLLNESIELNIAFTAMQVGNKFLKQGGPFR
                     FQDDDGIRKHALKMIKDLDIRCESPVQHAGSLSGGNQQKVCVARALTQDPVFLFVSEP
                     TRGIDIGAKKLILDLLLKLNEEEGMTIVMTSSELAELRSICDRIVIVCEGKVEGVLPP
                     DASDAEFGLMMSGSRGGKTEDSPERSADHG"
BASE COUNT          633 a          909 c          936 g          686 t
ORIGIN      
        1 cgctggagac catgaagccg aagagggtaa aagccacgaa gataatggtc accaggtttt
       61 caagaatcca ggatcctgtg ccgttcgagt tcctgttgag tccgtccatc actgcacacc
      121 tctttttacc tgggtcaggg cgtaaagtat gattccgttc tggatgatca tccgcatgat
      181 ttccgacagg tcggtgccga cgaagatttc gttggccacc gggagggccg tggtgagcag
      241 tccctggaag aggacgacgc cgatgaccac gtgggacacc ttcgcccggg aggcggtggc
      301 tccgccgatg aggattgccg ctacggcggg gaaggccatc atcaggggtg cagtgtagag
      361 ctgagcgtac ccgtagctct gggagtagac gatgatcccg atggcgccga ggatggttga
      421 aaccacgttt gccagcaccc gattccggtc gatgttcaga ccggcggcct gggcgaattt
      481 cggattgatg cctccggcgg aaatggcgat tccgagcttc gacctgaaga aaagccagac
      541 gaggaaggcg aaaaaggcaa aggtaagaag aagacccgtg ggaacgatca catcacccac
      601 ggtgaacaga agaaagttgt tgaggatctg ggctcctccc acggcgtcga gctggatggt
      661 ctcccggagc cccttgccga tgaaccagcc cattttcggg ttgcggaagg gaatcatgag
      721 ccacgccagg cacatgaggg ccacgatgga gaagcccgta taagtggcga tggtcatttc
      781 ggaacccttg acggcgttca tcagcttgcc gtagccgtag cccacagcgg ccgcgagggg
      841 taaggcgagg gcgaaggatg tcaggagcca ggtcattccc gtcaggtcca tctcgatggc
      901 ggtgacgagg cccaggagcc cgcagacgat gccgatggga agggcgaagt tcagccccgt
      961 gcccgactgg atggcgggca ccatggcgag gacgaggatg ccgttcattc cggcccgctt
     1021 gatggtgtcg ctcacgaggg tggacatgga aatccccacg aagtggccgg cgaccagggt
     1081 gagtacccag aagacggcaa tgatcaccgt ggggaggccg atatctccca gcaggttccg
     1141 ggaggcccgc gcaaggttgt ttccgtttcc gttcatctca gccatgatcg gcactcctct
     1201 ccggtgaatc ttccgttttt ccgccccggc tgccggacat catcaggccg aattccgcgt
     1261 cggaagcatc gggcggcagc acgccttcca ccttgccctc gcagacgatg acgatcctgt
     1321 cgcagatgct ccgcagctcc gccagttcgg aagaggtcat gacgatggtc atcccctcct
     1381 cttcgttgag cttcagcagc aggtcgagga tcagcttctt cgcgccgatg tcgatgcccc
     1441 tcgtgggctc cgagacgaaa agaaagaccg gatcctgggt gagggccctg gccacgcaga
     1501 ccttctgctg gttgccgccg ctcaggctgc cggcgtgctg gacgggggac tcgcagcgga
     1561 tatcaaggtc tttgatcatc ttcagggcat gcttccgaat cccgtcgtca tcctggaagc
     1621 ggaaagggcc tccctgcttc agaaatttgt ttcccacctg catggctgta aaggcgatgt
     1681 tcagctcgat ggactcgttg agcagaaggc cgacgccttt cctgtcctcg ctgacgaatc
     1741 ccatccccga ggccatggcg gcacgggggt cgcccaggat catgggcttg ccgttgaaga
     1801 ccacgtcgcc cctggccgga taggttccca tgatgccgtt ggcaatgccg agcttgccct
     1861 gccccgcaag accgccgatg ccgacgatct cgcccttccg gatgtcgagg tcgatgcccc
     1921 ggaccatctc gccgggcata cctacatgaa ggtctttcag gctcaggata atttcgtccg
     1981 aaatcctgcg cctgtcggac tggacccggt cgatggcaac ctttcggccg atcatcaggg
     2041 cggcgatttc cacggcgtcg gtgtctttta tgtcctttgt ggccacaagc tcaccgtccc
     2101 ggaggatggt gaccctgtcg gccacgctga tgatctcatc aagcctgtgg ctgataaaga
     2161 taatgccgat gcccttggca gcaagccgtt tgaccgctgc caaaaggttc tgtgcctccg
     2221 attcagtgag taccgccgtg ggctcgtcga aaacgagtat tttgatccct gttttgtcga
     2281 tttcccgggc tatttcgacg aactgcatgt gccccaccgg aagccccgca accatcgtaa
     2341 actcctcaat acccatgtcg agcgtgtcca gggcttttcg ggcatcggcg ttcatggagg
     2401 ccatgtcgag ggtttcaagg cgttttccga agatccggga gaccgggttc ggcgttgtga
     2461 tttccctgtt gatcttgatg ttttccgtaa tggagtagcc tggtatgagc atgaattcct
     2521 ggtggaccat gcctattccg gcatacattg cctcgtgagg ggacttgaag ctgattttct
     2581 ctccgtctat caggacgtcg ccctcgtatc cgccggtgcc cgtaatgacg ggcatgccga
     2641 agaggatatt catcagcgtg gattttccgg cgccgttctc tccgatgagg gcgtggattt
     2701 cgcccttcct gacatcaaaa ttgatgtttg ccagaacgct gtttccataa tagctctttg
     2761 aaatattcct gaactggaga aggctgtcgc tcaacgcatt accccctgta tttcaaaatc
     2821 ggcatgacgg ccgcaaaaaa agcgggggcg aacggattca acccctgcgg tccggaccgg
     2881 aagcgctccg aaaaaatcgc tcccaagcct cccgcttcgt tgaaaaatcc gctcgtcccc
     2941 ggacaccgct cttgctgcag cctgttgtgg atataacttt cgcctggtac agcctctttt
     3001 acccttagta ggtcaggaag tccatcagga cgaacaggaa gttgtcgtat ttctttcccg
     3061 cttcgtcaac atagggattc gatttgaccg cgaccttcgc gtaatcggcc atgcactgct
     3121 ccaggacctt gatgtcgagc gtgtcgaaag gaacttcgcc gctg
//
LOCUS       DAOTHD010000141         3163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_36258_length_3163_cov_3.282497, whole genome shotgun sequence.
ACCESSION   DAOTHD010000141 DAOTHD010000000
VERSION     DAOTHD010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36258_length_3163_cov_3.282497"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="P5219_03410"
     CDS             <1..490
                     /locus_tag="P5219_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRX25836.1"
                     /translation="ASYIYIIRGTPLLIQLFLIYFGLPSLGLNLPAFTAGIIGLSINS
                     SGYVGEIVRGGIEAVPKGQWEASKVLGLSYLKTMRFIILPQAIRNMLPAIGNEFVTLI
                     KESSLLSTLAITELTMVGQQVRSVTYASFETFIVVGIIYLVLTSVTSFSLQYVERKWK
                     VS"
     gene            complement(551..1471)
                     /locus_tag="P5219_03415"
     CDS             complement(551..1471)
                     /locus_tag="P5219_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25837.1"
                     /translation="MIFEFMSRGNIARLPDGMIVDGLPEGPFPRVLLLLPYNRYLVGS
                     ATMKHYERWTLGKLGTDESTEVFLYEGKPKTLLLGEVEKVTISVEVISQLKSCLSGQM
                     PPPGEHMPSALILRGLIGEDHLLGEGEFLHNEEAFFDVLEKDGMAKMAYWAIRLALFR
                     NDYEAVSRVKTWMKSAADVFEGVTQPPKIWFSLTDLPGKRDIEEMEGLAFSLDDLQRM
                     NSQSSRPVVLYSKSGYLILSDFGGEGPDSAFRIWMFLPISLWNEMRERRKLSIREIVM
                     ASWGYLDGLSAEKERSRYAQRTEAQGTRMV"
     gene            complement(1595..2374)
                     /locus_tag="P5219_03420"
     CDS             complement(1595..2374)
                     /locus_tag="P5219_03420"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRX25838.1"
                     /translation="MASFFSCGLRVIRPRRKVALDNLRLAFPDSDESWRKETVTKCYQ
                     HISWMVAEYLSLVSDPSRVHSWIVETEGKEILDELRDSGRGAIILTGHVGNWELLAGW
                     LAQSGYPLTAVVRNPDDPNLSELIETYRAALGVGTIEKHFIMKDAVRLAKKGGFLGLL
                     PDQAWHSSGVRGPFFGRMCYTAGGPAAIAHLAGVPVVPVVSYRLAPFRHRVVISPPLP
                     MAEGTDRNSAIHENTLRMNRSIEEMIRPFPEQWLWLHRRWK"
     gene            complement(2440..>3163)
                     /locus_tag="P5219_03425"
     CDS             complement(2440..>3163)
                     /locus_tag="P5219_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25839.1"
                     /translation="WRSMHTMERAAAAGIRGRLWHLWGTPPSWWGMVRLRARTVHTRF
                     SMKTEWKGHPTVFSATECSGGETYIPPGFEVKVNWGGDAPGEAYRGDESPLCLLPGET
                     SVESDYSSLLGSMGMNFKPLGRGGEEAMQHLSSGRTVLLIENPNSSLSLLSANGALMG
                     VPAAAPRSPLMDEFLGKDGYVHFDPAAGTEEKRRILTLLAGEGGRAASAAARRHVSEQ
                     FAPEKGAKKLENLYLSLSGVKK"
BASE COUNT          690 a          901 c          839 g          733 t
ORIGIN      
        1 cgcatcttac atttatatca tccggggaac gccccttctc atccagctct tcctgattta
       61 tttcggactt ccctccctcg gcctgaatct gccggccttt acggcgggga taatcggcct
      121 ttcgatcaat tcatcaggat acgtgggcga gattgtcagg ggtggaatcg aggcagtgcc
      181 caaggggcaa tgggaggcct ccaaagtgct cggcctttcg tatctgaaga ccatgcgctt
      241 catcatcctt ccccaggcaa tcaggaacat gcttccggcc atcgggaacg agttcgttac
      301 cctcatcaag gaatcttccc tgctgtcaac cctcgcaata acggagctta ccatggtggg
      361 ccagcaggta aggagcgtca cctacgcctc cttcgagacg ttcattgtgg tgggaataat
      421 ttaccttgtt ctgacgagcg tcacgagttt ctccctacaa tacgtggaaa ggaaatggaa
      481 agtcagctga taggttttcc ccaagccgtt aaaaacagga gagaggcatt tcagcctctc
      541 tcctgttttt ttataccatt cttgttccct gggcctctgt ccgctgggcg tatcgggacc
      601 tttccttttc cgccgacaga ccgtcaagat atccccagga agccatgact atctcccgga
      661 tggacagttt cctccgttcc cgcatttcgt tccagaggga aatgggcaga aacatccata
      721 tcctgaaggc tgaatcaggg ccttctccgc cgaaatctga gagaatgaga tatccggact
      781 tgctgtacag cacaacgggc cgggaggact gggagttcat cctctgaagg tcgtcgagtg
      841 aaaaagcaag gccttccatt tcctcgatgt ccctttttcc ggggaggtct gtcagggaga
      901 accatatctt gggcggctgg gttaccccct cgaaaacatc cgcggcactc ttcatccacg
      961 tcttgacccg ggatacggct tcataatcgt tcctgaaaag ggcaagccga atggcccagt
     1021 aggccatctt cgccatgccg tctttttcaa gaacatcaaa aaaggcttct tcgttgtgaa
     1081 gaaactcccc ttctcccaga agatggtctt ctccgatgag ccccctgaga ataagagcgg
     1141 agggcatgtg ctcacccgga ggaggcatct gccccgagag acatgatttc agctgggaaa
     1201 tcacttcgac tgaaatggtc accttctcaa cttctccgag cagaagggtc ttcggtttcc
     1261 cctcgtaaag aaaaacttcc gtggattcat ctgttccaag tttccccagg gtccagcgtt
     1321 cgtaatgttt catggtcgcg gaacccacga gatacctgtt gtaggggaga agaaggagga
     1381 ccctcggaaa cggcccttcg ggcaggccgt caacaatcat gccgtcagga agacgagcga
     1441 tgtttcctcg cgacatgaac tcgaatatca tgaccatccc ctcctcttct gatattttgt
     1501 attagagtag catcaaaaga gcggagtatc aagggaaaaa tacggaacag gactagtttt
     1561 gcaggcttca ttcctttctc tgtccgggaa agaattattt ccatcgcctg tgaagccaga
     1621 gccactgctc gggaaatggg cggatcattt cttcaattga ccggttcatg cggagcgtgt
     1681 tttcatgaat ggcgctgttc ctgtcagtcc cttcggccat gggaagggga ggagaaatga
     1741 ccaccctgtg ccggaagggg gcaagcctgt aagagacaac gggaacgacg ggcactccgg
     1801 caaggtgggc tatggcggcg gggccgccgg ccgtgtagca catccggccg aaaaatgggc
     1861 cgcgcactcc tgaagaatgc catgcctggt cgggaagaag accgagaaat cctccctttt
     1921 tcgcgaggcg aacagcgtcc ttcattatga aatgtttctc tatggtgcct actccgagcg
     1981 ccgcccggta ggtttcaatg agttccgaaa gattcgggtc gtcagggttc cggaccacgg
     2041 ctgttagcgg gtaccccgac tgggcaagcc accctgccag gagttcccag tttcccacgt
     2101 gaccggtcag gatgatcgcc cccctgccgg agtctctgag ttcgtcgaga atttcctttc
     2161 cctcggtttc cacgatccag gaatgcaccc gggagggatc tgagacaagg gagagatatt
     2221 cggccaccat ccaggagatg tgctgataac acttcgtcac tgtctctttt ctccacgatt
     2281 catccgagtc cggaaaggcc agccgcagat tgtcgagggc cactttcctt cttggtctga
     2341 tgaccctgag gccgcaggag aaaaaggatg ccacagccgc agccctccag cccggtctga
     2401 tccatttcct tatgcagtcg agaacggcgc ccctcccttt cattttttga caccggagag
     2461 ggacagatac aggttttcca gctttttcgc acctttttcg ggggcaaatt gttccgacac
     2521 atggcgccgc gctgcagccg aagcggctct ccctccctca ccggcaagca gggtcaaaat
     2581 tcttcgtttt tcctcggtcc ctgcggcagg atcgaaatgg acatatccgt ctttcccgag
     2641 gaattcgtcc atgagcggcg accggggggc cgccgcagga acgcccatga gggctccgtt
     2701 ggcggaaaga agggagagag aggaattcgg gttttctatt agaagaacgg tcctgcctga
     2761 tgacagatgc tgcatggctt cttctcctcc ccggccgagg ggtttgaagt tcattcccat
     2821 acttccgagg agggaagaat agtcactttc tacggacgtc tcccccggca aaagacacag
     2881 aggggactca tcaccccggt aagcttctcc cggagcgtcc cctccccagt tcaccttgac
     2941 ctcgaatccc ggagggatat aggtttctcc cccagagcac tcggtggcgg aaaacactgt
     3001 cggatggcct ttccattccg ttttcatgga aaaccgggtg tgaacggtcc tcgctctgag
     3061 ccgcaccatc ccccaccagg agggaggggt tccccacaga tgccaaagtc ttccccttat
     3121 tcccgcagcc gctgcccgtt ccatggtgtg catggagcgc cat
//
LOCUS       DAOTHD010000142         3158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_36319_length_3158_cov_3.139542, whole genome shotgun sequence.
ACCESSION   DAOTHD010000142 DAOTHD010000000
VERSION     DAOTHD010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36319_length_3158_cov_3.139542"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="P5219_03430"
     CDS             <1..193
                     /locus_tag="P5219_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HRX25840.1"
                     /translation="GSLYGKNIAVFFEEFLRDEEKFDSPEQLVSRMKEDVLLSGRLSG
                     EAARAGNDLYERLGKALRG"
     gene            266..625
                     /locus_tag="P5219_03435"
     CDS             266..625
                     /locus_tag="P5219_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HRX25841.1"
                     /translation="MNFVLKRVYEPCSPEDGTRILVDRLWPRGVSKDGARIDLWLKEI
                     APSPELRKSFCHDPAKWDTFRSMYLEELAGNPVPVGTLLETARHGTVTLLYGAKDPFY
                     NHANVLKEFLDGSSEQR"
     gene            758..833
                     /locus_tag="P5219_03440"
     tRNA            758..833
                     /locus_tag="P5219_03440"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:791..793,aa:Arg,seq:cct)
     gene            876..1379
                     /locus_tag="P5219_03445"
     CDS             876..1379
                     /locus_tag="P5219_03445"
                     /inference="COORDINATES: protein motif:HMM:NF020479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HRX25842.1"
                     /translation="MMKTFSKRILVVFFLSFILFILTVPSYYLKISLEWGEEACCKSL
                     PPGLPFILRFIHSVEQTPVEGEYMVAGGRIRQWEERVRSHNAGLPAEAPLNGRFIPGK
                     DWMAVRGGGASFISLRYRVGDSCSGRNMLIIDGEEIELFRLYPGRILLIRAEKMSFAG
                     RLLSRLF"
     gene            1555..>3158
                     /locus_tag="P5219_03450"
     CDS             1555..>3158
                     /locus_tag="P5219_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRX25843.1"
                     /translation="MNTDYKKTLNLPETDFPMRANLAKREPGFLEFWKERDIFGKMLK
                     NREGAPSFVLHDGPPYANGHIHIGTAFNKILKDFIPKFKAMKGYYAPYVPGWDTHGLP
                     IELHVLKTLKVNKDTVDPVFLREKCTEYALQFRDVQREEFIRLGVLGDWENPYMTLKP
                     QYEAAQLGAFAELVKKGLVYKGQKPVYWCIDCQTALAAAEIEYWDESSPSIFVAYSLP
                     GAGEIHPSLSGQDVNVIVWTTTPWTLPASMAVAMGGDHEYSFFRSGEKVYLFAKNLRE
                     SVEKETGLSFGEEILTVRGRELEGKKAVHPFYPDREILILLADYVLLDTGTGCVHTAP
                     GHGVEDYESGVKYGIEVYNPVDDKGFFRQGTGLVDGLSLKEGSKKILETLTESGRLLG
                     HGAISHSYPHCWRCKKPVIFRSTDQWFVSVSDFRDEALRCIEENVRWIPEWGKERIYN
                     MVRDRSDWCISRQRIWGVPIPAFYCDDCGKPVLTEDRILAVQKHVEKEGCSAWWSKPP
                     EDLLGELCFCPHCGGKHLTKEKDIMDV"
BASE COUNT          688 a          836 c          936 g          698 t
ORIGIN      
        1 tgggtcactg tacggcaaga atattgctgt ttttttcgaa gagttcctcc gggacgagga
       61 aaaattcgat tcgccggagc agctcgtttc cagaatgaaa gaggatgtcc ttctgtccgg
      121 aaggctttcc ggtgaagctg cacgtgcagg gaacgatctt tatgaacggc ttggaaaggc
      181 tcttcggggc tgagacagtc cctgcggtaa aaaagagcgc tccttcggaa gaaccgcagg
      241 tgggaccggg atggaggaaa gagcgatgaa ttttgtgctg aagcgggtgt atgaaccctg
      301 ctctccggaa gacggcacca ggattcttgt agacaggctc tggccccggg gtgtttcgaa
      361 ggatggcgcc cggatcgatc tctggctgaa ggagattgcc ccgagcccgg aactgagaaa
      421 atccttctgc cacgacccgg cgaaatggga tactttcagg agcatgtacc ttgaggagct
      481 cgccggaaat ccggttcccg tgggaacgct cctggagacg gcacggcatg gaacggtgac
      541 cctcctgtac ggggcgaagg atcctttcta caatcatgcc aatgtactga aggaatttct
      601 cgacggctca agcgagcagc ggtgacgttc accgttgcct gcggattcag gtttttccct
      661 tcggcttgaa tttttgccgg agggatggta ctatgtcccc tgttcacgga aacggggtca
      721 aaagagtctc tttccgttcc cgtgaaagaa tatatgcggg cctgtagctc agaggataga
      781 gcgacagcct cctaagctgt aggtcggccg ttcgaatcgg cccaggctcg ccattttccc
      841 cggatcacag gggaagggga aacggagtga agtccatgat gaagaccttt tccaaaagga
      901 ttctcgtggt ctttttttta tccttcatcc tgttcattct gacggttccc tcctactatc
      961 tgaagatttc cctggaatgg ggcgaagagg cctgctgcaa atccctccct cccgggcttc
     1021 cctttattct ccggttcatt cactcggtgg agcagactcc cgtggaaggg gagtacatgg
     1081 tggcgggggg acgcatccgt cagtgggaag agcgcgtccg gtcccataac gccggtctgc
     1141 ccgccgaggc tcccctgaac ggccgtttca ttccgggcaa ggactggatg gcggttcggg
     1201 ggggcggggc gtcttttatc tcgctacggt accgtgtcgg ggacagttgc tcgggcagaa
     1261 atatgctcat catagacggt gaagaaatag aactgttcag actttatccg gggaggatcc
     1321 tgctgattcg ggctgaaaag atgagcttcg ccggcaggct gctgtcacgg cttttctgag
     1381 ttcccttgtt ctccggcacg aagctttctt cgatcccctt atatacggga gagaacgtac
     1441 cggccaaggc ttttgtgggg atttttttct cactatgcta aaatagcctc gacttttatg
     1501 ccgtcctctt ggcacagggg gacggtttcg cctattacac aagggggtag ctgcatgaat
     1561 acggattaca aaaagacgct gaaccttccg gaaaccgatt ttcccatgag ggcgaatctt
     1621 gcgaaaagag aacccggttt tcttgaattc tggaaagagc gggatatttt cggaaagatg
     1681 ctgaaaaaca gggaaggagc accgtcgttt gtccttcatg acggcccccc gtatgccaac
     1741 ggccatattc acatcggaac tgccttcaac aagatactga aggatttcat ccccaagttc
     1801 aaggccatga aggggtatta tgctccctac gttcccggct gggataccca cggtcttccc
     1861 atcgagcttc acgttctcaa gacgctgaag gtgaacaagg ataccgtgga ccccgttttc
     1921 ctgagggaga agtgcacaga gtacgccctc cagttccggg atgtccagag ggaggagttc
     1981 atccgcctgg gcgtcctggg ggactgggag aacccctaca tgaccctcaa gccccagtac
     2041 gaggcggccc agcttggagc cttcgcggag ctggtgaaga aggggctcgt ctacaaggga
     2101 cagaagcccg tttactggtg catcgactgc cagacggccc tcgccgcggc ggaaattgag
     2161 tactgggatg agtcatctcc ctctatcttc gttgcctact cccttcccgg ggcaggggag
     2221 attcacccgt ccctttcggg tcaggacgtg aacgtcatcg tgtggacgac cacgccctgg
     2281 acgctgcccg caagcatggc ggtagccatg ggcggagacc acgagtattc ctttttccgc
     2341 tcgggagaaa aggtgtacct tttcgccaaa aacctccggg agagcgtgga aaaagagaca
     2401 ggactctcct tcggggagga aatactgacg gtgaggggaa gggagctcga agggaagaaa
     2461 gctgtccatc ccttctaccc tgacagggaa atcctcattc tcctcgccga ttacgtcctt
     2521 ctcgacacgg gaacgggctg cgtccacacc gcaccgggtc atggcgtcga ggactatgaa
     2581 tccggcgtga aatacggcat cgaagtctac aatcccgtgg acgacaaggg ctttttcagg
     2641 caggggacgg ggctggtgga cggtctttct ctcaaggaag gatcgaagaa gatcctggag
     2701 actctcacgg agagcggcag gcttctcggc cacggcgcca tttcccattc ttacccccac
     2761 tgctggaggt gcaagaaacc cgttattttc cgctccactg accagtggtt cgtttccgtg
     2821 tctgatttcc gcgatgaagc cctccggtgc atcgaggaaa acgtgcgctg gattcctgaa
     2881 tggggcaagg aaagaatcta caacatggtc cgcgaccggt ccgactggtg catcagcagg
     2941 cagcggatct ggggcgtgcc catccctgcc ttctactgtg acgactgcgg aaagccggtt
     3001 ctcaccgaag accggattct tgccgtgcag aagcacgtgg agaaggaagg atgctccgcc
     3061 tggtggagca agccccctga agacctcctg ggggagctgt gtttctgccc ccactgcggc
     3121 ggaaagcatc tgaccaagga aaaagacatc atggacgt
//
LOCUS       DAOTHD010000143         3139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_36611_length_3139_cov_2.292153, whole genome shotgun sequence.
ACCESSION   DAOTHD010000143 DAOTHD010000000
VERSION     DAOTHD010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36611_length_3139_cov_2.292153"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="P5219_03455"
     CDS             <1..869
                     /locus_tag="P5219_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH dehydrogenase subunit"
                     /protein_id="HRX25844.1"
                     /translation="AFHLGMALREKVMDVLEVLSGNRVNYGVATVGGVRWDVTPELAK
                     AVREMVKYYKKEFSPFYDVVTEDPIARARMRNVGVLTYEEAIRYSAVGPTVRASGVRS
                     DLRESSPYEAYGDFGVRAVVPQDYYDKAYGDVFDRFLVRVHEVYQSLDIIEKVIAGLP
                     EGDIMWEKNLNKVLAFLKKAEGTGWASIEAPRGDDTHVVHLKGGDENVTWWKVRAPTY
                     ANAVSWPLMFRNNELADAPLIINSIDPCISCMERMLATDSSTGARKVVTRAELLEKCR
                     EKTRRLMQNGGR"
     gene            856..1854
                     /locus_tag="P5219_03460"
     CDS             856..1854
                     /locus_tag="P5219_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HRX25845.1"
                     /translation="MVVVSLIMRLIAGAVLMILVAGLAFLFEGVDRVVHARMQRRIGP
                     PVFQPFLDLLKLLGKENIVPRRAVPWAFNGAPWVAAATMLLLFLYIPMGSLPPILGGE
                     GDMILIIYLLGLSGVAMAVGGFASGNPIANVGAQREMILMMSYELPLAVVVSTLAYVA
                     YKTGMPGEPFSLETFAGMSVWGVVGKVGFLGILCLFLALVLVVPGETGKGPMDIPEAK
                     TEILEGLIIEYSGTNLAMFKITFALRATAMAAVIVSIFFPWSLGSFLGFGSVFLYALD
                     FLWFWVKVFVVQIIFVTFMRTAFGRFKIWQASEFYLIKVAGLSIAGMILASIGLVI"
     gene            1871..2521
                     /locus_tag="P5219_03465"
     CDS             1871..2521
                     /locus_tag="P5219_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRX25846.1"
                     /translation="MLNRMALLLLRQVSDKTVTNPFPVQAMPDSLTDALQAAQNGEIE
                     LNPAVETGERFRGRLNYDKSACIGCKLCIKVCPANATEYLPDEKKIQIHNDRCCFCAQ
                     CTEICPVKCLTMSSEYLISSYDRKAQITTDTGKPETKGGAAGEPKKKTVYRIMSDKCI
                     GCTKCARNCPVGCISGKVKEPHVIDASACIGCGKCSEVCPKDAVEAVEIDVPADGK"
     gene            complement(2662..>3139)
                     /locus_tag="P5219_03470"
     CDS             complement(2662..>3139)
                     /locus_tag="P5219_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRX25847.1"
                     /translation="LKRLDPYTRLDLVEPFWREMGLPVDDHSREYLAQSLVVLCGRGQ
                     TAKEAAGYSDYFLTFEAVKGRYDGSDVDDAGRGMLRKFYGELLGLPEWKAPPLEEFTR
                     TWSEGHSVKMKDIAMPLRMALTGMKVSPGVFEVAEHLGRDECRRRLAHFGFVGSAE"
BASE COUNT          652 a          911 c          915 g          661 t
ORIGIN      
        1 ccgccttcca cctgggcatg gcattgcggg aaaaggtcat ggacgtgctc gaggtgcttt
       61 ccggcaaccg ggtgaactat ggtgtggcca ccgtcggagg agtccgctgg gatgtgacgc
      121 ctgaactggc gaaagctgtc agggagatgg tgaaatacta taaaaaggaa ttttctcctt
      181 tttacgatgt cgtcactgaa gaccctatcg caagggcaag gatgaggaat gtcggtgttc
      241 taacctacga ggaagccatc cgctacagtg ccgttggtcc cactgtcagg gcaagcggcg
      301 tcaggagcga cctcagggag agctctccct acgaggccta cggagatttc ggcgttcggg
      361 ccgtggtccc ccaggattat tacgacaagg cctacggcga tgtgttcgac cgttttcttg
      421 tccgtgtcca cgaggtgtac cagtcccttg acataatcga gaaggtcatt gccggcctcc
      481 ccgaaggaga catcatgtgg gaaaagaacc tgaacaaagt tctcgctttc ctcaaaaagg
      541 cagagggcac gggatgggca tccatagaag cgccccgggg cgacgacacc cacgtggtcc
      601 acctcaaggg tggggatgaa aacgtgacct ggtggaaagt ccgggcaccg acctacgcaa
      661 acgcagtatc ctggccactc atgttcagaa acaacgagct tgcggatgca cccctcatca
      721 tcaacagcat cgacccctgc atctcctgca tggaacgtat gctggccact gacagctcca
      781 cgggagcccg gaaagtggtc acccgggccg aactgctgga gaagtgcaga gagaaaacaa
      841 ggaggctgat gcagaatggt ggtcgttagc cttatcatgc gcctcattgc aggcgcggta
      901 ctcatgattc tcgtcgccgg cctggccttc ctcttcgagg gcgtggaccg ggtagtccat
      961 gccaggatgc agcgcaggat cgggcctcct gtctttcagc ccttcctcga cctgctgaaa
     1021 ctgctcggga aagagaacat cgttccccgc agggccgtcc cgtgggcctt caacggtgct
     1081 ccgtgggtgg ccgccgccac catgctgctc ctgttcctct acattcccat gggttccctg
     1141 cctcctattc tgggcggtga aggggacatg atcctcatca tctaccttct cggtctctcc
     1201 ggcgttgcca tggccgtagg cggcttcgcc agcggtaacc ccatcgcaaa cgtgggcgcc
     1261 cagcgggaaa tgatcctgat gatgagctac gagctccccc ttgctgtagt ggtgagcact
     1321 ctggcctatg tcgcctacaa gaccggcatg ccgggagaac ccttcagcct ggaaactttc
     1381 gccggaatgt cggtgtgggg tgttgtgggc aaagtgggat tccttggcat tctctgcctc
     1441 ttccttgccc ttgtccttgt ggttcccgga gagaccggca aggggcccat ggacatcccc
     1501 gaagcgaaaa cggaaatact ggaaggactc atcatcgagt attccggaac aaacctcgcc
     1561 atgttcaaga tcaccttcgc cctgagagcc acggccatgg cggccgtcat cgtgtccatt
     1621 ttcttcccgt ggtctctcgg ctctttcctc ggcttcggga gcgttttcct ctatgccctg
     1681 gatttcctgt ggttttgggt gaaggttttc gtagtccaga tcatctttgt caccttcatg
     1741 cggaccgcct tcggccggtt caaaatctgg caggcgtccg aattctacct gatcaaggtt
     1801 gcgggtcttt ccatcgccgg aatgatactt gcatccatcg gtctggtgat ctagaaaggg
     1861 ggaggaaaga atgctgaacc ggatggccct gcttctgctc cggcaggtta gtgacaagac
     1921 ggtgacaaac cccttccccg tgcaggctat gcccgattcc cttacggacg cccttcaggc
     1981 cgcccagaac ggagaaattg aactcaaccc ggcggttgaa acgggggaga ggtttcgagg
     2041 caggctgaat tacgacaagt ccgcatgcat cggatgcaag ctctgcatca aggtgtgccc
     2101 tgcgaacgcg acggaatacc tgccggatga gaagaagatc cagatccaca acgaccgctg
     2161 ctgcttctgt gcccagtgca ccgagatctg cccggtgaag tgcctcacca tgtcgtcgga
     2221 ataccttatc tcctcctacg acaggaaggc ccagatcact acggatacag gcaagcccga
     2281 gacaaagggc ggtgccgccg gcgagccgaa gaagaagacg gtctacagga ttatgtcgga
     2341 caagtgcatc ggctgcacca agtgcgccag gaactgcccc gtgggctgca tttccggaaa
     2401 ggtcaaagag ccccacgtca tcgatgcatc ggcttgcatc gggtgcggaa aatgttcgga
     2461 agtctgcccg aaggatgcag tggaggccgt ggaaatcgac gttcctgcgg acggaaagta
     2521 atctgcctgc gggaccggaa ggaaaaagcg aacggaccgg gaaatgtccc ggtccgttcg
     2581 ttcatttagg acctgtcgga atcaagaaca gagtctttct ttgccgaagg ggtgatgcct
     2641 gtttcccctt tcggagctac ttcactccgc cgatccgaca aagccgaaat gggcgaggcg
     2701 cctccggcat tcgtccctgc cgaggtgttc cgccacctcg aagacgccgg ggctgacttt
     2761 catgcctgtg agggccatcc gcaggggcat ggcgatgtcc ttcattttga cggaatgccc
     2821 ttcgctccac gtgcgggtaa actcttccag gggaggcgct ttccattcgg gaaggccgag
     2881 gagttccccg tagaatttcc ggagcatccc ccttcccgcg tcgtccacgt cagaaccgtc
     2941 gtacctgccc ttcacagctt cgaaggtgag gaaataatcc gagtaccccg cagcctcctt
     3001 cgccgtctgc ccccttccgc agaggaccac gagtgactgg gcgaggtatt ctcgtgaatg
     3061 atcgtccacg ggaagcccca tttccctcca gaagggttcc acaaggtcga gcctggtata
     3121 gggatcgagg cgcttcagg
//
LOCUS       DAOTHD010000144         3138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_36622_length_3138_cov_3.330847, whole genome shotgun sequence.
ACCESSION   DAOTHD010000144 DAOTHD010000000
VERSION     DAOTHD010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36622_length_3138_cov_3.330847"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="P5219_03475"
     CDS             complement(<1..636)
                     /locus_tag="P5219_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRX25848.1"
                     /translation="MSIIVSGLGHTYHPGTPLETHALRDISFEFPAGTWFSVVGHTGS
                     GKSTLAQHLNGILLPMEGEIFIDGLEVKEKSPSLRDIRRKVGLVFQYPEQQLFAETVS
                     DELAFAPRNWGVPEQDIPGRVAAAAAQVGLDEGCLGRSPFHLSGGEKRKAAIASVLSS
                     LPDYLVLDEPTAGLDSFSRKELTRLLSGLRKEGMGILLVTHDLDIALQMSDR"
     gene            complement(627..1472)
                     /locus_tag="P5219_03480"
     CDS             complement(627..1472)
                     /locus_tag="P5219_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRX25849.1"
                     /translation="MPRKTVCELRDVTFVYPGGAAGGSGCASPALRNVTLSIYEGEWV
                     ALLGSNGSGKSTLAKHLNALLVPSQGDCLVYGLSTRDEKNVFPIRSTVAMVFQNPENQ
                     IVGTVVEEDAAFGPENQGVPPDEIVQRVEVALAVAGLLEKRKKPTYTLSGGEKQRLAV
                     AGALAMDTPCLVLDEPTAMLDPAGRAELLRVLRNLHASGKTIVSITHRLEEILSCDRA
                     IILHRGEIAWEGTVSALFLSDLPFKEWGLDIPPLVSFWRDLRAEGRSVPPVPTVAAMA
                     DALCQ"
     gene            complement(1475..1828)
                     /gene="rplQ"
                     /locus_tag="P5219_03485"
     CDS             complement(1475..1828)
                     /gene="rplQ"
                     /locus_tag="P5219_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRX25850.1"
                     /translation="MRHRMDSRTLGRYGSHRRAMLSNMAASLFLEESITTTVTRAKEL
                     RRYAERLITRAKGGTIHDRRIVRSKMAHKEAAIKLFEDLAKRYASRPGGYTRIIKTGN
                     RHGDGSPMAVIALVD"
     gene            complement(1834..2826)
                     /locus_tag="P5219_03490"
     CDS             complement(1834..2826)
                     /locus_tag="P5219_03490"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRX25851.1"
                     /translation="MRPEIRIEESTATFAKVHIEPLERGYGATLGNSLRRVLLSSIRG
                     AAISSVRIDGVLHEFSTIPGVREDVIEILLNLKHVPVRSFSSEVRVLHLEAEGAGTVT
                     AADIQPDSEVEFVRPDAPICTMEEGGRLSMQIYVEQGVGYASMERPRPPYLPVDALMV
                     DAIFSPVLKVNYDVEAARVGQRTDYERLVLQVWTNGVHSPESTVCEASKILRGYFSNF
                     VEDMEKKHPDERAEQDTPVSHGGAEAAAERSGEEDDYLARPVRDLELSIRSENCLLRG
                     GIHTIGDLMSKTREDLLKIRNLGKISLREIEEKMEKLGAVISSEKGETKSTKED"
     gene            complement(2859..>3138)
                     /locus_tag="P5219_03495"
     CDS             complement(2859..>3138)
                     /locus_tag="P5219_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HRX25852.1"
                     /translation="RQLVLHGHFLVNGKRVDIPSFMVSAGDVVAAKEKSRETALLKEN
                     AEVAAARTVPQWLEVNAEKIEGKVIALPARDQIDAPVTEQLVVEFYAR"
BASE COUNT          547 a          914 c         1012 g          665 t
ORIGIN      
        1 gcggtcgctc atctggagag cgatgtcaag gtcgtgggtg accagcagga tgcccattcc
       61 ctccttccgg aggccggaca gcaggcgggt gagttccttc cgggaaaagg agtcgaggcc
      121 ggcggtgggc tcgtcgagaa cgaggtagtc aggcagggag gacaggacgg aagcgatggc
      181 ggccttccgc ttttctcccc ctgaaaggtg gaaggggctt cttccgaggc atccctcgtc
      241 aagtcccacc tgggcggcgg cggcggcgac ccgccccgga atgtcctgct cgggaacgcc
      301 ccagttccgg ggagcgaagg cgagttcgtc actgaccgtt tcggcgaaga gctgctgttc
      361 ggggtactgg aacaccaggc cgaccttccg gcggatgtcc cggaggctcg ggcttttttc
      421 cttcacttcc aggccgtcga tgaaaatctc cccctccatg ggaaggagaa ttccgttgag
      481 gtgctgggcg agggtcgact tgccgcttcc cgtatggccc accacggaga accatgtgcc
      541 tgcgggaaac tcgaaggaga tgtcccggag agcgtgggtt tcaagggggg ttcccgggtg
      601 gtaggtatgg cccaggcccg aaacgattat tgacatagcg cgtcggccat ggccgccacc
      661 gtcggcacgg ggggcacgga acgcccctcc gccctgaggt cacgccagaa ggacacaaga
      721 gggggtatgt cgagccccca ctctttgaag ggaaggtcgg agagaaaaag ggcggaaacg
      781 gttccctccc atgcaatctc tcccctgtga aggataatcg cccggtcgca ggaaagaatc
      841 tcctccagcc ggtgggtgat ggagacgatg gtcttgccgg aggcgtgaag gttccggagt
      901 acccggagga gttccgcccg gccggcgggg tcgagcatgg ccgtgggttc gtcgaggacg
      961 aggcacgggg tatccatggc gagggcccct gccacggcga ggcgctgttt ttctcctccc
     1021 gagagggtgt aggtgggttt ttttcttttt tcgagcagtc cggccacggc aagggcaacc
     1081 tccacccgct ggacgatttc atccgggggg acgccctggt tttccggtcc gaaggccgcg
     1141 tcttcctcca ccacggttcc gactatctgg ttctcggggt tctgaaagac catggcgacg
     1201 gtgctccgta tagggaagac gtttttctca tcccgggtgg aaaggccata cacaagacaa
     1261 tcgccctgcg agggtaccag cagggcgttc aggtgctttg caagggttga tttgcctgat
     1321 ccgttgcttc cgagaagcgc cacccactcc ccctcgtata tggaaagcgt cacgttccgc
     1381 agggcggggc tggcgcagcc ggagccgccg gccgctcctc cgggatagac gaaagtcaca
     1441 tcccggagtt cgcataccgt ttttcggggc ataatcagtc gaccagggct atgaccgcca
     1501 tgggggagcc gtctccgtga cggttccctg tcttgatgat gcgggtatat ccgcccggac
     1561 ggcttgcata ccgcttcgca aggtcttcga aaagcttgat ggcggcttcc ttgtgggcca
     1621 tcttggatcg gacgatgcgc cggtcgtgga tggtgccgcc cttggcccgg gtgatgagcc
     1681 gctcggcgta ccggcgaagc tccttggccc gggtgacggt ggtggtgatg ctttcttcaa
     1741 ggaaaaggct ggccgccatg ttggacagca tggcccttct gtggctgcca taccggccga
     1801 gcgtccggct atccatacga tgtctcatgg aaattaatcc tccttagttg actttgtttc
     1861 gcccttttcg ctcgagatga cggctccgag cttttccatt ttctcttcga tctcccggag
     1921 ggaaatcttg ccgaggttgc ggatcttcag caggtcttcc ctggttttgc tcatgaggtc
     1981 gcctatggtg tggattccgc ccctgagcag gcagttttcg ctgcggatgg agagttcgag
     2041 atcacggacg ggacgggcga ggtaatcatc ctcttcgccg gaccgttccg cggccgcctc
     2101 ggcaccgccg tgggaaaccg gcgtgtcctg ttcggccctt tcgtcggggt gtttcttctc
     2161 catgtcctcc acgaagttcg agaagtatcc tctcaggatc ttcgaggctt cgcacacggt
     2221 ggactccggc gaatggacgc cgttggtcca gacctggagc acgagccgct cgtagtcggt
     2281 ccgctggccg acccgggctg cctccacatc gtaattcacc ttcaggacgg gcgaaaagat
     2341 ggcgtcaacc atgagggcgt ccacgggaag gtaggggggg cggggccgtt ccattgaagc
     2401 gtaccccacg ccctgttcaa cgtagatctg catggaaagc ctgccgcctt cctccatggt
     2461 gcagatgggt gcgtcaggcc tgacgaattc cacttcgctg tcgggctgga tgtcggcggc
     2521 cgtcacggtg cccgcgcctt ccgcttccag gtggagaacc cgtacttcac tgctgaagga
     2581 gcgcaccggg acatgcttaa ggttcagcag gatctcgatg acatcttccc ggactccggg
     2641 gatggtgctg aactcatgga gcacgccgtc tatgcggacg gacgatatgg ccgcacccct
     2701 gatcgaggaa aggagaaccc tgcggagcga attgccaagg gtagctccat atccacgctc
     2761 aaggggttca atatgaacct tcgcgaaggt ggcagtgctt tcctcgattc ggatttcagg
     2821 ccgcatatgt tccaaggttt ccaacacctt tcacgccatt atctggcata aaactcgacg
     2881 acgagctgtt cggtcacggg cgcatcaatc tggtcacggg cgggaagggc gatgactttt
     2941 ccttcgatct tttcggcgtt gacctcgagc cactggggca cggtacgtgc ggcggcgact
     3001 tccgcgtttt ctttcagaag ggccgtttcc ctgctcttct ccttggcggc gaccacgtca
     3061 ccggcgctga ccatgaagct ggggatgtcc acccttttgc cgttcacgag gaagtgaccg
     3121 tgaaggacga gctgcctg
//
LOCUS       DAOTHD010000145         3138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_36629_length_3138_cov_2.767434, whole genome shotgun sequence.
ACCESSION   DAOTHD010000145 DAOTHD010000000
VERSION     DAOTHD010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36629_length_3138_cov_2.767434"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="P5219_03500"
     CDS             <1..953
                     /locus_tag="P5219_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRX25853.1"
                     /translation="PSFLCERAEDMDRFLDTQLRKLCTGTVDYYLLHTLTGPLWDHLE
                     SLGARKFLDRAKSDGRIVNAGFSFHGASEDFRRIVDAYPWEFCQIQYNFLDEEYQAGT
                     KGLEYAASKGLGVVVMEPLRGGNLGLPVPPPAIAELWKRAPLVRTPAEWALRWVWNHP
                     EVTVVLSGMNEEKHIEENLRIAGAALPGSLTAEELGLVGEAAAKYKELMKVGCTGCGY
                     CMPCPAGVAIPACFEVYNKMHLFGNVQEALFMYAMRMSGILRKGAPNFASQCVQCGQC
                     LEKCPQGLAIPDLLKQVAEELETPGLEERVAKARKMLNLD"
     gene            complement(1018..1311)
                     /locus_tag="P5219_03505"
     CDS             complement(1018..1311)
                     /locus_tag="P5219_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRX25854.1"
                     /translation="MEDKSKDIIVCRCEEVTMGEIREWIAKGYDTFDELKRLLRVGMG
                     PCQGRGCREIIMREIAKATGRRMADVDPGIFRPPVKPVKLGVLATCEYSKEEK"
     gene            complement(1314..1820)
                     /locus_tag="P5219_03510"
     CDS             complement(1314..1820)
                     /locus_tag="P5219_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRX25855.1"
                     /translation="MEMDMLFNSGILTSERPGAVKPPEELWEKKTNGLVIIECPQRIP
                     CNPCHTSCPTGAVLPFEDINDTPRIDYAKCTGCAMCVAKCPGLACFVVDLTYGGPDEA
                     LMKLPYELLPVPAAGETVDCLNRAGEVVAQGKVVRVMEPWKDKTLVVHVSVPKALVMD
                     IRAIKVVK"
     gene            complement(1825..2925)
                     /locus_tag="P5219_03515"
     CDS             complement(1825..2925)
                     /locus_tag="P5219_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRX25856.1"
                     /translation="MRQTEVMVIGGGPAGLSAAVEAASLGCQVMVVDSDLKLGGQLIK
                     QTHKFFGSKEEHAGTRGFQIADILFKELAENKDRIQVLSNTTAVGYYKEDGVVSCVTG
                     EDEYFRVKPQRVIMATGAQEKMIPFPNNDLPGIYGAGAVQTLMNVYGVVPGKRVLMVG
                     AGNIGLIVSYQMKQAGVDIAGIVEFMPHVGGYWVHAAKDRRLGIPIYLRHTILEALGD
                     RVVTGALVQEVGEKGGLVGEPKKIDCDVICMAVGLSPTCELLWQAGCEMTFVPELCGY
                     VAKRDNQMRTSVPTIWVAGDAAGIEEASSAMVEGRVAGLNAARSTGHVTDSEFDARSS
                     LYWKRLSDLRAGPVGEKIRYGISQACCGSWEV"
     gene            complement(2944..>3138)
                     /locus_tag="P5219_03520"
     CDS             complement(2944..>3138)
                     /locus_tag="P5219_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRX25857.1"
                     /translation="NGVYIYRETPEMKRPRGFFCAIGKCSSCFMVLDGVPNVRTCVTA
                     LVEGMKVETQRGKGLVPALS"
BASE COUNT          563 a         1132 c          843 g          600 t
ORIGIN      
        1 tgccttcctt tctctgcgaa agagcggagg acatggaccg gttcctggac acccagcttc
       61 ggaaactttg cactggaaca gtcgattact acctcctcca caccctgacc ggccccctct
      121 gggaccactt ggagtccctt ggcgccagga aattcctgga cagggcaaaa tcggacggcc
      181 ggatcgtcaa tgccgggttt tccttccatg gtgcctcgga ggatttcagg aggatcgtgg
      241 acgcctaccc ctgggagttc tgccagatcc agtacaactt cctggacgaa gaataccagg
      301 caggaaccaa aggcctcgag tacgccgctt cgaaggggct gggagtggtc gtcatggaac
      361 cgctcagggg cggcaacctg gggctgccgg taccccctcc tgccatagcc gaattatgga
      421 aaagggcacc gctcgtccgg actcccgcgg aatgggccct gcgttgggtc tggaatcacc
      481 cggaggtcac cgtggtgctc tccggcatga acgaagaaaa gcatatcgaa gagaatttga
      541 ggatagcggg agcggccctg cccggttcgc ttaccgctga agaactggga cttgtcgggg
      601 aggcggcggc gaaatataag gaactcatga aagtcggctg cacgggttgc ggctactgca
      661 tgccctgccc ggcaggggtc gccatcccgg cctgcttcga ggtgtacaac aagatgcacc
      721 ttttcggcaa cgtccaggaa gccctcttca tgtatgccat gagaatgagc ggaattctgc
      781 ggaaaggggc tcccaatttc gcctcccagt gtgtccaatg cggccagtgc ctcgagaaat
      841 gcccccaggg cctggctatc cccgacctcc tgaaacaggt cgcggaggaa ctggaaaccc
      901 ccggactcga agaacgggtg gccaaggccc ggaagatgct caacctggac tgaaagtcga
      961 tgaaaagagg ggcggcgccg tttccggcgc cgcccctctt ttcatcgggc aatagcctta
     1021 tttctcttcc ttcgagtact cgcaggttgc gaggacgccg agcttgaccg gcttcactgg
     1081 gggccggaag atcccggggt ccacgtcggc catgcggcgg cccgtggcct tggcgatctc
     1141 gcgcatgatg atctcgcggc aaccgcgccc ctggcacgga cccattccca cccgcaggag
     1201 acgcttcagt tcgtcgaagg tgtcgtaacc cttcgcgatc cactccctga tctcgcccat
     1261 ggtcacttcc tcgcagcggc agacgatgat gtccttgctc ttgtcttcca tgcctacttc
     1321 accaccttga tggcacggat gtccatcacc agcgccttcg gcaccgacac gtggacaacc
     1381 agcgtcttgt ctttccacgg ctccatcacc cgcacgacct tgccctgcgc caccacttct
     1441 ccggcacggt tcaggcagtc caccgtctcc cccgctgccg gtaccggcag aagctcgtac
     1501 ggcagcttca tcagcgcctc gtcaggaccg ccgtacgtca ggtccaccac gaagcacgcc
     1561 agacccgggc acttcgccac gcacatcgcg cagcccgtgc acttcgcgta gtcgatccgc
     1621 ggcgtgtcgt tgatgtcctc gaacggcagc accgctcccg tcgggcagct cgtgtggcaa
     1681 gggttgcacg ggatccgctg cgggcactcg atgatcacca gcccgttcgt cttcttctcc
     1741 cacaactcct ccggcggctt caccgcaccg ggtcgctccg acgtcaggat cccgctgttg
     1801 aacagcatgt ccatttccat ctgactacac ctcccagctg ccgcagcaag cctgggagat
     1861 cccgtagcgg atcttctccc ccacgggccc cgccctcagg tcgctcagcc gcttccagta
     1921 cagcgacgaa cgcgcgtcga actcgctgtc cgtcacgtgg cccgtcgaac gcgccgcgtt
     1981 cagtcccgcc acccggcctt ccaccatcgc cgagctcgct tcctcgatac ccgccgcgtc
     2041 gcccgccacc cagatcgtcg gcacgctcgt ccgcatctgg ttgtcgcgct tcgccacgta
     2101 tccgcaaagc tccggcacga acgtcatctc gcagcccgcc tgccacagaa gctcgcacgt
     2161 cggcgacagc cccaccgcca tgcagatcac gtcgcagtcg atcttcttcg gttcgcccac
     2221 caggccgccc ttctctccca cttcctggac cagcgcccca gtcaccacac ggtcgccaag
     2281 cgcttccagg atcgtgtgcc tgaggtagat ggggatccca aggcgacggt ccttcgccgc
     2341 atgcacccag tagccaccca cgtgcggcat gaactccacg attcccgcga tgtccactcc
     2401 cgcctgcttc atctggtagc tcacgataag gccgatgttc cccgctccca ccatcaggac
     2461 ccgcttgccc ggcaccacgc cgtacacgtt catcagcgtc tgcaccgcgc ccgctccgta
     2521 gatccccggc aggtcgttgt tcgggaacgg aatcatcttc tcctgcgctc ccgtcgccat
     2581 gatcacgcgc tgcggcttca cacggaagta ctcgtcctct cccgtcacgc aggagaccac
     2641 gccgtcttcc ttgtagtagc ccaccgccgt cgtgttcgac agcacctgga tccggtcctt
     2701 gttctccgcc agttccttga acaggatgtc cgcgatctgg aatccacgcg tccccgcgtg
     2761 ctcctccttc gaaccgaaga acttgtgcgt ctgcttgatc agctggcccc caagcttcag
     2821 gtcgctgtcc accaccatca cctggcaacc caggctcgcc gcttccactg ccgcagaaag
     2881 acccgcaggc cctcctccga tgaccatcac ttccgtctga cgcaacgcgc tcaccttcct
     2941 cccctaggac agtgccggca ctagaccctt gccgcgctgc gtctcgacct tcatgccctc
     3001 aaccagggcg gtgacgcagg tccgtacatt cggcacgccg tccaggacca tgaagcacga
     3061 actgcacttc ccgatggcgc agaaaaatcc ccggggacgc ttcatctccg gcgtctctcg
     3121 gtagatgtac accccgtt
//
LOCUS       DAOTHD010000146         3118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_36923_length_3118_cov_2.965393, whole genome shotgun sequence.
ACCESSION   DAOTHD010000146 DAOTHD010000000
VERSION     DAOTHD010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36923_length_3118_cov_2.965393"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="P5219_03525"
     CDS             complement(<1..285)
                     /locus_tag="P5219_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M20"
                     /protein_id="HRX25858.1"
                     /translation="MLSEKIDAMKDELVAAIQDVVRIPSVGGEPAEGAPYGEGPRKCL
                     DWTLAFAEKMGFRTKNVDNVAGWAEMGEGDEMVAVLGHLDVVPEGKGWTVD"
     gene            complement(310..1677)
                     /locus_tag="P5219_03530"
     CDS             complement(310..1677)
                     /locus_tag="P5219_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRX25859.1"
                     /translation="MAKERVLIVGGDAAGMSAAGQIRKLLPEADIVVYERSGYSSYSA
                     CGIPYFVAGLVEPETRLVVRTPEEFLEKQNISVHTRHEVLSLDPDGGTLLVRDLESGR
                     EFRDSWDKLLLATGASPAVPPVEGAEAEGIFTLSTLETGIAARRYVDEVRPRHAVVIG
                     GGYIGLEMAEAFACVRGMKVTLVDKSPQVMNTFDADMAELIASAIRGIGTELRLGEGL
                     VGFGTQGGRVRSVITEHGEIETEMVIMGLGVRPNSGLAGAAGLKLSVRDSILAGETMA
                     TSRPGIWSAGDCASTTHLLTGKPFWIALGTVANKMGRVAGISMGGGRASFPGVYGTAM
                     SKHCGLEVARTGLTEREAEENGFRYVSSVIRTKTRAGYYPGAETMHVKLVGEEETGRL
                     LGGQIVGGQGSAKRVDIIATALAGGMTVNDLLELDLGYAPPFSMAWDPVQIAGRELLK
                     KTGGE"
     gene            complement(1755..3014)
                     /locus_tag="P5219_03535"
     CDS             complement(1755..3014)
                     /locus_tag="P5219_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRX25860.1"
                     /translation="MPDWEKSTLFRAFRHRNFRLFYMGQIVSLSGLWMQRVAMNWLVY
                     RITGSPLLRGTVDFMTQIPVLLFGSLTGAFLEGRNLRRVIMICQTISMLHALTLAVLT
                     LSGVVEYWHILILAAVIGISDSFEVPARQGFTIRLVEDEKDLGNGIALTSTLFNVTRL
                     VGPSIAGMVIAVVGEGICFLMNGMAYSATLTALFLMRFRKPLVTDQVRKSVLEGMKEG
                     VRYVASFLPLRNYLGAIALVSFFAFPYMVLLPVFAREILGGGPRTLGFLMGATGLGAL
                     AGSVRLALRKSPVGLGRIMAVSCILLGFSMAAFSLSRMFSLSLVLMACLGFFMVSTLV
                     SCNTLVQTLVDEDKRNRVMSLFIVCAMGITPIGSLNAGWLATHIGAPATLFAGGTISL
                     GIGLLLLRAWPSMWALSEPVYRRKNLL"
BASE COUNT          575 a         1002 c          993 g          548 t
ORIGIN      
        1 gtccaccgtc cagccctttc cctcgggaac cacgtccagg tgtccgagga cggccaccat
       61 ctcgtcccct tcgcccatct cggcccagcc tgccacgttg tccacgttct tcgtccggaa
      121 gcccattttc tccgcgaagg cgagggtcca gtcgaggcat ttcctcggcc cctccccgta
      181 gggggctcct tcagcgggct cgccgccgac gctggggata cgcacaacat cctgtatggc
      241 ggccacaagt tcatctttca ttgcatcgat tttttccgac agcacgaaat tacctccttg
      301 atgggcgggt cattccccgc ctgttttttt cagcagttcc cttccggcga tctggaccgg
      361 atcccatgcc atggagaagg ggggagcata tccgaggtca agctccagga gatcgttcac
      421 cgtcatgccc cccgcgaggg ccgtggcgat gatgtccacc cgcttcgccg aaccctggcc
      481 gcccacgatc tgcccgccca gcagcctgcc cgtttcttcc tcgcccacga gcttcacgtg
      541 catggtctcc gccccggggt aataccccgc ccgggtcttg gtccggatga ccgaggagac
      601 gtaccggaag ccgttttctt ccgcttccct ctccgtcagg ccggtccggg cgacttcgag
      661 gccgcagtgc ttgctcatgg cggtgccata gacgcccggg aaagaggccc ggccgccgcc
      721 catggagatc cctgccaccc gccccatctt gttggccacg gtgcccaggg cgatccagaa
      781 gggtttcccc gtcaggaggt gggtggtgga ggcgcagtct ccggcggacc agattccggg
      841 acgggatgtg gccatggtct cgccggcgag gatggaatcc cgcacggaca gcttcagccc
      901 cgcagccccc gcgagccctg agttggggcg gacgccgagg cccatgatca ccatttccgt
      961 ttcgatttcc ccgtgttcgg tgatgaccga ccggacccgg ccaccttggg tgccgaagcc
     1021 aaccaggcct tcgccgaggc ggagttccgt cccgatcccg cggatggcgg aggcgatgag
     1081 ttccgccatg tcggcgtcga aggtgttcat gacctgggga gacttgtcca cgagggtcac
     1141 cttcatcccc cggacacagg cgaaggcctc ggccatctcc aggccgatat agcctccccc
     1201 gatgaccacg gcgtgccggg gccggacttc gtccacgtag cgccgggcgg cgatgccggt
     1261 ctccagggta ctgagggtga agataccctc cgcttccgca ccctcaacgg gaggcaccgc
     1321 cgggctcgcc cccgtggcga gaagcagctt gtcccaggag tcccggaatt cccttcccga
     1381 ctcgaggtcc ctcaccagca gggtcccccc gtcaggatcg agagaaagca cctcgtggcg
     1441 ggtatggacc gagatattct gcttctccag gaattcctcc ggcgtccgga ccacaagcct
     1501 ggtttcgggc tccacgagcc cggcgacaaa gtagggtatg ccgcacgcgg aataggaaga
     1561 gtagccgctc cgctcataga cgacgatgtc cgcttcggga agcagtttcc ggatctgtcc
     1621 ggcggcggac atgcccgcag cgtcgccgcc cactatgaga acccgttctt tcgccaattc
     1681 cttcacctcc cgggaatgcc tgtatcaggt attttttctc ccctattata cccgagagtt
     1741 tcggacggag ggagctacag gagattcttc cgccggtaga ccggttcgga gagcgcccac
     1801 atggacggcc acgcccgaag gaggagcagg ccgatgccga ggctgatcgt cccgccggcg
     1861 aagagggtgg cgggagcgcc gatgtgggtg gccagccatc ccgcgttcag gctcccgatg
     1921 ggggtgatgc ccatggcaca gacgatgaaa aggctcatca ccctgttccg cttgtcctcg
     1981 tccaccaggg tctggaccag ggtgttgcag gagaccaggg tagagaccat gaagaatccg
     2041 aggcaggcca tgaggaccag ggaaagagag aacatcctcg agagggagaa ggccgccatg
     2101 gagaacccga ggaggatgca ggacaccgcc atgatcctgc cgagacccac gggggatttt
     2161 ctcagggcga gccggacgga ccccgccagg gcgcccagcc ctgtggctcc catgaggaag
     2221 cccagggtgc gggggcctcc gccgagaatt tcccgggcaa agacgggaag aaggaccatg
     2281 taggggaagg cgaagaagct gaccagggcg atggcaccga ggtaattccg cagggggagg
     2341 aaggaggcaa cgtaccggac accttccttc atgccttcga ggacgctctt ccggacctgg
     2401 tcggtgacga ggggcttcct gaagcgcatc aggaagaggg cggtgagggt ggcggaatag
     2461 gccatgccgt tcataaggaa gcatatcccc tcccccacca cggcgatgac catgcccgcg
     2521 atggagggtc ccacaagcct tgtgacgttg aagagggtgg atgtgagggc gatgccgttg
     2581 cccaggtcct tctcgtcctc cacgaggcgg atggtgaaac cctgccgggc ggggacctcg
     2641 aaggagtccg agatgccgat gacggccgcc aggatgagga tatgccagta ttccactact
     2701 ccagacaagg tcaggacggc aagggtgagg gcgtggagca tgctgatggt ctgacagatc
     2761 atgatgaccc tccgcaggtt ccgcccctcg aggaaggcgc cggtgaggga cccgaaaagc
     2821 agcacgggaa tctgggtcat gaagtccacc gtcccgcgca gaagaggcga gccggtgatc
     2881 ctgtagacga gccagttcat ggccacccgc tgcatccaga gtcccgagag ggagacgatc
     2941 tgccccatat agaacagccg gaagttcctg tgccggaagg cccggaagag ggtgcttttt
     3001 tcccagtcag gcatggcggc ggcttatcct ttccagaccg cgcggtactt cttgaggaac
     3061 cggaccgggt tgtaggactc cttggcctgc cggacgcctt cctctcccat gtcctgct
//
LOCUS       DAOTHD010000147         3110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_37058_length_3110_cov_3.196727, whole genome shotgun sequence.
ACCESSION   DAOTHD010000147 DAOTHD010000000
VERSION     DAOTHD010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37058_length_3110_cov_3.196727"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="P5219_03540"
     CDS             complement(<1..535)
                     /locus_tag="P5219_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25861.1"
                     /translation="MLKNVKIGSKLAAGFAVVLVIFSAAGWFAWRNMNTVISEVGTLE
                     QRYVPGIELSNEIENIAQTLMLHIRTYVLADSFQDLQAAREDFEAMAEVLKKAGEHAS
                     VHPGLELLAEGVKKSEDQMASYKALLEKSQGLIAAMAGQKRKAAEAGESMRKIAYDLL
                     YDEKDALEKDLNDPASRG"
     gene            complement(703..1809)
                     /locus_tag="P5219_03545"
     CDS             complement(703..1809)
                     /locus_tag="P5219_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRX25862.1"
                     /translation="MKVRDFLLERYFARYEFSVRHLLSPSDCESLRAEELLEGSGPGI
                     AELWRELRLTYTESRGHPLLREEIAAASGVTPGDTLVLNPEEGIFLTMNALLDEKDHV
                     IVLSPGYQSLYEIPKSLGCEVTRWYLKKENGAWKLDLPFLQEAAKENTRLIVINFPHN
                     PTGFLPTEKELSAVVDFASKKGIFLFCDEMYRGLEHDPEKRLPSVPSLYRKSAALGGL
                     SKTYGLPGLRIGWITSKDEWLLDRVASLKDYTTICSSAPSEILAIAALRRRDELAERC
                     RAIVGRNLALAREFFSGRKDRFEWFEPEGGSTAFPKLLGSQPVRDFCERAVEEKSLMV
                     LPDFVFDVDWNHFRIGLGRENFPEALEVLGNFLG"
     gene            complement(2004..>3110)
                     /locus_tag="P5219_03550"
     CDS             complement(2004..>3110)
                     /locus_tag="P5219_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRX25863.1"
                     /translation="AEKMELVAAGDLTSTVELRSSLAEVKLLVDGINSMITLVAGSMR
                     DISKSSRAVLARAEDMSAAAEESTASIEEVMAMAEKASSNTESAAASVEETNAGVEEV
                     AAGAQAGAKAAVEAGEEAALISDAARKGGKAVEDMAGLITETAKAGEDVSSAVTNLAG
                     TVKNISGFVSIITSIADQTNLLALNAAIEAARAGEAGRGFAVVAEEVRKLAEESNRAA
                     GEVGKLIGEISSRTDSALRDSANSSKILKDLVVRADETSILIADVVKRVNNVAENIQT
                     IAATMEEQSASAEEMTAGMDSVAKSSSEIADQVSGIAGAMQEQSKVVESIASASEELV
                     NLSAAMEHAVSRFRTGEENRGLVPETSVSVKGKK"
BASE COUNT          574 a          990 c          878 g          668 t
ORIGIN      
        1 tgccccggga tgcggggtcg ttcaggtctt tttcgagggc gtccttttcg tcatagagca
       61 gatcgtaggc gatttttctc atggactccc ccgcctcggc ggccttccgc ttctggccgg
      121 ccatggcggc gatgagtccc tgggatttct ccaggagggc cttgtaggat gccatctggt
      181 cctctgactt tttcacgcct tcggccagaa gctcaagacc cggatggacc gacgcatgtt
      241 ctcccgcctt tttcagcacc tccgccatgg cctcgaaatc ctcccgggcc gcctggaggt
      301 cctggaagct gtccgcgaga acgtatgtgc ggatgtggag catcagggtc tgggctatgt
      361 tctcgatttc gttggaaagc tcgatgcccg gaacatatcg ctgttcgaga gttcctactt
      421 cggatatcac tgtgttcatg ttgcgccagg caaaccatcc ggcggcggaa aaaatgacga
      481 gcacaacggc aaatcccgct gcgagctttg atccgatctt cacattcttc agcaatcatc
      541 tcacctcaag aaaaaagtgt ggcggaattg tccttttccc cttccaggga gcccccggac
      601 atcccgaatc ggcaaaaact atatcatgaa tacccaggaa caaaaagaaa ggaccgccgg
      661 aattttaacc ggcggtcctt tcttttgctg atgtcacaat tgttagccga ggaaattccc
      721 gagaacctcc agagcttccg ggaagttttc ccttccgagg ccgatgcgga aatggttcca
      781 gtcaacgtcg aacacaaagt cgggaaggac catgagactt ttctcctcca cggcccgctc
      841 gcagaaatcc cggaccggct gggagcccag aagcttggga aatgccgtgg aacccccttc
      901 cggctcgaac cactcaaacc tgtccttccg cccggagaaa aattccctgg caagggcgag
      961 attcctcccc acgatcgcac ggcagcgttc cgcgagttcg tcccgcctcc ggagtgcggc
     1021 gatggccagg atctccgacg gagcggagga acagatggtg gtgtagtcct tcagtgacgc
     1081 cacccggtcg agaagccatt catcctttga agtgatccag ccgatccgca ggcccggcag
     1141 gccataggtc ttggagaggc cgccgagagc cgcgctcttc ctgtagaggg agggaacgga
     1201 aggcaggcgc ttttccggat catgctccag cccgcgatac atttcgtcgc agaagaggaa
     1261 aatgcctttc ttcgacgcga aatccaccac ggcggacagt tctttctccg tcgggagaaa
     1321 cccggtgggg ttgtggggaa agttgatgac gatcagccgg gtattctcct ttgccgcttc
     1381 ctggaggaag ggaaggtcga gtttccatgc cccgttctcc tttttcagat accacctggt
     1441 gacctcgcat ccgagggact ttgggatctc gtacagggac tggtacccgg ggctgaggac
     1501 gatgacatga tccttctcgt ccaggagggc gttcatggtg agaaaaatgc cttcctccgg
     1561 gttgagcaca agggtgtcac cgggggtgac gcctgaggcg gcggcaatct cctccctgag
     1621 caggggatgc cccctggact cggtataggt gagacgaagc tccctccaca gttcggcaat
     1681 tccgggaccg cttccctcca gcagctcttc cgcccggagg gattcgcagt ccgaggggct
     1741 cagcaggtga cgaaccgaaa actcgtatct ggcgaagtac cgctccagaa gaaaatcccg
     1801 caccttcatt caatcacagc cttcgttaaa aattccggac ggaccggtca ttccggctca
     1861 ttatagccgc ctttcccgca aggtgaaaag ggaaaagacg ggaaaatcat cctttccgct
     1921 cttctttcgc ccctttttct cccccgggag attctccggg ggcgaaaaag gccccccgaa
     1981 ggaggcccgg cacctgttac ggatcatttc ttccctttga ccgataccga ggtttcaggg
     2041 acaagccccc ggttctcctc ccctgtccgg aacctggaca cggcatgttc catggcggcg
     2101 ctcaggttga ccagttcctc cgaggcggag gcaatggact ccacgacctt cgactgctcc
     2161 tgcatggccc ccgcgatgcc cgaaacctgg tccgcaattt cggaactgct cttcgccacg
     2221 ctgtccatgc cggcggtcat ttcctcggcg cttgccgact gttcctccat ggtggcggcg
     2281 atggtctgga tgttttccgc cacgttgttc acccgcttca ccacgtcggc gatgaggatg
     2341 ctcgtctcgt ccgcccggac tacgagatcc ttcaggatct tcgacgagtt ggcggaatcc
     2401 cggagggcac tgtccgtacg gctcgagatc tcgccgatga gcttgccgac ctcgcccgcg
     2461 gccctgttcg attcctcggc gagcttgcgc acttcttcag cgaccacggc gaacccccgt
     2521 ccggcttcac cggcccgggc ggcctcgatg gcggcgttca gggccaggag gttcgtctgg
     2581 tcggcgatgg acgtaatgat gctcacaaaa ccggaaatat tcttcacggt cccggcgaga
     2641 ttggtcaccg cgctgctcac atcttcgccg gccttcgccg tctcggtgat caggccggcc
     2701 atgtcctcca cggccttccc gcccttccgg gcggcatcgg agataagggc ggcctcctct
     2761 ccggcctcca ccgccgcctt cgccccagcc tgggctccgg cggccacttc ctccaccccg
     2821 gcattggtct cttccaccga agcggcggcg gattcagtat tcgagctggc cttctccgcc
     2881 atggccatca cttcctcgat ggacgcagtt gattcctcgg cggcggcgct catgtcctca
     2941 gccctggcga gtacggcccg ggagctcttt gagatgtccc gcatcgaccc ggccaccagg
     3001 gtgatcatgg agttgatgcc gtccaccagc agcttgactt cggcaaggct gctccggagt
     3061 tccaccgtgg aggtcaggtc acctgccgcc acgagttcca tcttttcggc
//
LOCUS       DAOTHD010000148         3107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_37101_length_3107_cov_3.476737, whole genome shotgun sequence.
ACCESSION   DAOTHD010000148 DAOTHD010000000
VERSION     DAOTHD010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37101_length_3107_cov_3.476737"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="P5219_03555"
     CDS             <1..549
                     /locus_tag="P5219_03555"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HRX25864.1"
                     /translation="GIEDLVLHPGSSRGNPPEESLDLVAGRLGTVLGRTSHKRVNILL
                     ETMSGQGNNLGADFREFRRIFEALDWDPRLGICLDTCHMFAAGHDFRTEPAYRRLVGQ
                     IDSAVGVDRVRCWHFNDSAHPLGRRLDRHAHIGEGELGYTPFSLILGDPLWEQVPCIL
                     ETPQTGAGYGGDIALLRKLRGG"
     gene            551..1624
                     /locus_tag="P5219_03560"
     CDS             551..1624
                     /locus_tag="P5219_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HRX25865.1"
                     /translation="MTECWPRLVVNAKAIEENARRVVQKCSAAGIYVSGVTKGMCAHP
                     DIVRAMVAGGCSELADSRVGNLAALKAMNTGLPLLLLRIPMLSELPLVVSRADCSLVS
                     TAETVDGLEKACAASGLVHEVIIMVDLGDLREGVWMDDTGRIAEALTRSRRVRCRGVG
                     VNFGCYGGTLPTREKLLQLTDIGKELERELGCHLSVFSGGSTSSLLLLERGEIPPGIN
                     NLRIGEGILLGEDVTSMRSIPWLIRRTMWLEAELVEVRKKPSVPVGPVGADAFGGRPV
                     FEDRGPRTRAIAAVGRQDVRVEGLVPELEGVSILGASSDHLILDVEDSASSLAQGDKL
                     KFFPDYGAMLALATSPYVPFEVV"
     gene            1667..2479
                     /gene="cdaA"
                     /locus_tag="P5219_03565"
     CDS             1667..2479
                     /gene="cdaA"
                     /locus_tag="P5219_03565"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HRX25866.1"
                     /translation="MLDFFRWQDAIDIIVISIIVHRLLLLIVGTRAMQLVKGLLIMGA
                     LAAAARIFDLRTLSWFLGKVLGVLVIAIPIVFQPELRKMLEELGRGGSLWQRKKTGKK
                     AELLSHEVARALSYLKGQKIGALIVLERNTGLKDFWRTAIKLNADITQELLISIFWEG
                     TPLHDGAVIIDRDNIVAASCYLPLTENSDLSRWIGTRHRAALGVTEVSDAIALVVSEE
                     RGEVSLAINGHLSKNLKEAQVQKLLLHYFAGEDEENKSLLDRLQEDIRTLWT"
     gene            2514..>3107
                     /locus_tag="P5219_03570"
     CDS             2514..>3107
                     /locus_tag="P5219_03570"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HRX25867.1"
                     /translation="MAETPKTDDIISSPMFLRVMAVVIAFAFWFYASGSRTTETTRSL
                     SVPLEYLNVPPQTTLKGPVKEVEVVLSGTPGVLSSLSPEAVVCEVDARGLGVGKYRLA
                     VRAIAPKDVKLVDVVPTHVDIELLRYIDRLIPVEVAVDKGLPPGLYLDMVEIVPKDIS
                     VKGSEKDLAKIGTARIAPTLEELKAGGELSLSVEIVRS"
BASE COUNT          644 a          889 c          939 g          635 t
ORIGIN      
        1 ggcatcgagg acctggtgct tcatcccggc tcttccaggg gaaatccccc ggaagagtcc
       61 ctcgacctcg tggccggacg gctgggaaca gtgcttggaa ggacatccca caagagagtg
      121 aacatcctgc tggaaaccat gtcgggccag ggaaataatc tcggagccga tttccgtgag
      181 ttccgccgca tattcgaggc gctggactgg gatccaaggc ttggcatatg tctcgacacc
      241 tgccacatgt tcgccgcggg ccacgatttc cggaccgagc ccgcctaccg ccggctggtc
      301 ggacagatcg attcggcggt gggggttgac agggtccgat gctggcattt caatgacagc
      361 gctcatcctc tcggcagaag acttgaccgc catgcccaca tcggcgaagg tgaactcggg
      421 tatactcctt tttccctgat tctcggggat cccctgtggg agcaggtgcc ctgcattctc
      481 gaaactcccc agaccggtgc cgggtacggt ggagacattg cccttcttcg aaaactgaga
      541 ggaggataac atgaccgaat gctggccccg tctcgtcgta aacgcgaaag caatagagga
      601 aaacgcccgc agggtcgtgc agaaatgctc cgcggcggga atatacgtct ccggcgtcac
      661 caagggaatg tgtgcccatc cggacatcgt ccgggccatg gtggcgggcg ggtgttccga
      721 gcttgccgac agccgggtgg gaaacctggc agccctgaag gccatgaaca ccggcctgcc
      781 ccttcttctt ctccgcatcc ccatgctcag cgaactgccc ctggtggtct cccgggctga
      841 ctgttccctc gtcagcaccg ccgaaacggt ggacggcctg gagaaagcct gcgccgcctc
      901 gggactggtt cacgaagtga tcatcatggt ggacctgggc gatctgaggg aaggcgtatg
      961 gatggacgac accggcagaa tcgctgaagc cctgacgcgt tcacggagag tccgctgcag
     1021 gggagtgggc gtcaatttcg gatgttacgg aggcaccctc ccgacaaggg aaaaactcct
     1081 tcagctgaca gacatcggaa aagaactgga acgggagctc ggctgccatc tttcagtctt
     1141 ttccggggga tccacatctt ccctcctgct ccttgaacgg ggggagatcc caccgggcat
     1201 caacaacctg aggatcggag agggcatcct tctcggcgag gacgttacgt ccatgagatc
     1261 cattccctgg ctcatccgga gaaccatgtg gctcgaggct gaactggttg aggtgagaaa
     1321 gaagccgtcg gttccagtgg ggccggtagg tgccgatgcc ttcggaggcc ggccggtttt
     1381 cgaggaccgg ggaccccgca cgagggccat tgccgctgtg gggaggcagg acgttcgggt
     1441 cgaagggctt gttcctgagc tcgagggtgt ttccatactc ggtgcgtcca gcgatcacct
     1501 gatcctggac gtggaagact ccgctagctc cctcgcccag ggagacaaac tgaagttttt
     1561 ccccgattac ggggccatgc tcgccctggc gacatcaccc tatgttcctt ttgaagtggt
     1621 atagtatagt gcggaatggt tccatattat aacccggagc gaacccatgc ttgatttttt
     1681 ccggtggcag gacgccatcg acataatagt catttccata atcgtccaca ggctgctcct
     1741 gctgatcgtc ggcaccaggg cgatgcagct cgtgaaaggg cttctcatca tgggagccct
     1801 tgccgccgct gcccggatat tcgacctgag gaccctttca tggtttctgg gaaaggttct
     1861 gggagttctg gtcatcgcca ttcctatcgt cttccagccc gagctgcgga aaatgctcga
     1921 ggagctcgga aggggcggga gcctgtggca gaggaaaaaa acaggcaaga aagccgaact
     1981 cctcagccac gaagtcgccc gggccctcag ctatctcaag ggacagaaga tcggcgctct
     2041 catcgtgctt gaacggaaca caggcctgaa ggatttctgg agaacggcca tcaagctgaa
     2101 cgccgacatt acccaggagc ttctgatctc catattctgg gagggaacgc ctcttcacga
     2161 cggagccgtc atcatagaca gggacaacat tgtcgccgcg agctgctacc tgcctcttac
     2221 cgagaattcc gacctctcac ggtggatagg cacccgccac agggccgccc ttggagttac
     2281 cgaggtttcc gacgccatag ccctggttgt gtccgaggag cgaggggaag tctccctggc
     2341 gattaatggc catctgtcca aaaatctcaa ggaggcccag gtgcagaagc tgctgctcca
     2401 ttatttcgcc ggcgaagacg aggaaaacaa atcgctgctc gacagattgc aggaagacat
     2461 ccggaccctc tggacctgag tttttctttt cgctgaggag gctgcggttc aatatggccg
     2521 aaacaccaaa aaccgatgat ataatatcct ctcccatgtt ccttcgtgta atggccgtgg
     2581 tcattgcctt cgctttctgg ttttatgctt ccggaagcag gaccacggag acgacgagaa
     2641 gcctgtccgt tccactggaa tacctcaacg ttcctcccca gacgactctt aaggggccgg
     2701 tgaaggaagt ggaggtggtg ctttccggca ctccgggcgt tctctcgtcc ctttcgccgg
     2761 aagcggtggt ctgcgaggtg gacgccaggg ggctcggcgt gggaaaatac aggcttgccg
     2821 tgagggccat cgcccccaag gatgtgaaac tggtggatgt ggttccgacc catgtcgaca
     2881 tcgagcttct ccggtacatc gacaggctca tccccgtaga ggtggccgtt gacaagggcc
     2941 ttcccccggg gctgtatctg gacatggtgg aaatcgttcc caaggacata agcgtgaaag
     3001 gaagcgagaa agacctcgcg aagataggca ccgctcggat cgctcccact cttgaagagc
     3061 tcaaggcggg gggtgagctt tccctgtccg tggagatcgt caggtcc
//
LOCUS       DAOTHD010000149         3101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_37210_length_3101_cov_2.891005, whole genome shotgun sequence.
ACCESSION   DAOTHD010000149 DAOTHD010000000
VERSION     DAOTHD010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37210_length_3101_cov_2.891005"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="P5219_03575"
     CDS             complement(<1..1024)
                     /locus_tag="P5219_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRX25868.1"
                     /translation="MSDTAVLSLDRIIASSPVVILWGEPEERHPVDFVTGNIARFGYT
                     VSEIVLEEIPFLSLLLPEDRDRVKEEFFGAVRENAPSVLLEYRIVTRTGEARWIEDRT
                     AFHRDGNGKAFAYQSSLLDVTDRKTAELRARESEERLSRVLAASKVGIWEYFPGSDSF
                     SGSGHGEILGLEDGEFPLSREELFGRTLSPKENDGAETAWERFLSGEDSFLDVECRAT
                     LPDGTSRWVVFKGFPVRNGEGSLLRVTGLTIDITSLKEAELRAEAQNRRLELLHSMFL
                     SFMEELDSTALLERILTKACELAGTEHGSLSVYQEKKNTFLRVFGTGLYAPMVNESRP
                     AFVGISG"
     gene            complement(1032..>3101)
                     /locus_tag="P5219_03580"
     CDS             complement(1032..>3101)
                     /locus_tag="P5219_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRX25869.1"
                     /translation="DITDRKNWEQENRRMEENLSAVLAASRIWAWEYLPETDELVGSG
                     CFPVDGSRQSRLCLSRKKLWEDIVSPPDLARIEESWARLLEGETGFSAVEHPVLRPDG
                     TQGWVSVRAFPRLDEKGRVAAVTGLTVDITSLKETLHRTDSQNRRFELLRSLSLGMME
                     DLDTDSLLERILRGAVEFAGSGNGVISLLEQGGTVLRHRCAVGLHEAMVGEGRPAAEG
                     MSGQALRERRRLFAKDYRSWEGRIADPRFDSITANIVLPLFSGPHDFGTVGIHFTGEC
                     PDLDNSFFASLDQFAAAASIALENARLHEASQREIREKIRAQERLTVHAGLAAASAEA
                     SGFLLSQDHGGTALGRALASLAEASGAGRAVLLRNVAQDGLAEVVARGEGGAGLPFSV
                     EDGPVRAEEYPLLFGHLTSGQTYLGPLNRDAGPGIAGVPIFIRSAFWGFLALLFPDGA
                     PCYSAGELDVLRTAAYNLAVSMVRWEADDRVKEGYERLQKTFDDVIRTMGFIVGRKDP
                     YTIEHQERVAVLARGIGAVMGLDPWRLEGLRICSLVHDVGKVEIPGEILSKPGRLSPM
                     EFELIKTHAQSGYDILKEVDFPWPVAEVARQHHERMDGSGYPRGLKGEEILPEARILA
                     VADVVEAMSSHRPYRPSLGLETAVAEIGRKRGTAFDPSVVDACMEVLRKNPGVIAGEV
                     KSFGRGG"
BASE COUNT          576 a         1040 c          929 g          556 t
ORIGIN      
        1 atccggaaat gcccacgaag gcgggacggc tctcgttcac catgggagca tagagccccg
       61 tgccgaagac ccggaggaag gtgttcttct tttcctggta aacggaaagg gatccgtgct
      121 ccgtcccggc gagctcgcag gccttcgtca ggatgcgctc caggagggcg gtggagtcca
      181 gctcctccat aaaggaaaga aacatggaat ggagcagttc aagccgcctg ttctgggctt
      241 cagcccggag ctcggcctcc ttgagactcg tgatgtcgat ggtgagtccc gtcacccgga
      301 gcaagctccc ttccccgttc ctgacgggga accccttgaa gaccacccat cgggaagtgc
      361 cgtcaggcag ggtcgccctg cactccacgt cgaggaagga gtcctcccct gaaaggaagc
      421 gttcccacgc cgtttcggcc ccgtcgtttt ccttcggtga aagggtccgg ccgaagagct
      481 cctccctgga gagggggaac tccccgtcct ccagcccgag gatttcgccg tggccggaac
      541 cggagaagct gtcgctcccg ggaaaatatt cccagatccc cacctttgag gcggcgagga
      601 ccctggagag cctctcctcg ctctccctgg cccgaagctc ggcggttttc cggtcggtga
      661 cgtccagcag gctggactgg taggcgaaag cctttccgtt cccgtcccgg tgaaaggccg
      721 tccgatcctc gatccaccgg gcctcacctg tcctggtaac gatccggtac tccagcagga
      781 ccgaaggggc attctcccgg accgccccga aaaattcctc cttcacccga tccctgtctt
      841 cgggaaggag aagggacaga aagggaattt cctccaggac gatctcggaa acggtatagc
      901 cgaaacgggc gatgttcccg gtgacaaaat ctaccggatg acgctcctcc ggctcgcccc
      961 agagaatcac cacggggctg gaggcaatga tccggtcaag gctgagaacg gctgtgtcgc
     1021 tcatgattct ctcatcctcc tcttccgaag gatttcactt cgccggcgat gacccccgga
     1081 tttttccgga ggacctccat acaggcgtcc acgaccgacg ggtcgaaagc cgttccccgt
     1141 tttctcccga tctccgccac ggcggtttcc aggccgaggg atggccggta gggccggtgg
     1201 gacgacatgg cctccaccac gtccgccacg gcgaggatcc gggcctcagg caggatctcc
     1261 tcccctttca gcccccgggg gtacccggaa ccgtccatgc gctcgtggtg ctgccgggcg
     1321 acctccgcca cgggccaggg gaaatcgacc tccttcagga tgtcgtaccc cgactgggca
     1381 tgggtcttga tcagctcgaa ctccatgggg gacagcctcc ccggcttgct caggatctcc
     1441 ccggggatct ccaccttccc cacgtcgtgg accagggagc agatgcgaag gccttccagc
     1501 cgccaggggt caagccccat aacggcaccg atcccgcggg ccaggacggc cacccgctcc
     1561 tggtgttcta tggtataggg gtcctttctc ccgacgatga agcccatggt gcggatcacg
     1621 tcgtcgaaag tcttctgcag cctttcatag ccctctttca ccctgtcgtc cgcttcccac
     1681 cggaccatgg agaccgccag gttgtaggcc gccgtcctga ggacatccag ctctccggcc
     1741 gaataacagg gggccccgtc cggaaagagc agggcgagga atccccagaa cgcggaccgg
     1801 atgaaaatgg gaaccccggc gattcccggc cccgcatccc ggttcagggg accgaggtag
     1861 gtctggccgg aggtgaggtg cccgaagaga aggggatatt cttccgcccg gaccggacca
     1921 tcctccacgg agaacggaag gcccgctcct ccttctcccc ttgcaaccac ttcggcaagg
     1981 ccgtcttgcg ccacgtttct gagaaggacg gcccgcccgg cccccgaggc ttccgccagg
     2041 gaagccagcg cccggccgag ggccgttccg ccgtggtcct gggagagaag gaatccggac
     2101 gcttcagccg aggcagcggc aagcccggca tggacagtaa gcctctcctg ggcacggatt
     2161 ttttccctga tctcccgctg ggatgcctcg tggagcctgg cgttttccag ggcaatggag
     2221 gcagcggcgg cgaactggtc gagggaggcg aaaaaggagt tgtcgaggtc cgggcattca
     2281 cccgtgaaat ggatgcccac cgtgccgaag tcgtgggggc cggaaaagag cggcaggaca
     2341 atgttggcgg taatggagtc gaacctggga tcggcaatcc gcccctccca tgagcggtag
     2401 tccttcgcaa aaagccgacg tctctcccgg agcgcctgcc cgctcatgcc ctccgccgca
     2461 gggcgccctt cccccaccat ggcctcgtga agtccgacgg cgcacctgtg gcggagcacg
     2521 gtcccaccct gctccagcag ggagatcacc ccgttccccg atccggcgaa ctccacggcg
     2581 ccccggagaa tcctctccag cagtgaatcc gtatcaaggt cctccatcat cccgagggac
     2641 agggaacgca gcagttcaaa acgcctgttc tgggaatcag tcctgtgaag ggtttctttc
     2701 agggaggtga tgtccacggt caggcccgtc accgccgcaa cccggccctt ttcatccagc
     2761 cggggaaaag ccctgacgga tacccagccc tgagttccat ccggccgcag aaccgggtgt
     2821 tccactgcgg aaaaacctgt ctctccttcc agaagacggg cccaggattc ctctatcctg
     2881 gccagatccg gcggagagac aatatcctcc cagagtttct tacgggaaag gcagagcctt
     2941 gactgtctgc tgccgtccac ggggaagcag cctgaaccca caagttcgtc cgtctccggg
     3001 aggtactccc acgcccagat cctggaggcg gccagcacgg cagacaggtt ctcctccatc
     3061 cggcggttct cctgttccca gtttttcctg tccgtgatgt c
//
LOCUS       DAOTHD010000150         3073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_37645_length_3073_cov_3.834327, whole genome shotgun sequence.
ACCESSION   DAOTHD010000150 DAOTHD010000000
VERSION     DAOTHD010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37645_length_3073_cov_3.834327"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1273
                     /gene="hflX"
                     /locus_tag="P5219_03585"
     CDS             149..1273
                     /gene="hflX"
                     /locus_tag="P5219_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRX25870.1"
                     /translation="MPERKAVIAGLELPGQPFSPSVLLEEVELLLRNLGITSAGTVIQ
                     RRSRPDPAFLLGKGKAEELALFCKTLGATLLVCNESLTPGQKNNLQRTTGVEVWDRPF
                     VIMKIFELRASSSEAKMQVEMALCKYEIPHLKGLGAQMSRLGGGIGTRGPGETEFERH
                     RRKLERRVRDIGKKLGILKRKRTLQRDRRKKMNLPVVSLVGYTNSGKSSLLRALSGDA
                     SLVAENRLFSTLDTFMRKVRLPSGREILLSDTVGFIRDLPPGLVAAFRTTLEEIVSSS
                     FLVFVLDAAAADFQEIREVVEKTVQEIGGGDIPRFFALNKCDLLAADTKRVMEEKFRE
                     AGEAAVFISALSGTGLSELLATLDAFLLKTETISREGDGD"
     gene            1273..2157
                     /locus_tag="P5219_03590"
     CDS             1273..2157
                     /locus_tag="P5219_03590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HRX25871.1"
                     /translation="MIRSMTGFTRESRNFEWGTLTVEISSVNHRYQELSIRLPRELAS
                     FESTIGGLLRSGLGRGKIRFFAEITWAPRYRALAIDGDVLSNYYTQILSLSEKLGTGQ
                     KPGLQSLLSLPGVLDSPAVLSMVEGQAGGVLEEIVGTGIQNLAGMREKEGLNLGGAIE
                     GYLGAFESLVDSIEEYWTGKKAGLFEELRKRVTLLLEGVTTEADQGRVAQELALMGDK
                     WDISEEFVRSRSHCRQFRTILKGPSSEGRKLDFLIQEMNREVNTMGSKITDAELRWMV
                     VEAKTLLEKIREQVQNVE"
     gene            2163..2432
                     /locus_tag="P5219_03595"
     CDS             2163..2432
                     /locus_tag="P5219_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HRX25872.1"
                     /translation="MTAKLVHIGFGNMIVAERIVAVIHPTSAPIKRLKEEAKEDGRLI
                     DATQGRKTRAILITDSNHVVLSAIQPETIVNRFEEDIEDESVVQG"
     gene            2410..2997
                     /gene="gmk"
                     /locus_tag="P5219_03600"
     CDS             2410..2997
                     /gene="gmk"
                     /locus_tag="P5219_03600"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRX25873.1"
                     /translation="MNRSYKGKLFVLSGPSGAGKGTLRKKVFETVEDIRFSISCTTRP
                     PRQGEKDGVDYRFISEEAFLTLLEEDRFLEYAKVHGHYYGTLRDDVERTLSSGIDMVL
                     EIDVQGAFQVREKMPESILVFVSPPSLEELERRLRERGTESGENLRIRLRNARLEMLK
                     SGDYDYVIVNDDAERASNELKSIITGFRPFREERK"
BASE COUNT          773 a          758 c          896 g          646 t
ORIGIN      
        1 ccctccatct ttcggtggtt gtcggcggga caattgatac aactgattat gcaggccgtg
       61 gcagggaggg gagagaactg ctgctcagaa aacttgagga agagctggtt tcccttgagc
      121 cgaaggcccg ggccctcctt tgacggccat gccggaacgt aaggcggtga tcgcgggact
      181 ggaactcccc ggtcagccct tttctccttc ggttcttctg gaggaggttg aacttctcct
      241 caggaacctg ggtataacgt ccgcaggtac ggtcatccag cgtcgaagca ggcccgaccc
      301 ggcatttctt ctcggaaaag gcaaggcgga agaactggct ctcttctgca aaacccttgg
      361 cgccacactt cttgtttgca atgaatccct gaccccgggg cagaagaaca acctccagag
      421 aacaactgga gtggaggttt gggaccgacc ctttgtcata atgaaaatct tcgagcttcg
      481 ggcgagttct tccgaagcaa agatgcaggt cgaaatggct ctgtgcaaat acgaaattcc
      541 tcacctgaag gggctgggag cccagatgtc ccgtctcggg ggcgggatcg gaacaagagg
      601 tcccggagaa accgaattcg aacggcacag gagaaaactt gagcgtcgcg tcagggacat
      661 cggcaaaaaa ctcgggatac tcaaaagaaa gaggaccctc cagcgcgacc ggaggaagaa
      721 aatgaacctt cccgttgtct cgctggttgg atatacgaac agcgggaaat cctcccttct
      781 ccgggccctg agcggcgatg cgtcccttgt ggcggagaac aggctttttt ccaccctgga
      841 tacgttcatg aggaaagtga gacttccatc ggggagggag atcctgctgt cggatacggt
      901 gggcttcatc agggaccttc ctcccggtct cgtggcggct ttcaggacaa cacttgaaga
      961 aattgtgtcg tcctcgttcc ttgtttttgt tctcgacgcc gctgcggctg atttccagga
     1021 aatacgggaa gtcgtcgaga aaacggttca ggagatcggc ggaggggata tcccccgttt
     1081 ttttgccctg aacaaatgcg atcttcttgc cgcggataca aaacgggtga tggaggagaa
     1141 gttccgggag gcaggcgaag cagccgtttt catcagcgcc ctttccggaa ccgggttgtc
     1201 cgagctgctt gcgacccttg acgcctttct cctaaaaacg gagacgattt ccagagaagg
     1261 ggatggggac tgatgatacg cagcatgact ggttttacaa gggaaagcag aaactttgag
     1321 tggggaactc tcacagtgga gatttcatcg gtgaaccaca ggtaccagga actttcgatc
     1381 cgcctgccga gagagcttgc atcctttgaa tcaacaatcg gcggccttct ccgttcaggc
     1441 ctgggcaggg gaaaaatacg gttcttcgct gaaatcacct gggctccgag gtacagggca
     1501 ctcgccattg acggggacgt tctcagcaat tattataccc agattctctc tctctccgaa
     1561 aaactcggca cagggcagaa accgggactc caatcccttc tttcccttcc gggggttctc
     1621 gactcgcccg ccgttctctc catggtggag gggcaagcgg gcggggtcct tgaagagatc
     1681 gtgggcaccg gaatacagaa cctggccgga atgcgggaaa aagaaggcct taacctgggg
     1741 ggggcaatcg agggatatct cggcgccttt gagtcccttg tcgattccat agaagaatac
     1801 tggactggaa agaaagcagg actttttgaa gaactccgga aacgggtaac acttctcctt
     1861 gaaggagtaa cgacagaagc tgaccaggga cgggttgccc aggaactcgc cctcatggga
     1921 gacaaatggg acatatccga ggagttcgtc cgttccagaa gtcattgcag acagttccga
     1981 accatactta aaggaccttc ttccgaggga agaaaactcg atttcctgat ccaggaaatg
     2041 aaccgggagg tcaataccat gggctcgaaa ataacggacg ctgagcttcg ctggatggtg
     2101 gtggaggcaa agactctcct ggaaaaaatc agggaacagg ttcagaacgt ggagtgagac
     2161 tgatgaccgc aaaactggtt catatcggtt tcggaaatat gatcgtggcg gaaaggattg
     2221 tggccgtcat tcatccgacc tctgctccca tcaagcgcct caaggaagag gccaaggaag
     2281 acgggaggct catcgacgcc acacaggggc gcaaaacgag ggctattctg ataaccgaca
     2341 gcaaccacgt ggtgctgtcg gccattcagc ccgaaactat cgtgaacaga tttgaggaag
     2401 atatcgagga tgaatcggtc gtacaagggt aaactcttcg ttctttccgg cccgagcggc
     2461 gccggaaagg gaacgctgcg gaaaaaagta ttcgaaacag tggaggacat ccggttttcg
     2521 atctcatgca ccacgaggcc tccccgccag ggagagaaag acggtgttga ctaccgcttt
     2581 atatccgaag aagcctttct gacccttctg gaggaggaca ggtttctgga gtacgcgaaa
     2641 gtgcatggcc attactatgg gaccctcagg gacgacgtgg agcggaccct ttcgagcggc
     2701 attgatatgg tactggaaat agatgtgcag ggggctttcc aggtcaggga aaaaatgcct
     2761 gagagcatcc ttgtgttcgt ctctcctccc tctctcgaag aacttgagcg gcggctcagg
     2821 gaaagaggaa cggaaagcgg tgaaaatctc aggatcaggc tccggaatgc aaggcttgag
     2881 atgttgaaat caggggatta tgattacgtt attgtgaacg acgacgccga acgggcttca
     2941 aatgaactga agtccataat aaccggattc agaccattca gggaggaacg gaaatgattt
     3001 tctacgatat ggattctctg gcggaaaaac agggcataaa caacaaatat cttctcactg
     3061 ccgcagtggc tgc
//
LOCUS       DAOTHD010000151         3068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_37738_length_3068_cov_3.347162, whole genome shotgun sequence.
ACCESSION   DAOTHD010000151 DAOTHD010000000
VERSION     DAOTHD010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37738_length_3068_cov_3.347162"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="P5219_03605"
     CDS             complement(<1..182)
                     /locus_tag="P5219_03605"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRX25874.1"
                     /translation="MKFEEKFVSYEGFTFDDVLLEPSYSEVLPSDVCIRTRLTESVSL
                     NIPLCSSAMDTVTESRL"
     gene            complement(353..>696)
                     /locus_tag="P5219_03610"
     CDS             complement(353..>696)
                     /locus_tag="P5219_03610"
                     /inference="COORDINATES: protein motif:HMM:NF014588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRX25875.1"
                     /translation="IGTLYPASDMKYKGISSLVLLEDVARRASSAGWCLEWADCVVIA
                     QEPRLSSRLQDMVRAMEQALPPEWRNRIHLKAKSGEGEGAVGSCCSVVCHAAATLSAC
                     GLRREELDERR"
     assembly_gap    697..706
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<707..1515)
                     /locus_tag="P5219_03615"
     CDS             complement(<707..1515)
                     /locus_tag="P5219_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HRX25876.1"
                     /translation="MEGFSLVVVAAGRGERAGGDSPKQYRRLGGRMVWEWSALLAESL
                     FAEGIVRQVVFVVPPGDEPLFGEKLSAFSCPFSVTAGGAERNDSVLNGLRCATGDFVL
                     VHDGARPFAGKILCRRIMEAVTEDRGIVPLLPVSDALKKEETAGCFQPFPREGLFLTQ
                     TPQGFPKDKLIRALCSHGEGAKDEGEAWIRAGHDLGVVAGDRSNTKITWPEDFVLGEN
                     RFQKTFRTGIGYDIHSLVPGRRFILGGVAFPGFPPEAKGEAGKNNPSESESP"
     gene            complement(1515..2702)
                     /locus_tag="P5219_03620"
     CDS             complement(1515..2702)
                     /locus_tag="P5219_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HRX25877.1"
                     /translation="MTEGFGKIVRMIFSGLVLLLGGMIGAQVAQLLFPLLPEDWFIAG
                     KTWLPMWLPGTIFLTVLSVVLFASLGLMLSPVFLRMLGLMGTFFETHLKNTSWSEITA
                     ATIGMIVGLIIANLVALPFSDLLFGSYFALILNIVLGYLGARIMLKRQSDLPHVLSPI
                     QGLKEKISMIRGQRDRHDDEEMHLSPEERQWCAGRKILDTSVIIDGRILDVARTGFLE
                     GVVIIPQFVLLELQAVADSTDPARRTRGRRGLDVVNELQRLGELQVEIADTPLKSLKA
                     ESVDSGLVALAGRLGGQILTTDYNLNKLAQIQGIRVLNVNDLANALKPMLLPGETIII
                     DVIREGKEPHQGVGYLDDGTMFVVEDGENFIGKRVEVVVTSMLQTSAGRMVFGRVRKE
                     VRS"
     gene            complement(2797..>3068)
                     /locus_tag="P5219_03625"
     CDS             complement(2797..>3068)
                     /locus_tag="P5219_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRX25878.1"
                     /translation="LDGEDLDEECLARLVRRIGEEGVREVIIATNPRIEGDLTYHAIV
                     DRLGETDVSISRLAYGLPVGGSIEYADRTTLLASLESRVTVKRKE"
BASE COUNT          662 a          941 c          819 g          636 t
ORIGIN      
        1 agacgggatt cggtcaccgt atccatggct gaactgcaga gaggaatgtt gagggagacg
       61 ctctccgtca gcctggtcct gatgcacacg tcggaaggga gtacttcgct gtagcttggt
      121 tcaagaagga catcatcaaa ggtgaagcct tcataggaaa cgaatttttc ttcaaatttc
      181 atcgggaaac ctccttgccc ttcggggggg cgaaaagact ttgggaaatt gtaggccgaa
      241 gccggaaaag aaacaacccc ccgaaaaaaa acggagatga gaaatgaagt acgggccttc
      301 ggcggtcata tactccatcg aaggcccgtg cattctcacc gtaattctgg cgtcagcgcc
      361 tttcatccag ctcttcccgc ctcagcccgc aggcggaaag agtggcagcg gcatgacaga
      421 ccacggaaca gcagcttccg acagccccct cgccttcacc ggatttcgcc ttcaggtgga
      481 tcctgttcct ccattccgga ggaagggcct gctccatggc ccgcaccatg tcctgcaggc
      541 gggaggaaag acggggctcc tgggctatca ccacacagtc cgcccattcg aggcaccagc
      601 ctgccgaaga ggctctccgg gcgacatcct ccaggagcac aaggctcgaa atccctttgt
      661 atttcatatc cgatgcggga tacagggttc ctatatnnnn nnnnnnggcg attcactctc
      721 ggaggggttg tttttcccgg cttccccctt ggcttcaggg ggaaagccgg gaaaagcaac
      781 ccctccgaga atgaatcgcc ttcccggaac gagggaatgg atgtcatatc ctatccctgt
      841 tctgaaggtt ttttgaaacc ggttttcacc aaggacaaaa tcctccggcc atgtgatctt
      901 cgtgttgctc cggtctcccg ccaccacgcc gagatcatgg cccgcccgta tccaggcctc
      961 tccctcgtct ttcgctcctt ccccgtggga acaaagagcc cgtatcagct tgtccttcgg
     1021 aaatccctgg ggtgtctggg tgagaaaaag cccttcccgc gggaagggct gaaaacagcc
     1081 cgctgtctct tccttcttga gggcgtcgga cacaggaaga agtggtacga ttcccctgtc
     1141 ctccgtcacc gcttccatga tccgccggca cagtattttt cccgcgaagg gtctggctcc
     1201 atcgtggacg agaacaaagt cacctgtcgc gcaccggaga ccgttaagaa cagaatcatt
     1261 tctttcagca ccccccgcgg tcacggagaa tgggcacgaa aaggcagaga gcttttcccc
     1321 aaaaaggggt tcatctcccg gggggacgac aaagacaacc tgcctcacga tgccttcggc
     1381 aaagaggctt tcggccaaca gggcgctcca ttcccacacc atgcgccctc caagcctgcg
     1441 gtactgcttt ggggaatccc ccccggcccg ttctccccgg cctgcagcca ccaccaccag
     1501 ggaaaaccct tccatcagga ccggacttcc ttcctgaccc ggccgaagac cattctgcct
     1561 gcggaggtct ggagcatgga ggtgacaacc acctcaaccc gcttgccgat gaaattctct
     1621 ccgtcctcga cgacgaacat tgtcccgtca tccaggtagc ccacaccctg gtgaggctcc
     1681 ttcccttcac ggatgacgtc aatgatgatg gtttctcccg gaaggagcat gggtttcaga
     1741 gcgttggcga ggtcgttcac gttgagcacc ctgattccct gaatctgggc gagcttgttc
     1801 agattgtagt ccgtggtgag aatctggccc ccgagcctgc ccgccagggc aacaagcccg
     1861 ctgtccaccg attcggcctt caggcttttc aggggtgtgt cggcaatttc cacctggagt
     1921 tcccccaggc gctggagctc gttcacaaca tccagccctc tccttcccct ggtccgcctg
     1981 gcggggtcgg tggaatccgc gacagcctga agctccagga gaacgaactg ggggatgatg
     2041 accactccct cgagaaaccc cgtccgtgcc acgtccagga tccttccgtc gatgatgacg
     2101 cttgtgtcga ggatctttcg cccggcgcac cactggcgct cttcgggaga caggtgcatt
     2161 tcttcatcgt cgtgacggtc ccgctgcccc ctgatcatgg agattttttc cttcagcccc
     2221 tgaatggggc tcaggacatg ggggagatca ctctgccgtt tcagcataat cctcgcgccg
     2281 agatacccga ggacaatatt caggatcagc gcgaaatagc ttccgaagag aaggtccgaa
     2341 aagggaaggg ccacgagatt cgctatgatg aggccgacga tcatgccgat ggtagcggct
     2401 gtgatttccg accagctggt gttcttcagg tgtgtttcaa aaaaagtgcc cataagaccg
     2461 agcatccgca aaaagacggg ggaaagcatc aagccgagtg aagcgaataa aaccaccgat
     2521 agtacggtaa gaaaaattgt gccgggcagc cacatcggca gccatgtttt tcccgcgatg
     2581 aaccagtctt ccggaagaag gggaaaaaga agctgggcca cctgggctcc gatcatccct
     2641 cccaaaagaa gaacaagccc ggaaaagatc atcctgacga tcttcccaaa accctccgtc
     2701 atagtttcac ctctcttgca gccggtcaat ccccttcccc gccgggaagg ggcaaatgat
     2761 tccagtggcg ctccgttccc tcccggaggg aacggttcat tcttttcttt ttacggtcac
     2821 ccgggattcg agggaggcaa gaagggtcgt cctgtcggca tactctatgc ttcctcccac
     2881 gggcagacca taggccagcc gcgagatgga gacgtcggtc tctcccagcc tgtccactat
     2941 ggcatggtac gtcaggtctc cttcgatcct cgggttcgtg gcgataatca cctccctcac
     3001 accctcttca ccgatacggc gcacgagcct ggcgaggcat tcctcgtcca gatcctcgcc
     3061 gtcaaggg
//
LOCUS       DAOTHD010000152         3063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_37817_length_3063_cov_3.234375, whole genome shotgun sequence.
ACCESSION   DAOTHD010000152 DAOTHD010000000
VERSION     DAOTHD010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37817_length_3063_cov_3.234375"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="P5219_03630"
     CDS             <1..541
                     /locus_tag="P5219_03630"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRX25879.1"
                     /translation="RGLRQAAELGSALRPYSPEAVMTSPLLRAKSTARAIAGACSIRV
                     EEDEGFNNISLGIWEGQPKTEIAERYPGQWKIWLENPERLAVEGAESLDDVLRRSMTA
                     LDRGVAKYRGKTFAAVTHRTVIKPLLAGCLGIASPYFWKVHMDTGSYSILVHDDLHGY
                     SLFSLNRTDHLSGFITEWV"
     gene            622..1836
                     /gene="folP"
                     /locus_tag="P5219_03635"
     CDS             622..1836
                     /gene="folP"
                     /locus_tag="P5219_03635"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRX25880.1"
                     /translation="MIVYPLPAQSREDLFSALRRIGADLRSNAYFEPKRNLLSLYIPG
                     VDFRAAAFLKQELLARGGDAVVNCGVISCEASESAVLLLGTPGQLRSLAEKLGALPCW
                     GLPEIREGLSRTLAGLDKSAWTFSFREGRALPLGKRTAVMGILNLTSDSFYEKSRISP
                     EDDILSRARSMVEAGAAVLDLGAESTRPGSEPVPEQEEMERLIPAVAAVRKAFPETVL
                     SADTWKAKVALEAVRAGADIINDISGLGFDPDLPGAVAESGAALVLSHIQGTPRDMQK
                     DPRYDDTVADILRSFEERLSLAEKAGIPAERIILDPGLGFGKRCGDNLRILRNLDAFR
                     IFGRPLLIGHSRKGFIGATLGLADPEDRLEGTLAVTALCGLRNVALVRVHDVEENVRV
                     LSMLSAIGECME"
     gene            1833..2327
                     /gene="folK"
                     /locus_tag="P5219_03640"
     CDS             1833..2327
                     /gene="folK"
                     /locus_tag="P5219_03640"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRX25881.1"
                     /translation="MSTVALSLGSNLGDRLQLLRKAVSLLRGAGLVILASSDVFETPP
                     WGVENQPRFLNACVLAETRLSPEELLDLLKQMEDALGRTPEKRWGPRIIDIDILFCDG
                     VSVRSDRLTIPHREMARRAFVLLPLAQVAPEWRHPETGLTPEEMFRLLPTAEAGGILR
                     ICTL"
     gene            complement(2386..2850)
                     /locus_tag="P5219_03645"
     CDS             complement(2386..2850)
                     /locus_tag="P5219_03645"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(2722..2724),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRX25882.1"
                     /translation="MREGDGLPPFRLSDGGGKEWTAADFEGRWCVLFFYSKDNTSGUT
                     SEVRDFADRMPAFEALNCAVAGISPDSQESHRKFAEKLGISFPLLSDPDHGFIEACGF
                     WAAKKMYGREYMGVERSTVIADPGGKAVKIFRKVKVSGHAEEVLQTLKEHAG"
     gene            complement(2868..>3063)
                     /locus_tag="P5219_03650"
     CDS             complement(2868..>3063)
                     /locus_tag="P5219_03650"
                     /inference="COORDINATES: protein motif:HMM:NF023785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25883.1"
                     /translation="EDFGKAMELALLQEGPFPLGVIYRKEGVPVFEETLAAYRKDDRP
                     LFRREAPRKAVKELLASLAG"
BASE COUNT          551 a         1026 c          896 g          590 t
ORIGIN      
        1 gcggggcctc cggcaggccg ccgaacttgg gtctgccctt cgcccctata gccctgaagc
       61 ggtgatgaca agccccctgc ttcgggcaaa aagcaccgcc cgggccattg ccggcgcctg
      121 ttccatccgg gtggaagagg acgaaggatt caataatatc tccctcggca tctgggaagg
      181 gcagccgaaa accgaaatag cggagcggta ccccgggcag tggaaaatct ggctggaaaa
      241 ccccgagcgc ctcgccgtgg aaggggcgga atctctcgac gatgttctga ggcgctccat
      301 gaccgccctc gaccgggggg tggcgaaata ccgggggaag accttcgccg cagtgaccca
      361 caggacggtc atcaagcccc ttctcgcggg gtgcctcggt atagcctcgc cctatttctg
      421 gaaggtccac atggacacgg ggtcctactc cattctcgtc cacgacgacc tccacggcta
      481 ttccctcttc tcgctgaaca ggacggacca cctttcaggc ttcatcaccg aatgggtgtg
      541 aaagccatcc cttgaccgcc cggaaaggac actatatcat cgggacagcg tttcctacat
      601 tcccgggaag gagctcccgc cgtgatcgtt tacccgcttc ctgctcaaag ccgtgaagac
      661 ctcttctcgg cactgcgccg catcggcgcc gatcttcgct ccaacgcgta ctttgaacca
      721 aaaaggaatc ttctctccct ctacattccg ggagtcgact ttcgggccgc ggcgttcctg
      781 aagcaggagc tgctcgcccg gggcggggac gccgtagtga actgcggagt catctcctgt
      841 gaggcgtcgg agagcgccgt gctcctcctg gggaccccgg gccagctccg gtccctggcg
      901 gagaagctgg gggccctgcc ctgctggggc cttcccgaaa tccgggaggg tctgtcccgg
      961 acccttgccg gcctggacaa gtccgcatgg acattttcct tccgggaggg cagagccctt
     1021 cctctcggaa aacgaacggc cgtcatgggt atcctgaacc tcacatccga ctccttttac
     1081 gaaaagagcc ggatttctcc tgaagacgac atcctgtccc gggcgaggtc catggttgaa
     1141 gcaggcgccg ccgtcctcga cctcggtgct gaatccaccc ggcccggctc ggaaccggtg
     1201 cctgaacagg aagaaatgga acggctgatc cccgctgtcg cggccgtccg gaaggccttt
     1261 ccggaaacgg tgctctccgc cgacacatgg aaggcgaagg tagccctgga agccgtccgg
     1321 gcaggtgccg acatcatcaa cgacatctcc ggcctcggct tcgacccgga cctccccgga
     1381 gcggtggcgg aatcaggagc ggcccttgtc ctctcccaca tccaggggac accccgggac
     1441 atgcagaagg atccccggta tgatgacacc gtggcagaca tcctccgctc tttcgaggaa
     1501 cggctctccc tggccgaaaa ggccgggatt ccagcggaac ggattatcct cgacccggga
     1561 ctgggcttcg gcaagcgctg cggggacaac ctcaggatcc tgcggaacct ggacgcattc
     1621 cgcattttcg gacggcccct cctcatcggc cattctcgga agggattcat cggcgccacc
     1681 ctcggtcttg ccgacccgga ggaccggctg gagggaaccc tggctgtcac ggccctctgc
     1741 ggcctgcgca atgtcgccct cgtccgggtt cacgacgtgg aggaaaatgt ccgggttctc
     1801 tccatgctca gcgccatcgg ggagtgcatg gaatgagcac cgtggccctt tccctgggca
     1861 gcaacctcgg agaccggctg cagcttctcc ggaaagccgt ctccctcctc aggggcgccg
     1921 gcctcgtgat cctggcctca agcgacgtgt ttgaaacccc tccctggggg gtggagaacc
     1981 agccgcggtt tctcaacgcc tgcgtcctgg cggaaacgcg tctttccccg gaagagcttc
     2041 tcgaccttct gaagcagatg gaggacgccc tgggcaggac ccctgaaaaa cggtgggggc
     2101 ccaggatcat cgatattgat attcttttct gcgacggggt gtcggtgcgc tcggaccgcc
     2161 tgaccatccc tcacagggag atggcgcgga gagccttcgt actgctgccc ctggcccagg
     2221 tggcccctga atggcggcac cccgaaacgg gactcacgcc ggaggaaatg ttccgccttc
     2281 tgcccacggc cgaagccggc ggcatcctcc ggatctgcac cctgtgatac ggcatggcat
     2341 gacaagaggc ggagagcatt ctccgcctct tgattttccc gaacgtcagc cggcgtgttc
     2401 cttcagtgtt tggagaactt cctcagcgtg gccggacacc ttcactttcc tgaagatctt
     2461 cacggccttc cccccgggat cagcgatgac cgtggagcgc tccaccccca tatattccct
     2521 gccgtacatc tttttcgccg cccagaaccc gcaggcttcg atgaatccgt ggtccgggtc
     2581 gctcagcagg ggaaaggaaa ttcccagttt ctccgcgaac ttcctgtggc tctcctgaga
     2641 gtcggggctg attcctgcca ctgcgcagtt cagcgcttcg aatgcgggca tcctgtccgc
     2701 aaagtccctc acttcgctgg ttcaccccga ggtattgtcc ttgctgtaga aaaagaggac
     2761 acaccatctt ccttcgaaat cagccgccgt ccattctttt ccaccgccgt cggaaaggcg
     2821 gaacggaggc agcccgtctc cttctctcat gtttttccct ccctccccta cccggccaga
     2881 gaggccagga gctcctttac tgccttccgg ggggcctccc ggcggaacag gggccggtcg
     2941 tccttccggt aagccgccag ggtttcctcg aagacgggaa ccccttcctt cctgtagata
     3001 acccccagag ggaagggccc ttcctggagg agggccagct ccatggcctt gccgaaatct
     3061 tcc
//
LOCUS       DAOTHD010000153         3051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_37997_length_3051_cov_2.806075, whole genome shotgun sequence.
ACCESSION   DAOTHD010000153 DAOTHD010000000
VERSION     DAOTHD010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37997_length_3051_cov_2.806075"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="P5219_03655"
     CDS             <1..715
                     /locus_tag="P5219_03655"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HRX25884.1"
                     /translation="QMRKISPEMTSSQFLDLDSDWNDVLAPGTGGKSGQSSGKGRLRG
                     HFDGASRGNPGEAGAGAVLFDGRGTPVWECARPLGKKTNNEAEYLALLLLLEELERRG
                     VPADIRGDSRLVVNQVTGRWKINEPRLRELADRAMELLRSTKSSLSWVPREENSAADR
                     LSNIALDGPKEGERGASPRERPAFDPDRLERIHDTILIAHGTEDYAVDLLHRACTCPA
                     FQRSRQCKHLDAALVRFGK"
     gene            802..1278
                     /locus_tag="P5219_03660"
     CDS             802..1278
                     /locus_tag="P5219_03660"
                     /inference="COORDINATES: protein motif:HMM:NF025701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HRX25885.1"
                     /translation="MIAGLALFLFAALLAPGAAGTAEACSACGDDDGSVVTFVPVGRG
                     SYGGVSDRRFIVVKDENEWKELWGEINGNVLPLPPLPDIDFSRHALAAVFQGLKRSGG
                     YSISVEAIIETGDRVTVSVREREPGPQNLVTMALSSPWEVVAFPLPQKPVLFTSVQ"
     gene            complement(1368..1640)
                     /locus_tag="P5219_03665"
     CDS             complement(1368..1640)
                     /locus_tag="P5219_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25886.1"
                     /translation="MATPFVTGAAALVSSRFPGESMDQIRSRILGGVDRLSSLSGKVS
                     TGGRLNLARSLGTAVPDDGSGGGGGCSVAGIPSLFFLLPLLLLVRK"
     gene            complement(1622..>3051)
                     /locus_tag="P5219_03670"
     CDS             complement(1622..>3051)
                     /locus_tag="P5219_03670"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HRX25887.1"
                     /translation="FEALSSEQEKNVVFLRVPGKSTEELLETFSSDPAVESVSPNYIL
                     RILSPVIPNDPLFSSQWGLHNTGQDGGRAGADIDAPAAWGVRTSARPEKVVAIIDTGI
                     ASLHEDLRDNLWAGPGGIHGRNCVDDTDDPEDDNGHGTHVAGIIGAKGNNGIGTAGVA
                     WNVKLMAVKMLDYQGSGTSENELEAYDWILRKKQEGVDIVAVNASYGGGGASDAARDA
                     IEALGNAGILFIAAAGNSAEDNDGTPHYPSSYPLPNIISVSATDRNDALSSFSNYGRT
                     SVHLAAPGTAILNTWVYEPSASDIFFDDMESGAGKWTTHVENPSPEIHWRIIDSTRDN
                     RPTKVWTDGPDQHYPINQRTYLSVQNDIDLSGTAGQPVLFGMKIRCDLESGFDYLYLQ
                     FSKDGGATWRNAHEFTGGTTGRTLRSRFPRNSGRRTSVSGFSSKRTTPTFCSTTQGYR
                     STTWEWAGPRIPISLSREPPWRPPS"
BASE COUNT          548 a          907 c          980 g          616 t
ORIGIN      
        1 tcaaatgaga aaaatatcac ctgaaatgac atccagtcaa ttcctggacc ttgattccga
       61 ctggaacgac gttctcgctc ccggcaccgg aggcaagagc ggccagtctt cgggcaaggg
      121 caggcttcgg ggacatttcg acggagcctc caggggaaat cccggggagg ccggagcggg
      181 ggcggtgctc tttgacggcc gggggacccc cgtctgggag tgcgcccggc ccctgggaaa
      241 gaagaccaac aacgaagccg agtatctcgc tcttctcctc ctgcttgagg aactggagcg
      301 ccggggcgtt cccgccgaca tccgggggga cagccggctc gtggtgaacc aggtgacggg
      361 ccggtggaag atcaacgagc cccggctgag agagcttgcc gaccgggcga tggagcttct
      421 caggagcacg aaaagcagcc tctcctgggt gcccagggaa gagaactcgg ctgcggaccg
      481 gctttccaac atcgccctcg acggaccgaa ggaaggggag cgcggcgctt cgccgaggga
      541 acggcccgct ttcgatcccg acaggctgga gcggattcac gacaccatcc tgattgccca
      601 cggaacggag gactacgcgg tggacctcct tcacagggcc tgtacctgtc ccgccttcca
      661 gcggtcgaga cagtgcaagc acctggatgc ggcacttgta cggttcggaa aataagccct
      721 tttacgcatc agccttccca atctttctgc tattcttttt aatgaaaagc agcctggaaa
      781 tggagggatg ttccggtgaa gatgattgcg ggtctggccc tgtttctttt tgcggccctg
      841 ctggcccccg gagccgccgg gacggctgag gcctgttccg cctgcgggga tgacgacgga
      901 tcggtagtga ccttcgttcc ggtgggacgg gggagttacg gcggggtttc cgaccggcgg
      961 tttatcgtgg tgaaggacga gaacgagtgg aaggagctgt ggggcgagat caacggcaac
     1021 gtgcttcccc ttccgcctct gccggatatc gatttctcac ggcatgccct tgccgccgtc
     1081 ttccagggtc tcaagcggag cgggggctat tccatttccg tggaggccat catcgaaacc
     1141 ggcgaccggg tgaccgtctc ggtccgggag agggagccgg ggccccagaa ccttgtcacc
     1201 atggcccttt cgagcccctg ggaggttgtg gcgtttcccc tgccgcaaaa acccgtactc
     1261 ttcacctccg tccagtagga agcggggaac aggacaaata agaagcgacc ggccggggcc
     1321 ggtcgctttg ttggtgcttt tttcctctta ccccctcggg tcgcccgtta cttacggacg
     1381 agcagcagca gggggagaag aaagaacagg gacggaattc ccgcgacgct gcacccgcca
     1441 ccgccgcctg aaccgtcgtc cggcactgct gtccccaggg accgtgccag gttcagccgt
     1501 ccgccggtag agactttccc gctcagggag gagaggcggt ccaccccgcc caggatcctc
     1561 gagcggatct ggtccatgga ttctcccggg aaccgggacg acacaagggc cgccgctccc
     1621 gtcacgaagg gggtcgccat ggaggttccc gagatagcga tataggtatc cgtggaccag
     1681 cccactccca cgttgtcgat ctgtacccct gcgtagtgga acagaatgtc ggagtggtcc
     1741 gttttgaaga aaaacctgaa acggaagttc gccgtcctga attccgcggg aaccgggatc
     1801 gtaaagtccg tccagttgtc cccccggtaa attcgtgggc attcctccat gtggcgcctc
     1861 cgtccttgga aaactgcaga tagagatagt cgaatccgct ctccaggtcg caccgtatct
     1921 tcattccgaa gagcaccggc tgtcctgcgg ttcccgagag gtcgatgtcg ttctgtacgg
     1981 agagataggt cctctgattg atggggtagt gctggtccgg tccgtcggtc cagaccttcg
     2041 tgggccggtt gtccctcgtg gagtcgatga tcctccagtg gatttccggg gaagggtttt
     2101 ccacatgggt tgtccatttt ccggcacccg attccatgtc gtcgaagaag atgtccgatg
     2161 ccgatggctc gtacacccag gtgttcagga tggcggtccc cggtgcggcc aggtggacgg
     2221 acgttcttcc gtagttcgaa aaggaggaga gggcgtcatt cctgtcggtg gccgacacgg
     2281 agataatgtt cggcagggga taggacgagg gatagtgggg ggttccgtca ttgtcttctg
     2341 cagagtttcc ggcggcggcg atgaaaagga ttccggcgtt tccaagggct tcgatggcgt
     2401 ccctggcggc atcgctcgca ccgcctcctc cataggaggc gttcacggcc acgatgtcca
     2461 ccccttcctg tttcttccgc aggatccagt cgtaggcctc cagctcgttc tctgaagttc
     2521 ccgagccttg gtagtcgagc atcttcacgg ccatcagctt cacgttccac gccacaccgg
     2581 cggttccgat gccgttgttg cccttcgcgc cgatgatccc cgccacgtgg gtgccgtggc
     2641 cgttgtcgtc ctcgggatcg tcggtgtcgt ccacgcagtt ccggccgtgg atgcctccag
     2701 ggcccgccca gaggttgtcc ctgaggtcct cgtggagtga tgcgatgccg gtgtcgatga
     2761 tggccaccac cttttccggc cgggcagagg tgcggactcc ccaggctgcg ggagcgtcga
     2821 tgtcggcgcc tgccctgccg ccgtcctgcc ccgtgttatg gagcccccac tggctggaga
     2881 aaagggggtc gttggggatc acgggagaca gaatgcgcag gatgtagttc ggcgagacgc
     2941 tttccacggc gggatccgat gaaaatgtct cgagaagttc ctccgtcgat tttcccggaa
     3001 cccgaaggaa cacgacattt ttttcctgtt cagaggacag ggcctcgaac c
//
LOCUS       DAOTHD010000154         3047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_38075_length_3047_cov_3.148396, whole genome shotgun sequence.
ACCESSION   DAOTHD010000154 DAOTHD010000000
VERSION     DAOTHD010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38075_length_3047_cov_3.148396"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="P5219_03675"
     CDS             <1..204
                     /locus_tag="P5219_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRX25888.1"
                     /translation="EELGGASVHKATIVNVIVGTILFQGILTMTPSVINSMIQTDMSE
                     VIRIIVSNGMILYALTRVLKVKL"
     gene            201..1301
                     /locus_tag="P5219_03680"
     CDS             201..1301
                     /locus_tag="P5219_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRX25889.1"
                     /translation="MNRKYDNFKHILIQNAVPIVFLLVSAFAIPISQFSGTYLVQEML
                     VRLSRNSFLVLSLLIPIMAGMGLNFGMVLGAMAGQIGLIFITDWNVVGIYGMVLAAII
                     STPLAILLGWLCGLILNRARGREMVTSFILGFFMNGVYQLVVLYGFGSVVPVASQKMV
                     LSRGFGIRNVTNLEGVRKCLDNLLPVSVAGVEIPVATFLIIGLFCLFVLWFRKTKLGQ
                     DMRAVGQDLDVARDAGIPVERTRLISIVISTVLACYGQIIFLQNIGTMNTYNSHEQAG
                     MFAIASLLVGGSTVSKASIFNVFLGVVLFHLMFVVSPMAGKYLIGEAQLGEYFRVFVS
                     YGIISLALVLHAWRRQKEKDRTRRSLRGLQAE"
     gene            1313..1705
                     /locus_tag="P5219_03685"
     CDS             1313..1705
                     /locus_tag="P5219_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25890.1"
                     /translation="MKLKQILVNLAIIVFFITVAVFCYSEGKARNMLIENQSFSYEGK
                     TYDAFEAIEVTVDGQDPPLFLLEGDRGSVNLIGKSHVVVVEVLDENDKVIKTYSVSFK
                     SRELKGQIINVVPLVMEKLPGWNRELQK"
     gene            2045..>3047
                     /locus_tag="P5219_03690"
     CDS             2045..>3047
                     /locus_tag="P5219_03690"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRX25891.1"
                     /translation="MIRFVKTSVLASALLAAVFSSFAFAEQTSLEEFLLVVREKNPSL
                     QSSYKRLEALSHTVKSSVAVQRPSVGIRGSSSWLSDTYSSQAGRNMGEKGNYSLSAAI
                     THRFDVSGVYGAQERQLLLQYNGMASDHLTLVNNTLAAAESLYWQSFIARQNIILQRG
                     ILNQRKEDLRITEEKFRQQLIPRLDVVRAQAKVEEAESLVVEAESVYKNTLAGIRTLA
                     GGSDLEPREEDLLVPSLSVEVGIREAVERRNDVRSAETALERAKVLKTLAAKGMSPTV
                     EGSVGYMLLTDNDNASPVEREFLLALNVSIPVYDGGKTKEDVADKAKSVEAAEWSLDA
                     "
BASE COUNT          712 a          722 c          839 g          774 t
ORIGIN      
        1 gaggagctcg gcggcgcctc ggtccacaaa gcaacgatag tcaatgtaat tgtcggtacc
       61 atacttttcc agggaatcct cacaatgacc ccttccgtca ttaacagcat gattcagact
      121 gacatgtcag aggtgatcag gattatcgtt tccaacggaa tgatccttta tgctctcacg
      181 cgcgtattga aggtgaaatt atgaacagaa aatacgacaa tttcaagcat attctcatac
      241 agaacgccgt cccgatagtt ttccttctgg taagtgcttt tgcaattcct atctcgcagt
      301 tttccggaac gtaccttgtt caggaaatgc tcgtcaggtt atcgagaaat tctttcctcg
      361 tactttctct cctcattcct ataatggcag gcatggggct gaatttcgga atggttttgg
      421 gagcaatggc cggtcagatc gggcttatct ttatcactga ctggaatgtt gtcggaatct
      481 acggcatggt gctggctgcc attatttcca ctcctcttgc cattctgctg ggttggctgt
      541 gcggattgat actgaaccgg gcgaggggaa gggaaatggt gacatcgttc atcctgggat
      601 ttttcatgaa cggcgtctac cagcttgttg tcctgtacgg tttcggaagc gtcgttcccg
      661 ttgccagcca gaagatggtc ctttccaggg gctttggaat acggaacgtt acaaacctcg
      721 agggggtacg gaagtgtctc gacaacctcc tgccggtttc tgtggcaggt gtggaaatac
      781 cggtcgccac ttttctgatt attggactgt tctgcctttt tgttctttgg ttcagaaaga
      841 caaagctggg acaggatatg agggctgtgg ggcaggattt ggatgtagcc cgtgacgcgg
      901 gtatccccgt cgaaagaacg cgtcttatct ctatcgttat ttccaccgta ctcgcttgct
      961 atggacaaat aatcttcctt cagaatatcg gaaccatgaa tacatacaac agccatgaac
     1021 aggcaggcat gttcgcgata gcttctcttc tcgtaggtgg gtcgactgtc tcaaaggctt
     1081 cgattttcaa cgtctttctt ggtgtcgttc ttttccacct gatgttcgtt gtctctccca
     1141 tggccgggaa atatcttata ggtgaagccc agcttggaga gtatttcagg gtgttcgtct
     1201 cctacgggat tatttcccta gcgctggttc tccatgcctg gcgccgccaa aaagagaagg
     1261 acagaacaag gcggagtctt cggggactcc aggcggagta agggggatca tcatgaaact
     1321 gaagcagatc ctggtaaatt tggcgataat agtgttcttt ataacagtcg cggttttttg
     1381 ctacagtgag gggaaagcgc ggaatatgct cattgaaaac cagagttttt cttacgaagg
     1441 gaaaacatac gatgcctttg aagccatcga agtgactgtg gacgggcagg acccccctct
     1501 gttccttctt gagggtgaca gggggtctgt gaatctaatc ggcaagagcc atgttgtggt
     1561 cgttgaagtg cttgacgaaa atgacaaggt tatcaagacg tacagcgtct cttttaagag
     1621 cagggaattg aaggggcaga ttattaatgt tgtccccctc gtcatggaaa aacttccggg
     1681 ttggaaccgc gaactccaga aataaagtat attttccttc tgcaaaagaa gaacgtgttt
     1741 ttttatgaaa gggaacggca gctttttcct ggagaggctg tccgggaaga aaaatgtgat
     1801 gcccgtggga acatactgat cagaaccaat ggggaaagag cctctgctgt tttcccgttt
     1861 gaggtgtctg ttcccctggt tgtgaaccgc ggccgcccgg gttggccctg tttcttgacg
     1921 gagcggagga gaatatctat actcaaagga aaaaggatga acgggagaaa tgccttctcc
     1981 cgtgattttt ctgccttttt gccgccggca gatacaggac ggcaattccg gaggaggaac
     2041 acctatgata cggttcgtaa aaacttctgt tttagcttca gcgttgctgg ccgccgtttt
     2101 ctcttctttt gcgttcgcgg agcagacgtc cctggaagaa ttccttcttg tggtgcgtga
     2161 aaaaaatccg tcccttcagt cttcatacaa acggctggaa gccctcagtc atacagtgaa
     2221 gtcctcggtg gcagtccagc gcccgtcagt gggtatccgg ggcagcagct cctggctcag
     2281 tgatacctac tcctcccagg ccggaaggaa tatgggggag aagggcaatt attccctctc
     2341 tgcggcgata acccaccgtt tcgacgtgag cggggtctac ggggcccagg agcggcagct
     2401 tcttctccag tacaacggta tggcatccga ccatctcacc ctggtgaata acactcttgc
     2461 cgcagcggaa agcctgtact ggcagtcctt tattgcccgt caaaacatta tcctccagag
     2521 gggcatcctg aaccagcgga aagaagacct gagaattacc gaggaaaagt tcaggcagca
     2581 gctcatcccc cggctggacg tagtacgggc ccaggcaaag gtggaggagg ccgaaagcct
     2641 tgtggtggaa gcagagtctg tctataaaaa tacacttgcc ggaataagaa cccttgctgg
     2701 gggttcggat ctggaaccgc gggaggaaga ccttctcgta ccgtccctgt ctgttgaggt
     2761 cgggatacgg gaggctgtcg aacggaggaa cgacgtccgg tccgcagaga cggccctgga
     2821 gcgggcaaaa gttctgaaga ccctcgccgc gaaggggatg tctcccacgg ttgaaggctc
     2881 ggtgggatat atgctcctga cggacaatga caatgcctct cctgtagaaa gggaattcct
     2941 ccttgccctg aatgtttcca ttcccgtata cgacggaggg aaaacgaaag aagacgtggc
     3001 ggacaaggcc aaaagcgtcg aggctgcgga gtggagcctc gacgccc
//
LOCUS       DAOTHD010000155         3032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_38315_length_3032_cov_4.199194, whole genome shotgun sequence.
ACCESSION   DAOTHD010000155 DAOTHD010000000
VERSION     DAOTHD010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38315_length_3032_cov_4.199194"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="P5219_03695"
     CDS             complement(<1..288)
                     /locus_tag="P5219_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRX25892.1"
                     /translation="MGAKTARRMAFFVLQQPVSYAEEFAACLSGLKDRIFTCSRCGNI
                     TDREPCSVCSDLLRDRSTLCVVETVEDLISIEQAGVYSGLYHVLGGRVSPLD"
     gene            complement(355..657)
                     /locus_tag="P5219_03700"
     CDS             complement(355..657)
                     /locus_tag="P5219_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010330208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRX25893.1"
                     /translation="MDKIMKQAQKMQAQMAKVQEDLAGEKVEGSAGGGMVTVTANGQG
                     DVVAVRIDPEVMKDDDVEMLEDLVLAAVNEALRRSKELAAERMGKITGGLGSMGLF"
     gene            complement(700..1161)
                     /locus_tag="P5219_03705"
     CDS             complement(700..1161)
                     /locus_tag="P5219_03705"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HRX25894.1"
                     /translation="MKIIILSVGKTRDGHLAALADRYFQRISPFMKAGLEIAPESKDG
                     NAARKVEREGKEILKKIRERDFVVVLDEGGGETDSLSFARWLSEMLDGTDGRIVFVIG
                     GAFGLSGEVRDRANHFLSLSKMTMPHELCRVFLLEQVYRACTILRGVDYHH"
     gene            complement(1142..1834)
                     /locus_tag="P5219_03710"
     CDS             complement(1142..1834)
                     /locus_tag="P5219_03710"
                     /inference="COORDINATES: protein motif:HMM:NF013935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HRX25895.1"
                     /translation="MNGRTSGPPTREHLLSTRRRIVAVSHGKRLLERKRDALVRAVDE
                     ERRLFRETEKDFLLAAKRITFLYSLVRLFEGDGAVRYLSAGNSPLPVGVEKKSIMGCR
                     YSSFFPSPEGKERLSWKLPFDPAVTSLSVEDLLKALGESEECVWRYINLKTKIGAIER
                     ELSRTTLKINTLEHVVLPSLGKEVKRIEDLLAERERQEKFVSKRVGKKKRSASAGNRE
                     NMLDTNYENHYS"
     gene            complement(1831..>3032)
                     /locus_tag="P5219_03715"
     CDS             complement(1831..>3032)
                     /locus_tag="P5219_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HRX25896.1"
                     /translation="APGSVTVWLERDVLRIPAGDGLVGRVFDGRGNPLDGLPAGFADE
                     FIAVGGSPINPVRRSSPDIFVETGVSALDLMNTLVRGQKLPVFSGAGLPANELAVQIV
                     SGARIPGSEEPFLVVFAAMGITRREAQFFMNSFRESGALRNGVFFLNLAGDSTVERLL
                     TPRVALAAAEYFAFSRGYNVLVVMTDMLAYCDALREVAAAREEVPGRRGYPGYMYSDL
                     AELYERAGCIAGAPGSVTQIPIITMPDDDMTHPVVDLSGYITEGQIVLDRRIQERGVY
                     PPIDVLPCLSRLMNKGIGRGKTFQEHRALADQLYASYARAREVERMRLIVGDDGLTAV
                     EKQYLGFGTAFEKSFLGQGGERRTLAESERCAWKCLETLPRSELFKLPESYVVESLGY
                     SALPREQ"
BASE COUNT          615 a          956 c          795 g          666 t
ORIGIN      
        1 gtcaagggga gacactctcc ccccgaggac atggtacagg cccgaataca cgcccgcctg
       61 ttcgatgctg ataagatcct ccaccgtttc cacgacgcag agggtcgacc tgtcccgcag
      121 aagatcgctg cagaccgaac agggttccct gtcggtgatg ttgccgcacc gggaacaggt
      181 aaatatgcgg tctttcagtc ctgaaagaca cgctgcgaac tcttcggcat aggaaacggg
      241 ctgctggagg acaaaaaaag ccatccgccg ggccgtcttc gcccccacgc ccggaagttt
      301 ccgaaaaagt acagtcagcc tttcaaatga atcaggcagc gacacgaaga acctctaaaa
      361 gagccccatg gaacccagtc cgccggtaat cttccccatt ctttcggcgg cgagttcctt
      421 gctgcggcgc agagcttcgt tcaccgccgc aagcaccagg tcctcgagca tttccacatc
      481 atcgtccttc ataacctccg ggtcgatgcg gaccgcgacc acatctccct gaccattggc
      541 cgtcacggtg accattccgc ctccggcgct cccttccacc ttttccccgg cgaggtcctc
      601 ctggaccttt gccatctggg cctgcatttt ctgcgcctgt ttcatgatct tgtccatgtt
      661 caagcaaacc actccttttc tctgatatcc ccaaaaatct cagtgatggt agtcaactcc
      721 cctgagtatg gtgcaggccc ggtaaacctg ctccagaaga aagacacggc ataactcgtg
      781 gggcatggtc atttttgaca gggagagaaa atggttcgcc ctgtcccgta cttccccgga
      841 aagtccgaat gccccgccga tgacaaaaac aatccttccg tcagttccgt caagcatttc
      901 cgagagccac cgggcaaaag agaggctgtc cgtttcaccg cctccctcgt caagaaccac
      961 tacaaaatcc cgttcccgga tcttcttcag aatctccttt ccttcccgct ccaccttccg
     1021 ggcggcattg ccgtccttcg actcaggcgc gatttcaaga cccgctttca taaaaggaga
     1081 tatccgctga aaataacgat ctgccagggc ggccaaatgg ccgtctcgtg tttttccgac
     1141 actaagaata atgattttca tagtttgtgt ccagcatatt ttccctattt cccgccgaag
     1201 ccgaccgttt tttctttccc acccttttcg aaacgaattt ttcctgccgt tccctctcgg
     1261 cgagaaggtc ctctatccgc ttgacctctt tccccaggga gggaaggacc acgtgttcaa
     1321 gggtatttat cttcagggtg gtcctggaaa gctcccgttc gatggccccg atcttcgtct
     1381 tgaggttgat gtatctccag acgcattctt cgctttctcc cagggctttc agaaggtctt
     1441 ccaccgaaag ggaggtcacg gcaggatcga agggaagctt ccatgaaagg cgctcctttc
     1501 cctccggcga agggaaaaaa gaggaatacc ggcatcccat gatggatttc ttttccaccc
     1561 ctaccggcag ggggctattt cccgcagaaa ggtagcggac tgcgccgtcg ccctcaaaga
     1621 gcctcaccag ggaataaagg aaggtgatcc tcttcgcggc gagaaggaag tccttttctg
     1681 tctcccggaa gagacgccgc tcttcgtcca cggcccgtac cagggcgtcc cgtttccgtt
     1741 cgagaagcct tttcccgtgg ctcaccgcga caatgcgccg gcgggtggac agaaggtgtt
     1801 cccgtgtggg agggccggag gtcctgccgt tcattgctcc cggggaaggg cggaatagcc
     1861 caggctttcc accacgtagc tttcaggaag cttgaagagt tcactccttg ggagtgtctc
     1921 caggcatttc caggcgcagc gctccgattc ggccagggtt ctgcgttcgc ctccctggcc
     1981 gaggaaggat ttctcgaagg ccgttccgaa cccgaggtat tgtttttcca ctgctgtgag
     2041 accgtcgtcg cctacgatga gccgcatccg ctccacttcc ctggccctgg cgtaggaggc
     2101 gtagagctgg tcggcgaggg ccctgtgttc ctggaaggtt tttccccttc ctatcccctt
     2161 gttcatcagt ctgctcaggc agggaaggac atcgatgggg ggatagactc ccctttcctg
     2221 gatcctccgg tcaagcacga tctgcccctc ggtgatatac cccgagaggt ccaccaccgg
     2281 atgggtcatg tcgtcgtccg gcatggtgat gatgggaatc tgggtgacgc ttcccggcgc
     2341 tcccgcgata cagcccgccc gttcgtacag ctctgccaaa tcggaataca tatacccggg
     2401 gtatcctctc ctccccggaa cctcttccct ggcggccgcc acttccctca gggcgtcgca
     2461 gtaggccagc atgtcggtca ttaccacgag gacgttgtat cccctggaaa atgcgaagta
     2521 ttccgccgcc gcaagggcaa cccggggtgt cagcagcctc tcgaccgtcg aatctcctgc
     2581 gaggttcaga aagaacacgc cgttcctgag ggcgcccgac tcccggaaag aattcatgaa
     2641 aaactgggct tccctccgtg taatgcccat ggcagcgaag accacgagaa aaggttcctc
     2701 cgatccgggg atccttgcgc cgctcacgat ctggactgcc agttcgtttg cgggaaggcc
     2761 ggctccggag aagacgggca gtttctgtcc acggacgagg gtgttcatga ggtccagggc
     2821 cgacactccc gtttccacga aaatatcagg actgctcctc ctcacaggat tgatgggcga
     2881 acctcccaca gcgataaatt catcggcgaa gcccgcaggc agaccgtcca ggggatttcc
     2941 tctcccgtcg aagacacggc cgaccagtcc gtctccggcg ggaatcctga ggacgtcccg
     3001 ttcaagccac accgtaacgc ttccgggggc aa
//
LOCUS       DAOTHD010000156         3027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_38407_length_3027_cov_2.679004, whole genome shotgun sequence.
ACCESSION   DAOTHD010000156 DAOTHD010000000
VERSION     DAOTHD010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38407_length_3027_cov_2.679004"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="P5219_03720"
     CDS             complement(<1..1225)
                     /locus_tag="P5219_03720"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRX25897.1"
                     /translation="MARKRYQHELELRQFTLEDYDEVKRIMDRIYPKMGGAWTRDQFR
                     AMITRFPEGQLCIEDKGRVVAGAFSLVIDYSRFGDRHTYQEVVGDGTLKNHDPEGDYL
                     YGIDIFVSPDYREKRLGRRLYDARKELCETLNLKGIVIGGRIPGYAEWAEKKTPQQYI
                     RMVNNREISDPVLTFQVSNDFHYKKIVENYWPEDYSSKGYGVIMEWNNIYYEERKRLI
                     SKVKTYMRLGVVQWQMRSFESFDDFLQQVEFFVDTQAGYNTDLMLFPELFNAPLLAQF
                     DQEDPAEAMRSLAGYTAELRKSLVEMAVSYNINIVSGSVPELRNNRLYNVSFLCRRDG
                     TWDQQYKLHITPDEYRYWGLQGGHDLRVFQTDVGKVGILICYDVEFPELSRYLAIKGM
                     DILLIPFWTDTKNAYL"
     gene            complement(1355..1747)
                     /locus_tag="P5219_03725"
     CDS             complement(1355..1747)
                     /locus_tag="P5219_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRX25898.1"
                     /translation="MAKPEMEFTDTLPLGQWRQVEGAEKGVFEKILSWDPDTGNLTRL
                     VKFGSGVRIPKTQVHDFCEEVFVLEGYMVDTEKKLTAAPGYYACRPVGMVHGPYEIPV
                     SCLCFETRYQDPARPLNPDCSLYGMKPE"
     gene            complement(1770..>3027)
                     /locus_tag="P5219_03730"
     CDS             complement(1770..>3027)
                     /locus_tag="P5219_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRX25899.1"
                     /translation="GLFGVFLLGIPISFSLGATTLAGLLGMGLPLEVVVQRMFTGVDN
                     FAFVAIPLFILAGNLMAVGGISKRITTFSEALVGHWPGGLGMVAIVASMIFAAVTGSA
                     IAATAAIGALLISEMISKGYSRAYSASLVATAGSIGPIIPPSIPLVVYGVIVSSSIAK
                     LFMGGVIPGLLMGFFLMVANWLISKRRGYVGREKRASAAELRQGLKDAVLALLMPVII
                     IGGIVAGIFTPTESAAIAVAYALLLGWLVYRELTWKKVVHAFIEAAITNGVILTVLMT
                     ASLFIWFMTVKMIPQSITQSLLQVIDTKWGALMVMNIILLIAGTFIDTTSALTIFVPL
                     FLPLVKTFGIDLIHFGVMVAVNLTIGMCTPPLGVCLFVSCEIAGTSLREMFRDLVIML
                     IPLLILLLLITYVPATVTWLPDFLGL"
BASE COUNT          627 a          886 c          923 g          591 t
ORIGIN      
        1 ggaggtaggc attctttgtg tccgtccaga aggggatgag caggatgtcc atgcccttga
       61 tggccaggta ccgggagagt tcggggaatt caacgtcata gcatatgagg atgcccacct
      121 tccccacgtc cgtctggaag acccggagat cgtgccctcc ctggaggccc cagtaccggt
      181 actcgtccgg ggtgatgtgg agcttgtact gctggtccca ggtgccgtcg cggcgacaga
      241 gaaaggaaac gttgtagagc ctgttgttcc ggagctccgg cacgcttccc gacacgatgt
      301 tgatgttgta ggacaccgcc atctccacca ggctcttccg gagttccgcc gtatacccgg
      361 ccagggaccg catggcctcc gccgggtctt cctggtcgaa ctgggccagc agaggggcgt
      421 tgaacagttc ggggaagagc atgaggtccg tattgtaccc cgcctgggtg tccacgaaga
      481 actccacctg ctgcagaaag tcgtcgaagg actcgaagct cctcatctgc cactgaacca
      541 cgcccaggcg catgtaggtc ttcaccttcg agatgagccg cttccgctcc tcgtagtaga
      601 tgttgttcca ctccatgatg acgccgtacc ccttggagct gtagtcctcg ggccagtaat
      661 tctccacgat cttcttgtaa tggaagtcgt tgctgacctg gaaggtgagg acggggtcgg
      721 agatctcccg gttgttcacc atgcggatgt attgctgggg ggtcttcttc tccgcccatt
      781 cggcatagcc gggaatccgg ccgccgataa cgatcccctt gaggttcagg gtctcgcaga
      841 gctccttcct ggcgtcgtag agacgcctgc cgagccgctt ttccctgtag tcgggcgaca
      901 cgaaaatgtc gatgccgtag agataatccc cttcggggtc gtggttcttc agggtgccgt
      961 cgccgacgac ttcctggtag gtgtgcctgt cgccgaaacg ggaatagtcg atgacgagag
     1021 aaaaagcacc ggccaccaca cgccccttgt cctcgatgca gagctgcccc tcgggaaagc
     1081 gggtgatcat cgcccggaac tggtccctgg tccaggctcc tcccattttc gggtatatcc
     1141 tgtccatgat ccgtttgact tcatcataat cttcaagagt gaactggcgc agctccagct
     1201 cgtgctggta ccgtttccgg gccataagcg tccgtccttt caatgcagaa tttcacgtat
     1261 tcctattata cggtgcgccg ggaagaaaaa catgcgtatg gacgaacccg gaaaaaacaa
     1321 ccgccccgaa aaaaatccgg ggcggttcgg ccggttattc cggtttcatt ccgtagagag
     1381 agcagtccgg attcagcggg cgggccggat cctggtagcg ggtctcgaag caaaggcacg
     1441 agaccggaat ctcgtacggc ccgtggacca tgcccaccgg cctgcaggcg taataccccg
     1501 gcgcggcggt cagctttttt tccgtgtcca ccatgtatcc ttcgaggacg aacacttcct
     1561 cgcagaaatc gtggacctgg gtcttcggga tccgcacccc cgagccgaac ttcaccagcc
     1621 gggtgaggtt tcccgtgtca ggatcccagg agaggatctt ttcgaagacc cccttttccg
     1681 ctccctccac ctgcctccac tgtcccaggg gcagggtgtc ggtaaattcc atctccggtt
     1741 ttgccatgac aatcctcctt agggtcgaat cagagcccga ggaaatcggg cagccaggtc
     1801 accgttgccg gtacgtaggt tatgagcagc agcagaatca gcaggggtat gagcatgatc
     1861 accaggtccc tgaacatctc ccgcagggaa gtgccggcga tctcgcagga gacgaagagg
     1921 cagaccccaa gaggcggggt gcacatgccg atggtcaggt tgaccgccac catgacgccg
     1981 aaatggatca ggtcgatgcc gaaggtcttc accaggggaa ggaacagggg gacgaagatg
     2041 gtgagggcgc tggtggtgtc gatgaaggtc ccggcgatga gcaggatgat gttcatcacc
     2101 atcagggctc cccactttgt gtcgatgacc tggagcaggc tctgggtgat ggactggggg
     2161 atcatcttca ccgtcatgaa ccagataaac aggctcgccg tcatgagcac cgtgaggatg
     2221 accccgttcg ttatggccgc ctcgatgaag gcgtggacca cctttttcca ggtcagttcc
     2281 cggtagacca gccagccgag gagcagggcg taggcgaccg cgatggcggc cgactcggtg
     2341 ggggtaaaga tcccagccac aatgccgccg atgatgatca ccggcatcag aagggcaaga
     2401 acggcatcct tgagaccctg gcgcagttca gccgctgagg ccctcttttc ccgtcccaca
     2461 tatcccctcc gtttgctgat gagccagttg gccaccatga ggaaaaagcc catgagaaga
     2521 ccggggatga cgccccccat gaacagcttc gcgatggagc tgctcacgat gacgccgtac
     2581 accaccaggg gaatgctcgg cgggatgatg gggccgatgg atcccgccgt tgcgacgagg
     2641 gatgccgaat aagcccggga atatcccttg gagatcatct cgctgatgag cagtgcaccg
     2701 atggccgccg tggcagcaat ggccgatccc gtgacggcgg cgaagatcat ggaggccacg
     2761 atggccacca tgccgagacc gccgggccag tgcccgacca gggcttctga aaaggtggtg
     2821 atccgttttg aaatgccccc cacggccatc aggttgcccg cgagaataaa cagggggatg
     2881 gccacgaaag caaagttgtc tacccccgtg aacatccgct ggacgacgac ttcgagggga
     2941 agtcccatgc cgagcagccc cgcaagggtc gtggccccga gggagaagga tatggggatt
     3001 cccagcagga aaacgccgaa caggccg
//
LOCUS       DAOTHD010000157         3020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_38533_length_3020_cov_3.273862, whole genome shotgun sequence.
ACCESSION   DAOTHD010000157 DAOTHD010000000
VERSION     DAOTHD010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38533_length_3020_cov_3.273862"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="P5219_03735"
     CDS             complement(<1..700)
                     /locus_tag="P5219_03735"
                     /inference="COORDINATES: protein motif:HMM:NF018037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1036 domain-containing protein"
                     /protein_id="HRX25900.1"
                     /translation="MPIGLRSLAVLCLTALFLMGPPPAAFALKLTIRNDFADSLNTAV
                     VYYCDSTGAWTTRGWYVVRPRESRTITFSTSKPAMYLHSYLSGRNTISWGKGDITRVV
                     MSKAFVYEDGETCPAGPGRRTAKFTRYAAKNGRVNYSPVKTGEPLPAGGGDTFSAVRN
                     ELLKLINADRRKTGARDLKLDATLSKAAARRASEQPKVWGHTRPNGKSYSSVFAEFNL
                     NPARSGENVASNTKP"
     gene            complement(791..1336)
                     /locus_tag="P5219_03740"
     CDS             complement(791..1336)
                     /locus_tag="P5219_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25901.1"
                     /translation="MRGKNRFGLLAGMAVLLVLVFASAALAKPDWVTVRYTGCKLSTE
                     KNTKVTDGNHRKLVLYFDVINNSKSGDIITAIYDRKIGYKGVFTIHKMEQGLVQNGVI
                     QSQGWRPATWNVSYSGTYTKPIKGEWYPGQVYKYDISVSLNKLIKPFGGNWKKTNEAA
                     SRGFNFKPNTLSFDFGVRSHK"
     gene            complement(1495..2766)
                     /locus_tag="P5219_03745"
     CDS             complement(1495..2766)
                     /locus_tag="P5219_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25902.1"
                     /translation="MRKVMLYVSLFCVFAAAAFCFGTAANADDSPDGWVKGLWIQAAG
                     LPKDAVIEDFSDGDDEKPWFVYSWGKGSDGPAVTLGVGRFPASADIGEKLLNLDKKVL
                     REFIGSEAFWEGPDAKKLTFTQAPEKISAGLSYPCQIVRYAESDMGLSHMVLFIETDP
                     FLFMAEITWETENKKFKKSDGEKILANLALVEQEGMKASGPDGDLFSLKDGRLFMGEE
                     SVEAEVNEVPPEIEGPVRFWSAVGEDDSGLVRENETGVRFFAEDGAFLAFLPLESADE
                     CQDIIFSPDGSFFLLMSGSGIRADMTYILYDPETMEKKIEISGTRGSAEWIDVGRFVM
                     TRIDDVRETESGNFSMAALRLSAVMYDTVAEELVVLKEGTATKNYWCGGLADDGGSLI
                     ISEDSVTSEKDWGDEEKITTREIKIEIPAAG"
BASE COUNT          582 a          918 c          754 g          766 t
ORIGIN      
        1 gaggtttcgt atttgaggcc acgttttcac cgcttcttgc cggattgaga ttgaactcgg
       61 cgaagacgga gctgtagctt tttccgttcg gacgggtgtg gccccacacc ttcggctgtt
      121 ccgaagcccg gcgggccgcc gccttcgaca gggtcgcatc cagtttcaga tcccgggcgc
      181 cggtctttct gcggtcggca ttgatgagtt tcagcagttc attgcgtacc gccgaaaaag
      241 tgtctcctcc ccctgcggga aggggctccc ccgtcttcac cggactgtag ttcacacggc
      301 cgttttttgc cgcgtacctg gtgaacttcg ctgtcctcct gccgggaccg gcagggcatg
      361 tttcgccgtc ctcgtacaca aaggccttcg acatgaccac ccttgtaatg tccccttttc
      421 cccaggagat tgtattcctg ccggagagat aggaatggag atacatggcc ggtttggagg
      481 tggaaaaggt gatggtccgc gattcgcggg gcctcaccac gtaccagcct ctggtcgtcc
      541 atgcgccggt ggagtcgcag tagtagacca cggctgtgtt gaggctgtct gcgaagtcgt
      601 ttcggatcgt cagtttcagg gcaaaggccg ccggcggggg tcccatcagg aaaagagcgg
      661 taagacacag gaccgccagg cttcgcagcc ctatcggcat ttttttcaga acggcctcct
      721 cctttttcgc acctcgaaag ctccctccgc acagcttcaa aagccggcgc tttttgcatg
      781 aacagcggcc ttacttgtgg ctgcggaccc cgaaatcaaa agacagtgta ttcggcttga
      841 aattaaagcc tctggacgcc gcttcattgg tctttttcca gttgcccccg aaaggcttga
      901 tgagtttatt gagggaaacg gagatgtcgt acttgtacac ctgtccggga taccattctc
      961 cttttatcgg tttggtatat gtgccggaat aactgacgtt ccacgttgca ggtctccatc
     1021 cctgcgactg tatcacgccg ttctggacaa ggccctgttc catcttgtgg atggtgaaca
     1081 ctcctttgta gcctattttt ctgtcgtaga tggccgtaat gatatcgccg ctcttgctgt
     1141 tgttgatgac gtcgaagtac agaaccagtt ttctgtggtt gccgtccgtc accttcgtgt
     1201 tcttttccgt cgaaagtttg catcccgtgt acctgacagt cacccagtcg ggtttcgcca
     1261 gggctgcgga ggcgaacacc agcacaagca gcaccgccat gcccgccagc agtccgaacc
     1321 tgttctttcc tctcatgtct tgcccccctt tgttgaatat gctttccgcc cttctctgaa
     1381 acagaacacc gccgattgct aacaggcgga atctgaaaca gcaccctgat tgtgctgcgg
     1441 gagtctcctc tcccgcagca caatcgcccc acgacacagg tccgcggcag gtctttaccc
     1501 tgccgcggga atctctattt ttatttcccg cgttgtgatt ttttcctcgt cgccccagtc
     1561 cttttccgac gtcactgaat cttcgctgat gatgagcgag ccgccgtcgt ccgccaaacc
     1621 gccgcaccag taatttttcg tcgcggtccc ttctttcaga acgaccagct cctccgccac
     1681 ggtgtcatac atcactgccg agaggcggag cgccgccatg gaaaaattcc cgctttccgt
     1741 ctcgcgcaca tcgtcgatcc gggtcatcac aaaacggccg acgtcaatcc actctgcgga
     1801 tcctcttgtc ccggaaattt cgattttttt ctccattgtt tccggatcgt agagaatgta
     1861 cgtcatgtcg gcccggatcc cgcttccgct catcagcagg aaaaaactcc cgtccggact
     1921 gaaaatgata tcctggcact cgtccgcgct ttcgaggggc aggaacgcca gaaaggctcc
     1981 gtcttccgca aagaaccgga cgccggtttc attttccctg acgaggcccg agtcatcctc
     2041 cccgacggcg ctccagaaac gtaccgggcc ttcgatttcc ggcggtacct cgttcacttc
     2101 cgcttcaaca ctttcctcgc ccataaaaag gcgcccgtcc ttcagtgaaa ataggtcacc
     2161 gtccggcccg gaggctttca tcccttcctg ctcgacgaga gcaagattcg ccaggatttt
     2221 ttccccgtcg ctttttttga actttttgtt ctctgtttcc caggttatct cagccataaa
     2281 aagaaacgga tcggtctcta tgaacaggac catgtgggag agccccatat ccgattccgc
     2341 gtatcgcacg atctggcagg ggtaggagag gcccgcgctt attttttcag gggcctgcgt
     2401 gaatgtcagc tttttcgcgt cagggccttc ccagaacgct tccgagccga tgaattcgcg
     2461 aagcactttt ttgtcgaggt tcagcagttt ttccccgata tccgcagaag ctggaaagcg
     2521 cccaacgccg agcgtcacgg cagggccgtc gctgcccttt ccccaactgt agacaaacca
     2581 tggcttttca tcgtcgccgt cggaaaaatc ttcgatcacc gcgtctttgg gcagcccggc
     2641 cgcttgtatc cacagccctt tcacccaccc atccggtgaa tcgtctgcgt tcgccgcagt
     2701 tccgaagcag aatgccgcag cggcaaaaac acagaaaagc gaaacgtaca gcatgacctt
     2761 tctcatttca ctgaaacctc ctgtctctgc atggttccgg acctgaaaac cggggttccc
     2821 tccgccgaag gtgaagcgaa catcaaaatc gctgccgaac aggtattcac ctcgttttct
     2881 gtacctcttt tttcccggcg ctttttttct attgtttaaa ttgctccggg tcgacgttga
     2941 gaatcaggca cgccgtttcc cacacgctca gatccttgcg ccagtcgtaa aaattattca
     3001 tcatctgggc gaaggcatgt
//
LOCUS       DAOTHD010000158         3000 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_38879_length_3000_cov_4.109677, whole genome shotgun sequence.
ACCESSION   DAOTHD010000158 DAOTHD010000000
VERSION     DAOTHD010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38879_length_3000_cov_4.109677"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..1123)
                     /locus_tag="P5219_03750"
     CDS             complement(152..1123)
                     /locus_tag="P5219_03750"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HRX25903.1"
                     /translation="MKTITFSQATLEAMQEEMARDERVFVMGEDIARQGGIFGQFKGL
                     PAQFGTGRIMDTPISETAIVGACIGAALAGMRPVADMHFADFAGVCFDEIYNQMAKIY
                     YMFGGQKPVSMVLRAPDGLVNQAAAQHSQSLEAFFCHIPGLKVVIPSNPADAKGLLKA
                     AIRDDNPVIYFEHKALFSMKGEVPEEEYVTPIGKAKVVREGKDVTLVSYSMTMNLAVK
                     AADMLEKEGISVELIDLRTISPIDKETILNSVAKTSRLAIAHEAVKQGGVGAEISAIV
                     AEEAIDCLDAPIVRVGAPFTPVPFARPLEQAYRITPDKIAAAVRGMM"
     gene            complement(1137..2132)
                     /locus_tag="P5219_03755"
     CDS             complement(1137..2132)
                     /locus_tag="P5219_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HRX25904.1"
                     /translation="MPVRKEATLPLARYDKETLKFFYETMVKIRNFELTVEKFFFDGE
                     IPGFVHLYIGEEATATGVMANLRKTDYIQSTHRGHGHTIAKGADLKHMMAELFGKSTG
                     SCKGKGGSMHIADFSVGMLGANGIVGGGFTMATGAALAQKMQKTDGVAVAFFGDGASN
                     RGTFHEAANMAAAWKLPVIFLCENNQWASTTPYRTTTSVDDIADRAQGYGMPGVIVDG
                     NDVFAVYEAAKELIERARRGEGPAILECKTYRIKGHFVGDPEMYRTKEEVQKVLEETD
                     PIPRFEAKVLKAKAMTKKELETIRTRVEKELAEALEFARTSPEPEVSALYEGLYV"
     gene            complement(2324..2977)
                     /locus_tag="P5219_03760"
     CDS             complement(2324..2977)
                     /locus_tag="P5219_03760"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRX25905.1"
                     /translation="MRKKKKDQAYEEIRKLVLQRRSTDRFSFSENSLAAEMRMSRTPI
                     REALQRLQMEGFIEIHPNRGVVIPEVSVVEVNETFALRMAVEEFVIREMTPQMTPENI
                     GEMDLYLSRQKEAMERNDVLEYLRHDKDFHDLFFRLYANSLIFNTIQRVLDRFYSMGT
                     NVLRRPGSVQRSFAEHCAVADAVRNGDGEKAASAMHAHLITGKNNVLSIPGNIESVV"
BASE COUNT          541 a          899 c          915 g          645 t
ORIGIN      
        1 gaggcccgat gccacggaga cagtggcggg tgcagattac cctgacggcg gcaaaaaggt
       61 cctctcccgg ggcgattctc ccgagaaccg tcgccgagat ggtgcccggg gccgaggaga
      121 ctctctccgc tgacacgttc gtttccccct cttacatcat tccccggacc gccgcagcga
      181 tcttgtcggg ggtgatgcgg taggcctgct ccagcggccg ggcaaagggc accggggtga
      241 agggagcgcc cacccggacg atgggggcgt cgaggcagtc gatggcctct tcggcgacaa
      301 tggccgatat ttcggcgccc acgccgccct gcttcaccgc ttcgtgggcg atggcgagcc
      361 gggacgtctt tgccacagaa ttgaggatcg tctctttatc gatgggagaa atggtcctca
      421 ggtcgatgag ctccacgctg atcccctctt tttcaagcat gtcggccgcc ttgaccgcaa
      481 gattcatggt catggaatag gacacgaggg tgacatcttt gccttcccgg accaccttcg
      541 ccttgccgat gggagtgaca tactcctcct cgggaacctc ccccttcatg gagaagagag
      601 ccttgtgctc gaagtagatg acggggttgt cgtccctgat ggctgccttc aggagcccct
      661 tggcatccgc cgggttggag gggatgacca ctttcaggcc gggaatgtgg cagaaaaagg
      721 cctcgaggga ctgggagtgc tgggcagcgg cctggttcac gaggccgtcc ggggcccgga
      781 gcaccatgga gacgggcttc tggccgccga acatgtagta gatcttggcc atctggttgt
      841 aaatctcgtc gaagcagacc ccggcaaagt cggcgaagtg catgtccgcc acgggacgca
      901 ttcccgcgag agcggcgccg atgcaggcac cgacgatggc cgtctcgctg atgggggtgt
      961 ccatgatccg tccggttccg aactgggcgg gaaggccctt gaactggccg aaaattccgc
     1021 cctggcgggc gatgtcctcg cccatgacga acacccgttc gtcccgggcc atctcctcct
     1081 gcatggcttc aagggttgcc tgggaaaaag tgattgtctt cattctgtcc accgccttac
     1141 acatagagcc cttcatacag ggcagacact tcgggttccg gagaggtacg ggcgaattcc
     1201 agcgcctcgg caagctcctt ctccaccctg gtcctgatgg tctcgagctc ttttttcgtc
     1261 atggccttgg ctttgagaac cttggcctcg aaccggggga tggggtccgt ctcttcgagg
     1321 accttctgga cctcttcctt ggtgcggtac atctcggggt cgccaacgaa atggcccttg
     1381 atccgatagg tcttgcactc gaggatcgca ggtccttctc ccctgcgggc ccgctcgatg
     1441 agctccttcg cggcctcgta cacggcgaac acgtcgttgc cgtccacgat cacgccgggc
     1501 ataccgtagc cctgggcccg gtcggcgatg tcgtccaccg aggtggtggt ccggtagggg
     1561 gtggtggagg cccactggtt gttctcgcag aggaagatca cgggaagttt ccatgccgcg
     1621 gccatgttgg cggcctcgtg gaaggttccc ctgttggagg cgccgtcgcc gaaaaaggcc
     1681 acggcgacgc cgtcggtctt ctgcatcttc tgggcgagcg ccgcgccggt ggccatggtg
     1741 aaaccgccgc ccacgatgcc gttggcgccc agcatgccca cgctgaagtc ggcgatgtgc
     1801 atggagccgc ccttgccctt gcagcttccc gtgctctttc cgaacagctc ggccatcatg
     1861 tgcttcaggt cggcgccctt ggcgatggtg tggccgtgcc ccctgtgggt gctctggata
     1921 tagtcggtct ttctcaggtt cgccatgacg cccgtggcag tggcttcctc gccgatgtac
     1981 aggtggacga acccggggat ctctccgtca aagaaaaatt tctccacggt gagttcgaaa
     2041 ttgcggatct tcaccatggt ctcatagaaa aacttcagcg tttctttatc gtaccgtgca
     2101 agaggaagag tcgcctcttt ccgtaccggc attgcacaca tgaagatcac ctcgtcagaa
     2161 ttctggtctt ggttcctctg cccccggcgg acaggctccg ccgcgcgccg ctcaccgcag
     2221 gaatacggag cggcgaaaag cacccccctt tctgaagctg aacccggaat tgaaatgcta
     2281 tcagtagaaa attcgatgcc acagcgttaa aacaacggga ccgtcacacg acgctctcga
     2341 tatttcccgg aatggaaaga acattgtttt ttcccgtgat caggtgggca tgcatggccg
     2401 aggcagcctt ttcaccgtcg ccgttccgaa cggcatcggc gacggcacag tgttccgcga
     2461 aggagcgctg gacgctcccc ggccgcctca gcacgttggt tcccatggag taaaaccggt
     2521 cgaggacacg ctgtatggtg ttgaaaatca gggaattggc gtataaacgg aagaagaggt
     2581 catgaaaatc cttgtcgtgc ctgaggtatt ccaggacatc gttccgttcc atggcttcct
     2641 tctggcggga aagatagagg tccatctcgc cgatgttttc cggggtcatc tggggggtca
     2701 tctcccggat gacgaattcc tccacggcca tccggagggc gaaggtctcg ttcacttcca
     2761 ccacggaaac ttcggggata accacgcccc ggttcgggtg aatctcgatg aagccttcca
     2821 tctgaaggcg ctgcagggct tcccggatgg gagtgcggct catgcgcatt tccgccgcca
     2881 gggaattctc ggaaaaagag aaccggtcgg tggaccgtct ctggaggacc agtttccgga
     2941 tttcttcata cgcctggtct ttcttttttt tgcgcactcg gaacgctcct ctcgcggata
//
LOCUS       DAOTHD010000159         2993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_39036_length_2993_cov_3.022805, whole genome shotgun sequence.
ACCESSION   DAOTHD010000159 DAOTHD010000000
VERSION     DAOTHD010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39036_length_2993_cov_3.022805"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..1082)
                     /locus_tag="P5219_03765"
     CDS             complement(111..1082)
                     /locus_tag="P5219_03765"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRX25906.1"
                     /translation="MKLKKAVAAAAVCLAVLFAVPALAADFPARPVTYIIGWATGGGS
                     DLVSRVLCAEAEKHLGQPIVIVNKPGGSGAKAYVEISQAKPDGYTIGNTTGTISTHKF
                     MGVIPIGHEAFEVVITFNSDPGGLWVKKDAPWKTLKEFVEDARKRPGEITVAASNPGS
                     ITRLGLQLLELKENVKFKIASQSGGESAGPSLVAGGHVDACQAAPVTAKSLYDAGEIR
                     PLGIMSETRVEAFPDAPTCMEQGYDVVMHNARQILAPKGTPKEVVDKLYAAFKAAAES
                     ETFKKFMNESGSMILTWDQKQSTEFLEKQDAQFKELLQQVGLYKEAK"
     gene            complement(1102..2577)
                     /locus_tag="P5219_03770"
     CDS             complement(1102..2577)
                     /locus_tag="P5219_03770"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRX25907.1"
                     /translation="MTLLAAFQTVFTFKMIALIFGGSALGIIVGALPGLSATMGMALM
                     LPITFYMPPSEGILMLLGLYTGAVFGGAYSAILLNIPGAPPSVMTSLDGYPMAQRGEA
                     GRAISLATVGSTLGGIAGVIALIIIAPILARFALRFGPAEYTVLAVFGLSAIVAVSGK
                     SLARGFAGAALGVLLSTVGMDNFTNVSRFTFGSIELMSGINFIPVVIGLFGLAEVIAQ
                     VLETSKEGVITRTINRLFLSASDFGKVLKGLVPASLIGIVIGVLPGAGGTIASIISYN
                     HAMQSSKDPEKFGTGAPEGIIAAETANNASVGGAMVPLLTLGVPGSSPAAILLGALLI
                     HNLRPGPMLFTTQPVLINTIFVGLLAAQFSLLLWGLATARIAPWILAVRKEMMLPTIA
                     VLCVIGSFALKNSFFDVGIMLVSGVAGFFLNKLGVKPGTVILGLILGPMFENNLRSTI
                     EISGAAPIFTSPICIVLWVLTAAMVLYPICKMFRLRKCGTE"
     gene            complement(2620..>2993)
                     /locus_tag="P5219_03775"
     CDS             complement(2620..>2993)
                     /locus_tag="P5219_03775"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRX25908.1"
                     /translation="EEFRALSPKFFPDLLGWALLFLGGVIFVQGMRRPPAPVFRERPE
                     RGAVVRAILFILLVGLYLRFLPIIGFFSVTALFMLLSQALLGEKSIVMNLLRTGVFVF
                     LVNYLFSGLLHVPLPLGPWGF"
BASE COUNT          649 a          871 c          950 g          523 t
ORIGIN      
        1 tacgatgaat ttggattcaa cagtccgaca acaaaaaagg gctgcgcgaa cgcagccccc
       61 tggagggaaa cactgccgca ccgggcggca gaaacttcga aaaacgtccg ttatttcgct
      121 tccttgtaga gtcccacctg ctggagaagt tctttgaact gggcatcctg cttctcgaga
      181 aactccgtgg actgcttctg atcccaggtg agaatcatcg agccggactc gttcatgaac
      241 tttttgaagg tctcgctctc ggcagcggcc ttgaaagcgg cgtagagctt gtccaccact
      301 tctttgggcg tccccttggg ggcgagaatc tgccgggcgt tgtgcatgac cacgtcatat
      361 ccctgctcca tgcatgtagg ggcgtcgggg aacgcttcca cccgcgtttc gctcatgatg
      421 ccgagggggc ggatttcgcc cgcgtcatag agggatttcg cggtgacggg agccgcctgg
      481 caggcgtcaa cgtgaccgcc ggccacgagg gacggacctg cggactcgcc gcccgactgg
      541 ctggcgatct tgaacttcac gttctccttc agctcgagaa gctgcaggcc gagacgggtg
      601 atggagccgg ggttggacgc cgccacggtg atttcgccgg ggcgtttccg ggcatcttcc
      661 acaaactcct tcagggtctt ccagggagcg tccttcttca cccagaggcc gcccggatcg
      721 gagttgaagg tgatgaccac ttcgaaggcc tcgtggccga tgggaatgac gcccatgaac
      781 ttgtgggtcg aaatggtgcc ggtggtgttg ccgatggtgt acccgtcagg tttggcctgg
      841 gaaatctcca cgtaggcctt ggcacccgag ccgcccggct tgttgacgat gacgataggc
      901 tgcccaaggt gtttctccgc ctcggcgcag aggaccctgc tcaccaggtc ggagccgccg
      961 ccggtggccc acccgatgat gtaggtcacc ggacgggcgg gaaaatccgc cgcgagggcg
     1021 ggaaccgcga aaagaaccgc cagacacact gccgccgctg caactgcctt tttcaacttc
     1081 atgaactgca cctccccttt cttattccgt tccgcatttc ctgagcctga acatcttgca
     1141 gatcgggtac aggaccatcg ccgccgtcag cacccacagg acgatgcaga tgggcgatgt
     1201 aaaaatgggc gccgctccgg aaatttcgat ggtgctccgg aggttgttct cgaacatggg
     1261 ccccaggatc aggccgagaa tgaccgttcc gggctttacc ccgagcttgt tgaggaagaa
     1321 gcccgcaact cccgatacca gcatgatacc gacgtcgaag aagctgttct tgagcgcgaa
     1381 ggatccgata acgcagagaa cggctatggt cgggagcatc atttccttcc ggaccgcgag
     1441 aatccacgga gcgatccgtg ccgtggccag tccccagagc agcagggaaa actgggccgc
     1501 aagaagcccc acgaaaatcg tgttgatgag cacgggctgg gttgtgaaaa gcatgggtcc
     1561 cggccggaga ttgtggatga ggagggctcc cagcagaatt gccgcgggtg agctgcccgg
     1621 aacgcccagc gtcagcaggg gaaccatggc cccgccgacg gaggcgttgt ttgccgtctc
     1681 cgccgcgata atgccctcag gggcgccggt gccgaacttt tcggggtcct tcgaggactg
     1741 catggcgtgg ttgtagctga tgatggacgc tatggtccct cccgccccgg gaagaacgcc
     1801 gatgacgatg ccgatgaggc tcgccggaac aagtcccttc aggacttttc cgaagtcgct
     1861 cgccgagagg aagagcctgt ttatggtcct ggtgatgacc ccttccttcg aggtttcgag
     1921 gacctgggcg ataacctccg ccaggccgaa gagcccgatc accactggta tgaagttgat
     1981 tcccgacatc agttcaatgg agccgaaggt gaaccgggac acgttggtga agttgtccat
     2041 gcccaccgtg gacagaagaa cgccgagagc cgcgcctgca aacccccggg ccaaagactt
     2101 tcccgaaacc gccacaatgg cgctcagacc gaagacagcc aggacggtat attccgccgg
     2161 gccgaacctc agggcaaatc gtgccaggat gggagcgatg atgatcaggg cgatgacacc
     2221 ggcgatgccg ccgagggtgg atcccaccgt ggccaggctg atggcccgcc cggcttcacc
     2281 cctctgggcc atggggtacc cgtcgaggga ggtcatgacc gacggaggag cgccggggat
     2341 gttcagcagg atggccgagt aggctccgcc gaatacggcc ccggtgtaca ggccgagaag
     2401 catgaggatt ccctcggaag ggggcatgta gaaggtaatg ggaagcatga gggccatgcc
     2461 catggtggcc gagaggcccg gaagcgcccc cacgatgatc ccgagggcgc tgcccccgaa
     2521 gatgagggcg atcattttaa aagtgaatac cgtctgaaaa gctgccaaaa gagtcatgaa
     2581 cgtcatcctt tcactgacgg cgggcggaga aagccatcgt cagaatcccc acggccccag
     2641 gggaagggga acgtgaagca ggccggagaa gagataattc acgagaaaca cgaaaacgcc
     2701 cgtccgaagg agattcataa cgatgctctt ctcgccgagg agcgcctggg agaggagcat
     2761 gaagagggcg gtaacggaga agaagccgat gatcggcaaa aacctgagat agaggcctac
     2821 cagcaggatg aagaggatcg cccgcaccac tgccccccgc tcgggccgtt ccctgaagac
     2881 cggcgcggga gggcgccgca ttccctggac aaaaatcact cctccgagga aaaggagcgc
     2941 ccagcccagg agatcaggga aaaacttcgg ggaaagcgcc cgaaattcct cgg
//
LOCUS       DAOTHD010000160         2982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_39214_length_2982_cov_2.772463, whole genome shotgun sequence.
ACCESSION   DAOTHD010000160 DAOTHD010000000
VERSION     DAOTHD010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39214_length_2982_cov_2.772463"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..668
                     /gene="gspM"
                     /locus_tag="P5219_03780"
     CDS             117..668
                     /gene="gspM"
                     /locus_tag="P5219_03780"
                     /inference="COORDINATES: protein motif:HMM:NF041618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HRX25909.1"
                     /translation="MRPILARFFPSLSGKDDGGPGAERRALFLLLLGLLAWGGAFSLW
                     SDGRELRARRDLQKKRFDDLASVVGEYRALRQTAGTERIPVSPDGEEDLLTAVSNAVA
                     SLGLRSNMLSLSSTTGPGGSGAVSVTLEGLSSDSLARFLQETERRGIYSFSADLRAVR
                     STTSEGAPGRTLTAVLLLGRQGP"
     gene            665..1285
                     /gene="gspN"
                     /locus_tag="P5219_03785"
     CDS             665..1285
                     /gene="gspN"
                     /locus_tag="P5219_03785"
                     /inference="COORDINATES: protein motif:HMM:NF041619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HRX25910.1"
                     /translation="MKAGRWGLYLLFFLLVFSLSFLVFFPFGPGTEAAWSRAVRAASE
                     KGFFLDASSVSAQGRLLPGAVLLNVRLRSSLLSGEAGRVTVSLSPADMVTRLAPAAGL
                     RLERVSVNLPLPGERPLFLAAAEARLVFSDGRVEVTDLRISGELDVSGSAVLSFRGGG
                     LEEADLTIGGERAALMELFRSVLPLRQEKPGAWTLKRGRRSAHGGN"
     gene            1272..2177
                     /gene="gspC"
                     /locus_tag="P5219_03790"
     CDS             1272..2177
                     /gene="gspC"
                     /locus_tag="P5219_03790"
                     /inference="COORDINATES: protein motif:HMM:NF041616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HRX25911.1"
                     /translation="MAGIDGLLRGGARLVLPLLGTAFFAGMAGFWAAGLVEERLFLLS
                     VEETGKMAGRGAPTPLPAHKEQGPSLRDFAAGDPFGAAPPSPAAPSAKPAEMEVSVSE
                     LYELEGIRLAGTLPGIAVWIDVGGKQTVHLQGQQVKGYDLTEVGEDRVVLRKGGTVVN
                     VLLRYSGHASSGASAQPSPPPALSIPGRGVTAARPGQQGSIPRETVDNLLMNPLDEMK
                     KFRLRPKFDGDRALGVEVQWMDRNSFLESVGVEKGDVIQSVNGLEIRNMGDVVNVINS
                     LMGGSAFDVQVLRDGTPVSLKYNIK"
     gene            2186..2560
                     /locus_tag="P5219_03795"
     CDS             2186..2560
                     /locus_tag="P5219_03795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRX25912.1"
                     /translation="MIPGRRRAFTLAETLVAVAVLGLVAAGSMRLAVLSARALGEVRQ
                     GRERLALSRNFWLRAVGGKLESRGREKAVSWETGRFDFPGQDGPPPGYSCGTVVITPA
                     PAGGEKIVLYVPDRMISRGSKQ"
     gene            2557..>2982
                     /locus_tag="P5219_03800"
     CDS             2557..>2982
                     /locus_tag="P5219_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HRX25913.1"
                     /translation="MIRKLLLFALILFFVVPGALAQTAPQDDEFALVESARRMRASGN
                     AQFNFQDVDMVKFIRFMAELLNENLVLAPGVKGSISVISPKPFPLKEARQVFISVLEM
                     NGYTLQQGAGFGKVVPLQAGSSAESDVRTGKMGPGEGEQT"
BASE COUNT          489 a          891 c         1026 g          576 t
ORIGIN      
        1 atccaggctc ttcgttcggc ggccgattcc gggggcttca gggcttccct cggtgacatc
       61 cagcagattc ccggaggagg gctccggttt tccctctccc tgaggaggaa tgaccagtga
      121 ggcccattct tgcccgattt ttcccttccc tctccggaaa ggatgacggc ggccccggag
      181 ctgaacgccg ggcgctgttc ctgctgctcc tgggccttct cgcctggggg ggcgccttct
      241 ccctctggtc cgacggcagg gagcttcggg ccaggaggga tctccagaaa aaacggttcg
      301 acgacctcgc ctcggtggtg ggggaatacc gggcgctccg gcagacagcc ggaactgagc
      361 ggattcccgt ttcacccgac ggtgaagaag accttctcac cgccgtttcc aacgccgtgg
      421 cctccctcgg cctccggtcg aacatgctca gcctgagcag tacaacagga ccggggggct
      481 ccggcgccgt ctccgtgacg ctggaaggac tgtcttccga cagccttgcc cgcttcctcc
      541 aggagacgga gcggagaggc atctattcct tctccgccga cctccgggct gtcaggagca
      601 ctacgtccga aggggctccg gggcggaccc tcacggcggt gctccttctc gggaggcagg
      661 gaccatgaag gccggaaggt ggggattgta tctcctgttc ttccttcttg tattcagcct
      721 gtccttcctt gttttctttc ccttcgggcc cgggacggaa gcggcgtgga gcagggctgt
      781 ccgggcggcg tcggagaagg gctttttcct tgacgcgtct tccgtctccg cccagggacg
      841 tttgcttccc ggggccgtgc tcctcaatgt ccggctccgg tcatccctcc tgtcggggga
      901 ggccggccgt gtgacggttt ccctttcgcc ggcggacatg gtgacacgcc ttgccccggc
      961 ggccggactc cggctcgaac gggtctctgt gaaccttccc cttccggggg agcgccccct
     1021 ctttctcgcc gctgccgagg ccaggcttgt tttttccgac ggcagggtcg aagtgacgga
     1081 tcttcggatt tcgggggagc ttgacgtttc cgggagtgcg gtgctttcat tccggggagg
     1141 aggcctggag gaggccgatc tgacaatcgg aggggaacgg gcggcattga tggaactctt
     1201 ccggtccgtt cttccgctga ggcaggagaa accgggggcc tggaccctga agaggggaag
     1261 gaggagcgcc catggcggga attgacggcc ttctccgggg aggagcccgg cttgtccttc
     1321 cccttctcgg gacggctttt tttgccggaa tggcagggtt ctgggcggcc ggactggtgg
     1381 aggagcggct cttccttctc tccgtggagg agacggggaa aatggcgggc agaggcgcac
     1441 cgacacctct tcccgcgcac aaggagcagg gcccttccct cagggatttc gccgccgggg
     1501 atcccttcgg cgcggcgcct ccttctcctg cggcgccgtc ggcgaagccg gcggaaatgg
     1561 aagtttccgt gagcgagctc tacgaacttg aggggatccg gctcgcgggc acgttgccgg
     1621 ggatcgcggt ctggatcgac gtcggcggaa agcagaccgt ccatctccag ggacagcagg
     1681 tgaagggcta cgatctgacc gaggtgggcg aggacagggt agtcctcaga aagggcggaa
     1741 cggtggtgaa cgtcctcctc cggtacagcg gccacgcatc ctccggggcc tcggctcagc
     1801 cgtctcctcc tccggctctg agcatacccg gccggggagt cacggcggcc cggcccggac
     1861 agcagggttc cattcccagg gaaaccgtgg acaacctgct catgaatccc ctggacgaga
     1921 tgaaaaaatt ccggctcagg cccaaattcg acggcgacag ggccctgggc gtggaagtgc
     1981 agtggatgga caggaacagc ttcctcgagt ccgttggggt ggagaaaggc gacgtgatcc
     2041 agtcggtgaa cggactggag atccggaaca tgggggacgt ggtgaacgtc ataaactccc
     2101 tcatgggggg aagcgccttt gacgttcagg tgctcaggga cggcacgccc gtatccctga
     2161 agtacaatat aaagtgagaa gagccatgat cccgggaaga agaagggctt ttacccttgc
     2221 cgagacgctt gttgccgtgg cggtcctcgg gctcgtggcc gccggaagca tgaggctggc
     2281 ggttctttca gcccgggccc tcggggaggt ccggcagggg cgggagcggc tcgccctctc
     2341 caggaacttc tggcttcggg ctgtcggcgg aaagcttgag agccggggcc gggagaaggc
     2401 ggtttcatgg gagaccggca gattcgattt tccgggacag gacggcccgc ctcccggcta
     2461 ttcctgcggg acggtggtca tcactcccgc ccccgcgggg ggagaaaaaa tagtcctcta
     2521 tgttcctgac cgcatgattt cgagggggag taaacaatga tccggaaact tttgctgttc
     2581 gcacttatcc tgttcttcgt ggtgccgggg gccctggccc agacggcgcc ccaggacgac
     2641 gaattcgccc tcgtggagtc ggccaggaga atgagggcct cggggaacgc ccagttcaac
     2701 ttccaggatg ttgacatggt gaagttcatc cggttcatgg cggaactgct gaacgaaaac
     2761 ctcgtcctcg ctcccggtgt gaaggggtcc atttcggtca tttccccgaa gccctttccc
     2821 ctgaaggaag cccgccaggt gttcatctcc gtgctggaga tgaacggcta caccctgcag
     2881 cagggagcgg ggttcggcaa ggtggtgccc ctccaggcgg gaagctcggc tgagtcggat
     2941 gtgcggaccg gaaagatggg gccgggggag ggggagcaga cg
//
LOCUS       DAOTHD010000161         2975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_39307_length_2975_cov_3.082877, whole genome shotgun sequence.
ACCESSION   DAOTHD010000161 DAOTHD010000000
VERSION     DAOTHD010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39307_length_2975_cov_3.082877"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="P5219_03805"
     CDS             <1..390
                     /locus_tag="P5219_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRX25914.1"
                     /translation="LGQSSTTLSGGEAQRVKLSKELSKKFSGPTLYLLDEPTTGLYYT
                     DVEKLLVIINKIADQGNTVILIEHNLDVLMSADYIIDLGPEGGNGGGCLVAAGTPEDL
                     IHSCKGYTARFLREYSDQIGERMKKSG"
     gene            383..1219
                     /gene="rlmB"
                     /locus_tag="P5219_03810"
     CDS             383..1219
                     /gene="rlmB"
                     /locus_tag="P5219_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRX25915.1"
                     /translation="MDKMERKEGKRPSGRRFGKETGSSAPQFEKKRSEDLNWGRQVVL
                     QLLKESPDRVQKIFLGKNVSESFSRQIKTLAEKGGVVVQVLAPEVLSEICGPVNHQGV
                     ACKSSEVSMADLGDFLSSLPSGGPLLAVILDHVQDPHNLGAIARTAEACGAKGVLFPK
                     RRGALPGGTVVKVSAGAALRLPMVGVNNVVNTIKELQEAGFWTIGLDNRTGRSLWSEP
                     MPEKTALIVGAEGEGLSRLVSDTCDEIVRIPLMGETGSLNASVACAVGMFEWARKWGP
                     QS"
     gene            complement(1319..2242)
                     /locus_tag="P5219_03815"
     CDS             complement(1319..2242)
                     /locus_tag="P5219_03815"
                     /inference="COORDINATES: protein motif:HMM:NF014476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HRX25916.1"
                     /translation="MDAAEEAMKAAAREKEGAITVPLRTAVSREKNSLLLMPCLADDF
                     WGLKALTIFPDNPVRSRPFLNGLVLLFDGKDGTPLALFDGRTLTALRTGSVGGAAVRA
                     LAGTDVRSLGLAGAGVQGYWQVRFACEARSFSEVRIFDVFPENAERTAERLGKDLPGI
                     SVFPCRNAEELALSSDVVITATTAKSPVFPDDRRLFSGGKCIIGIGSYTPEMREYPDA
                     LFAEAGQVYVDTEHALSETGDLINPLSRGVFSKEIIRPLSDVLLAGNKETARPGTVFF
                     KSVGLSVFDLYAAKTLLERGKMTGNGIEFEL"
     gene            2715..>2975
                     /locus_tag="P5219_03820"
     CDS             2715..>2975
                     /locus_tag="P5219_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRX25917.1"
                     /translation="MDLIEEHPILDFPRGEKVSFFFDGREMEGYEGEPIAVALHANGI
                     RVYRETAEMHRPRGFFCAIGKCSSCFMVVDGVPNVRTCITPLK"
BASE COUNT          757 a          793 c          794 g          631 t
ORIGIN      
        1 cttggccagt ccagcaccac tctttccggc ggcgaggccc agagagtgaa gctgtccaag
       61 gaactgagca agaaattctc tgggcccacc ctgtaccttc ttgacgaacc gactaccggc
      121 ctttactaca cggacgtgga aaagcttctg gtcatcatca acaaaatcgc cgaccaggga
      181 aacacggtca tcctgataga acacaacctg gatgtcctca tgtcagcgga ttatattatc
      241 gacctcggcc cggagggagg aaacggagga gggtgtctcg tcgccgcagg cacgccggaa
      301 gacctcattc actcttgcaa aggttatacg gcccggtttc tcagagagta ttccgatcaa
      361 atcggtgaaa ggatgaaaaa aagtggataa aatggaaaga aaagagggaa aaaggccatc
      421 aggcaggagg ttcggaaaag aaaccggttc ctccgccccc caattcgaga agaaaagaag
      481 tgaagacctc aactggggga ggcaggtcgt gctccagctc ttgaaagagt ccccggacag
      541 ggttcagaaa atattcctcg ggaaaaacgt gagcgaatct ttttcccggc agataaaaac
      601 cctggcggaa aaaggcgggg tagtggtcca ggttttggca cccgaggttc tttcggagat
      661 ttgcgggccg gtaaaccacc agggagtcgc atgcaaaagc agcgaggttt ccatggcgga
      721 cctcggggat tttctctcct cgctcccttc gggcgggcct ctccttgcgg tcatcctcga
      781 tcacgtccag gacccccaca accttggagc catagcccga acggctgaag cgtgcggcgc
      841 gaaaggagtt ctctttccca aacgcagggg ggccctcccc gggggtacag tggtcaaggt
      901 gagcgccggg gcggcattgc gtcttcccat ggtgggtgta aacaatgttg ttaatacaat
      961 caaagaactc caggaagccg gcttctggac catcggactc gataacagaa cgggccgttc
     1021 tctctggtct gagcccatgc cggaaaaaac cgccctcatc gtgggggctg aaggtgaagg
     1081 tttatcaagg cttgtctccg atacctgcga tgaaattgtc cgaatccccc ttatgggaga
     1141 aaccggctct ttgaatgcaa gcgttgcgtg tgccgtggga atgttcgaat gggccaggaa
     1201 gtggggtccc cagtcctgaa aaaatcaccc ctgcatcacc tgttcagttg atttcatcga
     1261 accggggaac ggctccagtc aaacgggagc cgttccccgg ctgttcggca taaaaaagct
     1321 acagctcgaa ttctataccg tttcctgtca tttttcccct ctccagcaaa gtcttcgcgg
     1381 cataaagatc aaacaccgaa agtccgacgg atttgaagaa aacagttccg gggcgggccg
     1441 tttccttatt tcccgctaga agaacatccg aaaggggacg gatgatctct ttcgaaaaaa
     1501 ctcccctgga aagcgggttg atgagatctc ccgtttctga aagagcgtgc tccgtatcca
     1561 cgtaaacctg tcctgcttca gcgaaaaggg catcggggta ctccctcatc tcgggagtat
     1621 aggaaccgat gccgatgatg cacttccctc ccgaaaataa acgcctgtca tcaggaaaaa
     1681 caggcgattt cgctgttgtt gcggtaatga ccacatcgga agaaagagcc agctcctcag
     1741 cattcctgca cgggaacacg ctgatgcccg gcaggtcttt tccgagcctc tcggcggttc
     1801 tttcagcatt ctccgggaag acatcaaaga tccggacctc tgaaaaggat cgggcctcgc
     1861 aggcgaaacg gacctgccag tatccctgga cacccgctcc ggcaagacca agggaccgca
     1921 catctgttcc cgcgagggcg cgcacagccg cccctcccac cgaccccgtt ctcaaggcgg
     1981 tcagtgttct tccgtcaaaa agcgcaaggg gagttccgtc cttcccgtcg aaaagaagta
     2041 cgagcccgtt cagaaaaggc cgtgagcgta cagggttgtc gggaaaaatc gtgagagcct
     2101 tcaggcccca gaagtcatcg gcaagacagg gcatgagaag gagagaattc ttttccctgc
     2161 tgaccgcagt cctcagggga accgttatgg ctccttcctt ctcccgagcg gccgctttca
     2221 tcgcttcttc tgcggcatcc atcaacccaa ggaaagtgaa acatgatagc atgtggctgc
     2281 tgttcatgag aaacacaccg gatcacctct ttcttgaaaa taatcgtact ctcgtgaaaa
     2341 acaggttcta ttctatttca aattttcctc ccgagcaaaa acgatccgac ttttctcaga
     2401 ggaaagatcc gttttcgaaa gtgcgtaacg aaccatttgt gaaaaagttg aaagtgccca
     2461 aaatttcaac atatccttga caaattcgat ttcccggaac acaattgaac acacaaaagc
     2521 attctccgtc acccattttc tacggagggg atagtgctgc ccatcatttc atgtccgcaa
     2581 aaaacagccc tttgtgctcc tgattccagt attttttccg caacaggcca tgcgtgttgt
     2641 gaaatggggt catgcttgga aggaggaaaa caggcttgga tgagaaaagt aatccgggag
     2701 tagaccgcgg gacaatggat ttgatcgagg aacatccgat ccttgatttc ccgagggggg
     2761 aaaaggtcag ctttttcttc gacggcaggg aaatggaagg atatgaaggg gagcctattg
     2821 ccgtggccct tcatgcgaac ggaataaggg tctaccgtga aacagcggaa atgcacaggc
     2881 cccggggatt tttctgcgcc atcggtaaat gcagcagctg cttcatggtg gtggacgggg
     2941 ttcccaacgt ccggacgtgc atcacccccc tgaag
//
LOCUS       DAOTHD010000162         2975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_39312_length_2975_cov_2.459247, whole genome shotgun sequence.
ACCESSION   DAOTHD010000162 DAOTHD010000000
VERSION     DAOTHD010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39312_length_2975_cov_2.459247"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="P5219_03825"
     CDS             complement(<1..295)
                     /locus_tag="P5219_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRX25918.1"
                     /translation="MQEVKKVITEVGTNEWQVVVFFLGDQTFAINVDKTREILRWPGC
                     RTIPESHPSMIGITSVRGEVLPLLDLRKHLAIPGNTDLEQSKVIIAEFNEIKLG"
     gene            425..1810
                     /locus_tag="P5219_03830"
     CDS             425..1810
                     /locus_tag="P5219_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRX25919.1"
                     /translation="MADNTEALRSGPMGALLFRLSLPAALGMAVQASYSLVDAFFVGQ
                     GVGSAGIAAVSMTFPLQMIIMAVAQAGGVGGASVISRSLGAGRRKRAEKTAGAVFFVT
                     WASGILLSFLWILLAPFLLRLMGTSGDILPLAEDYAAVLFLGAPLFAFSITANNFVRA
                     EGNASFAMMTMIISAGINTLLDPLFILGFGWGVRGAAWATVLSQGGTALWLGWYYLEG
                     HSLVAFRWKHFRFRASILAECFSLGAAAFARQGAASLSLLAVNLALASTGGDDAVAAF
                     GIVNRVLLFAIMPVFGVVQGLLPVVGFNYGAKQYCRVVSALRISIGASTVLCTAGAAL
                     FLTVPEAITGLFTTSAAVTALGADAARMLALGMPLTGFQIMASGLFQAIGKTRPSFVL
                     SLLRQVILLVPLVTVLAPVFGTAGVWAAFPAADLSAALLTFLLYRNEMRRLRNSCGSG
                     AEDSPAGTAGS"
     gene            complement(1807..>2975)
                     /locus_tag="P5219_03835"
     CDS             complement(1807..>2975)
                     /locus_tag="P5219_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25920.1"
                     /translation="HYWKRFGEGTAFSMAKNTLSRIWAGGIHDHLGGGIARYATDRRW
                     VLPHFEKMLYDQALLLHTVAEFHRESPDPLFKAFAEDLAGFVLREMTSPEGGFYSAFD
                     ADSEGEEGKYYLWTEEQIRSILSPEEAGVFLHSYGVLRGGNFKNEVTGRILGDNVLYM
                     AVSPGDAAARFALEPEEIGGLLFSCREKLLAERRKRPAPLLDDKILTDWNGLMIAGLS
                     LAGTVFGRKEWVTAAEKAARFIELKLREKTGRLLHRYRDRDAAIPGFLDDYAFLAWGL
                     TELGAATGKKDYVLSAAKLMDILLEDFSDGKKGGFFPHGGQDGNLFLRRKEAYDGAIP
                     SGNAVAMANFLRLAGPAGRKDFPDHARRTGGAFSEHVSRYPLSHAHLLTAVLMF"
BASE COUNT          486 a          958 c          898 g          633 t
ORIGIN      
        1 aaccgagctt gatctcgttg aattcggcta tgatcacctt gctctgctcg agatccgtat
       61 tgccgggaat agccaggtgc tttctcaggt caagaagggg gagcacctct cctctcacgg
      121 aagttattcc gatcatggac ggatgggatt ccgggatggt tcggcagccg ggccaccgga
      181 ggatttcacg ggttttgtcc acgttgatgg cgaaagtctg gtctccgagg aaaaagacca
      241 ccacctgcca ttcgttggtc cccacttcag ttataacttt tttgacttcc tgcataggat
      301 ctccctcctc cggtcttccg gatgatgaag ctgaagctac tatagccgaa ggactgccat
      361 accacaagcc ccgcccatcc ctttctgata tacttctgaa aaggaaaggg aagggagatg
      421 cgacatggcc gacaacaccg aagcgctgcg ctccggcccc atgggggccc tgctcttccg
      481 gctgtccctc ccggcggctc tcggcatggc cgtccaggca tcctacagcc tcgtggacgc
      541 ctttttcgtc ggtcagggag tgggaagtgc cggaatcgcc gcagtctcca tgaccttccc
      601 cctccagatg atcatcatgg cagtggccca ggcagggggt gtcggagggg cctccgtcat
      661 ctccagaagc ctcggcgcag ggcggcggaa gcgggcggag aagaccgccg gagccgtctt
      721 cttcgtgacc tgggcatccg ggatcctgct ctccttcctt tggatcctcc tggctccctt
      781 tctcctccgg ctcatgggaa ccagcgggga catccttccc ctggcggagg attacgccgc
      841 cgtgctcttc ctcggagccc ccctcttcgc tttttccatc accgccaaca acttcgtccg
      901 ggccgagggg aacgcttcct tcgccatgat gaccatgatc atttccgcgg gaatcaacac
      961 gctcctcgat cccctgttca tcctcggctt cgggtggggg gtgcggggcg ccgcctgggc
     1021 caccgtactc tcccaggggg gaaccgctct ctggctggga tggtattacc ttgaaggaca
     1081 cagtcttgtg gctttccgct ggaaacattt ccggttccgg gcctccatcc tcgccgaatg
     1141 tttttcgttg ggggcggcag ctttcgcccg gcagggggcg gccagtctct ccctgctggc
     1201 ggtgaacctg gccctggcct ccacgggagg agacgacgcc gtggcggcct tcggcatcgt
     1261 gaacagggtc cttctcttcg ccatcatgcc agtgttcggc gtggtccagg gactcctccc
     1321 cgtggtgggg ttcaactacg gggcaaaaca gtactgccgg gtggtgtctg ccctccggat
     1381 ctccattgga gcctcaacgg tcctctgcac cgccggcgca gcgctctttc tcaccgttcc
     1441 tgaggccatc acggggctgt tcaccacctc cgccgcagtc acggctctcg gtgcggacgc
     1501 ggcccggatg ctcgccctcg gaatgcccct cacgggattc cagatcatgg cgtcgggtct
     1561 tttccaggcc atcggcaaga cccgtccctc cttcgtcctc tcactgctgc gccaggtcat
     1621 cctgctcgtc cccctggtga cggtcctcgc ccccgtcttc ggcacggcag gagtgtgggc
     1681 ggcatttcct gccgcagatc tgtctgccgc cctgcttacc tttcttttgt acaggaatga
     1741 aatgcgccgc ctccggaaca gctgcggaag cggcgctgaa gattctcctg cgggaacggc
     1801 cgggagctag aacatcagga ctgccgtgag aaggtgggcg tgggagaggg ggtacctgga
     1861 gacgtgctcc gagaaggccc ccccggtgcg ccgggcgtgg tccgggaagt cctttctccc
     1921 tgccggtccg gcgaggcgga ggaagttggc catggccacg gcgttcccgg agggtatggc
     1981 tccgtcatag gcctccttcc ttcggagaaa aagatttccg tcctgtcctc cgtgagggaa
     2041 gaagcccccc ttcttcccgt cggaaaaatc ctccaggagg atgtccatga gtttcgccgc
     2101 ggaaagaacg tagtcttttt ttcccgtggc ggctccgagt tccgtcagcc cccaggcgag
     2161 gaaggcgtaa tcgtcgagaa atccggggat ggcggcgtcc ctgtcccggt acctgtggag
     2221 gagcctgccg gttttctccc gaagcttgag ttcgatgaac cgggccgcct tttccgccgc
     2281 cgtcacccac tccttccggc cgaacacggt ccccgcaagg gagagtccgg cgatcatgag
     2341 gccgttccag tcggtgagga tcttgtcgtc cagcaggggc gccggacgct tgcggcgttc
     2401 ggcgagcagc ttttccctgc aggagaagag cagtccgccg atttcttccg gctccagggc
     2461 gaaccgcgcc gccgcgtccc cgggggaaac ggccatgtag agcacgttgt ctcccaggat
     2521 gcgccccgtg acctcgtttt tgaagttgcc tccccggagg actccgtagg agtggaggaa
     2581 cacccctgcc tcttccggag aaaggatgct ccggatctgc tcctccgtcc agagatagta
     2641 ctttccttcc tccccctcgc tgtcggcgtc aaaggcggaa tagaagcctc cctccggtga
     2701 ggtcatctcc cggagaacaa acccggcaag gtcttcggcg aatgctttga acagcgggtc
     2761 cgggctctcc ctgtggaatt ccgccacggt atggagaagc agcgcctggt cgtagagcat
     2821 tttctcgaag tggggcagaa cccaccggcg gtcagtggcg taccgggcaa tgcctccgcc
     2881 aagatggtcg tgtatgcctc cggcccagat gcgggacagg gtattctttg ccatggaaaa
     2941 ggccgtccct tctccgaagc gcttccaata gtgaa
//
LOCUS       DAOTHD010000163         2971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_39374_length_2971_cov_3.350823, whole genome shotgun sequence.
ACCESSION   DAOTHD010000163 DAOTHD010000000
VERSION     DAOTHD010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39374_length_2971_cov_3.350823"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="P5219_03840"
     CDS             complement(<1..406)
                     /locus_tag="P5219_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutP family protein"
                     /protein_id="HRX25921.1"
                     /translation="MIWKKIFNTPQEELEDEPVEMEAGAGAQCPEDDIPRASHQHHGR
                     EREPLSLDINSRGGERDQPGIELNLKIRIDDESQVGRAALLLAATTSNETECVFKKEL
                     SSIGWRSVATEVGGLAGDLPQKITRALVGASLN"
     gene            661..1623
                     /locus_tag="P5219_03845"
     CDS             661..1623
                     /locus_tag="P5219_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRX25922.1"
                     /translation="MKKLLFAVFTAFVFFAVPVGAASARVFLSIATGSTGGTYYPLGG
                     GIAEIISKNVPEFQVTSETGNASAANLNLIGTRQIEMAFAQNDIAYWASKGMPPFKER
                     YENIRVVASLYPEHVHGITLKGSGVNDITDIRGKRVSVGAPGSGVLGDVSAILKIAEL
                     HYGDMSPDFLDFNNTTQRFKDGQLDVGFVVAGYPTSSIIDLAATNDIDLISFNDDFMT
                     KLTEEYPYFIKDVIPAGTYRGIDSDIVTPAVMAMLVCEASLSDDVVYRFTKALWENIA
                     DLHKVHPKAGLITLVTALDGVSVPVHPGAARFYTERGMTVPKVK"
     gene            complement(1657..2853)
                     /locus_tag="P5219_03850"
     CDS             complement(1657..2853)
                     /locus_tag="P5219_03850"
                     /inference="COORDINATES: protein motif:HMM:NF019585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25923.1"
                     /translation="MTVSFRVTILDVLASDEKKKKFTLLLSGGELHPSGGGQPGDSGV
                     LWAENFRFRIDDTEKRDGGVAVSGKAEKGSPAAGMEVEGEADLERHRLLSRMHTGEHI
                     LSRALEKAHPGLRVFKVAVNSAETSVYLTWDGDLGWDMLFEAEKEGNRIVSQNLPVET
                     VMLSKKEAEHLPGIKGNWDRIADETIRVVRIPGYDTIACSGSHASSTGEVGNLFITAF
                     RGTAPDWEFKFSVDGVALRQEYSEAARRLVRGIGCRLPQLGQVVVGLQEENGALKKLL
                     ERASQYVSLPAEDSSAGWLPVSAAVLPGFSRELAAPAARKWSDANPGRVLLLLMPEPE
                     KKEGSFLIYGGKEVPVDFSAFLKESPSLRARGGGRNDWLNGLSPVMEAEPWLDALKTF
                     AEKRRP"
BASE COUNT          629 a          893 c          765 g          684 t
ORIGIN      
        1 cgttcagtga tgcccccaca agggccctgg taattttctg gggaagatct cccgcaagcc
       61 ctcccacctc cgtggcgacc gatctccagc caatggatga cagctctttc ttgaaaacgc
      121 actccgtctc gttgctggtc gtcgcggcga gaagaagggc cgcccttccc acctggcttt
      181 cgtcgtcgat ccgaatcttg aggttcagct caattcccgg ctgatccctc tcccctcccc
      241 tggaattgat atccagcgac agcggctccc tttcccggcc gtgatgttga tgagaagccc
      301 tgggtatatc gtcttcagga cattgtgctc ctgctcccgc ttccatttcc acaggttcgt
      361 cctccaactc ttcctggggg gtgttgaaaa ttttcttcca gatcacgaac atgcagctcc
      421 tctctggtga attctagtac gcattataaa ggaacaggag aaaagagtca cttcccggac
      481 cgtttttcct gcaatggagg atccagggta aggaaaattg ctgaaaaggg agatagattc
      541 cttgtccccc ggcaccttcc ggtttatact tgtctgggga aaaactctgg ggatgtcgtt
      601 tcctatggct gttccggaca tgaacctcag atctttcatt tacgcaaggg ggcgttttag
      661 atgaagaaac tgcttttcgc ggtcttcact gcttttgttt tttttgctgt tcctgtcggg
      721 gcagcttccg ccagggtctt cctttcaatc gccaccggga gcacgggggg aacctattat
      781 cccctcggcg gagggatcgc ggaaatcatc agcaaaaatg tccctgaatt tcaggttaca
      841 tccgaaacag gcaatgcttc cgctgcaaac ctcaacctca tcgggacccg ccagatcgaa
      901 atggctttcg cccaaaacga tatcgcctac tgggcctcga agggaatgcc ccccttcaag
      961 gagagatacg agaacatacg cgtcgtggcg tctctttacc cggagcatgt tcacggaatc
     1021 accctgaagg gaagcggcgt caatgacatc acggacataa ggggcaagag agtgtccgtc
     1081 ggcgcccccg gatcgggtgt cctgggggat gtatccgcta tcctgaagat agccgaactt
     1141 cactacgggg acatgagtcc cgactttctt gacttcaaca acaccaccca gcggttcaag
     1201 gacgggcagc tcgacgtagg cttcgtcgtc gcaggatatc ccacgtcctc catcatcgac
     1261 cttgcagcca cgaacgatat tgatcttatc agcttcaatg acgatttcat gacaaagctc
     1321 accgaggaat atccctactt catcaaggat gtcatacccg ccggaacata caggggaata
     1381 gatagcgaca ttgtgacgcc tgcagtgatg gcaatgcttg tctgtgaggc aagcctttcc
     1441 gacgatgtgg tctacaggtt cacgaaggcc ttgtgggaaa acatcgcaga ccttcacaag
     1501 gttcacccaa aagcggggct catcaccctt gtcacggctc tcgacggtgt ttccgtgccc
     1561 gtccaccccg gagcggctag attttacact gaaagaggaa tgaccgttcc caaggtaaaa
     1621 tagctcgttc ccgaaacccg cagaaaaggc ggcccgtcag ggccgccttt tctctgcaaa
     1681 ggttttcagc gcatccagcc agggctcagc ctccatcacc ggagaaaggc cgttgagcca
     1741 gtcgttcctc cccccgcccc tggctctgag cgagggactt tccttcagga aggccgaaaa
     1801 atcgacgggt acctcttttc caccatagat gaggaaagag ccctcttttt tttccggctc
     1861 cggcatcagc agcagcaaca ccctcccggg attggcatcg gaccatttcc gtgcagcggg
     1921 ggctgcgagt tcccttgaaa aaccgggaag gactgcggcg gagaccggga gccatccggc
     1981 cgaagaatcc tccgcaggaa gggagacata ttgagaggct ctctccagca gcttcttcag
     2041 agctccgttt tcttcctgga gcccgacgac gacctggccg agctggggaa gcctgcagcc
     2101 tattcccctg acaagcctgc gggccgcttc gctgtactcc tggcggagtg ccaccccgtc
     2161 cactgaaaac ttgaattccc agtccggcgc ggttccccgg aaagccgtta tgaagaggtt
     2221 tcctacctca ccggtggacg aagcatggct ccccgaacag gcgatggtgt cataccctgg
     2281 aatgcggacc accctgatgg tttcgtcggc gatcctgtcc cagttgcctt tgattccggg
     2341 caggtgctcc gcctctttct tggaaagcat gacggtttca acgggaagat tttgcgaaac
     2401 gatccggttg ccttcctttt ccgcctcgaa gagcatatcc cagcccaagt ccccgtccca
     2461 ggtcaggtat accgaggttt cggcgctatt cacggcaacc ttgaaaaccc ggagccccgg
     2521 atgtgctttc tcgagagccc ttgaaaggat atgctcccct gtgtgcattc tcgagaggag
     2581 acgatggcgt tcaaggtccg cttctccttc cacttccatt cctgcggcag gggaaccttt
     2641 ttccgccttt cccgagacgg ccactcctcc atcccttttt tccgtgtcgt cgatgcggaa
     2701 ccggaaattc tccgcccaga gaaccccaga gtctccgggc tgccctccgc cggaaggatg
     2761 aagctctccg ccggagagaa gaagggtgaa tttcttcttt ttttcgtccg atgcaagaac
     2821 atccagaatg gtcaccctga agctcactgt caccgcccct ttcctgccga tgtaaaaata
     2881 gcacaaagcc gggattaatc ccggctttcg atttttctat ggcgcgcctg tggcgattcg
     2941 aacgccagac cctcggctcc ggaggccgat a
//
LOCUS       DAOTHD010000164         2960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_39562_length_2960_cov_3.062651, whole genome shotgun sequence.
ACCESSION   DAOTHD010000164 DAOTHD010000000
VERSION     DAOTHD010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39562_length_2960_cov_3.062651"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      336..436
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            510..1714
                     /gene="metK"
                     /locus_tag="P5219_03855"
                     /pseudo
     CDS             510..1714
                     /gene="metK"
                     /locus_tag="P5219_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047560.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
     gene            1826..2257
                     /locus_tag="P5219_03860"
     CDS             1826..2257
                     /locus_tag="P5219_03860"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX25924.1"
                     /translation="METRGTLLEIRFSTIEQFAPYRERILSVFRNLGEREGMLFFVAL
                     NEAVNNALIHGGTGGEGRAVELSVRDEGPRICAAIRNCGGCFSHDMKPDPEVFDNVFR
                     ESGRGVQIILHIADECSIGESGRLVSLCMNKPGAAAGGQGE"
     gene            2265..>2960
                     /locus_tag="P5219_03865"
     CDS             2265..>2960
                     /locus_tag="P5219_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25925.1"
                     /translation="MKECGGAPLSMEMAGFRFDGMNLLDMFCEALVIWEASTGAVLYT
                     NESARALYGYSCGEASALSIDDIFPDWEGGLRKGSFSIRTATHRKKDGMFFPVQLTCR
                     SLPGNAGDVMMMVASDVSPENLIHDSVSLAASIQRGFLPGDLEGDGRIEVRGIYRPMH
                     LISGDMFGYQWDERQGILSGYLFDVMGHGVPAALRTSAIMVLFHQAFEDDARAGASLE
                     EKLAWVNSMAEGGI"
BASE COUNT          673 a          782 c          905 g          600 t
ORIGIN      
        1 agaaagtact ctgctcggtg tatctccgct caggatagcg gggatcgaga taggctcttc
       61 gcgacctgtc ggttccgtca atgggcagga cagaaaaggc tgaaaccatg ccatccacct
      121 ccgtcatcct attccggtta aaaatatact gcccggataa gaatatgtca aacgattccg
      181 ttttctcgtt ttttcccctg ttttaaaaaa aacggtcccc ttccgggaaa gggggagagg
      241 ggaatcctcc gccagaaaaa ttgattattg acaggagtgg aggatagcgt ataatccttc
      301 tgttagtttt gtgtcttcaa ctttgacaac tgagactctt atcaagagag gtggagggac
      361 tggcccgatg aagcccggca acctgcctga aaaggtatgg tgccaatacc agcagccgca
      421 ggctgagtga taagagagtg cgtttgatga cccttcaaag gccctcttgt gagggtcttt
      481 ttttattttc acacaaggag gaaaaacaat tgacaaatga gcggtacctc ttctcttccg
      541 aatcagtgac agaaggtcac cccgacaagg tggcggacca gatttcagac ggtgtcctcg
      601 acgccatcct gaaggacgac cccatgggcc gggtggcctg cgaagtgttg tgcaccaccg
      661 gaatggtcat ggtggcgggg gagataacga cccagaccta cgtggacatc ccgaagattg
      721 ccagggatat cgtgaaggag ataggctaca ccagggcgaa atacggtttt gacggtgata
      781 cctgcgccgt gatcaccgcc attgacgagc agtccgaaga tatcaggagg ggggtggaca
      841 cggctctcga gatccgggaa catctcgtgt ccgaggaaga gatcgccagg atcggcgcgg
      901 gagaccaggg aatgatgacc gggtttgcct gcaatgaaac ccaggagtat cttcccatgc
      961 ccatagccct tgcccaccgc ctcgcccgca gactggccga ggtcaggaag aacgggacga
     1021 tcccctatct ccgcccgacg gaaaaaccca ggtgaccctg gagtacgttg acggcaaagc
     1081 tgttcacgcg gataccatcg tcgtctccgc ccagcaccat ccggaagcga cgaacgagga
     1141 gatccgggcg gatatcgtgg agcacgtgat cagccctatc gttccgaaag aactgatgaa
     1201 cggcaggacc aggatcctgg tgaacccctc ggggcgcttc gaaaagggag gccccatggc
     1261 ggacacgggg ctcaccggca ggaaaatcat tgtcgatacc tacggaagct gggtcgccca
     1321 cgggggcggc gctttctccg gcaaggaccc caccaaggtg gaccgttcgg gtgcctacat
     1381 ggcccgctac gtggccaaga acgtggttgc tgcgggactg gccgaccagt gccttgtaca
     1441 ggtggcttac gccataggtg tggcggagcc ggtttccgtc atggtggaca cctggggaac
     1501 cggcaagggg attaccgatg aagaaatcac gaccctcgtc aggaaacatt tcgattttcg
     1561 ccccgcagcc atcatccgtg acctcgaact gagaaaaccc cagtacagaa ggctggcagc
     1621 ctatggccac atgggccgga tcgacctgga tccccttccc gcgtgggaaa ggacggacaa
     1681 ggcggagatt ctttccgctg cgaaaaagaa gtaacggaaa acatgagcgt aaggggccct
     1741 gaggggcccc tttttatttg aaaagaaccg tgtggtatga ttgaaaaatc cggtacacaa
     1801 tggtgccccg aagggagcga tgcggatgga aacgagggga accctgctgg aaatccgctt
     1861 cagcactatt gaacagtttg ccccgtacag ggagcggatc ctttccgttt ttcgaaatct
     1921 cggcgaacgg gagggcatgc ttttcttcgt cgcactcaac gaagcggtga acaatgccct
     1981 gatccatgga ggcacggggg gtgagggacg ggctgtggag ctttccgtcc gggatgaagg
     2041 tcccaggatc tgtgccgcca tccgaaactg cggcggctgt ttttcccacg acatgaagcc
     2101 tgacccggag gtttttgaca atgtctttcg ggaatccgga aggggagtgc agattattct
     2161 tcacatcgcc gacgaatgca gcatcgggga atccggccgg ctggtcagcc tgtgcatgaa
     2221 caagcccggg gcggcagccg gcggtcaggg agagtgaacc ggtcatgaaa gagtgcggag
     2281 gagctccgct gtccatggag atggccggtt tcaggtttga cggtatgaac ctccttgata
     2341 tgttctgtga ggctcttgtc atttgggaag cttccacggg agcggtcctg tatactaatg
     2401 aatcggcgag agccctgtac ggttattcct gcggagaggc ttccgccctg tccatagacg
     2461 acatttttcc tgactgggaa ggggggctcc ggaaaggttc cttttcgatc cggacggcga
     2521 cccacaggaa gaaggacggc atgttctttc cggtccagct cacctgcagg tccctcccgg
     2581 gaaatgcagg ggatgtgatg atgatggttg cctcggatgt ctcgccggaa aacctcatcc
     2641 acgacagcgt ctccctcgcg gcatcgatcc agaggggatt tctgcccggg gaccttgaag
     2701 gtgacggacg gatcgaggtc aggggcattt accgtcccat gcatctcatc agcggcgata
     2761 tgttcggcta tcaatgggac gaacggcagg gaatcctcag cgggtatctc ttcgacgtca
     2821 tggggcacgg cgttcccgcc gccctccgga catcggccat catggtcctc ttccaccagg
     2881 ccttcgagga tgacgcccga gcgggggctt ctcttgaaga aaaactggcc tgggtgaact
     2941 ccatggcgga gggtggcatt
//
LOCUS       DAOTHD010000165         2959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_39579_length_2959_cov_3.175620, whole genome shotgun sequence.
ACCESSION   DAOTHD010000165 DAOTHD010000000
VERSION     DAOTHD010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39579_length_2959_cov_3.175620"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="P5219_03870"
     CDS             complement(<1..525)
                     /locus_tag="P5219_03870"
                     /inference="COORDINATES: protein motif:HMM:NF022743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HRX25926.1"
                     /translation="MESSIKGRLLRYWKVYAGALVLAVLSDMIGIVKFDIGIGTLTLF
                     PMVFATIIGGLFGPDLLRLFDMKESGAAGAMVLVCLAPFMAKMGVGAGANLARLVEVG
                     PALLLQEFGNLGTILISLPVALLLGLKKEAIGACYSINRDSNLGLTTDIYGPDAPETK
                     GTFAVYIVGSVIGTV"
     gene            complement(903..1436)
                     /locus_tag="P5219_03875"
     CDS             complement(903..1436)
                     /locus_tag="P5219_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25927.1"
                     /translation="MKKTLFLFFAAAILLFCVSPSDAEPFTEKMRHLLKDLPGWEGME
                     MVPIVTPDSGQGRAAVFTREYHQGRNLVVQALKMPGNPDGRDDFEETALDVSSERMRQ
                     GVIDGIRYAEIVYRVSPERKVPEATLYVFLPPYSLSRSAPETIVFTLNSINGDDELFH
                     LARTFDWKAILGALGEL"
     gene            1634..2425
                     /locus_tag="P5219_03880"
     CDS             1634..2425
                     /locus_tag="P5219_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HRX25928.1"
                     /translation="MFFIKDREKALRRYDFFLIFLAVASVGLIIAEERMDFTEAEEGI
                     FGLVDTAVWLIFVGDYFSRLFLAGDKKLYVKTHIIELIAILPFDALFKGLRSLRLFRM
                     LRSTRALRILRLIRLAAYFGRAHRYASRFLRQHNFQYVLFFTVLTVLIGAAAVRYFEQ
                     MGFYDALWWAFVTATTVGYGDISPSTAGGRIVAIMLMLVGIGFISTLTGTIASFFVAP
                     APEPEAKPKNEFLSLALQRLENFENLSTEEVREICAVLQGLKEKQ"
     gene            complement(2443..>2959)
                     /locus_tag="P5219_03885"
     CDS             complement(2443..>2959)
                     /locus_tag="P5219_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25929.1"
                     /translation="LDVGEDVLGKLEYAIASLHPPCIPFGTMEQNTRALIGAMANPYV
                     KIIGHPDDDRYPLDYEEVVAAAQREKVALEINNSSFCPGSGRQNGGKNARIMLEVCRR
                     FDVPVVLGSDAHIWYDVGEMSRCRALLREVGFPPELVLNYSPEGLDFLLNRKRDGSAL
                     PVSGGASAGEA"
BASE COUNT          616 a          900 c          823 g          620 t
ORIGIN      
        1 caccgttccg atgacggagc ccacgatgta cacggcgaag gtgcccttgg tctcaggggc
       61 gtcaggaccg taaatgtctg tggtgaggcc caggttggaa tccctgttga tggagtagca
      121 ggccccgatg gcttctttct tcaggccgag aaggagcgcc accggcaggg agatgagaat
      181 ggttcccagg tttccgaact cctggagcag aagggccggt cccacctcca ccagcctggc
      241 caggttcgcc ccggccccca cgcccatctt cgccatgaag ggggcgaggc agacgagaac
      301 catagctccc gcggcgccgg actccttcat atcgaagagc ctcagcagat cgggcccgaa
      361 gagcccgccg atgatggtcg cgaagaccat ggggaaaagg gtcagcgttc cgatgccgat
      421 gtcgaacttc acgatgccga tcatatcgct gaggacagcc agaaccagcg cccccgcata
      481 cactttccag taccgcagca gccttccctt gatggatgat tccacgtcga tttcctccct
      541 gaaaaaaaat ggcccccgga acagggggtg ggcataaatg catcctgtac catgtcagac
      601 atggcaactc tggaatgata caccaaacat aaaaacatat caacgcctaa atataagtat
      661 ttttttcaac atatctcaga gaaatgaagg aattataccg cagctcgccc ctggaactca
      721 gaggagccgt ccattggaac agtatctcca cactgtccac ccttttgagg gttttcaccg
      781 tgctcccgcc ggagaaaaga acagcagaaa tacaacgaaa gaacaaaaaa gcttccggtt
      841 ccggatcacg ccgctctctc cttccgaaac tcctctgccg tcccgggctc ctccctgtct
      901 cttcacagct cccccagcgc ccccaggatg gccttccagt caaaggtccg ggccaggtgg
      961 aaaagttcgt cgtcaccgtt gatggaattc agggtgaaca cgatcgtctc gggtgcgctt
     1021 ctggaaaggc tgtagggagg cagaaagacg tacagcgtgg cctcggggac tttcctctcc
     1081 ggagagaccc tgtagacgat ctccgcatag cggatcccgt cgatgacccc ctgacgcatc
     1141 cgttccgacg aaacatcgag cgcagtttcc tcgaaatcgt cccttccgtc cgggtttccc
     1201 ggcatcttga gggcctgcac cacgaggttc ctgccctggt ggtactccct ggtgaacacg
     1261 gcggccctgc cctgtccgga atcgggcgtc acgatgggga ccatctccat gccttcccac
     1321 cctgggagat ccttgagaag gtgacgcatc ttctccgtga aaggctcggc atccgaagga
     1381 gaaacacaga aaagcagtat tgcagcggca aagaacagga acagcgtttt tttcatcccg
     1441 acagcccctt ccggatatct ggaattctga aatttcctgt attatatatg tccacccggg
     1501 atgcttttca actttccgga gcgtgttttt tccggtttcc gggaaagcgg ctgttttccg
     1561 ggccggcccg gaaacatcct gggatttcag atgaccggca aggcgggaag actgtccgga
     1621 agagggtgat ggcatgtttt ttatcaaaga cagagaaaag gccctgcgca gatacgattt
     1681 ctttctcatt tttctggcgg tcgcatcggt tgggctgatc atagccgagg aaagaatgga
     1741 tttcactgag gccgaagagg gaatcttcgg cctcgtggat acagccgtgt ggctgatctt
     1801 cgtgggagac tacttttcac ggctctttct cgcgggggac aaaaaactgt acgtgaaaac
     1861 ccacatcatc gaactcatcg ccatactgcc cttcgatgcc ctgttcaagg gtctccggtc
     1921 ccttcggctc ttcaggatgc tccgttcaac ccgggcgctt cggattctcc ggcttatcag
     1981 gctcgccgcc tacttcggca gggcccaccg gtacgcctcc cggttcctga ggcagcacaa
     2041 tttccagtac gtcctctttt tcacggtgct cacggtcctc atcggtgccg cggcagtccg
     2101 ctacttcgag cagatgggct tttacgacgc cctctggtgg gccttcgtga cggcgaccac
     2161 cgtggggtac ggcgacatct caccctccac ggcaggggga aggattgtgg cgatcatgct
     2221 gatgctggtg gggatcggct ttataagcac cctcaccggg accatcgcgt cctttttcgt
     2281 cgctcccgcc cctgagccgg aggcgaaacc gaagaacgaa tttctctccc tcgccctgca
     2341 gaggctcgaa aacttcgaga acctcagcac ggaagaagtc cgggagatct gcgccgtcct
     2401 ccaggggctc aaggaaaaac agtagttttt ccgtttccgg agtcatgcct ccccggcgga
     2461 tgcgcctccg gaaacgggaa gggcggagcc atcccgcttt ctgttcagaa gaaaatcaag
     2521 cccctccgga gaatagttga gcaccagctc cgggggaaag cccacctccc ggagaagggc
     2581 tctgcatcgg ctcatctccc ccacgtcgta ccagatatgg gcgtcgcttc ccagcaccac
     2641 cggcacgtcg aaccggcggc agacctccag catgatccgg gcgttctttc cgccgttctg
     2701 tcgccccgag cccgggcaga agctggagtt gttgatctcg agggccacct tctcccgctg
     2761 ggcggccgct accacctctt cgtagtcgag ggggtagcgg tcatcgtccg ggtggccgat
     2821 gatcttcacg taggggttcg ccatggcgcc gatcagggcc ctggtgttct gttccatggt
     2881 cccgaaggga atgcagggcg gatggaggct cgcgatggca tattccagct ttcccaggac
     2941 atcctccccc acgtcgagg
//
LOCUS       DAOTHD010000166         2951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_39743_length_2951_cov_2.955801, whole genome shotgun sequence.
ACCESSION   DAOTHD010000166 DAOTHD010000000
VERSION     DAOTHD010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39743_length_2951_cov_2.955801"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..710)
                     /gene="tsf"
                     /locus_tag="P5219_03890"
     CDS             complement(117..710)
                     /gene="tsf"
                     /locus_tag="P5219_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRX25930.1"
                     /translation="MEISAGAVKELRDRTGAGMMDCKHALAETGGDVEKAIDYLREKG
                     LAKAAKKASRAASDGRVFSYIHTNGKLGALVELNCETDFVAKTDEFQSLGHELCMHIA
                     AAAPQFLTSEEVTGEILEREREIYRQQALEEGKPANIVDKIADGKINKFYEVNCLMDQ
                     VWIRDGDKKIKDIVMETIAKLGENIVVRRFARFNIGE"
     gene            complement(733..1539)
                     /gene="rpsB"
                     /locus_tag="P5219_03895"
     CDS             complement(733..1539)
                     /gene="rpsB"
                     /locus_tag="P5219_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRX25931.1"
                     /translation="MAVVSMKQLLECGVHFGHQTRRWNPKMKPFIFTERNGVYIIDLQ
                     KTVRGLEKAYDYIREVAKNNGSVLFVGTKRQAQDTIREEAERCGQFYINQRWLGGLMT
                     NFPTIRKRVARMVELRKMEDEGNWAELPKKEVALLRKELGKLEKYLKGITNMKAIPDA
                     IFLIDPRREENAVLEARKLGVPVIAIVDTNCDPEVIDFPIPGNDDAIRAIKLIAGLMA
                     DAFIEGRQGEDAGVKMISSEDGEPVEVSSDNIIEVKERLHEVYDDSEEEA"
     gene            1755..2078
                     /locus_tag="P5219_03900"
     CDS             1755..2078
                     /locus_tag="P5219_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HRX25932.1"
                     /translation="MAKDSLIERLENLPQDRKAMLNRLRRVEGQLRGIQRMIIEEKPC
                     YDVLLQLSAARKAMQKACIEILKNYLQKCVHEAKAPDFDNLEKLIEALIEISPAKALS
                     GDGDE"
     gene            complement(2094..2193)
                     /gene="ffs"
                     /locus_tag="P5219_03905"
     ncRNA           complement(2094..2193)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="P5219_03905"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(2333..>2951)
                     /locus_tag="P5219_03910"
     CDS             complement(2333..>2951)
                     /locus_tag="P5219_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRX25933.1"
                     /translation="GKYEGHDVELAELLASELGVSVEFVPTAWPKLMDEYLAGSFDIA
                     VGGITRSLARMLKGDFLQPYAPNGKVALIRKTDREKFSSLEAMDVPETTVIVNPGGTN
                     EKFVNANFKKAKVVVHQSNAEIPAMIAEGKGDVMITEVYEAVVYSRKDERLYGAFTDK
                     PLTKISFMGFFIQKDDPDFLRVMHYLWNDARLRGDLDRLFDKWLK"
BASE COUNT          635 a          854 c          734 g          728 t
ORIGIN      
        1 cggagagttt caaaagaact cttttgaatc gcatacctat cccttccctt tcacgggcta
       61 gaaaacggaa tatagaaaaa aggcgaggct tttcgccccg cctttcgatg cagaaactac
      121 tcgcctatgt tgaatcgggc gaaccgtcgt acgacgatat tttcaccgag tttggcaatg
      181 gtttccatga cgatgtcctt gattttcttg tcaccgtcgc ggatccatac ctgatccatc
      241 aggcagttta cttcgtagaa cttgttgatc ttgccgtcgg ctatcttgtc gacgatattt
      301 gcgggcttgc cctcttcaag agcctgctgc ctgtagattt cccgctcccg ctcaagaatc
      361 tcgcctgtca cttcctcgga ggtgaggaac tggggggctg ccgccgcaat atgcatgcac
      421 agctcgtggc cgagactctg gaattcatcc gtctttgcga caaaatcggt ctcgcagttc
      481 agttcgacga gggcacccag tttcccgttc gtatggatat aggaaaacac ccttccgtct
      541 gacgcagctc tggaagcctt tttggcagct ttcgcgagac ccttttcacg aaggtaatcg
      601 atggcctttt ccacatcccc gcctgtctcg gcaagggcat gcttgcagtc catcatcccc
      661 gcacctgttc tatcgcggag ttccttgaca gctccggcgc taatttccat attcattgcc
      721 tccctttatg gcttaagctt cttcctcgga atcgtcgtac acttcgtgaa gacgttcctt
      781 gacttcaatg atgttgtcgg aggagacttc gacgggctcc ccgtcttcgg aggagatcat
      841 cttgacgccg gcgtcctcgc cctgccgtcc ctcgatgaac gcgtccgcca taagtccggc
      901 aataagcttg atggccctga tggcatcgtc gtttccggga atggggaagt cgatgacctc
      961 gggatcacag ttcgtgtcca cgatggcaat gacggggact ccaagtttcc tggcctcgag
     1021 aacggcgttt tcctccctgc ggggatcgat aaggaagatc gcatcgggaa tggctttcat
     1081 gttggtgatt cccttgaggt acttttccag ttttccgagt tctttcctca gaagggcaac
     1141 ttccttcttg ggaagctccg cccagtttcc ctcgtcctcc attttgcgaa gctccaccat
     1201 gcgggcgacc cgcttcctga tcgtcgggaa attggtcatc agtccgccga gccagcgctg
     1261 gttgatgtag aactgaccgc accgttccgc ttcttccctg atggtgtcct gggcctgcct
     1321 cttggtgcct acgaagagaa ccgaaccgtt gttctttgcg acctccctga tgtagtcata
     1381 ggctttctcc aggcccctga cagttttctg gaggtcgata atgtacactc cgttccgctc
     1441 cgtgaagatg aacggcttca tcttcgggtt ccacctgcgg gtctgatgac cgaaatgaac
     1501 gccgcactcc aaaagctgct tcatactgac tactgccaca ataacccctc ctgtaaaatg
     1561 tttttccctc cacaaacatc gcctggaacc gggaccatgc ctttcgggca cagcaacccc
     1621 cagccccatc agtttgtgtg tgtgatgagg accgaaagag tataccacaa ccggcagttg
     1681 accggcaact gtggaagaag tacgatatac cccgtatttc aacatgaaaa acaaacgact
     1741 gggggcattg agacatggca aaagactcac tcatagaacg gctggaaaac ctgcctcagg
     1801 acaggaaagc catgctgaac aggcttcggc gcgtcgaagg acagcttcgc ggaatccagc
     1861 ggatgatcat cgaggaaaaa ccctgctacg atgttcttct gcagctctcc gcagccagaa
     1921 aggccatgca gaaagcctgc atcgaaattc tgaagaacta ccttcagaaa tgtgtccatg
     1981 aagcgaaagc ccctgatttc gacaatctgg agaaactcat cgaggccctc attgaaattt
     2041 cccctgcgaa ggctctttcc ggggacgggg atgaataacc acagccgtct gcgggaaacc
     2101 cagctccccc gtggcacccg gaaaattctc cttactgctg ctcccttccg ggcctgacag
     2161 ggttcggagg tctccgccgc acgggactgg attccccgca gacggctgcg gggtgtcgaa
     2221 cccgaaaatt atagcacggc gtctttcatt ctgccactat cggatcatcc cataaaaaac
     2281 ggggaggcgg acatcccgct tccccgttcc agcgtacttt gagagtccgg agctatttca
     2341 gccacttgtc gaagagcctg tcaaggtctc cccgaagcct tgcatcgttc cagaggtaat
     2401 gcatcacccg gaggaagtcg ggatcatcct tctggataaa gaaccccata aaactgattt
     2461 tcgtcagggg cttgtcagtg aaggcgccgt agagtctctc gtccttccgt gaatacacca
     2521 cggcctcgta gacttctgtg atcatgacat ctcccttgcc ttcagcaatc atggcgggta
     2581 tttcggcgtt gctctggtga acgacgactt tggccttttt gaaatttgca ttgacaaact
     2641 tttcgttcgt tccgccggga ttcacaataa ccgttgtttc cggaacatcc attgcttcca
     2701 gggaagaaaa cttctctctg tcagtcttcc ggataagggc gactttgccg ttgggagcat
     2761 agggctgcag aaaatccccc ttcagcatcc gggccaggct tctggtgatt ccgcccacgg
     2821 cgatatcgaa ggacccggca agatattcgt ccatgagctt cggccacgca gtggggacaa
     2881 actccacgga gactccgagt tccgaggcga gaagttccgc aagctccaca tcgtgacctt
     2941 cgtatttgcc c
//
LOCUS       DAOTHD010000167         2939 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_39961_length_2939_cov_3.026006, whole genome shotgun sequence.
ACCESSION   DAOTHD010000167 DAOTHD010000000
VERSION     DAOTHD010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39961_length_2939_cov_3.026006"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(292..1197)
                     /locus_tag="P5219_03915"
     CDS             complement(292..1197)
                     /locus_tag="P5219_03915"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRX25934.1"
                     /translation="MKILVAEDDATSLFMLQSLLEKWGYETVALSDGVEALSVLNGED
                     PPFLAILDWMLPGLSGPEICRRLRMGEEARKGRPYQYIIMLTVKGEKENVVAGLDAGA
                     DDYVTKPFDSQELQMRIKVGERILLLQEQLRKAALYDALTGLPNRRAVIDGLEGEFAR
                     SERSGESFAIALLDLDHFKEVNDRYGHLTGDSVLAESARRIRSAVRSYDLVGRYGGEE
                     FLLVFPGLKGDDALRICERVCSVIEKKPFFPVPQQGSGTPFTVTASLGICEWNPSSSG
                     PDEMLMGADQALYRAKRKGRNAVCL"
     gene            complement(1206..>2939)
                     /locus_tag="P5219_03920"
     CDS             complement(1206..>2939)
                     /locus_tag="P5219_03920"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX25935.1"
                     /translation="MGMFMGYLGEDDADILDLSFAFLTVVLSTAAGLLQNAELYTVIR
                     GLNSELSKKIERLEESERSLAEAMRARDTFLANVSHEIRTPLNGIIGMASLLEDTALD
                     SRQADMVRILRDESSSLLRLINDLLDFSRIEAGKLSFEDAPFDFPEFWNSIRDSFLPR
                     ARQKNLRFRMDLTGQVPAAVSGDSLRIRQVFGNIIGNALKFTSKGEVSVLGDILPGDG
                     RQCLLKVDVRDTGIGISAEQAGRLFRPFVQGDPSTTRKYGGTGLGLVISKRLVEGMGG
                     TISIEPEDEQGAHFRFSLPLKILPGKTREREAAGTAIVFPPGYSVLVVEDNETSRMVA
                     VSLLEKMGVPVIETAENGAVAIEKLSSRRYDLVLMDIQMPVMDGLEAVAAIRDSSSPV
                     LDREVPVAAMTANTLPADRGKYLRAGMTDYISKPVLPEELARLLLKNLGVENLRNADW
                     DSGNEKSIFRKDLLLKRMGGDEKLCERTIQLFVEDSLKLAEDLAALIQSGDFDEAKMK
                     AHTLRGAAANVESREIMAAAAAVEEAAAAGKGSEARALMPDVFAARTRFLAEVQGSED
                     KHRKSAGEWKG"
BASE COUNT          604 a          875 c          800 g          660 t
ORIGIN      
        1 cccattttcc gcgaaaccag agagcgtttg cagcaagaga aagctctctc ggggaaagtg
       61 agccgctttt ttgcgccagc agtgtatcca cacttctcca gtccctggcg gaaaaaagtt
      121 cgtccatgcc ggaagctgcc cctgccggaa aagcagggag gaagaagacg atgattaaaa
      181 tacagaggac agcgggtgct ttcagcaatc cgggcaccgg catcactcct ttcacatttt
      241 tcggggatta taggggaagg gcgggaataa atctccggta aaactcccta atcagaggca
      301 gacggcgttg cgtcccttcc tcttcgcccg gtagagagcc tggtcagccc ccatgagcat
      361 ttcatcaggc ccgctggagg aggggttcca ttcacatatg ccaagactgg cggtcacggt
      421 aaagggagta ccgctcccct gctgcggcac agggaaaaag ggtttttttt ctattacgga
      481 acataccctt tcgcagatcc tgagagcatc gtcacctttc aggcccggaa aaacaagaag
      541 gaactcctcc ccgccgtacc tgcccacgag gtcgtagctc ctcaccgccg agcggatccg
      601 gcgggccgac tccgccagga cggaatcgcc cgtcaggtgg ccgtacctgt cgttgacctc
      661 cttgaaatga tcgaggtcca gaagggcgat ggcaaatgat tccccggacc tctcggaacg
      721 ggcgaactca ccctcgaggc cgtcaatgac agccctcctg ttggggagac ctgtgagcgc
      781 atcgtagagg gccgccttcc tgagctgttc ctggagaagc aggattcttt ctccgacctt
      841 tatccgcatc tgcagttcct gggaatcgaa gggctttgtc acataatcgt ccgctcccgc
      901 atcgaggccg gcaacgacgt tttccttctc ccccttgacg gtcagcatga tgatgtactg
      961 gtacggcctt ccctttcgtg cctcctcgcc catgcggagc ctccggcaga tctcgggccc
     1021 gctcaggccg gggagcatcc agtcaaggat cgcaaggaag ggcggatctt caccgttcag
     1081 caccgaaaga gcttccacgc cgtcggaaag ggctacagtt tcgtaccccc atttttcgag
     1141 cagggactgc agcatgaaca aagatgtcgc gtcatcttcg gcaacaagaa ttttcatagg
     1201 gtcattcatc ctttccattc tccggcgctt tttcgatgtt tgtcttcact gccctgaact
     1261 tcggcaagaa atcgtgtccg ggccgcaaaa acgtccggca tgagggctcg ggcttccgac
     1321 cctttgcctg cggcggcggc ctcttccacc gctgccgctg cagccatgat ttccctggat
     1381 tccacgttcg ccgccgcgcc ccgaagggta tgggccttca tcttcgcttc gtcgaaatca
     1441 cccgactgga taagggcggc gagatcctcc gcaagtttaa gggaatcctc aacgaaaagc
     1501 tgtatggtcc gttcgcagag tttttcatcc ccccccatac gtttgaggag gagatccttt
     1561 ctgaagatgc tcttttcatt cccgctgtcc cagtcggcat tccgaagatt ttccactccg
     1621 aggtttttca gaagaagtct cgcgagttcc tcagggagaa ccggcttgct gatgtagtcc
     1681 gtcatgcccg ccctgagata ttttcccctg tcggcgggaa gggtgttggc cgtcatggcg
     1741 gctacgggaa cctcccggtc caggacgggg gacgaggaat ccctgatggc agccacggct
     1801 tccaatccat ccatcaccgg catctggata tccataagta caagatcgta ccttctcgat
     1861 gaaagttttt caatggccac ggcaccattt tccgcagttt ctatcaccgg gacgcccatt
     1921 ttttcgagca gcgaaaccgc caccatcctg cttgtttcgt tatcctccac cacgagcacc
     1981 gaatacccgg gagggaaaac tattgcggtt ccggcagcct ccctttcgcg ggtctttccg
     2041 ggcaggatct tcagtggaag ggagaaccgg aaatgggctc cctgctcatc ctcaggctcg
     2101 atggatatcg ttcctcccat gccctccacg agtcgcttcg agatcacgag gccgagaccg
     2161 gttccgccgt attttctcgt agtggagggg tccccctgga caaaggggcg gaagagccgg
     2221 cccgcctgtt ctgctgaaat gccgatcccc gtgtcccgaa catccacctt gaggagacac
     2281 tgtctcccat ccccggggag aatgtcgccg aggacggaaa cctccccctt tgaagtgaat
     2341 ttcagggcat tccctatgat attgccgaaa acctggcgta tcctgagaga atctccggaa
     2401 acagctgcag gaacctgtcc cgtgaggtcc atcctgaacc ggagattttt ctgccgtgct
     2461 ctcggaagaa agctgtcccg gatgctgttc cagaattccg gaaagtcgaa gggggcatcc
     2521 tcgaagctga gctttccggc ctcgatcctt gaaaagtcga ggagatcgtt gataagcctg
     2581 agcagtgacg atgattcatc cctgaggatc ctgaccatgt ctgcctggcg ggagtcaaga
     2641 gcggtgtcct cgagcaggct cgccatgccg ataattccgt tcaggggggt tcgtatttcg
     2701 tggctcacgt tggcgagaaa ggtatccctg gccctcatgg cctcggcaag actccgctca
     2761 gattcttcga gccgttcgat tttcttgctg agctcgctgt tcaggccccg gatcacagtg
     2821 tacagttctg cgttctggag cagtccggct gccgtgctga ggaccacggt gagaaaggcg
     2881 aaggaaaggt cgagaatatc ggcgtcgtct tctcccaggt atcccatgaa catccccat
//
LOCUS       DAOTHD010000168         2935 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_40035_length_2935_cov_3.338194, whole genome shotgun sequence.
ACCESSION   DAOTHD010000168 DAOTHD010000000
VERSION     DAOTHD010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40035_length_2935_cov_3.338194"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..1114
                     /locus_tag="P5219_03925"
     CDS             101..1114
                     /locus_tag="P5219_03925"
                     /inference="COORDINATES: protein motif:HMM:NF027559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4892 domain-containing protein"
                     /protein_id="HRX25936.1"
                     /translation="MFSRRGSCLGFLLVFLMSFFPVSPAPGADIKGGKDSPLLRRYEG
                     SFIVQYSAKEYDVTVIPLGKTEYRGDRYVFTASEKAEGRTARLLYVAPAGRSALEVFR
                     NYEGELKEKGYEILFSGSKDELGPYDSFAETLYGRDRQYPIPGDERTKNQQFLSARLV
                     RTEGNVYVTVCAFENNFWGSETKMEKGRTYCRVDMVETKPMETKMVTVTSEEMARGLE
                     SSGKIALYGIYFDTDKADIKPESKPALEEMAKLLKAFPDLRVLIVGHTDSTGDREYNM
                     GLSRRRAEAVVKSLRESYGIAASRLVPAGVGMLAPVASNRTEEGRAKNRRVELVEMQP
                     SLQ"
     gene            complement(1106..2080)
                     /locus_tag="P5219_03930"
     CDS             complement(1106..2080)
                     /locus_tag="P5219_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25937.1"
                     /translation="MKPILSSFALIASGLLLGYGIRRLFEKGFLGPGERLDRLRSFLQ
                     RLVLLGLGPVTFVGALWIVEIREPKIALLALIGAFCHIFGGGAAALIARLFRMEPPQA
                     GAFFCCGFFTNVGAIGGLVIFLFLGEEGYAMVPLFKLLEELVYYGIGFPAARLYSAGA
                     KGRADGSLVPRLLRDPFLRVAVGGLVLGAALNLSGIPRPPMYSSLNSLLIPLSTVLLL
                     ASIGMAMRFGKTALYLKEGFTIIFLKFLLVPAACVSLAALAGLNGIAGGLPLKAVLVL
                     SSMPVAFNALVPPSLYGLDLDLANSCFLVSVLGLAALLPALHFALDLL"
     gene            2257..>2935
                     /locus_tag="P5219_03935"
     CDS             2257..>2935
                     /locus_tag="P5219_03935"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRX25938.1"
                     /translation="MGRPVIGIVGNLLVEQGGFSPGMERSYVNNDYVAAVERAGGIPL
                     ILPVVRGQDVAERQTECADALLISGGYDIDPLLYGEEPHRALEFVYRDTDIFQLRTIK
                     TALAKGQPLLAICKGIQLLNVACGGTIYQDLDEIPDSFVKHSQNRKRSCPSHTVVAEE
                     GSVIAELLGTSFPVNSYHHQAVKDVGRGLKVTSRAMDGVAESLEMEGEPFVVGVQWHP
                     EMMMSGGD"
BASE COUNT          614 a          830 c         1005 g          486 t
ORIGIN      
        1 cggtacgtac ggtggtgtga gaggacgggg gctagccgcc ccctcctact cgattcggct
       61 ataatatgcg gacaattctg agattgcagg agggggtttc atgttctcgc gtcgaggcag
      121 ctgtctgggg ttccttctcg tgttcctcat gtcgttcttc ccggtttcac ccgctcccgg
      181 ggcggatatc aagggcggaa aggattctcc tctgctccgc cggtacgagg ggtccttcat
      241 cgtccagtat tcggcgaagg agtacgatgt tacggtcatc cccctgggga agacggaata
      301 ccggggcgac cgctatgtct tcaccgcctc ggagaaggcg gagggcagga ccgcgcggct
      361 tctgtacgtc gccccggcgg gacggagcgc cctggaggtg ttccggaact acgaggggga
      421 gctgaaggaa aaggggtacg agatcctgtt ttccggttca aaggacgaac tcggccccta
      481 tgacagcttt gccgagaccc tgtacggacg ggacaggcag tatcccatcc ccggggacga
      541 gaggacgaaa aaccagcagt tcctctcagc ccgccttgtc cgcacggaag gaaacgtcta
      601 cgtgacggtg tgcgctttcg aaaacaattt ctggggctcg gagacgaaaa tggagaaggg
      661 gcggacctac tgccgggtcg acatggtgga gacgaagcca atggagacga aaatggtgac
      721 ggtcacgtcg gaagagatgg ccaggggcct ggagtcctca ggaaaaatcg ccctctatgg
      781 tatttatttc gacaccgaca aggcggacat caagcccgag tcgaagcccg ccctggagga
      841 aatggcgaaa ctcctgaaag ccttcccgga tctccgggtg cttatcgttg gccacacgga
      901 cagcacgggg gaccgggagt acaacatggg cctttcccgg agacgggctg aggctgtggt
      961 gaaatccctc cgggagagct acggaatagc cgcttcacgg ctcgttccgg caggggtggg
     1021 gatgctcgcc ccggtggcgt ccaacaggac ggaggagggc cgggccaaga accgcagggt
     1081 ggagctcgtc gaaatgcagc cgtccctaca ataggtccag ggcgaagtga agggcgggca
     1141 gcagggcggc cagaccgagc accgagacca ggaagcagct gttcgccagg tccaggtcca
     1201 ggccgtacag ggacggcggg acaagggcgt tgaaggcgac cggcatggag gaaaggacga
     1261 ggacggcctt cagagggagg ccgccggcga tgccgttcag gcccgccagt gccgcgaggg
     1321 agacgcaggc cgccgggacg agaagaaatt tcagaaagat gatggtgaac ccctccttca
     1381 ggtagagggc ggttttcccg aacctcatgg ccatgccgat ggaagcgagg aggagcaccg
     1441 tgctgagggg aatgagcagg ctgttcaggg aggaatacat tgggggcctc ggaatgcccg
     1501 acaggttgag ggctgccccg aggaccagtc cgccgacggc caccctgaga aaggggtccc
     1561 ggagaaggcg ggggacgagg cttccatccg ccctgccttt cgctccggcg ctgtagagcc
     1621 gggcggcggg gaaaccgatg ccgtaataga cgagttcctc caggagcttg aaaaggggga
     1681 ccatggcgta tccctcttcc cccagaaaca ggaaaatcac cagtccgccg atggccccca
     1741 cgttggtgaa gaagccgcag cagaagaagg ctcccgcctg ggggggctcc attctgaaca
     1801 gcctggcgat gagtgccgcc gccccacccc cgaaaatgtg gcagaaggcc cctatgaggg
     1861 cgaggagggc gattttcggc tccctgatct ccacgatcca gagggctccc acgaaggtca
     1921 cgggcccgag acccagcagt acgaggcgct gcaggaagga ccggagacgg tcgagccttt
     1981 cgccgggacc gaggaaaccc ttctcgaaca gacggcggat tccgtacccg agcagcaggc
     2041 ctgacgctat cagggcgaat gaggacagga tgggcttcat ggcggcgact ctcctccggc
     2101 gggagcttgt ccgggctgca tccggcccgt tttcccacga tcatgggggg tattattctc
     2161 ctccctcccg gcgatgtcaa cttgaaaaag acatccccgg cgctatactt ggatcaggcg
     2221 gaagtccgcc gatccgtaca acaggaggaa ttgatcatgg gccgaccggt gatcggtatt
     2281 gtggggaacc tgcttgtgga gcagggaggt ttttccccgg gcatggagag aagctacgtg
     2341 aacaacgact atgtggccgc cgtggagcgg gccgggggca tccctctcat cctgccggtg
     2401 gtgagggggc aggatgtggc ggagaggcag acggagtgcg ccgacgctct tctcatatcg
     2461 ggagggtatg acatcgatcc gctgctgtac ggcgaagagc cccacagggc cctggaattc
     2521 gtctaccggg acaccgacat tttccagctc cgcaccatca agacggcctt ggcgaagggg
     2581 cagccccttc tggccatctg caagggaatc cagctcctga acgtggcctg cgggggcacc
     2641 atctaccagg atctcgacga aattcccgat tccttcgtaa aacattccca gaaccggaag
     2701 cgatcctgcc ccagccacac ggtggtggcc gaggaggggt cggtgatcgc cgaactcctt
     2761 ggaacatcct tccccgtgaa cagctaccat caccaggcgg tgaaggacgt gggacgggga
     2821 ctgaaggtca cgtcccgggc catggacggc gtggcggaat ccctcgagat ggagggggag
     2881 cccttcgtgg tcggggtcca gtggcacccc gagatgatga tgtccggagg ggacg
//
LOCUS       DAOTHD010000169         2929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_40162_length_2929_cov_2.339596, whole genome shotgun sequence.
ACCESSION   DAOTHD010000169 DAOTHD010000000
VERSION     DAOTHD010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40162_length_2929_cov_2.339596"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..406
                     /locus_tag="P5219_03940"
     CDS             161..406
                     /locus_tag="P5219_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HRX25939.1"
                     /translation="MPLNELAVPERVTGERQVRRKLEAGRLAKLFIAEDADPAMVGPL
                     EAEARRQDIPVERVSDMVRLGRACAISRGASVAGIKR"
     gene            456..827
                     /gene="rpsL"
                     /locus_tag="P5219_03945"
     CDS             456..827
                     /gene="rpsL"
                     /locus_tag="P5219_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRX25940.1"
                     /translation="MPTINQLIRHGREEKRRKTDAPALQGNPARRGVCTRVYTVTPKK
                     PNSALRKVARVRLTNGIEVTSYIPGIGHNLQEHSVVLVRGGRVKDLPGVRYHIIRGTL
                     DCGGVENRKRSRSKYGARRPK"
     gene            870..1340
                     /gene="rpsG"
                     /locus_tag="P5219_03950"
     CDS             870..1340
                     /gene="rpsG"
                     /locus_tag="P5219_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRX25941.1"
                     /translation="MPRKGHVKKRQALPDTKFGSAALAKFINSLMRKGKKSVAEKIMY
                     RALDIAGEKLNTEPFEVFKKAMENVRPLVEVRPRRVGGATYQVPVEVPAERAQVLCIR
                     WILGYSRAKKGMPMAERLAREFMDAYKGEGSSIKKKEDTHKMAEANRAFAHYRW"
     gene            1404..>2929
                     /gene="fusA"
                     /locus_tag="P5219_03955"
     CDS             1404..>2929
                     /gene="fusA"
                     /locus_tag="P5219_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRX25942.1"
                     /translation="MQTLDMKRIRNIGIAAHIDAGKTTTTERILFYTGKNYKMGETHE
                     GAATMDWMDQEKERGITITSAATTCVWGDNFINIIDTPGHVDFTVEVERSMRVLDGAV
                     AVFCAVGGVEPQSETVWRQADKYHVPRVAFVNKMDRIGADFDNVVRGIKEKLGARPVP
                     IQLPIGIEDSFTGMVDLVEFRGIQYSDDLGTDPHVVPVPSSMEEEAKAARDAMIEILA
                     DYDEEIMGLYLDGRQVPVELVKKALRAGTVGLKIVPVICGSAFKNKGVQMLLNAVVDY
                     LPSPVDLPPVTGVNPDSMEEETRASSITDPFTALAFKIMVDPFVGRLVFTRVYSGKLT
                     KGSTVFNATNNGRERVGRILRMHANKREELEEAEAGMIVALPGLKATRTGDTLCEEGK
                     PVVLENLVFPEPVISLSVEPATKNDKLKLTKGLVALAEEDPTFRVKTDEETSQTIISG
                     MGELHLEIIVDRLKREFGVDVKVGRPQVAYREAVRNSSRAEGKYIRQSGGRGQYGHVV
                     "
BASE COUNT          691 a          784 c          903 g          551 t
ORIGIN      
        1 gccaggggaa ggtagccgct ggaaaaatga ccgaaacggc cggccgggaa taagaagaaa
       61 agaaatccgg agagaaaaca ggggcgggaa aagtccggct tgacacctat cccgcccttt
      121 tgttattatt ccttggtgct tttgcacagg agggattctt ttgcctttaa acgagctggc
      181 agttcccgag agagtcacgg gagagcgtca ggtgaggcgg aagcttgaag caggcaggtt
      241 ggcgaaactt ttcatcgccg aggatgccga cccggccatg gtcggtcctc ttgaggcgga
      301 agcgcggaga caggatatcc ccgtggaacg agtcagcgat atggtccggc tgggcagggc
      361 gtgcgccata agccgcggag cttctgtcgc ggggattaaa agataaacgg gccttcaagc
      421 ccgatagaaa agaacaaatg gaaggaggaa gttgtgtgcc gactattaac cagcttatcc
      481 ggcatggccg cgaggaaaag aggcggaaaa ccgatgctcc tgcactgcag ggaaatcctg
      541 cgcggagggg tgtctgcacc cgtgtgtaca ctgtaacccc caagaagccc aactcggctt
      601 tgagaaaggt tgcccgtgtt cgcctcacca acgggatcga ggtaacatcc tacatccccg
      661 gaatcggcca taacctgcag gagcactccg ttgtgctcgt caggggcggg cgagtgaagg
      721 accttcccgg tgtcaggtat cacatcatcc ggggaaccct tgactgtggg ggcgtggaga
      781 atcgcaagag atcccgttcc aagtacggcg ccaggagacc caagtagggt ttgcggcgtt
      841 tgagcttttt tttgaggagg ggttgaccta tgccgcgtaa ggggcatgtc aagaagcgtc
      901 aggctctgcc ggataccaag ttcggcagcg ccgcactggc aaagttcatc aacagcctga
      961 tgcggaaagg caaaaagagc gttgcagaaa agattatgta ccgggctctc gatattgccg
     1021 gggaaaaact gaataccgaa ccctttgagg ttttcaagaa ggccatggaa aatgtccggc
     1081 ctctcgttga agtgcgtccc cgcagggtcg gcggcgcaac ctaccaggtt cccgtggaag
     1141 ttccggccga gcgggcccag gttctctgca tccgctggat tctcggctat tcgcgggcaa
     1201 aaaagggtat gcccatggcc gaacggcttg cgagagaatt tatggatgcc tacaagggtg
     1261 agggcagctc cataaaaaag aaggaagaca cccacaagat ggcggaggca aaccgcgcgt
     1321 tcgcccacta ccgctggtag ttcgcaacga agcgcaggaa tttgcatgcg gcaaactgat
     1381 ggaaacgagg cttggtggta gctatgcaga cacttgatat gaaaagaatc cggaatatcg
     1441 ggatcgccgc ccacatcgac gcgggcaaga cgaccaccac cgagcggatt cttttctaca
     1501 ccggaaagaa ctacaaaatg ggcgaaaccc atgaaggcgc ggccaccatg gactggatgg
     1561 atcaggagaa ggagcggggg atcaccatta cctccgccgc cactacgtgt gtatggggag
     1621 ataattttat caatataatt gatacgcccg gccacgtgga cttcacagtg gaagtcgagc
     1681 ggtccatgag agtgcttgac ggagcggttg ccgtcttctg cgctgtgggg ggggtcgaac
     1741 cccagtcgga aaccgtctgg cgtcaggcgg acaagtatca tgttcccaga gtcgccttcg
     1801 tgaacaagat ggaccgaatc ggagccgatt ttgacaacgt cgtccgggga atcaaggaga
     1861 agcttggagc caggccggtt cccattcagc tccccatcgg cattgaagac agtttcaccg
     1921 gcatggtgga tctcgtcgag ttccggggta tccagtattc cgacgatctc ggcaccgacc
     1981 cccatgtggt gccggttccc tcttccatgg aggaagaggc gaaagcggcc agggacgcca
     2041 tgatcgaaat tctcgccgat tacgacgagg agatcatggg gctctatctc gacgggcggc
     2101 aagttcccgt agagcttgta aagaaagcgc tccgggccgg taccgtgggc ctgaaaatcg
     2161 tccccgtcat ctgcggctcc gccttcaaga acaagggagt ccagatgctg ctgaatgccg
     2221 tggtggacta tctcccgagc cctgtggacc ttcctcccgt gaccggggtg aaccccgact
     2281 ccatggaaga ggaaacccgg gccagcagta tcactgatcc ttttaccgcc ctcgccttca
     2341 agatcatggt ggatcctttc gtcggccgcc ttgtgtttac ccgggtctac tcgggaaaac
     2401 tgaccaaggg ctccactgtg tttaacgcta cgaacaacgg cagggagcgg gtcggccgca
     2461 tacttcggat gcacgccaac aaacgggagg agctggaaga agccgaggcc ggcatgattg
     2521 tcgctcttcc cgggctcaag gccacgcgca ccggcgacac gctctgtgaa gaaggaaaac
     2581 ccgtggtgct cgagaacctt gtgttcccgg agcccgttat ctccctctcc gtggagcctg
     2641 ccacaaagaa cgacaagctc aagcttacca agggcctcgt ggcgctcgcg gaagaagatc
     2701 cgaccttcag ggtgaagacc gacgaggaaa cttcccagac catcatttca ggcatgggag
     2761 agcttcacct tgaaatcatc gtggaccgcc tcaagaggga gttcggcgtt gacgtcaagg
     2821 tcggccgccc ccaggttgcg taccgcgagg cggttcgcaa ttcctccagg gccgagggaa
     2881 agtatatccg ccagtccggc ggacggggcc agtacggaca cgtggtttt
//
LOCUS       DAOTHD010000170         2911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_40467_length_2911_cov_3.384804, whole genome shotgun sequence.
ACCESSION   DAOTHD010000170 DAOTHD010000000
VERSION     DAOTHD010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40467_length_2911_cov_3.384804"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="P5219_03960"
     CDS             complement(<1..994)
                     /locus_tag="P5219_03960"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRX25943.1"
                     /translation="MRTLRSQIRWIMIAIVVLFVLSIFGMYGFDSGPRRGSSGGEDYI
                     VAEIDGKAVMRSMLDEQLRNYVERANIRDVTSADAPRLYQAALENIALVSRLATEAEE
                     SGLKATEEEINAAVKEVSEQFPTKEAFMQYLDRSGIKMSAFRESMAQQVVQMKLMEKA
                     VGTTDVKEEEVKEFYEKLKLLFFHSPAGYSMDFIRLKTKDEADKLQSVLAEGKEWKDA
                     VGAVTSGDVIEKTPESGPVFIPESSFKDALAPLAGLAIGEVSPALEIASNDILIAVKR
                     EKVEEKTAPFEEVSGDVKALLTEEKNREAQNNFFTGLRERTNIVIHDHELFPKTE"
     gene            complement(1074..>2911)
                     /locus_tag="P5219_03965"
     CDS             complement(1074..>2911)
                     /locus_tag="P5219_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HRX25944.1"
                     /translation="AFRSRELVMLLCDTKENLGDFLPVEADREGFLEKCKALGLQKIA
                     EKMVPGVLRDRKRPEPPAEESSGRGGRESAVIPAGVLIPVINGKYPVALALEEFCLTG
                     PDGEIAGKNREDALSALGRRGTGKVLLPDYKEAAACLGSSFLPSSAVLDFKTAHYLLH
                     PDSGVHHPEGLFTEYASLSPAEKGRFLAARFEELADEMGDHEGLSTLMEQTDLPLVPV
                     LVDMEKHGIGCSIPAFGALETDLSSRLKGIEEEITARGGEKINLNSPKQVAWLLFEKL
                     GLPTGKTTKTGYSTDISVLESLSAMGKPFDEVPNLLIEFRELSKMLSGFVQPLVKAAK
                     EGDGIIHGVFEPAVTGTGRLSSRDPNLQNLPSFGEWSGRIKKGLLPTGEGNVFVAADY
                     SQIELRVLAHLCGDRKLLDAFAKGRDIHTETASWVFSTEPSLVTPELRRVAKMINFGL
                     LYGMSSFGLAQRLGIGRQEASDIIRRYFDALPGVKEYLEKSYDDAKKRGYTLTLAGRI
                     RPLGEVSVSPRDRDALRRVAVNTPIQGTAADIARKAMVDFAAVFPTSGTVRLVLQVHD
                     SLVCECPAGDAEEVKRSLEEIMERAADLAVPLKVESKTGLSLAEV"
BASE COUNT          540 a          871 c          828 g          672 t
ORIGIN      
        1 cctcagtttt ggggaagagt tcgtggtcat ggatgacgat attcgtccgc tccctgagtc
       61 ccgtaaagaa attgttctgg gcttcccggt tcttctcttc cgtaagaagg gccttcacgt
      121 caccgctgac ctcttcgaag ggagcggtct tctcctccac cttctcccgt ttcacggcga
      181 tgaggatgtc gttgctggcg atctcaaggg cggggctcac ctcgccgatg gcaaggccgg
      241 caagaggggc cagggcgtcc ttgaagctgg actcggggat gaagacgggc ccgctttccg
      301 gggtcttctc gatcacgtcg cctgaagtca ccgcccctac ggcatctttc cattcctttc
      361 cttcggcaag aacggactga agcttatcgg cctcatcctt cgtcttcagg cggatgaaat
      421 ccatggagta ccctgcggga gaatggaaga agagaagctt cagcttctcg taaaactcct
      481 tcacttcctc ttccttgaca tcagtggtgc ccacggcttt ttccatgagc ttcatctgga
      541 cgacctgctg ggccatgctt tcacggaaag cgctcatttt gatgcccgaa cggtcaagat
      601 actgcatgaa ggcttccttg gtcgggaact gctcgctgac ttcctttacg gcggcgttga
      661 tttcctcttc cgtcgctttc agtccggatt cttcggcctc ggtggcaagc cgggagacaa
      721 gggcgatgtt ctccagggcc gcctggtaga ggcggggggc atcagcggaa gtgacgtccc
      781 tgatgttcgc cctctccacg tagttgcgga gctgttcgtc gagcatggag cgcattacgg
      841 ccttgccgtc tatttctgca acgatgtagt cttctccgcc ggatgatcct cttcggggac
      901 cactgtcaaa gccgtacatt ccaaaaatgg acaggacgaa aaggacgacg atggctatca
      961 taatccagcg aatctgagac ctcagggttc tcatcaacaa gggacatctt cctctctttc
     1021 tgatttccgg actccaatgg ggtattttac catagtatga ttcaaatgag acgtcagact
     1081 tcggcgaggc tcagacccgt tttcgactcc accttcaggg gcacggcaag atccgccgcc
     1141 cgctccatta tttcctcgag ggaccgtttg acctcctccg cgtcgcctgc agggcattcg
     1201 cagacaagag agtcgtgaac ctggagaaca aggcggaccg ttccggaagt cggaaagact
     1261 gccgcgaagt ccaccatggc ctttctcgca atgtcggctg ccgttccctg gatgggcgtg
     1321 ttcaccgcca ccctccgtag ggcatccctg tccctggggc tcacggacac ttcgcccagg
     1381 gggcgtatcc tccccgccag ggtcagggtg tatccccgct tcttcgcgtc gtcgtagctt
     1441 ttttcgaggt actccttcac ccccggcaag gcgtcgaaat accggcggat gatgtccgaa
     1501 gcctcctggc ggccgatgcc cagtctctgg gcaaggccga aggagctcat gccgtaaaga
     1561 agcccgaagt tgatcatctt tgcgacccgg cggagttccg gcgtgacgag ggacggctcc
     1621 gttgagaaga cccaggacgc cgtctccgtg tggatgtccc ttcccttggc gaaggcgtcc
     1681 agcagtttcc tgtcgccgca gagatgggcc agcacacgga gctcgatctg ggaatagtcc
     1741 gccgcaacga aaacatttcc ctcaccggtg gggaggaggc cttttttgat cctcccggac
     1801 cactcgccga aggaaggcag gttctggaga ttcggatccc tgctgctgag ccttccggta
     1861 ccggtcaccg cgggctcgaa cactccgtgg atgattccgt ctccctcctt tgccgccttc
     1921 acgaggggct ggacgaagcc tgagagcatc ttcgacagtt ccctgaactc gatgaggaga
     1981 ttgggcacct cgtcaaaggg ctttcccatg gcggaaagac tttcgaggac ggaaatatca
     2041 gtggaatacc cggttttggt tgtttttcct gtcgggagcc ccagtttttc aaagagaagc
     2101 catgccacct gcttcgggga attgaggttg atcttctccc ctcccctggc ggtgatctcc
     2161 tcctctatgc ccttcagcct tgaagagagg tccgtctcaa gtgcgccgaa cgcggggatg
     2221 ctgcagccga tgccgtgctt ttccatgtca acgaggacag gaacgagggg aaggtccgtc
     2281 tgctccatga gggtggaaag cccttcgtgg tcccccatct cgtctgcgag ttcttcgaac
     2341 ctggcggcga gaaaccgtcc cttttccgca ggagaaaggg acgcgtactc ggtgaacagc
     2401 ccttcggggt ggtggacgcc gctgtccggg tgcagaaggt agtgggcggt cttgaaatcc
     2461 agaacggccg aagagggaag aaaggagctt ccaagacagg ccgccgcctc tttgtagtcc
     2521 ggaagcagca ctttccccgt tccccgtctg ccgagggcgg aaagggcgtc ttccctgttt
     2581 ttcccggcta tttccccgtc cggcccggta aggcagaact cctcaagggc aagggccacg
     2641 gggtatttgc cgttgatgac gggaataagc accccggccg gaatgactgc gctttccctt
     2701 ccgcccctcc ctgaggactc ttccgccggg ggctccggtc tcttccggtc cctcagaact
     2761 ccgggtacca ttttttctgc tatcttctgc agtccgaggg ctttgcactt ttcaagaaat
     2821 ccttcccggt cagcctccac tggaagaaaa tcgccgaggt tttctttcgt gtcgcagaga
     2881 agcatcacaa gttccctgct ccggaaagcc t
//
LOCUS       DAOTHD010000171         2904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_40589_length_2904_cov_3.779221, whole genome shotgun sequence.
ACCESSION   DAOTHD010000171 DAOTHD010000000
VERSION     DAOTHD010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40589_length_2904_cov_3.779221"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..404
                     /locus_tag="P5219_03970"
                     /pseudo
     CDS             27..404
                     /locus_tag="P5219_03970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
     gene            complement(495..1136)
                     /locus_tag="P5219_03975"
     CDS             complement(495..1136)
                     /locus_tag="P5219_03975"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HRX25945.1"
                     /translation="MNEFTPWHNEALGKKVVESLKKNGFEAEYCSSAAEAADKILALI
                     PESASVGFGGSWTVKALGIQEKLAGKGNTILDHGAPGLSNEERQDVRKKQLTCDVFLS
                     GTNAVTLDGRLVNRDGNGNRVAAMIFGPGKVIVVMGTNKIVTDLDAAEERIQMIAAPI
                     NNKRIGLPNPCTQTGLCMDCQTQTRICNVTTIISKRPRSTPFHVFVIGEELGF"
     gene            complement(1133..2053)
                     /locus_tag="P5219_03980"
     CDS             complement(1133..2053)
                     /locus_tag="P5219_03980"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HRX25946.1"
                     /translation="MKKKIFALAVLLVLLVLSGSFYILRADEQAVVLRLGKVNATRAE
                     PGLYFKLPFIDQLTRYSKKLVEYDADPVAVVTSDKKNLVFDTFALFRISDPETFFRRV
                     RTVSSVQQRLDDSIYSAVRIVSGRLTLDELVKDKRQQAIESSTAIAREQSKEYGVEIL
                     SVAYKRVFLPQDNEQAVYRSMQAERNRVAGQLRAEGQAEGITRRSLADRKEVEMIAEA
                     RRKAEEIKGQGDSIAQDTLGKATAEAKDLYLFIKTMDFYQTALPGTPILLRPGEGILK
                     YLKGVGTQPGRNELKNGARPGQPQKEEEEE"
     gene            complement(2066..>2904)
                     /gene="hflK"
                     /locus_tag="P5219_03985"
     CDS             complement(2066..>2904)
                     /gene="hflK"
                     /locus_tag="P5219_03985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HRX25947.1"
                     /translation="GVADQGINFKLPVIDQVTIVNTEQIQRFEYGYRTVNQGPPATFS
                     DRPEESKMLTGDNKIVEIDWVLQYQAGDPVSYVVNLPEDRSYREKMIRDVAESTLREV
                     IASRKLDDVLTTEKEASQTEAKKLIQEKLDFLKTGVFIVAVQFQDVNPPKAVQAAFSE
                     INTARAERERMILEADKYSNEILSRAEGEADKLLNEAEAYKFRRISLAEGEAARIRSL
                     HAAYRDNPDLVLNNLWLESMEKVWPKLKVFIVDSGENSLQVLPLDEFFKSAGADAPGG
                     KQ"
BASE COUNT          482 a          843 c          901 g          678 t
ORIGIN      
        1 gttttgcctg agcacaggct gttctcgcgg cctgcgggaa agggctggga ctgtcgatgg
       61 aaaaggcggt ccgtatcgcc tccggtttcg cgagcggaat gcggacgggg gacacctgcg
      121 gcgccgtggc gggagccatt atggtcctgg gactccgcta cgggtccgaa gactgcgtca
      181 cggcggcagg gagagcggtt gtgtacggaa aagtggagga gttcaccagg cggtttcggg
      241 agcggaacgg atttcttctc tgccgtgatc tcctcgggct ggatacgagc accccggaag
      301 ggcttgcgaa ggcgaaggag ctgaatcttt tccggaccag gtgtcctgcc ctcgtggcgg
      361 atgccgcctc catactggaa gagatgataa aggaagaggg gtagttcccc gacaatgccg
      421 gggcagacca aagaaacccc ggaccggagg tgctgccggt ccggggtttc ttattcaccg
      481 ctgacggttc cggtctagaa gccaagctcc tccccgatga cgaacacgtg gaagggggtg
      541 gatctcgggc gcttgctgat gatggtcgtg acgttgcaga ttctcgtctg ggtctggcag
      601 tccatgcaga ggcccgtctg ggtgcacggg ttcggcaggc cgatcctctt gttgttgatg
      661 ggggcggcga tcatctggat gcgctcctcg gcggcatcga gatctgtcac gatcttgttc
      721 gtccccatca cgacgatgac ctttccgggg ccgaagatca tggcggcaac ccggttcccg
      781 ttcccgtccc tgttgacaag ccggccgtca agggtgacgg cgttcgtgcc ggagaggaac
      841 acgtcgcagg tgagctgctt cttcctgacg tcctgccgct cctcgttgga gaggccggga
      901 gcgccgtggt cgaggatcgt gtttcccttg ccggccagtt tctcctggat tccaagggcc
      961 tttaccgtcc aggaaccgcc gaagcccacc gacgccgatt caggaatcag ggccagtatc
     1021 ttgtccgcgg cttcggcggc ggaggagcag tattccgcct caaaaccgtt tttcttcagg
     1081 gattccacga ccttttttcc gagagcctcg ttgtgccagg gggtaaactc gttcattctt
     1141 cttcctcctc tttttggggc tgccccgggc gggcgccgtt tttcaactcg ttcctcccgg
     1201 gctgagttcc cacgcccttg aggtatttca ggatgccctc gcccgggcgg agcagtatcg
     1261 gggtgcccgg aagggcggtc tggtagaagt ccatggtctt gatgaacaga tacaggtcct
     1321 tcgcctccgc tgtagccttc ccaagggtat cctgggctat gctgtcgccc tgacccttga
     1381 tctcctctgc ttttcttctc gcctcggcga tcatttcaac ttctttcctg tcggcgaggg
     1441 accgccgggt gatgccctcc gcctgtccct cggctctgag ctggccggcc acccggtttc
     1501 gttcagcctg catggagcgg tagactgcct gctcgttgtc ctggggcagg aagacccgct
     1561 tataggcaac ggagaggatt tcaactccgt attccttcga ctgctccctg gcgatggccg
     1621 tggaactttc gatggcctgc tgccgcttgt ccttcaccag ctcgtccagg gtgagcctcc
     1681 cggacacgat gcggacggcc gagtatatgg aatcgtcgag acgctgctga acggagctca
     1741 cggtgcgcac tctccggaag aaggtttcgg gatcggagat cctgaagagg gcgaaggtgt
     1801 cgaacaccag gtttttcttg tcgctggtga ccaccgctac gggatcggcg tcgtattcca
     1861 cgagcttttt ggagtatctc gtgagctggt cgataaaggg gagcttgaag tacagtcccg
     1921 gttcggcccg ggtcgcgttc accttgccga gcctgaggac cacggcctgt tcatcggccc
     1981 ggaggatgta gaaggaaccg gagagcacaa ggaggacaag gagtaccgcc agggcgaata
     2041 ttttcttctt cattccggcc gcctcctatt gtttcccgcc gggagcgtct gctcccgcgg
     2101 acttgaaaaa ctcgtccagg ggaagcacct ggaggctgtt ttcccctgaa tccacaatga
     2161 agaccttcag tttgggccag accttttcca tgctttcgag ccagagattg ttgaggacaa
     2221 gatcggggtt gtccctgtag gcggcatgga gggagcggat tctcgccgct tcgccttcgg
     2281 caagggaaat ccgcctgaac ttgtaggcct cagcttcgtt gagaagcttg tcggcctcgc
     2341 cctccgccct ggacaggatc tcgttggagt acttgtccgc ttcgagaatc atccgttccc
     2401 tttcggcccg tgcggtgttg atttcactga aggctgcctg gaccgctttc ggagggttca
     2461 cgtcctggaa ctggacagcc acgatgaaca cgccggtctt caggaaatcc agcttttcct
     2521 ggatgagctt cttcgcctcc gtctgggagg cctctttttc cgtggtaagg acgtcgtcaa
     2581 gcttcctcga ggcgatgacc tcccggaggg tcgactccgc cacgtcccgg atcatttttt
     2641 ccctgtagga tctgtcttcc gggagattga ccacgtagga gaccggatcg cccgcctggt
     2701 actggagaac ccagtctatc tcaacgatct tgttgtcccc ggtgagcatc ttggattctt
     2761 ccggcctgtc ggaaaaggtc gcgggcggtc cctggttcac cgtcctgtac ccgtactcga
     2821 acctctggat ctgttccgtg ttcactatag tgacctggtc gatgacgggg agcttgaaat
     2881 tgatcccctg gtccgccact ccgg
//
LOCUS       DAOTHD010000172         2899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_40683_length_2899_cov_2.271800, whole genome shotgun sequence.
ACCESSION   DAOTHD010000172 DAOTHD010000000
VERSION     DAOTHD010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40683_length_2899_cov_2.271800"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="P5219_03990"
     CDS             complement(<1..994)
                     /locus_tag="P5219_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRX25948.1"
                     /translation="MVTFAHGRREIRLLARDPLLAFLVVLLFLSLALFVAYPLLSVLV
                     KSLQSDEGTFTFGNYTRFLTFKYLRSSLWNSLITGFATAAASVAVGYAAAFTITRTSI
                     RWKKALHLVFILPIISPPFTSALSILMLFGANGLITRGLLGIRNYNIYGFKGVLLSQI
                     FTFAPVAYLTLKGVLESLNPTLEDAAMNVGAGRMQTFLRVTLPLSLPGIASAFLVVLI
                     ESLADFGNPLVLAGSRFPMLSTQAYLEITGSFNLPLGAALAVVLLIPSITSFAIQRYY
                     LQKRQYTTVTGKPTASSSKLVSRGARRWLVTFAALFSGFVLLFYGTIFLGAFTKV"
     gene            complement(1062..2072)
                     /locus_tag="P5219_03995"
     CDS             complement(1062..2072)
                     /locus_tag="P5219_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRX25949.1"
                     /translation="MKKHGRLLTVLAGALLVLSLLAGAAASKELVVYSSVDEENAKNI
                     LDEFSKATGIRVNMVFLSSGPAMSRIEAEKGNPQADVWFGAPSENHIVAKNRGLTQSY
                     LPPSAADLAAGFKDPEGCWVAFYMNPLGFGALAEELKKDGKPVPASWKDLLDPAYKGM
                     IQMPSPQSSGTAYAMIMTLVETYGEDEAFKYMKALNPNIQTYTQSGTGPSKNLAIGET
                     QIAIQFTPAFLKLVDEGFPASVIFPAEGVGFEAAAMSILKGAKNIDSAKALADWIVSV
                     DGQKVLSAKKTYFFPVRADVSAGEGLPALSEIKLIDYDRIRAAQEKKRIIDRWVTEVL
                     GQ"
     gene            complement(2211..>2899)
                     /locus_tag="P5219_04000"
     CDS             complement(2211..>2899)
                     /locus_tag="P5219_04000"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRX25950.1"
                     /translation="CDPSMIRINLRELGIPLSLPASVLIASTADGLSTPRWTSPSGLS
                     YRLGNTWVTLIPSSTIEDLKNALQKEGHALGFHFSFGSPAADLMEISRSYQEARRTFS
                     LLRDFSLPPGVYFYEELSLYGLLQSMSKLPEARDVWERFWEPLEKDSNQARRSLPLKE
                     LASMLVARDFNAKSCADGLHLHYNTVRNYLREIETVLKVDLSNPHHRLGITLGCHING
                     SIAGQRSSLG"
BASE COUNT          562 a          785 c         1001 g          551 t
ORIGIN      
        1 agaccttcgt gaaggccccg aggaaaatgg tcccgtagaa gaggaggacg aagccggaga
       61 acagggccgc gaacgtgacg agccagcgcc gggccccccg gctgaccagc ttcgacgacg
      121 aggcggtggg ttttcccgtc accgtggtgt actgcctttt ctggaggtag tacctctgga
      181 tggcgaagga cgtgatggac gggatgagga gcaccacggc gagggccgct cccaggggaa
      241 ggttgaagga gccggtgatc tccaggtagg cctgggtgga gagcatgggg aagcggcttc
      301 cggcgagcac cagggggttg ccgaagtccg ccagggattc gatgagcacc acgagaaagg
      361 cgctggcgat gcccggaagg ctgaggggca gggtcaccct gaggaaggtc tgcatcctgc
      421 ctgctcctac gttcatggcg gcgtcctcca gggtggggtt cagcgattca agcaccccct
      481 tcagggtgag gtaggccacg ggggcgaagg tgaagatctg ggagagaagc actcccttga
      541 agccgtagat gttgtagttc cgtatgccca gcagtccccg ggtgatgagg ccgttggcgc
      601 cgaagagcat gaggatggag agggcgctcg tgaagggcgg ggagatgatg ggcaggatga
      661 agacaaggtg gagggctttc ttccaccgga tggacgtccg ggtgatggtg aaggccgccg
      721 cgtagcccac cgccacgctg gccgccgccg tggcgaaacc cgtgatgagg ctgttccata
      781 aggacgagcg gagatatttg aaggtgagaa agcgggtgta gttcccgaag gtgaaggtcc
      841 cttcgtcgct ctggaggctc ttcacgagca ccgagagcag gggataggcc acgaagaggg
      901 ccagggagag aaagagaagc actaccagaa acgcgagaag aggatcccgg gcgaggagcc
      961 ggatctccct ccgcccgtgt gcgaatgtga ccatgacgcc tcctgaaaag cggggggaag
     1021 gcgcagacct tccccccgcg ctgtctgtct tccgggagtt cctactgtcc caggacctcg
     1081 gtcacccagc ggtcgatgat gcgctttttc tcctgggccg ccctgatgcg gtcgtaatcg
     1141 atgagcttga tctccgaaag ggcgggaagc ccctctcctg cggagacgtc ggccctcacg
     1201 gggaagaagt aggtcttctt cgcgctcagc accttttggc cgtccactga cacgatccag
     1261 tcggcgaggg ccttggcgga gtcgatgttt ttcgctccct tgaggatgga catggcggcg
     1321 gcctcgaagc ccacaccctc ggccgggaag atgaccgagg cggggaagcc ctcgtcaacg
     1381 agcttcagaa aggccggtgt gaactgaatg gcgatctggg tctccccgat ggccaggttc
     1441 ttgctcggtc ccgtgccgct ctgggtgtag gtctggatgt tggggttgag ggctttcatg
     1501 tacttgaagg cctcgtcctc gccgtaggtt tcgacgagag tcatgatcat ggcgtaagcc
     1561 gtgcccgagg actgggggga gggcatctgg atcatgccct tgtatgccgg gtcgagaagg
     1621 tccttccatg atgccggcac gggcttgccg tccttcttca gctcctctgc cagggcgccg
     1681 aaaccgaggg ggttcatgta aaaggcgacc caacagccct cgggatcctt gaaacccgcc
     1741 gccaggtcag cggcggacgg gggaaggtat gactgggtca ggccccggtt tttcgccacg
     1801 atgtggtttt cgctgggggc gccgaaccac acgtcagcct gggggttccc cttttcagcc
     1861 tcgatgcggc tcatggccgg gccggaagag aggaagacca tgttcacccg gatgccggtg
     1921 gccttggaga attcgtcgag gatgttcttc gcgttctcct cgtccacgct ggagtaaacc
     1981 accagctcct tcgacgccgc cgccccggca agaagcgaca ggacgagaag cgcccccgca
     2041 agaacggtca acagtctgcc gtgtttcttc atttccggaa tccctccctg gaatgagtct
     2101 gtggagcata ggaattattg taacacattt cggtctccgg gaatattcgt ccctcctgtt
     2161 ttccggaaac gggaatggag ccttgatgtc cggcgacgcc gggaagccgc ttaccccagg
     2221 gaggagcgtt gcccggcgat gcttccgttg atgtggcagc ccagggtgat gccgagacga
     2281 tggtggggat tgctgaggtc cactttcagc accgtctcga tctccctgag gtagttcctc
     2341 acggtgttgt aatggaggtg gagcccgtcg gcgcatgact tggcgttgaa atccctggcc
     2401 acgagcatgg aagcaagctc cttcaggggc aggctccgcc gggcctggtt tgagtctttt
     2461 tccaggggtt cccagaatct ctcccagaca tcccgtgcct cgggcagctt cgacatgctc
     2521 tgcaggaggc cgtacaggga gagttcttcg taaaagtaga ccccgggggg aagggagaaa
     2581 tcccgaagca gggaaaaggt tctccgggcc tcctggtaag accgggaaat ttccatgagg
     2641 tccgcggcgg gagatccgaa agaaaaatga aaccccaggg catgcccttc cttttggagg
     2701 gcgttcttca ggtcctcgat ggtggaagag gggataaggg tgacccatgt gttgccgagc
     2761 ctgtagctca gtcctgacgg tgatgtccag cggggcgtgg aaagtccgtc ggcggtggag
     2821 gcgatgagaa ctgacgccgg aagggagagg ggaattccca gctccctgag attgatccgg
     2881 atcatggagg gatcgcagt
//
LOCUS       DAOTHD010000173         2897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_40714_length_2897_cov_2.915904, whole genome shotgun sequence.
ACCESSION   DAOTHD010000173 DAOTHD010000000
VERSION     DAOTHD010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40714_length_2897_cov_2.915904"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..266)
                     /gene="rpmC"
                     /locus_tag="P5219_04005"
     CDS             complement(51..266)
                     /gene="rpmC"
                     /locus_tag="P5219_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRX25951.1"
                     /translation="MEAKNLRDLTIEELNEKHRQYKEELFNLRFQNAIGQLQNTSRIK
                     AVKKTIARVLTVVREKQMAGEQSAGRR"
     gene            complement(269..691)
                     /gene="rplP"
                     /locus_tag="P5219_04010"
     CDS             complement(269..691)
                     /gene="rplP"
                     /locus_tag="P5219_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRX25952.1"
                     /translation="MLMPSRVKYRKPFRRPLKGRAKGGATVAFGEYGLQSLDCAWITA
                     RQIEATRVAISRKMKKGGKIWIRIFPDHPFTKKPLETRMGKGKGAPEFWVAPVKRGRI
                     LFEIAGVSKEVVEEAFRTASHKLPVKVRIVAREGFGGE"
     gene            complement(698..1366)
                     /gene="rpsC"
                     /locus_tag="P5219_04015"
     CDS             complement(698..1366)
                     /gene="rpsC"
                     /locus_tag="P5219_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRX25953.1"
                     /translation="MGQKVHPIGFRLGVVSEWESKWYAGGKDYAKNLHEDIALRKYIA
                     KKWEHAGISRIEIERVGHVIRFTVWTSRPGVVIGRGGAEIQVMKDDLQAKTGAKIMIN
                     VQEIKNPDVEAQLVAVGVASALERRVSFRRAMKQSIFRAMKGGAKGIKISTAGRLGGA
                     EIARTEWYNEGQLPLSTIRADIDYGFAEARTMYGVIGVKVWIFRDKANERPAQAPRQS
                     RKRG"
     gene            complement(1380..1712)
                     /gene="rplV"
                     /locus_tag="P5219_04020"
     CDS             complement(1380..1712)
                     /gene="rplV"
                     /locus_tag="P5219_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRX25954.1"
                     /translation="MEAKAVARQVRVSPIKVRRVLALIRGKKVGDALVALRFSTQKSA
                     KLVGKVLQSAVANAEHNFGMDTDKLRVVAATADQGPKMKRFRPVSMGRAHPYYHRTSH
                     VTVTVAER"
     gene            complement(1725..2006)
                     /gene="rpsS"
                     /locus_tag="P5219_04025"
     CDS             complement(1725..2006)
                     /gene="rpsS"
                     /locus_tag="P5219_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRX25955.1"
                     /translation="MARSRKKGPYVDQKLLRKVEDMNEGTAKKVLKTWSRRSTIVPPM
                     IGHTIAVHNGRIHIPIYISENMVGHKLGEFAPTRKFGGHAGQERSSRAR"
     gene            complement(2019..2822)
                     /gene="rplB"
                     /locus_tag="P5219_04030"
     CDS             complement(2019..2822)
                     /gene="rplB"
                     /locus_tag="P5219_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRX25956.1"
                     /translation="MSYKDFALGETRFKGLNKKDPERSLLEPLHKKGGRNNRGRITMR
                     HRGGGARRLYRVIDFKRNKLSVPGKIAAIEYDPNRNARIALVHYLDGEKRYILAPKDL
                     KVGDTIVAGPESDIRPGNALKLKDIPVGTMVHNLELEPGRGGKLVRAAGTSAQLMAKE
                     GKYAYLRMPSGELRLILQECMATIGQVGNEDYENVSLGKAGKTRWLGRRPMVRGMVMN
                     PVDHPMGGGEGRSKSHKHPVSPWGTPAKGYRTRKNKPSDRLIVRRRYEK"
BASE COUNT          483 a          902 c          822 g          690 t
ORIGIN      
        1 accgtaccgg tccggacctt gcggtgaggt ttccgctctt ccattcgggg ttacctcctt
       61 ccggcggact gctcgccggc catctgtttc tcacggacaa ccgtcagcac cctggcgatc
      121 gtcttcttca cagctttgat cctgcttgtg ttctggagct gcccgatggc gttctggaac
      181 cgcaggttga acagctcttc cttatattgt ctgtgcttct cgttcagttc ttcgatcgta
      241 agatcgcgca gattcttggc ttccatgttc attcaccacc aaaaccttct cgggcgacaa
      301 tccgcacctt gacaggcaac ttgtgagagg ctgtgcggaa ggcttcctct acgacctctt
      361 tcgaaacacc ggcgatctcg aaaagaatcc ggcccctctt cacgggagcg acccagaatt
      421 ccggagcgcc ctttcccttc cccatacggg tttcgagagg tttcttcgtg aaggggtgat
      481 cagggaagat acggatccag atcttgccgc ccttcttcat ctttctcgaa atggccacac
      541 gggtggcttc tatctggcgg gcggtgatcc aggcacagtc caggctctga aggccgtact
      601 caccgaaggc caccgtggcc ccgcccttgg cccgtccctt caggggacgg cggaaaggct
      661 tgcggtattt aactctgctc ggcatcagca tagcgtgtta cccccttttc cgggactgcc
      721 ggggcgcctg ggcgggacgc tcgtttgcct tgtccctgaa aatccagacc ttgactccga
      781 tgacaccgta catagtgcgg gcctcggcaa atccgtagtc gatgtcggca cggatggtgg
      841 aaagcggaag ctggccctcg ttgtaccatt ccgtccgggc gatttccgcg cctccgagac
      901 gtccggcggt ggaaatcttg attcccttgg ctccgccctt catggcgcgg aaaatggact
      961 gcttcatggc tctcctgaag gaaaccctcc gctcgagggc cgaagctacg cccactgcca
     1021 cgagctgagc ctccacgtca ggattcttga tctcctggac gttgatcatg atcttggcgc
     1081 cggttttggc ctgaaggtcg tctttcatga cctggatctc ggccccgccc cgtccgatca
     1141 ctacaccggg ccgggaggtc caaacggtga agcggatgac gtgaccgacc ctctctatct
     1201 cgatacggga aatgcccgca tgctcccatt tcttcgcgat gtacttccga agcgcgatgt
     1261 cctcgtgaag attcttcgca taatccttac cgccggcata ccacttggac tcccattcgc
     1321 tgacgactcc aagacggaat ccgattgggt gaactttctg tcccacgagt tccccctcct
     1381 taacgttctg ccactgtgac agtcacgtgg cttgtacggt ggtagtaggg gtgcgcccta
     1441 cccatcgaca cagggcggaa gcgcttcatc ttcggcccct gatccgccgt agctgctacg
     1501 acccgcagtt tgtccgtatc catgccgaaa ttgtgttcgg cgttggccac ggcgctctgc
     1561 aacactttac cgacgagctt ggcggatttc tgggtgctga acctgagcgc cacaagggca
     1621 tccccgactt tctttccccg aatgagagcc agcacccgac gaacctttat aggtgaaacc
     1681 cgtacctgcc tggcaacggc tttcgcttcc atcaccatca ctccttacct cgctcttgag
     1741 gagcgctcct gccccgcatg tccgccgaac ttgcgggtgg gggcaaattc cccaagtttg
     1801 tgaccaacca tattttcgct gatgtagatg gggatgtgga tcctgccgtt gtgcaccgcg
     1861 atcgtgtgtc cgatcatggg cggcacgatg gtggacctcc gggaccaggt cttgagcact
     1921 ttcttcgcgg tcccttcgtt catgtcctcc actttgcgga gcagcttctg gtccacatag
     1981 ggccccttct tcctggaacg agccatctta gtccctcctt acttctcgta acggcgccgg
     2041 acgataagtc tatcggacgg cttgttcttc cgggtgcggt atcccttcgc aggggttccc
     2101 cagggggaaa cgggatgctt gtgggacttg ctccgtccct cgccgcctcc catgggatgg
     2161 tctacagggt tcatgaccat accgcgaacc attggacgtc tgcccagcca acgggtcttc
     2221 ccggccttgc cgagggacac gttctcgtaa tcttcgtttc cgacctggcc gatggtggcc
     2281 atgcattcct gcaggatgag ccggagctca ccgctgggca tgcggaggta agcgtacttg
     2341 ccttctttcg ccatgagctg ggccgatgtg cccgcggcgc ggacgagctt gccgcccctg
     2401 ccgggctcga gctccaggtt gtggaccatg gtgcccacag ggatatcctt gagcttcagg
     2461 gcatttccgg gacggatgtc cgactcggga cccgcgacga tggtatcgcc gaccttaagg
     2521 tccttcggag ccagaatgta ccgtttttcg ccgtcaaggt aatggacgag ggcgatacgg
     2581 gcgttccggt tcgggtcgta ctcgatggcg gcgattttcc cggggacgga cagcttgttc
     2641 cttttgaagt cgataacccg gtagagcctg cgggcgccgc cgccgcgatg acgcatggtg
     2701 atcctgccgc ggttgttcct gccgcccttc ttgtgaaggg gctcgaggag tgagcgctcc
     2761 ggatccttct tgttgaggcc cttgaagcgg gtctcgccca gcgcgaagtc cttgtagctg
     2821 atggtcggag ccttgcagtc cagttgcagc ggattcttgc cctgctcgat caggcgcgga
     2881 tcgaagcggt acagcgg
//
LOCUS       DAOTHD010000174         2888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_40870_length_2888_cov_3.475821, whole genome shotgun sequence.
ACCESSION   DAOTHD010000174 DAOTHD010000000
VERSION     DAOTHD010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40870_length_2888_cov_3.475821"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="P5219_04035"
     CDS             complement(<1..936)
                     /locus_tag="P5219_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRX25957.1"
                     /translation="MDVIFDNLAIGFATAITATNILWVFIGGLLGTVMGMLPGLGPAT
                     GVAILIPITYGMNPSTALITMCAVYYGAMFGGSRASIMINTPGDASAIVSCFDGYPMT
                     KNGEAGAALAISAIASFIGGTIGMVFLVFLTGPIAAAALKFGPAEMFSLMLFALVATI
                     TLAEGSLLKGVIAMCIGFMLSTIGIDPLTGIIRFTFGVEELQDGIDFLVVMIGVYAIC
                     EVFKNYKNLDTKYSLDSRSIGKVWVSWAQFKKCILPMLRSSPLGFFVGVLPGMGASIA
                     TFMSYAMEKNLSKTPEKFGKGAIEGLAAPEAANNAA"
     gene            complement(950..1423)
                     /locus_tag="P5219_04040"
     CDS             complement(950..1423)
                     /locus_tag="P5219_04040"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRX25958.1"
                     /translation="MSKNFTSGLISIILGTIYFYTAMQFPEVQAGDNTGPKLFPLIVG
                     AVAVIAGILLCISDRRSGKAEAVAWNFLSDRAVWIKIFVTMVLGITYGLVLDTWGYLL
                     ATTAFMFFATMMINRGRFVQNVLIAVLFPAVTYGAFAIALQLSLPRGIIENMLPF"
     gene            complement(1512..>1794)
                     /locus_tag="P5219_04045"
     CDS             complement(1512..>1794)
                     /locus_tag="P5219_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRX25959.1"
                     /translation="AEILPGVPSFKEQGVDVVFPHWRGIIGAPDLTEDQVKYWDGVFS
                     KMVETETWKTLVKNLGWDSFYQNSAEHKAFLEGSTKDFDELLTQVGLKK"
     assembly_gap    1795..1804
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1805..2488)
                     /locus_tag="P5219_04050"
     CDS             complement(<1805..2488)
                     /locus_tag="P5219_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRX25960.1"
                     /translation="MKKLFAVLMLIALFATAGAASAEWKPSRTIELIAPANPGGGWDM
                     LCRTVQKVLTDEKLVEKNVIVVNKPGGGGATGWNYLKGKKGQGEYLAATSTLIMLNNL
                     LGKSELTHHDFTPLAALQTEWISVAVEQDSPWKTVKDLFEAIKANPSAVPVGVGPTLG
                     NNDHLVFLELAQANGVDPASIKFIVYPGAGGEIVPALLGGHVKATVIGLAEVLEQHKA
                     GKMRVIGVSS"
     gene            complement(2554..>2888)
                     /locus_tag="P5219_04055"
     CDS             complement(2554..>2888)
                     /locus_tag="P5219_04055"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRX25961.1"
                     /translation="TQYIGDELLERFFPIVKSFNHTVFEACRSTSLSAIIWAQREIFD
                     RYRVMRIILPNRIHKSLSRRKDLYAALRDGDPDRACAVWTDHLNESFTIWRDKSGYGK
                     ELEGFTFF"
BASE COUNT          559 a          789 c          950 g          580 t
ORIGIN      
        1 ggcggcgtta ttggcggcct cgggggcggc aagaccctcg atggctccct tgccgaactt
       61 ctcgggggtt ttgctcaggt tcttctccat ggcgtaggac atgaaggtgg cgatgctggc
      121 tcccattccc gggagcacgc ccacgaagaa cccgagggga gagctgcgga gcatgggcag
      181 aatgcatttt ttgaactggg cccagctcac ccagaccttg ccgatgctgc gggagtcgag
      241 gctgtatttg gtgtcgaggt ttttgtagtt cttgaagacc tcgcagatgg cgtagacgcc
      301 gatcatcacg acgaggaaat cgatgccgtc ctgcagttcc tccacgccga aggtgaagcg
      361 gatgatgccc gtgagggggt cgatgccgat ggtggaaagc atgaagccga tgcacatggc
      421 gatgacgccc ttgagcaggc ttccctcggc cagggtgatg gtggccacga gggcgaagag
      481 catgagggaa aacatttcgg cggggccgaa cttcagcgcc gcggcggcga tgggacccgt
      541 caggaagacg aggaagacca tgccgatggt gccgccgatg aaggaggcga tggcggagat
      601 ggccagggcc gctccggctt cgccgttttt cgtcatgggg tagccgtcga agcacgacac
      661 gatggccgac gcgtcgccgg gggtgttgat catgatggat gcccttgagc cgccgaacat
      721 ggcgccgtag tagacggcgc acatggtgat gagggccgtg gacgggttca tcccgtaggt
      781 gatgggaatg agaatggcca cccccgtggc gggcccgagg ccgggcagca tgcccatgac
      841 ggtgcccagc aggccgccga tgaagaccca cagaatgttg gtggccgtta tggccgtggc
      901 gaagcctatg gcgagattgt cgaaaatgac gtccatgatg ttcttcctcc tagaagggaa
      961 gcatgttctc tatgatcccc cggggcaggc tcagctggag ggctatggcg aaggctccgt
     1021 aagtgaccgc ggggaacaga acggcgatga ggacgttctg gacgaaccgc ccccggttga
     1081 tcatcatggt tgcgaagaac atgaaggccg ttgtggcgag caggtagccc caggtatcga
     1141 ggacgaggcc gtaggttatt cccaggacca tggtgacgaa aattttgatc cagacggccc
     1201 ggtcagacag gaaattccag gcgaccgcct ccgccttacc ggaacgcctg tcagaaatgc
     1261 acagcaggat gccggcgatg acggccacgg ccccgacgat cagggggaag agtttcggtc
     1321 ccgtgttgtc ccccgcctga acctcgggaa actgcatggc ggtgtagaaa tatatggttc
     1381 ccaggatgat ggaaatcagg ccgctggtga aatttttgct cattgagctc cctccgtctc
     1441 cagagatttc aaatgaaaag gggcggaaga ggccggagaa gggccccttc cgccggaatg
     1501 gtccggttcg gctacttctt caggccgacc tgggtaagga gttcgtcgaa atccttggtg
     1561 ctcccctcga ggaaggcctt gtgctcggcg gaattctggt agaagctgtc ccagccgagg
     1621 ttcttgacga gggttttcca cgtctcggtt tccaccatct tcgagaagac gccgtcccag
     1681 tatttcacct ggtcctcggt gaggtccggt gcgccgatga tgcctctcca gtgagggaat
     1741 accacgtcca ctccctgctc cttgaagctc ggaacaccgg gaaggatctc ggcannnnnn
     1801 nnnnggagct gacgccaatg accctcattt tgcccgcctt gtgctgctcg aggacctcgg
     1861 cgaggccgat gaccgtagcc ttcacgtggc cgccgagaag ggccggaacg atttctccgc
     1921 ccgcgccggg atagacgatg aacttgatgc tcgcgggatc cacgccgttg gcctgggcga
     1981 gttcgaggaa gacgaggtgg tcattgttgc cgagggtggg gcctacgccc acgggcacgg
     2041 cggagggatt cgccttgatg gcctcgaaga ggtccttcac ggtcttccag ggagaatcct
     2101 gctccacggc cacggaaatc cattcggtct gcagggcggc caggggagtg aagtcgtgat
     2161 gggtcagctc gctcttgccg aggaggttgt tgagcatgat gagggtggac gtggcggcga
     2221 ggtactcgcc ctgacccttc tttcccttca gatagttcca tcctgtggcg ccgccgccgc
     2281 cgggcttgtt gaccacgatg acgttcttct ccaccagctt ctcgtcggtc aggacctttt
     2341 gcacggtgcg gcagagcatg tcccatcccc cgccggggtt ggccggggcg atgagctcga
     2401 tggtccggga tggcttccat tccgccgaag ccgcccccgc ggttgcgaac agcgcgatga
     2461 gcataagaac tgcgaacaac tttttcattc ctctgtttcc ccctttaaaa tatgatgatg
     2521 cgtctccggg cactgccgtg cgcgggaaaa gccctaaaag aaggtgaagc cttcaagttc
     2581 cttaccgtac cccgacttgt ccctccagat ggtgaaggat tcgttcaggt gatccgtcca
     2641 gacggcacag gcccggtcag ggtcgccgtc ccggagggcg gcgtacaggt cttttctgcg
     2701 ggaaagactt ttgtggatcc tgttgggcag gatgatcctc atgacacggt agcggtcgaa
     2761 aatctcccgc tgggcccaga tgatggctga gaggctggtg gatctgcagg cctcgaagac
     2821 ggtatggttg aaactcttca ctatggggaa aaaacgttcg agaagctcgt cgccgatgta
     2881 ttgggtaa
//
LOCUS       DAOTHD010000175         2878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_41091_length_2878_cov_4.043571, whole genome shotgun sequence.
ACCESSION   DAOTHD010000175 DAOTHD010000000
VERSION     DAOTHD010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41091_length_2878_cov_4.043571"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="P5219_04060"
     CDS             complement(<1..307)
                     /locus_tag="P5219_04060"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRX25962.1"
                     /translation="MKKIRRILPAVSLLALLFTCAAEAAIDQAAVHRAWAKISAKSGI
                     EHKPVNFENKKEPNAWVKFSPGNHSVHVTTGLMAILDEEDEIAGILARRCVNYCQPGD
                     "
     gene            596..1594
                     /locus_tag="P5219_04065"
     CDS             596..1594
                     /locus_tag="P5219_04065"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRX25963.1"
                     /translation="MWRYASSLPLEKPEEKVSFGEGWTPLVREEWNGMNLFLKLDYAC
                     PTGSYKDRGMAYLVSRLRDQGISEVIEDSSGNAGASMAAYCARAGIRCRVFVPAYTSE
                     GKCVQVASYGADLERVPGSREDTTRAAEKAAETVFYGSHNWSPWFAHGIKTFAFELWE
                     QLGFTAPEAVLLPAGQGSLVLGCALAFEELLSSGEIETLPKIFALQPKNCAPLEEAFR
                     KGSPSPERVEKRETIAEGISSAEPVRGRDVLAAVRKTGGAVLSFSDREIWDGFVKLAR
                     RGYFVEPTSAIVGAAAEMLSRDGSVGGKRPVAAVLTGSGLKGSGKILSLFSSPPED"
     gene            1636..2874
                     /locus_tag="P5219_04070"
     CDS             1636..2874
                     /locus_tag="P5219_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRX25964.1"
                     /translation="MGRVSVNNLREGMVLEEDLSAPNGRFILGRGSVLKENHIRMFKI
                     WGVTGASVEGAEDAEIALEEHLREEALRSAGTFVDSYLPPAEGETPASAGIRSFCTRK
                     FAGEIASGKLPAPLPEMKQLPRPPEGHFGRTFSAYGLAKNEVRLVSFPDIYFKIQEVI
                     NSPISSATTIAKVVSKDPSLTARLLRLVNSSFYGFPNPILSIPRAIAIIGANELTALA
                     LAVSTMTVFRHVPPAYVDMKSLWKHSIACGVFSRLLAYSRRIRNEERFFLAGLLHDLG
                     RIILYTKTPAEMTYALELSFFERIPLWRAEKRVFGFDHAAVGKVLLEEWNIPESIRKL
                     CDFHHSPLDENAPEESAFVHAADYIANGLRAGTSGSVYISAVAPEIWDYLGLDEGDLE
                     SVLVQGERQIREIMHIFLVA"
BASE COUNT          607 a          788 c          830 g          653 t
ORIGIN      
        1 ggtcgccggg ttggcagtaa ttgacgcagc gccgggcgag gatgccggcg atttcgtcct
       61 cctcgtcaag tatggccatc agtccggtcg ttacatgaac cgagtgattc cccggtgaaa
      121 acttcaccca ggcgttgggt tcctttttgt tctcgaagtt gacaggcttg tgttcgattc
      181 ctgactttgc ggaaattttt gcccatgcac ggtgcaccgc ggcctggtct atggcggctt
      241 cagcagcaca cgtgaaaaga agggccagaa gagaaacggc gggaaggatt ctccgtattt
      301 ttttcacaac gttctccccc ttttttccgg ggaaataccc cggaggaatg tctttcaatt
      361 acagcgtttt cattgaaatt cttgcgtatt ttttccctgc aggaaagggg aaaattacgt
      421 ataatatcct attatgacca atcacggaaa aataatgctt caatgctccc tctgcggtac
      481 ggtggtccgg gaagacagtc ttctgtggag gtgtgtgtgc ggggctccgc ttcttgcgga
      541 cttcagcgga actcctccta tggacagaag ccgaatatgt cccgctgagc agggaatgtg
      601 gcgctatgca tcgtcccttc ccctggaaaa accggaggag aaagtgtcct ttggtgaggg
      661 gtggacccct cttgtccggg aagaatggaa cggcatgaac ctcttcctca agctcgacta
      721 tgcctgccct accggctcct acaaggaccg ggggatggca tacctggtaa gcagactgag
      781 ggaccaggga atctccgaag tcatcgagga ttcttcagga aacgccggag cctccatggc
      841 ggcttactgt gccagggctg gcatccggtg ccgggttttt gttcccgcct acacctcgga
      901 ggggaagtgt gtccaggttg cctcctacgg cgcggatctt gagcgggtgc ccggttccag
      961 ggaggatacg accagggcag cagagaaggc tgcggagacc gtattctatg ggagccacaa
     1021 ctggagtccg tggtttgccc acgggatcaa gaccttcgca ttcgaactgt gggagcagct
     1081 tggattcacc gctccggaag ctgttcttct tcctgccgga cagggaagcc tcgttctcgg
     1141 ctgcgccctg gcctttgaag aacttctttc aagcggagaa atagaaaccc ttccgaagat
     1201 attcgccctt cagccgaaaa actgcgctcc cctggaggag gccttcagga aagggagtcc
     1261 ttctccggag agagtggaaa agagggaaac catagcagaa ggtataagct ccgcggaacc
     1321 tgttcgaggg cgagacgtcc tcgctgcggt tcgaaaaacc ggaggagccg tcctttcttt
     1381 ttcggacagg gaaatctggg acggatttgt gaagctcgcc cgaagaggat acttcgttga
     1441 acccacgagt gccattgtcg gcgctgcggc tgaaatgctt tcccgtgacg gttcggtagg
     1501 cgggaaacgc ccggtggcgg cggtccttac cgggtcgggt ctcaagggaa gcggcaagat
     1561 cctctccctg ttttcctctc ccccggagga ctgaatgaaa cgacggcata ttccttggca
     1621 ggaaacggac ctttcatggg cagggtgagc gtaaacaatc tccgggaggg gatggtcctc
     1681 gaagaagacc tgtcggctcc aaacgggagg ttcatcctgg gcagaggctc tgttctgaaa
     1741 gagaaccata tccggatgtt caagatatgg ggcgtcaccg gtgcgtcagt ggaaggagcc
     1801 gaggatgcgg aaatagcgct ggaggagcac ctccgggaag aggctctcag gtcggccggg
     1861 acttttgtgg attcctacct cccgcctgcg gaaggcgaaa cgccggcttc agcgggaatc
     1921 cggtcattct gcacgagaaa attcgccgga gaaatcgctt cgggcaaact tccggcacct
     1981 cttcccgaaa tgaaacagct cccgcgccct cccgagggac acttcgggcg gactttttca
     2041 gcttatggcc tcgcaaagaa cgaagtacgg cttgtttcat tcccggatat ctacttcaag
     2101 atacaggaag tcatcaattc tccgatcagt tcagccacca ccatcgctaa agtggtgagc
     2161 aaggatccca gtcttaccgc ccgccttctt cgtctggtga acagctcctt ttacggtttc
     2221 cctaacccga ttctgtccat tcccagggcc atcgccatca tcggggcgaa cgaactgacg
     2281 gccctcgccc ttgcggtttc caccatgacg gtgttccgcc atgtgccgcc cgcatacgtt
     2341 gacatgaaat ccctctggaa gcactccatc gcatgcggtg tcttttcccg cctattggcc
     2401 tattcgagga ggatccggaa cgaggagcgt ttttttctcg ccggactcct gcacgacctc
     2461 ggaagaatca ttctttacac caagacgccg gcggagatga cctatgccct cgagctttcc
     2521 ttttttgagc ggattcccct ctggcgcgcg gaaaagaggg ttttcggctt cgaccacgcc
     2581 gctgtgggaa aggttcttct cgaagagtgg aacatccctg aaagcatcag gaagctctgc
     2641 gacttccacc attcgccact ggatgaaaac gctccggagg aatccgcctt tgttcatgcc
     2701 gcggattaca tcgccaacgg actccgcgcg ggaaccagcg gatcggtcta tatttccgcc
     2761 gtcgcgccgg aaatatggga ctatctcggg ctcgacgaag gggatcttga atcggttctt
     2821 gtccagggcg aacggcagat ccgggagatc atgcacattt ttcttgttgc ctgaggct
//
LOCUS       DAOTHD010000176         2872 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_41206_length_2872_cov_2.990415, whole genome shotgun sequence.
ACCESSION   DAOTHD010000176 DAOTHD010000000
VERSION     DAOTHD010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41206_length_2872_cov_2.990415"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..927
                     /gene="cobC"
                     /locus_tag="P5219_04075"
     CDS             277..927
                     /gene="cobC"
                     /locus_tag="P5219_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ribazole phosphatase"
                     /protein_id="HRX25965.1"
                     /translation="MPKKRLLLVRHGKTEWNGLSRFQGKSDVPLNDEGKCQAELLSAR
                     LEGWDPDALFSSPLSRARETAETLAFRNPGISPVLLEELSEIGFGSWEGRSIHEIESA
                     DPESFRRWKDSPFDYPPPGGESFASVESRVRSALGEILSKSGERIVVVSHGGILRAML
                     ALLLDLPLRPVWKMKIANCSVTGIDVGRKGTSLAFLNDDLHARMSGAEPEKLLFPV"
     gene            989..1675
                     /locus_tag="P5219_04080"
     CDS             989..1675
                     /locus_tag="P5219_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRX25966.1"
                     /translation="MSDREQDAHAAGSLLFPGKQGENSSTHPGRTEPRDHSVNERTLS
                     PEREEELWRRLSSSGDEDARDEIIVAYRPLVFWLARKFRVRPSSYQDLIQEGMVALIR
                     AVDKFEPERHLRFTTYAFYRIKGQMVNFLQRSELKAPVPVDDEYLMPEDSFAPDSFET
                     LIAVSEEMHRLPAKEEEIVKALLVEGQDAKDVARKRGIDISHVYRLKRNGVAKLRKWL
                     GPEGDATNRA"
     gene            1729..2334
                     /locus_tag="P5219_04085"
     CDS             1729..2334
                     /locus_tag="P5219_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25967.1"
                     /translation="MEKKIARIARWLDRCSKACSAMSWQSALLDMECAKAELEEARAQ
                     LWARAEGSTQASPVVRRAGRMALAAVLGLFLVLSVAGPVAVQTAREAVSIASGPSIEW
                     VSTDEKALLSALRRSLSDANLARLSAEPEESGPAGKAETTTVARQTDSSRNSMARQRG
                     PKEKLQNPSSSGREFDTIITLVQIGQKALRERESAIRFDAP"
     gene            2372..>2872
                     /locus_tag="P5219_04090"
     CDS             2372..>2872
                     /locus_tag="P5219_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HRX25968.1"
                     /translation="MNVDLKKSWRILVLLFAVILLPNAAFAAEPLVSALAVEGNQQVV
                     AEHILGVVGTRVGEPLSRVQLQTDVEAIYNLGFFSFVDISLQSVAGGAAVTYVVTENP
                     VVEQITFTGNNIYTDEDLLKVVFTSPGTVFNRVFFRNDLDRIQEKYHKDGYVMVKIAD
                     VQIAMAF"
BASE COUNT          624 a          808 c          846 g          594 t
ORIGIN      
        1 gagcagcgca ctcgtaatgc gcaggtcggc agttcaagtc tgcccgccgg ctccagtaac
       61 agcaagggtt cccgtggaaa tcgggagccc ttttttgttg cctgtatctc acccgtgtgt
      121 cccagcctcc acatagcctc cagtatccat tcctccggaa aatacctcct caaatccgcg
      181 gcgtctcaaa gtgtcgctca atgaaaagaa acctgcggca aggctctttt catgctatca
      241 tcgtcgtatc aaaatgttcg ctcttcggag gctattgtgc ccaaaaaaag acttctcctc
      301 gtgcgccacg gaaagacgga atggaacgga ctctcccgtt ttcagggaaa aagcgatgtc
      361 cccctcaacg atgaaggaaa atgccaggct gagctgctgt cggcaaggct ggaaggctgg
      421 gatcctgacg ccctgttttc gagccccctg tcgagggccc gggaaaccgc ggagactctt
      481 gctttccgca accccgggat ctctccggtt ctcctggagg aattgtccga gatagggttc
      541 gggagctggg aaggccgttc catccatgag atcgagtccg ccgatcccga atccttccgc
      601 agatggaagg attccccctt cgattatccc cctcccggag gcgaatcctt cgcttccgtg
      661 gaatcccgtg tgaggtcggc cttgggcgaa atcctgtcaa agagcgggga aaggatagtg
      721 gtggtgagcc acggaggaat cctcagggcc atgctggccc ttctcctgga tcttcccctc
      781 cggccggtct ggaaaatgaa gatcgccaac tgctcggtca ccggaatcga tgtggggagg
      841 aagggaacgt cccttgcctt tctcaacgac gatctgcacg ccaggatgtc gggggcggag
      901 ccggaaaagc tgctttttcc ggtttagact tctttttctc ttgctggtac aattaaatgc
      961 gttgccgagc aaaggaggcg catgagccat gtctgaccgg gagcaggatg cgcacgccgc
     1021 aggttccctt ctcttccccg gaaagcaggg agagaatagc agtacccatc cgggacgaac
     1081 ggagccccgg gatcattccg taaatgaaag aaccctctcg cccgagaggg aagaggaatt
     1141 gtggcggagg ctctcctcct ccggcgacga ggacgccagg gatgaaatca ttgttgctta
     1201 ccgtcccctt gtattttggc tggcacggaa attccgcgtc cggccgtcct cttaccagga
     1261 tctcatccag gagggaatgg tggcgctgat cagggcagtg gacaagttcg agcccgagcg
     1321 tcacctccgg ttcaccacct acgccttcta ccggatcaag ggacagatgg tgaatttcct
     1381 ccagaggagc gaactcaagg ctcccgttcc cgtggacgac gagtacctca tgcccgagga
     1441 ttccttcgcc cctgattcct ttgaaacact catcgccgtg tccgaggaaa tgcaccgcct
     1501 tccagcgaag gaggaagaga tagtcaaggc tctcctcgtg gaagggcagg acgccaagga
     1561 tgtggccagg aaacggggca tcgacatcag ccatgtctac cggctcaagc ggaacggggt
     1621 ggcgaagctg cgcaagtggc tcggcccgga aggagacgcc acaaacaggg cctgatacgg
     1681 gataagagtt atgcggcaca gacttttatc tcccgggggg actgccttat ggaaaaaaag
     1741 atcgcccgaa tagcccggtg gctcgacagg tgttccaaag catgttccgc catgtcgtgg
     1801 cagagcgcat tgcttgacat ggaatgcgcg aaggccgagc ttgaggaagc ccgggcgcag
     1861 ctgtgggcca gggctgaggg cagcacccag gcctctcccg tggtgcgcag ggcgggacgg
     1921 atggcactgg cggcggtcct gggactgttt ctcgtccttt cagtagccgg tcccgtggcg
     1981 gtccagacgg caagagaagc tgtatccatt gcttccggac catccatcga atgggtttcc
     2041 accgatgaaa aggcccttct ttcggccctc cgtcggagcc tgagcgacgc caacctggcc
     2101 cgtctctctg cggaaccgga agaaagtggc ccggccggga aagctgagac aaccactgtc
     2161 gcccggcaga ctgattctag tcggaattcg atggcccgcc agagggggcc aaaagaaaaa
     2221 ttacagaatc cctcctcttc cggcagggaa tttgatacaa taatcactct ggtacagatc
     2281 gggcaaaagg ctcttaggga aagggagtcc gcgattcgtt tcgatgcccc gtaacgaagt
     2341 tttttccatt ttccggaagg agagatcgtg cgtgaatgta gatctgaaaa aatcgtggcg
     2401 gatcctggtg ctcctctttg ctgtaatcct gttgccgaat gccgcctttg ccgcggaacc
     2461 ccttgtttct gcccttgccg tggaaggaaa ccagcaggtt gtggcggagc acattcttgg
     2521 ggtcgtgggg accagggtgg gtgaacccct cagcagagta cagcttcaga cggatgtgga
     2581 ggccatatat aacctcgggt tcttttcctt tgtggacatc agcctgcaga gtgtcgcagg
     2641 aggagcggct gtgacctacg tcgtcacgga gaaccccgtg gtggaacaga tcaccttcac
     2701 cggcaacaac atctacactg acgaggacct tctgaaagtc gtattcactt cgccgggcac
     2761 tgtgttcaac agggtcttct tccggaacga cctcgaccgg atccaggaga agtaccacaa
     2821 ggacggctac gtcatggtaa agattgccga cgtgcagatc gccatggcat tc
//
LOCUS       DAOTHD010000177         2871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_41224_length_2871_cov_3.040838, whole genome shotgun sequence.
ACCESSION   DAOTHD010000177 DAOTHD010000000
VERSION     DAOTHD010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41224_length_2871_cov_3.040838"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="P5219_04095"
     CDS             <1..1131
                     /locus_tag="P5219_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRX25969.1"
                     /translation="SSMRGITTAILESKVPVVVWVSPSGARAASAGAFILQAAHVAAM
                     APGTNAGAAQPVMASGKDAPDTDMKKKITNDLAAQIRSLAQLKKRNADVAEKMVTESL
                     SITAEEALSLGVVDLVASDMESLLLGLRGREVTAGSRTMHLDPPSGPPVEVSMSLREK
                     ILQFVSSPDIAYLLLTAGVLAIIFEVMSPGGFVLGTAGAVMVLLGAFGLRMLPFNWAG
                     IILLAAGVGVLVLDLLVGGIGVLSLFGLAALVTGGLILFRAPGGELLNVSMSFIAGMS
                     IALGLFFFAALYLVLRSLKRRTSSGREGMIGMEAEVMEDLAPVGLVKCRGEIWRARSA
                     DGAPLAKGRTGRVAAVRGMTLVIEEDGGKVLPAGGSSEKSAG"
     gene            1146..1937
                     /locus_tag="P5219_04100"
     CDS             1146..1937
                     /locus_tag="P5219_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HRX25970.1"
                     /translation="MIIDAVEMILSIGTSFGFLLIAVFILASAVKVVPEYQRVVVFRL
                     GRLVGSKGPGIVLVIPVIDRVVRVDLRVVTLDVPVQEVITRDNVPIKVNAVVYFRVMD
                     PAFSVVEVENYILATSLLSQTTLRSVVGSADLDEVLSSREKINSELQKIIDERTDSWG
                     IKVSAVEVKELELPESMKRAMAKQAEAERERRAKIINAEGEMQAAQKLSEAAKQMETS
                     PITLQLRYLQTLREISGDKNSTTIFPLPLDLVKPFMDRFFKKDEA"
     gene            2163..>2871
                     /locus_tag="P5219_04105"
     CDS             2163..>2871
                     /locus_tag="P5219_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRX25971.1"
                     /translation="MGYDFQSIEKKWQHTWESEGTFNADAEPGREKFYCLEMFPYPSG
                     ALHMGHLRNYSIGDMLTRFLWKKGLNVLHPIGFDAFGMPAENAAMKYNTPAAEWTWRN
                     IEHMTEQLKHMGCSYDWRRRVETCNPDYYRWTQWIFLQFFKKGLAYRKNAPVNWCSSC
                     STVLANEQVINDGHCWRCGTPVVKRNLEQWFLRITDYAQELLDDLDNLPGWPERVKIM
                     QRNWIGRSEGARLSFTEK"
BASE COUNT          604 a          785 c          916 g          566 t
ORIGIN      
        1 agttccatga gggggatcac cacggcgata ctcgaatcga aggtaccggt ggtggtgtgg
       61 gtttcaccga gcggtgcgag agcggcatcc gcaggagcct tcatcctcca ggcggcccac
      121 gtggcggcca tggcgccggg aaccaacgcg ggcgcggccc agccggtaat ggcgtcgggg
      181 aaggacgcgc ccgacacgga catgaaaaag aagataacga acgacctcgc cgctcagatc
      241 agatcccttg cccagctgaa aaagcggaac gcggacgtgg cggagaagat ggttaccgag
      301 agcctctcca ttacggctga ggaggccctt tccctcggag tggtggatct cgtggcctca
      361 gatatggaat ccctcctcct cggccttcgg ggaagggagg tcacggctgg cagccggacg
      421 atgcacctcg atcctccttc cgggccgccg gtcgaggtgt ccatgagcct cagggagaaa
      481 attctccagt ttgtctccag tcccgacatt gcctatcttc tccttaccgc gggggtgctc
      541 gccatcatct ttgaggtcat gtctcccgga ggattcgtgc tcggaactgc cggggccgtc
      601 atggtccttc tcggggcatt cggcctcagg atgctgcctt tcaactgggc gggcatcatc
      661 cttctcgccg caggcgtggg cgtcctggtc ctcgacctgc tggtgggcgg aatcggcgtg
      721 ctcagcctgt tcggcctcgc ggccctcgtg accggcgggc tcatactctt ccgtgctccg
      781 gggggtgagc ttttgaacgt gtccatgagc ttcatcgcag gaatgtctat cgccctgggg
      841 ctgttctttt tcgccgccct ctatcttgtc ctgcggtctc tgaaacgcag aacctcctcc
      901 ggcagggagg ggatgatcgg catggaggct gaagtgatgg aggacctggc acccgtgggg
      961 ctcgtcaagt gcaggggaga gatctggagg gcaaggagcg ctgacggtgc gccccttgca
     1021 aagggaagga cgggaagagt ggcggcagtc cgggggatga ccctggttat cgaagaagac
     1081 ggcgggaagg ttcttccggc cgggggaagt tcggaaaaat cagcaggtta aaaggaggag
     1141 aacccatgat tattgacgca gtggagatga ttctcagcat aggcacgtcc ttcggatttc
     1201 ttctcatcgc cgtgttcatc cttgcatcgg cggtgaaggt ggtgccggag taccagcggg
     1261 tggtggtctt ccgtctcggg aggctcgtgg gttccaaggg acccggcatc gtcctggtca
     1321 tcccagtcat tgacagggtg gtcagggtag acctgcgcgt cgtgaccctg gatgttcccg
     1381 tccaggaggt tatcacgagg gacaacgttc ccatcaaggt aaatgcggtg gtgtatttcc
     1441 gggtcatgga cccggctttc tctgtggtgg aagtggaaaa ctacatcctg gcaacgagcc
     1501 ttctctccca gacgaccctc cgttccgtgg tggggtcggc cgaccttgac gaggtcctgt
     1561 cttcgaggga gaagatcaac agcgagcttc agaagatcat cgatgagcgg accgattcct
     1621 ggggcatcaa ggtcagtgcc gtggaagtga aggagctcga gctgcctgaa agcatgaagc
     1681 gggccatggc gaaacaggcg gaggcggagc gggagcggcg ggcgaagatc atcaatgccg
     1741 agggagaaat gcaggccgcc cagaagctca gcgaggcagc gaaacagatg gagacgtccc
     1801 ccatcactct gcagctgagg taccttcaga cgcttcggga gatcagcggg gacaagaact
     1861 caaccaccat attccccctg cccctcgacc ttgtgaaacc tttcatggac agatttttca
     1921 agaaggatga agcttagggc aggaagaact ttttcgggat gaaggccgtt ccgggtggat
     1981 ttttctcccc cggaacggct tcctttggta tataatgccg gaaaaggccg gttcgttccg
     2041 aagaggacga acagcccaac gaccgacgtc cgggctgacg gtttccccgc cagtcgcaca
     2101 gggcggctgg tcgtttgcgc gtttgcagat aaaatgcagt gttcatcaag gaggaagggt
     2161 caatgggcta cgattttcaa tccatcgaga aaaaatggca gcatacatgg gagagcgaag
     2221 gcacgttcaa cgccgatgcg gaacccggca gggagaagtt ctactgcctg gaaatgttcc
     2281 cctacccgag cggcgccctc cacatggggc acctgaggaa ctactctatc ggcgacatgc
     2341 tgacccgttt cctctggaag aagggactga acgttcttca tcccatcggt ttcgatgctt
     2401 tcggcatgcc tgcggaaaac gccgccatga aatacaacac ccctgcggcg gagtggacct
     2461 ggcggaacat cgagcacatg acggagcagc tgaagcacat gggatgcagc tacgactggc
     2521 gcagacgggt cgagacatgc aatcccgact attaccggtg gacccagtgg attttcctcc
     2581 agtttttcaa aaaagggctt gcctacagga aaaacgctcc ggtgaactgg tgttcaagct
     2641 gcagcaccgt gctcgccaat gagcaggtca tcaacgacgg gcactgctgg agatgcggta
     2701 cgccggtggt gaagcgcaac ctcgagcaat ggttcctgag aataaccgac tatgcccagg
     2761 aactccttga cgatctcgac aaccttcccg gatggccgga acgggtcaag atcatgcaga
     2821 ggaactggat cggccgttcg gagggtgccc ggctctcctt cacagagaag a
//
LOCUS       DAOTHD010000178         2864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_41375_length_2864_cov_2.851549, whole genome shotgun sequence.
ACCESSION   DAOTHD010000178 DAOTHD010000000
VERSION     DAOTHD010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41375_length_2864_cov_2.851549"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="P5219_04110"
     CDS             <1..389
                     /locus_tag="P5219_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRX25972.1"
                     /translation="PEQALEHVLGYTCANDVTARDLQSRDGQWTRSKSFDTFCPLGPW
                     IVSGIDPSDLEIGMYLNGKQVQHSRTKNFIFPVDVLVSHISKVMTLLPGDVIITGTPS
                     GIGPVKKGDVMQVKIEGIGTLENVVE"
     gene            complement(501..586)
                     /locus_tag="P5219_04115"
     tRNA            complement(501..586)
                     /locus_tag="P5219_04115"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(550..552),aa:Ser,seq:tga)
     gene            711..1124
                     /locus_tag="P5219_04120"
     CDS             711..1124
                     /locus_tag="P5219_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRX25973.1"
                     /translation="MKKEEILERFVCTPSRVAVVGASPKEDRPVFRVMNYLAGAGFTL
                     YPVNPGYAGTEIMGRPCAAGLEFLEDEVDVVALFLSAKQQGTVAADLEKLPVRPVVWF
                     QPGAENEELAAELERKGYDVVSSDCLMASHMNKCR"
     gene            1294..1704
                     /locus_tag="P5219_04125"
     CDS             1294..1704
                     /locus_tag="P5219_04125"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRX25974.1"
                     /translation="MSSEYPYTLRVRYGDTDQMGVAYYANYLYWFEVGRTEFCRAHGK
                     SYADWEEEGIFLPVVESHCRYKHPARYEDEIVIFTKIGEVKASSVIFECRIERAADGK
                     PLASGWTRHAFVDRNGRLLRGDNPLRRWIEALAG"
     gene            1701..>2864
                     /locus_tag="P5219_04130"
     CDS             1701..>2864
                     /locus_tag="P5219_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HRX25975.1"
                     /translation="MTEVSRTVSTKAILLGNEAIARGIVEAGCLVASAYPGTPSSEIL
                     PAVAKFADELGSPMAVEWGVNEKVAFEVAAAASFTGARSCAVMKQVGLNVAADPFMSA
                     AHFQLKGGMLLVVADDPGPHSSQTEQDSRYFAMFAKVPCFDPCSAAEAKEMVFDAFDL
                     SERFGIITMLRPSVRVDHCRQDVELGELRPPKGQVKFEKEPSRWVCLPASVKVNHPRL
                     NAKVEEIRAAFETEFGKYNYEVPSRGKSVLGIVASGVCFSVVSDILKEWGREDVAVLK
                     IGTPHPLPVKMVTEFISRHPKVLFLEETYPVMEMQLPDRTGILGRWNGVVPGAGELLP
                     EVIAGILAKILGMDQPSGEGSSLLREALEDLKITPRKPMLCPGCPHRASYFSIR"
BASE COUNT          583 a          829 c          900 g          552 t
ORIGIN      
        1 ggccggaaca ggcgctggag catgtactgg gctatacctg cgccaacgat gtcacggccc
       61 gggatctgca gtccagggac ggccagtgga cccggtccaa gtccttcgac accttctgcc
      121 ccctggggcc gtggatcgtc tcgggtatcg atccttcgga cctcgagatc ggcatgtacc
      181 tgaacgggaa acaggtccag cattccagaa caaagaactt catcttcccc gtggacgtgc
      241 tggtgagcca catctcgaaa gtcatgaccc tcctgcccgg agacgtgatc atcaccggca
      301 ctccgtcagg catcggcccc gtgaaaaagg gagacgtcat gcaggtgaag atcgaaggga
      361 tcgggaccct ggaaaacgtg gtggagtagc ggggggcgaa acaaccttcc gccgctgacc
      421 ctgtgctgca aaaaaacgtc cccgccggtc agccggcggg gacgtttttc attcggtgaa
      481 gcttccgttt agcggaaaaa tggcggaggg cagaggattc gaacccctgg ggctttcgcc
      541 cagctgattt caagtcagcc accatagacc actcggacag ccctccgcgg aacgaggaga
      601 ttatacatgt atttcccgtt gttgccaacg gatgaagggg ggcgcccgca aattcggggg
      661 agagtataat gaatggaagt ggaccgattt ttcctgagag gtgattcctg gtgaaaaaag
      721 aagagattct cgagaggttc gtgtgcaccc cgtcccgggt ggcggtggtg ggagcgtcgc
      781 cgaaggagga ccgcccggtg ttccgggtga tgaactacct tgccggtgcg gggttcaccc
      841 tctatcccgt gaaccccggg tatgcgggaa cggagatcat gggacgtccc tgcgccgcgg
      901 ggctcgaatt tctcgaagat gaggtcgacg tggtcgccct gttcctttcg gcgaaacagc
      961 aggggactgt ggcggcagac ctggagaagc tgcccgtcag gcccgtggtc tggttccagc
     1021 cgggagcgga gaacgaagaa ctcgccgctg aactggagcg gaaaggatac gacgtggtgt
     1081 cgtcggactg tctcatggcc tctcacatga acaaatgcag gtgagaatta attcttgctc
     1141 ctgacgccat cggaatgata aaaatttctt gcctcccggt gcgaatttat atataattac
     1201 agaaaagacc gcacttgcct ccctaactcc tgtgtatttt ccacagcctt tcccgggggg
     1261 gcggaaatcc gcactgcaga ggaggtaatt ccgatgtcct ccgaatatcc gtacaccctg
     1321 agggtccggt acggcgatac cgaccagatg ggagtcgcct attacgccaa ttatctctac
     1381 tggttcgaag tgggcaggac ggagttctgc cgcgcccacg gcaagtccta cgccgactgg
     1441 gaggaagagg gcattttcct tcccgtggtt gaatcccact gccgctacaa gcatccagcc
     1501 cggtacgagg acgagattgt cattttcacg aaaatcggtg aagtgaaggc atcgtccgtg
     1561 atttttgaat gccgcatcga acgggccgcc gacggcaagc ccctcgcctc gggatggacc
     1621 cgccatgcct tcgtggaccg gaatggaagg cttctgagag gagacaaccc cctgcgccga
     1681 tggatcgagg cgctggcggg atgacggagg tgtcgcgtac agtgagcacg aaagccatac
     1741 tgttgggaaa cgaggcaatc gccaggggaa ttgttgaagc ggggtgtctt gtggcgtccg
     1801 cctacccggg aacgccgtcg tccgaaatac tgcctgcggt ggcaaagttc gccgacgaac
     1861 tgggctcacc catggcggtc gaatggggcg tgaacgagaa ggtggccttc gaggtcgccg
     1921 ccgccgcctc ctttaccggc gcccgctcct gtgccgtcat gaagcaggtg gggctgaacg
     1981 ttgcggccga tcccttcatg agtgccgccc atttccagct caaggggggc atgctccttg
     2041 tggtcgcaga cgatccgggg ccccacagct cccagacgga acaggacagc cgctatttcg
     2101 ccatgttcgc caaggtgccc tgtttcgacc cctgttccgc cgccgaggcg aaggagatgg
     2161 tcttcgacgc cttcgacctg tcagagcggt tcggcatcat caccatgctc cgtcccagcg
     2221 tgagggtgga ccactgccgc caggacgttg aactcggaga gctccgccct ccgaaggggc
     2281 aggtgaagtt cgagaaggaa ccctcccggt gggtctgcct tcccgccagc gtgaaggtga
     2341 accatccccg gctgaacgcc aaggtggagg agatccgggc cgccttcgag acggagttcg
     2401 ggaagtacaa ctacgaagtt ccctcccgtg ggaagagcgt cctgggcatc gtggcctcgg
     2461 gagtgtgctt ctccgtggtc agcgacatcc tgaaggagtg gggccgggaa gacgtggctg
     2521 ttctcaagat cggcacgccc catcctctgc cggtgaagat ggtgaccgaa ttcatctccc
     2581 gccacccgaa ggtgctcttc ctcgaggaaa cctacccggt gatggaaatg cagcttcccg
     2641 acaggacggg aatcctcggc cgctggaacg gcgtcgtccc gggggccggg gaacttcttc
     2701 cggaggttat cgcgggcatt ctcgcgaaaa tcctcggcat ggaccagccc tccggggagg
     2761 gttcatccct tctcagggaa gctcttgaag atctgaagat cactccccgg aagcccatgc
     2821 tctgcccggg atgcccccac agggcgagct atttctccat cagg
//
LOCUS       DAOTHD010000179         2861 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_41424_length_2861_cov_3.270492, whole genome shotgun sequence.
ACCESSION   DAOTHD010000179 DAOTHD010000000
VERSION     DAOTHD010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41424_length_2861_cov_3.270492"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="P5219_04135"
     CDS             complement(<1..140)
                     /locus_tag="P5219_04135"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRX25976.1"
                     /translation="MKGLSEAFAGESMANRKYLAFAEQAEKEGYPATAKLFRTIAEAE
                     TL"
     gene            complement(234..1025)
                     /locus_tag="P5219_04140"
     CDS             complement(234..1025)
                     /locus_tag="P5219_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HRX25977.1"
                     /translation="MDILRRAASLITPEAWAELDEQAKKVLTANLSARKFVDVEGPKG
                     WSYSAHATGRLDVAQKQPKDGVQFGVNKVLPLVESRFTFDMDIWELDNISRGAKDPDL
                     SALEKAAKEIALFEEKAVYNGLPAAGIEGLSAAAGKRSIKIGGDDGQSLVNALSSAIF
                     NFSQDAVEGPYALVASPKVWKTIYTSVPGYPISKHVGNLVDKVILSAQEDSYLVSLRG
                     GDFELVLGQDLSLGFEERNGGKVRLFFAESFTFRVITPEAVIALK"
     gene            complement(1062..1409)
                     /locus_tag="P5219_04145"
     CDS             complement(1062..1409)
                     /locus_tag="P5219_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRX25978.1"
                     /translation="MQFTEPADILDQKTADEAKALKSLMEEVEAVNYYNQRVAATPNA
                     ELKELLAHNRDEEIEHAAMLIEWLRRNMEGWDKELKTYLFTSAPLLEVETAGEAGGGE
                     SGTPGGLGIGSLK"
     gene            1731..>2861
                     /locus_tag="P5219_04150"
     CDS             1731..>2861
                     /locus_tag="P5219_04150"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HRX25979.1"
                     /translation="MSLGRKMIILLAGFLAVAVTLTAGSYSLTARSVRARVEEAGNSL
                     VSETALSLDEYFSKLLSLTSSLSVSVSALSGNTVPPDCADLFGRYLDSVRSHGVQDVF
                     MGFENGDFSDATRWSPPEGYDPRIRSWYVEALKKEEAVITSPYRDLITGELIVSVTAP
                     VISPSGKLLGVFGLDVNLAHVQEKVISRRIFGEGIGFLVDRKGFFLASPFPEWDLSES
                     IAIPSTAVPSSLSTPAKALLLGEPGTAFITFRGESAMLFHSPAGDYFTFGVILPETVF
                     RRFVRDIAALHLFGGFVILLLAAVLLFPTVHGLRMSFASLSAVADGIAERLSGNRDIT
                     ETAFNVQVLGAEIGEAVEDSRVAEFKRFLRSLENALKIIGRQG"
BASE COUNT          542 a          815 c          783 g          721 t
ORIGIN      
        1 tggagggttt ccgcttcggc aatggtcctg aacagtttgg ctgtcgccgg gtagccttct
       61 ttctcagcct gctcggcaaa ggcgaggtac ttcctgttcg ccatggattc tccggcgaac
      121 gcttccgaga ggcctttcat cgtcttttcc atattctgct tcctccgtca atgtgaagtg
      181 ggttaaaaaa agcggtcccg ggggaccgct ttccggactg cggcatatgt atcctatttc
      241 agggcgataa ccgcctcagg ggtgatgacc ctgaacgtaa aggattcagc gaagaaaaga
      301 cggacttttc caccgtttct ttcttcgaag ccgagggaaa gatcctgtcc gaggacgagt
      361 tcaaaatcgc cgcctcgaag agatacaagg taggagtctt cctgggccga aagaatgacc
      421 ttgtccacaa gattcccaac gtgtttggaa atcgggtatc cgggaacact ggtgtaaatg
      481 gttttccata ctttcggcga agccacgagg gcataggggc cttcgacggc gtcctggctg
      541 aagttgaaaa ttgcggagga aagagcgttg acaagacttt gcccgtcgtc gcctccgatc
      601 ttgattgaac gctttcctgc ggctgcagaa agcccttcta tacccgcagc cggcaatccg
      661 ttgtacaccg ccttttcctc gaagagtgcg atttccttcg cagctttctc aagggcggaa
      721 aggtcaggat ccttcgcgcc gcggctgata ttgtcaagtt cccatatatc catatcgaag
      781 gtgaacctgc tttccacgag aggaagcacc ttgttgacgc cgaactggac gccgtccttg
      841 ggctgcttct gggccacgtc cagcctgccg gttgcatggg cgctgtagga ccatcccttc
      901 gggccttcca catccacgaa ctttcgtgcg gaaagatttg cggtgaggac tttttttgcc
      961 tgttcgtcaa gctccgccca tgcctcgggg gttatgagag aggctgctct tctcagaatg
     1021 tccatagtat tgacctcctt tgtactcggg tatctcaggt actacttcag gctgccgatg
     1081 cccaggccgc ccggtgtgcc gctctctccg cctccggcct cgccggcggt ttcgacttcc
     1141 aggagggggg ctgaagtgaa gaggtacgtt ttgagttcct tgtcccaccc ctccatgttc
     1201 cggcgaagcc actcgatgag catggcggcg tgttcgatct cttcatccct gttgtgggcc
     1261 aggagctcct tcagttcagc gtttggagtg gcggccactc tctggttgta gtagttgacg
     1321 gcctccactt cttccatgag gctcttgagg gccttcgcct cgtctgctgt cttctggtcg
     1381 agaatatccg caggttccgt gaactgcatg gtatctctcc tttcttgtct ttcaccgggg
     1441 ggtgcttcaa tccgccctca ccggcatgat tttccatatt ataccctgag cccgggtaaa
     1501 tggagctctt tgaaatgcga aagattgtta ctttgtaatt gttttaaatg gcatgacgac
     1561 agtatagcaa aaaaaaaggc cccgtcaata ttagtcaggt gaaaaaggaa gcttttcctg
     1621 tgttagaatt ccataacatg tgcgcttttt tgccttcctg cagcggaatg aagacccgtt
     1681 tccccggcat ttccgccgga ctgccggggg gcacactgag aggtgaccca atgtccctgg
     1741 gcagaaagat gataattctc cttgccggtt ttcttgccgt cgctgttacg ctcactgcgg
     1801 gaagctactc cctgactgcc cggagcgtgc gggcaagagt ggaggaagcc ggaaacagcc
     1861 tggtttcaga gactgccctt tccctcgacg agtatttttc caagctcctt tccctgacct
     1921 cgtccctttc ggtttccgtg agcgcccttt ccgggaacac tgttccgccc gactgcgcag
     1981 acctgttcgg ccggtatctt gactctgtcc gttctcatgg ggtgcaggat gtgttcatgg
     2041 gcttcgaaaa cggtgatttt tccgatgcca cccgctggag tccgccggag gggtatgacc
     2101 cccggatccg gtcatggtat gtagaagccc tgaaaaaaga agaagcggtc atcacctccc
     2161 cctaccgcga cctgatcacc ggagagctca tcgtgagcgt caccgcgccg gtgatttctc
     2221 cctcgggaaa gctcctggga gtcttcggcc tggacgtgaa tctcgcccat gtccaggaaa
     2281 aagtgatctc ccgcaggatc ttcggcgagg ggatagggtt cctcgtggac agaaagggtt
     2341 tttttctcgc gtcccccttt ccggaatggg atctctcgga gagcatcgcc attccttcga
     2401 cagcggttcc ctcctccctc tcgacgcccg caaaggcact gctcctcgga gaacccggaa
     2461 ccgccttcat cactttcaga ggcgaaagcg ccatgctttt ccactcccct gctggggact
     2521 attttacctt cggggtgatc ctccccgaga cggtattccg ccgtttcgtc cgggacatcg
     2581 ccgccctcca tctcttcgga ggattcgtca ttctccttct cgcggcagtg cttctgttcc
     2641 cgaccgtgca tgggctcagg atgtcctttg cctccctttc cgcagtcgcg gacggaattg
     2701 cggagcgtct ttccggaaac agggacataa cggagacggc cttcaacgtc caggtcctgg
     2761 gagcggaaat cggcgaggcc gtggaggatt caagagtggc ggaattcaag cgctttctca
     2821 gaagccttga aaatgccctg aagatcatcg ggaggcaggg a
//
LOCUS       DAOTHD010000180         2847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_41667_length_2847_cov_3.210602, whole genome shotgun sequence.
ACCESSION   DAOTHD010000180 DAOTHD010000000
VERSION     DAOTHD010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41667_length_2847_cov_3.210602"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..880
                     /locus_tag="P5219_04155"
     CDS             23..880
                     /locus_tag="P5219_04155"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRX25980.1"
                     /translation="MIRSLPSSRRYRWSFNLFIGAYFVLLFAPLVVTMILAFNDSMYP
                     SLPWEGFTLDWFFGSGPEKFGIFNDQANLRSLAFSFRVALTVACLSTILGTCAAFLFE
                     QENFRFKGALYFLMIAPLVIPGVILGISILMFANTVGIFIEDLTGIYVKSLVPGFWLV
                     VLGQSSFISTIVALVVSARLKKFDRSLEEAAYNLGANRAEVLRYVTLPYLRPSILGGA
                     AVAFLMSFENFNTTIFTVGSQATLPINMYMQVRDGSTPVINAISFLLITGTSIFAVAN
                     LYLGKKKED"
     gene            complement(908..1624)
                     /locus_tag="P5219_04160"
     CDS             complement(908..1624)
                     /locus_tag="P5219_04160"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HRX25981.1"
                     /translation="MLVLGHRGASGYAPENTIAAFGLGISQGCHGFEFDVQLSRDGEP
                     VVHHDWSVERTTGGTGDIRDLTFREIRSLDAGKWFSPEFRGQKIPALAEVLELIPEKM
                     LLNVELKSRASDGPGLEEKTISVLQKHGRLENTILSSFNHLSLERARDIAPGIRLGML
                     YEALLLRPFDYVEKLDLGLYSIHPLHDYVSPALVLETHRRGLKMACWTVNTRERTAEL
                     REYGVDIVITNYPDRCLELE"
     gene            1759..2511
                     /locus_tag="P5219_04165"
     CDS             1759..2511
                     /locus_tag="P5219_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRX25982.1"
                     /translation="MEHSLFLWTGIFLVGIIAGFFNTIGGGGSLLVLPFLSFLGMDVA
                     VANGTNRLAIVLQSIAGAEAYRKKGVLCFRTALPLAGITTFGSVFGTFLAITIDRQYL
                     NLSVAAFISLMAILLVFKPEMWEKQHETRLSKAGIFLVFFAIGMYGGFIQAGVGFFLT
                     WGLALAVGVDLVGGNAIKMVIVGLYNVISLAIFYRNGMVDIPVGLIMAGGSMVGAVIG
                     ARFAVAKGNTWIRWILAAVVIVSAVKMVFDAL"
BASE COUNT          526 a          794 c          831 g          696 t
ORIGIN      
        1 aaaacctggg ggaggtggtc aaatgatccg gtctcttccg tcgtcccgca ggtacaggtg
       61 gagcttcaac ctgttcatcg gggcgtattt cgttcttttg ttcgcccccc tggtggtgac
      121 catgatcctc gccttcaacg actccatgta cccatccctt ccatgggagg ggttcactct
      181 cgactggttt ttcggcagcg gcccagagaa gttcggcata ttcaacgacc aggcgaacct
      241 gcggagcctg gccttttcgt tccgggtcgc cctgacggtt gcgtgtctct ccaccatcct
      301 cggcacgtgc gcagcctttc tcttcgagca ggagaatttc cggttcaagg gggcgctgta
      361 tttcctcatg atcgccccgc tggtcattcc gggcgtcatc ctcggcattt ccatcctcat
      421 gttcgccaac accgtgggaa tttttatcga agacctcacg ggaatctacg tgaaatccct
      481 cgtaccgggc ttctggctgg tggtcctggg gcagtcatcc ttcatctcga ccatcgtggc
      541 ccttgtggtc tcggcgcggc tgaagaagtt cgaccgctcc ctggaggagg cggcctacaa
      601 cctgggggcg aacagggcgg aagtgctccg gtacgtcacg ctgccgtacc tccgtccgtc
      661 tatattgggg ggagcggccg tggcgtttct catgtccttc gagaacttca acacaaccat
      721 tttcaccgtc ggatcccagg cgaccctgcc catcaacatg tacatgcagg tccgcgacgg
      781 ttccacgccg gtgatcaacg ccatttcatt tctactcata acaggtacgt ccatttttgc
      841 cgtggccaac ctgtaccttg gaaagaaaaa agaggactag ctctcccttc cggcctctcc
      901 ggagcggtta ttcaagttcc aggcacctgt cagggtagtt ggtgatgacg atgtccaccc
      961 cgtattcccg aagctccgcg gtgcgctccc ttgtattcac cgtccagcac gccattttca
     1021 gcccgcggcg gtgggtctca aggacaaggg caggggagac gtagtcgtgc agggggtgga
     1081 tgctgtagag gcccaggtcc agcttttcca cgtagtcaaa cggcctgagg agcagcgcct
     1141 cgtagagcat gccgagacgg atgccggggg cgatgtccct tgcccgttcc agggagagat
     1201 ggttgaagga agagagtatg gtgttttcga gccttccgtg tttctgcaga acagagatgg
     1261 tcttttcctc gagccctggc ccgtccgatg cccggctttt cagctccacg ttcaggagca
     1321 ttttttcggg aatcagttcc agcacttcgg caagggcggg gattttctgg ccccggaatt
     1381 cgggggagaa ccatttcccc gcatcaaggc tccggatttc gcggaatgtc aggtcgcgga
     1441 tgtccccggt gccgcctgtg gtccgctcca cggaccagtc gtgatggacg actggttctc
     1501 cgtcccggct cagctggacg tcaaactcga aaccgtggca cccctggctg atgcccagcc
     1561 cgaaagccgc aatcgtgttt tccggggcgt accccgaagc tcccctgtgt cccagaacaa
     1621 gcatttatct gtcacttctt tcacgtggat gtggctgcat cttttatgta tcataagtcg
     1681 ctgaaagaaa aaagaggaaa cgagagtccc ggggaagttt accccgggaa aaattatact
     1741 tgaggacgtg atcgggacgt ggaacattct ctctttttgt ggaccggaat ctttctcgtg
     1801 gggatcatcg cgggattttt caacaccata ggcggaggag ggtctttgct ggttctccct
     1861 tttctctctt tcctggggat ggacgtagcg gtcgcgaacg gcacgaaccg gctcgccatc
     1921 gtgctgcaga gtatcgcggg agctgaggcg taccggaaaa agggcgtgct gtgcttccgg
     1981 acagctcttc ccctggccgg aatcacgacc ttcggttcgg tcttcgggac ctttctggcc
     2041 ataaccatcg acaggcagta cctcaatctt tccgtggccg ccttcatctc cctcatggca
     2101 attctcctgg tgttcaagcc tgagatgtgg gaaaagcagc atgagacccg cctgtcgaag
     2161 gccggaattt ttcttgtttt cttcgccatc ggcatgtacg gaggcttcat ccaggccggg
     2221 gtaggcttct ttctcacctg ggggcttgcc cttgccgtcg gggtggatct ggtcggggga
     2281 aatgccataa agatggtgat cgtgggccta tacaatgtaa tcagccttgc cattttttac
     2341 aggaacggca tggtggatat tccggtaggc cttattatgg ccggaggaag catggtcggc
     2401 gcggtcatcg gggccaggtt cgctgtggcg aaagggaaca cgtggatccg gtggatcctg
     2461 gccgccgtgg tcattgtcag tgccgtgaag atggtcttcg acgccctgta gcggacccta
     2521 tcgccggagc tgcatgtctc ccggcttctt tgaccggagg caaatgccct tctgccgctc
     2581 ctgcgggaag aatgccgttc cggagcggga aggaacagaa taacacggga ttgttgtggg
     2641 gatgaatgag tgagataatc gggcaggata ggcggataga agaaaaagga gggattctcc
     2701 gtgaaaagaa gcctgtttcc tctcgccctg ctggtttttt tctctctttt ccccatcgct
     2761 gcgtcagctc agtcgcctct cgttggtcat tattttattc cggagtatta ttccaccgtg
     2821 gaggaagctg tgggacggct caagaac
//
LOCUS       DAOTHD010000181         2847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_41668_length_2847_cov_3.113181, whole genome shotgun sequence.
ACCESSION   DAOTHD010000181 DAOTHD010000000
VERSION     DAOTHD010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41668_length_2847_cov_3.113181"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="P5219_04170"
     CDS             complement(<1..204)
                     /locus_tag="P5219_04170"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048552.1"
                     /note="Catalyzes first step of the de novo purine
                     nucleotide biosynthetic pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRX25983.1"
                     /translation="MCGIFGAYSPMGKKVLEDVYLGLYALQHRGQEAAGVAWVNSQGR
                     ISSIKGAGLVHLALNQAELAAVET"
     gene            complement(205..2358)
                     /gene="purL"
                     /locus_tag="P5219_04175"
     CDS             complement(205..2358)
                     /gene="purL"
                     /locus_tag="P5219_04175"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HRX25984.1"
                     /translation="MDFSKYGIRKEEFAAAEAALGREPNECELRILGVMWSEHCSYKS
                     TKHLLKHFPTRGPKVVLGPGENAGIVDLGDGLGAAFKAESHNHPSAVAPYQGAATGVG
                     GIIRDILALGARPVASMDGLFFGDPEHPRTGHLSAGIVKGVGDYGNAVGVPTVGGKTV
                     YDPCYNENPLLNAFCIGLVELDKIVSSQTARPGQLVVLLGSKTGRDGIAGAAFASVEL
                     SEDAKESRPSIQIGDPFAEKMLIEACLELKEKNLIVSMQDMGAAGITSSSSEVAAKSG
                     VGMKLHFDRVPLRAADMEPWEIALSESQERMLLIVAPEHMEEVSSVARKWELDCAVIG
                     ETEEGDDYSIFFHGEKVASLPATLIGDGCPPIHWPSEKPADFETRWDFDLDGLPAPGD
                     WNAAMLSLLGNPSMTPKRWIFEQYDSMVQLNTVSGPGHPVSVLRIKGRESLIGLVFDA
                     DPWKCCLDPFRGGAETVARTVRALSVAGAEPLGLTDCLNFPSPEVPGQYWALEECIEG
                     MAAACGALGCPVVSGNVSLYNETKSGAILPTPVVGTVGLIPSPEDFLPCGAWEEGDLL
                     FLVGPCNPSLAGSLYIRSLGRPEAGRPLEFSGEAEKDFSERALRTARKKAAHSGRALA
                     GGGLAAALAKDSAESGCGARIALGIPTRKDVVLFGEGGARALYSVPMSRVPLFKAIWS
                     GYPCLELGRAGGDVLSVGGAFSLAVSRIQEIWRNN"
     gene            complement(2345..>2847)
                     /gene="purQ"
                     /locus_tag="P5219_04180"
     CDS             complement(2345..>2847)
                     /gene="purQ"
                     /locus_tag="P5219_04180"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HRX25985.1"
                     /translation="KEHAGQGGLVLGICNGFQILTESSLLPGVLLPNKTLSFICRPCF
                     LSVENTDTPFTGLFEKGQVVQFPIAHGDGLFFLPADELNDLENRGGAVFRYVSPGGVG
                     GDEWNPNGSVNSIAGIVNPQGNILGLMPHPERASEAILGGEDGRLFWRSVASFLEKGG
                     RRHGLL"
BASE COUNT          561 a          880 c          832 g          574 t
ORIGIN      
        1 tgtctccacg gctgcaagtt ccgcctggtt cagggccagg tggaccagac cggctccctt
       61 gatggaggaa atccggccct gggaattgac ccatgccact cccgcagcct cctgccctct
      121 atgctggaga gcgtagaggc caaggtaaac atcctccagg acctttttgc ccatggggga
      181 gtaggcgccg aaaataccgc acattcagtt attcctccat atttcctgta ttcgggaaac
      241 cgccaggctg aaagcacccc cgacggaaag gacgtcaccg ccggcacggc ccagttcaag
      301 gcagggatat ccgctccaga tcgctttgaa gagaggcacc ctggacatgg gaacggaata
      361 cagcgcccgg gctcctccct cgccgaagag aaccacgtct tttcttgtgg ggatccccag
      421 ggcgatccgg gccccgcagc cgctttccgc cgagtccttc gccagggcgg cggcaagtcc
      481 tcctccggca agagcccgtc cgctgtgtgc cgcttttttc cgggccgttc tgagggccct
      541 ctcagaaaag tccttctcgg cctcaccgga gaattcaaga ggccgccctg cctccggcct
      601 cccgagagac cggatgtaca ggctgcccgc gagggatggg ttgcaggggc ccacgagaaa
      661 gaggaggtcc ccttcttccc aggcgccgca gggaaggaaa tcttcggggg aaggaatgag
      721 tcccaccgtt cccaccacgg gagtgggcag aatggctccc gatttcgttt cgttgtagag
      781 gctgacgttc cctgagacca cggggcagcc gagggctccg cacgctgccg ccattccctc
      841 gatgcattct tccagggccc agtattgtcc gggaacctcg ggggacggga aattgaggca
      901 atccgtcagc ccgaggggct cggcgccggc aaccgaaaga gcccgtacgg tccgcgcgac
      961 cgtttccgct cctcccctga acgggtcgag acagcatttc cagggatccg catcgaaaac
     1021 caggccaatg agagattctc ttcccttgat tctgaggacg ctcaccggat gtcccggccc
     1081 gctgaccgta ttgagctgca ccatggagtc gtactgttcg aaaatccagc gtttgggggt
     1141 catggacggg tttccgagaa gggaaagcat cgcggcattc cagtctcccg gagcgggaag
     1201 tccgtcaagg tcgaaatccc atcttgtttc aaaatcggcg ggcttttccg acggccagtg
     1261 aatgggggga cacccgtccc ctatgagcgt ggcgggaagg gaggcgactt tttctccatg
     1321 gaagaaaatg gagtagtcat ccccctcctc tgtttcaccg atgaccgcgc agtcaagttc
     1381 ccatttccgc gcgaccgagg agacttcttc catgtgctcc ggggcgacga tgagcagcat
     1441 tctttcctgg gattcggaga gggcgatctc ccatggctcc atgtccgcag cccgaagagg
     1501 caccctgtcg aagtggagtt tcatgccgac accgctcttc gccgccacct cgctcgagga
     1561 tgatgtgatt ccggcggcac ccatatcctg catgctgaca atgaggtttt tctccttcag
     1621 ttcaagacat gcctcgataa gcattttttc agcgaagggg tcgccgatct gtatggaagg
     1681 cctgctttcc ttcgcgtcct ccgaaagttc caccgatgcg aaagcggcgc cggcgatgcc
     1741 gtcacggccc gttttggagc cgagaagaac cacgagctgc cccggtcggg cagtctggga
     1801 actgacgatc ttgtccagct ccacgaggcc gatgcagaag gcgttgagaa ggggattttc
     1861 gttgtagcat gggtcgtaaa ctgtttttcc tccgacggtg gggacaccca cggcattccc
     1921 gtagtctccc acccccttca cgattcccgc agacagatga ccggttctgg gatgttcagg
     1981 gtctccgaag aagagtccgt ccatggaggc cacaggcctg gccccaaggg cgagaatatc
     2041 ccgtatgatg cctccgaccc cggtggccgc tccctggtag ggcgccaccg ctgacgggtg
     2101 gttgtggctt tccgccttga acgccgctcc cagaccgtcc ccgaggtcga cgattccggc
     2161 gttttcaccg ggtccgagga cgaccttcgg cccccttgtg ggaaaatgct tcaaaaggtg
     2221 tttcgtggac ttgtagctgc agtgctcaga ccacattaca ccgaggatcc tgagttcgca
     2281 ttcattgggc tcccttccga gggcggcttc ggcggcggcg aattcttctt tcctgatgcc
     2341 gtatttagag aagtccatgg cgcctgcctc ctttctcaag gaaagaagcg acggaccgcc
     2401 agaaaagccg gccgtcctct cctccgagaa tggcctcgct cgcccgttcc gggtggggca
     2461 taaggccgag aatattaccc tggggattga caatgcccgc gatgctgttc acggatccgt
     2521 tgggattcca ctcatccccc ccgacaccgc cgggggaaac atagcggaac acggctccgc
     2581 ccctgttttc aaggtcgttg agctcatccg ccggcaagaa gaaaagcccg tccccgtgag
     2641 cgatgggaaa ctgaaccacc tgcccttttt cgaaaaggcc cgtaaaggga gtatccgtgt
     2701 tttccacaga caaaaagcag ggcctgcaga taaatgaaag ggtcttgttg ggcagcagga
     2761 ctccaggaag aaggctggat tcggtcagta tctgaaaccc gttgcagatg cccagaacaa
     2821 gccccccctg cccggcatgc tccttta
//
LOCUS       DAOTHD010000182         2842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_41761_length_2842_cov_2.997488, whole genome shotgun sequence.
ACCESSION   DAOTHD010000182 DAOTHD010000000
VERSION     DAOTHD010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41761_length_2842_cov_2.997488"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="P5219_04185"
     CDS             complement(<1..964)
                     /locus_tag="P5219_04185"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRX25986.1"
                     /translation="MSIYLLVFFIFFLVIGVPIAVALGLTCIFSVLVTGAFGVEVLIQ
                     KTFNAGNSFALMAIPFFILAGNIMAAGGVSRRLVNLAGALFGRMSGGLALVATLASTF
                     FGAVSGSAPATTAAIGGVMLGPMGEKGYDKNFAGAVVAASGTIGLIIPPSLTMVLYGV
                     TTGVSIGEMFIAGVIPGIIICIVLMVVEYFISLKRGYKGDEAASFKTVSKYFRESVFA
                     LFMPVIILGGIYAGIFTPTESAAVAVVYGLIVGIFIHKELSWKDVSKLILKSAKSTAL
                     VMYLMVTAEILSYVLVSEQIPQTIAASILNISTNAIVVQLIMVFI"
     gene            complement(961..1464)
                     /locus_tag="P5219_04190"
     CDS             complement(961..1464)
                     /locus_tag="P5219_04190"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRX25987.1"
                     /translation="MLKLLKYVNDGITRVMNWLMVTLMSLMVAFIFAQVIFRYVLEKP
                     LSWSEELSGYLFSGVFFFGSVLLYRESRHINMSIVVDSMKNEFARKLLVVAAHLFSFF
                     FLAVMVWYSYPMAMQILEFEVVSPSMEWLKIGYVFLIVPAASFLSLAVLFEVILKSLA
                     DLKEKQS"
     gene            complement(1582..2562)
                     /locus_tag="P5219_04195"
     CDS             complement(1582..2562)
                     /locus_tag="P5219_04195"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRX25988.1"
                     /translation="MRRRLLLGALVVLFAFMAVSAAFAAPKVIKIGHTGTKDHHYQMY
                     LETWAEKVSAATDNRYTFEIYPSDLYGKPNQLIEGAQLGTTDMVLATGSLLTSYNPKI
                     GVLSLPFIFKDSQEAHDILNGPVGKEFEEGLAKRNLIILGWWENGMRHLFPPKPVNRI
                     EDLKGLKLRVINSAEMIDTINAFGASAVPMAFNDVYSAWQLGTIDGCEGTTTHMLTQK
                     YFELTKSSALLWYMHVSNPLVASKRLWDSIPDADKAVFLDEAKKMSAFSFDFQRQMDE
                     KEIKQCEELGVVFTRPDREPFVKAVAPVYEKFRPKYGDMIDKIQAITQKK"
BASE COUNT          702 a          791 c          748 g          601 t
ORIGIN      
        1 ggatgaacac catgatgagc tgcaccacga tggcattggt ggagatgttg aggatggagg
       61 ccgctatcgt ctggggaatc tgctcgctca ccaggacata gcttaaaatc tccgccgtga
      121 ccatgagata catcacgaga gcggtggact tggccgattt cagtatgagc ttcgatacat
      181 ccttccacga gagttcctta tggatgaaaa tgccgacgat cagtccatag acgacggcaa
      241 ctgccgccga ttcagtgggc gtgaagattc cggcgtaaat tccccccagg atgatgaccg
      301 gcatgaagag ggcaaaaacc gactcccgga aatactttga aaccgtcttg aaggaagccg
      361 cttcatcccc cttgtatcct ctcttcagcg agatgaagta ttccaccacc atgagcacga
      421 tgcagatgat gattccggga ataactccgg cgataaacat ttcgccgatg gatactcctg
      481 tcgtcacccc gtacaggacc atggtgaggc tgggcggaat gatgagccct atggttcccg
      541 aagcggcgac gacggctccc gcgaagtttt tgtcataccc cttctccccc atgggaccga
      601 gcatgacacc gcctatggcg gccgtcgttg cgggcgcgga gcctgatacc gcgccgaaaa
      661 atgttgaggc cagcgtcgcc acgagggcaa gcccgccgct catccttccg aaaagggccc
      721 cggcaaggtt caccagccgc ctggagacgc cccccgccgc cataatattt cccgccagga
      781 tgaagaaggg aatggccatg agggcgaagg aattcccggc attgaaggtc ttctggatga
      841 gaacctccac gccgaaagca ccggtgacca gcactgagaa gatgcatgtc agaccgaggg
      901 cgacggcgat gggaacgcct atgacgagaa aaaagataaa aaagaccagc aggtagatac
      961 tcatgactgt ttctccttca ggtcagccag ggatttaagg atcacttcga acagcacggc
     1021 cagggacaaa aagctcgctg ccggcactat caggaacacg tagccgatct tcagccattc
     1081 catcgaagga gacacgactt cgaattcgag aatctgcatg gccatgggat aggagtacca
     1141 gaccatcacc gcaaggaaga aaaatgaaaa gagatgggca gccaccacca gaagtttcct
     1201 ggcgaattcg ttcttcattg agtccaccac gattgacata ttgatatggc gggactccct
     1261 gtaaagaaga acggaaccga agaaaaacac tccggagaag aggtagccgg aaagttcctc
     1321 agaccaggaa agcggttttt ccaggacata ccggaagatg acctgggcga agataaacgc
     1381 caccatgagc gacataaggg tgaccatcag ccagttcatc accctggtga taccatcgtt
     1441 gacatatttc agcagtttca gcatacaacg acctcccgat cccggaacat agaataatca
     1501 ctcgtccttt caggaaaaga gcggcggtca gggaaccttt tccgggctcc cggaccgccg
     1561 atcctccctg tttctcccag gctatttctt ctgtgtgatg gcctggattt tgtcgatcat
     1621 atcgccgtat ttcgggcgga atttttcata cacaggagcg actgccttta caaacggctc
     1681 cctgtcggga cgggtaaaca cgacgccgag ctcttcgcac tgcttgatct ccttttcatc
     1741 catctgcctc tggaaatcga aggaaaaagc gctcatcttc ttcgcctcgt cgaggaagac
     1801 cgccttgtct gcatcgggaa tggaatccca cagtctcttc gaggccacga gaggattgga
     1861 gacgtgcatg taccacagga gcgccgaact cttcgtcagc tcgaagtatt tctgcgtgag
     1921 catatgggtc gtcgttccct cgcagccgtc gatggttcca agctgccatg cggagtagac
     1981 atcgttgaac gccatgggca ccgcgctggc accgaaggcg ttgatggtat cgatcatctc
     2041 ggcggaattg atgaccctca gcttcagccc cttgaggtcc tcgatacggt tcaccggttt
     2101 cggggggaag agatggcgca tgccgttctc ccaccagccg aggatgatga ggtttctctt
     2161 cgccaggcct tcctcgaact ccttgcccac gggcccgttc agaatgtcgt gggcctcctg
     2221 ggaatccttg aagataaagg gaagactgag aacccctatt ttggggttat aggacgtgag
     2281 aagcgacccc gtggccagga ccatgtccgt cgtcccgagc tgggcgcctt ctatgagctg
     2341 gttcggcttc ccgtagagat ccgaaggata aatctcgaat gtatagcggt tgtccgtcgc
     2401 tgccgaaact ttttccgccc atgtctccag gtacatctgg taatgatggt ccttggtccc
     2461 cgtgtggccg atcttgatca ccttcggcgc ggcaaaggcc gccgaaacgg ccatgaacgc
     2521 aaacaacacg acgagcgctc ccagcaacag tcttcttctc acagataacg cctccctctc
     2581 ttgcagcagg aatgtgggaa ataggacctc ccttgtatga gtcctcagtc tacatgcacc
     2641 atcgatgtct gtcaaatgca aactaaatta ctattcaaat ttgcgaatat taattccttc
     2701 gagctatttt gcatcgcaaa aaataacaaa gccaatctcg gaatacgggc tgccggacac
     2761 cagtcctaga agaaaaaggt tccccggagg acgacacgtg ccttgcctcg gatttccacg
     2821 gcgtcgtctc cgacttccag aa
//
LOCUS       DAOTHD010000183         2841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_41786_length_2841_cov_2.400574, whole genome shotgun sequence.
ACCESSION   DAOTHD010000183 DAOTHD010000000
VERSION     DAOTHD010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41786_length_2841_cov_2.400574"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="P5219_04200"
     CDS             <1..189
                     /locus_tag="P5219_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25989.1"
                     /translation="DVYDALRSPRVYKDSLSHEDAVRIILSGDGRTEPSHFSPEILFF
                     FRNSHREMDRIFKSRRTR"
     regulatory      226..345
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            438..1262
                     /locus_tag="P5219_04205"
     CDS             438..1262
                     /locus_tag="P5219_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRX25990.1"
                     /translation="MRKMRSLLFVCLFVFALAGAASGAEVLRMATTTSTDNTGLLDYL
                     APLLLQEKNIELQWVSVGTGKALEYGRNGDVDVLLVHAPEVEKKFVAEGCGVNRRQVM
                     FNDFIVVGPASDPAGIKGMAAAPALKKIAATAAPFASRADKSGTHVAELTLWKNAGIP
                     EPDKEKWYIQTGQGMLETLMIADERKGYCLTDRGTFIKYEDSRKEQGGLVIIVEGDDS
                     LKNQYSVIAVNPAKWENIRYDLAVALIDWMVSPSTQKAIGDFQLLGKQLFFPNAER"
     gene            complement(1314..1763)
                     /locus_tag="P5219_04210"
     CDS             complement(1314..1763)
                     /locus_tag="P5219_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRX25991.1"
                     /translation="MTQPLAPCASVDRMMRDMRRSFGEFSSDMGYSQETERVPAIPRG
                     DFYRKDGKVCAEFELPGIDPSKVELNVFEDRLTLQAEKSDEKTVDENDILRSERYYGK
                     VSRSIQFPMEVDPDTAKAAFKNGVLTVEVSEKVQAEKMKKVAIEEKA"
     gene            2010..2591
                     /locus_tag="P5219_04215"
     CDS             2010..2591
                     /locus_tag="P5219_04215"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRX25992.1"
                     /translation="MKKDIGISTPLYPAPALVVAAYGTDGRPGGLMVAWGGVCNSEPP
                     CIAVAVAKSRHTLEGIRSRKAFTVNIPSEDQAAEADFFGLASGRDTDKFSAAGLTPVQ
                     GEIADAPMIGEFPVSMECEVVQSLDLGSHFLFVGKVVKTWAREECLDEKGYPDPEKVR
                     PLVFAPRTGKYFSLGGMVGKAYGEGKKYLKGND"
BASE COUNT          632 a          803 c          802 g          604 t
ORIGIN      
        1 gacgtgtacg acgccctccg gtccccaagg gtctacaagg attcgctgtc ccatgaggat
       61 gcggtccgca tcatcctgtc cggcgacggc cggacggaac ccagccattt cagccctgag
      121 atcctctttt ttttcaggaa cagccacagg gaaatggaca ggatattcaa aagccgccgg
      181 acgaggtaac aaaacaggtc cttccgtggt atcatggcaa caaaagaata gatgtatctc
      241 cgagcccgtc cgcctacagg ctattcccac ggcggcgggc cgtcagggtc cgcccggaaa
      301 caggcgggcc tcccgtgttc ggcaaggaga taacggactc tccggagccg ttccctgccg
      361 tccatggcag tggaacggct tttttacgtt gcttcgtgtt cagtcacacc acataaaagg
      421 aggaaataac agtgaaaatg agaaaaatgc ggtcgcttct gtttgtctgc ctgttcgttt
      481 ttgcccttgc cggggccgct tccggagccg aggttctccg gatggccacc accaccagca
      541 cggacaacac tggtcttctt gattacctgg cccccctgct gctgcaggaa aagaacatcg
      601 agctccagtg ggtttccgtc ggtacgggca aggctctcga gtacggccgg aacggcgacg
      661 tggacgtcct tctggtccat gcccccgagg tggagaagaa attcgtcgcc gaaggatgtg
      721 gggttaaccg gcgccaggtg atgttcaacg acttcatcgt ggtgggaccc gcttccgatc
      781 ctgcgggaat caaggggatg gccgccgccc ccgccctgaa gaaaatcgcc gcaacagcgg
      841 ctcccttcgc cagccgggcc gacaagtcag gaacccacgt ggcggagctt accctctgga
      901 aaaacgccgg gatccccgag cccgacaagg aaaagtggta catccagacc ggccagggaa
      961 tgcttgaaac cctcatgatc gccgatgagc gcaaaggcta ctgcctcacc gacaggggga
     1021 ccttcatcaa gtacgaggac tcccggaagg aacagggcgg actggtgatc atcgtcgagg
     1081 gcgacgactc cctgaaaaac cagtacagcg tcatcgccgt caatccggcc aagtgggaga
     1141 acatcaggta cgaccttgcc gtggccctca tcgactggat ggtctctccg tcaacccaga
     1201 aagccatcgg agacttccag cttctcggaa agcagctctt tttcccgaac gccgaaagat
     1261 aaaaccgaag gagcgccggc ggccccggcg ctcctatttt ccaactccgc ttcctatgct
     1321 ttttcctcta tggccacttt cttcatcttt tccgcctgga ctttttccga aacctccacg
     1381 gtgagcaccc cgtttttgaa cgccgctttc gctgtgtcgg gatcaacttc catggggaac
     1441 tggatggacc gggaaacttt tccgtaatat cgctccgacc ggagaatgtc gttctcgtcc
     1501 accgttttct cgtcggattt ttctgcctgg agagtcagtc ggtcctcgaa aacattcagc
     1561 tccacttttg aagggtcaat gccgggaagc tcaaattccg cgcagacttt tccgtctttc
     1621 cggtaaaaat cccccctcgg tattgcggga accctttcgg tctcctggga atatcccatg
     1681 tcggaggaaa attcaccgaa tgaacggcgc atgtccctca tcatcctgtc cactgaagcg
     1741 caaggagcca agggttgggt catgtcattg ggcccacctc caaaaatctg atgaatataa
     1801 taatgaccat aaggtcaata ttatattcat cagtattggt tagacaaggt gggcaggaag
     1861 aaaataggga gaaataacgg aaattagcgc cgtttttttg ctttttcctt tggatatctc
     1921 tatttttctt gtattttctc cgaccgggcg gggaagataa ccctgcaggt gtacaatgag
     1981 cccgagaata ccggcaagga gtgtgtgcta tgaaaaagga cataggaatt tccacccctc
     2041 tttatcctgc ccctgcactt gtagtggcgg cctacggaac cgacggacgc cccggcggtc
     2101 tcatggtggc ctggggaggc gtctgcaatt ccgaaccgcc ctgcattgcg gttgccgtgg
     2161 cgaaatccag gcataccctt gaggggatac ggtccaggaa ggcttttacg gtaaacatcc
     2221 cgtccgaaga ccaggctgcg gaggccgatt tcttcggcct cgcctcgggg agggatacgg
     2281 acaagttttc cgcggcgggg ctcaccccgg tgcagggaga gatcgccgat gcgcccatga
     2341 tcggggaatt tcccgtctcc atggagtgtg aagtggttca gtcccttgac ctcgggtccc
     2401 atttcctctt cgtgggaaag gtggtgaaga cctgggcccg ggaagaatgc ctcgacgaaa
     2461 aaggataccc ggatcctgaa aaagtgcgcc ccctcgtctt cgctccccgc acggggaagt
     2521 atttctccct cggcggcatg gtaggcaagg cgtacggtga aggaaagaaa tacctgaagg
     2581 gaaacgactg aggatcccct gccctttttc ctgaaaatga aagaggagcc ccggaaataa
     2641 aagccgggac tcctcttttt tgacggaagg acgctgctag atcgtgccgg ccccttcagg
     2701 cttcggacgg aggaggagga acaggccgat gaccgccgcc gtgctgccaa gggaaatggt
     2761 caggttgccg tggagggcca gaagtccgaa ggctgcgaaa gccagtcttt cgggcagtga
     2821 gagtttgcgc tttgtgaagc a
//
LOCUS       DAOTHD010000184         2837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_41854_length_2837_cov_2.988138, whole genome shotgun sequence.
ACCESSION   DAOTHD010000184 DAOTHD010000000
VERSION     DAOTHD010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41854_length_2837_cov_2.988138"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="P5219_04220"
     CDS             complement(<1..431)
                     /locus_tag="P5219_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HRX25993.1"
                     /translation="MTMQKLDFLLEESRKGRPMTLALACPYGDDALAAVTRAGREGVV
                     KAVLVGDAARIRTLAGQDGCDLSGVTIVDEKDDEKAVERSVRMVSSGEADLLMKGLVK
                     TSTLLKAVLDKEWGLRTGSLLSHLVLFEIPAMNRRVIGISDG"
     gene            complement(428..1522)
                     /gene="buk"
                     /locus_tag="P5219_04225"
     CDS             complement(428..1522)
                     /gene="buk"
                     /locus_tag="P5219_04225"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HRX25994.1"
                     /translation="MAFQILAINPGSTSTKIAWYSDGTETWRETVRHDPDALAAFPSV
                     ADQFSFRLETIAEALASHGASFDELSAVAGRGGLVEPIPGGTYAVDDMLLKRLRMGKP
                     WEHASNLGGILADAICRSRNLPAFIVDPVAVDEMNPVAKITGLPELPKASLGHALNIK
                     ATVRRAAGDLGKAWDELNFIVAHIGGGITVCAHEKGRMTDLNNANEFGPFSPERAGGL
                     PAGDLVRMCFGESMTEKDMLRKMVGRGGLMAYTGTSDVREVKTMAAGGDERAREALDA
                     MAYAVAKEIGAQAAVLSGNVDAVLFTGGVAFDSDFVAAVQKRVQWIAPVLVYPGEDEM
                     PALAEGALRVLRGEEECKIYGRYISGGEKA"
     gene            complement(1624..>2837)
                     /locus_tag="P5219_04230"
     CDS             complement(1624..>2837)
                     /locus_tag="P5219_04230"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRX25995.1"
                     /translation="DETFTGFAIYRDISERKKAEDEMLKNLKREALISSVSARLLEEG
                     VRGGVPPSLEELAAFFGVSRGCLVETDGDGIPVREFSLSEGRVEFLSSPEVSAVLSPE
                     ALKGLNLYLWETGKLVLESSPRPGGKEVPRFAGELLELWGDPLHFQPVYSGGAVRGFL
                     VLRLAGVPLHHSDEALLAMYCDLAGSAFLRDRRINIMEEAGRMLDSASRGIVEILGQA
                     LAMKDPYTVGHQINVAGLARAMAKKGGYDEAFQERVYYAGLVHDLGKITIPSAILSKP
                     GRLTDIEFSLIRNHALYGWDILSSVDLPWPLADIVVQHHERLDGSGYPKGLRGDAIGR
                     EARFLSVADVVEAMTSHRPYRPGLGIVAALDEIRSHRGGWFDPEAVDLCIAVIEEGFT
                     FDGGSYRLFLP"
BASE COUNT          524 a          907 c          856 g          550 t
ORIGIN      
        1 ccgtcggaga tgccgatgac ccggcggttc attgcgggaa tctcgaagag gacgagatgg
       61 gaaagaaggg atcctgtccg gaggccccat tccttgtcca ggaccgcctt gagaagggtc
      121 gatgtcttca cgagcccctt catgagcaga tccgcctcgc ccgaggatac catcctgacg
      181 ctccgctcca ccgccttctc gtcgtccttt tcgtcaacga tggtgacccc ggagaggtcg
      241 cacccgtcct gcccggcgag ggtacggatc cttgcggcgt caccgacgag caccgccttg
      301 accactccct cacggcctgc gcgggtgacg gcggcgaggg catcgtcacc gtagggacag
      361 gcaagagcga gggtcatggg gcgtcccttc ctgctttcct caaggagaaa atccagtttc
      421 tgcatggtca tgccttttcg cctcccgaaa tataccggcc atagattttg cattcttctt
      481 caccgcgaag gacccggagc gctccctccg cgagagcggg catttcgtcc tcgccgggat
      541 aaaccagcac cggagctatc cactggaccc gcttttgaac ggcagccacg aagtcgctgt
      601 cgaaggccac tcctcccgta aagagaacgg cgtccacatt gccggacaga accgcggcct
      661 gggcgccgat ctccttcgcg acggcgtagg ccatggcgtc cagggcttcc cgtgcccgct
      721 catctcctcc ggccgccatg gttttcactt ccctgacgtc cgacgttccc gtgtaggcca
      781 taaggcctcc cctgccgacc atcttccgga gcatatcctt ttcggtcatg ctctcgccga
      841 agcacatccg cacaaggtct ccggcgggaa gtcctccggc cctctccggg gagaaggggc
      901 cgaactcgtt ggcgttgttc aggtcggtca tccgtccctt ttcgtgggcg cagaccgtga
      961 tgcctccgcc gatgtgggcc acgatgaagt tgagttcgtc ccaggccttc ccgagatctc
     1021 cggcggccct ccggaccgta gccttgatgt tgagggcatg acccagggag gctttgggaa
     1081 gctcggggag cccggtgatc ttggccaccg ggttcatctc atccaccgcc acggggtcca
     1141 cgatgaaggc ggggaggttc cgggatcggc agatggcgtc ggcgagtatg cctccgaggt
     1201 tggaggcgtg ctcccagggc ttgcccatgc ggagacgctt cagcagcatg tcgtccacgg
     1261 cgtaggttcc cccggggatg ggctccacga gcccgccccg ccccgccacg gcggaaagtt
     1321 cgtcgaagga tgccccgtgg gaggccagag cctccgctat ggtctccagg cgaaaggaaa
     1381 actggtcggc caccgaggga aaggcggcga gggcgtccgg atcgtgcctg accgtctcac
     1441 gccaggtctc agtgccgtcg gaataccacg cgatctttgt gcttgtggaa ccgggattga
     1501 ttgcaaggat ctgaaaggcc atggctcgtt cctcccctgt gtgcagcgga tcctgtctat
     1561 agttttctga ttccatatgt cgaaactgaa catattatac agtatatcct ttttccggaa
     1621 cacttatgga aggaaaagac ggtaacttcc tccgtcaaag gtgaatcctt cctcgatgac
     1681 ggcaatgcag aggtccaccg cctccggatc gaaccagccc ccccggtggg atcgtatttc
     1741 gtcaagggcc gcaacgattc cgagtccggg ccggtacggc ctgtgggagg tcatggcttc
     1801 caccacgtca gcaacggaga ggaagcgggc ctcccggccg atggcgtccc ccctgagccc
     1861 cttcgggtac cccgaaccgt caagccgttc gtggtgctgt accacgatgt ctgccagggg
     1921 ccagggcaga tcgacggagg agaggatgtc ccacccgtag agggcgtggt tccttatcag
     1981 ggaaaattcg atgtcggtga gccttcccgg cttggagaga atggccgagg gaatggtgat
     2041 ctttccgagg tcgtggacga gcccggcgta gtagaccctt tcctggaagg cctcgtcgta
     2101 ccctcccttt ttcgccatgg ccctcgcaag gccggccacg ttgatctggt gccccaccgt
     2161 gtagggatcc ttcatggcca gggcttgtcc gagaatctcc acgatgcccc tggaagcgct
     2221 gtccagcatc ctgccggcct cttccatgat gtttatccgc cggtcccgca gaaaggcgct
     2281 tcccgcgaga tcgcagtaca ttgcaagaag ggcttcgtcg ctgtggtgca gggggacacc
     2341 ggcgagccgg aggacaagga atcccctcac cgctcccccg gaatacacag gctgaaagtg
     2401 cagcgggtcg ccccaaagct ccagaagctc tccggcaaag cggggaacct ccttcccgcc
     2461 gggacggggg gacgattcaa ggaccagttt ccccgtttcc caaaggtaca ggttcagtcc
     2521 cttgagcgcc tccggggaaa gcacagcgga aacttcggga gaagacagga attccaccct
     2581 tccttcggaa agggagaatt cccggacggg gatcccgtcg ccgtcggtct ccacaaggca
     2641 tccccgggaa acgccgaaaa aggccgccag ctcctccagg gacgggggaa cccctccccg
     2701 gaccccctcc tcaaggagcc gggcggaaac agaggatatg agagcctctc tcttcaggtt
     2761 cttcagcatc tcgtcctcgg cttttttccg ttccgagatg tcccggtaaa tggcgaagcc
     2821 ggtaaaggtt tcatccc
//
LOCUS       DAOTHD010000185         2836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_41872_length_2836_cov_3.664869, whole genome shotgun sequence.
ACCESSION   DAOTHD010000185 DAOTHD010000000
VERSION     DAOTHD010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41872_length_2836_cov_3.664869"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..741
                     /gene="rimP"
                     /locus_tag="P5219_04235"
     CDS             226..741
                     /gene="rimP"
                     /locus_tag="P5219_04235"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HRX25996.1"
                     /translation="MGEDREKENFRALREIVEGLGYECVGITLGNEERRAILRVYIDS
                     LGGILVKDCETVSRSLNRFLDENEDYVRGQFYLEVSSPGIERPLFSLEDYRKFSGKKI
                     KVRTRQEIVGQKRFTGLLLGAGEDGTVLLRLETGRGSDEDETVRIPFETITKGNLVYE
                     EQDKQDKRRSS"
     gene            742..1845
                     /gene="nusA"
                     /locus_tag="P5219_04240"
     CDS             742..1845
                     /gene="nusA"
                     /locus_tag="P5219_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRX25997.1"
                     /translation="MQLGRDFVRALAQITKERGLSQEVIASSLEAALISAYKKYQGGN
                     QDVEVHIDTENGDISICEVKHIVADVVSPDTEISLADAQAQGFVDVTEGDFIRIEKNP
                     ENFGRIAAQTARQVIIQRLKDAERQIIFEEFSDRVGDLVTGVVFKSENDQVLIRLNDR
                     TEAILPREERIVNEKYIPGERLKFYLLDVRQTTRGPRIVVSRTHPGLLRKLLELEVPE
                     IQEGVIEIKNIVREAGARAKVAVQTLDMNVDPVGACVGNNGARIKSISKELCGEKVDV
                     IIWSGDPLQYIRNSLSPAKIVKIEPILEQEKAVTVFSRPDQLSLAIGKAGQNVRLAAR
                     LTGWKIDINALEPERMPTLHDIFQDIIDDSGAE"
     gene            1856..2137
                     /locus_tag="P5219_04245"
     CDS             1856..2137
                     /locus_tag="P5219_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HRX25998.1"
                     /translation="MMRRRPRTCVGCREESPKREMLRIARNAAGEVSVDPSGRLPGRG
                     AYVCLKEECILSARKRDALSKALRVKVDAGIYDALLKMASEAENGDKGQ"
     gene            2134..2472
                     /locus_tag="P5219_04250"
     CDS             2134..2472
                     /locus_tag="P5219_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX25999.1"
                     /translation="MKKRKEALLSVFGMARRANILQIGQDIVKSSLARGEALLVLFAG
                     EGESSFYRSLQNGRFAEKSTITVLPDISGSELSAAIGSGNVKVVALPLRSGFARSISQ
                     LLAEGGDVNE"
     gene            2465..>2836
                     /locus_tag="P5219_04255"
     CDS             2465..>2836
                     /locus_tag="P5219_04255"
                     /inference="COORDINATES: protein motif:HMM:NF016635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HRX26000.1"
                     /translation="MSKIRVYELAKLLGKSNKELLDILIDLGVDVKSHMSSIDNDIAQ
                     LVEESLGGASVTQGPGKKEEERSVIKTDSFEVSRGSTVKAVAQLIGISPAEAVKCLIS
                     AGLMVPADSPVDEKSLKVLQDA"
BASE COUNT          704 a          687 c          862 g          583 t
ORIGIN      
        1 gatagccgag gattttttga cagcggacga tcccatggat ttcgaggcga atctggacga
       61 gtggtggccg gagttcatct cggacttttg tctgtagcga aagggaagtt gtatttattc
      121 ccaataatgt gctatactcc aatgtggtgt ttcttcggag agtcttttca aagagtgggt
      181 tcccccactc tttgtttttt aataggcctg cagggagaga tgggcatggg tgaagacagg
      241 gaaaaggaaa atttcagagc cctccgggaa atagttgagg ggctcggtta cgaatgtgtg
      301 ggcatcaccc tcggcaacga ggagcggcgg gcaattctga gggtatatat agattctctc
      361 ggagggatac tggtcaagga ctgcgagacg gtctccaggt cactaaaccg tttccttgat
      421 gaaaatgagg actatgtccg cggacagttc tacctggagg tgagttctcc gggaatcgaa
      481 cgtcctcttt tttcccttga agattaccgg aaattttcgg gaaagaaaat aaaggtcagg
      541 accaggcagg aaatcgtcgg acagaaacgt tttacgggcc tccttctggg cgcgggagag
      601 gacgggactg ttcttctccg gcttgagaca ggcagaggat cggatgagga tgaaacggtc
      661 cggattccct ttgaaacaat taccaagggc aaccttgtgt atgaggaaca ggacaagcag
      721 gacaaaagga ggagttcata aatgcagctt gggcgtgatt tcgtccgcgc tttggcccag
      781 atcacgaagg agaggggact gtcccaggag gttatcgctt ccagcctgga ggcggccctt
      841 atctcggcat ataaaaagta ccagggcggg aaccaggatg tggaggtcca tatcgatacg
      901 gagaacggcg acatctccat ctgtgaagta aagcacatcg tggcggatgt cgtttctccg
      961 gatacggaaa tctcccttgc cgacgcccag gcccaggggt tcgtggacgt gacggagggt
     1021 gatttcatcc gcatcgagaa aaaccccgag aatttcggaa gaattgcggc ccagacggcc
     1081 cggcaggtca tcatccagag gctgaaggat gccgaacggc agatcatttt cgaggagttc
     1141 tccgaccggg tgggggatct cgtcacgggc gtcgttttca aatcggagaa tgaccaggtg
     1201 ctgatccgtc tcaacgaccg gacggaagcg atcctcccca gggaagagcg tatcgtgaac
     1261 gaaaagtaca ttcccggtga aaggctcaag ttttacctcc ttgacgtgcg ccagacaaca
     1321 cggggcccca ggatcgtcgt ttcccgcacc catccggggc tgctgagaaa gcttcttgaa
     1381 ctcgaggttc ccgagatcca ggaaggggtc atcgagatca aaaacatcgt ccgcgaggca
     1441 ggagccagag ccaaggtggc ggtccagacc cttgacatga acgtggatcc cgtgggtgcc
     1501 tgcgtgggga acaatggggc caggatcaag tccatcagca aggaactctg cggcgaaaag
     1561 gtggacgtga tcatctggag cggcgatccc ctccagtaca tccggaactc cctctctccg
     1621 gcgaagatcg tcaagatcga acccattctt gagcaggaaa aggcagttac cgtcttttcc
     1681 cgtcccgacc agctctccct cgccatcggc aaggccggac agaatgtccg cctggctgcg
     1741 aggctcacgg gatggaagat tgacatcaat gccctggaac cggagagaat gcccacgctc
     1801 catgacattt tccaggacat catagacgac agcggtgccg aatagaggga cggccatgat
     1861 gcggcggcgg ccgaggacct gcgtcgggtg cagggaggaa tctcccaaga gggaaatgct
     1921 gagaatagcc cgcaacgcgg cgggagaggt gtccgtcgac ccttcgggaa ggcttcccgg
     1981 aaggggagca tacgtgtgtc tcaaggaaga atgcattctc agcgcccgga aaagggatgc
     2041 cctttcaaaa gctctcagag tgaaagttga cgccgggata tacgacgctc tcctgaaaat
     2101 ggcttccgaa gctgaaaacg gggacaaagg gcagtgaaaa aacgaaagga agccctgctc
     2161 tcggttttcg gcatggcgcg aagagcgaac atcctgcaaa ttggtcagga tatagtaaaa
     2221 tcttctttgg cccggggtga agcgctcttg gtgcttttcg caggagaagg ggaatcgtct
     2281 ttctaccggt ccctccagaa tgggaggttt gcagaaaaaa gcacgatcac tgttctgccg
     2341 gatatttccg gaagcgaact ctctgcggct atcggttcag gaaatgtgaa ggttgttgct
     2401 ttaccgttac gcagcggctt tgcacgttct atctcgcaac tgcttgcgga agggggagac
     2461 gtaaatgagt aaaatccggg tatatgaact ggccaaactg ctcggcaaaa gtaacaagga
     2521 actgctggac attctgatcg acctcggggt cgacgtaaag agtcacatga gttccattga
     2581 caacgatatc gcccagcttg tggaggaatc cctcggagga gcctccgtta cgcaagggcc
     2641 tggaaaaaag gaagaggagc gcagcgtgat aaaaacagat tctttcgaag tttcaagggg
     2701 atccacagtc aaggcggttg cccagctcat cggcatctct cctgcggaag cggtgaagtg
     2761 ccttatctcc gccggcctga tggtaccggc ggacagcccg gtggacgaaa agagcctgaa
     2821 ggttctgcag gacgcc
//
LOCUS       DAOTHD010000186         2833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_41945_length_2833_cov_3.828654, whole genome shotgun sequence.
ACCESSION   DAOTHD010000186 DAOTHD010000000
VERSION     DAOTHD010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41945_length_2833_cov_3.828654"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..1230
                     /gene="dctP"
                     /locus_tag="P5219_04260"
     CDS             181..1230
                     /gene="dctP"
                     /locus_tag="P5219_04260"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRX26001.1"
                     /translation="MKRKLIRAGLMALFCISVFSGASFAKDIVLRLSDHDVLGGLKGQ
                     IGTFWFNEIEKQTGGKVKIKAYWGGTLFSAEEQLKALSDGVVDIAAIYPDFYPKQLPL
                     FGAFWLFPKGPENWENIRWIYKESIAKIPEFDRELKDAGVKILLMENGLPMSYMARYP
                     ITSLKDIKGKKWRAGDRWNLKLLENQGAVAISVPWGDCYMALETGTVDGIVSNYDGMH
                     RQKFDEPAKNILAGKAIGWSQPMFYCINQEKWDSIPKDLQEGILKATEITEKAFGDMY
                     NGEFNRIVEAEKKAGCTVNFYSPEDVDSWADEKCLEGLREIFCTELQDDGIENSREML
                     KQMKAIIDEGIAKEK"
     gene            1392..1877
                     /locus_tag="P5219_04265"
     CDS             1392..1877
                     /locus_tag="P5219_04265"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRX26002.1"
                     /translation="MQKILTKINRMTAELGGLLMTVMMVLFLVNIISREMNRPIQGLL
                     QMAVFSMVILIYLGLAHCEEKDEHVRLEVVMTRLPVKMRTRLRLACTVIELLIVTLLL
                     YAGARDALSAFVTGASVTGTKPMLLWPVKTVMVAGLVLFWLQLLARSLSFLKEMRSNG
                     A"
     gene            1932..>2833
                     /locus_tag="P5219_04270"
     CDS             1932..>2833
                     /locus_tag="P5219_04270"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRX26003.1"
                     /translation="MEPVTVGIIGIVALLASLGMGVSIGLAFILVSFATVLLLMGFDP
                     ALSFLGQTMYHSIASPNWCSIPLFILMGAFAAVAGFGKAIYDGLYKLTRRWPGSLAVA
                     TCFGSALFGAISGSSLANTAIFGKLVLPEMDRYKYDRSFAAACVASAGTFANMIPPSI
                     NFVIFALFTEQSIARLFAAGVIPGIFTALVYAASIFIRVRRNPHLAPEAEIHEKLSFR
                     EKMECYKKMMPIGIVILVTFGGIYGGIFSPTEAAAAGCLITLIVGWFQGSIRKIGDLR
                     EPLRESANTTAMLFIINVGALFFSR"
BASE COUNT          623 a          762 c          831 g          617 t
ORIGIN      
        1 gaaggaggag acttccgcag gttcttttgc cgggacattc tgatcctttc gccgggcatg
       61 ctgcctgaac gggggcgttt cccggcggaa aagcgaggtg attcgatcgg acaaaaatgg
      121 gttccgggaa cgcagagagt tgaaggggtg ttcgtctatt ttttcaaggg aggtcatgaa
      181 gtgaaaagaa aactgatcag ggcaggactt atggcacttt tttgcatttc cgttttcagc
      241 ggcgcatcgt ttgcgaagga catcgtgctg aggctgtcgg accacgatgt tctcggaggg
      301 ctgaagggac agatcgggac cttctggttc aatgagatcg agaagcagac cggcggaaag
      361 gtcaagatca aggcctactg gggcggcact cttttcagcg cggaagagca gctcaaggct
      421 ctttccgacg gagtcgtcga catcgccgcc atttatcccg acttctatcc gaagcagctt
      481 ccccttttcg gcgcattctg gctcttcccg aaaggaccgg aaaactggga aaacatcagg
      541 tggatctata aagagagcat cgcgaaaatc cccgaattcg acagggaact gaaagatgcc
      601 ggagtcaaga tactgctcat ggagaacggc ctccccatgt cctacatggc ccgctacccc
      661 atcacgtccc tgaaggacat aaaaggcaaa aagtggaggg ccggcgaccg ctggaacctg
      721 aagctgctcg agaaccaggg agccgtggcc atttccgtgc cctggggcga ctgctacatg
      781 gccctggaaa cgggaaccgt ggacggcatc gtgtccaatt atgacggcat gcacaggcag
      841 aagttcgacg agccggcgaa gaatatcctt gccggaaagg ccatcggctg gtcccagccc
      901 atgttctact gcatcaacca ggaaaaatgg gacagcattc ccaaggatct ccaggaaggg
      961 atcctcaagg ctaccgaaat aacggaaaag gccttcggcg acatgtacaa cggcgagttc
     1021 aacaggatcg tggaggcgga gaaaaaggcc ggctgcaccg tgaatttcta ttcccccgaa
     1081 gacgtggaca gctgggcgga tgaaaaatgc ctcgaagggc tccgggaaat tttctgcacg
     1141 gagctgcagg acgacggcat agagaattcc cgggaaatgc tgaagcagat gaaggccatt
     1201 atcgacgaag gaattgcgaa ggagaaataa agagagcctg ccggccgcgg aatggtagaa
     1261 aacaccccca tgccgcggcc ggttcttttt ttgctcgcgg ctcatcctgg tttcatccag
     1321 ctttcaaatc cgttcgcgct cttcccggcc cggttttctc cgggaaaagc gccctggcaa
     1381 ggagtatgag aatgcagaag atattgacga aaatcaaccg tatgacggcc gaactcggcg
     1441 gattgctgat gacggtgatg atggtgcttt ttctcgtgaa tatcatttcg agagagatga
     1501 acaggccgat ccaggggctg ctccagatgg ccgtattttc catggtcata ttgatttatc
     1561 tcgggttggc ccactgcgag gaaaaagacg agcatgtccg gctggaggtc gtcatgacga
     1621 ggcttccggt gaaaatgaga acgaggctcc ggctggcgtg caccgtcatt gaacttttga
     1681 tagtgaccct tcttctctac gccggggcac gggacgccct gtcggccttc gtcaccgggg
     1741 ccagcgttac gggaacgaag cccatgctgc tgtggcccgt gaagacggtc atggtggcgg
     1801 ggctggtgct cttctggctc cagcttctcg ccaggagcct gtctttcctg aaggaaatgc
     1861 gaagcaatgg ggcgtaaccg tcttccgggg aaaagcggaa tacaaaaact actacgtatg
     1921 gagtgaagtg gatggaaccc gtaacggtag gcatcatcgg tattgtggcc cttctcgctt
     1981 ccctgggaat gggcgtttcc atcggcctgg cctttattct ggtcagtttc gccactgtgc
     2041 tgctgctcat gggcttcgac ccggcccttt ccttcctcgg ccagacaatg taccattcca
     2101 tcgccagccc gaactggtgc tccattcccc tcttcatcct catgggggcc tttgcggctg
     2161 tggcgggttt cgggaaggcc atatacgacg ggctgtataa gctgacacgg cggtggccgg
     2221 ggtccctcgc cgttgccacc tgcttcggct ccgccctctt cggcgccatt tccggttcgt
     2281 ccctcgcgaa cacggcgatt ttcgggaagc tggtcctgcc ggaaatggac aggtacaagt
     2341 acgacaggtc cttcgcggcg gcatgtgtgg catctgcggg taccttcgcc aacatgattc
     2401 ctcccagcat caattttgtc attttcgccc tttttaccga acagtccata gcgcggcttt
     2461 ttgcggcggg ggtcattccc gggattttta cggccctggt ctatgccgct tccattttca
     2521 tccgggtcag gagaaatccc catctcgccc ccgaggcgga gatccacgaa aaactgtcct
     2581 tcagggaaaa gatggagtgc tacaaaaaaa tgatgcccat cggcatcgtt atcctcgtga
     2641 ccttcggggg catctacggc gggattttca gccccacgga ggcggcggcg gcagggtgcc
     2701 tgataaccct catcgtcggg tggttccagg gatccatcag gaaaatagga gatctccgcg
     2761 aaccgctccg ggaatcggcc aataccacgg caatgctctt catcatcaac gtgggggccc
     2821 ttttcttttc ccg
//
LOCUS       DAOTHD010000187         2832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_41984_length_2832_cov_2.658985, whole genome shotgun sequence.
ACCESSION   DAOTHD010000187 DAOTHD010000000
VERSION     DAOTHD010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41984_length_2832_cov_2.658985"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="P5219_04275"
     CDS             <1..919
                     /locus_tag="P5219_04275"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRX26004.1"
                     /translation="GKNPDYLRDIPLPAGVSATSGIEEAALFSHYWILALPTQTLREF
                     LPGLGAYCTGRTEICNVAKGIEIDTGKRISEIVREILPVGLYSVLSGPSFAEEVARGL
                     PTAVTVASGNEGSALFWQSLLNTSRLRIYTSHDVAGTETGGAVKNIMAIASGLASSLG
                     LGDNARAALVSRGLAEIMRFGEAIGAHPLTLAGLAGMGDLVLTCYSTQSRNFRLGMAL
                     GRGLSLEEARKEIGQVAEGAYTVRAVTEAAASLSVDMPISRGVHRLLYEGASPAEELE
                     RLLTRDPKAEYPPAILWGSSSCPEKESGV"
     gene            970..1140
                     /locus_tag="P5219_04280"
     CDS             970..1140
                     /locus_tag="P5219_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRX26005.1"
                     /translation="MAKAVVDKDACVGCETCVGTCPVEAISMVDGKALVDESCIECGS
                     CVSVCPVNAITQ"
     gene            1265..2323
                     /locus_tag="P5219_04285"
     CDS             1265..2323
                     /locus_tag="P5219_04285"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRX26006.1"
                     /translation="MAYRTVHRGNRLERELPALRLLSVITAVWFLAAGWADVLMGYHL
                     LAVLDFFLASLFLFFRLRMNAMPISAVRGFVPPILLFLNLLPLGASFLEAGFFTLLLR
                     HIVTLLGTWVFMQFRRRDVFFTYLVINAFSFLGTFLLAGDFRGETANLAICYIFIVSS
                     MYFFSMLQRKTSRELAALSTAERQARDMASRDPLTGLFNRRHFDENLARMLREGTGVT
                     LLILDLDDFKDINDVHGHQAGDEALKKMAAAVSRAVRRDDIACRIGGDEFAVLLPACP
                     ENNGLVIAENICRQLRSCAPYLSVSLGVAFAPGGSSPEDAFRKADAALYEAKSKGKGC
                     IVSGAMLTPAFASLCGKD"
     gene            2401..>2832
                     /locus_tag="P5219_04290"
     CDS             2401..>2832
                     /locus_tag="P5219_04290"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRX26007.1"
                     /translation="MDRLILSLLIINFAIYSYSNAFFFLAPYLAGRGYSAASAGILVG
                     VFYAATTAVRPVGSWITERIGVRKSMIWSSGVCIACALALQLYQGAFGWFVGVRVVMG
                     LAYSIFMVALTTYQALVIEESKRGLGFVLVSVGSLIPMFTVV"
BASE COUNT          533 a          895 c          829 g          575 t
ORIGIN      
        1 ggggaaaaac ccggactatc tccgggacat ccccctccct gcaggagtat cggcgacatc
       61 aggcatcgag gaggcggccc tgttttcaca ctactggata ctggccctcc ccacccagac
      121 gctcagggaa tttctccccg ggctgggagc atactgcacc ggccggacgg agatctgcaa
      181 cgtggccaag ggcatagaga tcgacacggg aaaacggata agcgagatcg tgagggagat
      241 ccttcccgtc gggctctatt cggtcctctc cgggccgagt ttcgctgaag aggtcgccag
      301 ggggcttccc acggcggtga ccgtcgcctc cgggaacgaa gggtccgctc ttttctggca
      361 gtccctgctc aacacgtccc ggctcaggat ctatacatcc catgacgttg ccggaactga
      421 gaccgggggg gcggtgaaaa acatcatggc catcgcctcc gggctggcct cgtccctcgg
      481 cctgggggac aatgcccggg cggctctggt gagcaggggg ctggcggaga tcatgaggtt
      541 cggcgaggcc atcggcgccc atcctctcac actcgcgggc cttgctggaa tgggagacct
      601 ggttctcacc tgctacagca cccagtcgcg gaatttcagg ctcggcatgg ccctcggacg
      661 gggtctttcc ctggaagagg cccggaagga gatcggccag gtggccgagg gggcctatac
      721 cgtacgggcg gtgaccgaag cggctgcaag cctttcagtg gacatgccca tctccagggg
      781 ggtccaccgc ctgctgtatg aaggcgcttc cccggcggaa gaactggaac ggctgctcac
      841 cagggaccca aaagcggaat accctcccgc catactctgg ggctcttcaa gttgcccgga
      901 aaaagaaagt ggggtataat ccggaccggc acagtgtacc agcagactat atccgaggag
      961 gaatgaacaa tggcaaaagc agtcgtggac aaggatgcat gcgtaggttg cgagacctgt
     1021 gtcggcacct gccccgtgga agccatttcc atggttgacg gcaaggcgct tgtggacgag
     1081 agctgcatcg agtgcggcag ctgcgtgtcc gtctgccccg tgaacgcgat tacccagtaa
     1141 tccgctttcg gaaaaggctg aatccgccct tttccggccc aggccgggaa agggctcttt
     1201 ttttcccgcc tcatggaggg tataatcctt gtcgcaggca gtcaacaaag tccgggaggc
     1261 tgcaatggca taccgcaccg ttcacagggg aaaccgactc gaaagagaat tgcccgccct
     1321 ccgtctcctc agcgtcatca cagcggtctg gtttctcgcc gcaggctggg ccgacgtgct
     1381 gatgggatat catctcctgg cggtgctgga ctttttcctc gcctcccttt tcctgttttt
     1441 ccgcctcagg atgaacgcca tgcccatttc ggccgtccgg ggattcgttc cgcccatcct
     1501 gctcttcctg aacctgctgc ccctgggggc ctccttcctc gaagcgggtt tcttcactct
     1561 tcttctccgg catatcgtca cccttctggg gacatgggtt ttcatgcagt tccggcgacg
     1621 ggacgtcttc ttcacctatc tcgtgatcaa cgcattttcc ttcctcggca cctttctcct
     1681 ggccggggac ttccggggag aaacagcaaa cctggccatc tgctatattt tcatcgtctc
     1741 cagcatgtac tttttctcca tgctgcaaag aaagacctcc agggaactcg ccgccctgtc
     1801 aaccgccgag cggcaggcac gggacatggc cagcagggat cctctcacgg gactcttcaa
     1861 ccggcgccat ttcgacgaaa accttgcccg gatgctccgg gagggaaccg gagtcaccct
     1921 ccttattctc gacctcgacg atttcaagga catcaacgac gtccacggac accaggcggg
     1981 ggatgaagcc ctgaaaaaaa tggctgcagc cgtctcccgg gccgtccgga gagacgacat
     2041 cgcctgcagg ataggggggg acgaatttgc cgtccttctc cccgcctgcc cggagaacaa
     2101 cggcctggtg atcgcggaaa atatctgccg ccagcttcga agctgcgctc cgtacctgag
     2161 cgtgtccctc ggcgtggcgt tcgctccggg cggttcgtcc ccggaggatg ccttcagaaa
     2221 ggccgacgcg gccctctacg aggcgaaatc gaagggcaag ggatgcatcg tatccggagc
     2281 tatgctcacc cccgcatttg cctcactctg cggaaaggat tgaaccaact cccccgggag
     2341 catacaatac acccgtccgg aaccgccccg gaccataacg gcaccgagga ggcctccaga
     2401 atggacaggc ttattctgtc tctcttgatt atcaatttcg caatctattc ctattccaac
     2461 gcattcttct tcctcgcccc ctaccttgcc gggaggggat actccgcggc gtcggcggga
     2521 atcctcgtcg gcgtcttcta tgccgccacc acggcggtcc ggccggtggg aagctggatc
     2581 accgaacgga tcggcgtgag aaagagcatg atctggtcct ccggcgtctg catcgcctgc
     2641 gccctggccc tccaactcta ccagggtgcc ttcggctggt tcgtcggagt gagggtggtc
     2701 atggggttgg catacagcat tttcatggtg gccctcacca cctaccaggc cctcgtcatc
     2761 gaagaatcga agcgggggct cggattcgtc ctcgtctccg tgggaagcct catccccatg
     2821 ttcaccgtgg tc
//
LOCUS       DAOTHD010000188         2819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_42253_length_2819_cov_3.374457, whole genome shotgun sequence.
ACCESSION   DAOTHD010000188 DAOTHD010000000
VERSION     DAOTHD010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42253_length_2819_cov_3.374457"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1893)
                     /gene="secA"
                     /locus_tag="P5219_04295"
     CDS             complement(<1..1893)
                     /gene="secA"
                     /locus_tag="P5219_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRX26008.1"
                     /translation="MLDKLKKALGLDPNDRALKRYREKAEEINRLEPETAALTDEELR
                     SRGPEFRRRLENGESPDDLLPEVFAVVREVSKRTIGLRHFDVQLMGGMALHEGKITEM
                     KTGEGKTLVATLAVVLNALSGKGVHVVTVNDYLARRDAEWMGPIYSFLGLTVGVIYAY
                     MDQEERFAAYRADITYGTNSEFGFDYLRDNMATSEEYLVQRGHSYCIVDEVDSILIDE
                     ARTPLIISGPSDDNVDMYTLSDKVARQLREGRDYEKDEKERNVAMTEAGIARCEEILK
                     MPELFSDAAHSDLAHRIVQALKAHVLFQKDVHYVVKDGEILIVDEFTGRLMVGRRYSD
                     GLHQAIEAKEGVKVGRESQTLATITLQNYFRMYRKLAGMTGTAATEAEEFKEIYGLEV
                     IVVPTNVDMIRNDNPDAIYRTVTEKFAAVADEIQESHTQGCPVLVGTTSIENSERISK
                     LLKARKIPHQVLNAKHHEREAQIVAQAGHLGAVTVATNMAGRGTDILLGGNPEFLARE
                     ALRSQNPDPSKEQEMHVSLLAKFREQCRAERDRVKELGGLKIIGTERHEARRIDNQLR
                     GRAGRQGDPGSSRFYLSLEDDLLRLFGSERIQGLMEKLGMEEGESIEHPLLTKAIASA
                     QKKVEEM"
     gene            complement(2022..2561)
                     /gene="pgsA"
                     /locus_tag="P5219_04300"
     CDS             complement(2022..2561)
                     /gene="pgsA"
                     /locus_tag="P5219_04300"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HRX26009.1"
                     /translation="MLSLLRVFLAPLVLIFLTFQFTYPVYAVEELELRVTVGDILAGT
                     VFILAALTDTADGYIARKKGMVTNLGKFIDPVADKVLVMAALISLVELQRIPAWIVVV
                     ILSRDLIVTGVRMVAAAEGVVIAASRGGKVKTVFQIVAISMLIFNLPFGIQVMWIAMI
                     LTVWSGMDYLVKGKKLFTD"
BASE COUNT          485 a          857 c          860 g          617 t
ORIGIN      
        1 catctcctcc acctttttct gggcggaggc gatggccttg gtgagaaggg ggtgctctat
       61 ggactcccct tcctccatgc cgagtttctc catgagcccc tggatcctct cggagccgaa
      121 gagtcgaagg aggtcgtcct ccagggaaag gtagaatcgg ctcgaaccgg ggtcgccctg
      181 gcgccccgcc cgtccgcgga gctggttgtc gatgcgccgg gcctcgtggc gttcggtgcc
      241 gatgattttc aggccgccga gctcctttac cctgtcccgt tccgcacggc actgctcacg
      301 gaatttcgca aggagggaga cgtgcatttc ctgttctttc gagggatcgg gattctggga
      361 ccggagggct tccctggcaa ggaactccgg attgccgccg agaagaatgt ccgttccgcg
      421 gccggccatg ttggtggcca ccgtgacggc acccagatgc cccgcctggg cgacgatctg
      481 ggcttcccgc tcgtggtgct tggcgttcag cacctggtgg gggatcttcc gtgccttcag
      541 aagtttgctg atcctctcgg agttttcgat ggaggtggtt cccacgagaa ccgggcagcc
      601 ctgggtgtgg gactcctgta tctcgtcggc cacggcggcg aatttttccg tcacggtgcg
      661 gtagatggcg tcagggttgt cgttcctgat catgtccacg ttggtgggga ccacgatgac
      721 ttcgaggccg tagatctcct tgaactcctc ggcctctgtg gcggcggttc ccgtcatgcc
      781 cgccagcttg cggtacatgc ggaagtaatt ctgcagagtg atggtggcca gtgtctggct
      841 ctccctgccc accttgaccc cttccttcgc ctcgatggcc tggtggagcc cgtcggagta
      901 ccgtcgtccc accatgagcc tgcccgtgaa ttcgtcgacg atgaggatct ccccgtcctt
      961 caccacgtag tgtacgtcct tctggaagag aacgtgggcc ttgagggcct ggacgattct
     1021 gtgggcgaga tccgagtggg cggcatcgga gaagagctcc ggcatcttca ggatctcctc
     1081 gcacctggcg atgcccgctt ccgtcatggc cacgttccgt tccttctcgt ccttctcgta
     1141 gtcccgtccc tcccggagct gccgggctac cttgtcggac agggtgtaca tgtccacgtt
     1201 gtcgtccgag ggaccggaaa tgatcagggg ggtcctcgcc tcgtcgatga ggatggagtc
     1261 cacctcgtcc acgatgcagt aggaatgtcc ccgctggacg aggtactcct ccgaggtggc
     1321 catgttgtcc ctcaggtagt cgaagccgaa ctcgctgttc gttccgtagg taatgtcggc
     1381 ccggtaggcg gcgaaccgct cctcctggtc catgtaggcg tagataaccc ccaccgtgag
     1441 accgaggaag gagtagatgg ggcccatcca ttccgcgtcc cgccgggcga ggtagtcgtt
     1501 caccgtcacc acgtggacgc ctttgccgga aagggcgttc agcaccacgg caagggtggc
     1561 caccagcgtc ttgccttcgc cggtcttcat ctccgtgatt tttccctcgt ggagggccat
     1621 tccgcccatg agctggacat cgaagtggcg aagcccgatg gtcctcttcg agacctcccg
     1681 cacgacggcg aacacctcgg gaagaagatc gtcgggggat tccccgttct caaggcgccg
     1741 tctgaactcc ggccccctgc tcctgagttc ctcgtccgtc agggccgccg tttccggctc
     1801 gaggcggttg atctcctccg ccttttccct gtagcgtttc agcgcccggt cgttgggatc
     1861 gaggcccagg gctttcttca gtttatcgag catgacgtca cctcaaaaga aagatcagcc
     1921 gtggttcggg attcttttcc acggcggttt ccacccggac attatatgct ctgggagtgc
     1981 atggggtcaa agtatttcgg cggaaaaggg aaggcgggcg ttcagtcggt gaagagcttc
     2041 tttcccttga cgaggtagtc catgccggac cacacggtga ggatcatggc gatccacatg
     2101 acctggattc cgaagggaag gttgaaaata agcatggaga tggccacgat ctggaacacc
     2161 gtcttgacct tccctccccg tgaagctgcg atgaccaccc cttcggcggc ggcgaccatg
     2221 cgcaccccgg tgacaatgag atcccgggaa aggatcacca ccacgatcca ggccggaatg
     2281 cgctgcagct ccaccagcga gatcagggcc gccatgacga gaaccttgtc ggccaccggg
     2341 tcgatgaact tgcccaggtt ggtgaccatg ccctttttcc tggcgatgta gccgtcggcg
     2401 gtatcggtga gggcagcgag gatgaagacc gttccggcga gaatgtcacc cacggtgacc
     2461 ctcagctcaa gttcttcaac ggcgtagacg ggataggtga actggaacgt gagaaagatc
     2521 agtaccagcg gagcgagaaa gactctcaga aggctcagca tgttcgggat gttccaggtt
     2581 atcttcctca cggatacagc ctcctcgcaa tgatattacg ccttcaagtc tagtatagga
     2641 accggaaaag ggcaacaggg tggaggtcct tcaaaggccc atctttcccg acagagggag
     2701 tgccgccgga ctcgtcctgc cggcgacact cccctggctt tcaccatccg cgggccgttt
     2761 ttcccggtcc ggcgttcatt ttcgggtatc agcctccgag ataggcctcc ttcaccctg
//
LOCUS       DAOTHD010000189         2817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_42290_length_2817_cov_2.722303, whole genome shotgun sequence.
ACCESSION   DAOTHD010000189 DAOTHD010000000
VERSION     DAOTHD010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42290_length_2817_cov_2.722303"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..551
                     /locus_tag="P5219_04305"
     CDS             105..551
                     /locus_tag="P5219_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26010.1"
                     /translation="MKAVRHVLRNFSFRQAVPFAVAGIVVLLALFYMFPVVPDRDEDE
                     FRAMEIIKGLRAVRAAAHMFFEKNPDVPAGPLHIDSLKPYTNADISLFLIERNAQGDW
                     YCSFGHEISENVRKKLKARASEYELLKNASDQYDGGPVVLMAVRKF"
     gene            694..1272
                     /locus_tag="P5219_04310"
     CDS             694..1272
                     /locus_tag="P5219_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HRX26011.1"
                     /translation="MIIYRNAVPDDLAGVAELQKRYHISTIREEDKADGFVTTLFTPE
                     QFLSLMKDENGLAVACDGNKIISYAMAASWEYWSAWPLFRHMIADLPNIEYLGRVLST
                     ENSYQYGPVCIAGEYRGRGVLEHIFEFSRRQMVSRYPVMVTFINRINPRSYDAHTRKV
                     GMDLIKTFDFNGNHYYELGYDMTKPVPGTNLE"
     gene            1479..1694
                     /locus_tag="P5219_04315"
     CDS             1479..1694
                     /locus_tag="P5219_04315"
                     /inference="COORDINATES: protein motif:HMM:NF039402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HRX26012.1"
                     /translation="MGMITLRVSQEEEAVMKGYAESRGMSLSQLMRESILGLIEDEYD
                     IQAYSEYLAYKDNCKMLTLDEAKELWK"
     gene            1685..1957
                     /locus_tag="P5219_04320"
     CDS             1685..1957
                     /locus_tag="P5219_04320"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRX26013.1"
                     /translation="MEVTYRFLVPEPVARKIAAYDKSVRKMLEKYIQKHLVDAEDPRV
                     FGKALKYQHIGLWRYRVGDYRLLVEINDHQLVIIAVDFAHRSEIYK"
     gene            2040..2600
                     /locus_tag="P5219_04325"
     CDS             2040..2600
                     /locus_tag="P5219_04325"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRX26014.1"
                     /translation="MIEAVRTRRSVRKFTGRNVEDELVLQLLESARVAPSGHNTQPWR
                     FIVVRSPEAKRALAKAAHGQSWMEAAPVFIVCVADVRCRIQDEPGLEVKETSPHFALK
                     QVIRDTAIAVEHAVLEAESMGLGTCWVAWYEQADVRPILGIPADKFVVAILPLGYPAE
                     TPGERPRKKLDEIVMFEKWEEKAGKA"
BASE COUNT          686 a          659 c          815 g          657 t
ORIGIN      
        1 gctggggaac ctgctgaagg gacaacagga gcaaaatttc cggtttccaa aggattgtca
       61 gtttccgaaa tgcccaattc caaactccgg aggggaacgc tctcatgaaa gccgtgcgtc
      121 atgttctgcg gaacttttct ttcaggcagg cggtaccttt tgccgttgca ggtattgtcg
      181 tgctgttggc tcttttttac atgttccctg tggttcccga cagagatgaa gacgagttca
      241 gagcgatgga gatcatcaag gggttgaggg cggtcagggc tgccgcgcac atgttttttg
      301 aaaaaaaccc tgacgtaccg gcgggtcctt tgcatatcga ctcgctcaaa ccgtacacca
      361 atgctgacat ctctttgttt ctcattgaga gaaatgctca gggagactgg tattgcagtt
      421 ttgggcatga aatatctgaa aatgtcagaa aaaaactcaa agccagagca agcgagtacg
      481 aattgctaaa aaatgcgtct gatcaatatg acggcgggcc ggtcgtgtta atggcggttc
      541 ggaaatttta ggtatacaaa aacaggaagg gtatttggca cgatggctcg agtaattgtc
      601 cgtccttagc tttccggtct tttgaaccgg ataacgtgtc tcgttttatt ttcttatcaa
      661 aaatcaccga agaattttaa aggaggcttt cccatgatca tctatcggaa cgcggtgccg
      721 gacgatctgg ccggtgtcgc cgaactgcag aagcggtacc acatttcgac catccgggag
      781 gaggacaagg cggacgggtt cgtgaccact ctgttcaccc cggagcagtt cctgtccctc
      841 atgaaggacg aaaacggact ggcggtggcg tgcgacggca acaaaatcat ttcctatgcc
      901 atggcggcgt cctgggaata ctggtccgca tggcccctct tccggcacat gatcgccgat
      961 ctgccgaaca tcgaatacct cgggagggtc ctttccacgg agaactcata ccagtacggg
     1021 ccggtctgca tcgccgggga gtatcgggga cggggagtgc tggagcatat tttcgaattc
     1081 tcccgccggc agatggtgtc ccgctatccc gtgatggtca ccttcatcaa caggatcaac
     1141 ccccggtcct acgacgccca tacccggaag gtgggcatgg acctgatcaa gaccttcgac
     1201 ttcaacggca accactatta cgagctgggg tacgatatga ccaagccggt ccctggaacg
     1261 aacctggagt aattgttttt tcctgctgtt tcaggatact ctttgtatga aataattcgg
     1321 gctgtaggat aaattcttcc gctccttgaa aagaattaca tcgaacagac tgctggccga
     1381 aatgtcggga agagtgcctg ggaagatctt ggttttgctg agtttgacgg aaatacaagc
     1441 gtgttgtaca atgtaataca tggcaaggag gtgttgtcat gggtatgatt actttgcggg
     1501 tttctcagga agaagaggcg gttatgaaag gctacgcaga aagcagaggg atgagtcttt
     1561 ctcaacttat gagggaatcc atactgggcc tgatcgaaga cgaatacgat atacaagcct
     1621 atagtgagta ccttgcatac aaagacaact gtaaaatgct caccttggat gaagcaaagg
     1681 aactatggaa gtgacttatc gttttttagt ccctgaaccg gtggctagga agattgctgc
     1741 atacgacaaa tcggtccgaa aaatgctgga aaaatacata cagaagcatc ttgttgatgc
     1801 tgaggacccc agagtattcg ggaaagcgct gaaatatcag catattggat tatggcgtta
     1861 ccgtgtgggg gattaccgcc ttcttgtgga aattaacgat catcaactgg taataattgc
     1921 cgttgatttt gctcatcgct cagagatcta taaatagttg tgcacaaagg ctgattgaca
     1981 tgtttttctg tgtcagttga gtgaatcgga atatggaaaa tttccgggga ggaatgtaaa
     2041 tgatcgaagc cgtcaggacg aggcgcagcg tgcgtaaatt taccggaagg aacgtggagg
     2101 acgagcttgt ccttcagctt ctggagagcg cccgggtggc gccgtcgggg cacaataccc
     2161 agccgtggcg gtttatcgtg gtgcggtctc ccgaggcgaa acgggcgctg gcgaaggctg
     2221 cccacggcca gtcgtggatg gaggcggccc ccgtgtttat tgtctgtgtg gccgatgtcc
     2281 ggtgccgcat acaggacgag cccggcctcg aggtgaagga gacgagcccc cacttcgccc
     2341 tgaagcaggt gatccgggac acggccatcg ccgtggagca cgcggttctt gaggcggaga
     2401 gcatgggcct gggaacgtgc tgggtggcgt ggtacgaaca ggcggacgtc cgtcccattc
     2461 tcggcattcc ggccgacaaa tttgtggtgg ccatcctgcc cctgggctac ccggccgaaa
     2521 ctcccgggga gcgcccgcgg aagaaactgg acgagatcgt catgttcgag aaatgggagg
     2581 aaaaggctgg aaaagcataa aagacaggat cctcgctccc ctcgggatga tactggtttg
     2641 ttgtcatcct gaggagccgc agacgacgat cgcgcagcat ccccgcaggg ggaggatctc
     2701 gtttttgagg aaggctaaaa ccaaaggcga gatccttcgc tttcgctcag gatgacaagc
     2761 aagggagaga tccctcgcat tcgctcggat gacaccggtc tgttgtcatc ctgagga
//
LOCUS       DAOTHD010000190         2810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_42423_length_2810_cov_2.589474, whole genome shotgun sequence.
ACCESSION   DAOTHD010000190 DAOTHD010000000
VERSION     DAOTHD010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42423_length_2810_cov_2.589474"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1428)
                     /locus_tag="P5219_04330"
     CDS             complement(<1..1428)
                     /locus_tag="P5219_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRX26015.1"
                     /translation="MNLPELSVNRRVSMLMVFLAVILVGGIVFFGLKLDLLPNIEPPV
                     VNVLVTWPGASASDVEQRVTKVLEDKVSLIEGVDTIFSKSMDNISVVSVKFKWGVDLD
                     VKMGDIRDSVNFARRDLPGDAEEPILLRITSGTIPFLTLSFTAERSWEGLHHFVEKTV
                     VENLSRIPGVGQVLVYGGEQREIQVRIDAEKVEAFGIPIGSVIAAVERENLNIPAGSL
                     KQGRTEYYVRVPGRFTSVEEIGRTVVGVSGGRPVHLEDVAEVDDTYKDRDLRGYHFDR
                     EAVIMAVLKTSDANTVEVSRSVLGRLQELKEKEFPSDVDYHIGMNTSEFILNSINNLT
                     RSLLAGIILVFAVTWIFLKRLSASLIVCGAIPFSLVITFIFMGELDYTINIFTLSALA
                     MASGMVVDNCIVSTDQVVYHIEKGARRSVACVIGTAEVQSALVSSTLTTVVVLLPLAF
                     IRGLVGVFFSSLTVVMVAAVAASLFV"
     gene            complement(1425..2513)
                     /locus_tag="P5219_04335"
     CDS             complement(1425..2513)
                     /locus_tag="P5219_04335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRX26016.1"
                     /translation="MKKILALLLFIFLFGGLIIYRWHEKENAGSGSPTREILPVKTVF
                     LKNVIFEDKVSFAAGIEPEEKAAVVCKVPGKTVLRVLVSEGDFVKKGDPLAIVDDSLL
                     RQQILQAEAALGRASSFSSTVAADFGRISALYKEQVVSRQQFDRSRGESRMAARQVQE
                     ARAALNQLKIVLGYHTITAPISGVVLTRYIDPGDTVSQSPAFILARREKVKVSGSVPE
                     HLFPRLKEGQKALVTVDAFPGKQFAAAVSRVHPSLDPVTRTGKVDVLLSAEDSLLPGM
                     YARVTILTGVREGYGLPLEAVGRMAGTGESVCYVVSDGMARLRVIETGAEKDNFLEVL
                     SGLEKREAVIASRSEKLRDGTPVKAAEK"
     gene            complement(2510..>2810)
                     /locus_tag="P5219_04340"
     CDS             complement(2510..>2810)
                     /locus_tag="P5219_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRX26017.1"
                     /translation="LKRQIELEISVARTNFESSLRRIEVGKALVAAAEEDYRMALKRY
                     VAQVGTNIDVLDAAVALANARNQLVEAVYDSRKARAEIDWAMGRTGKAFFTEAAE"
BASE COUNT          600 a          820 c          814 g          576 t
ORIGIN      
        1 cacgaagagg gaagccgcaa ccgccgccac catgaccaca gtgagggatg aaaagaagac
       61 gcccacgagt ccccggatga aggcaagagg aaggagcacc accaccgtgg tgagggtgga
      121 ggacacgaga gcggactgga cttccgccgt gccgatgaca caggcgacgg atcttcgggc
      181 ccctttttca atatgataga ccacctggtc ggtggagacg atgcagttgt ccactaccat
      241 gccgctggcc atggccaggg ctgagagggt gaagatgttg atggtgtagt ccagctctcc
      301 catgaatatg aaggtaatga ccagggagaa ggggatggcg ccgcagacga tgagggaagc
      361 ggaaaggcgc ttgagaaaga tccatgtaac ggcgaagacc aggattatcc ccgcgaggag
      421 ggaccgagtc aggttattga tggaattaag gatgaattcg gaggtgttca ttccgatgtg
      481 gtagtcaacg tccgagggga attctttctc tttgagctcc tgcagccggc cgagcaccga
      541 acgggagacc tcaactgtat tggcgtcgct ggttttgagg acggccatga tgaccgcctc
      601 ccggtcgaaa tgatagcccc tgagatcccg gtccttgtat gtatcgtcca cctccgccac
      661 gtcttcgagg tggaccgggc gcccgccgga aactccgact acggtccgcc cgatttcctc
      721 cacagaggtg aaccggccgg gaacgcggac ataatattcc gtccggccct gcttcagcga
      781 tccagcgggt atattgaggt tttcacgctc cacggcggcg atgacagacc caattggaat
      841 gccgaaggcc tccacttttt ccgcgtcgat ccgcacctgg atttcccgct gctctcctcc
      901 gtagaccagg acctgcccca cgccgggaat cctggagagg ttctccacca ccgtcttttc
      961 gacaaaatga tggagacctt cccaggaccg ttccgctgtg aaggaaagtg taaggaaggg
     1021 gatggtgccg gaggttatgc ggaggagtat cggttcctcc gcatcgccgg ggaggtctct
     1081 ccgggcgaag ttgacggaat cccggatgtc ccccatcttc acatccaggt caacgcccca
     1141 tttgaacttg acggacacta cggagatatt gtccatggac ttggaaaaaa tagtatcaac
     1201 tccctcgatg agagagacct tgtcctccaa gactttggtg acccgctgct ccacgtcgct
     1261 ggcggaagcc ccgggccagg tgacaagcac gttcactacg ggcggctcga tattcggcag
     1321 aaggtcgagc ttgagtccga agaagactat cccccccacg agaatgaccg caagaaagac
     1381 cataagcatg gagactctgc ggttgacgga aagttccggg agattcattt ttccgccgcc
     1441 ttcacggggg tgccgtcccg gagtttttca gacctggaag cgattactgc ctcccttttc
     1501 tcaaggccgg aaagaacttc caggaaatta tccttttccg cccccgtctc aatgactctc
     1561 agccgagcca tgccgtcgga gaccacgtag cagaccgatt ctcccgtccc cgccattctg
     1621 cccactgcct caagggggag gccgtacccc tcccgcactc cggtcaggat cgtcacccgg
     1681 gcatacatgc ccggaaggag ggagtcttcc gcggaaagga ggacgtccac ttttcctgtg
     1741 cgggtgaccg ggtcgagaga gggatgaacc cgggaaacag ctgccgcaaa ctgcttcccg
     1801 gggaaggcgt ccacagtgac cagcgccttc tgcccttcct tcaggcgagg aaagaggtgt
     1861 tcgggcacac tccccgagac tttcactttt tcccgtcggg cgagtatgaa ggccggcgac
     1921 tgggaaacgg tgtcacccgg gtcaatgtac ctggtgagga ccacgccgga aatgggggcg
     1981 gtgatggtgt ggtaccccaa cacaattttc agctggttca aagctgccct ggcctcctgg
     2041 acctgccggg ctgccatccg gctctctccc cgggatctgt cgaactgctg acggctgacc
     2101 acctgctcct tgtaaagggc agatatgcgc ccgaagtcgg cggcgacggt ggatgaaaag
     2161 ctcgaggcac ggccgagtgc cgcttcggcc tggagtatct gctggcggag caggctgtcg
     2221 tccacaatgg caagaggatc ccctttcttg acgaagtcac cttcggaaac caggacacga
     2281 agaaccgttt tgcccggtac cttgcagacc acggcggctt tctcttcagg ttcgatgcct
     2341 gccgcaaaag acactttgtc ttcgaagatc acgtttttga gaaaaaccgt tttcaccggg
     2401 agaatctccc gggtgggcga gccgcttccc gcgttttcct tttcgtgcca tcggtagatg
     2461 atgagcccac cgaaaaggaa gataaacagt agaagagcga gtattttttt cattccgccg
     2521 cctccgtgaa aaaagctttt cctgttcttc ccatagccca gtctatttca gcccttgctt
     2581 ttcgggagtc gtacaccgcc tccacaagct ggttcctggc attcgccagg gctacggcag
     2641 cgtcaagcac gtcgatgttg gtcccaacct gggccacgta ccgtttcagg gccattcggt
     2701 aatcctcttc ggcagcagcc accagggcct ttcccacctc gatcctcctg agggatgact
     2761 cgaagttcgt acgggctacg gagatttcga gctcgatctg ccgcttcaga
//
LOCUS       DAOTHD010000191         2799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_42655_length_2799_cov_2.637391, whole genome shotgun sequence.
ACCESSION   DAOTHD010000191 DAOTHD010000000
VERSION     DAOTHD010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42655_length_2799_cov_2.637391"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..1083
                     /locus_tag="P5219_04345"
     CDS             190..1083
                     /locus_tag="P5219_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26018.1"
                     /translation="MALRADDRPGSGKSFPDGCHYRIEMSGVEGPKVLEALIRERRKR
                     NVPIHRLVSFCQGGTLYDDAELKDFAQMAAEEKMEVIAIPGPRNAWDIGRQFVTPDGQ
                     RCGGLNHRGSDEIRKVIADMLRMYDIGFRGFMLVDRGVLALVGKMQEQGNFPKDIVIK
                     LSVWAGVSSPAGALLAQQLGASSFNPVADLTLPQMAAIRSVTDIPIDFYIWTFDSYGG
                     SNRMYDAPEVAKVYAPCYFKFEPAPTAGYYNPFNSDEEHMRLMEKKVKWAEWVIEHVA
                     LNEPGVRVSPQGAPDLHIPKI"
     gene            complement(1167..2411)
                     /locus_tag="P5219_04350"
     CDS             complement(1167..2411)
                     /locus_tag="P5219_04350"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HRX26019.1"
                     /translation="MKYLGRIACIALLLSLAAGSAAFGAGFALYDFSARGNALGGAMV
                     GRADDPSALALNPAGITQIPGSALMTSAGFLLPYGTVSVAGGGISTDQNDRTFILPNL
                     YYTRQMSDSLWFGIGVMSRFGLGTDFPGDWFGRYNSYRAMIESVSINPNLAWKVNDSL
                     SLSFGVEALWFEADLRKKIDPTGGAASHPATDIDFRLKGDDIAFGWNVGLHYRMDEAT
                     RIGLHYRSRVKLALSGTAAAQWLMGSDSTGGYVDLTLPEMYMFGISRQLTPKLNVEAG
                     AIYTGWDSYESLAFTFDKPLLGILPTRSFSPKNWGSVWRYQIGFEYKHSPEWTWRLGY
                     TYDQEPMPLSTLDYQVPTGDRQLLSVGFGYSKDNWSLDLAYTYLIAGDRYFEARPREG
                     IRNSRSHDMGANIFMISFSMRL"
     gene            complement(2499..>2799)
                     /locus_tag="P5219_04355"
     CDS             complement(2499..>2799)
                     /locus_tag="P5219_04355"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRX26020.1"
                     /translation="NLFPMITVDPEGKACGFGRPGSERSNRKKMLDEFKRLTDRHGLW
                     KYSVLHADAPGAAEELAADLEEACGRAPEYTMEISPVVGINTGPGSAGFALILED"
BASE COUNT          546 a          866 c          852 g          535 t
ORIGIN      
        1 cgcggagagc gaggtgtatg aataggcccc gcttccgttt acaatcgccg ggatgagatt
       61 tttggctttg gcgttggccg cgtgttgcct gctcaccgcc caagacccgc gcaacatccg
      121 caatggttgg gagattccca acgagggcta cgccgaccag ccctacatcg tcaagacgga
      181 tgacggcgct tggctctgcg tgctgacgac cggcccggga gcggaaagag cttccccgac
      241 ggatgccatt accgcatcga aatgtcgggc gtggaggggc cgaaggtcct tgaggccctt
      301 atccgtgagc ggagaaagcg gaacgtgccc attcaccggc tggtctcttt ctgccaggga
      361 ggaaccctct acgacgacgc cgaactgaag gacttcgccc agatggccgc agaggaaaaa
      421 atggaggtca tcgccatccc cgggccgagg aacgcgtggg acatcgggcg gcagttcgtc
      481 acccccgacg gccagcgctg cggggggctg aaccatcgcg gatcggacga aatccgcaag
      541 gttatcgccg acatgctgag gatgtacgac atcggtttcc ggggtttcat gctcgttgac
      601 cggggcgttc tggccctcgt ggggaaaatg caggagcagg ggaacttccc gaaggatatc
      661 gtgatcaagc tctccgtctg ggcaggagtt tcctcccccg ccggggctct tctggcccag
      721 cagctcggcg caagctcctt caaccccgtg gcggacctca ccctgcccca gatggccgcc
      781 atccggtcgg tgacggacat ccccatcgac ttctatatct ggactttcga ctcctacggc
      841 ggatcgaacc gcatgtacga cgccccggag gtcgcgaagg tctacgcgcc atgctacttc
      901 aagttcgaac cggcgccgac cgcggggtat tacaacccct tcaactccga cgaggagcac
      961 atgcgcctca tggagaaaaa ggtaaagtgg gcagaatggg tgatcgaaca cgtcgccctc
     1021 aacgagccgg gggtgcgggt ttcgccccag ggagccccgg atctgcacat tccgaagatc
     1081 tgacggacac atttcctgcc gggaaaaaag cggacccttt tcagggtccg cttgtgtttt
     1141 tccgcccccg tgttctcagg aaccggctac agcctcatgc tgaagctgat cataaaaatg
     1201 ttggcgccca tgtcatggga gcgggaattc cggattcctt cccggggccg cgcttcgaaa
     1261 tatctgtcgc cggcgatcag gtaggtgtag gccaaatcca gggaccagtt gtccttcgag
     1321 tagccgaagc cgacggacag gagctgacgg tctcccgtgg gaacctgata gtcaagggtc
     1381 gacaggggca tgggttcctg gtcgtaggtg tacccaagcc tccaggtcca ctcgggggaa
     1441 tgtttgtatt cgaagccgat ctggtaccgc cagacgctgc cccagttttt cggagagaag
     1501 gacctggtgg gcagtatgcc cagaagaggc ttgtcgaacg tgaaggccag ggattcatag
     1561 ctgtcccagc cggtgtagat ggccccggcc tccacattga gcttcggggt aagctgcctg
     1621 ctgatgccga acatgtacat ctccgggagg gtgaggtcca cgtacccacc cgtggagtcc
     1681 gaccccatga gccactgggc ggccgctgtc cccgaaaggg ccagcttcac cctgctccgg
     1741 tagtggagac ctatcctcgt cgcctcgtcc atcctgtagt ggagccccac gttccatccg
     1801 aaggcgatgt cgtctccctt gagccggaag tcgatatccg tcgcaggatg tgaagccgct
     1861 ccccccgtgg gatcgatctt ttttctcagg tcggcctcga accacagggc ctccacgcca
     1921 aaggaaaggg agagggaatc attgactttc caggcgagat tcgggttgat cgataccgat
     1981 tcgatcatcg cccggtagct gttgtacctg ccgaaccagt ccccgggaaa atcggtcccg
     2041 aggccgaagc gggacatgac gccgatgccg aaccagaggg agtcgctcat ctgtctcgta
     2101 tagtacagat tcggcaggat gaaggtcctg tcattctgat cggtggaaat acctccgcct
     2161 gcgacagaga cagtgccgta gggcagcaga aagcctgcgg acgtcataag ggcgctcccc
     2221 gggatctggg tgatgccggc cgggttcagg gcgagggccg acggatcgtc cgcccgcccc
     2281 accatggccc cgccgagggc gttccccctg gcgctgaagt cgtagagggc aaacccggcc
     2341 ccgaatgccg cagaacctgc cgcgagagac aacaaaagcg cgatgcatgc gatacgtccc
     2401 agatacttca aaactttcca ccccttccgg gcatgtcctg cggcaccgac ggcttgaaac
     2461 cggtccagcc gcaccacgcc ccccagattt ttttcgcttc agtcttccag gatcagagcg
     2521 aacccggccg atccgggccc cgtattgata cctaccacag gtgaaatctc cattgtgtat
     2581 tcaggggccc tgccgcaggc ttcttccagg tccgccgcca gttcttccgc cgcaccgggg
     2641 gcgtcggcgt ggaggaccga gtatttccac aagccgtgcc ggtcggtcag ccttttgaac
     2701 tcatcgagca tcttcttcct gttggaccgt tcgctgccgg gccgtccgaa gccgcaggcc
     2761 ttcccttccg ggtccacggt gatcatgggg aacaggttc
//
LOCUS       DAOTHD010000192         2773 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_43143_length_2773_cov_3.986019, whole genome shotgun sequence.
ACCESSION   DAOTHD010000192 DAOTHD010000000
VERSION     DAOTHD010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43143_length_2773_cov_3.986019"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="P5219_04360"
     CDS             complement(<1..616)
                     /locus_tag="P5219_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRX26021.1"
                     /translation="MSRYDFDTVIERRGTICAKWDGMEPIFGSGDLLPMWVADMDFKA
                     PPEVIEVLRERVDHGIFGYTRRFAPYREAIAGWFERRHGWKPDTEWIRHTPGVVPALA
                     AAILAFTQPGDGVLVQPPVYYPFFATITGRGRRVVENPLIFTGSRFEMDFADLEKKLD
                     DSSVKLVFLCSPHNPCGRVWTREELERFARICASRDAAAAPPPAV"
     gene            821..>2773
                     /locus_tag="P5219_04365"
     CDS             821..>2773
                     /locus_tag="P5219_04365"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HRX26022.1"
                     /translation="MVFSYKDRFRSSFPPPPAAGGESGLSENALWLLDQRYFARRYDP
                     ESGTTRKETSFGEFARRVARIVASAETAYLGGSGSDLEWLRRLENSIYDDITHRRFLF
                     NSPCLFGAGAGMTVDPVLSGKIYCPPEEMGFGDYDLLRRSRTRSQQLFACFVIDVPDS
                     IDGIFDGVKDAAVISKYGGGVGGNFGHLRERGSAINGGIGGQASGPVSFMETWNTMGA
                     VVVQGGRRRAALMGMLFDDHPDIYDFIDAKVEEGKLPYFNISVCVSDKLMESAEHGGD
                     FSLVSRSDGKTVRRVKAEDLWNKLCESAWKRGDPGIFFIDRANDDNLLKLGDTWRIES
                     TNPCGEQPLPNYTSCNLGSVNVEAFVSTAADGRKTFDFSAFVDQVFRSMYYLDLVIDA
                     CSYPLERIEERTKRIRPVGLGIMGLADAAILLGMRYGSPRFSEFCKCLGDSMGSAALC
                     AAAGIVSDMGKEPFPEAVLVEELFSKFAGEQGEELFPESWYGRLDRDGYRSLLEKMRS
                     SATIPYTLSNALEGFFTAQGLGDEGARLELAGSVLLAFAKGRIRNSRRLSVAPTGSIS
                     MLIDTSAGIEPNFAWSWTRRIARADGEGQETREFCHKLLSPSQVDELHEKGALSDPVF
                     VTAYDIPPDEHVEVTGIFARVVDSGIS"
BASE COUNT          534 a          791 c          880 g          568 t
ORIGIN      
        1 tcaccgcagg gggcggcgcg gccgccgcgt cgcgggaggc gcagatgcgg gcgaaccttt
       61 ccagttcctc cctggtccag accctcccgc agggattgtg gggcgaacac aggaagacga
      121 gcttcaccga tgagtcatcc agctttttct ccaggtcggc gaagtccatc tcgaaacggc
      181 tgccggtaaa aatgaggggg ttttccacca cccggcggcc acgccctgtg atggtggcga
      241 agaaggggta atagaccggc ggctggacga gcaccccgtc tccgggctgg gtgaaggcga
      301 ggatggctgc cgccagggcg ggaaccacgc cgggggtgtg gcggatccac tctgtgtcgg
      361 gtttccagcc gtgtcttcgc tcgaaccagc ccgcaatggc ctcccggtag ggggcgaaac
      421 ggcgggtata gccgaaaatg ccgtggtcca cccgttcccg gagaacctcg atgacctccg
      481 gcggggcctt gaagtccatg tcggcaaccc acatggggag aagatcgccg gagccgaaga
      541 tgggctccat gccgtcccac tttgcgcaga tggtgccgcg ccgttcaatg acggtgtcaa
      601 aatcgtagcg gctcattttt gtacctctct ttctctcatt atgcatattt tggacagtga
      661 gtttttaata tcatatcatt ttcttccaat ttggaagttt ttcctccctc ttgcctcacc
      721 tcccgggaag tggtagtctg atgtcctgtt cataaccaca cacaagatat tgtgtgtgaa
      781 atcattccca tgctcctaat agtgtggagg aggatacttc atggtttttt cgtacaagga
      841 caggttccgt tcttcttttc cgccgccccc cgcagcggga ggagaaagcg gtctctccga
      901 gaatgcgctc tggctgctgg accagcggta ttttgcccga cggtatgatc cggaatcagg
      961 cacaaccagg aaagaaacat ccttcggaga atttgcccgg agggtagcca ggatcgtggc
     1021 aagcgcagag acggcctatc tcggcggaag cgggtctgat ctcgaatggc tccgccgtct
     1081 tgaaaacagc atctacgatg acatcacgca caggaggttc cttttcaact cgccctgcct
     1141 tttcggagca ggggcgggaa tgactgtgga tcccgtgctt tcgggaaaga tttactgtcc
     1201 ccctgaagag atgggcttcg gggactacga ccttctccgc cgctccagga cgcggagcca
     1261 gcagctcttc gcctgtttcg tcatcgatgt tcccgacagc attgacggta tcttcgacgg
     1321 agtgaaggat gccgccgtga tcagcaagta cggcggaggc gtggggggca atttcggcca
     1381 tctccgggag cggggcagcg ccatcaacgg cggcatcggc ggacaggcca gcggccccgt
     1441 ttccttcatg gagacctgga acaccatggg tgccgtggtc gtccagggag gacggcggcg
     1501 ggcagccctc atgggcatgc tcttcgacga tcatcccgat atttacgact tcatcgacgc
     1561 caaggtggag gagggcaagc ttccctattt caacatctcc gtgtgcgttt cagacaagct
     1621 catggagtcg gcggagcatg gaggggactt cagcctggtg tcccggagcg acgggaagac
     1681 ggtcaggcgg gtgaaggcgg aggatctctg gaacaagctc tgcgagagcg cctggaagcg
     1741 gggggacccc ggcatcttct tcattgacag ggccaacgac gacaacctgc tgaagctcgg
     1801 ggacacatgg aggatcgaat ccaccaatcc ctgcggcgaa cagccccttc cgaactacac
     1861 aagctgcaat ctcggctcgg tgaatgtgga ggcctttgtc tcgacggccg ccgacggaag
     1921 gaaaaccttt gatttcagtg ccttcgtgga ccaggtcttc cgctccatgt actatctcga
     1981 cctggtgatc gatgcctgct cctaccccct ggagaggatc gaggagagaa cgaagaggat
     2041 acgccccgtg ggcctcggga tcatggggct ggcggacgcg gccattctgc ttggaatgcg
     2101 gtacggttcc ccgaggttca gcgaattctg caaatgcctg ggcgactcca tggggagcgc
     2161 ggccctctgc gccgccgccg gaatcgtgtc ggatatgggc aaggagccct tcccggaggc
     2221 ggttcttgtg gaggagcttt tttcgaaatt tgccggggag cagggagagg agcttttccc
     2281 cgaatcgtgg tacggcaggc tcgaccggga tggataccgt tccctgctgg agaagatgcg
     2341 gtcttcggcc acaatcccct ataccctgtc gaacgctctc gaaggctttt tcaccgccca
     2401 ggggctcggg gacgaaggag cgcggctgga actggcgggc agcgttctgc ttgccttcgc
     2461 gaagggaagg atccggaaca gccggaggct ctccgtcgca cccacgggct ccatttccat
     2521 gcttatcgac accagcgccg gcatcgagcc gaacttcgcc tggagctgga cccggcggat
     2581 agcccgggct gacggtgagg gacaggagac gagggagttc tgccataagc ttctctctcc
     2641 gtcccaggtt gacgagctgc atgaaaaggg tgccctttcc gacccggtct ttgtgacggc
     2701 ttatgacatt cctccggatg agcacgtgga ggtgacgggc attttcgccc gggtggtgga
     2761 cagcggcatc agc
//
LOCUS       DAOTHD010000193         2773 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_43159_length_2773_cov_2.515085, whole genome shotgun sequence.
ACCESSION   DAOTHD010000193 DAOTHD010000000
VERSION     DAOTHD010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43159_length_2773_cov_2.515085"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="P5219_04370"
     CDS             <1..772
                     /locus_tag="P5219_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRX26023.1"
                     /translation="GAQAAAKAASEAGEGAAQIAEAARKGGESVQEMADMITQTAKAG
                     EQVGNAVENLAGTVKNISGFVATITQIADQTNLLALNAAIEAARAGEAGRGFAVVAEE
                     VRKLAEESNRSAGEVGKLISEVTSRTESALKDSEGSSRILKELVAKASETRGAIDNVV
                     KSMNNVTENIQTIAATVEEQSASAQEMTAGMDSVSKSSIEIAAQIASVNSSMQEQTKA
                     VEAIAASSEQLVSLSNAMQQAVSRFKVSEEDRGLVVRK"
     gene            1208..2242
                     /locus_tag="P5219_04375"
     CDS             1208..2242
                     /locus_tag="P5219_04375"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HRX26024.1"
                     /translation="MHKRRERIITSKRSVFILTAVLTLFLIPLFSGPSCAAPGYSFDP
                     NLAPPPPDYSSPKSWAVLPKAPLPFAVDVFFVHPTTYNGDENWNQSLEGEAADRKVQG
                     IMSGQAGVFSTSACLYAPYYRQANLAVLHTPEDSPERKSLDTAYSDVEAAFDIYMSTW
                     NRGRPFILAGHSQGSQHLLRLMERRLGDPETGKLLVAAYIIGWSVTRDDLARHPHLKM
                     AETPDQTGCIVSYNTQGPKPGYSIVREGAVAVNPLTMTITGEPADAGLNLGAVFCDEK
                     GVREIPHYTGGQTINGAFVIPAPSNVKDLDAIQIPEFYHPYDYAFFFRNLQKNAELRT
                     KKILDSEAGH"
     gene            2325..2669
                     /locus_tag="P5219_04380"
     CDS             2325..2669
                     /locus_tag="P5219_04380"
                     /inference="COORDINATES: protein motif:HMM:NF024076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HRX26025.1"
                     /translation="MMNEQALRMTDADHPPTPEELGEWLGSDAYSFWERFSRFIGETY
                     PGVFSPEWLFGGKKHGWSLRYKKSRSFCTMVPERERFSLVIVFGAEERAKAEAILPRL
                     SEETGIKLPSPQ"
BASE COUNT          650 a          805 c          822 g          496 t
ORIGIN      
        1 gggagcgcag gcagccgcaa aagccgcctc ggaagccggg gaaggggccg cgcagatcgc
       61 tgaggcagcc cggaagggcg gagaatccgt ccaggaaatg gccgacatga tcacccagac
      121 ggcgaaagca ggagaacagg tcgggaacgc cgtggagaac ctcgccggaa cggtgaaaaa
      181 tatttccggc tttgttgcca ccatcaccca gatagcggac cagaccaacc tactggcgct
      241 gaatgcggcg atcgaggccg cccgtgccgg agaggcgggc cggggcttcg cggtcgtggc
      301 ggaagaggtc aggaaactcg cggaggagtc caaccggtcc gccggagagg tcgggaaact
      361 gatctccgag gtcacgtccc gcaccgagag tgcgctgaaa gactcggaag gttcgtccag
      421 gatcctgaag gagctggtcg cgaaggcctc ggagacacgg ggcgcgatcg acaatgtcgt
      481 gaagagcatg aacaatgtga cggagaacat ccagaccata gctgccaccg tcgaagagca
      541 gtcggcaagc gcacaggaaa tgaccgcagg tatggacagc gtatcgaagt cgagcattga
      601 gatagcagcg cagatagcca gcgtgaacag ttcgatgcag gagcagacaa aggcggtgga
      661 agccatagcc gcttcctccg agcagcttgt gagcctgagc aatgccatgc agcaggccgt
      721 gtcccgcttc aaggtgagcg aagaggaccg ggggcttgtg gtgaggaagt aggcggacgc
      781 ctctcctttc ctgatttccc ggcgtgagcc cggggcaaac tcttcagccc gatggcggaa
      841 aagtcatcag ggaaacaggc agctatacaa acacggcgtt tccgggacac ccccggaaac
      901 gccgtgtttc attcctcaac ctttcggccg aaaacgcctc tccgccggaa gttcacgttt
      961 ctctttgtgc atatttgtac tattattgag gaaaataaac ggaaccttcc ggaacgccag
     1021 ccccatccgg aaagaacacc ggcaaaccac aggaaaaggg gaaggaagtg gtgcggcgtg
     1081 aggcaatttt ctgtcttggg atgcccctgt ttttttggcc ggttccttgt ccgccgctga
     1141 tttcggaagg cgggacgagg caaatccgga aacagcgcac ttcaggtcct gcaacggaat
     1201 tttgaccatg cacaaacgaa gggagagaat cattacgtcc aaacgatctg tttttatcct
     1261 cactgcggtg ctgacactat tcctgatccc ccttttttcc gggccttcct gcgccgcacc
     1321 ggggtattct ttcgacccga acctggcgcc ccctcccccc gactactcgt caccaaagag
     1381 ctgggcggta ctgccgaagg cacccctccc tttcgccgtg gatgttttct tcgtccaccc
     1441 cacgacttat aacggcgacg agaactggaa ccagtcactt gaaggagaag ccgccgaccg
     1501 caaggtccag ggcatcatgt cgggacaggc cggagtcttt tctacctccg catgcctgta
     1561 cgccccgtac taccgccagg cgaacctggc cgtcctgcac accccggaag acagccccga
     1621 aaggaagtcc ctcgacacgg cctattctga cgtggaagcc gccttcgaca tctacatgtc
     1681 cacctggaac aggggcaggc ccttcatcct ggccggacac agccagggct cgcagcacct
     1741 actgcgcctg atggaacgcc gtttaggcga ccccgaaaca ggaaaactgc ttgtcgcggc
     1801 ctatatcatc ggctggtccg taacccgtga cgatctcgcc cggcatcctc acctgaaaat
     1861 ggcggagacc ccggaccaga cgggctgcat cgtcagctac aacacccagg ggcccaaacc
     1921 ggggtacagc atcgtcagag aaggcgctgt cgccgtcaac cccctcacca tgaccatcac
     1981 gggagaaccc gctgatgccg ggctgaacct gggggccgtg ttctgcgacg agaagggggt
     2041 ccgggaaatc ccgcattata cgggagggca gaccatcaac ggggccttcg tgattccggc
     2101 gccttccaac gtgaaagacc tggacgcaat ccagattccg gagttctacc acccctacga
     2161 ctacgccttc tttttccgga atctccagaa gaacgccgaa cttcggacaa agaagatcct
     2221 ggattccgaa gccggacact gagatccgcc aatcctccga agacggcgaa agaaaagagg
     2281 ggatgacgca gaccggcgtt ggctcggaag agaaggaggg gaaaatgatg aacgaacagg
     2341 ctctccgcat gaccgatgcg gatcatcccc cgactcccga ggaactcggg gaatggctcg
     2401 ggagcgatgc gtattctttc tgggaacggt tttcccgctt tatcggggag acctatcccg
     2461 gcgtgttctc tcccgaatgg ctcttcggcg ggaagaagca cggctggtcc ctgaggtaca
     2521 aaaagagcag gtccttctgc accatggtcc cggaaaggga gcgtttctct cttgtgatcg
     2581 tcttcggcgc ggaggaacgg gcgaaggctg aagcgatcct gccccggctg tcggaagaga
     2641 cgggaatcaa gctgccctct ccgcagtaag catttgcggc ataagaaaag aacgtacaga
     2701 gtcgtacgtt cttttcttat tcttttgttg atgaagtaac aagagagaaa atgtatgctg
     2761 aagtaatcgc ttt
//
LOCUS       DAOTHD010000194         2768 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_43262_length_2768_cov_3.698489, whole genome shotgun sequence.
ACCESSION   DAOTHD010000194 DAOTHD010000000
VERSION     DAOTHD010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43262_length_2768_cov_3.698489"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="P5219_04385"
     CDS             complement(<1..291)
                     /locus_tag="P5219_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26026.1"
                     /translation="MKLNRLATRILAVVIVLLALAVGGIGFYAMHIGGRGLEDSAGQY
                     EITLAESLSYRLDATFNKFDGMLQALSALVTTRFTSLPNPEVADIVVPQFGAQ"
     gene            complement(462..893)
                     /locus_tag="P5219_04390"
     CDS             complement(462..893)
                     /locus_tag="P5219_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HRX26027.1"
                     /translation="MLNLSEVLKAGLSAEVKKTVEIEDTIGNSSPYLNQYLSTSACAA
                     LAVQAAMAVTENLLPEGYLSVGRRIDLEHEIPAMLGTTVTVKAVLREIRGNRLIFDVT
                     GTDALGTIFRGVNERVVVNRLGLDEKGAERAQHLKELKERL"
     gene            complement(933..2414)
                     /locus_tag="P5219_04395"
     CDS             complement(933..2414)
                     /locus_tag="P5219_04395"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRX26028.1"
                     /translation="MDRTDAAPEKSAFEAGELVSCLMAGMAEFVFIHTLAGKIREVNP
                     AAVKGLGFTAQELLSSDMTAVLASPPPLRLKQAWKNLDTGHPFSSEGIIRRKDGSSFQ
                     AMLTFFPLVRGEEQAVLTIARDVPELKAAGEILSRNAFKDPLTGLYNRAFFEEELKRL
                     DCDRQLPLSVIMGDLNGLKMANDAFGHQVGDNLLKAAAKVLRKICRSSDLLFRWGGDE
                     FVILLPHTREEDAASIVGRIEDAFKKIQVKDMPVPPSMSLGYSAKLHRWQDFANVFRD
                     AEEEMYDKKTAESRKIRETILENIFASLAEDTPETAEHNLSVRRLCRMLGKQIGLDRL
                     DLEKLDLAALLHDIGKASVPSKLLVKTAPLTDEEREDVKRHAEAGYKVASAATPDVAS
                     VADEILSHHERWDGTGYPSGLRGEDIPLLSRIIALADAFDVMTRGTPYRPAKTRDKAL
                     REVKEQAGRQFDPNLAALLLEIAARLDEEPEDSLPPVRDFTIE"
     gene            2572..>2768
                     /locus_tag="P5219_04400"
     CDS             2572..>2768
                     /locus_tag="P5219_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRX26029.1"
                     /translation="MRIVGFCNLKGGVGKTTACQNLAVALARRGLRVAALDLDPQSNL
                     TAGFGIAVPEEHPYIYDFLLG"
BASE COUNT          501 a          831 c          821 g          615 t
ORIGIN      
        1 ctgggcgccg aactggggca ccacgatgtc cgccacttcg ggattgggaa ggctggtgaa
       61 cctggtggtg acgagggccg acagcgcctg gagcatgccg tcgaacttgt tgaaggtggc
      121 gtccagccgg taggaaaggg attccgccag ggtgatctcg tactgcccgg ccgaatcctc
      181 gagcccccga ccgccgatgt gcatggcgta aaacccgatc ccccccacag cgagcgcaag
      241 aagcacgatg accacagcca gaatgcgcgt tgcgagtctg ttcagtttca aggaagaact
      301 ctcccctctg aagatgattg tggaaaaaag cctccatcac tgaaggctcc gtgcacacac
      361 caaacctgca acagtccgga tgtcccggcc tgaaaaccgg agatcagggg gtgtgcgttc
      421 cgggcctgcg cccggtgatt cctttggagt gcagaagaat gctacagtcg ttccttgagt
      481 tccttgaggt gctgcgcccg ctccgcacct ttctcgtcca ggccgaggcg gtttaccaca
      541 acccgttcat tcacgccgcg gaaaatggtc ccgagggcgt cggtgcccgt gacgtcgaag
      601 atgagcctgt tcccccggat ttcccggaga acggccttca cggttaccgt ggtgccgagc
      661 atggccggga tctcgtgttc caggtcgatc ctccggccta cggaaaggta tccctccgga
      721 agcagatttt ccgtcaccgc catggctgcc tggacggcca gcgccgcaca ggcagaagtg
      781 gaaaggtact ggttcaggta gggagatgaa ttgccgatgg tatcctcgat ttccaccgtc
      841 ttcttcacct cggcgctcag ccccgctttc aggacctcgg aaaggttcag catggagcat
      901 ccctccttca gttgttatac tctctccaca gtctattcta tcgtaaaatc cctcaccggg
      961 ggcaaggaat cctccggttc ttcatccagc cttgccgcga tttccaggag cagggcggca
     1021 aggttcgggt caaactgccg tcccgcctgc tccttcactt ccctgagagc cttgtcccgg
     1081 gttttggccg gccggtaggg agttccccgg gtcatgacgt cgaatgcgtc ggccagggcg
     1141 atgatcctgg agaggagggg aatgtcctcc ccccggaggc cggaggggta tcccgtaccg
     1201 tcccaccgtt cgtggtggga aaggatttca tccgccacgg aggccacgtc cggagtggcg
     1261 gcggaggcca ccttgtatcc cgcctccgcg tgccgtttca cgtcctcccg ttcttcgtcc
     1321 gtcagggggg ctgttttcac taaaagtttc gacgggaccg aagcctttcc gatatcgtga
     1381 aggagtgcgg cgagatcgag tttttccagg tccagcctgt ccaggccgat ttgtttgccc
     1441 agcatgcggc agagccgtct caccgaaagg ttgtgttccg ccgtttcagg cgtgtcctcc
     1501 gcaagggacg cgaaaatgtt ctcgaggatg gtctccctga tctttctgct ttccgccgtt
     1561 ttcttgtcgt acatttcctc ctcggcgtcc cggaacacgt tggcgaaatc ctgccagcgg
     1621 tggagcttcg cgctgtatcc gagggacatg ctgggaggga cgggcatgtc cttcacctgg
     1681 atctttttga aggcgtcctc gatgcggccg acgatggagg cggcgtcctc ctcacgggta
     1741 tggggcagca gaatgacgaa ttcatcccct ccccagcgga agaggaggtc gctgctcctg
     1801 cagatttttc tcaggacttt cgccgccgcc ttcaggaggt tgtcccccac ctggtgcccg
     1861 aaagcgtcgt ttgccatttt cagcccgttg agatcaccca tgatgacgct gagaggaagc
     1921 tgccggtcgc agtcgagccg tttcagctcc tcctcgaaga aagccctgtt gtacagcccg
     1981 gtaagagggt ccttgaaggc gttcctggac aggatctccc cggcggcctt gagctccggg
     2041 acatccctgg cgatggtcag gacggcctgt tcctcacccc ggacgagggg gaaaaaggtg
     2101 agcatcgcct gaaacgagct gccgtccttt cggcggatga ttccttccga ggagaaggga
     2161 tggcccgtat ccaggttttt ccaggcctgc ttcagccgga ggggcggcgg ggacgccaga
     2221 actgccgtca tgtctgacga cagcagttcc tgggcggtga aaccgaggcc cttcaccgcc
     2281 gctggattga cctccctgat tttcccggcg agggtatgga taaagacgaa ttcagccatc
     2341 cccgccataa ggcaggacac aagctctccg gcctcgaagg ctgatttttc gggagcggcg
     2401 tctgtgcggt ccattctctg ttcctccttc ttttttgcct gaaaaaatgc attatccgtt
     2461 caattatact ccagggcccg ggaaaaacgg gtctttcccc cttcggtgtt tcttgctata
     2521 ttgtactggg atgccactga acttttcatt cccagatcga ggaggttttt catgagaatc
     2581 gtcggattct gcaatctgaa gggcggcgtg gggaagacca ccgcgtgcca gaatcttgcc
     2641 gtggccctcg cccggagagg actccgggtc gccgctctcg atcttgaccc ccagagcaat
     2701 ctcacggccg ggttcggcat tgccgttccg gaagagcatc cgtatatcta tgatttcctt
     2761 ctcgggga
//
LOCUS       DAOTHD010000195         2751 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_43596_length_2751_cov_4.010015, whole genome shotgun sequence.
ACCESSION   DAOTHD010000195 DAOTHD010000000
VERSION     DAOTHD010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43596_length_2751_cov_4.010015"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            662..1987
                     /locus_tag="P5219_04405"
     CDS             662..1987
                     /locus_tag="P5219_04405"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX26030.1"
                     /translation="MIVRFSIENWMSFRDRAEFSMIATRERQHTERVPRIRKYRTGIL
                     PVTALYGGNASGKSNLFEALRFTRDLIVGGKRPGERIPVKTFRLDEKSREQPAFFSFE
                     LLVDETVYGYSFSLTPKEILEEKLAEITSSGEKTLFHRRGGKAEFHGSLKKNRDLEVY
                     FRGTRENELFLTNSVFQKVNAFLPVWSWFRDNLVLIGPESRFSPFERLGDEQDPLSSS
                     VNDVLARLDVGLHGIEGVEIPLDTVPLSGSAKEKLLEEVREKGNVRLKFSGGERLLLT
                     NENGKIRARKLAARHRGKDGTEVLFDLQDESDGSIRMIDLLPALHALCVPKSAKVFVI
                     DEIDRSLHTLLTRSLLEMFLSSCSAESRSQLLFTTHDALLMDQELLRRDEMWVAERNS
                     EGASRIFSFSEYKDVRYDKDIMKSYLQGRLGGVPRILLCGRESGRENVD"
     gene            1987..2622
                     /locus_tag="P5219_04410"
     CDS             1987..2622
                     /locus_tag="P5219_04410"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HRX26031.1"
                     /translation="MRSGRRLQRPLGERRYKKLFVIAVEGEKTEPLYFSIFNDDSRVV
                     HVRCLEGKTESAPPHVLRRMVRYLADEEADLKKTDEAWLVVDKDQWSEQQLLPLHQWV
                     SKRKRNGPERGFAVSNPSFEYWLLLHFEDGNDIADLRQCIHRLRHHLPESGKSIDPQG
                     FSEEMILKAVERARKRNNPPCTDWPRNPGCTTVYLLVEKILEAGKKNGGRE"
BASE COUNT          673 a          676 c          799 g          603 t
ORIGIN      
        1 agcccggccc atgcaggcct gagtctgcag cagaagaccg acgtctggcg tgcggtggcc
       61 gaggcggctc ggcagggcac ataaacgaaa aggcgcagtt ctgcctccct gagcaaggtt
      121 gccatcctgg gcctgggttg tcatcctgag cgaatgcgaa ggatctcggg cctgagtctc
      181 tcctgaatca aaagcgagat ccttcggcca aaaggctggc ctcaggatga caaaaaagtg
      241 ctgtcatcct gagcgaagcg atcgcgaagc atccccggag gggaaggatc tagcacttga
      301 gtctcttcag aatcaaaagc gagatccttc ggccgagaaa ccggcctcag gatgacaaaa
      361 aagggttgtc attccgggcg aatgcgatcg ctttgctcgc tcgggacgac agggtgtagc
      421 ttgtcatcct gagcgaagcg aaggatctag cacttgattc tcttcagaat caaaagcgag
      481 atccttcggc cgagataccg ccctcaggat gacaaaaaag ggctgtcatc ctgagccggt
      541 tgaattggaa tcaacctctt ttatagagaa ataatctctt ttcatgttgt gatattttca
      601 tttatggttt aatatcatgc aacgaaagag aaattttctc ttaaaaggag gtgcttgtgc
      661 cttgatcgtc aggttttcca tcgaaaactg gatgtccttc cgcgacaggg cggagttctc
      721 catgattgct acccgcgaac ggcagcacac ggagcgtgtt ccgcgaatcc ggaaataccg
      781 gaccggaatt ttgcctgtca ccgcactcta tggcggaaat gcctcgggaa agagcaatct
      841 tttcgaagcc ctgcgtttta cccgtgatct gattgtcgga ggaaaacgcc cgggcgaacg
      901 aattcccgtc aaaacattcc ggctcgacga aaagagcagg gagcagcctg ctttcttcag
      961 ctttgaactg ctcgtggacg agacggtcta cggttattcc ttttccctca cgccgaaaga
     1021 aatcctggaa gaaaaactcg cggaaattac aagttcgggc gaaaagacgc tctttcaccg
     1081 cagaggggga aaagcggaat ttcatggttc cctgaagaaa aacagggacc ttgaggtcta
     1141 tttcaggggt accagggaga atgaactctt tctgacaaat tcggtttttc agaaggtcaa
     1201 tgcctttctt ccggtctggt cctggttcag ggacaacctg gtcctgatcg gacctgaatc
     1261 caggttctcg cctttcgaac gcctgggcga cgaacaggac ccgctttctt cctcagtcaa
     1321 cgatgtgctg gcacggctgg atgtaggcct ccacgggatt gaaggggtgg agattcccct
     1381 tgatacagtg cctctgtccg gttccgcgaa agaaaaactg ctggaggagg tcagggagaa
     1441 ggggaacgtt cggctgaagt tttccggagg cgagcggctt ctgctgacaa acgaaaacgg
     1501 aaagatccgg gcgcggaagc ttgctgcccg gcatagggga aaggatggca ccgaagtcct
     1561 gttcgacctc caggatgagt ccgacgggtc catacgcatg atcgacctgc tcccggcgct
     1621 tcatgcgctc tgtgtcccga aatcggcaaa ggtctttgtc atcgatgaaa ttgaccggag
     1681 cctgcacact cttctgacgc ggagcctgct cgaaatgttt ctttcaagct gttctgcaga
     1741 gagccggtcc cagcttcttt tcaccaccca tgacgccctg ctgatggacc aggagctgct
     1801 gcggcgggat gaaatgtggg tggcagagcg gaattcagag ggagcgtccc gtatcttctc
     1861 attcagtgaa tacaaggatg tccgatacga caaggatatt atgaaaagct acctccaggg
     1921 caggctgggc ggagtgccga gaatcctgct gtgcgggaga gagagcggca gggagaacgt
     1981 tgactgatga ggtcggggag aagattgcag cgccctttgg gagagcggcg atacaaaaaa
     2041 ctctttgtga tcgccgttga aggggaaaag accgaacctc tctatttcag catcttcaac
     2101 gacgacagcc gggtggtgca tgtacgttgc cttgaaggga agacggaaag cgctcctccc
     2161 catgttcttc gaagaatggt ccggtacctg gcggacgaag aagccgattt gaaaaaaacg
     2221 gacgaagcat ggcttgtggt ggataaggat cagtggtctg agcagcaatt actgcctctt
     2281 catcaatggg tatcgaaaag aaaaaggaac gggccggagc gggggtttgc agtcagcaat
     2341 ccgagtttcg aatactggct tctgctccat ttcgaggatg gaaacgatat tgccgatctc
     2401 cggcagtgca ttcacagact ccggcatcat ttgccggagt ctggaaagtc cattgatccg
     2461 caggggtttt cggaggagat gatcctgaaa gccgtggaac gggcccgaaa gcgtaataat
     2521 ccaccctgta cggattggcc ccggaatccc ggatgtacca ctgtttacct gctcgtcgag
     2581 aagattcttg aagccgggaa gaaaaacggg gggagggaat aacccctcac ttgcatttaa
     2641 tgcttccttg agaatggctg tcattccggg cgaatgcgat cgctttgctc gttcgggacg
     2701 acaggaccag gcagtcatcc cgagcgaacg cgagggatct cgaatttgaa c
//
LOCUS       DAOTHD010000196         2749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_43634_length_2749_cov_4.106533, whole genome shotgun sequence.
ACCESSION   DAOTHD010000196 DAOTHD010000000
VERSION     DAOTHD010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43634_length_2749_cov_4.106533"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="P5219_04415"
     CDS             <1..650
                     /locus_tag="P5219_04415"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRX26032.1"
                     /translation="DKPSGVVVHPAPGNRHGTLVHGLLHRFRHFGSFNNVLRPGIVHR
                     LDASTSGLLVVAREQEILENLQNQFRLREVRKQYLALVHGRVKNASGRIDAPIGRCPT
                     NRLRMAVVEDGKPAVTGFRRLWVRNGYSFLECDIATGRTHQIRVHLSSMGHPIVGDVL
                     YGADKKKAASAGRVFLHSWKLSFLHPKTGERLFFTSCLPAALTGQLREILSTSPD"
     gene            complement(583..2271)
                     /locus_tag="P5219_04420"
     CDS             complement(583..2271)
                     /locus_tag="P5219_04420"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HRX26033.1"
                     /translation="MSIGPELFTALARSIGETVLPCRIGKIETGDTWAAFRLSSGKDR
                     WLLFSWNPGSYGCGLVGEDGIASLKKTRDSRSSFGEALKRNFQNASILSVRQLNSDRV
                     LHFAAERLVGAGFSVPLSLVFEGTERNSNLVILDESGSILEPAKHIHGDMNRYRMILP
                     GIAYTPPPPLKGKYLGETGEIEGPEDLRGLRGIGRGLSEILTRFWDTRPVRHWNDMVA
                     RTLSGEEQGLLILRQGSLLTVFPEPLPSCEVLPGPLLERCGEEVLSSYFLGQRERILS
                     GVKKALEREIKSRKRHRDGLENQICLSERGNEFLKMGNLLLAAGDLVPPRARAVELQD
                     WETGEPLLVPLDENLTASRNAEKYFRKYKKGKVDRDAVRKAILSIEEGISELEEQVET
                     LESIDDPDLLSVSARDVMEWLVPKKTTSARKHKTAPPPHIRLENGGDLIYIGLNARGN
                     RHVTFKVAAPTDLWFHVHEIPGAHVILKTAGKDEAPADSSVEIAASLAAWFSRAKNSG
                     KVQVDYTEKKNVRSIPGSAIAHVTYVHPKTILVSPERWKEFPEAARSRQLEDRM"
     gene            2381..2455
                     /locus_tag="P5219_04425"
     tRNA            2381..2455
                     /locus_tag="P5219_04425"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2413..2415,aa:Ala,seq:cgc)
     gene            2497..2574
                     /locus_tag="P5219_04430"
     tRNA            2497..2574
                     /locus_tag="P5219_04430"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2532..2534,aa:Pro,seq:tgg)
     gene            2587..2663
                     /locus_tag="P5219_04435"
     tRNA            2587..2663
                     /locus_tag="P5219_04435"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2621..2623,aa:Gly,seq:tcc)
     gene            2672..2748
                     /locus_tag="P5219_04440"
     tRNA            2672..2748
                     /locus_tag="P5219_04440"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2706..2708,aa:Arg,seq:tct)
BASE COUNT          527 a          800 c          780 g          642 t
ORIGIN      
        1 tcgacaagcc gagcggtgtg gtggtccatc ccgcaccggg taaccgtcac ggaacccttg
       61 tccatggtct gctccatcgt ttccgccatt tcggctcctt caacaacgtc ctccgcccgg
      121 ggatagtgca ccggctcgac gcatccacat cagggctgct cgtggtcgcc agggaacagg
      181 aaatactgga gaacctccag aaccagttca ggctgaggga agtccggaaa cagtaccttg
      241 cccttgtgca cggcagggta aaaaatgctt cgggtcgcat cgatgccccc atcgggcggt
      301 gcccgacaaa ccggctccgc atggctgtgg ttgaagacgg aaaacctgct gtcaccggtt
      361 tccggagatt gtgggtccgc aacggctatt cctttctcga gtgtgacatc gccaccggca
      421 gaacgcacca gatacgggtc cacctgagtt ccatgggaca ccctatcgtg ggggacgtac
      481 tgtatggtgc cgacaaaaaa aaggcagctt ccgcgggcag ggttttcctg cactcatgga
      541 agctgtcttt ccttcatccg aaaacggggg agcggctctt cttcacatcc tgtcttccag
      601 ctgccttgac cgggcagctt cgggaaattc tttccacctc tccggactga caaggattgt
      661 ctttggatgg acgtaggtca cgtgggcgat ggcgcttccg gggatggacc ggacgttttt
      721 cttttccgtg tagtcaacct gcacttttcc ggaattcttc gccctgctga accatgccgc
      781 cagtgatgcc gcaatttcca cggaggaatc cgccggtgct tcgtcttttc ccgccgtctt
      841 gagaatgacg tgggctcccg gtatttcgtg gacgtggaac cagaggtcgg tcggagcggc
      901 gactttgaat gtgacatgcc ggtttccccg ggcgttcagg ccgatataaa tgaggtcccc
      961 gccgttttca agccggatgt ggggcggagg ggccgtcttg tgcttccggg cggacgtcgt
     1021 ttttttcggg acgagccatt ccataacatc ccgggccgat accgaaagaa ggtccgggtc
     1081 atcgatggat tccagtgttt ccacctgttc ttcaagctcg cttatgccct cttcgatgga
     1141 cagtatggcc ttcctcaccg catccctgtc gactttgccc tttttgtatt tccggaaata
     1201 cttctcggcg ttccgggaag ccgtgaggtt ctcatcgaga ggaaccagga gaggctcccc
     1261 ggtttcccag tcctgcagtt caactgccct agcccgggga ggtaccaggt cgcctgcggc
     1321 aagaagcaga ttgcccatct tcaggaactc gtttccccgt tctgaaaggc agatttggtt
     1381 ctcaaggccg tccctgtgcc gttttctgct tttgatttcc ctttcaaggg cctttttcac
     1441 tccggaaaga atgcgttctc tctgtccgag aaaataggaa gagagcactt cctcaccgca
     1501 ccgctcaaga agggggcccg gtagcacctc gcaggaaggg agcggttcgg gaaaaacagt
     1561 aaggagactt ccctgccgca aaatgaggag tccctgttct tccccgctga gagtccgcgc
     1621 caccatgtcg ttccagtgcc gcaccgggcg ggtgtcccag aaacgggtca ggatttctga
     1681 gagtcccctt cctatgcccc tgagtcccct gagatcttcc ggtccctcaa tctctcctgt
     1741 ttccccgagg tatttccctt tcaggggcgg cgggggagtg taggcaatcc ctggaaggat
     1801 catcctgtac ctgttcatgt ctccgtggat gtgcttcgcc ggttcgagga tggatcctga
     1861 ttcgtccagg atgaccaggt tgctgtttcg ctccgttcct tcgaagacaa gactcagggg
     1921 aactgaaaaa ccggctccca ccagccgttc agcggcaaaa tggaggacgc ggtcgctgtt
     1981 cagctggcgt accgaaagaa tcgaagcgtt ctggaagttt cttttcaggg cctcgccgaa
     2041 agaagaccgg gagtcccgcg tttttttcag ggaggcgata ccgtcctctc caacgagccc
     2101 gcagccgtaa ctccccgggt tccatgaaaa gagaagccac cggtcttttc cggaagaaag
     2161 gcggaaagcc gcccaggtat ctcctgtttc tatttttcct atcctgcaag gaaggactgt
     2221 ttctccgatg gagcgcgcta gcgccgtaaa aagttccggc ccgatggaca cggtgcatca
     2281 cctatcccga ctgttccgat tttaaagtct tagattatct caccttacga cgcttgacaa
     2341 gacgccttcc gctcattaca ataatcgccg ttggtctgcc ggggctgtag cgcagaggga
     2401 gcgcgcttcc ttcgcacgga agaggccggg ggttcgaatc cccccagctc caccagattc
     2461 tgttgttgcg gtttcggtaa aagttggtta caatatcggg gcgtagcgca gtctggttag
     2521 cgcacctgct ttgggagcag ggggtcgaag gttcaaatcc tttcgccccg accaagacgc
     2581 aaaggtgcgg gaatagctca gttggctaga gcgatggcct tccaagccgt aggtcgcggg
     2641 ttcgaatccc gtttcccgct ccactattca ggcgcctgta gctcagccgg acagagcaac
     2701 ggccttctaa gccgtgggtc gcgggttcga atcctgccgg gcgcgccag
//
LOCUS       DAOTHD010000197         2748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_43657_length_2748_cov_3.369848, whole genome shotgun sequence.
ACCESSION   DAOTHD010000197 DAOTHD010000000
VERSION     DAOTHD010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43657_length_2748_cov_3.369848"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(288..1337)
                     /locus_tag="P5219_04445"
     CDS             complement(288..1337)
                     /locus_tag="P5219_04445"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRX26034.1"
                     /translation="MDTSGTSFLFKCDREFNILHTFWYHPVHLLSPFQKVLTALFSPA
                     DMERIAGMAQKALSGKNLLLCEGGFSIASPEAGVCLCVTATGDTILIHGHDDYLRDGD
                     PAAAVLRDTIHRFMDVVKASDTDFMTGSDTVIREQFEKIQKLNSDLVNTQRQLKKANS
                     ALNRLNQDLNNRLVRDALTGLVSRYQYRQEIELAISRAPEKRGIFTFVDLDDFKRIND
                     TYGHAAGDRYLKEFADRLGRLPFDSLICMRIAGDEFGIYIHGYGTVEKEDIRAVWDRM
                     KELVMNRPVQLGDDVQEKIFCSAGMAVYGRDTENVYELIECADFAMYEAKKSGKNSFS
                     EFDPERYRKAKASPD"
     gene            complement(1360..2436)
                     /locus_tag="P5219_04450"
     CDS             complement(1360..2436)
                     /locus_tag="P5219_04450"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRX26035.1"
                     /translation="MSLLMNYEDQRFTRIAQMVFEEQLRRDPGLESQMDERRRKMMYD
                     DVVYNLSFLMTSVYFSDGRIFEGYARWLYELLCNLMKDLDRDRIMEIMTGHYRIMSEI
                     LADHAPEILTGDELGKAAEYLDLAIAVTTEAVTDIELSTSFSEGDYYDIRKAYLDALL
                     RSRTKNAHEIIRDARKQGVPLEHIYEKILRKVMHEIGELWHRNVITVDREHYATSVTQ
                     TVMSGFFDEIFESPRKNRTLIACAVGSELHEMGIRMLSDMFEYQGWDTFYLGAALPGQ
                     AVVEAIGEHKPDLVALSVTMPPYLSSCEGVVKAIRAAHPGVKIAVGGQAFDTTDELWK
                     KWDIDFYSPSAGELLRWAHKTFAA"
     gene            complement(2515..>2748)
                     /locus_tag="P5219_04455"
     CDS             complement(2515..>2748)
                     /locus_tag="P5219_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HRX26036.1"
                     /translation="PDGLSGDEIPLMSRIIAICDSYDAITNDRPYRKAQSRDCALGEI
                     AACAGTQFDPGLAAQFIRLFSPEEKSGTFCSGL"
BASE COUNT          527 a          770 c          772 g          679 t
ORIGIN      
        1 ctggccagca tctcggcttc ttctttttgg gagcctttgc cgtggacgta gagaaacagc
       61 ctgtcggatg gtgctccgaa gagcagcgca ggaatggtgc cgatggtttg tttttcggta
      121 atcattgaat tcctcgattc cgggatgtga tgatggcggg cattgtgtcc gcccgatttt
      181 ccgcccgtct gtatcctgcg ggattggcgt cgggtatcag cctcgggatg gccatgcaga
      241 gatcatcgga gggagctgcg ctccgtgccg cttcagaacg ggaaaaatca gtccggagag
      301 gccttcgctt tcctgtagcg ttccggatcg aattcactga acgagttctt ccccgatttc
      361 ttggcttcat acatggcaaa gtcggcacac tcgatgagtt catagacgtt ttccgtgtcc
      421 ctgccgtaca cggccatgcc cgcgctgcag aagatctttt cctgaacgtc gtcacccagc
      481 tggacgggac ggttcatgac cagctctttc atcctgtccc aaacggcccg gatatcctct
      541 ttttcaacgg tgccgtaccc gtggatatag atcccgaact cgtcgccggc gattctcatg
      601 cagataaggc tgtcgaaggg cagacggccc aggcggtccg caaattcctt caggtaccgg
      661 tcgccggcgg cgtggccgta ggtgtcgttt atacgcttga agtcgtcgag atccacgaaa
      721 gtgaagattc cacgtttttc gggggcccgg gagattgcga gttcaatctc ctgcctgtac
      781 tggtacctgc tgacaagacc cgtgagggca tccctgacga gcctgttgtt gaggtcctgg
      841 ttcagcctgt tcagagcgga gttggctttt ttgagctgcc gttgggtgtt caccaggtcg
      901 ctgttcagct tctggatctt ttcgaactgc tcacggatga cagtatcgct tcccgtcatg
      961 aaatcggtgt ccgacgcttt caccacgtcc ataaaccggt gtatggtgtc ccgcaggacg
     1021 gccgccgcag ggtcgccgtc gcggagataa tcatcatgtc cgtggatgag gattgtgtcg
     1081 cccgtcgccg tcacgcagag gcagaccccg gcttccggag aggcgatgct gaaaccgccc
     1141 tcgcacaaaa ggagattctt tccggagagg gccttttggg ccattccggc gattctttcc
     1201 atgtcggcgg gggaaaacag cgccgtcaat accttctgga agggagaaag aaggtggacg
     1261 gggtggtacc agaaggtgtg gaggatattg aattcgcggt cacacttgaa caaaaaggag
     1321 gttccgcttg tgtccattcc ggtttccttt ccggacccgt tatgcggcaa aagtcttgtg
     1381 tgcccagcga aggagctctc ctgccgaagg cgaatagaaa tcgatatccc attttttcca
     1441 cagttcgtcc gtcgtatcga aagcctggcc gcccaccgcg atttttaccc cgggatgggc
     1501 tgccctgatg gccttcacca ctccttcgca ggaagaaagg tagggcggca tcgtcacgga
     1561 aagtgccacg aggtcgggtt tgtgctcgcc tattgcctcg acgacggcct gcccgggcag
     1621 ggcggctccc aggtagaagg tgtcccatcc ctggtactcg aacatgtcgg acagcatcct
     1681 gattcccatt tcatggagct cgctcccgac cgcacaggca atcagcgtcc tgttcttccg
     1741 ggggctttcg aaaatctcat cgaagaaacc ggacatgacg gtctgggtca ccgatgtggc
     1801 ataatgctcc ctgtccaccg ttatgacgtt ccggtgccat aattcgccta tttcgtgcat
     1861 gaccttcctg aggatctttt cgtagatgtg ctccaggggc actccctgtt tccgggcgtc
     1921 cctgatgatt tcgtgagcgt tttttgtccg gcttcgcaga agggcgtcca ggtaggcttt
     1981 tcggatgtcg tagtaatcac cttcgctgaa agatgtggac agttcgatgt ctgttacggc
     2041 ttcggtagtc acagcgattg ccaggtcaag atattccgcc gcttttccca gctcgtcccc
     2101 ggtgaggatt tccggggcat ggtcggccag gatttccgac atgatcctgt agtggccggt
     2161 catgatctcc atgatcctgt ccctgtcaag gtctttcatc aggttgcaca gaagctcgta
     2221 gagccacctg gcatatcctt cgaaaatcct gccgtcgctg aaatacaccg aggtcatcag
     2281 aaagctgagg ttgtagacca catcgtcgta catcatcttt cgtcgtctct cgtccatctg
     2341 gctttcaagc ccggggtccc gtctgagctg ctcttcgaag accatctggg caattctggt
     2401 gaacctctga tcttcatagt tcatcaagag actcattttt ccatccccct gtttttaatc
     2461 aaaacggtcc ggaaaaaccc gcattgccct gtgagcgcaa agaggacgtc actttcacag
     2521 cccggaacag aaggtacccg atttctcctc tggggaaaac agcctgatga actgcgccgc
     2581 caatcccggg tcaaactgcg ttcccgcaca ggcggctatt tcaccgaggg cgcagtcgcg
     2641 gctctgcgcc tttctgtaag ggcggtcatt ggtgatggcg tcgtagctgt cgcaaatggc
     2701 gataatccgc gacatcaagg ggatctcgtc ccccgacagt ccgtcggg
//
LOCUS       DAOTHD010000198         2745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_43721_length_2745_cov_3.715242, whole genome shotgun sequence.
ACCESSION   DAOTHD010000198 DAOTHD010000000
VERSION     DAOTHD010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43721_length_2745_cov_3.715242"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="P5219_04460"
     CDS             complement(<1..354)
                     /locus_tag="P5219_04460"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HRX26037.1"
                     /translation="MRSEMLSQEKNVLSIKVEFEPGEFAKNVDKAVKELASKVNVPGF
                     RKGHAPRKILEMRFGRQAIFAEALEAMLPDAIEEIVKEYELDLIDEPSVKIEKMEEGS
                     PVEVVLTFEVTPEITL"
     gene            complement(595..798)
                     /locus_tag="P5219_04465"
     CDS             complement(595..798)
                     /locus_tag="P5219_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRX26038.1"
                     /translation="MTTQGTVKWFNATKGYGFLTSDDGRDVFVHYSAIQGDGFKTLDE
                     GQRVSFQITQGSKGDQAADVVKL"
     gene            complement(930..2288)
                     /locus_tag="P5219_04470"
     CDS             complement(930..2288)
                     /locus_tag="P5219_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HRX26039.1"
                     /translation="MKITCVINGTLRVLDARPLDRLLDILRNLGLTGAKEGCGEGECG
                     ACAVLLDGELVNSCMVPAIQVHGRTVLTAEGLGAPENPDILQKAFLEEGAVQCGFCIP
                     GMVMASRALLARIPHPDRPAIRTGLAGNLCRCTGYEKIIRAVEKAAGMGYGEGIPPFA
                     GPVCDYPPEFSDEERKSFFQPSSIAEAMEIRAEYGDEITFLAGTTDFFPDLKNGKVEV
                     KKVLDLSRVEELRAITRKDGKLFVGACASDSMIMEHPDVLSLFPALARAAELSGARAV
                     QNRATLGGNLASASGAADLPVPLLVLGASVILGSLRGERLVPIEEFFAGYRQTVLKND
                     EMLLGVSLSIPPATLRQAFYKRGSRAALTLSRISVACSAVLEEGTLKDVRIAAGSMSA
                     FPVRLPETEKSLSGKRLSAELADDAADSASREINPRKSGEFRKTVTGNLVRKFLLSLL
                     QN"
     gene            complement(2285..>2745)
                     /locus_tag="P5219_04475"
     CDS             complement(2285..>2745)
                     /locus_tag="P5219_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRX26040.1"
                     /translation="WIADVVEVEVDMDTFEIMPRKAYISAEMGKAISPVLAEGQIEGG
                     SLQAFGYAYLEDVGMKNGSYTAAHLNQYLIPTTLDTPDFSVDLQEVPFSWGPYGAKGL
                     GELPMDSGAPALVAAVGHAAGVFPEEIPVAGEYLHSLLRGKKQERKAGEK"
BASE COUNT          580 a          817 c          725 g          623 t
ORIGIN      
        1 aagggtgatc tccggggtca cctcgaaggt cagcaccact tccacggggc tgccctcctc
       61 cattttctca atctttaccg acggctcgtc aattaggtca agctcatact ccttcacgat
      121 ttcttctata gcgtcgggga gcattgcctc cagagcctcg gcgaatatgg cctgcctgcc
      181 gaaccgcatc tcaagaattt ttctgggggc atgccctttc ctgaatccag gaacgttcac
      241 cttggacgca agctctttaa ctgccttgtc aacatttttg gcgaattcac ccggttcaaa
      301 ctccaccttg atggagagaa cgtttttttc ctgggaaagc atctcggatc tcatactgtc
      361 gcaacccctt tcagcactga ttcgtactgt tgtgatatct gctcgaactg catgtatggt
      421 ccatctcttc ttcaaagaaa gagaaaaaaa tatgcggaag gtattttaca cggaaaaagg
      481 gccgaatgca aaagaaagga gattttcccg tacaaaacca ccactgtgtc aaaaaacaag
      541 aaaaaggcag cggaattaac cgctgcctta tgggtctgct cttttgagaa agggctagag
      601 ctttacaacg tcagcagcct gatctccctt ggatccctgg gtgatctgga aagacacccg
      661 ctgaccttcg tcaagagtct tgaatccgtc accctggatg gcgctgtaat ggacgaaaac
      721 gtccctgcca tcgtcgctgg tgagaaatcc gtagcccttt gtcgcgttga accacttaac
      781 tgttccctgg gttgtcaaat ttgttcctcc tagatgtgtt cactttttgc tgacggcgcc
      841 tgtcaaccag aagataataa ttggaattcc tccttctgtc aagtcggact tttccgccca
      901 aatcaacata ggtgactttc ccccgctcgt cagttctgaa ggagggacag gagaaatttc
      961 ctcacgaggt ttcccgtcac agttttccgg aattctcccg acttcctggg gttgatctcc
     1021 cgggaggcac tgtccgctgc atcgtctgcc agttcggccg acagcctttt gccggaaagg
     1081 gacttctccg tttccggaag gcggaccgga aaggcggaca tgcttccggc agctattcgt
     1141 acatccttca gagtcccttc ctccagaacg gcggaacagg cgacggaaat cctggaaagg
     1201 gtcagggctg ccctggaacc ccgcttgtaa aacgcctgac gcaaagtcgc cggaggaatg
     1261 gatagtgaca cgcccagaag catttcatca ttttttagga ccgtttgcct gtatccggca
     1321 aaaaactcct ctatggggac cagccgttca ccccggaggc tccccagaat gaccgaagcg
     1381 ccgagaacca atagcgggac aggaaggtcg gcggctcccg aggcggaggc caggttgccc
     1441 cccagagtgg ccctgttctg caccgccctg gccccactca gttcagccgc ccgggcaagg
     1501 gcggggaaaa gggagagcac gtcgggatgt tccatgatca tgctgtcgct ggcgcaggcc
     1561 ccgacgaaca gctttccgtc cttccgggtg atcgccctga gctcctccac ccgggagagg
     1621 tcgagcacct tcttcacctc aacctttccg ttcttcaggt caggaaagaa atccgtcgtt
     1681 ccggcgagga aagttatttc atcaccgtat tcggcccgta tttccatggc ttcagcgata
     1741 gaggaaggct ggaagaagga tttcctttcc tcatcggaaa actccggcgg ataatcgcac
     1801 accggtccgg cgaaaggcgg tattccttcc ccgtacccca tgccggcggc cttttccacg
     1861 gccctgatga ttttctcgta gcccgtgcag cggcacaggt tccctgcgag gcccgttctg
     1921 atcgcgggac ggtccgggtg aggaatgcgg gcgagaagag ccctggaggc catcaccatt
     1981 ccgggaatgc agaagccgca ctgtacggcc ccctcctcca ggaacgcctt ctggagtatg
     2041 tccgggttct ccggcgcccc caacccttct gcggtgagaa cggtccgtcc gtggacctgg
     2101 atcgcaggca ccatgcacga gttgacaagc tctccgtcga gaagaacggc gcatgcgccg
     2161 cactccccct ccccgcatcc ttcctttgcg cccgtcagtc caaggttccg caatatatcg
     2221 agaagccggt ccagggggcg ggcatcaagc acccggagcg tcccgttgat gacacaggtt
     2281 attttcattt ttctcctgcc ttccgttcct gcttttttcc ccggagaagg gaatgcagat
     2341 attcacctgc taccggtatt tcttcgggaa agactccagc ggcatgcccg acggcggcga
     2401 caagggccgg agcgccggaa tccatgggaa gctcgccgag tcccttcgct ccgtaaggtc
     2461 cccatgagaa gggcacttcc tggagatcca cggaaaagtc cggcgtatca agagtcgtcg
     2521 ggataaggta ctggttgagg tgcgccgctg tatagcttcc gtttttcatc cccacatcct
     2581 cgaggtacgc gtagccgaaa gcctggaggg atcctccttc gatctggcct tcagcgagga
     2641 cgggggaaat tgccttcccc atctcggctg agatataggc cttccggggc attatttcga
     2701 aggtgtccat gtccacctcg acctccacca cgtcggcgat ccagg
//
LOCUS       DAOTHD010000199         2743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_43770_length_2743_cov_2.791295, whole genome shotgun sequence.
ACCESSION   DAOTHD010000199 DAOTHD010000000
VERSION     DAOTHD010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43770_length_2743_cov_2.791295"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="P5219_04480"
     CDS             complement(<1..189)
                     /locus_tag="P5219_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26041.1"
                     /translation="MSLRRLTGMACLIGGAALLAWMAFEYFFLAFLAGKLLPEAGFLG
                     WLAGFGRVFLSLPGAAGFF"
     gene            complement(180..1427)
                     /locus_tag="P5219_04485"
     CDS             complement(180..1427)
                     /locus_tag="P5219_04485"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HRX26042.1"
                     /translation="MRLTAAVVLLLLCIGGAAWGAAHPLDGEIEKCMNDDPSTQGTIQ
                     CANMGKKKWDGELNRAYNELMKLLPKEGQSLLRTAQRAWIPWRESEYKLLGGVYLTIY
                     NNLDGGTMWLVADAIAEMEVVRERALELIAYTEEFKAGKPSFSGSYPKGQTEEQLTAA
                     MKVKNDHKKLGRAFGSNGEVIAAEALQSWEDFRNKEAAFMTWFYGKTGDTAFPLHARM
                     LKNTARLKKLEGLYDDLETGGLKESPSGETAKKSGGGKEPFAGDRSLYRPSEGTCDFG
                     AYIIDPDPKGTNVRETPNGKVTAVLPHQPDDPALIMVKVTGYRDKWLSVVLHDGTKGW
                     IFSELAGMSLRNYAPGSVAVLRTRPDENAPAVGDIFGDEEVTVLGGEGAWALVRYRHP
                     KGHEFIGWLDPEKQCDNPYTTCP"
     gene            complement(1442..2176)
                     /locus_tag="P5219_04490"
     CDS             complement(1442..2176)
                     /locus_tag="P5219_04490"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRX26043.1"
                     /translation="MKRMVLLAAAILAVLTAVPLFAAAAAENFEWPKAGFVTGSRVNL
                     RNGPSTSAKVVGRFGHDYETGELVVTGTEKTGEEFPWYRVLSHQFGEGWIYGKFLSVE
                     ETDSTVRRYVMKIREDFGISPALAVKMYGEPMKREERKVKLPDFNATVHIVDLFFHGH
                     NATYWNGALQAVDIPAGFMGFGDIVMGMDAGEALSRLGAPSEKESPERYFYIHERDEI
                     DIMFDRDPEDGGGRVTGLHYRRVVYD"
     gene            complement(2202..>2743)
                     /locus_tag="P5219_04495"
     CDS             complement(2202..>2743)
                     /locus_tag="P5219_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26044.1"
                     /translation="RILCRACCLMVLFLSAALAVQSEGALPRTAVEFEMRYRQEGTTP
                     EAAAKLFFDGIFAYMDRSTREEGRKMLALAMDERPDWDGRVTMKLFADRMKSPKTAHI
                     FRSYARGAVPENGYAMDPDNYELVIERSAEGHPKGLQLYLRSGGADYPRVIYMKEVNG
                     FWFIADGSTVKVEVRPPRK"
BASE COUNT          480 a          888 c          779 g          596 t
ORIGIN      
        1 gaaaaagccc gccgctcccg gcagggagag aaacaccctg ccgaaccccg caagccagcc
       61 gagaaaaccg gcttcaggca gcagttttcc ggcgaggaag gcaaggaaaa agtattcgaa
      121 agccatccac gccagcaggg ccgctccgcc gatcagacag gccatgcccg tcaggcgtct
      181 cagggacatg tggtgtaggg gttgtcgcac tgcttctccg ggtccagcca gccgatgaac
      241 tcgtggccct tcggatgcct gtaccggacg agagcccatg caccttcgcc gccgaggacc
      301 gtcacttcct cgtcgccgaa gatgtcgcca acagcggggg cgttttcgtc gggccgtgtt
      361 ctcaggacgg ccacggagcc cggcgcgtag ttgcggaggc tcattcccgc cagttcgctg
      421 aagatccatc cctttgtccc gtcgtggagg accaccgaaa gccatttgtc cctgtatccc
      481 gtaaccttca ccatgatgag ggccggatcg tcgggttggt gaggcaggac ggcggttacc
      541 ttgccgttgg gggtctcccg cacgttggtt cccttgggat cgggatcgat gatataggcc
      601 ccgaagtcgc aggttccttc ggaaggacgg tacaggctcc ggtccccggc aaagggttcc
      661 tttcctcctc cggatttctt cgcggtctcc ccggacgggg attctttcag gccgccggtt
      721 tccagatcgt cgtagagacc ttcgagtttt ttgagccggg cggtgttctt cagcatcctg
      781 gcatgaaggg gaaaggccgt gtcgcctgtt ttgccgtaga accacgtcat gaaggccgct
      841 tccttgttcc tgaagtcctc ccacgactgc agggcctcgg ccgcgatgac ctcgccgttg
      901 gagccgaagg ccctgccgag ttttttatga tcgttcttta ccttcatggc agcggtcagc
      961 tgctcctccg tctgcccctt ggggtagctt ccggaaaaag acggttttcc tgccttgaac
     1021 tcttccgtgt aggcgatcag ttccagtgcc ctctccctga ccacctccat ctcggcgatg
     1081 gcgtcggcca caagccacat ggtccccccg tcgaggttgt tgtaaattgt gagatacacc
     1141 ccgccgagga gcttgtattc gctctccctc cagggaatcc aggctctctg ggcagtccgg
     1201 agaagtgact gtccttcctt cgggagaagt ttcatgagtt cgttgtaggc ccggttgagt
     1261 tccccgtccc atttcttctt ccccatgttt gcgcactgaa tggtcccctg ggtgcttgga
     1321 tcgtcgttca tgcacttctc gatctccccg tccagcggat gggccgctcc ccaggccgcc
     1381 ccgccgatac agagcagaag gaggaccacg gcggcagtca gtctcattgt cataccccct
     1441 gtcaatcgta gacgaccctg cggtagtgga ggcccgtcac cctgccgcct ccatcctccg
     1501 ggtccctgtc gaacatgata tcgatttcat cccgttcgtg gatgtaaaaa taacgctccg
     1561 gggactcctt ttccgacgga gccccaagcc gggagagagc ctcgccggca tccatgccca
     1621 tgacgatgtc cccgaaaccc atgaaacccg cgggaatatc caccgcctgg agagcgccgt
     1681 tccagtacgt ggcgttatgg ccgtggaaga aaaggtccac gatatggact gtggcgttga
     1741 aatcgggaag cttcaccttt cgttcctccc gcttcatggg ttcgccgtac atcttcaccg
     1801 ccagcgcagg ggaaatgccg aaatcctccc ggattttcat gacgtagcgc cggacggtgc
     1861 tgtccgtctc ctcgacggaa aggaacttgc cgtagatcca cccctcaccg aactggtggg
     1921 aaagcacccg gtaccacgga aactcctctc ccgttttctc tgtgccggtc accacgagtt
     1981 cccctgtttc gtagtcgtgg ccgaaccgtc ccacaacctt cgccgatgtg gaggggccat
     2041 tccgcaggtt gaccctcgat ccggtgacga atcccgcctt cggccattcg aaattttcgg
     2101 ccgcagcggc ggcgaagagg ggaacagccg tgagaaccgc caggattgcc gccgcgagca
     2161 agaccattcg cttcatcatc ccgtcctcct ttccgccgct gctatttcct cggcgggcgc
     2221 acttccacct tgaccgtact tccgtcggcg atgaaccaga agccgttgac ctctttcatg
     2281 tagatcaccc ggggatagtc cgcgccgccg ctccgcaggt agagctgcag ccccttgggg
     2341 tgaccttccg ccgaccgttc gatcaccagt tcgtagttgt ccgggtccat ggcgtatccg
     2401 ttttccggta cggctcccct ggcgtagctc ctgaagatat gggcggtctt cgggctcttc
     2461 atcctgtcgg cgaagagttt catggtgacc cggccgtccc agtcgggccg ctcgtccatg
     2521 gcgagggcga gcattttccg tccctcctcc cgggtggacc tgtccatgta ggcaaagata
     2581 ccgtcgaaaa agagtttcgc cgcagcttcg ggagtcgttc cctcctgccg gtagcgcatt
     2641 tcgaactcca cggccgtcct ggggagagcc ccttcgctct gcacggccaa ggccgcggac
     2701 aggaaaagga ccatcaggca gcaggccctg cacaaaattc ttt
//
LOCUS       DAOTHD010000200         2742 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_43781_length_2742_cov_3.225158, whole genome shotgun sequence.
ACCESSION   DAOTHD010000200 DAOTHD010000000
VERSION     DAOTHD010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43781_length_2742_cov_3.225158"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..366)
                     /locus_tag="P5219_04500"
     CDS             complement(16..366)
                     /locus_tag="P5219_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HRX26045.1"
                     /translation="MEHSAGTKLVVLWTSGEKITAMNMVMLYCLNSKLKGWWDEVTLL
                     VWGASTGLLAEDGEIQTYLSTLQEAGVEVIACKKCAENLGAAEKLENLGVKVFYTGQY
                     LTEKIQSGHKILSV"
     gene            complement(586..2271)
                     /locus_tag="P5219_04505"
     CDS             complement(586..2271)
                     /locus_tag="P5219_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26046.1"
                     /translation="MAKGGTGSAYRLLLTLIFLLSFPSASFSWGAIKDYAETHQYILR
                     EAMKLAASDPAFVQKFPSAEELVNHDYVLVTALGKSGPGPDVDGASPYSWHYYNPRTG
                     KGLAPKKVADFFSVMMHKRVRGESQAREAAWAAHFLADMFVPYHVTGMPEEEAWQSFM
                     AGTPLGEKETGPAELYSMKSPPPAGWGEGGDFSSALELFFNEYPPENDEGVDWFDPWY
                     FNGWGGATAPGIATGSHGFWEYQAWEWFRVKQVVKKSLPAPGYDPLWKNRKSEMGTVF
                     WRAQAAAAAEYAAACASRTRQNIPAILSSPDQGIQNAVRAVYTLYRASMTSLRASWRQ
                     EDLTGGGYRVFCTVRNTNPLEEFAGAGARLLYRKDGRWIVRETVTLPRPVPPQGAGEF
                     FWDFRSGDSFQCIAEVAGLFRQTPDLGYVNLSFTTGGAAPEMDHGRTFAGTMNFSMAN
                     YPEGTRINQLSLSISPSGGNVRGTARLRFMPQVPGGMKPMGLELFLEGAFDGTRLSGT
                     CRGTQENFFWTGGGSAAGRWSASLSGEQVKGSIVLERDGKTYSLPFYGNEPVL"
BASE COUNT          595 a          829 c          675 g          643 t
ORIGIN      
        1 gtaagaaccg gctcttcaca ccgagagtat cttatggccc gactggattt tttctgtcag
       61 gtactgcccg gtgtaaaaaa ccttcacgcc gaggttctcg agcttctccg ccgcaccaag
      121 gttttctgcg cattttttgc atgcaatcac ctccacaccc gcttcctgaa gagtgctgag
      181 gtacgtttgt atttcaccgt cttcggcaag gagccctgtc gaggctcccc acacgagcag
      241 cgtaacctcg tcccaccatc ccttcagctt cgagttcagg cagtagagca tgaccatgtt
      301 catcgcagtt atcttctctc cgctcgtcca caggacaacc agttttgttc ctgctgaatg
      361 ttccacgctg cccctccttt ttctctttga gcaactgggt ttgtacccct aacccctgct
      421 tctggagcgg tcgccgtcaa tgaaccattc cagggcgatg ggcaggtaca gcctgtccgg
      481 gacgaccgcc ggccacggaa ggccctccgg agaatgcttc acgcccattg agcaggaaaa
      541 gaagaatcaa aacaataatt gttctcattt tctccccctc catggttaca gcaccggctc
      601 atttccgtaa aacgggagac tgtatgtttt tccgtctctc tcaaggacta tcgatccttt
      661 cacctgctct cccgagagac tggcgctcca ccttccggct gcgctgccgc cgcctgtcca
      721 gaaaaaattc tcctgcgttc cccggcaggt tcccgacagg cgcgtcccgt cgaatgcccc
      781 ctcaaggaaa agttccaggc ccatgggttt catgcctccg ggcacctgag gcataaacct
      841 gagcctggcg gttccccgaa cattcccgcc ggacggggaa atagagagag acagttgatt
      901 gatcctggtt ccttccggat aattcgccat ggaaaaattc atcgtccccg caaaggttct
      961 tccatgatcc atttccggag cggcgccgcc ggtcgtgaag gaaaggttca cataccccaa
     1021 atcgggagtc tgccggaaca gacccgcaac ctcagcaatg cactggaaag aatcacctga
     1081 acgaaaatcc cagaaaaatt ctccggctcc ctggggcggt acaggacggg gaagcgtaac
     1141 cgtctctctc acaatccatc gtccgtcttt tctgtacaac agccgcgccc cggcacccgc
     1201 aaattcttcc agtggattcg tattgcgcac cgtgcagaaa accctgtatc ctcccccagt
     1261 aagatcttcc tgccgccagg atgcccggag ggatgtcatg gaagccctgt aaagggtata
     1321 cacggcacgg acagcattct gtatcccctg gtccggactg gacagaatgg ccgggatgtt
     1381 ttgccgggtt cgtgacgcgc atgcggcagc gtattctgcc gcagcggcag cctgggctct
     1441 ccagaaaacg gttcccattt cggatttccg gtttttccac aacgggtcgt acccgggggc
     1501 gggcaggctt tttttcacga cttgctttac cctgaaccat tcccatgcct ggtactccca
     1561 gaatccgtgg ctgcccgtgg cgatgccggg agcagtcgct ccgccccatc cgttgaaata
     1621 ccacggatcg aaccagtcca ctccctcgtc gttctcaggc gggtattcat taaaaaacag
     1681 ctccagggct gaagaaaagt ctcctccttc tccccatccc gccggcggcg gactcttcat
     1741 cgaatacagt tccgccggcc ccgtctcctt ttccccaagc ggcgttcccg ccatgaagga
     1801 ttgccatgcc tcctcttccg gcattcccgt cacatggtac ggaacgaaca tgtcggcgag
     1861 gaaatgagcc gcccaggcgg cttccctggc ctgagattct cccctgaccc ttttgtgcat
     1921 catgacgctg aaaaaatccg ccactttctt cggggcaagg cctttccctg tcctcgggtt
     1981 gtaataatgc cacgaatagg gtgaggcccc gtccacatca gggccgggac cggatttccc
     2041 aagggctgtc acgaggacat aatcatggtt tacgagttct tccgcagaag gaaacttctg
     2101 cacaaacgcc ggatcggaag ctgccagttt catcgcttcc ctgagaatgt actggtgtgt
     2161 ttccgcataa tctttgatgg ccccccagga aaaacttgcc gaagggaagg agaggagaaa
     2221 tatcaatgtc agcaataacc tgtatgctga tccggttccg ccctttgcca tgggattatt
     2281 accctccttc gccggagact gattcagaca aaagccaatg actgattgtt ctgttttttc
     2341 acagtataga tggtgtgaaa aggcgggtca attgattctt tcccgggaaa caattctttc
     2401 ccgaaaatcg aaaatctctc gaggtttttg ctcaagacac aggctacatt atctctagtc
     2461 tccagttggg atggacagga gagaccgtac gttccgcatg atagacaaag cgtatcctgc
     2521 tgccggggtg caatgatttc tggccgaaca cgtacccgcc gaaagcgggt atggagtcca
     2581 caatgtgaaa cgttctgaaa acggagacat actgctccgc ccaggccttc ccgcaccgtc
     2641 ggtggtgttc caggttgtca gggtaattca cgacctggaa gtaaaaatca cccctggttt
     2701 cttcatgcgg tctgccctct acattgatgg cgccggtaaa aa
//
LOCUS       DAOTHD010000201         2738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_43879_length_2738_cov_3.141633, whole genome shotgun sequence.
ACCESSION   DAOTHD010000201 DAOTHD010000000
VERSION     DAOTHD010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43879_length_2738_cov_3.141633"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..1220
                     /locus_tag="P5219_04510"
     CDS             189..1220
                     /locus_tag="P5219_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26047.1"
                     /translation="MEKTETLAADAGTGIDTIKGVFARYPDMPESAKKTLSDRILALA
                     KNEASKIAAEIEAAGSLEALGARLPDLKLPWTDYPAGKDDVAESFKKTFSRLVASEWG
                     KIAEKGSALAEKKDFAGAKELFASSVEALTARLEKAGLGAFGNGAVAEAAEVQGKKID
                     ELRQSHFSACKSAFEGLKSTKSKDDILPVAETLRAFTVLWPDSEEAAEAGKAVLFLDA
                     VQNGTKAVLTIVGGDFSAVDSLLDTPDMKILLKMDGKTVLETKTVDNEVRPSFGEKHE
                     FTWDIGSRFTLVGMETGGILGSDKEVFNVSVDAGGILGYKKLTGTLKNNGNSITVKLD
                     IELPDSPWN"
     gene            complement(1309..2316)
                     /gene="dctP"
                     /locus_tag="P5219_04515"
     CDS             complement(1309..2316)
                     /gene="dctP"
                     /locus_tag="P5219_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRX26048.1"
                     /translation="MRKVVLSVLLGVLFFIPAFFSGCAAAAPEMTLRFSGQFPEGHTA
                     TMLMRQVAEEVLAKTGGRIEIKVFPDNELGDYTLVYEELIRGSIDMAAITVPSQFDPR
                     LELAYINCIVRTFDGARKLFAPDGWLFKKMDGLHSRLGVKLLAFQMEGFIGIGSTKPV
                     NEPLNPGVKKGILLRIPNMEVFKLGAEAMGYRTITIPWDEVRGSLQNGFAEGVNGMTP
                     TAAYSMLKGVLKYWYDLRYSMENLNYMISLKTWEKLKEEDRAVIADACSKITATSIDL
                     AEKDQQKHIDLLREAGVEVHTYTMDELAPVFSKVSATWDRLADKLSAELIDEFIKEYA
                     E"
BASE COUNT          674 a          756 c          706 g          602 t
ORIGIN      
        1 gcgtcgcctc ggcgaatcgg tccgttgcag caacatcggc gaacccgatg acgtgatcca
       61 gaatgaagcg ctggtcggcc agggcggctt tggtcatcgg aactctccgg cttgtctcga
      121 gagcgtggct ctcctatgag gcaaggctag ccaagtcgcc ccttcctgca agctggacgc
      181 tgcgtcagat ggaaaagacc gaaacccttg ccgccgacgc cggcacaggc atcgatacca
      241 tcaagggtgt ttttgcccgt tacccggaca tgccggagtc agcaaagaaa acactctctg
      301 accgaattct cgccctggca aaaaatgaag cctcaaaaat tgccgccgaa atagaggccg
      361 ccggctccct ggaagccctt ggggccagac tgcctgacct gaagctcccc tggacggact
      421 accccgccgg aaaggacgat gtggcagaaa gtttcaaaaa gaccttctcc aggctcgtag
      481 cttcggaatg ggggaaaatc gctgaaaaag ggtccgctct tgcggaaaag aaagattttg
      541 caggagcaaa ggaactgttt gcctcctccg tggaagccct cacagcaaga cttgaaaagg
      601 ccgggctcgg tgccttcgga aacggtgcgg tagccgaagc ggctgaagtc cagggtaaaa
      661 aaatcgacga actgcgtcaa tcccactttt ctgcctgcaa atcagctttt gaagggctca
      721 aatccaccaa aagcaaagat gacattctcc cggtcgcaga aacgctgaga gcgttcaccg
      781 ttctctggcc tgattcggaa gaagcagcgg aagccgggaa ggcggtcctg ttccttgacg
      841 cagtacaaaa cgggaccaaa gccgtgctga ctattgttgg cggcgatttt tccgcagttg
      901 attcgctgct cgatactccc gatatgaaga tcctcctgaa aatggacggc aagactgtgc
      961 tggagaccaa aacggtggat aacgaagtac gtccctcttt cggcgaaaag catgaattca
     1021 cctgggacat cggatcccgc ttcacccttg tgggaatgga aacagggggc atcctgggtt
     1081 cggacaagga agtattcaac gtctcagtgg acgccggcgg aattctcggc tacaaaaagc
     1141 tcaccgggac cctcaagaac aacgggaatt ccattaccgt caagctggac atcgaacttc
     1201 cggattcacc ctggaactga tctttcgact ttcccggaca gcgaaaaagg acggaacccc
     1261 gacaacgggt tccgtccttc tcttatccac caggttctaa aaaaattact attcggcgta
     1321 ttccttgatg aattcgtcga taagctccgc agacagctta tctgccagcc tgtcccatgt
     1381 ggccgatacc ttggaaaaaa cgggggcgag ctcatccatc gtataggtat ggacttcaac
     1441 tccggcttcc ctcaggagat caatatgctt ctgctggtcc ttttcggcga gatcgatgct
     1501 ggttgcggtg atcttcgaac aggcatccgc gatgacggca cggtcctctt ccttcagctt
     1561 ttcccaggtc ttcaggctga tcatgtagtt caggttttcc atggaatacc ggagatcgta
     1621 ccagtatttc agaaccccct tcagcattga gtaggccgct gtcggcgtca tgccgttcac
     1681 accttccgcg aaaccgttct ggagggatcc ccgcacttcg tcccatggga tggttatggt
     1741 cctgtatccc atggcttctg caccgagctt gaaaacctcc atgttcggga tgcggagcag
     1801 aatgcccttc ttcaccccgg gatttagggg ctcatttacg ggtttcgtgc tcccgatgcc
     1861 gataaagcct tccatctgga aagcgagaag cttcacacca agacgggaat gaaggccatc
     1921 catctttttg aaaagccacc cgtcaggggc gaaaagcttc ctggcaccgt caaaagtacg
     1981 cacaatgcag ttgatatagg ccagttcgag cctcggatcg aactggctcg gaacggtgat
     2041 tgccgccatg tcgatggatc cccggatcaa ttcttcgtaa actagtgtgt aatccccaag
     2101 ttcgttgtca gggaaaacct tgatttcaat gcgccccccg gtcttcgcca ggacttcctc
     2161 cgcaacctga cgcatgagca tggttgcagt atggccttca ggaaactgac cggaaaagcg
     2221 gagcgtcatt tccggcgccg cagccgcgca tccggaaaag aaagccggaa tgaagaaaag
     2281 gacaccaaga agaacggaca ggacaacctt tcgcatcaaa cgcactcctt tcagaaatga
     2341 acccttcgcc ttgctttcat gaccgcgccg tctccgcagg gaccggtaca tctttcctaa
     2401 gggtaataat acaacatttg acctctctgg agagggcatt tttcatttct gaaggataga
     2461 tagatcaggc tccatggtca attttcggcg tgaaagtgaa ttccttgagc cttcagctga
     2521 tgctgaactc tacccgccgg cgagtagagt tcagaaccaa atgtttttcc tctttctgtg
     2581 ctctcttcag gctaaataca ggtacaatct caatagaaaa cataaaaaac tggagtaagg
     2641 ggatttgatc cccctttatc tgcaccgcca cctgcgggaa cctgtgaaca tgcgggaaga
     2701 gcaatggccg ttcggcttca cccggtcaaa ccggcatc
//
LOCUS       DAOTHD010000202         2735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_43959_length_2735_cov_2.897761, whole genome shotgun sequence.
ACCESSION   DAOTHD010000202 DAOTHD010000000
VERSION     DAOTHD010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43959_length_2735_cov_2.897761"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..1184)
                     /locus_tag="P5219_04520"
     CDS             complement(138..1184)
                     /locus_tag="P5219_04520"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HRX26049.1"
                     /translation="MTRFPPVLTSPSGPGWETGGLVLDSPLMLAPLAGVTIPPVRLFY
                     SRLGASATHTEMISCAGLLRTNRKTAAMLQVLPGEGPVIVQLFAGDPDTLSKGAETAL
                     AEGTLFRAAGINMACPMPKVLKKGAGARLLENPDTAFAMVKALTRFGFPVWPKIRKCP
                     PGYPLTTEEFCRGLLDAGASLVCLHGRTPAQRYAGEADKDMVARIAEMFPGKICASGD
                     VYSPRDISFYLDAGCSSVLLARGAVADPFLFARTLAFLGYDVHNIYGNPSPDFQILLL
                     LDFGDGVSALCGQRMAALFAKRILAGLFRGMAGVGGLRRAAASVSSWTDLRGIIEQSG
                     EYFERREKYSGCTV"
     gene            complement(1181..1948)
                     /locus_tag="P5219_04525"
     CDS             complement(1181..1948)
                     /locus_tag="P5219_04525"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRX26050.1"
                     /translation="MLLVLDVGNTTTVIGIYDGETLVKHWRLMSERHTSDELGIYLLN
                     LLDICGIPRTSIDGAIYSSVVPSLDMALSEGLREYLSVDPVKVTPSLDLGIEIAYSPR
                     HEVGADRLVNSVAGKARYGAPLIVVDFGTAITLDILNSEGAYLGGTISPGLVTGMEAL
                     FGRTAKLPQVSLEPPKSVIGNNTIGSIQAGILFGNAGLVDFLVRKTWDELGCRTPVAA
                     TGGHAAAIASISETITAVDPWLTLEGLRLIHERNRPR"
     gene            complement(2065..>2735)
                     /locus_tag="P5219_04530"
     CDS             complement(2065..>2735)
                     /locus_tag="P5219_04530"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRX26051.1"
                     /translation="VRVRTPAGEFAYALLAGGAPSALRAALMVQSVLLGYVLGRKGSP
                     LNAVSLAGLLLLLWRPEWYADLGWRLSVTAALLLSALAERGAGWRTALAASPLVWLAT
                     YPQTAAAFGVVPLSGIVVNMVALPVFAFLYPFAALLSIPALAGLPGGYFLAGAAEGLF
                     LLWQWAADTLTSFIPWSLSWSPAAAAAGSAAFLASLAFGLFRPGLRTFGGALLLLLAG
                     MILF"
BASE COUNT          568 a          825 c          881 g          461 t
ORIGIN      
        1 gcttccggtc ccacttttcc acgaggtagg ggttgtatcc ctcctcggcg atcagccgct
       61 cgagcttgtc cttccgctgg cggacgatct cgtcctctcc gtcgttcccc tgggggagac
      121 ggttcggggc gtccttttca tactgtgcat ccactgtatt tttctctcct ttcaaaatac
      181 tcgccgcttt gttcaataat tccccggaga tccgtccatg aggacactga tgcggcagcc
      241 cttcgaagac cgcccacacc agccatgcct cgaaacagcc cggcaagaat gcgtttagca
      301 aaaagcgccg ccatcctttg cccgcacaaa gccgatacgc cgtcgccgaa atcgagcaac
      361 aacagtatct gaaaatccgg ggatggattt ccgtagatat tatgcacgtc atagccgaga
      421 aacgccaggg tccgggcaaa aaggaaaggg tccgccacgg cacccctcgc aaggagcacg
      481 gacgaacatc cagcatcaag gtaaaaagag atgtccctgg gagaatagac atcaccgctt
      541 gcgcatattt tcccggggaa catttcagca atccgtgcga ccatatcttt gtccgcttcc
      601 ccggcatacc gctgtgcggg agtccgtccg tgaaggcaga ccagggacgc gcctgcatcc
      661 agaagccccc ggcagaactc ctccgtagtc agggggtagc cgggaggaca tttgcgtatc
      721 ttcggccata cagggaaacc gaagcgtgta agggccttca ccatggcaaa agcggtgtca
      781 gggttttcca ggaggcgggc tcccgctcct tttttcagca ccttcggcat ggggcaggcc
      841 atgtttatgc ctgcggcacg aaacagtgtc ccctccgcca gcgccgtttc agctcctttc
      901 gagagcgtat ccgggtctcc ggcaaaaagc tgcacgatca caggtccctc tccgggaagc
      961 acctgcagca tcgccgctgt tttccggttt gtcctgagca gccctgcgca gctgatcatt
     1021 tctgtatggg tcgctgaagc cccgaggcgg gaataaaaaa gccgcacggg ggggatggtg
     1081 acccctgcga gaggggcgag catcaggggg gaatcgagaa ccagcccccc agtctcccat
     1141 ccgggcccgg aaggtgacgt aagaacggga ggaaaacggg tcacctgggg cggttcctct
     1201 cgtggatgag ccggagcccc tccagggtga gccacgggtc cacggcggtg atggtttccg
     1261 agatggaggc gatggccgcc gcatggcccc cggtggcggc cacgggcgtt ctgcagccca
     1321 gctcgtccca ggtcttccgg acgagaaaat ccacgaggcc cgcgttgccg aagaggatac
     1381 ccgcctggat ggagccgatg gtgttgttcc cgatgacgct cttcggcggt tccagggaga
     1441 cctggggcag cttcgccgtg cgtccgaaga gggcttccat gcccgtcacc aggccggggg
     1501 agatggtccc gccgaggtag gcgccctcgg agttcaggat gtcgagcgta atggccgtgc
     1561 cgaagtccac cacgatgagg ggggcgccgt acctggcctt ccccgccacg gagttcacga
     1621 gccggtcggc ccccacttca tgcctgggcg aataggcgat ctcgatgccg aggtcaaggg
     1681 atggcgttac cttgaccgga tccacggaaa ggtattccct caggccttcg gaaagggcca
     1741 tatcgaggga gggaacgacg ctcgagtaaa tggccccgtc gatggacgtc cgggggatgc
     1801 cgcagatatc cagcaggttc agcaggtaaa tgccgagctc gtcggaggtg tgcctttccg
     1861 acatgagccg ccagtgcttc accagggtct ccccgtcgta gatgccgatg accgtggtgg
     1921 tgtttccgac gtcaaggacg agcagcatgg taccgtcctc ctctttgaag gatttcaaca
     1981 tcaggatatt ataccctgtc ggaagggaaa tttcgtcccc cttgcccatt ggggtgaata
     2041 ttgccggtac cggagggcgg aacatcagaa aagaatcatc ccggcgagaa gaaggagcag
     2101 ggctcccccg aaggtccgga gtcccggccg gaagagcccg aaggccaggg aggcgagaaa
     2161 ggcggcgctg cctgcggccg ctgcagcagg cgaccaggat agggaccacg ggatgaatga
     2221 cgtgagcgtg tcggcagccc actgccagag gagaaaaagc ccctcggcgg ctcctgcgag
     2281 gaaataccct cccggtagcc cggcaagggc tggaatcgaa agcagggcgg cgaaaggata
     2341 aagaaaggcg aagaccggga gggccaccat gttgaccacg atgccggaca ggggaaccac
     2401 gccgaaggct gcggcggtct gggggtaggt ggccagccag acgaggggac tcgccgcgag
     2461 ggccgtcctc cagcccgctc cccgttcggc aagggcggaa agaagaaggg ccgccgtgac
     2521 ggacagcctc catccgaggt cggcatacca ctctggtctc cagagaagga gaaggagccc
     2581 cgcaagggag acggcgttca gagggctccc cttccgccca aggacatacc cgaggagaac
     2641 ggactgcacc atcagggctg cccggagagc actgggggct cccccggcca gcagggcgta
     2701 ggcgaactcg cccgcagggg tgcggacgcg gacgg
//
LOCUS       DAOTHD010000203         2732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_44016_length_2732_cov_3.953306, whole genome shotgun sequence.
ACCESSION   DAOTHD010000203 DAOTHD010000000
VERSION     DAOTHD010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44016_length_2732_cov_3.953306"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="P5219_04535"
     CDS             <1..230
                     /locus_tag="P5219_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26052.1"
                     /translation="SLFLFFPLMGIMFYLAFTWAIRAVTIKEVFFNSFWYPPAWPYRT
                     VFAVGLFFLLLQGAANLVRDFYFMVRGETLD"
     gene            223..1566
                     /locus_tag="P5219_04540"
     CDS             223..1566
                     /locus_tag="P5219_04540"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRX26053.1"
                     /translation="MIELSAEAVTAIMLGGVFVLVMTGFPIAFVIGSVAFFTGFAVFG
                     PTTTFHILYSRFYDLSLNYPYLAVPLFTFMGVILQHSGVTKDLYQSLYEAMGRLRGGL
                     AVVTILFGTILAACLGVIAASVTILTLIALAPMVTRGYDKSLAAGSIVAAGTLGILIP
                     PSIMLVVYAPQAGLSVGQMFMGAVFPGLLLSAFYMLYVIIRCRLNPALGPAIPEDQIT
                     PFDAAKFFRLLKSLVPPILLIAAVLGTIFAGIAPPTEAAAVGCFASILLAIAYGKFSW
                     GLMKRASLETMKVSAFVVMIAAMCYAYVGIFMNAGAGDVVAEFILSVPGGKWMSFFVI
                     MAIVFLLGMFIEWIGIVFIVVPIFSPILAALGFNPLWAGMMICINMQMAFQTPPMAMS
                     IFVLKGTAPKELGVTIGDIIWGVIPFILIIMVVLVLCAVFPEIILWLPEKMIGAA"
     gene            complement(1646..>2732)
                     /locus_tag="P5219_04545"
     CDS             complement(1646..>2732)
                     /locus_tag="P5219_04545"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRX26054.1"
                     /translation="RLFGKISRNMSLFAGNILRILPPVALSLTSTQTILLVDDTINIT
                     GRLCIFLIQFFSAVRWFGWAWCAIRVGEAIAELIILSPRVEPESIDASLVRTVSRLIC
                     ITAASWILLYGLSRLGLSVFHLLTGLGVVGLAISLAAKPTVENIIGGLTLFADRSVKV
                     GEYCRFGNTAGTVLHIGLRSTKIEALDRTVISIPNAEFSQMQIVNVTRRNGSLMQKTL
                     SLRYETTGDQLRWILASVRDVLHAHPKVAADPVPFARFESYGASSLDILVFAYIRTKK
                     WAEFLAVQEDILFRIGEIVEKSGSGFAFPSTTAYIARDGGLDGEKAALAAEEVRQWRE
                     SGQYPFPDTPGERIAAARGTVEYPPKS"
BASE COUNT          470 a          829 c          824 g          609 t
ORIGIN      
        1 gctccctctt tctcttcttc ccgctcatgg gcatcatgtt ctaccttgcc ttcacgtggg
       61 ccattcgtgc cgtcaccatc aaggaagtgt tcttcaacag cttctggtat cctccggcat
      121 ggccctaccg taccgtcttc gccgtggggc tcttcttcct ccttctccag ggggctgcga
      181 acctcgtccg ggatttttat ttcatggtga ggggtgagac tcttgattga actcagcgct
      241 gaagcagtca ccgccataat gctgggagga gtcttcgtcc ttgtgatgac ggggttcccc
      301 atcgcgttcg tcatcgggag cgtggcattc ttcacggggt ttgccgtctt cggtcccacc
      361 acaacgttcc acatcctgta cagccgcttc tatgacctgt cgctgaacta tccctacctg
      421 gcggttcccc tgtttacctt catgggggtc atactgcagc attccggtgt cacgaaggac
      481 ctgtaccaga gcctgtacga ggccatgggc aggctccgtg gcggcctggc ggtggtgacc
      541 atcctcttcg gaacgatcct cgccgcctgc ctgggcgtca ttgccgcatc ggtcaccatc
      601 ctgaccctta tcgccctcgc gcccatggta accaggggat acgacaaatc ccttgcggcc
      661 gggtccatcg tggctgccgg aacactcggc atccttatcc cgccgagcat catgctcgtg
      721 gtctacgctc cccaggcggg gctgtcggta gggcagatgt tcatgggggc cgttttcccg
      781 ggattgctgc tctcggcgtt ctacatgctc tacgtgatca tccgctgccg cctgaacccg
      841 gccctcgggc cggccattcc ggaggaccag atcacgccct tcgatgcggc caagtttttc
      901 cgtcttctca agtcgctggt gccgcctatt ctcctgatcg ccgcagtgct cggcaccata
      961 ttcgccggaa tcgcgccgcc caccgaggcg gctgcagtcg ggtgcttcgc gtccatactt
     1021 ctggcaattg cctacgggaa attcagctgg ggactcatga agcgggcgtc cctggaaacc
     1081 atgaaggtca gcgccttcgt ggtgatgatc gccgccatgt gctacgccta cgtgggcatt
     1141 ttcatgaacg ccggagcagg cgacgtggtg gcggagttca ttctctccgt tccggggggg
     1201 aagtggatgt ccttcttcgt gatcatggcc atcgtgttcc tgctgggcat gtttatcgag
     1261 tggatcggca tcgtgttcat cgtggttccc attttctcgc ccatcctggc ggccctgggc
     1321 ttcaatcctc tctgggccgg catgatgatc tgcatcaaca tgcagatggc cttccagacg
     1381 ccgcccatgg ccatgtccat cttcgtgctg aaaggcacgg cgccgaaaga gcttggcgtc
     1441 accatcgggg acatcatctg gggagtcatt cccttcatcc tcatcatcat ggtggtgctg
     1501 gtgctctgtg ccgttttccc ggagataatc ctctggctgc cggaaaagat gatcggcgcg
     1561 gcctaaggca aagaaaagaa agggaaaaaa acaaaaggcg gcccggagat ttttccgggc
     1621 cgcttttgtt ccggtttctg tcccgtcagg attttggggg atactccacc gtacctcgag
     1681 cagcagctat cctctccccg ggagtgtccg ggaaagggta ttgtccgctc tccctccact
     1741 gccgcacctc ttcagccgcg agggctgcct tttcgccgtc gagcccgccg tccctggcga
     1801 tgtaggccgt ggtggagggg aaagcgaaac ccgaaccgga tttttccacg atctccccga
     1861 tccggaagag gatgtcctcc tggacggcca ggaattccgc ccactttttc gtcctgatgt
     1921 aagcgaagac aagaatgtcc agggacgagg ctccgtagga ctcgaaccgg gcgaagggaa
     1981 ccgggtcggc cgccactttc gggtgggcgt ggagcacatc ccggacggac gccaggatcc
     2041 accggagctg gtctccggtg gtctcgtacc ggaggctgag ggttttttgc atcagcgaac
     2101 cgtttctccg ggtgacgttc acgatctgca tctgggagaa ctccgcgttg gggatggaga
     2161 tcaccgtccg gtcgagggcc tcgatcttcg tggagcggag tccgatgtgg aggaccgtgc
     2221 ccgccgtgtt gccgaaccgg cagtattctc cgaccttcac ggaccggtcg gcgaaaaggg
     2281 tgagtcctcc gatgatgttc tccaccgtgg gcttggcggc cagggagatg gccagtccca
     2341 ccacgcccag tccggtgagg agatggaaga cggacagccc cagccgggag agaccgtaga
     2401 gaaggatcca cgaagccgcg gtgatgcaga tgagcctgct cacggtccgg acgaggctgg
     2461 cgtcgatgct ctccggttcc accctgggcg acaggatgat cagctcggcg atagcctctc
     2521 ccacccggat ggcgcaccac gcccagccga accagcggac tgcggagaag aactggatga
     2581 gaaagatgca cagcctgccg gtgatgttga tggtgtcgtc cacgagcaga attgtctggg
     2641 tcgaggtcag ggagagtgcc accgggggaa ggatccggag gatattcccc gcgaagaggc
     2701 tcatgttccg ggagattttt ccgaacagtc tc
//
LOCUS       DAOTHD010000204         2729 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_44080_length_2729_cov_3.179132, whole genome shotgun sequence.
ACCESSION   DAOTHD010000204 DAOTHD010000000
VERSION     DAOTHD010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44080_length_2729_cov_3.179132"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1449)
                     /locus_tag="P5219_04550"
     CDS             complement(52..1449)
                     /locus_tag="P5219_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRX26055.1"
                     /translation="MFRKTMVLTLAASVLFSSLPRAAVSAELYAEEEAVLFEAGADYP
                     VAPAGKRGQRVVVRALVRPSGKVRERAPLAVALVLDKSGSMASDGKMENAKKGALEAL
                     KMLDSRDVAAVVVYDDRASVLVRARTVGRGEDDPAFYRAIQRLRPGGSTALYHGTVTG
                     AAELQPFIDEGYIPRIVLLSDGIANVGPSSTEDLAALGRNLSRQDITITTIGLGLDYN
                     EDLMTALAAESGGNAYFARHGGMLPEIFARDLEDAVTVTARKVRVTLRCPEGVRPVRV
                     IGRQGKVEGSSLEVAIDNLYGGEKYALFEVELPEGQDGTVLKAAELEVEYADAASGTV
                     VRKNAELAVSFSGDAGAVEKNRRSDILSQAELARNAEIREAAVRLADEGKAREAAQLL
                     DERTKQLQAAAPAAGSAAPKLEQEAGYFEELAESLLEAGVFSSSQRKAVLNDAYTQKN
                     QQAPVSGDVEDEEEK"
     gene            complement(1522..2298)
                     /locus_tag="P5219_04555"
     CDS             complement(1522..2298)
                     /locus_tag="P5219_04555"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HRX26056.1"
                     /translation="MDDTTAREELRFFLKDTVRRSVDFSRTAQNLGAPPPPVQKPVPG
                     DAARISLPGPEKWTAVGDLSLREAMERRRSVRQFSNRPFSLEELAFLLWATQGVRGES
                     DPVRTYRTVPSAGCRHPFETYLAVFRVEGLEKGLYRYLPLEHALLPLGHPDRLEEETA
                     RAALGQKFAGKGAVTFFWTALPWRTEWRYSEASVKVIALDGGHICQNLYLACEAIGAG
                     TCAIAAYDQDAADRLVGADGKDEFVVYMAPAGKKGDFTTP"
BASE COUNT          488 a          926 c          790 g          525 t
ORIGIN      
        1 tcccctccgg gcggcgggag agaaagtcct ctcccccgcc ggatactctt tttacttttc
       61 ctcttcgtct tccacgtcac cgctcacggg agcctgctgg ttcttctgcg tgtaggcgtc
      121 gttgagaact gccttcctct ggctgctgga aaaaaccccc gcttcgagca ggctttccgc
      181 cagttcttcg aaatagcccg cctcctgctc gagtttcggg gcggcggaac cggcagccgg
      241 agccgcggcc tggagctgtt tcgtccgttc atcgagaagc tgtgccgcct ccctggcttt
      301 cccctcgtcg gccagccgga cggccgcttc ccgtatctcg gcgttcctgg ccagttcggc
      361 ctgggagagg atgtcagacc gccggttctt ctccaccgcc ccggcatctc ccgagaagga
      421 gaccgcaagc tccgcgttct tcctgaccac ggtccccgac gcggcgtcgg cgtactccac
      481 ttccagctcc gcggccttca gaacagtccc gtcctgtccc tcgggaagct ccacctcgaa
      541 aagggcatac ttttctcctc cgtacaggtt gtcgatggca acttcaagag agcttccctc
      601 caccttcccc tgccgcccga tgacccgcac cggccggacg ccctcgggac accggagggt
      661 caccctcact ttccgggccg tcaccgtaac ggcatcctcc agatccctgg cgaatatctc
      721 gggaagcatc ccgccgtgcc tggcaaaata ggcgtttccg ccgctttcgg cagccagggc
      781 ggtcatcagg tcttcattat agtccaggcc gaggccgatg gtggtgatgg tgatatcctg
      841 gcgggacagg ttccttccca gggccgccag gtcctccgtc gaggagggcc ccacgttggc
      901 gatgccgtcg gagagcagca cgatcctggg gatgtatccc tcgtcgatga agggctgcag
      961 ttcggcggct cccgtcaccg ttccgtgata gagcgccgtg gaccctcccg gccgaaggcg
     1021 ctgtatcgcc ctgtagaaag cggggtcgtc ctccccgcgg cccacggtcc gcgcccggac
     1081 gagcacggaa gcccggtcgt cataaaccac aacggcggcc acgtcccggg agtccagcat
     1141 cttcagggct tcaagggctc ccttcttcgc gttctccatc ttgccgtccg acgccatgga
     1201 ccccgattta tccagcacca gggcaacggc gaggggggca cgctcgcgca cctttcctga
     1261 aggccgcacc agcgcccgga cgacaacccg ctggcccctt tttcccgccg gggcgaccgg
     1321 ataatcggcc cccgcctcga agaggacggc ctcttcttcg gcgtaaagct ccgctgaaac
     1381 cgccgcccgg ggcaaagagg aaaaaaggac cgatgcagcc agggtgagca ccattgtttt
     1441 tctgaacatg atcaaccacc tcctggacgg aaggatagcc cgggaagggg gcggatgtgt
     1501 cagggcggtg tcaccttcat gtcacggagt tgtaaaatct cccttctttc ccgccggggc
     1561 catgtagacc acgaattcgt ccttcccgtc cgccccgacc agcctgtcgg cggcatcctg
     1621 gtcataggcc gcgatggcgc aggtccccgc gccgatggcc tcgcaggcga ggtagaggtt
     1681 ctggcagata tgaccgccgt ccagggcgat gacctttacg gaagcctccg aatagcgcca
     1741 ttccgtcctc cagggaaggg ccgtccagaa aaaggtcacc gctcccttcc cggcgaattt
     1801 ctgaccgagg gccgcccgcg ccgtctcctc ctcgagacgg tccggatgcc ccaggggcag
     1861 aagggcatgc tccaggggaa ggtaccgata gagtcccttc tccaggccct cgacccggaa
     1921 gaccgccagg tacgtctcga agggatgcct gcaccccgcc gacgggacgg tgcggtacgt
     1981 ccgcaccggg tcggattcgc cccggactcc ctgggttgcc cagagcagga aggccagttc
     2041 ttcgagtgaa aagggccggt tggagaactg cctcacggac cgacggcgct ccatggcctc
     2101 ccgaagggac agatcaccca cggcggtcca cttctccggt ccgggcaggg aaatccgggc
     2161 cgcatccccg gggaccggct tctgcacggg aggcggcgga gcccccagat tctgggcagt
     2221 cctggaaaaa tccacggacc ggcgcaccgt atccttcagg aagaagcgga gctcttccct
     2281 tgctgttgtg tcgtccacga aaaaacctcc ttcggcattt ctgtttttct gattgtagca
     2341 accgttcctt cccctctcaa gagccggaga aaaagcgttc tgttgcaaaa acccttctct
     2401 tttgctagag tttcccggag aaagttatca caactttccg aattcacttg atccaacgga
     2461 agggagggag gttcttcggg tacaaggcaa gagcagaaag acctgagagg tgttgtgctg
     2521 tacgagtctt cgcaatacgg tgtgaaaagg aggctgaatg caatggcagg cttcaccaga
     2581 aaaacggtcc tggttctgct ggctgcggct cttgttcttc tccccgcgtc cgctcccgcc
     2641 gccgaaccat ccctcgccga gatctactcc atcctgaaga cgaaggagtt cgtggacctc
     2701 acccacacct tcgacaccga aatccccca
//
LOCUS       DAOTHD010000205         2725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_44185_length_2725_cov_3.217603, whole genome shotgun sequence.
ACCESSION   DAOTHD010000205 DAOTHD010000000
VERSION     DAOTHD010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44185_length_2725_cov_3.217603"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /locus_tag="P5219_04560"
     CDS             <1..1311
                     /locus_tag="P5219_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRX26057.1"
                     /translation="RAGDHRRRARDPDSSFGPDGKPVGIYFSGIPMNVLEETRNSVVR
                     TVALLAAAVLVVGLLFQGWLVRKIVKGVRSMVHYMKEAEEGRLCAAREDFDVRSRDEV
                     GDMADALASMTSKLCAIISELREDALVSAQKAQSLAALSEETLASMEEVGGEVDRVTV
                     LSEGSAAALEETGAGVHEVAESASSAARASEEGAEAAAATRETSEKAVKEVEHSIRLI
                     RDGGQKVQETAREMQEVAKAVDSISGFVATITSIADQTNLLALNAAIEAARAGEHGRG
                     FAVVADEVRKLAEQSGHAAQEVQKLISALENGAERSLSVTGEAKSIMENTVKSAEGAL
                     QELNRAMAQIAKSTDAMQGIAAVAQEQAASAEEMTAGVEQVSRSTGEVMEAIRHIKTA
                     SGETVDASRNLAVEAQALAENAEKIDRLLSGFVVSKEAGLLPAK"
     gene            complement(1373..1981)
                     /locus_tag="P5219_04565"
     CDS             complement(1373..1981)
                     /locus_tag="P5219_04565"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRX26058.1"
                     /translation="MEWTAFLTYVLIASHTPGPNIIFAMNTSRIHGFAGALPLVSGMI
                     SGLVIIMTGCAAFNLLLVEFLPAVLPWFRAAGACYILWLAWKIAFPTLRKVRGEEVLP
                     EAPGFRQGFVLQFLNPKVILFGLTALSAFVIPWTSSKAWLFGSGLFLALACSSGVTLW
                     TAFGAAQKRIMSRQGRTVDVVMGALLAWCAFSVSGLGDILLK"
     gene            complement(2005..>2725)
                     /locus_tag="P5219_04570"
     CDS             complement(2005..>2725)
                     /locus_tag="P5219_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26059.1"
                     /translation="ILAETGNEFFRRKLRPLLEEFRNAGGSLASLRRRLDERLAAEAP
                     QLRKSLVKNFPTVLRGLEEETRELTARWFAAHGVRAPRRILRLEQESGKAGGAFSFQT
                     PGMEGVVNVLAGVVGSLVTAGTALLCGGTGTALVAAGPVGLAVGGAAGLVLSALLVRC
                     GRDRVKKMAEDIPLPPRVAALLVTEAGMEEAERNLEKAMEEWLDSRLAEAGIELEDHV
                     RALVADEMKSLTEIGGLRPVS"
BASE COUNT          516 a          851 c          914 g          444 t
ORIGIN      
        1 agggcaggtg accaccgtcg tcgagcccgg gacccagatt cctctttcgg cccggacgga
       61 aagcccgtag gcatttattt ctccggcatt cccatgaacg tcctcgaaga gacccggaat
      121 tccgtggtcc ggacggtggc tctcctggcc gcggcggtcc ttgtggtggg gctgctcttc
      181 caggggtggc tcgtgcggaa gatcgtgaag ggtgtccgct ccatggtcca ttacatgaag
      241 gaggccgagg agggccgcct gtgcgccgcc cgggaggact tcgatgtccg ttcccgggat
      301 gaggtgggcg acatggccga tgccctcgct tccatgacct cgaagctctg cgccatcatc
      361 agcgagctcc gggaggatgc cctggtgtcc gcccagaagg cccagtccct ggccgcactc
      421 tcagaggaga ctcttgcctc catggaggaa gtgggcggcg aggttgacag ggtgacggtc
      481 ctctcggaag gcagcgccgc tgctcttgag gagacgggcg caggtgttca cgaggttgcg
      541 gagagcgcct catcggcggc ccgggcttcc gaggaagggg cggaggccgc tgccgcgacg
      601 agggagacca gcgaaaaggc cgtgaaggaa gtggaacact ccatacgcct gatccgggac
      661 ggtgggcaga aagtccagga gacggcaagg gaaatgcagg aagtggcgaa ggcagtggac
      721 tccatttccg gatttgtggc caccatcacc tccatcgccg accagacgaa ccttctcgcc
      781 ctcaacgccg ctatagaggc ggcccgggcc ggggaacacg gccggggatt cgccgtcgtc
      841 gccgacgagg tgcggaagct ggcggagcag tccggccacg cggcccagga agtccagaag
      901 ctcatttccg ccctcgagaa tggcgctgag cggtcccttt ccgtcaccgg agaagcgaag
      961 agcattatgg agaacacggt gaaatccgct gaaggggccc ttcaggagct caaccgtgcc
     1021 atggcccaga tcgccaagtc aacggacgcc atgcagggca tcgccgcagt ggctcaggag
     1081 caggcggcgt ctgccgagga gatgacagcg ggagtggagc aggtctcccg gtccaccggg
     1141 gaggtcatgg aggccatccg gcacatcaag accgcctcgg gggagactgt ggatgcgtcc
     1201 cgcaacctgg cggtggaagc ccaggccctg gctgagaacg ccgagaagat cgacaggctg
     1261 ctttccgggt tcgtggtatc gaaagaagcg gggcttctcc cggcgaagta ggattttccg
     1321 gcaggtgtcc aaagccgccc ccggatgctc cgggggcggc gtctttatcc ggctatttca
     1381 gaaggatgtc cccgaggccg gagacggaga aggcgcacca ggccagcagg gctcccatga
     1441 cgacgtccac ggttcgtccc tgcctggaca tgatccgctt ctgggccgcc ccgaaggcgg
     1501 tccagagggt cactccgctc gagcaggcaa gggcgaggaa gagccctgag ccgaagagcc
     1561 aggctttcga ggacgtccag gggattacga aggcggagag ggcagtcagg ccgaagagga
     1621 tcaccttcgg gttgaggaac tggaggacga agccctgccg gaatcccggc gcttcgggaa
     1681 gaacctcttc gcccctcact ttccgcagag tggggaaagc tattttccat gcaagccaga
     1741 ggatatagca cgcccccgcc gcccggaacc agggcagcac tgcgggcaga aactccacca
     1801 gcagaaggtt gaatgccgcg cagcccgtca tgatgatgac gagcccggag atcatgccgg
     1861 acaccagggg cagggcaccg gcaaagccgt ggatcctgga agtgttcatg gcgaagatga
     1921 tgttcggccc cggggtatgg gacgcaataa gtacataggt gagaaaggcg gtccattcca
     1981 tggcggtacc tccttcggtc cgggtcagga gaccggccgc agccccccga tctccgtcag
     2041 ggatttcatt tcgtccgcca ccagggcgcg gacatgatct tcaagctcga tcccggcctc
     2101 ggcaagcctg gaatcgagcc attcctccat ggccttttcg agatttctct ccgcttcctc
     2161 cattcccgct tccgtcacaa gaagagccgc aacccgggga gggaggggaa tgtcctcggc
     2221 catttttttt accctgtccc gcccgcagcg caccaggagc gccgacagga caagccctgc
     2281 cgctcccccc accgcaagcc ccactgggcc ggcggccacg agggccgtcc ccgtcccccc
     2341 gcagagcagg gcagtccctg cggttaccag gctgccgacc acgcccgcga ggacgttcac
     2401 gacgccctcc atcccggggg tctgaaagga gaaggccccg cctgcttttc cgctttcctg
     2461 ctcaaggcgg aggattcttc ggggagccct gacgccgtgg gcggcgaacc accgggccgt
     2521 gagttcccgg gtttcttcct caaggcccct gagaacggtg gggaagtttt ttaccaggct
     2581 tttccgaagc tgaggggctt cggcggcgag ccgttcatca agccgcctgc ggagggacgc
     2641 gagggatccc ccggcgttcc ggaactcttc cagcaggggg cgcagcttcc tgcgaaagaa
     2701 ttcgttcccc gtctcggcga gaatc
//
LOCUS       DAOTHD010000206         2724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_44198_length_2724_cov_3.548520, whole genome shotgun sequence.
ACCESSION   DAOTHD010000206 DAOTHD010000000
VERSION     DAOTHD010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44198_length_2724_cov_3.548520"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="P5219_04575"
     CDS             complement(<1..202)
                     /locus_tag="P5219_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26060.1"
                     /translation="MTCSKCNAGKLRDIPRADDAVQPAGIERRIVFAPGKFWDDYVAR
                     YFTVEKGAQTPFHTHDWPHYILI"
     gene            426..1397
                     /locus_tag="P5219_04580"
     CDS             426..1397
                     /locus_tag="P5219_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRX26061.1"
                     /translation="MKKIAAVLSVVFLFLVLAASSAPASDIKLAHVVNENDAFHVCAL
                     KFKELVEGGSKGELTVTIYPNAKLGDERNLLESMRMGVVDSAIITGGPIINFMPKFGV
                     FDLPFLFTTPEQAYKVLDGEIGRGMLADMDALGWKGLAYGERGFRNLTNSKKPVTVPD
                     DMKGLKIRLMQNPVYVDSFKALGANAVPMAWTEALTALQQGTIDGQENPLNVIVSFNL
                     FESQKHLALTRHAYAPNVIMMSKRTWDKLSPEHQKLVQESAQAAAEHNRAYDNEKEAE
                     WLQFLKDKGMEVTEPDLSLFREAVKPVYEKYEEQFGKELVEAILAVK"
     gene            1410..1919
                     /locus_tag="P5219_04585"
     CDS             1410..1919
                     /locus_tag="P5219_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRX26062.1"
                     /translation="MERNRSEARGETKRSPLLQLSDAVNTVSEVVLFGMILAMIAVTT
                     LQIVCRIFFDALIWSEELTTYLLVASSLLGAAVGFKRGSHIAVTFLVNKLPEAPRKAV
                     SVFVQCLGVFFFAVVAYYGADLMKSEAMQTTPAMGISMTWIYVMYPVIGGIILLHLLA
                     GFGDILGRR"
     gene            1922..>2724
                     /locus_tag="P5219_04590"
     CDS             1922..>2724
                     /locus_tag="P5219_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRX26063.1"
                     /translation="MGLVLAGSFILFMVLGVPVGLAIGAAGLLVVLLSGFPLAMIPQT
                     MFSGNESFALVAVPFFVLAGDILARGGISERIVTFAEATLGRFRGGLSIVSTVASMFI
                     AAISGSGAATTAAVGSALLPQLKKKGYDVDFSAALIAASGTIGVVIPPSVPMVLYAVI
                     AGISVAKLFMAGFVPGFLMGIGLIVYSMYVARKRGYPAAEATTGRQKWKLFLEASWGL
                     LTPVIILGGIFSGFFTPSEAAAVAVDYSLIVAFFIYKSMDFKKFYNLVVG"
BASE COUNT          571 a          784 c          761 g          608 t
ORIGIN      
        1 tgatcaggat gtaatgaggc cagtcgtggg tgtggaaggg ggtctgagct cccttttcca
       61 cggtgaaata acgggcgaca taatcgtccc agaattttcc cggggcgaaa acgatcctcc
      121 gctcgattcc ggccggctgg acggcatcgt cggccctggg gatatctctc agctttccgg
      181 cgttgcattt ggaacacgtc atgaaattca cgctccctgg gtacattgga tttcttccct
      241 tctcccggtc gggaaaggaa gcaagcagaa tggtaccatc ggggggtgtc cctgtccaga
      301 acgacaggcg gcagaggagc cgggagaaac aaaaacttgc aattctcacc ccattgtcta
      361 taatgcttta tcacaatgga acgtcagttt catatattct gcatcaggaa tgggaggaaa
      421 gaagcatgaa gaaaatcgca gcggttttat cggtagtttt tcttttcctg gttcttgcgg
      481 catcgtccgc accggcctcc gacatcaagc tggcccacgt ggtgaacgaa aacgacgcct
      541 tccacgtatg tgccctcaaa ttcaaggaac tcgtggaggg cgggagcaag ggtgaactga
      601 cggtcaccat ctaccccaac gcgaagctcg gcgacgaaag gaaccttctc gagagcatgc
      661 gcatgggcgt ggtggactcg gcgatcatca ccggcggccc tatcatcaac ttcatgccca
      721 agttcggcgt gttcgacctt cccttccttt tcacaacccc ggagcaggcc tacaaagttc
      781 tggacgggga aatcggcagg ggcatgcttg ccgacatgga cgccctcggc tggaagggtc
      841 tcgcatacgg cgagagggga ttccgcaacc tgacgaacag caaaaaaccc gtaactgtcc
      901 ctgacgacat gaaggggctg aagattcggc tcatgcagaa ccccgtctat gtggactcct
      961 tcaaggccct cggggcgaat gccgttccca tggcctggac cgaagccctc accgccctcc
     1021 agcaggggac catcgacggc caggagaacc ccctcaacgt catcgtatcc ttcaaccttt
     1081 tcgagtccca gaagcacctt gccctgacgc ggcatgccta tgcccccaac gtgatcatga
     1141 tgagcaagag aacatgggac aagctttctc ccgagcacca gaagctcgtc caggaatccg
     1201 cccaggctgc agcggagcat aacagggcct atgacaacga aaaggaagcc gagtggctcc
     1261 agttcctgaa ggacaagggc atggaagtca ccgaacctga cctctccctg ttcagggaag
     1321 cggtcaagcc cgtgtacgaa aaatacgaag agcagttcgg caaagagctt gtcgaggcta
     1381 tccttgccgt gaagtaggca ccggcaggaa tggaacggaa taggagcgaa gcaagaggcg
     1441 agacgaagcg ctcgcctctt ctccaattaa gcgatgcagt gaatacggtc agcgaggtcg
     1501 tccttttcgg aatgattctt gccatgattg ccgtgactac cctccagata gtctgccgca
     1561 tctttttcga tgcccttatc tggtcggaag agctcaccac ctaccttctc gtcgcgtcat
     1621 cccttctggg cgccgcggtg ggattcaaaa gaggatccca catcgccgtt acctttctcg
     1681 tgaataagct ccctgaagct cccagaaaag ccgtctctgt atttgtccag tgcctcggcg
     1741 tgtttttctt tgccgtggtg gcctattacg gggcggatct catgaaaagc gaggccatgc
     1801 agaccacgcc ggctatggga atatcaatga cgtggattta cgtcatgtac cccgtgatcg
     1861 ggggcatcat tctgcttcat cttctcgccg ggttcggcga catcctgggg aggcgctgac
     1921 catgggactc gttcttgcag gcagctttat cctcttcatg gtactgggcg ttccggtggg
     1981 gctcgccatc ggggcggcag gactgctcgt cgtgctgctg agcggctttc cccttgccat
     2041 gattccccag accatgtttt cgggcaacga gtcttttgcc ctcgtggccg ttcccttttt
     2101 cgtccttgca ggagacattc ttgccagggg gggcatctcc gaacggatcg tcacctttgc
     2161 cgaagccacc ctcggcagat tccggggagg gctttccatc gtctccactg tggcatccat
     2221 gttcatagcg gccatctcgg gatccggggc ggcgacgacg gcagccgtgg gctccgctct
     2281 tctcccccag ctcaagaaaa aggggtacga cgtggacttc tccgcggcac ttatagccgc
     2341 gtccggaacc atcggagtgg tcatcccccc cagcgttccc atggtgcttt atgccgtcat
     2401 cgcgggcata tctgtggcca agctcttcat ggcggggttc gtgcccggat ttctcatggg
     2461 aatcggtctt atcgtgtact ccatgtatgt ggcgaggaag aggggatatc cagcagccga
     2521 agccaccacg ggaaggcaga aatggaaact ttttctcgag gcgtcctggg ggcttctcac
     2581 tcccgtgatc atcctgggag gtatcttctc gggctttttc accccgtcgg aagccgccgc
     2641 cgtcgcggtg gactattctc tcatcgtggc ctttttcatc tacaaatcca tggacttcaa
     2701 aaaattctac aatctcgtgg tggg
//
LOCUS       DAOTHD010000207         2716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_44359_length_2716_cov_3.190530, whole genome shotgun sequence.
ACCESSION   DAOTHD010000207 DAOTHD010000000
VERSION     DAOTHD010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44359_length_2716_cov_3.190530"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..402
                     /locus_tag="P5219_04595"
     CDS             214..402
                     /locus_tag="P5219_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26064.1"
                     /translation="MFGKILSTGFLIGGYVFLGVYIARRLVASGYPNWLNAALPAVLA
                     LFGLAQGWFLVREIMRKK"
     gene            453..935
                     /locus_tag="P5219_04600"
     CDS             453..935
                     /locus_tag="P5219_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HRX26065.1"
                     /translation="MENIFAPWRMAYIQVSKKQDGCIFCDFPPEHRDEERLILHRGKT
                     CFVIFNAFPYNPGHLMVAPYRHTAEYELLTDDEMLEMHRLGGKCISVLKKVMNPQGFN
                     LGINLGKVAGAGFDGHLHLHIVPRWNGDTNFMPVFADVRVVAEELAATYGRMKEAWNE
                     "
     gene            932..1567
                     /locus_tag="P5219_04605"
     CDS             932..1567
                     /locus_tag="P5219_04605"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HRX26066.1"
                     /translation="MSDLFIEPARETVTEAKIQKSRFIAHVCISTDETRARERIKIIS
                     SEHRQANHNCWAYRVGTLKREEYFSDDGEPAGTAGKPILGAILRQEMTNVLVVVTRYF
                     GGIKLGVRGLIEAYGNTASEGLLASGKISRRIRTTYEVRMPYDMAKLMDRLLDSCDTG
                     VEFRTSGYGADVTVQCAVPLDCVQKVESTLDEWKNARRIIDWERNSGTPGV"
     gene            2178..>2716
                     /locus_tag="P5219_04610"
     CDS             2178..>2716
                     /locus_tag="P5219_04610"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HRX26067.1"
                     /translation="MKIRFQTLILAAFFTGLTAVGAFVRIPLPIVPITLQVLMVLLSG
                     LLLPPGGALFSQSAYILLGLAGIPIFAGGGGISYIIAPTFGYLLGQIPASWAISMLVQ
                     RGERSFFSMFLASIAGIGIIYTLGAVLLFLNLTLLAGKSIGFVQILKIGVYPFILPDL
                     LKAGVASILALKIRRVLHTR"
BASE COUNT          722 a          704 c          672 g          618 t
ORIGIN      
        1 gagagatgac tgcaccggaa ccggtgcagt catctctcag ggggcttgga atctctcttc
       61 gggtgacggg aaacccgaac cgcatgaaag tgtctctcga cgggctgaac aaaagccaga
      121 tggaggcctt tgttcgcctt ctcgaagaac ggggggaaga actatttccc gggaaatagg
      181 tgataaaagg tgaacaaatt caaggatgta gtaatgtttg gaaaaattct atcgacggga
      241 ttccttatcg gcggatatgt gtttcttggc gtctatattg cccgaagact tgtagcctcg
      301 gggtatccca actggctcaa tgcagccctt cctgccgttc tcgccctctt cggcctcgcc
      361 caggggtggt ttcttgtccg ggaaataatg agaaaaaaat agcaaacagg tttcacgtga
      421 aacagaacga agaacaggaa ggatgatgga ccatggaaaa cattttcgcc ccctggagaa
      481 tggcctatat acaggtatca aaaaagcagg acggctgcat cttctgcgat ttcccgccgg
      541 aacaccgtga cgaggaacgg ctcattcttc acaggggaaa aacatgcttc gttattttca
      601 acgcctttcc gtataatccc ggccatctca tggtggctcc ctaccgacat acagccgaat
      661 acgaactcct taccgacgat gagatgctcg agatgcaccg gctgggagga aagtgcattt
      721 cggtgctgaa aaaagtgatg aacccccagg gcttcaacct cggcatcaac ctcggaaagg
      781 tagccggcgc aggcttcgat ggccacctcc acctccacat cgttccgaga tggaacggag
      841 acaccaattt catgcccgtg ttcgctgatg ttcgtgtcgt cgccgaggag ctggcggcaa
      901 cctatggacg gatgaaggaa gcctggaatg aatgagtgac ctgttcatcg agcctgcccg
      961 cgaaaccgtc acggaagcga aaatccagaa atcccgcttc atagcccacg tatgcatcag
     1021 caccgatgaa acccgggctc gtgaaagaat taaaatcatc tcatcggagc accgccaggc
     1081 aaaccacaat tgctgggcgt accgggtagg taccctgaaa agagaggaat acttctccga
     1141 cgacggcgaa ccggccggaa cggcgggaaa acccattctc ggagctattc tccggcagga
     1201 aatgacgaat gtcctggtgg tggtgacccg gtatttcggc ggcatcaagc ttggtgtacg
     1261 gggcctcata gaagcctatg gaaacacagc ctcggaagga cttcttgcct ccgggaaaat
     1321 ttcccgaagg atacggacga cctatgaagt ccggatgccc tatgacatgg ccaagctgat
     1381 ggaccgtctg ctggattcct gcgatacggg ggtggaattt cgcacctccg ggtacggtgc
     1441 tgacgtgacg gtacagtgcg ccgtccctct cgactgtgtt caaaaggtcg aatcaaccct
     1501 cgacgagtgg aagaacgcac ggagaatcat tgactgggag agaaacagcg gaactccagg
     1561 agtgtaattt ccgaagccgg taatttttcc cgaatcgaag agtcccggca ggaagagtat
     1621 aaaagcctgc agcgacgcca ccttttctag ggtgacaaaa gagaagaacc ctcgtttcaa
     1681 aaagaatatc ctcctctcga tcttccgcct gacgatgaat tgaaacacta cggactgcaa
     1741 tgaacacctt ttcaacaatc aggtgaagtt ccctgcatcc cgggaagtct ccccactgaa
     1801 gaagacatgc cgtctttcag cctcgggaaa tagatacatc gacaagaatc cgctgactgc
     1861 agcggaaagg gcatatttat ttccacatct ggaaacgaaa atgatggatc ttatccgaaa
     1921 aaaaccctcc tgtttccgga gaaatactcc gatttctgct actgagcgaa caaaggtcag
     1981 acaagatcaa tgacaagata cacacatcgg atcctatata cccgaaaaat caccggaaaa
     2041 acttcatcag cccgcgagaa cggccggaaa aggaatccat tgcgggaaaa cgtacctcag
     2101 acgatctttt tccatcagac tcttgaaaaa ttctatgtca accaataaaa tataaggtag
     2161 acaggagatg atttctcgtg aaaatccgtt ttcaaaccct cattctagct gcttttttta
     2221 ccggtcttac tgcggtcggc gcatttgtac gaatacccct tccaatagta ccaatcacac
     2281 tccaggttct catggttctt ctttcgggcc tgctgcttcc accggggggc gcactctttt
     2341 cccaatcggc ctatatactc cttggacttg ccggaattcc catatttgcc ggaggaggag
     2401 gaatatcgta tatcattgcc ccgacattcg gctatttgct gggccagatt cccgcctcat
     2461 gggccatatc gatgctcgtt caaagaggtg aaagatcatt tttctcgatg tttctggcgt
     2521 caatcgccgg cattgggatc atatatacac tcggtgcggt tctccttttt ctgaatctca
     2581 ctctccttgc aggaaaatca atcggctttg ttcagattct aaaaataggg gtctatccct
     2641 tcattctccc ggacctcctg aaagccggtg tggcttcaat actggccctg aaaatcagaa
     2701 gagtactgca cacccg
//
LOCUS       DAOTHD010000208         2693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_44857_length_2693_cov_3.166035, whole genome shotgun sequence.
ACCESSION   DAOTHD010000208 DAOTHD010000000
VERSION     DAOTHD010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44857_length_2693_cov_3.166035"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /locus_tag="P5219_04615"
     CDS             <1..1172
                     /locus_tag="P5219_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRX26068.1"
                     /translation="LGVAVRNDTAALRVLTRGNSVCGLLAWNGRSGEVLHFRTGIVIL
                     AAGGGAGLYSRNNNTADVSCDSFGLAYEAGAVLRDMEMVQFYPSMMYEPVRIPISNPL
                     FGEGAVLRNALGEEFLCRYSPMGNHATRDLMARAVKQEIDEGRGNPGYVYVDCSGIPG
                     NVLEMRYGELSAILARYGLNPEKDLLPVAPSAHFYLGGVDIDRTCATSVEGLLACGEA
                     AGGLHGANRLAGIALAEAAVFGKIAGRTASTLFKKHQPPASPAEPDVVLPGSPGVPLA
                     ELTEKLREMLWDCASLVRSGASLEKAEREIGEISRELEGGGRGNAEDPPRYFALRSMV
                     LTARMLVKCSLLRKETRGAFCREEHPESSGRFAGSFIVQKTEAGAMAVRFRPNWS"
     gene            complement(1189..1989)
                     /locus_tag="P5219_04620"
     CDS             complement(1189..1989)
                     /locus_tag="P5219_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRX26069.1"
                     /translation="MKGNTKEDGASALKKGLSVLSCFSWQKSRRTLTEIARELNLPLP
                     TAARLAKALEGQGFLEREKKSRLYSLGFQCYLLGAIAKKTGGLRTLALPCMEELRKRF
                     NETVNLYVREGDFRICYEQVESSLNLKRSAKLGDRFPLWAGASGRCFLAFMPEEEVSR
                     ILDEAQSLTPNTILDKKVVTEKNSELRKRGYSFSLSEREQGVSSVAVPIFDASLQPVA
                     CMTLSGPTARFTEEMIHELIPALKKTCLDLSLKLGAERRSVSLLETDR"
     gene            2126..>2693
                     /locus_tag="P5219_04625"
     CDS             2126..>2693
                     /locus_tag="P5219_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HRX26070.1"
                     /translation="MKKSAVLRQMLQERKAVPVPGAHDAISAKLIEKTGFKAIQVSGF
                     GLAASLLGLPDMAFISFTEVLEFTRNIVDAVNIPVMADADTGYGNAINAMRVTEKFIR
                     AGAAGMNIEDQVFPKRCGHIEGKTVVPLEEMVLKVRACAKVRDQLDPDFIINARTDAI
                     AVSGVDEAIRRGNAYIEAGADMIFVEAPR"
BASE COUNT          565 a          767 c          786 g          575 t
ORIGIN      
        1 aactcggtgt cgccgtcagg aacgataccg cggccctcag ggtccttacc cgcggaaatt
       61 ccgtctgcgg tctcctggca tggaacggcc gctcgggaga ggttcttcac ttcaggacgg
      121 gaatagtcat cctggccgcc ggcgggggag cgggtctcta ttccaggaac aacaacacgg
      181 cggatgtgtc ctgcgattcc ttcggactgg cctacgaagc gggggcggtg ctccgggaca
      241 tggaaatggt gcagttctac ccctccatga tgtacgagcc ggtcagaatc cccatttcca
      301 atcccctctt cggcgaaggt gccgttctca ggaatgcact gggggaggag tttctctgcc
      361 ggtacagccc aatgggcaat catgccacca gggatctcat ggcaagggcg gtgaaacagg
      421 aaatcgacga agggaggggc aatccggggt acgtgtacgt ggactgctcc ggtattcccg
      481 gaaatgtgct ggagatgagg tacggcgaac tttcggcaat tctcgcacga tacggcctga
      541 acccggagaa ggaccttctt cctgtcgctc cctcggccca cttttacctc ggaggggtgg
      601 acatcgacag gacctgcgcc acttccgtcg aaggcctgct cgcctgcggc gaggcagcgg
      661 gagggcttca cggggcgaac cgcctcgcgg gaattgccct ggccgaggcg gcagtgttcg
      721 ggaaaatcgc cggcagaacg gcgtccaccc ttttcaaaaa acatcagcct cctgcttcgc
      781 cggcggagcc ggacgttgtg cttcccggaa gtcccggtgt tcctcttgcg gagctgacgg
      841 agaagctgcg ggaaatgctg tgggactgcg cttccctcgt ccgcagcggg gcctccctgg
      901 aaaaggctga gcgggagatc ggagaaatct cccgggagct cgaaggggga ggaaggggga
      961 acgcagaaga tcctccccgg tattttgccc tccggtccat ggtgctgacg gcgcggatgc
     1021 ttgtaaagtg cagcctcctc cggaaagaaa cccggggcgc cttctgccgg gaggagcacc
     1081 cggagagttc cggtcgtttc gcgggctcgt tcattgttca aaagacggaa gccggggcca
     1141 tggcggtccg cttccggccg aactggtcat gaactgaaag gtttttggtc aacggtccgt
     1201 ttcaagaaga gaaacggacc ggcgttcggc tccgagtttc agtgaaaggt ccaggcatgt
     1261 cttcttcagt gcgggaatga gttcatgtat catctcttcc gtgaagcggg ccgtcggacc
     1321 tgagagggtc atgcacgcca caggctgcag agaggcgtca aagatgggca cggccacgga
     1381 agagaccccc tgttcccgtt ccgaaaggct gaagctgtat ccacgctttc gcagctcact
     1441 gtttttttcc gtgaccactt ttttgtcaag aatcgtattc ggtgtcaggg attgggcttc
     1501 gtccaaaatc cggcttacct cctcttccgg catgaatgcg agaaaacacc ggcccgatgc
     1561 gcctgcccac aggggaaaac ggtcgccaag tttcgccgaa cgcttcaggt tgagagaact
     1621 ctcgacctgt tcgtagcata ttcgaaagtc accttcccgg acgtaaagat tcactgtttc
     1681 gttaaatctt tttctgagct cttccataca gggaagagca agagtcctca ggccccccgt
     1741 ttttttcgct atcgccccaa ggagatagca ctgaaaacca aggctgtaga gtctgctctt
     1801 tttttcccgc tccagaaagc cctgcccctc cagtgctttt gccagccgtg cggccgtggg
     1861 caggggaagg ttcagctccc gggcgatttc cgtcagggtt ctcctgcttt tctgccagga
     1921 aaaacaggaa aggaccgaca gtcctttttt cagggccgac gccccgtcct ctttcgtgtt
     1981 ccccttcatg atccttttca gcctccagca ggaaaatatt ttgcattgac aaaacaggtt
     2041 caccgtgtaa tatgatttta ccacaatatg aaatgtactt ccaatatatg gaagtatttc
     2101 tatccccatt tcacggaggc agattatgaa aaaatccgca gtgcttcgcc agatgcttca
     2161 ggaacgaaaa gcagttccgg tgcccggcgc ccatgacgcc atttccgcaa aactcatcga
     2221 aaagacaggc ttcaaggcaa tccaggtcag cggtttcggc ctcgccgcgt ccctcctcgg
     2281 gcttcccgac atggcgttta tttccttcac tgaagtgctc gaattcacca ggaatatcgt
     2341 ggatgcagtc aatatccccg tcatggccga cgctgacacg ggctacggaa acgccatcaa
     2401 cgccatgcgg gtcaccgaaa agttcattcg ggcaggcgcc gcgggaatga acatcgagga
     2461 ccaggtgttc ccgaaacggt gtggtcatat cgaggggaaa accgtcgtcc ccctggagga
     2521 gatggtcctc aaggtaaggg cgtgcgcgaa agtccgggat cagcttgacc ctgatttcat
     2581 catcaatgcc aggactgacg ccatcgccgt ttccggtgtc gacgaggcca tccgcagggg
     2641 aaacgcctat atcgaagccg gggcggacat gatcttcgtg gaggctcccc gca
//
LOCUS       DAOTHD010000209         2684 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_45053_length_2684_cov_3.888931, whole genome shotgun sequence.
ACCESSION   DAOTHD010000209 DAOTHD010000000
VERSION     DAOTHD010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45053_length_2684_cov_3.888931"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="P5219_04630"
     CDS             complement(<1..199)
                     /locus_tag="P5219_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HRX26071.1"
                     /translation="MKRAVLIALGDELLSGIRNEGNCSWLAGRLTRAGWRVVCMEILP
                     DEKDSLSKYLENWVGTADLLVL"
     gene            complement(218..649)
                     /locus_tag="P5219_04635"
     CDS             complement(218..649)
                     /locus_tag="P5219_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HRX26072.1"
                     /translation="MATLCGLGFFTAMPGTVGSVAAFLVFAFYPVPLAGILAVVILGV
                     WASHRYSLKEGKTDPAEVIIDEVAGTWISMYGLPSGFFLPALFLFRIFDILKPFPVNA
                     SEKLPGGWGIMADDIVAGILVNSILSVIRWLYFGGGWSFLF"
     gene            complement(666..1958)
                     /gene="rimO"
                     /locus_tag="P5219_04640"
     CDS             complement(666..1958)
                     /gene="rimO"
                     /locus_tag="P5219_04640"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRX26073.1"
                     /translation="MKLCIVSLGCSKNTVDSENLIGLLEASGVEIVSDVGDADVALVN
                     TCGFIQPAVEESVSAILDLELLKEQGKLKKIGVVGCLVNRYGNELKKELPSVDVWARA
                     EEWGLAVRSLGLIPAAGPARGMLPETKPWTRYLKVGEGCDTFCSYCTIPSIRGRARSI
                     GIPELSSMALDLAEKGAREICLVGQDLTIYGRDLYGEPRLRDLLSELDRQLPRDVWIR
                     LLYLHPSRIDEPFIDFVAGHRRILSYLDIPVQHVDPEVLRRMNRPDDEKHIRRIFSYA
                     RKADPFFALRTTLMVGFPGETESQFSKVLDFLEQAMIDRVGAFIFSPEEGTPAADLPG
                     RVPYEIGEDRYRRLMELQSEISLERQNLFVGKKLSVLIDEIDEEDCTAWGRSFREAPD
                     VDGLIAVSGGSALKEGQLLEVRVVDAAEHDLFAEIAGE"
     gene            complement(1955..>2684)
                     /locus_tag="P5219_04645"
     CDS             complement(1955..>2684)
                     /locus_tag="P5219_04645"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HRX26074.1"
                     /translation="YLPVLSEGTEKVREEEPSVVGSCTPPTKGFKPASRFWIFAILLL
                     VAAGSSWYVWYTWDQNGVPTFAVREENSEEDKTSANKPDSGAAVEESVSGKPAQTVAG
                     EASEAGSDRQNGSVPLSADQPAAPLAGMASPPTAAELVGTNTPQSPPAAPEKKKDREL
                     VITADGDCWVRVRQGTKTLYERTLKAGESVSFTVKERIEVTYGRAGSVKTRWNGENMG
                     NPGTTRGVERVFYSPDGKTGRIAR"
BASE COUNT          611 a          792 c          693 g          588 t
ORIGIN      
        1 agaggaccag caggtccgcg gttcccaccc agttttccag gtactttgaa agagaatctt
       61 tttcgtcggg aaggatttcc atacagacaa ccctccagcc cgcccgggtc agccgtccgg
      121 caagccagga acagttgcct tcattgcgaa tgccgctgag aagttcgtct cccagggcga
      181 tcagcactgc acgcttcaag caaacgcctc cctgccgtca gaaaaggaaa ctccatcctc
      241 cgccgaagta cagccaccga atgactgaga ggatactgtt caccagaatt ccggcgacaa
      301 tatcatctgc catgattccc catcctcccg gcagtttttc cgacgcgttg accggaaaag
      361 gcttcaggat gtcaaaaatc ctgaagagga aaagcgccgg aaggaaaaac cccgaaggaa
      421 ggccgtacat ggaaatccag gttccggcga cttcatctat tatgacttct gccggatccg
      481 tttttccttc tttgagggaa taccggtggg aggcccatac tccaagaata accacggcca
      541 ggatacccgc cagggggaca ggataaaagg caaacaccaa aaaggcagcg accgatccca
      601 ccgttccggg catggcagtg aaaaaaccga gaccgcagag tgtggccata caccagaaaa
      661 aatccttatt ccccggcaat ttccgcaaaa aggtcatgtt cagcggcatc gacaacccgc
      721 acctccagta attgtccttc cttaagggct gatccgccgg atacggcgat cagcccgtcg
      781 acgtccggag cttcccggaa ggatctgccc caggcagtac aatcttcttc gtcaatctcg
      841 tcgatcagga ccgagagttt tttgcccacg aaaagattct gcctctccag ggagatttcc
      901 gactgaagtt ccatgagcct cctgtaccgg tcttccccga tttcgtaagg gacccggccc
      961 gggagatcag cggccggggt cccttcctcg ggcgagaaaa taaaggcgcc aacacgatcg
     1021 atcattgcct gttccagaaa atcgagcact tttgaaaact gggattccgt ttcgcccgga
     1081 aatccgacca tgagcgttgt cctgagagcg aagaagggat ccgccttccg ggcataggag
     1141 aaaatacgcc ggatatgttt ttcatcgtcg gggcggttca tgcgtctcag cacttcaggg
     1201 tcgacatgct ggacggggat gtcaaggtag gagagaatcc ggcggtgtcc tgcgacgaaa
     1261 tcgataaaag gctcgtcgat tctcgacggg tgcagataca ggaggcggat ccaaacgtct
     1321 ctgggaagct gcctgtccag ttcggaaagc agatccctga gacggggctc tccgtaaagg
     1381 tctcttccat atatcgtaag gtcctgcccc acaaggcaga tttccctggc acccttctca
     1441 gccaggtcca gggccatgga tgacagctcg ggaataccga tgctccttgc ccttcccctg
     1501 atggaaggta tagtgcagta ggagcagaaa gtgtcgcatc cttctcctac tttcaggtac
     1561 cgtgtccatg gttttgtttc ggggagcatc cctctcgccg ggccagccgc cggaatcagc
     1621 ccaagagacc ggactgcaag cccccactcc tccgcccggg cccatacatc cactgagggc
     1681 agctcctttt tcagctcgtt gccatagcgg tttacaaggc agccgacgac gccgattttt
     1741 ttaagcttcc cctgttcttt gagcagctcg agatccagta tggcgctgac gctctcctcc
     1801 acggcaggct gtataaaacc gcacgtattc acaagggcga cgtcggcatc accgacgtcg
     1861 ctgacaattt ccactcctga tgcctcaaga agaccgataa gattttcgct gtctacggta
     1921 ttcttggaac atccgaggct gacgatgcag agcttcacct ggcaatcctt cccgttttcc
     1981 cgtccggaga gtaaaacaca cgctccacgc ccctggtcgt tcccggattt cccatgtttt
     2041 caccattcca acgggttttg acggatcctg ctcttccgta cgtcacctcg atcctttcct
     2101 tcactgtgaa ggaaacggat tcacccgctt tcagagtgcg ctcatacaga gtcttcgtac
     2161 cttgtctcac gcggacccaa cagtctccgt ccgcagtgat taccagttcc ctgtcttttt
     2221 tcttttctgg tgcggcggga gggctctggg gggtatttgt gcccacgagt tcggcagcag
     2281 taggagggga agccattcct gcaagaggag ctgcaggttg atcggctgaa aggggaaccg
     2341 aaccgttctg ccggtcagaa ccggcttcag aagcttcacc ggcaacagtc tgagccggtt
     2401 tgccggaaac actctcttcc accgctgcac cgctgtcggg cttgttcgca gatgttttgt
     2461 cctcttcact gttctcttcc cgaactgcaa aggtaggaac cccgttctga tcccaggtgt
     2521 accagacata ccaggatgaa cccgcagcca caaggagaag aatggcaaaa atccagaaac
     2581 gggaggctgg tttgaaccct tttgtgggcg gcgtgcacga tccgaccaca gaaggttctt
     2641 cctccctcac tttctccgtt ccctcggaga ggacgggaag atat
//
LOCUS       DAOTHD010000210         2677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_45234_length_2677_cov_3.053776, whole genome shotgun sequence.
ACCESSION   DAOTHD010000210 DAOTHD010000000
VERSION     DAOTHD010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45234_length_2677_cov_3.053776"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="P5219_04650"
     CDS             complement(<1..883)
                     /locus_tag="P5219_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26075.1"
                     /translation="MKRSLLTVLVLLIVLFLSCAGYAAPSDRENSWWLKVNPIGKPPV
                     QPSETYRLLESRLRSVMAGDLDGMEFFRSLGRDGMAMRDVMAFKEEFIRLRGEELRKT
                     GALSPADALPGALEAWERSFGEAERSLADVRRKGVERAFAEGVRRYRDAHPDFPYLVR
                     MDAGASSRESYGELRFEGDIDISTLAAQVEDAVSLRDFISSAIRESFSLDMTALDALV
                     TAHRQATLDVYITQPAADWAEMDSLKRGRLQEIVVENGEVGYRDVTDPVERVFIFANL
                     KNNLRMRGGASDDLARLM"
     gene            complement(970..1737)
                     /locus_tag="P5219_04655"
     CDS             complement(970..1737)
                     /locus_tag="P5219_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRX26076.1"
                     /translation="MFTRRKFIVCLAALGVLFAAAGTSAEVDPVFVRITGSSVNIRDE
                     GSRSGRVIGRFSGGETAVVLREEQGADTFPWSLVISRVPLPDGPVVEGWVYGQYVEPL
                     PQGEWFGNTCGVQSLAFDAFFLATTETFGRTAEEAVRKLGKPLSRNDREIPGRPDPSY
                     KVTFTDLSYPGLELSYFSTKDNRALIGARVTQGDYVLGKAVRLDASPRQVFLELGPPR
                     FQNGTVFGWTDEAEYASLEVTFSGGKVLLVDFSVEPD"
     gene            1881..>2677
                     /locus_tag="P5219_04660"
     CDS             1881..>2677
                     /locus_tag="P5219_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRX26077.1"
                     /translation="MPALHRSDAQSIARAVILAAALLLVPPALPGAAARQEGPDILAL
                     LEERRREIAALMERAVVYVLVDHGDSASMGTGFVVAEGYVVTNAHVVDGKGTIILANP
                     VQKPVRAKLVRREYGGEAGGNDFALLRFSPDVPLPVLVFSPRVSRMDRVSSWGFPLLV
                     TQFDQSIDEILNGELRTAPPVVYTEGTVSAIVEKQGRRNIIHTAAIASGNSGGPLINS
                     RGEVVGINTWGYTEEDEGAFVNASLPADRIMDFLRRAGVTPKASSQGP"
BASE COUNT          539 a          862 c          804 g          472 t
ORIGIN      
        1 ccatgagccg ggcgaggtcg tccgaggcgc cgcccctcat ccggaggttg ttcttcagat
       61 tggcgaagat gaagacccgc tccacaggat ctgtgacatc ccggtagccc acctccccgt
      121 tttccaccac gatctcctgc agcctccccc ttttgaggga gtccatctcc gcccagtccg
      181 cggcgggctg ggtgatgtac acgtcgaggg tggcctgccg gtgggcggtc accagggcgt
      241 ccagtgccgt catgtccagg ctgaagctct cccggatggc ggaagagatg aaatccctga
      301 gggagaccgc atcctccacc tgggccgcca gggtggatat gtcgatgtcc ccctcgaacc
      361 ggagctctcc gtaggattca cgggaggacg cgccggcatc catgcggacg aggtagggga
      421 aatcgggatg ggcgtccctg tatcgccgga ctccctcggc aaaggcccgt tcgacaccct
      481 tccgccgtac gtccgcgaga gaccgttccg cttccccgaa ggaccgctcc catgcctcga
      541 gggctccggg aagggcgtcc gccggggaga gggcccccgt cttccgcagc tcctcacccc
      601 ggagccggat gaactcctcc ttgaaggcca tcacgtctct catggccatg ccgtcccggc
      661 cgagggaccg gaaaaactcc atgccgtcca ggtcgccggc catcacactc cggagcctcg
      721 actcgagaag acgataggtc tcggaaggct ggaccggagg tttgccgatg gggttgacct
      781 tcagccacca gctgttctcc cggtcggaag gggcggcgta ccccgcacag gagaggaaaa
      841 gaacaatcag aagcacaagg acggtcaaaa gggatcgttt catgggaaca cctccgaaaa
      901 aaagtccgga caggaagaac cggcagggaa aaaacaggcg gcccctccga agaaagggcc
      961 gctgcggtat cagtcaggtt ccacggaaaa atccaccagg agcactttcc cgcctgagaa
     1021 tgtcacctcc agggaagcgt actccgcctc gtctgtccag ccgaaaaccg tcccgttctg
     1081 gaaccggggc ggtccgagct caaggaagac ctgccggggc gaggcgtcca gtcggacggc
     1141 ttttccgagg acatagtccc cctgcgtcac cctggcccca atgagggcgc ggttgtcctt
     1201 cgtggagaaa tagctgagtt caagccccgg gtaggacagg tcggtgaatg tcactttgta
     1261 ggacgggtca ggccgcccgg gtatctccct gtcatttctg gaaaggggct ttcccagctt
     1321 ccgcacggcc tcttcggcgg tgcggccgaa agtctccgtc gtggccagga agaacgcgtc
     1381 aaaggcaagg ctctggacgc cgcatgtatt cccaaaccac tcaccctggg gaaggggttc
     1441 cacgtactgt ccgtacaccc atccctccac caccggacca tcgggaagcg gaacccggga
     1501 aatcaccagg gaccagggga aggtgtccgc accctgttcc tcccggagca ccacggcagt
     1561 ctccccgccg gaaaagcggc cgatgaccct cccggatctc gatccctcgt cacggatatt
     1621 cacggatgat cccgtgatcc tgacaaatac gggatcgacc tcggcggagg tgccggcggc
     1681 ggcgaaaagc accccgagcg cggccaggca gactatgaat tttcttctgg tgaacatcgt
     1741 ccatcgcccc ttttggaaaa atcagctttt tcagatcgtc agaaatacta tataccacct
     1801 tccgccatct gggaagattt gacactcccc atccgggaga atactatgag cccaacgact
     1861 atcaccaggg aggaaaagcc atgccagctc ttcaccgatc cgatgcacag tctattgccc
     1921 gcgccgtcat tcttgccgca gcccttttgc ttgttcctcc ggcccttccc ggagcggcgg
     1981 cccggcagga ggggccggac attctcgctc ttctcgagga gcggcgtcgg gaaatcgccg
     2041 ccctcatgga aagggcggta gtctacgtcc tggtggatca tggagattcc gcaagtatgg
     2101 gcacgggctt cgtcgtcgcc gagggatacg tggtgaccaa cgcccacgtg gtggacggga
     2161 agggaacgat cattctcgcc aacccggtcc agaaaccggt ccgggcaaag ctcgtccgca
     2221 gggaatacgg cggcgaggca ggaggaaacg actttgccct gctccgtttt tctcccgatg
     2281 tgccccttcc ggtcctcgtt ttttcccccc gggtgagccg catggacagg gtcagttcct
     2341 ggggctttcc ccttcttgtc acccagttcg accagagcat tgacgaaatc ctgaacggtg
     2401 aactcagaac cgcccctccc gtggtctata cagagggaac cgtcagcgcc atcgttgaaa
     2461 agcagggcag gcggaacata atccacaccg ccgccatcgc ctccgggaac agcggcggtc
     2521 ccctgatcaa cagccgcggg gaggtggtgg gcatcaacac ctggggctac accgaggaag
     2581 acgagggagc tttcgtgaac gcttccctcc cggctgaccg gattatggac tttctccgga
     2641 gggcgggggt aaccccgaaa gcctcgagcc agggacc
//
LOCUS       DAOTHD010000211         2658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_45698_length_2658_cov_2.871302, whole genome shotgun sequence.
ACCESSION   DAOTHD010000211 DAOTHD010000000
VERSION     DAOTHD010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45698_length_2658_cov_2.871302"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..2175
                     /locus_tag="P5219_04665"
     CDS             103..2175
                     /locus_tag="P5219_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRX26078.1"
                     /translation="MRKIGPFRSFLLWAVWVGGSMGLPAFPFAAVMAGLSAGAVLLWP
                     RSRHRAKGVGLFMFPLALGFWLIHGGALQLFLGSLVQPSRAVWALGLWLRILSVISAS
                     QLWLEYVPPPVLIRSLFAGRLPASWAFLLASPLLLAEQIRTRAAQVREAQLSRGVPVH
                     GTFLERASSLTALVFPLVLGMLNELPARSAALDMKGFGLFPRRSSLAREGETGALPAL
                     EPAPQTFNGKVAELREAAFLAPGETKPVLEIPEFSVAPGDTAFLEGGNGSGKSTLASM
                     LSGAVPEHRGGSISGNVRVLGEDVTARTCLAWSPDVQTVQQNPVLSLSGCTFTVREEV
                     AFGPENLALPPAEIRTRVERALDLAGITHLGDRNPACLSGGEAQKLALASALAMNPRL
                     LVLDEAFSRIHPADRHSLASVLRRQAREEGTALVVLEKPGNTSSPWFSPAGHFRDGRL
                     LPGPSPVRSRRPAAGSSLFPEKKDDRPVLVIDDLSFRWKGETSPLLRSLNAVLRPGER
                     AALTGPNGAGKSTLMRLCAGLLEPECGAVLLGGVPVGSMAPGERAARIGFLFQDAERQ
                     IFHSTVAGEVLFSLRKSALSPDERMERMRQALSATGLSGKEEKHPLDLNSAERRMVAV
                     ASLSVREGDLLLLDEPTRDFDPEWQARFEEWMLSRREAIVAVSHDPEFTGRLFPVSWS
                     LGGGRLTT"
     gene            2325..2525
                     /locus_tag="P5219_04670"
     CDS             2325..2525
                     /locus_tag="P5219_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HRX26079.1"
                     /translation="MSTFVFSVPDMSCGHCKMRITKALEEAGFSGFDVSLETKTVTVE
                     AADSEAVLAAIGEAGYDAVLKQ"
BASE COUNT          417 a          874 c          833 g          534 t
ORIGIN      
        1 gacaagattg tggcctgcct cgtggcccag gccgtggtgc ggcagcttcc cgggcggatc
       61 cggagcctct tccccggtgc ggaaaacgtg gcctgagcgg acgtgaggaa aataggccca
      121 ttcaggtcct ttctcctctg ggcagtgtgg gtcgggggct ccatggggct cccggccttt
      181 ccctttgccg ccgtcatggc ggggctctct gcgggagcgg tcctcctctg gccgaggtcg
      241 aggcaccggg cgaaaggagt cggtctcttc atgttccccc tggccctcgg tttctggctg
      301 atccacggcg gcgccctcca gctttttctg gggagtttgg tccaaccgtc ccgggccgtg
      361 tgggctttgg gactgtggct ccgcatactc tccgtgatct ccgcgagcca gctctggctt
      421 gaatatgttc ctcccccggt cctgatccgt tccctcttcg ccggcaggct ccccgcctcc
      481 tgggccttcc ttctcgcaag cccgctgctg ctggcggagc agatacggac ccgggccgcc
      541 caggttcggg aggcccagct ctcccggggg gtcccggtcc acggcacttt cttggagagg
      601 gcgtcctccc tgacggcgct ggtctttccc ctggtgctgg gcatgctgaa cgaactgccc
      661 gcaaggagcg cggccctcga catgaagggc ttcggactct tcccccgcag gtcgtccctt
      721 gcgcgggaag gggaaacggg agccctgccc gccctcgaac cggcccctca gaccttcaat
      781 ggaaaggtgg ctgaattgag ggaggccgct tttcttgccc cgggagaaac aaaacccgtc
      841 ctggagattc cggagttttc cgttgcgcct ggagacaccg cattccttga agggggcaac
      901 gggagcggaa aatccaccct ggcctccatg ctctccgggg ccgtgccgga gcacaggggc
      961 ggatccatct ccggtaacgt ccgggtgctc ggggaggacg tgacagcgag gacctgtctc
     1021 gcctggtcgc cggacgtgca gaccgtgcag cagaatcctg tcctgtccct ctccggatgt
     1081 accttcacag tccgggagga ggtggccttc ggccctgaaa acctggcgct ccctcccgcg
     1141 gaaatccgga cccgggtgga acgggccctc gatctcgcag gcatcaccca tctcggggac
     1201 cggaacccgg catgcctctc cggaggggaa gcccagaagc tggccctcgc ctccgccctg
     1261 gccatgaacc cccggctgct ggtcctcgac gaggcctttt cccggattca tcccgccgac
     1321 cggcactccc tcgcctccgt tctccgccgg caggcccggg aggaggggac agctcttgta
     1381 gtcctcgaga agccggggaa cacctcttca ccgtggtttt caccggcagg gcatttccgg
     1441 gacggccgtc ttctccccgg cccctccccc gttcgctcca ggcggccggc ggcaggttct
     1501 tcacttttcc cggaaaagaa ggatgatcgt ccggtgctgg ttatcgacga tctttccttc
     1561 cggtggaagg gggaaacctc tcccctcctc cggtcgctga acgccgtcct ccgcccggga
     1621 gagcgggccg ccctcacggg accgaacggc gccgggaaat caacccttat gcgtctgtgt
     1681 gcggggcttc tcgagcctga atgcggagcg gtgctgctgg gaggcgtgcc cgtgggctcc
     1741 atggctcccg gagaacgggc cgcccggata ggctttctct tccaggacgc cgaacggcag
     1801 atcttccact ccacagtggc cggggaagtg cttttctcac tcaggaagtc tgccctgtca
     1861 ccggacgagc gcatggagcg catgcgacag gccctctccg cgacggggct gtccgggaag
     1921 gaggagaagc accccctcga cctgaactcc gcagagcgga ggatggtggc ggtggcctcc
     1981 ctttccgtcc gggaggggga tctcctgctg ctggacgagc ccaccaggga tttcgacccc
     2041 gaatggcagg cccggttcga ggaatggatg ctctcccgcc gggaggccat cgtggcggtc
     2101 agccacgatc ccgagttcac cggacggctt tttcccgttt cctggagcct gggcggcggc
     2161 cgcctcacaa cctgatttcc cctaccgcac cgcttttcct gcatatatta cttattttgt
     2221 aggaattctt ccccgccctc tggcattcca tacccgtgat ggtatataat tttcttcggg
     2281 aaagtttatg atatcgaaca tcattgccga ggagggatca tcctatgagc acatttgtgt
     2341 tttccgtacc ggacatgtcc tgcggccact gcaaaatgag aatcaccaag gctctcgagg
     2401 aagcggggtt ttccggtttt gacgtttcgc tggagacgaa gacagtgacc gtcgaggctg
     2461 ccgacagcga ggccgttctg gccgccatcg gcgaagcagg gtatgacgcc gtcctgaagc
     2521 agtaggcggt ccggcccgac agttcacaga cgcccccttt ctttgcggaa aggggctttt
     2581 cttttccgga ggttgccatg aacgaaacga aagagcagca gttcaaaacg agcctcaccg
     2641 tcacggggat gacctgtg
//
LOCUS       DAOTHD010000212         2657 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_45717_length_2657_cov_3.194081, whole genome shotgun sequence.
ACCESSION   DAOTHD010000212 DAOTHD010000000
VERSION     DAOTHD010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45717_length_2657_cov_3.194081"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="P5219_04675"
     CDS             complement(<1..591)
                     /locus_tag="P5219_04675"
                     /inference="COORDINATES: protein motif:HMM:NF028509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HRX26080.1"
                     /translation="MAERPKIPADNMVQAVQSAGERVFLRTFLQTMAPVSLLLVILFA
                     VIFGVLLPLLEKSHMAEKRNLCRNLILVQLHYLDALHREELAGKIPPGEGRVRALARI
                     RSLRFGEEDKDYFWILGPERTLLMHPYRPDLEGVDPDTAAGPDGFILRQLFDRIETTV
                     SSPEGGGIMDYQWHFKDRLNTLAPKTSYVALFKPWNW"
     gene            complement(773..1510)
                     /locus_tag="P5219_04680"
     CDS             complement(773..1510)
                     /locus_tag="P5219_04680"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRX26081.1"
                     /translation="MMESPLAFLAGEREKNSGPVSRTLTLALFDLDGTLIDSEENHYK
                     SDEILLARRGISFSREDKAAYVGKDIYEMVRRIRANYGLEDDVQALVDEKNALYRKVA
                     LISSRLYPPMRPLLEGLAERKLPMAVATGSNSSIASEILEVLGVRSFFTHIVSSSEVR
                     RGKPAPDIFLEAARRMNADPESAIVFEDTKYGVEAALNAGMTCVALPAPGERTDDPLF
                     RRVSYLVPGGPDALDTDDFFRWFDPLL"
     gene            1633..1998
                     /locus_tag="P5219_04685"
     CDS             1633..1998
                     /locus_tag="P5219_04685"
                     /inference="COORDINATES: protein motif:HMM:NF016050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HRX26082.1"
                     /translation="MIIETEKHAFEVDLCGGSAARNVVKTLPQEISMARWGDEFYGTL
                     KEKVECGPDTLQDVFEIGDVALWPAGNALCIFFGPTPASSGDEPRMASPGVLVGRITG
                     DATVLRDCGRSLKKVRIMP"
     gene            complement(2091..2372)
                     /locus_tag="P5219_04690"
     CDS             complement(2091..2372)
                     /locus_tag="P5219_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26083.1"
                     /translation="MKKILAAVLVVILAASVASTALASVFDGGSSFENSISLGKGNGN
                     GGGNGGGNGGGNGKGPGDGTGNGGNGPRDGTGYGPGDGTNPDCPVLPKK"
BASE COUNT          521 a          770 c          830 g          536 t
ORIGIN      
        1 ccagttccac ggtttgaaaa gggccacgta ggatgttttg ggggccaggg tgttcagtct
       61 gtccttgaag tgccactggt agtccattat tcccccccct tcgggggagg agacggtggt
      121 ttcgatccgg tcgaagagct ggcggaggat gaagccgtcc ggaccggcgg cggtgtcggg
      181 gtccacgccc tccaggtcgg gccggtaggg gtgcatgagg agggtccttt cggggccgag
      241 aatccagaaa taatccttgt cctcttcccc gaaccggagg gagcggatgc gggcgagagc
      301 ccggacccgg ccttcccccg gggggatctt ccctgcgagt tcctcccggt gcagggcgtc
      361 cagataatgg agctgcacca ggatcagatt tctgcagagg ttcctttttt ccgccatgtg
      421 gcttttttcg aggaggggga gcagaacgcc gaaaatcacg gcgaaaagaa tgaccaggag
      481 gagagaaaca ggagccatgg tctggaggaa ggtgcgaaga aacacccgct ctccggcgga
      541 ctgaacggcc tgtaccatgt tgtcggcggg aatcttcggt ctttccgcca tgagaggctc
      601 tccttccggg tcgggggccc ggaggaaaac cttttccggg ccgttccggt attatatcag
      661 gaaaaaaatg cctcaggcag aatctattgt tctcaatact gctgggtcct gaggccccgg
      721 ggggccggaa aagaaaggcc ctccccggag attccccgga gagggccttt ggttacagaa
      781 gggggtcgaa ccaccggaaa aagtcgtcgg tgtccagggc gtcggggcct cccggaacga
      841 ggtatgagac tctccggaag agaggatcat ccgtcctctc cccgggagcg ggcagggcca
      901 cgcaggtcat tcccgcgttc agggctgcct ccacgccgta cttcgtgtcc tcgaagacga
      961 tggccgactc gggatcggcg ttcatcctcc gggcggcctc gagaaaaatg tccggggcgg
     1021 gcttcccccg ccggacttcc gacgaggaga cgatatgggt gaaaaaagac cggacgccca
     1081 gaacctccag gatctccgag gcgatggagc tgttggatcc ggtggccacc gccatgggaa
     1141 gtttccgttc cgcgaggccc tcaaggaggg gtctcatggg cgggtaaagc ctgctcgaga
     1201 tgagggcgac ctttctgtac agggcgtttt tttcatccac gagggcctgc acgtcgtctt
     1261 ccaggccgta gttcgccctg atgcgccgga ccatctcgta gatgtccttc cccacgtagg
     1321 cggccttgtc ctccctggaa aaggagatgc ccctccgcgc gaggaggatc tcgtcgctct
     1381 tgtaatggtt ctcttcactg tcgatgaggg tgccgtccag gtcaaaaagg gcgagagtca
     1441 gcgttcggct gacggggccg gagttttttt ccctctctcc ggcgagaaaa gccaggggag
     1501 attccatcat gtagtgtcca cattcccttc gcttccgttt tccagaattg taccacggat
     1561 cccttttcag aaaacagcct ccgccggtag tatagaatga atggagaaga atgaaaaagg
     1621 aggtttctgc ccatgatcat cgaaacggaa aagcatgcct tcgaggttga cctctgcggc
     1681 ggctccgcgg cccggaacgt ggtgaagacc ctgccccagg agatttccat ggcccgctgg
     1741 ggggacgagt tctacggcac cctgaaagaa aaagtggagt gcggccccga cacgctccag
     1801 gacgtttttg aaatcggcga cgtggctctg tggccggcag ggaacgccct gtgcatcttt
     1861 ttcgggccca cgccggccag cagcggggac gaaccgagaa tggcctcccc gggagtcctc
     1921 gtgggcagga tcacaggcga cgcgactgtg ctgcgggact gcggccggtc gctgaaaaag
     1981 gtccggataa tgccctgata cccgaaaccc ggaggggaat tcaggaagaa aaaacggagc
     2041 ggggggaaat ttttccctcc gctccgttat taaatgcagc ctcttccggc ctatttcttc
     2101 ggcaggaccg ggcagtcggg attggtcccg tcgccggggc cgtaccccgt gccgtccctg
     2161 gggccgtttc cgccgttgcc ggtgccgtct ccgggccctt tgccgtttcc gccgccattg
     2221 cctccgccgt tgcctcctcc gttcccgttg cccttgccga gcgaaattga attctcaaag
     2281 gatgatcccc cgtcgaaaac ggacgcgagg gctgtggaag cgacggatgc cgcaagaatc
     2341 actaccagaa ctgccgccag tatttttttc atgagtgaat cccttctttc atcaggctga
     2401 aatcgagtac gcccgaataa tagcccttct ttcgggaaga cacattgtca ccggtatcaa
     2461 ttatccgccc ggtaattccc gcagttattt ttacgacggc accaccaggg gaacgccctt
     2521 gtgccggacg atgtccacgg gcacgccgta aacctcggcg atgatttctc ccgggacctc
     2581 cgacggaagg aacatccccc ggatgcatcc gtccttcagg aagagcagcc tgtcggcgta
     2641 ccggaaggcg gtgttca
//
LOCUS       DAOTHD010000213         2655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_45747_length_2655_cov_4.021154, whole genome shotgun sequence.
ACCESSION   DAOTHD010000213 DAOTHD010000000
VERSION     DAOTHD010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45747_length_2655_cov_4.021154"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="P5219_04695"
     CDS             complement(<1..210)
                     /locus_tag="P5219_04695"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HRX26084.1"
                     /translation="MPFLNSVLDQFGKKYGSSPAVRNFPVNPPLLEQVTGDSREVVPG
                     SVFCCVRGEKRDGLDFAAAAVEKGAC"
     gene            complement(215..1897)
                     /locus_tag="P5219_04700"
     CDS             complement(215..1897)
                     /locus_tag="P5219_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRX26085.1"
                     /translation="MLLKRFGGSPWFLVFLFFAVLFWRVASLQLFPDPRVEKQSRRQY
                     WSRVPVSTNRGSILDANGNALALSIPSSSFFLDPAFWDPSNGSKLRNILPDSIVATIS
                     SAMPGRFFWLARKVDEETAAKIRTLNLKGIYEISEKKRLYPNRSLLSHVLGFCDVDDR
                     GLSGIEMVWDKVLFSPPGIKILAKESSGRTLDISRHSDEGIPAGPGSVRLTIDSRIQF
                     IVEKRLDEGIAEHGAKWGSIVCMDPETGAIVAMASWPSFDPNVRDNLTNMKRIANNST
                     GRTYEPGSTFKPIVLGIAMEKGSVRGSESFNCPYRIKVADGHISEASNRSYGRLSVSE
                     ILIKSSNTGMAQIGLRVKPFDMYHGVREWGFSKPLEVELNGVEDGLLATPEQWRGVVP
                     SNISIGQGLAVTPLHLANAMAAIANGGFLLRPFIVSEVKDSTGRIIYKGERKLVREVL
                     SPKTASWLSSIMNESVKSGTGKMARLPYVSIAGKTGTAQVAEKGEYKKGKWVSSFAGF
                     WPEENPDYVMLVVIGEPSKGKYYGGDVAAPVFRRIVEDMFQTGLFACIGWGG"
     gene            complement(1930..2229)
                     /locus_tag="P5219_04705"
     CDS             complement(1930..2229)
                     /locus_tag="P5219_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26086.1"
                     /translation="MYSFRLECRLNSINSQIESFQAQQISLKQNLSALLSPGRVYGYS
                     KKQLGMTYASNVKTLRLDGPLLADGAPAVNSGGEAAGKESEGWFYFFLEKAMAGE"
     gene            complement(2343..2648)
                     /gene="mraW"
                     /locus_tag="P5219_04710"
     CDS             complement(2343..2648)
                     /gene="mraW"
                     /locus_tag="P5219_04710"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HRX26087.1"
                     /translation="MFQALRIAVNDELAVLEKGLSGAFELVRPGASVAVVSYHSLEDR
                     IVKHFFREKQKENLGTIETKRPLIPSDEEIERNYKARSAKLRLFVTNEPGKETKPKW"
BASE COUNT          635 a          697 c          659 g          664 t
ORIGIN      
        1 gcatgccccc ttttctaccg ctgcagccgc aaaatcaagc ccgtctctct tttctcccct
       61 gacacagcaa aaaacggaac ctggaacgac ttccctgctg tctcccgtga cctgctccag
      121 taatggcggg ttcaccggaa aattccggac ggccggcgat gaaccgtatt tttttccgaa
      181 ttgatccaga accgagttca agaacggcat atggctatcc tccccaaccg atgcatgcaa
      241 aaagaccggt ctggaacata tcttccacta tacggcggaa aacaggggcg gcaacatcgc
      301 cgccgtaata tttccccttt gaaggttctc ctatgaccac aagcatgaca tagtccgggt
      361 tttcctcagg ccagaagccg gcaaaagacg aaacccactt tccctttttg tattctccct
      421 tttccgccac ctgggccgtc cctgtttttc cggcgatgga aacataggga agccttgcca
      481 tcttgcctgt accgcttttt accgattcgt tcataatcga tgagagccag gaggcggttt
      541 tcggagagag gacttcgcga acgagtttac gttcaccctt gtagataatc cggcctgtcg
      601 aatccttgac ttcggataca ataaagggcc tgaggagaaa acctccgttg gctatagccg
      661 ccatggcgtt ggccaggtgg agaggcgtga ccgcaagtcc ctggcctatt gaaatattcg
      721 aaggaacaac ccctctccat tgttccggtg tggcgagaag tccgtcttcc actccgttga
      781 gctccacttc aagcggtttg ctgaagcccc attcccgtac tccatggtac atgtcgaagg
      841 gcttgacccg caggccaatc tgggccatgc ccgtattcga ggatttgatg aggatttcgg
      901 aaacggagag tcttccgtaa cttctgttgc tcgcttcgga aatatgacca tcagcaacct
      961 tgattctgta aggacagttg aaactttcgg atcctctgac agaacctttt tccatggcaa
     1021 tccccagaac gatgggtttg aatgtggacc ccggctcgta cgtgcgcccc gtggagttat
     1081 tcgctattct cttcatgttt gtcaggttgt ccctcacgtt ggggtcgaaa gacggccagc
     1141 tggccatggc gacgattgcc ccggtttccg ggtccataca gacaatgctc ccccacttgg
     1201 ctccgtgttc cgcgatgcct tcatccagac gcttctcaac aatgaactgg attctcgaat
     1261 ctatggtaag ccggaccgat cccggtcctg cagggatacc ttcgtcggaa tggcggctga
     1321 tatcgagggt acgtccagac gactccttgg ccagaatttt gattccggga ggagaaaaaa
     1381 gcactttgtc ccacaccatc tctattcctg acagccctct gtcgtccaca tcacagaatc
     1441 caaggacatg ggaaagaagg cttctgttag gatacaggcg cttcttttcg cttatttcgt
     1501 aaattccctt gagattcagt gtgcggattt tcgctgctgt ttcttcatcc acttttctgg
     1561 caagccagaa gaaacggccg ggcattgccg atgaaatggt ggcgacaatg ctgtcaggaa
     1621 gtatgttccg gagtttggaa ccgtttgagg gatcccagaa ggcgggatcg aggaagaaac
     1681 ttgaagaagg aattgacaaa gcaagggcat ttccgttggc atcgagaata gaacccctgt
     1741 tggtgctgac ggggacacgg ctccagtatt gccgccgtga ttgtttctcc acgcggggat
     1801 cgggaaaaag ctggagagag gctactctcc agaaaagaac cgcaaaaaaa agaaaaacaa
     1861 gaaaccaggg ggaaccccca aagcgcttta gcagcatgtt cagtccctcc gaggcgcgtc
     1921 acaaggcgat cattctcctg ccatcgcttt ttccaggaag aagtagaacc agccttccga
     1981 ttcttttccg gcagcttctc caccactgtt gaccgccggg gccccatccg cgagaagagg
     2041 tccgtccagg cgaagggttt tgacatttga agcatacgtc atgccgagct gtttttttga
     2101 atagccatac actcttcccg gagaaagaag agccgacagg ttctgtttga gagagatctg
     2161 ctgtgcctga aaggattcaa tctgggagtt tatgctgttg agcctgcatt caaggcgaaa
     2221 actgtacaaa cgaagaatcc caagtcccat gaacgatccg aaaacgacaa cggcagaaaa
     2281 aataagataa ggacaaattt ttctcaagga aaaagtggga actgcctgat catctctctg
     2341 actcaccatt tgggttttgt ctcctttccc ggctcgtttg tgacgaaaag ccgcaatttt
     2401 gcgcttctgg ccttgtaatt tctctctatt tcttcgtctg aaggaataag agggcgtttt
     2461 gtttcgatgg ttcccaggtt ttccttttgt ttttctctaa aaaaatgttt tacaatgcgg
     2521 tcttcgagag aatggtagct tacaacagct acggaagcgc cgggacgtac cagttcaaaa
     2581 gctccggaga gtcccttttc aagaacggca agttcgtcat tgacggctat ccggagggcc
     2641 tggaacactt ttcgt
//
LOCUS       DAOTHD010000214         2655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_45750_length_2655_cov_3.695769, whole genome shotgun sequence.
ACCESSION   DAOTHD010000214 DAOTHD010000000
VERSION     DAOTHD010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45750_length_2655_cov_3.695769"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="P5219_04715"
     CDS             complement(<1..285)
                     /locus_tag="P5219_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HRX26088.1"
                     /translation="MKGFGAAISAISYYLPPKVVDNRALVEEFGTWTEDKIYQKTGIR
                     ERHVVDGELVSDLAARAAEKLFEEHGVDRETIDFLLLCTESPDYYLPATAC"
     gene            538..1833
                     /locus_tag="P5219_04720"
     CDS             538..1833
                     /locus_tag="P5219_04720"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HRX26089.1"
                     /translation="MSGRKISFVHCADLHLDSPFSGLSDISPSLGAFLRKATFRAFEN
                     AVTFALKKGADFFVVSGDVYDSENRSVRAQVFFLEQLRRLSGGGIPSFIAAGNHDPLS
                     GWEADRDFPPLAFRFGPGVESVPLLLGGERAGTVHGFSYPVREVEENMALRFSGGKGE
                     GVNIALLHCNVGGRKGHENYAPCSLDDLRAAGMDYWALGHVHGAEVLCRDPLVVYPGT
                     IQGRNIRETGEKGVFSVTLFPGEPGCAEFIPCDVVRWRSEDISIDGMERDEEFFQAVS
                     DLKETVRRENGGLPALLRLSVSGRGKIHGLLRRPGFLRGPGGLLETMNEGEEDRPDFV
                     FTEEISGVTLPPLDLDSLAAGSHFAGDFLKEIRAFRQRGDLREGLKDILRKRGILDRI
                     QSRDVLEVIDSLSDEDTELLLDRGTWSALSGLLEGIDGV"
     gene            1830..>2655
                     /locus_tag="P5219_04725"
     CDS             1830..>2655
                     /locus_tag="P5219_04725"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRX26090.1"
                     /translation="MKITDFFVSGFGIFSGSGARLSGGLTVFQGENESGKTTLMNFFR
                     RLLFSRGKNGRGKTNLYEPQRGGQHGGSARIRMEDGREYILSVEGQKNYIAPADGGPA
                     AELSPDFFSISREVYESVFAMGLGEMQSLDPLNSSDVAARFFAAGAGLGSASLPRLLS
                     VLEARQNELYRPWGNARSASTVNRLLASMAETDGAVRDLRELNGSWRRMKDDLSAMEA
                     SMEEKKERLDVLKRRTARLELLEKARPSREALVETERKLAGLEGLHPFPDDGLSRLE"
BASE COUNT          491 a          750 c          884 g          530 t
ORIGIN      
        1 gcaggcggtg gcgggaagat agtagtccgg gctttcggtg cacagcagca ggaaatcgat
       61 ggtctcccgg tcgacgccgt gttcttcgaa gagtttttcg gctgccctgg ccgcaaggtc
      121 cgacaccagc tccccgtcga ccacgtggcg ttccctgatt cccgttttct ggtatatttt
      181 atcttcggtc cacgtgccga actcctccac gagggccctg ttgtccacca ctttcggcgg
      241 aaggtaatac gaaatggccg aaatggccgc tccaaatcct ttcatctctc gacaatcctc
      301 cacaatcgga ttcaattaca gaagaattat cccattgtca ccggctggtg acaagtcccg
      361 ggagggaaag ggtgcgaggg gccacgcttt gccgggaaaa gctgcggatt cagggggaca
      421 ggatcgtgtt gcaaagaaga ggggtgtggc ataaagtaga cgggattttt gaagggagtg
      481 gtgccgagtg taccagtaca agttgccttt tctcccggcg gagaggggga agacccggtg
      541 agcggccgga aaatttcttt cgttcactgc gctgaccttc acctcgacag tccgttttcc
      601 gggctttccg atatttctcc ctccctcgga gcattcctga gaaaagcgac attccgggct
      661 tttgagaacg ccgtcacctt tgccctgaag aagggggcgg attttttcgt ggtctcgggc
      721 gatgtctacg acagcgagaa caggagcgtc cgggcccagg tcttctttct tgaacagctc
      781 aggcgccttt ccggcggggg gattccctct ttcatcgctg ccggaaacca cgatcccctg
      841 tcggggtggg aggcggacag ggattttccg cccctggcct tccgcttcgg ccccggggtc
      901 gagtcagtcc ccctgctgct tggaggggag agggcgggca cggttcacgg tttcagctat
      961 cccgtccggg aggtggagga gaacatggcc ctccgttttt ccggcgggaa gggagagggc
     1021 gtgaacatcg ccctgctcca ctgcaacgtg ggcggccgga agggccatga aaactacgct
     1081 ccctgctccc tggacgacct gagggctgcg ggcatggact attgggccct ggggcacgtg
     1141 cacggggcgg aagtgctctg ccgggacccc cttgtggtct atcccggcac tatccagggg
     1201 aggaacatcc gggagacggg ggaaaagggc gttttttccg tcaccctgtt ccccggtgaa
     1261 ccggggtgcg ccgaattcat tccctgcgac gtggtccggt ggagaagcga agacatttcc
     1321 atcgacggta tggaacggga cgaggagttt ttccaggccg tgtcggacct gaaagagacg
     1381 gtgcggcggg aaaacggcgg gctgccggcc cttctgcggc tttccgtttc cgggcggggg
     1441 aagatccacg ggcttctccg gcgtccgggg tttctccggg ggcccggcgg actcctggag
     1501 acgatgaacg aaggggagga ggacaggccc gattttgttt tcaccgagga gatttccggg
     1561 gtgacgttgc cccccctgga tcttgattcc ctcgctgcgg gcagccattt cgccggcgat
     1621 tttctgaagg agatccgggc cttccggcag aggggcgatc tccgggaggg actgaaagac
     1681 atcctccgga aaaggggtat tctggacagg atccagtcca gggatgtgct ggaagtgata
     1741 gactccctct ctgacgagga cacggagctg ctcctcgaca gggggacctg gtcggccctg
     1801 tccggactgc tcgaagggat tgacggagta tgaaaataac cgattttttt gtgagcggct
     1861 tcggcatctt ttcaggatcg ggagcgaggc tgtccggggg tctcacggtc ttccagggag
     1921 agaacgaaag cggcaagacc accctgatga atttcttccg ccgcctgctt ttcagcaggg
     1981 gaaagaacgg cagggggaag acgaacctct acgagcccca gcggggcggt cagcacggag
     2041 gctccgccag gatccgcatg gaggacggaa gggaatacat tctttccgtg gaggggcaga
     2101 agaactatat cgctcccgcc gacggaggtc ccgccgccga attgtccccg gacttcttct
     2161 ccatcagcag ggaggtctac gagagcgtgt tcgccatggg actcggggag atgcagtccc
     2221 tggaccccct gaattcttcc gacgtggccg cccggttctt cgccgccggg gcgggcctgg
     2281 ggtcggcgtc ccttccacgg ctgctctcgg tccttgaggc ccggcagaat gaactgtacc
     2341 gtccctgggg aaacgcgcgg agcgcatcca cggtgaaccg gcttctcgcc tccatggccg
     2401 agacggacgg tgccgtgcgg gacctccgtg agctgaacgg aagctggcgc aggatgaagg
     2461 acgacctgtc agccatggaa gcctccatgg aggagaagaa ggagcggctg gacgttctga
     2521 aaagacggac cgccaggctg gagcttctcg aaaaggcccg tccttcccgg gaggccctgg
     2581 tcgagactga acggaagctc gccggattgg aggggctcca tccttttccc gacgacggcc
     2641 tgtcccggct ggaac
//
LOCUS       DAOTHD010000215         2653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_45792_length_2653_cov_3.623557, whole genome shotgun sequence.
ACCESSION   DAOTHD010000215 DAOTHD010000000
VERSION     DAOTHD010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45792_length_2653_cov_3.623557"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1201)
                     /locus_tag="P5219_04730"
     CDS             complement(<1..1201)
                     /locus_tag="P5219_04730"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRX26091.1"
                     /translation="MNRFLRRIDQWRLLLETGAVPGMAVPGLIYADDYIESLLKGEDA
                     LSQVANVACLPGIVGYSMAMPDIHQGYGFPIGGVAAFDVSSGVISPGGVGYDISCGVR
                     LLASKIPAEEFRPRADRVLAALFSAVPCGVGSVKDSLGTKEVDRILEEGAVRVVRSGR
                     GTEGDIRRTEEGGCLVGAEPGAVSPRAKERGKGQLGTLGSGNHFIEVQETEELFLPET
                     AASMGLSKGCVAVMIHCGSRGLGHQVCDDYLKVMGRAMSKYGIAVPDRQLCCAPVDSP
                     EGRQYLGAMKAAANFAAANRQAIAEAVRDVFAGFFPGEKLATVYDVSHNLASIENHVW
                     EGRKRQLCVHRKGATRAFNGLPVLIPGSMGTASYVLEGTPGAERETFGSACHGAGRVL
                     GRNEAIRR"
     gene            complement(1218..1628)
                     /locus_tag="P5219_04735"
     CDS             complement(1218..1628)
                     /locus_tag="P5219_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HRX26092.1"
                     /translation="MAWKILDHTADAGFEAEADTLAEIFLDAAEAFLFIAAGPAPGDF
                     SETGEFHTFFLSAVDGEELAVSWINELIFIAETRSEWFLPLQVRVSLSPPSLSARGKT
                     VALKGRALSVKAATYGGLQLRTSPRPFLRMYFDL"
     gene            1735..2178
                     /locus_tag="P5219_04740"
     CDS             1735..2178
                     /locus_tag="P5219_04740"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRX26093.1"
                     /translation="MEKTRQIIRSIGGIHDSIHRFISGYIEECEKECLVPSHGELLTA
                     LFSGREFTMGDLAREIRKTKPTVTVLVEKLVRCGYVTRERDARDGRISYIRLTEKGWG
                     LEPVLEDILGFLEKRFFSGFTVQEKNNLEELLFRIEENLKEEKRI"
     gene            2175..>2653
                     /locus_tag="P5219_04745"
     CDS             2175..>2653
                     /locus_tag="P5219_04745"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRX26094.1"
                     /translation="MKDFFAAVKDRRTYYGISKASPVSDDRIKEIVHFAVKHVPSAFN
                     SQSGRAVLLLGKSSDEFWSMVKESLRKIVPADKFAPTEEKINGFGSGYGTVLFFEDSR
                     PVKNLQEQFPSYAAHFPVWSDHSSGMLQYIVWAGLESEGFGASLQHYAPVVEDDVRK"
BASE COUNT          576 a          752 c          755 g          570 t
ORIGIN      
        1 ttctccggat ggcttcgttc ctcccgagca cccttccggc accgtggcac gccgacccga
       61 aggtttccct ttccgccccc ggcgtgcctt ccagaacgta gcttgccgtg cccatgcttc
      121 cggggatcag cacaggaagt ccgttgaagg cccgggtggc tcctttccgg tgaacgcaga
      181 gctgtctctt tcttccctcc cagacatggt tttctatgga tgcaaggttg tggctcacat
      241 cgtagacggt ggcgagtttt tcccctggaa agaacccggc aaagacgtcc cgtacggctt
      301 cagcaatagc ctggcggttg gccgcggcga aattcgccgc ggctttcatg gccccgagat
      361 attgccgccc ttccggtgag tccacggggg cgcagcaaag ctgccggtca ggcacggcga
      421 tcccgtactt cgacatggct ctccccatga ccttcaggta atcatcgcaa acctggtgcc
      481 caagcccccg gcttccgcag tggatcatca cggcgacgca tcctttggaa agccccatgg
      541 aggccgctgt ttcggggagg aacagttcct ccgtttcctg gacttcgata aagtgattgc
      601 ctgaacccag ggtgccgagc tgccctttgc cccgttcctt tgcccgagga gaaacggcgc
      661 ccggctcggc ccccacgagg catccccctt cctccgtccg ccggatgtcg ccttccgttc
      721 ccctgccgga gcggacgact ctgacagctc cttcctcaag gatcctgtcc acttccttcg
      781 ttccgagcga atccttcacg gatccgacgc cgcatgggac tgcggagaaa agggccgcca
      841 gcacccgatc cgcccgggga cggaattctt ccgccgggat ttttgaggcg aggagcctga
      901 caccgcagga aatgtcgtat cctacccctc cgggggatat gaccccggag gaaacgtcga
      961 aggccgccac tccgccgatg ggaaaaccgt acccctggtg gatgtccggc atggccatgc
     1021 tgtaccccac aataccggga aggcaggcca cgttggccac ctgggagagg gcatcctctc
     1081 ccttcagaag cgattctatg tagtcatcgg cgtagatgag ccccggtaca gccattcccg
     1141 gcaccgcgcc ggtttcgagg agaaggcgcc attggtcgat gcgcctgaga aaacggttca
     1201 ttctgttcac ctcgctgtca gagatcgaaa tacatcctca gaaaaggacg gggagaagtc
     1261 ctgagctgga gccctccgta cgtggcggcc ttgacggaga gagcccggcc tttgagggca
     1321 acggtttttc cccgggctga aagggacgga ggagagaggg aaaccctgac ctgcagggga
     1381 agaaaccatt ccgagcgtgt ctccgctatg aagatcagct cgttgatcca ggagaccgcc
     1441 agttcttccc cgtccactgc ggagagaaag aaagtgtgga attcacctgt ttcggagaaa
     1501 tcaccgggcg ccggtcctgc ggcaatgaaa aggaacgctt cggctgcgtc gaggaagatc
     1561 tcggccaggg tatccgcttc cgcttcgaat cccgcatccg ctgtgtgatc cagtattttc
     1621 caggccacag gctttccgac ctcccttttc cggaatgttc ccggaattct catgttacca
     1681 taaaaataag aagggctctt gacaaaggtt agatgtcaaa ctaatatgag tgccatggaa
     1741 aaaacacggc agattatccg cagtatcggc ggaattcacg attcaataca ccgtttcatc
     1801 tccggatata tagaagaatg tgagaaagaa tgccttgttc cctcccacgg agagcttctt
     1861 actgctcttt tttcagggag agagttcacc atgggtgacc ttgcccggga gatccggaaa
     1921 acaaagccca cggtgactgt gctcgtggaa aagcttgtcc ggtgcgggta cgtcaccagg
     1981 gaacgggatg cacgggacgg gcgaatttcc tatatacggc tcaccgaaaa aggctggggg
     2041 ctggaacctg ttcttgaaga tattttaggt ttcctcgaaa aaagattttt ttcaggtttt
     2101 actgtgcagg aaaaaaacaa tctggaggaa ttgctgttcc ggattgaaga aaatttgaag
     2161 gaggagaaga gaatatgaag gatttttttg cggcggtaaa ggaccggagg acctactacg
     2221 gcatttccaa agcgagcccc gtttctgacg acaggataaa ggagatagtt catttcgcag
     2281 tgaagcatgt tccctcggcg ttcaattccc agagcgggag ggcggtgctc ctcctcggga
     2341 aatccagcga cgaattctgg tccatggtaa aggaatctct caggaagatc gttcctgcag
     2401 acaagttcgc ccccacggag gaaaagatca acggtttcgg aagcggctac ggtacggtgc
     2461 tcttcttcga ggattcgcgg ccggtcaaaa acctgcagga gcagttcccc tcctatgccg
     2521 ctcatttccc cgtgtggtcc gaccattcat cgggaatgct tcagtatatt gtctgggccg
     2581 ggctggaatc agagggattc ggtgcctcgc tgcagcatta cgctccagtg gtggaagatg
     2641 atgtcaggaa gaa
//
LOCUS       DAOTHD010000216         2650 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_45871_length_2650_cov_3.613873, whole genome shotgun sequence.
ACCESSION   DAOTHD010000216 DAOTHD010000000
VERSION     DAOTHD010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45871_length_2650_cov_3.613873"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2650
                     /locus_tag="P5219_04750"
     CDS             <1..>2650
                     /locus_tag="P5219_04750"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRX26095.1"
                     /translation="TERSGKKGPFRPGTDTDVQLRGKDLTAGDVERARREYNARVEDY
                     LRKNGLSVEKGINWAARTETDIMPSPADMKNPGEFARAAAYINGDGGNMYASPLAAGA
                     QGKLDRGDSVGIAEGRAYAEEMKSRIDAMKREKASLMKKYSASADPVERSFLAAEVRK
                     AESHQAKYIDRINRLERSLGDGAGLPPRDGGNAPLLDAARARDSGAASRRGEAAVDAL
                     SGHLTEKALKNYASTLGDMAAGSSSPESRSALRKAAAETLKGLPPSRLGDAISEMEAR
                     HGKDFAREMAAEMKKTAVPGEKPGPGGSGARGSTLLRGAGIASAVIGGYNALMEENRR
                     TSGNPDYGKVALELAWDMTLRGTAEAVTTRTAEYTKAEVERLREYYRSRGEDPDSPAV
                     KMKIAAGAIAKGTAYGTVRGGYELAKAAGRWTGGAIVEGAETVISLAGEALDTINVLE
                     TTSAELRAQGMAVEVQNARSVSSGRELVRELRRTASLAESLRAAMEQNVRWARSLDIW
                     RSRALDSLRGRAERLSELPSLVKVLERGAKQWGSDLKKNESDSTGLLTRIEGLAKDLA
                     AGSPVEGLEANMAMAAEDHARNRGLFERTLEEMEIIREVSEAEDLLSAFSEDMAALRE
                     AAALSREGAAAMKRNREAWQSALSEFDRVRKALRRGYAHFFEKKGIPGWMVIGRDEQA
                     VASPERNLPGEFGSWLGTLLRMEGSIASDLKKIPAPDEKTGKGSAEAARRARILAEDL
                     LPRAEGARRALEAADSALGRLLAAAASSPRAPVLAVTAPSGGEAGQELEFSAAVENAP
                     PSIVIRWDFGDGTASEGRTAIHAYTREGTYRGWVVLLLEGRQAMRKDFSVKVAPAVGA
                     GAGQPGVLPVLPGVHERIGALFGLSGI"
BASE COUNT          529 a          783 c          952 g          386 t
ORIGIN      
        1 gacggagcgg agcgggaaga agggcccctt ccggccgggg accgacacgg acgtgcagct
       61 ccggggaaag gacctgaccg ccggtgacgt ggagcgggcg agaagggaat acaacgcccg
      121 cgtggaggac tatctcagga aaaacggtct ttccgttgaa aaaggaatca actgggccgc
      181 ccggacagag acggacatca tgccatcccc ggcggacatg aagaacccgg gtgaattcgc
      241 gcgggctgcc gcctacatca acggcgacgg aggcaacatg tacgcctccc ccctcgccgc
      301 cggagcccag ggcaagctcg accgggggga ttccgtgggc atcgccgagg gaagggccta
      361 cgccgaggag atgaagtccc ggattgacgc catgaagcgg gagaaggcat ccctgatgaa
      421 aaagtattca gcctccgccg acccggtgga gcggagcttc cttgccgcgg aggtacgcaa
      481 ggcggaatcc caccaggcga agtatatcga ccgcatcaac cggctggagc gcagcctcgg
      541 ggacggtgcc ggccttccgc cccgggacgg gggaaacgct cctctcctgg acgccgcaag
      601 agcacgggat tccggcgcgg cctccaggag aggggaagcg gcggtggatg ccctgagcgg
      661 ccatcttacg gagaaggcgc tcaaaaacta cgcttccacc ctgggagaca tggcggccgg
      721 ttcctccagc ccggaaagcc ggtcggccct ccggaaggcc gccgccgaaa ccctgaaggg
      781 gctgcctccg tcccggctcg gggacgccat ttcggaaatg gaagccaggc acggcaaaga
      841 cttcgcccgg gagatggcgg cggagatgaa gaaaacggct gttcccgggg aaaagcccgg
      901 cccgggaggt tccggtgcga gaggaagcac actcctccgg ggagcgggga tcgcctccgc
      961 cgtcatcggc gggtataacg ccctgatgga ggagaaccgg cggacctcgg ggaaccccga
     1021 ctacgggaag gttgccctgg aactcgcctg ggacatgacg ctccggggaa cggccgaagc
     1081 cgtcaccacc cgaacggcgg aatacacgaa ggccgaggtg gaacggctcc gggagtatta
     1141 ccgaagccgc ggagaggatc ccgacagccc cgctgtgaag atgaagatcg ccgccggagc
     1201 catagcgaag ggaaccgcct acggtaccgt gaggggcggg tacgagttgg ccaaggcggc
     1261 gggccggtgg accggcggcg ccatcgtcga gggggcggaa acggtcatat cccttgccgg
     1321 tgaggccctc gacaccatca atgtcctcga gaccacctcc gccgagctcc gcgcccaggg
     1381 aatggcggtg gaggtgcaga acgcccggtc agtgtcctcg gggagggaac tggtgcggga
     1441 actcaggaga actgcgtccc tggcggagtc cctccgggct gccatggagc agaatgtgcg
     1501 ctgggcccgg tcccttgaca tctggagaag ccgggcgctg gattccctca ggggaagggc
     1561 tgagaggctc tccgaactgc cctccctggt gaaggttctg gagagggggg cgaaacagtg
     1621 gggctctgac ctgaaaaaaa acgagtccga cagcaccggg ctcctcacaa ggatcgaggg
     1681 gcttgcgaaa gaccttgccg ccggaagtcc cgtggaaggc ctggaggcga atatggccat
     1741 ggctgcggag gaccatgccc ggaaccgggg cctgttcgaa agaaccctgg aggagatgga
     1801 gatcatccgg gaggtttcgg aagctgaaga tcttctttcc gctttctcgg aagacatggc
     1861 ggccctccgg gaagctgcgg cgctgtcccg ggagggggcg gcggccatga agcggaaccg
     1921 ggaggcgtgg cagtccgccc tttctgaatt cgaccgggtc aggaaggcgc tccgccgggg
     1981 atatgcccac ttctttgaga agaaggggat cccagggtgg atggtcatcg ggagggacga
     2041 acaggccgtt gcctcacctg agagaaatct tccgggggaa ttcggttcct ggctcgggac
     2101 gctcctgcgc atggagggaa gcatcgcgtc cgatctcaaa aaaattcctg cccctgacga
     2161 aaagacgggg aagggatcgg cggaagctgc ccgaagggcc cggattctgg cagaggacct
     2221 tcttccccgg gctgaaggag cgcgcagggc tctcgaggcc gctgacagtg ctctcggccg
     2281 ccttcttgcc gccgcagcgt cctctccccg ggcgccggtc cttgccgtga cagccccttc
     2341 cggcggggaa gcggggcagg aactggaatt ttcggcagcg gtcgaaaatg ctcccccctc
     2401 cattgtcatc cggtgggatt tcggtgacgg aaccgcgtcc gaaggcagga cggccatcca
     2461 tgcctatacc agggagggaa cgtaccgggg atgggttgtg cttctcctgg agggaaggca
     2521 ggcaatgcgg aaggactttt ccgtgaaagt ggctccagcg gtcggcgccg gcgcaggtca
     2581 gccgggagtt ctgcccgtgc ttccgggcgt tcacgaaagg attggggccc ttttcggact
     2641 gtcgggcatt
//
LOCUS       DAOTHD010000217         2645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_45992_length_2645_cov_3.890347, whole genome shotgun sequence.
ACCESSION   DAOTHD010000217 DAOTHD010000000
VERSION     DAOTHD010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45992_length_2645_cov_3.890347"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1638
                     /gene="dnaE"
                     /locus_tag="P5219_04755"
     CDS             <1..1638
                     /gene="dnaE"
                     /locus_tag="P5219_04755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRX26096.1"
                     /translation="ADTLGVFQLESSGMRQLLKKLRVDCFEDLIAVLAMYRPGPLGSG
                     MVDQYIQCKHGRAKVEYLHPLLEDVLKETYGVVLYQEQVMQCAAILAGYSLGEADLLR
                     RAMGKKKADVMEQQRAKFVEGAKERGIDGKTAENIFNIIQEFAGYGFNKSHSAAYALI
                     TYQTAWLKANYRPEFMAAYLSSQIGSKKEDLAAYVREVRNSGIKVLPPDVNTSMASFT
                     AVGDVIRFGLGAVSKSGHTAVEAILAARNEGGPFASLWDFLCRVDLRVVNKSVVENLI
                     RAGAFDSLNDNRRQLLEGLPDLVSAASKKCADGNQCSLFELLPEETDEGGPDLPEVED
                     FSIHERLELEKDATGLYISGHPFEQHEAKVRSFSNCAIGDLKKWKGKKNPPLVGGMVL
                     AVRERTTKNGDPMGIIELEDGESRVEAVCFPKTWASLRGKISAGQVCFVSGFPEERGE
                     TSVIARSVFPVEEAGERGSPPYVRVSVFEDSLKNVSVKDLFRALKTYPGKSPVLFEIR
                     NDDYSVTMLLQDVRVDPGAPIASVVEEVLPGEMFQIG"
     gene            1702..>2645
                     /gene="pyk"
                     /locus_tag="P5219_04760"
     CDS             1702..>2645
                     /gene="pyk"
                     /locus_tag="P5219_04760"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRX26097.1"
                     /translation="MTKVKIVCTIGPACSRYETLVDMAKAGMNVARFNFSHGEYEGHL
                     GMLNLVRTVERDLKVPIACLLDTKGPEIRTGKVEGGTVSLEQGSTLTLTTRSLLGNAS
                     LVTVHYEALPREVVPGQEIFIDDGTLHLKVEEISGTEVVCRVIVGGLLGDTKGINIPG
                     AEISLPALSEKDVEDIRWGLEHQMEYIAVSFVKNRRDIMEVRRIMEEFGGSMKVIAKI
                     ETRQAVLNIDEITDVVDGVMIARGDLGVEIPTEEVPLQQKRIIDICRVKGKAVIVATQ
                     MLDSMIRNPRPTRAEASDVANAVLDGADAVMLSGETAKG"
BASE COUNT          578 a          721 c          851 g          495 t
ORIGIN      
        1 gcggacaccc tcggcgtgtt ccagcttgaa tcgtccggga tgcggcagct cctcaagaaa
       61 ctccgggtgg actgtttcga ggatctcatc gctgtcctcg ccatgtaccg tcccggccct
      121 ctcggcagcg gcatggtgga ccagtacatc cagtgcaagc acggaagggc gaaggtggag
      181 tacctccatc cccttctcga ggatgtcctg aaggaaacct acggcgtggt gctctaccag
      241 gagcaggtca tgcagtgcgc cgccattctc gcgggatact ccctgggaga ggcggatctc
      301 ctccggagag ccatgggcaa aaaaaaggcc gacgtgatgg agcagcagag ggccaaattc
      361 gtggagggtg ccaaagagcg gggcatcgac ggaaaaacgg cggagaacat tttcaacatc
      421 atccaggagt tcgccgggta cggcttcaac aagtcccaca gcgccgccta cgccctgatc
      481 acctaccaga cggcatggct gaaggccaac tacaggccgg agttcatggc cgcctacctg
      541 tcgagccaga tagggtccaa gaaagaggat cttgcggctt atgtcaggga agtaaggaac
      601 tcgggaataa aggtgcttcc tcccgacgtg aatacctcca tggccagctt cacagccgtc
      661 ggggacgtga tccgcttcgg tctcggcgct gtctccaagt caggacatac ggcggtggag
      721 gccatcctgg cggcgcggaa cgagggaggt cccttcgcgt ccctctggga ctttctctgc
      781 agggtggatc tccgggtggt gaacaagtca gttgtggaaa acctcatcag ggccggggcc
      841 ttcgacagtc tcaacgataa ccgcagacag ctcctcgagg gactgcccga tctcgtttcg
      901 gcggcttcga agaaatgcgc cgacggcaac cagtgttccc ttttcgagct gctgcccgaa
      961 gagacggatg aaggcggacc ggacctgccc gaggtggagg atttttctat ccacgaacgg
     1021 ctggagctgg aaaaagatgc cactggcctc tatatctcgg ggcacccctt cgagcagcac
     1081 gaggcgaagg tccgttcctt cagcaactgc gctattggag acctgaaaaa gtggaagggg
     1141 aaaaagaatc ctcctctcgt gggtggtatg gtccttgccg taagagagcg gacgacgaaa
     1201 aacggggatc ccatggggat aatcgaactg gaagacggag aatcccgggt ggaggcggtc
     1261 tgttttccga agacatgggc atccctcagg gggaaaattt ccgccggaca ggtctgcttc
     1321 gtctctggtt ttcccgagga acggggggaa acgagcgtca tagcaaggtc ggtgtttccc
     1381 gttgaagagg cgggcgaacg gggaagccct ccctacgtca gagtgtcggt cttcgaggat
     1441 tcgctgaaga acgtttccgt gaaggacctt ttcagggccc tcaagaccta ccccggaaaa
     1501 tctccggtgc tcttcgagat ccgcaatgac gattattcag tgaccatgct tctccaggat
     1561 gtccgggtcg atccgggggc tcccatagcc tcggtcgtcg aggaggttct tccgggagaa
     1621 atgttccaga tagggtagaa tcgtaccggg aatcgggaaa tcacccgtcg ggaacttcgg
     1681 gggagaaggg ggttttgggg aatgaccaag gtaaagattg tctgcactat cggccccgcc
     1741 tgttcgaggt acgaaaccct tgtggatatg gcgaaggcag gaatgaacgt ggcccgtttc
     1801 aatttcagcc atggagagta cgaagggcat cttgggatgc tgaatctcgt gcggacggtg
     1861 gaacgggacc tgaaggtccc catcgcgtgc cttctcgaca ccaagggacc ggaaatccgg
     1921 accgggaagg tggagggggg aacggtgagc ctcgagcagg gcagcaccct gacccttacc
     1981 accaggtcac tcttggggaa cgcttccctg gtgacggtgc actacgaagc cctgcccagg
     2041 gaagtggttc cggggcagga gattttcatc gacgacggaa ccctccattt gaaggtggag
     2101 gaaatctcgg gcacggaggt ggtctgcagg gttatcgtcg gaggccttct gggggacacg
     2161 aagggcatca acatccccgg ggcggaaata tccctcccgg ccctttcgga gaaagacgtg
     2221 gaggacatcc gctggggcct tgaacaccag atggagtata tcgccgtttc cttcgtgaag
     2281 aacaggcgtg acatcatgga agtccgcagg atcatggagg agttcggcgg ttccatgaag
     2341 gtcatcgcca agatcgaaac ccggcaggcc gtcctgaaca tcgacgagat caccgacgtg
     2401 gtggacggcg tgatgatcgc ccggggcgat ctcggcgtgg agattcccac cgaggaggtg
     2461 cccctccagc agaagcgcat catcgacatc tgccgggtga agggcaaggc ggtcatcgtt
     2521 gccacccaga tgctggactc catgatccgc aatccccgtc ctacccgggc cgaggcaagc
     2581 gatgtggcga acgccgttct tgacggcgcc gatgccgtca tgctctccgg cgagactgca
     2641 aaggg
//
LOCUS       DAOTHD010000218         2641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_46085_length_2641_cov_3.944316, whole genome shotgun sequence.
ACCESSION   DAOTHD010000218 DAOTHD010000000
VERSION     DAOTHD010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46085_length_2641_cov_3.944316"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="P5219_04765"
     CDS             <1..661
                     /locus_tag="P5219_04765"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRX26098.1"
                     /translation="TASRIAARGKEIALTFDACGGKKGSAVDRELLDFLRQERIPATL
                     FLSGRWLAKNRETARELAADPLFELANHGAEHRPLSVTGRSAYGIPGTESASGAAREV
                     EANARLLEEITGKRPRYFRSGTNHYDEVAVRVAAELGHVTVSHSHNGDGGASYSQKKI
                     ADGLLSLRGGEIVLFHMNRPEGRTAEGLKEALPLLREKGFRFVKLGERPFVLEGGTPG
                     P"
     gene            759..2174
                     /locus_tag="P5219_04770"
     CDS             759..2174
                     /locus_tag="P5219_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26099.1"
                     /translation="MDPMCFERWRDNQPLQEATEKFSAAAKNFLAVRDSAETDDRIAA
                     RKLWQNLSTQYWDVLAALVDAHTENFPEELSFDEGERLFIDFGYVSDELTPASPALRE
                     ALSPKAVPGLFQYYSFSDFIAEAYTMIMGKPVTPPRNGFSPEGKVIQMRKQLDGLQAR
                     IRIIFPVVLARQGALPSETEALLADLQRCLESYTEVSMRTRGYRESQEKEKQQMAVEH
                     HAFVEAEKRVASFLKGNGEEAAGLDGNEIQKVTGLLESAKNLARNIVFVTQEISKWER
                     RVKKTAAGMEGIPPVVRRRKLKDLLLSKKEYISLTAKSARRDPSQLCQSRKAPLSLDR
                     AAAIVEDLTALDPEMLVVARIRMYGIPRVIVVPGQGYGTYDWSDHTLLLPAFPLNNLP
                     EKAAAYALGTFRWDSDEDRIIKNSYELIKENRGKSILDLSSSFYKDYFLWLTKEKKGY
                     RILPRNTHKVFLQMFAPRSPE"
     gene            complement(2208..>2641)
                     /locus_tag="P5219_04775"
     CDS             complement(2208..>2641)
                     /locus_tag="P5219_04775"
                     /inference="COORDINATES: protein motif:HMM:NF014171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HRX26100.1"
                     /translation="DKHFRLIFHYTGSFTHLLSVSFVVILAHVTADLLRARPIYDSLL
                     LRMLRKCDGAVSVGKILLEGTVCPGSPLDGRRLREASLPEGCLLVSIHRSGEEILPNG
                     DTLLSAGDRIIALADGGRARRIREKMLDLTGVCTPEKHLWE"
BASE COUNT          562 a          802 c          818 g          459 t
ORIGIN      
        1 aacagcctca agaatcgccg ccaggggaaa ggaaattgcc ctgaccttcg acgcctgcgg
       61 gggaaagaag ggttcggcgg tggaccggga gctcctggat tttctgagac aggagcggat
      121 tccggccacc ctgttcctga gcgggcgatg gctggcgaag aacagggaaa ccgcccggga
      181 actggcggcg gatcccctgt tcgagctcgc caaccacggc gcggagcacc gtcctctctc
      241 cgtcaccgga cggtccgcct acgggattcc cgggacggaa tccgcttccg gggcagccag
      301 ggaagtggag gcgaacgccc ggctcctgga ggagatcaca ggaaaacgac cccggtattt
      361 ccgctccggc acgaaccact acgacgaagt ggccgtgagg gtggcggcgg agcttggcca
      421 cgtgacggtg agccattccc ataacgggga cggcggggca tcgtattccc aaaaaaagat
      481 tgcggacggc ctgctctccc tccggggtgg ggagatcgtc ctgttccaca tgaaccggcc
      541 tgaaggccgg actgctgaag gtctgaagga agccctgcct cttctccggg aaaagggatt
      601 ccgcttcgtg aagctcgggg agcggccctt cgttctcgaa gggggaactc caggcccgta
      661 agacggaacg gccgcttccg ggccgtatgg tatcatacag gggattccgc ctttgagcgg
      721 gagaaagggc ttttttagtg ccggacggag atgacgccat ggacccgatg tgttttgaac
      781 gatggaggga taaccagccc ctgcaggagg ccaccgaaaa gttctccgcc gcggcgaaaa
      841 atttcctggc cgtcagggat tccgccgaaa cggacgaccg catcgccgcc aggaagctgt
      901 ggcaaaatct gtcgacccag tactgggacg ttctggccgc cctggtggac gcccacacgg
      961 aaaattttcc cgaagaactt tcgttcgatg aaggggagcg gctcttcatc gatttcggct
     1021 acgtcagcga cgagctcacc cccgcctccc cggccctccg ggaagccctc tccccgaagg
     1081 ctgtcccggg gctcttccag tactactctt tttccgactt catcgcggaa gcctacacca
     1141 tgatcatggg caagcccgtc acgcccccca ggaacggctt ctcccctgag ggaaaggtca
     1201 tccagatgcg gaagcagctc gacgggctgc aggccaggat caggatcatc ttccccgtgg
     1261 tgctagccag gcagggggcc ctcccctccg agacggaagc tcttcttgcg gatcttcaaa
     1321 ggtgcctgga atcctacacc gaggtctcca tgcggaccag gggataccgg gagtcccagg
     1381 aaaaggaaaa acagcagatg gcggttgaac accacgcttt cgtcgaggcg gagaagcgcg
     1441 tcgcctcctt cctcaagggg aacggcgagg aagccgcagg gctggacggg aacgagatcc
     1501 agaaagtcac gggcctgctg gaatccgcca agaacctggc ccggaatatc gtcttcgtga
     1561 cccaggaaat ttccaaatgg gagcggcggg tgaagaaaac cgccgccggg atggagggca
     1621 ttccgcctgt cgtccgccgg aggaagctca aggatcttct cctgtcaaag aaggagtaca
     1681 tctccctcac ggcgaagtcc gcccggaggg atccgtccca gctctgccag agccggaagg
     1741 cccccctctc tctcgaccgg gcggcggcca tcgtggagga tctgacggcc cttgatccgg
     1801 aaatgctcgt ggttgcccgg atacggatgt acggcatacc acgggtgatc gtggtgcccg
     1861 gacagggata cggcacctac gactggagcg accataccct gctgctgccc gccttcccgc
     1921 tgaataacct tccggagaag gcggcggcct atgccctggg aaccttccgc tgggacagcg
     1981 acgaggacag gatcatcaag aacagctacg agctcatcaa ggagaaccgg ggcaaatcca
     2041 tcctcgacct gagttcgtcc ttctacaagg attattttct ctggctcacc aaggagaaga
     2101 agggataccg catactgccg agaaataccc acaaggtgtt tctccagatg tttgcgcccc
     2161 ggagcccgga gtgacgggaa aacgaaaaat gtaaacagct cttcacgtca ctcccagagg
     2221 tgtttctcgg gagtgcacac gcccgtaaga tccagcattt tttcccgtat gcgccgtgcc
     2281 ctgcctccgt ccgccagggc aatgatcctg tcccctgcgg agagcagcgt gtccccgttg
     2341 ggaagaatct cctccccgga ccggtggatg ctcaccagca ggcacccctc gggaagggat
     2401 gcctccctca gccttctgcc gtcgagaggc gatccgggac agaccgtccc ctcgaggagt
     2461 atcttcccca cgctgactgc accgtcacat tttctgagca tcctgagcag gagggagtcg
     2521 tatatgggcc gtgcccgaag caggtccgcc gttacgtggg caaggatgac cacgaaggag
     2581 accgacagca ggtgagtgaa agacccggtg tagtggaaga tgagccgaaa atgcttgtca
     2641 t
//
LOCUS       DAOTHD010000219         2638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_46154_length_2638_cov_3.207123, whole genome shotgun sequence.
ACCESSION   DAOTHD010000219 DAOTHD010000000
VERSION     DAOTHD010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46154_length_2638_cov_3.207123"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /gene="modA"
                     /locus_tag="P5219_04780"
     CDS             complement(<1..508)
                     /gene="modA"
                     /locus_tag="P5219_04780"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRX26101.1"
                     /translation="MKTLLRHLAVFLFFSLLFPAPSLPARAEIVAAAASLQPAMEKAI
                     PRFNSEHGTDLQGVYGASGNLARQIESGAPFGLFLSADEKWARYALEKGLLDGDPLPF
                     VKMPLVLWRMGSDAPSLDLLGSDGLKVAIANPETAPFGRMAKEYLQGKGFFEKIEKSG
                     RLILGGDVL"
     gene            complement(629..1510)
                     /locus_tag="P5219_04785"
     CDS             complement(629..1510)
                     /locus_tag="P5219_04785"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HRX26102.1"
                     /translation="MSETTAGLRKKIDSASDLQSVVRTMKSLAASSIGQYERSVAALA
                     EYFHAVEIGLGTAFRSASGSEAFSAPEGRRTEEIRAVVFGSDQGLVGEFNETVSEYAI
                     DTLKKETKRVRIWSVGERVHSQLKDAGFRMEALYPVPGSAKGIIPLVGEILLSALRGE
                     NPADGGEFYLFYNSPLSGAGYAPEGKRILPLDREWVRRITAIAWPSKSLPEILDRGNT
                     TFGGLLREYFFVSVFRACAESLASENASRLAAMERADRNIDDLLEDLGRSYHRLRQSG
                     IDEELFDVLAGYEVMSQ"
     gene            complement(1507..>2638)
                     /locus_tag="P5219_04790"
     CDS             complement(1507..>2638)
                     /locus_tag="P5219_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26103.1"
                     /translation="ILNQGRQDVLCVYCAIGQRAASVAKTVAILRDRGALGYTVVMVT
                     EGNEAPGLTYIAPYAATSIAEFFMEEGRNVLIVYDDLTNHARSYRELSLLLRRPPGRE
                     AFPGDIFYIHSRLLERATHLRKERGGGSLTALPIIATDAQNISAYIPTNLISITDGQI
                     YLSPSLFELGVLPAVDVGKSVSRVGGKAQLAAFRSVAGDLKLAYSQFEELESFARFGA
                     RLDDDARKTMAHGRRIRGSLKQPEFSPISVPAQIAILLAISAELFDEIPLNRIDEAER
                     AVQQAAEAMPDDMRERLFTADTLKEEDREAITEIARQALGTFFSGGKEEALPAEGEAG
                     EPLSEPEEPADLQLAHEAEKSGVSAAAESAKKKSPGPGKKKS"
BASE COUNT          526 a          786 c          778 g          548 t
ORIGIN      
        1 tcaatacgtc tcctccgaga atgagccttc cgcttttttc gattttctca aaaaatcctt
       61 ttccctgaag atattccttt gccatccgac cgaagggtgc cgtctcggga tttgcgatgg
      121 ccaccttcag cccgtccgag ccgagcaggt cgagggaggg ggcatcgctc cccatgcgcc
      181 agaggacaag gggcatcttt acgaaaggca ggggatcgcc gtcgagaagc cctttctcca
      241 gggcgtaccg ggcccatttt tcgtcggcgc tgagaaagag cccgaaggga gccccgcttt
      301 cgatctgccg cgccaggttt cccgacgccc catagacacc ctggagatcc gtgccgtgct
      361 cgctgttgaa ccgggggatg gccttttcca tggcaggctg gagagatgct gcggccgcga
      421 cgatttctgc ccgggcgggc agggagggag ccggaaaaag gagggaaaag aaaagaaaaa
      481 ccgcaagatg cctgagaagc gtcttcacag aaatcgtctc cttttgcaac taggttgctt
      541 tcattgtacc agagtgctga aaggatgcaa catggttgcg tttacggagc gccgttcatc
      601 tccctcatgt tcacctccgg ggattgcttt attgtgacat gacctcgtac cctgcaagaa
      661 catcgaaaag ctcctcatca attccgctct gcctcaggcg gtggtaggaa cgcccaagat
      721 cctcgagaag atcgtcgata ttacggtcag cccgctccat ggccgccagg cggctcgcat
      781 tttcgctcgc gagggattcc gcacaggcac gaaaaacgga gacgaaaaaa tattcccgga
      841 gaaggccgcc gaaggtggta ttcccccggt cgaggatctc cggaagagat ttcgacggcc
      901 aggcaatcgc ggttatccgc ctgacccatt cccgatcgag ggggaggatc cgtttccctt
      961 cgggggcata ccccgctccg gaaagagggc tattgtagaa aagataaaac tcccccccat
     1021 ctgcggggtt ctcgccccgc aaggctgaga gcaggatttc tccgacaagg gggatgatcc
     1081 ccttcgcaga cccggggacg gggtaaagag cctccattcg gaaaccggcg tccttcagct
     1141 gtgaatggac ccgctctcca acggaccaga tcctgacccg cttcgtctct tttttcagag
     1201 tgtcgatcgc atactcggag acggtttcat tgaactctcc cacaagcccc tggtccgaac
     1261 caaagaccac tgcccgtatt tcttctgttc tcctgccttc gggcgccgaa aaagcctcac
     1321 ttccggaggc gctccggaat gctgtcccga ggccgatttc cacggcatga aaatactcgg
     1381 caagggcggc cactgagcgc tcgtattggc cgatgcttga tgcggcgagg gatttcatcg
     1441 tcctgacaac ggactgaaga tccgacgcgc tgtctatctt ttttcgcagg cctgccgtcg
     1501 tctcgctcat gattttttct ttcccggtcc cgggcttttc ttcttcgcac tttcggcggc
     1561 agccgagacg ccgctcttct ccgcctcatg agcgagttgc aggtccgccg gttcctccgg
     1621 ttcagacagg ggctcaccgg cttcgccttc ggccggaagg gcttcctcct tcccgccgga
     1681 gaaaaatgtc ccgagggcct gccgggcgat ctccgtgatc gcctcccggt cctcctcctt
     1741 caacgtatcg gcggtgaaaa gccgctctct catgtcgtcc ggcatggcct cggcggcctg
     1801 ctgcacggcc cgttcagcct cgtcgattct gttcagggga atctcgtcga aaagttccgc
     1861 gctgatggcg agcaggatgg cgatctgggc cggaacagat atgggggaaa attcaggctg
     1921 tttcaaagat ccccgaatcc tccgtccgtg ggccatggtt tttcgggcat catcatcgag
     1981 ccgggctcca aaacgggcga aggactcgag ttcctcgaac tgggagtagg cgagcttcag
     2041 atcgcccgca acggaccgga aagccgcgag ctgggcctta cctccgaccc gggagaccga
     2101 tttccccaca tcaacggcgg gaagcacccc aagctcgaaa agggacgggg agaggtaaat
     2161 ctgtccgtcc gttatggaga tgaggttcgt ggggatgtag gccgatatgt tctgggcatc
     2221 cgtcgcgatg atcgggaggg cggtgagtga gccgcctccc cgctctttcc ggaggtgagt
     2281 cgcccgctca agaagacggg agtggatgta gaagatatcg ccgggaaagg cttcacggcc
     2341 gggcggacgg cgaagaagaa gggacagttc ccggtacgac cgggcgtgat tggtgagatc
     2401 gtcgtaaacg atgaggacgt tccggccctc ctccatgaaa aactcggcga tgcttgtggc
     2461 cgcgtagggg gctatatagg tgagtcccgg ggcctcgttc ccctcggtta ccatcaccac
     2521 ggtatacccg agagctcccc tgtcacggag gatggccacg gtcttggcca cggaagcggc
     2581 tctctgtccg atcgcacagt agacgcacag gacatcctgc cgtccctggt tcaaaata
//
LOCUS       DAOTHD010000220         2636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_46205_length_2636_cov_2.518016, whole genome shotgun sequence.
ACCESSION   DAOTHD010000220 DAOTHD010000000
VERSION     DAOTHD010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46205_length_2636_cov_2.518016"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="P5219_04795"
     CDS             complement(<1..652)
                     /locus_tag="P5219_04795"
                     /inference="COORDINATES: protein motif:HMM:NF014697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HRX26104.1"
                     /translation="MFRKLGQSMAAWSFKYIPDPSIFAVLLTFIAFGLGMALTPSGPM
                     DMVLHWYKGFWELLTFSMQMALMVITASTVANAPAIKKGISALASIPKNSKQAVYFVA
                     LVSVAVSIVHWGLSLVLGALLAREVGKSLKKRNMAFEYGLIGAAAYLGQMTWHGGMSA
                     SVGLLIATPGHFLEKQIGIIPIADYMFNPMNIAVTIGVLVFPPLFAWMIHPKDDEIK"
     gene            complement(866..1597)
                     /locus_tag="P5219_04800"
     CDS             complement(866..1597)
                     /locus_tag="P5219_04800"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HRX26105.1"
                     /translation="MHEIIEKLAHALLNSSSTVVFTGAGMSTESGLPDFRSPGGLWGK
                     YRPEDLASTDALNRNFEQFAAFYRQRMETLGEVRPNSGHLTLAEWERKGLVSAIVTQN
                     VDGLHQAAGSKAVHELHGTLRMVRCQSCGREFPAELFSSETACGQCGGKLRPAVVLFG
                     EMLPERTLAEATDLASSCSLFLVLGSSLAVSPANLLPERAKRHGAWLFIVNNTPTHLD
                     FIADGVLRNSIGDVLARTDDIVSKG"
     gene            complement(1650..>2636)
                     /locus_tag="P5219_04805"
     CDS             complement(1650..>2636)
                     /locus_tag="P5219_04805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRX26106.1"
                     /translation="AGMFFAALSGSSAATTAAIGVAMIDEMEKRGYPKSFAAAVVATG
                     GTVGIVIPPSVTMVVYGVIAGTSIGDLFMGGFAPGILMGGATCLVSWFIARKKGYKGE
                     GKASFARIARTFRDCIWALLMPVIILGGIYGGIFTPTEASAVAAVYGAAIGKFVYREL
                     KWKDVPKVLLSAATSTTMIMYVVGAANLFGWILTNAQVPHKIAAGFIALTDSPFIFLM
                     LVNVLLLFIGTLLNASAAVVILTPILLPVVLQLGIDPVFFGVLMVVNLAIGCITPPVG
                     LDLFVVSSITKISIDKIMRDITPYLIALLVVLFVLTWLPDIILFLPRLLGGA"
BASE COUNT          557 a          718 c          851 g          510 t
ORIGIN      
        1 gcttgatctc gtcgtccttc gggtggatca tccacgcgaa gaggggcggg aagaccaaaa
       61 caccgatggt gacggcgatg ttcatggggt tgaacatgta gtccgcgatg gggattatgc
      121 cgatctgctt ctcgaggaag tgcccggggg tggcgatgag aaggccgacg gacgccgaca
      181 tgccgccgtg ccaggtcatc tgccccaggt aggcggcggc gccgatgaga ccgtattcaa
      241 aggccatgtt tcttttcttc agggattttc cgacttcccg ggcgagaagg gcgccgagaa
      301 cgaggctgag gccccagtgg acgatggaga ccgccacgga gaccagggcg acgaagtata
      361 cagcctgctt gctgtttttc gggatggacg cgagagcgga gatgcccttc ttgatcgccg
      421 gggcgttcgc cactgttgaa gcggtgatga ccatgagggc catctgcatg ctgaaggtaa
      481 ggagttccca gaaacccttg taccagtgga gaaccatgtc catggggccg cttggggtaa
      541 gggccatacc gaggccgaac gcgatgaacg tcagaagaac cgcgaagatg cttgggtcgg
      601 ggatatactt gaaggaccat gctgccattg actgaccgag tttcctgaac atgctcatcc
      661 ctccttagat cttgtgaaat gcctgtgttt gtgcagactc tcgtcggaaa agcttcaata
      721 caggtacggg agcggaataa tccccccctt catcggatgg tggaattgtg atgacgcaca
      781 tagattgtct atgacattat tggaaaagtc aactggatgc atatgagggg tgttttttga
      841 tcaaacaagg aatgaagaga acctgttatc ctttcgaaac aatgtcgtcg gtccttgcca
      901 gaacgtcgcc gatggaattc cggagaacgc cgtcggcgat gaaatccaga tgggtggggg
      961 tgttgttcac gataaagagc catgccccgt gccgttttgc cctttcggga agaaggttgg
     1021 cgggggagac tgcaagggaa gagccgagga cgagaaagag cgaacaggag gaagcgaggt
     1081 ccgtggcttc ggccagagtt ctctcgggga gcatttcgcc gaagagcacc accgcggggc
     1141 ggagtttccc gccgcactgt ccgcaggccg tttccgagga aaacagctcc gcagggaact
     1201 ctctgccgca ggactggcac cggaccatac ggagcgttcc gtggagctcg tgcacggctt
     1261 ttgatcctgc cgcctggtgg aggccgtcca cgttctgggt gacgatagcc gacaccagcc
     1321 ccttccgttc ccattcggcc agggtgaggt gcccgctgtt cggccgcact tcgccgaggg
     1381 tttccatgcg ctgcctgtaa aaggcggcga actgctcgaa gttcctgttc agggcgtcag
     1441 tggacgccag gtcctcgggc cggtactttc cccacaatcc tcccggcgag cggaaatcgg
     1501 gcaggccgga ctcggtgctc attcccgcac cggtgaaaac caccgtggag gaggaattca
     1561 gaagtgcgtg cgcaagtttt tcaatgattt catgcatatc gggtctcctt tgaaaaaaac
     1621 cgcccggcgg accgggcggt ttttcaacgc tatgctcccc cgagaagcct cggcaggaag
     1681 aggatgatgt cggggagcca tgtgagaacg aaaagaacga caagaagggc gatgaggtag
     1741 ggtgtgatgt ccctcattat cttgtcgatg gaaatcttgg tgatgctgga caccacgaag
     1801 aggtccagtc ccaccggggg cgtgatgcac ccgatggcga ggttcaccac catgagcacc
     1861 ccgaagaaga cggggtcgat gccgagctgc agaaccaccg ggagcaggat gggcgtcagg
     1921 atgaccaccg ccgccgaggc gttgagcagc gtaccgatga agaggagcag tacgttcacc
     1981 agcatcagga agatgaacgg gctgtccgtc agggcgatga agccggccgc gatcttgtgg
     2041 ggaacctggg cgttggtgag gatccagccg aagaggttgg ccgcacccac cacgtacatg
     2101 atcatggttg tgctggtggc tgcgctcagg agcaccttgg gaacgtcctt ccacttcagc
     2161 tccctgtaga cgaacttgcc gatggccgct ccgtagaccg ccgccaccgc agaggcttcc
     2221 gtgggggtga agatgccccc gtagatgccg cccaggatga tcaccggcat gaggagggcc
     2281 cagatgcagt ccctgaaggt cctggcgatc cgggcgaagc tcgcctttcc ttcacctttg
     2341 tatccctttt tcctggcaat gaaccagctc acgaggcagg tggcccctcc catgaggatg
     2401 ccgggagcga agccgcccat gaagaggtct cctatggacg ttccggcgat gacgccgtag
     2461 accaccatgg tgacgctcgg ggggatgacg atgcccacag tgcctcccgt ggccaccacc
     2521 gccgccgcga aggacttggg atagccccgc ttttccatct cgtcgatcat ggccacgccg
     2581 atggctgccg tggtggctgc tgaagatcct gaaagggcgg cgaagaacat gcccgc
//
LOCUS       DAOTHD010000221         2629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_46362_length_2629_cov_3.737374, whole genome shotgun sequence.
ACCESSION   DAOTHD010000221 DAOTHD010000000
VERSION     DAOTHD010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46362_length_2629_cov_3.737374"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="P5219_04810"
     CDS             complement(<1..1060)
                     /locus_tag="P5219_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HRX26107.1"
                     /translation="MKRNWKTMDGNTAAAHVSYAFTEVAAIYPITPSSPMPEVVDEWA
                     AHGRKNIFGKTVQVTELQSEAGAAGAVHGSLSAGSLSTTFTASQGLLLMMPNMYKIAG
                     EMLPGVFDVTARSLAAHALSIFGDHSDVMACRGTGFAMLASGSVQEVMDLTAVSHLSA
                     IKASIPFLNFFDGFRTSHEIQKIDVLEYDDLAKIVDYDAIARFKARALNPEHPYQKGT
                     AQNPDIFFQAKEAINRFYTDIPEVVEAYMQEIKKLTGREYHPFNYYGAPDAENVIVAM
                     GSVCQAIEETVDYLNARGEKTGVVEVHLYRPFSEKFFFDALPASVKRIAVLDRCKEIG
                     ALGDPLYEDVCTLFKDKAV"
     gene            1464..2429
                     /gene="trmB"
                     /locus_tag="P5219_04815"
     CDS             1464..2429
                     /gene="trmB"
                     /locus_tag="P5219_04815"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRX26108.1"
                     /translation="MSDLKSIIVLPEKESLPLEMDRGKTGAEVFLEIGFGNGEFLENL
                     AGKNRDRVFWGVEMSRACLMRACRRFEKKELSNACLICGDARFILKECVPSCSLEGIF
                     MNFPCPWPKKKHSKRRVSSGEFPSEIARVLRPGGFFELVTDEEWYGMEVRDVLASHPA
                     LSLGAWEIDPPREVTTKYERKWLEMGKKIFLSRFVKRDGNSERKSGFLGRAEEVHVRV
                     MGKHDLDSFLDSLYGSEGEAAGGRWVFKKQYASEDGARLIEVVAADGSFEQKFFLQGI
                     QRDEDVLFKVSPYSAPFLTPSVKGAIEMLAEKLGGSPGKPLSGQG"
     gene            2445..>2629
                     /locus_tag="P5219_04820"
     CDS             2445..>2629
                     /locus_tag="P5219_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HRX26109.1"
                     /translation="MKEARPTSGKVLQALFNILGDIRGKAFLDLFSGTGRVAGFARER
                     GADPVVAVETLARRAKD"
BASE COUNT          612 a          622 c          790 g          605 t
ORIGIN      
        1 tgacagcctt gtctttgaag agcgtgcaga catcctcgta caggggatct ccgagagcgc
       61 cgatttcctt gcacctgtcg aggaccgcaa tgcgcttcac ggaggccgga agggcatcga
      121 agaagaattt ctccgagaag ggcctgtaca ggtgcacttc aacgactccc gtcttttctc
      181 ccctggcgtt caggtagtcg actgtctcct cgatcgcctg gcagacggaa cccatggcca
      241 ctatgacgtt ctcggcgtca ggtgcaccgt agtagttgaa gggatggtac tcgcggcccg
      301 tgagcttttt gatctcctgc atgtaggcct cgacgacctc ggggatatcg gtgtagaagc
      361 ggttgatggc ttccttcgcc tggaagaaga tatccgggtt ctgggcggtc cccttctggt
      421 agggatgctc cgggttcagc gcgcgggcct tgaacctggc gatggcatca taatcgacaa
      481 tcttcgcgag gtcgtcatat tcgaggacgt cgatcttctg gatctcgtgg gaggtgcgga
      541 atccgtcaaa gaagttcagg aaggggatgc tggccttgat cgctgaaaga tgggatactg
      601 ctgtgagatc catgacctcc tgcacgctgc cggaggcaag cattgcaaaa cctgttccgc
      661 ggcaggccat gacgtcgctg tggtccccga aaatggacag ggcgtgagcc gcaagggacc
      721 gtgcggtgac gtcaaagaca ccgggaagca tttcgccggc gatcttgtac atgttgggca
      781 tcatgaggag aagtccctgg gaggcagtga aggttgtgga cagagatccg gccgaaaggc
      841 ttccgtggac agctcccgct gctcccgctt cggactgcag ttcggtgacc tgaaccgtct
      901 tcccgaaaat gttcttcctg ccgtgagctg cccattcatc gaccacttcg ggcataggcg
      961 aggacggcgt tatcgggtag atcgcggcaa cttcggtaaa ggcgtatgaa acatgagccg
     1021 cagcggtgtt gccgtccatg gttttccagt ttcttttcat taaggtaccc cctcttgaat
     1081 ttcaggcagg ggcgccttaa gcccctcggc ctgattatta ttccttcgga aaagtgcgaa
     1141 aggcgaatcc ggactgccat acctttttat tttacatcaa aaaagggatt ttgcattctg
     1201 tataatctga gcgcagggaa aacaaaaaac cataaaacat acattttcac gtgctgatct
     1261 taccataaag acacgtacgg tcaatttgtg aattcacagc gaaacggagg ctgcggcatg
     1321 aggagaaaat ttcagcggcg ccgattgatt ccggtgctcc gggggaacgg ccgttttccc
     1381 ggagccgttg tgagaagccg gggacaagac tgttataatt gtagtcttga aaaccttgaa
     1441 ctgaacgaag aggggttccg aacgtgagcg atctgaaaag tatcattgtc ctgccggaga
     1501 aagaaagtct tccccttgaa atggaccggg ggaaaacagg agcagaggta tttctggaaa
     1561 tcggattcgg caatggggaa tttcttgaga accttgccgg caaaaacaga gacagggtat
     1621 tctggggcgt cgaaatgtca agggcatgcc tgatgagagc ctgccggagg tttgaaaaga
     1681 aagaactcag caatgcctgc ctcatctgcg gggacgccag gttcatattg aaagaatgcg
     1741 taccgtcctg ttcccttgag ggtattttta tgaattttcc ctgtccgtgg ccgaaaaaga
     1801 aacattcgaa gagaagggtt tcgagcgggg aatttccttc ggaaatagcc agggtgctca
     1861 gacccggagg ctttttcgaa cttgtgaccg atgaggaatg gtacggaatg gaagtgcggg
     1921 acgttcttgc atctcaccct gcgctgtcgc tgggtgcatg ggaaatcgat cctccgagag
     1981 aggttacaac gaaatatgaa agaaaatggc ttgagatggg gaagaaaatt ttcctgtcac
     2041 gttttgtgaa acgggacggg aacagcgaaa ggaaatccgg tttccttggg agggctgaag
     2101 aagtgcacgt tcgtgttatg ggaaaacatg atcttgattc tttccttgac agtctttatg
     2161 gttcagaagg cgaagcggca gggggacgct gggtttttaa aaaacagtat gcttcggaag
     2221 atggtgcccg cctgattgaa gtggtcgctg cagacggatc tttcgaacag aagttttttc
     2281 tccaggggat ccagcgtgat gaagacgtgc ttttcaaagt gtcgccctat tccgctccct
     2341 ttctgacacc atcggtcaag ggcgccattg aaatgctcgc agaaaaactc ggaggcagcc
     2401 ccgggaagcc tctcagcggg cagggctgag gaggcgcatt tcaggtgaag gaagcccgtc
     2461 cgacctcagg gaaagtgctt caggcccttt tcaatattct cggcgacatc cgcggcaagg
     2521 cgttcctcga cctgttctcg ggtaccggaa gagtcgcggg tttcgcgagg gagcgcggtg
     2581 ctgatccggt ggtggccgtt gaaaccctgg ccaggagggc aaaggacat
//
LOCUS       DAOTHD010000222         2629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_46372_length_2629_cov_3.196193, whole genome shotgun sequence.
ACCESSION   DAOTHD010000222 DAOTHD010000000
VERSION     DAOTHD010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46372_length_2629_cov_3.196193"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="P5219_04825"
     CDS             <1..1013
                     /locus_tag="P5219_04825"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HRX26110.1"
                     /translation="GLGPTHDDCTREAISSYLGVPLERADTAYDAIMARYPAEMRLPL
                     EKCRDTQGSVPRGTRAVHNPEGSALGIAFEVSGTKVYAFPGVPSEYRAMAEQELAPEL
                     APVRNGTASVLVAGLPESLLKDRLAPVISRQELHISILPSPGLVEIFMRGNPADVVQA
                     EADVRLLVPGDCLPSASRSIEEAVIQGAVHRGLKLACAESCTGGMVGAALTSVPGSSA
                     VFFGSAVCYSNSAKKSILSVNKATLNHFGAVSSQCAAEMAEGAVRVFGTDLAVSVTGI
                     AGPEGGSPEKPVGTVWFGIAGKGRTEAVKKLFSGDRNGIRNRASMFALFCLWRKVSEA
                     EE"
     gene            1010..1573
                     /gene="thpR"
                     /locus_tag="P5219_04830"
     CDS             1010..1573
                     /gene="thpR"
                     /locus_tag="P5219_04830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HRX26111.1"
                     /translation="MNGAVVRSFVCVPLPEAVKRIVGNHAAAIRREQSGLKWVEKDLY
                     HITLKFCGEHPLPVLDVFRNNAEAAINGHRPGPVLLRLGRPGAFPGMKRARTFFIGVE
                     GETDKLAGIAELIESAAEAAGMGKENRPFHPHVTLARTRRPEEIVLPFFQEKRPGEWS
                     AESILWMKSELRPSGPEYSILQEWSLL"
     gene            1625..>2629
                     /gene="recA"
                     /locus_tag="P5219_04835"
     CDS             1625..>2629
                     /gene="recA"
                     /locus_tag="P5219_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRX26112.1"
                     /translation="MAKKKTPQTREDVLEQAIEDIRGKFGEGSIMRLGESFRSAVEVI
                     PTGILPLDVALGIGGLPKGRIVEIFGPEGSGKTTVALHAVAEAQKAGGIAAFIDAEHA
                     LDPRLASTLGVDVQSLYIAQPDSGEQALYVLDTLVRSSAVDIVVVDSVAALTPQAEID
                     GKIGDSQVGLQARLMSYALRRLTSAISKSNCVVVFINQLRAKISTGYSQGPQETTTGG
                     RALKFYSSVRIEVKRGKSITKGDDTIGHELWMKVVKNKQAPPFKTGHSSLIYGKGIPR
                     GMSVVDMAIDFDVVKRKGSWIAYKGETLAQGKEALAQYLEEHPDLMKEIVDEVLSLVS
                     S"
BASE COUNT          622 a          674 c          760 g          573 t
ORIGIN      
        1 gaggtcttgg tccgactcat gatgactgta cccgtgaagc catctcatcc tatctcgggg
       61 tacctctcga aagggcggat accgcctacg acgcgataat ggcccgctat cccgcagaaa
      121 tgcggcttcc attggaaaaa tgccgggata cccagggaag tgtgcccagg ggaacccggg
      181 ctgtccacaa tcccgaggga tccgctctcg gaatcgcctt tgaagtttcc ggaacaaaag
      241 tatatgcctt tcccggtgtt ccctccgaat accgtgccat ggcggaacag gaactggccc
      301 ctgaactggc tcccgtcagg aacggaactg cttcggttct ggtcgcgggc ttgcctgaaa
      361 gcctgctgaa ggaccgcctt gcacctgtca tttccaggca ggagcttcat atttccatac
      421 ttccttcacc ggggcttgtt gaaattttta tgcggggaaa tccggctgat gtcgtccagg
      481 cggaagcgga tgtacggttg ctggtccctg gggactgtct tccttccgca tcccggtcaa
      541 ttgaagaggc ggttattcaa ggggcggtgc acaggggcct gaaactggca tgtgctgaat
      601 cctgtacagg gggaatggtg ggggctgctc ttacgtcagt tccgggaagt tcggcagttt
      661 tcttcggcag cgcagtatgc tatagtaact ccgcgaagaa aagcatactc tcagtaaata
      721 aagctacgct gaatcacttc ggtgcggtca gttcccaatg tgcggccgag atggcggaag
      781 gagcggtccg cgttttcgga accgacctgg ctgtttcggt taccggaatc gcggggccgg
      841 aaggcggctc tccggaaaaa cccgtaggaa cagtctggtt cggcattgcg gggaaaggaa
      901 ggacggaagc ggtgaaaaaa ctgttttctg gcgacaggaa cgggatcagg aacagggctt
      961 ccatgttcgc tcttttctgc ctctggagga aagtctcgga ggctgaagaa tgaatggggc
     1021 ggtggttcgc tccttcgtct gcgttcctct gcccgaagcg gtgaaacgta ttgtggggaa
     1081 ccatgcagcg gcgattcgac gggaacaatc cggcctgaaa tgggttgaga aggatctgta
     1141 tcatatcact ctgaagtttt gcggggaaca tcctcttccg gtgcttgatg tcttcaggaa
     1201 caatgcggaa gccgcaataa acgggcacag gccgggaccg gtcctacttc ggctcggccg
     1261 accgggagcc tttcccggaa tgaagcgggc acggactttt ttcatcggtg tcgaagggga
     1321 aacggacaag ctggcaggca ttgcggaact cattgaatct gcggccgagg ctgcgggtat
     1381 ggggaaagaa aaccggccct ttcaccctca tgtcacccta gcccgcacaa gacgccctga
     1441 agagattgtc ctccctttct ttcaagagaa acgacccgga gaatggagtg ccgaatccat
     1501 tctttggatg aaaagcgaac ttcggccctc cggacctgag tattccatac tgcaggaatg
     1561 gagtcttctc tgaagttcct ttccctgcag aaatttacgt ttaaaacgaa aggagtggtt
     1621 ctgtttggcg aagaagaaaa cccctcaaac acgggaagat gttctcgaac aggcaattga
     1681 agatatccgg ggcaagttcg gcgaaggttc cataatgaga cttggggaat ccttcagatc
     1741 cgctgtggaa gtcatcccca caggaatact cccccttgac gtagcccttg gaataggtgg
     1801 ccttccgaag ggaagaatcg tggaaatttt cgggcctgag ggatcgggca aaacgacagt
     1861 tgctcttcat gctgtcgctg aagctcaaaa ggccggagga atcgcggcct tcattgatgc
     1921 cgagcatgct cttgatcccc gccttgcctc caccctggga gtagatgtcc aatctctgta
     1981 tattgcccag ccggacagcg gcgaacaggc tctttacgtc cttgacaccc ttgtccgaag
     2041 cagcgcggtt gacatcgtgg ttgtggactc cgttgcggcc cttactcccc aggccgaaat
     2101 cgacggaaaa atcggagaca gccaggtggg actccaggcg aggcttatgt cctatgccct
     2161 cagaaggctc acatccgcca tttcgaagag caactgtgtc gttgtcttca taaatcagct
     2221 gagagcaaaa atcagcactg ggtacagtca gggaccccag gaaacgacca cgggaggaag
     2281 agcgctgaag ttttacagct cagtgcgtat agaagtcaaa cggggcaagt ccattacaaa
     2341 gggtgacgat accatcggcc acgaactctg gatgaaggta gtgaaaaaca agcaggctcc
     2401 ccctttcaag accggtcatt cctccctcat atatggaaaa ggaatcccca ggggcatgtc
     2461 cgttgtagat atggcaatcg actttgatgt agtaaaaaga aaaggctcgt ggatagccta
     2521 caagggtgaa accctcgccc agggcaaaga ggcgttagcc caatatctcg aggagcatcc
     2581 ggacctcatg aaggagatag tagacgaggt gctttccctt gtctcaagc
//
LOCUS       DAOTHD010000223         2627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_46433_length_2627_cov_2.644635, whole genome shotgun sequence.
ACCESSION   DAOTHD010000223 DAOTHD010000000
VERSION     DAOTHD010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46433_length_2627_cov_2.644635"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..744
                     /locus_tag="P5219_04840"
     CDS             238..744
                     /locus_tag="P5219_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRX26113.1"
                     /translation="MDLDADLVNRVTWKIPNALALVGSRSADEYNAMTTSWITQLSMS
                     PVIIGVGVDNTAVTHRLITEGGGFTVNLWDAADTKVFVKFSKPASFEDGALNGRPVRA
                     EVTGAPVFDEAIAWLDCEVRRAVDFGTHTLFAGELVAAGINDDDTRAAAMSDTRMKYG
                     GVPRGGHK"
     gene            770..1153
                     /locus_tag="P5219_04845"
     CDS             770..1153
                     /locus_tag="P5219_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HRX26114.1"
                     /translation="MTALSGRVWIRRAYDQPTRNDGYRILVDRLWPRGVSKEDLVLDE
                     WAREIAPSDDLRRYFNHDEARWESFAERYEHELTDHGNKLAELVDRTREGRITLVYGA
                     RDTEHNNAVVLGNLITHLRATRVGD"
     gene            complement(1232..1783)
                     /locus_tag="P5219_04850"
     CDS             complement(1232..1783)
                     /locus_tag="P5219_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HRX26115.1"
                     /translation="MIPFRLRRETACMVMVDVQERLLPAISGAEEIRRNTVKLAAAAK
                     VLSIPFVYTEQYPRGLGPTDGEILAALPDGVTSMEKNTFSCCDEAAFEELLQGTGRPV
                     PVIFGIETHICVLATVLDLLEKGRKVVLASDACGSRDPLKAERALSAMASAGAMVVPG
                     RSRPASGRKWLLPSCLWGSLIEP"
     regulatory      1873..1991
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            2046..>2627
                     /locus_tag="P5219_04855"
     CDS             2046..>2627
                     /locus_tag="P5219_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRX26116.1"
                     /translation="MSYLSEGFIQGLGLIFTMNEEVWTAVITTLKLSFLSMAVTLALG
                     IPGGFILGYFSFPGRRILRIMVDTLLSIPTVVVGLFVYAFISNRGPLGGYGLLFSIPG
                     MAIGQTILALPIVVALTASAVESVDDRLRLTLLTLGARGKRLALSTLWESRHQILLAA
                     VTAYGRVTAEVGVSMMIGGNIKWHTRTITTAIAL"
BASE COUNT          497 a          872 c          807 g          451 t
ORIGIN      
        1 caggatggcc agcgagacca gcacctcgat caatgtgaaa ccgccggagg atctgggtcg
       61 tccggacgtt ctcaccctta gacgctacga gcagcggcac gcacttccgc gggagcgacc
      121 tgtgacatgt gaccggacct gcgagctcga aactgcatcg gacacaccga tggggcgggt
      181 cgcgcattcc agccaacgcg acgctcacct tcggtgctcg ctggctacgg tgcggccatg
      241 gatctcgatg ccgacctggt gaaccgcgtg acctggaaga tccccaacgc gctggcgctg
      301 gtcgggtccc ggtccgctga cgagtacaac gccatgacca ccagttggat cacccagctg
      361 tcgatgtcac cggtgatcat cggggtcggg gtcgacaaca ccgccgtgac ccatcggctc
      421 atcaccgaag gcggcggttt caccgtgaac ctctgggacg cggcggacac gaaggtgttc
      481 gtgaagttct ccaaacctgc gtcgttcgag gacggtgccc tgaacggtcg tcccgtgcgg
      541 gccgaggtga cgggtgcgcc cgtgttcgac gaggcgatcg catggctgga ctgtgaggtg
      601 cgccgtgcgg tcgacttcgg gacccacacc ctcttcgccg gcgaactggt ggcagcgggg
      661 atcaacgatg acgacacgcg cgctgcagcg atgagtgaca ccaggatgaa atacggcggt
      721 gtgccccggg gtggccacaa gtgaatcggt cgcccaggag gggcagccga tgaccgccct
      781 gtccgggcgg gtctggatcc gccgggccta cgaccagccg acccggaacg acggttaccg
      841 gatactggtg gaccggctct ggccgcgagg ggtctccaag gaggatctcg tgctggatga
      901 gtgggccagg gagatcgccc ccagcgacga cctacgccgc tacttcaacc acgacgaggc
      961 gcgttgggag agcttcgcgg aacgctacga acacgaactc accgaccatg ggaacaagct
     1021 cgcagagctc gtcgacagaa cccgagaggg ccggatcacc ctcgtctacg gcgcccgcga
     1081 caccgagcac aacaacgctg tggtgctggg caatctgatc acccacctcc gggccacgcg
     1141 ggtcggcgac tgacccacct gcgggcaacg catcggagga gaagacccgg caccgggccg
     1201 ttatgagtag gggggtactc atgacagccg atcacggctc gatcagactt ccccacaggc
     1261 atgacgggag cagccacttc cggccggatg ccggtcgcga tcgccccggc accaccatcg
     1321 cccctgccga cgccatggcc gagagggccc gctcggcctt caggggatcc ctgctcccgc
     1381 aggcgtccga ggcgagcacc accttccggc ccttttccag cagatcgagc accgtggcca
     1441 aaacacagat atgggtctcg atgccgaaga tcaccggtac aggccgtccc gttccctgaa
     1501 ggagctcttc aaaggcggcc tcgtcgcagc aggaaaacgt gttcttttcc atggaggtga
     1561 caccatccgg caatgccgcc aggatttccc cgtcggtggg gccgagcccc ctggggtact
     1621 gttccgtgta cacgaaggga atggaaagca ctttcgccgc cgccgccagc ttcaccgtat
     1681 tccggcggat ttcctcagca cccgaaatgg ccggaagaag ccgctcctgg acatccacca
     1741 tcaccatgca ggcggtctcc cgccgcagtc tgaaaggaat catatattca cctccgcaga
     1801 tattctcgaa tcattgtacc ccatgagtcc cccttccggt tgccttggga aaaagagcag
     1861 ggtacaatac cttgcggaca gtccctccga gccatgaaac ctacaggcgc cagcccacgg
     1921 atccatggcc gacagggtgc tcccggaaac ggaagcgcct cccgcacggc aaggggggaa
     1981 ggacgcgaag cgtgcctttc ctgcggtatg gggaggggca ttttttattg aaagggagga
     2041 attccgtgag ctacctttcg gaaggattca ttcagggcct cggtctcatc ttcaccatga
     2101 acgaggaagt ctggacggca gtgatcacca ccctcaagct ttcgttcctg tccatggccg
     2161 tcacccttgc cctgggcata cctgggggct ttatcctggg ctacttctcc tttccggggc
     2221 gcaggatcct ccggatcatg gtggacaccc tgctctcaat ccccacggta gtggtggggc
     2281 tcttcgtcta tgccttcatt tccaaccggg gacccctcgg cggctacggc ctcctgttca
     2341 gcattcccgg catggccatc ggccagacca tactggccct tcccatcgtg gtcgccctca
     2401 cggcgtccgc tgtggagagc gtggatgacc ggctccggct caccctcctc accctgggcg
     2461 cccgtggaaa gcggcttgcc ctttccaccc tctgggagag caggcaccag atcctcctcg
     2521 ccgccgtcac cgcctacgga agggtcaccg ccgaggtggg ggtctccatg atgatcggcg
     2581 gcaacatcaa gtggcacacc cggaccatca ccacggccat cgcgctg
//
LOCUS       DAOTHD010000224         2623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_46522_length_2623_cov_2.853193, whole genome shotgun sequence.
ACCESSION   DAOTHD010000224 DAOTHD010000000
VERSION     DAOTHD010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46522_length_2623_cov_2.853193"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="P5219_04860"
     CDS             <1..1138
                     /locus_tag="P5219_04860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HRX26117.1"
                     /translation="KFGGSLKGLEAPDLGGIALKEAVKRSGVNPDDIGEVIMGNGWQA
                     GVGANPARIALFRSGLPQSIPAFTVNKRCGSSLKTAMLAADKIRLGDMKAVAGGGMES
                     ASNVPYLLKEARWGFRMGEKAAEDALHKDGFNCPLAGMLMGATAEVLNEEYSITREEQ
                     DEYALNSHRKAVAAIKEGRFASEIVPVEIRDRKKGTIVFDTDEIPREDTSLESLAKLP
                     AIFKKDGSITAGSSSALCDAGSAYIVADSDWAKANGYTPLAEIAGYASGALDAVHMGL
                     GPTVAMPKALEMAKMSLEDMEIIEINEAFSSQILACHRVMKFDMDRLNPCGGAIALGH
                     PIGATGGKILATLVYSLKAQKKELGIVSACIGGGQGVAMVIRNL"
     gene            complement(1219..>2623)
                     /locus_tag="P5219_04865"
     CDS             complement(1219..>2623)
                     /locus_tag="P5219_04865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="choline/carnitine O-acyltransferase"
                     /protein_id="HRX26118.1"
                     /translation="AAFCTEIDRGTLEPDTFRGAPLCMKEYERLFRTSRRALPGTDRQ
                     VTGTKSGPAARCALVISCGIPFLLELYSEEGGLRDVEETARHLRDIASLPPGEIPPVG
                     LITTLQRDEAARGRRLLLDGGKDNERLLSLAEESLFVLRLDGPCGSSPEERARHFLFD
                     GGQNGWYEKSFQLIVTTDGQAGINFEHSSRDGTHVGRLVKEILSRAPSVTGRATGLRG
                     PVRLDFALSGECRDFLDGIPEKARALSDSRVQDVFRFDAFGTDAVKEGNVSPDAFVQI
                     AMLMAAENLWGERRSVYESVQLRRFAGGRTEGTRPLTREAAACVDGFRSGRESSGALR
                     RMLFEAQRAHRERIRECLEGRGVEGHLQLLRGLWKERGRELGLKEEPALFRSPAWEKL
                     TSCPVSSSTTPGEGFALAGYGPVQPGGLGVRYLSRRDHFIFHVSSWKRDGSLAPEYAS
                     RLRNALEAMGGVLQDRE"
BASE COUNT          513 a          857 c          820 g          433 t
ORIGIN      
        1 caagttcgga gggagcctca aagggcttga ggctccggac ctcggaggca tagcactgaa
       61 ggaggcggtg aagcggtcgg gcgtaaaccc cgacgatatc ggtgaggtca tcatgggcaa
      121 cggatggcag gcaggcgtcg gggccaaccc cgcacggatc gccctgttcc gttcgggtct
      181 tccccagtcg atccccgcgt ttaccgtgaa caagcggtgc gggtccagcc tgaagacggc
      241 catgctggca gcggacaaaa tccgtctcgg cgacatgaaa gccgtcgccg gcggcggtat
      301 ggagagcgcc tcgaacgtgc cctacctgct caaggaagcc cggtggggat tccgcatggg
      361 cgaaaaagcg gccgaggacg ccctccacaa ggacggcttc aactgccccc tggcgggcat
      421 gcttatgggt gccacggcgg aagtcctcaa cgaggagtat tccatcacac gggaagagca
      481 ggacgagtat gccctgaaca gccacaggaa ggccgtggcc gccataaaag aaggccgctt
      541 cgcctccgag atcgtcccgg tggaaatacg ggacaggaaa aagggaacca tcgtcttcga
      601 cacagacgag attccccggg aggacacctc tctcgagtcc ctcgccaaac tcccggcgat
      661 cttcaaaaag gacggctcca tcaccgcggg gtccagctcg gccctctgcg acgccggaag
      721 cgcatacatc gtggcggaca gcgactgggc aaaggccaac ggctatactc ccctggcgga
      781 gatcgccggg tacgccagcg gggccctcga cgccgtccac atggggctcg gccccacggt
      841 ggccatgccg aaggccctgg agatggcaaa aatgtccctg gaggacatgg agatcatcga
      901 gatcaacgaa gcgttctcgt cacaaattct cgcatgccac agggtgatga agttcgacat
      961 ggacaggctg aacccctgcg gaggggccat cgccctcggc caccccatcg gggcaacggg
     1021 cggcaagatc ctcgccaccc tggtctactc cctcaaggcg caaaaaaagg aattgggcat
     1081 cgtcagcgcc tgcatcggcg ggggccaggg cgtggcaatg gtgatccgga acctgtagcg
     1141 tcggcacagg cggcgcatac atgacagcag ggagtccggc ttcttcttcc ggactccctg
     1201 ctgctttcgg cggcttcgtt attcccggtc ctgcaggacg cctcccatgg cttcaagggc
     1261 attccgcagc cgggaagcgt attccggcgc cagggagccg tcccgtttcc atgatgacac
     1321 gtggaagatg aagtgatccc tgcgggagag atagcggaca cccagccctc ccggctggac
     1381 ggggccgtat cccgcgaggg cgaatccctc tcccggcgtc gtgctgctgg acaccgggca
     1441 ggacgtcagt ttttcccagg cgggggaacg gaaaagggca ggctcctcct tcaggccaag
     1501 ctcccttccc ctctctttcc acaggcccct cagaagctgg agatgtccct ccacgccccg
     1561 tccttccagg cactcccgta tcctttcccg gtgggcccgc tgggcttcga acagcatcct
     1621 ccggagagcg ccggaagatt cccggcccga acggaagccg tcgacacaag cggccgcttc
     1681 ccgggtgagc ggacgggttc cctccgtgcg tccccctgcg aagcggcgga gctggaccga
     1741 ctcgtagacg ctcctgcgct ccccccacag gttctccgcc gccatgagca tggcaatctg
     1801 gacaaaggcg tcagggctga cgttgccttc cttcactgca tcggtcccga aggcgtcaaa
     1861 cctgaaaacg tcctgaaccc tggaatcgga aagagccctg gccttttccg gaatcccgtc
     1921 gagaaaatcc ctgcattccc cggagagggc gaagtccagc cggaccggcc cgcgaagccc
     1981 cgtcgctctt cccgtcactg aaggcgcccg ggaaaggatc tccttcacga gccgccccac
     2041 gtgggtcccg tccctggagg agtgttcgaa gttgatcccg gcctgaccgt ccgtcgtgac
     2101 gatcagctgg aaggattttt cataccagcc gttctgccct ccgtcgaaga gaaagtgacg
     2161 tgcccgctcc tcggggctgg agccgcacgg accgtcgagg cggaggacaa aaagggattc
     2221 ctcagcgagg gaaaggagcc gctcgttgtc ttttcccccg tcaaggagaa gccgccgtcc
     2281 ccgggccgct tcatcccgct gaagggtggt gatcagcccc acgggaggga tttctcccgg
     2341 agggagggac gcgatatccc ggagatgacg ggccgtctcc tccacatccc tcaggccccc
     2401 ctcctccgag tacagttcga ggagaaaggg aattccacag gagatgacga gggcacagcg
     2461 cgcggcagga ccggatttcg ttccggtcac ctgcctgtcg gttccaggca gagccctccg
     2521 ggaagtccgg aagagccgtt catattcctt catgcacagg ggtgctcccc tgaacgtgtc
     2581 cggttctagg gtccccctgt cgatctcggt gcagaacgcc gcg
//
LOCUS       DAOTHD010000225         2620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_46604_length_2620_cov_2.397271, whole genome shotgun sequence.
ACCESSION   DAOTHD010000225 DAOTHD010000000
VERSION     DAOTHD010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46604_length_2620_cov_2.397271"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..2360
                     /gene="pbpC"
                     /locus_tag="P5219_04870"
     CDS             108..2360
                     /gene="pbpC"
                     /locus_tag="P5219_04870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HRX26119.1"
                     /translation="MRGSFRRLLPAAVVSALLAGGAFLFLASLPYPENAITGWPTSPV
                     LLDRKGGVFAVFLSENSEWSIPVPLGAMGKWLPLVAVEVEDRRFREHIGIDWIALLRA
                     TIQNARAGRVVSGASTITSQLVRLTEPRPRTLKTKILEFTAAVKTEQHFSKDRILELY
                     LNRAPFGGNIRGVEAAARIYFSKSATEVSLAEAALLVGMLRGPSVYRPDRNPEAAIGR
                     RNAILSSLAGRGVITAEQYSHALAEKLPQSRGNLPAKAWHFALAALGDRKEGGTIRTT
                     LDSSVQALLERTLASALSSMPPEVTAAGIVMDNDTGDILAYSGNGRLGSGLPGSWVDC
                     ARSPRSPGSALKPFAYLAAFDRGILTPSSLLADTPLAFSGQAPRNFDLTYRGPVTART
                     ALADSLNVPAVRVLRAAGPELVLDLLRNAGFLTLNRPTGHYGDSLILGGCEVTLLQMA
                     EGYGVLAALGVHRRPRFLSGEPFPERRILPEAAPFLVADILKDTGRLLPVHGRRIEHR
                     RDWFAFKTGTSYGYRDAWTAAYNPRHTVVVWMGDPAGAPHPELVGLSAAAPAIVEVLR
                     ALPATVWYEPPPGVESRTVCTLSGAPPSPLCPSTRTEYAIAGVSSTTPCTLHVIRNGA
                     PAVRWPAELEEFALRRSLEFETGRTVSIVSPLPGSRYFLTPLGGEQKTALKAEGAVPP
                     VYWFVGGAFAGRQEGNAPLFWSLRKGRHTVSLVDSRGRTASSWVIVEAIEKGPEHRPG
                     PSSLLLTPVD"
     gene            complement(2344..>2620)
                     /locus_tag="P5219_04875"
     CDS             complement(2344..>2620)
                     /locus_tag="P5219_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HRX26120.1"
                     /translation="ATVLKVSPWEAALGASLPVDTPFGSVTMKLPGGTQAGRKLRLKG
                     KGLPRRKGALPGDLLVRVEMVIPEKLTDRERELLETLARESSFNPRG"
BASE COUNT          447 a          922 c          781 g          470 t
ORIGIN      
        1 cgtcagcagg ggaaccttca ccctgccgcc cctggccgcg gagggaatgt acgctcccga
       61 caccggcacc gtgaccgccg caggcacggt ggaaatccgc tgacgccatg agaggctcct
      121 tccgccgcct cctgccggcc gcagtagttt cggcccttct ggcgggagga gcctttctct
      181 tcctcgcatc ccttccctac ccggagaacg cgatcaccgg ctggccgacc tcgcccgtgc
      241 tgcttgacag aaagggcggg gtcttcgccg tttttctctc ggagaattcg gaatggtcaa
      301 tacccgttcc cctcggcgcc atggggaaat ggctgcccct cgtggccgtg gaggtggagg
      361 accgccgttt ccgggaacac atcgggattg actggatcgc ccttctccgg gctaccatcc
      421 agaatgcccg ggccggccgg gtggtctcgg gcgcttccac catcaccagt cagctcgtac
      481 gccttactga accccggcca aggaccctga aaacgaaaat tctcgagttc actgccgcag
      541 tgaagacaga acagcatttt tccaaggacc ggattctcga gctctacctg aaccgggccc
      601 ccttcggggg caacatccgg ggggtggagg cagcggccag gatctatttt tccaagtctg
      661 ccacggaagt ctcccttgcc gaggccgccc tgctcgtggg catgctcagg ggcccctcgg
      721 tctacaggcc tgaccgcaat ccggaagcgg ccatcggccg caggaacgcc atcctctctt
      781 cccttgccgg caggggggtg atcacggctg aacagtacag ccacgccctg gcggaaaagc
      841 tcccccagtc gagaggaaac cttccggcga aagcatggca ttttgccctc gccgccctcg
      901 gtgacaggaa ggagggcgga acgatccgga ccaccctgga ttcctccgtg caggctctcc
      961 tggagagaac cctcgcctcc gccctgtctt ccatgccgcc ggaagtgacc gcagccggaa
     1021 tcgtaatgga caacgacacg ggcgacatcc tcgcctactc ggggaacgga aggcttggaa
     1081 gcggcctgcc gggaagctgg gtggactgcg caaggagccc caggtccccg gggtcggctc
     1141 tcaagccctt cgcctacctc gccgccttcg accggggcat actcaccccg tcaagtctcc
     1201 tggcggacac tcccctcgcc ttttccggcc aggccccgag aaatttcgac ctgacctacc
     1261 gggggcccgt cactgcccgg accgccctgg ccgattccct caacgttccc gccgtaaggg
     1321 tcctccgggc ggcgggtccg gaactggtgc tcgacctgct gcggaatgcg ggcttcctga
     1381 cactgaaccg ccccaccggc cattacggag actccctcat cctcggaggc tgcgaggtca
     1441 ctctcctcca gatggcggaa ggctacggtg tcctcgccgc tctcggcgta caccgacggc
     1501 cccgcttcct gtccggggag cctttccccg aacggagaat cctccctgag gccgctccct
     1561 ttctcgtggc ggacatcctg aaggacacgg gacggcttct cccggtccac ggaaggcgta
     1621 tagaacaccg gagggactgg ttcgccttca agacgggcac gtcctacggg taccgggacg
     1681 cctggacggc ggcctacaat ccccggcata cggtggtggt ctggatgggc gacccggcgg
     1741 gggcacctca ccctgaactg gtcgggctct ccgccgcggc tccggccatc gtggaagtgc
     1801 tcagggctct tcccgcgact gtatggtacg aaccacctcc cggagtggaa tcccggacag
     1861 tctgcaccct gtccggagcc cctccctctc ccctgtgccc ctcaacccgg accgagtacg
     1921 ccattgcggg cgtttcgtcc accacgccct gcaccctgca cgtgatccgg aacggcgcgc
     1981 cggcggtgcg gtggcctgcg gagctcgagg aattcgcgct ccgccgttct ctggaattcg
     2041 aaaccggaag aaccgtatcc atcgtctccc ctcttccggg atcccgctat ttcctcactc
     2101 ctctcggagg ggagcagaaa acggccctga aagcggaggg agctgtcccg ccggtctact
     2161 ggttcgtggg aggcgccttc gccggaagac aggaaggaaa cgctcccctg ttctggagcc
     2221 tcagaaaggg ccgccacacc gtatccctcg tggattcacg gggcaggacg gcatcatcct
     2281 gggtaatcgt cgaagccata gaaaaagggc cggagcatcg ccccggccct tcgtcccttc
     2341 tgcttacccc cgtggattga aggaggattc cctcgccagg gtttccagca gttccctctc
     2401 cctgtccgtc agtttctcag gaatcaccat ctccacccgg acgagaaggt ccccgggaag
     2461 ggcgcctttc cttctcggaa gacctttccc cttgagtcgg agttttctcc cggcctgggt
     2521 tccgccggga agcttcatgg taaccgaacc gaaaggggta tccacgggaa ggctcgcacc
     2581 cagggccgcc tcccagggag acaccttcag aacagtggcg
//
LOCUS       DAOTHD010000226         2619 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_46624_length_2619_cov_3.204368, whole genome shotgun sequence.
ACCESSION   DAOTHD010000226 DAOTHD010000000
VERSION     DAOTHD010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46624_length_2619_cov_3.204368"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="P5219_04880"
     CDS             complement(<1..901)
                     /locus_tag="P5219_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26121.1"
                     /translation="MAVVEMMGMSLIGPRQEIEALAAELLTLGNFEPVPLEFALERRP
                     ASLSSGGARISTFQKNPYDDLLDKLVYVWNEAGAEFPAERACSRGTPSLSWEECSRQV
                     LDVAKKIDLWKKRAEVLEEEREKLMAAGAFLDALASADRDVEETLKTRYLSITFGRLS
                     AENYKKLEEMAAVTPLLVYPVVAEKGYVLAIVFCSRDYQEEAEKIFRTVYLKEYALGD
                     LFEGAGTNLRERLRERQLDSERAIRALRDAPRNYLGRRRKELERLYCAVHSLQRVYAL
                     CQKRGEMSGIFVLSGVIPAPTLGE"
     gene            complement(892..2016)
                     /locus_tag="P5219_04885"
     CDS             complement(892..2016)
                     /locus_tag="P5219_04885"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HRX26122.1"
                     /translation="MSFLAAGERAAVTAKARVYRGRLLAPSDYLRLLEFETVGEIAAY
                     LAKTEAYAPYITEPSPETLHRGDLEAIINSVPLLEEIPFCRYLGPERCALLRAWGERF
                     DVDVIKRVLRMITTGVGNRETLRRRVASVPITVADGEKLLAAESLREVLESLRGYPLY
                     DILAEPLKKIAKEGGTLFRPKMAMDAFFLTRILSGGKKLPGSEGRGVRQIFGTRADLI
                     NMYWIYRSRRFFSLTPEEALGLTLPVRYRLKFDTLSSFAFAPDVPSMVKLLRTGIYGE
                     AFRTSGGNEVPEVGEMTLEHNLYRILWKTASAIFRSGSAGVHVVFAYLTLRELEVKDL
                     FTIIEDVRYHYDRKKAREFLIHPAAASFAGREREVEPSWL"
     gene            complement(2017..2355)
                     /locus_tag="P5219_04890"
     CDS             complement(2017..2355)
                     /locus_tag="P5219_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HRX26123.1"
                     /translation="MNLSEVLTALLAAEDEAADSVEDARRKAADILRENREKFAADQE
                     ARLTAARAQAKAIVESARHSAELEAAQIADMGMRGRQKMKDQFAEKAPQVVSQFAEEI
                     AGRYGKKGGA"
     gene            complement(2352..>2619)
                     /locus_tag="P5219_04895"
     CDS             complement(2352..>2619)
                     /locus_tag="P5219_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HRX26124.1"
                     /translation="TMDGAYLHATEVLSLIARLAEVENSVYRLAVQIRRTFRRVNALE
                     KVVIPSHRQMLAWIANVLEENDREDFTRMKMAREGMENEGGGIL"
BASE COUNT          446 a          844 c          787 g          542 t
ORIGIN      
        1 cttcccccag tgtcggggcg gggatgactc cagacagaac gaagataccg ctcatctcac
       61 cccgcttctg gcagagggcg taaactctct ggagggagtg gacggcgcag tagagccgct
      121 ccagttcctt ccttcttctt cccaggtagt tccggggagc atcccggaga gcccggatgg
      181 cccgctcgga gtcgagctgc cgttccctga gccgttcccg aaggttcgtc cccgcaccct
      241 cgaagaggtc gccgagtgcg tattccttca ggtagaccgt gcggaagatt ttttccgcct
      301 cctcctggta gtcccgggag cagaagacga tggccaggac ataccctttt tccgccacca
      361 cggggtagac cagcaggggc gtgacggcgg ccatttcctc gagcttttta tagttttcag
      421 cgctcaggcg accgaaggtg atggaaaggt accgggtctt cagtgtttct tccacgtccc
      481 ggtcggcgga cgccagggcg tcgaggaagg cccccgccgc catgagcttc tcccgctctt
      541 cttccaggac ttcggcccgc ttcttccaga ggtcgatttt ttttgccacg tcgagcacct
      601 gccgggagca ctcttcccag gagagggagg gggtgccgcg gctgcaggcc cgttcggcgg
      661 gaaattcggc cccggcctca ttccagacgt agaccagctt gtccaggaga tcatcgtagg
      721 ggttcttctg gaaggtggaa atccgggccc ctcctgaaga cagggacgct gggcgccgtt
      781 ccagggcgaa ttccagggga acgggctcga agtttcccag ggtgagcagt tccgcagcca
      841 gtgcctcgat ctcctgcctg ggtccgatga gggacatccc catcatttcc actacagcca
      901 cgacggttcg acctcccttt ccctgccggc gaaggatgcg gcggcgggat ggatgaggaa
      961 ttcccgggcc ttcttcctgt cgtagtggta ccgcacgtct tcaataatgg tgaacaggtc
     1021 cttcacctcc agttctctca gtgtaaggta cgcgaacaca acatgaactc cggcggagcc
     1081 ggagcggaag atggccgacg ccgtcttcca gaggatccgg tacaggttgt gctccagggt
     1141 catctctccc acctccggaa cctcgtttcc gccggaggtg cgaaaagcct cgccatagat
     1201 gcccgtccgg agcagcttta ccatggacgg cacgtccgga gcgaaggcga aggagctcaa
     1261 cgtgtcaaat ttcagcctgt accgcaccgg cagcgtgagg ccgagagcct cctccggcgt
     1321 gagggagaaa aaccggcggc tccggtatat ccagtacatg ttgatcaggt ccgcccgtgt
     1381 gccgaaaatc tgccggacgc cccgtccttc gctccccggg agcttttttc ccccggagag
     1441 aatcctggtg agaaagaagg catccatggc catcttcggc cggaagaggg tgcctccttc
     1501 cttcgcgatc tttttcaggg gttcggcgag gatgtcgtac agggggtatc cccgaaggga
     1561 ctcgaggacc tcccgcaaac tttcggccgc caggagcttt tcaccgtccg ccacggtgat
     1621 ggggacggac gccaccctcc gccggagggt ttccctgttt cccactcccg tggtgatcat
     1681 ccggagtacc cgcttgatca cgtccacgtc gaaccgttcg ccccaggccc ggagcagggc
     1741 gcatcgttcc ggccccaggt agcggcagaa ggggatttcc tccagaagag gaacggagtt
     1801 aatgattgcc tccaggtctc cccggtgcag ggtctccggc gacggttccg tgatgtatgg
     1861 ggcgtaggcc tcagtctttg cgagataggc tgcaatttca cccaccgtct cgaattcgag
     1921 aaggcgaagg taatcggatg gtgcgagaag ccttccccgg tagaccctgg ccttcgccgt
     1981 caccgcggcc cgttcccccg cggcgagaaa ggacacctag gcccctcctt tttttccgta
     2041 ccgcccggcg atctcttcag cgaactggga gaccacctgg ggcgcttttt ccgcaaactg
     2101 gtccttcatc ttctgccggc cccgcatccc catgtcggcg atctgggccg cctcgagttc
     2161 agcggaatgg cgggccgatt ccacgatggc cttcgcctgg gccctggcgg cggtgagccg
     2221 ggcttcctgg tccgctgcga atttctccct gttttcccgg aggatgtccg ctgccttccg
     2281 ccgggcatcc tccacggaat ccgccgcttc gtcctccgca gccagaaggg cggtcagcac
     2341 ttcgctcagg ttcataggat ccctcctcct tcgttttcca ttccctcgcg ggccatcttc
     2401 atcctggtga aatcctcccg gtcgttctcc tcgagcacgt tggcgatcca cgcgagcatc
     2461 tgccggtggg acggaatgac caccttctcc agggcgttga cccgccgaaa ggtcctccgg
     2521 atctggaccg caagacgata gacggagttt tccacctccg ccagcctggc gatgagggag
     2581 agcacttccg tggcgtgaag gtaggctccg tccatggtt
//
LOCUS       DAOTHD010000227         2609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_46859_length_2609_cov_2.470243, whole genome shotgun sequence.
ACCESSION   DAOTHD010000227 DAOTHD010000000
VERSION     DAOTHD010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46859_length_2609_cov_2.470243"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1318
                     /locus_tag="P5219_04900"
     CDS             <1..1318
                     /locus_tag="P5219_04900"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRX26125.1"
                     /translation="GGTWRRKEEDGMLLLVGVFLVLLFLGMPVAFAIGLAGFAFMLAT
                     PNIPISISVQRIVAQTQSFTLLAIPLFIFAGNLMNATGITARLVKLSRVLVGHMAGSL
                     AQVSVILSTLMGGVSGSANADAVMECRILGPEMIKQGYSRGYGAAVNGLTAMITCTIP
                     PSMGFIIYGSVGEVSIGRLFVGGIIPGLLMMAMMMFTVNFTAKRRGYVARKDVRKPNA
                     REVVSALWENIFALIFPVILIVGIRFGLFTPSEAGAFAAGYAIFVGVFIYREMTWKSF
                     LEAVEQSAVDIGVLMLILGLSGTFGYAIVYGRVPQTIAEFLIGITSNSHLLLFLIILL
                     LILAGMFVETTVCAMLLTPVFIPVISRLGIDPVHFGVIMMTTLTAGIMTPPVGVALYS
                     TSEIMGCTPQETAREAIPFYLTLLALVIVLVLFPRIVLVLPDLVFG"
     gene            1507..2388
                     /locus_tag="P5219_04905"
     CDS             1507..2388
                     /locus_tag="P5219_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HRX26126.1"
                     /translation="MKKKLQAVLAALAILAGAFPATAKTTVFVSVLPQKFFAEQVGGE
                     RVEISVLVEKNRDPHTFEPLPAQMAALSLADGYLSIGLPFEESLLPRVRQLNPSLRIF
                     PADLGIEKIRGKDEEHHGHTHEHGHAHNHGGNDPHVWNGIREARTIAENTCTALAELD
                     PEGAEYFGRNLGNFLKRLDALDGEFRELFRGREGAAFLVFHPAWGYLAREYGLEEVAI
                     EVDGKEPKAADMAAVITEAKRRGVKVVFVSPQFSGRSAEIIARSIGAAVEAVNPLSEE
                     WEENLRTVARAIAEAAR"
BASE COUNT          458 a          819 c          798 g          534 t
ORIGIN      
        1 gggcgggacg tggcgtagga aggaggagga cggtatgctt cttctcgtcg gcgttttcct
       61 ggtgctcctc ttcctcggca tgcccgtggc cttcgccatc gggctggcgg gcttcgcctt
      121 catgctggcc acgcccaaca tccccatttc catctcggtg cagcgcatcg tggcccagac
      181 ccagagcttc accctcctgg ccatccctct cttcatcttc gccggaaacc tcatgaacgc
      241 cacgggcatc accgcccggc tggtgaagct ctcccgggtg ctcgtggggc acatggcggg
      301 cagcctcgcc caggtcagcg tcatcctgag caccctcatg gggggcgtgt ccggatcggc
      361 caacgccgac gccgtcatgg agtgcagaat cctcggcccg gagatgatca aacaggggta
      421 ttcccggggc tacggcgcgg cggtcaacgg cctgaccgcc atgatcacct gcactatccc
      481 gccgagcatg ggcttcatca tctacggctc cgtgggagag gtgtccatcg gccgcctctt
      541 tgtgggaggc atcatccccg gactcctcat gatggccatg atgatgttca ccgtgaactt
      601 cacggccaag cggcggggct acgtggcccg gaaggacgta cggaagccga atgcccggga
      661 ggtggtgtcc gccctgtggg agaacatctt tgccctgatc ttccccgtca tcctcatcgt
      721 gggaatccgg ttcggcctct tcacaccctc ggaggcgggg gccttcgccg cggggtacgc
      781 cattttcgtc ggggtcttca tctaccggga gatgacctgg aagagcttcc tggaggcggt
      841 ggagcagagc gccgtggaca tcggcgtcct catgctcatc ctcggcctgt cgggaacctt
      901 cgggtatgcc atcgtctacg ggcgggttcc ccagaccatc gcagaattcc tcatcggcat
      961 cacgtcgaac agccatcttc tcctcttcct gatcatcctg ctgctgattc tggccggaat
     1021 gttcgtggag accactgtct gcgccatgct cctgacgccg gtgttcattc cggtgatttc
     1081 gaggctcggc atcgaccccg tccatttcgg ggtcatcatg atgaccaccc tgaccgcggg
     1141 aatcatgacg ccgccggtgg gcgtggccct gtactccacg tcggagatca tgggatgcac
     1201 accccaggag accgccaggg aggccattcc cttttacctt accctccttg ccctggtgat
     1261 cgtcctcgtt ctcttccccc ggatcgtcct ggtcctgccc gacctggtct tcggatagac
     1321 ggacggaaca ggcgcggcgc tgttcggaat tgcgggggga ctcttcagcg ggtctccccg
     1381 cttttttcct gccctgagag ccccttccgg acaccttccg tttttccgct tcctgaggtt
     1441 atactattct cactccccgg aaggggagaa aggaaacggc cggcgtgccg ttccggggag
     1501 gtcatcatga aaaaaaagct gcaggccgta ctcgcggccc tggccattct tgccggagcg
     1561 tttccggcga cggcgaaaac aaccgtgttc gtctccgtgc ttccccagaa attctttgct
     1621 gaacaggtcg gcggggaacg ggtcgaaata tccgttctcg tggaaaaaaa ccgggatccc
     1681 cacaccttcg agccccttcc ggcccagatg gcagccctct ccctggctga cgggtacctg
     1741 tccatcggcc tgcccttcga ggaatccctc ctgccgaggg tcagacagct caacccgtcc
     1801 ctcaggatct ttccggcgga cctgggaatt gagaagatca ggggaaagga tgaagagcat
     1861 catggccata cccatgaaca cggccatgcc cacaatcacg gcggaaacga tccccacgtg
     1921 tggaacggaa tcagggaggc ccggacaata gcggaaaaca cctgcaccgc cctggcggaa
     1981 ctggacccgg agggagcgga atacttcggc cgcaatctcg gaaatttcct gaaacggctt
     2041 gacgccctgg acggggagtt ccgggagctc ttccggggca gagaaggggc ggcctttctt
     2101 gtctttcacc cggcctgggg gtaccttgcc agggagtacg gcctggaaga ggtggccata
     2161 gaggttgacg gaaaggagcc gaaggctgcc gatatggccg cggtgatcac tgaggcgaag
     2221 cggcggggag tgaaggtggt cttcgtctcg ccccagttct cgggaagaag cgcggaaatc
     2281 atcgcccgga gcatcggcgc ggcggtggag gcggtaaacc ccctgtcgga ggaatgggag
     2341 gaaaacctca ggacagttgc ccgggccatc gccgaggcgg cgcggtagtt cgtcttgacc
     2401 ccggagcttc ctttcggtat ggtgttttct gtcgaaccgc acatttcagg aggattatga
     2461 tggaaatgga aaacattctg ttcgctttcg gactgaccct cttcgcgggg ctgtccacgg
     2521 gggtgggaag cgccctggcc ttcttcacaa agaagaccaa cacccgcttc ctcgccttct
     2581 ccctcggtct ctcggcaggg gtcatgatg
//
LOCUS       DAOTHD010000228         2607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_46915_length_2607_cov_2.862069, whole genome shotgun sequence.
ACCESSION   DAOTHD010000228 DAOTHD010000000
VERSION     DAOTHD010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46915_length_2607_cov_2.862069"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..675
                     /locus_tag="P5219_04910"
     CDS             94..675
                     /locus_tag="P5219_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRX26127.1"
                     /translation="MAYFLARNTFRGKSVLEAIIDVPIMIPHTVAGIAVLMAFSPKAP
                     AGALLTKAGLSPVNSHLGIILACMFVSIPFYVDAARDAFYGVSPRIERAARTLGAPFL
                     SVFFRVSLPLARKGLISGLIMSWARSVSEFGAVVIIAYHPMVAPTLIYDRFATFGLKY
                     STPVAVQLILVCLGMFVVLRLLAAGKRREDEER"
     gene            672..1700
                     /locus_tag="P5219_04915"
     CDS             672..1700
                     /locus_tag="P5219_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX26128.1"
                     /translation="MIALEGLSMDLGGFRLESLSLRVAPGEFFMIVGPSGAGKTLLLE
                     AVAGLRPLSSGRILIAGKDVTAVPPEKRGVGIVYQDYALFPHLTVEENIRWGLRFSRK
                     DGSGSVDRLVDLLRLRPLLRRSPGTLSGGEQQRTALARALAVQPSVLLLDEPLSALDP
                     AFREELRDYLAEINREGMTLVMVTHDFGEVLSLGDRVAVVSEGSLQQTGNVRTVFREP
                     ANRTVAEFVGMKNIFQCEVRHGKAFLGNGAVLEVEESSPAGTRLAGIRPEDVALVRDV
                     PDGTANAFPGRIASLVPRGMAFEVIVETEAGIRLSASVLSSVVAAGGVPVGGRCVAAA
                     APDSVHVF"
     gene            1732..>2607
                     /locus_tag="P5219_04920"
     CDS             1732..>2607
                     /locus_tag="P5219_04920"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRX26129.1"
                     /translation="MKRFRFSSVLLLALLLTFSPGCFAAEKVTVYHAGSLAAPMAKIE
                     ALYEAAHPDIDILRESGGSAALARKITDLGGGCDVFLSADYMVIEKLLRPGAADWNAL
                     FASNAIVLMYGPKSKYGDEIGPENWTGVMLRPDVRWGHSEPDADPCGYRSLMVLQLAE
                     KFYGKQGLYEQAMKHQGRAVRQKAIELVAMVESGTMDYAFEYKSVAVQHGLKYLELPV
                     EINLMEPALADAYSAASVELAGKEPGKKMTVKGEPIVYGLTIPKGAPNAGAALELVKF
                     ILDPEGGLAVFRDMGQ"
BASE COUNT          437 a          815 c          844 g          511 t
ORIGIN      
        1 tccttttcgg acagcgaaat tgtcggcgcc ctctggagga gcatctgaac cgccgcggcg
       61 gcaacgggcg tcattgccct cttcggaacg cccctggcct atttcctcgc ccggaacacc
      121 ttccggggaa agtccgtcct ggaggcgatc atcgacgttc ccatcatgat tccccacacc
      181 gtggccggca tcgccgtcct gatggccttc tccccgaagg cccccgccgg tgccctgctg
      241 accaaggccg ggctttcccc ggtgaacagc cacctcggca tcattctcgc ctgcatgttc
      301 gtttccattc ccttttatgt tgacgccgcc cgggacgcgt tttacggcgt ctcgccgaga
      361 atagaacggg cggcccggac cctgggagcg ccgtttctct ccgtgttttt ccgggtttcc
      421 cttcccctgg ccagaaaggg gctcatctcc gggctgataa tgagctgggc ccggtcggtc
      481 agcgagttcg gggcagtcgt gatcatcgcc taccatccca tggttgcccc gaccctcatc
      541 tatgaccgtt tcgccacctt cgggctgaaa tactccaccc cggtcgcagt ccagcttatc
      601 ctggtctgcc tcggcatgtt cgtggtgctg aggctcctgg ccgccgggaa gcggcgggag
      661 gacgaggagc gatgatcgcg ctggaaggcc tttccatgga cctgggcgga ttccgcctgg
      721 aatccctctc cctccgggtc gccccgggag agttcttcat gatcgtgggt cccagcgggg
      781 caggaaagac cctgcttctc gaggccgtgg cggggcttcg gcccctttcc tccggcagga
      841 tactgattgc cgggaaagac gtcacggccg ttcctccgga gaagcgcggc gtgggcatcg
      901 tctaccagga ttacgccctc tttcctcatc ttaccgtgga ggagaatatc cggtggggcc
      961 tgaggttctc ccggaaggac ggcagcggga gcgtggacag gctcgtggac ctgctgcggc
     1021 ttcgccctct tctccgcagg tcgcccggga ccctttccgg aggagagcag cagcggacgg
     1081 ccctggcccg cgccctggcc gtgcagccat cggtgctcct cctggacgaa cctctttccg
     1141 ccctggaccc ggccttccgg gaagaattgc gggattacct cgcggaaatc aaccgggagg
     1201 ggatgaccct ggtgatggtg acccacgatt tcggggaggt cctctccctc ggcgaccggg
     1261 tcgctgttgt gtcggaagga agtctgcagc agaccgggaa cgtgcggacg gtgttcaggg
     1321 agccggccaa ccgtaccgtg gctgaattcg tcggcatgaa aaatatcttc cagtgcgaag
     1381 tgcggcacgg gaaagctttt cttggcaacg gggcggttct cgaggtggag gaatcatcac
     1441 ccgccggaac caggctcgcg ggcatacggc cggaggacgt ggccctcgtc agggatgttc
     1501 cggacggtac ggccaacgct tttcccggga ggatcgcctc ccttgttccc aggggaatgg
     1561 ctttcgaggt catcgtcgaa acggaggccg gaatccgcct ctcggcgagc gtcctgtcgt
     1621 ccgtcgtggc cgccggcggc gtccccgtgg ggggaaggtg cgtggccgca gcagccccgg
     1681 attcggttca tgttttttag gaaactcatc atatatgcgg aggtgttgtg tatgaaacgg
     1741 ttcaggtttt catcggttct ccttcttgct cttcttctga cgttttcacc cgggtgtttc
     1801 gcggcggaaa aggtgacggt gtaccacgcc ggcagtctcg cggcgcccat ggcgaagatc
     1861 gaggccctct acgaggccgc ccacccggat attgacatcc tgagggagtc gggcgggagc
     1921 gcggcccttg cccggaagat caccgacctc gggggcggat gcgacgtgtt cctgtcggcg
     1981 gactacatgg tcatcgagaa acttctccgt cccggggcgg cggactggaa cgctcttttc
     2041 gccagcaacg ccattgtgct catgtacgga ccgaagtcga aatacggcga cgagatcggt
     2101 ccggagaact ggaccggggt catgctgcgg ccggacgtgc ggtgggggca ctcggagccc
     2161 gacgccgatc cctgcggcta caggagcctc atggtgctcc agctcgcgga aaaattctat
     2221 ggaaagcagg gactctacga acaggccatg aagcaccagg ggcgggccgt ccgccagaag
     2281 gccatcgagc tcgtggccat ggttgagagc ggcaccatgg attacgcctt cgagtacaag
     2341 tccgtggcgg tgcagcacgg cctgaaatac cttgaactcc ccgtggagat caacctcatg
     2401 gaacccgccc tcgcggacgc ctactcggcc gcgtcggtgg aactggcggg aaaggagccg
     2461 ggcaagaaga tgacggtgaa gggagaaccc atcgtctacg gtctcaccat cccgaaagga
     2521 gcccccaacg caggcgcagc cctcgagctg gtgaaattca tccttgaccc ggagggcggc
     2581 cttgcggtct tcagggatat gggccag
//
LOCUS       DAOTHD010000229         2605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_46969_length_2605_cov_3.126275, whole genome shotgun sequence.
ACCESSION   DAOTHD010000229 DAOTHD010000000
VERSION     DAOTHD010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46969_length_2605_cov_3.126275"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="P5219_04925"
     CDS             complement(<1..304)
                     /locus_tag="P5219_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRX26130.1"
                     /translation="MRKILMMTAAVLILVFAGAAFADDVIRIGHTVSLTGGASMWGQS
                     EARALDMLVKKINDAGGVLGKKIEFVRYDNRNDGVEAVNVARRLVSESVDVTLPARP"
     gene            complement(429..1733)
                     /gene="lysA"
                     /locus_tag="P5219_04930"
     CDS             complement(429..1733)
                     /gene="lysA"
                     /locus_tag="P5219_04930"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRX26131.1"
                     /translation="MGQQNVNDMGHFLFGGWDVTELAERFGTPLYVLSEEGVRARCRA
                     VRESFMDRWEHSLAVYAGKAFLPLAMCRIIASEGLGLDVVSGGELHTAKSAGFPMERV
                     FFHGNSKTEEELRYAIDAGVGRIVVDGTSELDTLEETARKAGKRADILFRVTPGVDAH
                     THKYILTGHTGSKFGFPLLGNSLRTAVRRAMNSDCLSLRGFHFHIGSQIFENTSHVMA
                     VEIVVKTIAEFSRDLGFITMELNMGGGYGVEFDPEGKTPSIASFTDAMMETLASGCAA
                     EGIPLPRVIIEPGRWIVSEAGITLYTVQTVKTIDGMVTYAGVDGGMTDNPRPALYGAK
                     YWAVAAGKMDREPTETVTVAGKCCESGDVLIEGLKVPPLERGDLLAVLNTGAYTFSMA
                     SNYNRIPRPAALLVSPGRADVIAERQTYDDVLRWDRIPEHLR"
     gene            complement(2095..>2605)
                     /locus_tag="P5219_04935"
     CDS             complement(2095..>2605)
                     /locus_tag="P5219_04935"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRX26132.1"
                     /translation="RELLEKEAVTDALTGLYNRRGFLSFTEKALQLAARMGFRATFLF
                     ADLDGFKNVNDTLGHEEGDEVLRETAALLRDTFRASDITGRIGGDEFAVLMLHDGHDE
                     PGDPAGRLEEAVRRANGQRTREYRLSMSMGLSEWNPERPVSLGELMADADRRMYENKM
                     AGKGKKCGE"
BASE COUNT          457 a          880 c          718 g          550 t
ORIGIN      
        1 acgggcgcgc tggcagggtg acgtcgacgg attcggagac gagccggcgg gccacgttga
       61 cagcctcgac cccgtcattc cggttgtcgt agcggacgaa ctcgatcttc tttccgagga
      121 cgccgcccgc gtcgttgatc ttcttcacga gcatgtccag cgcccgggcc tcggactgcc
      181 cccacatgga agcgccgccg gtgagggaca cggtatggcc gatgcggatc acgtcatccg
      241 cgaaggcggc tccggcaaag acaaggatca aaactgccgc tgtcatcata aggatctttc
      301 tcatttctct tctcaacctc cctggtattt ggcgttcgtt cccaatccgc ttctccgccc
      361 cacaggggcg ggaatccttc cggcaccgtc gtttttccct ggccccacct ccggacatgg
      421 tgttccgttc accgaagatg ctccggaatc ctgtcccacc ggagcacatc gtcgtaggtc
      481 tgccgctctg cgatcacgtc ggcccttccc gggctcacca gcagggcggc aggccgcgga
      541 atcctgttgt aattgctcgc catggagaag gtgtaggccc cggtgttcag cacggcgagc
      601 aggtcgcccc gctccagggg aggaaccttc agcccctcga taagcacgtc cccggactcg
      661 cagcatttcc cggccaccgt caccgtctcc gttggttccc ggtccatctt gcccgccgcc
      721 acggcccagt atttcgcgcc gtacagggcg ggtctcgggt tgtccgtcat gcccccgtcc
      781 acaccggcgt aggtcaccat cccgtcaatg gtcttcaccg tctggacggt gtacagggta
      841 atccccgcct cggagacgat ccaccggccc ggctcgatga tcacccgggg aagggggatc
      901 ccctccgcgg cgcagcccga agcgagggtc tccatcatgg catcggtgaa ggaagctatg
      961 gaaggtgttt ttccttcggg gtcgaattcc acgccgtacc ctccgcccat gttgagttcc
     1021 atggtaatga agccgaggtc cctggagaat tcggcaatgg tctttaccac tatttccacg
     1081 gccatcacgt gggacgtgtt ctcgaaaatc tgggacccga tgtggaaatg gaagcccctg
     1141 agggaaaggc agtccgagtt catggccctc ctgactgccg tcctgaggga attcccgagg
     1201 aggggaaagc cgaacttgga gcccgtgtgg cccgtaagga tatatttatg ggtgtgggcg
     1261 tccacccccg gggtgacccg gaagaggatg tccgctctct tccccgcttt ccgggcagtc
     1321 tcctcaaggg tgtcgagctc ggaggtgccg tccacgacaa tccgccccac tcccgcatcg
     1381 atggcatagc ggagttcctc ttccgtcttg ctgttgccgt ggaaaaagac cctttccatg
     1441 gggaagcccg cggatttcgc cgtatggagt tcccctcccg ataccacgtc gagccccagc
     1501 ccttccgatg caatgatcct gcacatggcc aggggaagaa aggccttccc cgcgtacaca
     1561 gccagggaat gctcccaccg gtccatgaag gattccctca ccgccctgca ccgggcccga
     1621 accccctcct cggacaggac gtacagcggt gttccgaacc gctccgccag ctccgtcaca
     1681 tcccaacctc cgaagaggaa atgccccatg tcattcacgt tctgctgccc cactgcgcct
     1741 gcacctcttt ccgttcaaaa aacggggaat aaaaaaacga cccgccccca tgaagggacg
     1801 agtcgttgat ctcgcggtac caccctgctt gggcgcatgc tgcagcgccc cgctctcggc
     1861 cctgtgacgg gagcctcccg gaggcgtact tccggttcat tcgaaccgtt tcagccccgg
     1921 ctccgcagcc tttcgtccct ccgttcctgc ccgggaagcc tctcagcctg tgaacttccc
     1981 tctctggcgg cagatccatt acttcgggca ttctgcatca tcgcctgtgc tttttttgtt
     2041 tcggacatta tatccagtgg ggggatgaac gtcaacacaa aaagaggaaa ttcatcattc
     2101 tccgcatttt tttcccttcc cggccatctt gttctcgtac atcctccggt cagcatcggc
     2161 cataagctct cccagggaaa cgggacgctc ggggttccat tcgctcaggc ccatgctcat
     2221 ggacagccgg tactccctgg tcctttgccc gttggcccgt cggacggctt cttcgagtct
     2281 ccccgccggg tcgcccggct cgtcatgccc atcgtgaagc atgagtacag caaactcatc
     2341 ccccccgatc cttcccgtga tatccgatgc ccggaacgta tcccgaagaa gagcggccgt
     2401 ttcccggagg acttcgtccc cctcctcgtg accgagggtg tcgttcacgt tcttgaagcc
     2461 gtccagatcg gcgaaaagaa aagtcgccct gaagcccatc ctggcggcga gctgcagcgc
     2521 cttttcggtg aaggagagaa atcctcttct gttgtagagc cccgtcagtg cgtcggtgac
     2581 cgcttccttt tcgaggagtt cccgg
//
LOCUS       DAOTHD010000230         2602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_47047_length_2602_cov_2.601492, whole genome shotgun sequence.
ACCESSION   DAOTHD010000230 DAOTHD010000000
VERSION     DAOTHD010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47047_length_2602_cov_2.601492"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..746
                     /locus_tag="P5219_04940"
     CDS             159..746
                     /locus_tag="P5219_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HRX26133.1"
                     /translation="MEQRLLLHACCAPDATVPLADLSAEGYAVTLYWYGANIHPAGEE
                     EKRREALASLSGGNVPVIVEDAFTPPWMETVSPLAGEPEGGRRCALCFRLQLEGAARA
                     AVREGISLLCTTLTISPHKDAGLINAIGEETARTFGLRWLFRIFRKQNGFVRSVAMSK
                     ELGLYRQSYCGCIFSLRRGEAHESVSGGETSPGSA"
     gene            703..1680
                     /locus_tag="P5219_04945"
     CDS             703..1680
                     /locus_tag="P5219_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRX26134.1"
                     /translation="MNQSPVGKLPPGQLEGSIFRFCGASRPEVLIGPGIGEDAALIRW
                     PEGKYVAVASDPIVGASAGAGKFLVHINANDIACKGGDPSFFVVTLLVPSRQGIPMVE
                     ALMREIHEACLSIGAAVVGGHTELTDRYDQPVLVGTMMGPTEYVHRATDIRPGDRLIL
                     TKHAGLEGMAILASDRPDLLEPVIGREGVREAAGWLDGISVLEEAKCLRDIARFLHDP
                     TEGGIGGGIAEIMRLSSLGAELDYGKIPLSPLTERARQGLGFDPFHLISSGVLLAVVA
                     PEHEEEALARLAKKGIPAASAGRFVEGRGNCPDSVAEELWGLLGRPKEE"
     gene            1685..>2602
                     /locus_tag="P5219_04950"
     CDS             1685..>2602
                     /locus_tag="P5219_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HRX26135.1"
                     /translation="MSSGTIMGRIARLRELLSRERADVFLLFVAERYNSESMTYLSGF
                     RGSSGALVISQDRQYLVTDGRYALQARAESPLDVLILEGVSLQGKAAEILRQGGWKTA
                     GFEAERVPVSFFRGLESSVSSWKDCSGLLPALRRRKDETEAALIRTAGDLAYDSYLEV
                     LGAVREGMTEKEFNALLEYTLRKNGAEGGWTNNGFIVASGERSALPHGAPTGRGFRKG
                     DMVTVDFGATVEGYMSDLTRNFSLGPLSPQGRDIQDTLLEAHLRAAEALRPGVEGKAV
                     DAVARDVVTRRGWGKHFPHGLGHGLGLEVH"
BASE COUNT          464 a          813 c          868 g          457 t
ORIGIN      
        1 ccggatgact gaactggggc tcagcgcatc cttcagggcc gtgggtgtgc acacctacgg
       61 ggaatccggc agctccaccg acgtcatcgc cgccatgggc ctcgacgaga tctccctggc
      121 gaaagccttc gagtccctgc cgggaaaata ggcggtccgt ggaacagcgg cttcttctcc
      181 acgcgtgctg tgctccggac gccacggtgc ctctcgccga tctttcggcg gaagggtatg
      241 ccgtgacgct gtactggtac ggtgccaaca tccaccccgc gggagaggaa gaaaaacgga
      301 gggaggccct ggcctccctc tcggggggaa acgtgccggt catcgtggag gatgctttca
      361 cccctccgtg gatggaaacg gtctcgcccc ttgcggggga gccagagggg ggcaggcggt
      421 gcgctctctg cttcaggctc cagctcgagg gagcggcgcg ggcggctgtc cgggaaggga
      481 tttctttgct ctgcaccacc ctgaccatca gccctcacaa ggatgcgggc ctcatcaacg
      541 ccattgggga ggaaacggcc cggaccttcg gcctccggtg gctcttcagg attttccgga
      601 agcaaaacgg ctttgtccgc tccgtcgcca tgtcgaagga gcttggtctt tacaggcagt
      661 cctactgcgg ctgcattttc agcctgcgaa gaggtgaggc acatgaatca gtctccggtg
      721 gggaaacttc ccccgggtca gcttgaagga agcatcttcc ggttctgcgg ggcatcccgg
      781 cccgaggtgc ttatcggtcc cggcataggg gaggatgccg ccctcatccg gtggccggaa
      841 ggaaagtatg tggccgtggc ctccgacccc atcgtcggcg cgtccgccgg ggcaggcaag
      901 tttctcgtcc acatcaacgc caacgacatc gcctgcaagg gaggagaccc ctcatttttc
      961 gtggtcaccc tcctcgttcc ctccaggcag ggcattccca tggtggaggc cctcatgagg
     1021 gagatccacg aggcctgcct ctccataggg gcggcagtgg tgggaggcca cacggaactt
     1081 accgaccgct acgaccagcc cgtcctcgtg gggaccatga tgggccccac ggaatatgtc
     1141 catcgggcca cggacatccg cccgggggac aggctgattc ttacgaaaca cgccggtctc
     1201 gagggcatgg ccatcctcgc ctccgaccgt cctgacctcc tggaacccgt catcggacgg
     1261 gagggtgtcc gggaagccgc cgggtggctg gacggcatct cggtcctcga ggaagcgaag
     1321 tgcctccggg acatcgcccg gttcctccac gaccccaccg aggggggcat cgggggcggc
     1381 atagcggaga taatgaggct cagcagcctc ggcgcggagc tggattacgg aaaaatcccc
     1441 ctgtcacccc tgacggagcg ggcgcggcag ggcctggggt ttgacccctt ccatctcatc
     1501 tcctccgggg tcctcttggc ggtagttgct cccgaacacg aggaggaagc ccttgcgagg
     1561 ctggcaaaga aaggcattcc cgccgcttcg gcaggccggt tcgtcgaggg gcggggaaac
     1621 tgtcccgaca gcgtggccga agaattgtgg ggtttgctcg gccggccgaa ggaggaatga
     1681 ctccatgagt tccgggacca tcatggggag gatcgcccga ctgagggaac ttctttcacg
     1741 ggagagggcg gacgtgtttc tcctcttcgt ggcggagcgg tacaacagcg aatccatgac
     1801 gtacctgagc ggtttccggg gaagcagcgg cgccctcgtt atctcccagg acaggcagta
     1861 cctcgtcacg gacgggcggt acgccctcca ggcccgggca gaatctcccc ttgacgttct
     1921 catcctcgag ggggtatccc tccagggaaa agcggcggag atcctgcggc agggcgggtg
     1981 gaaaaccgcg ggtttcgagg ccgaacgggt cccggtctcc tttttccggg gactcgaatc
     2041 ttcggtgtcg tcgtggaaag actgctcagg ccttcttccg gccctccgcc ggagaaagga
     2101 cgaaaccgaa gccgccctca tcaggaccgc gggggacctt gcctatgatt cctaccttga
     2161 agtcctgggg gccgtccggg aaggcatgac ggagaaggag ttcaacgccc tgctggaata
     2221 taccctgagg aaaaacggcg ccgaaggagg atggacgaac aacggcttca tcgtcgcctc
     2281 cggtgaacgg agcgccctgc cccacggagc gcccaccgga cggggctttc ggaaaggaga
     2341 catggtcacg gtggacttcg gtgccaccgt ggaagggtac atgtccgacc tgacgaggaa
     2401 cttttctctc ggtcccctct ctccccaggg cagggacatc caggacaccc tcctggaagc
     2461 ccacctgcgg gccgcggaag ccctccgtcc cggtgtggaa ggcaaggccg tggacgccgt
     2521 cgccagggac gtggtcaccc ggcggggatg ggggaagcat ttcccccacg gcctcgggca
     2581 tggtctcggg ttggaggttc ac
//
LOCUS       DAOTHD010000231         2600 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_47087_length_2600_cov_3.621611, whole genome shotgun sequence.
ACCESSION   DAOTHD010000231 DAOTHD010000000
VERSION     DAOTHD010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47087_length_2600_cov_3.621611"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            422..877
                     /locus_tag="P5219_04955"
     CDS             422..877
                     /locus_tag="P5219_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26136.1"
                     /translation="METSIVAVCRCPEDGSYSTAYESDIVRNKQYCLGDIQSVLENKA
                     DYCLASPRTRSYWRSWLRTMWASVVEKIHRYIGRSLSECDISCSLAAFLKGCGDQWLR
                     YVLDLFYTQSNNLCILVELVGPTIGPRGEQLQTALAEEAAEQQKPPRGG"
     gene            883..2322
                     /locus_tag="P5219_04960"
     CDS             883..2322
                     /locus_tag="P5219_04960"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HRX26137.1"
                     /translation="MELKDLRPNEQEKYNAAREVIDLGCTDTAIRRGRAKLGCTRKTL
                     LKYVQWCKSEDLSRFSHRNKGRKPATTIPQDTRELVERLYKDTYCTASFTHFLEILKE
                     DHGISMSDGTVHSILKQAMLVSPCSKKATKRLMEKKLRMLARKEHLSKAESEQINAAL
                     AILDDADAHPRRPRSKYFGEMIQMDASELVWAKGAGKWHLHVAVDDATSEVVGAWFDT
                     QETLNGYYRVLYMILKLYGIPSRFRTDRRTVFEYNSLSRPDEEKDTFTQFAAACKTLG
                     IELEATSVPQRKGRVEKMNKTLQGRIPVELLRHGITTMEGANAFLWDYLPRFNAQFSL
                     KDEKDLASSTFLQAPGEADINAILAVVSQRVVDAGSSIKYHNAYYQPYAQLSGGLRPK
                     LFAKGTKALVVKAFDWTLLASIKDATYLLREVEKRSSHSKEFDPERPPAPRQRKSSTP
                     APDHPWRTRFLAPNVLECHIAKPREDYES"
     gene            2319..2504
                     /locus_tag="P5219_04965"
     CDS             2319..2504
                     /locus_tag="P5219_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26138.1"
                     /translation="MRWREKQETLQSLREEHSFSSDEYETLVQNINEELEQASSDILR
                     AWEKEARKARNTGCYGF"
BASE COUNT          658 a          725 c          744 g          473 t
ORIGIN      
        1 gaattcttct aaactgttgt tgcagtttat tataacttcg ctttccgggt attacaataa
       61 aaactcgttc ttttaacact ggtcactgtc tcggatatga attcttccaa tcattcctta
      121 aatgatttat tcctgcctat gagaaagatg tggtttaatg gttggtaaga actgggagag
      181 catccccgaa ggagaaaaaa gaagaggaag tcccttgtct tgttggcgct tgataagact
      241 tcctctatct caaaatacgt atggaaacaa taccatcgaa gcggcttagg ttcaagagca
      301 acaccatcca tccttgagga agggcaacgg catgatgctg tgccgcacat gcctcggggc
      361 gctggagtat gccttctcgc gcaagagggg caggcggtac acggctgagg aaggtccggc
      421 ggtggaaacg agtatcgttg ccgtctgccg ttgcccggag gatggaagct actcgacagc
      481 ctatgaatcc gacatcgtca ggaacaagca gtactgcctg ggcgacatac aatccgtgct
      541 ggagaacaag gcggactact gcctggcaag cccgcgcacg agatcctact ggcggtcgtg
      601 gctgcgcaca atgtgggcgt cggtggtcga gaaaattcat cgttacatcg gccgcagcct
      661 ttctgaatgc gacatttcct gctcccttgc ggccttcctc aaggggtgcg gggatcagtg
      721 gctgcggtac gtgctggacc tcttttacac ccaatccaac aacctttgca tcctcgtcga
      781 gctcgtcggc cctacgatcg gccccagggg cgaacagctg cagacagctc tcgctgaaga
      841 ggctgcagaa cagcaaaaac cgccccgggg aggatgaaag agatggaatt gaaggatttg
      901 aggccgaacg agcaggaaaa gtacaacgcc gccagggaag tcatcgacct cggctgcacc
      961 gacacggcaa tccgaagggg ccgggcgaag ctgggatgca ccagaaagac gcttctgaag
     1021 tacgtgcagt ggtgcaaaag cgaagacctg tcgcggttca gccaccgcaa caagggcaga
     1081 aagcccgcca ccaccatacc gcaggatacg agggaactgg tcgagcggct gtacaaggac
     1141 acctactgca ccgccagctt cacccatttt ttggagatac tcaaagagga ccacggcatc
     1201 agcatgtcgg acgggacggt gcactcgatc ctcaagcagg ccatgctcgt ctcgccctgc
     1261 tcgaagaagg cgaccaagag gctgatggag aagaaattga ggatgcttgc acggaaggag
     1321 cacctgagca aggccgaaag cgagcagatc aatgccgccc tggctatcct ggacgacgcc
     1381 gatgcgcatc cgcgcaggcc gcgaagcaag tatttcgggg agatgatcca gatggacgcc
     1441 agcgagctgg tctgggccaa gggggccggg aaatggcacc tgcatgtggc cgtcgacgat
     1501 gcaaccagcg aggtggtggg ggcgtggttc gacacccagg agacgctcaa cggctactac
     1561 cgcgtgctgt acatgatcct caagctctac ggcatcccct ctcggttcag gaccgaccgc
     1621 aggaccgtct tcgagtacaa ctccctctcc aggcccgacg aggagaagga caccttcacc
     1681 cagttcgcag cggcctgcaa aaccctgggc atcgagctcg aggccacctc cgtcccccag
     1741 cggaagggcc gtgtcgagaa aatgaacaaa acgctccaag gccgtatccc ggtcgagctt
     1801 ctgcgccacg ggatcaccac catggagggg gcgaacgcct tcctgtggga ctatcttccc
     1861 cgcttcaatg cacaattctc gctcaaggac gagaaggatc ttgcaagcag caccttcctc
     1921 caagcccccg gcgaggccga catcaatgcc atcctggcgg tggtctccca acgggtggtc
     1981 gatgcgggca gcagcatcaa gtaccacaac gcctactacc agccctacgc ccagctttcc
     2041 ggcggactac gcccgaaact cttcgccaag ggtacgaaag cccttgtcgt caaggccttc
     2101 gactggacgc tgctggccag catcaaagat gcgacatacc tgctcaggga agtggagaag
     2161 agaagcagcc acagcaagga attcgacccg gaaagacccc ctgcacccag gcaaaggaag
     2221 tcttccacgc ctgcaccaga ccatccctgg agaacaagat ttctcgctcc gaatgtcctg
     2281 gagtgccaca tcgccaagcc gagggaggac tatgagtcat gagatggcgg gagaaacagg
     2341 aaaccctgca gagtctcagg gaggagcatt ccttctccag cgacgagtac gaaacgctgg
     2401 tgcagaacat caacgaggag cttgaacagg cttcctcaga cattctccgg gcctgggaaa
     2461 aggaggcccg gaaggcccgg aataccggct gttatggatt ctagaaggag gaaaaaatca
     2521 tttacgggct acacaaaata gtgtgaaaga atcgttaatt tgtgtaaaag gcaattgtga
     2581 agccgatgta gaccaaatgg
//
LOCUS       DAOTHD010000232         2599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_47121_length_2599_cov_2.777909, whole genome shotgun sequence.
ACCESSION   DAOTHD010000232 DAOTHD010000000
VERSION     DAOTHD010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47121_length_2599_cov_2.777909"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="P5219_04970"
     CDS             <1..1051
                     /locus_tag="P5219_04970"
                     /inference="COORDINATES: protein motif:HMM:NF022727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3084 domain-containing protein"
                     /protein_id="HRX26139.1"
                     /translation="SLFGLSPRDTSRLITAITGMFISIAILITMTVISENVRTALFSM
                     KFIQGQLQSLTRDLQESRSESQLMALNLLDSEKRLKEQEEKLLSVQKELATAQPLLEE
                     IRQSLDTTQKERDQLEEEKKALAASVEELKTEAEELRKGLIQIRSGRIAVFAKEILGQ
                     MAVEPMSGRSEAEQIFQDLRRQAEYVIAARTGLSPSEIVLAADIEKEMESIAACTEQQ
                     GRKFVRTVAVANAVFGEDIRITYEVSDSVLVYRKGEVLYVQAVDPGTVGENAEAELHL
                     ILRMVNRKAVQDSIKPDPATGKVGALDATEFFDAVDTIRKAEGKLTVEISAAEDIYTE
                     GPVRVAISIRPAEEQ"
     gene            1069..1287
                     /locus_tag="P5219_04975"
     CDS             1069..1287
                     /locus_tag="P5219_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HRX26140.1"
                     /translation="MLIVACQVCGESKVIAGTPDADGMARATWICPCCGTGQVVQLPV
                     SSDARKTDLRKIVCGMALAGRPADEDRS"
     gene            1284..1703
                     /locus_tag="P5219_04980"
     CDS             1284..1703
                     /locus_tag="P5219_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRX26141.1"
                     /translation="MNGGYFFYDSKHAVSPEELANLYRFTRWGKSRSQDDIARMLEGT
                     SMCFSVRHDDHLVAFCRILTDFVYRASLWDIMVHPDHQGKKVGTSLLDYALGHPAVKH
                     IPLIITYTSELSSFLTPHGFAPKEGALMLLRRPIEYS"
     gene            1690..2316
                     /locus_tag="P5219_04985"
     CDS             1690..2316
                     /locus_tag="P5219_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRX26142.1"
                     /translation="MSIPEAVLRFGTHLLWPTACPFCGALGVPACVTCLLPLLAPPLP
                     ADLGSLTLEAGGIHEGILRDLVLELKYGRNRPLGVVMGRALGRIFPVPPCDILVPVPL
                     HADSGRGYNQSRALAEGISREWGVPVADGLQWNGLRTAQTSLEEGERRRMPAGAILPR
                     GKELAGKRAVLVDDVSTTGTTLLRAADAAARGGGRAVLALAWTIAPKA"
     gene            2377..>2599
                     /locus_tag="P5219_04990"
     CDS             2377..>2599
                     /locus_tag="P5219_04990"
                     /inference="COORDINATES: protein motif:HMM:NF016223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HRX26143.1"
                     /translation="MIEKIPGVYGPKAVNKINVKKSYQSASGEGGRDGVEFSSFAVEL
                     SKVSAELKKIPDVREDLVEGFRKQIQEGTY"
BASE COUNT          550 a          831 c          783 g          435 t
ORIGIN      
        1 ctccctcttc ggccttagcc cccgggacac cagccgtctc atcaccgcca tcaccggaat
       61 gttcatctcc atcgccatcc tgatcaccat gaccgtcatc tcggaaaacg tccgcacagc
      121 cctcttcagc atgaaattta tccaggggca gctccagtcc ctcaccaggg acctccagga
      181 aagccggagc gaatcccagc tcatggccct caaccttctg gacagcgaaa agcgcctcaa
      241 ggagcaggag gagaagctcc tctccgtcca gaaggaactc gccaccgccc agccccttct
      301 cgaggaaatc cggcagagcc ttgataccac ccagaaagag cgggatcagc tcgaagaaga
      361 aaagaaagcc ctcgccgcct ccgtggaaga gctcaagacc gaggccgagg agctcaggaa
      421 gggccttatc cagatccgga gcggccgcat cgccgtcttc gccaaagaga tcctcggcca
      481 gatggccgtg gaacccatga gcggccggtc ggaggcggag cagatcttcc aggatctgcg
      541 gcgccaggcg gaatacgtca tcgccgccag aacaggtctc agcccctcgg agatcgtcct
      601 cgccgccgac atcgaaaaag aaatggagag catcgccgcc tgtacggaac agcagggccg
      661 gaaattcgtc cgcaccgtcg ccgttgcgaa cgccgtcttc ggggaggaca tcaggataac
      721 ctacgaggtc agcgacagcg tgctcgtcta ccggaagggt gaagtcctct atgtccaggc
      781 cgtggatccc gggacggtgg gcgagaacgc cgaggccgag ctccatctca tactgcggat
      841 ggtgaacaga aaggcagtcc aggattcaat caaaccggac cccgccaccg ggaaagtggg
      901 cgccctggat gccacggaat ttttcgatgc ggtggacacc atacgaaaag cggaagggaa
      961 actcacagtt gagatttccg cggcggaaga catctatacg gaaggtcccg tgcgggtggc
     1021 aatctccata cgcccggcgg aggaacaata aaggctggag gctgaaaaat gctgatcgtg
     1081 gcgtgccagg tttgcggaga atcgaaggtc atcgcgggaa ccccggacgc ggacggcatg
     1141 gcgagggcca cctggatctg cccgtgctgc ggtaccgggc aggtggtgca gcttcccgtg
     1201 tcgtcggacg cccggaaaac cgacctgcgg aaaatcgtgt gcggcatggc ccttgccggc
     1261 cggccggctg acgaggacag gtcatgaacg gcgggtactt tttctacgac agcaagcacg
     1321 ccgtctcccc ggaggagctg gccaacctct accggttcac ccggtggggc aagagccgtt
     1381 cccaggacga catcgccagg atgctggaag gcacgtccat gtgcttcagc gtacggcatg
     1441 acgatcacct tgttgccttc tgccgcatac tcaccgactt tgtctacagg gcttccctgt
     1501 gggacatcat ggtccacccg gaccaccagg ggaagaaggt gggcacctcg ctgctggatt
     1561 acgccctcgg ccacccggcg gtcaagcaca ttcccctcat catcacctac accagcgaac
     1621 tctcttcctt cctcaccccc cacgggttcg ccccgaagga aggggccctc atgcttctcc
     1681 ggaggcccat tgagtattcc tgaggcggtc ctccgcttcg gaacccacct cctttggccg
     1741 accgcctgcc ctttctgcgg ggccctgggt gtgccggcct gcgttacctg tcttctgccc
     1801 cttctggcgc cgccccttcc ggcggacctc ggctccctga ccctcgaggc gggaggaatc
     1861 cacgaaggaa tactccggga cctcgtcctc gaactgaaat acggccggaa ccgtcccctc
     1921 ggcgttgtca tgggccgggc cctcggcagg atatttccgg ttcctccctg cgatattctc
     1981 gtacccgtac cactccacgc cgacagcgga aggggataca accagtcccg ggccctcgcc
     2041 gagggaattt cccgggaatg gggcgttccc gtggcggacg gcctgcagtg gaacggcctc
     2101 cggacagccc aaacctccct ggaggaaggg gagcgaaggc gcatgcccgc cggggcgatc
     2161 cttccccggg gaaaagaact tgcgggaaag agagccgtgc tcgtggacga tgtctccacc
     2221 acgggcacca cactgctccg ggccgccgac gccgcggccc gcggaggagg aagggcggtt
     2281 ctcgcccttg cctggaccat tgcccctaaa gcctgaaagg ttgtcgtccg ataaccattg
     2341 tgaagagaaa agtttctcct gtacggggtg atcgacatga ttgagaagat tcctggagta
     2401 tacggaccga aagcggtaaa caagatcaac gtgaagaaaa gctaccagtc ggcctccgga
     2461 gaggggggca gggacggcgt ggagttcagc tccttcgccg tggagctctc caaggtctcg
     2521 gcggagctga agaagattcc cgacgtccgc gaagatcttg tcgaaggctt cagaaaacag
     2581 atccaggaag ggacctacg
//
LOCUS       DAOTHD010000233         2583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_47472_length_2583_cov_5.225870, whole genome shotgun sequence.
ACCESSION   DAOTHD010000233 DAOTHD010000000
VERSION     DAOTHD010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47472_length_2583_cov_5.225870"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2086
                     /locus_tag="P5219_04995"
     CDS             <1..2086
                     /locus_tag="P5219_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26144.1"
                     /translation="GESMHMLADMTQPAHVRNDSHPADEPIEAGTFSDHVRRAAGAPF
                     VDSRIRPFLASAGGRLQEPAELFHRVASFVNGTFYSLDTIYDKESKVLPNNRQKGYPS
                     PQLRDLAVEKKKIPGFVGEREVRRFYGVFDSERAPMIQDRLSFHWFDPEQSFVSDFEG
                     AKEKGGRAVAAGLGRLGPFQVPHAFAAGQGRILLPVAIHACADLMNLFYPTLELRAEF
                     EEMESEMEGVDPRATPVRIIEVKASMKHHAEKDPAWKANGLTILYSGPALLVVREKDK
                     TVYERKILFVKGEPKQIETADGTMEEAPVRLFAAGTAGPLSEEELFYEIQPGQEAFLR
                     IEAGSRVFESVPWKEEDKTEVTVLPPRVLVLELIDDASEAEHEFEAAAVPKKPHLFEW
                     DFGDGSPVVEDRKNPGENSVLVHRFTGLKPGDSFTPAVRLLDGKGRVLAEDSVSVTVL
                     KGEKRHFFENRTFWMTGSPLGNDPTADLPTVPLVLSPSISGIDGEIPPDSPEGKRLAQ
                     LTEGVAKAGDSMKEQMAKEMAKMGISLSDAQMAEALKQALSMAGLGGQDGPVQRRPVL
                     GSGWQHEGRPVTLSLAVSIGAIPPLRLYMRGEKEQPEPVEARMAVRNWFFTTGTQSSS
                     SVQGSGGSASVSWTPEKGKTPESFALDLVVFYSLEFFKRTGEPWGNDGAPLRYENMSV
                     SFPVGVYFCPVK"
     gene            2135..>2583
                     /locus_tag="P5219_05000"
     CDS             2135..>2583
                     /locus_tag="P5219_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26145.1"
                     /translation="MNCFLSPAFHRLFRREREPAAFLRRILPASIVLVLLLARAFPAP
                     AAEVPSPEDVPPGMTLRWVPPFYITVPSDWKPMLDRDGLGFYTGAHPEAMDDPSLADL
                     PVVALGVTRQKAPKGGGYRAFFEEMERSAAKDKAKNFTSKEEEITIGS"
BASE COUNT          545 a          765 c          805 g          468 t
ORIGIN      
        1 cggggaatcc atgcacatgc ttgccgacat gacccagccg gcccacgtgc ggaacgactc
       61 ccacccggcg gacgagccca tcgaggcggg gaccttcagc gaccatgtcc ggcgggcggc
      121 gggggccccc ttcgtcgact ccagaatccg tcccttcctc gccagtgccg gaggcaggct
      181 ccaggaaccg gcagaacttt tccaccgtgt tgcctccttc gtcaacggaa ccttctattc
      241 cctggacact atttacgaca aagaatcgaa ggttctgccg aacaaccggc agaagggata
      301 cccttccccc cagctgagag acctggcggt ggaaaaaaag aagattcccg gtttcgtggg
      361 tgaaagggaa gtccggagat tctacggtgt cttcgacagt gaacgggcac ccatgatcca
      421 ggaccgcctc agtttccact ggttcgatcc ggagcagtcc ttcgtgtcgg attttgaggg
      481 ggccaaggaa aaggggggga gggcggttgc cgcgggcctg ggaaggcttg gtcccttcca
      541 ggttccccac gccttcgccg ccgggcaggg cagaatcctc ctgcccgtag ccatccatgc
      601 ctgcgcagac ctcatgaacc ttttttatcc caccctcgag ctccgggcgg agttcgagga
      661 aatggagtcg gagatggaag gggtcgatcc ccgggccacc cctgtacgga tcatagaagt
      721 gaaggcttca atgaagcacc atgcggaaaa ggatcccgca tggaaagcca acgggctgac
      781 aatcctgtac tcaggaccgg ccctgctcgt ggtgcgggag aaggacaaaa cggtttacga
      841 gagaaaaatt ctgttcgtga agggcgagcc gaagcagatc gagacagcgg acggaacaat
      901 ggaggaagct cccgtcaggc ttttcgctgc cggaaccgca ggcccgctga gtgaggagga
      961 actcttctac gagatacagc ccggccagga ggccttcctg agaatcgagg cgggaagccg
     1021 ggtctttgaa agcgtaccat ggaaggaaga ggacaagacc gaagtcaccg tccttcctcc
     1081 ccgggtcctg gtgctggagc tgatcgacga cgcctcggag gcggagcacg aattcgaagc
     1141 tgcggcagtg ccgaagaagc ctcacctgtt cgagtgggat ttcggcgacg gaagccccgt
     1201 ggtggaggac cggaaaaatc ccggagaaaa ctctgtgctg gtccaccgct tcaccggcct
     1261 gaagcccggg gattccttta ctcccgccgt ccggctgctt gacggaaaag gcagggtcct
     1321 cgcggaggat tccgtctccg tcaccgtgct gaaaggagaa aaacggcact tctttgaaaa
     1381 ccggaccttc tggatgaccg gttctcccct ggggaatgac ccgacggcgg accttcccac
     1441 cgtgcctctc gtcctgtccc cgtcgatctc gggaatagac ggcgaaatcc ccccggacag
     1501 tccggaagga aaacggctgg cccagctcac cgaaggcgtg gcaaaggccg gggactccat
     1561 gaaggaacag atggcgaagg aaatggcgaa aatgggaatc agcctgagcg atgcccagat
     1621 ggcggaggcg ctgaaacagg ccctttccat ggccggtctc ggaggacagg acggcccggt
     1681 ccagaggcgt cccgtccttg gaagcgggtg gcagcatgaa ggccgcccgg tgacactgtc
     1741 cctcgccgtt tccatcggag ccatacctcc gctccggctg tacatgcggg gcgaaaagga
     1801 gcagcccgag cccgtggagg cccggatggc cgtacgaaac tggttcttca ccacgggaac
     1861 ccagtcaagc tcctcagtcc aggggagcgg cggatccgct tccgtttcat ggacgccgga
     1921 aaagggaaag actcccgagt cctttgccct ggacctggtg gtgttctatt ccctggagtt
     1981 tttcaaaaga accggtgaac cctggggcaa tgacggtgct cccctcaggt acgagaatat
     2041 gtccgtctcg ttccccgtgg gggtgtactt ctgcccggtg aagtgacggg gcacacgggg
     2101 aatgtgtatc atgcagagaa ggaggaagtt ttcgatgaat tgttttcttt cacccgcttt
     2161 tcaccgtctc ttcaggagag agagagagcc ggcggctttc ctccgccgga ttctcccggc
     2221 ctccattgtc cttgtgctgc tccttgccag ggcctttccc gccccggcag cggaggtccc
     2281 ttccccggag gatgttcccc ctggaatgac cctccggtgg gtccctccct tttacataac
     2341 cgttccctcg gactggaagc ccatgctgga ccgtgatgga ttggggttct acaccggggc
     2401 acatcccgag gcaatggacg acccgtccct ggcggacctt ccagtggtag ccctgggcgt
     2461 gacccggcag aaggccccga agggcggcgg ttacagggcc tttttcgagg aaatggaacg
     2521 aagcgcggcg aaggacaagg caaaaaattt cacgtcaaag gaagaagaaa tcaccatcgg
     2581 atc
//
LOCUS       DAOTHD010000234         2583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_47474_length_2583_cov_3.866693, whole genome shotgun sequence.
ACCESSION   DAOTHD010000234 DAOTHD010000000
VERSION     DAOTHD010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47474_length_2583_cov_3.866693"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1613)
                     /gene="ftsH"
                     /locus_tag="P5219_05005"
     CDS             complement(<1..1613)
                     /gene="ftsH"
                     /locus_tag="P5219_05005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRX26146.1"
                     /translation="MEKKTQHTGRSRESGRFQPFLRKNSPSGGGQPGKNLPPSRIWLW
                     FLAVLAVNYLVASLLTPAPKEPVNIPYTLFREETAKGNVHAIYNRGEIVWGDFRNPVT
                     YPGAGTETGPSGSGAKAVTLFTTTLPAFVDSGLEAFLLSHGVEISAKPMHEERSAWET
                     LLYGFGPGLLFILFYFWLFRRSASQGGMGGLMGLGKSKARRYDQEGEEKVTFNDVAGI
                     DEAENELVEIVDFLKDPEKYTRLGGTAPKGILLVGAPGTGKTLLAKAVAGEAGVPFYS
                     MSAAEFVEMIVGVGAARVRDLFSQARKNAPAIIFIDELDAIGRARGQVSIGGSSEQEQ
                     TLNQILTEMDGFSSRTGVIVLAATNRPDVLDKALLRPGRFDRRVVVNLPDRPGREAIL
                     KVHTRNVPLSGETSLDEIAATTPGFSGADLKNLVNEAALLAARRGQDSVTAKDFLDAL
                     EKIVLGPERPLLLSTEDRERVAYHEGGHAILGLTVPGADPVHRVTIMPRGQALGVTYQ
                     RPVSDRYSYPEAYLRARIIGILGGRAAEEV"
     gene            1874..>2583
                     /locus_tag="P5219_05010"
     CDS             1874..>2583
                     /locus_tag="P5219_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRX26147.1"
                     /translation="MNSDFFNLYSHDFIRVAACVPAVRTADPSHNAERTVELLREASD
                     RRAVLAVFPELGISAYSCGDLFFQDALLQSALKGLETILGASASLDMVIAAGVPLKIG
                     DGLFNCAAVLFRGRVLGVAVKSFLPNYREFYELRHFRPASALPSDTLDLLGQKDIPVG
                     ADLIFEAEGIPGFRLFCEICEDLWTPVPPSCYAALSGATVAVNLSASNVTTGKADYRR
                     ALVANQSARCIAAYVYAGT"
BASE COUNT          420 a          841 c          788 g          534 t
ORIGIN      
        1 acctcttccg ccgccctgcc gccgaggatg cctatgatcc gtgcccggag ataggcttcc
       61 gggtagctgt accggtcgga gacagggcgc tggtatgtga cccccagggc ctggccccgg
      121 ggcatgatcg tcaccctgtg aacggggtcg gcgccgggga ccgtcaggcc gaggatggcg
      181 tgtccccctt catggtacgc cacccgctcc ctgtcctcgg tactcaggag aaggggccgt
      241 tccggtccga gcacgatttt ttcgagggca tccaggaaat ccttcgcggt cacgctgtcc
      301 tgtccccgcc tggcggcgag aagggcggcc tcgttgacga ggttcttcag gtcggccccg
      361 gaaaagcccg gagtggtggc ggcaatctcg tcgagactgg tttccccgga caggggaacg
      421 ttccttgtgt gtaccttcag gatggcctcc cgccccggcc tgtcgggaag attgaccacg
      481 acccgccggt cgaaccgccc cggcctcagc agggccttgt cgagcacatc gggccggttg
      541 gtggccgcga ggacgataac cccggtcctg cttgagaaac cgtccatctc ggtcaggatc
      601 tggttcagtg tctgctcctg ctcgctcgag cccccgatgc tcacctggcc cctcgcccta
      661 ccgatggcgt ccagttcatc gatgaagatg atggcgggcg cgtttttccg tgcctggctg
      721 aaaagatccc gcacccttgc tgctcccacg ccgacgatca tctctacgaa ttccgcggcg
      781 ctcatcgaat aaaagggcac tccggcctcg ccggccaccg ctttcgccag cagggttttt
      841 cccgttcccg gcgctcccac gagaagaatg ccctttgggg cggttccacc cagcctggtg
      901 tatttttcgg gatccttcag gaaatcgacg atctccacaa gctcgttctc tgcctcgtcg
      961 atgcccgcca cgtcgttgaa ggtgaccttt tcttcaccct cctggtcgta ccgccgggcc
     1021 ttgcttttcc cgagccccat gaggccgccc atgccgccct gggaggcgga gcggcggaac
     1081 agccagaagt agaagaggat gaacagcagt cccgggccga aaccgtagag cagggtctcc
     1141 caggcgctcc gctcctcgtg cattggtttc gcgctgattt ccacgccatg ggagagcaga
     1201 aacgcctcca ggccgctgtc cacgaatgcc ggaagggtgg tggtgaagag tgtgactgct
     1261 ttcgccccgc tcccggaagg acctgtttcc gttccggccc cggggtacgt cacggggttc
     1321 cggaaatccc cccagacgat ctctcccctg ttgtagatgg cgtggacgtt ccctttcgcc
     1381 gtttcctccc tgaagagggt atagggaatg ttcaccggtt ccttgggcgc cggtgtcagg
     1441 agagacgcca cgaggtagtt gacggcgaga acggcgagaa accacagcca gatcctgctc
     1501 ggggggagat tctttcccgg ctgacctcct ccggacgggc tgttcttccg caggaaaggc
     1561 tgaaatctgc cgctttccct tgatctgccg gtgtgctggg ttttcttttc catgaaaatc
     1621 ctcctcggtg cggatgatcc gccgtcccgg gatccgcggc agggaagagg cggcggtcca
     1681 ggaagaagcg ccgcctcttc tgatatggcc ggaatctatt atacgtcctt cccgaggttc
     1741 cctgtcaatc ccggtgcggg gatgcttgcc ccttccgtcg ggagattggg gaaaagaatg
     1801 ccaaaagtcc ccgtttggtg tattgttgga atgtcggaaa acgctaaaag gactgccgag
     1861 ggaggcatcg agagtgaaca gcgatttttt caacctgtac agccatgact tcatccgggt
     1921 ggcggcctgc gttcccgccg tccggaccgc ggacccttcc cataacgcgg agaggaccgt
     1981 cgaactgctc cgggaggcct cggaccgcag ggcggtgctc gccgtcttcc cggagcttgg
     2041 aatctccgcc tactcctgcg gggatctctt cttccaggac gccctgctgc agtccgctct
     2101 caagggactc gaaacgatcc tcggcgcttc ggcctccctg gacatggtca ttgccgcagg
     2161 agtcccgctg aagatagggg acgggctctt caactgcgct gccgtcctct tccggggccg
     2221 agtcctcggc gtggccgtca agagctttct ccccaactat cgggaatttt acgaactgag
     2281 gcatttcagg cccgcctccg cccttccgtc ggacaccctg gacctcctgg ggcagaagga
     2341 tatccctgtg ggggccgatc tcatcttcga ggcggaaggg attcccggct tcaggctttt
     2401 ctgcgaaatc tgcgaagacc tctggactcc ggtaccgccc tcctgctacg ctgccctctc
     2461 cggggccacg gtggcggtga acctgtccgc ctcgaatgtc accacgggaa aggcggacta
     2521 ccgccgggcc cttgtggcca accagtcggc ccggtgcatc gccgcctatg tctatgccgg
     2581 gac
//
LOCUS       DAOTHD010000235         2568 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_47804_length_2568_cov_3.848786, whole genome shotgun sequence.
ACCESSION   DAOTHD010000235 DAOTHD010000000
VERSION     DAOTHD010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47804_length_2568_cov_3.848786"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..786)
                     /locus_tag="P5219_05015"
     CDS             complement(139..786)
                     /locus_tag="P5219_05015"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRX26148.1"
                     /translation="MADFLRRELLVSAKYSQGTFIREEELARELNISRAPVREALKEL
                     EGQGLVRSIPRKGSMVLGFSPGDVEELYDIRFALETQVFEVLVKDGLLSGGDYERMHS
                     LFRELLAVASSSMEKNEKVLAFSRIDLDFHLFIAERSGRPWTIRILRGVYFQLHQAMV
                     QHLESEEELAYSAREHLKIIQSLQEGDLDALRESRHYSYFHRRLRKREKVEASGM"
     gene            994..>2568
                     /locus_tag="P5219_05020"
     CDS             994..>2568
                     /locus_tag="P5219_05020"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HRX26149.1"
                     /translation="MTKNDWFPNDDWFPGENSSPKRPFPSFPFRINKGLLAAGGAALL
                     LLVLFPALAEFYTDYLWYDTEGYGTIFWTRLFARVPLFGAGFLIYAVLLWLNLSAARR
                     NLRALYPEQEGLLGAKAAGFAIAAAALFLGVTNGAAMTGEWTMVLRYFHGTPFGEVDP
                     IFGRDIGFYVFGLPFWRFLHGKALNVVFLCLVTAGGAYAAFLLPRNRDLASLSVPPRM
                     RNHLALLASAGAFLWSAGYLLDRYNLLFSPTGVVFGAGYTDVHAELLALNVLAVLAAL
                     LGVSLLVSLLRKTWKFSLGVAGLVVVSSILLGGVYPAIVQKYFVEPNEFDREKQFIEY
                     NIEATLRAYGLKNITLRTVTPDDSITWENVEQNRDTINNIRLWDHSPLLRSYKQLQEI
                     RTYYNFSSVDIDRYTFGGQYRQVMLSPRELDLNGLQNPTWINTRLEFTHGYGIVMNPV
                     NEVAGSGMPRLWVRDLPPKTEVPLTIRRPEIYYGEKPTSYIFVGTTVKEFDYPMGESN
                     ARTTYEGKGGVPMGSLF"
BASE COUNT          530 a          809 c          657 g          572 t
ORIGIN      
        1 gctgctgtga acgtctttct gaacatagtg tcactcctcc tcaatatggg ttgcatcggt
       61 acaaacagaa caggaatctg cgccggcact gactgcgaat acacggaaaa cgccctgtgg
      121 catcactcct ttttctcctc acatgccgga agcttcaacc ttctcccgtt ttctcagcct
      181 tctgtggaag tagctgtagt gcctgctctc ccgcaaagca tcgagatctc cttcctgcag
      241 ggactggatg atcttcaaat gctcccgggc cgaataggca agctcctcct cgctttcaag
      301 gtgctgcacc attgcctggt gaagctgaaa atacacgccc ctgagaatcc ttatggtcca
      361 gggtctcccg gaacgttccg cgataaaaag atggaagtcg aggtcgatcc tgctgaaggc
      421 aagaactttc tcgtttttct ccatggaact cgacgccacg gcgagcagct cccgaaaaag
      481 gctgtgcatc cgttcataat ccccgccgga aagaagaccg tccttgacca gtacctcgaa
      541 cacctgggtc tcaagggcaa aacggatgtc gtaaagctcc tcgacgtccc ccggggaaaa
      601 gccgagaacc atggaccctt tcctggggat ggaccgtacg agtccctgtc cctcgagctc
      661 cttcagggcc tctctcaccg gggcccggct gatgttgagc tccctggcca gctcctcttc
      721 gcggataaag gtcccctggg agtatttggc tgaaacgaga agttcgcggc ggagaaaatc
      781 cgccacttcc ctcgcaaggg actgatggtt cgctatgctg tttatggaga gacctcctct
      841 gcaagtccta tcgtctactg tcgacagacg gttatatagg taccaccgca cggagagtct
      901 gtcaaggaaa ccacaattcg tataatgtat ccacaaaact tctccatcgt cttcattttg
      961 acatcccggc aaagtccttt agaatggatc attatgacta aaaacgactg gttcccgaac
     1021 gacgattggt ttcccgggga gaattcctcc ccgaagcgcc cgttcccctc ttttccgttc
     1081 cgtataaaca agggactcct ggcggcggga ggagccgctc ttctgctcct tgtgctgttt
     1141 cctgccctgg cggagtttta tacggattat ctctggtacg acaccgaagg atacggaacc
     1201 attttctgga cccggctctt tgcccgagtg ccccttttcg gggccgggtt tctgatctac
     1261 gccgtcctcc tgtggctgaa tctctccgca gccaggagga acctgagagc tctctacccc
     1321 gaacaggagg ggcttctcgg agcgaaggcg gcaggattcg ccattgcggc ggcggcgctg
     1381 ttcctcggcg tcaccaacgg agcggccatg accggggagt ggaccatggt tctccggtac
     1441 tttcacggca ctcccttcgg cgaggttgac cccattttcg gccgggacat cgggttctac
     1501 gttttcggcc ttcccttctg gagatttctc cacgggaaag ctctcaacgt tgtctttctg
     1561 tgccttgtga cggcaggcgg ggcatacgcc gcctttctcc tccccaggaa cagggatctg
     1621 gcgtccctat ccgtcccgcc gaggatgcgg aaccatcttg ccctcctcgc gtccgccggg
     1681 gcgttcctct ggtccgcagg ctacctgctg gacaggtaca acctcctctt ctcccccacc
     1741 ggagtggttt tcggagccgg atataccgat gtccacgccg aacttctcgc cctgaacgtc
     1801 ctcgccgttc tcgcggctct cctcggcgtc tcccttctcg tgagcctcct ccggaagacc
     1861 tggaaattct ccctcggagt cgccgggctc gttgtcgtct ccagcatcct gctcgggggc
     1921 gtctaccccg ccattgtcca gaaatatttc gttgagccca acgaattcga ccgggagaaa
     1981 cagttcatcg agtacaacat tgaagccacc ctcagggcct acggcctgaa gaacatcacc
     2041 ctgagaacgg tcacccctga cgattccatc acctgggaaa acgtggagca gaacagggac
     2101 accatcaaca acatcaggct ctgggatcac agccctcttc tcaggagcta caagcagctc
     2161 caggagataa ggacctacta taacttctcc agcgtggaca tcgacaggta cactttcggc
     2221 ggccagtacc gccaggtcat gctctctccc agggagcttg acctgaacgg ccttcagaac
     2281 ccgacctgga tcaacaccag gctcgagttt acccacggct acggcatcgt catgaatccg
     2341 gtgaacgagg tcgccggttc gggaatgccc cggctctggg tcagggacct ccccccgaaa
     2401 accgaagtcc ccctcaccat cagaagaccg gagatttact acggtgaaaa accgacctcc
     2461 tacatcttcg tgggcaccac ggtaaaggaa ttcgactatc ccatggggga atcaaacgcc
     2521 cgcaccacct atgaaggcaa ggggggcgtt cccatgggat ccctcttc
//
LOCUS       DAOTHD010000236         2565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_47886_length_2565_cov_2.480080, whole genome shotgun sequence.
ACCESSION   DAOTHD010000236 DAOTHD010000000
VERSION     DAOTHD010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47886_length_2565_cov_2.480080"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(272..865)
                     /locus_tag="P5219_05025"
     CDS             complement(272..865)
                     /locus_tag="P5219_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26150.1"
                     /translation="MFRPGFGSPAEIQRFLDSIPYNAKEECKPPERVFTEKNAHCFEG
                     ALFAAACLRELGYPPLVVDLKAWNDDDHVIAVFRERGCWGAVAKSNFTTLRFREPVYR
                     NLRELAMSYFDFYFNTLGQKTLRAYSRPLKLERYDGCRWADGDDDLEYIADGFNRLPY
                     YPLLGEEQIAGLSPVPDHLLQAGLAGSNPEGLYVPKE"
     gene            complement(926..2104)
                     /locus_tag="P5219_05030"
     CDS             complement(926..2104)
                     /locus_tag="P5219_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HRX26151.1"
                     /translation="MRCIGAVSRGIRLPVISRGADLVDIVSSELITASECPCDPFPLR
                     DRDIVGVTESLLARAQGNYATLEDIAEDVRRKVPEGDVALLFPILSRNRFGQILKGIL
                     RGVRGKVHILLSYPADEVGNLVMDPREFFLRSGRLKGGLFDEKEYEQVFGRYLHPFTG
                     VDYVRYYREMEPGRVEIHFADTPLAALEFSNTVIAASIHARHLHREILEQSGALVVSL
                     DQLCSEPHRVGMGYNPEFGLLGSNYTNDGTVKLFPRDSRPFALDLQKELQTRTGKRME
                     VLVYGDGAFKDPVCGIWELADPVVSPGYTDGLDGMPKEIKLKYVADNSGDKSPEEAVR
                     DAIRSKGAMDRFSHSTLGTTPRRLTDLIGSLCDLTSGSGDKGTPVIHISGYFDSYLDD
                     "
     gene            complement(2238..>2565)
                     /locus_tag="P5219_05035"
     CDS             complement(2238..>2565)
                     /locus_tag="P5219_05035"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HRX26152.1"
                     /translation="LPEDGEAVFVKHFPNSFRDTPLLDSLRELNPDRLVIAGMMTHMC
                     IDTTVRAAADLAFNCILAGDACATRSLAFGETAVPAEHVQAAFLAALSAFARVDTAAS
                     ICASLP"
BASE COUNT          547 a          760 c          770 g          488 t
ORIGIN      
        1 cacctatccg gatcggatcc gccgcaaaag cgggcagggc aaaaagacac accagggaca
       61 ccaacaccat cgctttccga atgctgccga aactcctcat tcttccaacc tcctcacatg
      121 atatggtgcc tttcggaaca gtatgactca atcaagttca ggcgaacgga atatccgccc
      181 gccggtcctc cggggaaaaa gaccaacatt gagaaaaaat tctccgcatg gcacaaataa
      241 taatactaca gccgaacctt ttccgcatca gtcattcctt cgggacatac agcccctcgg
      301 gattggatcc ggcaagaccg gcctgaagaa gatggtccgg gacaggcgaa agaccggcga
      361 tctgttcttc tccgagcagg gggtaatagg gcagcctgtt gaagccgtcg gcgatgtatt
      421 cgagatcgtc atctccgtcc gcccatctgc agccgtcgta ccgttccagc ttcagggggc
      481 gggaatacgc cctgagggtt ttctgcccaa gggtgttgaa atagaaatcg aaataggaca
      541 tggccagttc cctgaggttc cggtagaccg gctcccggaa ccggagagtg gtgaagttgg
      601 acttcgccac ggccccccag caccctctct cccggaagac agcgatcacg tggtcgtcgt
      661 cgttccaggc tttgaggtcc accaccaggg gagggtatcc gagctcccgg aggcatgccg
      721 ccgcaaagag ggcgccctcg aaacagtggg cgttcttttc ggtgaaaacc ctttccggcg
      781 gtttgcactc ctctttcgca ttgtagggaa tggagtcgag aaaccgctgt atttccgcag
      841 gacttccaaa gcctggccgg aacatgaaac caccctccgg aataaaaatg gccggaaggc
      901 ataatccctt ccggcattgt ttccatcaat cgtccaggta agaatcgaag tagcccgaaa
      961 tgtggatcac cggggttccc ttgtctcccg acccggacgt gagatcgcag agggatccga
     1021 taaggtcggt gagcctccgg ggggtcgtcc cgagagtgga atggctgaac cggtccatgg
     1081 cgcccttcga gcggatggcg tcccgtacgg cctcttccgg agacttgtcc ccggagttgt
     1141 cggcgacgta cttgagcttg atctccttcg gcattccgtc gaggccgtcg gtgtaaccgg
     1201 gtgagaccac cgggtcggcc agttcccaga tgccgcagac ggggtccttg aaagctccgt
     1261 cgccgtagac gaggacttcc atgcgttttc ccgtccgggt ctgaagctcc ttctggagat
     1321 ccagggcaaa ggggcggctg tcccggggaa agagcttcac tgtcccgtcg ttcgtgtaat
     1381 tcgatcccag aagcccgaat tccgggttgt atcccatgcc caccctgtgg ggctccgaac
     1441 agagctggtc gagggatacc accagggccc ccgactgctc cagtatctcc ctgtgaagat
     1501 gccgggcatg gatgctcgcc gcgatgaccg tgttcgagaa ttccagggcc gccagcgggg
     1561 tatcggcgaa atggatctca accctgccgg gctccatctc cctgtaatac ctgacgtaat
     1621 ccacgccggt aaaggggtgg aggtaacgcc cgaagacctg ctcgtactcc ttttcgtcaa
     1681 aaaggccgcc tttcagcctg ccgctcctga ggaaaaactc ccggggatcc atgacaagat
     1741 tgcccacctc gtcggccggg taggaaagaa gaatgtggac cttcccccgg acgccccgca
     1801 ggatgccttt cagtatctgc ccgaaccggt tcctgctgag gatcgggaac agaagggcca
     1861 cgtcgccctc cggaaccttg cggcggacat cctccgctat gtcctccagg gtggcgtaat
     1921 tgccctgggc cctcgccagg agagattccg tcacgccgac gatgtcgcgg tcacggaggg
     1981 gaaaggggtc gcaggggcac tccgatgctg taatcaattc cgaggaaacg atatcaacga
     2041 ggtctgcacc ccgggaaatg acggggagac gaattccccg cgaaacggcg ccgatgcagc
     2101 gcatgggtcc tcattccttc cgaagagggt gtgtggatgg ctgcaggaac ggcgtcccgc
     2161 gcctccgata ttgtatcatt tttccgccac ccgacaaaag gaggaattac gccgggaaaa
     2221 ggatcgttcc cccgcgttta gggaagagag gcacagatcg aagcggccgt gtcgaccctg
     2281 gcgaaagcgc tgagagccgc gaggaaggcc gcctggacat gctccgcggg aacggcagtc
     2341 tctccgaagg caagggatcg tgtcgcgcag gcatccccgg caaggatgca gttgaaggcc
     2401 aggtcagcgg cagcccggac cgtggtgtcg atgcacatgt gggtcatcat gcccgcgatg
     2461 accagccggt cgggattcag ttcccgcaga ctgtcgagga gcggagtgtc ccggaagctg
     2521 ttggggaaat gcttgacgaa aacagcctct ccgtcttcag ggagg
//
LOCUS       DAOTHD010000237         2557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_48080_length_2557_cov_3.878497, whole genome shotgun sequence.
ACCESSION   DAOTHD010000237 DAOTHD010000000
VERSION     DAOTHD010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48080_length_2557_cov_3.878497"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..>2557)
                     /gene="gyrA"
                     /locus_tag="P5219_05040"
     CDS             complement(171..>2557)
                     /gene="gyrA"
                     /locus_tag="P5219_05040"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRX26153.1"
                     /translation="FLDYSMSVIVSRALPDVRDGLKPVHRRILYAMHGLNLSKGRPYR
                     KSAAIVGDTMGKYHPHGDSAIYDAMVRMAQDFSMRYPLVNGQGNFGSIDGDPAAAMRY
                     TEAKLYEMGELMLADIEEDTVEWGPNFDESLKEPLYLPSLVPNLLVNGSSGIAVGMAT
                     NIPPHNLSEVVDALEYMIDSDGNWEFGEIYARLPGPDFPTGGIILGRDGIIDAYRTGR
                     GKVILRGKTVVEDGKRGKTSVIITEIPFMVNKTSLIETIASCVQEKHIDGVADIRDES
                     DRDGLRIVLELQRDGDADLVLRQLYRRTQLQTTFGVINLALVNQHPEELPIADMLSHF
                     LEHRRDVVRRRTQFRLDKALARAHIVEGLVKALDMIDRVIALIRGARTVQEAKDGLIS
                     QLGFSETQAQAILDMRLQRLTGLEREKLEAELAALFADIEGYRTILGNRSALDGVIKS
                     ELAELKRKFGGPRRTEIMDNYEEVSMEDLIPENDIVVILSKDGYLRRKALEEYSLQAR
                     GGKGKKGSGLQDEDEIALVAVTNTHKDIYLFTSSGRALAIRGHMIPESRTGKGKLIGK
                     LVPLDEGETVVSMYGRSLEGKSFIFFATRKGISKRLPLSEIARLNRAGKRVLTLDDGD
                     AIAQVRLTTGKDELLFMTANGQGLRVSEEEFRPMGRSARGVKGIRLEEGDSVISCEVV
                     TSDRLAMVISERGVAKRTSFEEFSIHHRGGKGVKAMNLGRKTGPLVGSWAVAEDDEIM
                     VITSRGRMIRVAVVEIPRLSRTAMGTITVRLDDGDSVADVSVVCGDECVEENRS"
BASE COUNT          470 a          772 c          763 g          552 t
ORIGIN      
        1 gggagagaga aatccgggcc cgtcgggaac cctttccgga tcccggaaaa accagaccac
       61 cagcccgagc aggggagcca ggaacaggcc taccatgggg gcgaagtaca ggctgccgag
      121 cagcatgaag gcgacaaaag ctatcagggg atatccgtcc cgtacgattt tcacgatctg
      181 ttctcctcca cgcattcatc tccgcagacc acgctcacgt ccgccacgga atcaccgtcg
      241 tcaagccgca cggtgatcgt tcccatggca gttctgctca gccgcggtat ctccaccacg
      301 gcgacccgga tcatcctgcc cctcgaggtg atgaccatga tctcgtcatc ctctgcgaca
      361 gcccagctcc cgacgagcgg accggtcttc ctgcccaggt tcatggcctt gacgcccttg
      421 ccgccgcggt gatggatgga aaattcctcg aagctcgtcc tcttggcgac accccgctcg
      481 ctgatcacca tggcgagacg gtcggatgtg acgacttcac agctgatgac agaatcccct
      541 tcctcaaggc ggataccctt cacgccccgg gcagaacggc ccatggggcg gaattcttcc
      601 tcggagacgc ggaggccctg gccgttcgcc gtcatgaaga gaagttcgtc ctttccggta
      661 gtcagcctga cctgagcgat tgcatctcca tcatcaaggg tcaggaccct tttccctgca
      721 cggttgagcc tggctatttc cgaaagggga agccgcttgg agatgccttt cctcgtggca
      781 aagaaaatga agctttttcc ttcgaggctc ctgccgtaca tggagaccac cgtctctccc
      841 tcgtcgaggg gaaccagctt tccgatgagc tttccctttc cggtcctgga ctcggggatc
      901 atgtggcccc ggatggcgag ggcccgcccg gaggaggtaa agaggtagat atccttgtgg
      961 gtgttggtga ccgccacgag agcgatctcg tcctcgtcct gcagcccgct tccttttttg
     1021 cccttgcctc ccctggcctg gagggaatat tcctcgaggg ccttccggcg gaggtagccg
     1081 tccttggaga ggatgacaac gatgtcgttc tcagggatga ggtcttccat ggacacttcc
     1141 tcgtaattgt ccatgatctc agtcctccgg gggccgccga atttccgctt cagctcagcg
     1201 agttccgact tgatgacacc gtcgagggcg gaacggtttc cgagaatggt ccggtacccc
     1261 tcgatgtcgg cgaagagcgc cgcaagttcc gcctcgagct tttcccgctc gagcccggtg
     1321 agccgctgga gcctcatgtc aaggatagcc tgggcctggg tctccgaaaa tccgagctgg
     1381 gaaatcaggc cgtccttcgc ctcctgaacg gtccgggcac cccggataag cgcgatgacc
     1441 cggtcgatca tgtccagggc cttcacaagt ccttccacga tgtgggcccg ggcgagggct
     1501 ttgtccagcc tgaactgggt tctcctccgg accacgtctc tccggtgttc gaggaagtgg
     1561 gagagcatat ccgcaatggg aagctcttca ggatgctggt tcaccagggc gaggttgatg
     1621 acgccgaaag tggtctggag ctgggttctc ctgtagagct gccggagaac gagatcggcg
     1681 tcgccgtccc gttggagttc cagaacgatg cgaagtccgt cgcggtcgga ctcgtcccgg
     1741 atgtccgcca cgccgtcgat atgtttttcc tggacgcagg aggcgatggt ttcgatgagg
     1801 gacgtcttgt tcaccatgaa gggaatttcc gtgattatca cggacgtctt gccccgcttc
     1861 ccgtcctcca cgacggtctt tccccgaagg atgacctttc ccctgcccgt ccggtatgca
     1921 tcaatgatcc cgtccctgcc gagaatgatg cctcccgtcg ggaagtcagg cccgggaagc
     1981 cgggcgtaga tttcgccgaa ttcccagttt ccgtctgagt cgatcatata ttcaagggca
     2041 tcgacaacct cggaaagatt gtgaggggga atgttcgtgg ccattcccac cgcaattccc
     2101 gagcttccgt tcacgaggag gttggggacg agggagggaa ggtagagggg ttccttcagg
     2161 gattcgtcga agttgggtcc ccactccacc gtatcctctt cgatgtcggc aagcatgagc
     2221 tctcccattt cgtagagctt cgcctcggtg tacctcatgg ccgccgcggg atctccgtcg
     2281 atggagccga agtttccctg gccgttcacc agggggtatc tcatgctgaa atcctgggcc
     2341 atgcggacca tggcgtcgta aatggcggag tcgccgtggg gatggtattt tcccatggta
     2401 tcgccgacaa tggccgccga ttttcgatag gggcgcccct tgctgagatt gagcccgtgc
     2461 atggcgtata gaatgcgccg gtgcaccggt ttcaatccgt ctcgaacgtc gggcagggcg
     2521 cggcttacga tgacgctcat ggaatagtcg agaaagg
//
LOCUS       DAOTHD010000238         2555 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_48133_length_2555_cov_3.333600, whole genome shotgun sequence.
ACCESSION   DAOTHD010000238 DAOTHD010000000
VERSION     DAOTHD010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48133_length_2555_cov_3.333600"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="P5219_05045"
     CDS             <1..210
                     /locus_tag="P5219_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HRX26154.1"
                     /translation="IDENGWPAHKRTARHVTTMMTILQVHNMLTPDKAIILDGIPSYG
                     DLMDNGMGQYFADPDASPAERVFFD"
     gene            complement(262..1503)
                     /locus_tag="P5219_05050"
     CDS             complement(262..1503)
                     /locus_tag="P5219_05050"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRX26155.1"
                     /translation="MNPREMYLQQLEQAVPYLTVEPEYLDMLRYPKEVLELSLPVRLD
                     GGGVKIFSAWRSHHNNALGPYKGGVRYHVEVSRDEVVALSSWMTIKCSIAGLPYGGGK
                     GGVAVDVRELSRNELENLSRAYAFGISRFVGTDYDIPAPDVYTNPQVMAWFLDTFEKV
                     KGWSEPSAFTGKPVVMGGSLGRSDSTGKGGIFILREALKAMGLLGKPLKVGIQGYGNV
                     GSRAHRFVEEDLGLTVVAVCDSAAAIYNPSGLKYSEVGKYKEETRSLKGFPGGEEFFP
                     DLIFEKEMDILILAALGGAIDEKNAGGVKAKVILELANGPITPEGEAVLSDKGILIVP
                     DVLANAGGVIVSCFEWIQGRSGDMWSEEYVFSKLDERMVRSFGEIWRIREDRKISFRQ
                     AAYVKAVSRIVDAMRFRGIWP"
     gene            complement(1695..>2555)
                     /locus_tag="P5219_05055"
     CDS             complement(1695..>2555)
                     /locus_tag="P5219_05055"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRX26156.1"
                     /translation="VIGAWTDVPAPDVNTGGPEMVWFMDTISKMKNQLEPAIFTGKPI
                     PLWGSKGRTAATGYGVATCALELLKNLGKSPEGATVAIQGFGNVGTYAALTMIDSGSK
                     VIGISDITGTYYAPNGLDIKKAFDHVSNHPKRLLEGFTCDGCQKLDLPEVLFLDCDIL
                     IPAALEGVINGKNADKVKAKYIVEAANGPITPEGDAILDEKGILVVPDFLANSGGVIG
                     SYFEWAQDLGGFFWSEEEYNDRLIRIMKDNFQRVWTYAKDKNVKMRRAAFMAAIQRVA
                     DAVRMRGVFL"
BASE COUNT          560 a          747 c          665 g          583 t
ORIGIN      
        1 atcgatgaaa acgggtggcc tgcccataaa aggacagccc ggcatgtcac gacgatgatg
       61 acgattcttc aggtccacaa tatgctgacc cccgacaagg caatcattct cgatggaatt
      121 ccctcatacg gagacttgat ggacaacggc atggggcaat actttgccga ccctgacgca
      181 agccctgccg aaagagtgtt tttcgactga ctgtcatgcg ggggaaggac cttccggcct
      241 cttcccccgc tctatacccc atcagggcca gataccccgg aaccgcatgg catccacgat
      301 cctgctcact gccttcacgt aagctgcctg ccggaagcta atcttccggt cctcccgtat
      361 cctccagatc tcaccgaagg agcggaccat ccgttcatca agcttggaaa aaacatattc
      421 ctcggaccac atgtctccag agcgcccctg gatccattca aaacagctga cgatgactcc
      481 tccggcgttg gcaaggacat cgggcacgat aaggattccc ttgtcggaga gaacggcttc
      541 gccttcagga gtgatgggac cgttggccag ctcaagaatc acctttgcct ttactcctcc
      601 ggcattcttt tcgtctattg ccccgccgag ggcagccagg atgagaatat ccatttcctt
      661 ttcaaaaatc aggtcaggga agaattcttc tcctcccgga aaacctttca atgagcgggt
      721 ttcttccttg tatttaccga cttccgaata tttcaatccc gagggattgt aaatcgcagc
      781 ggcggaatcg cagaccgcta cgacagtaag accgagatcc tcctcaacga accggtgggc
      841 tctcgatcca acgttgccgt acccctggat cccgaccttc agtggttttc cgagtaatcc
      901 cattgccttg agggcttccc ggaggatgaa gataccgccc tttcccgtcg agtccgacct
      961 cccgagggag ccgcccatga ccacaggctt tccggtaaaa gcggagggtt cgctccatcc
     1021 tttcactttc tcgaaggtat caaggaacca ggccattacc tggggattgg tgtacacatc
     1081 gggggcggga atatcgtagt cggtcccgac gaaccgggat ataccgaacg cataggctct
     1141 gctgagattc tccagctcat tccgtgagag ttccctgaca tctacggcaa cgcctccttt
     1201 cccgccgccg taggggagtc cggcaatgga acacttgatg gtcatccagg aggaaagggc
     1261 aaccacctcg tccctgctca cctccacgtg gtaccgaacc ccacccttgt agggaccgag
     1321 agcgttgttg tgatgactcc gccacgcact gaaaatcttc acgcctcccc cgtcgagccg
     1381 cacgggaaga gaaagctcaa gcacttcttt cgggtacctg agcatatcaa ggtattccgg
     1441 ctccacggta aggtaaggca ccgcctgttc aagctgctga agatacatct ctctcgggtt
     1501 catccgttca tccacctcct cggagatcta cttccccatg gaaaacagag accgatgcgc
     1561 cactgacctc tttgaaaatt ctaacataaa cagtgctttt tttgactact tatttgtctt
     1621 ttctgtctaa ttaagagagc aaaaaaagag gccggatcac tccggcctct ttacatcagc
     1681 ttcaatattc aggtttacag gaacacgcct ctcatccgga ccgcgtcggc caccctctgg
     1741 atggccgcca taaaggctgc ccggcgcatc ttgacgtttt tgtccttcgc gtacgtccaa
     1801 accctctgga agttgtcctt cataatgcgg atcagccggt cgttgtactc ctcctccgac
     1861 cagaaaaatc cgccgaggtc ctgggcccac tcgaagtagg agccgatgac tccgccggaa
     1921 ttggcgagga agtcggggac gacgagaatg cccttttcgt cgagaatggc gtcaccttca
     1981 ggagtgatgg ggccgttggc tgcttcgaca atgtacttcg ccttgacctt gtcggcattc
     2041 tttccgttga tgacgccctc aagggcggcg gggataagga tgtcacaatc caggaaaaga
     2101 acttcgggaa ggtcgagctt ctggcagccg tcgcaggtga agccttcaag aaggcgcttc
     2161 gggtggttgc tcacgtgatc gaaggctttt ttgatatcaa ggccgttggg agcatagtat
     2221 gttcctgtga tatcgctgat tccgataacc ttggacccgg aatcgatcat tgtaagggcc
     2281 gcgtaagtgc ccacgttgcc gaaaccctgg atggcgacgg tggctccttc cggagacttg
     2341 ccgaggtttt tgagaagctc aagggcgcag gttgcgacgc cgtagccggt cgcagcagta
     2401 cggcccttgg atccccacag gggaatgggc ttgccggtaa aaatggccgg ctcgagctgg
     2461 ttcttcatct tgctgatggt gtccatgaac cagaccattt caggtccgcc ggtgttgacg
     2521 tccggtgcgg gaacgtcggt ccaggcgccg atgac
//
LOCUS       DAOTHD010000239         2550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_48250_length_2550_cov_2.693387, whole genome shotgun sequence.
ACCESSION   DAOTHD010000239 DAOTHD010000000
VERSION     DAOTHD010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48250_length_2550_cov_2.693387"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /gene="deoC"
                     /locus_tag="P5219_05060"
     CDS             <1..619
                     /gene="deoC"
                     /locus_tag="P5219_05060"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRX26157.1"
                     /translation="KPEARGEDIRRLCEEAKEYGFASVCVNGSFVETAVRELNGSGVK
                     VCAVVGFPLGACSSETKAFEAREAVALGAEEIDMVLSVGRLLSGNEEYVREDVRKVVE
                     AAEKGALVKVILETCLLDDEKKVLGCRLAEEGGARFVKTSTGFSSGGATAEDVALMRR
                     TVGNRLGVKASGGIRTRRDAEAMIAAGASRIGASASVAICSEDHS"
     gene            637..1647
                     /locus_tag="P5219_05065"
     CDS             637..1647
                     /locus_tag="P5219_05065"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRX26158.1"
                     /translation="MVFCSAPDSIPIRILVTGSRGKSSLVRLLTAALEGAGVSAAGRI
                     TGVLPREIFRGKETLILRSGPGSVQEMRWWLRSLPGGTEGAVLENSAVDPELQGLAWR
                     WMKPSCTVLTNVRPDHEEVWGRGEEAAAAALCGGIGGGTVILPCSVASVPAVPPILGK
                     KGCGLLPCPDGACYRETHLSLVSGVCSFLGLDSGKALAAAREVAPDIADFRLIREGRG
                     ILASAFSANEPESTETLFQSTGWERQETTVLYNSRKDRVARLRSFLPWLSEHSWKRVA
                     VTGSRPLFLPSGAAFLSLRDPGELRAFVASEGKVFGCGNVAGVPLQYLLEREEGEERF
                     LD"
     gene            1640..2080
                     /locus_tag="P5219_05070"
     CDS             1640..2080
                     /locus_tag="P5219_05070"
                     /inference="COORDINATES: protein motif:HMM:NF025467.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein
                     PgsC/CapC"
                     /protein_id="HRX26159.1"
                     /translation="MTDGDVLLVALGIALGLFLFHRTGYSPGGIITPGFLAMELASPA
                     RVAAAFGCAVIVGALLSLLVRGTGLYGRQRTGAAMLLALGVKVVLGDLFPAAPAWIGW
                     VIPGLIGADMQRQGIVPTAAASLASAFAASLAAALLVSLSGVSP"
     gene            2077..>2550
                     /gene="pgsW"
                     /locus_tag="P5219_05075"
     CDS             2077..>2550
                     /gene="pgsW"
                     /locus_tag="P5219_05075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HRX26160.1"
                     /translation="MKKSLLEVQRIFLRNARLRLVFLAAVLGAAWYFGTVDRVSGDEL
                     RLWEKVRTAQDHLFRWREDRGVAPEEGSDREKTGFIGVEWSPLTTTLGPLEAKRTAAD
                     PLWAVHALRSFRALGIGEGDNVALLSSSSFPGLVYSILAAAEHLGADVLWIHSLGA"
BASE COUNT          405 a          758 c          865 g          522 t
ORIGIN      
        1 gaaacctgaa gcccggggtg aggacatcag gcgtctctgt gaagaggcga aggagtacgg
       61 cttcgcctcg gtgtgcgtca acggatcctt cgtggagacg gccgtcaggg agctgaatgg
      121 ctccggggtg aaggtctgtg cggtggtcgg attccccctc ggcgcctgct cgagcgaaac
      181 gaaggccttt gaggcccggg aagccgttgc cctgggggcg gaggagatcg acatggtcct
      241 ttccgtggga cgtcttcttt ccggtaatga ggagtatgta cgggaggacg tccggaaggt
      301 ggtggaagcc gccgaaaaag gcgccctcgt caaggtcatt ctcgagacct gcctcctgga
      361 cgatgaaaag aaggttctcg gctgtcggct ggcggaggaa gggggagccc gtttcgtcaa
      421 gacgtccacg ggtttttcct ccggaggcgc caccgctgaa gacgtggcgc tcatgcgccg
      481 cacagtgggc aaccgcctcg gcgtcaaggc gtcgggaggg atccgcacac gaagggatgc
      541 ggaggccatg atagccgcgg gggcgagccg cataggcgcc tcggcgtctg tggccatctg
      601 ttcggaagat cactcctgag ggcgggttta gggaccatgg tattctgcag tgctcctgac
      661 agcatcccca ttcggattct tgtcacgggg agcaggggaa aaagctccct tgtccgcctc
      721 ctgacggccg ctctcgaggg ggcgggagta tcggcagccg ggagaattac cggggttctt
      781 ccccgggaga tcttccgggg aaaagagacg ctcattctcc gctccggtcc cggcagcgtg
      841 caggaaatgc ggtggtggct ccgctccctg cccggcggaa cggagggagc ggtgctcgaa
      901 aacagcgccg ttgatccgga gctgcagggg ctcgcctggc ggtggatgaa gccgtcctgt
      961 acggtgctca ccaatgtccg tcccgaccac gaggaagtct ggggccgcgg agaagaggcg
     1021 gccgcggcgg ctctctgcgg aggaataggc ggagggacgg tgatcctccc ctgttccgtt
     1081 gcatctgtcc cggcagtacc ccccattctc gggaaaaagg ggtgcgggct tttgccctgt
     1141 cccgacggcg cctgttacag ggaaacccat ctttccctcg tttccggagt ctgctctttt
     1201 ctgggccttg acagcgggaa agccctggcg gccgcccggg aggtcgcccc cgacatagct
     1261 gatttccgcc tcatccggga agggagaggg attcttgcct cggccttttc cgccaacgag
     1321 cccgaatcca cggaaaccct gttccagtcc accgggtggg agagacagga gaccacggtg
     1381 ctttacaact cgagaaagga tcgtgtcgct cgtctgcgct cctttctgcc ctggctgtct
     1441 gaacattcct ggaagcgcgt ggctgtcact ggaagccggc ctctttttct gccttccggc
     1501 gccgcatttc tttcccttcg ggaccccgga gagctgaggg ccttcgtcgc ttcggaagga
     1561 aaggtcttcg gatgcgggaa cgtggccggc gtccccctgc aatatttgct cgaacgggag
     1621 gaaggggagg aaagattcct tgactgacgg agatgtgctc cttgttgccc tcggcattgc
     1681 cctggggctg ttcctttttc accggaccgg gtattccccc ggcggcatca tcactcccgg
     1741 cttcctggcc atggaactgg cctcgcccgc tcgggtcgcg gcagcgttcg ggtgtgccgt
     1801 gattgtaggc gctctccttt cccttctcgt caggggaacg ggcttgtacg gccgccagag
     1861 aaccggtgcc gccatgctcc tcgctcttgg agtgaaggtg gtgctggggg acctcttccc
     1921 cgccgctcct gcctggatcg ggtgggtcat tccggggctt ataggagccg acatgcagcg
     1981 tcaggggatc gtccccacgg ctgctgcatc ccttgcctcg gccttcgccg cctccctggc
     2041 ggccgccctg ctggtttccc tttccggagt gtcgccatga aaaagtccct tctcgaggtg
     2101 cagcgaattt ttttgcggaa tgccaggctg aggctggttt ttcttgcagc cgtcctcggt
     2161 gcggcctggt atttcggcac cgtcgacagg gtgtcgggcg acgagctcag gctctgggag
     2221 aaagtccgga ctgcccagga tcatcttttc cggtggaggg aggaccgggg cgttgccccg
     2281 gaagagggaa gcgacaggga gaaaacggga ttcatcggtg tggaatggag tcctctcaca
     2341 accaccctgg gtccgctgga ggcgaaacgg accgccgcag accccctctg ggccgtccat
     2401 gcccttcgga gcttccgggc cctgggcatc ggagagggcg acaatgtggc gcttttgtcg
     2461 tcgtcatcgt tcccgggcct tgtctactca atcctggcag cggcggaaca tctgggcgcg
     2521 gatgtgctgt ggattcattc cctcggcgcc
//
LOCUS       DAOTHD010000240         2546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_48355_length_2546_cov_3.195504, whole genome shotgun sequence.
ACCESSION   DAOTHD010000240 DAOTHD010000000
VERSION     DAOTHD010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48355_length_2546_cov_3.195504"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..1007)
                     /locus_tag="P5219_05080"
     CDS             complement(81..1007)
                     /locus_tag="P5219_05080"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HRX26161.1"
                     /translation="MFRKCTLACIFFVVLCGFSGSAWAKPRIAILEFEDKSGAGAPGE
                     AVADMLTTELFNTNEFTILERQRMDAILSEQDLSSEGYVDSSTAVKMGKLLGVDFLVT
                     GAITQFKTETAGGVVPLPLGSFGGVAVGSHTAYVTLDVRAVSTTTGEILLAAREQGAA
                     NATVGGVAAYGAAFGGGKTGGVLASATYKVVTKIVPRISSLRDKAAEAALDAVFNVIE
                     GGNVMVAVDAGASSGIKVGQYLAAFKEGKVLKDMQGNVLDAEKIYTAVLVVREVKPKY
                     SKCEVIRALKPLSRGEKAEFLKGSPEDLPIGK"
     gene            1215..1658
                     /locus_tag="P5219_05085"
     CDS             1215..1658
                     /locus_tag="P5219_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRX26162.1"
                     /translation="MKLSTKTRYGLRALLYMAERYDGERAVPVGEIAKEQQVSEKYLE
                     QLFIKLRRKNIICSVRGAQGGYMLRKHPREVTVASVVEALEGDITFADCLSSGGCRNK
                     GSCATHDLWSRLKDSIDGILEDTTLFDLVNKSGSGENNAAGVANG"
     gene            1683..>2546
                     /locus_tag="P5219_05090"
     CDS             1683..>2546
                     /locus_tag="P5219_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRX26163.1"
                     /translation="MSRQVYLDYAATTFTAPEVVSAMTPYFTESFGNPSSIYSFSERN
                     RKAIDAARQKVADVLGATKEEIYFTSGGTEADNWALKGAAWRNRSKGNHVITTKIEHH
                     AVLHTAQFLQENGFDVTFLDVDAEGRISLDDLKKAVTGKTVLVSVMFANNEIGTLQPV
                     AEIGAFCREKGILFHTDAVQATAHVPIDVKAMNIDLLSLSAHKFYGPKGVGALYVRKG
                     VRIDNFMHGGGQERGRRATTENVAGIVGLGAAIELTSGRMKTASARIGGLRDLLVEGV
                     MKTIPHAKLNGA"
BASE COUNT          553 a          746 c          723 g          524 t
ORIGIN      
        1 gggcctttcc tgaagttttt ttgccgtcgt tcaaagagcc cgcagggttt tccctgcggg
       61 ctctttgtcg gcttttcccc ttatttccct atggggagat cttctgggct acccttcaga
      121 aattcggcct tttcaccccg ggagagtggc ttcagagccc ggatgacctc gcacttcgaa
      181 tacttcggct tcacttccct caccacgagc accgccgtgt agatcttctc ggcatcgagg
      241 acgtttccct gcatgtcctt gagcactttg ccttccttaa aagcggcgag gtactggccg
      301 accttgatgc cggacgacgc tcccgcgtcc acggccacca tcacgttgcc cccttcgatg
      361 acgttgaaga cggcatccaa cgccgcttcg gccgccttgt ccctgaggga ggagatacgg
      421 ggaacgattt tcgtcaccac cttgtaggtt gccgaggcca gcacgcctcc tgtctttcct
      481 cctccgaatg ccgcaccgta ggctgcaacg cctcccaccg tggcgttggc agctccctgc
      541 tcccgggcgg cgagcagaat ttctcccgtg gtggtgctga ctgcccggac atcaagggtg
      601 acataggccg tatgacttcc cacggccact cctccgaagc tccccagggg caggggcacc
      661 actcctccgg cagtttccgt cttgaactgg gttatggctc ccgtaacgag gaagtccacg
      721 ccgagcagtt ttcccatctt gacggccgtt gagctgtcca catatccttc cgaagaaaga
      781 tcctgttccg aaaggatggc atccatgcgc tgccgttcga ggatggtaaa ttcattggtg
      841 ttgaagagct ccgtggtgag catgtcggcc acggcttcac caggggcgcc ggcaccgctc
      901 ttgtcctcga actcgaggat ggcgattctc ggttttgccc atgctgatcc tgagaagccg
      961 cacaggacca caaaaaaaat gcaggcgagg gtacactttc tgaacatggc gaacactccc
     1021 gtctcacgtg aaatttttga acaaactgcc ccgttgcacg gaaacaatag tatcccgtgg
     1081 ccctctcctt ttcaacggtt ttcctccaaa tcttcaggta tctggttgaa ggaaaaatca
     1141 agattaatgt tgacaagaat ggtaggatat agtatctttt ctccggacca tacaggcagg
     1201 ggatgtgata ggaaatgaaa ctctccacca agacgagata cggcctcagg gctcttctgt
     1261 acatggccga acggtacgac ggtgaaaggg ccgttcccgt gggagaaatc gcgaaggaac
     1321 agcaggtttc ggagaaatac ctggaacagc ttttcatcaa gcttcggcgc aagaacatca
     1381 tttgcagcgt ccggggagcc cagggaggct acatgctgag aaagcacccc cgggaggtga
     1441 ccgtagcttc cgtggtcgag gctctcgagg gggacatcac ctttgccgac tgtctcagct
     1501 ccgggggatg cagaaacaag gggtcctgcg ccacccatga tctctggagc aggctcaagg
     1561 acagcatcga cggaattctt gaggatacca ccctttttga ccttgtgaac aaatccggga
     1621 gcggggaaaa caacgccgcc ggcgtggcga acggctgaag ccgaagaaag gagcaaccca
     1681 gaatgtcaag gcaggtttat ctcgattatg ccgcaacaac ctttacagcg cccgaagtgg
     1741 tttccgcaat gactccctac tttacagagt ccttcggcaa cccgtcctca atttattcct
     1801 tttccgaaag gaacaggaag gccatcgacg ccgcccggca gaaggtcgcg gacgtgctgg
     1861 gcgcaacaaa ggaagaaatc tattttacca gcggcgggac cgaggcggac aactgggctc
     1921 tgaaaggcgc ggcgtggaga aaccgatcaa agggcaacca tgtgatcacc acgaagatag
     1981 agcatcatgc cgtgctccac actgcccagt tcctccagga gaacggcttt gacgtgacct
     2041 ttctcgatgt ggacgccgaa ggccgcatca gtctcgacga cctgaagaag gccgtgacgg
     2101 gcaaaaccgt tctcgtgtcg gtgatgttcg ccaacaacga gatagggacc ctgcagcccg
     2161 tggcggagat cggcgccttc tgccgggaaa aggggatcct gttccatacc gacgcggtcc
     2221 aggccacggc ccatgtcccc atcgacgtga aggccatgaa catcgatctt ctgtcccttt
     2281 ctgcccataa attctacggc cccaagggtg tcggcgccct ctacgtgcgg aagggcgtca
     2341 ggatcgacaa cttcatgcac ggcgggggac aggaaagagg gcggcgggcc accacggaga
     2401 atgttgccgg tatcgtcgga ctgggcgccg ccatcgagct gacgtccggc aggatgaaga
     2461 cggcctctgc gcggatcggc ggactgcggg atctgctcgt ggagggtgtc atgaagacca
     2521 tcccccacgc gaaactgaac ggggcc
//
LOCUS       DAOTHD010000241         2544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_48417_length_2544_cov_2.504219, whole genome shotgun sequence.
ACCESSION   DAOTHD010000241 DAOTHD010000000
VERSION     DAOTHD010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48417_length_2544_cov_2.504219"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1418)
                     /gene="pruA"
                     /locus_tag="P5219_05095"
     CDS             complement(<1..1418)
                     /gene="pruA"
                     /locus_tag="P5219_05095"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR01236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HRX26164.1"
                     /translation="MNNSFFMFREPENEPLLGFAPGTPEKRSLKAALAKVRSEVPEIP
                     LIIGGREYGSEEKGRVVMPHDHGHVLATHCKASPADVERAVKEAARAKKEWENTPWTE
                     RAAVMLKIAELFTTEYRDLLLASTMLGQSKNVFQAQIDTVAEAADFIRFNVAYASSIY
                     AEQPRLDRDSLNRMEYRPLEGFVFAVTPFNFTAIASNLVLAPVLMGNTVLWKPAGTSL
                     LSSYYLMKIYEKAGLPAGVVNFLPGSGEMISRVVTKHPDLAGIHFTGSTDVFSKLWMQ
                     CAENLRNYRSFPRLVGETGGKNFLVVHPTADVDAAVTAMVRGAFEYQGQKCSAVSRCY
                     IPKSLREKILSLAGRMIADMKTGSPEDFRNFINAVIDEASFDNCMKYISAARMSTEAD
                     ILFGGRGDKSAGYFVEPTVIETTDPEFVTMREEIFGPVLTLYTYEDDRFGEILELCDR
                     SSPYGLTGAVFAADRKALALAAR"
     gene            1754..>2544
                     /locus_tag="P5219_05100"
     CDS             1754..>2544
                     /locus_tag="P5219_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HRX26165.1"
                     /translation="MGILSADKGAYSRKVDLFFRHPVVEGALLFLISASVLAAIASIL
                     FDHLFLEWLNLFFSALFSVELTARYFTYGGKEKDYVRDWWMDWAATIPWDVFLSFLFP
                     GGGASMLRLLRLPRIFRLLRFRRLKKSEAARWLSYRFRRLMEVSIWRQSLTLLLVSLV
                     FVWVFTVLLDRVGMNSEHGGNFWFSLITMISSDSIFEMQGQDSLVKGLVLLLSFIGIV
                     LFNGILIAIIIGKLMERLGEMKDAGEVRERGHILLLGWSECIPHIL"
BASE COUNT          466 a          760 c          725 g          593 t
ORIGIN      
        1 cgggcggcaa gagccagggc cttcctgtcg gcagcgaaca ccgcaccggt caggccatag
       61 ggggaggagc ggtcgcacag ttcgaggatt tccccgaagc ggtcatcctc gtaggtgtac
      121 agggtgagca cgggaccgaa gatctcctcc ctcatggtaa cgaattccgg atcggtggtc
      181 tcgatgaccg tgggctccac gaaatagcct gctgatttgt cgccccttcc tccgaagagg
      241 atgtcagctt cggtggacat gcgggctgcc gaaatgtatt tcatgcagtt gtcgaaggag
      301 gcttcgtcga tgacggcgtt gatgaagttt ctgaaatcct ccggcgatcc cgtcttcatg
      361 tcggcgatca tccgccccgc cagagacagg attttttccc tgaggctctt cggtatatag
      421 catctcgaaa cggcggagca cttctgcccc tggtattcga aggctccccg aaccatggcg
      481 gtaacggcgg cgtccacgtc cgccgtagga tggacgacga gaaaattctt ccctcccgtc
      541 tcccctacga gccgcgggaa ggaccggtag ttccgcaggt tttccgcgca ctgcatccac
      601 agcttcgaga acacgtccgt ggacccggtg aagtgtattc ccgccaggtc cgggtgtttt
      661 gtcactactc tgctgatcat ctcccccgaa ccgggaagga agttcaccac cccggcggga
      721 aggccggcct tctcgtagat tttcatcagg taatagctgg agagcaggga cgtgcccgcg
      781 ggtttccaga gcaccgtgtt ccccatcagc acgggggcga gaacaaggtt ggacgcaatg
      841 gccgtgaagt tgaagggggt caccgcaaaa acgaagcctt cgagagggcg gtactccatc
      901 ctgttgaggc tgtccctgtc caggcggggc tgttcggcgt atatggagga agcgtaggcc
      961 acgttgaacc gtatgaaatc cgccgcctcg gccacggtgt cgatctgggc ctgaaagacg
     1021 tttttgctct ggccgagcat ggtggaggcg agaaggaggt ccctgtactc cgtggtgaag
     1081 agttccgcga ttttgagcat cacggcggcc cgttcggtcc acggggtatt ttcccattct
     1141 ttcttcgccc tggccgcttc cttgacggct ctttccacgt ccgccggcga tgccttgcag
     1201 tgcgtcgcaa ggacgtggcc gtgatcatgg ggcatcacca cccgcccctt ttcttcgctt
     1261 ccgtactctc ttccccctat gatcaggggt atctccggaa cctcggaacg tacctttgcc
     1321 agggcggcct tcaacgaccg tttttccggc gtccccggtg caaagccgag gagaggctca
     1381 ttctccggtt cccggaacat gaaaaaactg ttgttcaata taatcccctc ctttcttcag
     1441 attcatctgc ttacaatttc ccggcatcag aaaataactg cagcggtttt gtttagatct
     1501 caatcttcag ggaagtgcaa cagcatgtca actttttgtc tcatttggcc tgttaaggtt
     1561 cattttgaca aaaccttccc tcccggaccg ttgtattgca aaaacacatc aatcaaggat
     1621 acaatatcag gagtgtacag cggcaacaat gcggcgaaag ggaacgtctc ccgttccggc
     1681 cggcggcgaa ggcgaaaagg gctgacggca gcggacccgt tcccggcaca cccccttcac
     1741 aggaggctgc tccatgggaa ttctctcagc ggacaagggc gcttactcca ggaaggtcga
     1801 cctcttcttc cggcatcccg tcgtggaggg ggcgctgctc ttcctcattt cggcatcggt
     1861 tctcgccgcc atagcgtcta tcctgttcga ccaccttttc cttgagtggc tgaatctgtt
     1921 cttttccgcc cttttctccg tggagctcac ggcccggtat tttacctacg gcgggaaaga
     1981 aaaggactat gtccgggact ggtggatgga ctgggcggcc accatcccct gggacgtctt
     2041 cctttccttt ctctttcccg gaggcggcgc ctccatgctc cggctgctcc gcctgccgag
     2101 gatcttccgg ctgctccggt tccggcggct gaagaagtcg gaagcggcgc gctggctgtc
     2161 ctaccgcttc cgccgcctga tggaagtttc catctggcgc cagtccctga cgctgctcct
     2221 cgtctccctc gtcttcgtgt gggtcttcac cgttcttctg gacagggtgg gaatgaactc
     2281 cgaacacggc ggcaacttct ggttttccct gatcaccatg atcagctccg acagcatttt
     2341 cgagatgcag gggcaggact ccctggtaaa ggggctggtg ctgctccttt catttatcgg
     2401 gatcgtcctg ttcaacggca tcctcatcgc catcatcatc ggcaagctca tggaacggct
     2461 gggcgagatg aaggacgccg gggaggtccg tgagcggggc catattctcc tcctcgggtg
     2521 gagcgagtgc attccccata tcct
//
LOCUS       DAOTHD010000242         2543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_48441_length_2543_cov_2.584405, whole genome shotgun sequence.
ACCESSION   DAOTHD010000242 DAOTHD010000000
VERSION     DAOTHD010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48441_length_2543_cov_2.584405"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="P5219_05105"
     CDS             complement(<1..827)
                     /locus_tag="P5219_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscN/YscN/HrcN family type III secretion system
                     ATPase"
                     /protein_id="HRX26166.1"
                     /translation="MTAAPEGDLLAILEQRLSGFQLTKINGRIVQVVGLVAESRGPDV
                     RVGDLCSIRYRHSTRAALSAEVVGFRGDRVLLMPLGNLKDIGPGCDVLSMERPLGVRV
                     GDSLLGRVLDGLGNPIDDRGPVAASGFYPLYAEPPHPLRRKMITSPLPVGVKVIDGLL
                     TLGTGQRIGIFAGSGVGKSTLLAMMARYTEADVNVIALVGERGREVREFLDRDLGEEG
                     RRRSVLVIATSDQPPLIRLKASLTATAIAEYFRDQGKNVLLMMDSVTRVARAQREVGL
                     "
     gene            complement(811..1704)
                     /locus_tag="P5219_05110"
     CDS             complement(811..1704)
                     /locus_tag="P5219_05110"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HRX26167.1"
                     /translation="MSKPLRPNVIRAVRLLPEAVRIGSSAGEERRAPESAPGEPAGAG
                     NHPGDLFTQLTEELSLLKAKLEESERENRDLASRAAVFEEELSRERVRLKEERGAMVS
                     ALESEAAAARKKAMSEGFEEGRKAGYEQGIDDARKEAAGEAEEKIRSAVDLLESIHRS
                     LEEHMDALAELRMPRLIRMWEFLLSRMLRREVSLDGETALRVLRGVLERISDKERVVV
                     YLSPEDADAVRGRKDEFGDLLRGVRHLEFVPDGNVDAGSCIVETNLGIYDARWRIQLE
                     QVSGEIDRLFIEGGTSDDGSS"
     gene            complement(1697..>2543)
                     /gene="fliG"
                     /locus_tag="P5219_05115"
     CDS             complement(1697..>2543)
                     /gene="fliG"
                     /locus_tag="P5219_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HRX26168.1"
                     /translation="LGVLKDAQEMLLAREYMARGGVDYARDILERALGPERAQNLLTR
                     ITASLQVRPFDFMRHTDAQQILGFIQGEHPQTVALIMSYLEPQQAASIIGGLPGAMQA
                     EVAKRIARMDRITPEVLREVERVLERKLSTVMGQDFTLAGGIDAIVDIINNADRATER
                     NIMEHLEENDPELAEEIKRRLFVFEDIIKMDDRSLQRVLREVEMKELSLALKGATEEL
                     RGKFFKNMSKRASEMLKEDMDYMGPVRVKDVEEAQQKVVNVVRALEDVGEIVISRGGE
                     EELVV"
BASE COUNT          429 a          850 c          757 g          507 t
ORIGIN      
        1 agccccacct cgcgctgggc cctggccacc ctggtcaccg agtccatcat gagaagaacg
       61 ttttttccct ggtcccggaa atactcggcg atggcggtgg cggtgaggga tgccttgaga
      121 cggatgaggg gaggctggtc ggacgtggcg atgacgagca ccgaacggcg gcgcccctcc
      181 tcgcccaggt cgcggtcgag aaattcccgg acttcccttc cccgttcccc caccagtgcg
      241 atgacgttca cgtcagcctc ggtgtacctg gccatcatgg cgagcagggt gctctttccc
      301 acacccgatc cggcgaatat gccgatcctc tgtcccgtcc cgagggtgag aaggccgtcg
      361 atgaccttga cgcccacggg aagaggggag gtgatcattt ttctgcgaag ggggtgggga
      421 ggttccgcgt agagagggta aaatccggaa gccgccacgg gcccccggtc gtcgataggg
      481 tttccaagtc cgtcgagaac gcggccgaga agggagtccc ccacacggac acccaggggc
      541 cgctccatgg agagaacgtc gcatcccgga ccgatgtcct tgaggttgcc caggggcatg
      601 agaagtaccc ggtcgccccg gaaacccacc acctccgcag acagggccgc cctggtactg
      661 tgtctgtacc ggatggaaca gaggtcgccc acccgcacgt ccgggcccct ggattcggcc
      721 acgaggccaa ccacctggac aatccggccg ttgatcttgg tgagctggaa tcccgaaagg
      781 cgctgctcga gaatggccag caaatccccc tcaggagctg ccgtcatcgg aggtccctcc
      841 ctcgatgaac agacggtcta tctccccgga cacctgttcg agctggatcc gccaccgggc
      901 gtcgtaaatt ccgaggttgg tctccacgat gcagctgccg gcatccacgt tgccgtcagg
      961 aacgaactca agatgacgaa cgccccgaag gagatcgccg aattcgtcct tccggccccg
     1021 aacggcgtcc gcatcctccg gactgaggta cacgacgacc cgttccttgt cgctgatccg
     1081 ctccagaact ccccggagca cccggagggc tgtttctccg tcgagggaca cttccctgcg
     1141 gagcatccgg gacagcagga attcccacat gcggatgagc ctgggcatcc ggagctccgc
     1201 cagggcatcc atgtgttctt cgagggaccg gtggattgat tccagcaggt caaccgccga
     1261 ccggatcttc tcctcggctt ccccggcggc ttctttccgg gcgtcgtcga tgccctgttc
     1321 gtatcccgcc ttccgtcctt cctcgaaccc ctcggacatc gcctttttcc tggctgcggc
     1381 ggcctcggac tcgagggcgg acaccatagc tccccgctct tctttcaggc gcacccgctc
     1441 cctggaaagt tcttcctcaa aaaccgccgc ccgggaggcg aggtcacggt tttccctttc
     1501 ggactcctcg agtttcgctt tcaggagaga gagttcctcc gtgagctggg tgaacaggtc
     1561 gccgggatga ttccccgccc cggcaggttc ccccggagca gactccggtg cccgccgctc
     1621 ttctcccgct gaactgccga tcctgacggc ctccgggaga agacgcaccg cccggatgac
     1681 attggggcga agaggcttag acaaccagct cttcctctcc tcccctggag atgacgattt
     1741 cgcccacatc ctccagggcc cggaccacgt tgaccacctt ctgctgggct tcttccacat
     1801 ccttcaccct gacggggccc atgtagtcca tatcctcctt gagcatttcg gaggcccgct
     1861 tggacatatt cttgaagaac tttccccgga gctcttcggt ggcgcccttg agggccaggg
     1921 agagctcctt catctccacc tcgcggagca ccctctggag ggaccggtcg tccatcttga
     1981 tgatgtcctc gaagacgaag agccgccgct tgatttcttc cgcgagttcg gggtcgttct
     2041 cttccaggtg ttccatgatg ttccgctccg tggcgcggtc ggcgttgttt attatgtcca
     2101 cgatggcgtc gatgccgcct gcaagggtaa agtcctgtcc catgacagtg ctcagcttcc
     2161 tttccagcac ccgctccacc tcacggagca cttccggagt gatccggtcc atcctggcga
     2221 tccgcttggc cacttcggcc tgcatggccc cgggaaggcc gccgatgatg gacgccgcct
     2281 gctggggctc gaggtaggac atgatgaggg ccacggtctg gggatgttct ccctggatga
     2341 agccgaggat ctgctgggcg tcggtgtgcc gcatgaagtc gaagggccgg acctgaaggc
     2401 tggcagtgat ccgtgtgaga aggttctgcg ccctttcagg gcccagggcc ctctcgagga
     2461 tatccctggc atagtccacg ccgcccctgg ccatgtactc cctggcgaga agcatttcct
     2521 gggcgtcttt cagcactccg agc
//
LOCUS       DAOTHD010000243         2540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_48499_length_2540_cov_3.058753, whole genome shotgun sequence.
ACCESSION   DAOTHD010000243 DAOTHD010000000
VERSION     DAOTHD010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48499_length_2540_cov_3.058753"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="P5219_05120"
     CDS             complement(<1..494)
                     /locus_tag="P5219_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26169.1"
                     /translation="MNAARPVPLVTASLVFLLLLSGIAAGSAPDPLTPADRSSPRDTL
                     TSFITHMNTGHHYLQQARMISRGAPGFFVHPPEAVEKGDLARYHFERAMQCLDLSEVP
                     YVLRDDVAREGALLLKEILDRIGLPPLGTIPGGKDWINTGDSRITRWNIPRTELQLYR
                     VESG"
     gene            765..1442
                     /locus_tag="P5219_05125"
     CDS             765..1442
                     /locus_tag="P5219_05125"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HRX26170.1"
                     /translation="MTPEFARGFRASVPAGIGVLVYGLVFGVLAAEKGVSAPQLAIMN
                     VAVFSGSAQLILVGMWSGGFPLWQVALAAAVVNVRYFLLTAAVAPLLEPYGKFGGAWR
                     VHFVTDENWAVTMAESRRRRVTGDFLVGGGVCVGLFWFFSVLAGHSLGFTIAAPERFG
                     LDFAFTALFTALAVTMWRGRRDIFPWAVSAAVAYAAFRLLPGAWYVLLGGAAGAAAAA
                     VRGGEEG"
     gene            1439..1750
                     /locus_tag="P5219_05130"
     CDS             1439..1750
                     /locus_tag="P5219_05130"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HRX26171.1"
                     /translation="MTLSGGNAWAVIVLAALFVYSIRFLGMAFGSRLPKNGPVKRAMD
                     ALPGTIFAALVFPGLAEAGLPGMAAAGVIFLAVRKTGSVFLAMILGMGTVALLRAVTG
                     N"
     gene            complement(1737..>2540)
                     /locus_tag="P5219_05135"
     CDS             complement(1737..>2540)
                     /locus_tag="P5219_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRX26172.1"
                     /translation="EERRGRFRALGIGSFGPVGLDPHSPSYGRITTTPKEGWRNADIL
                     GTFRRAFPGIPIGFDTDVNAAAMGEHRWGAGRSLGDFVYITMGTGIGAGVMAGGKLLH
                     GLVHPEAGHLRIPRLPGDAFPGICPFHGDCWEGLCSGPAILKRTGIPAEDLPGNHEAW
                     PLTARYTAIALADLVCALSPRRIILGGSVPGAGLLGRDRFLAMVRNGVREALGGYISV
                     PELSGDLSGYIIPPLLEENAGICGALALAETAWEEDRHPTDSRNVVSSR"
BASE COUNT          398 a          733 c          892 g          517 t
ORIGIN      
        1 tcgccggatt cgaccctgta cagctgaagt tccgtgcggg ggatgttcca tcgggtgatt
       61 ctggagtctc cggtattgat ccagtccttt ccgccgggaa tggtcccgag cggcggaagg
      121 ccgatgcggt caaggatttc cttgagaagc agcgcccctt cccgggcgac gtcatccctg
      181 aggacgtaag gtacttcaga gaggtccagg cactgcatgg cccgctcgaa gtggtacctg
      241 gccaaatctc ctttttccac cgcttcgggg gggtggacga agaagccggg agcgccccgg
      301 gagatcatcc tggcctgctg caggtagtgg tgccccgtgt tcatgtgggt gataaaggat
      361 gtgagggtgt cccggggaga cgaccggtcg gctggggtca ggggatccgg ggctgacccg
      421 gcggctattc cggacaggag caggaggaac accagggaag ctgtgacaag cggaacaggg
      481 cgggcggcat tcatggaata tcatctcctt gttttttcgt cgggcatccg ctccggcccg
      541 ttcccgtccg tgcccgggac tgtccgggag catcctgcgg ctgaaacagt atcagagggg
      601 gcttcccttt gcaaatgttt cggacgattt tttctccccc ggacctggag tatactcttt
      661 aatacttggt tcagctccag gtaaaggagg atggggaaga tgcgcttaat cccccggtat
      721 gacctgtttc cccgaatgtg cccggaagag ggaggagcgg caggatgacg cctgaattcg
      781 cgagaggctt ccgggcgtcg gtgcctgccg ggatcggcgt gctggtgtac gggcttgtct
      841 tcggggtgct ggccgctgag aagggagtat cggctcccca gctggcgata atgaatgtgg
      901 ctgttttttc aggctcggcc cagctcattc tcgtgggaat gtggagcggc ggtttccccc
      961 tctggcaggt ggccctggcg gctgccgtgg tgaatgtgcg gtatttcctc ctcaccgcgg
     1021 cggtggctcc tctgctcgaa ccctacggga agttcggggg ggcctggagg gtccacttcg
     1081 tcaccgacga gaactgggcg gtgaccatgg cggagagccg cagaagacgg gtcacgggtg
     1141 attttctggt gggcggcggg gtctgcgtgg ggctgttctg gttcttcagc gttctggcag
     1201 gccacagtct tgggtttacc attgcggcgc cggaacggtt cggcctcgat ttcgccttca
     1261 ccgccctttt cactgccctt gccgtcacca tgtggcgagg acggagagat atcttcccct
     1321 gggctgtttc cgcagcagtg gcgtatgctg ctttcaggct gcttccaggc gcatggtatg
     1381 tcctcctggg gggagcggca ggcgcagccg cggccgcggt gaggggaggg gaagaagggt
     1441 gaccctttcc ggcgggaatg cctgggcggt cattgtcctc gccgccctgt tcgtctactc
     1501 catacggttt ctgggcatgg ccttcggcag ccgcctcccg aagaacggac cggtgaaaag
     1561 ggccatggat gcccttccag ggaccatctt cgccgccctt gtctttccgg ggcttgcgga
     1621 agccggcctt cccggaatgg cggctgccgg ggtgattttt ctggcggtgc ggaaaacggg
     1681 gagtgtcttc cttgccatga tcctgggcat gggaacggtg gccctcctca gggcggtcac
     1741 cgggaactga cgacattccg ggagtccgtg ggatggcggt cttcctccca tgccgtttcg
     1801 gccagggcga gggcgccgca gatcccggcg ttttcctcca gcaggggcgg aatgatatat
     1861 cctgacaggt cccctgacag ttcggggacg gaaatgtagc cgccgagggc ttcccgaacc
     1921 ccgtttctca ccatggccag aaaccggtcc cgccccagca gtccggcccc ggggacactg
     1981 cccccgagga tgattctccg gggtgagagc gcgcagacca ggtcggcgag ggcgatggcc
     2041 gtgtaccggg cagtgagggg ccacgcctcg tggtttccgg gaaggtcttc agcagggatt
     2101 cccgttcttt tcaggatggc aggcccggag cacaggcctt cccagcagtc gccgtggaag
     2161 gggcagatcc ccgggaaggc gtcgccggga agacggggga tgcggagatg ccctgcttcc
     2221 gggtggacaa ggccgtgcag gagtttccct cctgccatga ccccggcacc gatgccggtg
     2281 cccatggtga tgtagacgaa gtcccccagg ctgcggccgg cgccccatcg gtgttcaccc
     2341 atggcggcgg cgttcacgtc cgtgtcgaag cctattggaa tgccggggaa agctcgcctg
     2401 aaggtgccca ggatgtccgc attccgccag ccctccttgg gcgtggtggt gatgcggcca
     2461 taggatggag agtgaggatc gaggcctacc gggccgaagg atccgatgcc cagggcgcgg
     2521 aaacggcccc ggcgctcttc
//
LOCUS       DAOTHD010000244         2530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_48776_length_2530_cov_3.186263, whole genome shotgun sequence.
ACCESSION   DAOTHD010000244 DAOTHD010000000
VERSION     DAOTHD010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48776_length_2530_cov_3.186263"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /gene="dctP"
                     /locus_tag="P5219_05140"
     CDS             complement(<1..367)
                     /gene="dctP"
                     /locus_tag="P5219_05140"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRX26173.1"
                     /translation="MFGKKTFSLALLFVFLAASFAYGAPVVLKLSNVLPDGNPTTESM
                     VFFAEKVKEKTGGSVEVRLFNNSALGGQREALESMQAGTLEMCMANAGPMAQFVPAMD
                     VLSLPYVFQSTEHMFKALNG"
     gene            complement(524..1807)
                     /locus_tag="P5219_05145"
     CDS             complement(524..1807)
                     /locus_tag="P5219_05145"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRX26174.1"
                     /translation="MAATIFAVFILLLVIRMPVSFSLAISAAIALVFAGSMDPLIVVQ
                     RMYTSVESFSLIAIPFFILSGGFMESGGISRRLISFSSSLVGHIRGGLSMISIVAAMF
                     FAGISGSTAADTAAIGSVLIPAMEKKGYGKDLATSVVGTAGAIGIIIPPSIPMIILGI
                     TGSISIGGLFLGGVFPGLLMGLALMVTSGIFARKRNLPAERRATWKERWHAFTDSILA
                     LMTMVIIMGGILSGVFTATEASVVAAFYSFLVGRFVYGELKWKDIPGIMLKTGITTGV
                     VVLCIATASSFGWILAAEQIPDKIAKAVFALSDNRLVILFLMNALMLFMGTFLDISPI
                     IIILVPILFPIARQLGVTPIHFGIMTIVNMAIGQCTPPVGISLFVATGIAKAKLGEIL
                     GAYSKYLLTMILVLALITVFPQIITFLPQMIMGLK"
     gene            complement(1809..2291)
                     /locus_tag="P5219_05150"
     CDS             complement(1809..2291)
                     /locus_tag="P5219_05150"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRX26175.1"
                     /translation="MERLDAFAARLERLMLIVLLAVMVILGLLQIISRFVIKAPIMWS
                     EALLMYMFVWFSFLGAGLAVRELSHFEVEIFVVRLPQTIQKLLSLAVYLMIFAFAVFM
                     IWKGLFLVKLNQRQLMAMMPFTMSGPYMILPLSGTFMAIHSLAHLTGLFSRHHTEWRD
                     "
     gene            complement(2314..>2530)
                     /locus_tag="P5219_05155"
     CDS             complement(2314..>2530)
                     /locus_tag="P5219_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HRX26176.1"
                     /translation="FNLPDDGVIFHFMGQPSELRHIVVRSGEAVISPSWSIHSGVGST
                     SYSFVWGMAGENQTFSDMDGVPMSLLR"
BASE COUNT          573 a          697 c          795 g          465 t
ORIGIN      
        1 tgccgttgag ggccttgaac atgtgttccg tgctctggaa gacataggga agggacagca
       61 cgtccatggc ggggacgaac tgggccatgg gccccgcgtt ggccatgcac atctcgaggg
      121 ttccggcctg catgctttcc agggcctccc gctggccgcc aagggcgctg ttgttgaaca
      181 ggcggacttc aaccgatccg ccggtcttct ccttcacctt ttcggcgaag aagaccatgg
      241 attcggtggt gggattgccg tcggggagaa cgttggagag cttcagcaca accggtgccc
      301 cataggcaaa ggacgccgca agaaaaacga aaagaagcgc gagactaaag gtctttttac
      361 cgaacatgta attacctcct tcttttctgc ttccacatcc gctgctccct cctgcggaga
      421 gggagtctcc caagcctgga ctgctgcctt cctcttagag catgggtgtt tttccttcca
      481 ctcccccctt cccgggattt gggtcctttc cattcgcaga agtctacttc agccccataa
      541 tcatctgggg cagaaaggtg atgatctgcg gaaacactgt gatcagcgca agaaccaata
      601 tcatggtaag gagatatttg gagtaggctc ccaggatctc tccgagtttt gccttcgcga
      661 tccccgtggc gacgaaaagg gaaatgccca ccggcggggt acactgcccg atggccatgt
      721 tcacgatggt catgatgccg aagtggatgg gggtcacgcc gagctgtctc gcgatgggga
      781 agagaatggg gacgagaatg atgatgatgg gcgaaatgtc gaggaacgtg cccatgaaca
      841 gcatgagggc gttcatgagg aagaggatga ccagccggtt gtccgacagg gcgaaaacag
      901 ccttcgctat cttgtcgggg atctgctccg cagcgaggat ccagccgaag ctggaggcgg
      961 tggcgatgca gagaaccacc acgcctgtgg tgatgcccgt cttgagcata atccccggta
     1021 tgtccttcca cttcagctcc ccgtagacga accggccgac gagaaaggaa tagaaggccg
     1081 ccactaccga ggcctccgtg gcggtgaaga cgccggagag aatgcccccc atgatgatga
     1141 ccatggtcat gagggccagg atggaatcgg tgaaggcatg ccaccgttcc ttccacgttg
     1201 cgcgccgttc tgcgggaaga ttccgcttcc gggcgaagat gccgctggtg accatgaggg
     1261 ccaggcccat gagcaagccg gggaaaaccc cgccgaggaa aagacctcct atggatatgc
     1321 ttcccgtgat gcccaggatg atcatgggga tgctcggagg gatgatgatc cctatggcgc
     1381 ccgccgttcc caccacgctg gtggccaggt ccttcccgta gcctttcttc tccatggccg
     1441 ggatgaggac gctgccgatg gccgccgtat ccgccgccgt ggagccggaa atgccggcga
     1501 agaacatggc cgccacgatg ctgatcatgg acaggccgcc ccggatatgg cccaccaggc
     1561 tggacgagaa ggaaatgagc cgcctggaga tgccgccgct ctccatgaag cccccggaga
     1621 gaatgaagaa ggggatggcg atgagggaga aggactcgac ggaagtgtac atccgctgca
     1681 ccacgatcag gggatccatg cttccggcga agaccagggc aattgccgcg gagatggcca
     1741 gggagaagga gacgggcatc cggataacga gcaggagaat gaagaccgca aagattgtgg
     1801 ctgccatgtc agtccctcca ctccgtatga tgccgggaaa acagcccggt gagatgtgcc
     1861 agggaatgga tcgccatgaa ggtgccgctc aggggcagga tcatgtaggg gccgctcatg
     1921 gtgaagggca tcatggccat gagctgccgc tggttcagct tcacgagaaa gagccctttc
     1981 cagatcatga acacagcgaa ggcgaagatc attaaataga ccgcaaggga aaggagtttc
     2041 tggattgtct ggggaagccg gaccacgaag atctccacct cgaaatggga aagttccctt
     2101 accgccaggc cggcgccgag aaagctgaac cagacgaaca tatacatcag gagggcctcc
     2161 gaccacatga tgggggcctt gatgacgaaa cggctgatga tctggaggag ccccaggatt
     2221 accatgaccg caaggagaac gataagcatc aatcgttcca ggcgcgccgc gaaggcatcc
     2281 agtctttcca tggagggccg gctctcccct ctgctagcgg agcagggaca tgggaacgcc
     2341 gtccatgtcg gaaaaggtct ggttttctcc cgccatgccc cagacgaagc tgtagctggt
     2401 gcttccgacc cctgaatgga tggaccagct cggggagatg accgcttcgc cgctgcggac
     2461 cactatgtgc cgcagttcgg agggttgtcc cataaagtgg aagatcaccc cgtcgtcggg
     2521 gaggttgaag
//
LOCUS       DAOTHD010000245         2529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_48805_length_2529_cov_2.981811, whole genome shotgun sequence.
ACCESSION   DAOTHD010000245 DAOTHD010000000
VERSION     DAOTHD010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48805_length_2529_cov_2.981811"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="P5219_05160"
     CDS             <1..433
                     /locus_tag="P5219_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRX26177.1"
                     /translation="AFMGKSTLVGDLGAGQTTKLVNQSIVAVNIAAVAEGMAFAKKAG
                     VDPEKVFNAIRGGLAGSQCMEDKAPRMFEGRYNPGFRINLHIKDLNNVLETSRSLHVS
                     MPLTAQLMEMFQNLAADGHETLDHGALGLFYEKLNTLSLKK"
     gene            588..1643
                     /locus_tag="P5219_05165"
     CDS             588..1643
                     /locus_tag="P5219_05165"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRX26178.1"
                     /translation="MKKFATIIAVVLIAALALGGTAFAADKIWPKGTPTVIVGFGAGG
                     GTDTSVRPMVALMSKYLNETINVANMPGAASAVAAEHVLGLPGDGYTMFATGSGCFGG
                     FKIKETSNSGWEEWTSWHPFQGPAAILVGANSEMKTFDDVAKALKEGKINMGISGFGN
                     GPHVIMQAIAEVAGINEINYVTFDGDRNVATAVLAGEVEVGIVTFSSAIDMAQAGTIR
                     AVTVMMKEPMKITDTITIDPIVKVLPGSDKIPDLSETWPICIRRDAPQEIKDKITEAF
                     HWALKQPEILEYTKQKGLNIVGLTGEEADRFLSLQISGYSWVLYNAKMAEKSPADFNI
                     PKLADWNWETEKKKLNK"
     gene            1645..2133
                     /locus_tag="P5219_05170"
     CDS             1645..2133
                     /locus_tag="P5219_05170"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRX26179.1"
                     /translation="MTGRVHKGSGADYISAVCLIVFGAAFSAAALKMRVFNNSLLVSP
                     GLFPLILGGVFILLGFLLLRSAAKRGGKDQALHVLGKENLTAFFSSPKVRKGTVLLLL
                     VVAYVAAVAYIPFLWATAGYLIVTFLYLKAMKLHWSILLAFAAAWVITAAFRDLFRIP
                     MP"
     gene            2149..>2529
                     /locus_tag="P5219_05175"
     CDS             2149..>2529
                     /locus_tag="P5219_05175"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRX26180.1"
                     /translation="MEILHLLADEAAQVGVSLVGALNPTILGLIFGGSLLGMLVGAIP
                     GLTATMALALLINLSYGMPLENAVAFLLAVYVGAVSGGLNAAIMINIPGTPSAAATCL
                     DGFPLAKQGKGGLAIGTGLIASFVG"
BASE COUNT          529 a          837 c          717 g          446 t
ORIGIN      
        1 cgccttcatg ggcaagagca ccctcgtggg cgatctcggc gcagggcaga ccaccaagct
       61 ggtgaaccag agcatcgtgg ccgtgaacat cgccgccgtc gccgaaggca tggccttcgc
      121 gaaaaaggcc ggagtagacc ccgaaaaggt gttcaacgcc atccggggcg gcctcgccgg
      181 cagccagtgc atggaggaca aggccccccg catgttcgag ggcagataca acccgggctt
      241 ccggatcaac ctccacatca aggacctgaa caacgtcctc gagacgagcc gctcgctcca
      301 cgtgtccatg cctctgacgg cccagctcat ggaaatgttc cagaacctgg ccgccgacgg
      361 ccacgagacc ctggaccacg gcgccctggg cctgttctac gaaaagctca acaccctgtc
      421 gctgaagaaa tagggaaagc agtccggaag ccgctccctc cggggagcgg cacacctgcg
      481 gaaaaggagg gatgcctggg gggaaagccg ggtgatccag aaagggtagg cggaaagaca
      541 gcttctgttc cacagatacc aatacctgac aagaaggagt ggttattgtg aagaagttcg
      601 ctaccattat cgccgttgtc ctcattgcgg ccctcgccct cggcggcacc gccttcgcgg
      661 cggacaagat ctggcccaag ggaaccccca cggtcatcgt cggcttcggt gccggcggcg
      721 gcaccgacac gtccgtgcgg cccatggtcg ccctcatgtc caagtacctg aacgaaacca
      781 tcaacgtggc caacatgccc ggcgcagcca gcgcggtcgc ggccgagcac gtcctcgggc
      841 tcccgggaga cggctacacc atgttcgcca ccggatccgg atgcttcggc ggcttcaaga
      901 tcaaggaaac gtccaattcc ggctgggaag agtggacgtc ctggcatccc ttccagggtc
      961 ccgcagccat cctcgtgggc gccaacagcg aaatgaagac ctttgacgac gtggcgaagg
     1021 ccctcaagga aggcaagatc aacatgggca tctccggctt cggcaacgga ccccacgtca
     1081 tcatgcaggc catcgcggaa gtcgcgggca tcaacgagat caactacgtc accttcgacg
     1141 gcgaccggaa cgtggccacc gcagtcctcg ccggtgaagt cgaagtgggc atcgtgacct
     1201 tctcctccgc catcgacatg gcccaggcag gaaccatccg cgccgtcacc gtcatgatga
     1261 aggaacccat gaaaatcacc gacaccatca ccatcgatcc catcgtcaaa gttcttcccg
     1321 gcagtgacaa gatccccgac ctgagcgaaa cctggccgat ctgcatccgc cgcgacgctc
     1381 cccaggagat caaggacaag atcaccgagg ccttccactg ggccctcaag cagcctgaaa
     1441 ttcttgaata cacgaagcag aagggcctga acatcgtcgg gctcaccggt gaggaagccg
     1501 accgcttcct ttccctccag atctccggtt actcatgggt gctctacaac gcgaagatgg
     1561 cggagaaatc ccccgccgac ttcaacattc ccaagctcgc cgactggaac tgggagaccg
     1621 aaaagaagaa gctgaacaaa taacatgaca ggaagagtgc acaagggctc cggggcggac
     1681 tacatctccg ccgtatgcct gatcgtcttc ggggcggcgt tttcagccgc cgccctgaag
     1741 atgcgggtat tcaacaactc ccttctcgtc tcccccgggc tttttcctct cattctggga
     1801 ggcgtgttca tccttctggg cttcctcctt ctccgctccg cagcaaagcg gggcggaaag
     1861 gaccaggccc tccatgtcct cgggaaggaa aacctgaccg cttttttctc ctctccgaag
     1921 gtacggaaag gaaccgtcct gctcctcctc gtcgtcgcgt atgtcgcggc ggtggcatat
     1981 atcccgttcc tctgggcaac agcgggatat ctcatcgtca cgttcctcta cctcaaggcc
     2041 atgaagctgc actggagcat tctcctggcc tttgcggcgg cctgggtgat caccgcagcc
     2101 ttccgggatc tcttccggat ccccatgccg taagaaaggg aggagatcat ggaaatactg
     2161 catcttctcg cggatgaagc agcacaggtc ggggtctccc ttgtgggagc cctcaacccg
     2221 acaattctcg gactgatctt cggtggaagc ctcctgggaa tgctcgtggg agcgatcccc
     2281 ggactcacgg ccaccatggc cctggccctg ctgatcaacc tctcctatgg catgcccctg
     2341 gagaacgccg tggcctttct tctcgccgtg tacgtagggg ccgtctccgg aggtctcaac
     2401 gcggccatca tgatcaacat ccccggcacc ccatcggcgg cggccacctg cctcgacggc
     2461 ttccccctgg cgaagcaggg caagggcggc cttgccatcg gcacggggct catcgcctcc
     2521 ttcgtgggt
//
LOCUS       DAOTHD010000246         2528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_48830_length_2528_cov_3.782450, whole genome shotgun sequence.
ACCESSION   DAOTHD010000246 DAOTHD010000000
VERSION     DAOTHD010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48830_length_2528_cov_3.782450"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="P5219_05180"
     CDS             <1..1180
                     /locus_tag="P5219_05180"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRX26181.1"
                     /translation="LALLSVLLSASAGALESFELYRNLRSLNRDLADRMSQLEESRRE
                     LEEYRKFLEQQVERRTMELSRANEEMKMEIAERMRTEEDLRSSEEMLRLAVEGMGDGV
                     WIWNGTTGKTDLSPLSAEILCGDDEDPASCILESTSGEAWGRIIHEDDRERLSRKKAR
                     CLSGEVPAYSAEYRVVGKDGKERWILERGRVVRKDREGRPLAIAGTHSDITGRKKLED
                     HIRFQATHDFLTGLPNRFLFEDRLTQVLARAKRQKGKAALFFVDMDDFKKVNDRYGHL
                     AGDTLLKEAGSRIVACVREMDTVCRLGGDEFTVILPAVAGPGEAESIAGRIMDAFREP
                     FLLGGDTIFLSLSVGIGIYPDHGNTVEQLLANADEAMYRAKGQGKRICFLSPRNTSSS
                     "
     gene            1346..>2528
                     /gene="larA"
                     /locus_tag="P5219_05185"
     CDS             1346..>2528
                     /gene="larA"
                     /locus_tag="P5219_05185"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HRX26182.1"
                     /translation="MRVSIPYGSSRISCDITDSRLRAVLAPTLPNASAKNPGDELVKA
                     SLARPVGSLPLRELARGKKRVVILSSDHTRPVPSAVIMPLLLEEIRGGNPGADITILV
                     ATGCHRCTTDDELEKRYGTEIVRTVKIVVHDSRDESGHVAAGTLPSGGELVLNRLAVE
                     ADLLVAEGFIEPHFFAGFSGGRKSVLPGVAAYRTVLANHCAEFIASPGARTGILEGNP
                     IHRDMVYAARKAGLAFICNVVLDPEKRIVAAFSGDMEKAHEEGCAFLARFAKVQAAPA
                     DIVITGNGGYPLDQNIYQAVKSMTAAEASCRPGGVIIEAAECRDGHGGEAFFHAFRDI
                     PTPRGVQESILTRGRDSTEPDQWEIQIFARILTNHPVILVSSAPRETAEYMNMKWASS
                     LQ"
BASE COUNT          496 a          779 c          806 g          447 t
ORIGIN      
        1 cctcgccctt ctctcggtgc tgctctccgc ctccgccggt gccctggaga gtttcgagct
       61 gtaccggaat ctccggagcc tgaacaggga tctcgctgac cggatgtccc agctcgagga
      121 atcccggaga gagctcgaag agtaccggaa attccttgag cagcaggtcg agcggagaac
      181 catggagctc tcccgggcca acgaagagat gaagatggaa atcgccgaga ggatgcggac
      241 ggaagaggat ctccgttcaa gcgaggagat gctccggctc gccgtggagg gcatggggga
      301 cggagtgtgg atctggaacg ggacgacggg caagacggac ctttcccctc tttcggcgga
      361 gattctgtgc ggggatgatg aagatcctgc atcctgcatc ctggaatcca cctccgggga
      421 ggcctggggg cggatcatcc acgaggacga ccgggaacgg ctttcacgga agaaagcgcg
      481 gtgtctctct ggagaagtgc cggcgtacag cgcggaatac cgggttgtgg gaaaggacgg
      541 aaaggagcgg tggatcctcg agcggggaag agtcgtccgg aaggaccggg agggacgccc
      601 tctcgccatt gcgggcaccc actcggacat caccggaaga aaaaaattgg aggatcatat
      661 ccgcttccag gcgacccacg atttccttac gggccttccg aaccgctttc tcttcgagga
      721 ccggctgacc caggtgctgg cccgggcaaa gcggcagaaa gggaaagccg cccttttctt
      781 cgtggacatg gatgatttca agaaggtgaa cgaccggtac ggtcacctgg cgggcgatac
      841 tcttctcaag gaagcgggaa gccgcatcgt ggcctgcgtg cgggaaatgg acaccgtctg
      901 caggcttggg ggagacgagt tcaccgtcat cctgccggcc gtcgccggtc ccggcgaggc
      961 ggaatcaatt gccggccgca tcatggacgc cttcagggag ccctttctcc tgggagggga
     1021 tacgattttt ctctccctgt cggtggggat cgggatctac cctgaccacg gcaacaccgt
     1081 ggagcagctc ctggccaacg ccgacgaagc aatgtacagg gccaaggggc agggaaagcg
     1141 catctgcttc ctgtctcccc ggaacacctc ctcctcctga cggaggctct tttttgcgaa
     1201 gggttcaccg ttgacctttt cccctttttc cgcaacaata cgtccggaat atccggcatt
     1261 gccgcttccg gagattctct tcccgcggcc ctgtccggcc cctgcggggc attgacggtt
     1321 cccgcggaaa aggaggcgat tccccgtgcg ggtttccatt ccctacggca gttcacggat
     1381 tagctgcgac atcactgatt ccaggcttcg ggccgttctt gccccgacgc tcccgaacgc
     1441 ttcggcgaag aatccggggg acgagctcgt caaagcgtcc ctcgcccgtc ccgtcggctc
     1501 ccttcccctc agggagctcg cccggggaaa gaagcgcgtg gtgatcctgt caagcgacca
     1561 cacccggccc gtgcccagcg ccgtcatcat gccccttctc ctggaggaga tccgcggcgg
     1621 gaatcccggg gcggacatta ccattctcgt cgccacggga tgccacaggt gcaccactga
     1681 cgatgaactc gagaagcggt acggcactga gattgtccgc accgtgaaga tcgtggtcca
     1741 cgacagccgg gacgagagcg gccacgtggc cgcggggaca cttccctccg gcggggagct
     1801 tgtcctgaac cggcttgccg tggaagcgga ccttctcgtg gcggaaggat tcatcgaacc
     1861 ccacttcttc gccgggtttt ccggcgggcg gaagagtgtc cttcccggcg tggcggccta
     1921 caggacggtg ctggccaacc actgcgccga gttcatcgcc tcccccggcg caagaacggg
     1981 gatcctcgaa ggcaatccca tccaccggga tatggtctac gccgcccgga aagccgggct
     2041 cgccttcatc tgcaacgtgg tcctcgaccc ggaaaagcga atcgtcgccg cattcagcgg
     2101 agatatggaa aaagcccacg aagaagggtg cgcctttctg gcccgcttcg cgaaggtgca
     2161 ggccgcaccg gcggatatcg tgatcacggg caacggcgga tatcccctcg accagaacat
     2221 ctaccaggca gtgaagagca tgaccgccgc cgaggcctcc tgcaggccgg gaggggtcat
     2281 catagaagct gcggaatgca gggacggtca cggaggcgag gctttcttcc atgccttccg
     2341 ggatatcccc acccccaggg gcgtgcagga gagcatcctc accaggggga gggacagcac
     2401 cgaaccggac cagtgggaaa tccagatctt cgccaggatc ctcacgaacc atcccgtcat
     2461 tctggtctcg tcggctccga gggagacggc ggagtacatg aacatgaaat gggcttcctc
     2521 tcttcagg
//
LOCUS       DAOTHD010000247         2524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_48935_length_2524_cov_3.205346, whole genome shotgun sequence.
ACCESSION   DAOTHD010000247 DAOTHD010000000
VERSION     DAOTHD010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48935_length_2524_cov_3.205346"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..745
                     /locus_tag="P5219_05190"
     CDS             101..745
                     /locus_tag="P5219_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRX26183.1"
                     /translation="MNYKRFPLGPLWTNTYLVWDDSGRGFLADPAGDPGQIDEFARAR
                     GISVEKILLTHGHLDHAGGLPAVLERFGAPVYVPEKDASLVASPDPGALEWMGYDFKG
                     TDDFSTLRDGDVLEVGCMKVTVLATPGHTPGSSCYLAEEESGSLLLSGDTLFARSVGR
                     TDLPGGDPDMLDRSILKLAALPDGLQVLPGHGPETTIGAERKHNPFWPDGATTE"
     gene            742..1413
                     /gene="radC"
                     /locus_tag="P5219_05195"
     CDS             742..1413
                     /gene="radC"
                     /locus_tag="P5219_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRX26184.1"
                     /translation="MKLAALPSEEWPRERLRRRGASSLSLAELLAVLLRTGSKGRDVL
                     ELASELLREFGNAKGLSRATEEEMLSFPGLGEAKACVLLAALELGKRIHSEKCEPEDG
                     LPWEKRLSSIAAELAAEEREYIVGLHVRRNGTLITSDIISYGGPDGAFLDVKYLLRRA
                     VRLDSWGLVLVHNHPDGTLNPSREDRMLTDYLAGRTKMLDIRFIGHYVVANGKHVSIP
                     LPGDA"
     gene            1454..2488
                     /locus_tag="P5219_05200"
     CDS             1454..2488
                     /locus_tag="P5219_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRX26185.1"
                     /translation="MFGADIGIDLGTANIVVFVKGRGIVLSEPSAVAVRKRPKGGAAD
                     VIAVGKEAKAMAGKTPTGVSTIWPLQDGVIANFDMTEELIRHCLRKAGGGRGLLANPR
                     VVISVPAEVTEVERKAVVDATLGAGAREAYVVEEPVSAALGVGLPIQEPRGSMILDIG
                     GGTSEVAVLSLGGIVVTNSLRAAGKDMDNAIVAMLRQRYALLIGETTAEEVKIGIGSA
                     LPLDKELEMAVKGRDLADGLPKADMVTSVEVREALEPIVLRIEDMVKVALEQTPPELA
                     KDIVDQGILLSGGVSQLRGLAERLSRALNTPVVVAEDPLFAVARGVGKILDNLDDMRK
                     VLLSVERGSK"
BASE COUNT          507 a          735 c          841 g          441 t
ORIGIN      
        1 tagccaacga cggccccgtg acgctcatac tcgaaaaaga atccggaagc ggagattccc
       61 gcaacggctg aaaaatacgg acaacggagg atgatcggac atgaactaca agcgtttccc
      121 tctcgggcct ctctggacga acacctacct ggtctgggac gattccggca ggggattcct
      181 ggccgacccc gccggagatc ccggccagat tgacgaattt gcccgggccc ggggaatctc
      241 cgtagaaaaa atactgctta cccacggtca tcttgaccac gcgggggggc ttccagccgt
      301 tctggaacgg ttcggtgccc ccgtgtacgt cccggaaaag gacgcctccc tggtggcatc
      361 ccccgatccg ggcgccctgg agtggatggg gtacgacttc aagggcacgg acgacttttc
      421 gaccctccgg gacggagacg tgctcgaggt gggatgcatg aaggtcactg ttctggccac
      481 tcccgggcac accccgggaa gttcgtgcta cctggcagaa gaggagagcg gcagtctcct
      541 gctctccggt gacacccttt ttgcccggag tgtcggcagg acggatcttc ccgggggcga
      601 tccggacatg ctcgaccggt ccattctcaa acttgcggcg cttcccgacg gactgcaggt
      661 gctgcccggg cacggaccgg agaccaccat aggcgccgag aggaaacaca atcccttctg
      721 gccggacgga gcgacgacgg aatgaagctt gccgcccttc ccagtgaaga atggccgaga
      781 gagcgcctgc ggcgcagggg ggcgtcctcg ctctccctgg cggagctgct ggcggtcctt
      841 ctccgcaccg gcagcaaagg cagggatgta ctggaacttg cgtcggaact cctcagggaa
      901 ttcgggaacg caaaggggct ttcccgggcg acggaagaag aaatgctctc ctttcccgga
      961 ctgggtgaag cgaaggcctg cgttctcctg gcggccctgg agctggggaa gcggattcat
     1021 tccgaaaagt gcgagcccga ggacgggctc ccgtgggaga agcgcctttc ttccattgcg
     1081 gcggagctcg ccgccgagga gcgggaatat atcgtgggac tccacgtgag acggaacggg
     1141 accctcatca cctccgacat aatttcctac gggggaccgg acggagcttt tcttgatgta
     1201 aaatacctct tgcggagggc agtccgtttg gactcctggg ggctcgtcct tgtgcacaat
     1261 caccccgacg gaaccctcaa ccccagcagg gaagacagga tgctgactga ctaccttgcc
     1321 ggaaggacaa agatgctcga tatcaggttc ataggccatt atgtggtggc gaacgggaag
     1381 catgtttcca taccgcttcc cggagatgcc tagaaagtgg ggactgaagc aggtgttcgg
     1441 attattcaag gggatgttcg gcgctgatat tggcatcgat ctcggaacgg ccaatatcgt
     1501 ggtgtttgtg aagggcaggg gtatcgtgct cagcgagcct tccgccgtgg cagtgcggaa
     1561 acgcccgaag ggcggcgccg ccgacgtgat agccgtcggg aaggaagcaa aggccatggc
     1621 gggaaaaacc cccaccgggg tttccaccat ctggcccctt caggacgggg tgatcgcgaa
     1681 ctttgacatg accgaggagc tcatccgcca ctgcctccgg aaggcggggg gcggccgggg
     1741 actgctggcc aacccgcggg tggtcatctc cgttcccgca gaagtgaccg aggtggaacg
     1801 gaaagccgtt gtggatgcca ccctcggcgc cggggccagg gaagcctacg tggtggagga
     1861 gcccgtctcg gcggccctcg gcgtggggct tcccatacag gaacccaggg gcagcatgat
     1921 cctcgacatc gggggcggca cgagcgaagt ggcggtgctc tccctcgggg ggatcgtggt
     1981 tacaaactcc ctgagggccg ccggcaagga tatggacaac gccatcgtgg ccatgctccg
     2041 gcagaggtac gccctgctca tcggggagac cacggcggaa gaggtcaaga tcggcatcgg
     2101 atcggccctt cccctcgaca aggagctgga gatggcggtg aaggggcggg atctcgctga
     2161 cggtctcccg aaggccgaca tggtgacctc ggtggaagtc agggaagctc tcgagcccat
     2221 cgtcctgcgc atcgaggaca tggtcaaggt cgctctcgag cagacgcctc ccgagcttgc
     2281 gaaggacatc gtggaccagg gcatcctgct gtccggaggg gtctcccagc tccgtggact
     2341 ggcggaaagg ctctcaaggg ccctgaacac tcccgtggtc gttgctgaag atcctctctt
     2401 cgccgtggcc cggggagtgg gcaaaattct cgacaatctc gacgacatga gaaaggtcct
     2461 cctttccgtg gaaaggggat ccaagtagaa gctcaccgga gtggggaaag gtttgcatga
     2521 cgga
//
LOCUS       DAOTHD010000248         2522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_48988_length_2522_cov_3.605594, whole genome shotgun sequence.
ACCESSION   DAOTHD010000248 DAOTHD010000000
VERSION     DAOTHD010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48988_length_2522_cov_3.605594"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="P5219_05205"
     CDS             <1..606
                     /locus_tag="P5219_05205"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HRX26186.1"
                     /translation="EPKRVKFRAVLAEGVPKKNVPVNARIIGKPVDDFRLKAVVVEPA
                     VVTVEGPLAKLDRVTKLDTETIDITDITKEQNMVVPLREPDDPLVKIVGAQSVRVNVL
                     LTPFTVTRLLSRVAVEVEGKSVYPGWKVEPPSVNVTVEGVPSEVNSLDEKELLIQPFV
                     NVTNVVSRRLTVPVQIRNRTEGKLKVVRVEPSNITVIADVQ"
     gene            638..2002
                     /gene="glmM"
                     /locus_tag="P5219_05210"
     CDS             638..2002
                     /gene="glmM"
                     /locus_tag="P5219_05210"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRX26187.1"
                     /translation="MTSNPERVRCLFGTDGVRDVANRGDMTPEMALRLGRSYILFLTE
                     RGVPRPRIAVGRDTRRSGQMLEAALAAGMTSAGAEVFLLGVIPTPGVSFAVKKTGMHG
                     GAVISASHNPAEYNGIKFLDGEGYKLSDEMEAAIEEYMGDNLTDDWRPTGASVGVMRQ
                     TPELAWEYGDWLASLWGRELPFPVKGAVDCAHGAASFVAPPLFEKLGGMWTVVGNEPD
                     GLNINEGVGVMKMSHICGLVQEKGLPVGIAYDGDADRVLLSDGRGRPLDGDIMLWILG
                     RWLASAGCLGSGVVATVMSNMALEEHLGRENIRVHRCQVGDRYVMETMNAAGSRLGGE
                     QSGHVIISSVVNTGDGICTGLYFLKACLALGEDMDTLADRFSPYPQILRNVEISREKR
                     VNGDFIKELSSDVESLLGGTGRILIRPSGTEPLMRVLVEARDRKLMEEVSNMLVNAIR
                     EKCM"
     gene            2036..>2522
                     /locus_tag="P5219_05215"
     CDS             2036..>2522
                     /locus_tag="P5219_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRX26188.1"
                     /translation="MPSSIKKCLFPVAGLGTRFLPATKEIAKEMLPLVDRPIIAYGVE
                     EALAAGCREMVMITGRAKKAIEDYFDRSFELEEMLKARNKRTLYDEVISLSELADFIY
                     LRQHRPLGLGHAVLCGEPVCRNEYFGVILPDDVMIARPSVLAQLIAVHEERGGSVIAL
                     EE"
BASE COUNT          548 a          694 c          781 g          499 t
ORIGIN      
        1 gagccgaagc gggtgaaatt cagagccgtt ctcgcggaag gagtgccgaa gaaaaacgtt
       61 ccggtgaacg cgaggattat cggcaagccg gtggatgatt tccgtctgaa agccgtggtg
      121 gtggagccgg cggtggtcac cgtcgaaggt cctctggcga agctcgaccg ggtgacgaag
      181 cttgataccg aaaccattga catcaccgac atcaccaaag agcagaacat ggtcgtccct
      241 ctcagggagc ctgacgaccc gctggtgaag atcgtcgggg cccagtccgt gagggtgaac
      301 gtccttctga cgccctttac ggtgacccgg ctcctgtcca gggtggctgt cgaggtggag
      361 ggaaagagcg tctaccccgg gtggaaggtg gaacctccct cggtgaatgt caccgtggaa
      421 ggagttccct cggaagtcaa ttccctggat gaaaaggaac tcctcatcca gccctttgtc
      481 aacgtgacca acgtcgtttc ccgccgtttg accgttccgg ttcagatccg gaacaggact
      541 gaagggaaac tgaaggtggt cagggttgaa ccctctaata ttacggtcat tgcagacgta
      601 cagtgaatat ttctaaccgc tgggatggtg aggcaacatg acgagcaacc ccgaaagagt
      661 gcgctgcctt ttcggaactg acggcgtccg ggatgtcgcc aacaggggag acatgactcc
      721 ggaaatggcc ctgcgcctcg ggcgttccta tattctcttc ctcacggaaa ggggagttcc
      781 gaggccccga atcgccgtgg gaagggatac gaggagatcg ggacagatgc tggaggccgc
      841 ccttgccgcc ggtatgacct ccgccggggc ggaagtgttc ctgctcggag tgattccgac
      901 acccggcgtc agttttgctg tcaagaagac cggcatgcac ggcggagcgg tgatcagcgc
      961 ctcccacaat ccagcggagt acaacggaat caagttcctc gacggtgagg gctacaagct
     1021 ctccgatgaa atggaggccg ccatcgagga atacatggga gataacctca cagacgactg
     1081 gcgtcccaca ggcgcgtcgg tcggcgtaat gcgtcagact cctgaacttg cctgggaata
     1141 cggcgactgg cttgcctcac tctggggcag ggagctgcct ttccctgtca aaggcgcggt
     1201 ggactgcgcc cacggcgccg catccttcgt ggctcctcct cttttcgaga agctcggcgg
     1261 catgtggacc gtggtgggaa acgaaccgga cggcctgaac ataaacgaag gcgtgggcgt
     1321 gatgaaaatg tcccatatct gcggcctcgt tcaggaaaaa gggctgcctg tggggatcgc
     1381 ctacgacgga gacgccgacc gggttctcct gtccgacgga agaggcaggc cccttgacgg
     1441 agacattatg ctgtggattc tcggccgctg gctcgcctcg gcgggatgtc tcggctcggg
     1501 ggtcgtggcc acggtgatga gcaacatggc cctggaagaa catctgggaa gggagaacat
     1561 acgggttcac cggtgccagg ttggggacag atacgtcatg gagaccatga atgcggcggg
     1621 atcccgcctg ggaggagagc agtcgggcca cgtgatcatc tcctcggtgg tcaacaccgg
     1681 agacggcatc tgcacgggcc tctatttcct gaaggcgtgc cttgctctgg gcgaagatat
     1741 ggacaccctt gccgacaggt tttctcccta cccccagatt ctccggaacg tggaaatttc
     1801 gagggagaag cgagtgaacg gcgattttat aaaagaactt tcttccgacg tggaatcgct
     1861 tcttggggga accggccgta ttctcatccg gccgtccggc accgaacccc ttatgagagt
     1921 tctcgtggaa gccagggaca gaaagctcat ggaagaagtc tcaaacatgc ttgtgaacgc
     1981 cattcgcgag aaatgcatgt aaatatttct gcctacagga aaggtgaagg attccatgcc
     2041 ctcgtccatt aaaaaatgtc ttttccctgt tgccggtctt ggaacccgct tcctgccggc
     2101 aaccaaggag atcgccaagg aaatgcttcc cctggtggac cggcccatca tcgcctacgg
     2161 cgtcgaggag gctcttgccg caggatgccg tgaaatggtc atgattaccg gcagagccaa
     2221 gaaggccata gaagactact ttgaccgatc cttcgaactc gaggaaatgc ttaaggccag
     2281 aaacaagaga accctctacg acgaggtgat ctctctctcg gaactggccg atttcatcta
     2341 cctcaggcag caccgtcccc tgggactggg gcatgcagtg ctctgcggag agccggtgtg
     2401 ccggaacgag tatttcggcg tcattcttcc cgatgatgtc atgatcgccc gtccttcggt
     2461 cctcgcccag ctcatcgccg tccatgaaga acgggggggc agcgtcatcg ccctggagga
     2521 ag
//
LOCUS       DAOTHD010000249         2512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_49264_length_2512_cov_3.086284, whole genome shotgun sequence.
ACCESSION   DAOTHD010000249 DAOTHD010000000
VERSION     DAOTHD010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49264_length_2512_cov_3.086284"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(303..1451)
                     /gene="grdD"
                     /locus_tag="P5219_05220"
     CDS             complement(303..1451)
                     /gene="grdD"
                     /locus_tag="P5219_05220"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit alpha"
                     /protein_id="HRX26189.1"
                     /translation="MSEIRKLIGEALAEIVSDARSGGPRISVGLMAMGSELGAEELAR
                     GARMARENSSRLEVVMIGPKIPGYDDLAWIETPDCEEDVAKAMEKALSDGRIAGAVAL
                     HYPFPLGVTTIGRVLTPGRGKPMILASSTGTAAVGRVEAMVRNALYGIATAKAIGLES
                     PTVGILNVDGAQLAYKALNSLKEKGYPISFGASVRKDGGAVLRGNDILAGAVDVCVTD
                     TLTGNVLMKMFSSFTTGGSYESLGWGYGPSAGEGWGKIISIISRASGAPVIAGALEYT
                     ARAASGGLPEKVAAELDSARKAGLDNLLEGLAPKAPCADQVTAPPAEPTDEEIHGVDV
                     LSIEDGVKVLWKEGIYAESSMGCTGPVIKVPAKHLDRAEEVLRTNGYI"
     gene            complement(1468..>2512)
                     /gene="grdC"
                     /locus_tag="P5219_05225"
     CDS             complement(1468..>2512)
                     /gene="grdC"
                     /locus_tag="P5219_05225"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit beta"
                     /protein_id="HRX26190.1"
                     /translation="LYCGGEIVGLVRKGHDVDECLSAHVLMENLASKAGAVLSLLHLL
                     KNTGMAASEVDFVIECSEEGAGDMCQRAGGNFAKAIAESAGCINASGCDVRGFCAGPV
                     NAMIAGATQVASGARKNCVVLAGGAIPKLYMNGRDHVKKKMPTLEDCLGNFAVLLVPD
                     DGTNPVLRLDVLGKHTVGAGSSPQAVTTALILDPLERAGLSFMDVDKYAAELHINEIT
                     LPAGAGDVPLANIKMTAALAVTKKAIEKADMMTFVKERGVPGFAHTQGHIPSGVPFIG
                     HACDALKDGSMKRAMIIGKGSLFLARLTNLADGASFLLEPPSGTGAAGAAVSKDDIKA
                     LLLEVLSELSGKL"
BASE COUNT          456 a          770 c          777 g          509 t
ORIGIN      
        1 catccagggc ccccatacga ttccgttgac aatcccgttg attctcatta cttcctgcat
       61 agcgtgcgat tccccctctc atgttattca gcgcggatac ttcctcccct ccgggctctc
      121 ccgggatggg ggtgcctcaa aaacagaaaa aacggtggaa tttgaaacag ggtgcagatt
      181 gaagttcggc cttcaccccc tttaatgtcg gaataggttc attctttcag aaaaagtata
      241 gcacagatca cttcttttga aagagaaagg ggaagctcaa tcaagcttcc cccctgctaa
      301 tgtcaaatat atccgttggt cctgagaact tcctcagccc tgtcgaggtg ttttgcggga
      361 acctttatca cggggccggt gcatcccatg gaggactccg catagatccc ctctttccag
      421 aggaccttca cgccgtcctc aatggacagg acatcgacac cgtggatctc ctcgtcggtg
      481 ggttcggcgg gaggagcggt tacctggtcg gcgcacggag ctttcggagc cagcccctcg
      541 agcaggttgt caagaccggc tttccgggcc gagtcgagct ccgccgccac cttttcaggc
      601 agtccgccgg aggcggccct ggcggtgtac tccagggcac ctgcgatgac cggcgctccg
      661 gaggccctgg agatgatgga gatgattttg ccccaccctt cccccgccga gggaccatag
      721 ccccatccca gggattcata ggatcctccg gtggtgaagg aggagaacat cttcatcagg
      781 acattccccg tcagggtgtc ggtgacgcag acgtccaccg ctccggcgag aatgtcgttc
      841 ccccggagaa ccgcccctcc gtccttccgg acgctggcac cgaaagaaat gggatatccc
      901 ttttccttca gggaattgag ggccttgtag gcaagctggg cgccgtccac gttgaggatt
      961 cccaccgtgg ggctctccag gccgatggct tttgcggtcg cgatgccgta cagggcgttc
     1021 cggaccatgg cttcgaccct gccgaccgcc gcggtgcccg tggacgaggc aagaatcatg
     1081 ggcttgcccc tgcccggagt caggacccgg ccgatggtgg tgacgccgag agggaaggga
     1141 taatgaaggg cgaccgctcc ggcgatcctg ccgtcggaaa gggccttctc catggctttc
     1201 gcaacgtctt cctcgcagtc cggagtttcg atccatgcca ggtcgtcata gccgggaatt
     1261 ttcggaccga tcatgaccac ttcgagccgg gaggaatttt cccgggccat ccgcgctccc
     1321 ctggcgagtt cttccgcccc gagttcactg cccatggcca tgagtccgac ggagatccgc
     1381 ggtccgcccg accgggcgtc ggacacgatc tcggccaggg cttcaccaat gagcttccgt
     1441 atttcggaca ttgtcgctca cctccctcta cagctttccg gacagttcag agagaacctc
     1501 caggagaaga gccttgatat cgtccttgga gaccgccgct ccggccgccc cggtgccgga
     1561 ggggggctcg agaaggaagg aggctccgtc ggcgagattc gtgagcctgg cgaggaagag
     1621 gcttcccttg ccgatgatca tggctcgttt catggagccg tccttcaggg cgtcgcaggc
     1681 atggccgatg aaggggactc ccgaagggat gtgcccctgg gtgtgggcaa accccggaac
     1741 gccccgctcc ttgacgaagg tcatcatgtc cgccttctct attgccttct tcgtaacggc
     1801 aagggcggcg gtcatcttga tgttcgccag gggcacgtcg ccggcgcccg caggaagggt
     1861 gatttcgttg atatgaagct ccgcggcgta cttgtccacg tccatgaagg aaagtccggc
     1921 ccgctcaagg gggtcgagaa tgagggcggt ggtgacggcc tggggcgaag accccgcacc
     1981 gacggtatgt tttccgagga cgtcgagccg cagaacgggg ttcgtcccgt cgtcgggaac
     2041 aagcaggacg gcgaagtttc cgaggcagtc ctcgagggtg ggcatctttt tcttgacgtg
     2101 gtcccggccg ttcatataga gcttcggtat ggcgccgccc gccagtacga cgcagttctt
     2161 ccgtgcgccg gaggccacct gggttgcacc ggctatcatg gcgttgacgg gaccggcgca
     2221 gaatccccgg acgtcgcagc cgctggcgtt gatgcagcct gcagattcag cgatggcctt
     2281 ggcgaagttg ccgcccgccc gctggcacat gtcgccggca ccctcttcgg aacattcgat
     2341 gacgaagtcc acttccgagg cggccatgcc ggtgtttttc aggagatgga gaagggagag
     2401 gactgcaccg gccttggagg cgaggttctc catcaggacg tgagcggaca ggcattcgtc
     2461 cacgtcgtgt cccttgcgga ccaggccgac gatctccccg ccgcagtaga gg
//
LOCUS       DAOTHD010000250         2507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_49388_length_2507_cov_3.818108, whole genome shotgun sequence.
ACCESSION   DAOTHD010000250 DAOTHD010000000
VERSION     DAOTHD010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49388_length_2507_cov_3.818108"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="P5219_05230"
     CDS             complement(<1..441)
                     /locus_tag="P5219_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26191.1"
                     /translation="MMKKTAVLVFLLLFLAGAGEGAGWYNMYTGGVWNNPGSCLIDTM
                     IYNKTMANAALKNMQKNQSQAARPQKKPQQVPQARPTTYRPSGKPSPVPAALARTLTD
                     RKADRDTMTAFFKEALANYGDYARQAKRPNDLGLAMAFFFGVCIG"
     gene            complement(505..1026)
                     /locus_tag="P5219_05235"
     CDS             complement(505..1026)
                     /locus_tag="P5219_05235"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRX26192.1"
                     /translation="MPRVYYSTIISAPIEEVWEYVRAFNALPTWHPGIKASEIEGGCC
                     PDCTACVGSVRHFQLADDSWMREQLLALSDADYSVTYTILETGMKLKNYVASLSLAPV
                     TATDETFAEWEAVFDATDPAAEEETVDAVYGVFSSGLENLDEMLMEEFDIDSCGECGE
                     GECGGCGCGCGEK"
     gene            complement(1067..1897)
                     /locus_tag="P5219_05240"
     CDS             complement(1067..1897)
                     /locus_tag="P5219_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HRX26193.1"
                     /translation="MKRFTRTELLARFHDMAKKKEPIIGGGAGTGISAKWEEAGGIDL
                     IVIYNSGRYRMAGRGSLAGLLAYGNANDIVKEMAYEVLPVVKKTPVLAGINGTDPFVI
                     WDKYFDELISLGFAGVQNFPTVGLIDGVFRANLEETGMGYALEVEAIRAAHERDLLTT
                     PYVFSEEDAVAMTKAGADILVCHMGLTTKGSIGAKTAKTLDECAVLIEKWAKAAKKVR
                     KDVIVLCHGGPIAMPEDASFILKKCKNVHGFYGASSMERLPTETAIKAQTEEFKKITF
                     "
     gene            complement(1922..>2507)
                     /locus_tag="P5219_05245"
     CDS             complement(1922..>2507)
                     /locus_tag="P5219_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HRX26194.1"
                     /translation="DFDCLVFHATGTGGQSFEKLVDSGMMKCVLDITTTEICDHLMGG
                     VLSAGEGRLDAFIRTGIPYVGSVGALDMVNFGAMDTVPEKYKGRKLYVHNPQVTLMRT
                     TPEENTRMGEWIGDKLNRFPGPVRFLLPLKGVSMIDAPGQAFHWPEADEALFSALERT
                     FRQSDQHRLVKLPLNVNDPEFAAALVENFRELHP"
BASE COUNT          389 a          731 c          834 g          553 t
ORIGIN      
        1 gccgatgcac accccgaaga agaaggccat ggcgagccca agatcgttgg ggcgctttgc
       61 ctgccgtgca tagtccccgt agttcgccag agcctctttg aagaaggcgg tcatggtgtc
      121 cctgtcggcc tttctgtcgg tgagggtccg ggcgagggcg gccggcacgg gggacggctt
      181 tcctgacggg cggtaggtgg tcgggcgggc ctggggaacc tgctggggct tcttctgggg
      241 ccgggccgcc tgggactggt ttttctgcat gttcttgagg gcggcgttgg ccatcgtctt
      301 gttgtagatc atggtgtcga taaggcagga tccggggttg ttccagaccc ctcccgtgta
      361 catgttgtac cagcccgcgc cttctccggc gccggcaagg aaaagaagca ggaaaacgag
      421 cactgccgtt tttttcatca tgagcccatc cccttttgaa gaagaaaggg ccggacgcat
      481 ctgtccggcc cggtcagcat caggctattt ctctccgcag ccgcatccgc agccgccgca
      541 ttccccttcg ccgcattcgc cgcaggaatc gatgtcgaat tcctccatga gcatttcgtc
      601 caggttttca aggcctgaac tgaagacccc gtagaccgcg tccaccgtct cttcctccgc
      661 tgcgggatcg gtggcgtcga agacggcctc ccattcggcg aaggtttcat cggtggcggt
      721 gaccggtgcg aggctcaggg atgccacgta gttcttcagc ttcatgcccg tttcgaggat
      781 ggtgtaggtg acggaataat ctgcgtcgga gagggcgaga agctgttccc tcatccagga
      841 gtcatcggcg agctggaaat ggcggacgct gcccacgcag gcggtgcagt cggggcagca
      901 gcctccctct atttcgctgg ccttgatccc cgggtgccac gtgggcaggg cgttgaaggc
      961 cctcacgtat tcccatactt cctcgatggg ggcggagatg atggtgctgt aatacactct
     1021 gggcatggcg ttccctcctt ttctgtctgt ggtgccgctt cggttcctag aaagtgatct
     1081 tcttgaactc ttccgtctgg gccttgatgg ccgtttccgt gggcaggcgc tccatggagc
     1141 tggcgccgta gaacccgtgg acgttcttgc atttcttgag gatgaaggac gcgtcttccg
     1201 gcatggcgat ggggccgccg tggcagagga cgatcacgtc cttgcggacc tttttggccg
     1261 ccttcgccca tttttcgatg aggacggcgc attcgtcgag ggttttggcg gtcttcgcgc
     1321 cgatggatcc ctttgtggtg agccccatgt ggcacacgag gatgtccgct ccggccttcg
     1381 tcatggccac ggcgtcctct tcgctgaaga cgtagggggt ggtgaggagg tcccgctcgt
     1441 gggcggcccg aatggcctcc acttcaaggg cgtaccccat gccggtctct tccaggttgg
     1501 cccggaagac cccgtcgatg agccccacgg tggggaagtt ctgcacgccg gcgaagccga
     1561 gggagatcag ctcgtcgaag tatttgtccc agatgacgaa ggggtcggtg ccgttgatgc
     1621 cggccaggac gggcgttttc ttcactacgg ggaggacttc gtaggccatc tccttgacga
     1681 tgtcgttggc gttgccgtag gcgaggagtc ccgcgaggga cccccgtccc gccatgcggt
     1741 agcggccgga gttgtagatg acgatgaggt cgatgccgcc ggcctcttcc cacttggcgg
     1801 atatgcctgt gcctgctccg ccgccgatga tgggctcctt ctttttcgcc atgtcgtgga
     1861 agcgggccag aagttcggta cgggtgaacc gtttcatgaa atcccttccc ttccctttgg
     1921 gttatggatg cagttcccgg aagttctcca cgagggccgc ggcgaactcg ggatcgttca
     1981 cgttcagggg cagtttcacc aggcggtgct ggtccgactg tctgaaggtc cgctcgaggg
     2041 cggagaaaag ggcttcgtcc gcctcaggcc agtggaaggc ctgccccggc gcgtcgatca
     2101 tggagacgcc cttcaggggc agcaggaagc gcacgggccc ggggaacctg ttcagcttgt
     2161 cgccgatcca ttcgcccatg cgggtgtttt cctcgggggt ggtgcgcatg agggtcacct
     2221 gggggttgtg gacatagagc ttcctgccct tgtacttctc gggcacggtg tccatggccc
     2281 cgaagttcac catgtccagg gcgcccacgg agcccacgta ggggatcccg gtccggatga
     2341 aggcgtcgag ccggccttct cccgcggaga ggacgccgcc catgaggtgg tcgcagattt
     2401 ccgtggtggt gatgtcaagg acgcacttca tcatccccga gtccacgagt ttttcgaagg
     2461 actggccgcc ggttccggtg gcgtggaaga cgaggcagtc gaagtcc
//
LOCUS       DAOTHD010000251         2506 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_49433_length_2506_cov_2.414933, whole genome shotgun sequence.
ACCESSION   DAOTHD010000251 DAOTHD010000000
VERSION     DAOTHD010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49433_length_2506_cov_2.414933"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2506
                     /locus_tag="P5219_05250"
     CDS             <1..>2506
                     /locus_tag="P5219_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRX26195.1"
                     /translation="SPENDGAGTVNSRTLQAHDRFSLQVAWDGNRASDASRYQGSASA
                     WLWEGGTAGGGTSGQNAAALSGALGLPGFFTGFVAADGDAHGPDLVAGDSWMTYTTAR
                     VAGTADRLELSLYDGLYADGGNNGGVTEGIVYAFDDGALDDRSISINQLVRTAPGIGG
                     SENLSHRLDFGSVSQNTTLSYGERGSAGTYWENTGGSPSWYSHSYYAGDTTCFFGSGG
                     SPSDIVRNAEVYRQNDINASLLFEFDGARLTVRAKGFDRNGSELPESADTSPWSFPPA
                     VFSQLASGADVTLFQGTSHEIRCTNLQVATDRLSAGDKFVINVAAAAKLDGLNTSPSL
                     SGGFLSAANVAVEGDPFRVQSGEWGSSAQYRLADGAENGADLNLLGYFVDPLNGRSDV
                     PGLGYYTGTLILQAEAAGFAPGSTVGAPDGTDAGSRRVRAEINYQGDARPSAGALVTS
                     AFFGDMEAGGETDLKRYIGGVGYSNYLYGRRGDGTYGPLNEPGAERYNPYNASLVFDV
                     ISVENGSMRFRVQGHVIGRDGRQWYAEEEDFGLNDGQNTAKNSAALPPVIPSEVVNPV
                     VLFRDSEFGGLFFDEFTLGDSSLWNEGDRFTLSLAASGFTDGISEADQLSDPLLSPAS
                     DELDLFSDNRGTNMPHSLRFNEGVLDNARVDIGIYQVANNLARRGSGAFAKDQVMDGT
                     LSLSFSDFHPSSADVEENALTFESVFGSGMDAGPAHYYSRLSDIGLFYDGNGRFLLEG
                     DGGRLTIRQGERETHVTVGAGMEVGQMAEYISRQIWLDLLGQEDGIPRGEGSRFPEGS
                     SPRLMDRRDMNRIFRFIQHVPGQSGNDSVVSTLLAHS"
BASE COUNT          461 a          762 c          836 g          447 t
ORIGIN      
        1 ggtccccgga gaacgacggc gccggtacgg tgaactcccg gacgctccag gcccatgacc
       61 gtttctcgct gcaggtggcc tgggacggca acagggcctc cgacgcatcc cggtaccagg
      121 ggtcggcctc cgcctggctt tgggagggcg gaaccgcggg aggagggacg tcggggcaga
      181 atgccgccgc cctgagcgga gctctcgggc ttcccggctt cttcaccggc tttgtcgccg
      241 cggacgggga tgctcacgga ccggacctcg tcgccgggga ttcctggatg acctacacca
      301 cagcccgggt tgcaggcact gcggacaggc tcgagctctc cctttacgac ggactgtacg
      361 ccgacggcgg gaacaacggc ggcgtcacgg agggtattgt gtatgccttc gacgacggag
      421 cgctggacga ccggtccatt tccatcaacc agctcgttcg gaccgcaccc ggaatcgggg
      481 gctcggagaa tctttcccac cggcttgatt tcggctccgt gagccagaat accaccctct
      541 cctacggaga gcgggggagt gccgggacct actgggagaa cacgggaggc tccccgtcat
      601 ggtactccca cagctactat gccggggata cgacctgttt cttcgggagc ggcggcagcc
      661 cttccgatat tgtccgaaac gccgaggtct accggcagaa cgatatcaac gcgtcccttc
      721 tgttcgaatt cgacggggcg cgactgacag tgcgggcaaa gggcttcgac aggaacggca
      781 gcgagctgcc cgagtcggcc gacacctccc cctggagctt ccctccggcc gttttttccc
      841 agcttgcttc tggcgcggac gtaaccctct tccagggcac ttcccacgaa atccgctgta
      901 cgaacctcca ggtggccacg gaccggcttt cggcagggga taaattcgtc atcaatgtcg
      961 ccgccgccgc caagctggat gggctgaata cgtcgccttc actttcgggg ggcttcctgt
     1021 cggcggcgaa cgtcgccgtc gaaggcgacc ccttccgggt gcagtccgga gaatggggca
     1081 gttccgccca gtacaggctc gcggacggag cggagaacgg cgccgatctc aatctcctgg
     1141 ggtattttgt cgatcccctg aacggcagga gcgacgttcc cgggctcggc tactacaccg
     1201 ggacgctcat ccttcaggcg gaagcggcgg gattcgctcc gggaagcacc gtgggggcgc
     1261 cggacggcac tgatgcgggt tcccgccggg tccgggcgga gatcaactac cagggagacg
     1321 ccagaccttc tgccggagcc cttgtgacga gcgccttttt cggggacatg gaagccgggg
     1381 gagagacgga cctcaagcgg tacatcgggg gagtggggta ctcgaactac ctctacggca
     1441 ggcggggcga cgggacctac ggcccgctga atgaaccggg tgccgagcgg tacaatccgt
     1501 acaacgcctc gctggtattt gacgtgatca gcgtggaaaa cggctccatg aggttccggg
     1561 tccaggggca tgtcatcggc agggacggaa ggcagtggta tgcggaagag gaagatttcg
     1621 ggctcaacga cgggcagaac accgcaaaaa acagcgcggc gctgcccccg gtgatcccgt
     1681 ccgaggtggt gaacccggtg gtcctttttc gggactcgga gttcggcggg ctgtttttcg
     1741 acgagttcac cctcggtgac agttccctgt ggaacgaagg ggacaggttt accctctcgc
     1801 tggctgcgag cgggttcacc gacggaatca gcgaagcgga ccagctgagc gaccctctcc
     1861 tgagccccgc ctccgatgaa ctcgacctct tctccgacaa caggggaacg aacatgcccc
     1921 attccctgag gttcaacgaa ggcgtcctgg ataatgcccg ggtggatatc gggatctacc
     1981 aggttgcgaa taacctcgcc cggaggggga gcggcgcttt tgcgaaggac caggtcatgg
     2041 acggaacgct gtccctttct ttcagcgatt tccatccctc gtcagcggat gtggaggaga
     2101 acgccctgac ctttgagtcg gtcttcggga gcgggatgga cgcggggcct gcccattact
     2161 acagcaggct gtccgatatc ggcctgtttt atgacgggaa cgggcgcttc ctcctggaag
     2221 gggacggcgg gaggctgacc atccgccagg gagagaggga aacccatgtc accgtcgggg
     2281 ccggcatgga agtggggcag atggccgaat acatctcccg gcagatctgg cttgacctcc
     2341 tcgggcagga ggacggcatc cccaggggag agggcagccg ttttcccgaa ggcagcagtc
     2401 cccgcctgat ggacaggagg gacatgaaca ggattttcag gttcatacag catgtgccgg
     2461 ggcaaagcgg caatgattcg gtggtttcaa cccttctcgc ccattc
//
LOCUS       DAOTHD010000252         2505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_49449_length_2505_cov_3.322857, whole genome shotgun sequence.
ACCESSION   DAOTHD010000252 DAOTHD010000000
VERSION     DAOTHD010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49449_length_2505_cov_3.322857"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..1332)
                     /gene="rodA"
                     /locus_tag="P5219_05255"
     CDS             complement(226..1332)
                     /gene="rodA"
                     /locus_tag="P5219_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRX26196.1"
                     /translation="MEEKRLSLRENLASLDRLQFLSVLALFFLGVASIFSAGAGFDGR
                     GSGFAARQLLWGCLSAVAYLGVLQVGYARFLSWAYRIYAFALVLLFGVMVLGMTVKGS
                     QSWFHLGFFRFQPAEFGKIALILVLAKHFCRYPPLTLPSFLGGLALAGISGWLVLVQP
                     DAGSAMVYGAIAMVTVAVAGAPWKYPAGLIGLMMAAVPLGWQFLKEYQKMRIRVFLDP
                     WVDPLGAGYNVIQSRIAVGSGGLLGKGFMEGLQSKLRFLPEPHTDFIFSVYCEEFGFI
                     GAVGMLLLFGVLFWRLIETAMRTKDSRAKVLIAGISAWIWFQMAESIGMSMGLLPVTG
                     LPLPLVSYGGSSLLALSVALALAQSVYISTLKTY"
     gene            complement(1283..1624)
                     /gene="minE"
                     /locus_tag="P5219_05260"
     CDS             complement(1283..1624)
                     /gene="minE"
                     /locus_tag="P5219_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HRX26197.1"
                     /translation="MALEFLKKFFGSGGSKNIAKERLQIVLIHDRADISPEMLEQLRR
                     DMIDVITRYMDIDTSKIEMDFDRADRSVAFVANIPVIRIKRGVAPGKMEQEIPDGGKK
                     AQPKRKPGQPG"
     gene            complement(1637..2443)
                     /gene="minD"
                     /locus_tag="P5219_05265"
     CDS             complement(1637..2443)
                     /gene="minD"
                     /locus_tag="P5219_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HRX26198.1"
                     /translation="MDPRVIVITSGKGGVGKTTTTSNISVALARAGHKVVAVDADIGL
                     RNLDVVMGLENRVVYNFIDVLEGTCRLNQALVRDKRVDNLFLLPAAQTRTKDAVSADQ
                     MEELCGMLRKEFDFILLDSPAGIESGFRNASIGADEALVITTPEVPAVRDADRIIGLL
                     ESMGKSPIRLVINRLRSRLVKQGEMLDVRDVLDILAIDLVGIVPEDDSVVKSANRGEP
                     LTFGDSAPAAQAFQRIASRILGETVPFPDFSEEQGRGFFAGIRKFFGRKE"
BASE COUNT          536 a          780 c          721 g          468 t
ORIGIN      
        1 cccggaatcc tccttcgggg gcagggagcc gaattcacat ccggcatacc gggaaggccg
       61 cttcacatcc gaaagaagcg gccacattcc atcggcatat tcaggaaaat gcatacagcg
      121 cctcctagaa aaatccggcg tccccggcgc aggccacggg acgtccggtc caaaataccc
      181 gaacggcacg gaagaacgcc tccgtctcct tcgggttcat tctcgctaat aggttttcag
      241 cgtggatata tacacgctct gggcaagggc cagggcaacg gaaagggcga gcagggagct
      301 tccgccgtag ctcaccagcg gcagggggag tcccgtgacg ggaaggagtc ccatgctcat
      361 gccgatgctc tccgccatct ggaaccagat ccaggcggaa atgccggcga tgagcacttt
      421 cgcccggctg tccttggtgc gcatggccgt ctcgatgagc ctccagaaga gtaccccaaa
      481 gagaaggagc atgccgacgg ccccgataaa gccgaattct tcgcagtaga cgctgaaaat
      541 aaagtcggtg tggggttccg gcagaaacct gagtttgctc tgcagcccct ccatgaaccc
      601 tttgcccagg agtcctcccg atcctacggc aatcctggac tggatcacgt tgtatcccgc
      661 ccccagggga tccacccacg gatcgagaaa aacccggatc cgcattttct ggtactcctt
      721 caggaactgc cacccgaggg gcaccgccgc catcatcagg ccgatgaggc ctgccgggta
      781 tttccacggg gcgccggcaa ccgccacggt caccatggcg attgccccgt agaccatggc
      841 gcttccggcg tcgggctgga cgagcacaag ccagcctgaa atccccgcca gggcgagacc
      901 tcccagaaag gaaggcagcg tcagaggagg ataccggcag aaatgcttcg ccagcaccag
      961 gatgagggca attttgccga actccgccgg ctggaaccgg aaaaagccca ggtgaaacca
     1021 gctctgggat cccttcaccg tcattccgag caccatgacc ccgaaaagaa ggacaagggc
     1081 aaaagcatag atacggtaag cccaggagag aaaccgggca tacccgacct ggagcacccc
     1141 gagataggcg acagcggaga ggcagcccca caggagctgc ctggccgcaa atccgctccc
     1201 tctgccgtcg aaaccggcgc ccgcgctgaa aatggacgcc acgccgagaa agaagagggc
     1261 caggaccgaa agaaactgga gcctatccag gctggccagg ttttctctta ggctgagcct
     1321 tttttcctcc atcaggaatc tcctgttcca tctttccggg agcaaccccc cgcttgatcc
     1381 gaatgaccgg gatgttggcc acgaaggcca ccgatctgtc agcccggtcg aaatccatct
     1441 ctatcttgga cgtgtcgatg tccatgtagc gggtgatcac gtcgatcatg tcccggcgga
     1501 gctgttcgag catctcgggc gaaatgtccg cccggtcgtg gatgagaacg atctgcaggc
     1561 gctccttggc gatgttcttc gaaccgccgg agccgaaaaa tttcttcaga aattcaagtg
     1621 ccatggcgcc atccccctat tcctttctgc cgaagaactt ccggattccc gcgaaaaatc
     1681 ctcttccctg ttcttcgctg aagtcgggga atggaacggt ttctccgagt attcgggagg
     1741 cgatgcgctg gaacgcctgg gccgccgggg cggagtcgcc gaaggtgagg ggctctcccc
     1801 tgttcgcgga tttcaccacg ctgtcgtcct cggggacgat gcccacgaga tcgatggcga
     1861 gaatgtccag tacgtcccgc acgtcgagca tttcgccttg cttgaccagc cgggaacgga
     1921 gcctgttgat cacaaggcgg ataggggatt tgcccatgga ttcaaggagg ccgatgatcc
     1981 tgtcggcgtc ccgaacggcg ggaacctccg gcgtggtgat gacaagggcc tcgtccgccc
     2041 cgatggaggc gttgcggaac cccgactcga taccggctgg gctgtcgaga agaatgaagt
     2101 cgaattcctt gcggagcatg ccgcacagtt cttccatctg gtcggcgctc acggcgtcct
     2161 tggtcctggt ctgggcggcc ggcagcagaa aaaggttgtc caccctcttg tccctgacaa
     2221 gagcctggtt gagcctgcag gttccttcca gcacgtcgat gaagttgtag accaccctgt
     2281 tttccaggcc catcacaacg tcaagattcc gcagcccgat gtcggcgtcc acagccacaa
     2341 ccttatgtcc ggcccgtgca agggccacag aaatattcga tgtggtggtg gtttttccga
     2401 caccgccttt tcccgaagtt atcacaatca cccgagggtc cactgaattt ctccccttcc
     2461 cttccccggc agccggattt ctcccgtccg gggaaatggt ctcat
//
LOCUS       DAOTHD010000253         2503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_49479_length_2503_cov_3.352124, whole genome shotgun sequence.
ACCESSION   DAOTHD010000253 DAOTHD010000000
VERSION     DAOTHD010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49479_length_2503_cov_3.352124"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="P5219_05270"
     CDS             <1..458
                     /locus_tag="P5219_05270"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRX26199.1"
                     /translation="CVKACTQKVTGALKMKDGKVVKDDRRCIGCGECVLACPTGAWTR
                     RPGSLYRLLIMGRTGKKNPRLAMPFLDRVTEDVILRVIRNTLDYVDAHILRNLVKEHI
                     GYIVDRTGYPVFRDAVLKGVTLNPEARVAKTMSWPGYHTLDDGNLKDVE"
     gene            485..1219
                     /locus_tag="P5219_05275"
     CDS             485..1219
                     /locus_tag="P5219_05275"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HRX26200.1"
                     /translation="MFEASYSIPYAGIGPDGRMKLPVLLDILQDMADRDARRMSMTVS
                     DLLPKGISWVLRRYTVSVERYPEYESTLTVKTWHGPRRNLHSMRAFSVWDERGPVASA
                     DTSWILIDLERGRPLRLDRHATPRYVEESLPVTAEFPDLPCPEEWEDERPFSVRRWDL
                     DRNNHVNNAVYLSWAAEAVPEETAKDCSLVRVDAEYLRSTEGRGDIRVRTARPGVQGA
                     EISYVHSIVGPKGDEKARVITLWRKG"
     gene            1306..1710
                     /locus_tag="P5219_05280"
     CDS             1306..1710
                     /locus_tag="P5219_05280"
                     /inference="COORDINATES: protein motif:HMM:NF025589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HRX26201.1"
                     /translation="MANLEKVEGIGETYAKKLRDAGVGSTDMLLETGSTRKGRDELAA
                     KTGIASKLILEWVNHVDLFRVKGVGEEYADLLEASGVDTVPELAQRRADNLHKKMAEV
                     NAEKKLVRQLPSEKQVQEWIEQAKELPRKIQY"
     gene            1925..>2503
                     /locus_tag="P5219_05285"
     CDS             1925..>2503
                     /locus_tag="P5219_05285"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HRX26202.1"
                     /translation="MRIRAINAEKMGLSNRLTLINLIRTNPGVSRRELSRLAGLDPST
                     VTKIITSLLDKGLVVEQGVRDADQPGRKSIMLRVSRDAAVCLAAEVGVESTRIAAGYL
                     DASFEVLEEFSTPPDQDSFFRVFADRMESIRSSSKTREFCALSFAVPGMVEWGSNVLK
                     HLPHMGWKDVNLAGNLRRTLPWLDIPVFAANEA"
BASE COUNT          521 a          731 c          783 g          468 t
ORIGIN      
        1 cctgcgtgaa ggcctgtacc cagaaggtca ccggcgcgct gaagatgaag gacggcaagg
       61 tggtgaagga cgaccggagg tgcatcggct gcggcgagtg cgtcctcgcc tgccccaccg
      121 gcgcgtggac ccgccgcccc gggagcctct accggctgct catcatgggc cgcaccggaa
      181 agaaaaaccc gaggctcgcc atgcccttcc tcgacagagt caccgaagac gtgatcctca
      241 gggtcatccg gaacaccctg gattacgtcg atgcacatat tctccggaac cttgtcaagg
      301 aacatatcgg ctacatcgtg gaccgtaccg ggtatcccgt gttccgggac gccgtgctca
      361 aaggggtgac cctcaacccc gaggccaggg tcgcgaagac catgagctgg ccgggatacc
      421 atacccttga cgacgggaat ctgaaagatg tcgaataaca ttcgctgaag aaagagatga
      481 tcccatgttc gaagcgtcct attccatccc ctacgccggc ataggtcccg acggccggat
      541 gaagctcccg gtgctgctcg acatcctcca ggacatggcg gaccgggatg cccgccgcat
      601 gtccatgaca gtgtcggacc tgctcccgaa agggatcagc tgggtgctca gaaggtacac
      661 ggtctccgtg gagcggtatc ccgaatatga aagcaccctc acggtgaaga cctggcacgg
      721 gccccgcaga aaccttcatt ccatgagggc cttctccgta tgggatgaaa ggggacctgt
      781 agcgtctgcc gacaccagct ggattctcat cgatctcgag cggggaaggc ctctccggct
      841 ggaccgtcat gccacacccc gctacgtgga ggagagcctg ccggtcacgg cggaattccc
      901 tgatctgccg tgcccggaag aatgggagga cgagcgtccc ttttccgtcc gccggtggga
      961 tctggaccgg aacaaccacg tgaacaacgc ggtttactta tcctgggccg ccgaggctgt
     1021 tcccgaggag accgcgaaag actgttccct cgtgcgggtt gacgccgaat acctccgttc
     1081 cacggaggga cggggggata tccgggtccg tacggcccgg cccggcgttc agggagcgga
     1141 aatatcctat gttcattcca tcgtcggccc gaagggggac gagaaggccc gggtcattac
     1201 cctctggcga aaaggctgag tttttggaat tcccttgaca ttcccgctga tcccgctata
     1261 gttttcattg tggaatacaa cttttgaaag gaagggagac ctgtcatggc gaacctcgag
     1321 aaagtcgaag gcatcggcga aacgtacgcg aagaagctcc gggatgccgg agtaggctcc
     1381 acggacatgc ttctcgagac ggggagcacc cggaagggac gggacgagct tgccgcaaaa
     1441 acgggaatag cctcgaagct gatcctcgag tgggtgaacc acgtggacct gttccgggta
     1501 aagggcgtgg gggaggagta cgccgatctc ctggaggctt cgggtgtgga cacggtcccc
     1561 gagcttgccc agagaagggc ggataacctg cacaaaaaaa tggccgaggt caatgccgaa
     1621 aaaaaactcg tccggcagct tccgtcggaa aaacaggtgc aggagtggat tgaacaggca
     1681 aaagagcttc cccggaaaat ccagtactga gaaggccggc tgatcgtggc gggaggggct
     1741 gttcaggctc ccccgcctgt tttttggccc ggcgggggcc cgcgattccc tccagccgtc
     1801 cgttgtgagt ttttcttcct ctcccattga cattcctctc ccctgtccct agaattgatt
     1861 tgtttcttca ataaattaat tatcggtccc cgcgacaggc tgcggggcag aggaggaagc
     1921 ggggatgagg attcgcgcca tcaacgcgga aaaaatgggg ctgtcgaacc ggctgaccct
     1981 gatcaacctc atccggacca accccggcgt gagcaggagg gagctttcca ggctcgccgg
     2041 gcttgatccc agcacggtca ccaagatcat aacctccctt ctggacaagg ggctcgtggt
     2101 tgagcagggc gtccgggacg ccgaccagcc gggcaggaag tccatcatgc tcagggtctc
     2161 cagggatgcg gcggtgtgcc ttgcggcgga ggtcggtgtg gagagcaccc gcatcgctgc
     2221 cggctacctg gacgcatcct ttgaagtgct cgaagagttt tccaccccgc cggaccagga
     2281 ttcttttttc cgggtcttcg ccgaccggat ggagtccatc cggtcctctt cgaagacaag
     2341 ggaattctgc gccctgagtt ttgccgttcc cgggatggtg gagtggggga gcaacgtgct
     2401 gaagcacctt ccccacatgg ggtggaagga tgtcaacctg gccggaaatc tgcggcggac
     2461 ccttccgtgg cttgatattc ccgtgttcgc cgccaacgag gca
//
LOCUS       DAOTHD010000254         2503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_49483_length_2503_cov_2.738562, whole genome shotgun sequence.
ACCESSION   DAOTHD010000254 DAOTHD010000000
VERSION     DAOTHD010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49483_length_2503_cov_2.738562"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1474
                     /gene="mdoH"
                     /locus_tag="P5219_05290"
     CDS             <1..1474
                     /gene="mdoH"
                     /locus_tag="P5219_05290"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HRX26203.1"
                     /translation="GTTMVRMVQSMEARPDIGILQTPPKAVNRETLIARVQQFANHVY
                     GPLLAAGLHFWQLGDAQYCGHNALIRVEPFMKHCELPRLPGKGPLSGDILSHDYVESA
                     LMCRAGYGIWLTYDLKGSWEETPPTLIDELKRDRRWCQGNLQHTRLLFTRGFFPAHRA
                     LFINGILSYGSALLWLIFLTINSAQAISEVLFEPVYMPILRKFLPELPVWRPHWAISL
                     LGSTTLLLFLPKFLGAFPILLADFKARLYGGKIRLFLSILFEVILSMILTPIRMVFHS
                     LFVVSTLLGKSVGWGTQSRDDRGTTWREALRAHWWGSLLGIGWGAVVYWITPSFFWWI
                     SPITFSLALSAPISVFTGRAAVGRLFRKAGLFLIPEEVELPPPLRALEDNLSRPDPFF
                     PFPFSRKKGFLRAVVDPLVHSLHTSLLLRFRRGAPSRERARDRIVEKALACGPDSLTR
                     KERMALLRAPHHLRELHRKVWRLGDEKAAARWGLVPPEKK"
     gene            complement(1549..>2503)
                     /locus_tag="P5219_05295"
     CDS             complement(1549..>2503)
                     /locus_tag="P5219_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26204.1"
                     /translation="TGEHWVTLPLDENGNGIADSWEKAGGMWPCAADSDDDGDPEGKA
                     PGDGLSAYEEYRGFFVKGRHGRLDPNRKDLFVHDPDGLAENAGFGQVTGLAVHYIEPE
                     EGRCTGTDDRARVVNFRSGFSHLVDQHCLWIKKDTLPEPDPFNWGACEGPPEIGPPRT
                     ADRYVLVFADQIRADLERTVRENRPELANALAQRGLRPDAAWFEEQVAAAVATTTLHE
                     ACHGLGISHHYRSLRETLPKGADVEKAIMELDISPSTGQMSCVMRYTRDWGKHPRLTF
                     RAESETLDMLRGRPWPNTLCDTLDDCRGQMVVSDRETGHIF"
BASE COUNT          399 a          895 c          727 g          482 t
ORIGIN      
        1 gggaaccacc atggtccgca tggtgcagag catggaggcc cggccggaca tcggtatcct
       61 ccagacgccc cccaaggcgg tgaacaggga aaccctcatc gcccgggtgc agcagttcgc
      121 gaaccacgtc tacggtcccc tgctggccgc agggctccat ttctggcagc tcggcgacgc
      181 ccagtactgc ggccacaacg cccttatccg ggtggaacct ttcatgaagc actgcgaact
      241 tccccgcctt cccggcaaag gaccgctgag cggcgacatc ctcagccacg actacgttga
      301 atcggctctc atgtgccggg ccggttacgg catctggctc acctacgacc tgaagggaag
      361 ctgggaggaa acgcccccca ccctcatcga cgaactcaag cgggaccgcc ggtggtgcca
      421 ggggaacctc cagcacaccc gcctgctctt caccaggggg tttttcccgg cccacagggc
      481 gctgttcatc aacggcatcc tctcctacgg ctcggccctc ctctggctca tcttcctgac
      541 cataaactcc gcccaggcca tctcggaggt cctcttcgag ccggtctaca tgcccatact
      601 gcgcaaattc ctcccggagc tgcccgtctg gcgcccccac tgggccatat cgctcctcgg
      661 ctccaccaca ctgctcctct tcctgccgaa attcctcggc gccttcccca tcctcctcgc
      721 cgatttcaag gccaggctct acgggggaaa aatacgtctt tttctgagca ttctcttcga
      781 ggtcatcctg tccatgatcc tcactcccat ccggatggtc ttccacagcc tcttcgtggt
      841 ctccaccctc ctgggaaaga gcgtggggtg gggaacccag tcccgggacg accggggaac
      901 cacgtggagg gaagccctcc gggcccactg gtggggctcc cttctgggca taggctgggg
      961 agcggtggtc tactggatca ccccgtcctt cttctggtgg atctcaccca tcaccttttc
     1021 ccttgccctc tccgccccca tctccgtctt caccggacgg gccgccgttg gacggctgtt
     1081 ccggaaggcg gggctcttcc tgatccccga ggaggtggag ctgcctccgc cccttcgggc
     1141 ccttgaggac aacctcagcc ggcccgaccc cttctttccc tttccctttt cccggaagaa
     1201 gggctttctc cgggcggtgg tggatcccct ggtgcacagc ctccacacgt ccctcctcct
     1261 tcgcttccga cggggcgccc cttcccggga gcgggcccgg gacaggatcg tggaaaaggc
     1321 cctggcctgc ggccccgaca gcctgaccag gaaggagaga atggctctcc tcagggcacc
     1381 gcaccatctc agggaacttc acaggaaggt gtggcgcctc ggcgacgaaa aagccgccgc
     1441 ccggtggggc ctggtccctc cagagaagaa atagagaaag cgaggggcag atccttcctc
     1501 gcttcgctcc tcaggatgac aaaaccgaaa aaacacgacc ttcggccgtc agaaaatgtg
     1561 ccccgtctcc ctgtcgctga ccaccatctg cccccggcag tcgtcgaggg tatcgcacag
     1621 ggtgtttggc caggggcggc cccgcagcat gtcgagggtt tcgctctccg ccctgaaggt
     1681 gagccgcggg tgcttgcccc agtcccgggt gtagcgcatc acgcagctca tctgcccggt
     1741 ggatgggctg atgtcaagct ccattatggc cttttccaca tcggcaccct tcggcagggt
     1801 ctccctgagc gaccggtagt ggtgggaaat ccccagcccg tggcaggcct cgtggagcgt
     1861 tgtcgtggcc acggctgcgg cgacctgctc ctcgaaccag gcggcgtcgg ggcggagccc
     1921 ccgctgcgcc agcgcattgg caagctcggg gcggttctcc ctcactgttc tttcgaggtc
     1981 cgccctgatc tggtccgcga aaaccagcac ataccggtcg gccgtccggg gcggcccgat
     2041 ttccggcggt ccctcgcagg ctccccagtt gaagggatcc ggttcgggga gggtgtcctt
     2101 ctttatccag aggcagtgct ggtccaccag gtgggagaag ccggaccgga aattcaccac
     2161 ccgtgccctg tcgtccgtcc cggtgcaccg gccttcctcc ggttcgatgt aatgaaccgc
     2221 cagcccggtg acctgcccga agccggcatt ttccgcaagc ccatcgggat cgtgcacgaa
     2281 gaggtccttt ctgttcgggt cgaggcgtcc gtgcctgccc ttcacgaaaa acccccggta
     2341 ttcctcgtac gctgagagcc cgtccccggg ggcctttcct tccggatcac cgtcgtcgtc
     2401 cgagtcagcg gcgcagggcc acatgccgcc tgccttctcc caggagtcgg cgataccgtt
     2461 cccgttctcg tccaggggaa gggtcaccca gtgctctccg gtc
//
LOCUS       DAOTHD010000255         2501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_49526_length_2501_cov_2.979967, whole genome shotgun sequence.
ACCESSION   DAOTHD010000255 DAOTHD010000000
VERSION     DAOTHD010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49526_length_2501_cov_2.979967"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="P5219_05300"
     CDS             complement(<1..645)
                     /locus_tag="P5219_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRX26205.1"
                     /translation="MNTPDWKTSACPYDCPDGCALRGRFDGNAVTLQPNPTLPYSTFL
                     CEKGMRWASRAVSPSRLSSPLVRKGGNLVPVSWEEALDLWAEKIRDGVERHGPLSVMY
                     LSSAGSLYFSKKLLPHVFAALGGYTAKKGNLCSSAGSLGLKETFGEVPVVRPEEMADH
                     SRGVLFWGRNALETHVHVVPVLKAVRDRGGEAAVLEIRSTPTTRSADRWWRLDPG"
     gene            796..2103
                     /locus_tag="P5219_05305"
     CDS             796..2103
                     /locus_tag="P5219_05305"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRX26206.1"
                     /translation="MSLSKSTVRSLISDSSFPLFLLLVGSFFLTVFFRISASVVLPME
                     GERLGMSAALVGFISSVHFYAYAFLQPVSGILHDRYGPVRVVTSGLLLTAASCLLLTF
                     VRTPFTLGAWRLLSGFGVAPMYSAVLVFQAFAFPPERYCFYAAINLGLSSLGAIVSVA
                     PLGFAVDTFGMTGTFALLSAIPLAMALVLARRAPSDPIRLSAERKETRSFLSIFPGIG
                     QAMLFIMKNRRVRALQTLWAVSSASLLTFQGLWGVSWFAAAFNASSGSARFWSSLVSV
                     GMMLGPVFTSGILVSPAGLPGIIRKVSTANALSWFLLLAAAGWSGAVWMGGLAAFAVG
                     MTSGMRGVFSLAAITAVSPPGMRGSIFGAMNMTAVLSAVVFQWGTGIIIDHFPGEAPG
                     TFTAAGYFAGFTVVAFAMAASLFALHPLGREPLVPDTGEDGKS"
     gene            complement(2114..>2501)
                     /locus_tag="P5219_05310"
     CDS             complement(2114..>2501)
                     /locus_tag="P5219_05310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HRX26207.1"
                     /translation="LPPEEDMYAVIAEEGRHIFLGRKVFFNFNCVILDVCRVTIGDYS
                     MFGPAVQIYTAVHPMEAEARRGREFGRPVTVGSDVWVGGGAILCPGVTVGSRSVIGAG
                     SVVTRDIPEGVFAAGNPCRVIRRVEP"
BASE COUNT          378 a          851 c          759 g          513 t
ORIGIN      
        1 gccgggatca agccgccacc accggtcggc ggagcgggtg gtgggggtgc tccggatttc
       61 gagaaccgcc gcttccccac ccctgtcccg caccgctttc agcaccggga ccacgtggac
      121 atgggtctcc agggcgttcc gtccccagaa cagcaccccc cgggaatggt ccgccatctc
      181 ctcgggccgg acgacgggca cttcgccgaa agtttccttc aggcccaggc ttcccgcgga
      241 cgagcagagg ttcccttttt tcgcggtgta ccctccgagg gccgcaaaga cgtggggcag
      301 cagttttttc gagaaataga gggaaccggc gctggagagg tacatcaccg acagggggcc
      361 gtggcgttcc accccgtccc ggattttttc cgcccagaga tccaaagcct cctcccagga
      421 gacgggaacg aggttccctc ccttccggac aaggggggaa gagaggcggg agggactcac
      481 ggcccgggac gcccaccgca ttcccttctc gcagaggaag gtggaatagg gaagggtggg
      541 gttcggttga agggttacgg cgttcccgtc gaagcgcccg cgcagagcgc atccgtcggg
      601 gcagtcgtag gggcaggcag aggttttcca gtctggggtg ttcatttttc tcctcctcgg
      661 tgcggtctgg atcaatatta ccattccccc ctcccggagg ggacggtcat tttccggtct
      721 ttccgccccc ttctgaactg ccatgacctg tggtatgctg ttcaccacat tgactggagc
      781 aaaggagagt cccccgtgtc attatcaaaa agcaccgttc gcagcctgat ttccgattcg
      841 tctttccccc tctttcttct cctggtgggc tccttcttcc tgaccgtctt cttccgcatc
      901 tccgcatcgg tggtgctgcc catggaagga gagcgcctcg gcatgagcgc ggcgctggtg
      961 ggcttcatct cgagcgtcca cttctacgcc tacgctttcc tccagcccgt ctcgggcata
     1021 ctccacgacc ggtacggacc ggtgagggtg gtgacatcgg gactgctcct cacggcggcg
     1081 tcctgcctgc tgctcacctt cgtgagaaca cccttcaccc tgggggcatg gcggcttctc
     1141 tccggattcg gcgtcgcccc catgtactcc gccgtcctcg tattccaggc cttcgccttc
     1201 ccgccggagc ggtactgttt ttacgcggcc atcaacctgg gactgtcgag cctcggggcc
     1261 atcgtgtccg tggctcccct cggcttcgcc gtggacacct tcggcatgac gggcaccttc
     1321 gccctgctct cggccatccc cctcgccatg gcgctggtcc ttgcccgcag ggccccgagc
     1381 gacccgatcc gcctttccgc cgaaagaaag gaaaccagga gtttcctctc catctttccc
     1441 ggcatcgggc aggcgatgct ctttataatg aaaaaccgcc gcgtccgggc tctccagacc
     1501 ctctgggccg tttcgtccgc ctccctgctc accttccagg gtctgtgggg cgtgtcgtgg
     1561 tttgccgcgg ccttcaatgc ctcgtcgggt tccgcccggt tctggagttc cctggtcagc
     1621 gtggggatga tgctgggccc cgtcttcacc agcggcatac tcgtctcccc cgccggactg
     1681 cctggaatca tacggaaggt cagcacggcc aacgcgctga gctggtttct gctgcttgcg
     1741 gcggcgggct ggagcggtgc cgtgtggatg ggaggcctgg ccgccttcgc cgtggggatg
     1801 acctcgggga tgcggggggt gttctccctg gctgccatca cggccgtctc tccgccgggc
     1861 atgaggggat caatcttcgg cgccatgaac atgaccgccg tgctgtcggc agtggttttc
     1921 cagtggggga ccggcatcat catcgatcat ttcccggggg aagcgcccgg cacgttcacc
     1981 gccgcaggct acttcgccgg gttcaccgtg gtcgccttcg ccatggctgc cagccttttc
     2041 gccctccacc ccctcggaag ggagcctctc gttccggata cgggggagga cgggaaaagc
     2101 tgaccctctc ccgttacggt tcgaccctgc ggatcacccg gcagggattt cccgccgcga
     2161 agacgccttc cgggatgtcc ctggtgacca cgcttccagc gcctatcacg gaccgggaac
     2221 ccacggtcac cccggggcag agaatggctc ccccgcccac ccagacatcg gagcccaccg
     2281 tgacgggcct gccgaactcc ctgccccgcc gggcctcagc ttccatgggg tggaccgccg
     2341 tgtagatctg aactgcgggg ccgaacatgg aatagtcccc gatggtcacc ctgcacacgt
     2401 cgagaatgac gcagttgaag ttgaagaaca ccttccgccc gaggaagata tgcctccctt
     2461 cttctgcgat tacggcgtac atatcttcct cgggcggaag g
//
LOCUS       DAOTHD010000256         2496 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_49642_length_2496_cov_2.909054, whole genome shotgun sequence.
ACCESSION   DAOTHD010000256 DAOTHD010000000
VERSION     DAOTHD010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49642_length_2496_cov_2.909054"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1593
                     /gene="gguA"
                     /locus_tag="P5219_05315"
     CDS             <1..1593
                     /gene="gguA"
                     /locus_tag="P5219_05315"
                     /inference="COORDINATES: protein motif:HMM:NF040905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRX26208.1"
                     /translation="GGALRPLKTPETGADLMAKELLLMRNITKTFPGVRALKDVCLDV
                     EEGEIHAIVGENGAGKSTLMNILSGVYPSGEYEGEILFDGEPCVFRSIRDSEDKGIVI
                     IHQELALVPYLSIAENVFLGNERARRGVVDWDETYTRTAGLLEKVHLNEKPSVLTKDL
                     GVGKQQLVEIAKALSKHVRLLILDEPTAALNDEDSGRLLTLLLDLKAEGITSIIISHK
                     LNEVEKIADRITVLRDGAVIETLDNGERTIREDRIIKGMVGREIVDRFPKRDTRPSDE
                     IALAVENWTVRDPLRPDCLKIKNVSFTVRRGEVVGLVGLMGAGRTELAMSIFGRSYGM
                     DISGRILLGGEPAELPSPKEAIGRGLAYLTEDRKSSGLILIDDICRNISLASLSRLCG
                     RKGVIDCNREVTAAEGLRKRLGIKTPSIFQKTGNLSGGNQQKTVLARWILSDPDVLLL
                     DEPTRGIDVGAKYEIYCIINELAAAGKAVVLISSEMPEVLGMSDRIYVMNEGRIVGEL
                     SREEASQEAIMKCIMQSNREVA"
     gene            1593..>2496
                     /gene="gguB"
                     /locus_tag="P5219_05320"
     CDS             1593..>2496
                     /gene="gguB"
                     /locus_tag="P5219_05320"
                     /inference="COORDINATES: protein motif:HMM:NF040906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRX26209.1"
                     /translation="MEKMKEMFMANIRQYAMMVALIFIVILFQYLTEGVLLRPLNITN
                     IIQQNSYILILAIGMLLCILTGNIDLSVGSVAAFIGAVAATMMVTWKWPVLPSVLLAL
                     LLGVAIGCWQGYWIAYFRIPAFIVTLAGMLCFRGLTLVMLRGQTIAPMPQSFQKSIST
                     FIPDVLGGADLNYTALAVVAAAILAFAFADLRSRRKKIACGFDVLSFPLWTLKILTMG
                     AVLMAFSWQLARHRGLPVVLVLLGSLVLIYSFITGKTVFGRHVYAFGGNEKAAKLSGI
                     DTNRVFFLVYANMGLLAAVAGLVFM"
BASE COUNT          484 a          763 c          784 g          465 t
ORIGIN      
        1 ggcggagcgc tccgccccct gaaaacaccg gagacggggg ctgaccttat ggcgaaagaa
       61 ctcctgctga tgcggaacat caccaagacc ttccccggcg tgcgcgccct gaaggatgtc
      121 tgtctcgacg tggaggaggg cgagattcac gccatcgtcg gcgagaacgg cgccggcaaa
      181 tcgacactga tgaacatcct ctccggggtg tacccttccg gagaatacga aggggagatt
      241 ctcttcgacg gcgaaccctg cgtcttccgg tccatccggg acagcgagga caaggggatc
      301 gtcatcatcc accaggaact cgccctggtg ccctaccttt ccattgcgga aaacgtcttt
      361 ctcggcaacg agcgggcccg acgtggcgtg gtggactggg acgagaccta cacacgcacc
      421 gccgggcttc ttgaaaaggt tcacctgaac gaaaagccgt cggtcctgac gaaggacctc
      481 ggggtgggca agcagcagct cgtggaaata gccaaggcac tctccaagca cgtccggctg
      541 ctcatcctgg acgaacccac ggccgccctg aacgacgagg acagcgggcg ccttctcacg
      601 ctgctgctgg acctcaaggc cgagggtatc acctccatca tcatatccca caagctgaac
      661 gaagttgaaa agatcgccga ccggattacc gtgctccggg acggcgccgt catagagacc
      721 ctggacaacg gggagcggac catccgggag gaccggatca tcaagggaat ggtgggccgg
      781 gagatcgtgg accggttccc caaaagggac acccgcccgt cggacgagat tgccctggcg
      841 gtggagaact ggacggtccg ggacccgctc cgccccgact gcctgaagat caagaacgta
      901 agcttcaccg tgcggcgggg agaggtggtg ggcctcgtgg gcctcatggg cgccgggcgg
      961 acggaactgg ccatgagcat cttcggccgc tcctacggca tggacatctc gggacggatc
     1021 ctcctgggag gagagcctgc ggaactgccc tcgccgaagg aggccatcgg ccgcggcctg
     1081 gcatatctca ccgaggaccg gaaatcctca ggactgatcc tgatcgacga catctgccgc
     1141 aacatctccc tggcgagcct gtcacgactc tgcggacgga agggcgtcat cgactgcaac
     1201 cgcgaggtga cggcggctga gggactacgg aagcggctcg ggatcaagac gcccagcatc
     1261 ttccagaaga cggggaacct gtccggagga aaccagcaga agacggtgct cgcccggtgg
     1321 attctgtccg atcccgacgt gctgctcctc gacgagccca cccggggcat cgacgtgggg
     1381 gcgaaatacg agatttactg catcatcaac gaactggccg cggcgggcaa ggccgtggtg
     1441 ctcatctcct ccgaaatgcc ggaggtgctc ggaatgagcg accggattta cgtgatgaac
     1501 gagggacgga tcgtgggcga gctgtcccgg gaagaggcct cccaggaggc catcatgaag
     1561 tgcatcatgc agagcaatcg ggaggtggcc tgatggagaa gatgaaagag atgttcatgg
     1621 cgaacatccg ccagtacgcc atgatggtgg cgctcatctt catcgttatt cttttccagt
     1681 acctgactga gggcgtgctg ctccggcccc tgaacatcac caacatcatc cagcagaaca
     1741 gctatattct catcctcgcc atcggcatgc tcctgtgcat cctcacgggg aacatcgacc
     1801 tctcggtggg ttccgtggcg gcctttatcg gcgccgtggc cgccaccatg atggtcacct
     1861 ggaaatggcc tgtgctgccc tcggtgcttc tggccctgct cctcggtgtg gctatagggt
     1921 gctggcaggg gtactggatc gcctacttcc gcatccctgc cttcatcgtc accctggccg
     1981 gaatgctctg tttccgtggg ctgacgctgg tcatgctgag gggacagacc atcgcgccca
     2041 tgccccagtc gttccagaag tccatttcca ccttcatccc cgatgttctg gggggagcgg
     2101 acctgaacta cacggctctc gcggtggtgg cggctgccat cctcgccttc gccttcgccg
     2161 acctgaggag caggcggaaa aaaatcgcct gcggcttcga cgtgctttct ttccccttgt
     2221 ggacgctgaa gattctcacc atgggagcgg ttctcatggc gttttcctgg cagctcgccc
     2281 gtcaccgggg tcttccggtg gtgctggtgc tcctgggctc cctggtgctg atctattcct
     2341 tcatcacggg aaaaacggtc ttcggacggc acgtctatgc cttcggcggg aacgaaaagg
     2401 cggcgaagct ctccggcatc gacacgaaca gggtgttttt cctcgtctat gccaacatgg
     2461 gactcctggc ggcggtggcg gggctggtct ttatgg
//
LOCUS       DAOTHD010000257         2495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_49674_length_2495_cov_2.275410, whole genome shotgun sequence.
ACCESSION   DAOTHD010000257 DAOTHD010000000
VERSION     DAOTHD010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49674_length_2495_cov_2.275410"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..1540)
                     /locus_tag="P5219_05325"
     CDS             complement(146..1540)
                     /locus_tag="P5219_05325"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HRX26210.1"
                     /translation="MTKNGKHENSSPAAGTPERKLLCRGLAASPGLAAGKVVLVPGSA
                     DAVKITDGDILVTTMTTPDMIPALRKAGAVVTDEGGRTCHAAILSRELGIPCIVGARN
                     ATAVLAEGQDVIVDATRGVVFQGEEKTAPSHETVVSSGPAAAPRQPAQVLPPTAPQSV
                     TATKILLNLEGPASVERCSALPADGVGLLRTEFIFTGDIGIHPIYLVRTKQETLFVDK
                     LAEGIAAVASGFSPRPVLMRFSDFRTNDFRKLRGGQDIEPLESNPMLGWRGASRYVSS
                     DYEQGFRLECRAVKKVRDTFGLTNLSVIIPFVRTLREMEQVRGILESEGLVSGPSFKV
                     WLMVEVPSVVLQADRFAAICDGFTIGSNDLTQLILGVDRDSAKLDAMGYFDERHPSVL
                     EAISMAVRAARKAGIPCSICGEGPSVFPDFAEKLVAMGIDSISVNPDALDYTRRIVAS
                     AEQKIILDGIRGRV"
     gene            complement(1607..>2495)
                     /locus_tag="P5219_05330"
     CDS             complement(1607..>2495)
                     /locus_tag="P5219_05330"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRX26211.1"
                     /translation="SANEAERRKALDELSGFVKNDMKATLEAMDGLAVTFRLLDPPLH
                     EFVPQSAEKQAELAKALGIEPADVAKRGESLHESNPMMGHRGVRLGITYPEITEMQVR
                     AMFEATAELLKEKKNPKPELMVPVTCDVSELDVTRKVVDRVYAEVLKKTGLDKIEFKY
                     GTMIEIPRATLLADRMAKSAEFFSFGTNDLTQMTFGYSRDDSGAFLPEYINQNILPQD
                     PTASLDQEGVGQLVDWAAKLGRGVKKELKLGICGEHGGNPSSIAFCHAAGLNYVSCSP
                     FRVPVARISAAHAAMGKIS"
BASE COUNT          456 a          695 c          807 g          537 t
ORIGIN      
        1 ccctccgggg acgcgatcgc ttcgctcagg atgacagcaa aaacgagatc cctcggtcgc
       61 ttcgctcgct cgggatgaca aacaagggca aaagcgagac tcctcgtcgc aaaagactgc
      121 tccccggaat gactcttctt cccccctaca ccctgccccg tatgccgtca aggataattt
      181 tctgctcggc cgaggcgacg atgcgccgtg tgtagtccag ggcgtcgggg ttgacgctga
      241 tgctgtcaat tcccatggcg acgagctttt cggcgaagtc cgggaagacg gaaggtcctt
      301 cgccgcagat ggagcacggt atgcccgctt tccgggccgc ccggacggcc atggatatgg
      361 cctccaggac ggaggggtga cgctcgtcga agtagcccat ggcgtcgagc ttggctgagt
      421 cgcgatccac gccgagaatg agctgggtga ggtcgttgct tccgatggtg aacccgtcgc
      481 agatggcggc gaaccggtcg gcctgcagca cgaccgacgg gacttccacc atgagccaga
      541 ccttgaagga cgggccggag acgagccctt cggattcgag gattcccctg acctgctcca
      601 tttccctcag ggttcggacg aaggggatga tgaccgagag gttggtgagg ccgaaggtat
      661 ccctaacttt cttcacggcg cggcattcga gacggaagcc ctgttcgtaa tcggaggaga
      721 cgtatcggga cgctcccctc cagccgagca tggggttgga ttcaaggggt tcgatatcct
      781 gtccgccccg gagcttgcgg aagtcgttgg tccggaagtc gctgaagcgc atgagcacgg
      841 gcctgggaga gaatcccgag gcgacggcgg cgatgccttc ggcgagcttg tccacgaaga
      901 gggtctcctg tttggtgcgg acaaggtaga tgggatggat gccgatgtcg ccggtgaaaa
      961 taaattctgt cctgaggagg ccgacaccgt cggcgggcag ggcagagcac cgctccaccg
     1021 atgccgggcc ttcaaggttg agaagtatct tggtggcggt gacgctctgg ggagccgtcg
     1081 gcggaaggac ctgcgcgggc tgtcggggtg cggcggcagg tccgctggag acgacggttt
     1141 catgggatgg agccgtcttt tcctctccct ggaacaccac tcccctggtg gcgtccacta
     1201 tcacgtcctg tccttcggcg aggaccgccg tggcgttccg ggcgcccacg atgcagggta
     1261 ttcccagctc ccgggagagt atggcggcgt ggcatgtgcg gcccccttcg tcggtgacga
     1321 cggcgccggc ctttcggagc gcggggatca tgtccggcgt ggtcatggtg gtcacgagga
     1381 tgtcgccgtc ggtgatcttc accgcgtcgg ctgatccggg tacaaggacg acctttccgg
     1441 cggcgaggcc gggagaggct gcgaggcccc tgcagaggag cttccgttcg ggagttccag
     1501 cggccgggga ggagttctcg tgcttcccgt tctttgtcat gatctcaggt cctttcagaa
     1561 agttccgcgc cggggggtgc cccggcgcgg tggctctttt tctgttctag cttatcttgc
     1621 ccatggccgc gtgagcggcg gaaatccggg cgacaggcac tctgaagggc gagcagctca
     1681 catagttaag gcccgccgca tggcagaagg cgatgctgga ggggttgcct ccgtgctcgc
     1741 cgcagattcc cagtttgagc tccttcttga ctccccggcc cagtttggcc gcccagtcca
     1801 ccagctgtcc cacaccttcc tgatcaaggg aggcggtagg atcctgggga aggatattct
     1861 gattgatgta ctccggcagg aaggcgcccg aatcgtcccg ggaatagccg aaggtcatct
     1921 gggtaaggtc gttggtaccg aacgagaaga actcagcgct tttagccata cgatcggcca
     1981 ggagtgtggc acgtggaatc tcaatcatgg tgccgtattt gaattcgatc ttatcgagac
     2041 cggttttctt aagaacctcg gcatatactc tgtcgactac ttttcttgta acatcaagtt
     2101 cagaaacatc gcaggtaacg ggaaccataa gttcgggttt gggatttttc ttctccttga
     2161 ggagttcggc agttgcttcg aacatggcac ggacctgcat ctcggtaatc tcagggtagg
     2221 tgattccgag acggacacca cggtgaccca tcatcgggtt gctttcgtgc agtgattcgc
     2281 cacgttttgc aacatcagcc ggttcaattc caagggcttt ggcaagttct gcctgtttct
     2341 ctgcgctctg gggtacgaac tcatgaagag ggggatcaag aagcctgaag gtaactgcaa
     2401 ggccatccat tgcttcgaga gtggctttca tatcgttttt aacaaagccg gagagctcgt
     2461 cgagggcttt tctgcgttcg gcctcatttg cactg
//
LOCUS       DAOTHD010000258         2493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_49720_length_2493_cov_3.527482, whole genome shotgun sequence.
ACCESSION   DAOTHD010000258 DAOTHD010000000
VERSION     DAOTHD010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49720_length_2493_cov_3.527482"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1690
                     /locus_tag="P5219_05335"
     CDS             <1..1690
                     /locus_tag="P5219_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26212.1"
                     /translation="MGDIQGFTFSGFGPGDILGAGESSAPDGNPDAAFSVSMTGIGAL
                     ANFSLRSEDGKSTWDTTARNNVAGIHVQDSAGKLLTDSAGSMPITPFLLGASFVLTVH
                     DDGSLARGGKFVLTALFVDGSESSATVEIPPTVKEEPEQAAQVKILSARWSDEGKRDL
                     TGDYEKLAGNGVSDEAIRVVVQGSGKLTGVTVRSIKGDAAEWDTLPGNSAWLVGVTSA
                     EKVLNRKDGSIEADLKGRTTLDLRLTDNGSIKRGRSTFEVSLTFSDGSVVKRTVAAKQ
                     GGPSGDAFDGSAAFLGSGNRDLTGRNEKRAGNGKPDLQAELRVETTGTIVAAKIHTIS
                     GASGEWDTVPGNGKWLLVLTGADSAILNRPDGSISLAVSGPSVLHLWFEDNGAFSERE
                     TRAVVTLTYDDGRVLSRQLQFPSSRGPRKEPENDVQKEEQREIRLSPPRQASSSNYVG
                     KGERPGKSGNRDWVFELQITGKGTIESVSLQGHSSTGMAIWDTMPGNGFPLLGITGKG
                     KGLLNKADGTVSFDVPPHNNLMLFVEDDGFLRRRGQKQFKVTVAWSDGTVTETN"
     gene            1758..2351
                     /locus_tag="P5219_05340"
     CDS             1758..2351
                     /locus_tag="P5219_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26213.1"
                     /translation="MRRFTFPPPALVFLFLLFFACPSHAYSRYPLGGVWEYSGSVTVT
                     IDGQQAVLRDSGRITIDSDYSYDWWSSDFDEYIEAFRARGSFSVSLTPEIREDYHHSD
                     WVNHWYNFRSFEITIDNVQYYMELTGRTRADLRITRTISGRRVSAVFSASRIRWDNDW
                     DDDYDDGWHYGIGAGCDIGTPLSLLFLLPLLFLCRKK"
BASE COUNT          591 a          676 c          744 g          482 t
ORIGIN      
        1 catgggtgat atccagggtt tcaccttttc cggcttcggc ccgggtgaca tcctcggtgc
       61 aggagagagt tcagctcccg acggcaaccc ggacgcagcc ttttccgtat ccatgacggg
      121 catcggcgcg ctggccaact tttcgctccg ttccgaagac gggaaatcaa catgggatac
      181 aacagcaaga aacaacgttg ccggcatcca cgtacaggac agcgcgggaa agctcctcac
      241 ggacagcgcc ggaagcatgc ccataactcc attcctcctt ggggcatcct tcgtgctcac
      301 ggtgcatgat gacggctccc tggccagggg gggcaaattc gtcctgacgg cccttttcgt
      361 cgacgggtcg gaaagttcgg ccacggtgga aattccgcca acggtgaaag aagaacccga
      421 gcaggcggcc caggtcaaaa ttctttccgc cagatggagt gacgagggga aacgggacct
      481 cacaggcgat tacgaaaaac ttgcagggaa cggcgtgtct gacgaagcaa tccgcgtagt
      541 ggtccagggc agcggaaagc tgactggcgt aacggtacgg agcatcaagg gcgacgcagc
      601 ggaatgggac actctccccg gcaacagcgc ctggctcgtg ggagtcacat ccgccgaaaa
      661 agtgctgaac cgcaaggacg gaagcatcga agcagacctg aagggaagga ccaccctcga
      721 ccttcgactg actgataacg gttctatcaa aagggggagg tcaaccttcg aagtgagcct
      781 cactttctca gacggctcag tggtgaagcg cacagtggcc gcaaaacagg gcggaccttc
      841 cggagacgca ttcgatggga gcgcggcgtt tctgggctcg ggaaaccgtg acctcaccgg
      901 aagaaatgaa aagagggcag gcaacggcaa gccggatctc caggcggagc tcagggtgga
      961 aacgacggga accatcgtgg cggcaaaaat acataccatt tccggagcct ccggtgaatg
     1021 ggacaccgtc cccggaaacg gcaaatggct tctggtgctg acgggagccg acagcgccat
     1081 tctcaacagg cccgacggat ccatatccct cgcggtttcc ggaccttcgg tccttcatct
     1141 ctggttcgag gacaacggcg ccttttctga aagggaaacc agggccgtgg tgacgttaac
     1201 atacgatgac ggccgcgttc tgtcccggca gcttcagttc ccttcgtcca gggggccccg
     1261 gaaggagcct gaaaacgacg ttcagaagga agaacagcgg gagatcaggc tctctcctcc
     1321 cagacaggct tcaagcagca attacgtcgg caagggggag cggccaggaa aaagcgggaa
     1381 tagagactgg gttttcgagc tccagatcac cgggaaagga acgatcgaaa gcgtctctct
     1441 ccagggccat tcttcaacag ggatggccat ctgggatact atgcccggaa acggcttccc
     1501 ccttcttgga atcacgggaa aagggaaagg tcttctgaac aaggcggacg gaacggtttc
     1561 cttcgatgtt cctccccaca acaacctcat gctctttgtg gaagacgacg gtttcctcag
     1621 gaggagaggc cagaagcagt tcaaagtgac ggtggcatgg agcgacggaa ctgtcacaga
     1681 aaccaactga gaaacagatt tttccgggga tggagaattt ctccatcccc ggaacttcaa
     1741 aaagggggct gatgccgatg agacgtttca cattcccgcc gcctgccctg gtatttctct
     1801 ttcttttgtt ttttgcctgc ccatctcatg cctacagccg gtatcccctg gggggggtat
     1861 gggaatattc cggaagcgtc acggtgacca ttgacggaca acaggcagtt ctcagggaca
     1921 gcggaagaat aaccatcgat tccgattatt cctacgactg gtggagcagc gatttcgacg
     1981 aatatatcga ggcctttcgg gccagggggt ccttttcggt ttcactgact ccggaaatcc
     2041 gggaagacta tcatcactcg gattgggtca atcattggta taacttccgg tccttcgaaa
     2101 ttaccattga caatgtacag tattacatgg agctgacagg ccgaacgagg gcggacctga
     2161 gaattacccg gacaatcagc ggccggcggg tatcggcggt tttttccgcc tcccggatca
     2221 ggtgggacaa cgattgggac gatgattacg acgatggatg gcattacggt atcggggcag
     2281 ggtgcgatat aggaacgccc ctatcccttc tgtttctgct gccgctgctt ttcctctgcc
     2341 ggaaaaaatg atggagagcc tgcttcgttc cccgctggac atccacaaga gagtatggta
     2401 aaaattacca gtgccggccg gggtggcgga atggtagacg cagcggactc aaaatccgcc
     2461 gtgggcgact acgtgggggt tcgagtcccc ccc
//
LOCUS       DAOTHD010000259         2488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_49864_length_2488_cov_3.932594, whole genome shotgun sequence.
ACCESSION   DAOTHD010000259 DAOTHD010000000
VERSION     DAOTHD010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49864_length_2488_cov_3.932594"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="P5219_05345"
     CDS             complement(<1..588)
                     /locus_tag="P5219_05345"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HRX26214.1"
                     /translation="MKLLIINPNSNDEFTGLIRECAEGAASPGTDITCVSTPGAPPLI
                     DNYIDEIACGPGMVKIMRENEASFDAFIVACTCDVNIDILREMTEKPVAGIGESSMMA
                     AMMLGGKFSVIQMGPRGIPMKERFVRKLGFESRCASVRSIDPAGPGDMGDRVIAAARK
                     AVEEDGAEVLTLGCAGLAGLDERTSRAVGVPVIDGV"
     gene            complement(585..1958)
                     /gene="allB"
                     /locus_tag="P5219_05350"
     CDS             complement(585..1958)
                     /gene="allB"
                     /locus_tag="P5219_05350"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR03178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HRX26215.1"
                     /translation="MFDLVIENGTVVNSRGAFEGKVAVKNGRIAALLAGDVPAEAKEV
                     LDARGLMVLPGLVDAHVHCGHGTPEREDFACVSRAAAAGGTTLMVDMPLSEPTTVTVE
                     ALEGKIASAGKQSVVDFALYSGIIPGHLDDIESTFSAGAQTYKIFTCRCSNYPMTYDG
                     ALLKGMRTVGKLGGMVCAHAENDTLIQELVDDLQAAGRNDARAFLDSHPEYTELEAVN
                     RFIFLAGLVPECRAHICHLSIADGVRAVREARGRGMNNLSIETCPQYLALDEDDLVEG
                     GAFAKCDPPVRRRETVEELWKYVLDGTVDMIASDHSPHSFEKKTAEGGNFWTVSEGCT
                     GVQTLLPVVLTEGRKRGLSWKRLVELCCTRPAELFGLSGRKGDIAPGLDADFALVDPG
                     AEWVLKDEDLFYRNRWSPYTGKTFRGKVVKTVVRGRTVFDGGKICAEEGYGTFCPMDM
                     KGRNGQP"
     gene            complement(2020..2463)
                     /locus_tag="P5219_05355"
     CDS             complement(2020..2463)
                     /locus_tag="P5219_05355"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRX26216.1"
                     /translation="MSSLFFSRFFALTDIPTELAMLVQGWDVPRWVILFGILVVYFLL
                     GMIMVEIGIYALTLPIFMPIIISLGYDPIWFGVVVLKLSEIAAITPPVGLNVYMAKAV
                     AGKNVSLEEIFRGIWPFCLCDIIVLIVLILFPQLSLWLPDLLMGN"
BASE COUNT          488 a          802 c          699 g          499 t
ORIGIN      
        1 cacgccgtcg atcaccggga ccccgacggc ccgggaggtt cgttcatcca gcccggcaag
       61 cccggcacat cccagggtga gaacttcggc tccgtcctct tccaccgctt tccgggcggc
      121 ggcgatgacc ctgtcgccca tgtccccggg gccggcggga tcgatggagc ggaccgaagc
      181 gcaccgggac tcgaagccga gcttcctgac aaaccgctcc ttcatgggaa tgcccctcgg
      241 ccccatctgg atgaccgaga acttcccgcc gagcatcatg gccgccatca tggaggattc
      301 cccgataccc gccaccggct tttccgtcat ctcccggagg atgtcgatgt tcacgtcgca
      361 ggtgcaggcc acgatgaagg cgtcgaagga tgcttcgttc tccctcatga tcttcaccat
      421 gccggggccg caggctatct cgtcgatgta gttgtcgatc agcggaggcg cccccggcgt
      481 gctcacgcag gtgatgtcag tgcccgggga tgcagcacct tcggcgcatt cccggatcag
      541 ccccgtgaac tcatcgttcg aattggggtt tatgatcagc agtttcacgg ctgcccgttc
      601 ctccctttca tgtccatggg gcagaaggtg ccgtacccct cttcggcgca gatttttccg
      661 ccgtcgaaaa ccgttctgcc cctgacgacg gtcttcacca ctttccccct gaaggtcttc
      721 cctgtatagg gactccaccg gttgcggtaa aaaagatcct cgtccttcag cacccattcg
      781 gcgccggggt ccacaagggc gaaatctgcg tccaggcccg gggcgatgtc ccctttccgg
      841 cctgaaagtc cgaaaagctc cgcgggacgg gtgcagcaca gttccacgag cctcttccac
      901 gaaagccccc gcttcctgcc ctccgtcagc actacgggaa gaagcgtctg gactcccgtg
      961 catccctcgg ataccgtcca gaaatttccc ccctcggccg tctttttctc gaaggaatgg
     1021 ggagagtggt ccgaggcgat catgtccacc gtaccgtcca gcacgtattt ccagagttcc
     1081 tcgaccgtct cccgtcttct cacgggaggg tcgcatttcg cgaaggcccc tccttcgacc
     1141 aggtcgtcct cgtcgagggc gaggtactgg gggcacgtct cgatggagag attgttcatc
     1201 cccctgcccc tggcctcacg gactgccctc accccgtcag cgatgctgag gtggcagatg
     1261 tgggcccggc attcgggaac gaggcccgcg agaaaaatga acctgttgac cgcctccagt
     1321 tcggtatatt ccgggtggga atccaggaat gccctcgcgt cgtttcttcc cgccgcctgg
     1381 agatcgtcca ccagttcctg gatgagagtg tcgttctcgg cgtgggcgca gaccatcccc
     1441 ccgagttttc ccaccgttct catccccttc agcagggcgc cgtcataggt catggggtag
     1501 ttcgagcacc tgcaggtgaa aatcttgtag gtctgggcac ctgcggaaaa ggtggactcg
     1561 atgtcatcca ggtgaccggg gataatcccg ctgtagaggg caaaatccac cacggactgt
     1621 tttcccgctg atgctatctt gccctccaga gcctccaccg tcacggtggt gggctccgaa
     1681 aggggcatgt ccaccatgag ggtcgttccc ccggcggcgg cggccctgct gacgcaggcg
     1741 aaatcctccc gttcgggagt gccgtggccg cagtgtacgt gggcgtccac gagtccgggc
     1801 aggaccatca gcccccgggc gtcaagaact tccttcgctt cagcgggaac atcaccggcg
     1861 agcagcgcgg cgattctgcc gtttttcacg gcgacttttc cctcgaatgc tccccgggaa
     1921 ttcactacgg taccgttctc gatcaccagg tcaaacacgg gcatcacctc ggataattct
     1981 ggtcattgta tctgtccggc ttcacgcccg gcagcaccgc tagttcccca tgagaaggtc
     2041 cggaagccag agggacagct gggggaagag aataagcacg ataaggacta ttatgtcaca
     2101 aaggcagaag ggccagatgc ccctgaaaat ctcctcgagg gaaacatttt ttccggcgac
     2161 ggccttggcc atgtacacgt tcagccccac cggaggcgtt atcgccgcga tttcactcag
     2221 tttcaggacc acaacaccga accagatcgg atcatacccc aaagagatga tgatgggcat
     2281 gaaaatcggc agggtgaggg cgtagattcc gatctccacc atgatcatgc cgaggaggaa
     2341 ataaaccacg aggatgccga aaagaatgac ccacctcgga acatcccatc cctggacaag
     2401 catcgccagc tccgtcggaa tgtccgtcag cgcgaaaaac cgggaaaaga aaagacttga
     2461 gatttttgca taactatgcc cactttac
//
LOCUS       DAOTHD010000260         2482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_50034_length_2482_cov_2.920478, whole genome shotgun sequence.
ACCESSION   DAOTHD010000260 DAOTHD010000000
VERSION     DAOTHD010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50034_length_2482_cov_2.920478"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2482)
                     /locus_tag="P5219_05360"
     CDS             complement(<1..>2482)
                     /locus_tag="P5219_05360"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRX26217.1"
                     /translation="ESNSFVVLEMEVDVNVDQALVDVSNKVKSARNKLPDSVDEPIFR
                     KVDINAQPFLVVAFTSSLPEKEAKKVIEDNIVPRLSRVDGVGQTSVTGGRDREVRILL
                     DPVALNEYRVSIQTLAAVVAANNVTNPSGYITQEKDETWLRLIGEFNRVEELENIMIP
                     TASGNPVRVRDLGTVVDGEQDVRSIARVNGNSVVQLRISARPNSDVVRAGELVKKELD
                     KILKTMPDFTAEIPFDDTKFIESSVKNVLRDMGVGILLTVVVLYLFLKRFSATFIVSV
                     AMPVALFATFMPLAMHGYSLNIMSSLGLAISMGVLVNNSILIIENIYRFRDMGYSPEE
                     AAEKGTVEISLSVIAGTATNLGVFLPVAIMSGIAGQFLVQYAMTIVYATLFSLWVSLT
                     LTPSMAARFKPDKGIPRIGRILCGWWDWIYSGFEELFLFFLRRALAHPLITIFLFLGL
                     TFGAFRLGGLIGSEMMPRTDSGVMTVSITLPSNTPVFVTRNRAKEIEDHIRKTLPSLK
                     YVVSTVGASGRNQGTHKATIEAYFEEDPGRPSTMEMADRIRPFVNSMPGVDGSVSGFR
                     QNMGGGKPIQLVVRGEELETLYRIAETIRDRIRHVPGIVDAGIDVEMGKPELQILPVR
                     WRLSQFGIDMTTLSHVIYGYLVGMDAGKFRQDGFEYDIKARISPDKAKDIFKVQDLPI
                     MTGYGLMPLKEFAEVQWRDGPTEVVRKDRQKAVTVEADARYISVGEATANARRAIADL
                     DLPEGYSIDYGGDAEFMEETFGELGQALFIAIGLTFIIIAAILESWFFAFVIILTVPL
                     SALGVIPSMLLSGVSVSMFALIGLIMLVG"
BASE COUNT          482 a          748 c          764 g          488 t
ORIGIN      
        1 tcccaccagc atgatgaggc cgatgagggc gaacatggag acggaaaccc ccgagaggag
       61 catggacgga atgactccaa gggcggacag gggaaccgtg agaatgatca cgaaagcgaa
      121 gaaccacgat tcgaggatgg ccgcgatgat gatgaatgtg agcccgatgg cgatgaagag
      181 cgcctggccg agttccccga aggtctcctc catgaattcg gcgtctcccc cgtagtcgat
      241 ggagtatccc tcgggaaggt cgaggtccgc tatggcccgg cgggcgttgg ccgtggcttc
      301 gccgacgctg atgtaacggg cgtccgcttc gacggtgacc gccttctgcc tgtctttccg
      361 gacgacctcg gtgggaccgt ccctccactg cacctcggcg aactccttca gcggcatgag
      421 accgtagcct gtcatgatag gcaggtcctg gaccttgaag atatccttcg ccttgtcagg
      481 gctgatccgg gccttgatat cgtactcgaa gccgtcctgg cggaacttcc cggcatccat
      541 gccgacaaga tagccgtaaa tcacgtggga cagggtggtc atgtcgatgc cgaactggga
      601 gagcctccac cgcaccggca gaatctgcag ttccggtttg cccatttcaa cgtcgatgcc
      661 tgcgtccacg atgcccggca cgtggcggat gcggtcccgt atggtctccg cgatccggta
      721 gagggtttcg agctcctctc ccctgaccac gagctggatg ggctttccgc ctcccatgtt
      781 ctgccggaac ccgctgaccg aaccgtcaac gccgggcatg gagttcacga agggacggat
      841 gcgatccgcc atctccatgg tggagggccg gccgggatcc tcttcaaagt aggcttcgat
      901 ggtcgccttg tgggttccct gattccttcc cgaggcgccc acggtggaga cgacatattt
      961 cagcgaggga agggtcttcc tgatgtgatc ctcgatttcc ttcgccctgt tccgggtcac
     1021 gaacaccggg gtgttgctcg ggagggtgat gctgacggtc ataacgccgc tgtcggtgcg
     1081 gggcatcatt tcggatccga taagccctcc cagcctgaag gccccgaagg tgagccccag
     1141 gaaaagaaat atggtgatga gcggatgggc aagagcccgc ctgagaaaga agaggaacaa
     1201 ctcctcgaag ccggagtaaa tccagtccca ccacccgcag agaatgcgcc cgatcctcgg
     1261 aatccccttg tcgggcttga accgcgcggc catggacggc gtgagggtca gggagaccca
     1321 cagggagaaa agagtggcgt acacgatggt catggcgtac tgcaccagga attggcccgc
     1381 gatcccgctc ataatggcca ccggcaggaa tacaccgagg ttcgttgccg ttcccgcgat
     1441 gaccgacagg gagatctcca ccgttccctt ttccgccgcc tcctcggggg agtaccccat
     1501 gtcccgaaag cggtagatgt tctcgatgat gaggatggag ttgttcacca gaacgcccat
     1561 ggagatggca agccccaggg agctcatgat gttcagcgag tagccgtgca tggccagggg
     1621 catgaaggtg gcgaaaagcg ccaccggcat ggccaccgag acgatgaacg tggccgagaa
     1681 ccgcttgagg aagaggtaga gcaccaccac ggtgagcagg atgccgaccc ccatgtcccg
     1741 gagtacgttc ttcaccgacg actcgatgaa cttcgtgtcg tcgaagggta tctcagccgt
     1801 gaagtcgggc atggtcttca aaatcttgtc gagttccttc ttcacgagtt cccctgccct
     1861 cacgacgtcg gaattggggc gggcgctgat ccggagctgc accaccgagt ttccgttgac
     1921 ccgggcgatg gagcggacat cctgctcgcc gtccaccacc gttcccaggt cccgtacccg
     1981 caccgggttg cccgatgccg tggggatcat gatgttctcc agttcttcga cccggttgaa
     2041 ctcgccgata aggcggagcc aggtctcgtc cttctcctgg gtgatgtagc ccgaaggatt
     2101 ggtgacgttg tttgccgcca ccaccgccgc aagggtctgg atgctcaccc ggtattcatt
     2161 cagggccacg gggtccagca atatccgaac ctccctgtcc cggcccccgg tcacgctggt
     2221 ctggccgacc ccgtccaccc tggagagacg gggaacgatg ttgtcctcga taaccttctt
     2281 cgcttccttc tcgggaaggg agcttgtgaa ggcgacaaca aggaagggct gggcgttgat
     2341 gtccaccttc ctgaagatgg gttcgtccac gctgtcggga agcttgttcc tggccgattt
     2401 caccttgttc gacacgtcca cgagggcctg gtcgacgttt acgtccacct ccatctcgag
     2461 gaccacgaag gagttgctct cc
//
LOCUS       DAOTHD010000261         2480 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_50090_length_2480_cov_3.531134, whole genome shotgun sequence.
ACCESSION   DAOTHD010000261 DAOTHD010000000
VERSION     DAOTHD010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50090_length_2480_cov_3.531134"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="P5219_05365"
     CDS             <1..782
                     /locus_tag="P5219_05365"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRX26218.1"
                     /translation="LELANPLSAEQSKMRTTLLPGLLRSVELSVQAGWKAPVRIFELG
                     RVFLPLDEGGHEEVERISGLVYGGRDPRLPFGPAGADDLFSLKGDILALAESRGVSLD
                     FEQGEEPFGHKGQTAVIMNAGRAAGYLLRLKPAIEKDIECSPVYAFELDLELLVKESL
                     PLFRENSPFPPVYRDISLIVPVDSPMEEVSAAIRSEGGELLRSIRLFDIYSGKGIPDG
                     CRSLAFSLAYQRNDKTLTDTEVDAVHAGVRTKMETRGYILR"
     gene            794..1120
                     /locus_tag="P5219_05370"
     CDS             794..1120
                     /locus_tag="P5219_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26219.1"
                     /translation="MVTISHIEQLADKLVEMVASLRNERDQLRSQYSELKNKFAEKEL
                     ECIRMSKENQRNLEIMEREKLAFQREKSQIEGQMKALYEKLSSLMPEARQPQANGQDA
                     RGERRP"
     gene            1123..1374
                     /locus_tag="P5219_05375"
     CDS             1123..1374
                     /locus_tag="P5219_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26220.1"
                     /translation="MDASLREVTIRIGKKSYFLKTTLDDESIKAITDLSADITREFEG
                     SLDQENLLLLSCLQLAWLLEKLGRKLERSLDDIREKEGL"
     gene            1371..1940
                     /locus_tag="P5219_05380"
     CDS             1371..1940
                     /locus_tag="P5219_05380"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRX26221.1"
                     /translation="MTFSSIFDIAVALIVASFAVRGLFRGLSGEFFSLLGTVGGVMVA
                     WKYSAVPAAWVVSMFPEANPSMVSVGFMVAIYIGVVVLAASICRIVKAFLKFASLTFA
                     DRFFGAVAGVLKGAVLILFLYVGVTTYSPFLPTDWMGASYVMRGADAAWPGIQSFLKK
                     HNLFPENFTLPDLNLPGIFPEKAGGANGG"
     gene            1930..>2480
                     /locus_tag="P5219_05385"
     CDS             1930..>2480
                     /locus_tag="P5219_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HRX26222.1"
                     /translation="MEVREDILRILEIPKVLAEFASCVRGELGLSALGRLRPRGDVKA
                     LEERVALLKSCMSCRDSFGEWPWNSSGCISGLLSEARKSGMLTGEELASVARFLSLAK
                     MTREHLAKHRETYPLFDSLSRQIRDFSEESDALGVVDDKGNLYDSASPELADIRHELE
                     SLRRQIRRSAQNILDNPSLAHML"
BASE COUNT          533 a          703 c          715 g          529 t
ORIGIN      
        1 ccctggaact ggcgaatccc ctgagcgcag aacagtcgaa gatgaggacc acgctcctcc
       61 ccgggctgct gcggagcgtg gagttatccg tccaggcggg atggaaggct cccgtgagaa
      121 tttttgagct cggcagggtt ttcctccccc tggacgaagg cgggcatgaa gaagtggagc
      181 gcatctccgg cctcgtctac ggcggccggg acccccgcct tcctttcggt cccgccggag
      241 cggacgatct tttttccctg aaaggggata tactcgccct cgctgagagc aggggcgttt
      301 cactcgactt tgaacagggc gaagaaccct tcgggcacaa gggacagact gccgtcatca
      361 tgaatgccgg ccgtgctgcg gggtacctcc tgcggctgaa gcctgccatc gagaaggata
      421 tcgagtgctc tcccgtctac gccttcgagc ttgacctgga actcctggtg aaggagtccc
      481 ttccgctctt cagggaaaac tccccgttcc cgccggtgta tcgggatatt tcccttatcg
      541 ttccggtgga cagtcccatg gaggaagtct cggccgcgat ccgcagcgaa ggaggcgaac
      601 tcctcaggag catccgcctc ttcgatatct actcaggaaa gggaataccc gacggctgca
      661 ggagtctcgc tttttccctc gcataccagc ggaacgacaa gacactcacc gatacggaag
      721 tcgatgccgt tcacgccggt gtccggacta aaatggaaac caggggatat attcttcgat
      781 aggaggaagt gccatggtca cgatcagtca tattgaacag cttgccgaca agctcgttga
      841 gatggtggcg tcgctgagaa acgaaaggga ccagcttcgc tcccagtaca gcgaactgaa
      901 gaacaaattt gcggaaaaag agctcgagtg cattcgcatg tcgaaggaaa accagcgcaa
      961 ccttgagatc atggaacggg aaaaacttgc gttccagagg gaaaagagcc agattgaagg
     1021 gcagatgaaa gccctgtacg aaaaactgag ttccctcatg cccgaggcga ggcagcccca
     1081 ggcaaacggc caggacgcca gaggtgaaag gagaccctag gagtggatgc ctcccttcgt
     1141 gaagtgacca tccggatagg gaaaaaatca tactttctga aaaccactct cgatgacgaa
     1201 agcatcaagg ctataaccga cctgtctgcc gatattaccc gggagtttga aggttccctc
     1261 gaccaggaga accttctgct cctgtcctgt ctgcagcttg cgtggctgtt ggagaaactc
     1321 ggaagaaaac tcgaacgatc gcttgatgat attcgggaaa aggaggggct atgacttttt
     1381 cgtccatttt tgacattgcc gttgccctga ttgtggcatc ctttgccgtc aggggactgt
     1441 tccgcggatt gtccggggag ttcttttctc ttctcggaac ggtgggaggc gtcatggtcg
     1501 catggaaata ctcggccgtg cctgcggcct gggtagtctc catgtttccg gaggcaaacc
     1561 cctccatggt gtcggtgggg ttcatggtgg ccatatacat cggcgtcgtg gtactcgcag
     1621 cctcgatctg ccgtattgtc aaggccttcc tcaagttcgc ctccctcact ttcgccgaca
     1681 ggtttttcgg cgcggtcgcc ggagttctca agggggccgt cctcatcctc ttcctctacg
     1741 tgggcgtcac cacctactct ccctttcttc ccaccgattg gatgggggcg agctacgtaa
     1801 tgaggggagc cgatgccgca tggccgggca tacagtcctt tctcaaaaag cataatctct
     1861 tcccggagaa tttcaccctg cctgacctga acctcccggg catctttcct gaaaaagccg
     1921 gaggcgccaa tggaggttag ggaggacatt ctcagaatcc tcgaaatacc gaaggttctt
     1981 gccgagttcg cctcctgtgt caggggcgaa ctcggtttgt cggccctcgg aaggctgagg
     2041 ccccggggag atgtcaaagc ccttgaggag agagtggccc ttttgaaaag ctgcatgtca
     2101 tgcagggact ccttcggcga atggccgtgg aattcctcgg gctgcatttc cggcctgctc
     2161 tctgaagcgc ggaagtccgg catgcttaca ggagaagaac tcgcttccgt ggcccgcttt
     2221 ctctccctgg cgaagatgac cagggagcat ttggcgaagc acagggagac ctatcctctc
     2281 ttcgacagcc tcagccggca gatacgggac ttttcggaag agtcggatgc cctcggagtg
     2341 gtggacgaca agggcaacct atacgattcc gcatcccccg agctcgcgga tatacggcat
     2401 gaactggaga gccttcgccg gcagatacgc cggtcagccc agaacatcct cgacaacccc
     2461 tctcttgccc atatgctgca
//
LOCUS       DAOTHD010000262         2475 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_50255_length_2475_cov_2.810744, whole genome shotgun sequence.
ACCESSION   DAOTHD010000262 DAOTHD010000000
VERSION     DAOTHD010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50255_length_2475_cov_2.810744"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="P5219_05390"
     CDS             complement(<1..647)
                     /locus_tag="P5219_05390"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HRX26223.1"
                     /translation="METLTLFIVSDSTGETAEYVALAAISQFDTDFSKIVRYRYVEGP
                     GKIDEIVSRAAAVPSLVVCTLVDKHTRLALAEKSAAAGIPFVDLLGPMVDALELRLGR
                     KPLQAPGLLRKLDEDYFQKIKAVEFAIQCDDGRSTESLPDADLVILGVSRTGKTPLSM
                     YIANKGFKAANIPLIPEVDPPEIIRKVPSSKIVGLIISAERLIQIRQERLRLLGL"
     gene            complement(741..>2475)
                     /gene="glyS"
                     /locus_tag="P5219_05395"
     CDS             complement(741..>2475)
                     /gene="glyS"
                     /locus_tag="P5219_05395"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HRX26224.1"
                     /translation="GVRYACAEVSEPGGRTLDILPELLPGLIRKLVFPKNMYWDDPTV
                     RFARPIRWILAMADREVIPFEYGGIRSGNATVGHRFMGARNIPIADGADFMDKLYDNY
                     VILDQDKRRQKMLAGIASLEKDLDGSVELDPEVINENLYLVEYPVPFFGSFSRKYLEM
                     PEEVLTTSMKKNQKYFSVRGKNGKLLPYFVGVSNNLASNMAVVREGNERVLRARLEDA
                     AFFWAEDLKNPLAANVERLKNIVYQEKLGSLHDKVMTTRELALRLCGDMGLNDLVPVV
                     DRAAYLAKADLVTNMVYEFPELQGLMGREYALRNGEPEKVARALYDQYLPKAAGSELP
                     GGVPGALIGLAERTHVIVSCHKVGLEPSGSQDPYGLRRAARCINEIIWGLGLDVDVSR
                     LSAQSMNLLDASPETGEKILSFLSQRLLMQVKEKGFGHELASLAISVTGHRPLQVLRF
                     LEVFSKLQGVEWFTNLVTSAVRVRNIISKSGGETGAVDPSLFAKDAETALYGEMQRIL
                     PLVDGAMEKQDWNGLAGYLAELSPFVTAFFDDVLVMDPDERIKSNRIALLGMCSALFL
                     KVGDVGVLKGA"
BASE COUNT          435 a          729 c          811 g          500 t
ORIGIN      
        1 tcgaggccga gaagccggag ccgctcctgg cggatctgga tgagcctctc cgccgagatg
       61 atgaggccga ctattttgga cgacggcacc ttcctgatga tctccggagg atccacctcg
      121 ggaatgaggg gaatgttggc ggccttgaag cccttgttgg caatgtacat ggagaggggg
      181 gtcttcccgg tccgcgaaac gccgaggatg accaggtccg catcggggag ggattcggtg
      241 ctccggccgt cgtcgcactg gatggcgaat tccacggcct tgatcttctg gaagtagtct
      301 tcgtccagct tccgcagcag tcccggagcc tggaggggtt ttctcccgag ccgcagctcc
      361 agggcatcca ccatggggcc gaggagatcg acgaagggga ttcccgccgc cgcgcttttt
      421 tccgccaggg ccaaacgggt atgcttgtcc accagggtgc agacgacgag agaaggaacg
      481 gcggccgccc ttgacacgat ttcgtcaatc tttccgggac cttccacata ccggtaccgg
      541 acgatttttg agaaatcggt gtcgaactgg ctgatggcgg ccagggctac atattccgcc
      601 gtctcgccgg tagaatcgga aacgataaag agggtcaggg tttccaagga cgtccactct
      661 ccttcggttc ggtgatggtg caactctttt gaatgggggg ccgccggaac gggttctccg
      721 ccggcccccc ggagaacctg tcaggccccc ttgagcactc ccacgtcccc caccttgagg
      781 aagagggcgc tgcacatgcc gaggagggct atgcggttgg attttatcct ttcgtcgggg
      841 tccatgacaa ggacatcgtc gaagaaggct gtgacgaagg gcgacagctc ggcgagatac
      901 cccgcgaggc cgttccagtc ctgtttctcc atggcgccgt ccacgagggg aaggattctc
      961 tgcatctccc cgtagagggc cgtctccgca tcctttgcga agagggaggg atcgacggct
     1021 cccgtttccc cgccgctctt ggatatgatg ttccgcaccc gcaccgcaga ggtgaccagg
     1081 ttggtgaacc actccacccc ctggagcttc gagaagactt ccaggaaccg gaggacctgg
     1141 aggggccggt gtcccgtgac ggatatggcg agggacgcga gttcgtggcc gaaccccttt
     1201 tccttgacct gcatgagcag acgctgggaa aggaaggaga ggatcttctc ccctgtctcg
     1261 ggcgatgcgt ccaggaggtt catggactga gccgagagcc gggatacgtc cacgtccagc
     1321 ccgagtcccc agatgatctc gttgatgcac ctggcagccc gccggagacc ataggggtcc
     1381 tgggaaccgc tgggctcgag ccccactttg tggcagctca cgatgacgtg ggtccgttcg
     1441 gcgaggccga tcagggctcc cggcacgccg ccggggagtt cgctgccggc ggccttggga
     1501 aggtactggt cgtagagcgc ccgggccact ttttccggct cgccgttgcg gagggcgtat
     1561 tcccttccca tgagcccctg gagttcggga aattcataga ccatgttggt gacgaggtcg
     1621 gccttggcga ggtaggccgc ccggtccacc acgggcacga ggtcgttcag ccccatgtct
     1681 ccgcagagcc ggagggccag ttccctggtg gtcatgacct tgtcgtggag agagccgagc
     1741 ttttcctggt agacgatgtt tttcagccgc tccacgttgg cggcgagggg gtttttcagg
     1801 tcttccgccc agaagaaggc tgcgtcctcg aggcgggccc tgaggacccg ctcgttgccc
     1861 tcccggacca cggccatgtt ggacgccagg ttgttgctca cacccacgaa gtaggggagg
     1921 agtttgccgt tctttccccg gacggagaaa tacttctggt tctttttcat ggaggtggtg
     1981 agcacctctt caggcatttc caggtacttc ctgctgaagg acccgaagaa gggcacgggg
     2041 tactccacca ggtagaggtt ttcgttgatg acttcggggt caagctccac ggagccgtca
     2101 agatccttct caagggaggc gatgccggcg agcatcttct ggcgccgctt gtcctggtcg
     2161 aggatgacgt agttgtcata gagcttgtcc ataaagtccg ccccatcggc tatggggatg
     2221 ttccgggcac ccatgaaccg gtgccccacg gtggcgtttc cgctccggat tcctccgtac
     2281 tcgaagggga tgacctcccg gtccgccatg gcgaggatcc acctgatggg ccttgcgaag
     2341 cggacggtgg ggtcgtccca gtacatgttc ttgggaaaga cgagcttcct gatgaggccg
     2401 ggcagaagct cggggagaat atcaagggtc ctccccccgg gctcggagac ttcggcgcag
     2461 gcgtagcgca cgccg
//
LOCUS       DAOTHD010000263         2474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_50275_length_2474_cov_3.408020, whole genome shotgun sequence.
ACCESSION   DAOTHD010000263 DAOTHD010000000
VERSION     DAOTHD010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50275_length_2474_cov_3.408020"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /gene="dgoD"
                     /locus_tag="P5219_05400"
     CDS             complement(<1..773)
                     /gene="dgoD"
                     /locus_tag="P5219_05400"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HRX26225.1"
                     /translation="MARIERLDLFKVPPRWLFLKVTTDEGVEGWGEPVVEGRADTVAA
                     AVREMGEYLVGSDPTKIEDLWQALYRGGFYRGGPILMSAISGIEQALWDISGKMAGLP
                     VHRMMGGAVRDKMRVYSWIGGDRPDDVVTEAKKRVDAGMTAVKMNGTEEMAWIDSFTR
                     IEEVISRVQALRDAFGHSLDIAVDFHGRVHLPMARVLMKELEPFRLMFIEEPVLVEHR
                     EAFRELRRHTVTPIATGERSFSRWDFKDLITGGGVDILQP"
     gene            complement(779..1435)
                     /gene="eda"
                     /locus_tag="P5219_05405"
     CDS             complement(779..1435)
                     /gene="eda"
                     /locus_tag="P5219_05405"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HRX26226.1"
                     /translation="MNIRKYEVLSRIHDLGVVGIIRTPDTAGGIASAEAVFQGGITVL
                     EVSTTFPGALDIMRSTAASHKSSGLILGAGTVADSETAKLCIDAGAEFIVSHCFSEEV
                     ARLCNRYGVAYMPGVGTVTEIVRAMEWGADVVKAFPGEVLGPKFIKAVHGPLPNAQIM
                     PIGGVSPNNLEDWFLAGAFAVGLGSALVKPEGREGDYPAIRQTAEEIVSRIARIRTHV
                     "
     gene            1532..2185
                     /locus_tag="P5219_05410"
     CDS             1532..2185
                     /locus_tag="P5219_05410"
                     /inference="COORDINATES: protein motif:HMM:NF019947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRX26227.1"
                     /translation="MKQVQEEFAFFESLMKGLTAIFGTNCEVVLHDLTDSYESTVVMI
                     ENGHVTDRRVGDCGSNLGLEVLRGTVRNGDKYGYFTNTRDGRVLRSSSVYIRDGEGKV
                     IGCLCVNFDVSNLMVADKTIRSLISNGEGEKEEEFFVNDVNQLLDALLKKAMEEVGKP
                     VSYMTRDDKIRVVKYLDQKGAFLITKSGNRICQFLDISKFTLYSYLDEVHSEIQPSK"
     gene            2200..>2474
                     /locus_tag="P5219_05415"
     CDS             2200..>2474
                     /locus_tag="P5219_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26228.1"
                     /translation="MTRDIYRELGVRTVINAAGTYTMVGGSRMSEETLSAMASAARNH
                     VVIKELQQKVHERLASITRNEAAFVTNGAAAALHIAGASAIARKWGR"
BASE COUNT          503 a          745 c          737 g          489 t
ORIGIN      
        1 ggctggagaa tgtccacccc tccgccggtg atgagatcct tgaagtccca gcggctgaag
       61 ctacgctctc cggtggctat gggggtgacg gtgtgccgcc ggagttcccg gaaagcctcc
      121 cggtgttcca cgagcacggg ctcctcgatg aacatgaggc ggaagggctc gagctccttc
      181 atgagcaccc ttgccatggg caggtggacc cggccgtgga agtcaacggc gatgtccaga
      241 gaatggccga aagcgtcccg gagggcctgg acacgggaaa tgacttcctc gatcctggtg
      301 aagctgtcga tccaggccat ttcctccgtg ccgttcatct tgacggcggt catccccgca
      361 tcgacccgct tctttgcctc cgtcacgaca tcgtcggggc ggtctccgcc gatccacgag
      421 taaaccctca ttttgtcccg cacggcgccg cccatcatcc tgtggacggg aaggccggcc
      481 atcttccctg aaatgtccca gagggcctgc tcgatgccgg agatggcgct cataagaatg
      541 gggccgccgc gataaaatcc gcccctgtag agggcctgcc agaggtcctc tattttcgtc
      601 gggtctgacc cgacgaggta ttctcccatc tcccgcaccg ctgcggcaac ggtgtcggcc
      661 cggccttcca cgaccggttc gccccagcct tccacgccct cgtccgtagt gactttcaga
      721 aaaagccacc tgggcggcac tttaaaaaga tcgagccgct cgatcctggc catggcgttt
      781 acacgtgggt gcggatccgg gctatgcggg agacgatctc ttcggccgtc tgccggatgg
      841 cggggtagtc cccctcccgg ccttccggct tgacgagggc gcttcccagg ccgacggcga
      901 aagcccccgc gaggaaccag tcttccaggt tgttcgggct gacgcctccg atgggcatga
      961 tctgggcgtt cgggagaggc ccgtggaccg ccttgatgaa tttggggccg agaacttctc
     1021 cgggaaaggc cttcaccacg tccgctcccc attccatggc ccggacgatt tcggtcaccg
     1081 tgcccacgcc gggcatgtag gcaacgccgt agcggttgca cagcctcgcc acttcctcgc
     1141 tgaaacagtg ggagacgatg aactcggccc cggcatcgat gcagagcttt gccgtctccg
     1201 aatcggccac cgttccggcg cccaggatga ggccgctgct cttgtgggag gcggcggtgc
     1261 tcctcatgat gtcaagggct cccgggaagg tggtggaaac ttcgaggacg gtgattcctc
     1321 cctggaacac cgcctcggcg gaggcgatgc cgcccgccgt gtccggcgtc cggataatgc
     1381 cgacgacgcc gaggtcatgg atgcggctca gaacttcata cttgcggatg ttcatgatgt
     1441 gctcactcct tgatttattg acgttagagt attcagacag tataataaca taaattcaga
     1501 caaactaaat atttttttgg aggcgttcct tttgaaacaa gtccaggaag aattcgcctt
     1561 tttcgagtcc ctcatgaaag gactcaccgc tatcttcggc acgaactgcg aagtggttct
     1621 ccatgatctc accgacagct acgaaagcac cgtggtcatg atcgagaacg gccacgtcac
     1681 agaccggcgc gtgggcgact gcggaagcaa cctcggtctg gaagtactcc gcggtaccgt
     1741 gcgcaacggg gacaagtacg gttattttac caacacccgg gacgggaggg tactccgttc
     1801 atcgtccgtg tatatccgcg acggcgaagg aaaggtcatc gggtgcctgt gcgtaaactt
     1861 cgacgtctcg aacctcatgg tcgccgacaa gaccatccgg tccctcattt ccaacggcga
     1921 gggggagaag gaagaggaat tcttcgtcaa cgacgtgaac cagcttcttg acgccctgct
     1981 gaagaaagcc atggaagaag tgggcaaacc tgtttcctac atgaccaggg acgacaagat
     2041 cagggtggtg aaatacctcg accagaaagg cgctttcctc atcaccaagt cgggaaaccg
     2101 gatctgccag tttctcgata tttccaagtt tacgctctac tcctatcttg acgaggtcca
     2161 ttcagaaatt caaccttcaa aataaggcgg cgatgcgcag tgacacggga catttacagg
     2221 gaactcggag ttcggacggt catcaacgcg gcggggacct acaccatggt ggggggctcc
     2281 cgcatgagcg aggagacgct ctcggcaatg gcgtcggcgg cgagaaacca cgtagtgata
     2341 aaggagctgc agcagaaggt tcatgaacgc ctcgcctcca tcacgagaaa cgaggcggct
     2401 tttgtcacga acggcgcggc cgcggccctg cacatcgcgg gagcttcggc catcgcccgg
     2461 aaatggggcc gtca
//
LOCUS       DAOTHD010000264         2471 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_50339_length_2471_cov_3.039321, whole genome shotgun sequence.
ACCESSION   DAOTHD010000264 DAOTHD010000000
VERSION     DAOTHD010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50339_length_2471_cov_3.039321"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /gene="hypD"
                     /locus_tag="P5219_05420"
     CDS             <1..914
                     /gene="hypD"
                     /locus_tag="P5219_05420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HRX26229.1"
                     /translation="GCPVCVTDQGEIEAALSLLDRGAVVAAYGDMFKVPCKGGSLASK
                     RSGGLDARVVTSAMEVLSIAVENPDREVVFLGVGFETTAPATAALVSEAARRGVPNLS
                     LLSFHKRTPPAVELLAADPDLRLSGFLLPGHVCVILGHDPFRFLPEKYGLPGVIAGFE
                     PDQILLGLAEIVRQHEQGAPALRSVYGTAVKKEGNTKALGLLEEVFRHRDAPWRGIGT
                     IPDSGLGLRKAYESFDAEKRFSLSLLPGAPPAGCRCGEVLTGKIIPPECPLFRSACTP
                     LSPVGPCMVSGEGTCGAYFRFYKGDRS"
     gene            911..1909
                     /gene="hypE"
                     /locus_tag="P5219_05425"
     CDS             911..1909
                     /gene="hypE"
                     /locus_tag="P5219_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HRX26230.1"
                     /translation="MKDTAITLGHGSGGRQSADLVSHLLESFGKRGIGPEMEDAALLP
                     GGYAMTMDGFTVSPRRFPGGDIGHLCVCGCANDLAVRGASPDFLGISLVLEEGISPEE
                     VSSYMESAASTCAELGMTLAAGDTKVVPSGAVEGMIITGCAFGQIRTPRTLSMSSIRE
                     GDALLVTTSVGRHGAAIASARFNLPVEGLLSDCAPLWPALSPLLHLEGLRCMRDCTRG
                     GLGTVLCEWAEASELHFSLDEDSIPVSFQVKSLCDVLGFDPLYMACEGCAALAVAPSD
                     TEETLRILRQSPLCGDAAVIGRVSSAEPKGMVSMRTSIGGERLVDMPWGEILPRIC"
     gene            complement(1956..>2471)
                     /locus_tag="P5219_05430"
     CDS             complement(1956..>2471)
                     /locus_tag="P5219_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HRX26231.1"
                     /translation="PANYPDECPGQGMAVLEKNGMRLAIANLQGRVFMPPIDCPFRKA
                     DALLAAAGQVPSLLDFHAEATSEKKALAHYLDGRAAVVIGTHTHVQTADEDILPGGTA
                     CITDAGMTGGHRSSIGMKMEGVLPKFLTGMPSRFEVSGEGLALNGVAVTLDDETGRAV
                     DILRINEKLSH"
BASE COUNT          421 a          791 c          783 g          476 t
ORIGIN      
        1 cgggttgccc cgtgtgcgtc accgaccagg gtgagatcga agccgccctt tctcttctcg
       61 acaggggggc cgtcgtcgcc gcctacgggg atatgttcaa ggtcccctgc aagggaggat
      121 cccttgcgtc aaagcggtcc gggggactgg acgccagggt tgtcacaagc gccatggagg
      181 tgctgtccat cgccgtggag aatcccgatc gggaggtggt ttttctcggc gtagggttcg
      241 agaccaccgc cccggctacg gcggccctgg tgtccgaagc tgcccggaga ggcgtcccca
      301 acctttcgct actttcgttc cacaaacgga cgccgccggc ggtggagctt ctcgccgcgg
      361 acccggatct ccggctttcg ggatttcttc ttcccggcca tgtatgcgtc atcctcggcc
      421 acgatccctt ccgtttcctt cccgaaaagt acggccttcc cggcgtgatc gccggatttg
      481 agccggacca gattctgctg ggcctcgcgg aaatcgtcag acagcacgag cagggggccc
      541 ctgccctccg ttcggtctat ggcacagcgg tgaaaaagga aggcaatacc aaggccctcg
      601 gactgcttga agaggtcttc cggcaccggg acgccccgtg gaggggtatc gggaccattc
      661 cggactcggg gctgggcctg agaaaggcgt atgaatcctt tgacgcggaa aagcggttca
      721 gcctctccct gctcccgggc gccccacctg ccggatgccg gtgcggcgaa gtgctgacgg
      781 gaaagatcat ccctccggaa tgccccctgt tccgctcggc atgcaccccc ctgtctcccg
      841 tgggcccctg catggtgtcc ggggagggaa cctgcggggc gtacttccgt ttttataaag
      901 gagacaggtc atgaaggaca cagcaatcac cctgggacac ggaagcggcg ggcggcagag
      961 cgccgatctc gtatcgcacc tcctcgaatc cttcgggaag cggggcatcg gccccgagat
     1021 ggaggacgcg gccctccttc cgggaggcta cgccatgacg atggacggct ttaccgtctc
     1081 gccccggaga tttcccggcg gcgacatcgg tcacctgtgc gtctgcggct gcgcaaacga
     1141 tctggccgtc cggggagcga gccctgattt cctcggcata agtctcgtcc tcgaggaggg
     1201 aatttcgccg gaagaggtct cctcctacat ggagagcgcc gcctcgacct gcgccgaact
     1261 cggcatgacc ctcgccgccg gggacaccaa ggtcgtgccc tctggagcgg tggagggaat
     1321 gatcatcacg ggctgcgcct tcgggcagat caggacgccc cggacgctct ccatgagctc
     1381 catacgggag ggcgacgctc ttctcgtcac cacgtccgtg ggaaggcacg gagcagccat
     1441 cgcgtccgcc cggttcaacc ttcccgtgga aggactgctg agcgactgcg cacccctgtg
     1501 gccggccctg agcccccttc ttcacctcga agggctgcgg tgcatgaggg actgcaccag
     1561 gggcgggctg ggaacggtgc tctgcgaatg ggcggaagcc tcggaactcc acttctccct
     1621 cgatgaggac tccataccgg taagcttcca ggtgaagtcc ctctgcgacg ttctcggctt
     1681 cgatcccctc tacatggcct gcgagggatg cgccgccctt gcggtggccc cttcagacac
     1741 cgaagaaacc ctgcgcatcc tcagacagtc gcctctctgc ggagacgcgg ccgtcatcgg
     1801 ccgcgtctcc tccgcggaac cgaagggaat ggtttccatg aggacctcca ttggaggaga
     1861 gcggctggtg gatatgccct ggggggagat tcttcccagg atctgctgaa agacaaaaat
     1921 gagcggggga gccttgaggc ctcccccgct cattttcagt gagacagttt ttcgttgatt
     1981 ctgagaatgt ccacggcccg gcccgtctcg tcatccaggg tgacggccac cccgttcagg
     2041 gccagcccct ctccggagac ttcaaaccgt gacggcatgc cggtgagaaa cttcgggagc
     2101 acgccttcca ttttcattcc gatggaactt ctgtgccctc ccgtcattcc tgcgtcggta
     2161 atgcaggccg tgcctccggg aagaatgtcc tcgtcggcgg tctgaacatg ggtgtgggtt
     2221 ccgatcacca cagcggcccg gccgtcgaga taatgggcca gcgccttttt ttccgatgtg
     2281 gcttcggcgt ggaaatcgag aagagaggga acctgcccag ctgcggcgag aagggcgtcc
     2341 gccttcctga agggacagtc gatggggggc atgaaaaccc ttccctggag attggcaatg
     2401 gcgagacgca tgccgttttt ctccagcacc gccattccct gtccggggca ttcgtccgga
     2461 tagttcgccg g
//
LOCUS       DAOTHD010000265         2469 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_50388_length_2469_cov_3.144573, whole genome shotgun sequence.
ACCESSION   DAOTHD010000265 DAOTHD010000000
VERSION     DAOTHD010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50388_length_2469_cov_3.144573"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="P5219_05435"
     CDS             <1..652
                     /locus_tag="P5219_05435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HRX26232.1"
                     /translation="SEDGSIKLVSAPFQLGFRGKFLIGDLSLANVMSETYMSTEEGAL
                     QVTDAPLDAAIVGEGYFTVQDGAGNIFYTRSGHFQRSPEGQLVTHDGLMVLGDGGPVE
                     FGDAARVAIGGGGQIIADGEAVDVLQVVTFPNPTYLRQAGRTTLAATENSGAPAALEP
                     EQITLEPGALEMSNVNVVEEMVRMVEAQRAYEASSKTLMTHDELTGKLITSFGRTS"
     gene            669..1457
                     /gene="flgG"
                     /locus_tag="P5219_05440"
     CDS             669..1457
                     /gene="flgG"
                     /locus_tag="P5219_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HRX26233.1"
                     /translation="MLRALWTSASGMIAQQTNLDVTTNNLANVNTAGFKRKRADFADL
                     LYQINREPGAPVEPASMVPNGVQVGLGTRVVGTSRFMGQGNLQVTEQPLDIAIEGDGF
                     YQVLLPNGEIAFTRAGNWNVDSDGQVVNEDGLLLEPALVIPEDATEIMVSPEGLVTVS
                     IAGQEEAEEVGQIELVRFVNPGGLRAMGKNLFVETPASGAPIAGLPGEEGLGQLHQGV
                     LEMSNVQVVDEMVSLIIAQRAYEANSKGVQTADELLRIANGLKR"
     gene            1466..2386
                     /gene="flgA"
                     /locus_tag="P5219_05445"
     CDS             1466..2386
                     /gene="flgA"
                     /locus_tag="P5219_05445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HRX26234.1"
                     /translation="MNGKRKRQRDAFRSRLFFPLLAAFLILFTEVPVLAADLVLEMGA
                     TPVVTSDPFPLSEAALLTGEPELVRKAGAVRLSVPPNGILTRDALLVAISEQGIGGIR
                     LKLIMPPEVAVSLDESLSAVIKRLSGWEWQVEAEPLGDIPPGKPVGPLSIAPGTGSVT
                     LKYDDGTGSERSLAVRLSWSRPAVVAARPVERGKVLDRDDVTVQSVRILRSTPLASAV
                     EDVLGTVARRNLSAGEPIALNLFSSAPIIQRGDPVIISVRKAGFRIEVRGEALDAGCE
                     GESIRVRNLQSRKVIQAVAVAPGRAEVQSE"
BASE COUNT          499 a          779 c          769 g          422 t
ORIGIN      
        1 ttccgaagac ggatccatca agctcgtgtc cgcccccttc cagctcggct tcaggggaaa
       61 gttcctcatc ggcgacctca gcctcgcgaa cgtcatgtcc gaaacttaca tgagcaccga
      121 ggagggggcc ctccaggtga ccgatgcccc cctcgacgcg gcgatcgtcg gcgaaggcta
      181 ctttaccgtc caggacggcg ccggaaacat cttctatacc aggtcgggcc attttcagcg
      241 gagccccgag gggcagctcg tcacccatga cggcctcatg gttctgggcg acggcggccc
      301 ggtggagttc ggagacgcag cccgggtggc catcggcgga ggcggccaga tcatcgccga
      361 cggagaagcg gtcgacgtgc tccaggtggt gacctttccc aatcccacct acctccggca
      421 ggcgggcagg acgaccctcg cggccacgga gaattccggg gctcccgcag ccctggagcc
      481 ggagcagata acccttgaac ccggtgcgct ggagatgtcc aacgtcaacg tggtggagga
      541 aatggtgcgg atggtggaag cccagcgggc ctacgaggcg tcgtcaaaga ccctcatgac
      601 ccatgacgaa ctgacaggca agctgatcac gtccttcggc aggaccagct agcgggaggg
      661 agaaaaatat gctcagagcc ctgtggacaa gcgcaagcgg aatgatcgcc cagcagacca
      721 acctggacgt cacgaccaac aacctcgcca acgtgaacac cgccggattc aaaagaaaac
      781 gggcggattt cgccgatctt ctgtaccaga tcaaccggga accaggcgca cccgtggaac
      841 cggcctccat ggtacccaac ggcgtgcagg tcggccttgg aacccgtgtt gtgggaacct
      901 cccgcttcat gggccagggc aacctccagg taaccgaaca gcccctcgac atcgccatcg
      961 agggagacgg attttaccag gtcctcctcc ccaacggcga aatagccttc accagggcgg
     1021 gcaactggaa cgtggattcc gacggccagg tggtcaacga ggacggtctt ctcctcgagc
     1081 cggcccttgt catccccgag gacgccacgg agatcatggt cagccccgaa ggcctcgtca
     1141 cggtcagcat cgcgggccag gaggaggcgg aggaagtggg ccagatcgaa cttgtccggt
     1201 tcgtcaatcc cggaggcctc agggcgatgg ggaagaacct cttcgtcgag acgcccgcca
     1261 gcggagctcc catcgcggga cttcccgggg aagaaggtct cggccagctt caccagggag
     1321 tcctcgaaat gtccaacgtc caggtggtgg acgaaatggt ctccctcatc atagcccaga
     1381 gggcctacga ggcaaactca aagggtgttc agacggcgga cgaactgctc cgcatcgcca
     1441 acgggctgaa gaggtaagcg ccgccgtgaa cggaaaaaga aaacgacagc gggatgcatt
     1501 tcggtcccgc cttttttttc ctctcctggc cgccttcctg atcctcttca ctgaagttcc
     1561 cgtccttgcc gccgaccttg tccttgaaat gggggcaacc ccggtcgtaa cgtcggatcc
     1621 cttccccctc tccgaagcgg cccttctcac cggtgagccg gaacttgtcc ggaaagcggg
     1681 ggccgtgagg ctttccgttc caccgaacgg aatcctcacc cgggatgccc tccttgtcgc
     1741 catatcggaa caggggatcg gaggcatccg gctcaagctc atcatgccgc cggaagtggc
     1801 ggtctccctt gacgagtctc tttccgcggt catcaaacgg ctttcaggat gggaatggca
     1861 ggtggaagcg gagcctctcg gagacatccc tcccggaaag cccgtcggtc ctctttccat
     1921 cgctccggga acgggatcgg tcacgctgaa atacgacgac ggcaccgggt ccgaacggtc
     1981 ccttgcggtg aggctctcct ggagccgtcc tgccgtggtg gcggcccgac cggtggagcg
     2041 gggaaaggtc ctggacaggg acgacgtgac ggtccagtcc gtgaggattc tccgctcgac
     2101 gcccctggcg tcggcggtgg aggatgtcct cggcacagtg gcccggagga atctttccgc
     2161 cggtgagccc atagccctca acttattctc ttccgcgccg ataattcaaa gaggggatcc
     2221 cgtcatcatc tccgtcagga aagcgggatt ccgcatagaa gtccggggag aggctctcga
     2281 cgccgggtgc gaaggagagt ccatccgcgt acggaacctg cagagcagaa aggtcatcca
     2341 ggcggtggca gtagcgccgg gacgtgcgga ggtgcaatca gagtgaaaaa aataatcctt
     2401 gcggcagcag cgctgcttct tttgttcggg gcaacgccct ttcaggccca ggcccgttcc
     2461 ctgtgggac
//
LOCUS       DAOTHD010000266         2462 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_50569_length_2462_cov_3.208558, whole genome shotgun sequence.
ACCESSION   DAOTHD010000266 DAOTHD010000000
VERSION     DAOTHD010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50569_length_2462_cov_3.208558"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1544
                     /locus_tag="P5219_05450"
     CDS             <1..1544
                     /locus_tag="P5219_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRX26235.1"
                     /translation="QPAFEKEPESEEKDPHALPEIEFNELEGTEKKVRGAAFPPPLDL
                     FGPKQLLDSQESNETVREQAASIIATLGDFGVSAEMADVVIGPTVIQFQMQLAPGIKV
                     SKVAGLSNDLAVALTVPSLRIEAPIPGKPYVGVEIPNPKRRGISIRTVLESQIFQDNE
                     YDLPLPMGVRVDSRPLVVGLEDLPHLLVAGTTGSGKSVFVTSCITGLCSYRSPEELRL
                     ILIDPKRVEMSMYENLPHVLAKPVVSPKKAVQALAWAVREMEHRYEIFARARVRNLKS
                     YNQCVLPKAQLPYIVIVVDELADLMFTAQKEVEDYICRLAQMARATGIHLILATQRPS
                     VNVITGLIKANVPARVAFTLPSQTDSRTIIDISGAERLLGKGDMLFVSSRYPRPLRVQ
                     SPFIDDGKSIEIINYLRNVFGDPEYVDIEDQGGESPSGGDTSYADDPLLEEAVDIVLD
                     TGIASASRLQRQLRVGFTRAARLIDTMEQIGIVGPPEGSKPREILVDDDRAREMLRST
                     SGLGD"
     gene            1546..2268
                     /locus_tag="P5219_05455"
     CDS             1546..2268
                     /locus_tag="P5219_05455"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HRX26236.1"
                     /translation="MGEIVLPGVRAVVSAEEPGEPLAIVAGGREPSPQWLREFLPGKD
                     LWCADSGLIPCMKAGFTPSRLLGDGDSTPDTLWEKVVSAGTTAVESFPADKDYTDLQL
                     AFFRAAGEERRSVIVSGCWGGRFDHLWSAVHSALWAAERGVRVLAFADHLEILFLVYG
                     GEEWILEITDVRYSVLSLLPLGGECGGVFLEGTKWTLNNAVLVPWRPFAVSNSLREKE
                     KPRISVREGILGVYLGTGEKAD"
     gene            complement(2270..>2462)
                     /gene="rpmB"
                     /locus_tag="P5219_05460"
     CDS             complement(2270..>2462)
                     /gene="rpmB"
                     /locus_tag="P5219_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRX26237.1"
                     /translation="ARVCECCGRGPATGNAVSHSNRHTRRRWLVNLRSVKADVGGGES
                     LRMRVCTRCLKAGRVKRAV"
BASE COUNT          512 a          699 c          751 g          500 t
ORIGIN      
        1 gacagccggc ctttgaaaaa gagccggagt cagaggagaa ggacccccat gcccttccgg
       61 agatcgaatt caacgagctg gagggaacag agaaaaaggt gcgtggtgcc gctttccccc
      121 ctcccctgga tcttttcgga ccgaagcagc ttctcgattc ccaggagtcc aatgaaacgg
      181 tacgggagca ggcggcttcc atcattgcca ccctcggtga tttcggcgtg agtgccgaga
      241 tggcggacgt ggtcatcggc ccgacggtca tccaattcca gatgcagctg gctcccggaa
      301 taaaagtcag caaggtggcc ggtctgagca atgacctggc ggttgccctg accgtcccct
      361 ccctgcggat tgaagcgccg ataccgggaa agccctacgt gggagtggag atacccaatc
      421 cgaagaggcg gggcatatca atccgtaccg tattggaatc ccagatattt caggacaacg
      481 agtacgacct gcctctgccc atgggcgtga gggttgattc gcggcccctc gtggtgggcc
      541 tcgaggatct gccccacctt ctggtagccg ggacgaccgg gtccggcaag agcgttttcg
      601 tgacgagctg catcaccggt ctctgttcat accgctcgcc cgaggaactc cggcttatcc
      661 tgatcgaccc gaagagagtg gaaatgagca tgtacgaaaa tctcccccat gtactggcca
      721 agccggtggt ctcccccaag aaggcggtgc aggccctcgc ctgggcggta cgggagatgg
      781 agcacaggta cgagatcttc gcccgagcca gggtaagaaa cctgaagtcc tacaaccagt
      841 gcgttctgcc gaaagcgcag cttccgtaca ttgtgatcgt ggtggacgag ctggctgatc
      901 tcatgttcac cgcccagaag gaagttgagg attacatctg caggcttgcc cagatggcca
      961 gagcgacagg gattcacctt atcctcgcta cccagcgtcc atcagtgaac gtcatcaccg
     1021 gactcataaa ggcgaatgtt cccgcgaggg ttgcctttac cctcccgtca cagaccgact
     1081 cccggaccat cattgatatt tccggtgcag agaggcttct cgggaagggg gacatgctct
     1141 tcgtcagctc gagatacccg aggcccctca gagtgcagtc cccgttcatc gatgacggaa
     1201 agagcatcga aataatcaac tacctcagga acgtcttcgg ggatcccgaa tacgtggata
     1261 tcgaagacca gggaggagaa tccccttcag gaggagatac gtcatacgct gacgatcccc
     1321 ttctggagga ggccgttgat atcgttctgg atacgggcat cgcttcggca agcaggctgc
     1381 agcgccagct gcgggtcggg ttcacccggg ccgcgcggct gatcgacacc atggagcaga
     1441 taggcatagt agggcccccg gaaggctcaa aaccgagaga aatccttgtg gatgacgaca
     1501 gggcaaggga aatgctccgg tccacctccg gcctgggtga ctgacgtggg ggagattgtc
     1561 cttccgggag tgcgggcggt cgtcagcgcc gaagagcccg gtgaaccatt ggccattgtt
     1621 gccggcggga gggaaccttc gccgcaatgg ctgcgggagt tccttcccgg aaaggatctg
     1681 tggtgtgccg attcagggct tattccctgc atgaaggcag ggttcacccc ttccaggctt
     1741 ctcggagacg gtgacagcac gccggatacc ctctgggaaa aggtcgtgtc cgccggcaca
     1801 acggcagtcg agtcttttcc cgccgacaag gattataccg atctccagct cgccttcttt
     1861 cgggctgccg gggaagaacg gcggtcagtc attgtttccg ggtgctgggg cgggaggttc
     1921 gatcacctct ggtccgcagt tcattccgct ctctgggctg ctgaaagggg agtcagggtt
     1981 ctcgcttttg ccgatcacct ggagattctc ttcctggttt acggcgggga ggaatggata
     2041 ctggaaataa cggacgtcag gtattccgtc ctttctcttc tgcctcttgg aggtgaatgc
     2101 gggggagtat ttcttgaagg gacgaaatgg accctgaaca acgcggttct tgttccgtgg
     2161 cggccttttg ccgtcagcaa cagcctgcga gaaaaagaaa agcccagaat ctccgtcagg
     2221 gaagggatcc tgggcgttta cctcgggaca ggagaaaaag cggactgaat cagacagccc
     2281 gcttcactct tccggctttg aggcatcgtg tgcagactct catcctgaga gactcgccgc
     2341 cgccgacatc ggctttcaca ctccggagat tgacgagcca ccgtctgcgg gtgtggcggt
     2401 tggaatggct tacggcgttc cctgtcgcag ggccccttcc gcagcattca catactctgg
     2461 cc
//
LOCUS       DAOTHD010000267         2460 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_50632_length_2460_cov_3.265281, whole genome shotgun sequence.
ACCESSION   DAOTHD010000267 DAOTHD010000000
VERSION     DAOTHD010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50632_length_2460_cov_3.265281"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="P5219_05465"
     CDS             <1..300
                     /locus_tag="P5219_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRX26238.1"
                     /translation="EGNGKQQPPKLGFVLRNLSMSEKSALKGRTGAVILEIKPGGLAE
                     AMKMQAGDIILFCNGKAVPSVEGFSALLSEGENNFLLLRNGKEITVGISTVTESF"
     gene            469..1761
                     /locus_tag="P5219_05470"
     CDS             469..1761
                     /locus_tag="P5219_05470"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF002823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HRX26239.1"
                     /translation="MDDLVGRFPLIEDMKNGKEVFWLNPGLDNRLPEEMTTADIADAS
                     ARLARFAPYIMRAFPETVSSGGIIESPLKDIPEMKTFLEEKSGGTLSGRLLLKCDSEL
                     PISGSIKARGGIYEVLCFAEKVALESGLLKSDGDYAALDEDRFRELFSRYAVSVGSTG
                     NLGLSIGIMSARLGFRVTVHMSADARQWKKDLLRQKGAKVAEYRGDFQMAVAEGRRQA
                     EGDPRCHFVDDENSRALFLGYAVAAERLERQLAGMGITVDGGHPLFVYLPCGVGGGPG
                     GVTFGLKHVFGEHVHCFFAEPVQSPCMLLGILTGRHDGICVQDIGLSGKTSADGLAVG
                     RPSKLVGRLVGNLLDGLFTVSDSRMEELVRILYAKEGVFVEPSATAGFPGFSTVQQRG
                     NYRERFSPASMERSVHIVWATGGSMVPEEERKKYLAEP"
     gene            complement(1793..>2460)
                     /locus_tag="P5219_05475"
     CDS             complement(1793..>2460)
                     /locus_tag="P5219_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26240.1"
                     /translation="GKVTGKAKGSLQASGTFIPQYSLAGKGSLSVSAGAVTGFRNADI
                     AARLYGTTGIRYARTSIPFRLETGRIILEKGSRADAPGNDPIYKFLAAQGSVGPRGAL
                     NLNCAGNINMKLFNAIRGGAAGTLSGGSLEDALKGLLGGLKKGMAEEDFRDTTFTVRG
                     TLDNPSITNLKTAPPPPKQTAPAQAPAQKPAPKPAPPAQPLKPKTPEDILKEKLLESI
                     FKK"
BASE COUNT          472 a          637 c          792 g          559 t
ORIGIN      
        1 gaaggcaatg ggaaacaaca gcccccgaaa ctcgggtttg tgctgagaaa cctgtccatg
       61 agtgaaaagt cggccctgaa gggacggaca ggagcggtca tcctcgagat caagcctggc
      121 gggctcgccg aggcgatgaa gatgcaggcc ggggacatta tccttttctg caacgggaaa
      181 gcggtgccgt cggtggaagg tttttcagcc cttctctcgg agggggagaa caacttcctt
      241 ctcctgagga acgggaagga aatcaccgtt ggcatcagta ccgttacgga atctttttga
      301 gtttgcagat cccggctgtt ctccgggagg tcttctgaag tctgttcttt gctttattga
      361 gggagctttt ccgtggaggg tattttgtac ggtgttcctg tgtgcccgcc ggagaaactg
      421 caccttgtcg gaacagaacc ggaacaagca ggaagggaga aggtattcgt ggatgatcta
      481 gtgggcagat tcccgctgat tgaggatatg aagaacggaa aagaggtttt ctggctgaac
      541 ccggggctgg acaacaggct ccccgaagaa atgacaacgg ccgatattgc agatgcctcg
      601 gcccggctgg cccgtttcgc cccgtacatc atgcgggcct ttccggaaac agtttcatca
      661 gggggaataa tcgagtcgcc gctgaaggac attcccgaga tgaagacctt cctggaggaa
      721 aaatcggggg gtactctttc cggaaggctg ctgctcaagt gcgacagcga gcttcccatt
      781 tccggatcca tcaaggcccg gggaggaatc tacgaggtcc tctgctttgc cgagaaagtt
      841 gcccttgaaa gcggactcct gaaatccgac ggcgattatg ccgccctgga tgaggaccgg
      901 ttccgggagc tgttttcccg gtatgccgtc tctgtgggtt ccacggggaa cctggggctg
      961 agcatcggga tcatgagcgc ccggctcggt ttccgggtga cggtgcacat gtccgccgac
     1021 gcgcggcaat ggaaaaaaga ccttctccgg cagaagggcg ccaaggttgc agagtatcgc
     1081 ggggattttc agatggccgt cgccgaaggt cggcggcagg cggaagggga tccccgctgt
     1141 cactttgtgg atgacgagaa ttccagggca ctgttccttg ggtatgccgt ggcagcggag
     1201 aggctggagc ggcagcttgc cggaatggga atcaccgtgg acggcggcca tcccctgttc
     1261 gtgtacctgc cctgcggcgt gggcggggga cccggcgggg tgaccttcgg cctgaaacat
     1321 gttttcgggg agcacgtgca ctgtttcttt gccgaaccgg ttcagtctcc ctgcatgctg
     1381 ctgggtatcc tgaccggacg gcacgacggg atctgcgtcc aggacatcgg actttccgga
     1441 aagaccagcg cagacggcct cgcggtggga agaccgtcaa agcttgtggg caggctggtg
     1501 ggaaatcttc tcgacgggct tttcaccgtt tccgacagcc gcatggaaga gctcgtgcga
     1561 attttgtatg caaaagaagg cgtttttgtc gagccttcgg cgaccgccgg atttccgggg
     1621 ttttcgaccg tccagcagcg cgggaattac agggagaggt tttctccggc ttcgatggag
     1681 cgttctgtcc atattgtctg ggcgacggga ggcagcatgg ttcctgaaga agaaagaaaa
     1741 aaataccttg cggagcccta aggcgaaaat aaaggagccc tttcgggctc ctttatttct
     1801 tgaagatcga ttcgaggagt ttttccttca gaatgtcctc gggagttttg ggtttcagcg
     1861 gctgtgccgg aggtgcgggt ttcggtgccg gtttctgcgc cggagcctgc gcgggagccg
     1921 tctgcttggg aggcggcggt gcggttttca ggttggttat ggacgggttg tccagcgttc
     1981 cccggacggt gaaggtggtg tccctgaagt cttcttccgc cattcccttt ttgaggccgc
     2041 ccagaagtcc tttcagggca tcctccagag agccccctga gagagtcccg gccgcacccc
     2101 ctcttatggc gttgaagagc ttcatgttga tgtttcctgc gcagttcagg ttcagcgctc
     2161 ccctggggcc tacggaaccc tgggcggcaa ggaatttata tatgggatca ttccctgggg
     2221 catcagcccg ggatcccttt tccaggataa ttctcccggt ttccaggcgg aagggtatgc
     2281 tcgttcgggc atagcgtatt ccggtggttc catagagacg agccgctata tccgcattac
     2341 ggaagccggt cacggctccg gcactcactg acagggatcc ttttcccgcc agtgagtatt
     2401 ggggaatgaa tgtgcccgag gcctggaggg atccctttgc ctttcccgtc acctttccgc
//
LOCUS       DAOTHD010000268         2459 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_50674_length_2459_cov_2.432612, whole genome shotgun sequence.
ACCESSION   DAOTHD010000268 DAOTHD010000000
VERSION     DAOTHD010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50674_length_2459_cov_2.432612"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="P5219_05480"
     CDS             <1..190
                     /locus_tag="P5219_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HRX26241.1"
                     /translation="AGEFGVLWGVVAGFLHSSLVLNVGALHGGFNLYNNGFSGGAVAA
                     VLVPVIEAIRTGRERNGS"
     gene            180..527
                     /locus_tag="P5219_05485"
     CDS             180..527
                     /locus_tag="P5219_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26242.1"
                     /translation="MVHEHERVVRLVSELQNFLFSMGAVEIRTEILETTDRFNVFIKG
                     NTRGNICTKLEKLRKLLKMPRQTSMEEELWLLAGSVDYDTELSLVGMMTDSAEVRCDG
                     EAGVVEIELMRKK"
     gene            938..1492
                     /gene="ilvN"
                     /locus_tag="P5219_05490"
     CDS             938..1492
                     /gene="ilvN"
                     /locus_tag="P5219_05490"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRX26243.1"
                     /translation="MRRTLSVISEDNPGVLTRLAGLIYRRGYNVESLSVGRTDIPGFS
                     RFTVVVNGDDGVIDQVTKQLDKLIEVVSVRRLVPGRSVERWLLLMKVKAPVHMRHQVL
                     QTAEIFRCRVVDVGAEAVVLELTGDKGKVEACVEALRPYGILEMTSSGSVAMERAGFA
                     EARDEGEEKAYSSADSFSSVAFTF"
     gene            1515..>2459
                     /gene="ilvC"
                     /locus_tag="P5219_05495"
     CDS             1515..>2459
                     /gene="ilvC"
                     /locus_tag="P5219_05495"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRX26244.1"
                     /translation="MAKVLYESDAKPEVLKNRKVAILGYGSQGHAHAQNLKESGVSVV
                     VGLHAGSRSKAVAEKDGFIVKSVPEAVAEADVVMFLMPDHVQAKVFETEVRPALKPGT
                     TLGFSHGFTIHYGQIVPPKTADVFMVAPKSPGHLVRRMYTEGKGVPGLLAVYQDATGM
                     AREIGMAYAAAIGCARAGVIETTFAEETETDLFGEQAVLCGGVTELIKAGFDTLVEAG
                     YQPEIAYFECLNELKLIVDMMFEGGMAWMRYSVSDTAKYGDCVAGPKVVDGHTREVMK
                     DLLKAVQDGTFAKDWILENQCGRPRMRAWQARENCHLIE"
BASE COUNT          527 a          653 c          811 g          468 t
ORIGIN      
        1 tgcgggagag ttcggggttt tatggggtgt cgtggccgga ttcctccatt cctccctggt
       61 cctgaacgtc ggtgcgctgc acgggggatt caacctatac aacaacggct tttcgggggg
      121 agcggtggct gccgttctcg ttcccgtcat cgaggccatc aggacaggga gggaacgtaa
      181 tggttcatga gcacgagcgg gtcgtccggc tggtttccga gctgcagaat ttcctctttt
      241 ccatgggggc ggtggaaatc cgcacggaaa tcctggaaac aaccgatcgc ttcaacgtct
      301 tcataaaggg aaacacccgg ggcaacatct gcacaaagct ggaaaagctc aggaaactcc
      361 tgaagatgcc ccggcagacc tccatggaag aggaactctg gctgctggcc ggttccgtcg
      421 actatgacac ggaactttcg ctggtgggga tgatgactga ctccgccgag gttcgctgcg
      481 acggggaggc tggcgtggtg gaaatcgaac ttatgagaaa aaagtgaaac tcggcctttt
      541 gtttaaaaac gatatagtta ttggaaatct ttgcttattt gaacttggaa aggggggact
      601 tgacaaggat ccttaagccc cttatgattc cccccaataa acattaaccg gcaggcgcaa
      661 gcccttgacc ggcatcctgt aaagaaggag ggataatccg tgaagacaag caatcctgtc
      721 agcctgcagc tcgtcctggt actggcgctg attattggac acgggttccc tcctggggta
      781 gccggtgcct aggcgccgcg tttaggcaaa cccaggccgg gacccgcaag aaaaagcggg
      841 tcccggcctt tttttatacg ttttgacgct tcatgaagag gaagacggga atatctcaag
      901 aggcagagag aaagctattc aaggaggtgc cagtgcgatg agaaggactt tgagcgtcat
      961 ttccgaagac aacccgggtg tcctgacacg gcttgcgggc ctgatctaca ggcgcggcta
     1021 caacgttgag agcctgagcg tagggcggac cgacattccg ggattttcac gattcacggt
     1081 ggtcgtgaac ggtgacgacg gagtgatcga ccaggtcacc aagcagctgg acaagctgat
     1141 cgaagtcgta tcggtccgca ggctggttcc ggggaggtcg gtggaacgct ggcttctgct
     1201 gatgaaagtg aaggcccccg tacacatgag gcaccaggtg ctccaaaccg cggagatttt
     1261 ccgctgccgg gtcgtggacg tgggcgcgga ggcggtcgtc ctggagctca cgggggacaa
     1321 gggaaaggtc gaggcctgcg tggaggccct tcgtccctac gggatcctcg agatgacctc
     1381 gagcggttcg gtggcgatgg aacgtgccgg tttcgccgag gcgcgggatg agggcgagga
     1441 aaaggcgtat tcttccgcgg acagtttttc gtccgtggct tttacattct gacagaaaag
     1501 ggagagggta gaaaatggca aaggttcttt acgaaagcga cgcgaaaccg gaagtcctga
     1561 aaaacaggaa ggtggccatc ctgggctacg gcagccaggg gcacgcccac gcccagaacc
     1621 tgaaggaaag cggagtctcc gtggtggttg gccttcatgc cgggagccgc tcgaaggcgg
     1681 ttgccgagaa agacggcttt atcgtaaaaa gcgttcccga ggcggtggcc gaggctgacg
     1741 tcgtcatgtt cctgatgccg gaccacgtcc aggcgaaggt tttcgagacc gaggtcaggc
     1801 cggcgctgaa gccggggacg acgctgggct tctcccacgg cttcacgatc cactacggcc
     1861 agatcgttcc cccgaagacc gccgacgttt tcatggtggc cccgaaaagc cccggccacc
     1921 tcgtgcgccg gatgtacacc gagggaaagg gcgttccggg tcttttggcc gtctaccagg
     1981 acgcaacggg catggctcgt gagatcggga tggcctacgc ggccgccatc ggctgcgcca
     2041 gggcgggggt catcgagacg accttcgcgg aggagacgga aaccgatctc ttcggggagc
     2101 aggccgtgct ttgcggtggc gtgaccgaac tcatcaaggc gggtttcgac accctggtcg
     2161 aggccggcta ccagcccgag atcgcctatt tcgagtgcct caacgaactc aagctcatcg
     2221 tggacatgat gttcgaggga ggcatggcct ggatgcgcta ctccgtcagc gacacggcca
     2281 agtacggcga ctgcgtggcg ggtccgaagg tcgtcgacgg gcacacccgc gaggtcatga
     2341 aggacctgct gaaggccgtc caggacggga ccttcgccaa ggactggatc ctcgagaacc
     2401 agtgcggacg gccgcgcatg cgggcctggc aggccaggga gaactgtcac ctcatcgag
//
LOCUS       DAOTHD010000269         2459 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_50675_length_2459_cov_2.267471, whole genome shotgun sequence.
ACCESSION   DAOTHD010000269 DAOTHD010000000
VERSION     DAOTHD010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50675_length_2459_cov_2.267471"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="P5219_05500"
     CDS             <1..1072
                     /locus_tag="P5219_05500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HRX26245.1"
                     /translation="EVRTDRIGNLFGILRGADGGLGNALMIGSHIDTVIHAGPYDGCY
                     GVLSGLAVLRAFREAGAVPPRPLVVAAFTNEEGVRFQPDMLGSLVAVGGMPLGEALDI
                     TDEKGRTVGEALAAIGCSGGEDPGFLLPAEYLELHVEQGPRLDAEKKRIGVVEGVQGI
                     SWWRITIEGKANHAGTTPTNLRHDAGYASALVSVFLRELSLSAGTTLATVGTIDFEPG
                     AVNVIPSKAVFTVDLRDPDGAKLTEAEKRLAAFLEKTALEEGVRISSEHLVRFEPVVF
                     DRELVQAVEETAASKGLSSMRLVSGAGHDAQMMARVCRTAMIFVPSAGGISHSPEEHT
                     SGEDLAAGVEVLLDVVLRVLGF"
     gene            complement(1105..2139)
                     /gene="ord"
                     /locus_tag="P5219_05505"
     CDS             complement(1105..2139)
                     /gene="ord"
                     /locus_tag="P5219_05505"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF040740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HRX26246.1"
                     /translation="MKPIRVVVWGLGTMGSLMAKILSEKEGIDITGAVDLNPETAGKS
                     LGAITGSSRHGEITVTADAGEALAQDADLVLLATSSFISETAEPVRQIVKSGKNVITI
                     AEEWAFPRRTAPKEAEEIDRLAKTFGVSVLGTGVNPGFVLDTLIIALSGTCADVSKIT
                     ARRVNDLSPFGPTVMASQGIGLSPEDFEKGLESGAIAGHIGFEQSIHMIASSLGIRLE
                     KITQSLEPIISRTSRKTPCVSISPGQVAGCNHRAEGWSDGRPVIILEHPQQVHPETEN
                     VETGDFIHIEGRPDIRLAIQPEIPGGTATAALAVNMIPHVLNAPAGLACMADLPVPSA
                     RLADFRQFLK"
BASE COUNT          462 a          684 c          789 g          524 t
ORIGIN      
        1 cgaggtccgg acggacagga tcggcaatct tttcggcata ctccgagggg cggacggcgg
       61 actcgggaac gcactgatga tcggttccca cattgacacg gtgatccatg cggggcccta
      121 tgacggctgt tacggcgtcc tgtccggcct ggcggtgctc agggcttttc gggaggccgg
      181 cgcggttccg ccccggccgc ttgtggtagc ggcgtttacg aacgaagagg gcgtccgctt
      241 ccagcctgac atgctgggct cccttgtggc cgtggggggc atgcctctcg gagaggcact
      301 ggacatcacc gacgaaaagg gtcgcaccgt gggcgaagcc cttgccgcca taggctgttc
      361 cggcggagaa gaccccgggt tcctgcttcc cgcagaatat ctcgagcttc acgtggagca
      421 gggcccccgg cttgacgccg aaaagaaaag gatcggtgtt gttgagggag tgcagggaat
      481 ttcctggtgg cggatcacca ttgagggaaa ggcgaatcac gccgggacga ccccgacaaa
      541 tctgcgtcat gacgctgggt atgcctccgc tctggtgtcg gtgttcctga gggagctttc
      601 cctctctgcg gggaccaccc ttgccaccgt cggcaccatc gacttcgaac ccggagcggt
      661 gaacgtcatc ccctcgaagg ccgttttcac tgtggacctg cgggatccgg acggtgcgaa
      721 gctgacggag gccgagaagc ggctcgcggc cttcctggaa aaaacagccc tcgaagaagg
      781 cgttcggatt tcgtcggagc accttgtgcg cttcgaaccc gtagttttcg acagggaact
      841 tgtccaggcc gtcgaagaga cggctgcttc gaaaggcctt tcctccatgc ggctggtctc
      901 gggagccggc cacgatgccc agatgatggc cagggtctgc agaacggcca tgatttttgt
      961 acccagcgcc gggggcatca gccacagtcc ggaggagcac acttccgggg aggatctggc
     1021 ggcaggggtt gaagttcttc ttgacgtggt tctgagagta ctcgggttct gagtgcaaac
     1081 agggcggggg tattccctcc cgccttattt cagaaactgc cggaaatcag caagcctcgc
     1141 tgagggaacc ggaaggtcgg ccatacaggc aagacccgcc ggggcattca gaacatgagg
     1201 gatcatgttg acggcaaggg ctgccgtggc agttccccca ggtatctcgg gctggattgc
     1261 gagccggatg tccggtctgc cttcaatgtg gatgaaatct ccggtctcga cgttttcggt
     1321 ttccgggtga acctgctggg gatgttcgag aatgatgacg ggcctgccgt ccgaccatcc
     1381 ctctgcacgg tgattgcagc cggcgacctg cccgggggag atgctcacgc aaggggtctt
     1441 tcgtgaagtg cgggatatga tgggttcaag ggactgggtg attttttcca gccggattcc
     1501 cagggaagag gcgatcatgt gtatcgattg ttcgaaaccg atgtggcctg ctatggcgcc
     1561 gctttcaagt cctttttcga agtcctccgg cgagaggccg attccctggc tggccattac
     1621 cgtcgggccg aagggtgaaa ggtcgttcac ccgcctggcg gttattttgc ttacgtccgc
     1681 acaggttccg gaaagggcta ttatcagcgt atcgagcacg aatcccggat tcacgcccgt
     1741 tcccaggacg gagacgccga aagttttcgc gagccggtcg atttcctctg cctctttcgg
     1801 ggctgtccgg cgggggaagg cccattcttc ggcaatggtg atcacgtttt tgccgctttt
     1861 cactatctgc ctgacaggtt ccgcggtttc actgataaac gaggacgtgg ccaggaggac
     1921 gaggtccgcg tcctgtgcca gcgcctcccc ggcatccgct gttaccgtaa tttcgccgtg
     1981 gcgggaggag cctgttatcg ctcccagaga tttccctgct gtttccgggt tcagatccac
     2041 ggcaccggtg atatcgattc cttccttttc agagaggatc ttcgccatga gcgagcccat
     2101 cgttccgagt ccccatacga ccactctgat cggtttcatt gcaggccacc tccgcccggg
     2161 gttatgatgc acgaaaagta tggtgtctcc cggacaccgt gtcaatgcaa gaatacgata
     2221 tgatagtggg gaactgactc agccgactgc ggaaaatgat cccgacatgc aggggagagg
     2281 ggagtggagg aatgatgaat atggttctgg acagcgtcct tcaggccatc ggcagcacgc
     2341 acatggtgag gcttgacagg ctggcctccg agtggggact gagcggcggg cttttcggca
     2401 agcttgaata tctgaacccc ggattcagca aaaaagaccg gatagccctc agaatgatt
//
LOCUS       DAOTHD010000270         2454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_50801_length_2454_cov_2.635682, whole genome shotgun sequence.
ACCESSION   DAOTHD010000270 DAOTHD010000000
VERSION     DAOTHD010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50801_length_2454_cov_2.635682"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(391..930)
                     /locus_tag="P5219_05510"
     CDS             complement(391..930)
                     /locus_tag="P5219_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRX26247.1"
                     /translation="MDFVDLVRKRYSLRKYSSFPVAEAVIDKCLEAARLAPSACNSQP
                     WTFLVANTPEKVASLAKAAFSGIYSMNSFAANAPVLITVLTERSGYAASMGGFFRGTQ
                     FSLMDVAIACEHLSLQAAELGLGTCMFGWLDEKAVKRELSLPKAAKIDLMMSLGYPPD
                     EDCLPPKKRKSLEEIRRYL"
     gene            complement(947..1651)
                     /locus_tag="P5219_05515"
     CDS             complement(947..1651)
                     /locus_tag="P5219_05515"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRX26248.1"
                     /translation="MGGLYDFLYGVFHGDGAAAYAALYAWGILGMTLSPCSIATIPLV
                     VGYVSDSDNPTFWSAFRISVVFSLGVFVNLAFLGGLLTSAGVFLGGIDRYTNYIVAGV
                     FVLFGLHLLGLINLPWFRGGSGPTTKHTGYRGALALGTLSGLALGPCSFAYSAPVIFL
                     AVQTAATDIVRALLLVAAYGAGHCTVIIAAGSATDAFSRLFSGAGGKTAAWLGKISGL
                     LLLVAAGYLIYNAPTI"
     gene            complement(1661..1897)
                     /locus_tag="P5219_05520"
     CDS             complement(1661..1897)
                     /locus_tag="P5219_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRX26249.1"
                     /translation="MSKVLDQFSEKHGDKFEVQIVNVREYPDVARLYQVRYVPTLVFL
                     DEAGKMLDKRVGYMPLDALEKHWEGLGYDMKEGN"
     gene            complement(1913..2035)
                     /locus_tag="P5219_05525"
     CDS             complement(1913..2035)
                     /locus_tag="P5219_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26250.1"
                     /translation="MTINRLFLIFAALAVVLLLPYSGYAADTARPKLVELMSPG"
     gene            2184..>2454
                     /locus_tag="P5219_05530"
     CDS             2184..>2454
                     /locus_tag="P5219_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRX26251.1"
                     /translation="MEKENDRINVLYEELVAVRRRLHACPELDFDLKETAGIVGGQLD
                     SLGIPWRFVAGSGIAALLEGKEPGRTVLLRADMDALPVEEEWEVPW"
BASE COUNT          491 a          671 c          828 g          464 t
ORIGIN      
        1 gggaaggcca cggggttgtc tttgtttgtc atcctgagga gcgggtctgg gcgacgaagg
       61 atccgctatt gatcccagga ttcaagaatc gagatccctc gcgatcgctc gggatgacaa
      121 accggggccg agatccctcg gccaaaaatc cggcctcggg atgacaaacc ggaagcgaga
      181 tccctcgcat tcgctcggga tgactacctg gccctgtcgt cccgagcgag cgaagcgacg
      241 aggggcctcg gggttgtctt tgtttgtcat cctgaggagc gggtctgggc gacgaaggat
      301 ctcgagtttg actctgaatg cagtaaaaag cgagatcctt cgctgcgctc aggatgagag
      361 gctattcgtg acgacaaggt cctttgaagg tcagagatac ctccggattt cctccaggga
      421 tttccgtttc ttcggaggga ggcagtcttc gtcgggcggg tagccgaggg acatcatgag
      481 gtcgatcttc gctgccttcg gcagcgatag ttcccgcttc acggccttct cgtccagcca
      541 gccgaacatg caggtcccca ggcccagctc ggcggcctgg agggacaggt gctcgcaggc
      601 gatggccacg tccatgaggc tgaactgggt gccccggaaa aagcccccca tggacgcggc
      661 gtaccctgac cgttcggtga ggacggtgat cagcaccggg gcattggccg cgaaggagtt
      721 catggagtaa atcccgctga aggccgcttt cgccagggaa gccacctttt ccggtgtgtt
      781 ggccaccagg aaggtccagg gctgggagtt gcaggccgac ggggcgagcc gggcggcttc
      841 gaggcatttg tcgatgaccg cttccgccac ggggaaggac gagtatttcc ggaggctgta
      901 ccgttttctc accaggtcga cgaaatccat ggcccgtcct cccgcctcag atggtggggg
      961 cgttgtagat caggtaccct gccgcaacga gaagaagaag gccgctgatc tttccgagcc
     1021 atgcggcggt ttttccgcct gcgcctgaga agaggcggga aaaggcgtcg gtggcgcttc
     1081 ctgcggcgat gatcaccgta cagtgaccgg ctccgtaggc cgccacgagc agcagggccc
     1141 ggacgatgtc ggtggcggcg gtctgtacgg cgaggaagat caccggggcc gagtaggcga
     1201 agctgcacgg tccgagggcc aggccggaga gggttcccag ggcgagggca ccccggtagc
     1261 ccgtatgctt cgtcgtcggg ccggatccgc ccctgaacca tggcagattg atcagtccga
     1321 ggaggtggag gccgaagagg acgaagactc ccgccacgat gtagttggtg tagcggtcaa
     1381 tgccgccgag gaagaccccg gcggaggtga gaagcccccc gaggaaggcg aggttgacga
     1441 agacgccgag agagaagacc acggaaatgc ggaaggccga ccagaacgtg gggttgtccg
     1501 agtcgctcac gtaccccacc accaggggta tggtggcgat gctgcaggga ctgagggtca
     1561 ttcccagtat tccccaggcg tacagggcgg cgtatgccgc cgccccgtcg ccgtggaaca
     1621 ctccgtagag aaaatcatac aatccgccca tgatactgtg tcagtttcct tccttcatgt
     1681 cgtagcccaa tccttcccag tgcttctcca gggcgtcgag gggcatgtag cccacccgct
     1741 tgtcgagcat ctttcccgcc tcgtcgagga agacgagggt ggggacgtac cgcacctggt
     1801 agagtctggc cacgtcgggg tattcccgga cgttcacaat ctgcacttcg aacttgtccc
     1861 cgtgcttctc cgaaaactgg tccaaaactt tggacatctc aacgcaggcc ggtcagcccg
     1921 gagacatgag ctccacgagt ttcgggcggg cggtatcggc cgcgtatccg gagtagggaa
     1981 gcaggagaac tacagcgaga gcggcaaata taaggaacaa tcggttgatg gtcatttttc
     2041 atcccccctg ggtgtatttc ggtatatgaa attgcgggat tattgtagct tccggaggag
     2101 gggacggtca acatggaaag ggctgatttt ccctttccgg cgaattttcg gtagaatggc
     2161 ggagcggaag ggaagtgaac ggagtggaaa aagagaacga tcggatcaat gtattgtatg
     2221 aagaacttgt cgccgtccgc cgcaggcttc acgcctgtcc ggaactggat ttcgacctga
     2281 aggagacggc ggggatcgtg ggcgggcagc tcgattccct tggcattccg tggcggttcg
     2341 tggcaggaag cggcattgca gctctcctcg aagggaaaga gcccgggcgg acggtgctgc
     2401 tccgggcgga catggatgcc ctgcccgtgg aggaggaatg ggaagttccc tggc
//
LOCUS       DAOTHD010000271         2452 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_50866_length_2452_cov_2.422194, whole genome shotgun sequence.
ACCESSION   DAOTHD010000271 DAOTHD010000000
VERSION     DAOTHD010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50866_length_2452_cov_2.422194"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="P5219_05535"
     CDS             complement(<1..1015)
                     /locus_tag="P5219_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HRX26252.1"
                     /translation="MKIETVKNSIGREVPVLVPGLGEFVPFAGPYARLGGDYAWTPRL
                     FPRKVAAPSTDKVAESLEEAIRRSGLESGMTISFHHHMRNGDSLVCMVLSLLEKMGIR
                     NITLAPSSLGDCHDCVADMVRKGVVTRLNTSGVRGEVGRAISGGRFEVPVMIRSHGGR
                     ARAIEEGSLHIHVAFLAAPACDRLGNFTGSSGKTACGSMGYAMVDARYADHVIAVTDN
                     LVEYPLAPRISVPQYLVDQVAVVDSIGDPSKIATGATRVTRDPVQLRIAELAFGLIRA
                     SGMLKNGCSFQTGGGGPSLAVARYVREYMKEKAVRGSYCIGGITGYMADMLREGLFTA
                     LFDV"
     gene            complement(1012..1607)
                     /locus_tag="P5219_05540"
                     /pseudo
     CDS             complement(1012..1607)
                     /locus_tag="P5219_05540"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     assembly_gap    1608..1617
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1618..1898)
                     /locus_tag="P5219_05545"
     CDS             complement(<1618..1898)
                     /locus_tag="P5219_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRX26253.1"
                     /translation="MNRIDGRPRRSLLYIPGNNPGMIQNCAIYGSDGVLLDLEDSISI
                     TEKDAARKLVKHALKTLDFGSVERVVRINGRDTPFFERDLGEIIPARPDA"
     gene            complement(1895..>2452)
                     /locus_tag="P5219_05550"
     CDS             complement(1895..>2452)
                     /locus_tag="P5219_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRX26254.1"
                     /translation="SCAAGAMVPLLTLGIPGSGTTAVMLGALMMLGVRPGPILFSQFP
                     EIAWGVIASILLGNIILVIINLPLAIPLVQLLKIPQRIMLPLILGMGFMGTYFLNYSS
                     FDFILVMAFALGGYIFSKLEIPLPPLVLALILGGTTEQSFRNSMSLSDGSLAIFFQKP
                     ISVTLIVLAAVSLVYSLLKRRKYAS"
BASE COUNT          428 a          756 c          824 g          434 t
ORIGIN      
        1 gcacgtcgaa gagggcggtg aagagccctt ccctgagcat gtcggccatg tagcccgtga
       61 tgcccccgat gcagtagctt ccccggacgg ccttctcctt catatattcc ctcacgtacc
      121 gtgccacggc gaggctcggt ccgccgcctc ccgtctggaa ggaacagccg tttttcagca
      181 tgcccgacgc ccggatgagg ccgaaagcca gctctgcgat acggagctgc acggggtcgc
      241 gggtgacccg ggtggcaccg gtggctattt tcgacgggtc gccgatggag tccaccacgg
      301 ccacctggtc caccaggtac tggggcacgg agatcctcgg tgcgaggggg tactccacga
      361 ggttgtccgt gacggcgatg acgtggtcgg cgtaccgggc gtccaccatg gcgtagccca
      421 tggagccgca ggcggtcttt cccgaggagc cggtgaagtt gccgagcctg tcgcacgccg
      481 gtgcggcgag aaaagccaca tgaatgtgga ggctgccttc ttcgatggcc cgggcccgtc
      541 ctccgtggct ccggatcatc acgggcactt cgaacctgcc gccggagatg gccctgccca
      601 cctcgccccg gacgcccgag gtgttcagcc gggtgaccac gcctttccgg accatatcgg
      661 ccacgcagtc gtggcagtct cccagggaac tgggggcgag ggtgatgttc cggatgccca
      721 ttttttccag cagggagaga accatgcaga ccagggaatc cccgttccgc atgtgatgat
      781 ggaaggagat ggtcatcccg ctctccagtc ccgagcggcg gatggcctcc tccaggcttt
      841 cggcgacctt gtccgtggac ggggcggcca ccttcctggg gaagagccgg ggtgtccatg
      901 cgtagtcccc gcccagccgg gcataggggc ccgcgaaggg gacgaattct ccgaggccgg
      961 gaacgaggac cggtacctcc ctgccgatgc tgttcttcac agtttcgatc ttcattcagc
     1021 ctccacctcc attcccgcga ggcgggcgag ttccagggtt ctttgcgcct gggccaccac
     1081 gggggcgtcc accatgcggc cgtccacggc caccacgccc agtccccgtt cctccgcctc
     1141 ccgggctccg cggacgatcc gctccgcctt ccgcacgtcc ttttcttcgg ggcggaatgc
     1201 ctcgtggatc acggcgatct gggacgggtg gatggcggcc ttgccggaaa aaccgaggcc
     1261 gacgctcatg gccgcctgtt cacggagtcc ctccaggtcg tcgatgtcgg tgtagaccgt
     1321 gtcaaaggcg agaaggccgg cagccttcgc cgccaggacc acggcactcc gggcatagag
     1381 catctcaagg ccttcccggg taattttgat gccgaggtcg gccgccaggt cctggccgcc
     1441 gatggtgagc cccaggatgc ggggggacga cattgctatg gcggacgcgt tcacgatggc
     1501 ccgggcggtc tcgagcatgg catggatccg tacggagccc ttttccatgc cgttttcctc
     1561 ctcaaggcgg gtgatgatgt cgtccgccgc cctgacgtcg tcgggggnnn nnnnnnngca
     1621 tccggcctcg ccgggataat ttctccgaga tcccgttcaa agaagggcgt gtcccggccg
     1681 ttaatccgca ccacccgctc cacggagccg aaatccagag tcttgagggc atgcttcacc
     1741 agtttccggg cggcatcctt ctccgttatg gagatggagt cctccaggtc gaggaggact
     1801 ccatccgagc cgtagatggc gcagttctgg atcatgcccg ggttgttccc cgggatgtac
     1861 agcagtgacc gccggggtcg gccgtcaatc cggttcatga tgcgtacttc ctcctcttca
     1921 ggagcgaata gaccagggag acggcggcga ggacgatgag ggtcacggag atgggcttct
     1981 ggaagaaaat ggccagcgac ccgtcggaga gggacatgga attcctgaag gactgttccg
     2041 tggtgcctcc gaggatcagg gccagcacca ggggcggcag gggaatctcc agcttggaga
     2101 agatgtaccc cccgagggcg aaggccatga caaggatgaa atcgaaggag ctgtagttca
     2161 ggaaataggt tcccatgaaa cccatgccga ggatcagggg aagcatgatc ctctggggga
     2221 tcttgaggag ctgcaccagc gggatggcca gggggaggtt gatgatcacc aggatgatgt
     2281 ttcccaggag gatggacgct atgacgcccc atgcgatctc gggaaactgg gagaagagga
     2341 tggggcccgg ccggacgccg agcatcatga gggcgccgag catgaccgcc gtggtgccgg
     2401 accccggaat gccgagggtc agcaggggga ccatggcgcc ggcggcgcag ga
//
LOCUS       DAOTHD010000272         2446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_51023_length_2446_cov_3.267252, whole genome shotgun sequence.
ACCESSION   DAOTHD010000272 DAOTHD010000000
VERSION     DAOTHD010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51023_length_2446_cov_3.267252"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..933
                     /locus_tag="P5219_05555"
     CDS             175..933
                     /locus_tag="P5219_05555"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HRX26255.1"
                     /translation="MTMGLYEKIQDRLLSAGEGKVESLVLGWKGSAVVLKDGRWGLGA
                     IPPFGGASLSPREDHTRRLLASGARDLARLLVSPFPQEYAAASAAAAALAPATGEGLP
                     LESLLPLPGGDRVSLVTPDPWVTDFLRDWNWNVSVFDDGRRGLNVFPEWTASQHLNSS
                     GWVWLTAEVFRTRAFFSLLPRPAEKTVVLQGPGIPFLPDVYRRAGISYLVLPQAPGTD
                     GAAAIRYVASGGTPWTCPDLLWRVYPLQDRVKAP"
     gene            930..1718
                     /locus_tag="P5219_05560"
     CDS             930..1718
                     /locus_tag="P5219_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HRX26256.1"
                     /translation="MIRILSTAAALLSAAILSLAGLMGGPDAHELLSSAGIEVHDGDT
                     ISVPLNGERTTIRYLLIDTPELHHPRRGEEELALEAAGANRDLISSGPLRLEFDEELR
                     DRYERLLAYVWVALPEGELLVNEELVRRGFALPLIIPPNGKHAPRIFSAMEEAARSGA
                     GLWSRSENRIFNPSQVWSEATVLAGSFVTVRMTLERVEERGRRILLSQGRLTVTAYRN
                     SRTEALLGLRKGDRVTVRGKLVLSPRGCEIPVASFLQVSRDSDG"
     gene            2067..>2446
                     /locus_tag="P5219_05565"
     CDS             2067..>2446
                     /locus_tag="P5219_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26257.1"
                     /translation="MSKPGKKAVLFFFLLAVPAAAVLFLISGKDAGTDLFKTSLNDSL
                     RDLPGWSLEAGRFSGNPVTGFTAWDVRLSFENDEILRADSLTVSLSLLSLLRGSSGID
                     RISLKNGFVAAEPLFAAAGKTELPS"
BASE COUNT          420 a          788 c          719 g          519 t
ORIGIN      
        1 ccccgtaccg tttcaggatt tcgccccggg aggagaggat ctgttcatcc acgtggtcca
       61 ccggcgtgtc ccctttcgtt ccagagttca tacggggaac attatacccc tttcttcccg
      121 gggtgcgccc tttccccgga catccgggga gtatagaatg gagggaaagg gggtatgacc
      181 atgggcctgt acgagaaaat ccaggaccgg ctcctttcag ccggggaagg gaaagtggaa
      241 tcccttgtcc ttggctggaa gggaagcgcc gtggtcctga aagacgggcg ctgggggctc
      301 ggagcgatac cgcccttcgg cggcgcgtcc ctttctcccc gggaggacca tacgaggcgc
      361 cttcttgcct cgggagcccg ggacctggcc aggcttctcg tctcgccttt cccccaggaa
      421 tatgccgccg cttcagcggc cgctgccgcc ctcgcccctg cgacggggga gggccttccc
      481 ctggaaagcc tcctgcccct tcccggaggg gacagggtgt ctcttgtgac ccccgatccc
      541 tgggtcaccg attttctccg ggactggaac tggaacgttt ccgtcttcga cgacggcagg
      601 cggggtctga acgttttccc tgaatggacg gcctcccagc acctgaactc ctcgggctgg
      661 gtgtggctca ccgccgaggt cttcagaacg cgggcctttt tctccctcct tccccggccg
      721 gcggagaaaa cggtagtgct ccagggaccc ggaattccct ttctgcccga cgtctaccgg
      781 agggcgggca tttcctatct cgtcctcccg caggctccgg gaacggatgg agctgcggca
      841 atccggtacg tggcgtccgg gggcacgccg tggacgtgcc ctgaccttct ctggcgggta
      901 tatcccctgc aggaccgggt gaaggcgcca tgatccgcat cctctccacg gctgcggccc
      961 tcctttcggc ggcaatactc tcccttgccg ggcttatggg cggtccggac gcccatgagc
     1021 ttctctcctc ggcgggcatc gaggttcacg acggagacac catttccgtt cccctgaacg
     1081 gagagcggac caccatccgc tatcttctca tcgacacccc ggaactccac catccccgcc
     1141 ggggagagga agaactggcc cttgaagcgg ccggggcaaa ccgggacctc atttcttccg
     1201 gccccctccg cctggaattc gacgaggagc tcagggaccg ctacgaaagg ctccttgcct
     1261 atgtctgggt ggctcttccg gaaggagagc tgctggtgaa cgaggagctg gtgcgacgag
     1321 gatttgccct gccgctgatc attcccccca acggaaagca cgcccccaga attttttccg
     1381 ccatggagga ggccgccaga agcggcgcgg ggctctggag ccgttcggaa aacaggattt
     1441 tcaacccttc ccaggtgtgg agcgaggcga cggtccttgc cggcagcttc gtcacggtac
     1501 ggatgaccct ggagagggtg gaggagcggg ggcggaggat actcctttcc cagggaaggc
     1561 ttacggtcac cgcctacagg aattcccgca cggaggccct tctgggcctc aggaagggcg
     1621 atcgggtgac tgtccggggg aagcttgttc tttctccccg gggatgcgaa attcccgtgg
     1681 cctcctttct ccaggtcagc cgggattccg acgggtgaaa cacagacggg gcctgccgct
     1741 ttttgttccc cgctttccta gtgtccccga aaaaatgtcc acaggccgcc gggttatcca
     1801 caaatcccct tttttcaacc cttttttgcc gtctttttgg atgttatcca ccaacttctc
     1861 cacatatcca catttccaca cctctccacg ggccgccata tgtaggttga acagatatga
     1921 ctattcgctc tttaatatat cagtaatcct tctctggtgt tcccggcggt cctcccctat
     1981 gattgcaccg tcgccgcccc ggtatataat gtctgccgaa gggcagcgga gctgaaaaca
     2041 gcactagccg aaaggaaacg taggacatga gcaaaccagg aaaaaaggcc gttctttttt
     2101 tctttctcct cgccgttccg gcggcggccg tcctgttcct catctcgggg aaagacgcgg
     2161 gaacggatct ctttaaaacg tctctcaacg actccctcag ggatctgccg ggctggagcc
     2221 tcgaggccgg gcggttttcc ggcaatccgg tcaccggctt cacggcctgg gatgtccgcc
     2281 tttccttcga gaacgatgaa attcttcggg cggacagtct caccgtctcc ctctcgctgc
     2341 tctcgctgct gaggggatct tcgggaatcg acaggatttc gctgaagaac ggcttcgtgg
     2401 cagcggaacc cctcttcgcc gccgccggca aaaccgagct cccgtc
//
LOCUS       DAOTHD010000273         2445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_51052_length_2445_cov_3.557322, whole genome shotgun sequence.
ACCESSION   DAOTHD010000273 DAOTHD010000000
VERSION     DAOTHD010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51052_length_2445_cov_3.557322"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1537)
                     /locus_tag="P5219_05570"
     CDS             complement(89..1537)
                     /locus_tag="P5219_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRX26258.1"
                     /translation="MDKRNMYGIIEDKRDFFVAVDRKVWEYAETAFVEFKSADALCEA
                     LEAEGFAVERPVAGLETAFCGSWGQGSPVIAFLGEYDALSGLSQRDGATEKDPLVPGG
                     NGHGCGHNNLGAGALAAAVAFRDYLKASGKSGTVRYYGCPGEEGGSGKAFMARAGVFD
                     DVDAALTWHPGSVNAVFPFSSLANFQVAYRFRGVSAHAAACPHLGRSALDAVELMNIG
                     VNYLREHVIPEARMHYAITDSGGFSPNVVQPKAEVLYLLRAPRTPQVQEIYERVNDIA
                     RGAALMTGTELETVFIKACANVVQNNVLEEVLYRNFTELGVPAYSEEDREFARKIFEG
                     IAPSERSQDLDQYSAPGGKAGKEAARRLRERPLADEVLPYGQSEFVLPGSTDVGDVSW
                     NVPTGQVWVGTWPNHTPGHSWQITSAGGVSLSHKGMLHAGKVLAAAAADLLDDPSLIE
                     RAKEELRLRLDGDSYRCAIPDNVHPKPLNSGR"
     gene            complement(1559..1687)
                     /locus_tag="P5219_05575"
     CDS             complement(1559..1687)
                     /locus_tag="P5219_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26259.1"
                     /translation="MDKTDCEMFAENLFFLKSGPAGCVPCPWEDCGISGIAGAEGH"
     gene            1745..>2445
                     /locus_tag="P5219_05580"
     CDS             1745..>2445
                     /locus_tag="P5219_05580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRX26260.1"
                     /translation="MFDVKKKAQELNDQVIRWRRTLHANPEVGFNTAATEKLIVEELE
                     KMGADEIRHGNGMRGIVALIKGKKPGKVLGIRADYDALNMTEDTGLPFAASNGNMHAC
                     GHDAHAAMLLGTAKILTECRDDLQGTVKLIFQPSEEDGKGAPAMIEDGLFENPAMDGI
                     IGLHIGSLWKGFKAGEAGYRFGALMAASDWFTVTFEGKGGHGATPHLTIDPVAMACQA
                     VNLLHQDMHVMIPLFL"
BASE COUNT          472 a          805 c          736 g          432 t
ORIGIN      
        1 caccacgccc cggacatagt ccgggaagac agagaacact tcgtccgcta tggtgtaaaa
       61 aatctccttc ataaaaaagc ctccgtccct atctgcctga attgagaggc ttgggatgga
      121 cattgtcggg gatggcgcag cggtagctgt cgccgtccag tcggaggcgg agctcctcct
      181 tcgcccgttc gatcagggac gggtcgtcca ggaggtcggc ggcggcagcg gcaagcacct
      241 tgcccgcgtg gagcatcccc ttgtgggaga gggacactcc tcccgccgag gtgatctgcc
      301 aggaatgacc gggagtgtgg ttcggccagg tgcccaccca gacctgcccc gtgggcacat
      361 tccagctcac gtcccccacg tcggttgagc cgggcaggac gaactcgctc tgcccgtagg
      421 gaaggacctc gtcagcgaga ggacgctccc ggagccgccg ggcggcttcc tttcccgcct
      481 ttccaccggg ggcggaatac tggtcgaggt cctggctccg ctccgacggg gcgatccctt
      541 cgaagatctt ccgggcgaac tcccggtcct cctccgaata ggcagggact ccgagttcag
      601 tgaaattcct gtagaggact tcctcgagga cattgttctg gaccacgttg gcgcaggcct
      661 tgatgaagac ggtctccagc tccgttcccg tcatgagggc ggcgcccctg gcgatgtcgt
      721 tcacccgttc gtagatttcc tgtacctggg gcgtccgggg agcccggagg aggtagagca
      781 cctccgcctt cggctgcacc acgttgggtg aaaacccgcc ggagtcggtg atggcgtagt
      841 gcatccgggc ctcggggatc acatgctccc ggaggtagtt caccccgatg ttcatcagct
      901 ccacggcatc cagggcgctc cgtccgagat ggggacaggc cgccgcgtgg gcgctcactc
      961 cccggaagcg gtaggccacc tggaagttcg ccagggaact gaaggggaag acggcgttca
     1021 cggacccggg gtgccacgtc agggcggcgt ccacgtcatc gaagaccccc gccctggcca
     1081 tgaaggcctt tcccgagccg ccctcctcgc cgggacagcc gtaatagcgg accgtgccgc
     1141 tctttcccga ggccttcagg taatcccgga aggccaccgc cgccgcgagg gccccggcgc
     1201 cgagattgtt gtggccgcat ccgtggccgt tgccgcccgg aacgagggga tccttttccg
     1261 tggccccgtc cctctggctg agcccggaca gggcatcgta ctcgccgagg aaggcgatca
     1321 cggggcttcc ctgcccccag gaaccgcaga aggccgtttc cagccccgcc acgggacgct
     1381 ccacggcaaa gccttccgcc tccagggctt cgcacagggc gtccgccgac ttgaactcca
     1441 cgaaggcagt ttcggcgtat tcccatacct tcctgtccac ggcgacgaaa aaatcccgtt
     1501 tgtcttcgat gatgccgtac atatttctct tgtccatgat tcctggcttt ctctcctttc
     1561 aatgcccttc agccccggca atgccggaaa tcccgcagtc ttcccagggg cagggcacac
     1621 agcctgcggg accgctcttc agaaaaaaca ggttttccgc gaacatttcg caatcggttt
     1681 tatccatgat atcatccttc gtaccatggt tctaatcggc aagggacaaa aggagatggt
     1741 atccatgttc gacgtaaaga aaaaagcgca ggagctgaac gaccaggtca tccggtggag
     1801 gcgaacactc cacgccaatc cggaagtcgg cttcaacacc gccgccacgg aaaagctcat
     1861 cgtcgaggaa ctcgaaaaaa tgggcgcgga cgagatccgc cacgggaacg gcatgcgggg
     1921 aatcgtggcc ctcatcaagg ggaaaaaacc cggaaaggtc ctcggcatcc gggcggacta
     1981 cgacgccctc aacatgaccg aggacacggg acttcccttc gccgcctcca acggcaacat
     2041 gcacgcctgc ggccatgacg cccacgccgc catgctcctg ggtacggcga aaatcctcac
     2101 ggagtgccgg gacgacctcc agggaacggt gaagctcatc ttccagccct ctgaggagga
     2161 cggaaaggga gcccccgcca tgatcgagga cgggctgttc gaaaaccccg ccatggacgg
     2221 catcatcggc ctccacatcg gaagcctctg gaagggcttc aaggccggtg aggccggcta
     2281 ccgcttcggc gccctcatgg cggcctccga ctggttcacc gtcaccttcg aaggaaaggg
     2341 tgggcacggg gccacacccc acctgacaat cgacccggta gccatggcct gccaggcggt
     2401 caacctgctc catcaagata tgcatgtaat gattccgctt ttcct
//
LOCUS       DAOTHD010000274         2438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_51263_length_2438_cov_2.438103, whole genome shotgun sequence.
ACCESSION   DAOTHD010000274 DAOTHD010000000
VERSION     DAOTHD010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51263_length_2438_cov_2.438103"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2438
                     /locus_tag="P5219_05585"
     CDS             <1..>2438
                     /locus_tag="P5219_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HRX26261.1"
                     /translation="LRRDLAEKVREEEATAGRLEITAKSLNWLTALEGLEKEISGLRE
                     EETELQGEWTAFEPHLERLHRALRAASLEGAHATLEALRKQQSDDSEALKTEQESLPE
                     LESRAREGAQALLEAELVTIRAKEELNRAAPLIAKVRSLDQRLADRRNALSDLDEECR
                     QDGGSLEKARESRDEKSELLAGEADRLKIAERWLEEHRQDEWLAGGLAGVEEQVNALV
                     FRQKDMADGETALERAADTLRQAKTKLEECRERTGAGRGDLEAVSEELRREQEALAHL
                     LDGRLLREYRAEKEALLREMAFLARIAELEDYRARLEDGKACPLCGSLEHPFAERNVP
                     LPDETEGKISILEKLIGEAEEREAAVLRLEKAEAAARQLVTDGEKDEGAALSAKTAAE
                     KALALSAEGLEKLRGDCLRLQSLLENRLQPLGISEIPEGDLLSLAGSLRERLKSWQEK
                     VREKTEIEKKIGLLESDLKWLDGTLETLTRALDEKKKRLETLLNEYDRLLDERKELYG
                     EKDPDGEEHRLKDALEAAGEAEKKARDLRDGLKERADGAKSRVESLNRIILRRQPEVN
                     RAEAAFSAGLSAAGFEGEEEFLEARLEAEQRNALSAGKKDLEDRRTALAARRTDRERK
                     LAEERARQVTDEPLEVLENRFRECEDALKTLREEVSGLRHRLNENAAARERVKEKKAA
                     LEAQGRECRRWDSLHELIGSADGKKYRNFAQGLTFDMMIGQANRQLQKMSDRYLLVRD
                     REQPLELNVVDGYQAGEIRSTKNLSGGESFIVSLALALGLSSMASRNVRVDSLFLDEG
                     FGTLDEEALDTALE"
BASE COUNT          563 a          650 c          875 g          350 t
ORIGIN      
        1 cctccggcgg gacctggcgg agaaggtgcg ggaggaagag gcgaccgccg ggaggctgga
       61 gataacggca aagtctttga actggcttac ggccctggag gggctggaaa aggagatctc
      121 cggcctgagg gaggaagaga ctgagctgca gggtgaatgg acagcctttg aaccgcacct
      181 ggaaaggctc caccgggctc tgagggctgc gtctcttgaa ggtgcccatg ccaccctcga
      241 ggccttgcgg aagcagcagt ccgacgattc tgaagccctg aagacagagc aggaaagtct
      301 ccccgaactg gaatcccggg cccgggaggg agcccaggct ctgctcgaag cggagctggt
      361 gaccatccgg gcgaaggaag aactgaaccg tgccgcgccc ctgatcgcga aggtccggtc
      421 cctcgaccag cggcttgcgg accgccggaa cgccctttcg gacctggatg aggaatgcag
      481 gcaggacggc ggaagcctgg aaaaagcccg ggaaagccgg gatgaaaaat cggagcttct
      541 ggccggtgag gcggaccggc tgaaaatcgc agaacgctgg cttgaggagc accggcagga
      601 tgaatggctg gcgggcggcc tggctggagt tgaggaacag gtgaacgccc tggttttccg
      661 ccaaaaggac atggccgatg gagagaccgc cctggagagg gcggcggaca cccttcggca
      721 ggcgaaaacc aaactcgaag agtgccggga gcgtaccggt gccggcagag gggaccttga
      781 ggcagtatcg gaagaactga gacgggagca ggaagcgctt gctcacctgc tggacggccg
      841 cttgctccgg gaataccggg cggaaaaaga agccctgctc cgggagatgg ccttcctggc
      901 caggattgcc gagctggagg actaccgggc gagactcgag gacggcaagg cgtgccctct
      961 ctgcggttcg ctggaacacc cttttgcgga gaggaacgtt cctttgcccg acgagacgga
     1021 ggggaagatc tccatccttg aaaaactcat cggggaggcg gaggagcggg aagccgccgt
     1081 gctgaggctg gagaaggccg aggcggcagc ccgtcagctg gtgacggacg gggaaaagga
     1141 cgaaggggcg gccctttcag ccaaaacggc ggcggaaaag gcattggctc tctcagctga
     1201 agggctggaa aaactgcggg gggactgcct gcgtcttcaa agtcttcttg agaacaggct
     1261 gcagcccctc ggcatttcgg aaattcctga gggggatctg ctgtccctgg cgggctccct
     1321 gcgggaacgg ctgaagtcgt ggcaggagaa ggtccgggag aagacggaga tcgaaaagaa
     1381 aatcggcctt ctggaaagcg acctgaaatg gctggacggc accctggaaa ccctgacccg
     1441 cgcccttgac gagaaaaaaa agcgcctcga aactcttctg aatgagtatg accgccttct
     1501 ggatgagcgg aaggaactct acggcgaaaa ggatcccgac ggggaggagc accgtctgaa
     1561 ggatgccctg gaagccgccg gggaggccga aaagaaggct cgggacctcc gggacgggct
     1621 gaaagaacgg gcagacgggg cgaaatcccg ggtggaatcc ctgaacagga tcattcttcg
     1681 gaggcagccg gaagtgaaca gggcggaggc tgccttttcg gccggtcttt ctgcggcagg
     1741 gtttgaaggt gaagaggaat tccttgaagc ccggcttgaa gcggagcagc ggaacgccct
     1801 ttctgcgggg aagaaggatc tggaagaccg ccggactgcg ctggccgcaa gacggacgga
     1861 ccgggaaaga aagctggcag aggaaagggc ccggcaggtg acggacgaac ccctcgaggt
     1921 actggaaaac cgtttccggg aatgcgagga cgccctgaag accttgcggg aggaagtttc
     1981 gggcctcagg cacaggctga acgagaatgc cgccgcccga gagcgggtga aggaaaaaaa
     2041 ggcggccttg gaggcccagg gcagggagtg ccgccggtgg gacagcctgc atgagctgat
     2101 cggctccgcc gacggaaaga aataccgcaa tttcgcccag ggactgacct tcgacatgat
     2161 gatcggacag gccaaccggc agctacagaa gatgagcgac cggtacctgc tggtccggga
     2221 cagggaacag cccctggagc tgaacgtagt ggacggctac caggccggag aaatccggtc
     2281 aaccaagaac ctctccggcg gagagagctt tatcgtcagt ctcgcgctgg ccctggggct
     2341 ctcctccatg gcgagcagga acgtccgggt ggactccctc tttttggatg aaggcttcgg
     2401 caccctggac gaggaagccc tcgacaccgc ccttgaaa
//
LOCUS       DAOTHD010000275         2437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_51298_length_2437_cov_2.188917, whole genome shotgun sequence.
ACCESSION   DAOTHD010000275 DAOTHD010000000
VERSION     DAOTHD010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51298_length_2437_cov_2.188917"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="P5219_05590"
     CDS             complement(<1..1251)
                     /locus_tag="P5219_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRX26262.1"
                     /translation="MTSGRLPLAVLLCFTLLFALPARGLGDPPRIVGSERGRTYHVFC
                     LDSGAPYIFRNGNGEITGMYADIFSALGNREGLKFSLSFGDEKEARRNIHLGKIDAVA
                     GSLYPTRPDSELFGYLPVKNLDLDNRNLPTELELLTHRLSGKELLSYVFTLPFLKEPA
                     TVFFPGPEAAGTDTLRGKTVLVSAESQEERYLAETLHFFSLRREDSRRSALSALVEGK
                     GQGAFLGAYQGIRLAGEMNREREISPLRPFPYILTKGIAVLKGQSSLAVQLLHGLETM
                     EKTGELDKIRELWVSEFEPSVFSLRQIWQAAGIAVILLAAFLAWNMMLKRKVALMVKE
                     REKILDFIRDGILAVDRDGKITMINRAARTLLGLSPEVVGKEADLCVPGLSAAMILSG
                     GRPVYNHQQNLNGALLSCSKVPVIQ"
     gene            complement(1238..>2437)
                     /locus_tag="P5219_05595"
     CDS             complement(1238..>2437)
                     /locus_tag="P5219_05595"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HRX26263.1"
                     /translation="ILTVEQLMIIALQRCRTARDAILLIGNLAERYGFLGSCANDGES
                     LSITDPSEAWIMEILGAGFDWQPGSRPGAVWVARRVPDNHAAVLCNVSRITFVDEESA
                     DFLFSAGYKEPAIRHGWYDPASGDPFNWRKAYAPDNGPWSPSSMWVRGRLHYIHKRLL
                     PSKQWDPYADTDSYPFSFKPEKPLSIREIMDIFRSGLEGTPFSMEDNPAWLVPGENRN
                     LLKSPKATPFPDRATRELLNIPYMRPIAAKTSYSFITQSRSWLPSAIGGVLWFSPDSP
                     HFSTYAPIYAGAHSIPESWSNFDKTRFSLDSARWAVLLAASLTNGNYQRAIQSLRSIR
                     DPLEKKTLDDFQGWDREAARVFLEKGALPDGWIDERVSGKLMEIHRTYEEILSLLIRE
                     GTIIDLW"
BASE COUNT          519 a          638 c          738 g          542 t
ORIGIN      
        1 ctggatgacc gggaccttgc tgcaggaaag aagggcgccg ttcaggttct gctggtggtt
       61 gtacaccggg cgtccccccg aaagaatcat cgcggctgac aggccgggaa cgcacaggtc
      121 cgcctctttt cccaccacct ccggggagag gccgagcaga gtacgcgcag cccggttgat
      181 catggttatc tttccatccc tgtccacggc gagaatgccg tcccgtatga aatcaaggat
      241 cttttccctt tctttcacca tcagggcaac tttgcgcttc agcatcatgt tccaggcgag
      301 gaacgcggcg agcagaatga cggcaatgcc tgccgcttgc catatctgcc ggaggctgaa
      361 gacggaaggc tcgaattccg aaacccacag ttcccggatt ttgtcgagtt cccctgtttt
      421 ttccatggtc tccaggccgt gaagaagctg cactgcgagg gatgactggc ctttgagaac
      481 ggcaattcct ttcgtaagga tgtagggaaa ggggcgaaga ggggaaatct ctctttccct
      541 gttcatttcg ccggcgagcc tgattccctg gtatgcccca agaaaggcgc cctgtccctt
      601 tccctcgacc agagcggaga gagcggaccg gcgggagtcc tccctgcgaa gggagaagaa
      661 atggagagtt tctgcaagat agcgttcttc ctgggattcg gcgctgacga gaaccgtttt
      721 gcccctcagg gtgtccgttc ctgcggcttc cgggccgggg aaaaataccg tcgccggttc
      781 tttcagaaaa ggcagggtaa aaacgtagga aagaagctct tttccggaaa ggcgatgggt
      841 gagaagttcc agctctgttg ggaggttcct gttgtcgagg tccaggtttt tgacgggcag
      901 gtaaccgaag agttcggaat caggcctggt tggatagagt gatcctgcca cggcgtctat
      961 tttcccgaga tggatgtttc tgcgggcttc cttttcgtct ccgaaggaaa gggagaactt
     1021 gagtccctcc ctgttcccga gggcagagaa gatgtcggcg tacattcccg tgatttctcc
     1081 gttcccgttc cggaaaatat agggagcccc ggaatcgagg cagaaaacat ggtaggttcg
     1141 tcccctttca ctgcctacaa tacggggcgg gtctcccagt cccctggccg gtaaagcgaa
     1201 gagcagggta aaacagagaa ggacggcaag aggaagccta ccagaggtca atgatggtcc
     1261 cctcccggat taacaggctg agaatctctt cgtaagtccg gtgtatttcc atcagcttcc
     1321 ccgacactct ttcatcgatc cagccgtcgg gaagcgcgcc tttttccagg aaaacacggg
     1381 cggcttccct gtcccatccc tgaaaatcat caagagtttt tttctcgagg ggatctctga
     1441 tggatcgcag agactggatg gctctctggt agtttccgtt tgtcagtgac gccgccagga
     1501 ggaccgccca tctggccgag tcgagagaaa aacgggtttt gtcgaagttg gaccagctct
     1561 ccgggataga gtgcgcaccc gcatagatag gagcgtaggt gctgaagtgg ggagaatcgg
     1621 gagagaacca cagaacccct ccgatggctg agggaagcca tgatctggac tgggtgatga
     1681 agctgtagga tgttttcgcc gcaatggggc gcatgtaagg gatattcaga agctccctgg
     1741 tggcacggtc cggaaaggga gtggctttgg gactcttcag caaatttctg ttttctcccg
     1801 gaacaagcca ggcagggttg tcttccatgc tgaagggagt tccctccagt ccggagcgga
     1861 agatatccat gatttccctt atggagagcg gtttttccgg tttgaaagag aaggggtacg
     1921 agtctgtgtc tgcataggga tcccattgct tcgagggcag gagccgcttg tgaatgtagt
     1981 ggagccttcc cctgacccac atggaggaag gagaccaggg gccgttgtcc ggtgcatagg
     2041 cctttctcca gttgaaagga tctcccgaag caggatcata ccatccgtgg cgtattgccg
     2101 gctccttgta gccggcggag aagagaaagt ctgcactttc ttcgtcgacg aaggtgatgc
     2161 ggcttacgtt gcacagaaca gccgcatggt tgtcggggac tctgcgggcc acccagacgg
     2221 caccggggcg ggagcccggc tgccagtcga atcctgcccc gagaatctcc atgatccagg
     2281 cttccgacgg atcggttatg gaaagggatt caccgtcgtt ggcgcaggat ccgagaaagc
     2341 cgtagcgctc cgccaggttt cctatcaaaa gaattgcgtc cctggccgtt ctgcagcgct
     2401 gcagtgcaat gatcatgagc tgctccaccg tcagaat
//
LOCUS       DAOTHD010000276         2432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_51436_length_2432_cov_2.958351, whole genome shotgun sequence.
ACCESSION   DAOTHD010000276 DAOTHD010000000
VERSION     DAOTHD010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51436_length_2432_cov_2.958351"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="P5219_05600"
     CDS             <1..502
                     /locus_tag="P5219_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRX26264.1"
                     /translation="DLDLILEAAIWAPSGHNAQPWHFLVIQDKGKIDEMSTKTISIMA
                     DSPIEWVRKLAGREGYHLFHRAPTVIVVSGKRPGSDLLFPHADCSAAIQNMLLAAESL
                     NIGSCWVGLTGFLFGIPEEVEALGIPEDCQPLYSVCFGYKDDSFVPPVPPRKEGKVTF
                     YQARKS"
     gene            complement(503..586)
                     /locus_tag="P5219_05605"
     tRNA            complement(503..586)
                     /locus_tag="P5219_05605"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(551..553),aa:Leu,seq:tag)
     gene            630..2306
                     /gene="cls"
                     /locus_tag="P5219_05610"
     CDS             630..2306
                     /gene="cls"
                     /locus_tag="P5219_05610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HRX26265.1"
                     /translation="MGRRFFSVRERERTCVFSRSLRPKEGCAALKRSKGTGFLLLFLF
                     GFATALFLFSRWEELAAAGRVLFGDGGSFSGAFRHFLGSLPALIREYSSAFLWAYGLF
                     IAGVIFLEEKNPDRTIAWLVVLILLPVVGFVLYLFFGPDIRGRGYFRRLKRKKRRIRQ
                     EGTPQHGGDRKEIMSGSPEQKTALMLSKSARALLTTGNSVKMLLNGEETFSAITGALS
                     RAEKYIHMEYYSIANDTIGNTIKDILIRKAGEGVRVNVIFDSVGSWDLGTRYVVALKN
                     AGVRVHSFLPVSFPRLRRELNFRNHRKIAVIDGTIGFMGGLNIGDMYITGEPSIGFWR
                     DTHIELTGPAVSCLDDIFRADWTFCDKKPLEEHAPVPAPPAGKVPVQIAASGPDNNWK
                     SILQGYFSLITNARKSVWLCTPYFVPGEPLMTALAVAAMSGVDVRVMIPHKTDHAIVY
                     WASLSNVEELLKAGVKVYRYEKGFIHSKILIADGSSVSVGTANLDARSLEINFEVQAF
                     IYDASVARDFIRAYENDMEESSQFRLHEWLRRPFRQKVLESAGRLWSSQL"
BASE COUNT          557 a          698 c          652 g          525 t
ORIGIN      
        1 agaccttgat ctcatcctgg aggcagccat ctgggctccc agcggtcaca acgcccagcc
       61 ctggcatttc ctcgtgattc aggacaaggg aaaaatcgac gaaatgagca caaagaccat
      121 cagcataatg gccgactccc ccatcgaatg ggtcaggaag ctggcgggaa gagaaggata
      181 ccatcttttc cacagggctc ccacggtgat agtcgtctcg gggaaaagac cgggcagcga
      241 tcttctgttc ccccacgccg actgttccgc ggccatacaa aacatgcttc ttgcagccga
      301 atccctgaat atcgggagct gctgggtcgg cctcaccggg tttctcttcg gaatcccgga
      361 agaagtggaa gctctcggga ttcctgaaga ctgccagcct ctgtactcag tctgtttcgg
      421 ctacaaggac gattccttcg ttccgccggt tcctcccagg aaagaaggga aggttacctt
      481 ctaccaggcc cggaaatcat aatggtgcga gaggggggac tcgaaccccc acgccctgag
      541 gcatcagatc ctaagtctga cgtgtctgcc gttccaccac tctcgctccc gcgttccatg
      601 tcgtggcatg ataacattat tgacggtttt tgggaaggcg ttttttctcc gtcagggaaa
      661 gggaaaggac ctgtgttttt tcccggagcc tccgaccgaa agaagggtgc gccgctctga
      721 aaagatcaaa aggaacagga tttcttcttc tctttctctt cggcttcgcc accgcgcttt
      781 tcctcttttc caggtgggag gagctggctg cggcagggag ggtcctcttc ggcgacggag
      841 gatctttcag cggagccttt cggcattttt taggcagtct tcccgccctc atccgtgaat
      901 attcttccgc tttcctgtgg gcttacggcc tctttatcgc cggagtgatc ttcctcgagg
      961 agaaaaaccc ggacaggacc atagcctggc ttgtagtcct cattcttctt cctgtggtcg
     1021 gtttcgttct ctatcttttt ttcggacccg acatccgggg acggggttac tttcggaggc
     1081 tgaaaaggaa gaagcgtcgt atccgccagg aagggacacc ccagcacgga ggcgacagga
     1141 aagagatcat gtcgggatct ccggaacaaa aaacggccct catgctttcg aaaagcgcca
     1201 gggccctcct caccacgggg aattcggtca aaatgctcct caacggggag gaaaccttct
     1261 ccgccataac gggtgccctc tcgcgggctg aaaaatacat ccacatggag tattattcca
     1321 tagccaacga caccatcgga aacaccataa aggacatcct cataagaaaa gccggagaag
     1381 gggtacgggt caacgtcatc ttcgacagcg tgggaagctg ggatctcggg acacggtacg
     1441 tcgtcgccct gaaaaatgcg ggcgtcaggg tccactcctt tcttcccgta tctttcccga
     1501 ggctccgcag agagctcaac ttcaggaacc acaggaaaat cgcagtcatc gacggaacaa
     1561 taggtttcat gggaggattg aacatcggcg acatgtacat cactggagag ccttccattg
     1621 gattctggag agacacccac atcgaactga ccgggcctgc cgtgtcatgt cttgacgaca
     1681 ttttcagggc cgactggacc ttctgcgaca aaaaacccct tgaagaacac gctccagtcc
     1741 ccgctcctcc cgcagggaaa gtcccggtgc aaattgcggc aagcggtcct gacaataact
     1801 ggaaatccat cctccagggc tacttctcgc tcatcaccaa cgccaggaaa tccgtctggc
     1861 tgtgcacccc ctacttcgtc ccgggtgaac ctctgatgac tgccctcgcc gttgctgcga
     1921 tgagcggagt tgacgttcgg gtcatgatac cccacaaaac ggaccacgcc atagtctact
     1981 gggcctccct ctcaaacgtg gaggagctcc tcaaagcggg agtcaaggtc taccgctacg
     2041 aaaaaggatt catccactcg aaaatcctca tcgcggacgg ctcctccgtg tcggtgggaa
     2101 cggcaaacct ggacgccagg agtcttgaga tcaatttcga ggtacaggcg ttcatatatg
     2161 acgcctccgt ggcgcgggat ttcatccggg cgtatgaaaa cgacatggag gaaagctccc
     2221 agttccggct gcacgaatgg ctccggcgcc ccttcaggca gaaggtgctg gaatccgccg
     2281 gacgtctctg gtcatcccag ctctgagaag aaagagcgag aagggcccgg attggattcc
     2341 gggcccttct ctgtatgaaa acatgtcgat aattttaaat ggtggactgt gagggactcg
     2401 aacccacgac ccgctgatta agagtcagct gc
//
LOCUS       DAOTHD010000277         2431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_51469_length_2431_cov_3.146886, whole genome shotgun sequence.
ACCESSION   DAOTHD010000277 DAOTHD010000000
VERSION     DAOTHD010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51469_length_2431_cov_3.146886"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="P5219_05615"
     CDS             <1..329
                     /locus_tag="P5219_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRX26266.1"
                     /translation="SATGTSPEIVLVHGVSPVSAEEAPAMLNAAEEALEEIRDEIASW
                     GLEVSTSLLAGDPATDLPGRIEELAPTALAIGLPAAGELGRLVPGSVAEGLIESTRCP
                     LVVFPL"
     gene            complement(326..1426)
                     /gene="sfsA"
                     /locus_tag="P5219_05620"
     CDS             complement(326..1426)
                     /gene="sfsA"
                     /locus_tag="P5219_05620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HRX26267.1"
                     /translation="MKKIEKNVAVFRAADPEMLRRARFIDRPNRFLVRCELDGHPVRA
                     FLPNPGRLWEILLPGTELLLAEDGGSETRKTAFTAVAALRGESAVLLHTHTANDAAGW
                     LIRNDRIPGLEGWTVKKREASFGHSRFDFLLEKEGRLLLLEVKSCTLFGHELAMFPDA
                     PSDRGRKHVEELWKLAGEGYGAAVLFLVQSSRPNFFLPDFHTDPKFAQSLFRAKERLQ
                     VFPIAVEWNNALELTGEPKLLPVPWEVYERHRGDEGDCLVLADAGENGFWAAGFRTEK
                     LSSFAGRALRRRGKDFPFPPGEDGGVRVISIRSSCSAFQDLQNDISSFAAGEIIKDGT
                     RYYAFKEPPMKNAGFVEMLLRRKTDLLLERLP"
     gene            complement(1525..>2431)
                     /locus_tag="P5219_05625"
     CDS             complement(1525..>2431)
                     /locus_tag="P5219_05625"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRX26268.1"
                     /translation="YDPEEIICTVGVSEGVYVSLSSFLDPGDEVLVPDPSWVSYFHVP
                     TMNGAVPKGYPLREENAFQIDVDDLEKLVSPRTKMMVVLDPSNPVGAVQEKETLARIA
                     EFAVKHNLLVISDEIYEKIIYDGKKHYSIAAFPGMRERTIVLNGFAKAYAMTGWRIGY
                     IAAPAELISVMNRMHMYIVTHTSAQAQWGALAALEGPQEPVAGMVEEFRKRRDYVCRR
                     IGGMKKVSCRVPGGAFYVFVNIKETGMGAEEFTKYLIREAHVAVVPGTAFGSHGEGYV
                     RLSYAVSMENLEKSMDRIEKAVASL"
BASE COUNT          518 a          707 c          664 g          542 t
ORIGIN      
        1 tgtcagccac gggaacatcc cctgagatcg tcctcgtgca cggggtatcc cccgtgtcgg
       61 ctgaagaagc tcccgcgatg ctgaatgcgg cggaagaggc cctggaggaa atccgggatg
      121 aaattgcctc atggggactt gaagtttcaa cgagccttct tgcgggagat cccgccactg
      181 atcttccggg ccggatagag gagcttgccc ctacggcgct tgccatcggg cttccagcag
      241 cgggagagct cgggcggctc gtccccggat cggttgcgga aggactgatc gagagcaccc
      301 ggtgtcccct ggtggtcttt cctctctagg gaagccgctc cagcaggaga tcagttttgc
      361 ggcggaggag catttccacg aatccggcgt tcttcatggg aggctcctta aaagcataat
      421 accgggtccc atcctttatg atttctcccg ctgcaaagga ggagatatcg ttttgcagat
      481 cctgaaaagc tgaacaggat gaccggatgg agataacccg cactccccca tcttcccccg
      541 gagggaaggg aaaatctttg cctctcctgc gcagggctct ccctgcaaaa gacgagagct
      601 tttccgtacg gaagcctgcc gcccagaatc cattttctcc ggcgtctgca aggacgagac
      661 agtccccctc gtcgccgcgg tgccgttcat agacctccca tggcacaggc aggagcttcg
      721 gctctcccgt aagttcaaga gcattgttcc attcaacggc aatggggaat acctgaagcc
      781 tctccttcgc cctgaacagg ctttgggcaa acttggggtc agtgtgaaaa tccgggagga
      841 aaaaattggg gcgggaggat tggaccagga aaagaaccgc cgctccgtac ccctctcccg
      901 ccagtttcca gagttcttcc acgtgcttcc gcccgcggtc cgacggcgcg tcgggaaaca
      961 tagccagctc atggccgaaa agagtacagg atttcacctc cagaaggagg agacgtcctt
     1021 ctttttcaag gagaaagtcg aaccgggaat gaccgaatga tgcctccctt ttttttaccg
     1081 tccacccctc cagcccgggt atcctgtcgt tcctgatgag ccacccggct gcgtcattgg
     1141 cagtgtgggt atggagaagg acggctgatt ctcctctcaa ggcggcaaca gccgtgaaag
     1201 cggtttttct ggtctcgctc ccgccgtctt cggcaaggag aagttccgtc cccgggagca
     1261 gtatttccca tagcctcccg ggattgggaa gaaatgccct gaccggatgg ccgtcaagtt
     1321 cacagcggac cagaaagcgg tttggcctgt cgatgaatcg ggcgcgccgg agcatttccg
     1381 gatccgcagc acgaaacacg gcaacattct tttcgatttt cttcatctcc tttttcccgg
     1441 tcaactgtac caaaaaaaag atccgcggga aagaagactc tccttcccgc ggacgttctc
     1501 tgacactcct gcttgcagaa tcctttacag ggaagcgaca gctttttcga ttctgtccat
     1561 gcttttttcc aggttctcca tggaaacagc gtaggaaagt ctcacatacc cctcgccgtg
     1621 ggagccgaag gccgttccgg gaactacagc cacatgagcc tcccggataa ggtatttcgt
     1681 gaattcctcc gcacccatgc ccgtttcctt tatattcacg aaaacataaa aagcaccgcc
     1741 gggaacacga caggagactt ttttcatgcc cccgattctg cggcagacat agtccctgcg
     1801 ctttctgaat tcctcaacca tgccggctac cggttcctga ggcccctcga gggccgctaa
     1861 tgctccccac tgggcctggg cggaggtatg ggtgacgatg tacatatgca ttctgttcat
     1921 cacggagatc agctcggctg gagcggcgat gtagcctatc cgccagccgg tcatggcata
     1981 ggccttggcg aagccgttga gcacgatggt ccgctccctc attcccggga aggcggcgat
     2041 gctgtaatgc ttctttccgt catagattat tttttcataa atctcgtcgg agatcacgag
     2101 aaggttgtgc tttacggcaa attcagcgat cctggcgaga gtttcctttt cctgaaccgc
     2161 tcccacaggg ttggacggat cgaggaccac catcatcttc gtcctgggtg aaaccagttt
     2221 ttccaggtca tccacgtcga tctggaaggc attctcctcc ctgagcgggt agcccttcgg
     2281 aacggcgccg ttcatggtgg gaacatggaa gtagctcacc caggaagggt caggaacaag
     2341 cacttcgtca ccgggatcaa ggaaactgct cagggagacg tacactcctt cggaaacacc
     2401 aacggtgcag atgatttcct cggggtcata t
//
LOCUS       DAOTHD010000278         2431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_51476_length_2431_cov_2.590488, whole genome shotgun sequence.
ACCESSION   DAOTHD010000278 DAOTHD010000000
VERSION     DAOTHD010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51476_length_2431_cov_2.590488"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="P5219_05630"
     CDS             complement(<1..720)
                     /locus_tag="P5219_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRX26269.1"
                     /translation="MNRRVLFLFYLLFLVLPRGQWASGAEPLSLEKTLSLAAENNPIL
                     AAGRQRIVQARERIHQATAASLPQLGVSLLYQTADKDPFNPVFIEGQQVGYAQAGFRT
                     TWKAALTLSQLIYSGGAVRYSVDSRRLALEGVEAAEKRTAQGVEWAATSAWYDLRRAA
                     GRLSVAEDTLALTKEHLRHVQALFRNGVVAKNDVLRVEVSVSEAELGRIRAMNAVDVA
                     WHGLSRAVGTSLRPAFTLPAED"
     gene            complement(710..1318)
                     /locus_tag="P5219_05635"
     CDS             complement(710..1318)
                     /locus_tag="P5219_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRX26270.1"
                     /translation="MAEETRESRKAILRAAREGFAARGFYGTSMEYITRNAGVSKGAV
                     YWYFPGKWEVYKAVVAEEAEKLKEIVLPPGLEFPTGEAMDFFLTRGERLIDLFAEDPV
                     CRLLFIHLQLEAMRGREKMIELVTALRASITDDVISVIEAVFPGDSLSRLGISHRDLV
                     NMFVGILNGIILNIELTITREEAGRNWRFLIQSVLGGISNEP"
     gene            complement(1441..1836)
                     /gene="rpsI"
                     /locus_tag="P5219_05640"
     CDS             complement(1441..1836)
                     /gene="rpsI"
                     /locus_tag="P5219_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRX26271.1"
                     /translation="MQNTAYCWGTGRRKCALARVRVRPGDGTIKVNERTVEDYFPRVA
                     WQLSALQPLKSAGLEGKVDVFVRATGGGLTGQAGAVRLGIARALLKLNPELRPVLKKN
                     GFLTRDSRMVERKKFGQKGARGLRQFSKR"
     gene            complement(1861..2292)
                     /gene="rplM"
                     /locus_tag="P5219_05645"
     CDS             complement(1861..2292)
                     /gene="rplM"
                     /locus_tag="P5219_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRX26272.1"
                     /translation="MSDSRSFMAVKEKVEHQWYIVDATDKPLGRIASQVAKILMGKNK
                     PTYTPHVDCGDFVIIINAEKAKLTGNKRLKSTVHYYTGYLGGFRSATYGEMMDKKPVQ
                     LMERVVKGMLPRTRLKLHRKLKVYAGPEHPHAAQRPVAIEV"
BASE COUNT          483 a          726 c          679 g          543 t
ORIGIN      
        1 gtcctccgcc ggaagggtga atgcggggcg gagagatgtt ccgactgccc tggaaaggcc
       61 gtgccatgcc acgtccacgg cgttcatcgc ccggatgcgt cccagctccg cctctgagac
      121 ggaaacttcc acccggagga cgtcgttttt cgccaccacg ccgttccgga agagagcctg
      181 cacgtgccgg aggtgctctt ttgtcagagc gagggtatct tccgccacag aaagccttcc
      241 cgccgcccgc cgcaggtcgt accatgctga cgtcgccgcc cattccactc cctgggcggt
      301 gcgcttctct gccgcctcga cgccttcaag ggcaagccgg cgggaatcca cggaataccg
      361 gacggcaccg ccgctgtata tgagctggct cagcgtcagg gcagccttcc aggtggtccg
      421 gaagccggcc tgggcgtacc cgacctgttg tccttcaata aagacaggat taaacggatc
      481 cttgtcggca gtctgataga gaagggagac accaagctgg ggcagcgaag cggcggtcgc
      541 ctggtgtatg cgttcccgtg cctgtacgat cctctggcgg cctgctgcca gaatcgggtt
      601 gttttcagcg gccagggaaa gggtcttttc cagggagaga ggctccgcac cgctggccca
      661 ttgcccccgg gggagaacca ggaagagaag gtagaacagg aagaggactc tacggttcat
      721 tgcttatccc tcccagaacc gactggataa ggaaccgcca gttccgcccg gcttcttccc
      781 ttgtaatcgt aagctcgatg ttgagaatga tgccgttcag gatgccgacg aacatattga
      841 ccaggtcgcg gtggctgatt ccgaggcgcg agagagaatc accgggaaaa acggcttcga
      901 tgaccgagat cacgtcatcg gtaatggagg cccgaagagc ggtgacaagc tctatcattt
      961 tctcccggcc ccgcatcgct tcaagctgca ggtggataaa gagcagcctg catacagggt
     1021 cttccgcgaa gagatcaata aggcgctcgc cgcgggtgag aaaaaaatcc atagcttccc
     1081 ctgtcggaaa ttcgagtccg ggaggaagaa caatttcctt gagtttctcc gcctcttcag
     1141 ccaccacggc cttgtacact tcccacttgc cggggaaata ccagtaaacc gctcccttgc
     1201 tgactccggc gttcctggtg atgtactcca tgctggtacc gtaaaatccc ctggcggcaa
     1261 agccctcccg ggccgccctg aggatggctt ttctcgattc tcttgtttct tcagccacta
     1321 ttatccctcc cgactattca ttcgagatca ctctactcca taccgaatgg tcggtcaaga
     1381 aaggagataa aaaaggacag gccgaggcct gtccgaaaaa acactgcaat gattgtccga
     1441 ttagcgtttg gagaactgcc gaagtccccg ggcacccttc tggccgaact ttttccgctc
     1501 caccatgcgt gagtccctgg taaggaatcc gttcttcttc agaacaggac gtagttctgg
     1561 attgagcttc aaaagagccc tggcgattcc gagcctgact gctccggcct ggccggtaag
     1621 gccgccgccg gtggcgcgga cgaaaacatc gacctttccc tcgagtccgg cggacttcag
     1681 cggctgcaga gcgctgagct gccatgcaac ccgcgggaag taatcctcca ctgttctttc
     1741 gttgacttta attgtcccgt cgccggggcg aacccgcacg cgagccaggg cgcattttct
     1801 tcttccggtt ccccagcagt atgctgtatt ctgcatcgag tcttttcccc ctcctcaaaa
     1861 ttacacttct atggcaacgg gccgctgcgc cgcgtgagga tgctccggtc ccgcatacac
     1921 tttgagcttc cggtggagct tgagccttgt cctgggaagc attcccttga cgacccgctc
     1981 catgagctgc acaggtttct tgtccatcat ctctccgtag gttgcggaac ggaatcctcc
     2041 aagatacccg gtgtagtaat ggacggtgct cttgagcctt ttgttgcccg tgagcttcgc
     2101 tttctcggcg ttgatgatta tgacgaaatc gccgcagtca acgtgggggg tgtaggtagg
     2161 cttgttcttc cccatgagaa tcttggcgac ctgggaagca atacggccaa ggggcttgtc
     2221 cgtggcgtcg actatgtacc actggtgctc gaccttttct tttacggcca taaaggaacg
     2281 actgtccgac atggaatttc ctccttattc tctttcctgt ttcatttttc tatagtcaaa
     2341 gatcacacga acgccgatgg tggaagcgcc gccccaagca tctcgggaac gtggcagggg
     2401 tccccggggc ttgctttctc cgccgacgcg g
//
LOCUS       DAOTHD010000279         2420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_51785_length_2420_cov_3.107822, whole genome shotgun sequence.
ACCESSION   DAOTHD010000279 DAOTHD010000000
VERSION     DAOTHD010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51785_length_2420_cov_3.107822"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>567
                     /locus_tag="P5219_05650"
     CDS             <1..>567
                     /locus_tag="P5219_05650"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRX26273.1"
                     /translation="EWAPPAAETVDGRGKAALLPGFVNAHTHAAMSLLRGIGEEAPLM
                     EWLKEKIWPAEAKLKGEHVSWGTKLALLEMAANGITAFGDMYFFMDSVVEASLEMGMK
                     CAVSRGIVGPEQVKLDEGLQLAETWKGREEFVTVQLAPHAPYTVPLSFLKEIVAAAKD
                     RNLGIHFHFLEAEWETGYLRDEMKMTPA"
     assembly_gap    568..577
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <578..1191
                     /locus_tag="P5219_05655"
     CDS             <578..1191
                     /locus_tag="P5219_05655"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRX26274.1"
                     /translation="LGLKPILYGDQVRQAVLAHCVWLDPAVLDEVDFSRMTIAHNPKS
                     NQKLGSGMMDLPGMMEKTRKIALGTDGAASNNRLDVWGEMRSAALVSKGISKDPTAVP
                     ARDVLRMATFEGAEALGFEKKGMIREGWAADFVMVDLDRPEYVGIDGENAAHFFVYAG
                     SSADVTGTMVAGKWLYRDGNFPGADAEKILAKAREMRDNLLKG"
     gene            1678..>2420
                     /locus_tag="P5219_05660"
     CDS             1678..>2420
                     /locus_tag="P5219_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HRX26275.1"
                     /translation="MQWRSGKEVRSLFIDFWVSKGSRHFPSFSLIPDDPSLLFTIAGM
                     VPFKPYYLGIQKPEAPRAVTSQKCVRTNDIDNVGRTARHHTFFEMLGNFAWGDYFKKE
                     SITWGWEFLTEVIGLEPDRMSVTIYEDDDEAYDAWHKLVGIPDQRIFRFGKDDNFWFM
                     GNQGPCGPCSEIMYDQGPEFSCGRPECAVGCDCDRYLEIWNHVFTQFDLQKDGTLVPL
                     PKKNIDTGMGLERLTSIVQRVRTDFETDL"
BASE COUNT          513 a          675 c          788 g          434 t
ORIGIN      
        1 cggaatgggc tccgcctgcc gccgaaaccg tggacggcag agggaaggcc gcccttcttc
       61 cgggctttgt aaacgcccac acccatgcgg ccatgagcct gctgcgcggg ataggggaag
      121 aggcccccct catggagtgg ctgaaggaga agatctggcc cgccgaggcg aagctgaagg
      181 gcgagcatgt ctcctggggc acaaagcttg ctctgctcga gatggcggcg aacggcataa
      241 cggccttcgg tgatatgtat ttcttcatgg attccgtagt ggaggcatcg ctggagatgg
      301 gcatgaagtg cgccgtcagc cggggtatcg tcggaccgga gcaggtgaag ctggacgagg
      361 gcctgcagct tgccgaaacc tggaagggcc gggaggagtt cgtcacggtg cagctcgccc
      421 cccacgcacc ctatacggtg cctctgtctt ttctgaagga gatcgtggcg gcggcgaagg
      481 accggaacct ggggattcac ttccatttcc tggaggcgga gtgggaaacg ggatatctga
      541 gagacgagat gaaaatgacc ccggcggnnn nnnnnnnggc tcggcctgaa gccgatcctg
      601 tacggagatc aggtccggca ggcggtgctg gctcactgcg tgtggctcga cccggccgtt
      661 ctggacgagg tggatttctc ccggatgacc atcgcccata acccgaagag caaccagaag
      721 ctgggcagcg gtatgatgga cctgcccggc atgatggaaa agacacggaa aatcgccctg
      781 ggaacagacg gtgcggccag caacaaccgt ctcgacgtgt ggggggagat gcggtccgcc
      841 gcccttgtct ccaaggggat ttcaaaggac cccaccgccg ttcccgccag ggatgttctg
      901 cggatggcca ccttcgaggg ggcggaagcc ctcgggttcg agaagaaggg gatgatccgg
      961 gagggctggg ccgccgactt cgtgatggtg gacctggaca ggccggagta cgtggggatc
     1021 gacggggaaa acgcggctca tttcttcgtg tacgccggaa gctccgccga tgtgacggga
     1081 accatggtcg ccgggaaatg gctgtaccgg gacgggaact ttcccggtgc ggacgctgaa
     1141 aaaatcctcg ccaaggcgcg ggaaatgcgg gataatctgc tgaaggggta gggattttcg
     1201 ggggacgaaa cggtctgtcg gctcgaaaga accgcttttg tctgtcatcc tgagcggagc
     1261 gatcgcgtcc ccggagggga aggatctgct cttgttgctg aaggacccaa ccccgagatc
     1321 cttcggccaa ggaaccggcc tcaggatgac aaaaaggcga gatccttcgg ccaaggaacc
     1381 ggcctcagta tgacaaaaag gtgagatcct tcgcccaata gagccggcct caggatgaca
     1441 aagaaccggg atccctcccc ttcggggatg ctccgcgatc gcatccgctc gggatgacaa
     1501 acaatagacc ggcctcatgg tttgtcattc cgaagggcga agcccgagga atctgctttt
     1561 gtcctggaag agtccaaccc cgagatcctt cgggcaaaaa cgccctcagg atgacagcat
     1621 gagcagaaca gcatcagttt ttcagccata gacagcattc agggaggaag aatattcatg
     1681 cagtggagat caggcaagga agtacgttcg ctattcattg atttctgggt gtccaagggg
     1741 agccggcatt tcccgagctt ttcgctcatt cccgacgatc cttccctgct gttcaccatt
     1801 gcgggaatgg tgcccttcaa accctactac ctcggcatcc agaaacccga ggcgccccgg
     1861 gcggtgactt cccagaagtg cgtccgaacc aacgacatcg acaacgtggg ccgcacggcc
     1921 cggcaccata ccttcttcga gatgctgggg aacttcgcct ggggggacta tttcaagaaa
     1981 gagtccatca cctggggatg ggaattcctg acggaagtga tcggtctcga gccggaccgc
     2041 atgtcggtga ccatctacga ggacgacgat gaggcctacg acgcgtggca caagctcgtg
     2101 ggcattcccg accagcgcat cttccgcttc gggaaggacg acaatttctg gttcatgggg
     2161 aaccagggcc cctgcggtcc ctgttcggag atcatgtacg accaggggcc ggagttttca
     2221 tgcggcaggc cggagtgcgc cgtggggtgc gactgcgacc ggtacctgga gatctggaac
     2281 cacgtgttca cccagttcga cctccagaag gacggaaccc tggtgcccct gccgaagaag
     2341 aacatcgaca ccggcatggg gctcgagcgg ctgacgtcca tcgtccagcg ggtgcggacc
     2401 gacttcgaga cggacctctt
//
LOCUS       DAOTHD010000280         2420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_51789_length_2420_cov_2.688795, whole genome shotgun sequence.
ACCESSION   DAOTHD010000280 DAOTHD010000000
VERSION     DAOTHD010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51789_length_2420_cov_2.688795"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..1280)
                     /locus_tag="P5219_05665"
     CDS             complement(54..1280)
                     /locus_tag="P5219_05665"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRX26276.1"
                     /translation="MSKKGKVVLAYSGGLDTSVAVMWLTEQGYDVVTMTADVGQQAVD
                     LEAAKNKALRSGAVKAYVLDLKKVFVDKFVWPSLKANGMYQSTYPLNSALSRPLIAEV
                     MAEIAKKEGAVAVAHGCTGKGQDQVRIEVCTNALNPALQVLAPVRDWHFTREAEMEYA
                     QAHGIPVLATAASPYSIDDNIWGRSIECGILEDPWNEPPSDAYTLTVDPWDAPDEPEY
                     VEIAFNKGVPAALDGVKMDGPTLIETLNARAGRHGVGRIDMVEDRLVGFKSREVYECP
                     AAITLINAHRALETLTLDKKVIAAKKELEVKFSELTYEGYWFSPLKDAINSFIETTQE
                     YVSGLVKVRLYKGEAVVRGMKSLDSIYSHTLATYSEGDAFDHSAAVGFIKIWGLPIKT
                     WRQVHESKEAIEISAQ"
     gene            complement(1582..2367)
                     /gene="surE"
                     /locus_tag="P5219_05670"
     CDS             complement(1582..2367)
                     /gene="surE"
                     /locus_tag="P5219_05670"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRX26277.1"
                     /translation="MKLLITNDDGVFAPGIVELASALCRLGHECAVTAPDRERSSVGH
                     SITLTRPLRLWKAAPGPYPPECPVFACDGTPSDCVVLGIEEVMAEPDLVVSGINRGPN
                     LGDDLTYSGTVSAAMEGVILGLGAIAFSLDCHESDQDQHYETASSVAGALIGFISSNP
                     LPRGVLLNVNVPNVPLSLLRGVRVTRKGVRLYEGKVTRFTDPGGRTYYWVAGRPEDQL
                     EEGSDVWAAANGYVSVTPVHMDMTHFPSIETLTADGAEKINFK"
BASE COUNT          483 a          720 c          690 g          527 t
ORIGIN      
        1 aaagaccggg gtctccctag gaggccccgg tcttcatgac tatttttaaa ccgttactgg
       61 gcggaaatct cgatggcttc cttggactcg tgcacctgcc tccatgtctt gatgggaagt
      121 ccccatatct tgatgaagcc cacggcggcg ctgtggtcga aagcgtctcc ctccgagtag
      181 gtggcaaggg tgtggctgta gatggaatcc agagacttca tgcccctcac taccgcttct
      241 cccttgtaga gcctgacttt cacgaggccg gacacatact cctgggtcgt ttcgataaag
      301 gagttgatgg cgtccttcag gggtgagaac cagtaccctt cataggtcag ttcgctgaac
      361 tttacctcaa gctctttttt ggccgcaatg acttttttgt caagagtcag ggtctcaaga
      421 gcccggtgtg cgttgatcag ggtaatggcc gccggacatt cgtagacttc cctgctcttg
      481 aatccgacaa gccggtcttc aaccatatcg atccttccga ctccgtgccg tcctgccctg
      541 gcgttcagcg tttcgatgag ggtcggaccg tccatcttca cgccgtcaag ggctgcgggt
      601 acccccttgt tgaaggctat ctcaacgtat tccggttcat cgggggcatc ccacggatcg
      661 acggtcaggg tgtaagcgtc cgaaggaggc tcattccagg gatcctcgag aatgccgcat
      721 tcgatggacc tgccccagat attgtcgtca atgctgtagg gagaggcagc cgttgccagc
      781 acgggaatgc cgtgggcctg ggcgtactcc atctccgcct cgcgggtgaa gtgccagtcc
      841 cggacggggg caagcacctg cagcgcggga ttgagcgcat tggtgcagac ctcgatacgg
      901 acctggtcct ggccttttcc ggtgcagccg tgagcaacgg caactgctcc ttccttcttc
      961 gcgatctccg ccatgacctc agcgatgaga ggccgcgaaa gggccgagtt cagggggtag
     1021 gtgctctggt acatgccgtt tgccttcagg gagggccata cgaatttgtc cacgaagacc
     1081 ttcttgaggt cgagaacata ggctttcacc gctccgcttc gcagcgcctt gtttttcgcc
     1141 gcctcgaggt caaccgcctg ctggcccacg tcggcggtca tggtgaccac gtcgtacccc
     1201 tgttccgtga gccacatgac ggccacggac gtatcaagac caccgctgta cgccagaacc
     1261 actttgccct ttttcgacat gaaaatccct ccaggatact ctaaaaataa aaacccgccc
     1321 cggtaagggc gggcgtttgc ccgactgcca ccctgccttg cgcgcaatgc gcccggtgtc
     1381 tgtttctact tggccggaag cctttcttcg aaacaggctc agggaccctc ttcctccctg
     1441 ccggagccgg aaaagctttc agccgcggct cttcctctct gttgcccgca aaggagtact
     1501 cttccccgtc atcgcctttc aaaacgccga cgaagaagat tatacagagg aataaacaaa
     1561 tgtcaagtaa aatgaattca gttacttgaa gtttattttt tcagcaccgt cggcggtgag
     1621 cgtttcgatg gaagggaaat gggtcatgtc catatgcacg ggagtcaccg aaacgtatcc
     1681 gtttgcggcc gcccagacgt cgctcccctc ttcgagctgg tcctcggggc gacccgccac
     1741 ccagtagtag gttcttcccc ctggatcggt aaagcgggtt acttttccct cgtacagccg
     1801 gacgcctttc cgggtgaccc tgactcccct gagcagggac aggggaacat tgggaacatt
     1861 cacgttcaga aggactcccc ggggaagagg gttggagctg atgaatccga tcagggcccc
     1921 cgcaaccgaa gaggcggttt cataatgctg atcctgatcg ctttcatgac agtccaggga
     1981 gaaggcgatg gcccccagtc cgagaatgac cccttccatg gctgcggata ccgttcccga
     2041 ataggtcagg tcatcgccca ggttcggtcc cctgttgatg ccggagacca ccaggtcggg
     2101 ttccgccatg acctcctcta tcccaagaac gacacagtcg gacggtgtgc cgtcgcaggc
     2161 gaaaacgggg cattccggag gatagggtcc cggggcggct ttccacagcc ggagaggccg
     2221 ggtcagcgtg atggagtgac cgacgctgct ccgttcccgg tcgggagcgg tgacggcaca
     2281 ttcgtgcccc agccggcaga gggccgaggc cagttccacg atgcccgggg caaaaacgcc
     2341 gtcgtcattg gtgataagaa gtttcatgac ggaactccct acagaaggat gagcctgaga
     2401 aaaaacatca ccacgggccc
//
LOCUS       DAOTHD010000281         2418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_51836_length_2418_cov_3.323318, whole genome shotgun sequence.
ACCESSION   DAOTHD010000281 DAOTHD010000000
VERSION     DAOTHD010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51836_length_2418_cov_3.323318"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="P5219_05675"
     CDS             complement(<1..511)
                     /locus_tag="P5219_05675"
                     /inference="COORDINATES: protein motif:HMM:NF021210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2400 family protein"
                     /protein_id="HRX26278.1"
                     /translation="MRKVRPPKKDTLSEEARNLAGFLPRFEEAYRRLNRREFISPDPL
                     EKLYLFETVEEREIVGLAISSIAYGRVAQILRNADRLLSVMGPSPRTFLMKTSAGDLS
                     RLLGGFRHRFTSGDEMASFLAGIGKVLRKYGRLDAFFEDCIRRSGDLMGGAALFSGEI
                     LAGGGLGKSF"
     gene            complement(531..1058)
                     /locus_tag="P5219_05680"
     CDS             complement(531..1058)
                     /locus_tag="P5219_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRX26279.1"
                     /translation="MDAHIDPRALFTLNYGVYILSTSYEGKKNGQIINALMQLTADPI
                     CMAACLHRDNYTTELVEKSGRFSISVLEDAVPLKFIGVFGFRCGREFDKFGECTYEIG
                     ESGLPLVTEYSLAGIELKVLSVQDVFTHKLIVGQVEKAQLLKEGTPLTYANYHRVKKG
                     KSPANAPSAVFNQVK"
     gene            complement(1154..1648)
                     /locus_tag="P5219_05685"
     CDS             complement(1154..1648)
                     /locus_tag="P5219_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HRX26280.1"
                     /translation="MISKKIEDAFNDQINAELYSAYIYLSMSAYLNSADLNGMAHWMK
                     VQAEEEVEHAMKFASYIVERGGRVKYKAIECPQEEWASALEVFKGAYEHERYVTRRIN
                     DLMDLSVAEKDYASQVFLQWFVSEQVEEEANADEIVKKLEMIGEGRHGIYMLDKELGG
                     RKAD"
     gene            complement(1651..2013)
                     /locus_tag="P5219_05690"
     CDS             complement(1651..2013)
                     /locus_tag="P5219_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HRX26281.1"
                     /translation="MKKLDVFKCDLCGNVVELLHVGGGDLVCCGQPMKLLEEKSADSA
                     TEKHVPVVEGNKVKVGSVPHPMTNEHYIEFIEIMDGERICRKFLNPGEAAEAVFDSFT
                     PTKSREYCNIHGLWKVDL"
     gene            2208..>2418
                     /locus_tag="P5219_05695"
     CDS             2208..>2418
                     /locus_tag="P5219_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26282.1"
                     /translation="MKPTMKTNAVALVFSLCFLFSAEAGVSGRKTVLLLPTLNFTEYE
                     IWESKFYPVNVLERKMNEHLASLLRR"
BASE COUNT          543 a          673 c          587 g          615 t
ORIGIN      
        1 gaaaactctt cccgagtccg cccccggcaa gaatctcccc ggagaacagc gcggccccgc
       61 ccataaggtc cccgctcctg cggatgcagt cttcaaaaaa agcgtcgaga cggccgtact
      121 tcctgagtac tttcccaatt cccgcaagaa aagaggccat ttcatccccg gatgtgaacc
      181 tgtgacggaa ccctccaagg agacgggaaa gatcccctgc ggatgttttc ataagaaatg
      241 tcctggggga tggccccatg accgaaagca gcctgtccgc attcctgaga atctgggcaa
      301 cccttccgta ggcaatggac gatatggcaa gacccacgat ctccctttcc tccactgttt
      361 caaacaggta gagtttttcc aggggatccg gagaaatgaa ttcccttcgg ttcaatctgc
      421 ggtatgcttc ctcgaagcgg ggcagaaacc cggccagatt tcgtgcctct tcggacaggg
      481 tatccttttt cggaggcctc accttcctca ttctcctgaa aaagcggact tcacttcacc
      541 tggttgaaga ccgcggaggg ggcattcgca ggggattttc cttttttcac cctgtggtag
      601 ttggcatagg tgagaggagt cccttctttc agcagctggg ctttctccac ctggcccacg
      661 atcagtttgt gggtgaaaac gtcctgcacg gaaagcacct tgagttcaat gccggcaagg
      721 ctgtattcgg ttacgagggg aagaccgctt tccccgattt cataggtgca ttcgccgaat
      781 ttgtcgaact cacggccgca gcggaagccg aaaactccaa tgaatttgag cgggacggcg
      841 tcctcgagga cggaaatgga aaacctgccg cttttttcca cgagctccgt ggtgtaattg
      901 tccctgtgaa gacaggccgc catgcagatg gggtccgcgg tcagctgcat gagggcgttg
      961 atgatctggc cgttcttttt cccttcgtag ctcgtactga ggatatacac cccgtaattc
     1021 agagtgaaaa gggctctcgg gtcaatgtga gcgtccatgg ttgacctccc ttggataaaa
     1081 aatgagcagg aaccctgggt tcctgctcaa tgatacacca gatccgcatt tcggggaaag
     1141 ccgggaaacc ggcctaatca gcctttctcc ccccgagttc cttgtccagc atgtatatgc
     1201 cgtgcctgcc ttccccgatc atctcaagct ttttgacgat ctcgtcggca ttggcctctt
     1261 cctcgacctg ttcactgacg aaccactgga gaaagacctg gcttgcatag tctttttcgg
     1321 ccacggaaag atccatgaga tcattgatcc gcctggtgac ataccgctca tgctcataag
     1381 cgcccttgaa cacttcgaga gcggaagccc attcctcctg gggacattcg atggccttgt
     1441 acttaacccg tccgccccgc tcaacgatat aggaagcaaa cttcatggca tgttccactt
     1501 cctcttctgc ctggaccttc atccagtggg ccatgccgtt caggtccgcc gagttcaggt
     1561 aggcggacat ggagagataa atataggcag aatacagttc agcgtttatc tggtcgttga
     1621 acgcatcttc aattttcttg cttatcattc ttagagatcc accttccata aaccgtgaat
     1681 attgcaatat tccctgctct tcgtgggagt aaaggagtcg aagacagcct cggcagcctc
     1741 tcccggattg aggaattttc tgcagattct ctctccgtcc atgatctcga tgaactcgat
     1801 gtaatgttcg tttgtcatgg ggtgaggaac gctgcccacc ttgaccttgt ttccctcgac
     1861 gaccggaaca tgcttctcag tggccgaatc ggctgatttt tcctcgagga gcttcatcgg
     1921 ctgtccgcag cacacaaggt ctccccctcc tacatgaagg agttcaacaa cattgccgca
     1981 caggtcgcat ttgaacacat ccagcttctt catcccgttt ctttcctcct tttgacatga
     2041 aatgacgttg ttgtttgtaa acggattcat tatagcatta atttgttctt ttcccaaaga
     2101 gaccgatagg ttttcccttc agagggacgg accgccttca ttcaatgcgt ccgtccgccg
     2161 atacatatag cggatatcct gccaagaacg tcctggaggc tgaaaccatg aaaccgacaa
     2221 tgaaaacgaa cgctgttgcc cttgtgtttt ccctgtgttt tctgttttcg gcagaagcgg
     2281 gtgtatcggg tagaaaaacg gtgctcctcc ttcccacgct gaatttcaca gaatatgaaa
     2341 tttgggaaag caaattttac cccgtcaatg tcctcgaacg gaaaatgaat gaacacctcg
     2401 cctcccttct gcgaaggg
//
LOCUS       DAOTHD010000282         2411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_52033_length_2411_cov_4.086587, whole genome shotgun sequence.
ACCESSION   DAOTHD010000282 DAOTHD010000000
VERSION     DAOTHD010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52033_length_2411_cov_4.086587"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1026
                     /locus_tag="P5219_05700"
     CDS             124..1026
                     /locus_tag="P5219_05700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRX26283.1"
                     /translation="MERNNEARRITFLGLVVNIFLTAAKYLAGYFGRSAAMIADATHS
                     LSDLLTDLVVYFGLSAAGKPADSRHPYGHGKIEAVLSAVCGISLLLAAGGIFFSGGAR
                     IWSLLFRGAETAGPGIIALAAAGVSVVLKEWLFRYTIAGGRRLQSSALIAKAWDHRSD
                     ALSSVGTLAGIAGAFFGGERWSVLDPLAALVVSLFIVTAALPILRDSLDELIEAALPG
                     ELEKKLQSVIVSVPGVRSFHKLKTRRIGSSIAVDVHIQMNGTMSLNEAHEISRKVEHE
                     IWALFGHDAHISLHMEPASPAAGR"
     gene            1117..>2411
                     /locus_tag="P5219_05705"
     CDS             1117..>2411
                     /locus_tag="P5219_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HRX26284.1"
                     /translation="METQSAKGPHFIEQIIQDDLKNGVVKEIRTRFPPEPNGYLHIGH
                     AKSICLNFGLAEQFGGTCNLRFDDTNPAKEETEYVESIMRDVQWLGFRWHRLCFASDY
                     FHRFHEWALELIHAGKAYVDHQSADEIRETRGTLTAPGKESPYRNRTVEENLALFESM
                     KRGELENGTCVLRAKIDMGHPNLNMRDPVLYRILHRHHFRSGGEWCIYPMYDFAHGYE
                     DAIEGVTHSICTLEFEDHRPLYEWLLDNVSVPCKPRQFEFARLNLTYTVMSKRLLLTL
                     VNEKIVSGWDDPRMPTISGIRRRGYTPSSLRRFCQEIGVSKSNSMVDIEFLQYIIREE
                     LNLEARRAMAVLDPLKVVIENYPEGQTEMLEAENNPEKPEDGTRQIPFSREVYIERDD
                     FMENPPKKFFRLSPGTEVRLKHAYLITCTEVVRNGDGEIT"
BASE COUNT          516 a          690 c          703 g          502 t
ORIGIN      
        1 agacttcagg aggtcatttc cgtcgctcca tcacaggacc atcggcattc ttgacttgat
       61 ggccgaaagg ctcataatga cgggaaaccg ccgatgaccg ggcgaaatga aagaaggatg
      121 gagatggagc ggaacaacga agccaggagg ataaccttcc tggggcttgt ggtgaatatt
      181 tttctgacgg ccgcgaaata tcttgccggc tatttcggcc ggagcgcagc aatgatcgcc
      241 gatgccaccc attccctgtc cgacctgctg accgatctcg tagtgtactt cgggctctcg
      301 gcggctggga agcctgccga ttcccgccac ccctacggtc acggcaagat cgaagccgtc
      361 ctttcggccg tgtgcggaat ttcccttctc cttgcggcgg gggggatctt cttctcggga
      421 ggggctcgga tatggtccct gctgttcagg ggcgcggaaa cggccggtcc cggcataatc
      481 gccctggcag cggcgggagt ttccgtggtg ctgaaggagt ggctcttccg gtacaccatc
      541 gcgggaggac ggcgtctcca gagcagcgcc ctgatcgcca aggcatggga tcatcgctcc
      601 gatgccctct catccgtcgg tacccttgcg ggaatcgccg gtgccttttt cggcggcgag
      661 cggtggagtg ttctcgatcc cctcgctgcc ctggtggtga gcctcttcat cgtgactgcc
      721 gcgctgccca tactgaggga cagtcttgac gaacttatcg aggctgccct tcccggggag
      781 ctggaaaaga aactccagtc ggtcattgtt tccgtgccgg gggttcgatc ctttcacaaa
      841 ctgaagaccc ggcggatagg aagctccatc gccgtggacg tgcatataca gatgaacggg
      901 accatgtccc tcaacgaagc ccatgaaatt tcccggaagg ttgagcatga gatctgggca
      961 cttttcggcc atgacgccca tatttctctc cacatggaac cggcctcgcc ggcggcgggc
     1021 cggtgagttt tacaatttgc cttctccgtg ccaaaataga acggcacaac aggtgttcat
     1081 tccattttcc gccaagcaaa ggagcgtttt gaattgatgg aaacccagtc cgcaaaaggc
     1141 cctcatttta ttgaacagat catccaggac gacctgaaaa acggcgtggt gaaggaaatc
     1201 cgcacccgtt ttcctccgga gccgaacggg tatctgcata tcggtcacgc caagtccatc
     1261 tgcctgaact tcggccttgc ggagcagttc ggggggacgt gcaatctccg cttcgacgat
     1321 acgaacccgg ccaaggaaga gacagaatac gtggagtcca tcatgcgtga cgtccagtgg
     1381 ctcggtttcc ggtggcacag actgtgtttc gcctccgatt attttcaccg gttccatgag
     1441 tgggccctcg agctgattca tgccgggaag gcctacgtgg accaccagtc cgcggacgaa
     1501 atccgggaga cccggggaac cctcacggct cccggcaaag agtctcccta caggaaccgt
     1561 accgtggaag aaaaccttgc ccttttcgaa agcatgaaaa ggggagaact cgagaatggc
     1621 acctgcgtcc tccgggcgaa gatcgacatg ggccacccca acctgaacat gagggatccg
     1681 gtgctgtacc gcattcttca caggcaccac ttccggtccg gtggagagtg gtgcatctac
     1741 cccatgtatg actttgccca cggctacgag gatgccatcg agggcgttac ccattccatc
     1801 tgtaccctgg agttcgagga tcacaggccc ctgtacgaat ggctgctgga caatgtgtcc
     1861 gttccctgta agccgaggca gttcgagttc gcccggctga accttaccta tacggtgatg
     1921 agcaagcgtc ttctgctcac tctggtcaac gagaaaatcg tctccggctg ggatgacccg
     1981 agaatgccca ccatcagcgg catccgtcgc agggggtaca ccccgtcgtc tctccgcagg
     2041 ttctgccagg agatcggcgt gtcaaagtcc aacagcatgg tggacatcga gtttctgcag
     2101 tacatcatcc gggaagagtt gaacctggag gcccggcggg ccatggcagt ccttgatccg
     2161 ctgaaggtgg tcatcgagaa ctaccccgaa gggcaaactg aaatgctcga ggcggagaac
     2221 aacccggaga aaccggaaga cgggacacgg cagattcctt tctccaggga agtttacatt
     2281 gagcgggacg atttcatgga aaatccgccg aagaaatttt tccgtctttc tcctgggacg
     2341 gaagtgcggc tgaagcatgc ctacctgatc acctgcaccg aagttgtcag gaacggggat
     2401 ggagagatca c
//
LOCUS       DAOTHD010000283         2402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_52315_length_2402_cov_3.272689, whole genome shotgun sequence.
ACCESSION   DAOTHD010000283 DAOTHD010000000
VERSION     DAOTHD010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52315_length_2402_cov_3.272689"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="P5219_05710"
     CDS             complement(<1..334)
                     /locus_tag="P5219_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRX26285.1"
                     /translation="MTRIIGLQDIKNAAGRIAGKVRRTPILRGDKLDALWGAEVYFKP
                     ENLQLTGSFKIRGATNKILSLTDEERAKGIIASSSGNHAQGVAYAAKMLGIKATLVLP
                     ENAPKSKIE"
     gene            complement(436..636)
                     /locus_tag="P5219_05715"
     CDS             complement(436..636)
                     /locus_tag="P5219_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRX26286.1"
                     /translation="MAVHVNKRLCKGCGLCISVCPKNVYEISSETNQKGFTVVAAVRE
                     SDCIKCRRCEITCPDMALWIEE"
     gene            complement(644..1510)
                     /gene="sucD"
                     /locus_tag="P5219_05720"
     CDS             complement(644..1510)
                     /gene="sucD"
                     /locus_tag="P5219_05720"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HRX26287.1"
                     /translation="MAILVDRDTRVLVQGITGKSGILQTKSLIDYGTAVVAGVTPGKA
                     GTAVEGVPVFNSVADAVREVSPNAAISFVPPLFAKDAAMEAMEAGIKLLVLTMEGIPK
                     HDVLDILSYASGRGVRVLGPGTAGVISPGKCKLGAHPARMFREGKVGVVSKSGALSYE
                     VGKTLTDAGIGQSTVVALGGGPIWGTTQRDIVKLFNEDPETEIIVLLGEIGGGTEIAA
                     AEYIAESVRKPVVSLIVGRAAPEGKSLGHAGAIIRGNKGTAASKMEALEKAGACLATS
                     PAQVVELIRKLG"
     gene            complement(1512..>2402)
                     /locus_tag="P5219_05725"
     CDS             complement(1512..>2402)
                     /locus_tag="P5219_05725"
                     /inference="COORDINATES: protein motif:HMM:NF020031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26288.1"
                     /translation="PVRALLVEEKMNIRAEYYLAVTFDGEAGTPLFMASASGGMDIES
                     VAEHSPEKILKLPVDPLWGLTDYKLRAMADFLGYENKKEFASFARKVWTLFREKNAVL
                     VEINPLVVTDSGLVALDSKIEIDDDARPRLKDLFEKNLAEQAALTGVEGEADHGTITY
                     VPLEGNVGLISDGAGTGMLTLDLIRDLGGEAADFCEMGGLTSPEVMYSAMDQVFSDKK
                     DIKSLLVVLIGGFNRMDEMAEGITAYLREHKVSIPLVVRLCGTMEEEGKAIMKEAGLP
                     VYDDLRTAVADAVRFAAGGN"
BASE COUNT          402 a          774 c          729 g          497 t
ORIGIN      
        1 cctcgatctt cgacttgggg gcattctcgg gaaggacgag ggtggccttg atgccgagca
       61 tcttcgccgc gtaggccacg ccctgggcgt ggttcccgga ggacgaggcg atgatgccct
      121 tcgcccgctc ctcgtcggtg agggagagga tcttgttggt ggctccacgg atcttgaagg
      181 atcccgtaag ctggagattc tccggcttga agtagacctc cgcgccccac agggcgtcaa
      241 gcttgtctcc ccgcaggatg ggggtccgcc gcaccttgcc ggcgattctc ccggccgcgt
      301 tttttatgtc ctgaagtccg ataatccgtg tcattgtact gcttcagcct ccttgtgccc
      361 ttttcgggcg ttcattgtca acgggacagg ggcggccgga atttcttccg ccgccccgcc
      421 gcccgtccgc ttattctatt cctcgatcca cagcgccatg tcggggcagg tgatctcgca
      481 ccgtctgcac ttgatgcagt ccgactcccg gactgccgcg acgacggtga accccttctg
      541 gttcgtctcg gaagaaattt cgtacacgtt cttcgggcag acggaaatgc acaggccgca
      601 tcccttgcag agccgcttgt ttacatgcac agccatgatc tcatcacccc agcttccgga
      661 ttaattccac cacctgggcg ggagacgtag ccagacaggc tcccgctttt tccagggctt
      721 ccattttcga cgccgcagtt cccttgtttc cccggatgat cgcaccggcg tggcccaggg
      781 attttccctc cggagccgcc cggcccacga tcagggagac cacgggcttg cggaccgatt
      841 cggcgatgta ttccgccgcg gcaatctcgg ttcctccgcc gatttcgccg aggagaacga
      901 tgatctccgt ctcgggatcc tcgttgaaga gtttcacgat gtcccgctgg gtggttcccc
      961 agatggggcc gccgccgaga gcgacaacgg tggactggcc gatgccggcg tcggtgaggg
     1021 tcttccccac ctcgtaggac agggcgccgc tcttcgacac cacgccgacc ttcccctccc
     1081 tgaacatccg ggcgggatgg gcgcccagct tgcacttgcc cggggagatg acccctgcgg
     1141 ttcctggacc gagaacccgc actcccctgc cggaggcata ggagaggatg tccagcacgt
     1201 catgcttcgg aatcccttcc atggtgagga cgagaagttt gattccggcc tccatggcct
     1261 ccatggcggc atccttggcg aacaggggag gaacgaagct gatggccgcg ttgggagaca
     1321 cttccctgac ggcgtccgcc acggagttga acacgggaac accctccacg gccgtgccgg
     1381 cctttcccgg ggtgacaccg gcgaccaccg ccgtgccgta gtcaatcagg gatttggtct
     1441 ggagaatgcc cgattttccg gtgattccct ggacgagaac ccgggtatcg cgatccacaa
     1501 gtatggccat gtcagttccc tcccgcggcg aaccgcacgg cgtcggcgac ggcggtccga
     1561 agatcgtcat agacgggaag gcccgcctct ttcataatgg ccttgccctc ttcctccatg
     1621 gtgccgcaga ggcgcaccac caggggaatg gagaccttgt gctcccgaag ataggccgtt
     1681 attccctcgg ccatttcgtc catgcggttg aagccgccga tgaggaccac gagcaggctc
     1741 ttgatgtctt tcttgtccga gaagacctgg tccatggcgg agtacatcac ttcggggctc
     1801 gtcaggccgc ccatttcgca gaagtccgcc gcctcgccgc cgaggtcccg gatgaggtcg
     1861 agggtgagca tccctgtgcc tgcaccgtca gaaatgaggc ccacgttccc ctcgagggga
     1921 acgtaggtga tggtgccgtg gtccgcctcc ccttccactc cggtgagagc cgcctgctcg
     1981 gcgagatttt tttcgaagag gtccttcagc ctgggcctgg cgtcgtcgtc gatctcgatc
     2041 ttcgagtcga gggccacgag gcccgaatcg gtgaccacca gggggttgat ctccaccagg
     2101 acggcatttt tttccctgaa gagggtccag accttccggg cgaaggaggc gaactccttc
     2161 ttgttctcat agccgaggaa atcggccatg gcgcgcagct tgtagtccgt gaggccccac
     2221 aggggatcca cgggaagctt gagaatcttc tcggggctgt gctccgccac cgactcgata
     2281 tccattccgc cggaggcgct ggccatgaac aggggcgtgc cggcctcacc gtcgaaagtg
     2341 acggcgaggt agtattccgc ccggatgttc atcttctcct ccacgagaag agcccggacc
     2401 gg
//
LOCUS       DAOTHD010000284         2401 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_52335_length_2401_cov_3.428389, whole genome shotgun sequence.
ACCESSION   DAOTHD010000284 DAOTHD010000000
VERSION     DAOTHD010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52335_length_2401_cov_3.428389"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="P5219_05730"
     CDS             <1..997
                     /locus_tag="P5219_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRX26289.1"
                     /translation="GRVFGLSYHALFCRGLSSYLKRGVPLAEALKFLGRHSSDRRVAD
                     ACLHLHEGVQGGRRLSAVLEETGLFREDLLKIVESGEKTSALPGVLEQAAALYSMQEG
                     QRRKIRSALTYPLAMTAIGFAVVLFLLTYVVPRLADLFADMGQALPLPTRILLGTAAF
                     LREWWPALVLVLASAVFLARRRKWSVNLPFFRRIRENIALSLVMSHLKTLLSAGIPLV
                     QALGMASSMDSRPERWVEAARLVKEGFRFDRALERLGTFPEETVYILRIGELGGDLPG
                     AIGQVAEMNWEEARDGMEKAATLAEPLLVLFLGLAVGFVVIAVLLPIFDLSSLAG"
     gene            1030..1461
                     /gene="gspG"
                     /locus_tag="P5219_05735"
     CDS             1030..1461
                     /gene="gspG"
                     /locus_tag="P5219_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HRX26290.1"
                     /translation="MKKRQQKRKGFTLVEVLVVVVILGLLAALVVPRVVGRGEEAKRT
                     AAAVQIREIEQALEMYRLDSSLYPSTAQGIEALVSKPSIPPEPRKYREGGYLRKLPAD
                     PWGSPFVYRRPGDHGEYDLFSLGADGEEGGDGPGKDITNWE"
     regulatory      1537..1655
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            1875..>2401
                     /locus_tag="P5219_05740"
     CDS             1875..>2401
                     /locus_tag="P5219_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRX26291.1"
                     /translation="MIQKVFFVNGVERNIIVEDDSTLATVLREQLGLTGTKIGCGQGH
                     CGSCGVIIDGKYTRSCVYKMSRLPEGACVTTIEGIGTPERLHPIQKAWIKHGAAQCGF
                     CTPGFIVSTKALLDTNPNPTREEVRDWFHVNKNACRCTGYIPIVDAVMDAAKVLRGEM
                     NEKDLEFVLPGDGRI"
BASE COUNT          440 a          721 c          784 g          456 t
ORIGIN      
        1 gggcagagtc ttcggcctct cctatcacgc cctgttctgc cgcggcctct cctcctacct
       61 gaagcgggga gttcccctcg ccgaagccct gaagttcctg ggccgccatt cctcggaccg
      121 gcgggtggcc gatgcctgcc tccatctcca cgaaggggtc cagggaggaa ggcgcctctc
      181 cgccgtcctg gaggagacgg ggctcttccg ggaggacctc ctgaagatcg tggaatcagg
      241 ggaaaagacg tcggcccttc ccggcgtgct ggagcaggcc gcagcgctgt attccatgca
      301 ggaggggcag cggcggaaga tccggtccgc cctcacctac cccctggcca tgacggccat
      361 aggcttcgcc gtggtcctct ttctgctcac ctacgtggtg cccaggctcg ccgacctgtt
      421 cgccgacatg ggacaggccc ttccccttcc aacgaggatc ctcctcggga cggcggcgtt
      481 cctccgggag tggtggccgg cgctggtcct cgtcctcgcg tcagccgttt tcctggcgcg
      541 gaggagaaaa tggtccgtga acctcccctt tttccgccgc atccgggaga acatcgccct
      601 ttccctggtg atgtcccacc tgaagaccct gctctcggcg ggaattccgc tggtccaggc
      661 ccttggaatg gcctcttcca tggactcccg gccggagcgg tgggtggagg cggcccggct
      721 cgtgaaggag gggttccggt tcgaccgggc cctggagcgc ctggggacct ttcccgagga
      781 gacggtgtac atcctcagga tcggcgagct gggcggcgac ctgcccggag ccatcggcca
      841 ggtggcggag atgaactggg aggaggcccg ggacggcatg gaaaaggccg ccacccttgc
      901 cgaacccctt ctggtgctct tccttggcct ggcggtgggc ttcgtggtca tcgccgtcct
      961 gttgcccata ttcgatctgt caagcctcgc ggggtgacgg gcggacagaa ctgaaacgga
     1021 ggttgtgcaa tgaagaaaag gcagcagaaa cggaaaggct ttacgctggt ggaagtgctc
     1081 gtggtggtgg tcatcctcgg cctgctggcc gcccttgtgg tgcccagagt ggtgggccgg
     1141 ggcgaggagg cgaagaggac cgcggcggcg gtgcagatcc gggagatcga acaggccctc
     1201 gagatgtacc ggctggacag cagcctctat ccatccacgg cccaggggat tgaagccctg
     1261 gtgtcaaagc cctccattcc tcccgagccg aggaaataca gggagggagg atacctgcgg
     1321 aagctcccgg cggatccgtg ggggagtccc ttcgtctacc ggcgtcccgg cgaccacggt
     1381 gaatacgacc tgttctccct gggcgccgac ggcgaggaag gcggggacgg accggggaag
     1441 gatataacca actgggaata gacttgccgg gaaactcttc ctggtataaa atggctcaaa
     1501 ctagttagtg tttttttgaa atttacttct gagaatatca atcttttcct ccgagttgcc
     1561 tgccctaaag gaaaaatccc acggaaggcg accgttaggg tgcgcccgga aacaggcgtg
     1621 cctccccttt ggcaaggagg acgttttttc cgcacacggc gaagccgtcc tcatgtcttg
     1681 agggcggctt ttatttttta tcagcacgag gtgaaaggag gggtcggttc agcggattac
     1741 tgacgaagat cccgcgcgaa gtctttgttg aacaggaaag gggcggcggt tccggtcccc
     1801 atcgcctggc cgcgttcagg tccggagttc tgccgccctg cgcctgccga agaacggaag
     1861 gagctgggaa aaggatgatt cagaaagtct tttttgtcaa cggcgtcgaa cggaacatca
     1921 tcgtggagga tgacagcacc cttgccaccg tgctcaggga gcagctcggg ctgacgggga
     1981 ccaagatagg atgcggccag ggccattgcg gttcctgcgg cgtcatcatc gacggcaagt
     2041 atacccggtc ctgcgtgtac aagatgtcga ggctgcctga aggagcctgc gtcaccacca
     2101 tcgagggcat cggtaccccc gaacgtctcc atccgatcca gaaggcctgg atcaagcacg
     2161 gggccgccca gtgcggcttc tgcaccccgg ggttcatcgt gtcgacgaaa gcccttctcg
     2221 acaccaaccc caacccgacc agggaagaag tccgggactg gttccacgtt aacaagaacg
     2281 cctgccgctg tacggggtac attcccattg tcgatgccgt catggatgcc gccaaggtgc
     2341 tccggggcga aatgaacgag aaggatctgg aattcgtcct tcccggggac gggcggatct
     2401 g
//
LOCUS       DAOTHD010000285         2396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_52489_length_2396_cov_3.434857, whole genome shotgun sequence.
ACCESSION   DAOTHD010000285 DAOTHD010000000
VERSION     DAOTHD010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52489_length_2396_cov_3.434857"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..1395
                     /gene="truD"
                     /locus_tag="P5219_05745"
     CDS             235..1395
                     /gene="truD"
                     /locus_tag="P5219_05745"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HRX26292.1"
                     /translation="MKIKVNPEDFQVEEVLSVPLLEKKTSYRVYRLRKRNWNTVDALA
                     DIARASGVSLKELHWCGRKDRSALTTQYFSTTSQADMTFRSTNVSTEFAGFADSELGP
                     SSIAGNRFRVVLRDMVPEEAGAVSNRLESLRKHGFANYFDDQRFGDVENGGFIGEMLV
                     KRRFKAAVKRIFTACHSEAPAETRKRKKALADRWGRWSDVATLCPEPQLKAMLEALMS
                     GGGPIGSLREVPREEMGQYFSSYQSFIWNLVLAEVIKNRECRLFEIPCRTGSLPQYDE
                     LSTDDLAFLSELQIPTAAADMPPMPGETGAILSRILFERGVRLSKFRLPEIPQAYFAS
                     FARPAVVVPGEMKWEEAPDGLFSGKLMLTLGFFLPRGSFATMLIKSISGNPE"
     gene            1420..2355
                     /locus_tag="P5219_05750"
     CDS             1420..2355
                     /locus_tag="P5219_05750"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HRX26293.1"
                     /translation="MKKVPALREGDTVAFVSPSSQSTLESVEKAVSVAEDMGFHVKVA
                     KSCYCSEGYHAGPDALRADDVNRMFLDPEVKGIFCVRGGDGATRLPPLIDIEGAFTNP
                     KVFLGYSDITILHQMFNRPGGFCTFHGPMPASEFTKDTFPGYVRDSLLRAVCSTEPLG
                     EIKPYEGAAPMETLVPGNAEGELIGGNLALVCALLGTPWEIDTRGRILVLEDTGEPVY
                     RIDRMLTQLRLAGKLDAAAGIVLGQFTQIQPRKPKDSFTVPEVFEAVVAPVGRPTLLN
                     GCFGHDVKKATLPLGARALIDGAASTLTVVEAGVY"
BASE COUNT          508 a          673 c          738 g          477 t
ORIGIN      
        1 cgtgcatgtc tacacgagac cggggtcgaa gagtcccctt gggttgtttt cgaccccggt
       61 ctcgtgtaga catgcacgag accggggtcg aagagtcgcc ttgggttgtt ttcgcgcatc
      121 attatctgct cctccttccg gtgtctgaaa ccgtggtata tgggaatgcc ggaaaatacg
      181 tacaatggtt tatccttgtt ccatggatca aaatcgcgcg ctggaggaat actgttgaag
      241 atcaaggtca atcccgagga tttccaggtg gaagaggtgc tgtcggtccc cctgctggag
      301 aagaaaacct cttaccgagt ataccgcctt cgcaaaagga actggaacac ggtggacgcc
      361 cttgcggata tcgcccgggc gagcggagta tctctgaagg agctgcactg gtgcggcagg
      421 aaggaccgct ccgcactgac gacgcagtac ttctcgacga cctctcaggc ggacatgacc
      481 ttcagaagca ccaacgtttc gaccgaattc gcgggctttg cagactcgga gctgggaccc
      541 tcttccatag cgggcaaccg gttcagggtc gttctgaggg acatggtccc tgaggaggcg
      601 ggagccgttt caaacaggct cgaatctctg aggaagcatg gctttgcgaa ctatttcgac
      661 gaccagcgct tcggagacgt cgaaaacggg ggatttatcg gcgaaatgct cgtcaagcgg
      721 cgtttcaaag ccgccgtaaa aaggatattc actgcctgcc actccgaggc ccctgcggag
      781 acgcgaaagc gcaaaaaagc cctggcggac aggtggggaa gatggagtga cgtggcgacg
      841 ttatgtccgg aaccgcaact aaaggcgatg ctggaagccc tgatgagcgg cgggggcccc
      901 atcgggtcgc tcagggaagt gccgcgtgag gagatgggac agtacttctc atcctaccag
      961 agctttatct ggaacctggt acttgcggaa gtgataaaaa accgggagtg caggcttttc
     1021 gagatcccat gccggacggg atcactcccc cagtacgatg aactctccac tgacgatctg
     1081 gcatttcttt cggagctgca gatcccgacg gcagccgccg acatgccgcc aatgccgggg
     1141 gagacaggag caatactctc ccgcatcctg ttcgaaaggg gcgtgaggct ttcaaagttc
     1201 cggctgccgg agatccccca ggcttatttc gcatcctttg cccgtccggc ggtggtcgtg
     1261 cccggtgaga tgaagtggga agaggccccc gacgggctct tttccggaaa gcttatgctg
     1321 accctcgggt tctttctccc caggggctcc ttcgcgacga tgctgataaa atccataagc
     1381 gggaatcccg aataaaagaa gagcggggga gtgatttcca tgaaaaaggt accggcactt
     1441 cgcgaagggg acacggtggc cttcgtctcc ccttcgagcc agtcgaccct tgaatcggtc
     1501 gaaaaagccg tatctgtcgc ggaggacatg ggtttccatg tcaaggtggc caaaagctgc
     1561 tactgctccg aggggtatca tgcgggcccg gacgctctgc gcgccgatga cgtgaacagg
     1621 atgttcctgg acccggaggt gaaggggata ttctgcgtcc ggggcgggga cggggccaca
     1681 aggcttccgc cccttatcga tattgaaggt gccttcacaa accccaaggt ctttctgggc
     1741 tacagcgaca taacgatcct tcaccagatg tttaaccgcc ccggcggctt ctgcaccttt
     1801 cacggcccga tgcccgccag cgaattcaca aaagacacct tccccggcta cgtgcgggac
     1861 agcctcctca gggccgtctg ctcaacggag ccgttgggtg agataaaacc ctacgaagga
     1921 gccgcaccga tggagaccct cgttccgggt aatgccgaag gcgaactcat cggtgggaac
     1981 ctggcactgg tatgtgccct gctggggact ccctgggaga tcgacacgcg ggggcgcata
     2041 ctggttctcg aggatacggg cgaaccggtc taccgcatcg acaggatgct cacccagctt
     2101 cgcctggcgg gcaagctgga cgccgcggca gggatcgtcc tgggtcagtt cacccagatc
     2161 cagccgagaa aaccgaagga ttccttcacg gtccccgagg tattcgaggc ggtcgtggcc
     2221 cctgtcggca ggccaaccct tttgaacggc tgtttcggcc atgacgtgaa gaaggcgacc
     2281 cttcccctcg gtgccagggc cttgatcgac ggcgctgcat caaccctgac ggtcgtggaa
     2341 gcgggggtgt attaggtaac gcgtgactcg ttacgcgtga ctcgtgactg gtaaga
//
LOCUS       DAOTHD010000286         2390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_52662_length_2390_cov_1.994861, whole genome shotgun sequence.
ACCESSION   DAOTHD010000286 DAOTHD010000000
VERSION     DAOTHD010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52662_length_2390_cov_1.994861"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..469
                     /locus_tag="P5219_05755"
                     /pseudo
     CDS             1..469
                     /locus_tag="P5219_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869438.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
     gene            481..1371
                     /locus_tag="P5219_05760"
     CDS             481..1371
                     /locus_tag="P5219_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRX26294.1"
                     /translation="MSGYVEGIIILLFINGIAAMGVSLLTGFTGVFTLGHAGYMAIGA
                     YTAAIMTVRFGIHWLPAILAGGVIAMVLAYLIGVPTLRLMGDYYAIASIGLGESIRLI
                     LENWQSFTRGARGYPGIDNYTNLSVAAAFFVVMALLMFFLIRSNFGRAFKACRDDYVA
                     ASLLGFNTARYRVLSLSISAFYCGVAGALMAGFLTFIQPVMFDLNKSTELTAVVVFGG
                     LGSLSGSLIGTAVITLVTELFRPISQYRMLIYGAVMVIIMVVRPEGIMGQHELGPRFI
                     KSLFRRKGREPKAVEGSDGL"
     gene            1368..>1530
                     /locus_tag="P5219_05765"
     CDS             1368..>1530
                     /locus_tag="P5219_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRX26295.1"
                     /translation="MSETVLKLDHVNKYFGGVHAVQDMSFSLEKGELAGLIGPNGAGK
                     TTIFNLITGV"
     assembly_gap    1531..1540
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1541..2166
                     /locus_tag="P5219_05770"
     CDS             <1541..2166
                     /locus_tag="P5219_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX26296.1"
                     /translation="GVYDVDSGSILFEGEDITPYKTYEVIRKGIARTFQNLRLFPRST
                     VLENVMTACQQHYGYNFLEAASHLGRWKSMEKEIRQQSMTLLERVGLDSLAEQAAGTL
                     PYGHQRRLEIARALSLQPRLLLLDEPAAGMNPDEVQDLNGLITSIHRDFGLTILVIEH
                     HMDLVMEICPHIVCMNFGAKIAEGDPDHIQNHPEILKAYLGEEVLEA"
     gene            2163..>2390
                     /locus_tag="P5219_05775"
     CDS             2163..>2390
                     /locus_tag="P5219_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRX26297.1"
                     /translation="MIDSQDKKQPLLSVRELHVSYGAIKAVRGFNLDVYPGEIVCVIG
                     SNGAGKSTLMNAVMGNVKRQSGQVLFEGKPLE"
BASE COUNT          483 a          678 c          664 g          555 t
ORIGIN      
        1 gacgacaagc gcgttgggcc tgctctcggt gtgtatggcc gtgtagttcc tcaccgggct
       61 gcagctcttc ctcagcaaga cgaaatgggg gcttgccata cgggccgcat catacgacct
      121 ccgcacatcc ggcttgatgg gggtcaacgt gaatcgcctt atctcgatcg ttttcttcgt
      181 ggccggcttt ctcgcggctg taggcggcat ctttctctcg gtccgttaca cgctttatcc
      241 acagctgggc ttcatcacga taaaggcttt tgttgccgcc gtcatcggcg gactgggaag
      301 ccttccagga gccgtactcg gcagcctgat tctcggactg gcggaaatgc ttacggcggg
      361 attcatctca agccagatgc gggatctggt cgtgttcggc atgctggtcc tgacgcttct
      421 ctggaggcct tccggccttc tgggcaagca agtcagtgaa aaggtatagg gggcttttcc
      481 atgtctggat acgttgaagg gatcattatc ctccttttca tcaacggcat cgccgccatg
      541 ggagtttccc tgctgacggg cttcacggga gtgttcaccc ttggtcatgc cgggtacatg
      601 gcgatcggcg cctacaccgc tgccatcatg acggtcaggt tcggcattca ctggcttccc
      661 gcgatcctgg ccggtggcgt tatcgccatg gtgctcgcct acctcatagg cgtcccgacc
      721 ctacgcctga tgggcgatta ttatgccatc gcttccatag gactgggaga atccatccgc
      781 ctaatcctgg aaaactggca atccttcacc cgcggcgcaa gaggataccc agggatagat
      841 aactatacaa acctctcggt tgccgcagcg tttttcgtcg tcatggcgct actgatgttc
      901 ttcctgatca ggagcaattt cgggagggcg ttcaaggctt gccgggacga ctatgtggcg
      961 gcttcccttc tgggtttcaa tacggcccgc taccgtgtcc tgagcctttc gatctcagct
     1021 ttctactgtg gcgtggcagg tgctctcatg gcgggtttcc tgacctttat ccagccggtc
     1081 atgttcgacc tcaacaagtc taccgagctt accgcggtcg tcgtattcgg gggcctgggc
     1141 tccttgagcg gaagccttat cggcactgcc gtgatcacgt tggtgacgga acttttccgg
     1201 cctatctccc agtataggat gctgatttac ggtgctgtca tggtcatcat catggtcgtc
     1261 cgcccggagg ggatcatggg ccagcacgag ctaggccccc gcttcatcaa atccctcttc
     1321 aggaggaagg gtcgtgagcc aaaagcagtg gaagggagtg acgggctatg agcgaaaccg
     1381 ttctcaagct tgatcacgtc aacaagtact tcggtggagt ccacgccgtc caggatatga
     1441 gtttctcgct tgagaagggc gagctcgcag gcctgatcgg ccccaatgga gccgggaaga
     1501 ccacgatatt caacctgatt acgggtgttt nnnnnnnnnn cgggtgttta cgatgtagac
     1561 agcggcagta tccttttcga aggcgaggac atcacgccct acaagaccta cgaggttata
     1621 cgcaagggaa tcgcccgaac cttccagaac ctgcggcttt tccccaggtc gaccgttctc
     1681 gaaaacgtca tgaccgcctg ccagcaacat tatgggtaca actttttaga ggctgcctcc
     1741 cacttgggcc gctggaaatc catggaaaag gaaattcgcc agcaaagcat gacccttttg
     1801 gaacgagtgg ggcttgattc acttgccgaa caggcagcag gcacacttcc ttacggacac
     1861 cagcgccgcc ttgaaattgc aagggctctc tccctgcagc ctcggcttct tctcctcgac
     1921 gaacccgccg caggaatgaa cccggacgag gtccaggact tgaacggcct gatcacgtcc
     1981 attcaccgcg atttcgggct gaccatcctg gtcatcgagc accacatgga cctggtcatg
     2041 gagatttgcc cacatatcgt ttgcatgaat ttcggcgcca agatcgctga gggtgatcct
     2101 gatcatatcc agaaccaccc ggaaatcctg aaggcctacc tcggcgagga ggtgttggaa
     2161 gcatgatcga ttcccaggac aagaaacaac cgctcctttc cgtaagggaa ctgcacgtct
     2221 cctacggcgc catcaaggct gtccgtgggt tcaaccttga cgtttacccg ggagaaatcg
     2281 tctgcgtcat tggatcaaac ggcgcgggaa aatcgacact tatgaatgcg gtcatgggta
     2341 acgtgaaaag acagagcggg caagtccttt tcgaagggaa acccctcgag
//
LOCUS       DAOTHD010000287         2380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_52942_length_2380_cov_3.735914, whole genome shotgun sequence.
ACCESSION   DAOTHD010000287 DAOTHD010000000
VERSION     DAOTHD010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52942_length_2380_cov_3.735914"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="P5219_05780"
     CDS             <1..696
                     /locus_tag="P5219_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26298.1"
                     /translation="GKAKLKKLHLYSAPVQDISVLAELTELEDLSLYMIKADDLSPIS
                     GLTKLKRLSLYAIPATDMSPVENLTELRYIWIYATKFEDYSPLAKLTKLEEFHGGLSK
                     LDTLEYVESMPKLKVVKMLREDIIDVSPLAKCPDLKEAVLESITGPVDLAVFAGHKKL
                     EALDVEGCKVLNPEAIAALPRLQRLDLQKTEGVADFSIFKDLPKLQYIYAKVGQFPDE
                     QIAPYGKKAIIQK"
     gene            753..1043
                     /locus_tag="P5219_05785"
     CDS             753..1043
                     /locus_tag="P5219_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRX26299.1"
                     /translation="MTEYLPEPLGKKTGVRGGKRKGPSRAEMSIRLQLAPFSLTRKET
                     EITLLLLEGLKREEILENCEITNNTLKTHIRQIYRKLNVTARADIPGKIGLA"
     gene            1150..>2380
                     /locus_tag="P5219_05790"
     CDS             1150..>2380
                     /locus_tag="P5219_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26300.1"
                     /translation="MKRRLSALFLSLLLVAALVAPGWSGTLDALPQWPDSAMYGVVNL
                     EKPNEIVQRVVNSYLFKTITALDPGIKMAEEWLKQFPVTSASVVFGLGEKGFSMQGAI
                     RFTDAKKDILARMAEGKGQEGDVDALINSPVPGELILAPMEGSTYAVVSGGGVAVALV
                     SVEGDMILVGFTPEDLAAARDALKDGGRRMKLDRRLPQGNFFFFHDNGMAASELQAES
                     DGVLKPPVGNLLAEAGINAADTGYDFSVFTNFAKVFSLLGEEKSGPMAPGDRILVGGG
                     KPWFVFMGKVFLDKKHFQAVRDAAAAGDSDAAEFVKLLEDMKQFGLDEDAVLKILKNI
                     GIVLGSQTKVMGNPLPGGYAYLSGEKAEVQLLLPLLEMVVRESGMPFETIAREGWTAF
                     YALRQPVEFVMGIKDGVI"
BASE COUNT          536 a          659 c          735 g          450 t
ORIGIN      
        1 gggaaggcga aactgaagaa gctccacctg tattccgccc ctgtccagga catatccgtc
       61 ctggcggagc tcacggagct tgaggacctc agcctctaca tgatcaaggc tgacgatctt
      121 tccccgatat cggggctgac gaaactgaag aggctgagcc tgtacgcaat tccggccacg
      181 gacatgtctc ccgtggaaaa cctgacggaa ctgcgctata tctggatata cgcgaccaag
      241 ttcgaggatt attcgcccct ggcgaaactc acgaaactgg aggagttcca cggagggctt
      301 tcgaagctgg atacccttga atacgtggag tccatgccga agctcaaggt tgtgaagatg
      361 ctcagggagg acatcatcga cgtgtcgccc ctggcgaaat gtcccgatct caaggaggcc
      421 gttctcgagt ccataaccgg tcccgtggac cttgcggtct tcgcgggcca caagaagctc
      481 gaagctttgg acgtggaagg ctgcaaagtc ctcaacccgg aggcgatcgc tgctttgccc
      541 aggctgcagc gcctcgacct ccagaaaacg gagggcgtcg ccgatttctc cattttcaag
      601 gatcttccga agctgcagta catttacgcg aaggtggggc agttcccgga cgagcagatc
      661 gctccctacg ggaaaaaagc gattatccag aaatagagag gcgagggagg agacggcagc
      721 cctccctgcc cttgtgaggg ggttggatag ccatgacgga gtatcttccg gagcccctgg
      781 gaaaaaaaac gggcgtgaga ggcgggaaaa ggaaggggcc ttcacgggcc gagatgtcca
      841 ttcggctgca gctcgcccct ttcagcctga cgagaaagga gacggaaatc acgctgctcc
      901 tgctggaagg gctcaagaga gaggagattc tcgaaaactg cgagattacg aacaacacgc
      961 tcaaaactca tatccgtcag atttacagga aactgaacgt cacggccagg gcggacattc
     1021 cggggaaaat aggattggcg tgacagcggc tcaatggtgg agtaatgtag tgccaccccg
     1081 gatacgccgg ggaaaacggg cgggaagaaa acaaaagcct tcttcccgtc tcccaaggag
     1141 gagattctca tgaaaagaag gttatccgcg ctgttcctct ccctgctcct ggtggcggct
     1201 cttgttgccc cggggtggtc gggcactctc gatgcccttc cccagtggcc cgactcggcc
     1261 atgtacgggg tggtcaacct cgagaagccc aacgaaatcg tccagagggt cgtgaactcc
     1321 tacctcttca agacgatcac cgccctcgac cccgggatca agatggccga ggagtggctg
     1381 aagcagttcc ccgtcacgtc cgcttcagtg gtcttcgggc tcggcgagaa aggattctcc
     1441 atgcagggag cgatccggtt caccgacgcg aagaaggaca tccttgcccg catggcggag
     1501 ggcaagggac aggagggcga tgttgacgcc cttatcaaca gccccgttcc cggcgaactt
     1561 atccttgcgc ccatggaggg atccacgtat gccgtggtga gcggaggcgg agtggcggtc
     1621 gccctcgtgt ctgtcgaagg agacatgatc cttgtagggt tcactccgga agacctcgcc
     1681 gcagcccggg atgctctgaa ggacggcggg aggaggatga agctggaccg caggcttccc
     1741 caggggaact tcttcttctt ccacgacaac ggcatggccg cctcggagct ccaggcggag
     1801 tccgacggag tgctgaagcc gcccgtcgga aaccttctgg ccgaagcggg gatcaacgcg
     1861 gcggacacag gatatgattt ctccgtcttc acgaacttcg ccaaggtctt ctcccttctg
     1921 ggcgaagaaa aatcgggtcc catggcaccc ggtgacagga tcctcgtagg gggcggcaaa
     1981 ccctggtttg tgttcatggg gaaggtgttc ctcgacaaga agcatttcca ggcggtccgg
     2041 gacgctgcgg cggcaggaga cagcgatgcg gccgagttcg tcaagctgct ggaagacatg
     2101 aagcagttcg ggctcgacga ggacgctgtg ctgaagatcc tgaagaatat cggaatagtt
     2161 ctcggcagcc agacaaaggt catgggaaat cctcttcccg gagggtacgc ctatctctcg
     2221 ggcgagaaag ctgaagtcca gcttcttctc ccccttctgg aaatggtcgt acgcgagagc
     2281 ggcatgccct ttgagaccat tgccagggaa ggatggaccg cattttacgc ccttcgtcag
     2341 cctgtcgaat tcgtgatggg gatcaaggac ggagtcatcc
//
LOCUS       DAOTHD010000288         2378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_53006_length_2378_cov_3.497202, whole genome shotgun sequence.
ACCESSION   DAOTHD010000288 DAOTHD010000000
VERSION     DAOTHD010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53006_length_2378_cov_3.497202"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..906)
                     /locus_tag="P5219_05795"
     CDS             complement(220..906)
                     /locus_tag="P5219_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRX26301.1"
                     /translation="MKNCSCSGGENWRNCFENVPLFQSLSPDEMSEIASITEPRTFLK
                     GEFIYREGDTGGKLYILHTGGVKISRLSSDGKEQVIRVIGPGEFIGELSLFSYLPFTD
                     DAAALENSTMCFIDGGKLKLLMSKYISIAFKVMDELSKRLERAENLIKEISLNSVEER
                     LARVLLDLSGEEREIILPMTKGDFASQLGMSQETLSRKLSSFQEEGLISLKGHKRIII
                     ENREGLQKLL"
     gene            complement(893..>2378)
                     /locus_tag="P5219_05800"
     CDS             complement(893..>2378)
                     /locus_tag="P5219_05800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRX26302.1"
                     /translation="DLVVVRPGEKIAVDGTVVEGSAYINQAAITGESIPISRTVDETV
                     FSGTVIESGYLIVRADRVGEDTTFARILQMVEDAQDKKAITQKFLEKFSRYYTPAIIF
                     LSIALFLATKDLVLALTLLVIACPGALVISAPVSIVAGIGNGAKHGVLVKGGEIMEKL
                     GTVKAVAFDKTGTLTVGKPSVTRAEPVGMSEDELLRIAAVGESYSEHPLGKAIISAAA
                     KKLGRISETPEDAEFFSGHGLRFSLDGKTYFIGNRKLFTDNGISVERHESYLQGEEKK
                     GQTAVIVGTAEGVCGIISIADTVRPEAKALIADLESRGIEKIVMLTGDNIRAAQAIAK
                     ETGIREFYSELLPDDKVRILSELRERYGSVAMVGDGVNDAPALASSDLGIAVGGAGKD
                     IAMETADVVLLSAGIRKLSYAVGLSRATVRNMKQNITFALLVAAFLLAGVLAKTVTLS
                     FGMLVHEVSVLLVILNAVRLLHYGESKKPEDVLSGEGGIAVEEL"
BASE COUNT          493 a          703 c          595 g          587 t
ORIGIN      
        1 cagcacgtgg caggcagtgt ttcagaggat ggagaaggtg tacttcacgc tggcagcttc
       61 tgaatcccgg tgaaggcgag attcttcggg cttcgccctc agaatgacag gcaaatactg
      121 tcgtcctgaa cggagcgacg cgcagggaaa tccagaggct catttgcccc gtgccggagc
      181 cggggcagcg gctttcaagg cccttttgca ggcaggttct cagagaagtt tctgcaatcc
      241 ttccctgttc tcaatgatta tccttttatg tcccttgagt gaaatgagcc cttcttcctg
      301 aaaggaagac agtttccggc tcagggtttc ctggctcatc cccagctggg aggcgaagtc
      361 tccttttgtc atggggagaa tgatttccct ctcttcacca gataaatcga gcagaaccct
      421 cgccagcctc tcttccacgg aattcaggct gatttccttt atcaggtttt cggcccgttc
      481 gagccttttg ctcagttcgt ccataacctt gaaagcgatg gagatatatt tcgacatcag
      541 cagcttcagc ttcccgccgt cgataaagca catggtggag ttttcaaggg ctgccgcatc
      601 gtccgtaaac ggaaggtaac tgaacagtga aagctctccg atgaattctc ccgggcctat
      661 gactcgtatg acctgttcct tgccgtcgga actgagcctg gatattttca ccccgccggt
      721 gtggagaata tagagtttcc cgccggtgtc cccctccctg taaatgaact ctccctttag
      781 aaaggtgcgg ggttcggtaa tggaggctat ctccgacatt tcgtcagggc tcagggattg
      841 aaaaagaggc acattttcaa aacagttccg ccagttctcg ccaccactgc agctacagtt
      901 cttcaacggc aattccccct tcaccggata gaacgtcctc cggctttttc gattccccgt
      961 aatgaagcag cctgaccgcg ttgagtatga ccaggagtac ggaaacttca tgtaccagca
     1021 ttccgaatga cagcgtaacg gttttggcca gcactcccgc cagaagaaac gccgccacaa
     1081 gaagagcgaa ggtgatgttc tgcttcatgt tccggacggt agcccggctc aggccgaccg
     1141 cataggacag tttcctgatt cctgccgaca agagtaccac gtccgccgtt tccatcgcta
     1201 tatccttgcc ggctcctccg acggctatcc ccaaatccga ggacgcgagc gccggtgcgt
     1261 cgttgactcc gtcgcccacc atggcgacgc ttccgtaccg ttcacggagt tcggacagaa
     1321 tccgcacttt gtcatcagga agcagttcgc tgtaaaactc gcggatgcct gtttcctttg
     1381 caatcgcctg ggccgctctt atgttgtctc cggtcagcat gacaatcttt tcaattcccc
     1441 gcgattcaag gtcggcaatc agagccttcg cctcaggcct cacggtgtcg gcaatggata
     1501 tgattccgca tacaccctcg gcagtaccca cgataaccgc cgtctgcccc tttttctcct
     1561 cgccctgcag ataactttcg tgtctctcaa cactgattcc gttgtccgtg aacaatttcc
     1621 tgttgccgat gaagtaggtt tttccgtcaa ggctgaatct gagcccatgg ccgctgaaaa
     1681 attccgcatc ctcaggagtt tcgctgatcc gccccagttt ttttgccgcc gctgagatga
     1741 tggcttttcc cagcggatgt tccgagtagg attcaccgac cgcagcaatc cttaaaagtt
     1801 catcttcgct catgcccacc ggctctgccc gtgtcacgga gggtttcccg acggtcaggg
     1861 ttcctgtctt gtcaaaagcg acagccttta cggttccaag tttttccata atttccccgc
     1921 ccttgacaag aaccccgtgt tttgccccgt ttcctatgcc ggccacgatc gagacggggg
     1981 cggagatcac aagagcaccc ggacaggcta tgaccagaag tgtcagggcc agcaccagat
     2041 ctttcgtggc gaggaacagg gcaatggaaa gaaagattat cgccggggtg taatagcggg
     2101 agaacttttc caggaacttc tgtgttatcg ccttcttgtc ctgggcatcc tcgaccatct
     2161 gcagaatcct ggcaaaagtg gtgtcctcgc ccaccctgtc tgccctgaca atcagatatc
     2221 ccgactcgat gacggtcccc gaaaacaccg tttcatccac ggtgcggctt atgggaatgg
     2281 actctccggt aatggccgcc tggttgatgt aggccgagcc ctccaccacg gttccgtcga
     2341 ccgctatttt ttctccgggc ctcaccacca cgaggtcg
//
LOCUS       DAOTHD010000289         2377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_53054_length_2377_cov_3.042205, whole genome shotgun sequence.
ACCESSION   DAOTHD010000289 DAOTHD010000000
VERSION     DAOTHD010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53054_length_2377_cov_3.042205"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="P5219_05805"
     CDS             complement(<1..534)
                     /locus_tag="P5219_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HRX26303.1"
                     /translation="MQLFILVTYILILYGISWYASKLIQKGKGGAVQYLLAGRNLPTV
                     VVAAMLAGLAIGGASTVGVAEHAYKVGLSAGWYNAAWGTAGIIVGLFVADYFRRMEVN
                     TIPEMMERMYGKQARMISVLAQLLIMMVITSLQYVAGGAVLTALMPSVFTFQTGMLVT
                     AVLFVGITFIGGYWGAGL"
     gene            complement(667..1752)
                     /locus_tag="P5219_05810"
     CDS             complement(667..1752)
                     /locus_tag="P5219_05810"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF015727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HRX26304.1"
                     /translation="MKLAMVFYRHHLTRENFLYARQLGCTHLVIHLVDYFHREKDPVT
                     GNNQPVGDADGWGVTRNRDVWSYEELRDIRKSIEDEGLVLEAIENFDPGFWYDVLLDG
                     PKKAEQMEGLKGLIRNIGRAGIPIMGYNFSLAGVASRISGPFARGGAESVGVDGCDSL
                     PLPRGMVWNMFYDPEAPKEVNSDTQITSEELWKRFAWFLEELLPVAEESSVKMAAHPD
                     DPPFATLRGTPRLVWRPELYQRLLDLKKSHSNTLELCVGTLAEMNGCNFYDAIESYAK
                     QNAIGYIHLRNVEGQVPRYRETFIDEGDIDIYRVLKILRRNDFRGAVIPDHSPLVSSP
                     APWHVGMAYALGYIKAMMQRVEKDCRD"
     gene            complement(1838..>2377)
                     /locus_tag="P5219_05815"
     CDS             complement(1838..>2377)
                     /locus_tag="P5219_05815"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRX26305.1"
                     /translation="PQRGTYVSLMDMEQIEEVRFTRYCVESTVLSLFISSPPDEKTLT
                     AMEALLETQEQAIAGRDVDGFVDSDDAFHRELFLACGKARAWSFIDHFSAQYKRLRYL
                     SLQNYLDFGNVLDEHRAILSACRRRDSDDALRWLSRHLNKILGESEHLREKFPAYVKK
                     SGVSRDSAAVGTTIPVQGA"
BASE COUNT          423 a          719 c          734 g          501 t
ORIGIN      
        1 gagccccgct ccccagtagc cgccgatgaa ggtaatcccc acgaagagaa cggcggtcac
       61 cagcattccc gtctggaagg tgaacacgga gggcatgagg gccgtgagga ccgcccctcc
      121 cgccacgtac tggagggaag tgatgaccat catgatgagg agctgggcga ggacgctgat
      181 catccgggct tgctttccgt acatccgttc catcatctcg gggatggtgt tcacttccat
      241 gcggcggaag tagtccgcca cgaagaggcc cacgatgatg cccgccgttc cccaggcggc
      301 gttgtaccac ccggcggaga ggcctacctt gtaggcgtgt tcggccacgc ccaccgtgga
      361 ggctcctccg atggcaaggc ccgcgagcat ggcggccacc accaccgtgg ggagattccg
      421 cccggcgagg aggtactgga cggcgcctcc cttgcccttt tggatgagct tcgaagcgta
      481 ccaggagatg ccgtagagga tcaggatgta cgtcaccagg atgaacagct gcattgatat
      541 ccccccaaaa agaattttcc cagtagtata acatcttctc aggagaaaaa aagagccgga
      601 tctctttcaa ttccgctgtc tgcgggctga aaagaaatcc ggctcttcgt atcattttct
      661 tctgccttag tccctgcagt ccttctccac tctctgcatc atggccttta tgtacccgag
      721 ggcgtaggcc atgcccacgt gccacggggc cggactcgac accaggggcg agtggtcggg
      781 gatgaccgcg ccccggaagt cgttccgccg gaggatcttc agcacccggt aaatgtcgat
      841 gtcaccctcg tcgataaagg tctcccggta ccggggcacc tgtccctcca cgtttcgcag
      901 atggatgtag ccgatggcgt tctgcttcgc gtagctttcg atggcgtcgt agaagttgca
      961 gccgttcatc tccgccaggg tgcccacgca gagttccagg gtgttgctgt ggctcttttt
     1021 caggtccagg agcctctggt agagctcggg ccgccacacg agcctgggcg ttccccggag
     1081 tgtcgcgaag gggggatcgt ccgggtgggc cgccattttg acgctggact cctccgccac
     1141 gggaagcagc tcctcgagga accaggcgaa ccgcttccag agctcctcgg atgtgatctg
     1201 ggtgtcagag ttcacttcct tcggcgcttc ggggtcgtag aacatgttcc agaccatccc
     1261 gcggggaagg ggaaggctgt cgcagccgtc gacgcccacc gattctgcgc ccccccgggc
     1321 gaaggggccg ctgatcctgg aggccactcc ggcgaggctg aagttgtagc ccatgatggg
     1381 gatgcccgcc ctgccgatgt tccggatgag ccccttgagc ccctccatct gctcggcctt
     1441 tttcggcccg tcgaggagga cgtcgtacca gaagccgggg tcgaagtttt ctatggcctc
     1501 gaggaccagt ccctcgtcct cgatggactt ccggatgtcc cgaagctcct cgtagctcca
     1561 cacgtccctg ttccgggtca ccccccagcc gtcggcgtcg cccacgggct ggttgtttcc
     1621 cgtcacggga tccttttcgc ggtggaaata gtccaccagg tggatcacca ggtgggtgca
     1681 gccgagctgc ctggcgtaca ggaagttttc cctggtgaga tgatgcctgt agaacaccat
     1741 tgccagtttc atcggtccga tctccttgtc tccatgtcag atgaaagcaa tcataccaga
     1801 ttcgtccgcc gtccaggatc gccgtttccg aattccgtca cgctccttga actggaatgg
     1861 tggtgccgac tgccgcggag tcccgggaaa ctccgctttt cttcacatag gccgggaatt
     1921 tttcccggag gtgctccgat tcgccgagga tcttgttgag gtgccgtgag agccacctga
     1981 gggcgtcgtc gctgtcccgg cgcctgcacg ccgagaggat ggccctgtgt tcgtcgagca
     2041 cgttgccgaa atcgaggtag ttctggaggc tgagatacct gaggcgcttg tactgggcgc
     2101 tgaagtggtc gatgaagctc caggcgcggg ccttgccgca ggcgaggaac agttccctgt
     2161 ggaaagcgtc gtcggagtcc acgaagccgt caacgtcccg tccggcgatg gcctgctcct
     2221 gggtttccag gagcgcctcc atggcggtca gtgttttttc gtcgggcggg gaggaaataa
     2281 aaagggagag aacggtgctt tcaacgcaat acctggtaaa ccggacttcc tcgatctgtt
     2341 ccatgtccat gagggatacg taggtgcctc tctgggg
//
LOCUS       DAOTHD010000290         2376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_53080_length_2376_cov_2.874623, whole genome shotgun sequence.
ACCESSION   DAOTHD010000290 DAOTHD010000000
VERSION     DAOTHD010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53080_length_2376_cov_2.874623"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="P5219_05820"
     CDS             complement(<1..362)
                     /locus_tag="P5219_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HRX26306.1"
                     /translation="MQSQKDNSVFFISMAVTAATVLWGIISPASFDAAAKGLFGFLIE
                     YFGWGYMLAMNIFVLFPILLCMSRFGKLRLGEPGSKPEFSNISWFAMLFSAGMGVGLV
                     FYGVGEPVFHFMTPPFGAA"
     gene            complement(419..1747)
                     /locus_tag="P5219_05825"
     CDS             complement(419..1747)
                     /locus_tag="P5219_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(686..688),aa:Sec)
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HRX26307.1"
                     /translation="MLKAIHYINQFFGQVGGEDMADYEPEIRTGTVGCTQIFNSLAKD
                     VEVTHTIICGDNFMASRTDEALARILAMLEGVEFDIFIAGPAFNAGRYGAACGEVCKA
                     VKEKYDVPVITSMNEENPGVELFHKDMYIFRGGNRATFMKQDMEKLAAFADRIARGGK
                     LLPAVMEGYFPRGIRHEVFLEDIGREPVMAADRVVDMMIAKLRGEPYQTELPMPALDR
                     VPVAPAVKDLRKVRLALVTSGGIVPRENPDRIQSCSATKWGKYDISGLEKGFTAPEYK
                     TIHAGYDPEQADRNPNVVVPLDAIRAYQREGRIGEVDDYYYTTVGTGTTQGEAARMGR
                     EIAAVLHERNVEAVVLTATUGTCTRCGSTIAKEIERSGIPVVVMCNLTNIAKTVGVNK
                     MVPTVSVPYPLGNPQLRPDDEWALRYHRTGVALDALETDVSEQTIFPVKI"
     gene            complement(1765..>2376)
                     /locus_tag="P5219_05830"
     CDS             complement(1765..>2376)
                     /locus_tag="P5219_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HRX26308.1"
                     /translation="HPNELLDGAVVSGSFMPVSSKMSTYEHCNNPVVKRLMKEHGKTL
                     NFLGIVMSNLNVAMDQKQRSAYMVAQMAKNLGADGAIVAEEGYGNPDVDYIQTIVELE
                     KVGVKTVGISNECTGRDGGSQPLVALDPAANALVSTGNVSQYFELPPMKTVLGELESM
                     ARDGLSGGWEGCVRADGSLVMENNGMFCSDHISGFSVKTCMDY"
BASE COUNT          435 a          709 c          745 g          487 t
ORIGIN      
        1 gccgctccga agggaggggt catgaagtgg aacacaggct cgccgactcc gtagaagacg
       61 agaccgacgc ccatgcctgc cgagaagagc atggcgaacc aggagatatt gctgaactcc
      121 ggcttcgaac cgggctcgcc gaggcggagc tttccgaagc ggctcatgca gaggagaatg
      181 gggaagagaa caaagatgtt catggcgagc atgtaccccc acccaaagta ctcgatgagg
      241 aatccgaaga gccccttggc cgccgcgtcg aaacttgcag gggaaatgat accccagaga
      301 accgtggctg cggtgaccgc cattgagatg aagaacacgg aattatcttt ctgagactgc
      361 atactcgtcc ctccttcact tggttccggg ggagggaatc ctcccccgga gaggtgttct
      421 agatcttcac aggaaagatg gtctgctcgc tgacatccgt ctcgagggcg tcaagcgcca
      481 cacccgtcct gtggtagcgg agggcccatt catcgtccgg gcggagttgg ggattgccca
      541 ggggataggg gacggagacc gtgggaacca tcttgttgac gccgacggtc ttcgcgatgt
      601 tggtgaggtt gcacatgaca accacgggaa tgccgctgcg ctcgatttcc ttcgcgatcg
      661 ttgacccgca acgagtgcag gttcctcagg tcgctgtgag aacaacggct tccacgttcc
      721 gttcatggag caccgcggcg atctcacggc ccatgcgggc agcctctccc tgggtggtgc
      781 cggtgccgac ggtggtgtag tagtaatcgt ccacttcgcc gatcctgccc tcccgctggt
      841 aggcccggat ggcgtcaagg ggaaccacca cgttggggtt cctgtcggcc tgctctggat
      901 cgtagccggc atgaatggtc ttgtactccg gggcagtgaa tcctttttca agtccggaga
      961 tgtcgtactt cccccacttc gtggcggagc aggactggat tctgtcaggg ttttcccggg
     1021 ggacgatgcc gccggaggtt accagggcca ggcgcacttt ccgcaggtcc ttcaccgccg
     1081 gggcgacggg gacacggtca agggccggca tgggcagctc ggtctggtag ggttcgcccc
     1141 tgagcttggc gatcatcatg tccacgaccc ggtcggccgc catgacgggc tccctgccga
     1201 tgtcctccag gaagacctcg tgacggattc ccctggggaa gtagccttcc atgactgccg
     1261 gaaggagctt cccgccccgg gcgatcctgt cggcgaaggc ggcgagtttc tccatgtcct
     1321 gtttcatgaa agtggcccgg ttgccgcccc ggaagatgta catatccttg tggaacagct
     1381 ccactcccgg attttcctcg ttcatggacg tgatcacggg aacgtcgtat ttctccttca
     1441 ctgccttgca gacttcgccg caggctgccc cgtaccggcc tgcgttaaaa gcggggccgg
     1501 cgataaagat gtcgaactcc acgccctcga gcatcgccag gatgcgggcc agggcctcgt
     1561 ccgtgcggga cgccatgaaa ttgtcgccgc agatgatggt gtgggtcacc tccacgtctt
     1621 tggcgagact gttgaaaatc tgggtgcacc cgacggttcc ggtgcggatt tcaggctcgt
     1681 agtcggccat gtcctcgccg ccgacctgcc cgaaaaactg gttgatgtaa tgaatcgcct
     1741 tgagcattat gccttctccc cttcctagta gtccatgcag gtcttcacgg agaagccgct
     1801 gatgtggtcc gagcagaaca tgccgttgtt ctccatgaca agggaaccat cggcgcggac
     1861 acacccttcc cagccgcccg agaggccgtc gcgggccatg gattcaagtt cgccgaggac
     1921 ggtcttcatc gggggcagct cgaaatactg ggatacgttc cccgtggaaa ccagggcgtt
     1981 cgccgccgga tcgagggcca ccaggggctg cgatcctccg tcgcggccgg tgcactcgtt
     2041 ggagatgccg acggtcttga cgccgacctt ctccagctcg acgatggtct gaatatagtc
     2101 cacatccgga ttgccatacc cttcttcagc cacgatcgcg ccgtcggcgc cgaggttctt
     2161 cgccatctgg gccaccatgt aggcggaacg ctgcttctgg tccatggcca cgttgaggtt
     2221 ggacatgacg atcccgagga agttcagcgt cttgccgtgc tccttcatca ggcgcttcac
     2281 cacggggttg ttgcagtgtt cgtaggtgga catcttggag gagacgggca tgaagctgcc
     2341 ggagaccact gccccgtcga gcagctcgtt ggggtg
//
LOCUS       DAOTHD010000291         2375 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_53102_length_2375_cov_2.763793, whole genome shotgun sequence.
ACCESSION   DAOTHD010000291 DAOTHD010000000
VERSION     DAOTHD010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53102_length_2375_cov_2.763793"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1760
                     /locus_tag="P5219_05835"
     CDS             <1..1760
                     /locus_tag="P5219_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRX26309.1"
                     /translation="HNAKPLLLAKEMELRLADAVKPSLTAGDIMTSPVMVIPPDSSVD
                     DAYRIMIRYGHSALPVVKGKAILGLITRKDLDKAHLHGFGKTLIREFMTEGIISISKE
                     ASVHEAHRLFVTHSIGRLPVTEGNRIVGIITRTDLVKALYPLSVPREERSGAPELPWT
                     EDVSWLLEKSLAPEAIGLLRTLGERAEKLGMRAYIVGGIVRDLFLGRDNLDLDIVVEG
                     DAPRFLRSWEKDGLRVSVHERYKTGTVVFPGGKKVDVATARREFYEFPVAQPKVFTDS
                     LKHDLYRRDFTVNAMAVSINLSTWGILVDFFGGRRDLGKKILKVLHNLSFVEDPTRVL
                     RGIRLEQRLGLSLEDNTLRLLRSCVRGGLLVRLSGFRLRSELELSFMERFPYPAAHRM
                     EELGVWEVLFPGLRLGETGRRTFRRLGAFPGRISRDFPDFRGRQWLAFFSALLMESPE
                     NIRLAAVDRLNLPESERGIVIKCLSELGTVEHELGGRSGPSNSRIYAFLRDADPAVCL
                     FWSAATGRWRVRRRILLYLTRLHRTSPELKGRDLIELGYGTTPRIGVILEKLKILKLD
                     GILENRDDEVHYVRYNFPL"
     gene            1785..>2375
                     /locus_tag="P5219_05840"
     CDS             1785..>2375
                     /locus_tag="P5219_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRX26310.1"
                     /translation="MLRMPALADLLLSIPAVLWAITFHEFCHGYMAYRLGDPTAARMG
                     RLTLNPLHHFDPIGALMLLLFRFGWAKPVPVDPRYFKHPRRDMFLVSIAGVSGNFLTA
                     FLFGMLARLFPYPFLAVPALRQFIFLMIVINIGLAVFNLIPIPPLDGSKLLYPILPPA
                     LLEKYFWLERYGFFILMILVALGVVQAVMGPVVMFFL"
BASE COUNT          471 a          703 c          674 g          527 t
ORIGIN      
        1 ttcacaacgc caagcctctt ctcctcgcaa aggaaatgga gcttcgtctc gccgacgcgg
       61 ttaagccttc gctgacggca ggcgacatca tgacctcgcc cgtcatggtg ataccgccgg
      121 attcctcggt ggatgacgcc tacaggatca tgatacggta cggccattcc gccctccccg
      181 tggtgaaggg aaaggccatc ctcggcctca tcacgaggaa agatctggac aaggctcacc
      241 ttcacggttt cggcaagacc ctcatcaggg aattcatgac cgagggaatc atcagcattt
      301 ccaaagaagc ctcggtgcac gaagcccaca ggcttttcgt cacccacagc ataggccggc
      361 tgcccgtcac cgagggaaac cgtattgtcg gcatcataac ccgcaccgac cttgtgaaag
      421 ccctttatcc cctgtctgtt ccgcgggaag agagaagcgg cgccccggag cttccctgga
      481 ccgaggatgt ttcgtggctt cttgagaaaa gcctcgcccc ggaagctatc ggccttctga
      541 gaaccctggg ggaacgggcg gaaaaactgg gaatgagggc ctacatcgtc ggcggcatcg
      601 ttcgggatct ttttctcggg cgggacaatc tggacctgga catcgtggtg gagggagacg
      661 ctccccgatt tctgagaagc tgggaaaagg acggcctccg cgtatcggtt cacgaacggt
      721 acaagacggg aacagtggta ttcccgggag gcaagaaagt cgatgtggcc acagcccggc
      781 gggagtttta cgagtttccc gtcgcccagc cgaaggtgtt caccgattcc ctgaagcatg
      841 acctctaccg gcgggacttt accgtcaacg ccatggccgt ctccataaac ctgtccacgt
      901 ggggcatcct cgtggatttt ttcggcggac ggagggatct cggcaagaaa atcctgaagg
      961 tcctccataa cctgagtttc gtggaagacc ccaccagggt actgagagga atccgcctgg
     1021 agcagcgcct tggcctctcc ctggaggaca acaccctgag gctcctgaga agctgcgtgc
     1081 ggggaggtct tctcgtacgg ctttcgggct tccgcctcag aagcgagctt gagctgtcct
     1141 tcatggagcg ttttccctac ccggcggcac accggatgga agagctggga gtatgggaag
     1201 tgctgttccc ggggctccgt ctcggcgaaa ccggaaggag aaccttcaga cgcctcggtg
     1261 cctttcccgg ccgtatttca agagattttc ccgatttccg gggcagacag tggcttgcct
     1321 tcttttccgc tcttctcatg gaatctccgg agaatatcag gctcgccgcc gttgacaggc
     1381 tgaatcttcc cgagtcggaa cggggcatcg taattaagtg cctttccgaa ctgggaacgg
     1441 tggaacacga actcggaggc cgctccgggc cttcgaattc acggatctat gcctttctca
     1501 gggacgctga tccggcagtc tgtctcttct ggagcgccgc taccgggaga tggagggtgc
     1561 ggagaaggat tcttctgtac ctgaccaggc tccaccgcac atctcccgag ctgaaaggca
     1621 gggacctgat cgagctcgga tacgggacaa cgccgagaat aggggttatc ctcgaaaagc
     1681 tgaaaatcct caagctcgac ggtatccttg aaaatcggga tgatgaggtt cattacgtgc
     1741 ggtataattt tcccctgtga ttttttgaag gagagtgaaa aacaatgttg cgtatgcccg
     1801 cgcttgcgga cctgctgctg agtattcccg ccgttctctg ggccatcacg ttccacgagt
     1861 tctgccacgg ctacatggcg tacaggctgg gagaccccac cgccgcccgg atgggcaggc
     1921 tgaccctcaa tcccctgcac catttcgacc ccatcggagc cctcatgctc cttctcttca
     1981 ggttcggatg ggcaaagccg gtacctgttg acccgaggta tttcaagcat ccccggagag
     2041 acatgttcct cgtctccata gcgggagtga gcgggaactt cctcacggcc ttcctgttcg
     2101 gaatgctcgc aaggctcttt ccatacccgt tcctcgctgt tccggcactg agacagttca
     2161 tcttcctgat gatcgtcatc aatatcggcc tcgccgtctt caacctcatc cccataccgc
     2221 ctctcgacgg ctcgaagctg ctgtacccca ttttgccgcc ggcgctcctg gagaagtatt
     2281 tctggcttga acggtacggg tttttcatcc tcatgatcct ggtggctctc ggtgtggtcc
     2341 aggccgtcat ggggcccgtg gtgatgtttt tcctc
//
LOCUS       DAOTHD010000292         2372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_53186_length_2372_cov_2.639620, whole genome shotgun sequence.
ACCESSION   DAOTHD010000292 DAOTHD010000000
VERSION     DAOTHD010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53186_length_2372_cov_2.639620"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="P5219_05845"
     CDS             <1..249
                     /locus_tag="P5219_05845"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555788.1"
                     /note="catalyzes the formation of 5-phospho-alpha-D-ribose
                     1-phosphate from D-ribose 5-phosphate and ATP; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRX26311.1"
                     /translation="AAALIKDRGAVKVFACATHGVLSGPALERLENSGIDEVVLTDTI
                     PLPESKKSDKIIQLSIAPLFAEAILRVHSDRSVSSLFD"
     gene            311..964
                     /locus_tag="P5219_05850"
     CDS             311..964
                     /locus_tag="P5219_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRX26312.1"
                     /translation="MSEIMKVVLEAREGKGKEACSKLRPAGYVPCVVYGPEYRESVPA
                     KVKVSDISKIAASGHWETQTMLLTLPGGKEEMGLMREVQKDTITGRILHIDFLQLVKG
                     HKISVNVPVELVGRDACAGVKLGGVIDHLLREISMEVLPGQIPDSVAVDVSSMKLGAQ
                     VHVKDIAVPEGAAVLSDPDEVVVAVIIPRGVAEGEETAEEQKEVEVVAKGKAKEEEE"
     gene            971..1537
                     /gene="pth"
                     /locus_tag="P5219_05855"
     CDS             971..1537
                     /gene="pth"
                     /locus_tag="P5219_05855"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRX26313.1"
                     /translation="MKIIAGLGNPGPEYVFSRHNAGWLVVDHLVGRYSCGSPRMQFSS
                     MAWTHFRNGEKLLLLKPLTYMNLSGRALREAADYFSLSWEEDVLVVYDDVALPYGKLR
                     LRKKGSAGGHKGMLSVLGTAGTLEVCRLRIGVGAAPGENMVSWVLGSFSPGERSGLPD
                     LLDRAADAVELWCTEGADKAMNAINATV"
     gene            1585..>2372
                     /locus_tag="P5219_05860"
     CDS             1585..>2372
                     /locus_tag="P5219_05860"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26314.1"
                     /translation="MRNASAPAGNRIWDLDERLWTGGTALHLPLDGAARCWICRNPAS
                     PALVVFPDTKQAALFCSDWETLFPGERVLLLHEIPLTPHGVQDRALPVQRGETLARWK
                     EEGGILAASGGALLGPVYKGSRDIRITTGKEYRRENLLDWLVHAGYERSDIVWAPGQF
                     IFRGYILDVFDPAYAHPLRFEFFDETVESVRSFSPRTQTSVGLLDGADIHSLSTSKEA
                     SLFSFFPENAVSVFFEPAKTEVSADSYALLWKDIVEAEGRGVALP"
BASE COUNT          454 a          721 c          743 g          454 t
ORIGIN      
        1 gccgctgccc tcatcaagga caggggagcg gtgaaagtgt tcgcctgcgc cacccacggc
       61 gtgctctcgg ggcctgcgct ggagcgtctc gaaaattccg ggatcgacga ggtcgttttg
      121 accgacacaa tcccattgcc tgagagcaag aaatcggata aaatcattca attgtcaata
      181 gccccccttt tcgcggaagc cattctgagg gtccacagcg accgttcggt gagcagcctc
      241 ttcgactagg ctgttgttga ttttcctgct tctgcggcgg ggatcgtatt ttaaggagga
      301 tgtgtcggtt atgtcggaaa ttatgaaagt cgttcttgaa gcgcgtgaag gaaaaggcaa
      361 ggaagcctgc agcaagctgc gccccgcggg gtatgttccc tgtgtcgtgt atggtcccga
      421 gtaccgcgag tccgtgccgg ccaaggtgaa ggtatcggat atttccaaga ttgccgcttc
      481 cggccactgg gagactcaga ccatgcttct caccctcccc ggcggcaagg aggagatggg
      541 gctcatgaga gaggtccaga aagacaccat caccggcaga attcttcaca tcgacttcct
      601 gcagctcgtg aagggccaca agatttcggt gaacgttccc gtggaacttg tcggccgcga
      661 cgcctgcgcc ggagtgaagc tcggcggcgt tatcgaccac ctcctccgtg aaatttccat
      721 ggaagtgctt cccggccaga tccctgattc cgtggccgtg gacgtctctt ccatgaagct
      781 cggtgcccag gtccacgtga aggacatcgc cgttcccgag ggagccgccg tcctctccga
      841 cccggatgaa gtcgtggtcg ccgtgatcat cccgagaggc gtcgccgaag gcgaagagac
      901 ggcagaggaa cagaaggaag tggaagtcgt cgccaaggga aaggccaagg aagaggagga
      961 gtaaccccgg ttgaagatca tcgccggatt gggcaatccc gggccggagt atgtcttttc
     1021 aaggcacaac gcgggatggc tcgtggtgga tcaccttgtg ggccggtatt cctgcggctc
     1081 cccgaggatg cagttttcct ccatggcctg gacccatttc cggaacgggg aaaaactgct
     1141 gctcctcaag ccgctgacgt acatgaacct cagcggcagg gccctccggg aggcggcgga
     1201 ctatttctcc ctctcctggg aggaagacgt tctggtggtg tacgacgacg tggcccttcc
     1261 ctacggaaag ctccggctgc ggaagaaagg ctccgccggg ggccacaagg ggatgctgtc
     1321 cgtcctcggg acggcgggaa cactggaggt ctgcaggctg cggatcggcg tgggagcagc
     1381 ccccggagag aacatggtgt cctgggtcct cggatctttt tcgcccggcg agcggtccgg
     1441 actgcccgat cttcttgaca gggcagccga cgcggtggag ctctggtgca ccgaaggcgc
     1501 cgacaaggcg atgaacgcta tcaacgcaac ggtctgacga ggcggcggga tacccgccgc
     1561 cttttttgca ggatggagaa tgctatgaga aacgcttccg ccccggcggg aaacagaatc
     1621 tgggatctcg atgagcgcct ctggacggga ggcacggccc ttcaccttcc cctcgacgga
     1681 gcggcacggt gctggatctg cagaaacccc gcctcacccg ccctggtcgt ctttcccgac
     1741 acgaaacagg cggccctgtt ctgctccgac tgggagaccc ttttcccggg agagcgggta
     1801 cttttgctgc acgagattcc cctgacgccc cacggcgtcc aggaccgtgc ccttcccgtc
     1861 cagcggggtg aaacccttgc acggtggaag gaagaagggg gcatcctggc ggcgagcggg
     1921 ggagcccttc tcggtcccgt ctacaagggc agccgggaca tccgcatcac caccgggaag
     1981 gaataccggc gggagaacct tctcgactgg ctcgtccatg ccggatatga gcggtccgac
     2041 attgtctggg ctccgggaca gttcatcttc cgggggtaca tcctcgatgt gttcgacccg
     2101 gcctatgccc atcccctgcg gttcgagttc ttcgacgaaa ccgtcgaaag cgtccgcagc
     2161 ttctcgcccc gcacccagac cagcgtgggc ctgctcgacg gggccgacat ccactccctc
     2221 agcacgtcga aggaagcctc cctgttctcc tttttccccg aaaacgccgt ctccgtcttc
     2281 ttcgagcccg cgaagacaga agtgtccgcc gactcctacg ccctgctctg gaaggacata
     2341 gtggaagccg agggcagggg cgtcgccctt cc
//
LOCUS       DAOTHD010000293         2369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_53275_length_2369_cov_2.995678, whole genome shotgun sequence.
ACCESSION   DAOTHD010000293 DAOTHD010000000
VERSION     DAOTHD010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53275_length_2369_cov_2.995678"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="P5219_05865"
     CDS             <1..1077
                     /locus_tag="P5219_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRX26315.1"
                     /translation="LAEDGEWLSVPCPKCGRPGRREADTMDTFICSSWYFFRYCSPRN
                     GGEVFSKEEAAYWMPVDQYIGGIEHACLHLIYARFFTKFFADLGLSTAREPFTNLLTQ
                     GMVIKDGAKMSKSLGNVVDPDEIISRYGADTARLFILFAAPPANDLDWSERGVEGAHR
                     FLNRVWRLVEENMQVLGKAGPNGPLSMKDLKEPRLRDLKRKIHTTIRDVTRDIDREKQ
                     FNTAVARLMELLNALASFKAESDEEQQLIREGVEVLLSCLNPFCPHITEELWEMTGHG
                     EPLAKLPWPLAEEDALEADTVTVVVQINGKVREKAEFPAGLGEEALKKMALELPGVGK
                     RIEGLEIVRIITVPDRLVNVVVKG"
     gene            1077..2009
                     /locus_tag="P5219_05870"
     CDS             1077..2009
                     /locus_tag="P5219_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26316.1"
                     /translation="MPRLKALTTSGAAGRQLLEDTLAALAVDGYARSGVSEGGDWSAL
                     ISAGRTGSLFDEKRVTVVEGAELLGPFPDALEPFLEEEGAAEVILLVYESAPTKLFSP
                     ETRRKVGFLRAGTTSLAPWERKGWIVTLAKGMNVRLSDDGAAFLAEMLDDPGELRSEV
                     DKLGRYAAGEVVTGDMVKNLSFDEGKSRMLSFLDAFCTGRAEEIFSCLEHLKKDDSIL
                     PLVTALYNRIRPALYLGLFPDRGGDWVRLVLQIKEYPLKMSREALRHYSARALSDLAS
                     GLISLSWKEKTSSAEGWFGFEALLARCMESGESK"
     gene            complement(2075..2350)
                     /gene="rpsT"
                     /locus_tag="P5219_05875"
     CDS             complement(2075..2350)
                     /gene="rpsT"
                     /locus_tag="P5219_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRX26317.1"
                     /translation="MPNKKSAAKRMKTSEKNRLYNRFWTTRCKNAVKKVLEAVENKDT
                     ALAEKRLAIAQSVIDKAVVKGVMHGNTGSRRKSMVTNKVKTLYTQSA"
BASE COUNT          482 a          670 c          717 g          500 t
ORIGIN      
        1 ctcgccgagg acggggaatg gctttccgta ccctgcccga agtgcggccg tccggggcgc
       61 cgggaagcgg acaccatgga tacctttatc tgctcctcct ggtatttctt ccggtactgt
      121 tcgcccagga acggcggaga agtgttctcg aaagaagagg cagcctactg gatgcccgtg
      181 gaccagtaca tcggcggcat cgaacacgca tgtctccatc tgatctacgc ccggttcttc
      241 accaagtttt ttgccgacct tggactgagc accgcccggg agcccttcac caaccttctg
      301 acccagggca tggtcatcaa ggacggagca aaaatgtcca aatccctggg gaatgtggtc
      361 gatcccgatg aaatcatctc caggtacggt gcggacacag cccggctctt catccttttt
      421 gcggctcctc ccgccaatga cctcgactgg tcggagcggg gagtggaagg agcgcacagg
      481 ttccttaacc gtgtctggag gctcgtggag gagaatatgc aggttctggg gaaggccggc
      541 ccgaacggac cgctatccat gaaggacctc aaggaacccc gccttcggga cctcaagaga
      601 aaaatccata ccaccatcag ggacgtgacc agggatatcg accgggaaaa acagttcaat
      661 acggctgtag cccggctgat ggagctcctg aacgcccttg cctccttcaa ggcggagagt
      721 gacgaggaac agcagctcat ccgcgaggga gtggaggttc ttctttcctg cctgaacccc
      781 ttctgtcccc acattaccga ggagttgtgg gagatgacgg ggcacgggga gcccctggcg
      841 aaacttccct ggcctttggc ggaggaagat gcactcgagg ccgatacggt caccgtggtg
      901 gtgcagatca acggcaaagt ccgggaaaaa gcggagttcc ccgctggact cggtgaagag
      961 gctcttaaga aaatggccct cgaactcccc ggggttggca aacggatcga gggactggag
     1021 atcgtcagga tcatcaccgt ccctgacagg ctcgtcaacg tggtggtgaa ggggtagtgc
     1081 ccaggctgaa ggcgctgacc acgtcgggtg ccgccggccg gcagcttctg gaggataccc
     1141 tcgccgccct cgcggtggac gggtatgccc ggtcaggggt ttccgaagga ggggactggt
     1201 cagcgcttat ttccgccggc aggacgggca gcctgttcga cgaaaaacgg gtgacggtag
     1261 tggaaggggc cgagcttctc ggccccttcc ctgatgccct ggaacccttt ctcgaagagg
     1321 agggagctgc ggaagtcatt ctgttggtct atgaatcggc cccgacgaaa ctcttttccc
     1381 cggagacaag gagaaaagtc ggtttcctgc gggcgggaac cacatctctt gctccatggg
     1441 agcggaaggg gtggatcgtg accctggcaa aggggatgaa tgtccgcctg tccgatgacg
     1501 gggccgcctt cctggcggag atgcttgatg accccggcga actccggtcg gaagtggaca
     1561 agctcggcag gtacgccgcc ggggaagtgg ttaccgggga tatggtaaaa aatctttcct
     1621 tcgacgaggg gaagagcagg atgctttctt ttctggatgc tttctgcacg ggccgtgcgg
     1681 aggaaatatt ctcctgcctt gagcacctca agaaagacga cagcattctc ccccttgtca
     1741 ctgccctgta caacaggatc cgccctgctc tgtaccttgg actttttcct gacagggggg
     1801 gcgactgggt gcgtctcgtc ctccagatca aggaataccc cctgaaaatg tccagggaag
     1861 ccctcaggca ctattctgcc cgggcgctgt cagaccttgc ttccggtctc atctcccttt
     1921 cctggaagga gaaaacctcc tcggcggaag gctggttcgg cttcgaagcc cttttggccc
     1981 ggtgcatgga aagcggagaa agcaaataaa aaaagccgag aggacgtccg tccgctcagc
     2041 ttgaatagtc ccgccgtgcc gtaatggtgt gaaatcaggc cgactgagtg taaagggtct
     2101 tcaccttgtt cgtgaccatg gactttcttc tggaaccggt gtttccatgc ataacgccct
     2161 tgacaaccgc cttgtcgatg acgctctggg cgatggcaag acgcttctca gcgagggccg
     2221 tatccttgtt ttcaacagct tcaaggactt tcttcacggc gttcttgcag cgcgtcgtcc
     2281 agaaacggtt gtagagccta ttcttttcgc tggttttcat cctttttgct gcggacttct
     2341 tgttgggcat gaacctcacc tcctcccgc
//
LOCUS       DAOTHD010000294         2369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_53279_length_2369_cov_2.653846, whole genome shotgun sequence.
ACCESSION   DAOTHD010000294 DAOTHD010000000
VERSION     DAOTHD010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53279_length_2369_cov_2.653846"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="P5219_05880"
     CDS             <1..362
                     /locus_tag="P5219_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRX26318.1"
                     /translation="FENAAERGRQFDEKIPALLEIPIVGEVRGMGMMRGVELVADKKT
                     KAPFPASLKAAAFVTEECMKRGLVIYPGTGQIGGVAGDQFLFAPPLVSTKEEMDEMTG
                     RLEEALKASAEALRSKG"
     gene            404..1354
                     /locus_tag="P5219_05885"
     CDS             404..1354
                     /locus_tag="P5219_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRX26319.1"
                     /translation="MKKSILALALIGVLAFASAGSAMTFITIGSGGVGGTYYPLGGAM
                     AEVLTNAGIDVKATSRATSASRENCRLVASGRAHIGMTMGSALYQAITGTEAFEPDGK
                     LPLQILMSMYPAPQHLVTVKGSGITKLEDIKGKKVSVGAPGGGDQILTNLILKAAGID
                     PEKDFTKQQLTQPEGVMALKDGNVDAVFWNFAAPGSAVMEVAAQREIVMVPLDEELVK
                     KIIADNPFLIPYTIKTGTYDSIKEDIRTIADSNYLVVKNDMDETLAYNLTKTLIENRD
                     KFMNITTQAAHFVPAEASVGITEFAAGAKKYFKEQGIDMK"
     gene            1446..>2369
                     /locus_tag="P5219_05890"
     CDS             1446..>2369
                     /locus_tag="P5219_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HRX26320.1"
                     /translation="MTTENNTTLEHSPPPLEKEVIEGRKRELSGWQYWVVAGIALAAS
                     LFHLYTAAFGLLSAMYQRGVHWMFMGSLIFLIYPVSKSRPKNKIDIWDWVLAALMAGG
                     CLNILLNWDAIAMREGMPIASDIYIGTMMIILVIEAGRRSMGWPLPIVAIIATIYALF
                     GPYFPGVLAHGGFPLEELAPFQYLRTDGIFGVPLGVSASFIFLFVLFGAILSVTGAGQ
                     FFIDLSLALTGRSQGGPGKAAVVASGLMGTVSGSSVANAVTTGAFTIPLMKKSGYTPN
                     FAGAVVAASSTGGQVMPPVMGAAATTAPAKLG"
BASE COUNT          509 a          735 c          692 g          433 t
ORIGIN      
        1 tgttcgaaaa cgcggctgaa cggggcaggc agttcgacga aaaaatcccc gccctgcttg
       61 agatacccat cgtgggcgag gtgcgcggca tgggcatgat gcggggcgtg gagctggtgg
      121 cagacaagaa aacgaaagca ccgttccctg cttccctgaa ggcagctgcc ttcgtcaccg
      181 aggaatgcat gaagcggggc ctcgtgatct accccggcac gggccaaatc ggcggagtcg
      241 ccggagacca gttcctgttc gccccccccc tggtctccac gaaggaagag atggacgaga
      301 tgacgggacg ccttgaagaa gccctgaaag cttcggctga ggcgctcagg agcaaggggt
      361 agacagcccc ttcgatcatc agaacaaaag gaggaattgt ttcatgaaga agagtattct
      421 cgcactggcg cttatcggag tactggcatt tgcttccgcg ggatccgcca tgacgttcat
      481 taccatcggc agcggcggcg tgggcggtac ctactaccct ctcggcgggg ccatggctga
      541 agtcctcacc aacgcaggga tcgacgtaaa ggccacatcc agggccacat cggcctcccg
      601 ggagaactgc cgcctcgtcg catcgggcag ggcccatatc ggcatgacca tgggatcggc
      661 cctgtaccag gccatcaccg gcaccgaggc tttcgagccc gacgggaaac tgcccctgca
      721 gatcctcatg agcatgtacc ccgcgcccca gcacctggtc accgtgaaag gcagcggcat
      781 taccaagctc gaggacatca agggcaaaaa ggtctccgtg ggagcccccg gaggcggcga
      841 ccagatcctc accaacctca tcctgaaggc agcgggaatc gacccggaga aggacttcac
      901 caagcagcag ctcacccagc cggaaggcgt catggcgctg aaagacggca acgtggacgc
      961 cgtgttctgg aacttcgcgg cccccggctc ggcggtcatg gaagtggcgg cccagcggga
     1021 aatcgtcatg gtccctcttg atgaggaact ggtcaagaag atcatcgccg acaatccttt
     1081 cctgatcccc tacaccatca agaccggcac ctacgacagc atcaaggaag acatccggac
     1141 catcgcggac agcaactacc tcgtggtcaa gaacgacatg gacgagaccc ttgcctataa
     1201 cctgacgaag acccttatcg agaaccggga caagttcatg aacatcacca cccaggccgc
     1261 ccatttcgtc ccggcagagg ccagcgtggg catcaccgag ttcgccgcag gcgcgaagaa
     1321 atacttcaag gagcagggaa tcgatatgaa ataggccccc tgagggcaaa gagaatcatt
     1381 ccggccggag tgcccctccg gccggttttt cacaggatca catctttccc catggaggtt
     1441 cattcatgac gacggaaaac aataccacct tggaacacag ccctccgcct cttgaaaaag
     1501 aggtgatcga agggcggaag agagaacttt ctgggtggca gtactgggtg gtggcgggca
     1561 tagccctcgc ggcgtcgctg ttccacctct atacagcggc gttcggcctt ctctcggcca
     1621 tgtaccagag gggcgttcac tggatgttca tgggaagcct catcttcctt atctaccccg
     1681 tgtccaagtc ccggccgaag aacaaaatcg acatatggga ctgggtcctg gcggccctca
     1741 tggcgggagg atgcctgaac atcctgctga actgggacgc catcgccatg agggaaggca
     1801 tgcccatcgc atctgacatc tacatcggca ccatgatgat cattctcgtc atcgaggcgg
     1861 ggagacggtc catgggatgg ccccttccca tcgtggccat catcgccacc atctacgccc
     1921 ttttcggtcc ctattttccg ggagtcctcg cccatggcgg ctttcctctc gaggagctcg
     1981 cccccttcca gtacctgagg accgacggca tcttcggcgt tcccctgggg gtgtcagcct
     2041 ccttcatttt ccttttcgtt cttttcggcg ccattctgag cgtcacgggg gcgggccagt
     2101 tcttcatcga cctttccctc gccctgaccg ggcgcagcca gggcggcccc ggcaaggctg
     2161 cggtcgtggc cagcggcctt atgggaacgg tctccggaag ctcggtggca aacgcggtca
     2221 ctaccggagc ctttacgata cctcttatga aaaaatcggg atatactccc aatttcgcag
     2281 gagcggtcgt cgctgcctca tccaccggag gacaggtcat gccgccggtc atgggggcgg
     2341 cagcgacgac cgctcctgcg aaattggga
//
LOCUS       DAOTHD010000295         2367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_53344_length_2367_cov_3.241782, whole genome shotgun sequence.
ACCESSION   DAOTHD010000295 DAOTHD010000000
VERSION     DAOTHD010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53344_length_2367_cov_3.241782"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..1105)
                     /locus_tag="P5219_05895"
     CDS             complement(44..1105)
                     /locus_tag="P5219_05895"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HRX26321.1"
                     /translation="MNSGNRCGTSGSLDSFEDVRKYAVEGIRLHSATHEEIMGGFTTD
                     VYFVKTRDVLRDAGRLSAPVTAEVFARKNGVFAGLEEVMALLGGSSVEIEALREGDSF
                     SPKEVLVRICGPYGEFGLYETVLLGMLASASSWATAARACVEAAEGKPVLCFGARHVH
                     PAVAPVMERTAVRVGGCSGASCILGARLAGIDPMGTVPHAAVLLVGDTVALAKHYDAV
                     MPSGDQRIVLVDTFKDEAEETLRVAEALGDKLSAVRLDTPGERGGVSPDLVREIRYRL
                     DLAGFRHVRIIASGGLYPDRIRELSAAGADSFGVGSYISHANPIDMTMDIKEIDGRPV
                     AKRGRLPGRIENPRLIRVK"
     gene            complement(1112..1336)
                     /gene="secG"
                     /locus_tag="P5219_05900"
     CDS             complement(1112..1336)
                     /gene="secG"
                     /locus_tag="P5219_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRX26322.1"
                     /translation="MKTFLGIIHILLCIALSGVVLLQQRKQGGFSGIFGGGTQADMGG
                     SQWQRFTGLTKITVVLTGLFMVTSIILVLY"
     gene            complement(1380..1982)
                     /gene="rdgB"
                     /locus_tag="P5219_05905"
     CDS             complement(1380..1982)
                     /gene="rdgB"
                     /locus_tag="P5219_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HRX26323.1"
                     /translation="MKPLFPEGLLFASGNRGKFVEVADMFSPIGVDILFGPDHASLEV
                     EETGTTYGANARLKARAWALHTGLPSLADDSGVEVRALGWKPGIHSARMAENDRARIQ
                     WLLDALGDEEDRYARYVAAFALYFPTDGICLLTEGECQGRIVRSPRGTNGFGYDPVFC
                     PWGYEETFGDIPDREKRKISHRAVAGYRLLDILSCTSMIE"
     gene            complement(1963..>2367)
                     /gene="rph"
                     /locus_tag="P5219_05910"
     CDS             complement(1963..>2367)
                     /gene="rph"
                     /locus_tag="P5219_05910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HRX26324.1"
                     /translation="ILDCDVLQADGGTRTAAVSGGFVALFDALRHLKKQGVFPVLPLR
                     FFLSAVSVGLVAGSPLLDLCYREDSAAEVDMNVVMNHRGEYVEIQGTGEGSVFTGSDL
                     SGLLLLAAVGTLEIIEFQKEALGIEDGETAFS"
BASE COUNT          525 a          715 c          654 g          473 t
ORIGIN      
        1 tccgccgacg cggcgaaatg atgcatgttc cgactccggc cttttatttt acacgaataa
       61 gccggggatt ttcaatcctt cccggcaatc ttcctcgttt tgccacggga cgcccgtcga
      121 tctccttgat gtccatggtc atatcaatgg ggttcgcgtg ggaaatgtag cttccgacac
      181 cgaaggagtc ggctccggcg gccgagagtt cccgtatccg gtcgggatag agccccccgg
      241 aggcgataat gcgaacgtgc ctgaatccgg caaggtcgag acggtaccgt atttcccgga
      301 caagatccgg cgaaacgccg ccccgttcgc ccggagtatc gagcctgacg gcggagagct
      361 tgtctccgag agcttcggca acccgaaggg tttcctccgc ttcatccttg aaggtgtcaa
      421 cgagaacgat tctctggtcg cctgaaggca tgaccgcatc ataatgtttt gccagggcaa
      481 ccgtatcccc gacaagaagt actgcggcgt gaggcaccgt acccatggga tctatccccg
      541 ccagtcgggc gcccaggata cagcttgcac cgctgcagcc tccgacacga acggcagtcc
      601 gttccattac cggcgccacg gccgggtgga catgcctggc accaaagcag agcacgggct
      661 ttccttcagc ggcctccaca catgccctcg cggcagtggc ccatgaactc gcactggcaa
      721 gcattccaag aagtaccgtc tcgtagaggc cgaattcacc gtagggaccg cagatccgga
      781 caaggacttc tttgggggag aaggagtcgc cttccctgag ggcttcaatc tccacgctgc
      841 tgccgccaag cagggccatt acttcttcaa ggcctgcgaa tactccgttt ttccgtgcaa
      901 aaacttcagc ggtaaccggg gctgaaagac gccccgcatc ccggaggaca tcccgggtct
      961 ttacaaagta tacatcagtt gtaaagccac ccatgatttc ttcgtgggtg gcggagtgaa
     1021 gtcgtattcc ctcgacagca tacttccgga catcctcgaa ggagtcaaga gacccggaag
     1081 tgccgcagcg gtttccggag ttcatgtcag cctagtagag aacaagaatg atggatgtga
     1141 ccatgaagag accggtcaga accacggtga tcttcgtgag gccggtgaaa cgctgccact
     1201 ggcttccccc catatcggcc tgggtgcctc cgccgaatat tccggagaaa ccgccctgct
     1261 ttctctgctg caggagcacc acgccggaaa gggcgatgca gagcaggatg tggataatac
     1321 caagaaaggt cttcacgttg cgcacctcct aaaaatagga accaattcca gacaagtggt
     1381 tattctatca tagatgtaca ggaaaggata tccagcaatc gatatccggc cacggcccta
     1441 tgggaaattt ttcttttttc cctgtcgggt atatccccga aggtctcctc atacccccaa
     1501 gggcaaaaca cagggtcata tccgaaacca ttcgttcccc ggggactccg gacgattctt
     1561 ccctgacact ccccctcggt gaggaggcag atcccgtctg tcggaaaata gagggcgaag
     1621 gccgccacat acctggcata ccggtcttct tcgtcgccaa gggcatcgag cagccattgt
     1681 atccgcgccc ggtcgttttc cgccatgcgg gctgaatgaa tgccgggttt ccagccaagg
     1741 gcccgcacct ccactccgct gtcgtcggca agagacggga gaccggtgtg aagggcccag
     1801 gcccgtgcct tgagccgagc gttcgcacca taggtggtgc ctgtctcctc cacttcgagg
     1861 gaggcatggt ccggaccgaa gaggatgtcc actccaatgg gtgagaacat gtccgccacc
     1921 tcgacgaact ttccccggtt gccgctggcg aaaagaagtc cctcaggaaa aagcggtttc
     1981 accgtcttca atcccgagtg cctccttctg gaattcaatg atttcaaggg ttcccacggc
     2041 ggcaaggagg agcaggccag acaagtcaga cccggtgaaa acggaacctt ctcctgtccc
     2101 ctgtatttcc acgtactccc cccgatggtt catcactacg ttcatatcca cctcggcggc
     2161 actgtcctca cggtagcaga ggtccagaag gggcgagcct gcaaccaggc ccacactgac
     2221 tgcggaaagg aaaaacctga ggggcaggac agggaagact ccctgttttt taaggtgccg
     2281 gagtgcatca aagagagcga caaatcctcc cgagacggcg gcggttctcg ttccgccatc
     2341 cgcctggagg acgtcgcagt cgagaat
//
LOCUS       DAOTHD010000296         2362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_53482_length_2362_cov_2.878197, whole genome shotgun sequence.
ACCESSION   DAOTHD010000296 DAOTHD010000000
VERSION     DAOTHD010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53482_length_2362_cov_2.878197"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="P5219_05915"
     CDS             <1..709
                     /locus_tag="P5219_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26325.1"
                     /translation="NETPPSLWGKHLTTPSGSGLQGRTVSGSTAAEHRLSVEWVIDLD
                     ANGVASGTADVFVRNGWVAFFFPGGTPTEDAVGRLAAELFPGMRFSGGGASFAPIKYG
                     WKISLPAEPRQSIVNGGAMLVPFPGATPSWLGELGRSSGEYRMRFPFVVEQSFVLKLP
                     PKSDVVMMPSVVNRALDRVKYEESVYHNKRRNTLTAGTKIVLSTDAISDSVGRSLAES
                     VQRWMAYASRTLPLRVK"
     gene            739..>2362
                     /gene="argS"
                     /locus_tag="P5219_05920"
     CDS             739..>2362
                     /gene="argS"
                     /locus_tag="P5219_05920"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRX26326.1"
                     /translation="MGDVTATLRQIVEGALRDVAAEKGLVFSGLPEIAFEKPKRDNQG
                     DLSTNCAMQMSRHFGEKPMALAEKIVARLSSDPYLEKVETAGPGFINFFLSSRWMGEV
                     LADILNRGKDYGSSHLGGGRRVQVEFVSANPTGPLHVGHGRGAAVGDIIANILNFSGW
                     DVQREYYINDAGLQMDILGRSTQARYFEIAGCPEKAPFPSDGYKGGYIYDIAGSVRER
                     EGDRFLSLPPEESLPFFKTFAADTVLATIRKDLADFGVTFDVWFSEKSLYERNLVPSA
                     METLKNRGFAFEQDGALWFKSTDFTDEKDRVLIRSNGVPTYFASDITYHKEKFDRGFD
                     RVIDIWGADHHGYVPRMKAGVESLGRNPEDLEVLLIQFVNLLRDGRQVAMSTRSGEFV
                     TLRDVMDEVGVDATRFFFVMRRSDSHLDFDLELAKQTSNDNPVYYVQYAHARICSVLR
                     EAEARGIAVPAGADVPEGIFASDEEKKLLSRLADFPKEAEKASKDLAPHILVNYVSDL
                     AGDFHSFYNAHRVLGEEEPVVKARLLLVKAAGT"
BASE COUNT          452 a          721 c          736 g          453 t
ORIGIN      
        1 caatgaaacg ccgccctccc tctggggaaa gcatctcacc acgccttccg gcagcgggct
       61 tcagggccgg accgtttcgg gaagcaccgc agcggaacac cgcctctccg tggagtgggt
      121 tatcgacctt gacgcgaacg gagttgcctc aggcacggcg gacgtctttg tccgcaacgg
      181 ctgggtcgcc tttttcttcc ccggcgggac gcccacggaa gacgccgtcg gccgcctggc
      241 ggccgaattg ttccccggaa tgcgcttttc cggcggcggc gcctctttcg cccccatcaa
      301 gtacggctgg aagatctccc ttcccgccga acccaggcag tccatagtga acggtggggc
      361 catgctcgtt cccttccccg gcgcgacgcc gtcgtggctc ggggagctgg ggagatcttc
      421 cggcgaatac cggatgcgct tccccttcgt ggtggagcag agttttgtcc tgaaactgcc
      481 cccgaagtcc gatgtggtca tgatgccctc ggtggtgaac agggcgctcg acagggtgaa
      541 atacgaggaa tccgtgtacc acaacaagcg gcggaacacc ctgaccgccg ggacgaaaat
      601 cgttctcagc acggatgcga taagcgacag cgtgggaaga agcctggccg agtcggttca
      661 gcgatggatg gcctatgcct ccaggactct tcccctgcgg gttaaatagt ctcagagatt
      721 tcgtggaggt ggcggtgtat gggtgatgtt acggcaaccc ttcggcagat cgtcgaggga
      781 gcccttcggg acgtcgcggc ggaaaagggt cttgttttct ccgggctgcc cgagatagcg
      841 tttgagaagc cgaagcggga taaccagggg gacctttcca ccaactgcgc catgcagatg
      901 agcaggcatt tcggggaaaa gcccatggcg ctggcggaaa aaatagtggc acggctctcc
      961 tccgatccgt atcttgaaaa ggtcgagacg gcagggccgg gattcatcaa cttcttcctt
     1021 tcgtccaggt ggatggggga ggtccttgcc gatatcctca accgggggaa ggattacggt
     1081 tcgtcacatc tcggcggagg cagacgggtc caggtggaat tcgtgagcgc caaccccacg
     1141 ggtccactgc atgtggggca cgggcggggc gcggccgtgg gggacatcat cgccaatatc
     1201 ctgaacttct ccggctggga tgttcagcgg gagtactaca tcaacgacgc ggggctccag
     1261 atggatattc tcggacgatc cacccaggcg cgctacttcg agattgccgg atgcccggag
     1321 aaagccccct tcccgtcgga cgggtacaag ggcggctaca tctacgacat tgccgggtcg
     1381 gtcagggagc gtgaaggcga ccgttttctc tccctccctc ccgaagagag ccttcccttt
     1441 ttcaaaacct ttgccgccga caccgtcctc gccaccatac ggaaggacct tgccgacttc
     1501 ggtgtgacct tcgacgtgtg gttctccgag aagagcctgt atgagcgtaa cctggttccc
     1561 tcggccatgg aaaccctgaa aaaccgcgga ttcgccttcg agcaggacgg cgccctctgg
     1621 ttcaagtcca cggacttcac cgacgagaag gacagggtgc tcatccgcag caacggcgtc
     1681 ccgacctatt tcgcctccga catcacctac cacaaggaaa aattcgaccg gggcttcgac
     1741 agggtcatcg atatctgggg ggcggaccac cacggctatg ttcccaggat gaaggcgggg
     1801 gtggaatccc tcgggagaaa cccggaagat ctggaggtcc tccttatcca gttcgtgaat
     1861 ctcctccggg atggccggca ggtggccatg tccacccgct ccggggagtt cgtcaccctc
     1921 cgggacgtga tggacgaggt cggcgtggac gccaccaggt tcttcttcgt catgcgccgg
     1981 agcgactccc acctggattt cgacctcgaa ctggcgaagc agacgtccaa cgataacccg
     2041 gtatattacg tccagtacgc ccacgccagg atctgcagcg ttcttcgcga ggctgaagcc
     2101 cgggggatcg ccgttcccgc gggagcggat gtgccggagg gaatttttgc ctccgatgag
     2161 gagaagaaac ttctttcccg gcttgccgat ttccccaagg aggctgaaaa ggcctcgaag
     2221 gacctggctc cccacatcct ggtgaactac gtctccgacc ttgcgggcga cttccactcc
     2281 ttctacaatg cccacagggt gctcggcgag gaggagccgg tcgtgaaggc ccgcctgctc
     2341 ctggtcaaag cggccggcac gg
//
LOCUS       DAOTHD010000297         2362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_53488_length_2362_cov_2.313827, whole genome shotgun sequence.
ACCESSION   DAOTHD010000297 DAOTHD010000000
VERSION     DAOTHD010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53488_length_2362_cov_2.313827"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>313)
                     /locus_tag="P5219_05925"
     CDS             complement(<1..>313)
                     /locus_tag="P5219_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRX26327.1"
                     /translation="DKVSTSMTINAPASVLLAMYICVGEKQGVPMDVLSGTIQNDILK
                     EYIARGTYIFPPKPSMRLITDIFEFCSENVPKWNTISISGYHIREAGSTAAQEVAFTL
                     AD"
     assembly_gap    314..323
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<324..786)
                     /locus_tag="P5219_05930"
     CDS             complement(<324..786)
                     /locus_tag="P5219_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRX26328.1"
                     /translation="MNTEYKEARAKFEAGVAKSLQKHKERKESFSTGGGLPLGRLYGP
                     DDVDSVDYMRDIGFPGEYPFTRGVQPTMYRGRFWTMRQYAGFASAEESNERYRYLLSQ
                     GTTGLSVAFDLPTQIGYDSDDPMAQGECGKVGVAIDSLQDMEILFDQIPLDK"
     gene            complement(833..>2362)
                     /locus_tag="P5219_05935"
     CDS             complement(833..>2362)
                     /locus_tag="P5219_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26329.1"
                     /translation="AVPRVLARFEEEGRFLGDVLSGRPVFSRAASALRDLKASLGLPV
                     LQNLGIKGLGKAESSPSGRLAVADVLGDRLWVRNRGERAFSGIEMEAPVTALFQGETG
                     GIVLLENGTVLSLGESGKELTELGKLPFSPSDGTMASESAFIAVSASQRRSAIFSLPD
                     LALVSELAIPETEHPFEPVAAAAFGEWLAVADRNNGAVSLVSLREKRTVFSFEADSPR
                     DLAWSALGDLFVVHENGAVTKTTISFDSMEAVRSETVIREAPGAWSLFSIEDRVYCLD
                     VAGFSLWEVYLFPEDSSPAFLSLDTPSVRREEDRESFLLGASVSGPYRTYMSKNRPVV
                     TSVWNERMLTADFRFLARKSPEGAVFFLPPGRRIGGQSHEAATGKEVFLILSEWWTEN
                     RKKLNDIVVAASIPFSQGELRQLAGFCLQNGIRLFAFCDTFSPLPLLRASGLTGGGAL
                     YSLGGDLPGYSSSHRGEVRIPLPSDETSSGFPSRSVLSVYMDVGSASSRDWMPLWPDL
                     L"
BASE COUNT          523 a          659 c          669 g          501 t
ORIGIN      
        1 gtcggcgagg gtgaaggcca cttcctgggc ggcggtgctt ccggcttccc ggatgtggta
       61 cccggaaatg gagatggtgt tccatttcgg cacgttttcg ctgcagaact cgaaaatatc
      121 ggtaatgagc cgcatcgaag gcttcggggg gaaaatgtag gttccccggg cgatgtattc
      181 cttgaggatg tcgttctgga tggttcccga aaggacatcc atgggcacgc cctgtttctc
      241 ccccacgcag atgtacatgg cgaggagcac gctcgcggga gcgttgatcg tcatggaggt
      301 ggagaccttg tccnnnnnnn nnnccttgtc cagggggatc tggtcgaaaa ggatttccat
      361 gtcctgaaga ctgtcaatgg ccactcccac ttttccgcat tcaccctggg ccatggggtc
      421 gtcggaatcg tagcctatct gggtgggaag gtcgaaggcc acggaaaggc cggtcgttcc
      481 ctgggagagg agatagcggt atctctcgtt ggactcttcg gcggaggcga atcccgcata
      541 ctggcgcatg gtccagaacc tgccccggta catggtgggc tggacgcctc tcgtaaaggg
      601 gtattcgccg gggaacccaa tatccctcat gtaatccacg gagtcgacgt cgtcagggcc
      661 gtagagcctc ccaaggggga gtcctcctcc cgtcgaaaag ctttcctttc tctctttgtg
      721 cttttgaagc gattttgcaa cgcctgcctc gaacttcgct ctggcttctt tgtactccgt
      781 attcatgcgt gcatcccctc ctcgaaaatt tgttcagtca atagctgata atttacagaa
      841 gatcgggcca taggggcatc cagtccctgg aagatgccga acctacgtcc atgtagacgg
      901 aaagaacgga ccggctggga aaaccggacg acgtttcatc ggaaggcagg ggaatcctga
      961 cttctccgcg gtgagaggaa gaatatccgg gcaggtctcc tcccagggaa tagagggccc
     1021 ccccgcctgt cagtcccgag gcccgcagga ggggcaacgg agaaaaggtg tcgcagaagg
     1081 cgaaaaggcg aattccattt tgaaggcaaa atcccgcaag ctgccgaagc tctccctggg
     1141 aaaatggaat ggaggcggcc actacgatat cgttgagttt ttttctgttt tccgtccacc
     1201 attcactgag aatgagaaac acttcctttc ccgttgccgc ttcgtgggac tgccccccga
     1261 tcctccttcc gggaggaaga aaaaacactg ccccttcggg actcttccgt gcaaggaacc
     1321 taaaatccgc agtaagcatg cgctcattcc agaccgaggt gacaaccggc ctgttcttgg
     1381 acatgtacgt acggtacggt cctgatactg atgcccctaa caggaagctt tccctgtcct
     1441 cctccctcct gaccgaggga gtatccaggg agagaaaagc cggagaggag tcctcgggga
     1501 acaaatatac ctcccagagc gaaaaaccgg caacatcaag gcaatagacc ctgtcttcga
     1561 tcgaaaaaag gctccatgcc ccgggggctt cccggatcac cgtttccgac cggaccgctt
     1621 ccatggaatc aaaagaaatg gtcgttttgg tcacagctcc gttttcatgg acgacaaaaa
     1681 gatcaccgag agccgaccat gcaaggtccc ggggtgaatc agcctcaaag gagaataccg
     1741 tccgtttttc ccgaagtgac acgagggaaa ccgcgccgtt gttccggtcg gcgacggcga
     1801 gccattcccc gaaagcagct gccgcgacgg gctcgaatgg gtgttctgtt tccggaattg
     1861 ccagttcgga aacaagggcc aaatccggca gagaaaaaat ggctgatctt ctttgagatg
     1921 cgctcaccgc tataaaagcg ctttctgatg ccatggtgcc gtctgagggg gaaaagggca
     1981 gctttccgag ctccgtcagt tcttttccgc tttcgccaag agacagtacg gttccgtttt
     2041 cgagaaggac tatcccgccg gtctctcctt gaaacagggc tgttaccggg gcttccattt
     2101 cgatccctga aaaagcccgt tctccccggt ttcgtaccca cagccggtcg ccgagaacat
     2161 ccgccaccgc caatcttccg gaaggggatg attcggcttt gccgaggccc tttatgccca
     2221 ggttctgaag cactggaagc cccagggaag ctttgaggtc ccgcaaggcg gaagcggcac
     2281 gggaaaatac aggccttccg gaaaggacat ccccgagaaa cctgccttct tcttcgaatc
     2341 gggcgagtac acggggaaca gc
//
LOCUS       DAOTHD010000298         2357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_53616_length_2357_cov_3.558645, whole genome shotgun sequence.
ACCESSION   DAOTHD010000298 DAOTHD010000000
VERSION     DAOTHD010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53616_length_2357_cov_3.558645"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..1227)
                     /gene="vorB"
                     /locus_tag="P5219_05940"
     CDS             complement(151..1227)
                     /gene="vorB"
                     /locus_tag="P5219_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HRX26330.1"
                     /translation="MERQFLKGNEAVAEAAILAGCRFFAGYPITPQNEIPEYMSRRMP
                     AVGGVFLQGESETASVCMVYGAAAMGTLAMTSSSGPGISLKAEGISTLAGACLPAVIV
                     NVMRGGPGLGAIQAAQSDYLHATKALGHGGFRVIVLAPSTVQEAIDLTYKAFGLAVQY
                     RNPVMVLMDGWIGSMMEPVTLPEAKEPVTFSYTGWGAKYFNPSGVGVNVVTSCLLEPE
                     YQELLNIRAGEMYEEWKEKEVLAEEYLLEDAEYVIVAYGTAARAARTAIGELRGKGIR
                     AGMIRPISLYPFPVEHLKRLDRSRVKFLLDTEMTLPVQMMDDIRLAVADAIPVHSCGR
                     SGGVILTNEEIVEAVELAIAKEAR"
     gene            complement(1275..1490)
                     /locus_tag="P5219_05945"
     CDS             complement(1275..1490)
                     /locus_tag="P5219_05945"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRX26331.1"
                     /translation="MKQHPAAIFLDPEKCRGCGLCMEICKRGVMEFREEANACGCRPV
                     IVVHPEKCVLCGLCVAVCPLHVISLKK"
     gene            complement(1487..2116)
                     /locus_tag="P5219_05950"
     CDS             complement(1487..2116)
                     /locus_tag="P5219_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26332.1"
                     /translation="MFLGPSGSGKSEIVLNLAIRAAGDGGPPVHFFDMDQTKPLFRSR
                     EARDAVERSGAVFHSGFEFLDSPVLPDGVPGLLRDEGVRCFLDMGGSVNGARSLGQCL
                     PDAGGLTQALYVVNLYRPFSDERSFLEDLSAITAAARLSAPCIVSNPNLGGETTIEDV
                     KKGHGRLLEILERNGLSLAFLAVRASLLEAASAWADCPVEGLALRLSAG"
     gene            complement(2167..>2357)
                     /locus_tag="P5219_05955"
     CDS             complement(2167..>2357)
                     /locus_tag="P5219_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRX26333.1"
                     /translation="SVPSLAVYSKIPELPAETEQKLRETFPDLTYVEWTDTGHFLMME
                     QPDRFNGLLVDFLERLDT"
BASE COUNT          422 a          792 c          723 g          420 t
ORIGIN      
        1 acggccagta cggccacggc gttcgtccgc tggtccatct cgtccagcag ctcggcgatg
       61 agtcgggtgg ccacgccgtg aaggcagccg gggcaatagg ccagtccccg gtcggtgagg
      121 gatttcggcc gctcgtatat cttctccatg tcagcgcgcc tcctttgcga tggccagttc
      181 cacggcttcg acgatctctt cgttggtgag gatgacccct ccggagcggc cgcaggaatg
      241 gaccgggatg gcgtccgcaa cggcaagccg gatgtcgtcc atcatctgga cggggagggt
      301 catctccgtg tccagcagga acttcaccct gctcctgtcg agcctcttca ggtgctccac
      361 cgggaaggga tacagggaaa ttgggcggat catgcccgcc cggatgcctt tcccccggag
      421 ttcccctatc gccgtccggg cggcccgggc agcggtgccg taggccacga tcacatactc
      481 tgcgtcctcg agaagatatt cctcggcgag gacttccttt tccttccatt cttcgtacat
      541 ttcaccggcc cggatattca gaagctcctg gtactccggc tcgagcaggc aggaggtaac
      601 cacgttcacg ccgacacccg agggattgaa gtacttcgcc ccccagccgg tgtaggagaa
      661 ggtcaccggc tccttcgcct cgggaagggt cacgggctcc atcatggagc cgatccagcc
      721 gtccatgagc accatcaccg ggttcctgta ctgcaccgcc agtccgaagg ctttgtaggt
      781 gaggtcgatg gcctcctgga cggtggaagg agccaggacg atcacccgga agcccccgtg
      841 acccagcgcc ttcgtggcat ggaggtaatc ggactgggcc gcctggatgg ccccgagccc
      901 tgggccgccc cgcatgacgt tcacgatcac cgccggcagg caggccccgg cgagggtgga
      961 aatcccctcg gccttcaggc tgatccccgg ccccgaggag gacgtcatgg caagggttcc
     1021 catggccgcc gccccgtaga ccatgcatac agaagcagtc tcgctctctc cctgcaggaa
     1081 tacgccgccg acggctggca tcctccggga catgtactcc ggtatctcgt tctggggcgt
     1141 tatgggatac ccggcgaaaa accggcaccc cgccaggatt gccgcttccg ccactgcctc
     1201 gtttcctttc aggaactgtc tttccatgat cccttccttt cccgcgggaa gcacggacgg
     1261 atcccgcgat ttcgtcattt tttcagcgat atgacatgca gggggcacac ggcgacgcag
     1321 agcccgcaca ggacgcactt ctcgggatga accacgatta cgggcctgca cccgcaggcg
     1381 ttcgcctcct cccggaactc catcacgcct ctcttgcaga tctccatgca caggccgcat
     1441 cccctgcact tttccggatc caggaagatg gccgccgggt gctgtttcat cctgccgaca
     1501 gccggagggc aaggccctcc acgggacagt ccgcccacgc cgacgccgcc tccaggaggg
     1561 aggcccggac agcgagaaag gccagggaaa ggccgttcct ctccaggatt tccagcaggc
     1621 ggccgtgccc cttcttcaca tcctcgattg tcgtctcccc gccgaggttg ggattgctca
     1681 cgatgcaggg ggccgaaaga cgggcggcgg cggtgatggc cgaaagatcc tcaagaaagc
     1741 tcctctcgtc ggagaagggg cgatacaggt tgaccacgta gagcgcctgg gtaagcccgc
     1801 ccgcgtcggg gaggcactgc cccagggatc gggcaccgtt cacgctgccc cccatgtcga
     1861 gaaagcagcg gactccctcg tcccgcagaa gaccagggac accgtcgggc agcacgggcg
     1921 agtcgagaaa ctcgaagccc gaatggaaga ccgccccgct cctttccacc gcgtcccggg
     1981 cctcccggga gcggaagagg ggcttggtct ggtccatgtc gaagaaatgg accggcggtc
     2041 ccccgtcgcc tgcggcccgg atggccaggt tcaggacgat ctccgacttg ccgctgccgg
     2101 aggggccgag aaacacccaa ttctttgcct gtggacccgg caaactgcac accccgacgg
     2161 ggtccgtcac gtgtccagcc tttcgaggaa atccaccagc agaccgttga accggtccgg
     2221 ctgctccatc atgaggaagt gccccgtgtc ggtccattcc acgtaagtca ggtcagggaa
     2281 ggtctcccgg agtttttgct ccgtctccgc cggaagctcg ggaattttgg agtacaccgc
     2341 cagggaaggc acgctga
//
LOCUS       DAOTHD010000299         2357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_53621_length_2357_cov_3.130321, whole genome shotgun sequence.
ACCESSION   DAOTHD010000299 DAOTHD010000000
VERSION     DAOTHD010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53621_length_2357_cov_3.130321"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            281..2059
                     /locus_tag="P5219_05960"
     CDS             281..2059
                     /locus_tag="P5219_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HRX26334.1"
                     /translation="MNKLTLRTRLWILALVPVLGIIGTSVFSIYSANSVYSDLLARIH
                     QEAFVAQSLVINGDRDLYQALVAKQTLLTRGPGPEFEKSTGDFRENVAQVRERLEEAE
                     KLLAGNRKGWESFRQKDGRESVFQKFAALKAEFPNWEAASEKQIEDVRTGKTAPGSAA
                     EIEDALFQKVRGNINDIGEILDIGAENSALENRSRMDRANMTVFAVVLAVALAALVLA
                     FTTIRAIRRAVDRILSASEAGREGNLTVRTTLDGTDELAAVGGALDGMLDSLRNVVKD
                     IQQKAAALSSLSETTAASCEEVTSTTNEVAESNTRLAEEVTRGRTGAGEASKMVQEMN
                     TIILSAKELASSADQNSRQMASAAARGKETVAQSIGHMENIRNAVGETEKLITELNNY
                     SARIGVVGTTITSLADQTNLLALNAAIEAARAGEAGRGFAVVAEEVRKLAEQSQQGAR
                     EVAELVGKILEGTESAVKSMETSRKGVEEGVSIAHVAGEALEKIMEATKSSVEDIRRI
                     IAATEKEAEQSGHVIALIDDTTSVMENADEQVQNVAASMEETAAAMESVATGATEVSA
                     TAEDLRKITERFVVDGGTPPRGLALR"
     gene            complement(2120..>2357)
                     /locus_tag="P5219_05965"
     CDS             complement(2120..>2357)
                     /locus_tag="P5219_05965"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRX26335.1"
                     /translation="AEQTRQTLKNVQAILEAAGASMDHVVKVTAHLQEVTKDFRAFNE
                     VYREFFKEPFPVRTTVGSDLLDILVEIDVIAELD"
BASE COUNT          525 a          715 c          717 g          400 t
ORIGIN      
        1 tgccggagat caccaccgag gccccttcct tcaggaactc tcccgccacc gccttgccca
       61 atccggaccc cgaggccagc acgaggaccg tcttgtctgt aagtccaaaa tccatgatca
      121 tccctccctt ggaatgctgt tgtagtatag atattatacc ccatccggtt tttctaaatg
      181 tgcatcgtgc attgcatccc ctgcggactg ttccctaaat catgacaata cggtaaaatg
      241 agccaccaat acaaaatttc tgaatacctg gaggtgccct atgaacaaac tgaccctgcg
      301 tacccgtctc tggattcttg cgctggtccc cgtcctcggc attatcggga cgtcggtttt
      361 ctccatctac tcggcgaatt ccgtctattc cgacctcctg gcccggatcc accaggaagc
      421 cttcgtggcc cagagcctgg tcatcaacgg cgaccgggac ctctaccagg cgctggttgc
      481 caagcagacc ctgctcacca ggggtcccgg cccggagttc gagaaatcca ccggcgattt
      541 cagggagaat gtcgcccagg tacgggagcg gctggaagaa gcggagaagc tcctggccgg
      601 aaaccggaag gggtgggaat ccttccggca gaaggacggc agggaatccg tattccagaa
      661 gtttgcggct ctgaaggcgg aattccccaa ttgggaggcc gcatcggaaa agcagatcga
      721 ggacgtgagg accggaaaga ccgcaccggg atcggcggcg gaaatagaag acgccctttt
      781 ccagaaagtg aggggcaaca tcaacgacat cggcgagatt cttgacatcg gagcagaaaa
      841 cagcgcactg gaaaaccgtt cccgcatgga cagggcaaac atgaccgttt tcgccgtggt
      901 cctggccgtt gccctcgcag ccctggtcct cgccttcacc accatccggg cgatccgtcg
      961 ggccgttgac cgcatcctct ccgcatcaga ggccggcagg gaggggaatc tcacggtccg
     1021 gaccaccctg gacggaaccg atgagctcgc ggctgtgggc ggagccctcg acggaatgct
     1081 cgacagcctc aggaacgtgg tcaaagacat ccagcagaag gcggcggccc tttcatccct
     1141 ctcggaaacc acggcggcct cctgcgaaga agtgaccagc accaccaacg aggtggcgga
     1201 gagcaacaca cgcctcgcgg aagaggtaac caggggaaga accggcgccg gagaggcgtc
     1261 gaagatggtc caggagatga acaccatcat cctctcggcg aaggagctgg cgtcgagcgc
     1321 cgaccagaac tcccgccaga tggcgtccgc cgcggccagg gggaaggaaa cggtggccca
     1381 gtccatcggc cacatggaga acatccggaa cgccgtcggc gagacggaaa aactcatcac
     1441 ggaactcaac aactattctg cccggatcgg cgtggtgggc accaccatca cctccctcgc
     1501 cgaccagacc aacctcctcg ccctgaacgc tgccatcgag gcggcacggg ccggagaggc
     1561 ggggcgcggg tttgccgtag tggcggaaga agtccggaag ctggccgaac agtcccagca
     1621 gggtgcccgg gaagtggcgg agcttgtggg caagatcctg gaagggactg agtcggcggt
     1681 gaaatccatg gagacgagca ggaagggcgt tgaggaaggg gtgtccatcg cccacgtggc
     1741 cggagaagcc ctggagaaga tcatggaggc cacaaaaagc tccgtggagg atatacggcg
     1801 catcatcgcc gccacggaga aggaggcgga acagtcaggc catgttatcg ccctcattga
     1861 cgacacgacc tcggtcatgg agaacgcgga cgaacaggtc cagaacgtgg ccgcctccat
     1921 ggaggaaacc gcggcggcca tggagagcgt cgccaccggg gccaccgagg taagcgccac
     1981 cgctgaggac ctgcggaaga tcacagaacg gttcgtcgtg gacggcggca ccccgccccg
     2041 gggtctcgcc ctcagataaa gacgaaatct gaacggaaaa aggggccttc cggccccttt
     2101 ttctttttta atgccgatct cagtccagct cggctatgac gtcgatctcc accaggatat
     2161 cgaggagatc gcttcccacc gtcgttctca cggggaaggg ttccttgaag aattccctgt
     2221 agacttcgtt gaatgccctg aaatcctttg tgacctcctg aagatgggcg gtcactttca
     2281 ccacgtggtc catggaggcg cctgcggcct ccaggatggc ctgcacgttc ttcagggtct
     2341 gccgtgtctg ttcggcg
//
LOCUS       DAOTHD010000300         2354 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_53718_length_2354_cov_3.036973, whole genome shotgun sequence.
ACCESSION   DAOTHD010000300 DAOTHD010000000
VERSION     DAOTHD010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53718_length_2354_cov_3.036973"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..946)
                     /locus_tag="P5219_05970"
     CDS             complement(167..946)
                     /locus_tag="P5219_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HRX26336.1"
                     /translation="MRYYVVDAFAEKVFEGNPAGICVLDSWLPDGLMQNIAMENNLSE
                     TAFTVKEEKAYRLRWFTPGGEIDLCGHATLAAAYVLSRFKEPDAGEFSFETRSGRLTV
                     TRREDLFELDFPAYVLTSTPVTDAMAEAIGFRPTEAWSARDLVCVLDDERQVREAAPD
                     MARVRELDGLLLHLTARGKDHDCVSRSFAPKLNVPEDPVCGSGHCHIVPLWSRKLGKH
                     DLIARQASSRGGTLFCRDCGERVLLAGRAVLYSIAEIYPGE"
     gene            complement(969..1619)
                     /locus_tag="P5219_05975"
     CDS             complement(969..1619)
                     /locus_tag="P5219_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRX26337.1"
                     /translation="MKIRVLMIDDHRLFLAGLEDILAREDDFEVAGVAGSCREGMALA
                     LEKRPDVIILDLAMPETSGVEAARILSEALPGVRMAALTMHLDRQMVFEALKSGMTGY
                     ILKEATPEEFLFALRVIARGETWLSPKVATLVAKDFVRQGAQEGASEPALSEREREVL
                     HLLVKGAGTREIAGKLHISKSTVDTHRRNILDKLGCENVTCLTRYALRHGLVDLDE"
     gene            complement(1616..>2354)
                     /locus_tag="P5219_05980"
     CDS             complement(1616..>2354)
                     /locus_tag="P5219_05980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX26338.1"
                     /translation="IFLDMTDWKRAEEVLLEKEVLLTEYRQELRTLAARLSLAEERTR
                     REIAVKLHDRVGYSLVSLLSRLRALAEKHSPDDIAPAIEEVEGLLSDTRSLTLEISSP
                     LLYEVGLEPALASLGDRMLTPHGISVDFRECGPEANISMEIRILMYQMAQELLLNVIK
                     HAGAKKVILRVQRGNKRVRLLVEDDGAGFSSNTPKHWGKWGGMGLFSIREKLHSVGGE
                     IRVFSEKGKGSTVAIVAPLAFEGGTAQ"
BASE COUNT          421 a          725 c          703 g          505 t
ORIGIN      
        1 catctgagga attccctttc gcagtgggga tggaattcag cgatggtact gacggtgctt
       61 gcgatgcttc tctggatccg gtccggccga aagtagacgg ctgtttccct ccgaaggctg
      121 ccggagaaga tggccccgca gccttcggcg ttttccggat actgcgctat tctccgggat
      181 aaatctccgc aatggagtac aggacggccc tgccggcaag gaggaccctt tcgccgcagt
      241 cccggcagaa gagtgttccg ccccggctgg aagcctgccg ggcgatcagg tcgtgtttcc
      301 ccagcttccg gctccagaga gggacaatat gacaatgtcc tgatccgcag acaggatcct
      361 ccggcacgtt cagtttcggg gcaaagctgc gggatacgca gtcgtggtcc tttccccggg
      421 cggtcaggtg gaggagaaga ccgtccagct cccgaactct tgccatatcc ggcgcagctt
      481 cacggacctg ccgctcgtca tcaagcacgc agaccaggtc tctcgccgac caggcctcag
      541 tagggcggaa gccgatggct tccgccatgg catccgtgac cggtgttgag gttagaacat
      601 aggctggaaa gtccagttca aacaggtctt ccctgcgggt caccgtcagc ctgccgctcc
      661 gcgtttcaaa ggagaattcc ccggcatccg gttccttgaa cctggagaga acgtaggccg
      721 cggccagggt tgcatggccg cacaggtcga tctctccccc gggggtaaac caccgcagcc
      781 tgtaggcctt ctcctctttc accgtaaagg cggtttcgga caggttgttt tccatggcga
      841 tgttttgcat cagcccgtcc ggcagccagc tgtccaggac gcagattccc gcgggatttc
      901 cttcgaacac cttttccgca aacgcgtcaa ccacgtaata cctcattttc ttcccctttc
      961 ttcttccatt attcgtccag gtccaccagt ccgtgacgca gggcgtaacg ggtcaaacag
     1021 gtgacgttct cacagccaag cttgtcgagg atgttgcggc ggtgggtgtc cacagtgctc
     1081 ttgctgatgt ggagcttccc ggctatctcc cgtgttccgg cacccttcac aaggaggtgc
     1141 agtacctccc gctcccgttc cgagagggcg ggttcgctcg ctccttcctg ggctccctgc
     1201 cggacgaagt ctttcgccac gagggtggcg accttgggac tgagccatgt ttccccgcgg
     1261 gcaatcaccc ggagggcaaa gaggaattcc tcgggagtgg cttctttcag tatgtatcct
     1321 gtcatgccgc ttttcagggc ttcaaaaacc atctgtcggt caaggtgcat ggtgagggcc
     1381 gccatccgga cgccgggcag tgcctccgaa aggatgcggg ccgcctcaac gccgctggtt
     1441 tcgggcatgg caaggtcgag aatgataacg tcggggcgct tctcgagcgc cagcgccatg
     1501 ccttcccggc agcttccggc cacgcccgcc acctcgaaat catcctcccg ggcgagaatg
     1561 tcctcaagtc ctgcaagaaa gagcctgtgg tcgtcgatca tcaggacccg tatcttcatt
     1621 gcgccgttcc tccttcaaat gccagcgggg cgacgatggc cacggtggat cctttgccct
     1681 tctcggagaa aacccggatt tcgccgccca cggagtgaag tttttcccgt atgctgaaaa
     1741 gtcccatgcc gccccacttt ccccagtgtt tcggcgtgtt ggaggagaac ccggcaccgt
     1801 cgtcctccac gagcaggcgg acccgcttgt tgccgcgctg cacccggaga atcaccttct
     1861 ttgccccggc gtgcttgatt acgttcagca gcagctcctg ggccatctgg tacatgagaa
     1921 tgcggatttc catggaaatg ttcgcttcag ggccgcattc ccggaagtcg acggatatgc
     1981 cgtggggcgt cagcatccgg tcgccgaggg aagcgagggc aggttccagt ccgacttcgt
     2041 agagaagcgg cgagcttatc tccagtgtca gggaacgggt gtcggagagg agcccttcca
     2101 cttcctcgat ggccggggca atgtcatcgg gagaatgctt ttcggccaat gccctcagtc
     2161 gggagagcag ggagacgagg gaatagccta ccctgtcgtg gagttttacg gcgatctccc
     2221 ggcgggtccg ttcctccgcg agggaaagtc tggcagcgag ggtgcgcagt tcctggcggt
     2281 actccgtgag gaggacttcc ttttccagca ggacctcctc cgcccgcttc cagtcggtca
     2341 tgtcgaggaa gatc
//
LOCUS       DAOTHD010000301         2352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_53771_length_2352_cov_3.703091, whole genome shotgun sequence.
ACCESSION   DAOTHD010000301 DAOTHD010000000
VERSION     DAOTHD010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53771_length_2352_cov_3.703091"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="P5219_05985"
     CDS             <1..886
                     /locus_tag="P5219_05985"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRX26339.1"
                     /translation="VLAQGFVLMSGNLDLSIDAVLAFAPGMAILVSVKWFPGLLGNPW
                     TAILLTLSIGALLGLFNGFCVARLGMNAFMQTLSVSIILRGLVLFFIPLSIFPLDPVY
                     SFIGKARIVQLGNIPAAIPVTFLIFFIFHVLINYTVFGRNYLATGGNTRASFVAGINT
                     NRMIILGFMVAGILSAIAGMLTAGRQDSVSNTMGNGMTLLAFAGALLGGTSMSGGKGS
                     AFGMLGGAILLGMFSNALNLLGVKVTLIHAAQGTLIFLAILIDRFRVALRGSILRKEQ
                     VRKLHHSLNEQAENVTSY"
     gene            900..1958
                     /locus_tag="P5219_05990"
     CDS             900..1958
                     /locus_tag="P5219_05990"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRX26340.1"
                     /translation="MKRLFFCLAAAALLMVTISEGALAAGKQLTIGMVVKAPSAPYIQ
                     AFMKAAEDRAKSLGAEIMIRDGEGDSIKIMEIIDTFLAQGIDAFLLGGAVDLRTLVPG
                     IRKLNEANVPVAALDSSPEGGVVDFFLSFDLAQSSAKAAELFIEGIKNRNGGKVPEGV
                     VLEIVGDNADMFTHACTEGFNSVISKYPQLQVAQGEGKWNNTDSHAVTSDLITRFGEK
                     IVGIYVQTPDVMAPGAVAAIEAAGRNPADFGIAGICIGPEGIDLIKKGKMLGAVEQPA
                     YDGAALAVQYLVDKLKGNPVPKIGDTIVVEGAIWSPAKVIRNQWADDGAFVVLQGPLV
                     PQEISPDDPRLWENMLTK"
     gene            2002..>2352
                     /locus_tag="P5219_05995"
     CDS             2002..>2352
                     /locus_tag="P5219_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRX26341.1"
                     /translation="MISRKRVSRVVVLISALLVVLSVSGMAFAGQRLDRIMETKVLRV
                     GTPGDYRPFSMLDKASGKYEGHDVELAEFLASELGVAVEFVPTTWPKLMEDYLAGSFD
                     IAVGGITRSLARMLK"
BASE COUNT          518 a          613 c          677 g          544 t
ORIGIN      
        1 cgttctcgcc cagggattcg ttctcatgag cggaaacctg gacctttcca tcgatgccgt
       61 tctcgccttc gcgccgggca tggcgatcct ggtgtcggtc aagtggtttc cgggactgct
      121 cggcaacccc tggacggcca ttcttctcac cctgtccatc ggggccctgc tcgggctttt
      181 caacggattc tgcgtcgcca ggctcggaat gaacgccttc atgcagactc tttccgtctc
      241 catcattctc aggggactgg tactgttctt catccccctg tccattttcc ccctcgaccc
      301 ggtgtattcc ttcatcggga aggcacggat cgtccagctc ggcaatattc cagcggctat
      361 acccgtgacg tttttgatct ttttcatctt ccacgtgttg atcaactaca cggtcttcgg
      421 aagaaattac cttgccaccg gcggaaacac acgggcgagc ttcgtagccg gcatcaacac
      481 gaacaggatg atcatcctgg gattcatggt ggcaggaatt ctgtcggcca tagcgggaat
      541 gctcacggcg ggaagacagg attccgtgtc gaacaccatg ggaaacggaa tgacgctcct
      601 tgctttcgcg ggagcccttc ttggcggaac atccatgtcg ggaggcaagg gctcggcttt
      661 cggcatgctg ggaggggcaa ttttgctggg catgttttcc aacgccctga accttctcgg
      721 tgtcaaagtg accctcatcc acgccgccca ggggacactg attttccttg ccatcctcat
      781 tgacaggttc cgggtcgctc ttcggggctc cattctgagg aaggagcagg tccgaaaact
      841 ccatcattca ctgaacgaac aggctgaaaa tgtcacatca tactaaagga ggaattcaaa
      901 tgaagagatt gtttttttgt cttgcggcag cggcgcttct catggtgacc atttcggagg
      961 gagcactggc ggccggaaag cagctgacca tcggcatggt ggtcaaggct ccttcggcac
     1021 cgtatattca ggcgttcatg aaggctgctg aagacagggc aaaaagcctt ggcgccgaaa
     1081 taatgatacg ggacggagaa ggggattcca tcaagataat ggagattatc gacaccttcc
     1141 tggcccaggg aatcgatgcc tttcttctgg gcggggccgt tgacctcagg actcttgtgc
     1201 ccggcataag aaagctgaat gaagccaatg ttccggtggc agctctcgac tcttctccgg
     1261 aaggaggagt cgtggatttt ttcctttcct ttgacctcgc gcaatccagc gcaaaagctg
     1321 cggaactgtt catagaggga atcaagaaca ggaacggcgg aaaggttccc gaaggtgtag
     1381 tcctggagat agtaggcgac aacgccgaca tgtttaccca cgcctgcacg gaaggtttca
     1441 attcagtcat ctcgaagtat ccccagcttc aggtcgcaca aggggagggc aagtggaaca
     1501 ataccgattc tcatgctgtg acgtccgacc ttattacccg tttcggagaa aaaatagtcg
     1561 ggatctatgt tcagacccct gatgtgatgg ctcccggagc agtggccgca attgaagccg
     1621 caggcaggaa tccggcggat ttcggtattg ccggcatctg tataggcccg gaaggaatcg
     1681 acctgatcaa gaagggaaaa atgctcggag cggtggaaca gccagcctat gacggcgcag
     1741 cccttgcggt tcagtatctt gtggacaaac tgaagggaaa tcccgttccg aaaatcggag
     1801 acaccatcgt cgtggaaggc gcaatttggt ctccagcgaa agtcatcagg aatcaatggg
     1861 ctgacgacgg ggccttcgta gtgctccagg gaccgctcgt accccaggaa atcagccccg
     1921 atgatccgag gctctgggaa aatatgctga caaagtagcg tgatggagta taataggaca
     1981 aatttttctg gaggtgtgtt catgatatcc cgtaaaagag tgtcgagggt tgttgtcctc
     2041 atttccgcgc ttcttgttgt gctctcagtg tcgggaatgg cgttcgcagg gcagaggctg
     2101 gacaggatca tggagacgaa agtgctcagg gtggggaccc cgggagatta ccgtcccttt
     2161 tccatgctgg acaaggcatc ggggaaatat gaaggccacg acgtggagct tgcggagttt
     2221 cttgcctccg aactcggcgt cgcggtagag tttgtcccca ccacgtggcc gaagcttatg
     2281 gaggattatc ttgccggatc cttcgatatc gccgtgggtg gaatcaccag gagccttgcc
     2341 cggatgctga ag
//
LOCUS       DAOTHD010000302         2349 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_53859_length_2349_cov_3.312554, whole genome shotgun sequence.
ACCESSION   DAOTHD010000302 DAOTHD010000000
VERSION     DAOTHD010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53859_length_2349_cov_3.312554"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..1006
                     /locus_tag="P5219_06000"
     CDS             131..1006
                     /locus_tag="P5219_06000"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX26342.1"
                     /translation="MSIREIVIISGKGGTGKTTLTASLAAVAEEGKILADADVDAPNL
                     ALLLHPEETFREPFRGMSVAEVDRARCTGCGVCEESCRYDAIHVRDGKAVVDEGFCEG
                     CRVCLHVCPEQCISLKNFERGTIRRGDTALGPLWHARLNPGGENTGLMVALLRKEARK
                     EAEASGAQLIITDGPPGIGCPVTSSVTGADFAVIVSEPSRSALSDLRRAAELCGILSV
                     PFGIVVNRWNLSEELTDAIRTTCGDEGWSLLGTIPFDVKIAEAVGAGRIPAEEMGGDL
                     PELWRSIQKTGRLKR"
     gene            1003..1341
                     /locus_tag="P5219_06005"
     CDS             1003..1341
                     /locus_tag="P5219_06005"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HRX26343.1"
                     /translation="MIRIAVAASGKEETSQVADRFARAPFFIIADEEGKILEAFENDA
                     GAEQHGAASKALMTLSQKQVSVLLAPRMGPNALGFIRQANMDAFCAEGMTVREALDKY
                     KSASLEKINK"
     gene            1360..2124
                     /locus_tag="P5219_06010"
     CDS             1360..2124
                     /locus_tag="P5219_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRX26344.1"
                     /translation="MTLPPKSDKKQIKKIYAVGSGKGGVGKSSVTSLLAVSAARMGKK
                     VGILDADITGPSIPTFFGLSTFPFGNAEGIEPPKTSLGIKVISMNLLLDDPAKPVVWR
                     GPLLGNAVTQFFTDVQWNDLDVLFIDLPPGTADAVLTVMQTIPLDGFLLVTSPQEIAS
                     LVVRKAAKMAEMLSVPLVGVVENMSAFVCPDCGKRVELFGKSHSPALMDDLGASFWGS
                     LPLDPSLAEMADGGKIEAYENKEVFDILAQGLQGRI"
     gene            2160..>2349
                     /locus_tag="P5219_06015"
     CDS             2160..>2349
                     /locus_tag="P5219_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26345.1"
                     /translation="MQESIENRQDSVPGGAAAPGTAIEERSITEKLETVLGNLPPDRV
                     TLEEIRDLIGQDGLLLLSA"
BASE COUNT          508 a          649 c          754 g          438 t
ORIGIN      
        1 tgcttgcccg ctatcccttt tcccggtctg ccgcggaatg cggggccagg ggggaatcgc
       61 ccttcaaaac cgatgccgga tgggctgaaa tcacccgtac gctctggtcg aggatcgaaa
      121 ggagatactc atgagcatcc gggagatcgt gatcatcagc ggcaagggcg gcacgggaaa
      181 gacgacgctg acagcctctc tcgccgccgt ggcggaggag ggaaaaatac tcgccgatgc
      241 cgacgttgac gctcccaacc tcgctctcct gctccatccg gaggagacgt tcagagaacc
      301 cttccgcggc atgtctgttg ccgaagtcga ccgggcgcgg tgcacgggat gcggagtgtg
      361 cgaagagagc tgcaggtacg acgccattca cgtccgggac gggaaagccg tcgtggacga
      421 gggattctgc gagggatgca gggtatgcct tcatgtctgt cccgagcagt gcatttcgct
      481 gaaaaatttc gagcggggaa ccatacgccg gggggatact gctctgggcc ccctgtggca
      541 cgcccggctc aatcccggag gggagaacac ggggttgatg gtagcccttc tgcggaagga
      601 agcgaggaag gaagcggagg cctccggtgc gcagctcata ataaccgacg gacctccggg
      661 catcggctgt ccggtgacgt cgtcggtcac gggagccgat tttgcggtca ttgtctccga
      721 gccttcccgg tccgccctgt cggatctccg gcgggctgcg gaattatgcg gtatactgtc
      781 ggtgcccttc gggatagtgg tcaaccgctg gaacctctcg gaggagctca ccgatgcgat
      841 acggacaacg tgcggagatg aaggatggtc ccttctcgga acaataccct tcgacgtgaa
      901 gatcgcggag gctgttggtg ccggacggat acccgctgag gaaatggggg gggacctccc
      961 ggagttatgg cgttcaatac agaaaacagg gaggctgaaa cgatgatccg gatagcggtc
     1021 gccgcgtcag gaaaagaaga aacgtcccag gtagccgaca ggtttgcacg ggctcctttt
     1081 tttatcattg cggacgaaga aggaaaaatc cttgaggcgt ttgaaaacga cgccggggcc
     1141 gaacagcacg gcgccgcttc caaggctctc atgacgctgt cccagaagca ggtttcggta
     1201 ctgctcgccc cgaggatggg gccgaatgcc ctcgggttta tccggcaggc gaacatggat
     1261 gcgttctgtg ccgaaggaat gacggtgagg gaagccctgg acaagtataa aagcgcttcc
     1321 ctggaaaaaa taaacaagta gaaggagcgg acttcattca tgacattacc gccgaaaagc
     1381 gacaagaagc agattaaaaa gatatacgcc gtgggcagcg gaaagggagg cgtgggaaag
     1441 agctccgtca cgtcccttct cgccgtctcc gcggccagga tgggcaaaaa agtgggcatt
     1501 ctggacgccg acataaccgg gccgtccatt cccactttct tcgggctcag cacgttcccc
     1561 ttcgggaatg ccgagggcat cgagcctccg aagaccagcc tcggaatcaa ggtcatctcc
     1621 atgaacctcc tgctggacga tccggcgaag cccgtggtct ggcggggtcc ccttctgggg
     1681 aatgcggtga ctcagttctt taccgacgtg cagtggaatg acctggacgt tctcttcatc
     1741 gaccttcctc ccggaacggc cgatgcagtg ctcacagtaa tgcagaccat tccgctggac
     1801 ggcttccttc ttgtcacgtc tccccaggaa atcgcgtccc tggtggtccg gaaggcggcg
     1861 aagatggcgg agatgctctc cgttcccctc gtgggggtgg tggaaaacat gagcgccttt
     1921 gtctgcccgg actgcggcaa gagggtagag cttttcggca aaagccattc gcccgccctt
     1981 atggatgatc tcggcgcctc cttctggggc agccttcccc tcgacccctc cctggcggag
     2041 atggcggacg ggggaaaaat cgaagcatac gaaaacaaag aggtttttga tatacttgca
     2101 caggggctgc agggcagaat ctaggctcaa aaagtatgcc ccgggggagt aatgagggta
     2161 tgcaggagag cattgaaaac aggcaggact ccgttcccgg tggcgcagcg gcaccgggaa
     2221 cggctattga agagcggtcg atcaccgaaa agcttgagac cgtattgggg aacctccccc
     2281 cggacagggt caccctggag gaaatacggg acctcatcgg ccaggacggc ctgcttctgc
     2341 tgtcggcct
//
LOCUS       DAOTHD010000303         2345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_54008_length_2345_cov_2.955459, whole genome shotgun sequence.
ACCESSION   DAOTHD010000303 DAOTHD010000000
VERSION     DAOTHD010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54008_length_2345_cov_2.955459"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="P5219_06020"
     CDS             <1..1129
                     /locus_tag="P5219_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRX26346.1"
                     /translation="RSLHEIPELGLETPKTAAFIVQELKKMGAEDIREKVGGWGVTAL
                     VRGALPGKTLAVRADCDALPIKEETGLPFASKNGLMHACGHDAHTAMALGAAKLLLAR
                     KSELAGTVKFIFQPAEENVLGAKAMIDDGALENPAVDALIGLHTGCLWKDIGFGEIGV
                     RYGSLMAAVDRFIITFKGKGGHGATPHLAVDPVTMTSTAVLELQTILSRELSPLDPAV
                     LTIGRIEGGRAFNIIAESCTIEGTVRTLSPATRAFVEERIRAIASRVAEGMRGEAEVK
                     YEAGPPALINDTEFTKKFQAFAAGVAGADKVKEISEPTMGGEDVAFFLEKVPGTFFVH
                     GSCNPDKGQVYPHHNPKFDIDEDALPLGSALFAGFALTWQK"
     gene            1237..2331
                     /locus_tag="P5219_06025"
     CDS             1237..2331
                     /locus_tag="P5219_06025"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase [NAD+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HRX26347.1"
                     /translation="MSRIMIAPGKYIQGYGELKRLKDHIGAMGSTFLVLISRGGLGRL
                     ESTLKAGFRGNESALVFETFNGECSKTEIDRIRAVCRDKKCDAVIGVGGGKILDTAKA
                     VAYYEKTPVIIVPTIASTDAPCSALSVIYTDEGVFSEYLVLPKNPDAVLMDTEVIVNA
                     PARLLVAGMGDALATYFEARGCIAADKTNMPGGHATKAALALAELCYDTLLEDGLKAL
                     LAVETGSCTKAVENIVEANTFLSGLGFESGGLGAAHAVHNGFTVLEECHGLYHGEKVA
                     FGTLVQLVLENAPEEELEEVMGFCASVGLPVTLEEMGVKEVDRARIMDVARAACAPGE
                     TIHNMPFAVSPEDVCSAILAADALGRHFLA"
BASE COUNT          478 a          764 c          729 g          374 t
ORIGIN      
        1 gcgctcgctt cacgaaattc cggaactggg tcttgaaaca ccgaaaaccg ccgcctttat
       61 agtacaggaa ctgaagaaaa tgggcgcgga ggacattcgg gaaaaagtgg gagggtgggg
      121 cgtgacagcc ctcgttcggg gagcccttcc cggaaagacg ctggccgtgc gggccgactg
      181 cgacgccctt cccatcaagg aagagacggg gcttcccttc gcctcgaaga acgggctcat
      241 gcatgcctgc ggccacgacg cccacaccgc catggccctg ggagccgcga agctgcttct
      301 cgctcgaaag agcgagcttg ccggcacggt gaagttcatc ttccagccgg cggaagaaaa
      361 cgtcctcgga gcgaaggcca tgattgacga cggcgccctt gaaaaccctg ccgtggacgc
      421 cctcatcggc ctccacaccg gctgcctctg gaaagacatc ggcttcggcg agatcggcgt
      481 ccggtacggg tccctcatgg cggcggtgga ccggttcatc atcaccttca agggcaaggg
      541 cggccacggg gccacacccc atcttgcggt ggacccggtc accatgacct ccacggcggt
      601 gctggaactc cagaccatcc tcagccggga gctttcgccc ctcgacccgg cggtgctcac
      661 catcgggcgc atcgagggcg gcagggcatt caacatcatc gccgagagct gcaccatcga
      721 gggaaccgtc aggaccctca gccccgccac ccgggccttc gtggaggagc ggatccgagc
      781 catcgcgtcc agggtcgccg agggaatgcg gggcgaggcg gaagtgaagt acgaagccgg
      841 gcctcccgcc ctgatcaacg acacggagtt cacgaagaag ttccaggcct tcgccgccgg
      901 cgtcgccggg gcggacaagg tgaaggaaat ctccgagccc accatgggag gggaggacgt
      961 ggccttcttc ctggaaaaag tgccgggcac cttcttcgtc cacggatcgt gcaacccgga
     1021 caaggggcag gtctaccccc accacaaccc gaaattcgac atcgacgagg atgcccttcc
     1081 cctgggaagc gccctcttcg ccggtttcgc cctcacctgg cagaagtagc ggaaagagaa
     1141 aagagaataa ctcccgcccc cggatttcgt tcctccgggg gctttttggt atgatataat
     1201 agtgaacgaa atatcatatc aagcgaggtg ttgttcatgt ccagaatcat gatcgctccg
     1261 ggaaagtaca tccagggcta cggagaactg aagcggctga aggaccacat cggcgccatg
     1321 gggagcactt ttctggtgct catcagcagg ggaggactgg gccgtcttga gagcaccctc
     1381 aaggccggct tccgggggaa cgaatccgcc cttgtgttcg agaccttcaa cggagaatgt
     1441 tcaaagaccg aaatcgaccg gatccgggcc gtctgccggg acaagaagtg cgacgccgtt
     1501 atcggcgtgg gcggagggaa gatcctcgac accgccaagg ccgtggccta ctatgaaaaa
     1561 acgcccgtga tcatcgtccc caccatagcg tccaccgacg cgccctgcag cgccctgtcg
     1621 gtgatctaca cggacgaagg cgtcttcagt gaatacctcg tcctgccgaa gaaccccgac
     1681 gcggtgctta tggacaccga ggtcatcgtc aacgcccccg ccaggctgct cgtcgccggc
     1741 atgggcgacg ccctggccac gtacttcgag gcccggggct gcatcgccgc tgacaagacc
     1801 aacatgcccg gcggacacgc cacaaaggct gccctcgccc tggcggaact ctgctacgac
     1861 accctcctcg aggacggcct caaggccctt ctcgccgtgg aaaccgggag ctgcacgaag
     1921 gccgtggaaa acatcgtgga ggccaacacc ttcctcagcg gcctcggctt cgaaagcggc
     1981 gggctgggcg ccgcccacgc cgtccacaac ggcttcaccg tgctggagga atgccacgga
     2041 ctctaccacg gggagaaggt cgccttcggg acgctggtgc agcttgtcct cgagaacgcc
     2101 cccgaggagg agctggagga agtgatgggc ttctgcgcct ctgtgggact gcccgtgacc
     2161 ctcgaggaaa tgggcgtgaa ggaagtggac cgggcccgga tcatggacgt ggccagggcc
     2221 gcctgcgccc ccggcgagac catccacaac atgcccttcg ccgtttcacc tgaggacgtg
     2281 tgctccgcca ttctcgccgc cgacgccctc ggacggcact tcctggccta agctatcctg
     2341 gaacc
//
LOCUS       DAOTHD010000304         2338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_54198_length_2338_cov_3.666667, whole genome shotgun sequence.
ACCESSION   DAOTHD010000304 DAOTHD010000000
VERSION     DAOTHD010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54198_length_2338_cov_3.666667"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="P5219_06030"
     CDS             <1..349
                     /locus_tag="P5219_06030"
                     /inference="COORDINATES: protein motif:HMM:NF020672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HRX26348.1"
                     /translation="RERGFQPPLISEAREKLALSEDAFSILLNGMRKTGMVSIVSGEF
                     ILSAEVENELLRILLREKDGITLARVRDITGSSRKFILPLLEYLDAKGYTRRAGEKRV
                     LLASKLPGEITGP"
     gene            457..693
                     /locus_tag="P5219_06035"
     CDS             457..693
                     /locus_tag="P5219_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HRX26349.1"
                     /translation="MATITSIREQVALYKGLKVRYRTAKGRRKVEERQGVILETYPNL
                     FTLYVESQDSKVSFSYAELLTREVELELLSGSRN"
     gene            1010..1627
                     /locus_tag="P5219_06040"
     CDS             1010..1627
                     /locus_tag="P5219_06040"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26350.1"
                     /translation="MDLPPLHISLVKNIPPGSGLGGGSGNAAALLSWLNRMGGRNSLL
                     PEEFGSDVPFLFSDALWASVSGRGEIITPVASPPETPVVLVAVPSWKMSTQTAFSLID
                     EARGGRFPLNGGEAERERDDLLDGLREQRHVGLLPNDFLPVLLNLYPRYSELFSAFSD
                     YGAAGWGITGSGSACFGLFYKKNGLGSFLRDMTRYDWIRKIFCLE"
     gene            1635..>2338
                     /locus_tag="P5219_06045"
     CDS             1635..>2338
                     /locus_tag="P5219_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRX26351.1"
                     /translation="MKGQRTERLIRTAARFLTCPSKQLSLTSLAEDFLVSKTVISDDV
                     AIIDEALSREGMGGISVDRGRTGGASFVPRMAAAVKDSLLREIMDILNCEDRFLPGGL
                     VYYSDILFNPHYALRLGYAMASLFSETRPDVVMTSEVKGIPLAIFTAHAMGIPLAVCR
                     FRNRASDGSAVTVHYPTKNGDVKAMYMGTKQLGKGKRVLIVDDFMRGGSTAAGMLLVA
                     KEFGAEVAGTGIFMVSS"
BASE COUNT          548 a          602 c          654 g          534 t
ORIGIN      
        1 cagggaaagg gggtttcagc ctcccctcat ttccgaggcg agagagaagc tcgcactctc
       61 cgaggatgcc ttttcaatcc tgctcaacgg catgcgcaaa acaggcatgg tttccattgt
      121 atccggggag tttattcttt cagcggaggt ggagaatgaa ctcctccgta tactcctcag
      181 ggaaaaagac gggatcaccc tggccagggt acgggatatc acaggcagtt cgagaaaatt
      241 cattcttccc ctgctcgagt atctcgatgc gaagggctat accaggaggg caggagaaaa
      301 aagagttctt ctcgcctcaa agcttccggg ggagattacc ggtccctgag ataccgggcc
      361 catggcccct ggtcggcatg agccccctga catttgatat tttttcatat ttatgataag
      421 attcatgcaa tatttgcagt gctgggagtg acccgaatgg cgacaataac ttcgatccga
      481 gaacaggttg cgttgtacaa gggattgaaa gtccggtacc ggacggccaa ggggcgccga
      541 aaagttgaag agcgccaggg agtcatcctg gaaacctatc cgaacctctt tactctttac
      601 gtcgaatctc aggatagcaa ggtatccttc agctatgcgg agcttctgac ccgggaggtc
      661 gaactggaac ttctttccgg aagcaggaac tgatcccttt tgaacgtcaa aaaaagacgc
      721 cccgacaccc gtgcgtgccg gggcatcttt tttgctctca caggaggagg tatgtatgtc
      781 cttttttgtc caatcggacg caaaaatcaa tatttcgctt cggatcaccg gaaaacgccc
      841 tgacggatac cacgaactca gatcagtttt tttgcgactt tcagcgttag aatcattgac
      901 aattaccccg caatatgatc ataatgtccg gaacagcctg tccgttcacg gagagaccgt
      961 tcaggggaaa aacatacttg atgaagttct ggcccgtgcc gggcagacca tggatcttcc
     1021 gccccttcac ataagccttg tgaagaatat accgccggga agcggtctgg gcggagggag
     1081 cggaaacgca gcggccctgc tttcctggct gaaccgcatg gggggacgaa atagcctcct
     1141 gccggaagag ttcggatccg acgttccctt ccttttcagc gatgctctgt gggcctcggt
     1201 gagcggaaga ggagaaatca tcacacccgt tgcctcacca cctgaaacgc ctgtggttct
     1261 tgttgcggtt ccgtcctgga aaatgtccac tcaaactgca ttttccctga ttgatgaagc
     1321 acggggagga cgttttcctc tgaatggcgg cgaggcggaa agggagaggg acgaccttct
     1381 tgacggattg cgggaacaac ggcatgttgg tcttcttcca aacgattttt tgccggtgct
     1441 tctgaatttg tacccccggt attcggagct tttcagcgcc ttcagcgatt acggggcggc
     1501 aggctgggga attacgggaa gcggaagcgc ttgtttcggc cttttctaca aaaaaaacgg
     1561 cctcggaagt tttttgcggg acatgacgag atacgactgg atacggaaaa ttttctgcct
     1621 ggagtgatga cgaaatgaaa ggacaaagaa cggaaaggct tatccgcaca gcggccaggt
     1681 ttttaacctg cccatcaaag cagctttcac tcacatccct cgccgaggac ttccttgttt
     1741 cgaagacggt catcagcgat gatgtggcga tcatcgacga agccctttcc cgggaaggaa
     1801 tgggaggcat cagcgtcgac aggggaagaa cgggcggcgc atccttcgtt ccgaggatgg
     1861 ctgcggcggt aaaggattcc ctcctcaggg aaatcatgga catcctcaac tgcgaagacc
     1921 gttttcttcc aggaggactt gtttattaca gcgatatcct tttcaacccc cactacgccc
     1981 tccgtctcgg atacgccatg gcgtccctat tcagcgagac gaggcccgac gtggtcatga
     2041 catccgaagt caagggaatt cctctcgcca tttttaccgc tcatgccatg ggtattcccc
     2101 tggcggtttg ccgtttccgg aacagggcga gcgacggctc ggcggttacc gtccattacc
     2161 ccacgaagaa cggcgacgtg aaagccatgt acatgggaac caagcagctg ggaaagggga
     2221 agcgggttct catcgtggac gacttcatgc gcggcggaag tacggcggcc ggaatgctcc
     2281 tggtggccaa ggaatttgga gcggaagttg cgggaacggg aatcttcatg gtttcctc
//
LOCUS       DAOTHD010000305         2338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_54210_length_2338_cov_2.666229, whole genome shotgun sequence.
ACCESSION   DAOTHD010000305 DAOTHD010000000
VERSION     DAOTHD010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54210_length_2338_cov_2.666229"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..463)
                     /locus_tag="P5219_06050"
     CDS             complement(29..463)
                     /locus_tag="P5219_06050"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HRX26352.1"
                     /translation="MRTNRCRYRVAKLCKLLEVSRSGYYAWEKRPESSRKKENEELLA
                     LMKKLHGKHRGVYGSRKMKKALEKEGWKVNHKRVERLMKAGGLRSRLSVKYKATTNSK
                     HTLPVAENLLNRQFTAAKPGEKMVSDITYIRTDEGWLYAAAV"
     gene            complement(478..765)
                     /locus_tag="P5219_06055"
     CDS             complement(478..765)
                     /locus_tag="P5219_06055"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRX26353.1"
                     /translation="MGKYYTPDFKLEAVKRVQRTGEPVSKVAEELGINPNTLHGWMKR
                     IREHPEGPFPGSGKLSPEDDRLRKLERENRNLREEVEILKKAAVYFAKNQK"
     gene            932..>2338
                     /gene="istA"
                     /locus_tag="P5219_06060"
     CDS             932..>2338
                     /gene="istA"
                     /locus_tag="P5219_06060"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HRX26354.1"
                     /translation="MLKVDQYEYIRTAHRVYGKNVSEIARETGHSRNTVKKVLRGEFT
                     GYSPRKTQPSPVLGPYHEIINQWLEEDIESPKKQRHTAKRIYTRLVSEYGFTGSETTV
                     RHHVASVKRSLGLKSSEAFVPLEPSPRGEAEVDWGSATVLLGGEAVRVKIFCMRSKYS
                     GNPFVRLYPCERQQAFFDGLSRGFLYYGGVFPVMVFDNLTAAVGKVLLGKERKEQESF
                     VRFRSWYTFESRFCSPGRGNEKGGVEGLVGFARRNFLVPLPRGENLESINDRLLEECI
                     AYGSHRITGREGTVRELHDAEKRNLIPLPRHPYGNEQTVPVKADKYATVMVDKNRYSV
                     PASYAGRPLRALLTVDDVAVYSGEKRLAVHGRKYGNNHWVLEADHYLELLRERPGAFR
                     DARPLSEWKKTWSGSLNSLLERFQERQGENRGIKEFIDVLLLTRNYGQKRVEAAALRA
                     LENGLGSAAGIRHLLESAE"
BASE COUNT          502 a          706 c          628 g          502 t
ORIGIN      
        1 gcctaactct ttaatctgcg ggaagttatc aaactgctgc ggcatatagc cacccttcgt
       61 ccgtccggat atacgttata tccgatacca tcttttcccc aggcttcgcc gccgtgaatt
      121 gacggttcaa caggttctcc gccaccggca gggtgtgctt cgagttcgtc gtcgccttgt
      181 atttcacaga gagccgggac cgaagccctc cggccttcat cagccgctct actctcttgt
      241 gatttacttt ccagccctcc ttttccaggg ctttcttcat cttccggctt ccatagactc
      301 ccctgtgctt cccatggagt ttcttcatca gcgccagcag ttcttcattc tctttcttcc
      361 gtgaactctc cgggcgtttt tcccatgcgt aatatccgct cctggaaacc tcgagcagtt
      421 tacacagctt cgccacccgg tatctgcagc ggttggtgcg gataaactcg aacctcttta
      481 tttctggttc tttgcgaagt acaccgccgc tttttttagg atttctacct cttcacgaag
      541 atttcggttt tcccgctcca gctttctcag ccggtcatct tcaggcgaca gtttcccgct
      601 gcccgggaac ggcccttcgg gatgctcacg gatgcgcttc atccatccgt gaagggtgtt
      661 cgggttgatc ccgagctctt ccgctacctt cgaaaccggc tcccctgtcc tctgtaccct
      721 ctttaccgct tcaagcttga aatccggcgt gtaatatttg cccatcttct gcagcccctc
      781 tctgatctcc agtatatcgt ctctttggtt ttggtattct aaaattgatc actttttgag
      841 ggaacgctag ccgaaaattg accacctgac catcgctccg ggtatcatcg tctgaaacgc
      901 acaccacgac gatgaaaccg gaggagaaag gatgctcaag gtggaccaat acgaatacat
      961 acggaccgct caccgggtat acggcaagaa cgtgagcgaa atagccaggg aaaccgggca
     1021 ttcccgaaac accgtgaaga aagttcttcg gggtgaattt acaggctatt ccccaagaaa
     1081 gacccagcct tctccggtac tcggccccta tcacgaaatc attaaccaat ggctggaaga
     1141 ggacattgaa agcccgaaga agcaacgcca tacggcaaaa cggatatata cccgcctggt
     1201 gagcgaatac gggtttaccg gaagcgaaac gacggtgcgc caccacgtgg catcggtcaa
     1261 gaggagcctg ggcctgaaaa gcagcgaagc ctttgtgcct ctggagccct ctccgagggg
     1321 agaggccgag gtggactggg ggagcgccac ggtcctcctc ggcggagagg ccgtacgggt
     1381 gaaaatcttc tgcatgagat cgaagtactc ggggaacccc tttgtccgtc tctacccctg
     1441 tgaacggcag caggcgtttt tcgacggact gagccgggga tttctctatt acggaggcgt
     1501 ctttcccgta atggtctttg acaacctgac ggcggcggtg ggaaaagtgc ttctcggaaa
     1561 agagaggaaa gaacaggaaa gctttgtccg gttccgctcc tggtacacct tcgagagccg
     1621 tttctgctct cccggccggg gaaacgagaa gggcggggtg gagggactcg tgggattcgc
     1681 ccgccggaac ttcctggttc ccctcccccg gggagaaaac ctggagagca tcaacgacag
     1741 gctgctggaa gaatgcatcg cctacggctc ccacaggata accggccggg aggggaccgt
     1801 acgggaactg cacgatgcgg aaaagaggaa cctcatcccc cttccccgcc atccctacgg
     1861 aaacgaacag accgtcccgg tgaaggcgga caagtacgcc accgtcatgg tggacaagaa
     1921 ccggtactcg gtccccgcct cctacgccgg ccggcccctc agggcactgc tcaccgtgga
     1981 cgatgtcgcg gtctacagcg gagagaagcg gctcgccgtc cacggaagaa agtacggaaa
     2041 caaccactgg gttctcgagg cggaccacta cctcgagctc ctccgggaac gtcccggagc
     2101 cttccgggac gcccgccccc tttcggaatg gaaaaagaca tggagcggct ccctgaactc
     2161 ccttctggaa cggttccagg aacggcaggg agaaaaccgg ggaatcaagg aattcatcga
     2221 cgtcctgctc ctcaccagga actacgggca aaagcgggtg gaagccgccg ccttgcgggc
     2281 cctggaaaac ggactgggca gcgccgccgg aatccgccat ctcctcgagt cggccgaa
//
LOCUS       DAOTHD010000306         2337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_54245_length_2337_cov_2.418054, whole genome shotgun sequence.
ACCESSION   DAOTHD010000306 DAOTHD010000000
VERSION     DAOTHD010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54245_length_2337_cov_2.418054"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..1130
                     /locus_tag="P5219_06065"
     CDS             177..1130
                     /locus_tag="P5219_06065"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX26355.1"
                     /translation="MRKLFFWIFACIAVIAAALPPAAFADTAGDAYQRGQIAWKNQDY
                     ETAFREYRTAGDLGHLDAQILLAINLSASPYREDFTESRKWWAKAAGQGNSSSMVALG
                     DSYRHGDLDGNFVDYKEAVKWYRTASNSKNTEHRGDASLALGEMYEAGQGTAANPSEA
                     MKFYKKAADCGNGEAKFRIGLLYETGKGVGRNYAKAREWYTAAAGDWVSAAMYRVGYL
                     HEKGLGVKANMKEAEKWYRKAADAGDSWSQLYFGDKCEHGRGVPVDLISAHVWYNLAS
                     KGMGSEELWKKAKSAKERVALKLSAEELARAEKMAREWRPR"
     gene            1363..>2337
                     /locus_tag="P5219_06070"
     CDS             1363..>2337
                     /locus_tag="P5219_06070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRX26356.1"
                     /translation="MKRQAVRAITRIVLPYLIFAGLWIFFSDRLLEYMNLAPEDLTRL
                     SIYKGMAFVAVTAVLLTSLLLAESRAREITLAARMQAQEKLERNERRLRHLFSVSPSI
                     VYTLNPDDHSITWVSRNVTSLLGYPPEEVLRPGFWKAIIHPEDIPPVRDGARTAIRAG
                     KGVREYRLFRKNGEAIWVHDDLLVLADEENNPKEIICALTDITERKRSEQERIRLQVA
                     EQASRAKSAFVAHMSHEIRTPLNAILGFARMLERDPALTAGQAERVRTIIHSGNHLLS
                     LVSNILDFSKIESGKLELAVEGFNLHDLLDDVAAFFRPQAEARGLAFRY"
BASE COUNT          540 a          666 c          702 g          429 t
ORIGIN      
        1 tcagtatagg ggaagtgccg cccggccggt accggagaaa taaggatata ttcaggcagg
       61 cccatccggt gaaaaaaccg gatgcgcctt ttcggcgcgg ggagtacaat gcagccatgt
      121 acaggaaatg tcccggcagc tgaacctgaa ccgacatctc agggaggtga actcagttga
      181 gaaagctgtt cttctggatc ttcgcgtgca ttgcggtcat agcggcggcg ctgccccccg
      241 cagcctttgc cgacactgcc ggggacgcct accaaagagg gcagatcgcg tggaagaacc
      301 aggactacga aactgcattc cgtgaataca ggacagcggg agatctgggg catcttgatg
      361 cccagattct gctggcaata aacctctccg cttcccctta ccgggaggat ttcaccgaat
      421 cgaggaaatg gtgggcaaaa gctgcggggc agggtaattc cagttccatg gtcgccctcg
      481 gcgacagcta caggcacggg gatctcgacg gaaactttgt tgactacaag gaagcggtaa
      541 agtggtaccg cactgcatca aattcaaaga acactgagca ccggggcgat gcctctctcg
      601 ccctggggga gatgtacgaa gccgggcagg gaacggcggc gaatccttcc gaagccatga
      661 aattctacaa aaaggcggcg gattgcggga atggcgaggc aaaattccgg atcggccttt
      721 tgtatgaaac cggaaaagga gtcgggcgga attatgcgaa agcgcgggag tggtacaccg
      781 ccgctgcagg ggactgggta agcgcggcca tgtaccgcgt cgggtatctc cacgaaaaag
      841 gcctgggcgt gaaggcaaac atgaaagaag ccgaaaaatg gtaccggaag gctgccgatg
      901 ccggagacag ctggtcccag ttgtacttcg gcgataaatg cgaacatggc aggggcgttc
      961 ccgtcgacct tatctccgcc catgtctggt acaaccttgc ctcgaaaggc atggggagcg
     1021 aagaactctg gaagaaggca aaatcagcaa aggaaagggt tgcgctgaag ctctccgcgg
     1081 aagagctcgc aagggctgag aagatggccc gggaatggag gccgcggtaa cagcggatgg
     1141 aatcccaagc cttgcatgcc cctcaaggct tccacggaac cctctgttcg tccggcaccc
     1201 cttccggaat atgcggtagg ggtgcttttt tatcccccct ccggtttgac tttttcattg
     1261 acaatgggag agaatcgtat aatatcttca gaacaaaatt ctgccgggtt tacgggagaa
     1321 cctcttccgg ctcccgcccg gcggccgtgc cggggtgagg ccatgaaacg tcaggctgtc
     1381 cgtgccatca cgagaatcgt tcttccctat ctcatttttg ccggcctgtg gattttcttt
     1441 tcagaccggc tccttgagta catgaacctt gcccccgaag acctgacccg tctgtccatc
     1501 tacaagggca tggccttcgt ggcggtgacg gcggtccttc tcacgtccct cctcctggcg
     1561 gagagcaggg ccagggaaat caccctcgcc gcccgcatgc aggcccagga aaaactggag
     1621 cggaacgagc ggcggctgcg gcatcttttt tccgtgagcc cttccatcgt gtacaccctc
     1681 aaccccgacg accattccat cacctgggtc agccggaacg taacctccct gctcggctac
     1741 ccgccggagg aagtcctgcg gccaggcttc tggaaggcca tcatccaccc ggaagacatt
     1801 ccgccggtac gggatggagc gcggacggcc atcagggcgg gaaaaggggt ccgggaatac
     1861 cggctgttca gaaagaacgg agaagcgatc tgggttcacg acgacctcct ggtgctggca
     1921 gacgaagaga acaatccaaa ggaaatcatc tgtgccctca cggacattac cgaaaggaag
     1981 cggagcgagc aggagcgcat acggctccag gtggcggagc aggccagcag ggccaagagc
     2041 gccttcgtcg cccacatgag ccacgaaatc cgcacgcctc tcaacgcgat cctcggtttc
     2101 gcccggatgc tggagcggga tcccgccctc acggcaggcc aggcggaacg ggtcaggacc
     2161 ataatccaca gcggaaacca ccttctctct ctcgtcagca acatcctcga tttttcgaaa
     2221 atcgaatcgg gaaaactgga gctcgccgtc gaaggattca atctccatga cctgcttgac
     2281 gacgttgcgg cgttcttccg tccccaggca gaggccaggg gactggcttt ccggtac
//
LOCUS       DAOTHD010000307         2336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_54273_length_2336_cov_3.363875, whole genome shotgun sequence.
ACCESSION   DAOTHD010000307 DAOTHD010000000
VERSION     DAOTHD010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54273_length_2336_cov_3.363875"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /gene="glyA"
                     /locus_tag="P5219_06075"
     CDS             <1..1022
                     /gene="glyA"
                     /locus_tag="P5219_06075"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRX26357.1"
                     /translation="ANVQPHSGVNANLAVFMAMLEPGDVILGMDLRHGGHLSHGTAVN
                     ISGKFFRAFSYGVDRETGRIDYDEAERLALEHRPKMIIAGGSAYPRVIDFARFAEIAG
                     KVGAYFLVDMAHIAGLVAAGVHPSPVPHAHFVTFTTTKTMRGARGGNILCRKEFASRI
                     DKAIFPGIQGGPIPQIMAGKALTFRLAMTEDFRNYGRRTVENAARFASVLTARGFRLV
                     SGGTDNHLMLVDLRSKGLTGREAEELLHAAGITVNMNLIPFDPEKPTVTSGIRIGLAG
                     VTSRGFTAADAEETARITADLLEKKPAEGLSYRERVLRLCLDHPLYMTREELAASPFL
                     TRDLL"
     gene            1307..>2336
                     /locus_tag="P5219_06080"
     CDS             1307..>2336
                     /locus_tag="P5219_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HRX26358.1"
                     /translation="MRSEEVYAKSLDLHRAKKGKVAIESKMPVNSMADLALAYTPGVA
                     EPCREIGRNRDSIYEVTSKANMVAVITDGTAVLGLGDIGPDAALPVMEGKSILFKRFA
                     DIDAFPICINSKDPDEIVRTSSLILSSFGGVNLEDIAAPRCFEIERKLQEIADIPIFH
                     DDQHGTAVIVLSGLLNALKVVGKKLPDVKIVMSGAGAAGTAICKFLMSAGARNVIMCD
                     RGGAIYKGRTEHMNWAKEELAELTNPNGEKGSVADAMKGADVFLGLSGPGIIDQEMVK
                     TMAPKAVIFAMANPTPEIYPDEAIAAGAAVVATGRSDFPNQINNCLGFPGIFRGALDV
                     RASVINEEM"
BASE COUNT          487 a          764 c          681 g          404 t
ORIGIN      
        1 acgccaacgt ccagccccat tccggggtga acgcgaacct cgccgtgttc atggccatgc
       61 tcgagcccgg tgacgtcatc ctcggcatgg acctccggca cgggggacac ctgtcccacg
      121 ggacggcggt gaacatctcc ggaaagttct tccgggcctt cagctacggc gtggacaggg
      181 agacgggccg gatcgactac gacgaggccg agcggctcgc cctggaacac cggccgaaga
      241 tgatcatcgc cggagggagc gcctaccccc gggtcatcga cttcgcccgg ttcgcggaga
      301 tcgccggaaa ggtcggggcc tatttcctgg tggacatggc ccatatcgca ggcctcgtgg
      361 cggcgggagt ccatcccagc cccgtccccc acgcccattt cgtcaccttc accaccacga
      421 agaccatgcg gggcgcccgg ggcggcaaca ttctctgccg gaaggaattc gcctccagaa
      481 tcgacaaggc catcttcccc ggcatccagg gtggccccat cccccagatc atggcgggga
      541 aggccctgac cttcaggctc gccatgacgg aggacttccg gaactacggc cggcggaccg
      601 tggagaacgc cgcccggttc gcctcggtcc tcacggcccg gggcttccgc ctcgtttcgg
      661 gcggcacgga caaccacctg atgctcgtgg atctccgcag caagggcctc acgggcaggg
      721 aagccgaaga gctcctccat gccgccggaa tcacggtgaa catgaacctc atccccttcg
      781 accctgaaaa gcccaccgtc accagcggga tccgcatcgg cctcgcaggc gtcaccagcc
      841 ggggcttcac cgccgccgac gcggaggaaa cggcccgcat caccgccgac ctgctggaaa
      901 aaaagcccgc cgaaggcctc tcttacaggg aaagggtcct ccgcctctgc ctggaccacc
      961 ccctgtacat gacccgggag gagctggcgg cgtcgccctt cctcaccagg gacctcctgt
     1021 agagcggcgg cactccctct gccggcaatc cgcggattga gggggaaaaa agtttgacaa
     1081 atttgctgat tttttactat aatctcaccg gaacttattg gtttccatgg tataccaaat
     1141 atgtttcctg atcatgccgt gcccggaatt tccttgcctc gccacaccat gaatgggacg
     1201 aaaatacacg gcggtttccc gaggaaagtg ttagaatact ttttgtgacg ttctgaagtt
     1261 tgacgattta ccgcacattt tctatttccg cgaaggaggt tctttcatga gaagcgagga
     1321 agtctacgca aagtccctag acctgcacag ggcgaaaaag ggcaaggtgg ccatcgaaag
     1381 caagatgccg gtgaactcca tggccgacct ggccctggct tatactcccg gcgtggccga
     1441 gccctgcagg gagatcggac ggaacaggga ttccatctac gaagtgacgt ccaaggccaa
     1501 catggtggcc gtcatcaccg acggcaccgc ggttctcggc ctcggcgaca tcggccccga
     1561 cgcggccctc cctgtcatgg aggggaagtc gatcctcttc aagcgcttcg cggacattga
     1621 cgccttcccc atctgcatca attccaagga cccggacgag atcgtcagaa cctcatccct
     1681 catcctctcc tccttcggcg gagtgaacct cgaggacatc gccgccccga ggtgtttcga
     1741 aattgaaagg aagctgcagg aaatagccga tatccccatc ttccacgacg accagcacgg
     1801 aacggccgtc atcgtcctct ccgggctcct gaacgccctg aaggttgttg gaaagaagct
     1861 gcctgacgtg aaaatcgtca tgagcggcgc cggcgccgcc ggcacggcca tctgcaaatt
     1921 cctgatgtcg gcaggagccc ggaacgtcat catgtgtgac cggggcggcg caatctacaa
     1981 gggacgcacg gagcacatga actgggcaaa ggaagaactc gcggaactca cgaaccccaa
     2041 cggagaaaaa gggtccgtgg ccgacgcgat gaagggcgcc gatgtgttcc tcgggctctc
     2101 cgggccgggg atcatcgacc aggagatggt gaagaccatg gcccccaagg ccgtcatctt
     2161 cgccatggcc aacccgaccc cggaaattta ccccgacgag gccatcgccg ccggagcagc
     2221 agtggtggcc accgggagaa gcgattttcc caaccagatc aacaactgtc tcggcttccc
     2281 cggcattttc cgcggagccc ttgatgtgcg ggcctcggtc atcaacgagg agatga
//
LOCUS       DAOTHD010000308         2336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_54285_length_2336_cov_2.073214, whole genome shotgun sequence.
ACCESSION   DAOTHD010000308 DAOTHD010000000
VERSION     DAOTHD010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54285_length_2336_cov_2.073214"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="P5219_06085"
     CDS             complement(<1..900)
                     /locus_tag="P5219_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRX26359.1"
                     /translation="MKTVTVGIIGAGFASKFHCRSFSRVAGINVRVKSIADVDAGKAS
                     AVAAEFGLEEFHNDCTALLDDPEIDVIDICTPPFLHPGMTMEALAAGKHVICEKPLTG
                     YFGAPGDETPIGDRVPKSVMYDKVLGDIRNLGKVVAESNRLFMYAENYVYSPNILKAA
                     EIIRARKSRILFMKGEESLKGSSSPVAGRWDKTGGGSLIRVGCHPIGGILWLKREEAR
                     TRGISIEIESVLCDTARMTPSLSDHERRHLSVRPEDVEDFASLVLTFTDGTGAVVIAG
                     DTMLGGTRNYVEVYANDAVMHCMI"
     gene            complement(988..1629)
                     /locus_tag="P5219_06090"
     CDS             complement(988..1629)
                     /locus_tag="P5219_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HRX26360.1"
                     /translation="MTLLKNRSLEDFSGELAASSAAPGGGSAAALSGALGAALVSMVC
                     RLTLGKKEYEEYEEELREVLVLSEDLRKNLVNAVDVDAAAYGFVMEAFALPKETEEEK
                     AVRREAIQKAFREACESPRKTSFWCLEVLRLCARVSGKVNVNAASDLGVGANQALAGL
                     EGAAMNVLINLPSIKDDEYTDLLREEVEEIQDEGRILKTGVMREVLAYIGGAD"
     gene            complement(1799..>2336)
                     /locus_tag="P5219_06095"
     CDS             complement(1799..>2336)
                     /locus_tag="P5219_06095"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843924.1"
                     /note="catalyzes the formation of
                     5,10-methenyltetrahydrofolate from
                     5,10-methylenetetrahydrofolate and subsequent formation of
                     10-formyltetrahydrofolate from
                     5,10-methenyltetrahydrofolate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HRX26361.1"
                     /translation="QIDPDTVLAAIRPEKDVDGFHPVNVGRLVAGLPACEPCTPKGIL
                     RLLKSTEIPLAGKEAVVIGRSNIVGKPVALMLLAESATVTVCHSRTRDLAEHVRRADI
                     LVAAIGKPRFVTADMVKEGAVVVDVGINRLEEGLVGDVDYGPVSEKASWITPVPGGVG
                     PMTIAMLLENTLQQAKKV"
BASE COUNT          401 a          758 c          659 g          518 t
ORIGIN      
        1 gatcatgcag tgcatcactg cgtcattggc gtagacctcc acgtagtttc tcgtgccgcc
       61 cagcatggtg tcgcctgcaa tcaccaccgc cccggttccg tcggtgaagg tgaggaccag
      121 ggaggcgaaa tcctccacgt cctccgggcg gacgctgaga tggcgccgtt cgtgatcgct
      181 cagggagggc gtcatacggg ccgtgtcgca caggacactt tcaatttcta tggaaatccc
      241 tctggtccgt gcctcttccc tcttcagcca gagaatcccc ccgatggggt ggcaccccac
      301 ccggatgagg gaaccgccgc ccgttttgtc ccacctgccg gccaccggtg acgaggaccc
      361 tttcaggctc tcctccccct tcatgaaaag gatcctgctc ttccgggccc ggatgatctc
      421 tgcggctttc agaatattcg gcgagtagac gtaattctcg gcatacatga agagcctgtt
      481 gctttccgct acgaccttcc cgaggttccg tatatccccg agcaccttgt cgtacatgac
      541 cgatttgggc accctgtctc cgatgggcgt ctcgtcgccg ggagcgccga aatagccggt
      601 caggggcttt tcgcatatca cgtgcttccc tgccgcgagg gcttccattg tcatgcccgg
      661 gtgcaggaag ggaggggtgc agatgtcgat gacgtcgatc tccggatcgt caaggagggc
      721 ggtgcagtca ttgtggaatt cctccaggcc gaattccgcc gcgacggcgg aagcctttcc
      781 ggcatctaca tccgcgatgg atttcacgcg gacgtttatt cccgccaccc gggaaaagga
      841 ccggcagtgg aacttcgacg caaagccggc accgatgatg cctaccgtga ccgtcttcat
      901 tttttgcctc tttttctgtt cgccggtttc cgatggagag ggaaactgcg tcccgaaaat
      961 ggtttcatga aaggagaacc gcccggctca gtccgctccg ccgatatagg ccaggacttc
     1021 cctcatcacc ccggtcttga gaattctccc ctcgtcctgg atctcctcga cttcctcccg
     1081 aagaagatcg gtgtactcgt cgtccttgat ggagggaagg ttgatcagca cgttcatggc
     1141 agccccttca agccctgcca gggcctggtt ggctcccacg ccgaggtcgc tggccgcgtt
     1201 gacgttcacc ttgcccgaca cgcgggcgca gaggcgaagc acctcgaggc accagaagga
     1261 tgtttttctc ggcgattcgc aggcttcccg gaacgccttc tgtatggcct ctctccggac
     1321 cgccttttct tcttcggtct ccttcggaag ggcgaaggcc tccatcacaa atccataggc
     1381 cgcggcatcc acgtcaacgg cgtttaccag gttctttctc aggtcctcgg agaggacgag
     1441 aacctccctg agctcctctt catactcctc gtattccttt ttgccgagag tgagccggca
     1501 caccatggaa acaagggccg caccgagggc tcccgaaagg gcggcggcgc tgcctcctcc
     1561 cggcgccgcc gaactggcag caagctctcc cgaaaaatct tcaaggctcc tgtttttcag
     1621 aagggtcatg gacgtctctt cctccttgtg gaattttcct gcgtccgggg catttccccc
     1681 gtacgggcca ttcaggtatc tttataccac agagccgctc ctttgataaa cgccgtcccc
     1741 ccggtattct ccgggaggac ggccgaagac ggtgctgttt gtcccttggg ggtcgttttc
     1801 agactttctt tgcctgttgc agggtattct ccagcagcat ggcgatggtc atgggcccca
     1861 cccctcccgg aacgggagtg atccacgacg ccttctccga gacaggaccg taatccacgt
     1921 ccccgacgag gccttcctcg agcctgttga ttcccacgtc caccaccacg gcgccttcct
     1981 tcaccatgtc cgccgtgacg aaacggggct tcccgatggc tgcaacaagg atatccgccc
     2041 tgcggacatg ctccgcgaga tcccttgtcc tgctgtggca gacggtcacc gtagcgctct
     2101 cagccaggag catgagggcc acaggcttgc ccacgatgtt gcttctcccg atgaccacgg
     2161 cttcctttcc cgccaggggg atctccgtgc ttttgagaag gcggagtatt cccttggggg
     2221 tgcagggttc gcaggccggg agccctgcca cgagacgccc gacgttcacg gggtggaagc
     2281 cgtccacgtc cttttccggc cggatggccg cgaggacggt gtcgggatcg atctgc
//
LOCUS       DAOTHD010000309         2335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_54306_length_2335_cov_3.125000, whole genome shotgun sequence.
ACCESSION   DAOTHD010000309 DAOTHD010000000
VERSION     DAOTHD010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54306_length_2335_cov_3.125000"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..771)
                     /locus_tag="P5219_06100"
     CDS             complement(205..771)
                     /locus_tag="P5219_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRX26362.1"
                     /translation="MKRFDIYMSGVGGQGIGLLSEAVIRAADHAGLSVCGVDTHGLAQ
                     RGGMVESFVRIGGEVTTPLVRKGSAFLVLALERTEALRAAEAYAASGGVLLYFDTLWQ
                     PLDVRLGKADAVSAEDVEEACRRLNVRHMAEKRDLPDVRMQNVALLSRAAKEGLIPGV
                     EMEHYRKALEDLLPGKVLAANLKVLEGQ"
     gene            complement(768..>2335)
                     /locus_tag="P5219_06105"
     CDS             complement(768..>2335)
                     /locus_tag="P5219_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HRX26363.1"
                     /translation="ELEGGLVAVLGDDPGANSSQNEQDNRHFARMAYIPVLEPSSPSE
                     AYRYYLEAMALSRRQKMPVILRMTTHVCHGKEKVAFGAWKGGTVSTESRFDTSGGPMV
                     PLTAAALEMKRRSLARLSLAGCEMDSPAFCEVRKGTDSSRGVITAGLPFRSLLDVLED
                     SDRTPDILKLGAVYPLPEKAVEEFLRTHGQVKVLEELDDVLEKDIKALAFDRGIRVSI
                     TGKTDDDDRIGEFTPDKVAEVLAKTWPDMVPLRGAKRQPSLAPRPPQMCPGCGHRSAF
                     HAIRQALAKEDITVADIGCHTLGFLPPYEMGEVLLSMGHSTGTGAGLSLFNRSRKVVS
                     FIGDSTFFHAGLPGVVNAVFNNHNLTLIVMENGTTAMTGHQDHPGAGRNANGPSEAIP
                     VRGVLEGLGVKSIREVDAYSQAKLIELVKEAMAEEGFSVVIARHPCMLKYTREQQRNP
                     DYVRKSVDVNQETCDRLHVCVESFGCPSFQRDEDGTVTVSRELCIGDGSCIQTCPVKA
                     IGLRKENRGGETA"
BASE COUNT          382 a          801 c          709 g          443 t
ORIGIN      
        1 accggaggaa gcccctgccg ctccgccacc atgttcatgg tgtccctgat ggcgtagctg
       61 ctgtccacga gggtcatgtc gaagtcaaaa atcacgcatc gaaacactcc ataccacctc
      121 tttctcactt ctctttaatg gtaccctgcg gccggaaaaa agaaaaggac cgcccgggag
      181 gacggccccc tgaaacttct gaaatcactg tccctccagc accttcaggt tagccgccag
      241 gacctttcca gggagcaggt cctccagggc cttccggtag tgctccatct ccacgccggg
      301 gatgagcccc tcctttgccg ccctcgacag cagggccacg ttctgcatcc tcacgtcggg
      361 aaggtccctc ttttccgcca tgtgccggac gttcagccgc ctgcaggcct cctccacgtc
      421 ttcggcggag acggcgtccg ctttcccgag ccggacgtcc aggggctgcc agagagtgtc
      481 gaaatagagc agaactcctc cggaggctgc ataggcctcc gccgcgcgca gggcttcagt
      541 ccgctccagg gcgaggacga ggaatgcgga gccctttctc accagcgggg tggtcacctc
      601 gccgccgatc ctgacgaagg actccaccat gccgcccctc tgggcaaggc cgtgggtgtc
      661 cacgccgcag acggaaaggc ccgcatgatc ggcggcacga atgaccgcct cgctcaggag
      721 accgattccc tggcctccca cgccggacat gtagatatcg aaccgcttca tgctgtctcc
      781 cctcccctgt tctccttccg aagtccgatg gctttcaccg ggcaggtctg gatgcaggat
      841 ccgtcgccga tgcacagttc gcggcttacg gtcaccgttc cgtcctcatc cctctggaag
      901 gacgggcacc cgaaggactc cacgcacacg tggagccggt cgcacgtctc ctggttcaca
      961 tccaccgact tccgcacgta gtccgggttc cgctgctgct cccgggtgta tttcagcatg
     1021 caggggtgcc gggcgatcac cacggaaaag ccctcctcgg ccatggcttc cttcaccagt
     1081 tcgatgagct tcgcctggct gtaggcgtcc acctcccgga tgctcttcac cccgagcccc
     1141 tcgaggaccc cccggacggg aatggcctct gacgggccgt tggcgttccg ccccgcgccg
     1201 gggtggtcct ggtggcccgt catggcggtg gtgccgtttt ccatgacgat gagggtcagg
     1261 ttgtggttgt tgaacacggc gttcaccacc cccggaaggc ccgcgtggaa gaaggtggag
     1321 tcaccgatga agctgaccac cttccgcgac ctgttgaaca gggagagccc cgcccccgtc
     1381 ccggtggagt gccccatgga gaggagcacc tcccccattt cgtagggggg caggaagccg
     1441 agggtatggc atccgatgtc cgccacggtg atgtcctctt tggccagggc ctgccggatg
     1501 gcatggaacg ccgaccggtg gccgcacccc gggcacatct ggggcggcct gggggccagg
     1561 gacggctgcc tcttggcccc cctgagggga accatgtcgg gccatgtctt ggccagcact
     1621 tcagccacct tgtcgggggt gaactccccg atccggtcgt cgtcgtcagt ctttcctgtg
     1681 atggaaaccc ttatgcccct gtcgaaggcc agtgccttga tgtccttttc cagcacgtcg
     1741 tccagttcct cgaggacttt cacctgtccg tgggtgcgga gaaactcttc cacggccttt
     1801 tccggcaggg ggtacacggc tcccagcttc aggatgtcag gggtccggtc cgaatcctcc
     1861 agcacgtcca ggagggaccg gaacggcagc cccgcggtga tgacgcctct cgaggaatcc
     1921 gtcccctttc tgacctcgca gaacgcgggg gaatccatct cgcatcccgc aagggaaagc
     1981 cgggccaggg acctgcgctt catctccagc gcggctgccg tcaggggcac catggggccg
     2041 cccgaggtgt cgaaacggga ttcagtactg acggtaccgc ccttccacgc cccgaaggcc
     2101 accttttctt ttccgtggca tacgtgggtg gtcatccgga ggatcacggg catcttctgc
     2161 cgccgggaca gggccatggc ctcgaggtag taccggtagg cctccgacgg cgaggacggc
     2221 tccagtaccg ggatgtaggc catccgcgcg aagtgccggt tgtcctgctc gttctgggac
     2281 gagtttgccc cggggtcgtc gcccaggacg gccacgagcc ctccctccag ttcca
//
LOCUS       DAOTHD010000310         2334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_54336_length_2334_cov_2.957876, whole genome shotgun sequence.
ACCESSION   DAOTHD010000310 DAOTHD010000000
VERSION     DAOTHD010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54336_length_2334_cov_2.957876"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="P5219_06110"
     CDS             complement(<1..620)
                     /locus_tag="P5219_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRX26364.1"
                     /translation="MKKENSQYLSMTQMPVGRLVVSLAVPTIISMLVTAVYNIGDTYF
                     VSKLGTSASGAVGIVFSLMAIIQAVGFTIGMGSGAQISRLLGAQKPEEADRVAASGLL
                     AAVSFGTLLAVFGLLFRDGLMRLLGATETILPYARDYAGYILYAAPVMASSFLLNNIL
                     RAEGKARFAMVGLTTGGILNLLLDPLFIFGFGMGIAGAAIATAISQ"
     gene            complement(680..2290)
                     /locus_tag="P5219_06115"
     CDS             complement(680..2290)
                     /locus_tag="P5219_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HRX26365.1"
                     /translation="MTWGSAFQILGGVGLFLFGIKLMSEALQYLAGDKMRQLIGSLTK
                     TPIRGVFIGALVAMLIQSSSGTTVMTVSFVHAGLMTLKQAVGVIMGANIGTTVTAQIV
                     AFKIKDFALPILGLGVLLVLFGRTKRQKYIGNGMVGFGLLFLGMQTMESSMVFLRDRK
                     DLFLAFSSNPLLGVLVGTAVTMVVQASSATIGLTMAMAAQGLLTLDAAIPILLGDNIG
                     TTITAVIASIGSNRSAKQAATAHVLFNVIGATIFLAAFPLFKDVIVSTSGDISRQLAN
                     AHTIFNVTNTVMFLPFVSLFVWLIRRIVPVRGEAMAAGPIYLDLKLIGASPAAAVDAV
                     KKEILHMGTLAASMLRDVRRAFEENDPKMINEVNQTEKGVNEINRAITSYASEIWQKG
                     LSSDLSTVLGSYVNGVGDIERIGDHATNLIELYEYKIDHGVEFSSLAMEEFRDMFNAV
                     QEAVRLSFESLDEENVEKAREVDSLEDEVDRKEKTLRKNHITRLNRGECTPQGGVIFI
                     DILSNLERVCDHAHNLSFIAIDISRLHR"
BASE COUNT          467 a          683 c          698 g          486 t
ORIGIN      
        1 cactggctga tggccgtggc gatggccgcc ccggcgatac ccatcccgaa accgaaaata
       61 aagaggggat ccagcagcag gttgagaatg cctcccgtcg tcagccccac catggcgaac
      121 ctcgccttcc cttcggccct gaggatgttg ttcagcagga aggacgacgc catgacgggc
      181 gcagcataca ggatatagcc ggcataatcc cgggcgtagg gcagtatggt ctccgtggcg
      241 ccgaggaggc gcatcaggcc gtcacgaaac agcaggccga agactgcgag gagggttccg
      301 aaggagaccg ccgcgagaag tccgcttgcc gcgaccctgt cggcctcttc gggtttctgt
      361 gctcccagga gccgggagat ctgcgctccg gaacccatgc cgatggtgaa ccccaccgcc
      421 tggataattg ccatcaggga aaaaacgata ccaacagcgc ccgaggcgct tgtgccgagc
      481 ttcgagacga aataggtatc gccgatattg tacacagccg tcaccagcat gctgatgatc
      541 gtcggcaccg caagggaaac gacgagcctg cccacgggca tctgcgtcat gctgagatat
      601 tgggagtttt cttttttcat cgtttcaaac tcatttttac ggcggttctt ccgttttcat
      661 cctttgggac actcttccct cagcggtgga gcctggaaat gtctatggcg atgaaggaaa
      721 ggttgtgggc atggtcacag actctctcca ggttgctgag gatgtcgatg aagatgactc
      781 ctccctgggg agtgcattct ccccgattga gcctggtgat gtggttcttt ctcagtgtct
      841 tctccttgcg gtccacctcg tcttcgaggc tgtccacttc cctggccttc tccacgttct
      901 cctcgtcgag agattcgaaa ctcagccgca ctgcctcctg cacggcgttg aacatgtccc
      961 tgaattcttc catggcgagg gaggaaaact cgaccccgtg gtcgatcttg tattcataga
     1021 gttcgatgag gttcgtggca tggtctccta tccgttcgat gtcccccaca ccgttgacat
     1081 acgagcccag caccgtggac aggtcgctgg aaagtccctt ctgccatatt tccgaggcat
     1141 aggaggtgat ggcgcggttg atttcgttca cgcccttctc cgtctggttt acctcgttga
     1201 tcatcttcgg gtcgttttcc tcgaaggccc ggcgcacatc ccgcagcatg gacgcagcaa
     1261 gggttcccat gtgaaggatc tctttcttca cggcatccac ggctgccgcc ggggatgcgc
     1321 cgataagctt caggtcgagg tatatcggcc cggccgccat tgcctccccc ctcaccggga
     1381 cgatgcgtcg gatgagccag acgaagaggg acacgaaggg aagaaacatc accgtgttgg
     1441 tcacgttgaa gatggtgtgg gcgttggcga gctgcctgct gatgtcgccc gaggtggaca
     1501 cgatgacgtc cttgaacagg ggaaaggccg caagaaaaat ggtcgccccg atgacgttga
     1561 agagcacgtg ggccgtggcc gcctgtttcg ccgacctgtt ggagccgatg gaggcgatga
     1621 ccgccgttat ggtggtgccg atgttgtcac cgaggaggat cggtatggca gcgtccaggg
     1681 tcaggagccc ctgggccgcc atggccatgg tgagaccgat ggtggcggag ctggcctgga
     1741 ccaccatggt gaccgccgtt ccgacgagga ccccgagcag ggggttggag ctgaaggcaa
     1801 ggaagaggtc cttcctgtcc cgcaggaaaa ccatggacga ttccatggtc tgcattccaa
     1861 ggaacaggag gccgaagccc accattccgt tgccgatgta cttctgccgc ttggtcctcc
     1921 cgaagagaac aaggagaact cccagcccca gtatcggaag ggcaaagtcc ttgatcttga
     1981 aggcaacgat ctgggcggtc accgtggtgc cgatgttggc ccccatgatg acccccacag
     2041 cctgcttcag ggtcatgagc cccgcgtgaa cgaagctcac cgtcatgacc gtggtgccgc
     2101 tgctgctctg gatgagcatg gccaccaggg ctccgatgaa cacccccctg atgggggtct
     2161 tggtcaggga gccgataagc tggcgcatct tgtcgccggc gagatactga agggcctcgc
     2221 tcatgagctt gatgccgaaa aggaaaagac ctacgccgcc gagaatctga aacgcactgc
     2281 cccacgtcac aacaacatcc cctgcccctt cttcgttccg ttttctgtat gaag
//
LOCUS       DAOTHD010000311         2317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_54891_length_2317_cov_2.407604, whole genome shotgun sequence.
ACCESSION   DAOTHD010000311 DAOTHD010000000
VERSION     DAOTHD010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54891_length_2317_cov_2.407604"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    165..264
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(532..>1356)
                     /locus_tag="P5219_06120"
     CDS             complement(532..>1356)
                     /locus_tag="P5219_06120"
                     /inference="COORDINATES: protein motif:HMM:NF037272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin like domain-containing protein"
                     /protein_id="HRX26366.1"
                     /translation="FQSGGHAVAIAGWDDDYPAANFVRDPGRNGAWLVKNSWGEAWGN
                     DGYFWVSYADPTLNSPSADIGGEPDIFDSVYQYDPLGWVTGYGTGNETTWFANVFAAS
                     EGRPGLAEVLQAVSFYSAASESSYRIEIRRNVSRAAPFENAPVSVKEGVLGPSGYHTV
                     RLDREVFLSPDSLFSVAVKITTPGYAFPVPLESFESGYSDKVEAEPGQSFISADGVSW
                     SDLTALPGHEKSNVCLKAFTSFEETSGGGCSSRGEIGFAGLLLLLPLVPVLWRRGK"
     assembly_gap    1357..1366
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1367..2216)
                     /locus_tag="P5219_06125"
     CDS             complement(<1367..2216)
                     /locus_tag="P5219_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HRX26367.1"
                     /translation="MRLTRSVLFAAACIFLLFPVLSASGSWRTAPLNPEFLAQRTGES
                     FNLHSMGGPGRRNTAQRRSLGEEPSPLDLSHVRAPVHFSGIRGGGDVSAAAVSFPSSF
                     SLPTEGRMTPIRDQAPYGTCWAFGAFASLESFFAGQGEENLDFSEWHLAYFAYVDEGE
                     GLPGFTGNTDPHFGGDPIFDQGGNAWMSAALLARWTGAVTEADRPYQNVTPWPEESRP
                     LSSDRAAKRLEHVLYLGSAPDMDSIKYALTHYGAVRIGVRWPWWDFEDQVLSPSFAFY
                     NYDPVGF"
BASE COUNT          493 a          661 c          662 g          391 t
ORIGIN      
        1 cgagatcctt cggccaaaaa accggcctca ggatgacagt cttgtttgtc atcccaagcg
       61 caacacccaa gcgaagcgga gggaggagca tccccggagg ggaaggatct cgcctcggct
      121 tgtcatcccg aggccggcat catagccgag ggatctcggg tttgnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnacggca ctcagaacca aaggcgagat ccctccgccc
      301 ttcggtcgtt cgggatgact aacaaatgcg gtcgcgcagc atccttgaga gagagcggaa
      361 tcaaaatcga ggtccttcgc gttgctcagg acgacagaaa ctactgtcat cccgagcgaa
      421 tgcgagggat ctcgggtttg acggcactca gaaccaaagg cgagatccct ccgcccttcg
      481 gtcgttcggg atgacaaaac ccatccttcg cattcgctcg ggatctcggt tttatttccc
      541 ccgcctccag agcaccggaa ccagaggcag aaggagtaaa agccccgcga atccgatttc
      601 cccccgtgaa gaacagccgc ccccggaagt ttcctcgaag gaagtgaagg ccttcaggca
      661 gacattggac ttttcgtggc cgggaagagc cgtcaaatcg ctccaggaaa ccccgtcggc
      721 gctgatgaag ctctgccccg gctccgcctc gaccttgtcg ctgtagccgg attcaaatga
      781 ttcgagagga acgggaaaag cgtagccagg cgtggtgatc ttcaccgcca cggaaaagag
      841 actgtccggg gagagaaaca cctctctgtc gagccgaacc gtatggtacc ccgaaggtcc
      901 aagcacccct tccttcactg aaaccggggc gttttcaaaa ggagccgcac gggacacatt
      961 ccgcctgatc tcaatgcgat acgaactctc cgaggcggcc gaatagaagg aaacggcctg
     1021 aaggacttcg gccagcccgg ggcgcccttc cgaagcggca aagacgttgg cgaaccaggt
     1081 ggtctcgttc ccggtgccgt aaccggtcac ccatccaagc gggtcgtact ggtagacgga
     1141 atcgaagatg tccggttctc caccgatgtc cgccgagggg ctgttcaggg tggggtcggc
     1201 gtaggaaacc cagaaatacc cgtcatttcc ccatgcttcc ccccagctgt tcttcaccag
     1261 ccaggcgccg ttccgtccag gatccctgac gaaattggcc gcggggtaat cgtcgtccca
     1321 accggcaatg gccaccgcat ggcctccgct ctgaaannnn nnnnnngaaa gcccaccgga
     1381 tcgtagttgt aaaaggcgaa ggaagggctg agcacctggt cctcaaaatc ccaccagggc
     1441 catctgacgc cgatgcggac ggctccgtag tgcgtgaggg cgtattttat gctgtccata
     1501 tcgggcgccg agccgaggta gaggacatgc tccagcctct tcgccgccct gtccgaagag
     1561 aggggcctgc tttcctccgg ccagggggtg acgttctggt agggccggtc agcttccgtc
     1621 accgcgccgg tccagcgggc aagaagtgcc gcggacatcc atgcgtttcc cccctggtcg
     1681 aaaatggggt ctccgccgaa atgggggtcc gtgtttcctg tgaatcccgg cagtccctcc
     1741 ccttcgtcca cataggcgaa ataggcgaga tgccactccg aaaaatcaag gttctcctcc
     1801 ccctggccgg caaaaaagga ctcaagggaa gcaaaagcgc cgaaagccca gcaggttccg
     1861 tagggcgcct ggtcccgtat aggggtcatc cgtccttcag tggggaggct gaaggatgaa
     1921 gggaaagaaa cagcggcggc agacacatcg cccccgcccc ggataccgga gaaatgcacg
     1981 ggagccctca catgggaaag atcgagggga gaaggttcct ctccgagcga ccggcgctgt
     2041 gcagtgttcc tcctgcccgg tccacccata gaatggagat tgaagctctc tcctgtccgt
     2101 tgggcgagaa actcggggtt gagaggggct gttctccacg aaccggaagc ggacaggacc
     2161 ggaaaaagca gaaaaatgca cgcggcagcg aaaagaacgg aacgggtcag tctcatggaa
     2221 gggtctctcc tttttttcca aaagttgcgg tatcggtcaa gctgtcatcc cgggcgaatg
     2281 cggtctgtca tcccgagcgg acgcgaggga tctcggg
//
LOCUS       DAOTHD010000312         2314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_54983_length_2314_cov_2.852590, whole genome shotgun sequence.
ACCESSION   DAOTHD010000312 DAOTHD010000000
VERSION     DAOTHD010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54983_length_2314_cov_2.852590"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..495)
                     /locus_tag="P5219_06130"
     CDS             complement(145..495)
                     /locus_tag="P5219_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26368.1"
                     /translation="MRHERLVVSRIVGELMNFFFSMGARDFQARVARSDEGHEIVIES
                     DYAGNQGSKLREMTRLLRMPRAREMEQYSWSLSGDISTGQEIYLVGILTDTVSVDHDE
                     QAGKVRIVLFRKWN"
     gene            complement(492..1769)
                     /locus_tag="P5219_06135"
     CDS             complement(492..1769)
                     /locus_tag="P5219_06135"
                     /inference="COORDINATES: protein motif:HMM:NF019244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HRX26369.1"
                     /translation="MKASGKIGFEKALVGERGKAAFLFSFGIALCIFGLTRDSLQGIL
                     SGLWRILVEPDYLISDYMDVGGMGAAFINSGLLTVIFTSILVFLRVHIRGISIAAVFT
                     VAGFSFFGKNLLNVWFIMAGVWLYARSRKEPFLNYVYVAFFSTALAPLVSQLMFGFHF
                     PPYLRIVLGAAAGVSAGFLLPPLATSFLSVHHGFNLYNMGFTAGMVGTLYVSVFRSHG
                     FITASRVIWSTGHNGLLWPLCAAFFLFLAAAGLLLGGLSQGGLRELWKHPGRLIADFV
                     DLYDFPATLMNMGLTGLAATAYLRLSGGDFNGPTLGGLLTIAGFSAMGKTPRNIVPIL
                     LGVVLGSITKDWSLTDPAIQLAALFGTTLAPIAGEFGAAAGVLAGYIHSSVVLNVGVL
                     HAGFNLYNNGFAGGLVAAILVPLIEAFRRREKR"
     gene            complement(1896..>2314)
                     /locus_tag="P5219_06140"
     CDS             complement(1896..>2314)
                     /locus_tag="P5219_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26370.1"
                     /translation="AGSFLLIMSVPEPLIRIFNDTDPELLALSVRGMRIGYSGVMFAS
                     VSIIASHTFQAIGKPKKGLFLTLSRHFLFILPPLYFLPPLLGTDGVWMALPLSDLGGG
                     LLGAWFLKREFAVMRRNQRIDGMLPLPECTPPPDSV"
BASE COUNT          516 a          705 c          681 g          412 t
ORIGIN      
        1 cgatcttcag ggccatttct cccacttcaa gagcttcctc caccgttcct cctcctcctc
       61 cgccgagcag cgaggcgccg aggacggcgg cttccagcag ttcccgggtg agttccgtct
      121 gtgccatgat tttctccctt ccgttcagtt ccacttccgg aacaggacga tacgtacctt
      181 tcccgcctgt tcgtcgtggt ccaccgagac ggtatcagtc aggatgccca cgaggtagat
      241 ctcctggccc gtactgatgt cccccgacag cgaccatgaa tattgctcca tctcccgggc
      301 ccggggcatc cgcagcagcc gggtcatctc ccggagcttg ctcccctggt tcccggcgta
      361 atccgactcg atgacgatct cgtgcccctc gtcgctcctg gcgacccggg cctggaaatc
      421 cctggcgccc atggagaaaa aaaagttcat cagttcgccc acgatgcggg acaccacgag
      481 acgctcgtgc ctcatcgttt ctccctcctc cgaaaagcct cgatgagggg aacaaggatg
      541 gcggccacca gtccccccgc aaagccgttg ttgtacaggt tgaaacctgc gtggagcact
      601 cccacgttca gcaccacaga cgagtggatg tagcccgcca ggacaccggc ggccgccccg
      661 aattccccgg cgatgggggc cagggtggtc ccgaaaaggg cggcaagctg gatagccgga
      721 tctgtcaggc tccagtcttt cgttatgctg cccaacacaa cgccgagcag gatgggaacg
      781 atattgcggg gtgttttccc catggcgctg aaaccggcga tggtaagaag cccgcccagg
      841 gtcggcccgt tgaagtcgcc cccgctcagc cggagatagg ctgtggccgc aaggcccgtc
      901 agccccatgt tcatgagcgt ggcgggaaaa tcatacaggt cgacgaaatc agctatgagg
      961 cgtccgggat gcttccagag ctctctcagg ccgccctgcg aaaggccgcc gagaagcagt
     1021 ccggcagcag cgagaaaaag aaaaaaggcg gcgcacagag gccacagaag gccgttgtgt
     1081 cccgtagacc agatgacgcg gctcgccgtg atgaagccgt gggaacggaa gacggacaca
     1141 tacagggttc ccaccattcc ggcggtgaat cccatattgt acaggttgaa tccgtggtgg
     1201 accgacagga aggaggtggc gagaggggga agcagaaagc cggccgaaac ccctgccgct
     1261 gcgccgagaa caatcctcag gtagggagga aagtgaaagc cgaacatgag ctggctcaca
     1321 aggggcgcaa gggcggtgct gaaaaaggcg acgtacacgt aattgagaaa gggttctttc
     1381 cggcttctcg cgtagagcca gactccggcc atgataaacc agacgttcag cagattcttc
     1441 ccgaaaaagg agaaccccgc cacggtaaaa accgctgcga tggaaatccc cctgatgtgg
     1501 accctgagaa atacaaggat ggacgtgaag atcaccgtga gcagcccgct gttgatgaag
     1561 gccgccccca tgccccccac gtccatgtag tccgaaatga ggtagtccgg ttcgacaaga
     1621 atcctccaca ggcccgacag gataccctgc agggaatccc ttgtgaggcc gaaaatgcag
     1681 agggctattc cgaaggaaaa aagaaacgcc gcttttcctc tttcaccgac gagagccttt
     1741 tcgaatccga tttttcccga ggctttcatg cagaaaacct tttctccggg atcatttcgc
     1801 cggccggttt cactccgccc ctgcctatgg ggcctgctct tctctccatg tatttcacct
     1861 ccgggatttc cagggggaca gcgtctgttc ctccattata cagaatccgg agggggcgta
     1921 cattccggga ggggcaacat cccgtcgatc cgctggtttc gccgcatgac ggcgaactcc
     1981 cgcttcagga accaggctcc caggagccct cctcccaggt ccgacagggg aagagccatc
     2041 cagacgccgt ccgtccccag gagaggggga aggaagtaga gcggcgggag aatgaagaga
     2101 aagtgccggg aaagggtgag aaacagccct tttttgggct tcccgatggc ctgaaaggta
     2161 tgggaggcaa taatgctcac cgaggcgaac atgaccccgc tgtagccgat ccgcatcccc
     2221 cgcacggaaa gggccagcag ctcgggatcg gtgtcgttga agatgcggat cagcggttca
     2281 ggcaccgaca tgatgagcag aaagctcccc gcga
//
LOCUS       DAOTHD010000313         2314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_54984_length_2314_cov_2.822045, whole genome shotgun sequence.
ACCESSION   DAOTHD010000313 DAOTHD010000000
VERSION     DAOTHD010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54984_length_2314_cov_2.822045"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /gene="larE"
                     /locus_tag="P5219_06145"
     CDS             <1..712
                     /gene="larE"
                     /locus_tag="P5219_06145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HRX26371.1"
                     /translation="FAARLGIRHVVLPVSELDCPDFVRNDGERCYHCKKYRFELLCRW
                     AEEQGIFWVIEGSNTDDLQDYRPGMRAVAELERVRSPLLEAGFSKAAIRELSREWGLP
                     SWEKPAAACLASRIAYGTPITGENLRQVEQAEDIVRSFCPPNAQIRVRHHGAIARIEA
                     EPEVLFLLAEPARAAGIAASLAALGFDWVTLDLAGYRMGSMNELLADRRNRSGGDPLA
                     VEVDFKAAGPDVEETQGG"
     gene            complement(611..1180)
                     /locus_tag="P5219_06150"
     CDS             complement(611..1180)
                     /locus_tag="P5219_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRX26372.1"
                     /translation="MDTLLQGYVRFRERADRNAYCALCSGQAPHTLVITCSDSRIVPE
                     DIFDARPGELFVLRNVGNLARTDDPSVAAALDYALLHLGVKNVVVLAHTDCGAVKASR
                     EPEHLDTEGLRLWLGEEHFDGSDVEQASRAAGVRQLDRIRKYPLFRKLEGEGKTSASL
                     CFFDIGTCGLEIYLNGQWVAPGTVSAICK"
     gene            complement(1220..1690)
                     /gene="msrB"
                     /locus_tag="P5219_06155"
     CDS             complement(1220..1690)
                     /gene="msrB"
                     /locus_tag="P5219_06155"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRX26373.1"
                     /translation="MTGLLLLFAGAFLLVPARDSLAAVPDRVEKSEEEWKALLTPEQF
                     HVARQKGTEPPFTGKYWDFHEEGTYACVCCGAELFSSRDKFDSGSGWPSFTAVLEHAP
                     IAEKRDFSYGMVRTEVLCARCDAHLGHLFSDGPPPTGMRYCINSASLLFLPETR"
     gene            complement(1701..1976)
                     /locus_tag="P5219_06160"
     CDS             complement(1701..1976)
                     /locus_tag="P5219_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26374.1"
                     /translation="MEHVSAIITRFIRQNMEERGLVLYFTDDDKLLAMDDRFETHFKF
                     DLVFSDNDFSCQVLARGEKGLQVRQRFNISWTNAKGIREFMDYVRSL"
BASE COUNT          487 a          686 c          683 g          458 t
ORIGIN      
        1 gttcgccgcc cgtctcggca tacggcacgt ggttctgccc gtctcggaac tggattgccc
       61 ggattttgtc cggaacgacg gcgaacgctg ctaccactgc aaaaaatacc gtttcgagct
      121 gctttgccgc tgggcggagg agcagggcat tttctgggtt atcgagggat cgaatacgga
      181 cgacctgcag gattaccggc ccgggatgag ggccgtggcg gagctcgaga gggtgagaag
      241 ccctctgctc gaggccggtt tttccaaggc ggccatacgg gagctttcca gggaatgggg
      301 gcttccgtcc tgggagaagc cggcggcggc atgcctcgcg agcaggatcg cctacggtac
      361 ccccatcacc ggggagaatc tgcgccaggt cgaacaggcc gaggacatcg tccggtcctt
      421 ctgccccccc aatgcccaga tcagggtacg ccatcacggc gccatcgcca ggattgaagc
      481 cgagccggag gtccttttcc tgctcgccga accggcgagg gcggcaggga ttgccgcctc
      541 cctcgccgcc ctcggatttg actgggttac cctggatctc gccggctacc gcatgggcag
      601 catgaacgag ctacttgcag atcgccgaaa ccgttccggg ggcgacccac tggccgttga
      661 ggtagatttc aaggccgcag gtcccgatgt cgaagaaaca cagggaggct gaggtctttc
      721 cttccccctc gagttttctg aagagaggat atttcctgat gcggtcgagc tgcctcacac
      781 ccgccgccct ggaggcctgc tccacgtccg atccgtcgaa atgctcctct ccaagccaga
      841 gcctgagtcc ttccgtgtcc aggtgctccg gctcacggct ggccttgacc gctccgcagt
      901 cagtatgggc cagcaccacc acgtttttta cccccaggtg aagaagggcg taatcgaggg
      961 cggccgctac cgaaggatcg tcggtcctgg caaggtttcc cacgttcctg agaacgaaaa
     1021 gctccccggg ccgggcatcg aaaatgtcct ccggaacgat cctgctgtcg gaacatgtga
     1081 tgacgagagt gtgaggagcc tggcccgaac agagggcgca gtaggcgttc ctgtctgccc
     1141 gctcccggaa ccggacgtat ccctgaagaa gcgtgtccat gaagataaca gcaacctttc
     1201 ctgaaaaaaa ttgtattccc taccgtgttt cgggaagaaa caggagggac gccgagttga
     1261 tgcagtaccg catccccgtg ggcggcggcc cgtcgctgaa caggtgcccc aggtgggcgt
     1321 cgcagcgtgc gcacagcact tccgttctga ccatgccgta ggaaaaatcc cttttctccg
     1381 ctatgggggc gtgttccagt acggcggtaa aactgggcca tccggacccg gaatcgaact
     1441 tgtcccggga ggaaaaaagt tccgcaccgc agcagacgca ggcgtaggtg ccctcttcat
     1501 ggaaatccca gtatttcccc gtgaagggag gttcagttcc cttctgcctg gccacatgga
     1561 actgttcggg ggtaaggagg gccttccatt cctcctcgga cttctccacc ctgtcgggga
     1621 cggccgcgag agaatcccgg gccgggacca ggaggaaagc gcccgcaaac agaagcagaa
     1681 gaccggtcat gaaggccttc ctacagactc cggacatagt ccataaattc ccggatgccc
     1741 ttcgcattgg tccaggaaat gttgaatctc tgccggacct gcaggccctt ctctcccctc
     1801 gcgaggacct ggcagctgaa atcgttgtct gaaaaaacga ggtcgaactt gaagtgggtt
     1861 tcgaaccggt cgtccatggc aaggagcttg tcgtcgtcgg taaagtacag cacgagccct
     1921 ctctcttcca tgttctgccg gatgaaacgg gttatgatag cggatacatg ctccattgag
     1981 aaatatcctc cctcccctgg tacgggaaat gttcccctat tttacgccat cgcaggaaaa
     2041 agaccatggg ggcaaaaaga aaaaacggcc gaaaaatgat gctctttcgg tggttcccgc
     2101 cgtatcatgc ccagtgagca ggaacatgac agggggaaaa gccgttcctc ggacttcata
     2161 taccccatta tagtatgtgg tggtataatg cgaaaaaaaa ggaggcggaa aaatgaagcg
     2221 tcaaaactgg cggaaagctc tcatggccct cgctgtgctc tcgctcccgg tgaccatgtt
     2281 ttacctctca cctctcctga ccctgcaggg ggcc
//
LOCUS       DAOTHD010000314         2313 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_55006_length_2313_cov_3.113375, whole genome shotgun sequence.
ACCESSION   DAOTHD010000314 DAOTHD010000000
VERSION     DAOTHD010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55006_length_2313_cov_3.113375"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="P5219_06165"
     CDS             <1..241
                     /locus_tag="P5219_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26375.1"
                     /translation="LNVHTIHAEMEGRAQGPILEQILDRCAERGVTFRTLHDVAFEIS
                     VRNPLIHVCDVVPGELPGRSGTVTVQKCAREEGKE"
     gene            238..1557
                     /locus_tag="P5219_06170"
     CDS             238..1557
                     /locus_tag="P5219_06170"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRX26376.1"
                     /translation="MKLAVVGTGYVGLVTGVCLAKFGNDVVCVDTNAKKIEALSRGTV
                     PFYEPGLEEIIRNNLEAGRLSFSTDLPSALDDALMCFITVGTPSLEDGSADLSAVKSV
                     AETIGRSMKEYKLVVVKSTVPVSTCDFVRQAVAAELGKRGEDIPFDVASNPEFLREGA
                     AVRDFLEPDRVVVGVDCERSADIMKELYSFLPPEKVIFMDIRSSEMTKYAANAMLATR
                     ISFMNEVSAVCERLGADVEKVRMGMGSDARIGYAFLSAGCGFGGSCFPKDVRALKEFA
                     RKEGLEMQILQAVEDVNERQKHVLFGKLLRHFGCLKGKTIGIWGLSFKPKTSDVREAP
                     AQALIAALAAAGAKVQAHDPKAMDETREALGDLQGVLYVNNQYDAVNGADALVLVTEW
                     DIYKQPDFKKIRTLLASPVIFDGRNQYSPAEMKRQGFIYYSIGRGTA"
     gene            1618..>2313
                     /locus_tag="P5219_06175"
     CDS             1618..>2313
                     /locus_tag="P5219_06175"
                     /inference="COORDINATES: protein motif:HMM:NF022655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LmeA family phospholipid-binding protein"
                     /protein_id="HRX26377.1"
                     /translation="MRKILPALLCILLLSTSSPAAPKKEFRGSFTPGTNGEKLLSLLV
                     SLTNPEVLELRMDEEPDDQGNVRNIHFLVKGATVGGFRVEKLALEASFVELNPPSKWD
                     PKAKDPIVVRNVLRSNLEAIILEKDINSALSGYAGNDWNRISVDLKPGIIGARGYYHV
                     KNPGLTLLAEVKTGLEIRNGKELWLKNVELKINHDEQTDAVRQAIRDIQPVVDIRDFP
                     FPVTLAVLSVDDQK"
BASE COUNT          491 a          725 c          701 g          396 t
ORIGIN      
        1 gctcaacgtc catacgatcc acgccgagat ggagggccgg gcccagggcc ccatactcga
       61 gcagatcctc gaccgctgcg cggaaagggg agtgacgttc cgaacgctcc acgacgtcgc
      121 cttcgagatc tccgtccgga accccctcat ccacgtgtgc gacgtggttc ccggagagct
      181 tcccggaagg tccggaacgg tgaccgtcca gaagtgcgcc cgggaggagg gaaaagaatg
      241 aagctcgccg tggtgggtac gggctacgtc ggcctggtga ccggcgtctg cctcgcgaaa
      301 ttcgggaacg acgtggtctg cgtggacaca aacgcaaaaa agatcgaggc tctctccagg
      361 gggaccgttc ccttttacga accgggcctc gaggagatca tccggaacaa cctggaggcg
      421 gggcggctct ccttctccac tgatcttcca tccgccctgg acgacgccct catgtgcttc
      481 atcaccgtgg gcactccctc cctcgaggac gggagcgccg atctctccgc agtcaaatcc
      541 gtggcggaga ccatcggccg ctccatgaag gagtacaagc tcgtggtggt caagtccacg
      601 gtcccggtga gcacctgcga cttcgtccgc caggccgtgg ccgcggagct cgggaagcgg
      661 ggcgaggaca tccccttcga cgtggcgtcg aacccggaat tcctcaggga aggcgccgca
      721 gtgcgggact tcctggaacc ggacagggtg gtggtgggtg tggactgcga acggtccgcc
      781 gacatcatga aggagctcta cagcttcctt cctcctgaaa aggtcatttt catggacatc
      841 cgttcgtcgg aaatgaccaa gtacgccgcc aacgccatgc tcgccaccag gatatccttc
      901 atgaacgagg tctccgccgt gtgcgagcgg ctcggcgccg acgtggagaa ggtcaggatg
      961 ggtatgggat ccgatgccag gatagggtac gccttcctca gcgccgggtg cggcttcgga
     1021 ggatcatgct tcccgaagga cgtccgggcc ctgaaggaat tcgcccggaa ggaagggctg
     1081 gaaatgcaga tccttcaggc cgtggaggac gtcaacgaac ggcagaagca cgtcctcttc
     1141 ggaaagctcc tccggcactt cggctgcctt aagggcaaga ccatcggcat ctgggggctc
     1201 tctttcaagc ccaagacgtc ggacgtccgg gaggcccccg cccaggccct catcgccgcc
     1261 ctggcggccg ccggggcaaa ggtgcaggcc catgacccga aggccatgga cgaaaccagg
     1321 gaagcccttg gagaccttca gggtgttctt tacgtcaaca accagtacga cgcggtcaac
     1381 ggcgccgacg ccctggtcct ggtgacggaa tgggacatct acaagcagcc ggatttcaaa
     1441 aagatacgga ccctcctcgc ttccccggtg atcttcgacg gccggaacca gtactccccc
     1501 gcggagatga agcggcaggg tttcatctac tattccatcg ggcgcggaac ggcctgactc
     1561 ccccttccgc cgacggtgct atacttttga caccacaacc aacaggaggg acttatcgtg
     1621 cgtaaaatac tgcccgcgct gctgtgcatc ctgctgctga gcacttcttc ccccgctgca
     1681 ccgaaaaaag aattccgggg cagcttcacc cccgggacca atggagaaaa gctcctttcc
     1741 cttctcgttt ccctcacgaa ccccgaggtt ctcgaactcc gcatggacga agaaccggac
     1801 gaccagggaa atgtccgcaa catccacttt ctcgtgaagg gagctaccgt cggcggattc
     1861 cgggtggaaa agctggctct cgaagcgtcc ttcgtcgagc tgaatccccc gtcgaagtgg
     1921 gacccgaagg cgaaggaccc catcgtggtc aggaacgtcc tgaggagcaa tctcgaggcg
     1981 atcatcctcg aaaaggacat caactcggcc ctcagcgggt acgccggaaa cgactggaac
     2041 agaatttccg tggacctcaa gcctggaatc atcggcgcca ggggatacta ccacgtgaaa
     2101 aaccccggcc tcactctcct ggcggaggtg aagacggggc tcgagatacg caatggcaag
     2161 gaactctggc tgaaaaacgt ggaactgaag atcaaccacg acgaacagac cgacgccgtc
     2221 aggcaggcca tacgtgacat tcagcccgtg gtggacatca gggacttccc cttcccggtg
     2281 accctggcgg tgctctcggt ggacgaccag aag
//
LOCUS       DAOTHD010000315         2313 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_55012_length_2313_cov_2.592560, whole genome shotgun sequence.
ACCESSION   DAOTHD010000315 DAOTHD010000000
VERSION     DAOTHD010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55012_length_2313_cov_2.592560"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..1210)
                     /locus_tag="P5219_06180"
     CDS             complement(95..1210)
                     /locus_tag="P5219_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HRX26378.1"
                     /translation="MESKRRDHLLTLLFLLLTLALFLLPSWKQAPDSAERVKVRVLST
                     DNSNMRQFGIVRTGDQGLRIRILEGSFRGTETDAVNHLAGRLELDKVFVPGDIAFAVV
                     DADAGRIAKASVLDHYRLDTQLRLLALFCALLLLFGGWTGARALLSFFFTGAMIWKVL
                     LPGYLAGLDPVLISLFTALALTAAIVFLVGGVSRKGMAAFLGSALGILLTCGLSLYFG
                     SEFRIHGAVKPFSETLLYSGFAHLDLTRIFLGGIFLASSGAVMDLAMDVSSAMAEIAE
                     KRPDLSRRQLVLSGFAVGRSVVGTMTTTLLLAYTGSYSSLLLVFVAQGVPLANILNMQ
                     YVSAEILHTLVGSFGLVTVAPFTALAGGWLFAGRDRG"
     gene            complement(1287..>2313)
                     /locus_tag="P5219_06185"
     CDS             complement(1287..>2313)
                     /locus_tag="P5219_06185"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HRX26379.1"
                     /translation="GIGEQLIDSGFDFFAGGGLHQSTGKEKDRRDLYEVAKEKGYQVL
                     RTREDILSAKPGTKIIAVNPVLDGSNAMPYDMDRTDKELSLAEFTRKGIELLHNPEGF
                     FLMVEGGKIDWACHANDAAAAIGDTFAFDAAVKEALAFAEKHPDETLIIVVGDHETGG
                     MSIGFAGTQYETFFEKIAHQKESYEAFDRKIAAFRKESEKTFEKMMPLITGSFGLRTA
                     TEEERAALEEKARAGDDEARIALSMTLSDLELGELRTAFENSMLEKKERAKDEATFLL
                     YGGYEPLSVKITHILNRKAGIGWTSYAHTGIPVPLFAAGLGSELFAGYYDNTDVAWKT
                     MSILGVR"
BASE COUNT          418 a          680 c          784 g          431 t
ORIGIN      
        1 tttcaagagg cccgagatcc tccccgcagg ggaggaatct cggccctgaa ggaatttaga
       61 atgcctgcct gcagtataaa tccccgcacc cggttcatcc gcgatccctt ccggcgaaga
      121 gccatccgcc ggccagggcg gtgaaggggg ccacggtgac caggccgaag cttccgacga
      181 gggtgtggag tatttccgcc gagacgtact gcatgttcag gatgttggcc agggggacgc
      241 cctgggcgac gaagaccagc agcaggctgg aatagctgcc cgtgtaggcc agcagcagcg
      301 ttgtggtcat ggttcccact accgatctgc ccacggcgaa cccggacagg acaagctgcc
      361 tccgggagag gtccggccgc ttctccgcga tctcggccat ggcgctggat acgtccatgg
      421 cgaggtccat caccgcaccg gaggacgcca ggaatattcc ccccaggaag atgcgggtca
      481 ggtcgaggtg ggcgaagccg ctgtagagca gggtctccga aaaaggcttc acagcgccgt
      541 ggatgcggaa ttcgctgccg aaatagaggg agagaccgca ggtcagcagg atcccaaggg
      601 cggatcccag aaaggccgcc attcccttcc ggctgacccc gcccacgaga aagacgatgg
      661 cggcggtgag ggcaagggcg gtaaaaaggg agatgagtac cgggtcgagt ccggcgaggt
      721 acccgggaag cagcaccttc cagatcatgg cgccggtgaa gaagaaggag agcagggccc
      781 gggcccccgt ccacccgccg aagagaagga ggagggcgca gaagagtgcc agcagccgga
      841 gctgggtgtc cagccggtaa tggtccagga cgctggcctt ggcgatccgc ccggcgtcag
      901 cgtccaccac ggcaaaggca atgtcgccgg gaacgaagac cttgtccagc tccagccgac
      961 ccgccaggtg gttcacagcg tcggtttccg ttcccctgaa gctcccttcc aggatccgga
     1021 tgcggagccc ctggtcaccg gtccgcacga tgccgaactg ccgcatgttc gagttgtccg
     1081 tcgacaggac gcggaccttc acccgctccg ccgaatcggg agcctgtttc caggagggaa
     1141 gaaggaagag ggccagggtg agcagcagaa agagcagggt cagcaggtgg tcccgccgtt
     1201 tcgattccat gaaataacct ccccaataga gaaaggccgg agggagtcgc ctccggccgg
     1261 ccttgtggtg tgagtttccg gtgtgtctac cgcacgccga ggatggacat ggtcttccag
     1321 gcgacgtccg tgttgtcgta gtatccggcg aagagctcgg agccgaggcc cgctgcgaag
     1381 agaggaacgg ggatgccggt gtgggcgtag gaggtccagc cgatgcccgc cttcctgttc
     1441 aggatgtgtg tgatctttac cgagaggggc tcgtagccgc cgtagagcag gaaggtggct
     1501 tcgtccttgg cccgttcctt tttctcgagc atgctgtttt cgaaggccgt ccggagttcg
     1561 cccagttcga ggtcggagag ggtcatggag agggcgatcc gggcttcgtc gtcgccggcc
     1621 cgggcctttt cttcgagggc ggcgcgctct tcctcggtgg cggtgcggag cccgaaggac
     1681 ccggtgatga ggggcatcat tttctcgaag gtcttttcgc tttccttgcg gaaggcggcg
     1741 atcttcctgt cgaaagcctc gtaggactcc ttctggtggg cgatcttctc gaagaaggtt
     1801 tcgtactggg ttccggcgaa accgatgctc attccgcctg tttcatggtc tcccacgacg
     1861 atgatcaggg tctcatcggg gtgcttctca gcgaaggcca gggcttcctt cacggccgcg
     1921 tcgaaggcga aggtgtcgcc gatggctgca gccgcgtcgt tcgcgtggca ggcccagtcg
     1981 atcttgccgc cctcgaccat aaggaagaac ccttcagggt tgtggagcag ctcgatgccc
     2041 ttgcgggtga attcggcgag ggagagttcc ttatccgtcc ggtccatgtc gtagggcatg
     2101 gcgttgctgc cgtcgaggac cgggttcacc gctatgatct tcgtgccggg cttcgccgag
     2161 aggatgtctt cccgggtgcg gaggacctgg tagcctttct ctttcgcgac ctcgtacagg
     2221 tctctgcgat ccttctcctt gccggtgctc tggtgcaggc cgccgccggc gaagaaatcg
     2281 aacccgctgt cgatgagctg ctctccgatg ccg
//
LOCUS       DAOTHD010000316         2312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_55041_length_2312_cov_3.447940, whole genome shotgun sequence.
ACCESSION   DAOTHD010000316 DAOTHD010000000
VERSION     DAOTHD010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55041_length_2312_cov_3.447940"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..>2312
                     /locus_tag="P5219_06190"
     CDS             84..>2312
                     /locus_tag="P5219_06190"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HRX26380.1"
                     /translation="MDPKELSESILGERQVMPEEEGRVREDEHPELDRKASGKLRDSY
                     IGRIPEEERAISVKFAWHELWSKVIHYLSRDELMTLGEALVLAGAAHKEQKRSTGDPY
                     IVHSINVASILAEMQLDVVTLAAALLHDVLEDTDITIEKLKSAFGAEVATLVDGVTKL
                     GKLPFKSFEDYQAENLRKMFVVMAKDIRVVLIKLADRLHNMRTLGALRKDKQMRIARE
                     TLEIYAPLAHRLGIYQVKRGLEDLAFKYADPEMYYEIRRRVRKKLPERETVVRKAMEI
                     LSARLEEEGIHCKVKGRAKHFFSIYEKMNRKKLPVEQLYDLLALRVVVDDIAACYTVL
                     GIVHTVWKPIPGQFDDYIANPKSNMYQSLHTTVVGPTGEPLEVQIRTWEMNSLAEYGI
                     AAHWRYKEGGGGTDDLDAKLTWIRQALEGDHDGTPEPSEFLERLKDDVLSSDVFVFTP
                     QGEVVSLPKGSTPIDFAYAVHTQVGMRCVGAMVNNRIVAMGYELQNGDIVKIITSPQG
                     APSRDWLKIARSSKARSKIRAHFRALEKTERIEKLQRGRDLLERELRRRGTPEFESFE
                     ELLPLLNKIARDTGQSNGEDLLAAIGAGNQSVSLVAQRLAGKAQPQAAPLPAVPEQEK
                     HTSFAGKKGESDIVVEGAEGVQVVLSNCCEPVPGDPIVGFSTRSRGITVHRTDCENVK
                     NAKEERIISVSWGTTGNRRYTARLKLEAIDRGGLFGDVAQAIIAGDGAMTGIKAGVVG
                     GNL"
BASE COUNT          527 a          679 c          722 g          384 t
ORIGIN      
        1 attactggca gggccgggag agactgcagt acgtggttga ttacgtcgtc gttgacgaca
       61 tcacctgacg gagcgtggac ggaatggacc cgaaagaact gagcgaaagc attctcgggg
      121 aacggcaggt gatgcctgag gaggaagggc gcgtccggga ggacgaacac ccggagcttg
      181 accgaaaagc gtccggcaag ctgagggaca gctacatcgg ccggattccc gaagaggagc
      241 gggccatctc ggtgaagttc gcatggcacg aactctggtc caaggtgatc cattaccttt
      301 ccagggacga gctcatgact ctcggcgaag ccctggtcct tgccggggcg gcccacaagg
      361 aacagaagcg ttccaccggg gacccctaca tcgtccacag catcaacgtt gcttccatcc
      421 tcgccgagat gcagctcgac gtggttaccc tcgcggcggc cctccttcat gacgttctcg
      481 aagatacgga catcaccatc gaaaaactga aaagcgcctt cggggcggag gttgccaccc
      541 tcgtcgacgg agtgaccaag ctcggcaagc ttcccttcaa gtctttcgaa gactaccagg
      601 cggaaaacct ccggaagatg ttcgtggtca tggctaagga catccgtgtg gttctcatca
      661 agctggccga caggctgcac aacatgcgca ccctgggagc gctccggaag gacaagcaga
      721 tgcggattgc cagggagacc ctggaaattt acgctcccct ggcccatcgg ctcggtattt
      781 accaggtgaa gcgggggctc gaggatctcg ctttcaagta cgccgatccg gaaatgtatt
      841 acgagatccg gagaagggtc cgcaaaaaac tgcccgagcg ggagaccgtg gtccgaaagg
      901 ccatggaaat tctcagcgcc cggctggagg aagagggaat ccactgcaag gtaaagggcc
      961 gggcgaagca cttcttcagt atctatgaaa agatgaaccg gaaaaaactg ccggtggagc
     1021 agctctatga tctccttgcg ctccgggtgg tggtggacga tatcgccgcc tgctacaccg
     1081 tgcttggcat cgtccacact gtctggaaac ccatcccggg tcagttcgac gattacattg
     1141 ccaacccgaa gagcaatatg taccagtccc tccacaccac ggtggtgggg cccacgggag
     1201 agcccctgga agtgcagatc cgcacctggg agatgaactc cctggcggaa tacggcatcg
     1261 ccgcccactg gcgctacaag gaggggggag gcggaaccga cgacctcgat gccaagctga
     1321 cctggatccg ccaggccctc gagggtgacc atgacggaac ccccgaaccc tcggagtttc
     1381 tcgaacggct gaaggacgac gtgctctcaa gcgacgtctt cgtcttcact ccccagggag
     1441 aagtggtttc ccttccgaag ggctccactc ccatcgactt cgcctatgcc gtccacaccc
     1501 aggttggcat gcggtgcgtc ggggccatgg tgaacaacag gatcgtggcc atggggtacg
     1561 aactccagaa cggagacatc gtcaaaatca tcacgtcccc ccagggggcc ccttcgaggg
     1621 actggctgaa aatagcccgg agcagcaagg ccaggagcaa gatccgtgcc catttccgtg
     1681 cgctggaaaa gacggagcgc attgaaaaac tccagagggg cagggacctc ctcgaacggg
     1741 aactccgcag gagggggacg ccggagttcg agtccttcga agaacttctt cccctgctca
     1801 acaaaatcgc ccgggatacg gggcagtcga acggagaaga cctgctggcc gccatcgggg
     1861 caggcaacca gagcgtttcc ctcgtggccc agcgtctcgc gggaaaggcc cagccccagg
     1921 cggctcctct ccccgcagtg ccggaacagg agaagcatac gtccttcgcc gggaaaaaag
     1981 gggagtccga catcgtcgtc gaaggggcgg aaggagtcca ggtggtgctc tcaaactgct
     2041 gcgagcctgt tcccggcgat cctatcgttg gcttttccac ccgctcgagg gggattacgg
     2101 tccacaggac ggactgcgaa aacgtgaaga acgccaaaga agaacggatt atctccgttt
     2161 cctggggaac cacggggaac aggcggtaca ccgcccgact gaagctcgag gccatcgaca
     2221 ggggaggtct tttcggcgac gtggcccagg ccatcatcgc cggagacggc gccatgacgg
     2281 gcatcaaggc cggagtggtg ggggggaacc tc
//
LOCUS       DAOTHD010000317         2312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_55051_length_2312_cov_2.882144, whole genome shotgun sequence.
ACCESSION   DAOTHD010000317 DAOTHD010000000
VERSION     DAOTHD010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55051_length_2312_cov_2.882144"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="P5219_06195"
     CDS             complement(<1..535)
                     /locus_tag="P5219_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRX26381.1"
                     /translation="MTDARIALVTGAGKGIGRAVALRLAAMGFLVAVNYNRSEKPANE
                     VCRLIRDGGGTAVPFKADVSSGSDVKTLFSDVERELGPVAVLVNNAGITKDGLLMRMG
                     SEDWDSVLTANLSSVYHCTKEAIRGMVRAKWGRIINISSVVALIGNPGQANYCASKAG
                     IIGFTKAVAREYAGKGIT"
     gene            complement(537..1484)
                     /gene="fabD"
                     /locus_tag="P5219_06200"
     CDS             complement(537..1484)
                     /gene="fabD"
                     /locus_tag="P5219_06200"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRX26382.1"
                     /translation="MTYALVFPGQGSQETGMGKDFYDTFSSARDVFNRADQALGFSLS
                     AVIFNGPEEELRRTAFTQPAILTVSIAVLECLRRETGIPRAPLFLAGHSLGEYTALVA
                     SGVLSLEDGVRLVHLRGRLMQEAVPEGEGAMAAILGLAPESVAAVCSEAAPEGECQPA
                     NFNSPGQVVISGSTEFVKAAMDKAKEAGAKKTVLLNVSAPFHSRQMRPVADKLQEAFS
                     SMSWKEPSVPIVSNALASPVHSVAEIQKALFDQTFMPVLWQDSVVRMAEDGVDTFWEI
                     GPGSVLTGLVKKCRKGVAAQACSSVPEFEALSNKLKEMV"
     gene            complement(1497..>2312)
                     /locus_tag="P5219_06205"
     CDS             complement(1497..>2312)
                     /locus_tag="P5219_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRX26383.1"
                     /translation="ANMPPDLLEIQLQKVRTLTDKPFGVNIMLMSPTVDQVIDLVAGY
                     RVPIVTTGAGSPGKVIEILKPLGTIIVPVIASVAQAKRVEKQGADAVVAEGMEAGGHI
                     GELTTMVLIPQIVDSVEIPVIAAGGIADGRGVAAAFALGAEGVQVGTRFICCEESTVH
                     RAYKEAVLNARDRSNVVTGRFTGHPVRCLKNKLTSKFEELEKSGATVEEYERLGAGKL
                     RAAVVDGDVEWGSVMAGQSAALVNDILPAAEIITELFSGAERVVDSLPGRNSR"
BASE COUNT          475 a          699 c          619 g          519 t
ORIGIN      
        1 ccgtgatgcc cttgccggcg tattcccgcg ccacggcctt ggtgaaaccg atgatgcccg
       61 ccttggaggc acaatagttt gcctgcccgg gatttcctat gagggcgacc acagaagaaa
      121 tattgatgat ccttccccac ttcgcccgaa ccattccgcg tatcgcttcc ttcgtgcaat
      181 gatacaccga ggagagattc gctgtcagca cggaatccca atcttcgctt cccattctca
      241 tgaggagtcc gtctttcgtt attcccgcgt tgttgacgag tacggcaacc ggcccaagtt
      301 cgcgttcgac atcggaaaaa agggtcttca catcgcttcc ggaagaaacg tcggccttga
      361 aaggaacggc agttccgcct ccgtccctga tcagccggca tacttcgttt gcaggctttt
      421 cgctccggtt gtaattcact gcgaccagga aacccatcgc agcaagacga agggcaaccg
      481 ctctgccgat tcccttgccc gcacctgtta caagtgcaat cctggcatca gtcattctac
      541 accatctcct tcagtttgtt tgaaagagcc tcaaattcgg gaactgaact gcaggcctgg
      601 gctgcaacac cttttctgca tttctttaca agcccggtga gtaccgaacc ggggccgatt
      661 tcccagaaag tatccacacc gtcctcagcc attctgacga ccgagtcctg ccagaggaca
      721 ggcataaagg tctggtcgaa aagggccttt tgtatttcgg cgaccgaatg tacaggagag
      781 gcaagggcat tggagacaat gggtacagac ggctccttcc agctcatgga gctgaaggct
      841 tcctgcagct tgtccgcgac gggccgcatc tgcctgctgt ggaagggggc gctgacgttc
      901 aggagaactg ttttttttgc ccctgcctct tttgccttgt ccatggctgc cttgacgaac
      961 tcggtgctgc cggaaataac cacctgcccg ggggagttga agttcgccgg ctgacactcg
     1021 ccttcgggag ccgcttcaga acagacggca gcgacagatt ctggcgcaag tcccagaatg
     1081 gcggccatgg cgccttctcc ctccggtacc gcctcctgca tgagcctgcc ccgaaggtga
     1141 accagccgga caccgtcttc gagggaaagg acaccggaag ccacaagagc agtatactcg
     1201 ccgagactgt gtccggccag gaaaagagga gcccgaggga tgccggtttc ccgtctgaga
     1261 cattccagaa cggctatgct gacggtgaga attgccggct gggtaaaggc tgttctcctc
     1321 agttcttcct caggaccgtt gaagatgaca gccgacagcg aaaacccgag tgcctggtcg
     1381 gcgcggttga aaacatccct tgccgaagag aaggtatcgt aaaaatcctt ccccatcccg
     1441 gtttcctggg atccctggcc cggaaagacc agcgcatagg tcataaccct gtcacctcac
     1501 ctggaatttc ttccgggaag ggagtcgaca acccgctccg ctccggaaaa gagttccgtt
     1561 ataatctcgg ccgcaggaag gatgtcgttt acaagagcag cgctctgccc cgccatgacg
     1621 gacccccact ccacatcacc gtctacaacg gcggcccgga gtttccccgc gccgagtcgt
     1681 tcgtactctt ctaccgtggc tccgcttttt tcgagttcct cgaatttaga agtcagcttg
     1741 tttttcaggc agcggacggg atgtcccgta aatcggccgg tcactacgtt gcttcggtcc
     1801 ctggcattga ggacggcttc cttgtaggcc cgatgcacgg tggattcctc gcagcagata
     1861 aaacgggtcc cgacctgaac gccttccgcc ccaagagcaa aggctgccgc cactcctctc
     1921 ccgtcggcaa tgccgcctgc cgctatgact ggtatttcca cggaatcgac aatttgggga
     1981 ataagcacca tagttgtgag ttcaccgata tgtccgcctg cttccattcc ctcggctacc
     2041 actgcgtcag ctccctgttt ttccaccctt ttcgcctggg cgacagaggc tataacggga
     2101 acgattatcg tcccgagggg cttgaggatc tcaatgacct ttccggggct tcccgctccg
     2161 gtggtaacga tgggtaccct gtatccggca acgaggtcga taacctggtc tacggtgggt
     2221 gacatgagca ttatgttgac gccgaagggt ttatccgtca gggtcctcac cttttgcagc
     2281 tggatctcga gaaggtccgg cggcatattc gc
//
LOCUS       DAOTHD010000318         2297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_55562_length_2297_cov_2.676628, whole genome shotgun sequence.
ACCESSION   DAOTHD010000318 DAOTHD010000000
VERSION     DAOTHD010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55562_length_2297_cov_2.676628"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="P5219_06210"
     CDS             <1..676
                     /locus_tag="P5219_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRX26384.1"
                     /translation="LDRAETSVLIAVFSMLLIILVGGPIGILSAVRYNSRLDQLFSGL
                     SMLFASVPTFWLGLNLMLLFSVMLRWLPTSGFPSILESGDWSNLRYLVLPCITLAAPN
                     SALIIRLTRSSMLDVAKADYVKTARAKGLSEGAVNIRHIFRNSLISVVSALGFTFVAL
                     MAGTVVTETVFSLPGIGRLVVESVLRRDYPTIQGIILIIAFLYMVINLLVDLSFALLD
                     PRIRYS"
     gene            689..1528
                     /locus_tag="P5219_06215"
     CDS             689..1528
                     /locus_tag="P5219_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRX26385.1"
                     /translation="MKFLSAFYRRKIALGGLLFIVLLLIFALFAPYIATFDPQEMNPV
                     DKLRSPDGTYYFGTDNFGRDIFSRVVFGARTSLAGSAGVILFSLSIGVLIGVSSGYFR
                     RIEPYLMRLIDVLMAFPPLLLALVLVSILGRGMGNVIIAVGATYLTRTTRIVYGLTLK
                     LREEPYIEAAVASGVKQKWIIMKHILPNMMSPLIVQATFTFAFSLLDMAALDFLGLGI
                     PPSIPSWGNMLSEGRMYLTRAPWLLVFPGACIVLTVLSFNLVGDVLRDRLDPHFRRDI
                     EGV"
     gene            1532..>2297
                     /locus_tag="P5219_06220"
     CDS             1532..>2297
                     /locus_tag="P5219_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX26386.1"
                     /translation="MEPETNTSLLEIRDLRVSFPSASGHIFPVDGVSLSIAKGETLGL
                     VGESGSGKSMTSLAVMGLVPRPHGKICGGSIAFKGRNLVELTNEEMMNIRGKDISMIF
                     QEPMTSLNPVYTVGDQIVEGILAHGAMSRREARDRAVEMLAKVGIPGPEKRVDSYPHL
                     LSGGMRQRVMIAMALSLDPELLIADEPTTALDVTIQAQILDLMDGLKKERTMSVLLIT
                     HNLGIVAESAQIVAVMYAGRILELASTEALFRDPLHP"
BASE COUNT          413 a          705 c          655 g          524 t
ORIGIN      
        1 cctcgaccgg gctgaaacaa gcgtccttat tgccgtcttt tccatgctgc tcatcattct
       61 cgttgggggg cccataggaa tcctttcggc cgtccggtac aacagccggc tcgaccagct
      121 tttttccggt ctttccatgc tcttcgcgtc ggtgcccacc ttctggctcg ggctgaacct
      181 gatgcttctt ttctcggtga tgctgcggtg gcttcccacc tcagggttcc cttccatcct
      241 ggaatcgggg gactggtcga acctccgcta tctggttctt ccctgcatca ccctggccgc
      301 tcccaattcg gccctcatca tccgcctgac gcggtcgagc atgctggacg tggccaaggc
      361 ggattacgtg aagactgccc gggcaaaagg attgtccgag ggagccgtca atatacggca
      421 tatcttccgg aattcgctca tctccgtcgt atccgccctt gggtttacgt tcgtggccct
      481 catggcggga accgtagtga ccgaaacggt cttctccctt ccggggatcg ggcggctcgt
      541 ggtggaatcg gttctgagaa gggactatcc caccatccag ggaatcattc tcatcatcgc
      601 ttttctgtac atggtgatca atctcctggt ggacctgtcc tttgctcttc tggatccgcg
      661 gatccggtac agctaggagg ggcggaccat gaaatttctt tcggcctttt acaggagaaa
      721 aattgcactc ggcggattgc ttttcattgt tctgctcctg atttttgccc ttttcgcccc
      781 gtacatcgcc accttcgatc cccaggagat gaatcccgtg gacaagctcc gctcccctga
      841 cggaacctac tatttcggga ccgacaattt cgggagggac atcttcagcc gggtagtgtt
      901 cggagcccgg accagcctgg ccggatccgc cggggtcatt cttttttcac tttccatcgg
      961 cgtgctcatc ggcgtatcgt cggggtactt cagaaggata gagccgtacc tgatgcgact
     1021 tatcgacgtc ctcatggcct ttcctccgct gcttcttgcc ctggtgctcg tttccatcct
     1081 cgggcggggc atggggaacg tgatcatcgc cgtgggggcc acctatctta cccggaccac
     1141 ccgtatcgtc tacggcctga ccctgaagct ccgggaggaa ccgtatatcg aagccgccgt
     1201 cgcttcggga gtgaagcaga aatggatcat catgaagcat attctgccca acatgatgtc
     1261 acccctcatc gtccaggcca cgttcacctt cgccttttcc ctcctggaca tggcggccct
     1321 ggactttctc gggctcggca ttccgccgtc catcccgagc tggggcaaca tgctcagcga
     1381 ggggcggatg tacctcacga gggcgccgtg gctccttgtt ttcccggggg cctgcatcgt
     1441 cctgaccgtc ctttccttca accttgtggg agacgttctc cgcgaccggc ttgacccgca
     1501 cttccggcgg gacatcgagg gggtgtgagc catggagccg gaaacaaata catccctcct
     1561 tgaaatacgg gatctcaggg tcagcttccc ttcggcttca gggcacattt ttccggtgga
     1621 cggcgtgagc ctctccatag cgaagggaga aaccctcggg cttgtggggg agtctgggag
     1681 cggcaaatcc atgacctccc tggcggtgat gggccttgtg ccccgccccc acggaaagat
     1741 ctgcggcggc agcatcgcgt tcaagggcag aaacctggtg gagctgacca acgaagagat
     1801 gatgaacatc cggggaaagg acatttccat gatcttccag gagcccatga cgtccctgaa
     1861 ccccgtgtac acggtgggag accagatcgt ggagggcatc ctggcccatg gggcgatgtc
     1921 acgaagggaa gcccgggaca gggccgtcga gatgctggcg aaagtgggca ttccgggacc
     1981 ggaaaagcgg gtggattcct accctcatct tctgagcgga ggaatgaggc agcgggtgat
     2041 gatcgccatg gccctctccc tggacccgga gctgctcatc gccgacgagc ccaccacggc
     2101 cctcgacgtg accatccagg cccagatcct ggatctgatg gacggcctga aaaaagagcg
     2161 caccatgtcg gtgctcctca taacccacaa cctgggaata gtggcggagt cagcccagat
     2221 agtggccgta atgtacgccg gcagaattct ggagcttgct tcgacggaag ctttgttccg
     2281 agaccccctg cacccct
//
LOCUS       DAOTHD010000319         2293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_55683_length_2293_cov_2.057641, whole genome shotgun sequence.
ACCESSION   DAOTHD010000319 DAOTHD010000000
VERSION     DAOTHD010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55683_length_2293_cov_2.057641"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="P5219_06225"
     CDS             <1..542
                     /locus_tag="P5219_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26387.1"
                     /translation="AKSFRSDVLIHGKAQGNSFARQKIEGVTLFGVRSQLQLKAVITQ
                     NAVVLGTEIAEAKTKGTSPGDGAVKGFTTLAPRSAAELVHKVAYALVSGSSGGIPGRT
                     YRVSVSGVSFSEARNLREGLAGKKGITGVYQRSFADRTLEMDVTAELTSEEVALALEA
                     LGVEVTGLTGGTVEGRKHP"
     gene            539..988
                     /locus_tag="P5219_06230"
     CDS             539..988
                     /locus_tag="P5219_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26388.1"
                     /translation="MKGILPLFLLLFLLAAARGCASLSLEQIDQIRRNNGGAEGIQVF
                     RYGVVDWSGGSVTAEGRGPLLSGSPHDRLLAKRGAVSDARRNLLCLLYEMKFGLPEKL
                     ESIEVSGEVVEGNIDFQGVRNGMYIVEVTVSLDRFLSESLIFSSTVR"
     gene            985..1791
                     /locus_tag="P5219_06235"
     CDS             985..1791
                     /locus_tag="P5219_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26389.1"
                     /translation="MIFMKWRPWILLVLLVISCLLLPDAEALGAALPQGKTIAVLVRG
                     TPSHAAAVRSLLVRALLDGGYRAVDEQQLEKIRRSKAAALALEGNVDAILQLGRSFGF
                     SVLLSGAVTVPKPVRNEFGLFTATASVSVTACHAANGRQILALTESAKEIGYTPEEAA
                     QKAAEKAASSAGDVILGKRAAGQPENPASKYTVIVSPVRSFAEAHGIVESCRDAGALS
                     ASLLRFAAGKAEVEAEFGGTAAQLVAALLRVRKEFFEESAEGNVIRLGKQ"
     gene            1817..>2293
                     /locus_tag="P5219_06240"
     CDS             1817..>2293
                     /locus_tag="P5219_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26390.1"
                     /translation="MMMIHGKRIALLMLAGIQFFLVLSGSTAARAEVSLFIDAHTVRV
                     HDPSLGRQSVLNDFRTKGHRTHNYLALVWSEDAAWVYDTRTHQWLSQGDFRTLLGSLS
                     DEFALVWNDSEAAVFDAKKQEWIRSETMPWTVSGAHLSRGMAALSGDDGFVVYDPVL"
BASE COUNT          459 a          674 c          713 g          447 t
ORIGIN      
        1 tggcaaaatc cttccgctcc gatgtgctga tccacggcaa ggcccaggga aattccttcg
       61 ccaggcagaa gattgaaggc gttaccctgt tcggtgtgcg gtcccagctc cagctcaagg
      121 ccgtcatcac ccagaacgcc gtcgttctgg gaacggagat cgccgaagcg aagaccaagg
      181 ggacatcccc cggggacgga gcggtgaagg gtttcaccac cctcgctccc agatcggcgg
      241 cggaactcgt ccacaaggtg gcctatgccc tcgtgagcgg gtcttccggc ggcattcctg
      301 ggaggacgta ccgggtctcc gtatccggcg tttcgttcag cgaagccaga aacctccggg
      361 aaggcctggc gggaaagaag ggtataacgg gggtttacca gagaagcttt gccgacagga
      421 ctctcgagat ggacgtgacg gcggagctca catctgaaga ggtggccctt gccctggaag
      481 ccctcggcgt tgaggtaacc ggcctgaccg gggggaccgt tgaagggaga aagcatccat
      541 gaaagggatc ctgccgctgt tcctgctcct ctttcttctc gcggccgccc ggggatgtgc
      601 ctccctgtcc ctggaacaga tcgatcagat acggcggaac aacggcggag cggagggaat
      661 acaggtgttc cggtacggtg tcgtggactg gagcggggga tccgtcaccg ccgaaggcag
      721 aggccccctt ctctccggtt caccccatga caggcttctg gccaaaaggg gagccgtttc
      781 cgatgcccgc cggaatcttc tctgccttct gtacgaaatg aagttcggcc ttccggaaaa
      841 gctggagtcc atcgaggtat cgggagaagt cgtcgagggg aacattgact tccagggagt
      901 caggaacggc atgtacatcg tcgaagtgac cgtttcgctg gacaggtttc tctcggagag
      961 cctgattttc agcagcacgg tgaggtgatt ttcatgaaat ggcgtccatg gatactcctg
     1021 gtattgcttg tcatctcctg cctcctcctt ccggatgccg aagccctggg agccgccctt
     1081 cctcaaggaa agaccatcgc cgttctcgtc agggggacac cctctcatgc ggctgcggtc
     1141 cgttcactcc tggtccgggc ccttctcgac gggggctatc gggcagtgga cgagcagcag
     1201 ctcgaaaaaa tcaggcgcag caaggccgca gccctggctc ttgaagggaa tgtggatgcc
     1261 attcttcagc tcggccgaag tttcgggttt tccgtgcttc tctcaggcgc ggtcaccgtt
     1321 ccgaaaccgg tgcgaaatga attcggactt tttacagcca cggcctccgt ttcggtcacg
     1381 gcctgccatg cagccaacgg caggcagatt ctggccctga cggaatcggc gaaagagatc
     1441 ggctacactc cggaagaggc ggcgcaaaaa gcggcggaaa aggccgcctc gtccgcagga
     1501 gatgtcatcc tgggaaaaag agccgccgga cagccggaga acccggcgtc aaaatacacc
     1561 gtcatcgttt cgccggtgag atccttcgcc gaagcccacg gcatagtgga aagctgcagg
     1621 gatgccggag ccctctccgc ttcccttctg cggttcgccg ccggaaaggc cgaggtggag
     1681 gcggagttcg gaggaacggc ggcgcagctt gttgccgccc ttcttcgggt tcgcaaggag
     1741 ttcttcgaag agtccgcgga ggggaacgtc atcaggctgg ggaagcagtg aaacggcctt
     1801 ttcatgaaag gaagggatga tgatgatcca cgggaaaaga atcgcgctcc tgatgctcgc
     1861 agggatccag tttttcctcg ttctctccgg ctcgacggct gcacgggccg aggtcagtct
     1921 gttcatcgat gcccataccg tccgggtcca tgatccctct ctcgggcgcc agtcagttct
     1981 caacgacttc cggacaaaag gtcaccggac ccacaactac cttgcccttg tatggagcga
     2041 agatgctgca tgggtatacg acaccaggac ccatcagtgg ctctcccagg gagatttccg
     2101 gaccctgctc ggttccctct ccgacgaatt cgccctcgtc tggaacgaca gcgaggcggc
     2161 agttttcgac gcgaagaagc aggaatggat acggagcgaa accatgccct ggaccgtcag
     2221 cggagcccat ctctcccggg gaatggccgc tctctccgga gacgacggct tcgtggtcta
     2281 tgatcccgta ctg
//
LOCUS       DAOTHD010000320         2288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_55816_length_2288_cov_4.069861, whole genome shotgun sequence.
ACCESSION   DAOTHD010000320 DAOTHD010000000
VERSION     DAOTHD010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55816_length_2288_cov_4.069861"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="P5219_06245"
     CDS             <1..673
                     /locus_tag="P5219_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HRX26391.1"
                     /translation="KAESLGYTVVDAPSVLATHLSEVIKRFGADLLTRQEVQKLTDMV
                     KEAAPAVVEEMMAALSLGEIQKVLQNLVREQVPIRDLVSIFEALADYGKTSRSVDFLT
                     ERVRESLARIITLRIQDESGTVTVATLSPNWEEKVKTSMDGDLLRGWQFTMEPREVQK
                     MMGAVSRAAEDMSVKGLVPVLLVHPDVRLVMRRILEGSLPGLFVISYNEISQGAQLKS
                     VGMVE"
     gene            674..2059
                     /gene="flhF"
                     /locus_tag="P5219_06250"
     CDS             674..2059
                     /gene="flhF"
                     /locus_tag="P5219_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HRX26392.1"
                     /translation="MAIKIKRGTQITFEAKDDAEALRTARDRLGPDAVILSSRQVKTG
                     GFLGLFRRTVLQVSAAVLEEDRRSESDAEKKERLAAFQKLLEIKQAMVSPGEGVSGSE
                     STVLDGMPFRGDQALSAAGVYSPRSPKVKKEVPIPETAPDGVEFSPKGLALASEKAAP
                     AAEPPEKVSPEKLQNEVNEIARRLDLVLKRLETERSEPAPPPPPSDEGKDILAKLLAA
                     DVSETHARSLVERFRDDPQGRNFLQWLGKKISVAAQESWEALGGRKVMLIGPTGVGKT
                     TTIAKLAAIHSLWKKKKVFLLTADTYRIAAVKQVETYSKILGIPMDVVDDPRHIGPVL
                     EKTAGADLVLLDTAGRSQRDGARIEEMRALYLAFQPDAVHLVLAANMKYRDMLDVVKR
                     MGVVPISRVIFTKTDETTTYGALLNILLDFDRPVSFITTGQNVPNDIEVASGMRLAEM
                     LLGEERDAGSV"
     gene            2111..>2288
                     /locus_tag="P5219_06255"
     CDS             2111..>2288
                     /locus_tag="P5219_06255"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HRX26393.1"
                     /translation="MQRRPARAGYGLRSLAVLSGKGGVGKSNISVNLSLAMAGRGKRI
                     VLLDADLGMANVDLL"
BASE COUNT          497 a          687 c          695 g          409 t
ORIGIN      
        1 caaggccgag tccctcgggt acaccgtcgt ggacgcccct tcggtcctgg cgacacatct
       61 ttcggaggtc atcaagcggt tcggcgctga cctgctcaca cggcaggaag tgcagaagct
      121 caccgacatg gtgaaggagg cggcacccgc cgtggtggag gagatgatgg ccgccctgtc
      181 cctcggggag atccagaagg tcctccagaa cctcgtcagg gagcaggtgc ccatccgtga
      241 cctggtaagc atctttgaag cgctggcgga ctacggaaag acctccagga gcgtggattt
      301 cctcaccgag cgggtgaggg aatccctggc ccggataatc accctccgca tccaggatga
      361 gagcggcacg gtcacagttg ccaccctttc cccgaactgg gaggaaaagg tcaagacttc
      421 catggatggc gaccttctcc ggggatggca gttcaccatg gagcccaggg aagtgcagaa
      481 gatgatgggg gccgtttcga gagccgcgga ggatatgtcc gtgaaggggc tggttcccgt
      541 tctgctcgtt caccccgacg tgcgcctcgt catgcggcgg attcttgaag gatcgctgcc
      601 cggccttttt gtgatatcct ataatgaaat atcgcaggga gcacagctta agtcggtcgg
      661 gatggtggaa taattggcta ttaaaatcaa gagagggacc cagatcacct tcgaagcgaa
      721 agatgacgct gaagctctcc ggaccgccag ggaccgtctc ggtcccgatg ccgtcattct
      781 ctcctcccgg caggtgaaga caggaggctt tctcgggctc ttccgccgca ccgtcctcca
      841 ggtgagcgcc gcggtcctcg aggaggaccg ccgttccgag agcgacgcag aaaagaaaga
      901 acggctggcg gccttccaga aactcctcga gatcaagcag gccatggttt cacccggtga
      961 gggggtctcc ggcagcgaat ctacggttct cgacggaatg ccattcagag gagaccaggc
     1021 tctttccgcc gccggcgtct attccccccg ctccccgaaa gtgaagaaag aggttccaat
     1081 tccggagacc gcccctgacg gcgtggagtt ttcgccgaaa ggccttgccc tggcatctga
     1141 aaaagcggct ccggcggctg aaccgccgga aaaggtctcc ccggaaaaac tccagaacga
     1201 agtcaacgaa atagcccgaa ggctcgacct tgtcctgaag cgccttgaaa cagagcgctc
     1261 cgaacctgcc ccgcctcccc ctccttccga cgaggggaag gacatcctgg cgaagcttct
     1321 tgccgcggac gtgagcgaaa cccacgcccg ttccctggtg gagcggttca gggacgaccc
     1381 ccagggaagg aattttctcc agtggctggg caaaaagatc tccgtggctg cccaggagtc
     1441 ctgggaagcc ctcgggggac ggaaggtcat gctcatcggg ccaaccggcg ttgggaaaac
     1501 caccaccatc gccaagcttg cggccattca ctccctgtgg aaaaagaaaa aagtttttct
     1561 gctcactgcc gacacctaca ggattgcggc ggtgaagcag gtcgaaacct attccaagat
     1621 cctgggaatc cccatggatg ttgtggacga cccaaggcac atcggccccg tactggagaa
     1681 gaccgccggg gcggaccttg ttctgctcga caccgcaggc cgttcccaga gggacggagc
     1741 acgcatcgag gaaatgcggg cgctctatct tgccttccag ccggatgcgg ttcaccttgt
     1801 cctggctgcg aacatgaaat accgggacat gctcgacgtg gtgaaacgga tgggagtggt
     1861 ccccatttcc agggtcatct tcaccaagac cgacgaaacc accacctacg gtgcccttct
     1921 caatatcctt ctggactttg accgccccgt ctccttcatc accacgggac agaacgttcc
     1981 caacgatatc gaggtcgcat cgggaatgcg gctggcagaa atgctcctcg gggaagagag
     2041 ggacgccggc agtgtctgag gttgaaacca gaaacggaga ccaggcgaaa gaccgcgccg
     2101 catagtcgac atgcagcgac gacccgccag ggcggggtac gggcttcgat ccctcgctgt
     2161 cctgagcggc aagggagggg tcgggaaaag caacatctca gtgaaccttt ccctggccat
     2221 ggccgggaga ggcaaacgga tcgtccttct tgacgccgac ctcgggatgg ccaacgtgga
     2281 tctgctct
//
LOCUS       DAOTHD010000321         2279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_56131_length_2279_cov_3.527878, whole genome shotgun sequence.
ACCESSION   DAOTHD010000321 DAOTHD010000000
VERSION     DAOTHD010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56131_length_2279_cov_3.527878"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..1123
                     /locus_tag="P5219_06260"
     CDS             188..1123
                     /locus_tag="P5219_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HRX26394.1"
                     /translation="MLMQEKISVSLDLFFDHLRFEKEASPHTVTNYSVDLAQFAEFLE
                     VQGIDAPEEVEGKTIRSWLREMMGYGYAKTSAARKLSSVRSWFAFLFDRGLIPSDPAK
                     GVRGPKLPSRLARALSREDAARLVEAGQSGEDPLRDSAVLELLYGCGLRIAELAALRW
                     QDVDLDERMVRVLGKGSKERIVPFGTCALRALGSWRDSAETENGHVFPGQRGGAIAVR
                     TVHRIVRRAALKAGVANVTPHVLRHSFATHLLEGGASLRVLQELLGHESLLTTQHYLA
                     VGADHLRKSYELAHPRAKGDNGDVQGNDDSLCPEE"
     gene            1083..1616
                     /gene="hslV"
                     /locus_tag="P5219_06265"
     CDS             1083..1616
                     /gene="hslV"
                     /locus_tag="P5219_06265"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HRX26395.1"
                     /translation="MFKGTTIVCVRKNDKVAMAGDGQVTLGNQIIKAGARKVRRLHKG
                     TVLAGFAGSTADAMTLLERFESRLEENSGDLMRSAVSLVKEWRTDRALRRLEALMIVA
                     DSGLTLMLSGAGDILEPENNVAAIGSGSGYAQAAALAFLESSGMPPSQIARRSIEIAS
                     EICIYTDNIVTVEALGE"
     gene            1613..2279
                     /locus_tag="P5219_06270"
                     /pseudo
     CDS             1613..2279
                     /locus_tag="P5219_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048897.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT          493 a          638 c          746 g          402 t
ORIGIN      
        1 gctcgcggga actgccgaat agagataatc cctccgtttt ttctcttatt tttctctccc
       61 acgggatggg agaaaagagg aacaggaatg aaaaatttca gtcagggaca ggatttttac
      121 ccctgatgaa aaaacattaa ttgattcttt tgaaaaatat cgagaatcta gaaaaagatg
      181 ctaaaatatg ctcatgcagg aaaagatatc cgtttccctc gatttatttt tcgatcacct
      241 ccggtttgaa aaggaggcgt cgcctcacac ggtgaccaac tactccgtgg atctcgccca
      301 gttcgccgag tttctcgagg tgcagggcat agacgccccg gaggaggtgg aaggaaagac
      361 catacgatcc tggcttcggg agatgatggg atacggctac gcgaaaacgt cggctgccag
      421 gaagctttcc tccgtccgga gctggttcgc cttcctgttt gaccgggggc tgattccgtc
      481 cgatcccgcc aaaggggtcc gggggccgaa actcccctcc aggctggcca gggctctctc
      541 ccgggaggac gccgcacggc tcgtcgaggc gggacaaagc ggcgaggacc ccctccggga
      601 ctcggcggtg cttgaactcc tgtacggatg cggtctccgg atcgccgagc tcgcagccct
      661 gcgctggcag gatgttgacc tggatgaacg gatggtccgg gttctgggca agggaagcaa
      721 ggagcggatc gttcccttcg gaacgtgtgc cctccgggct ctcgggagct ggagggattc
      781 cgcggagacg gagaacggcc atgtcttccc cggtcagcgg ggaggagcca ttgcggttcg
      841 gactgtccac cgcattgtgc ggagggcggc cctgaaggcc ggggtggcga acgtgacgcc
      901 ccacgtgctg cggcacagct tcgcgaccca cctcctcgag ggaggggcgt ccctcagggt
      961 gctccaggag cttctcggtc acgagagcct gctcaccacc cagcattatc tcgccgtcgg
     1021 ggcggatcac ctgcggaaaa gctatgagct ggcccatccc cgggcgaaag gagacaacgg
     1081 agatgttcaa gggaacgacg atagtctgtg tccggaagaa tgacaaggtg gccatggccg
     1141 gcgacggcca ggtgaccctc ggaaaccaga tcataaaggc gggagcgaga aaagtccgcc
     1201 ggctccataa aggaacggtc ctcgccggtt tcgccggaag caccgccgat gccatgacgc
     1261 tcctggagcg gttcgaaagc cgcctcgagg agaactcggg cgacctcatg cgctccgccg
     1321 tgtcgctggt gaaggaatgg cggaccgaca gggcccttcg gaggcttgaa gccctcatga
     1381 tcgtcgccga ttcggggctc accctgatgc tctcgggcgc gggggacatc ctggagccgg
     1441 agaacaacgt ggcggccata ggctccgggt ccggctacgc ccaggcggcg gccctggcgt
     1501 ttcttgaatc aagcggcatg ccgccgtccc agattgcccg gagatccatc gagattgctt
     1561 cggagatctg catctatacg gacaacatcg tcacagtgga ggctcttgga gaatgaccag
     1621 aaatgagaat gaccggttcg acctcactcc gcgccagatc gtggagtacc tcgaccggta
     1681 tatcgtggga cggaaaaggc aaagaaatct gtggccatcg ccctgagaaa ccgcatacgg
     1741 agacggcgcc ttcccgagaa tatcgccaag gaagtggcgc cgaagaacat tctcatggtg
     1801 gggcccacgg gcgtgggaaa gacagagatc gcccgacgtc tcgccgatct tgtgaaagcc
     1861 cccttcgtca aggtggaggc caccaagttc accgaagtag gctacgtggg gcgggacgtg
     1921 gaatctatcg tgcgggacct ggccgagacg gccgtggcca tggtgaagaa gcggaagatc
     1981 gaggacgtcc gggttcccgc ggcggagcgg gcggagcagc gtttggcgga tgccctgctg
     2041 ccccggccgg agaagaaaag cgccgttccc gatttcatgc gcctcttcgg gggcggagga
     2101 tcaccggaaa agaaccagga acgggatgat tcgcccgaag aagagaggct ccgggattca
     2161 accaggagaa aaattcttgc cctcctccgg gaggggaagc tcgatgacag ggaagtggag
     2221 atcgacgtca ccgaaagcca gtcggtgggc ataccccttc tcgggggagc cggaatgga
//
LOCUS       DAOTHD010000322         2275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_56270_length_2275_cov_3.355856, whole genome shotgun sequence.
ACCESSION   DAOTHD010000322 DAOTHD010000000
VERSION     DAOTHD010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56270_length_2275_cov_3.355856"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="P5219_06275"
     CDS             complement(<1..182)
                     /locus_tag="P5219_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HRX26396.1"
                     /translation="MAKTATKGLTGREKVAILMVALGNEVAAEIYKRLDDATIEIVTL
                     EIANLRKVTPEQRLGVL"
     gene            complement(194..1765)
                     /gene="fliF"
                     /locus_tag="P5219_06280"
     CDS             complement(194..1765)
                     /gene="fliF"
                     /locus_tag="P5219_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HRX26397.1"
                     /translation="MDWFRRFSARLGLFWSSLKPWQKLSIVGASILVVSALVLAAYWA
                     GKPSYEPLFSALEVEDQSAIVAYLRENRIDYKLDPAANAILVPRDIVYETRLSLAQGG
                     LPKGGSVGYEIFDTTKMGLSEFQQKITYIRALEGELERTIRQIDAVDYAKVNIVIPEQ
                     RLFLEQQQPSTASVLVRLKTGMQIGPDQVKSIVHLVSHGVQGLLPENITVVDTTGKVL
                     SDLLDQDLLIYGQGGDGRTVSSVQRELERQQERELENKVRVMLERVFGPGRVVVRIKV
                     DLDFDRRTSSSKEFIPGETGRGVLRSQQNMEETYTGTGGPIGGAPGTTTNIPGYAVAQ
                     QQGTASEYNKTETTNNYEITTRETQQVVTPGTIRRLSASVLVDGDLEQDRLTELRALV
                     APALGVDAARGDQLVIQSMKFSTTFADSLAEQLQAQQRMQLIIALVAAAVILLLAALA
                     GVLWMKRRRSRKAMLPPAEERKRIPSIQELLSSPELLEAQGELAVLEEQLRAYARSKP
                     EEIANLTQDWLADEA"
     gene            complement(1778..2095)
                     /gene="fliE"
                     /locus_tag="P5219_06285"
     CDS             complement(1778..2095)
                     /gene="fliE"
                     /locus_tag="P5219_06285"
                     /inference="COORDINATES: protein motif:HMM:NF014143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HRX26398.1"
                     /translation="MGSLHIDLLKLASPSMVSGPETVSKKAPAGGTGLSFEELLQDSM
                     KRVNVLQVESDDLVNKLATGDVDDISRVVLASQRAEFALRMITEVRNKLLDAYQQLGR
                     MPV"
BASE COUNT          398 a          678 c          694 g          505 t
ORIGIN      
        1 agcactccga gccgctgttc gggggtcacc tttctcaggt tcgcgatttc aagggtgacg
       61 atctcgatgg tggcatcgtc gagccgcttg tagatttctg ccgccacttc gtttccaagg
      121 gccaccatga ggatggccac tttttccctt cctgtcagtc ccttggtcgc tgtcttcgcc
      181 atggctacgc ctcctatgcc tcgtcggcaa gccagtcctg ggtaaggttg gctatttctt
      241 caggtttgct tctcgcgtag gcccgaagct gttcttccag tacggccagt tctccctggg
      301 cctcaagaag ttccggcgac gaaagaagtt cctggatgct cggtatgcgc ttccgttcct
      361 ctgccggagg gagcatggct ttcctggatc tgcgtctttt catccacagc accccggcga
      421 gggcggcgag gagaagaatg accgcggcgg cgacaagggc gataatcagc tgcattctct
      481 gctgggcctg cagctgttcc gccaggctgt cggcaaaggt ggtggagaat ttcattgact
      541 ggatcacgag ctggtctccc ctggccgcgt ccactcccag ggcgggcgcc acgagggccc
      601 ggagttccgt cagcctgtcc tgctcaaggt cgccgtccac gaggaccgag gccgaaaggc
      661 gccggatggt gccgggggtc accacctgct gggtctcccg ggtggtgatc tcgtagttgt
      721 tcgtggtctc cgtcttgttg tattcgctgg ccgtcccctg ctgctgggcg acggcatagc
      781 cggggatgtt cgttgtggtc cccggtgccc ctccgatggg cccacccgtt ccggtgtagg
      841 tttcttccat gttctgctgg ctccggagga ctcccctgcc cgtttctccg gggataaatt
      901 ccttcgagga actcgttcgc cggtcgaagt caaggtcgac cttgatcctc accacgaccc
      961 gcccgggacc gaacacccgc tcgagcatga cccggacctt gttttcgagc tcccgttcct
     1021 gctgacgctc aagctcccgc tgaacagagg agactgtccg gccgtccccg ccctggccgt
     1081 agatgagcag atcctggtcg agcaggtccg aaaggacttt tcccgttgtg tccaccaccg
     1141 tgatgttttc gggaagcagg ccctggacgc cgtgggacac gagatgcaca atggatttca
     1201 cctggtcggg tccgatctgc atccccgtct tcaaacggac gaggaccgag gcggtggaag
     1261 gctgctgctg ctcgaggaag aggcgctgct cggggatgac gatgttcacc ttggcatagt
     1321 ccacggcgtc gatctggcgg atggtccgtt caagctctcc ctcgagagcc cgtatgtagg
     1381 tgatcttctg ctggaattcg ctcaagccca tcttcgtggt gtcgaagatc tcgtagccca
     1441 cgctcccccc tttcggaagg ccgccctgag ccaggctgag ccgggtttca tagacgatgt
     1501 cccggggaac gaggatggcg ttcgccgccg ggtcgagctt gtagtcgatc cggttttccc
     1561 ggaggtaggc cactattgcc gactggtcct ccacttcgag ggcggaaaaa agaggttcat
     1621 aggagggttt gcccgcccag taggcggcaa ggacaagggc ggagaccacc aggatggagg
     1681 ccccgacgat ggagagcttc tgccacggct tgagggaaga ccagaaaagt ccgagacggg
     1741 cggagaacct tcggaaccag tccattcacg cgggccctta cacaggcatc cggccgagct
     1801 gctggtaggc gtcgagcagt ttgttcctga cctcggtaat catccggagg gcgaactccg
     1861 ctctctggga agcgagaacc acccgggaaa tgtcgtccac gtccccggtg gcgagcttgt
     1921 tcaccaggtc gtcggattca acctggagaa cgttgaccct cttcatggaa tcctgaagaa
     1981 gttcctcgaa ggaaagaccg gttccgccgg caggggcctt ttttgaaacc gtctccggtc
     2041 cggaaaccat cgacggtgaa gcaagcttca agaggtctat gtgaagggaa cccatgggag
     2101 cctctcctcc tttgtccata aatattcgta ccagtgcccc tatattagca cacgggttct
     2161 atttataaca aaaacgggag gtgcatgcgt gcgcctcccg tctgttgttc catgggccgg
     2221 aagtcctttg tcttcaggaa cttcccgtgt gcggcggtac cagagggctg gaata
//
LOCUS       DAOTHD010000323         2274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_56324_length_2274_cov_2.460117, whole genome shotgun sequence.
ACCESSION   DAOTHD010000323 DAOTHD010000000
VERSION     DAOTHD010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56324_length_2274_cov_2.460117"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="P5219_06290"
     CDS             complement(<1..467)
                     /locus_tag="P5219_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRX26399.1"
                     /translation="MSKYVFVTGGVVSSLGKGITAASLGVLLKKRGLRVSIIKLDPYL
                     NVDAGTMNPFQHGEVFVTDDGAETDLDLGHYERFIDESLCADNNVTTGKIYSSVISKE
                     RRGDYLGGTVQVIPHITNEIQERILRAGEGRDVVIAEIGGTVGDIEGQPFLEA"
     gene            complement(585..>2274)
                     /locus_tag="P5219_06295"
     CDS             complement(585..>2274)
                     /locus_tag="P5219_06295"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HRX26400.1"
                     /translation="GGLSDSDGPEEFVLPSSGFIVVDMSSVYLSDDGRELSHRSLKPG
                     GSQGVNGAAAGADREWSFPDSVVVSAGGDGVSGSLSANSGSSRLSGFRLKMPEQIPVE
                     GEAFGLSSESLSSAGMSRLSGFRIPLPENLPVEGTLFGTGGADVANSAEFRLGAFAVS
                     TPASEAAPVSPGTVPPDLGSLMTGKMESDYDSVVLSTASDGKSLPAEEDPELSAEEED
                     VVLTEVKPLWKEHIVKAGETMSDIAVSYGVSMSDILKANELKDPNRLSEKQLLLVPNS
                     GDAVDATLEEVMTRKARVLAAQEKVVPVKVTAYVVAKGDSLWSIANSQNLEIDSLFGC
                     NDLKNPDLLKPGVTLRIPNQDGIFYTVKKGDTLEKIAKSYGIPVDRIRKGNAAESLSP
                     LQEGREVFLPGARPEAADSAPAQKSGSAKSRPKSEGRTVSSASRSYRWPVMGKISSPF
                     GWRRHPVTRRRDFHTGIDVRAPRGRAIKAAKAGRVEYAGWMGGYGRVVVVRHNDGLST
                     LYAHCNSLSVRNGQSVSQGQTIASVGTSGRTTGPHLHFEIRQGKSPMNPLKLLR"
BASE COUNT          430 a          717 c          669 g          458 t
ORIGIN      
        1 atggcctcga ggaagggctg accctcgatg tcgccgacgg tgccgccgat ctcggcgatc
       61 accacgtccc gtccctcacc ggcccgaagg attctttcct ggatctcgtt ggtgatgtgg
      121 ggaatgacct gcacggtccc cccgaggtag tcccccctcc gctccttgga gatgacggag
      181 gaataaattt ttcccgtggt cacgttgttg tccgcacaga gggactcgtc gatgaaccgc
      241 tcgtagtgtc caaggtcaag gtctgtctcc gcgccgtcgt cggtcacgaa gacctcgccg
      301 tgctggaagg ggttcatggt tcccgcgtcc acgttcagat aaggatcgag cttgataatg
      361 gagaccctga gtccccgctt tttgagaagc accccgaggg acgccgcggt aattcccttg
      421 cccaacgaag aaacaacccc gccggtgaca aaaacgtact tcgacatttc ctccatcctc
      481 cacgataaag aaatttacgg cagacagcat taaaaaaaca tggaccaggg aattccccgg
      541 tccagaatag cgaaattgtc acggaatgtc atggtcacta ttgcctatct taacagcttc
      601 agagggttca tggggctctt tccctgccgg atctcgaagt gaagatgggg tccggtggtc
      661 ctgccggagg ttcccaccga cgcgatggtc tgtccctggg agacggactg gccgttccgc
      721 accgacaggg aattgcagtg ggcgtagagg gtggaaagac catcgttgtg gcggacgacc
      781 acgaccctgc cgtatccccc catccagccg gcgtattcca cccggcccgc cttcgccgcc
      841 ttgatcgccc tgcctcttgg ggccctcacg tcgatgcccg tatggaagtc cctcctgcgg
      901 gtcaccgggt ggcgcctcca tccgaagggg ctgctgatct tccccatcac gggccaccgg
      961 tagcttctgg atgccgaaga aaccgtcctg ccttcgctct tcggccggct cttcgcggaa
     1021 ccgctcttct gtgccggcgc ggaatcggcg gcttcaggac gggcaccggg caggaacact
     1081 tcgcgccctt cctggagtgg gctcagcgat tcagccgcgt tccccttccg tatcctgtcc
     1141 acgggaatgc cgtagctctt ggcgattttc tccagggtat cgcccttctt caccgtgtag
     1201 aaaatgccgt cctggttcgg gatccgcaga gtcaccccgg gcttcagaag gtcggggttc
     1261 ttcaggtcgt tgcatccgaa aagggagtcg atctcgaggt tctgggagtt tgctatggac
     1321 cacaggctgt cccccttggc caccacgtag gcggtgactt tcacgggaac caccttttcc
     1381 tgggcggcga ggacacgggc cttccgggtc atcacttctt caagggttgc atccacggca
     1441 tcgccgctgt tggggacgag aagaagctgc ttctcggaga gcctgttggg gtccttcagt
     1501 tcattggcct tcagaatgtc gctcatggaa acgccgtagg acacggcgat gtcggacatg
     1561 gtctccccgg ccttgacgat atgctctttc cagaggggtt tcacctccgt gagcaccacg
     1621 tcctcctctt ccgccgaaag ctcggggtct tcctcggcgg gaaggctttt gccgtccgat
     1681 gccgtgctca gcaccacgga atcgtaatcc gattccatct tgccggtcat caggctcccc
     1741 aggtcgggag gaacagttcc tggagataca ggtgccgctt cagacgcagg cgtgctcacg
     1801 gcgaaggctc cgagacgaaa ctccgcggaa ttggccacat ctgccccgcc ggtcccgaag
     1861 agggtgcctt ccacagggag gttctccggc agggggatgc ggaatcccga aagccgggac
     1921 attcccgcgc tggaaaggga ttcggaggaa agcccgaagg cttccccctc cacgggaatc
     1981 tgctccggca tcttcaggcg aaagcctgaa agacgggagg aaccggaatt ggcggacaaa
     2041 gaaccggata ctccgtcgcc gccggcactc accacgacac tgtcggggaa gctccactcc
     2101 ctgtcggcac ctgcggccgc gccattcacg ccctgcgagc cgccgggctt cagggaccga
     2161 tggctcagtt ccctgccgtc gtcggaaagg tacacgctgg acatatccac cacgataaag
     2221 cccgaagagg gaaggacaaa ttcctccgga ccatcggaat cagagaggcc gccc
//
LOCUS       DAOTHD010000324         2273 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_56348_length_2273_cov_2.929666, whole genome shotgun sequence.
ACCESSION   DAOTHD010000324 DAOTHD010000000
VERSION     DAOTHD010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56348_length_2273_cov_2.929666"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            680..1675
                     /locus_tag="P5219_06300"
     CDS             680..1675
                     /locus_tag="P5219_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HRX26401.1"
                     /translation="MKKALFLLVCLAFIASGAMSMAAEVEGDGKSYELMVSTQLADSH
                     PFCQGFYALAKRVAERTGGKLTVKVFPSAQLGSDEDVIEQAIQGVNVAVVTDAGRMAN
                     YVKNIGIVGMAYFADNYDEVMKVQQTNFWKENVKKLAEENGIRVLSFNWFDGARHFLT
                     NKPVKKPEDLKGVRIRTPGAPAWSRSVAALGATPVTMGWTDVYNAVQQKAIDGCEAQH
                     SASYGLRVYEVLKYINKTAHFQLLNGLIVGEKWFSTLPASYQALLLEEFEKQGKITAA
                     EVIEKSDVFEGMMVKEGMEVVEVDLAAFKKASEAAYDELGFAEQRKAIYKEIGKE"
     gene            1787..>2273
                     /locus_tag="P5219_06305"
     CDS             1787..>2273
                     /locus_tag="P5219_06305"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRX26402.1"
                     /translation="MLEALKKTYRCVCTIEERFISFTLCAITVLIFVSAIGRFVGRPI
                     NWAVDISLLLFAWGAFLGADVGIRKNRVINVDFLTSRLPLKTQKTLAVAWSVIIILFL
                     AVLIAYGIPLCISNFKRQFQNITLSYSFVTASLPVSAFLMIISTGIRLHLQITDFPST
                     LR"
BASE COUNT          490 a          692 c          622 g          469 t
ORIGIN      
        1 ggccgaaatc tcctccagga gggagaagaa aggctcgctt cgatggctga ccaggggtgc
       61 ggcgcctctc tgccgtattt cgtggggtat ggagaccggt ccgggggtga aaagatgggg
      121 ctgcatggat gtatccctcc ttcgaaatga aaaaggccgg aagggatttc ccttccggcc
      181 tgtatttcct gcgatcgaat cctgcgtttc tgtcgcggtg tcttgatcta aggctgcggc
      241 gccttccccg ccggcacggg aagaccagcc cttccggacc agccggaaga ggaggaggaa
      301 gatgaccccg taccaagaat cccgaaaaga cacacaaagt tcataagcgg cagctccccg
      361 aaaaaaaagt tgttcacttt atatcagcac aacctatcaa tgtcaatagc ttttcctgaa
      421 tttctctccg gaaaaaaagg acattgcctc ctgggccgca catatccggg ccgcacatat
      481 cttgacaaaa ttccgcccga tgcctaagat actaacatat cagaccgtac attcgggcta
      541 ctttgaattg cttgattttt tgttccgaac tgctcccgtt caaaaaaagg ttctccgccg
      601 gcctcttttc ccggggaatc catcgtccgg attaccggcc gccaggcccg taattaatac
      661 attcagggag gaatgaaaca tgaagaaagc gttgttcctg ctggtatgtc ttgcgttcat
      721 cgcttccggt gccatgtcca tggccgcgga ggtggagggg gacggaaaat cctacgagct
      781 catggtgtcc actcagctcg ccgattccca tcctttctgc cagggctttt acgccctcgc
      841 gaagcgcgtg gccgagagaa ccggcggcaa gctgaccgtg aaggtgttcc ccagcgccca
      901 gctcggcagt gacgaggacg tcatcgagca ggcgatccag ggcgtcaacg tggcggtggt
      961 caccgacgcc ggacggatgg ccaactacgt gaagaacatc ggcatcgtgg gcatggccta
     1021 cttcgccgat aactacgacg aggtcatgaa ggtccagcag acgaacttct ggaaagagaa
     1081 cgtcaaaaag ctggcggaag agaacggcat ccgtgtcctc tccttcaact ggttcgacgg
     1141 cgcccgccac ttcctcacca acaagcccgt caaaaagccc gaagatctga aaggcgtccg
     1201 gatccgcacc cccggcgctc ccgcttggtc acggtcagtg gccgctctcg gcgccacccc
     1261 tgtcaccatg ggatggaccg acgtgtacaa cgccgtgcag cagaaggcca tcgacggctg
     1321 cgaggcccag cattccgcca gctacggcct gcgcgtgtac gaagtcctga agtacatcaa
     1381 caagaccgcc cacttccagc tcctcaacgg actcatcgtc ggagagaagt ggttcagcac
     1441 ccttccggcg tcctaccagg cccttcttct cgaggagttc gagaagcagg gaaagattac
     1501 cgccgccgaa gtcatcgaga agtccgacgt cttcgagggc atgatggtca aggaaggcat
     1561 ggaggtcgtg gaagtggatc tggcggcctt caagaaggct tccgaggcag cctacgacga
     1621 actgggcttc gccgagcagc ggaaggcaat ctacaaggaa atcggcaagg aataatttcc
     1681 ggccggaata tcctgcgccg tacggtcgat tcaccggcag ccgccgcctt tccggggcgg
     1741 ttgcctttcc ctttttcccg gcgccggaag gaggctgcac acggacatgc ttgaagctct
     1801 gaaaaaaacc taccgctgcg tctgcacgat cgaggagcgg tttatttctt tcaccctttg
     1861 cgccatcacc gtcctgattt tcgtttccgc cataggcagg ttcgtcggcc gccccatcaa
     1921 ctgggcggtg gacatttccc tcctcctgtt cgcctggggt gccttcctcg gggcggacgt
     1981 ggggatccgg aaaaacaggg tgatcaacgt cgatttcctg acgtcgcggc tgcctctgaa
     2041 gacgcagaaa accctcgccg tcgcgtggtc cgtgatcatc atcctcttcc tggcggtgct
     2101 catcgcctac ggaatccccc tctgcatctc aaatttcaaa cggcagttcc agaacatcac
     2161 cctgagctat tccttcgtca ccgcatccct tcccgtgagc gccttcctga tgatcatctc
     2221 cacgggcatc aggcttcatc tgcagatcac cgacttcccg tccaccctcc ggg
//
LOCUS       DAOTHD010000325         2273 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_56356_length_2273_cov_2.371957, whole genome shotgun sequence.
ACCESSION   DAOTHD010000325 DAOTHD010000000
VERSION     DAOTHD010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56356_length_2273_cov_2.371957"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..612
                     /locus_tag="P5219_06310"
     CDS             61..612
                     /locus_tag="P5219_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRX26403.1"
                     /translation="MVWTALLSAVAAVLMYIDMAVPLFPRFLKLDLSDLPALVAAFAW
                     GPAAGVFTELIKNLIHSLTTSTAGVGELANFLMGSALVVPAGLIYRRNRTRKGALAGL
                     AAGTLSMTVTAALANYFFLLPFYSNFLPLEKIIAMSSAAIPAVRDMGTLVLYAVVPFN
                     LLKGTVVSLATVQLYKRISTLIR"
     gene            732..2201
                     /locus_tag="P5219_06315"
     CDS             732..2201
                     /locus_tag="P5219_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26404.1"
                     /translation="MNRSAAALLCVLWLGLPAPNAWSAPKVPPGSGSGAVLQAVPVSL
                     SVLSRIGQKVSLSGFYYGGSIPMVIDDISRTDYNMELPAGCFVPLSGPRPSGLKSGDR
                     ITVSGVLERPGKTSSLKNCPAVLKISSLENVRIIAPRILPPATMKPVKPEMLRRLEKY
                     RPGDLVPGRKFAVLIAGGGGPENNHIRYWNDLLTMYNILLGRGFAAADIFVFYADGAP
                     PGDVEGKIEGSMPVNGTASRTNIQRCFRTIGTVSRDNDFIFIMTNDHGGGFLEVRTGS
                     LDPGLYGGRVASAGEANDSISEAQFSLDLNGDGDTTDVLRADESLNMWTGRYYDDDFA
                     ADLNRISRYGTMVIHLEQCFSGGFLDDLRAPRRIIFSATSEQRVSRAHSASPAHPRAV
                     QPYNEIFYWFAAALLGETPDGDYAVDRNGAPWTPNADYDGNGKVSVFEAFMFARRMNL
                     KDGLPWYEDNNRPGCITVNVDTRNVPEGRDGWLGSRTFF"
BASE COUNT          411 a          752 c          689 g          421 t
ORIGIN      
        1 ttttcttttt cgggaggcga attcttcatg gaagcgacga cggtcaactc aacccgccgc
       61 atggtctgga ccgcccttct gtccgccgtg gcggcagtgc tcatgtacat cgacatggca
      121 gttcccctgt tcccccgttt tctcaagctc gacctgtcgg accttccggc cctggtggcc
      181 gccttcgcgt ggggaccggc tgcgggggtc ttcaccgagc tgatcaagaa cctgatacat
      241 tcgctgacca cgtccaccgc cggggtgggc gaactggcga attttctgat ggggagcgcc
      301 ctggtggtcc ctgcgggctt gatctatcgg cggaacagga cccggaaagg cgccctcgcg
      361 gggctggcgg cgggcaccct gtccatgacc gtcacggcgg ccctggcgaa ctacttcttt
      421 ttactcccct tctattcgaa ctttctcccc ctggagaaga tcatcgccat gtcctcggcg
      481 gccattcccg cggtccgcga catggggaca ctggtgctgt acgccgtggt gcccttcaac
      541 ctcctcaagg ggacagtggt ctccctggcg acggtccagc tctacaagcg catcagcacc
      601 ctcatccggt aaaaacctat ctgcagggag tgtcaccggc gcccgccggg gccgccggac
      661 tcaacaccca caaaaagacg aaaaagactg tataatcttt ccatataccc tcatcggagg
      721 tggtcgggaa gatgaaccgt tccgcggctg cgctgctctg cgtgttgtgg ctggggctcc
      781 ctgccccgaa tgcctggagc gccccgaagg ttcctcccgg aagcggctcg ggggcggttc
      841 ttcaggccgt tcccgtgtcc ctttccgtcc tgtcccggat cgggcagaag gtctctctgt
      901 ccggttttta ctacggcgga tccatcccca tggtcatcga cgacatctcg cggaccgact
      961 acaatatgga gctcccggcg ggctgcttcg tccctctgtc gggtccccgg ccgtcggggc
     1021 tgaagtccgg cgacaggatc accgtctcgg gcgtgctgga gcggccggga aagacatcct
     1081 ccctgaagaa ttgccctgcg gtgctgaaga tttcctctct tgaaaacgtc cggatcatcg
     1141 cgccccgcat tctgcccccc gcaacaatga agccggtgaa accggaaatg ctccggagac
     1201 tggagaaata ccggcccggc gacctcgttc cgggacggaa gttcgccgtt ctcatcgccg
     1261 gagggggcgg gccggagaac aaccacatcc gctactggaa cgacctgctc accatgtaca
     1321 acatccttct gggcaggggc ttcgccgccg ccgacatttt cgtgttctac gccgacggcg
     1381 cccctccggg cgacgtggag gggaagatcg aaggctccat gccggtgaac ggcacggcaa
     1441 gcaggacgaa catccagcgg tgcttccgga ccatcgggac cgtgtcgcgg gacaacgact
     1501 tcatcttcat tatgaccaac gaccacgggg gaggttttct cgaggtacgg accggctccc
     1561 tcgacccggg actgtacggc gggcgggtcg cctcggccgg ggaggcaaac gattccattt
     1621 ccgaggcgca gttcagcctc gacctgaacg gcgacggcga cacaacggac gtgctgcggg
     1681 ctgacgagag cctgaacatg tggaccggcc ggtattacga cgacgacttc gccgccgacc
     1741 tgaaccggat ctcccggtac ggcaccatgg tgatccacct ggagcagtgc ttctccggcg
     1801 gctttctcga cgacctccgg gctccccgga ggatcatctt ctccgccacg agcgaacagc
     1861 gggtctccag ggcccattcg gcgagtcccg cacatccccg ggccgttcag ccctacaacg
     1921 agattttcta ctggttcgcc gccgccctcc tcggggagac acccgacgga gactacgccg
     1981 tggaccggaa cggagccccc tggaccccga acgccgatta cgacggcaac ggaaaggtca
     2041 gcgtgttcga ggccttcatg ttcgcccgga ggatgaacct gaaggacggc ctgccctggt
     2101 acgaggacaa caaccggccg ggctgcatca cggtaaacgt ggacacccgg aacgtgcccg
     2161 agggaaggga cggatggctc ggcagcagga ccttcttctg agaaacagcc ttctcccccg
     2221 gaaaaacgat tcccgataaa agccccggcc ggcggccggg gcttttatca gga
//
LOCUS       DAOTHD010000326         2265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_56597_length_2265_cov_3.294118, whole genome shotgun sequence.
ACCESSION   DAOTHD010000326 DAOTHD010000000
VERSION     DAOTHD010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56597_length_2265_cov_3.294118"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1870)
                     /locus_tag="P5219_06320"
     CDS             complement(<1..1870)
                     /locus_tag="P5219_06320"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HRX26405.1"
                     /translation="MMNLTEKEFLEELRHDFILEAEEYLQTIAAGLLDLERGDPPPRV
                     MEEVFRAAHSLKGAAQAVRMPAISGLCQVLESVFSAMKKGVLHPSPEGFDILQRSVDA
                     LAAMVAAGGEDDGRGAEAGKALEIILTDEAPSGSVFPPVFPRRKETAGAGTETVEKKM
                     EPLPASFSASEDHGREGQERSAGRSASRETVRIGTDKLDGLLLRAEEMISLNQVLDVR
                     LGEMRDLRHLLSEWLGEWRRTENVLKKTGEYKQLPSRAAEFFTAGRDRLREAWERAAA
                     MERSLADNGRTSGGLVADLLDEARSVLMLPFSSLLQGFPKIVRDLARGLLKDVEFSAE
                     GGDVEVDKRILEGLKDPLVHLVRNALDHGIERTEERRAAGKPEKGRVSLAVSSVERGR
                     VEIVLSDDGKGIDSERLRESAVASGFLKAEEAGALSGEEALMLIFQSGLSTSPVITDI
                     SGRGLGMAIVREGVEKLGGKISLSSLPGKGTRFTISLPVTLATFRGVLVREWGHQFVI
                     PTACVRRAARVLRSDIRTVENRETISLDGTPHALVRLGTVLGLAEPSGGAEKDILPVV
                     AAGTGETCVAFAVDGVVNEQEVLLKPLGGILKRVRNVGGVTVLGSGKVVPVLHCGDL"
     gene            complement(1899..>2265)
                     /locus_tag="P5219_06325"
     CDS             complement(1899..>2265)
                     /locus_tag="P5219_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26406.1"
                     /translation="KQSAREVQRILRDIQKATGAAVMAIEQGSKAVDRGAKEATPSRE
                     SVLALSRRFTESAQSAAQIAAANNELLSGIGQVAQAMENIREAGEQNVTGMKELESAA
                     ANLRDMGRRLAALVEGYRI"
BASE COUNT          377 a          777 c          649 g          462 t
ORIGIN      
        1 ccaggtcgcc gcagtggaga accggaacga ccttcccgga accgagcact gtcactcccc
       61 ccacgttcct cacccgtttg aggattccgc ccagtggttt gagaagtact tcctgctcgt
      121 tcaccactcc gtccaccgcg aaggcaacac aggtttctcc tgttccggcg gccacaaccg
      181 gcaggatatc cttctccgct ccgccggagg gttcggcaag gccgagaacg gtgcccaggc
      241 ggaccagggc atggggcgtt ccgtcaaggg atatcgtctc cctgttttcc accgtcctga
      301 tgtcggaacg cagcacccgg gccgccctcc gcacacaggc cgtgggaatg acgaactggt
      361 gcccccattc tctcacgagt actccccgga aggtggcaag agtcaccgga agggagatgg
      421 tgaaccgtgt cccttttccc ggcagggagg aaaggctgat tttcccgccg agtttttcca
      481 ctccctcccg gacgatggcc atgccgaggc ccctgccgga gatgtccgtg atgacggggc
      541 tggtggaaag cccggactgg aatatgagca tgagagcctc ttcgccggaa agagccccgg
      601 cttcttcggc cttcaggaac cccgatgcca cagcgctttc cctgagcctc tcagaatcga
      661 ttccctttcc gtcgtcggag aggacgatct ccacccggcc tctctccacc gaagagaccg
      721 cgagggacac ccttcccttt tcgggcttgc ctgcggcccg ccgttcctcc gttctttcga
      781 tgccgtggtc aagcgcgttc cggacaaggt ggaccagggg atccttcagc ccctcgagaa
      841 tgcgcttgtc cacctccaca tccccgcctt cagcggaaaa ttccacgtct ttgaggagac
      901 cccgggcaag gtcccgcact attttgggaa atccctggag cagggaggaa aaggggagca
      961 tgaggacgga ccgggcctca tcgagaagat ccgccaccag gcccccggat gtcctgccgt
     1021 tgtccgccag ggagcgctcc atggcggcgg cccgttccca tgcttcccgg agacggtccc
     1081 gccccgccgt gaagaactcc gccgccctgg aggggagttg tttgtactct ccggtcttct
     1141 tcaggacatt ctccgttctc cgccattccc ccagccactc cgaaagaagg tgccggaggt
     1201 ccctcatttc gcccagccgg acgtcgagaa cctggttcag ggagatcatt tcctccgccc
     1261 ggaggagcag cccgtcgagc ttgtcggtcc ctatcctgac cgtttccctg gaggcgcttc
     1321 ttccggccga tcgttcctgc ccctcccttc cgtggtcttc tgaagcggaa aaggatgccg
     1381 gtaagggttc catctttttt tcaacggtct ccgtccctgc cccggctgtt tcctttctcc
     1441 ggggaaaaac cggggggaaa acggagcccg aaggggcctc atcggtcaaa atgatctcca
     1501 gggccttccc ggcttccgcg ccccgaccgt cgtcctcccc gccggcggcc accatggccg
     1561 ccagggcatc aaccgagcgc tgcaggatgt cgaatccttc aggtgacgga tggagcaccc
     1621 cttttttcat ggcggaaaaa acgctttcca ggacctggca gagccccgaa atcgcgggca
     1681 ttcgtaccgc ctgggcggct cccttgaggc tgtgggcagc ccgaaagacc tcctccatga
     1741 cccggggagg cgggtccccc cgctccaggt ccaggaggcc ggctgcaatg gtctggaggt
     1801 attcctccgc ttcgaggatg aaatcgtgtc tgagctcctc gagaaattcc ttttccgtca
     1861 ggttcatcat gccgctcctc cggggaactg cctcccggtc agatccggta cccttcgacg
     1921 agggccgcca gccttcttcc catgtccctc aggtttgccg cagccgattc gagctccttc
     1981 attcccgtca cgttctgctc acccgcttcc cggatgtttt ccattgcctg ggccacctgg
     2041 ccgatcccgg aaagaagttc gttgttggct gcagcgatct gggcggcgga ctgggcggat
     2101 tccgtaaacc tcctggaaag ggcgaggacc gattccctgg agggagtcgc ctccttcgcg
     2161 ccgcggtcca ccgccttgga tccctgctcg atggccatca ccgccgcacc ggtggccttc
     2221 tggatatcac ggaggatcct ctggacttcc ctggcggact gtttc
//
LOCUS       DAOTHD010000327         2265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_56604_length_2265_cov_2.864253, whole genome shotgun sequence.
ACCESSION   DAOTHD010000327 DAOTHD010000000
VERSION     DAOTHD010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56604_length_2265_cov_2.864253"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="P5219_06330"
     CDS             <1..469
                     /locus_tag="P5219_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRX26407.1"
                     /translation="DKERLAVTGGSYGGYMTNWIIGHTDRFRAAASQRGISNWISKMG
                     ISDIGYYFVPDQQGADIWSDHEKLWWHSPLKYADRAKTPTLFIHSEEDHRCELTQGLQ
                     MFTALRLHGVETEICVFRGENHELSRSGRPRPRLTRLRKMTEWFDRFLAAGEE"
     gene            complement(529..1053)
                     /locus_tag="P5219_06335"
     CDS             complement(529..1053)
                     /locus_tag="P5219_06335"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRX26408.1"
                     /translation="MSTVLALFWSFLKIGCVGYGGGPSMVPLIKEEVVNIQQWMSVTD
                     FVDMLAIGNALPGPIATKLSVVIGFDVAGPLGSIAALAGTVLPSVVVLILLLRFVAMI
                     SGNPKVTSMLKGLRPVVVALLAYAAYDMSFGSLTGPLTWAIGGVTLLMMIFTKIHPAL
                     FVVAGAVAGALLGL"
     gene            complement(1107..1634)
                     /locus_tag="P5219_06340"
     CDS             complement(1107..1634)
                     /locus_tag="P5219_06340"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRX26409.1"
                     /translation="MGKDLFALFVSFAKTGSVAYGGGPSMVPLLKAEVIERRNWIGTE
                     DFMDALAIGNALPGPIITKMSAAIGYRKAGWPGALVAIAGIILPSALALLVLMGFVSL
                     VKDNPLVTSMLRGLRPVVVAMLAYAAWDMAPNALKNRTTWVICGVSLALMVFSPIHPA
                     LIIVAGAVAGIALKL"
     gene            complement(2061..>2265)
                     /locus_tag="P5219_06345"
     CDS             complement(2061..>2265)
                     /locus_tag="P5219_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26410.1"
                     /translation="SVVLMSGKGESIAFMFSIGEESAMNKYESDMMDLIKSFAMEGSS
                     SGSSSGKTTPRPQSRPAPQSGGS"
BASE COUNT          482 a          738 c          642 g          403 t
ORIGIN      
        1 cgacaaagag aggctggccg tcaccggggg gtcctacgga ggctacatga ccaactggat
       61 catcggccac acggaccgct tcagggccgc cgcatcccag aggggcatca gcaactggat
      121 ctccaagatg ggcatttccg acataggcta ctacttcgtc ccggaccagc agggcgccga
      181 catctggagc gaccatgaga agctctggtg gcactccccg ctgaaatacg ccgaccgggc
      241 gaagaccccc accctcttca tccattccga ggaggaccac cggtgcgagc tcacccaggg
      301 actgcagatg ttcaccgccc tcaggctcca cggcgtggag accgaaatct gtgtcttccg
      361 gggcgaaaac cacgagctca gccggagcgg ccggcccagg cccaggctta cccgcctgcg
      421 gaagatgacc gagtggtttg acaggttcct cgccgccggg gaagaatagc acaaggggca
      481 ggccgcgggg cctgcccctt tttattccgc tttccgcccg gtctgcatct acagcccgag
      541 cagggccccc gccactgcgc ccgccaccac gaacagggcg ggatgaatct tcgtgaaaat
      601 catcatgagc agggtcacac cccctatggc ccaggtgagg ggtcctgtca gggagccgaa
      661 ggacatgtca taggcggcat aggcgagcag ggccaccacc actggacgga gccccttcag
      721 catgctcgtg accttcgggt tgccgctgat catggcaaca aaccgcagga gcaggatcag
      781 gacgacgacg ctcggcagca ccgtcccggc caaagctgcg atcgacccca gcggaccggc
      841 cacgtcgaac ccgatcacta cggacagttt tgtcgctatg ggtcccggaa gggcgttccc
      901 gatggcgagc atatccacga aatcggtgac ggacatccac tgctggatgt tcaccacttc
      961 ctccttgatg aggggaacca tggacggccc gccgccgtat cccacgcacc ctattttcag
     1021 aaaagaccag aacaatgcca ggaccgtgct catgtcatcc tctcctcttc gcttcctgta
     1081 tgcggcaccc catcatcgcc cgcctcctag agtttaaggg caatgcccgc cacggccccg
     1141 gcaacgatga tcagggccgg gtgaatgggg gagaaaacca tcagcgccag ggacaccccg
     1201 cagatgaccc aggtggtccg gttcttcagg gcgttcggag ccatatccca ggccgcgtag
     1261 gcaagcatgg ccaccaccac ggggcgaagt ccccgcagca tgctcgtcac cagcggattg
     1321 tccttcacaa gggagacaaa ccccatcagg accagcaggg caagggcact cgggagaatg
     1381 atgcccgcaa tggccacaag ggcaccgggc caaccggcct tcctgtagcc gatggcggcc
     1441 gacattttcg tgatgatggg tcccggaagg gcattgccta tggccagggc atccataaaa
     1501 tcctccgtgc ctatccagtt ccggcgctcg atcacttccg ccttcagcag aggcaccatg
     1561 gacggccccc cgccgtatgc cacggagccg gtcttcgcaa aggacacgaa cagggcaaaa
     1621 agatcctttc ccacaacaac tcctccttca ttccatttcc tgatgtacgg aaaaacgttc
     1681 ccgcgaaggc gattctcgcg ggaacgccgg cttcaattcc ttattgcatc ttgcatttcc
     1741 gattgtagat gatcctcgtt tttttttcaa tcaggccggt tttcccggat aacacccggc
     1801 agcgtccatc ccggaagaga gacgtccctg tgatttcacc gccctgaaag cgcagccccc
     1861 gctttttccc agggcaggat cgcaggagaa gtcgccggag gcggcttttc agaggcacgg
     1921 cacaagggta tcctattatg gctgaagggg caatcgcggc ggaaaaaagg agcctccgtt
     1981 caccggaggc tcttgaagaa tcattgaccg tgctgcccgt ttctctcaaa aaccccccac
     2041 gggggttttc atcagccccc tcagctgccg cccgactggg gcgccggccg ggactgcgga
     2101 cggggcgtcg tctttccgga cgaagaaccc gaagagcttc cttccatggc aaaggacttg
     2161 atcaggtcca tcatgtcgga ttcatacttg ttcatggcgc tttcctctcc gatggagaac
     2221 atgaaggcga tgctctcgcc ctttcccgac atgaggacaa cgctg
//
LOCUS       DAOTHD010000328         2265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_56611_length_2265_cov_2.681448, whole genome shotgun sequence.
ACCESSION   DAOTHD010000328 DAOTHD010000000
VERSION     DAOTHD010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56611_length_2265_cov_2.681448"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2265
                     /locus_tag="P5219_06350"
     CDS             <1..>2265
                     /locus_tag="P5219_06350"
                     /inference="COORDINATES: protein motif:HMM:NF018208.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26411.1"
                     /translation="GDGGFQRLRIVRQGDLLFSGSVSIPPATKTEVALFIRQDGTPGA
                     GSCVLKRSFSPETGAVITVDGARTRLSDLDAVKRLWKLEGDQFAFIGQGEVAEAIRQR
                     PAQRRAHLELLFGIDQYRRKRNETSAKLVSAEEESLRLGALVAELNNRREEIAPAVAL
                     ASRARAITDGLEEKRRLFYFHRRRVLEESLAALEEEIRTLGAEETFRIRWRDLWEKMY
                     SRSLRERGELEEREKCLRGDREELLRKREDLRRSCFASAAAVREIRSRMGSLEGEEKT
                     LSARLEEMGAEWGEILSKENDLAAGLAAAVEERDRLRRRMNELRAAIEKEQERKKNLS
                     AALSALGAEKETLRSKRESREAFLADCDARIRTSGEILAALQEESAALEEKIAGLERR
                     EAEVVEAHGDAFAACRKTASALQQARKESASLEAAAEDLKTAESSSYPEPVRFLTSAY
                     RLGKLQVPVTVAAESFSCPPSVTSALEAYLGGRQYWIFVKTLAEAGACIDLLKERRAG
                     RATFLPLEKSRPRFPDRRAVLPARGVVGWAIEIVTPSEEWESCVSHLLGDLLLVEEYS
                     VGARLVSGGASYPIASLEGEVFLPSGTVSGGRTRAAAGAIERRRRIREAEERLARVRQ
                     EGAALAAALEKEEALERARSAEKEAISLELREKRKRLEEKRRDMDLERAAGERLDRDR
                     LQALDDFASWEKRMAEIDGEMEVLSASMPSTAEGDEESSLPARLTEAENSCVLLEERL
                     SSVSSLRNRAASEL"
BASE COUNT          452 a          686 c          768 g          359 t
ORIGIN      
        1 tggcgacggg ggattccaga ggctgcggat cgtcaggcag ggggacctgc ttttttccgg
       61 gagcgtgagc attccccccg cgacgaagac ggaagtggcc cttttcatcc ggcaggacgg
      121 aactcccggc gccggaagct gcgtcctcaa gcgatccttt tcaccggaaa ccggagcggt
      181 gatcaccgtg gacggtgccc ggacgaggct ctcggatctc gatgcagtga agaggctctg
      241 gaagctcgag ggcgaccagt tcgccttcat cggccagggg gaggtggctg aggccatccg
      301 ccagaggcct gcccagagaa gggcccatct cgaacttctc ttcggcatag accagtaccg
      361 ccgcaaaagg aacgagactt cggcaaagct cgtctctgcg gaggaggaaa gcctccgtct
      421 gggggccctt gtggctgaac tcaacaaccg cagggaagag atcgctcccg ctgtcgccct
      481 cgcatcccgg gcacgggcca taaccgacgg gttggaggaa aaacggcggc tcttttattt
      541 ccaccggagg cgcgttctcg aagaaagcct tgcggccctt gaggaggaaa tccgtaccct
      601 cggcgcggaa gagaccttcc gcatccgctg gagagacctg tgggaaaaaa tgtacagccg
      661 ctccctccgg gagcggggag agctcgaaga acgggaaaaa tgccttcggg gagaccggga
      721 ggaactgctc cggaagaggg aggatctccg gagaagctgt ttcgcatccg ccgccgccgt
      781 ccgggagatc cggtcgagaa tgggctccct tgaaggggag gaaaaaaccc tttctgcccg
      841 ccttgaggaa atgggggccg aatggggcga aattctctcg aaggaaaacg accttgccgc
      901 gggacttgcc gccgctgttg aggaaaggga caggctccgg agacggatga acgaacttcg
      961 ggcggccatc gaaaaagagc aggagaggaa aaaaaacctc tcagccgccc tctcggccct
     1021 tggtgcggaa aaggagaccc tccggtcgaa aagggaatcc cgggaggcct ttctggccga
     1081 ctgcgacgcc aggatcagga cctcgggaga gattttggcc gctctgcagg aagaatcggc
     1141 tgcccttgaa gaaaaaatag ccggccttga acggagggag gccgaagtgg tggaggctca
     1201 cggcgacgcc ttcgccgcct gcaggaagac ggcttccgcc ctccagcagg cccggaagga
     1261 atccgcttct ctcgaggcgg ccgccgagga cctgaagacg gcagaaagtt catcctaccc
     1321 cgaacccgtc cgcttcctca cctctgccta ccgcctcgga aagctccagg ttccggtgac
     1381 ggtggccgcc gagtcctttt cctgcccgcc gtccgttacc tcggccctgg aggcctacct
     1441 cggagggcgg cagtactgga ttttcgtgaa gactctcgcg gaggcgggag cctgcatcga
     1501 cctcctcaag gagcgccggg ccggacgggc caccttcctg cccctggaaa aaagccgtcc
     1561 ccggtttccc gacaggaggg ccgtccttcc cgcccggggc gtcgtgggat gggccattga
     1621 gatcgtcacg ccctcggagg agtgggaatc ctgtgtttcc catcttctcg gggatctgct
     1681 gctggtggag gagtactccg tgggggctcg gctcgtctca gggggggctt cgtacccgat
     1741 cgcgtccctc gagggggaag tgttcctccc ctcgggcacg gtgagcggcg ggcggacgcg
     1801 ggccgccgcg ggggccattg aacggcgccg ccgcatccgg gaggcggagg agcgcctcgc
     1861 ccgggtcagg caggaagggg ccgccctggc cgccgccctt gaaaaagaag aagccctgga
     1921 acgggcccgg tccgccgaaa aggaagccat ttccctggaa ctgcgggaaa aaagaaagcg
     1981 cctggaggaa aaacggcggg acatggatct cgagcgggca gccggtgagc ggctggaccg
     2041 cgaccggctc caggccctcg acgattttgc ctcctgggag aaacggatgg cggaaatcga
     2101 cggggagatg gaagttctct ccgcctccat gccgtccacc gcagagggcg atgaagaatc
     2161 ctcccttccg gcacggctca ccgaagcgga aaacagctgt gtccttctgg aggagcgcct
     2221 gtccagcgtt tcctccctca ggaaccgggc agcatcggaa ctgga
//
LOCUS       DAOTHD010000329         2260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_56769_length_2260_cov_3.035828, whole genome shotgun sequence.
ACCESSION   DAOTHD010000329 DAOTHD010000000
VERSION     DAOTHD010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56769_length_2260_cov_3.035828"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="P5219_06355"
     CDS             <1..965
                     /locus_tag="P5219_06355"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HRX26412.1"
                     /translation="YILPGDIVLGADSHTCTGGALGALSTGVGSTDLAAVWALGETWL
                     KVPPTVRVDFRGTRPGWVTGKDMILSLIGRISVQGARYMALEFGGEGIENLPMDDRFT
                     IANMAVEAGAKTGVFVPDEKTLEYARSRAAREFTPRYPDSDAPYARRETFDLDGMGPV
                     AAMPHSPDNVKPVRECGSPEIDQVFIGACTNGRFRDIETAAKLMEGKKVAPNVRCIVI
                     PASYEVYNAAMDKGYLRIFTEAGAAVCTPTCGPCLGGHMGILAAGERCVSTSNRNFVG
                     RMGSPKSELILASPLTAAASAVTGRLTDPRDVMPEEFFRNLEGN"
     gene            968..1483
                     /locus_tag="P5219_06360"
     CDS             968..1483
                     /locus_tag="P5219_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRX26413.1"
                     /translation="MTKAFSGKAWKYGDHIDTDVIIPARYLVSSDETVLGKYCMEDID
                     KDFVRNMSKGDMIVAGDNFGCGSSREHAPLAIKGAGCSCVIAASFARIFYRNSINVGL
                     PIFECPEAAASIETGDEVAVDPIAGTIENRTKGMTFRVAPFAPFLQELISAGGLIPYA
                     RRRLEERRKGA"
     gene            1480..>2260
                     /locus_tag="P5219_06365"
     CDS             1480..>2260
                     /locus_tag="P5219_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRX26414.1"
                     /translation="MTGKNYVVARIPGDGIGPEVIGETSRILEAAGRKHGFSLTWADY
                     PFGADHYLKTGEILPASAVEEMGSMDALLLGAVGDPRVKPGILERGILLTIRFHFDQY
                     VNLRPAKSYPRVPLPVAPKGGKDLDTLVVRENTEDFYIGIGSRTEKGTADFSLDTKRG
                     LYSLEGKLRGTFSGGVEGAFQLGVASEPGVRRVVAYGCRAAKARGEDKITLASKSNAL
                     PQIYGFWEDIAKDEAARHGMGLGIVNVDAMCYHLVRTPDLYG"
BASE COUNT          456 a          736 c          707 g          361 t
ORIGIN      
        1 gctatattct ccccggtgat atcgtccttg gggcggacag ccacacgtgc accggcggcg
       61 ccctgggcgc cctctccacc ggtgtgggaa gcacggacct cgctgcagtc tgggccctcg
      121 gggaaacgtg gctcaaggtg cctcccaccg tccgggtcga tttccgggga acgcgccccg
      181 ggtgggtgac cggcaaggat atgatcctct ccctcatcgg gcgcatcagc gtccagggag
      241 caaggtacat ggctctcgag ttcggcggcg agggaattga aaacctgccc atggacgaca
      301 ggttcaccat cgccaacatg gccgtggaag caggcgcaaa aacgggagtc ttcgtccccg
      361 acgagaaaac cctcgagtac gcccggtcga gggcggcacg ggaattcact cccaggtacc
      421 ccgacagcga cgcgccctat gcccgccggg agaccttcga cctcgacggg atgggccccg
      481 tggcggccat gccccactcc cccgacaacg taaaacccgt ccgggagtgc gggtcgcccg
      541 agatcgacca ggtgttcata ggggcctgca ccaacggccg cttccgggat atcgaaaccg
      601 ccgcgaagct gatggagggg aaaaaagtgg cccccaacgt gcgctgcatc gtcatccccg
      661 cctcctacga agtctacaac gccgccatgg acaagggcta tctccgcatc ttcaccgagg
      721 ccggagccgc cgtctgcact cctacatgcg gtccctgcct gggaggacac atgggaatcc
      781 tcgccgcggg agaacggtgc gtgtccacca gcaaccgcaa cttcgtgggc cgaatgggca
      841 gcccgaagag cgagctgatc ctcgccagcc ccctcaccgc ggccgcgagc gccgtcaccg
      901 gcaggctcac cgatccgaga gacgtgatgc ctgaagaatt cttcaggaac ctggagggaa
      961 attaatcatg acgaaggctt tttcagggaa agcgtggaaa tacggcgatc atatcgacac
     1021 tgacgtgatc atccccgccc gctacctcgt ctccagcgac gagacggttc tggggaaata
     1081 ctgcatggag gacattgaca aggactttgt ccgcaatatg tccaaaggcg acatgattgt
     1141 cgccggggat aatttcggct gcggatcctc ccgtgagcac gcgcctctcg ccataaaggg
     1201 cgccgggtgc agctgcgtca tcgctgcctc cttcgcccgg attttctaca gaaactccat
     1261 caacgtgggg cttcccatct tcgagtgccc cgaagccgcc gcttccatcg agaccgggga
     1321 cgaagtggcc gtggacccca tcgccggaac catcgagaac cggacgaagg gaatgacttt
     1381 ccgggtggcc ccctttgccc ccttcctcca ggagctcatc agcgcaggcg gactcatccc
     1441 ctacgcccgc aggcggctcg aagaacggag gaaaggcgca tgaccgggaa gaactacgtg
     1501 gtggccagga ttccggggga cggcatcggg cccgaggtca tcggcgaaac atcccggatc
     1561 ctcgaagcgg cgggccggaa gcacggattc tccctgacct gggctgacta ccccttcggc
     1621 gccgaccatt acctgaagac aggagaaatc ctgcctgcct ccgcagtgga ggagatgggg
     1681 tccatggacg cactgctcct tggtgcggtg ggagatcccc gggtcaagcc gggaatcctc
     1741 gaacggggaa tactcctcac catccggttc cacttcgacc agtacgtgaa cctccggccc
     1801 gcgaagagct atccccgggt tcccctcccc gtggcaccga agggaggaaa ggatctggat
     1861 accctcgtcg tccgggagaa cacggaggat ttctacatcg gcataggcag caggacggaa
     1921 aaggggacgg cggacttcag cctggacacg aaacggggac tctattccct cgaggggaaa
     1981 ctcaggggga ccttctccgg cggcgtggaa ggggccttcc agctcggcgt ggcctccgag
     2041 ccgggcgtcc gcagggtggt cgcctacgga tgccgggcgg cgaaggcccg gggcgaggac
     2101 aaaatcaccc tggcgtcgaa gtcgaacgcc ctcccccaga tctacggctt ctgggaggac
     2161 attgccaaag acgaggcagc ccgccacggc atgggtctcg gcatagtgaa cgtggacgcc
     2221 atgtgctacc acctggtgcg cacccctgac ctctacggcg
//
LOCUS       DAOTHD010000330         2259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_56798_length_2259_cov_3.852995, whole genome shotgun sequence.
ACCESSION   DAOTHD010000330 DAOTHD010000000
VERSION     DAOTHD010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56798_length_2259_cov_3.852995"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="P5219_06370"
     CDS             complement(<1..1138)
                     /locus_tag="P5219_06370"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRX26415.1"
                     /translation="MISLSRGDLYRTLLDLAAIPSVSFSGGENGAADYIYSRLAELDY
                     FQRNPSFLRKLPAEGDPLGRCAVAALVKTSPEMKRTVILTGHFDVVDAEAYGPLKHLA
                     FSPEELTRLVGELEIPDEARADLATGEYLFGRGVSDMKSGIAVEMGLLAEISRTGDFP
                     ANVLFLAVPDEENTSAGMRGAIPWLVKLREEWGIEYSAALNGEPSVGGRERPAGTVYV
                     GTIGKIMPFFLCVGKEAHVGDYFEGVSASLLTACLALVLEGAASTSEEREGKSFPPMA
                     CLRFRDLVKNYSVTLPERAVAYYNLLTVEKTPAGVLGEMKSAAGEALKNALGRISEQK
                     GLIGSRGGSSPAAPRFSPRVMDFAELLTRARNRNPDLEVLTEGFL"
     gene            complement(1142..>2259)
                     /locus_tag="P5219_06375"
     CDS             complement(1142..>2259)
                     /locus_tag="P5219_06375"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRX26416.1"
                     /translation="KVLSENEKDKTITQRTQRRITSLEKTIQHVNSTVAHAFSLFNSY
                     SRLEEEINSRIDAVRIAAQVTRFSKERVMAFLNMSLWSGEGYNVTVSKLVISLFLFGA
                     AFFLSGWLTRLVGRTVLRRFKMDPTAQIATQKILFYIFMVSFILSALDVAGIPLTAFA
                     FLGGALAIGIGFGAQNFFNNLISGFILMFTKPIRMNDTIEIDGLFASVEEIGSRSTHV
                     KTFDNIDVLVPNSYFLNNNIINWTHTDQKIRQRLNVGVAYGSDVRKVEELLYKAAHDH
                     SRILKTPEPFVIFRDFGASSLDFTLFYWIDMTKASTLKVGSDLRFRIVALFEEHGIVI
                     AFPQLDVHLDAESPLQLTMMRGKNRKPAEAEREQTEA"
BASE COUNT          463 a          660 c          645 g          491 t
ORIGIN      
        1 ggaggaaccc ttccgtgagt acctcgagat cgggattcct gttcctggcc ctggtcagga
       61 gttctgcaaa atccatgacc cgggggctaa aacgcggcgc tgccggagaa gaacctcccc
      121 tgctcccgat caggcccttc tgctcgctga tcctgccaag agcatttttc agggcctccc
      181 ctgccgccga cttcatctcc ccgagcaccc cggcgggtgt tttttccacg gtgagcagat
      241 tgtaatacgc caccgcacgc tcgggaaggg tgacggagta attcttcacc aggtccctga
      301 accggaggca ggccatgggc ggaaagcttt ttccttcccg ttcctccgac gtggaggcgg
      361 ctccttcgag gacgagggca aggcaagccg taaggaggga ggcgcttacg ccctcgaaat
      421 aatcgcccac gtgggcctcc ttccccacgc agagaaagaa gggcatgatc ttgccgatgg
      481 tgccgacata caccgttccg gcaggccgtt cccgtcctcc tacggaaggc tctccgttga
      541 gggcggcgga gtattcgatc ccccactctt ccctcagctt cacgagccag gggatcgccc
      601 ccctcatccc cgccgaggtg ttctcctcgt ccggaaccgc caggaagagg acgttggcgg
      661 ggaagtcccc cgttcttgat atttccgcca ggagccccat ctctacggca atcccggact
      721 tcatgtcgga aacgcccctg ccgaacagat attctcccgt tgccaggtcg gcccgggcct
      781 cgtccgggat ctccagctcc cctaccagcc tggtgagctc ttcgggggaa aaggcgagat
      841 gcttcagcgg cccgtaagcc tcggcatcca ccacgtcgaa atgtcccgtc agaatgaccg
      901 ttcgtttcat ttccggtgac gttttcacca gggcggccac ggcacatctg ccgaggggat
      961 ccccttccgc gggcagcttt ctcagaaagg aggggttgcg ctggaaatag tcaagctcgg
     1021 ccaggcgcga ataaatgtaa tcggctgccc cgttttcccc cccggagaag gaaacgctgg
     1081 gaatggccgc caaatcaagg agcgtcctgt acaaatctcc gcgtgaaagt gaaatcatcg
     1141 gttacgcctc cgtctgctct ctttcggctt ccgcgggctt cctgtttttt ccccgcatca
     1201 tcgtcagctg caggggactc tccgcatcaa ggtgaacatc aagctgcggg aaggcaatga
     1261 caatgccgtg ttcttcaaaa agggcgacga tgcggaatct caggtcgctt ccgaccttca
     1321 gcgtggaggc cttcgtcatg tcgatccagt aaaagagggt gaaatcaagg gaactggcgc
     1381 cgaaatccct gaaaatgacg aaaggttccg gcgttttcag aatcctcgag tgatcatgag
     1441 ccgctttgta cagaagctct tccactttgc ggacatccga accgtaggcc acgcccacgt
     1501 tgagtctctg gcggattttc tggtccgtgt gggtccagtt gatgatgttg ttgttcagga
     1561 aatagctgtt cggaaccagc acatctatgt tatcgaaagt cttcacgtgg gtggaccgtg
     1621 aaccgatctc ctccaccgag gcaaagaggc cgtcgatctc gatggtgtcg ttcatgcgga
     1681 tgggcttcgt aaacatgagg atgaagccgc tgatgaggtt gttgaagaaa ttctgtgcgc
     1741 cgaaaccgat gccgatggcc agggcaccgc cgaggaatgc gaaggctgta agcggtatgc
     1801 cggcaacgtc cagggcggag aggatgaagg ataccatgaa gatgtaaaag agaattttct
     1861 gggtcgctat ctgggctgtg gggtccattt tgaagcgcct caggacggtt ctcccgacaa
     1921 gcctggtcag ccagccgctc aggaagaacg ccgccccgaa aagaaagagc gaaatgacca
     1981 gcttcgaaac agtgacgtta tacccttccc cggaccacag ggacatgttc aggaaggcca
     2041 tgacgcgctc cttgctgaac cgggtcacct gggcggctat tctgacggcg tcgatcctgg
     2101 aattgatttc ctcttccagt cgcgaatagc tgttgaacag gctgaacgca tgggctaccg
     2161 tcgaattgac gtgctggatt gtcttctcca gggaagtgat tctcctctgg gtcctctggg
     2221 ttatggtctt gtccttttcg ttctccgaca ataccttct
//
LOCUS       DAOTHD010000331         2259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_56803_length_2259_cov_3.333485, whole genome shotgun sequence.
ACCESSION   DAOTHD010000331 DAOTHD010000000
VERSION     DAOTHD010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56803_length_2259_cov_3.333485"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /gene="guaA"
                     /locus_tag="P5219_06380"
     CDS             complement(<1..782)
                     /gene="guaA"
                     /locus_tag="P5219_06380"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRX26417.1"
                     /translation="MDNIVILDCGSQFTQLIARRVRELKVHSEILPWDVSLGEIESRR
                     PSGVIISGGPMSVTDEDAPAVPEGLFSLGVPVLGVCYGMQLVTSMMGGRVTSGTAREY
                     GRTEISLSGESLLFDGVPSSFEVWMSHGDHIDVLAPGFRLVAKTAKGVPAAMENPEKG
                     IWGLQFHPEVVHTRFGTEILSNFLFRVCSCSPDWDLGNWIDIMVEEIRQKTGNDRVIC
                     GLSGGVDSSVAAVLTARAIGERLDCIFVNNGLLRLGEAEEVL"
     gene            complement(892..1878)
                     /locus_tag="P5219_06385"
     CDS             complement(892..1878)
                     /locus_tag="P5219_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HRX26418.1"
                     /translation="MTDFPAPFLLLPAYKDYLWGGNRLASLFGKMGGPERIAESWELS
                     AHPDGKSVIAGGPFRGQTLSAFLESHPGAAGTAVPPGEPFPLLVKLIDAAENLSVQVH
                     PGDEYARRTEGNRGKTEMWYVLRSDPGAFLYCGFSLPVTRGEVEERIREGTLPEVLRS
                     VPVEPGDVFFIPAGTIHAIGEGIILAEVQESSNITYRVFDYGRAGADGRPRPLHIGKA
                     LDVLRLDEGCPPPPGVEIGFRSVPGGELRLLCSCPSFTCSVLELNGRFSQKMGDEFFL
                     SLLCLDGKARLSSSGGGLGIGTGDSAFVPAGRRDILLEGTGEFLLTFPGRPE"
     gene            complement(1875..>2259)
                     /locus_tag="P5219_06390"
     CDS             complement(1875..>2259)
                     /locus_tag="P5219_06390"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRX26419.1"
                     /translation="AALADKLQGALPAISLCSQQALVTAVANDGDYGLVFAQQVMGYA
                     GPGDVVLGISTSGNARNVKWALMAAKARGAFSMALTGEGGGALRDAADLWIPVPDGRT
                     FRIQELHGAVYHLLCAFAESEMFPE"
BASE COUNT          456 a          735 c          669 g          399 t
ORIGIN      
        1 tccaatactt cctcggcctc gccgagtcgg agcagaccgt tgttcacgaa aatgcagtcc
       61 agtcgttcgc cgatggcccg ggccgtgaga actgccgcca ccgaggagtc cacgccgccc
      121 gacaggccgc agatcacccg gtcgttgccg gtcttctgcc ggatctcctc caccatgatg
      181 tcgatccagt tgcccaggtc ccagtcaggc gagcaggagc agacccggaa gaggaaattg
      241 gaaaggattt cggtgccgaa acgggtgtgg accacttccg ggtggaactg gagtccccag
      301 atgccttttt cgggattctc catggccgcc ggaacgcctt tcgcggtctt cgcgacgagc
      361 cggaagcccg gggcgaggac atcgatgtgg tcaccgtggc tcatccagac ctcgaaagaa
      421 gacggcacgc catcgaaaag aagggattct ccggaaaggg aaatttccgt acggccgtac
      481 tccctcgccg ttcccgatgt gaccctgcct cccatcatgc tggtgacgag ctgcatcccg
      541 taacagacgc ccagcacggg cacccccagg gaaaacagtc cttccggaac ggcgggggcg
      601 tcctcgtcgg tgacgctcat ggggcctccg gagattatga cgccgctcgg cctgcggctt
      661 tcaatctccc cgagggaaac atcccaggga aggatttcgc tgtgcacctt cagttccctg
      721 accctgcggg cgataagctg ggtgaactgg gaaccgcaat caagaatgac aatgttgtcc
      781 aaaaatccat tcctcccgaa tcaatggggt gtatagcaca ggtatcttat cagaaagccc
      841 ttcttcgttc cacagaagag aacattgacc tgagtctccg ggcaagtaac attactccgg
      901 tctgccgggg aatgtcagca gaaactctcc cgttccttcc aggaggatgt ctctccgtcc
      961 ggcggggacg aaggcgctgt ccccagtgcc gatcccgagt ccgcctccgg acgatgagag
     1021 acgggctttt ccgtcgagac agagcaggga aaggaagaac tcgtccccca ttttttgcga
     1081 aaaacgcccg ttcagttcca gcacactgca ggtgaaggat gggcaggagc acaggagacg
     1141 aagctcccct ccgggaacgg aacggaaccc tatttccacg ccaggaggcg gagggcaacc
     1201 ttcatccagg cggaggacat ccagggcctt cccgatgtgg aggggcctgg gccgtccgtc
     1261 cgcaccggcc ctgccgtaat cgaagacccg gtacgtgatg ttggagctct cctggacctc
     1321 cgccaggatg ataccttccc cgatggcgtg gatcgttcca gcagggatga agaaaacgtc
     1381 tcccggctcc acggggacgg aacggagaac ctccggcaga gtgccctccc ggatccgctc
     1441 ttccacttcc cccctcgtca ccggcaggga aaaaccgcag taaagaaaag caccggggtc
     1501 gctccggagg acgtaccaca tctccgtctt gccccggttc ccttccgtcc ggcgggcgta
     1561 ttcgtcaccg ggatgaacct ggaccgaaag gttttcggcg gcgtcgatga gcttcacgag
     1621 cagcgggaat ggttctcccg ggggaactgc cgtccctgcc gctccgggat ggctttccag
     1681 aaaggccgac agggtctgcc ccctgaacgg cccgccggcg atcaccgatt tcccgtcggg
     1741 atgggccgac agttcccagc tctcggcgat ccgttccggc ccacccatct taccgaagag
     1801 ggaggccagc ctgttccctc cccacaggta gtccttgtat gccggcagca gcagaaaagg
     1861 agccgggaag tccgtcattc cggaaacatc tccgattcag cgaaggcgca gagcaaatga
     1921 tagacggccc catgaagctc ctggatcctg aatgtccgtc cgtccgggac gggaatccac
     1981 aggtccgctg catccctcag cgcccctccc ccttcgccgg taagggccat ggaaaaggcg
     2041 cccctcgcct tcgccgccat gagggcccat ttcacattcc gggcgttccc cgacgtggaa
     2101 atgcccagaa ccacgtctcc cgggccggcg taccccatga cctgctgggc aaacacgagg
     2161 ccgtaatcgc cgtcgttggc caccgccgtc acgagggcct gctggctgca gagggatatg
     2221 gcgggcaggg ctccctggag cttatccgcc agagccgcg
//
LOCUS       DAOTHD010000332         2259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_56816_length_2259_cov_2.645644, whole genome shotgun sequence.
ACCESSION   DAOTHD010000332 DAOTHD010000000
VERSION     DAOTHD010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56816_length_2259_cov_2.645644"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="P5219_06395"
     CDS             complement(<1..264)
                     /locus_tag="P5219_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HRX26420.1"
                     /translation="MKTYAIGLVPGPVSVPEEFRRAYLENYGSADLEEDFFLLCEETE
                     GLLRQLLKTQNPVTVQSGEAMSVLWGALKSCLLPGDRLLAVSNG"
     gene            complement(371..1834)
                     /locus_tag="P5219_06400"
     CDS             complement(371..1834)
                     /locus_tag="P5219_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRX26421.1"
                     /translation="MEEATVRQIHEAMKRGETSCRAITEEYIRRIEAYDRKGPSLHAV
                     ILVNPKALEEADELDRIFREKGPVGPLHGIPVLLKDNVETADMVTTSGSLSLEHYLPD
                     DDAFITRKFREAGALILAKANMHEFAVWGESVSSILGQVINPYDLTRTPGGSSGGTGA
                     GMAANFAAVGIGTDTINSIRSPSSACSCVGIRPTIGLVSRDGISPYSLDQDTAGPICR
                     TVEDAVRVLDVIAGYDPADPETAWAVGNAPESYMDYLCPDGLKGKRIGVLRSLFGDKP
                     EHEDVNAAMKRSLDAMKEGGAELVEVSEPIDTNYLASKVSVHLHTLKDDLEGYLKSLG
                     DKCEYHTVEALIDSGKIHPGIVENMKTAAGLSKDSDEYRSRTLKRLALKTQVMKLMAD
                     LKLDALVYPHQKRLAVPVGESQVERNGVLGSATGFPSIAVPAGFSRPTESAPEGVPIG
                     LEILGRPWSEGILISMAYAFEQRTGFRKPPFSTPPLS"
     gene            complement(1915..>2259)
                     /locus_tag="P5219_06405"
     CDS             complement(1915..>2259)
                     /locus_tag="P5219_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HRX26422.1"
                     /translation="FSAAIVSISTLNTFYLWTFISSSIVNLFVPSGGGQWAVQGPIAM
                     KSAEMMGASYIKSALAVAYGNTWTNMLQPFWAIALLGITGLKARDVIGYATAVMILSF
                     VVFAIPLLFLPV"
BASE COUNT          387 a          687 c          737 g          448 t
ORIGIN      
        1 tccgttggaa acggcaagaa gacggtctcc gggcaggagg cagcttttca gggcccccca
       61 gagaacggac atggcctcgc cgctctggac ggttaccgga ttttgagtct tcaggagctg
      121 ccgaagcagg ccttcggtct cttcacagag gagaaaaaaa tcctcctcga ggtcggcgct
      181 gccgtaattc tcgagatagg ctctccggaa ttcctcaggc accgaaacgg ggcccggtac
      241 gagccctatg gcataggttt tcatccgatt ccctccctgc aggcggggag ccgcatccgg
      301 ccgcggccgc ctgaaaaagt gaaagggggg agaccttaag gtctcccccc ggtccgtccc
      361 ctattgtacc tcaggaaagc ggtggagtgc tgaagggcgg tttccggaaa ccggtacgct
      421 gttcgaaagc gtaggccatg gagatgagga ttccttcgct ccaggggcgg cccaggattt
      481 ccagcccgat ggggactcct tcgggagccg actccgtggg cctggagaat cccgccggca
      541 cggcgatgga cggaaagccc gtggctgagc cgaggactcc gttgcgctcc acctgggatt
      601 cgcccacggg tacggcgagc cgcttctggt ggggatagac gagggcatcc agcttcaggt
      661 ccgccatgag cttcatcacc tgggtcttca gggcgaggcg cttcagtgtc cgggaccggt
      721 attcgtcgct gtccttgctc agtcccgcag cggtcttcat gttctccacg atgccggggt
      781 ggatcttgcc ggagtcgatg agggcctcca ccgtgtggta ctcgcacttg tcgccgagac
      841 tcttcaggta cccctcgagg tcgtccttga gggtgtgaag atggacgctc accttcgacg
      901 ccaggtagtt ggtgtctatg ggctcggata cctccacaag ctcggccccg ccctccttca
      961 tggcgtcgag gctccgcttc atggcggcgt tcacgtcctc gtgctccggc ttgtctccga
     1021 agaggctccg gaggacgccg atgcgctttc ccttcagccc gtccgggcag agatagtcca
     1081 tgtagctttc gggggcgttc ccaacggccc atgccgtctc cgggtccgcc ggatcatacc
     1141 cggcgatgac gtcgagcacc cggacggcgt cctccacggt gcggcagatg gggcccgccg
     1201 tgtcctggtc cagcgagtag ggggagatgc cgtcccggct cacgaggccg atggtgggcc
     1261 ggatgcccac gcagctgcag gccgaggacg gggaccgtat ggagttgatg gtgtcggtgc
     1321 cgatgcccac ggctgcgaaa ttggccgcca tgccggcgcc ggtgccgccg ctcgagccac
     1381 ccggcgtccg ggtgaggtcg taggggttga tcacctgccc gaggatggaa ctcacgctct
     1441 caccccacac ggcgaactcg tgcatgttcg ccttggcgag aatgagggct cccgcctccc
     1501 ggaacttcct cgtgatgaag gcgtcgtcgt cgggaaggta gtgttcaagc gagagggaac
     1561 cgctggtggt caccatgtcc gccgtctcca cgttatcctt gagcagcacg ggaatgccgt
     1621 gaaggggtcc cacggggccc ttttcccgga agatccggtc gagctcgtcc gcttcctcca
     1681 gcgcctttgg gttgaccagg atgacggcgt ggagggaagg ccctttcctg tcgtaggctt
     1741 caatgcggcg gatatactct tccgtgatgg ccctgcagga tgtctcgccg cgcttcatgg
     1801 cctcatggat ctgccgcacg gtcgcttctt ccacgcgaaa acccttttcg gacaatcgtt
     1861 ccacttcctt tcgttcagaa tattccggtg aggctgtcgg ctccgtcctt tatcctacac
     1921 cggaaggaac aggagaggaa tcgcgaagac gacgaagctc aggatcatca cggcggtggc
     1981 gtagccgatc acgtcccggg ccttcaatcc cgtgatgccg agaagcgcta tggcccagaa
     2041 gggctgcagc atgttcgtcc aggtattgcc gtacgccacg gcaagggccg acttgatgta
     2101 gctggcgccc atcatttccg ctgatttcat ggcgatgggg ccctggacgg cccactggcc
     2161 gccgccgctg gggacaaaga ggttgacgat gctggagctg atgaaggtcc agagatagaa
     2221 ggtgttcagc gtgctgatgg agacgatggc cgcggagaa
//
LOCUS       DAOTHD010000333         2258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_56852_length_2258_cov_2.665002, whole genome shotgun sequence.
ACCESSION   DAOTHD010000333 DAOTHD010000000
VERSION     DAOTHD010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56852_length_2258_cov_2.665002"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="P5219_06410"
     CDS             <1..928
                     /locus_tag="P5219_06410"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HRX26423.1"
                     /translation="AWDYPSAAGTTLVDRPALEWPRDLFAAEGVDPDRFPPLRWPLSV
                     LGRTTAGAARETGLPEGIPVAVGACDINAEAAAATAFFPGDCVVVFGSTVSMLLTTDT
                     PLEVPGFVSGMSLLEGTYRIGAATASGSRFLQWMNRTFRSGDPEREQSPTGILILPWL
                     DGARTPFHNPDGKMTFTGIDGTATPERLIRAGREALGYELSFLLSKIGEFRPVPDVLD
                     VSGGLCNDRILMGIISSITGRKLRLHRDVDASYGDALLAAAASGFLPPEELKDMRGGG
                     TMVFPDRTLHEKFLPWNRRFRDLAENGGVTRP"
     gene            complement(925..2013)
                     /locus_tag="P5219_06415"
     CDS             complement(925..2013)
                     /locus_tag="P5219_06415"
                     /inference="COORDINATES: protein motif:HMM:NF016999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HRX26424.1"
                     /translation="MTGLLLVFAAGISGYLFFRHFRLPVPGVLGSLCFAAALNLAGFY
                     PEFPLRYLVWFSNIVIGTYVGQKVTRTSVRFLRDLPVPALTISAGMLAISLTGGTLLY
                     FMSDLSPVTAFLGSTAGGISEMALLSMSLGADVASVTLLQVFRLLAAILATPVFCRKW
                     TAWHEGRTQGALSRVCPLPAEIGDIGDDDLPPSPGRNRPSARGYAILAAAALAGGFTG
                     YRLHLPVGILTGAMFTVSAMNLAGKEQPPLPGGLRTAAQIGIGIIIAANITTDTLRQF
                     TTMAGPVILITLAMLAGSLSLGFLLHRMTGWNYPTCLLSTSLGGLSQMSIISEEMGAD
                     PLKVSILQTVRLVTILMVFPLLMTFLFG"
     gene            complement(2038..>2258)
                     /locus_tag="P5219_06420"
     CDS             complement(2038..>2258)
                     /locus_tag="P5219_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRX26425.1"
                     /translation="WTARTALSLGLTGWVRNLPDGSVEVQAQGERESLEKMAVWLRRG
                     PSSARVTRLTIIGKPPQRGEESFVIRHF"
BASE COUNT          441 a          683 c          753 g          381 t
ORIGIN      
        1 ggcctgggat tacccttccg ccgccgggac gaccctggtg gaccggcccg ctctggagtg
       61 gccccgggac ctcttcgccg ccgaaggagt cgatccggac cgtttccccc ctctccgctg
      121 gcctctttcc gtcctgggaa ggacaacagc cggtgcggcc cgggaaaccg gactccccga
      181 aggcattccc gtggcggtcg gggcctgtga catcaacgcg gaggcggcag cggccacggc
      241 ctttttcccg ggagactgcg tggtggtctt cggctccacg gtgagcatgc tcctgaccac
      301 ggataccccc cttgaggttc cgggtttcgt ctcgggcatg tcccttctgg agggaaccta
      361 tcgcatcggg gctgccacgg cttcgggatc ccggttcctc cagtggatga accggacgtt
      421 ccgttccggg gatccggagc gggagcagtc tcccaccggc attctcatcc tcccgtggct
      481 cgacggggcg agaacccctt tccacaaccc cgatgggaag atgaccttca ccggcataga
      541 cggtaccgcg accccggagc gcctgatccg ggcaggaagg gaagctctcg gctacgagct
      601 ttccttcctg ctgtcgaaga tcggggaatt ccggcccgtt ccggacgtcc ttgacgtttc
      661 cggggggctt tgcaacgaca ggattctcat ggggatcatc agttccataa cgggaagaaa
      721 actccgtctt caccgggatg tggacgcttc ctacggagat gccctcctgg ccgctgccgc
      781 atcgggcttc cttccgccgg aagagctgaa agacatgaga ggcggaggaa ccatggtctt
      841 tcccgaccgg accctccacg aaaaattcct gccctggaac cgccgcttca gggacctggc
      901 cgaaaacggg ggagtgaccc gcccctagcc gaacagaaag gtcatgagaa ggggaaatac
      961 catcagaatc gtcacgaggc ggactgtctg gagaatgctc accttcaggg ggtccgcccc
     1021 catttcttcc gagatgatgg acatctggga gagccctccc agtgaagtgg acagcaggca
     1081 ggtggggtag ttccatcccg tcatcctgtg gagcaggaag cccagggaga gactgcccgc
     1141 cagcatggcg agggtgatga gaatcaccgg accggccatg gtggtgaact gtctcagggt
     1201 gtccgtggtg atgttggcgg cgatgatgat cccgataccg atctgggcag cggtcctcag
     1261 acctcccggc aggggaggct gttccttccc ggccaggttc atggcggaca cggtgaacat
     1321 ggcccctgtc aggataccga ccggaaggtg gaggcggtac cccgtgaagc ctccggcgag
     1381 agctgcggcg gccaggatgg catacccccg ggccgaaggc ctgtttctcc cgggggaggg
     1441 aggaaggtcg tcatcgccta tatccccgat ttccgcgggg agagggcaga cccgggaaag
     1501 ggcgccctgc gtcctgcctt cgtgccaggc ggtccatttc cggcagaaga cgggggtggc
     1561 cagaatggcg gcgagaagcc ggaacacctg cagtagggta acgcttgcca cgtccgcgcc
     1621 gagggacatg gaaagaagag ccatctcgga aatgcccccg gcggtggaac ccaggaatgc
     1681 cgtgacgggg gacaggtcgc tcatgaagta gagcagtgtg cctcccgtaa gggaaatggc
     1741 cagcattccc gccgaaatcg taagggcggg gaccgggagg tcccgcagga agcggaccga
     1801 ggtgcgggtc accttctgtc caacgtaggt gccgatgaca atgttgctga accacaccag
     1861 gtaccggagg gggaactcgg gataaaaacc tgccaggttc agggcggcgg cgaaacaaag
     1921 agacccaagc acgcccggga cgggcagcct gaagtgccgg aaaaacagat agccgctgat
     1981 tcctgcggcg aagacgagga gaagacctgt catgggggat aacctccgga taaaatgtca
     2041 gaaatggcgt atgacgaagg actcctcccc gcgttggggg ggcttcccaa tgatcgtcaa
     2101 tcgtgtgacc cgggcggagg aggggccccg ccgcagccag accgccattt tctccaggga
     2161 ttcccgttct ccctgggcct ggacttccac ggaaccgtcg ggaaggttgc ggacccatcc
     2221 cgtgagcccg agggaaaggg cagtcctggc tgtccagt
//
LOCUS       DAOTHD010000334         2254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_57002_length_2254_cov_2.695316, whole genome shotgun sequence.
ACCESSION   DAOTHD010000334 DAOTHD010000000
VERSION     DAOTHD010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57002_length_2254_cov_2.695316"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            487..1794
                     /locus_tag="P5219_06425"
     CDS             487..1794
                     /locus_tag="P5219_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX26426.1"
                     /translation="MYRTAMESLKQWKSRENKRPLLIRGARQVGKTWLMKEFGRKEYA
                     ETVYINFDGNRQMKELFSPDFDVDRILIGLELYAGKRILPGETLLVFDEVQEVPDALA
                     SLKYFSENAPRHHILCAGSLLGVALHPETSFPVGKVEFLDLYPLSYTEFLRALGKERF
                     ADVLKKGDFALASTFAQTYTDLLKYYYFVGGMPEAVSHFSQTMDFSGTREIQQRILSA
                     YEQDFSKHAPNDTVPRIRMLWNSIPSQLARENRKFMYGLIREGARAKEYEMAMLWLAD
                     CGLVHKVHRVSGASLPLKAYTDLRAFKLFFSDVGLLSCMAGLSQGTLVDGNVLFREFK
                     GALTEQYVLQQLKAAEGMDVFYWSSEKGTAEVDFLFEAGGDIVPLEVKAEVNLQAKSL
                     KAFREKYRPKISVRTSMAPWKKEDWLVNLPLYAVQYIREAIGG"
BASE COUNT          494 a          602 c          654 g          504 t
ORIGIN      
        1 gtcccggatc gtctcggcca gccagtccag aaaggcttcg tattcctcgt accgttttct
       61 gccgtagagg gtgcggccga gatgccagtc cgacgtgtgg aggattttca tttccggtgt
      121 gtgagagccc cttcgcgggc tcgaaaaggt atgtccccgg ggcaaagggg ggcagccggg
      181 gtccggaaga gactgtcggg cgagggtttc attcttttca tggggacacc tccggtaagg
      241 ttccccccat tgtcggccta ttccaccggg ccgtcaattc ccggtggccc ggtggcccgg
      301 catgattgcc gccctgaaga gcagggcgac gaagggcctc attggcagtc acttctcctc
      361 ttcaagtgca tctttttacc catttcagcc tccatcacga aaagtcatta gtaataatgt
      421 gtttaaaaaa cagaaagtcc attgtttatt tgttataatg cctcctgaat aggcaggtga
      481 tgctgaatgt acagaactgc aatggaatcg ctgaagcagt ggaaaagcag ggagaacaaa
      541 aggccgcttc ttatccgcgg agcccggcag gtgggaaaga cctggctgat gaaggagttc
      601 ggacggaaag agtacgccga aaccgtctat atcaattttg acggcaaccg gcagatgaag
      661 gagcttttct cccctgattt cgacgtggac cgtatcctca tcggccttga actctatgcg
      721 gggaaaagaa ttcttcccgg tgaaaccctt ctcgtcttcg acgaagtcca ggaagtcccc
      781 gacgccctcg cttcactgaa gtatttcagc gaaaacgccc cccggcatca catcctgtgc
      841 gccggctctc ttctcggtgt ggcccttcac ccggaaacct cctttcccgt ggggaaagtg
      901 gagttcctcg acctgtaccc cctctcttac accgagtttc ttcgtgccct tggaaaagag
      961 cgatttgcgg acgtactgaa aaaaggggat ttcgcccttg cctccacgtt cgctcaaacc
     1021 tacaccgatc tgctgaagta ttactacttc gtagggggta tgcctgaagc ggtgtctcac
     1081 ttctcccaga ccatggattt ctccgggaca agggagatcc aacaacggat cctgtccgcc
     1141 tatgagcagg acttttcaaa gcacgcgccc aatgataccg ttcccaggat cagaatgctc
     1201 tggaacagca tcccctctca gctcgccagg gagaaccgga agttcatgta cggattgatc
     1261 cgcgagggag cccgcgcgaa agaatacgaa atggccatgc tctggcttgc cgactgcggg
     1321 cttgtccaca aggtccaccg ggtgagcggc gcgtcactcc cgctgaaagc ctataccgac
     1381 ctccgggcgt ttaagctctt cttttccgat gtagggcttc tatcctgcat ggcggggctt
     1441 tcacagggca cccttgtgga tggaaacgtt cttttccggg agttcaaggg agcgctgacg
     1501 gaacaatatg tcctccagca gctgaaagcc gctgaaggta tggacgtatt ctactggtca
     1561 agcgaaaagg gaaccgcgga agtggacttt cttttcgaag ccggcgggga tatcgttccc
     1621 ctggaagtga aggccgaggt gaaccttcag gcaaaaagcc tgaaggcttt ccgtgagaag
     1681 tacaggccga aaatatcagt gcggacatcc atggcgccat ggaaaaagga ggactggctc
     1741 gtgaacctgc cgttgtatgc ggtgcaatac atcagggaag ctatcggggg ataagttggt
     1801 tgtcgatccg agcgagcgca gcgatcgcgt ccccggaggg ggcgaggaac ctcgcctttg
     1861 gttccggact gcctcaagaa cgagatccct cggccttgga actggccttg gtatgacagc
     1921 gctctgtcgt cctgaggagc gaagcgacaa gagagtcgtt ttctattgat tcgctttcta
     1981 ttgattgtca tcctgaggag cagagcgacg aaggatctgg tgttgcccct gcttgtcatc
     2041 ccgaatgagc gaagcgatcg cggtcgcgca gcatccagag tgaagcggag ggagcatccc
     2101 cggtggggaa ggataggtgt tgcctttcct ttgaggggca taaaagcgag atccttcggg
     2161 cgtgatgctg ccctcaggat gacaattgcc ttgtcgtctt gagcgaagcg atcgcggtcg
     2221 cgcagcatcc agagtgaagc ggagggagca tccc
//
LOCUS       DAOTHD010000335         2243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_57360_length_2243_cov_2.659049, whole genome shotgun sequence.
ACCESSION   DAOTHD010000335 DAOTHD010000000
VERSION     DAOTHD010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57360_length_2243_cov_2.659049"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="P5219_06430"
     CDS             complement(<1..400)
                     /locus_tag="P5219_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26427.1"
                     /translation="MRTERWKAPAVFLFLCFFLSGAVLSPLAAEGLDVIVSGSWRFPL
                     RDSFWSSPGPSVQSMPGSAALSVVNAGNTREQWGIEVKIQGSPLPEGVKLWVRRSGSG
                     HGAGWIRNGLGFVPVGESPVLLFSGTGDRMQ"
     gene            complement(530..1060)
                     /locus_tag="P5219_06435"
     CDS             complement(530..1060)
                     /locus_tag="P5219_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26428.1"
                     /translation="MKKAILVLLAVLFVVGVAGAVLAEEVDLLFCPPSCDPLPNSDSL
                     NIFWKVQPMSTIYIFDDELKICTYIGAPGSGNFHKGFKYMAAATGVGLRKIVGSISPA
                     LPTGVSMWGLLNRPDGIGASTGPQVLGTADVDLVKDLEKLYGAYGTGEVSLFADNTAA
                     GGNGMVVLKLTILDQI"
     gene            complement(1765..>2243)
                     /locus_tag="P5219_06440"
     CDS             complement(1765..>2243)
                     /locus_tag="P5219_06440"
                     /inference="COORDINATES: protein motif:HMM:NF019998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HRX26429.1"
                     /translation="EALTMSDRIMVMNRGKVEQIGAPLDIYSDPSSLFVADFIGQANI
                     IPGKVLSAEGGAVSVSLPDISVSVRAVKDASFVPGDEAAGVVRPENLLPSSGDGMKGT
                     ILTATFLGGRMEYEVALESGRTVLSFDPFLPGKRMWAEGEAVSLLFDPAVAVALKA"
BASE COUNT          516 a          686 c          559 g          482 t
ORIGIN      
        1 cctgcatcct gtcacctgtt ccggaaaaaa gaagcaccgg gctctcaccg acgggtacaa
       61 accccagtcc gttgcggatc cagcctgccc catgcccgct gccgctccgg cgcacccaga
      121 gcttcactcc ctccggaaga ggtgaaccct gtattttgac ctcaattccc cactgttcac
      181 gggtatttcc cgcattgacc acgctgagag cagcggatcc cggcatgctc tgcaccgagg
      241 gaccgggaga ggaccagaaa gaatccctga gaggaaatct ccagcttccc gaaacgatga
      301 cgtcaagacc ttccgcagcc aggggagaca aaaccgcccc ggacaaaaag aagcacagga
      361 aaagaaaaac tgcgggtgcc ttccaacgtt ccgttctcat gaacatgtcc cccctcgatg
      421 gtgccgtttt gcttgtatag aaaagccggc ggggccttcc aagacacttc attcagttct
      481 tccggccgat gaagcaaacg acgagtacct gtctgtccgc ttccctgcac taaatctggt
      541 cgagaatggt gagcttcagg acaaccattc cgttgccgcc tgcggcggtg ttgtcggcga
      601 aaaggctgac ctcacccgtc ccgtaggcac catacagttt ctcgagatcc ttcacgaggt
      661 ccacgtccgc agtcccgagc acctggggtc ccgtggaggc tccgatgccg tcaggcctgt
      721 tcagcaggcc ccacatggac acgcccgtgg gaagggcagg cgaaatggac ccgacgatct
      781 tccggagccc tacacctgtc gccgcggcca tgtacttgaa acccttatgg aagttgcctg
      841 acccgggagc gccgatatag gtgcagatct tcagttcgtc gtcaaaaata tagatcgttg
      901 acatcggctg gactttccag aagatgttca acgaatcgga attgggaagg ggatcgcagg
      961 acggaggaca aaatagcaaa tccacttcct ccgccaggac agcccctgcc acacctacca
     1021 caaacagcac cgcgagaagc accagtatcg ctttcttcat ttcactcacc tcttctttct
     1081 cggtaaattg ccccaaagaa ggccccgccg gccttcggac acaaaggacc acctgtctgc
     1141 ggagacaatt tcctgtccgc gcagcaaacg ccgaaaatac atttcagacc ctgaaatgca
     1201 gaaccgctgc gaccgggaaa gaattacgga aatgagagaa cggaaaactg aagggattat
     1261 ggaagaccgg gataaagcct ggataaatgg aacgaccatc aaaaagaaaa aactccatgc
     1321 cccacgatgt gcctttacgt cgcatgacct gccccacgca ttgccactgt attgcctttt
     1381 gttttgaccc ttgttttgca ccctgcattg ccccatgcct tgcaggttgc tttacccttt
     1441 gccttgtgcc ttgcattgca tactgccttt cctgcttcgc ctctttgcac atcaaaaatc
     1501 ttcttttctt ttgcgctact atttatttca gctcaatttt aaccattgca tcttctttgt
     1561 caatcaccct gtcattgtat tatgtatttt cgtatttcat gtattcactc accccttttg
     1621 catggttctg atccggcagt atgcaggatg aacgaagaag aatcagcaac cccgaagggg
     1681 gacaaaacat tgtttccggc agatgcatga cgaagggagc cgaagaaaaa cattctccgg
     1741 ctcccttcgt cctcccgcta aatatcaggc tttcagcgcc accgccactg ccgggtcgaa
     1801 caagaggctg accgcctctc cctcggccca catccttttc ccgggaagga aggggtcgaa
     1861 ggagagcacc gtccggccgc tttccagggc gacttcgtac tccatgcgcc cgccgaggaa
     1921 ggtcgcggtg agaattgtcc ccttcatccc gtctcctgag gagggcagga ggttttccgg
     1981 ccggacgacc cccgccgctt cgtctccggg tacgaaggaa gcgtctttaa cggcccgtac
     2041 ggacacgctg atatccggaa gggagacgga gactgcccct ccctccgcgg agaggacttt
     2101 gcccggtatg atgttcgcct ggccgataaa atcggccacg aaaagggagc tcgggtcgct
     2161 gtaaatatcg aggggggccc cgatctgttc cacctttccc ctgttcatca ccatgatccg
     2221 gtcggacatg gtgagggcct ccc
//
LOCUS       DAOTHD010000336         2240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_57467_length_2240_cov_1.984439, whole genome shotgun sequence.
ACCESSION   DAOTHD010000336 DAOTHD010000000
VERSION     DAOTHD010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57467_length_2240_cov_1.984439"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="P5219_06445"
     CDS             complement(<1..764)
                     /locus_tag="P5219_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HRX26430.1"
                     /translation="MNLKSLSIKSIGVLLFIVLIVSSCTVVGYFIFSGWLSSTAGTAA
                     FLADELNGRISGRIGEHMNTPAHLVEVYRDQIEKGVIDMGNETQRERFFTSVLQTHSE
                     GLYSFSFGSEDGEYYGARRNSAGGIEIMRNDRSTGGASWYFTLGEHFTAGKLAVKAGP
                     FDPRTRDWYRTAKDLGKTVFSPVYRHFIMADLAVSAAAPVRESSGKLRGVLGAHVTLS
                     RINSSLEEIAGEGNGTAAIVERKTGALIGNSLGMAN"
     gene            1050..1376
                     /locus_tag="P5219_06450"
     CDS             1050..1376
                     /locus_tag="P5219_06450"
                     /inference="COORDINATES: protein motif:HMM:NF025475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HRX26431.1"
                     /translation="MQTLVDFLAFRHFISPAVLPVCYVLGAAGIPFVVWLAVQRMVPF
                     LGKQAAGPYRSGAEDAPAPRYRGRILFWCTLLFVVAEILWRILFEYLAAFLQMRDALL
                     FLVNGH"
     gene            1435..2169
                     /locus_tag="P5219_06455"
     CDS             1435..2169
                     /locus_tag="P5219_06455"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRX26432.1"
                     /translation="MNSKKKGGESIPGTGSVFEKQALHWGILALLLLALYGVSGMEMI
                     RQGSLWGLGSVQWLWTAAGLAAVHQVYVWFCWRVELHLQFITRYLGKRGFSLYAFGFA
                     ILGILRAAAVFFLAAANRDTLPVHPAILKSLAVISAVPSIYLMYSVARYFTFRRALGG
                     DHFFESYRNAPLVRKGIFRFTPNGMYTYGFLILWSIALWFGSVAALSAAAFNHAYIWV
                     HYFCTELPDMKRIYRSEEKNDLLSGG"
BASE COUNT          448 a          616 c          634 g          542 t
ORIGIN      
        1 aaattcgcca tgcccaggga atttccgata agggcccccg tctttctctc cacgattgcc
       61 gccgtgccgt tcccttcccc ggcgatttcc tccagggagg agttgattcg ggacagggtg
      121 acgtgggcgc cgaggacccc ccgcagtttc ccgctgcttt ccctgaccgg agcggcggcg
      181 gagacggcaa ggtctgccat gatgaagtgt ctgtagacgg gggaaaaaac agtttttcca
      241 aggtctttgg cggtcctgta ccagtccctg gtcctcgggt cgaagggtcc ggccttcaca
      301 gcgagcttac cggcggtgaa atgttccccc agggtgaaat accacgaagc acctccggtg
      361 gaccggtcgt tcctcatgat ctcgattccg ccggcgctgt ttctgcgggc gccgtaatac
      421 tcgccgtcct ctgaaccgaa actgaagctg tacagcccct cgctgtgcgt ctggaggacg
      481 gaagtgaaaa aacgctcccg ctgggtttca ttccccatgt cgatgacgcc cttttcgatc
      541 tggtccctgt acacctccac gagatgggcg ggggtattca tgtgttcacc gatcctgccg
      601 gatatgcgcc cgttcagctc atcggccagg aatgccgcgg tcccggccgt tgacgacagc
      661 caccccgaga agatgaaata tcccacgact gtgcaggagg acacgataag aacgatgaaa
      721 agaagcacgc cgatgctttt gatggagaga ctttttaagt tcatttccac ctctcgtttt
      781 ctccggaacg aggaagactg aaggtacggg aaacatccgc acaagcgcgg gaacccttct
      841 gtttcgtcct gcacggaaag agagcgaggc tcatccgttc tttgccccac tgatgtactg
      901 aaaactgatt ttacagcctt cagggtattt tgtctctgtt ttgtcgtttt tccttcattg
      961 ttcttgtttt tctgcagaac ctcttgcgcc gggaggaaga gtggggtagg gtatcggaag
     1021 gcaagccccc ggaaggagat gtcatatccg tgcagaccct tgtcgatttt ctcgccttca
     1081 ggcatttcat cagcccggcg gttctgcccg tctgttatgt cctgggagcg gccggaattc
     1141 cctttgtggt ttggctggcg gtacagcgga tggtgccctt cctgggaaaa caggcggcgg
     1201 ggccgtaccg ttcaggggcg gaggacgctc ccgcccccag gtacagggga cgaatcctct
     1261 tctggtgtac cctcctgttc gtggttgcgg aaatcctgtg gcgcattctg ttcgaatacc
     1321 tggctgcttt ccttcagatg cgtgatgccc ttcttttcct ggtaaacggt cattaaatgc
     1381 ctggagagcg gagaagcaaa aacttttttt ccgacagaaa aagagggaag ataaatgaac
     1441 tcgaaaaaga aaggcggaga atctatcccg ggaacaggat ctgtctttga aaaacaggcg
     1501 cttcattggg ggatccttgc cctgctcctt cttgccctgt atggggtgag cgggatggag
     1561 atgatccggc aaggttcgct gtggggactg ggctccgtcc aatggctgtg gactgccgca
     1621 ggacttgccg cagtccacca ggtctacgtg tggttttgct ggcgtgtcga gctccacctg
     1681 cagttcataa cccggtactt ggggaagcgc ggattctctc tctacgcttt cggttttgcc
     1741 attctcggga tcctgagggc ggcagccgtg ttctttctcg ctgcagcaaa cagggacact
     1801 cttccggtgc atcccgcgat cctgaaaagc ctcgctgtaa tctcggcggt gccttccatc
     1861 tacctcatgt attccgttgc aaggtatttt accttcaggc gtgccctggg aggggatcat
     1921 ttctttgaat cgtaccggaa cgctcccctc gtccggaagg gaattttcag gttcacgcca
     1981 aacggcatgt acacctacgg ctttcttatc ctctggtcaa tcgccctgtg gttcggttcc
     2041 gttgcggcac tgagcgccgc agcattcaac catgcctata tctgggtgca ctacttctgc
     2101 actgaacttc ccgacatgaa gcggatctac cggtcggaag aaaaaaatga tctgctttca
     2161 ggcgggtagg tgaaaagcat ccgccgggga aaaacctgaa aagagaaatg agaaaagggg
     2221 tgaaggccga aacctccacc
//
LOCUS       DAOTHD010000337         2238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_57530_length_2238_cov_3.841044, whole genome shotgun sequence.
ACCESSION   DAOTHD010000337 DAOTHD010000000
VERSION     DAOTHD010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57530_length_2238_cov_3.841044"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="P5219_06460"
     CDS             complement(<1..261)
                     /locus_tag="P5219_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRX26433.1"
                     /translation="MAVTLELLNVSKIFRKDRELVRAVDNVSMTVAPGEMVTFLGPSG
                     CGKTTTLRMVAGFETPSGGTITIDGQDVTNVPVNRRDIGFVFQ"
     gene            complement(261..1958)
                     /locus_tag="P5219_06465"
     CDS             complement(261..1958)
                     /locus_tag="P5219_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRX26434.1"
                     /translation="MNRPSALSAPEKVSPLKRDPALLPALAAIWISLAVFVLYPVLRL
                     FLMTFMVEGQFSFANLGAVLDSWYDRQAFLNSLWLAGAVSFAGTVLGYIYAFAVTRIP
                     LPSWLKWFLGAVTTLPLISPPFTSSIALTLSLGPNGILLKFFGIENFSFYGFWGTWMS
                     ETLTYFPVSFLTLAAVLSAIDPNLEDAGLSLGASPGRVFRTVTLPLSTPGIANSVLLL
                     FASSLADFATPLVLAGHSFPVLPTQAYLQITGLYDLRGGAALSFLLLLPAMGVYALQR
                     FWVGRKNYVTISGKTGSRSGFKSKGKLAEGLVLGVCFAVTAFILYLYSLILWGALVKV
                     WGVDNSMTWENFRYVFTHGRKAIADTLSIAAVATPLGGLMAVVVGYIAQRKRFFGTRA
                     MEFFSMLNYALPGTVVGIAYVIAFNEKPILLTGTMTILVAAYMFRYHAAGIRAVIASL
                     HQIDPSIEEASASLGAGAARTFFRVTVPLVVPAVLMGMRYLFIHCMTAISATIFLVSV
                     KWSLLTTRILECMTELQFAQACAFSVVLILLVFGATAVLTGLTRFATRRISGEGGRG"
BASE COUNT          481 a          650 c          754 g          353 t
ORIGIN      
        1 ctggaagacg aagccgatat ccctcctgtt caccgggaca ttggtcacgt cctggccgtc
       61 gatggtgatg gttccaccgg agggggtttc gaaccccgcc accatcctga gggtcgtggt
      121 cttcccgcat ccggagggac cgagaaaagt gaccatctct cccggcgcca ccgtcatgga
      181 gacgttgtcc acagcccgga ccagttcccg gtccttccgg aatattttcg acacattcaa
      241 gagttcgagt gttacggcca tcagcctcgg cctccttcac cggaaatgcg cctcgttgcg
      301 aagcgggtca ggcccgtcag gacggcggtg gcgccgaaga cgagcagaat gagcacgacg
      361 gaaaaggcgc aggcctgggc gaactgcagc tccgtcatgc actcgaggat gcgggtcgtg
      421 agcaggctcc acttgacgga cacgaggaaa atggtggcgc tgatggcggt catgcagtgg
      481 atgaacaggt accgcatccc catgagcacc gcgggaacca ccagggggac ggtcaccctg
      541 aagaaggtcc tggccgcccc ggcgccgaga cttgccgacg cctcctcgat ggagggatcg
      601 atctggtgga gggacgcgat caccgcccgg atgcccgcgg cgtggtagcg gaacatgtag
      661 gccgccacga ggatggtcat ggtgcccgtg agcaggatgg gcttttcgtt gaaggcgatg
      721 acgtaggcga tgcccaccac cgttccggga agggcgtagt tcagcatgga gaaaaattcc
      781 atggccctgg tgccgaaaaa ccgcttccgc tgggcgatat agcctaccac cacggccatg
      841 agacctccga gaggcgtggc caccgcagcg atggacaggg tgtcggcaat ggccttgcgc
      901 ccgtgggtga acacgtaccg gaaattctcc caggtcatgg agttgtccac gccccagacc
      961 ttcaccaggg ctccccagag gatcagggaa tacaggtaca ggatgaaggc ggtgacggcg
     1021 aagcacaccc cgagcacgag cccctccgcg agcttgccct tgctcttgaa gcccgagcgg
     1081 cttcccgtct tcccgctgat ggtcacgtaa tttttccgtc caacccagaa gcgctgcagg
     1141 gcgtagaccc ccatggcggg aagaaggaga aggaaggaaa gggccgcccc gccccgcagg
     1201 tcatagagcc ccgtgatctg gaggtacgcc tgggtgggaa gcacggggaa gctgtggccc
     1261 gccagcacca ggggagtggc gaaatccgcc agggagctgg cgaagaggag cagcaccgag
     1321 ttggcgatgc ccggggtgga caggggcagg gtcactgtcc ggaaaacccg ccccggcgaa
     1381 gcccccaggg acagcccggc gtcctcgagg ttcgggtcga tggcggaaag gaccgccgcc
     1441 agggtgagga aggagacggg aaaataggtg agcgtctcgg acatccaggt accccagaat
     1501 ccgtagaaac tgaagttctc tatgccgaag aacttcagga ggatgccgtt gggccccagg
     1561 gaaagggtca gggcgatgct gctcgtgaag ggcggggaaa tgaggggcag cgtggtgacc
     1621 gctccgagaa accatttcag ccaggagggg aggggaatcc tggttaccgc gaaggcgtag
     1681 atgtacccca gcaccgtccc ggcaaacgaa acggcccccg cgagccagag gctgttcagg
     1741 aaggcctgcc ggtcgtacca gctgtcgaga accgccccga ggttcgcgaa ggaaaactgg
     1801 ccttccacca taaacgtcat aagaaaaagc cgaaggaccg ggtacaggac gaacaccgcc
     1861 agggaaatcc agatggcggc aagggcaggg agaagggccg ggtcccgttt gagcggcgac
     1921 accttctccg gagcggagag cgcggatggt cggttcatgg cgtcattctc cttgagtgag
     1981 ctgtaatgcc tgcagaacag ggaaaaagcc ccggcgggag acactcccgc cggggatggg
     2041 gtctgcgtcg gattccagaa aaagggccta cttgatgact tcgttgatcc agcggtcaac
     2101 gaaggccttg cggttctggc ccttccaggt cacgtccact tccaccaggc tgaccttgga
     2161 aaggtcaagg gcgggattgg tcacttccac gtcgccccgg gtggggatgt agttgatctt
     2221 cctgtccacc agcagctg
//
LOCUS       DAOTHD010000338         2235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_57664_length_2235_cov_2.802752, whole genome shotgun sequence.
ACCESSION   DAOTHD010000338 DAOTHD010000000
VERSION     DAOTHD010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57664_length_2235_cov_2.802752"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            500..1573
                     /locus_tag="P5219_06470"
     CDS             500..1573
                     /locus_tag="P5219_06470"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRX26435.1"
                     /translation="MKRCMLVLFVLTALIFSSAACAAPANPVLRVITWAGYAPQELLD
                     KFTAETGYKVEITLSNNEEMISKLRATRGGGFDLAQPSQDRISSVVEQYRIYQPIDYS
                     RLNEEQIDPSLLNAVKKNTLVDGKSYAVPHVYGTEGLVINRAKAPDVKDFKDLLDPKY
                     AGRVSYRLKRPTLIGMGFSLGHDPFALYGKPEEYQKFLDEMEKVLIAGKPSVKTYWDN
                     GDQLLQLLRSGEVWAASAWEQAGWKLHGENPDIDYLAPASGALAWVDTFAIPARSENL
                     EGAYAYIDFMLRPENAAFFTNRETYGTASKDAVNFLDPEIKANFTRGLPPEVLAKINW
                     YPTVPAGIEEMEGRTLDKVKAAR"
     gene            1653..>2235
                     /locus_tag="P5219_06475"
     CDS             1653..>2235
                     /locus_tag="P5219_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX26436.1"
                     /translation="MPFALSVHGLTKKFGNFTAVENISFDVEDGKFFSILGPSGCGKT
                     TLLRMIAGFYRQDGGTILFNGEDMSSVPPNRRPVNLVFQNLALFPMMNVFENVAFGLR
                     RRGEKGSGVARKVNDMLERVGLPDSGEKQISQLSGGQKQRIAIARCLVLDPAVLLLDE
                     PLGALDAKLREHMKVELKTLQANVGTTCVYITHD"
BASE COUNT          489 a          638 c          646 g          462 t
ORIGIN      
        1 tgcccttcat gggctttccg cgcgatttca atggctcgtt ccagtgttgg catgtttgaa
       61 acctcccttt cgttttgtgg ctggttgtgt cctgctccag aaaacggagc tgattacctt
      121 tcccggaaaa acgggaaagg aagactgtcc tgcttcggaa cactgggaaa aaggggatgg
      181 ttttcagcat gagggggaac tttttgcttt caatgagaga ataacatatc cgtcctgaaa
      241 tgggaatgtt ctgccccgga ccgatcgtga aacgccgccg gggccttccg caggcaagag
      301 aagtcataga gggatgtccc gagcgaatgc ttttttgttt cggctccgta acactttctg
      361 ccgttttcca ttgttttatt gacctcaaag tccattgata ctagaatcaa agccgccggg
      421 ttccatatac ggcattccgg ccatgggacg aaacaacggg aaagcggttc attatcggaa
      481 tcctcaggag ggatagtgga tgaaacgatg tatgcttgtg ctgtttgttc tgaccgcgct
      541 gattttcagc tccgcggcgt gtgccgctcc ggcgaacccg gtgctccgcg tcattacgtg
      601 ggcgggatat gccccccagg aactccttga caagttcacc gcagaaacag ggtacaaggt
      661 tgaaatcacc ctgagcaaca acgaggaaat gatcagcaag ctccgtgcca cccggggcgg
      721 cggctttgac ctcgcccagc cgtcccagga caggatctcg tccgtggttg agcagtacag
      781 gatttaccag cccatagatt attcccgcct aaacgaggag cagattgacc cctcccttct
      841 caacgccgtg aaaaagaaca cgctcgtgga cgggaagtcc tatgcggttc cccatgtcta
      901 cggcaccgag gggcttgtga tcaacagggc caaggcgccc gacgtaaagg atttcaagga
      961 cctgctcgat ccgaagtatg ccggcagggt gtcgtaccgg ctgaagaggc ccactctcat
     1021 cggcatgggg ttctccctgg gacacgatcc cttcgctctc tacgggaaac ccgaagagta
     1081 ccagaagttc ctcgacgaga tggagaaggt ccttatcgca ggaaaaccct cggtgaagac
     1141 ctattgggac aacggagacc agctgctcca gctcctccgt tccggagagg tctgggctgc
     1201 gtccgcgtgg gagcaggccg gatggaagct ccacggggag aatcccgaca tagactacct
     1261 cgctcccgca agcggcgccc ttgcatgggt ggacactttc gcgatccccg cccgttcgga
     1321 gaaccttgaa ggggcctacg cctacatcga tttcatgctc cgccccgaga acgctgcctt
     1381 cttcaccaac cgggagacct acggcaccgc ctcgaaggat gccgtgaact tcctcgatcc
     1441 ggagatcaag gccaatttca cccggggact tccgcctgag gtcctggcga agatcaactg
     1501 gtatccgacg gttcccgccg gaattgaaga gatggaaggc cggacgctgg acaaggtgaa
     1561 ggccgcacgg taaaaatcgt cagcaacgaa tcccgcgccg ccggcggcct ccagagggct
     1621 gccggcggca ctctgattga ggtgacctct ccatgccctt tgccctttct gttcacggtc
     1681 tcactaaaaa attcggaaat ttcaccgccg ttgaaaacat atccttcgac gtggaggacg
     1741 gaaagttttt ttccattctc gggccttcgg gatgcggcaa gacgacgctc cttcgaatga
     1801 tcgccgggtt ttaccggcag gacggaggga ccatcctgtt caacggcgag gacatgagca
     1861 gcgtccctcc gaacaggcgg ccggtgaacc tcgtcttcca gaaccttgcc cttttcccca
     1921 tgatgaacgt gtttgaaaac gtagccttcg ggctgaggcg gagaggagag aagggcagtg
     1981 gcgtcgcccg gaaggtgaat gacatgctcg agcgggtggg gcttcccgac tcgggagaga
     2041 agcagatcag ccagctttcg ggcggccaga agcagcgcat cgccattgcc cgttgcctgg
     2101 tgctcgaccc ggcggtactg ctgctcgatg agcccctcgg cgcccttgac gcgaaacttc
     2161 gggagcacat gaaagtcgag ctgaaaaccc tgcaggcgaa cgtggggacg acatgcgtct
     2221 acataaccca tgacc
//
LOCUS       DAOTHD010000339         2232 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_57768_length_2232_cov_2.903537, whole genome shotgun sequence.
ACCESSION   DAOTHD010000339 DAOTHD010000000
VERSION     DAOTHD010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57768_length_2232_cov_2.903537"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /gene="valS"
                     /locus_tag="P5219_06480"
     CDS             complement(<1..1341)
                     /gene="valS"
                     /locus_tag="P5219_06480"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRX26437.1"
                     /translation="MSDILEKMAKAYDPKPIEDKWYASWMEKGLFKARPGGDKSSAFS
                     IVIPPPNVTGSLHMGHAFNHTFQDILCRYKRMRGYNVLWLPGTDHAGIATQNVVERRI
                     AGEGKSRHDLGRDEFVRRVWEWKEEFGSRIINQMKKLGNSCDWDRERFTLDEGLSRAV
                     RTVFVRLYKKGLIYKGKYIINWCSRCHTALSDIEVEHEEHGGKFYHVRYPFVDGDGWL
                     IVATTRPETIWGDVAIAVHPRSEKAALAGRKVRVPLGGREIPVIEDIMVDPEFGTGCV
                     KITPAHDSNDFLVGQRHSLEQVQVIDEHGFMNDLAPGYEGLSIEEARKKAEEDLRNSG
                     FLEKTENMVHSVGHCYRCRTVVEPYLSEQWFVKAKPLAEAGVRAVEDGRIRWAPQQWE
                     KVYFQWMENIRDWCISRQLWWGHRIPAWTCEDCGHITVSENDPAACEKCGSTKIV"
     gene            complement(1368..1997)
                     /gene="yihA"
                     /locus_tag="P5219_06485"
     CDS             complement(1368..1997)
                     /gene="yihA"
                     /locus_tag="P5219_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRX26438.1"
                     /translation="MRVHWKVRLEATAFRPDQFPVQNVPEIAVAGRSNVGKSSLVNML
                     LGSRLAHVGATPGKTRSINFFAVESPAGPFRLVDLPGYGYASRSKGERNQWSALIDSY
                     VSNRESLALVLHLADFRHGLLANDTALQDWLSHLELPLLVVFTKGDKISKGARRGTLQ
                     KYIRNGLKSVDVPIITSAEKGDGIQDLRLFLEQYLTSGERPSPEGSPEE"
     gene            complement(1994..>2232)
                     /locus_tag="P5219_06490"
     CDS             complement(1994..>2232)
                     /locus_tag="P5219_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26439.1"
                     /translation="GIAMTGEISLQGKVLPVGGIRDKILAAKRQGIKNIILPAANRPD
                     VEEIPEWSRDGLSFRYVERVAEVFDFALERAEGK"
BASE COUNT          410 a          700 c          604 g          518 t
ORIGIN      
        1 gactatcttc gtggaaccgc acttttcaca ggcagccggg tcgttctcag acacagtgat
       61 atggccgcag tcttcgcagg tccacgcggg aattcggtgc ccccaccaga gctgccggga
      121 aatgcaccag tcccggatat tctccatcca ctggaaatac accttttccc actgctgcgg
      181 tgcccagcgt attctgccat cctccacggc cctgaccccg gcctcggcaa ggggctttgc
      241 cttcacgaac cactgctcgg agagataggg ctccaccacg gtgcggcacc tgtagcagtg
      301 ccccacggaa tggaccatgt tctccgtctt ttccagaaat ccggagtttc ggaggtcttc
      361 ttcggccttc tttctcgcct cttctatgga aagcccttcg taccccgggg ccaggtcatt
      421 catgaagcca tgctcatcaa tcacctgcac ctgctcgagg ctgtggcgct gaccgacgag
      481 aaaatcgttg gagtcatggg ccggcgtaat cttgacgcag ccggtgccga actccggatc
      541 gaccatgatg tcctcgatca cggggatttc ccgcccgccg aggggcaccc ggactttcct
      601 tccggcaagg gccgcctttt ccgacctcgg gtgaacggct atggccacgt ccccccagat
      661 ggtttccggg cgtgtggtgg cgacgatcag ccagccgtcg ccgtcgacga agggataccg
      721 gacatggtaa aacttccctc cgtgctcttc gtgctccacc tcgatgtcgg agagggctgt
      781 gtgacaccgg gagcaccagt tgatgatata tttcccctta taaatgagcc cttttttgta
      841 caggcggaca aagaccgtcc tgacagcccg ggagagcccc tcgtcgaggg taaaacgctc
      901 ccggtcccag tcgcaggaat tgccgagctt cttcatctgg ttgatgatac ggctgccgaa
      961 ctcttccttc cactcccaga ccctccggac gaattcgtcc cgtccgaggt cgtgccgtga
     1021 ttttccctcc cccgctatcc tccgctccac cacgttctgg gttgcgattc ccgcgtggtc
     1081 cgttccggga agccagagga cgttgtatcc tctcattctc ttgtacctgc agagaatatc
     1141 ctgaaaagtg tggttgaagg cgtgccccat gtgaagggag ccggtcacgt tgggaggagg
     1201 aatgacgatg gaaaaggcac ttgacttgtc gccgccgggc cgtgccttga aaagtccttt
     1261 ttccatccac gaggcatacc atttgtcctc tataggtttg ggatcgtacg ctttggccat
     1321 tttttccagg atatcagaca tgacgaaact ccctccgttg cgtagattta ctcttccggt
     1381 gatccctctg gcgacggccg ttctcctgag gtgaggtact gctcgagaaa aagcctcagg
     1441 tcctgaatgc cgtctccctt ttccgccgac gtgatgatcg gtacgtccac cgatttcagg
     1501 ccgttccgga tatatttctg gagcgtacct cttctggcgc cctttgaaat tttatcccct
     1561 ttggtgaaaa ctacaagcag ggggagttcc aggtgggaaa gccagtcctg gagagccgtg
     1621 tcgttggcga gcagcccgtg cctgaaatcg gcgagatgaa gcacgagcgc cagcgattcc
     1681 ctgttcgaaa catagctgtc gatgagggcc gaccactggt tccgctctcc cttgcttctg
     1741 gacgcatacc cgtagccggg aaggtccacc agcctgaagg gacctgccgg actttccacg
     1801 gcgaaaaagt tgatgctcct cgtttttccc ggagttgcgc ccacgtgggc caggcggctt
     1861 cccagaagca tgttcacgag acttgacttg ccaacgttcg acctgccggc cacggctatt
     1921 tcgggaacgt tctgaacagg gaactggtcc ggccgaaatg ccgtcgcttc gaggcggacc
     1981 ttccagtgta ccctcatttt ccctccgctc tctcgagggc gaagtcgaac acttccgcca
     2041 cccgttcgac gtacctgaag gaaaggccgt ccctggacca ttccgggatt tcctccacat
     2101 cgggcctgtt ggccgccgga aggatgatgt tctttatccc ctggcgcttc gccgccagga
     2161 tcttgtcccg tatccccccc accggaagga cttttccctg aagggagatt tcccccgtca
     2221 tggcgattcc cg
//
LOCUS       DAOTHD010000340         2216 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_58348_length_2216_cov_2.590005, whole genome shotgun sequence.
ACCESSION   DAOTHD010000340 DAOTHD010000000
VERSION     DAOTHD010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58348_length_2216_cov_2.590005"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="P5219_06495"
     CDS             complement(<1..1061)
                     /locus_tag="P5219_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRX26440.1"
                     /translation="MKKKIIIVGSGGAGLTAAVAAAKQGADVLLLSKTACGDANCTAY
                     SGGMFSLSSGTVTPDDHFRRILETGRGVNDEKLVRILAEESEKTLRTLAEWGITLKIN
                     SSGQASVRKSAPSEIMGGAGLTRELAALAKAEGVSILENCAATKILAGERGVEGVEWV
                     NWKTGKQYQAPASAVILATGGGGGIYSRTDNPARMTGDGYALALEAGLDLADMEYVQF
                     YPLGWKDPAFPLWMVGLTILDYIPVTDDSGREFLREAILSWGLSSGKEANYFARDKCA
                     IFLSRHELQGGKTLLHLENLGEEMLTIPDVRVSLMVDLPPERRTGPVELSPIQHYFTG
                     GIPIDTGGRLEIRGNGSEGG"
     gene            complement(1139..>2216)
                     /gene="fusA"
                     /locus_tag="P5219_06500"
     CDS             complement(1139..>2216)
                     /gene="fusA"
                     /locus_tag="P5219_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048751.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRX26441.1"
                     /translation="TIFNVNKGEEERISAFKLMTGKEGRDVKEITVGDILAIPKLHST
                     QVGDSLSVKGSTVTFPPIDFPKPVYSVAIIAKSRADEDKLGNAIHKTLEEDPSLTFDK
                     NAETNDSVLSGMGDMHIDIVLSRIKERYGVDLETKTPQVPYRETIRKTAEAQGKHKKQ
                     TGGHGQYGDVHVRYTPLERGEGFVFEDKIVGGAIPKGFIPAVEKGLREALVKGPLASF
                     PVVDFKATLFFGSYHDVDSSEMAFKLAARLSFKKGILEANPVLLEPIMNVEVTVPEEY
                     LGDVMGDFNGRRGRILGIDSKGHQQVVKAQCPLAEMFRYAIILRSMTSGRGTFTMEYD
                     HYEEVPAEIAKKVIAAHKQEDEEE"
BASE COUNT          377 a          674 c          672 g          493 t
ORIGIN      
        1 cccccttcag aaccattgcc gcggatttcc agccgtcccc cggtatcgat gggaatcccc
       61 cccgtgaaat agtgctggat gggcgaaagc tccaccggcc cggtccgccg ctccggcgga
      121 aggtccacca tgagggacac ccgtacatca ggaatagtca gcatctcttc ccccaggttc
      181 tccaggtgaa gcagggtctt tcctccctgc agctcgtgac gggagaggaa gatggcgcat
      241 ttatcccgtg cgaagtagtt cgcctctttc cccgaggaga gcccccagga gagaatggcc
      301 tcccggagga attcccggcc gctgtcgtcg gtaacgggaa tgtagtccag gatggtgagc
      361 cccaccatcc acagggggaa tgcgggatcc ttccagccca gagggtagaa ctggacatac
      421 tccatgtctg cgaggtccag tcccgcctcg agagcgagag cgtatccgtc tcccgtcatc
      481 cgcgcgggat tgtcagtccg tgagtagatt ccgccgccac cgccggtggc aagtatgacc
      541 gcggaagcag gtgcctggta ctgtttcccg gtcttccagt tcacccactc caccccctcc
      601 acacccctct cgccggcaag aattttcgtt gcggcacagt tttcgaggat ggagactcct
      661 tccgcctttg ccagggctgc aagttccctg gtcaggcctg ctcctcccat gatctcggaa
      721 ggggcagatt tccggaccga ggcctgcccc gaggagttga tcttcagagt gattccccat
      781 tccgcaaggg ttctcagggt tttctccgat tcttccgcga ggatcctgac gagtttttcg
      841 tcattcacgc cccgcccggt ttcaagaatc cgccggaagt ggtcgtcagg ggtcacagtt
      901 ccggaggaga gggaaaacat gcccccggag taagcggtgc agtttgcgtc cccgcaggca
      961 gtcttcgaga gcagcaggac atccgccccc tgttttgccg ccgcgacggc ggcggtgagg
     1021 cccgctccgc cggacccgac aatgatgatt tttttcttca tggttctcct cctccctatg
     1081 aagggagccc cgcggcatgc gcggggctcc gaaaacggtt cttttcgagt gcgtcgagct
     1141 attcctcttc gtcttcctgc ttgtgggccg cgatgacctt cttcgcgatc tcggcgggaa
     1201 cttcctcgta gtggtcgtat tccatggtga aggttcccct gccggaggtc atggaacgga
     1261 ggattatggc gtaccggaac atctccgcga gggggcactg tgccttgacc acctgctggt
     1321 gccccttgct gtcgatgccg aggatccgtc ccctccgtcc gttgaagtcg cccatgacgt
     1381 cgccgaggta ctcctcggga acggtgactt ccacgttcat gatgggctca agaagaacgg
     1441 ggttcgcctc gagaataccc ttcttgaagg acagccgcgc ggcaagcttg aaggccattt
     1501 cggagctgtc cacgtcgtgg taggagccga agaagagggt ggccttgaaa tccaccacgg
     1561 ggaagctggc aaggggtccc ttgacgaggg cttcccggag gcccttctcg acggcgggaa
     1621 tgaatccctt gggaatggcg ccgccgacaa ttttatcctc aaagacgaag ccctctcccc
     1681 gctcaagggg agtgtagcgg acatggacgt cgccgtactg gccatggccg cccgtctgct
     1741 tcttgtgctt gccctgggct tcggcggtct tccggatggt ctcccggtag ggaacctggg
     1801 gggtctttgt ttcaaggtcc acaccgtacc gctccttgat gcgggagagg acgatgtcga
     1861 tgtgcatgtc gcccattccg gagagcacgc tgtcattggt ctcggcgttc ttatcaaagg
     1921 tgaggctggg gtcttcttcg agggtcttgt ggatggcgtt gcccagcttg tcttcgtcgg
     1981 cccggctctt ggcgatgatg gccacgctgt agacgggctt cgggaagtcg atgggcggaa
     2041 aggtgaccgt ggaccccttc acggaaagac tgtcccccac ctgggtgctg tggagcttgg
     2101 gaatggcgag gatgtcgccc acggttatct ccttgacgtc cctgccttct ttgccggtca
     2161 tcaacttgaa ggcgctgatc cgttcctctt cgcccttgtt cacgttgaaa atggtg
//
LOCUS       DAOTHD010000341         2207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_58650_length_2207_cov_3.396375, whole genome shotgun sequence.
ACCESSION   DAOTHD010000341 DAOTHD010000000
VERSION     DAOTHD010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58650_length_2207_cov_3.396375"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="P5219_06505"
     CDS             complement(<1..190)
                     /locus_tag="P5219_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HRX26442.1"
                     /translation="MDIRFVTRGVELKDDLKDYMENKMSKLERFFSRIIDGQVVMSFG
                     RGLFTVEITSNANGVIMRG"
     gene            336..1526
                     /locus_tag="P5219_06510"
     CDS             336..1526
                     /locus_tag="P5219_06510"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRX26443.1"
                     /translation="MYISERARKMEPSATLAVTAKAKSLKREGKPVISFGAGEPDFDS
                     PPSAIRYAEEAMKKGQTHYTPGTGIPELREAVCAYYKDHFGLEYSPANVVVGAGAKPL
                     LYEALGCVIDPGDEVLVFTPAWVSYVEQIRFFDGKAVLVDTSKTEFIPRYDDVKKAIT
                     PRTRAMIVNTPNNPTGAIYDEQCLRDLGKLAVEHNIVIIFDEIYERLVYCGEKHHQIL
                     NLVPEAKDLTIIINGVSKAFAMTGWRIGYALGPSSIMGYLGDLQGHITSNACSVAQWA
                     SVGALKEADQDVRTMHKAFSKRRDLIVDLMKDMPYISFTEPKGAFYVWFNVDRILGKS
                     WNGKVLSDDAELCKVFLESKYVALVPGSAFMAPGNVRISYSNSEEEIREGMRRFKEFL
                     EELK"
     gene            complement(1585..>2207)
                     /locus_tag="P5219_06515"
     CDS             complement(1585..>2207)
                     /locus_tag="P5219_06515"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HRX26444.1"
                     /translation="VEANEFLRSGEWVTWHPDLLLGQDVSGSVLGIVGMGKIGQAVAR
                     RAAGFSMKILFFNRSPKPDLEKELGARQVSMDELLRESDFVSLHCPLNAETKGLIGER
                     ELRMMKKSSILINTSRGPVVDQNALYRACSERWIWGAGLDVFEKEPVPLDEPLLKLKN
                     VTTIPHLGSATIKAREGMARKAAENLLAALEGRKPADLVNPSAWKE"
BASE COUNT          511 a          625 c          572 g          499 t
ORIGIN      
        1 gccctctcat gatgaccccg ttggcgttgg aggtgatctc cacggtaaac agtccccgcc
       61 cgaaactcat cacaacctga ccatcgatga tgcggctgaa aaatctctca agcttggaca
      121 tcttgttttc catgtagtcc ttcaggtcgt ccttcagttc gactccacgg gtgacaaatc
      181 gtatgtccat gcaaatccct ccattcaggc tcagtaatta tgctatcatt ctaacaccgg
      241 gggagattct gggcaacaaa aggaataagc ccctcatcag cccccggatg gggtataatg
      301 atgctaccac catattgtgc aggaggtatt atgtcatgta catttccgag agagcccgaa
      361 agatggaacc atcagcaacc ctcgccgtta ctgcaaaagc caagagtctc aaacgggaag
      421 gaaaaccggt tatttccttc ggcgccggcg aaccagattt cgactctccc ccttcggcaa
      481 tccgctatgc tgaagaagca atgaagaagg gacagaccca ctataccccg ggaacgggca
      541 ttccggagct tcgggaggcg gtctgcgcct attacaaaga ccatttcggc cttgaatatt
      601 cccccgcaaa cgttgttgtc ggtgcggggg ccaagccgtt gctttacgag gctctgggat
      661 gtgtcatcga tcccggcgac gaggttttgg tgttcacccc cgcatgggtg agttacgtgg
      721 agcagatacg ctttttcgac gggaaagcgg ttctcgtgga cacgagcaaa acagaattca
      781 tcccccggta cgatgacgta aaaaaagcca ttactccgag aacccgtgcc atgatagtca
      841 acacgccaaa caacccgacg ggcgccattt atgacgagca gtgcctcagg gatctcggaa
      901 aactcgctgt ggaacacaat atcgtcatca tcttcgacga gatttatgaa cgccttgtct
      961 actgcggaga aaagcaccac cagatcctga acctcgtacc ggaggcaaag gacctcacca
     1021 ttatcatcaa cggagtcagc aaggccttcg ctatgaccgg ctggcggatc ggatacgctc
     1081 tcggtccctc gtccatcatg ggatatctcg gcgacctcca gggtcatatt acctctaacg
     1141 cgtgctccgt ggcccagtgg gcctccgtcg gtgctctgaa agaagccgac caggatgtcc
     1201 ggaccatgca caaggccttc agcaaaagac gggacctcat cgttgacctc atgaaggata
     1261 tgccctacat ctccttcact gaacccaagg gtgcgttcta tgtgtggttc aacgtggaca
     1321 ggatccttgg aaaaagctgg aacgggaagg ttctttccga cgatgccgaa ctgtgcaagg
     1381 tgtttcttga atccaagtac gtcgctcttg ttcccggcag cgctttcatg gcaccgggca
     1441 acgtacggat ttcctactcg aactccgaag aggaaatcag agagggtatg agacgcttta
     1501 aagagttcct cgaagagctg aagtagacag gaacggccga tgaagaaacc gtcccctccg
     1561 gagtttcgcc ggaggggacg gtttttattc cttccaggct gacgggttta ccaggtcggc
     1621 cggttttctt ccttccaggg cggccaggag gttttcggcg gcttttcgtg ccatcccttc
     1681 cctcgctttt attgtagcgc tcccgaggtg aggtatggtg gtgacgtttt tcagcttcag
     1741 caggggctcg tccaggggga caggctcttt ttcgaagaca tcgagcccgg cgccccagat
     1801 ccatcgttcc gaacaggccc tgtagagagc gttctggtcc accacaggtc cccttgacgt
     1861 gttgatgagt atggaagatt tcttcatcat tcggagctcc cgctccccga taaggccttt
     1921 tgtttccgca ttcaggggac aatgaaggga aacgaagtca ctctctctca gcagttcatc
     1981 catggacacc tgccgtgcgc caagctcctt ttccaggtcc ggtttcggag atcggttgaa
     2041 aaacaggatc ttcatggaaa acccagccgc tcgtctcgcc acggcctgac cgatttttcc
     2101 cattcccacg atgccgagaa cagatccgga tacatcctgt ccgagaagca ggtcaggatg
     2161 ccaggtgacc cattcccccg aacggaggaa ctcgttcgcc tccacca
//
LOCUS       DAOTHD010000342         2205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_58724_length_2205_cov_2.529302, whole genome shotgun sequence.
ACCESSION   DAOTHD010000342 DAOTHD010000000
VERSION     DAOTHD010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58724_length_2205_cov_2.529302"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="P5219_06520"
     CDS             complement(<1..820)
                     /locus_tag="P5219_06520"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRX26445.1"
                     /translation="MKKPVVLLLAAVLALVFVLPVQAAPLKLVAAHNQTSQENPYQYG
                     MLKFKEVVEKLSNGEISVDVHAGTIGTNEDELVEKLKLGAADVVVASPGFMTKIGIPE
                     VDLFSLLYLFNSFDHWEKAVDGEAGQTLAKIINEKSKNSFRIAAYWSAGVRNYYGKKP
                     INTMDDLKGMKIRTQMSGVVADFWKQTGAIPTQVAWGELYQALQQGIVDCAENDYTNF
                     SLLDHHKTVNGKFITETEHDFTTRFVLMNGRKFDALTDQQKEWITEALKQATAEE"
     gene            complement(879..1883)
                     /gene="yiaK"
                     /locus_tag="P5219_06525"
     CDS             complement(879..1883)
                     /gene="yiaK"
                     /locus_tag="P5219_06525"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF009750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HRX26446.1"
                     /translation="MRVPYDEMKGTFEKILLKYGFPEKTAARAAVMFTDNSCDGVASH
                     GLNRFPRVLSYISKGHIDPHAEPAPEASFGALERWNGNRGMGCTNAAAAMDRAMELAR
                     RSGLGCVALGNTNHWMRGGSYGYQAAKAGFMAFCWTNTMPNMPPWGAKECRVGNNPLV
                     MAFPWKDAPVVIDGALAQYSYGALDGYRMAGKQLPFPGGYDREGKLTTDPAAVLETWR
                     VLPIGFWKGSGYSLVLDLIGSVLSKGNSVHQVGKLGDEIAVTQVFLAFDVENTSGKEY
                     AETAAASILEDFRSSEPAEPGGSYFYPGEKAALTRRENLEKGIPVVEDIWYDLLKELN
                     "
BASE COUNT          409 a          635 c          669 g          492 t
ORIGIN      
        1 gctcttcggc cgtggcctgc ttcagggcct cggtgatcca ttccttctgc tggtcggtga
       61 gggcgtcgaa cttcctgccg ttcatgagaa cgaagcgggt ggtgaagtcg tgctccgtct
      121 cggtaatgaa cttgccgttg accgtcttgt ggtggtcaag aaggctgaag ttggtgtagt
      181 cgttctcggc gcagtccaca atgccctgct gcagggcctg gtagagctcg ccccaggcaa
      241 cctgggtggg gatggcgccg gtctgcttcc agaaatcggc cacgacgccg gacatctggg
      301 tgcggatctt catgcccttc aggtcgtcca tggtgttgat gggcttcttg ccgtagtagt
      361 tacggacgcc cgcggaccag taggcggcga tgcggaaaga attcttggat ttttcgttga
      421 tgatcttggc gagggtctgt ccggcttcgc cgtccacggc cttctcccag tggtcgaaac
      481 tgttgaagag gtacagcagg gagaacaggt ccacctcggg gatgccgatc ttggtcatga
      541 aaccgggaga ggcgacgact acgtcggcgg cgccgagctt cagcttctcc acgagctcgt
      601 cctcgttggt gcctatggtg ccggcgtgga cgtccacgga gatctcgcca ttggagagct
      661 tctcgaccac ttccttgaac ttcagcattc cgtactggta ggggttctcc tgggatgtct
      721 ggttatgggc cgcaaccagc ttcaggggag cggcctgcac gggcaggacg aaaacgagcg
      781 caagaacggc tgcaaggagc agtacaacag gttttttcat atttggtacc cctttcgtat
      841 gtgaattttt gcgggtttgc cgcaaagtgt ttccggtgtc agttcagttc cttcagcagg
      901 tcgtaccaga tgtcctccac cacggggatg cccttttcca ggttttccct ccgggtgagg
      961 gccgcctttt cgcccggata aaaatagctt ccacccggct ccgccggctc ggaactcctg
     1021 aaatcctcca ggatggacgc cgccgctgtt tcggcatatt cctttccgga ggtattttcc
     1081 acgtcaaagg cgagaaaaac ctgggtcacg gcgatttcat caccgagctt ccctacctgg
     1141 tggaccgagt ttcccttcga gagcacggac ccgatgagat ccagcaccag ggaatagccc
     1201 gagcccttcc agaacccgat gggcaggaca cgccaggtct ccaggacggc cgccggatcg
     1261 gtggtgagct ttccctcccg gtcatagccg ccggggaagg ggagctgctt tcccgccatc
     1321 cggtatccgt cgagggcgcc gtaggagtac tgggccaggg cgccgtcgat gaccacggga
     1381 gcgtccttcc agggaaaggc catgaccagg ggattgtttc ccacccggca ttcctttgcc
     1441 ccccaggggg gcatgttcgg catggtgttg gtccagcaga aggccatgaa gcccgccttc
     1501 gccgcctggt acccgtagga gccgcccctc atccagtggt tcgtgtttcc gagagccacg
     1561 catccgaggc cggaccgccg tgcgagttcc atggccctgt ccatggccgc cgccgcgttg
     1621 gtgcacccca ttccccggtt gccgttccag cgctccaggg cgccgaagga tgcctcgggg
     1681 gcaggctcgg catggggatc gatgtgtccc ttcgaaatgt aggacagcac ccggggaaaa
     1741 cggttcaggc cgtgggaagc gacaccgtcg cagctgttgt cggtgaacat gacggccgcg
     1801 cgggccgccg ttttctcagg gaagccgtac ttgaggagga ttttctcgaa agtgcccttc
     1861 atttcgtcgt agggtacgcg catggacgtc acacctttct cctatactga aaaaaagaga
     1921 gcgatttcgt gttgtcgcac gaattccata ttaccgacca agaggaaatc cgtcaatgtt
     1981 cgacagagca gaattacgtt ctgtcagata aaacaccctg gagcccatcc tctatgagaa
     2041 tgagcgttct tcattcctga ctgtcagggc atttctccgt gagagctcgt gagagattcg
     2101 aaggtatgct tcataaattc tctggctgcc cagttcagaa aaaaaccttt tttccatgcc
     2161 agggcatatc ccgacgaaag gggaggggtt cccaccgcga gggcc
//
LOCUS       DAOTHD010000343         2197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_59038_length_2197_cov_3.037815, whole genome shotgun sequence.
ACCESSION   DAOTHD010000343 DAOTHD010000000
VERSION     DAOTHD010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59038_length_2197_cov_3.037815"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="P5219_06530"
     CDS             complement(<1..457)
                     /locus_tag="P5219_06530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRX26447.1"
                     /translation="MEWKNTADVVIIGGGIIGVSTAYYLAQNGMKNIVLIEKGTICGG
                     STGRCGAGIRAQWGTEMNCRLGIAALDIFEQLDQDLGMSVGLNQGGYLLVAYSEKELD
                     TLRKGMELQNSLGIKSRQITLREAREICPGLAADDAVGFFFHGRDGHADP"
     gene            complement(457..747)
                     /locus_tag="P5219_06535"
     CDS             complement(457..747)
                     /locus_tag="P5219_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRX26448.1"
                     /translation="MEKEHPGSEEIYVCRCEEVTLEEIRTWIDRGYDTVEELKRVLRV
                     GMGPCQGRGCQDILMRELAKKTGKPITEIEPATVRPPAKPIKIGLLAEEVEE"
     gene            complement(750..1256)
                     /locus_tag="P5219_06540"
     CDS             complement(750..1256)
                     /locus_tag="P5219_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HRX26449.1"
                     /translation="MTDALDLFNSGVLTGEREGAVLPDRKLWESKKGGVVIIECPQRI
                     PCNPCHTSCPTGAIRPFADINDTPKVDHSLCTGCTLCVASCPGLACFVIDLTFSEDKA
                     LFKLPYEMLPLPEKGQHVQCLNRTGECIAEGTVENVTQPKKDRTYVIHISAPKGIVSE
                     FRTIRVVK"
     gene            complement(1249..>2197)
                     /locus_tag="P5219_06545"
     CDS             complement(1249..>2197)
                     /locus_tag="P5219_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRX26450.1"
                     /translation="KDEYAGTRGFKIADILLSEIDSCKENVEIHTNTTITGFYSEDGT
                     FTAMRGEEKYFHIKAKKTIVATGAQERLIPFPNNDLPGVYGAGAVQTLMNVYGVVPGK
                     RVLMVGAGNIGLIVSYQLMQAGVEVAAVVEAAPRVGGYWVHAAKIRRMGVPILLRHSI
                     RKAVGDQVITGAYITALNDRFEPEGEETYVDCDVICIAVGLSPTTEILSQAGCKMMYV
                     PQLCGLVARRDRTMKTSNPDFFTAGDASGIEEASAAMVEGRIAGLSAAADLGYPTEHG
                     KLDEYWGRLQALRSGEAGARICQGLECVLVNRWEDVSDD"
BASE COUNT          427 a          623 c          582 g          565 t
ORIGIN      
        1 agggatcggc atgaccgtct cttccgtgga aaaagaaccc cacggcgtcg tcggcggcaa
       61 gccccgggca gatttccctg gcttcccgca gggttatctg cctcgacttg attccgaggg
      121 aattctggag ttccattccc ttcctgagag tatccagctc tttttcgctg taggcgacga
      181 gcaaatagcc gccctggttg agtcctacgg acattcccag atcctggtca agctgttcaa
      241 aaatgtcaag agccgcaatt ccgagcctgc agttcatttc cgtgccccac tgggcccgta
      301 tgcctgctcc gcatcgaccg gtggagccgc cgcagatggt tcccttttcg atgaggacga
      361 tgttcttcat gccgttctgg gcaagataat aggcagttga tacaccgatg atcccgcctc
      421 ctatgataac gacatcggcg gtgttcttcc attccatcat tcttcaacct cctctgcgag
      481 aagaccaatt ttgattggtt ttgcaggagg tcgaacggtt gccggctcta tttcggtgat
      541 tggctttcca gttttttttg caagctctct cataagtata tcctggcagc cccttccctg
      601 gcagggaccc atgccgaccc ggagaactct cttaagctct tccacggtat catatcccct
      661 gtcgatccac gtgcggattt cctcgagagt tacctcctcg cagcggcaga cgtagatttc
      721 ttcagatccc ggatgttcct tctccatggt catttcacca ccctgattgt ccggaattcg
      781 cttactatcc cttttggagc cgaaatatga atgacgtagg tcctgtcttt ttttggctgg
      841 gttacgtttt ccacagttcc ttccgcaatg cattcccctg ttctgttaag gcactggacg
      901 tgctgtccct tttcagggag agggagcatc tcatagggaa gcttgaaaag ggctttgtct
      961 tccgaaaaag tgaggtcgat tacgaagcac gcaagtcccg gacaggaagc gacgcagagt
     1021 gtgcatcccg tgcacagcga atgatcaacc ttcggagtgt cgttgatgtc ggcaaaggga
     1081 cggatcgctc cggtagggca gctggtatgg caggggttgc agggaatgcg ctgggggcat
     1141 tcgatgatga cgactcctcc ctttttcgat tcccagagtt tgcggtccgg cagaacggct
     1201 ccttcccgct ctcctgtgag cacaccgctg ttgaagaggt cgagagcatc agtcatcgga
     1261 tacatcctcc catctgttga caagcacgca ttcgagcccc tggcagatac gcgctccggc
     1321 ctctcctgac ctgagagcct ggagacgtcc ccagtactcg tcaagcttac cgtgttccgt
     1381 ggggtaccca aggtccgcag cagccgaaag accggctatt cttccctcaa ccatggcggc
     1441 gctggcctct tcgataccgc ttgcgtctcc ggcggtgaaa aaatcggggt ttgaggtttt
     1501 cattgtcctg tcacgtctcg ccacaagacc gcacaactgg ggaacataca tcattttgca
     1561 tccggcctgg gaaagaatct cagtggtcgg tgaaaggccg acggcaatgc agatgacatc
     1621 acagtcaacg tacgtttctt ctccctccgg ctcgaaacgg tcgttgagcg cggttatgta
     1681 tgctcccgtg atgacctggt ctcccacggc cttccttatg gagtggcgaa gaaggatggg
     1741 cacgcccatc cggcggattt ttgcggcatg gacccagtat ccgccgaccc tgggcgcagc
     1801 ttcgacaacg gcggcgactt ccacgcccgc ctgcatgagc tggtaactta cgatcaggcc
     1861 gatattgccc gctccgacca tgaggacccg tttcccgggg actaccccgt agacgttcat
     1921 cagggtctga acggctcctg cgccgtaaac gccggggaga tcattgttgg ggaacggaat
     1981 gagccgttcc tgggcgcctg tagccactat tgttttttta gctttgatat ggaagtattt
     2041 ttcttctccc cgcatggcgg tgaatgttcc gtcctctgaa tagaagccag tgatggttgt
     2101 attggtgtgg atttcaacgt tttccttgca cgagtctatt tcgctcaaga gaatgtcggc
     2161 gattttaaat cccctggttc ctgcatattc gtccttg
//
LOCUS       DAOTHD010000344         2196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_59074_length_2196_cov_3.485754, whole genome shotgun sequence.
ACCESSION   DAOTHD010000344 DAOTHD010000000
VERSION     DAOTHD010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59074_length_2196_cov_3.485754"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="P5219_06550"
     CDS             <1..293
                     /locus_tag="P5219_06550"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HRX26451.1"
                     /translation="PIVVGVGVGSDFEGVAELAKRALLRPAESSSSHPRYAALEAELL
                     RKINDLGIGAAGYGGTVTALGVNIEWAPTHIACMPVAVNLCCHANRHCSAEI"
     gene            305..868
                     /locus_tag="P5219_06555"
     CDS             305..868
                     /locus_tag="P5219_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HRX26452.1"
                     /translation="MEKCRRLNAPFLEKEASNLRAGDEILLSGVIYTARDAAHKRMSE
                     ALARNEKLKVDLSGAVIFYAGPAPARPGEPMGSIGPTTSYRMDPYTPLLLELGVKGMI
                     GKGERSEDVVSSMKKNGAVYLGATGGIAALLARTIVASRVLDYEDLGPEALRELEVRD
                     MPLVVLIDSLGNSMYREGPAKYGKNGK"
     gene            complement(875..1558)
                     /locus_tag="P5219_06560"
     CDS             complement(875..1558)
                     /locus_tag="P5219_06560"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRX26453.1"
                     /translation="MASRIFHSLGDQVFETIKELIINNTYHPGVVLQIDKLAGEFGVS
                     TTPVREALLRLEGIGLVDIERNKGAVVTQVSEKRSRYVWEFRRILEAKVARDAAIGCT
                     DEEISAVETKLKKVLEHPDDFSVYQDSDIALHGLLSSHTENPLILESLANLSVHARRI
                     RYFAENGPFREEVIEQVTREHNEIIAALREHNPDKVEAIVTRHLINAEERTKKALSEI
                     SGGADNDGK"
     gene            1836..>2196
                     /locus_tag="P5219_06565"
     CDS             1836..>2196
                     /locus_tag="P5219_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRX26454.1"
                     /translation="MFAQSKKMEAVPFSPIRKIFAEVDKLKAEGVDIITLGIGEPDFD
                     TPAHITEAMAAATRNGATHYTANKGIVQLREAVCRKLKVEDGLEYDPEEIICTVGVSE
                     GVYVSLSSFLDPGDEVLV"
BASE COUNT          497 a          588 c          619 g          492 t
ORIGIN      
        1 cgcccatagt ggtcggcgtg ggggtgggaa gcgatttcga gggagtagcg gaactcgcga
       61 agcgggctct tctcaggcct gcggaaagct ccagcagtca ccccaggtat gcggctctcg
      121 aggctgaact gcttcgaaaa atcaatgatc tcggcatcgg agcggcagga tacggcggta
      181 ccgtcaccgc cctcggagtc aatattgagt gggccccgac ccacatagcc tgcatgcccg
      241 tggcggttaa cctctgctgt cacgccaacc ggcactgcag cgcggaaatt taggagggga
      301 cagggtggaa aaatgcagaa ggctcaatgc gccttttctg gaaaaggaag cctcgaacct
      361 cagggcgggg gacgagattc ttctttcagg ggtgatttat accgcacggg atgctgctca
      421 caagcggatg tcagaagcac ttgcccggaa tgaaaagctg aaagtggacc tgtccggagc
      481 ggtcatattc tatgcgggac cggcccctgc acggcccggc gagcccatgg gctccatcgg
      541 gccgacaacg agctaccgca tggatcccta cacccctctg cttcttgagc ttggagtcaa
      601 ggggatgatc gggaagggcg aacggtcgga ggacgtcgtg tcgagcatga aaaaaaacgg
      661 ggccgtttac ctcggggcaa ccggaggaat cgcggccctg ctggcacgga caatagtcgc
      721 ttccagggtt ctcgattatg aagatcttgg gcctgaagcc ctgagggaac ttgaggtccg
      781 ggacatgccg ctggtcgtcc tcattgacag tctgggcaac agcatgtacc gggaaggtcc
      841 ggcgaaatac ggaaaaaacg ggaaatagga aagcctattt cccgtcgttg tccgcccctc
      901 cggaaatttc ggagagggct ttttttgtgc gttcctcagc gttgatgagg tgccgtgtca
      961 cgatggcctc aactttgtcg gggttgtgtt ccctgagggc ggcgatgatt tcgttatgtt
     1021 ccctggtcac ctgttctatg acttcttccc ggaaagggcc gttttcagcg aagtaccgga
     1081 tccttcgcgc atggacgctg agattggcca gcgattccag gatcagggga ttctccgtat
     1141 ggctcgaaag aagaccgtga agggcaatgt cggaatcctg gtatacggag aaatcatcgg
     1201 gatgttcgag gacctttttc agcttggtct ccacagcgct gatttcttcg tccgtacatc
     1261 cgatggccgc gtcacgggct acctttgcct cgagaatgcg ccggaattcc cacacgtagc
     1321 gtgagcgctt ttcacttacc tgggtgacca cggctccctt gtttctttca atgtccacga
     1381 ggccgattcc ctcaagcctg agaagcgctt cccgaaccgg agtggtgctg acgccgaact
     1441 cccccgcaag cttgtcaatc tggagaacga cgcccggatg atacgtgttg ttgatgataa
     1501 gctccttgat tgtttcaaaa acctggtcgc ctaaagaatg aaaaattcga cttgccaaag
     1561 aacccaactc ctctcccgga atgccacaac ttcgtatttt accatttcag gtggaatttt
     1621 ccataacctc gtccatttcc tttccttcca aatgaaacaa agcaactcat ttcaaataat
     1681 ataattgaca taaaacagac tgcatgatag attttttaca aggtttgccc tgccgcgcgc
     1741 tcttgacatc gattattctc cgcggctctc ggggagcggg tttatcgacc tcgacaggga
     1801 cctcaatttc acccccggtt aaggaggatt tttggatgtt cgcccaatca aaaaaaatgg
     1861 aagcggttcc tttctcaccc atcaggaaaa ttttcgccga ggtggacaag ctcaaggcag
     1921 agggagtgga catcataacc ctcggcatcg gggagcccga tttcgatacc cccgcgcaca
     1981 ttaccgaggc tatggcggct gcaacccgga acggagcgac ccactatacc gccaacaagg
     2041 gaatcgtcca gctccgggaa gcagtgtgcc ggaagctgaa agtcgaagac ggtctcgaat
     2101 atgaccccga ggaaatcatc tgcaccgttg gtgtttccga aggagtgtac gtttccctga
     2161 gcagctttct ggaccctggg gatgaagttc ttgtcc
//
LOCUS       DAOTHD010000345         2196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_59087_length_2196_cov_2.723494, whole genome shotgun sequence.
ACCESSION   DAOTHD010000345 DAOTHD010000000
VERSION     DAOTHD010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59087_length_2196_cov_2.723494"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="P5219_06570"
     CDS             complement(<1..412)
                     /locus_tag="P5219_06570"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRX26455.1"
                     /translation="MKVPSDIEIAQSAKLRPIVDIAEKLGIGEDDLELYGKYKAKISP
                     EVWERVKNNPDGKLILVTAITPTPAGEGKTTTTVGLSQALAKLGQKVSFAIREPSLGP
                     SFGVKGGAAGGGYSQVIPMEDINLHFTGDLHAITT"
     gene            complement(525..1874)
                     /gene="hydA"
                     /locus_tag="P5219_06575"
     CDS             complement(525..1874)
                     /gene="hydA"
                     /locus_tag="P5219_06575"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HRX26456.1"
                     /translation="MYDLVIRGGTVVTPEAVLAADIGIFGEKIAALGDGLEGKSQVDA
                     SGKLVFPGVIDPHVHFSLPVGGTVSSDDFYSGSVAAACGGVTTVIDFTVGAPERTMAE
                     DLRKRFEDARPSVVDYSFHGEVVGWRPGRRAEIAEAMADGIKSFKFFTAYGASGRRTD
                     TGPLFQAFRAIAEGDGVAVIHAEDDSMIEASAALLSREEWGRMGAVAEVRSDLCEASA
                     VNSVGWIAARTGVRCHIVHLSSALGLGEVETARRRGAVMTAETCPQYLLLTSGVYEGP
                     QGHLYSATPALRTPGDNEALWRGLSSGAVNFAATDHCPFTRSQKEWKGAFDRLPGGLP
                     GVETLLPLLYSEGVLKGRLSLCSLVRATSEQAAKLYGLYPGKGCLLPGSDGDLVIFDP
                     EESWTMRAGALRMNVDFSPYEGMEVRGKVWQTISRGEVIYADGRFLGRRGRGKFLPR"
     gene            complement(1891..>2196)
                     /locus_tag="P5219_06580"
     CDS             complement(1891..>2196)
                     /locus_tag="P5219_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRX26457.1"
                     /translation="GAFYSIGYLGLFSNDMIGGRGWIAFALCFLGNWNPLGALLGAVV
                     FGIADAAAITLQTSGIRMVPNEFLIALPYVLTIAATLARKRFNVPASLGTAYTKEEK"
BASE COUNT          414 a          697 c          644 g          441 t
ORIGIN      
        1 ccgtggtgat ggcatggagg tcgccggtga agtggaggtt gatgtcctcc atgggaatca
       61 cctggctgta cccgcccccg gcagccccgc ccttgacgcc gaagctgggg ccgagggacg
      121 gttcccgtat ggcgaaactc accttctgac ccagcttcgc cagggcctgg gaaagcccca
      181 cggtggtggt ggtctttccc tctccggcag gagtgggggt gatggcggtg acgaggataa
      241 gctttccgtc ggggttgttc ttcacccgct cccacacttc cggtgaaatc ttggccttgt
      301 attttccgta gagttcaagg tcgtcctcgc cgatgccaag tttttccgca atgtcgacta
      361 tagggcggag tttcgcgctc tgggcaatct ctatgtcgct cggtactttc atggtggttc
      421 ctccttcgtc tgtttccctt cgggaaattc tcacaatata cattactcta ccggaaaaca
      481 aatatcaatt gcgttctttt aaatttaccg gaacggcttt catgtcatcg aggaagaaac
      541 tttcccctcc ccctcctgcc gagaaaccgt ccgtcggcat agatgacctc gccccgggag
      601 atggtctgcc agacttttcc ccggacttcc atcccctcgt agggggaaaa atccacgttc
      661 atgcggaggg cgccggccct catggtccag gattcctcgg ggtcgaagat gaccaggtct
      721 ccgtcggagc cgggaaggag acaccctttt cccggataga ggccgtagag tttcgcggcc
      781 tgttcggaag tggcccgcac gagggagcac aaagaaagcc gtcccttgag caccccctcg
      841 gaatagagca gcggaagaag cgtctccacc ccgggcaggc ctccgggcag gcggtcgaag
      901 gctcccttcc actccttctg gcttctggtg aaggggcagt ggtccgtagc ggcgaaattc
      961 accgcaccgg aagaaaggcc gcgccagagc gcttcattgt ctccaggggt ccggagggcc
     1021 ggcgtggcgg aatagagatg cccctggggt ccttcgtaga cgccggatgt gagcagcagg
     1081 tactgggggc atgtctccgc cgtcatgacg gctcctctcc ttcgtgccgt ttcgacttcc
     1141 ccgaggccga gggcggaact gaggtggaca atgtggcacc gaacgcctgt cctggcggcg
     1201 atccatccga cggaattcac cgccgaggct tcgcagaggt ccgagcggac ctccgccacg
     1261 gctcccatcc gcccccactc ctccctggaa agaagggctg cggaagcttc gatcatgctg
     1321 tcgtcttcgg catggataac ggcaaccccg tccccttccg ctatggcccg gaaagcttgg
     1381 aagaggggtc ccgtgtccgt tctccttccc gaggctccgt aggccgtgaa gaacttgaaa
     1441 ctctttatcc cgtcggccat ggcctccgcg atttctgctc tccggcccgg ccgccagcca
     1501 accacctccc cgtggaagga gtaatccacc accgacggac gggcgtcctc gaagcgtttc
     1561 cgcaggtcct ccgccatggt ccgctcggga gcccccacgg tgaaatcgat cacggtggtc
     1621 acgcctccgc aggccgccgc caccgaacct gaatagaaat cgtcgctcga cacggtgccg
     1681 cccacgggaa gggaaaaatg cacatggggg tcgatgacgc cggggaaaac cagttttccc
     1741 gacgcatcca cctgcgactt tccttccagc ccgtctccca gggccgcgat cttttccccg
     1801 aaaatcccga tatcggcggc gagaacggcc tccggcgtga ccactgtccc gcccctgatc
     1861 accaggtcat acattccgtc tctcctccag tcatttttcc tccttggtat aggccgtacc
     1921 cagcgaggcc ggaacgttga accgcttcct ggcgagggtg gccgctatgg taagcacata
     1981 gggaagggcg atgagaaact cgttgggaac catgcggatg cctgatgtct ggagggttat
     2041 ggccgcggcg tcggctatgc cgaagaccac cgctcccagc agagcaccca ggggattcca
     2101 gtttccgaga aaacagaggg caaaggcaat ccatccccgc cctccgatca tgtcattgga
     2161 aaaaaggccg aggtagccga tggagtagaa tgctcc
//
LOCUS       DAOTHD010000346         2195 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_59102_length_2195_cov_3.787850, whole genome shotgun sequence.
ACCESSION   DAOTHD010000346 DAOTHD010000000
VERSION     DAOTHD010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59102_length_2195_cov_3.787850"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="P5219_06585"
     CDS             complement(<1..805)
                     /locus_tag="P5219_06585"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HRX26458.1"
                     /translation="MKKKMTYLFCAVLMLGIALAGFRPNAAEAAGGAVKIALIIESTV
                     DDKGWCQAMHDGILQAQKELPGRIEYSYSEKMKPVDAGSAVLQYVSQGYHIIIGHGAQ
                     YKNLMLEMAEEYPDVTFAFGTSAEIGPENVFTYMPQSEETGYLSGLIAGMTTKKNTVG
                     LVGPVDGGDAARYNRGFVLGVQAVNPKAKIMVAHTGSFADFVKAGEVAETQIKAGADV
                     LTGSSQQALGALRAVADHRDAPIWWVGQDIAQIRITEGYKCIAASSYNYG"
     gene            1143..1703
                     /locus_tag="P5219_06590"
     CDS             1143..1703
                     /locus_tag="P5219_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26459.1"
                     /translation="MTARAASSVKRSESPEDLRREAARDPLGLMKRIGCRAILFMKDE
                     QRSITLKWAFRVFCEFRETFRVHGAPVESSTFLPHPRKDIEAALFILLFQAVLAGEKE
                     KAAVFRKAMRSLARFTDIAEGDGDLVDLALRVAADPAMMESAEKATKERCGEALVTLE
                     RYVDAVRGEEERLSRKERDLLGIYGQ"
     gene            1841..>2195
                     /locus_tag="P5219_06595"
     CDS             1841..>2195
                     /locus_tag="P5219_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HRX26460.1"
                     /translation="MTQWRSLAERMVREQIMARGVRSEAVLQAMRTVPRHLFVDEALR
                     ESAWIDSPLPIGEDQTISQPYMVARMTELLNPARGETVLEIGTGSGYQAAVLCAMGAK
                     VTSLERIASLADRARE"
BASE COUNT          405 a          594 c          711 g          485 t
ORIGIN      
        1 ccccgtagtt gtaggaggag gcggcgatgc acttgtatcc ctcggtgatg cggatctggg
       61 cgatatcctg gccgacccac cagatcgggg cgtctctgtg gtccgccacg gcccgcagtg
      121 cgccgagagc ctgctgggaa gagccggtga gcacgtctgc cccggccttg atctgcgttt
      181 cggccacttc tccggccttg acgaaatcag cgaaggaacc ggtgtgggcg accatgatct
      241 tcgccttcgg attgaccgcc tgcacgccca gcacgaagcc ccggttgtag cgggcggcat
      301 ctccgccgtc cacgggaccg acaaggccaa cagtgttctt tttggtggtc atgcctgcga
      361 tgaggccgga gaggtagcct gtctcctcgc tctggggcat gtaggtgaag acgttttcgg
      421 gtccgatctc ggcgctggtg ccgaaggcga aggtgacgtc cgggtactcc tcggccattt
      481 cgagcatcag gttcttgtac tgggcgccgt ggccgatgat gatgtggtat ccctgggata
      541 cgtactggag tacggcggat ccggcgtcca cgggcttcat tttttcgctg tagctgtact
      601 ctatccgtcc gggaagctct ttctgcgcct ggagaatgcc gtcgtgcatg gcctggcacc
      661 atcccttgtc atcgacggtg ctttcgatga tgagggcaat cttcactgca ccgccggcgg
      721 cttcagcggc gtttggccta aaacccgcaa gggcaatccc gagcatcagg acggcgcaga
      781 acagatatgt catttttttc ttcacaagaa acccctccct gtttttcaaa ttttttatag
      841 cccgtgaatg tgcatccagt gtaagaactg cctgaagatt gtcaagctgt ttcgggcggc
      901 ggtgttccac cgggaggggc aataaacttg ctttttgctt ttcagactgc tacactgaat
      961 ttacgtagag tattgaaaat acagtattcg cgaagatcga aaggtaagct gaacgggggg
     1021 gctttccctc aagaccttca ggcttatcgg cttggtgccg tcggaatgac ggtgtttctc
     1081 ggctttacac ttccggtgtc gtccggagat ctccagggtg tacagacagg aggaatggaa
     1141 ggatgactgc ccgtgcagct tcgtcggtga agaggagcga gagcccggag gacctgaggc
     1201 gcgaagccgc aagggatccc ctggggctga tgaagaggat cggctgtagg gccatcctgt
     1261 tcatgaagga tgagcagcgg tccattacgc tgaaatgggc attcagggta ttctgcgagt
     1321 tccgggagac cttccgtgtt catggggcgc cggtggaaag cagcacattt cttcctcatc
     1381 cccggaagga cattgaggcg gctcttttca ttcttctgtt tcaggctgtc ctggcaggag
     1441 aaaaggagaa ggctgccgtc ttccgcaaag ccatgcgctc actggcccgg tttacggata
     1501 ttgcggaagg ggacggggac ttggtggatc ttgcgctgag ggtcgccgcc gatccggcca
     1561 tgatggagtc tgcggagaaa gcgacgaagg agcgctgcgg cgaggccctc gtgacccttg
     1621 agcggtatgt tgatgccgtg cgcggcgagg aagagcggct ctcccgaaaa gaaagggacc
     1681 tcctgggaat ctatggccag tgataccgcc cggtgccaat gttttttccg tgtttccgtc
     1741 cgcccctgcg ggggcggttt ttttgcgccc ccaccgggtc cggcgtgttc gtcatggtac
     1801 aatactccgg taaacatctg ctggagtgga gggattttcc atgacccagt ggcggagtct
     1861 ggcggaacgg atggtccggg agcagatcat ggcaaggggc gtgcgctccg aagcggttct
     1921 ccaggccatg cggacagtgc ccaggcacct ttttgtggat gaggcgctcc gggagagcgc
     1981 ctggatcgac agcccccttc caatcggcga ggatcagacc atttcccagc cctacatggt
     2041 ggcccggatg acggaacttc tgaatcccgc ccggggagaa acggtccttg agatcggcac
     2101 cggttcgggg taccaggcgg cggtcctctg tgctatgggt gcgaaggtga caagcctgga
     2161 acggatcgcc tccctggcgg acagggcccg ggaac
//
LOCUS       DAOTHD010000347         2191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_59247_length_2191_cov_2.713951, whole genome shotgun sequence.
ACCESSION   DAOTHD010000347 DAOTHD010000000
VERSION     DAOTHD010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59247_length_2191_cov_2.713951"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="P5219_06600"
     CDS             <1..1111
                     /locus_tag="P5219_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRX26461.1"
                     /translation="ADEGYIYCRAEYPLAIKRLQTAIHQATEYGLLGANVLGTDFSFD
                     LHIKEGAGAFVCGEETALMASIEGQRGMPRPRPPFPAVKGLWGKPTNINNVETWANVP
                     RIMLQGAECFAAMGTEKSKGTKVFALTGKVKNTGLVEVPMGITIREIVYEIGGGIIGD
                     KKFKAVQIGGPSGGCLVEEHLDLPVDYESLTAAGAIMGSGGLVVLDESNCMVDTAKFF
                     LEFTQRESCGKCVPCREGTKKMLDILTRITEGNGKPEDLDTLQYLGEQIKATSLCALG
                     GTAPNPVLTTLKYFRHEYEAHINEKRCPAGACQSLLRYTIDPEKCIGCTKCARNCPVS
                     CISGKVKEPHVIDQEKCIKCGACMANCPVGAISKK"
     gene            1134..>2191
                     /locus_tag="P5219_06605"
     CDS             1134..>2191
                     /locus_tag="P5219_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRX26462.1"
                     /translation="MDGIQAIIDGKEITAEPGMTILQAAKANGIHIPTLCDHPAVSPF
                     GACRICLVEAEKNPKLLPSCTTPITSGMVVHTNTPKVIKARKAVLELILVRHPLNCFS
                     CPSNGKCELQDVAYELGVKESSYADPGDTNTSHEYEDANPFYVRDLNKCILCGRCVRV
                     CEEQAQYHAIDFQGRGIRTSIQPPVGSPIELSDCVFCGQCVQVCPVGALSEKTALGMG
                     RPWDIEKVKTVCSYCGVGCEVEINVNRKTGKIANVTTNYSSPTALNKGRSCVKGRFAW
                     QFVNSEDRLTTPLIREGDTFRKASWDEALSLVAEKLGSIKEKHGGDALGFFSSARCTN
                     EENYLMQRLAREVMGTNNV"
BASE COUNT          535 a          641 c          622 g          393 t
ORIGIN      
        1 agccgacgaa ggatatattt actgccgcgc ggaatatccc ctggccatca agagactcca
       61 gacggccata caccaggcca ccgaatacgg cctcctgggg gcaaacgtcc tggggacgga
      121 cttcagcttc gacctgcata tcaaggaagg cgcaggtgcc tttgtgtgcg gcgaggaaac
      181 ggctctcatg gcatccatcg agggacagag gggaatgccc cggccccggc caccgttccc
      241 ggcggtcaag ggtctctggg gcaagcccac gaacatcaac aacgtggaga catgggcaaa
      301 cgtgccgcgg atcatgctcc agggtgccga atgcttcgca gccatgggga cggaaaagtc
      361 caagggcaca aaggtgttcg ccctcacggg gaaagtcaaa aacaccggtc ttgttgaagt
      421 tcccatgggt atcacaataa gggaaatcgt ctacgagatc ggcggcggca tcatcggcga
      481 caagaaattc aaggcggttc agatcggcgg cccctccgga ggctgtctcg tggaggagca
      541 cctcgacctt cccgtggatt acgaatctct cacggcggcg ggagccatca tgggttccgg
      601 cggtcttgtc gtcctcgacg aatcgaactg catggtggac accgcgaaat tctttcttga
      661 gttcacccaa agggaatcct gcggaaaatg cgttccctgc cgggaaggga cgaagaagat
      721 gctcgatatc ctgaccagga tcaccgaggg caacggaaaa ccggaagacc tcgataccct
      781 gcagtatctc ggcgaacaga tcaaggcgac atccctctgc gccctgggcg gaacagctcc
      841 aaacccggtg ctgacgacgc tgaaatactt ccggcacgag tacgaggctc acatcaacga
      901 aaaacggtgc cctgccggag cgtgccagag ccttctccgc tataccatcg acccggaaaa
      961 gtgcatcgga tgcaccaagt gcgccaggaa ctgccctgtg agctgcattt ccggcaaggt
     1021 gaaggaaccc cacgtcatcg atcaggaaaa atgcatcaag tgcggcgcat gcatggcgaa
     1081 ttgcccggtc ggcgcaatat ccaaaaagta atcacgaagg agacgatcag agaatggacg
     1141 gcattcaagc gatcatcgac ggaaaggaga taactgcgga acccggtatg accattttgc
     1201 aggctgcaaa ggcgaatggc atccatatac cgactctgtg cgatcatcct gcggtttctc
     1261 ctttcggcgc atgcagaatc tgcctcgtcg aggcagaaaa gaaccccaag cttcttcctt
     1321 cctgcactac accgatcact tccggcatgg tggtccatac gaacaccccg aaagtaatta
     1381 aagcccggaa ggccgtgctg gagcttattc tggtgcgcca tcccctgaac tgtttctcct
     1441 gcccgagcaa cggcaagtgt gaactccagg acgtggccta cgaactggga gtcaaggaat
     1501 ccagctatgc tgatcccggt gacacgaata cctctcacga gtacgaagat gccaatccct
     1561 tctatgtccg cgacctgaac aagtgcattc tgtgcggccg gtgtgtccgc gtatgcgaag
     1621 agcaggccca gtaccacgct attgatttcc agggacgggg catcaggaca tcaatccagc
     1681 ccccggtcgg atctcccata gaactgtccg actgtgtgtt ctgcggccag tgcgttcagg
     1741 tctgtccggt cggcgccctc tccgagaaga ctgctctcgg catgggccgc ccctgggaca
     1801 ttgaaaaggt caagaccgtc tgctcctatt gcggagtagg ctgcgaagtg gaaatcaacg
     1861 tcaacaggaa aaccggcaaa atagccaacg tcacgacgaa ctactccagc cccacggccc
     1921 tgaacaaagg aagatcctgc gtcaagggac ggtttgcctg gcagttcgtc aacagcgagg
     1981 acaggcttac gacacccctt atccgtgagg gagacacctt ccggaaggcc tcctgggacg
     2041 aagccctctc cctggtggcg gaaaagctcg gcagcataaa ggagaagcac ggcggagacg
     2101 ctctcggctt cttctcttcc gcccggtgca ccaacgagga gaactatctc atgcagcgcc
     2161 tcgcaaggga ggtgatgggc accaacaacg t
//
LOCUS       DAOTHD010000348         2186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_59435_length_2186_cov_2.895824, whole genome shotgun sequence.
ACCESSION   DAOTHD010000348 DAOTHD010000000
VERSION     DAOTHD010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59435_length_2186_cov_2.895824"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="P5219_06610"
     CDS             <1..756
                     /locus_tag="P5219_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRX26463.1"
                     /translation="WEKSDGVGSITLNRPEAMNTFNTELAAELNDAFRTMESDDAVRA
                     VVLSGAGKHFSTGIDLREYLTKERHEIRDFLSLMNLHNHTLAAMTKPVIASVQGYALA
                     NGTGLALACDFVVASEDAVFGTTAVNVGLICLEPGYQLGRWIGEKRALQYVLTGDLIP
                     ASKGKELGFVYAVTEKEKLEENSLALAARLASKSRLSVRTGKRGFAAMSGLPVERAVE
                     AGGELFAALACSEDGREGVEAFLAKRPAVFRGK"
     gene            777..1100
                     /locus_tag="P5219_06615"
     CDS             777..1100
                     /locus_tag="P5219_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003359266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HRX26464.1"
                     /translation="MIITTTHTIEGKQITSYRGIVFGEVIAGANFMKDFKASLTNFFG
                     GRSGAYEKELTEAREAALKEMEQRAAELGANAIVGMLVHYEVLGSNGNNMLMVSTCGT
                     AVRTD"
     gene            complement(1136..>2186)
                     /locus_tag="P5219_06620"
     CDS             complement(1136..>2186)
                     /locus_tag="P5219_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HRX26465.1"
                     /translation="DLLREIFALPGVREDVLPSILETERPCVTAQTLEVLREYLLGLS
                     PRDMVDRLFFGMTKDELNRETGEVSLHDLADRRDEWCLFPMTNVLYSRDSAAVLGEGV
                     VFGRMLNRDRMKEPACVKALFKHHPLLKDTGFKTWYGAAEGDDRPVEGGNIHCYSPNV
                     FVVGVNERTHPESIERIARRTMESGHITDVLALLFENPRLGPQDSIGLTVHVDMFLNM
                     IDHDAFLFFPYIEKKISVLHITRGRGDRLRITRENSLFGAIRKVLKLSSLRIVKVGGS
                     ESEAVAFAEQRAGSGGNTFTLEPGRVCIWDRNTGTIRALEKAGIDVVPVEADELVKGG
                     GGPRCSTMPLWRDDL"
BASE COUNT          470 a          667 c          638 g          411 t
ORIGIN      
        1 tgggaaaaaa gcgatggtgt aggctccatc accctcaacc gccccgaggc catgaacacc
       61 ttcaatacgg aactcgcagc cgaactgaac gatgccttcc gcaccatgga gtccgacgac
      121 gccgtgcggg cagtcgtcct ctccggtgcg gggaaacatt tctccacggg aatcgacctc
      181 agggagtacc tgacaaagga acggcatgag atcagggatt ttctctcgct catgaacctc
      241 cacaaccaca cccttgcggc catgacgaag cccgtcatcg cctcggttca gggatacgcc
      301 ctggctaacg gaacgggact tgcccttgcc tgcgacttcg tcgtggcatc ggaagacgcc
      361 gtcttcggca ccacggcggt gaacgtcggt ctcatctgcc tggagcccgg ataccagctt
      421 ggccgatgga taggtgaaaa acgggccctt cagtatgttc tcacagggga ccttataccg
      481 gcttccaaag ggaaggaact cggattcgtc tatgcggtga cggaaaaaga aaaactcgag
      541 gaaaactccc ttgccctggc ggcacggctg gcctcgaaga gcaggctttc ggtccgtacg
      601 ggaaaaaggg gcttcgccgc catgtccggt ctccccgttg aacgggccgt ggaggcggga
      661 ggggaactgt tcgccgccct tgcgtgctcc gaagacggaa gggaaggcgt ggaggccttc
      721 ctcgccaagc gccccgcagt gttcagggga aaatagaaga aaggggattc cagcagatga
      781 tcattaccac cacccacacc atcgaaggca aacagatcac gtcctatcgg gggatcgtct
      841 tcggggaagt catcgccggg gccaacttca tgaaagactt caaggcgagc ctcacaaatt
      901 tcttcggcgg ccggtcggga gcctatgaaa aagaactcac cgaggccagg gaagccgccc
      961 tgaaggaaat ggaacagcgg gctgccgagc tgggggcgaa cgccatcgtg ggcatgctgg
     1021 tccattacga ggtgctgggc agcaacggaa acaatatgct catggtctcc acctgcggaa
     1081 cggcggtccg gacggactga ccaggaacag attcgacgcc gcggacggtt agctcctaga
     1141 ggtcgtccct ccacagcggc atggtggagc accggggacc tcccccgccc ttcaccagct
     1201 cgtcggcttc cacaggcacc acgtcgatgc cggccttttc cagggcccgg atggttcccg
     1261 tattcctgtc ccagatgcac accctgccgg gctccagcgt gaaggtattt ccgccggagc
     1321 ccgctctctg ttcggcgaag gccaccgctt cgctctcgct gccccccact ttgacgatcc
     1381 ggagggacga cagcttcagc actttccgga tggccccgaa aagagagttt tccctggtga
     1441 tcctgagccg gtcaccccgt ccccgggtaa tgtgaagcac agagattttc ttttcaatat
     1501 aggggaaaaa gagaaaggca tcatggtcga tcatgttcag gaacatgtca acatggacag
     1561 taagcccgat ggaatcctgg ggaccgagcc tcggattctc gaaaaggagg gccagcacgt
     1621 cggtaatgtg ccccgattcc atcgtcctgc gggcgattcg ctcgatggac tcggggtggg
     1681 tcctttcgtt cacgccgaca acgaagacgt tcggactgta gcagtggatg ttgcctccct
     1741 caacgggcct gtcgtcacct tcggcggcgc cgtaccatgt tttgaacccc gtgtccttca
     1801 gcagcgggtg atgtttgaaa agagccttca cgcaggcagg ctccttcatc ctgtctctgt
     1861 tcagcatcct gccgaaaacc accccttccc cgagaacagc cgcggaatcc cgggaataga
     1921 gcacgttcgt catggggaaa aggcaccatt cgtccctcct gtcggccagg tcatgcaggg
     1981 agacctcccc ggtctccctg ttgagttcat ccttcgtcat gccgaaaaaa agccggtcca
     2041 ccatgtcccg gggggacagc cccagaaggt attcccggag cacttccagt gtctgggccg
     2101 tgacacaggg ccgttcagtc tccagtatgg aaggcaggac atcttccctc acccccggaa
     2161 gggcgaaaat ttcccggagc aggtct
//
LOCUS       DAOTHD010000349         2185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_59471_length_2185_cov_3.350704, whole genome shotgun sequence.
ACCESSION   DAOTHD010000349 DAOTHD010000000
VERSION     DAOTHD010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59471_length_2185_cov_3.350704"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /locus_tag="P5219_06625"
     CDS             <1..1266
                     /locus_tag="P5219_06625"
                     /inference="COORDINATES: protein motif:HMM:NF001989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HRX26466.1"
                     /translation="NPVLLKPEGHSRSQVILMGRPWKTLSAGDYYACRETLWASVAAS
                     FEKLAGENDLLLVEGAGSPAEINLKKSEIVNMRVARTFGCPVLLAGDIDRGGVFASLT
                     GTLALLDDEEKTLVKGFLINKFRGDVKLLEPGLEMLSGLTGGRPVLGVIPWLKNLAVA
                     QEDSVYLEENRSSGIPGAVDIVVIKFPRISNYDDFDPLALEEGVSLRFVDRPEDLGKP
                     HAVILPGSKTTLEDLAWLRRSGFDGLLAALVLSGASLAGVCGGYQMLGRRISDPLGVE
                     GASGGGDGLGFLPGETLFEPSKETTRVTGATVPGEGFPGSRSVPVEGYEIHMGRTVLP
                     RGARPLLSLGGGRTDGAVSAGGRIWGTYLHGIFDSGEFREEWLRFLGRREPGKDLPFR
                     VVREQAFDRLADEVEAALDMKALERIIGL"
     gene            1263..2153
                     /locus_tag="P5219_06630"
     CDS             1263..2153
                     /locus_tag="P5219_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26467.1"
                     /translation="MTTVAAACPGTCGELFQGTLEGIPCLVSCPIDRYARIRITARPG
                     EGLSLPGEMSKTRRALEAFLGRRFLGGKNLAVERMEALPEGKGYASSTADILSALACA
                     AFLAGTSLPPEEASAIALSVEPTDSIAWPGLALLDHREGRIMRFLGPPPPLSVFVLDW
                     GGTVDTEEFNSRNSRPVLESLAPLHREAFSLVLRGVEQGDPEALGRGASLSARASARL
                     LEKPRLDECFALCSLLGGYGVCVAHSGTLYGILLPSGAAGREDEILGTAARRLSPGWE
                     GRMHSVVSGGPGREERKERP"
BASE COUNT          352 a          704 c          739 g          390 t
ORIGIN      
        1 aaccccgtcc ttctgaagcc ggagggacat tcacgctccc aggtcatcct catgggacgg
       61 ccgtggaaaa ctctctctgc cggggattat tacgcctgca gggagaccct gtgggcttcg
      121 gtggccgctt ccttcgaaaa gctcgccggg gagaacgacc tccttctcgt ggagggggcc
      181 ggcagcccgg cggagatcaa cctcaaaaag agcgagatcg tgaacatgcg ggtggcccgg
      241 accttcggct gtccggtgct cctcgccggt gacatcgaca gaggcggtgt tttcgcctcc
      301 ctcacgggga ccctggccct cctggacgac gaggaaaaga cccttgtgaa gggcttcctc
      361 atcaacaagt tccgggggga tgtgaaactc ctcgaaccgg ggctcgaaat gctctccggc
      421 ctgacggggg ggcgccctgt ccttggggtg atcccctggc tgaaaaatct cgccgtggcc
      481 caggaggatt cggtctacct cgaggagaac cggtcctccg gtatccccgg agcagtggac
      541 atcgtcgtga tcaagttccc gcgcatttcc aactacgacg acttcgaccc cctcgccctc
      601 gaggaaggtg tctccctccg gttcgtggac aggcccgaag atcttggaaa accccatgcc
      661 gtcatcctcc ccggaagcaa gacgacgctg gaggacctcg catggctccg ccggtcgggt
      721 ttcgacggtc tccttgccgc ccttgtcctg tcgggggcat ctctcgccgg cgtctgcggg
      781 ggctaccaga tgctcggccg ccggatttcc gaccccctgg gggtggaggg agcctcaggc
      841 ggcggggacg ggctcggctt cctccccggc gagacccttt tcgaaccctc caaggagact
      901 accagggtaa cgggcgccac cgttcccgga gaggggttcc cgggctcccg gtccgttccc
      961 gtggaagggt acgagatcca catgggcagg acggtcctcc cccggggggc caggccgctc
     1021 ctgtccctgg ggggcggaag gaccgacggc gccgtgagcg ccggggggcg gatatgggga
     1081 acctacctcc acggcatctt cgacagcggg gagttccggg aggaatggct ccgctttctc
     1141 ggcaggaggg aaccggggaa ggaccttccc ttccgggtgg tccgggagca ggccttcgac
     1201 cgtctcgccg acgaggtgga ggctgccctc gacatgaagg cgctggagcg gatcatcggc
     1261 ctatgaccac tgttgccgct gcctgcccgg gaacctgcgg ggagcttttc cagggcaccc
     1321 ttgaaggcat accctgcctt gtgtcgtgtc ccatcgacag gtacgcccgg atccgtatca
     1381 ccgcccgccc gggagaaggg ctctctcttc ccggggagat gagcaaaacc agaagggccc
     1441 tggaagcctt cctgggacgc cggttccttg gggggaagaa ccttgcggtg gagcggatgg
     1501 aagctctgcc tgaaggcaag ggatacgcaa gcagtaccgc cgacattctt tcggccctcg
     1561 cctgcgcggc gttcctggcg gggacttccc tgccgccgga agaagcgtct gcaatcgccc
     1621 tttctgtgga gcccaccgac agcatcgcct ggccgggact ggccctcctc gaccatcggg
     1681 aggggaggat catgaggttc ctcgggcctc ccccgcctct gtctgtgttc gtcctcgact
     1741 gggggggcac cgtggacacc gaagaattca acagcagaaa cagccgtcct gtcctggaat
     1801 ccctcgcacc ccttcaccgg gaggccttct ccctggtcct ccggggggtg gagcagggag
     1861 acccggaagc cctgggcagg ggggcgtccc tgagcgcccg ggcgtcagcc cggctcctgg
     1921 aaaagccccg cctcgacgag tgcttcgctc tctgctcatt gctggggggg tatggggtct
     1981 gcgtggccca cagcggtacc ctctacggca tcctcctgcc ctccggggcg gcgggccggg
     2041 aggatgaaat cctgggaacg gcagcccgga ggctttctcc cgggtgggag ggacggatgc
     2101 attccgtcgt ttccggcggg ccgggaaggg aagaaaggaa ggaacggcca tgatgcttgt
     2161 ttttattctc ctgggggcca cggtg
//
LOCUS       DAOTHD010000350         2185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_59474_length_2185_cov_3.158216, whole genome shotgun sequence.
ACCESSION   DAOTHD010000350 DAOTHD010000000
VERSION     DAOTHD010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59474_length_2185_cov_3.158216"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="P5219_06635"
     CDS             complement(<1..249)
                     /locus_tag="P5219_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRX26468.1"
                     /translation="MGERERTEARILDAVGAVLSGKGYGYLGVNEVARQAGVDKVLIY
                     RYFGGMPQLLNAFFSGRKYWPSTEEILQTGGEGGADPAT"
     gene            complement(313..1971)
                     /gene="ilvD"
                     /locus_tag="P5219_06640"
     CDS             complement(313..1971)
                     /gene="ilvD"
                     /locus_tag="P5219_06640"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRX26469.1"
                     /translation="MRSDKAKAGPERAPHRSLLKASGYTDWEISRPWIGVVNPYNAII
                     PGHVHLNRITEGVKSAVYAFGGLPLEFPVIGVCDGIAMNHEGMKFSLPSRELIMDSIE
                     VMVRAHALDGLVLVTNCDKIIPGMAMAALSLNIPAVMISGGPMLAGTSGGRDVDLSNI
                     FEAVGKRVAGLMTDDELRAIEDTTCPTCGSCAGMFTANTMNCMVEALGLALPGNGTIP
                     AVYSARDRLAKEAGRHIMMLVEKNIRPRDILTEKAFKNAVAVDMALGGSTNTALHLPA
                     LAHSAGISLTLDHFEEASRKTPHICSMSPGGNHHIQDLFSAGGVQAVMARLLGGDLID
                     GSPLTVTGRTVEENLRGVRVLDDDVIRPLENPYHAQGGLAILKGNLAPEGSVVKQSAV
                     DPSMLKHSGPARVFDSEEAASEAILAKKINHGDVVVIRYEGPKGGPGMREMLGPTSSL
                     AGMGMDKTVALITDGRFSGASRGASIGHVSPEAAAGGNIALVQEGDTIEIDIPARSMH
                     LAVSDEELGKRRAAWKPAESVTDSLFLERYRSVVTSGSRGAVFG"
     gene            complement(2007..2180)
                     /locus_tag="P5219_06645"
     CDS             complement(2007..2180)
                     /locus_tag="P5219_06645"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26470.1"
                     /translation="MAIDIYVASAITGLPIEQISKPIVPMVAVLIGVLVLVTYVPSII
                     LSLPLLVWGPAGI"
BASE COUNT          406 a          671 c          662 g          446 t
ORIGIN      
        1 ggtcgctggg tcggcccctc cttcgccgcc ggtctggagt atctcctccg tagagggcca
       61 gtatttcctc ccggaaaaaa aagcgttcag aagctggggc atgcctccga aatagcggta
      121 aataagcacc ttgtccactc cggcctgccg ggctacctcg tttacgccga gatagccgta
      181 tcccttgccg gaaaggacgg ccccgacagc gtcaagaatg cgggcttccg ttctctccct
      241 ttctcccaca acgctccctc ccctgaaaaa gaaggggccc ggaatcgggg ccccttcggc
      301 agcagacaga ttctagccga aaacagcccc cctggagccc gaggtgacaa cgctccggta
      361 tctttcaagg aacaggctgt ccgtaacgct ttcagcgggt ttccatgcgg cccgtctctt
      421 cccgagctct tcgtcggaca ccgcgagatg catcgaccgg gctgggatat cgatttcgat
      481 ggtatcgccc tcctgcacga gggcgatgtt tccgccggca gcggcttcag gggatacgtg
      541 accgatggag gctcccctcg acgcccccga aaaacggccg tcggtgatca gggctacggt
      601 cttgtccatt cccatccccg ccagggagga agtgggcccg agcatctccc gcattcccgg
      661 tccgcccttc ggtccctcgt accgtataac caccacgtct ccgtggttga ttttcttagc
      721 aaggatggcc tcgcttgccg cttcctcgct gtcgaatacc cgggcagggc ctgagtgctt
      781 cagcatggag ggatccacgg cggactgctt cactacgctg ccttcgggag cgaggttgcc
      841 cttcaggatg gccaggcctc cctgggcatg ataggggttc tcgagggggc ggatgacatc
      901 gtcgtcaagt acccggactc cccggaggtt ttcctcgacg gtccgtccgg tgacggtaag
      961 aggcgaacca tcaatcaggt ctcctccaag gagccgggcc atgacagcct ggactccgcc
     1021 cgctgagaaa agatcctgga tgtggtggtt cccccccgga ctcatgctgc agatatgggg
     1081 cgtcttcctg ctcgcttcct cgaaatggtc gagggtgagc gatatccctg ccgaatgggc
     1141 gagggccgga aggtgaaggg ccgtgttggt ggaccccccg agggccatgt ccacggccac
     1201 ggcgtttttg aaagcctttt cggtgaggat gtccctcggc cgtatattct tctccaccag
     1261 catcatgatg tgccgtcccg cttccttggc gagccggtcc cgtgccgaat aaacggccgg
     1321 gatcgtgccg ttgcccggca gagccaggcc gagggcctcg accatgcagt tcatggtgtt
     1381 cgcggtaaac atgcccgcac aggagccgca ggtggggcag gtggtatcct caatggcccg
     1441 cagctcgtca tcggtcatca gtccggcgac ccttttgcct accgcctcga agatgttgct
     1501 gaggtcaacg tcacgtccgc cgctggtccc tgcgagcatg ggacctccgc tgatcatgac
     1561 ggcggggatg ttcagggaaa gggctgccat agccataccg gggatgatct tgtcgcagtt
     1621 ggtgacgaga accagaccat cgagggcgtg cgccctgacc ataacttcga tggaatccat
     1681 gatgagttcc ctgctgggaa gggaaaactt catgccctcg tggttcatgg cgatgccgtc
     1741 acagaccccg atcacgggaa actcgagcgg gaggcccccg aaggcgtaga cagccgactt
     1801 cactccctcg gtgatcctgt tcaggtgaac gtggccgggg ataatggcgt tgtagggatt
     1861 gaccacacca atccatggac ggctgatttc ccagtcggta tatcccgaag ccttcagcag
     1921 ggaccggtga ggagcccttt ccggacccgc tttcgctttg tcgctccgca taatgcatta
     1981 cctcctgtcg attattgagt tgtttgtcag attccggccg gtccccagac gagcagcggc
     2041 agtgacagga tgatggaggg aacgtaggtc acaaggacca ggactccaat aagcaccgcc
     2101 accatgggaa cgatgggttt gctgatctgc tcgatgggca ggccggtgat ggcgctggcg
     2161 acataaatat cgatggccac cggcg
//
LOCUS       DAOTHD010000351         2175 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_59841_length_2175_cov_3.304245, whole genome shotgun sequence.
ACCESSION   DAOTHD010000351 DAOTHD010000000
VERSION     DAOTHD010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59841_length_2175_cov_3.304245"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="P5219_06650"
     CDS             <1..1312
                     /locus_tag="P5219_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX26471.1"
                     /translation="PRSGGGSVFWCELPFGRVPFGEAEDLPVDQRLLGFPVILAGGGA
                     SWQTEASGLAGKWLCSVRTADSPEDLRSLLEKRSGGDGDGRCAVFVDRELLRKEAGWS
                     PSALPADLRRGVLFFSVDPPGTFPETGYLLREGLAGRITSPLRRGVFREAVLSAAETD
                     RGKRQGSPGGSEPAGSFRSALLPARVLIADDSRVNRKVAARMLQKLGCSVTGAADGEE
                     ALRMLQTERFDIVLMDVQMPGKDGLEVTSALRSPEWTGCNGSVPVIAMTAHALPGDRE
                     KCLAAGMDDYLPKPVRFRDLEEKLSVHLSRRRPEEIDAPAEGTGGVYCREEAVLRLNI
                     DGEELDALAGEYLEDALEILDRMADAEERGDEKAAADAAHTLKGMSANIGAERVRAAA
                     LRAERSWREGGNGASMADVLRAELRNFGNVLGWVRERRDLREDR"
     gene            1299..1823
                     /locus_tag="P5219_06655"
     CDS             1299..1823
                     /locus_tag="P5219_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HRX26472.1"
                     /translation="MKTDERRRMITAELSGAGEAVTGSDLASRFGVSRQVIVQDMAVL
                     RAEGLPVVATPRGYILLRPEGSGRLVKTVVTRHRDYEAMEEELLIMVGCGAKVLNVIV
                     EHPLYGEITGNLMLSTPADVARFMESIRKSAGEPLSSLTGGVHLHTLEVDGEEGWERL
                     LAELGEKNYLPEGR"
     gene            1872..>2175
                     /locus_tag="P5219_06660"
     CDS             1872..>2175
                     /locus_tag="P5219_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HRX26473.1"
                     /translation="MAGTASSSPLRGYLIKVSNGMALGLFSSLIIGLILRQCGQLLSL
                     PHLEHFGRVAQLLMGPAIGAGVAFAVGAPPLGVFSSLVAGAVGAGTFSGSAAAVGEP"
BASE COUNT          383 a          639 c          765 g          388 t
ORIGIN      
        1 cccccgcagc ggaggcggat cagttttctg gtgcgaactg cccttcggca gggtgccctt
       61 cggcgaggcg gaagacctcc ccgtggacca gcgcctcctg gggtttcccg tgattcttgc
      121 ggggggcggg gcctcctggc agactgaagc gtcaggcctg gcagggaagt ggctctgttc
      181 cgtccggacc gcggacagcc cggaggatct caggagcctc ctcgagaagc ggtcaggggg
      241 tgacggtgat gggcggtgcg ccgttttcgt ggaccgggag cttctgagaa aggaagcggg
      301 ctggtcaccc tcagcccttc ctgcggacct ccggaggggc gtcctgtttt tctccgtgga
      361 cccccccggg acatttccgg agacgggata tcttctccgg gagggccttg ccggccggat
      421 aacaagcccg ctgcggcggg gagtctttcg tgaagccgtc ctttcggcgg cggagactga
      481 ccgggggaag cggcagggtt cacccggggg ctctgaaccg gcgggaagtt tccggagcgc
      541 ccttctcccg gccagggttc tgatcgccga tgacagccgg gtgaacagaa aggtggcggc
      601 gcgaatgctg cagaagctcg gctgttccgt caccggggcg gcggacggtg aagaagccct
      661 ccgcatgctg caaacagaga gattcgacat tgttctcatg gatgtccaga tgccgggaaa
      721 ggatggcctt gaagtcacct ccgcccttcg gagcccggaa tggaccggat gcaacggatc
      781 cgttcccgtg atcgccatga cggcccatgc cctgccggga gaccgggaga agtgtctggc
      841 cgccggaatg gacgattatc ttccgaagcc ggtcaggttc agggacctcg aagaaaaact
      901 ctcggttcac ctgtcccggc gcaggccgga ggaaatcgac gccccggcgg aaggaaccgg
      961 cggggtatac tgcagggaag aggctgtcct gaggctcaac attgacgggg aggaactcga
     1021 cgctcttgcg ggggaatacc tcgaggatgc ccttgagatt cttgaccgga tggcagacgc
     1081 ggaagagcgg ggggacgaaa aggcggcggc tgatgctgcc cacacgctga aggggatgtc
     1141 ggccaatatc ggggcggaaa gagtccgggc agcggccctg agagcggaga gatcatggcg
     1201 ggagggcggc aacggcgcct ctatggcgga tgtcctccgg gcggaactcc ggaacttcgg
     1261 aaatgttctc ggctgggtcc gggaaaggag ggacctgcgt gaagaccgat gagcggaggc
     1321 ggatgatcac cgcagaactc tccggggcgg gagaggcggt cacgggaagt gacctggcct
     1381 cccggttcgg cgtcagcagg caggtgattg tccaggacat ggcggtgctc cgggctgaag
     1441 gccttcccgt ggtggccacc ccacggggct acatcctgct gcgcccggaa ggatccggca
     1501 ggctcgtgaa gacggtggtc accaggcaca gggactacga agccatggag gaggagctcc
     1561 tgatcatggt gggctgcgga gcgaaagtgc tcaacgtcat cgtggagcat cctctctacg
     1621 gagaaatcac cggcaatctc atgctctcca ctcccgccga cgtcgcccgt ttcatggagt
     1681 ccatcagaaa gagcgcgggg gaacccctct cctccctgac ggggggcgtt cacctccaca
     1741 ccctggaagt ggacggcgag gaggggtggg aacggctgct ggccgaactg ggtgaaaaga
     1801 actacttgcc ggagggacgc tgacggtgta tgctttacag gtgacaagac acctgtaaag
     1861 gaagtggtcc aatggcagga acggcgtctt cttctcctct tcgggggtac ctgatcaagg
     1921 tctcgaacgg gatggccctc gggctctttt cctctcttat catcggtctc atcctccgcc
     1981 agtgcgggca gctgctctcc ctcccccacc tggaacactt cggccgggtt gcccagcttc
     2041 tcatgggacc cgccatcggc gccggggttg cttttgccgt cggtgctcct cccctagggg
     2101 ttttttcctc tctcgtcgcc ggagccgtag gcgccggaac cttttccggc tctgccgccg
     2161 cggtgggcga gcccg
//
LOCUS       DAOTHD010000352         2175 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_59860_length_2175_cov_2.225472, whole genome shotgun sequence.
ACCESSION   DAOTHD010000352 DAOTHD010000000
VERSION     DAOTHD010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59860_length_2175_cov_2.225472"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="P5219_06665"
     CDS             <1..1322
                     /locus_tag="P5219_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26474.1"
                     /translation="EPADYITVEGYWADVTPEGHHSDKHIAANLFLYKDYLYVGTLNG
                     GFAIYESELPVSAAEEMTPAGFMEDLLKFPYASYLYRFDGDTWEDIVGPFGALSGYGP
                     GFDVPFNVYFWTAKEYDGRLYVGTFDWRSMFYGIDWNAAVGNPTLEQIMSVVFSHWEA
                     FFPGVEVPETPQEAMDIILNHGSPMGFDVFVSEDGVNFLPVTQDGFGNPGSHGARTMA
                     VAGSLPYLWLGTTNMYEGCSVWRHQWVNPPKDFTFSGDNYWVKLTTCPGAEDVSNFLV
                     ETDLSNPDPDWPGEVPDNVVFGRISWDGGPVCEAKIVIYFDECDEQAGIWWWDGDDWV
                     VLDGTWVLRTDDPDDDIWDGVDAGFCGALVIELPQPDQAGTGTIEPENANGVILGIGP
                     ASTEGFTNPDDQGEGENGGGGGGCSTGSVLPFGLLMLLPLLAVRGKR"
     gene            complement(1476..1904)
                     /gene="gspG"
                     /locus_tag="P5219_06670"
     CDS             complement(1476..1904)
                     /gene="gspG"
                     /locus_tag="P5219_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HRX26475.1"
                     /translation="MNRKRRSGFTLVEILVVVVILGLLATLVIPRVVGRGEEAKRTAA
                     LVQIREIEQALDLYKLDNGFYPTTEQGLEALVSRPGGLPEPRNYREDGYMKKVPSDPW
                     GRPYLYRSPGDHGEADLFSLGPDGAEGGEGKNRDITNWDE"
     gene            complement(1939..>2175)
                     /locus_tag="P5219_06675"
     CDS             complement(1939..>2175)
                     /locus_tag="P5219_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRX26476.1"
                     /translation="EGSFAEDAVYIVQVGEAGGELSEALQQVADNSWEIAENRMARLA
                     DLLEPAMVLVLGAIVGFVVLAILLPIFDLSTLIR"
BASE COUNT          395 a          661 c          729 g          390 t
ORIGIN      
        1 tcgaacccgc ggattacatc acggtcgagg ggtactgggc cgatgtcacg ccggagggac
       61 accacagcga taagcacatc gcggccaacc tgttcctgta caaggattac ctgtatgtcg
      121 gcaccctgaa cggggggttc gcgatctacg agtccgagct gccggtctcg gcggcggagg
      181 agatgacccc cgccgggttc atggaagacc tgctgaagtt cccctacgcc tcctacctgt
      241 accggttcga cggcgacacc tgggaggaca tcgtgggtcc gttcggggcc ctgtccggtt
      301 acggtcccgg cttcgacgtc ccgttcaacg tctatttctg gacggctaag gaatacgacg
      361 ggcggctgta cgtggggacg ttcgactggc ggtccatgtt ctacgggatc gactggaacg
      421 ctgccgtggg caaccccacg ctggaacaga tcatgagcgt cgtcttctcg cactgggagg
      481 cgttcttccc cggtgtcgag gtgccggaga ccccgcagga ggccatggac atcatcctga
      541 accatggttc gccgatgggc ttcgacgtct tcgtttccga ggacggcgtc aacttcctgc
      601 ccgtgacgca ggacgggttc gggaaccccg gatcgcacgg ggcgcgcacg atggcggtcg
      661 ccggatccct gccgtacctc tggctgggaa cgacgaacat gtacgaaggg tgctcggtct
      721 ggaggcacca gtgggtgaat cctcccaagg acttcacgtt cagcggggac aattactggg
      781 tcaagctgac cacgtgcccc ggggccgaag acgtgagcaa cttcctggtg gagacggacc
      841 tgtccaaccc cgatcctgac tggcccggcg aggttcccga caacgtggtc ttcggccgga
      901 tcagctggga cggcggtccc gtttgcgagg cgaagatcgt catctacttc gacgagtgcg
      961 acgagcaggc gggcatctgg tggtgggacg gcgacgactg ggtggttctc gacgggacct
     1021 gggtcctgag gacggacgat cccgatgacg atatctggga cggtgtcgat gccgggttct
     1081 gcggagccct cgtcatcgag ctgcctcagc ccgaccaggc gggcacgggc accatcgagc
     1141 ccgagaacgc caacggtgtg atcctgggca tcggtcccgc gagcacggaa gggttcacga
     1201 accccgatga ccagggcgaa ggcgagaacg gaggcggagg cggaggctgc agcaccggat
     1261 cggtcctgcc cttcgggctg ctgatgctcc tgccgctcct ggccgttcgc gggaagcgct
     1321 aggcacaaat tcgaaaacgc accatgagag aaggagagct gtcaaccgaa aagccgcaag
     1381 agagcgagag cgaagaggag caccatgtca tgcggcaaaa ggaaggggaa ccggcgcagc
     1441 cggttcccct tccttattct tccaggggat ggtggctatt cgtcccagtt cgtgatgtcc
     1501 cggttcttgc cctcgccgcc ctcggcgccg tcgggcccaa gggagaagag gtccgcttcc
     1561 ccatggtcac cgggagagcg gtagaggtag gggcgtcccc acgggtccga agggaccttc
     1621 ttcatgtagc cgtcttcgcg gtagttgcgg ggttccggca gtccgcccgg cctggagacg
     1681 agcgcctcga gtccctgttc ggtcgtgggg tagaagccgt tgtccagctt gtagaggtcg
     1741 agggcctgct cgatctcccg gatctggacg agcgcggccg ttctcttggc ttcctcgccc
     1801 cttccgacga ccctggggat cacgagcgtg gccaaaagcc cgaggatgac gacgacgaca
     1861 aggatttcca cgagggtgaa gccgcttcgg cgttttcggt tcatcgggaa caacctcctt
     1921 gggctaattt tatcccgttc agcggataag cgtggacagg tcgaaaatgg gcaggaggat
     1981 cgccaggaca acgaagccca caatggctcc gagcacaagg accatggcgg gctccagcag
     2041 gtcggcgagg cgggccatcc ggttttccgc gatctcccag ctgttgtccg cgacctgctg
     2101 cagggcctca gacagctcgc cgcccgcctc accgacctgc acgatgtaga cggcgtcttc
     2161 cgcgaaggat ccctc
//
LOCUS       DAOTHD010000353         2170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_60034_length_2170_cov_2.864775, whole genome shotgun sequence.
ACCESSION   DAOTHD010000353 DAOTHD010000000
VERSION     DAOTHD010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60034_length_2170_cov_2.864775"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1891
                     /gene="topA"
                     /locus_tag="P5219_06680"
     CDS             <1..1891
                     /gene="topA"
                     /locus_tag="P5219_06680"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRX26477.1"
                     /translation="NASRVLLASDPDREGEAIAWHLCELLDIDPASPCRVRFYEITPG
                     AVREAVKNPEEVNMSKVEAQQARRILDRLVGYTLSPLLWKKIRRGLSAGRVQSVALAL
                     ICAREREIREFVPRAYWLVTVAAAAGDGRKYTLRADSLDGKSLWKEGKSLLIDSEQVV
                     EAILGEVEKFPLTVTDFKVRESLRAAPAPFKTSTLQQEAARRSGLSPRRTMSIAQELF
                     EGVSIPGRGPTGLITYMRTDSLRIAPEAVEKCRAYIASSFEPSYLPEKENAYSAKGRS
                     QDAHEAIRPTDVTITPESLEGILTPDQMKLYSLIWRRYVASQMTPARVANATLTASAG
                     RAGFRQLGETLLFSGWSAVWPLDLKGESLQPAAGGEVLSVEGTDREQKFTRPPARYSE
                     ATLIKVLEDEGVGRPSTYASIVETLYDRGYVVRNEERRLQSTPLGETVDEFLMKYFNG
                     ESLSSIVDTGFTARMEESLDDVEEGKVPWLAVLRDFWKNFTVTMAEAETAPAAQLPPP
                     EPIGEDCPECGKPLVLKNGRFGEFIGCSGYPECRFTRPVLVKTGAVCPKCGTGDVVKR
                     KSRKGKPFYGCSRYPECDFVSWNPPSGRECPECGAAMMTTGRKGGEECPKCGYVPPKE
                     TRDDD"
     gene            1884..>2170
                     /locus_tag="P5219_06685"
     CDS             1884..>2170
                     /locus_tag="P5219_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRX26478.1"
                     /translation="MTDRSVSVVGGGLSGAEAAWMLATRGIPVILYEMRPRATTPAHR
                     TDGLAEVVCSNSFGADSTSSPGGILKEELRALGSLILKCADEARVPAGKALA"
BASE COUNT          427 a          707 c          690 g          346 t
ORIGIN      
        1 caatgcctcc agggtccttc tcgcctcgga ccccgaccgg gaaggggagg ccatcgcgtg
       61 gcatctctgc gagctcctcg acatagaccc ggcgtctccc tgcagggtcc gattctacga
      121 gatcactccc ggagccgtcc gtgaagcggt caaaaacccc gaagaagtca acatgagcaa
      181 ggtggaggcc cagcaggcca ggcgcattct cgacaggctc gtgggataca ctctcagccc
      241 gctgctgtgg aaaaaaatca ggagaggcct ctccgcaggg agggtccagt ccgtggccct
      301 ggcccttatc tgcgccaggg aacgggagat ccgggagttt gtccccaggg cctactggct
      361 cgtcaccgtt gctgcagccg cgggggacgg caggaagtac accctccggg cggacagcct
      421 ggacgggaaa tccctctgga aagagggcaa atcccttctc attgattccg aacaggttgt
      481 ggaggccatt ctcggcgagg tggagaaatt ccccctcacc gtcaccgatt tcaaggtgcg
      541 cgagagcctg agggccgctc cggcgccgtt caagacaagc accctccagc aggaggccgc
      601 aagaaggtcc ggcctgtcgc cccgcagaac catgagcatt gcccaggagc tgttcgaggg
      661 agtctccatc cccggacggg gacccaccgg actgataaca tacatgcgca ccgacagcct
      721 caggatcgcc cccgaggccg ttgagaaatg cagggcctac atcgcctcct ccttcgagcc
      781 ctcctacctg cccgaaaagg agaacgccta ttcggccaag gggcggtccc aggacgccca
      841 cgaagccatc cggccgacgg atgtgaccat cacgccggaa agccttgagg ggatcctcac
      901 tcccgaccag atgaaactct attctctcat ctggcggcga tacgtggcat cccagatgac
      961 tcccgcccgg gtggccaacg ccaccctcac cgcttccgca ggcagggccg gcttcagaca
     1021 gctcggggag accctgctct tttccgggtg gagcgccgtg tggcccctgg acctcaaggg
     1081 ggagtccctc cagccggcgg ccggcggaga agtgctctcc gtggagggga cggacaggga
     1141 gcagaaattt acccgtcccc cggcacggta ttccgaggca accctcatca aggtcctcga
     1201 ggacgaggga gtcggacggc cgtccaccta cgcctccatc gtggaaaccc tctacgacag
     1261 ggggtacgtg gtccggaacg aggagcggcg gctccagtcc actcctctcg gtgagacggt
     1321 ggacgaattc ctgatgaagt acttcaacgg cgaaagcctg tcctccatag tggacaccgg
     1381 ctttaccgcc cgcatggagg agagcctgga cgacgtggaa gaggggaagg tcccctggct
     1441 tgccgtcctc cgggatttct ggaaaaattt taccgtcacc atggccgagg ccgagactgc
     1501 cccggcggcc cagcttcctc ctcccgaacc catcggcgag gactgccccg agtgcggcaa
     1561 accccttgtg ctgaaaaacg gacgcttcgg cgagttcatc ggctgcagcg gctaccccga
     1621 atgccggttt acccgtcccg tgctcgtcaa gacgggcgcc gtctgcccga aatgcggcac
     1681 cggcgacgtg gtgaagcgga aaagcagaaa aggcaagccg ttctacggct gttcccgcta
     1741 tcccgagtgc gatttcgttt cgtggaaccc cccgtcggga agggagtgcc ccgaatgcgg
     1801 agcggccatg atgactacgg gaaggaaggg cggcgaggag tgcccgaaat gcgggtacgt
     1861 gccgccgaag gagacccggg acgatgactg accggtccgt ttccgtagtc ggcggagggc
     1921 tttccggggc tgaagccgca tggatgctcg caacccgggg tatccccgtg atcctctatg
     1981 aaatgcggcc ccgagccaca acgccggccc acaggacgga cggactggcg gaggtggtct
     2041 gcagcaactc cttcggcgcc gattccacct cgagccctgg ggggatcctc aaggaagagc
     2101 tccgcgccct gggcagcctc atcctgaagt gcgccgatga ggcgcgggta ccggctggca
     2161 aggccctggc
//
LOCUS       DAOTHD010000354         2167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_60143_length_2167_cov_3.332860, whole genome shotgun sequence.
ACCESSION   DAOTHD010000354 DAOTHD010000000
VERSION     DAOTHD010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60143_length_2167_cov_3.332860"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="P5219_06690"
     CDS             complement(<1..356)
                     /locus_tag="P5219_06690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRX26479.1"
                     /translation="MIIYLGFEDMQAVLGLASQVEGLFGPMVGVADFETALEDCLKTN
                     RVLGCKEQNELCGAAIIDRKGNELCWFVVDSGKRGLGIGGRMLEKVIGELDSAREMTV
                     QTFAPGVPEGGAARRL"
     gene            complement(384..818)
                     /locus_tag="P5219_06695"
     CDS             complement(384..818)
                     /locus_tag="P5219_06695"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRX26480.1"
                     /translation="MSELHEEVSIRLINDKVRFTGVSSANPDSPVSIDYVPPLGDGMG
                     FRGLELFLLSFGGCSATAVVALLRKMGKTVSGFEVRAKGTRSEKPPIKLEKISVEFVL
                     ESKDAEEKDLRRAVQLAEESVCPVWQLIKNNVDVVPEYRVIK"
     gene            complement(932..1486)
                     /locus_tag="P5219_06700"
     CDS             complement(932..1486)
                     /locus_tag="P5219_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRX26481.1"
                     /translation="MKPVVIIVHEVRGITENLRLLASLLESEGYCVILPSLYGENYAG
                     SDGQESYDRFFRDVGIEKATEMIGEIITANDGAGITLLGFSVGATIAWLHSRNGKVRS
                     VVGFYGSRIRHFQEVEPLADVDLFFSIERGFNAEELVGRLNGKQNVAARLISGDHGFY
                     SKLDFDSPEIRETNRMILALLKGV"
     gene            complement(1530..>2167)
                     /locus_tag="P5219_06705"
     CDS             complement(1530..>2167)
                     /locus_tag="P5219_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CatA-like O-acetyltransferase"
                     /protein_id="HRX26482.1"
                     /translation="MKEIDIEAWSRKEHFQFFLRSDLPFYNVNFNVDITGLKEYARSC
                     GLSLTNTLLFLSVKALNRVENFRYRLMDGKVVLYDRINPSFACIRGNEELFRMITLEF
                     EDDLACFDARAKEAIELSTSYFDLSMLKGRSDFAFFSPLPWIPFTAVDHTTSLKKEDC
                     IPRISWGKFAQADGKVLLPFNIQVNHIFIDGVHVGQYYEKLHELIREQMKK"
BASE COUNT          438 a          684 c          542 g          503 t
ORIGIN      
        1 tacagccttc ttgccgctcc tccttcagga acgccgggcg caaaggtctg cacggtcatc
       61 tccctggcgg aatccagctc gccgatcact ttttccagca tccggccgcc gatccccagc
      121 cctcttttcc cggaatcaac gacgaaccag cacagctcgt tccctttccg gtcgatgata
      181 gccgcgccgc agagttcgtt ttgctccttg cagcccaata cccggttcgt tttcaggcag
      241 tcctcaaggg ccgtttcaaa atcggcgacc ccgaccatcg ggccgaaaag gccttccacc
      301 tgggacgcca gcccaagaac ggcctgcatg tcctcaaaac ccagatatat gatcattccc
      361 tgctccggcc gtctcttttc cggctatttt atgaccctgt attccggaac gacgtcgacg
      421 ttgttcttta tcagctgcca gacggggcag accgactcct ccgcaagctg gaccgccctc
      481 cggagatctt tttcctccgc gtccttcgac tcaaggacaa actcgacgga gatcttttca
      541 agttttatgg gaggcttctc gcttctggtg ccctttgccc tgacttcaaa tccggagacc
      601 gtcttcccca ttttccgcag gagagccaca accgctgtgg cgctgcatcc cccgaagctg
      661 agaagaaaca gttcaagccc cctgaatccc attccatccc ccaggggcgg gacataatcg
      721 atactcaccg gggagtccgg attggccgag gaaacgccgg taaaccggac cttgtcgttg
      781 atcagccgga ttgaaacttc ttcatgcagt tcggacatcg tatcgcccct tttttgcccg
      841 gatcgctccg gattgtgtgc cgctcctcct gtttctttcc ctgaatcccg cccggcacgc
      901 tgttcagtcc tcgtttgttc cctgagaccg tttatacccc tttcaacaac gccagaatca
      961 tccggttggt ctcccggatt tccggggagt cgaagtccag cttggaataa aagccgtggt
     1021 cgccggaaat cagccgggcc gcgacgttct gcttcccgtt cagcctgccc acgagttctt
     1081 cagcattgaa tccccgctcg atgctgaaga acagatccac atccgccagg ggctccacct
     1141 cctggaagtg cctgattctg gagccgtaaa accccaccac ggagcggacc ttcccgtttc
     1201 gcgagtgcag ccacgcgatc gtggcaccga cgctgaaccc gagcagggtg atcccggcgc
     1261 cgtcgttcgc ggttattatt tcgccgatca tctctgttgc cttctcaatc ccgacatccc
     1321 ggaaaaacct gtcgtaggat tcctgcccgt cgctcccggc gtagttttca ccgtagaggg
     1381 acggcaggat gacgcaatag ccttccgact caaggagcga ggccagcagc ctgagattct
     1441 ccgttatgcc ccggacctcg tgaacgatga tgacaaccgg cttcatctct ttttccccaa
     1501 cctgcagcac gctggaaccg aaccgggggt catttcttca tctgctcccg gatcagttca
     1561 tggagcttct cgtaatactg ccccacgtgc accccgtcga tgaaaatatg attcacctgg
     1621 atgttgaagg gaagcagcac cttcccgtcg gcttgcgcga attttcccca ggagatcctc
     1681 gggatgcagt cctctttctt caggctcgtc gtatggtcga cggcggtaaa gggaatccat
     1741 ggaaggggcg aaaagaaggc aaagtcggac cgccccttca gcatggacag atcgaaatag
     1801 gatgtgctga gctctattgc ctcctttgcc ctggcgtcga agcaggcgag atcatcctcg
     1861 aactccaggg taatcatccg gaacagctct tcatttcccc ggatgcaggc aaaggaaggg
     1921 ttgatccggt catacaggac cacttttccg tccatgaggc ggtaccggaa gttttccacc
     1981 cggttgagcg ccttcacgga aaggaaaagc agggtattgg tgagcgacag gccgcaggac
     2041 cgggcatatt ccttcagccc ggtgatgtca acgttgaagt tcacattgta gaacgggaga
     2101 tcgcttctca ggaaaaactg aaaatgctct ttcctgctcc atgcttcaat gtcgatttcc
     2161 ttcattt
//
LOCUS       DAOTHD010000355         2164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_60257_length_2164_cov_4.623992, whole genome shotgun sequence.
ACCESSION   DAOTHD010000355 DAOTHD010000000
VERSION     DAOTHD010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60257_length_2164_cov_4.623992"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1562
                     /locus_tag="P5219_06710"
     CDS             3..1562
                     /locus_tag="P5219_06710"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRX26483.1"
                     /translation="MSHRTIEELCEELLKKHEEAQGGGGAKAVGKQKEKGKGTARERL
                     EKLLDPGTFVELDEYVRHRCTNFGLEKTKYYTDGVVTGWGLVDGRKVYVYSQDFTVLG
                     GSLGEMHAAKICKVMDLALSTGCPVIGINDSGGARIQEAVDSLNGYGAIFYRNTISSG
                     VVPQISIIAGPTAGGAVYSPALTDYIFMVEGTGIMHITGPAVIKAVTGEEVTSEELGG
                     SMAHNAKSGNAHFTAKTEDECFSQVRKLLSYLPLNNLESAPEVPVADDPYRRAMELRS
                     IVPTNPNKGYDMRDVIRKVVDGEDFFEVQALWAKNILTGYGRVGGKVIGIIANQANNM
                     AGCLDINASDKASRFIRHCDAFNIPIVTFVDVPGYLPGTEQEFGGIIRHGAKMLYAYS
                     EATVPLITVVVRKAYGGAYLGMCSKALGADMVLAWPQAEIAVMGAEGAANIVFRKDIE
                     AAEDSTAMRSKKIEDYRDAFANPYVAAERGLVDRVILPETTREEIWQALVGNETKRSL
                     RPRRKHGVLPH"
     gene            1584..1997
                     /locus_tag="P5219_06715"
     CDS             1584..1997
                     /locus_tag="P5219_06715"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HRX26484.1"
                     /translation="MNPIHEQIEGVGGALTMSIIAFSVVFLVLGGLSGIIYGIKYMAT
                     ALERKKGGGSGTAPAPVAPAAAVQTVPAAAGGSDKGRLLAVISAAVAAVEGPGARITS
                     VRPSGGAAALAPRGGLEWKRVGIIETLGGLSRDWK"
BASE COUNT          533 a          651 c          686 g          294 t
ORIGIN      
        1 acatgtcaca ccggacaatt gaggagttgt gcgaggagct tctgaagaag cacgaggaag
       61 cgcagggagg cggcggtgcg aaggcggtcg ggaagcagaa ggagaaggga aagggaacgg
      121 cgcgggagcg tcttgagaag ctgctggacc ctggaacctt tgtggagctg gacgaatacg
      181 tccgacaccg gtgcaccaat ttcggacttg agaagacgaa gtactacacc gacggagtgg
      241 tcacgggatg gggacttgtg gacgggcgga aggtatacgt atacagccag gacttcaccg
      301 ttctcggagg gtcactggga gaaatgcacg cggcgaagat ctgcaaggtc atggaccttg
      361 cgctgtccac cggttgtccc gtcatcggga tcaacgacag cggtggagcg cggatccagg
      421 aagcggtgga ctcgctcaac ggatacggtg ccatcttcta ccggaacacc atcagcagcg
      481 gagtcgtccc gcagatcagc atcatagcgg gtcccacggc gggaggggcg gtctacagcc
      541 cggcgctcac ggactacatc ttcatggttg agggaacggg gatcatgcac atcaccggac
      601 cggcggtcat caaggccgtc acgggggaag aagtcaccag tgaagaactg ggaggctcca
      661 tggcgcacaa cgccaaaagc ggcaacgccc acttcacggc gaagacggaa gacgaatgct
      721 tcagccaggt caggaagctg ctgagctacc tccccctgaa caaccttgaa agcgcgccgg
      781 aagtaccggt ggcggacgac ccgtaccgga gggccatgga gctccggagc atcgtgccca
      841 ccaaccccaa caaagggtac gacatgcgtg acgtcatccg gaaagtcgtg gacggagaag
      901 acttctttga agtccaggcg ctgtgggcca aaaacatcct cacgggctac ggccgggtgg
      961 gagggaaagt catcggcatc atagccaacc aggccaacaa catggccgga tgccttgaca
     1021 tcaacgcgtc ggacaaagcc agccggttca tccggcactg cgacgccttc aacatcccca
     1081 tcgtcacctt cgtcgacgtt cccgggtacc tccccggcac cgaacaggaa ttcggaggca
     1141 tcatccggca cggcgccaaa atgctctatg cctacagcga agccaccgtg cccctcatca
     1201 ccgtggtcgt caggaaagcc tacggagggg catacctggg catgtgcagc aaggcattgg
     1261 gagcggacat ggttctcgcg tggccccagg ccgaaatcgc cgtcatggga gccgaaggag
     1321 cggccaacat cgtcttccgg aaagacatcg aagcggcgga agacagcacg gccatgagga
     1381 gcaaaaaaat cgaagactac cgtgacgcct tcgccaaccc ctacgtagcg gcggagcgag
     1441 gactggtcga ccgtgtcatc ctgcccgaaa ccacccggga agaaatctgg caggccctgg
     1501 tagggaacga aaccaagcgc agcctccgac cccggaggaa gcacggcgta ctgccccact
     1561 gacgggtgga aggaggaaac cccatgaacc ccatccatga acaaatcgaa ggagtgggcg
     1621 gcgccctcac catgtccatc atcgccttca gcgtcgtctt cctcgtcctt ggaggcctga
     1681 gcggcatcat ctacggcatc aagtacatgg ccacggccct tgagcggaaa aagggcggag
     1741 gaagcggaac agcccccgcg cccgtggctc ctgccgcggc agtccagacc gtcccggcgg
     1801 cagcgggagg aagtgacaaa gggaggcttc tcgccgtcat cagcgccgcg gtagccgccg
     1861 tcgaaggccc cggagcccgc atcacctcgg tgcgtccgag cgggggagcc gccgccctcg
     1921 ccccccgtgg cggccttgaa tggaaaaggg taggcatcat cgaaaccctg ggcggactca
     1981 gccgggactg gaaataaccc cggaagcggg gcaaaaaagc gccggcacgg aacgaccgga
     2041 aaagaagagg cagcacaacc acaaccccaa ggaggcgtag caccaccatg accaaaaaat
     2101 acaaaatcac cgtagacgga accgcctaca cagtagaagt cgaagacctc ggcgccggag
     2161 cccc
//
LOCUS       DAOTHD010000356         2159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_60438_length_2159_cov_3.270913, whole genome shotgun sequence.
ACCESSION   DAOTHD010000356 DAOTHD010000000
VERSION     DAOTHD010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60438_length_2159_cov_3.270913"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="P5219_06720"
     CDS             complement(<1..595)
                     /locus_tag="P5219_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRX26485.1"
                     /translation="MELIRVLIADDHKLFRDGVKKLLELESDIAVVGEAGDGEEALSL
                     IREKEPDILLFDINMPRVDGIQLVRELNNIPHKTRYVAISAYDDEDCLSALSAAGVLG
                     FVLKASGKVELLSAIRSAARGQAYVDPRVAGKLLTSFSRRKEENDQLYELTPREKEIL
                     YWLAQGLSNTEVADRMVLSEKTVKNHVSHVLKKLDLRD"
     gene            764..1582
                     /locus_tag="P5219_06725"
     CDS             764..1582
                     /locus_tag="P5219_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX26486.1"
                     /translation="MTRSAVPVLSLSLRKKIGLAAGRFRMIAPGDRIMIGLSGGKDSL
                     VLALALAGLRRRSPVPFSLAACFVDITGGETEVSSLREFCDSLDIPFFVRPHPIVGII
                     EVREERSPCSLCANIRRGILNTAAKEDGCNLLALGHNLDDVVETALMNLFHTGRFRAF
                     QPKFRQSRSGMTVIRPLVFSEERKIGKEARRLGLPVLPYVCPFSLETERVKAKSILDG
                     LSAGNPRIKYNILHALQCLDERDKWSEGGTDEVSPPSGDEVQGEMTWEKTADSL"
     gene            1555..>2159
                     /locus_tag="P5219_06730"
     CDS             1555..>2159
                     /locus_tag="P5219_06730"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRX26487.1"
                     /translation="MGKNGGQSLGPTPSEASRTFPLGPEFPGTDDTVTERYFQWDPLD
                     DPDFAHLIIGKGSIGGKGRSLLFAMAKLRDSTEETLKRVVFPPSLFIATDSFDYVLRQ
                     IPDLEELKKEEPEVIEYAFLQAKLPSEVSEEVLKFLEKITDPIVVRSSSVLEDSLKYS
                     FAGKYLSTFEFNDGETPLEERAAEVERDIKKIYARTFFPVAV"
BASE COUNT          450 a          644 c          611 g          454 t
ORIGIN      
        1 ggtcccggag gtcgagcttc ttgagaacgt gactcacatg gttcttcacc gtcttctccg
       61 aaagcaccat cctgtcggca acttccgtgt tgctcagtcc ctgggcgagc cagtagagga
      121 tttccttctc cctgggggtc agctcgtaaa gctgatcgtt ctcctctttt ctcctggaga
      181 aggaggtaag gagcttccca gcgacccggg gatccacgta ggcctgtccc cgtgcagccg
      241 agcggatggc ggagagcagt tccaccttcc ccgaggcctt gaggacgaaa ccgagcaccc
      301 ccgccgccga gagggcggaa agacagtctt cgtcgtcgta ggcgcttatg gctacatacc
      361 gtgttttgtg gggaatgttg ttcagctccc ggacaagctg tatgccgtcc acccggggca
      421 tgttgatgtc aaaaagaagg atgtccggtt ctttttccct gatgagggag agtgcttctt
      481 ctccgtcgcc cgcttccccg acgacggcga tatccgattc cagttccaga agttttttca
      541 ctccgtcgcg aaagagtttg tgatcatccg caatgagtac cctgatcaat tccatctccc
      601 cacccccgtc acgtgggccg aaaacggcct caactcctaa gaatgcgatt atgctatagt
      661 agacgttgcc cctgcttgta cggtaaagga atttccgcca tgggcatagg tcttaagaac
      721 tattctatca cagatttcct gattttcaga ggaggagcgc cctttgaccc gatccgccgt
      781 acccgtcctt tccctgtccc tcagaaaaaa aatcggcctc gcggcgggcc gcttccggat
      841 gatcgccccc ggagaccgga tcatgatcgg tctctcgggc ggaaaggaca gcctcgtatt
      901 ggcccttgcc ctcgcgggac tccgcagaag gagccccgtt cctttttccc tcgccgcctg
      961 cttcgtggac attaccggcg gggaaacgga ggtctcgtcc ctgcgggaat tctgcgattc
     1021 cctcgatatt ccctttttcg tccgccccca ccccatcgtc gggatcatcg aggtgcggga
     1081 agaacgatct ccctgcagcc tgtgcgccaa catccggcgg gggatactga acaccgcggc
     1141 gaaggaggat gggtgcaacc tcctcgccct gggacacaac ctggacgacg tggtggagac
     1201 ggcgctgatg aacctgttcc atacaggcag gttcagggct tttcagccga aattccggca
     1261 gagcagatcc ggcatgacgg tcatacggcc gctggtgttc tcggaagaga ggaaaatcgg
     1321 gaaggaagcc cgcaggctcg gcctgcccgt tctgccctac gtctgcccct tctccctcga
     1381 aacggaacgg gtaaaggcga aaagtatact tgacggactg agtgccggga atccgagaat
     1441 caagtacaat atattacatg cccttcagtg cctggacgaa cgggacaaat ggtccgaagg
     1501 agggacggac gaagtttccc cgccctccgg ggacgaggtt caaggagaga tgacatggga
     1561 aaaaacggcg gacagtcttt agggcccaca ccctccgaag cgtcgcggac gtttccctta
     1621 gggcccgagt ttcccggaac cgacgacacg gtgacggagc ggtatttcca atgggatcct
     1681 cttgacgatc ccgattttgc ccacctcatc atcgggaagg gcagcatcgg cggtaaggga
     1741 cggtccctgc tctttgccat ggccaaactc cgggacagca cggaggagac gctgaagcgg
     1801 gtggtctttc ccccgtctct tttcatcgcc accgattcct tcgattacgt gctccgccag
     1861 attcccgacc tggaggagct gaagaaggaa gagccggagg tcatcgagta cgccttcctc
     1921 caggcaaaac tcccctccga ggtgagcgaa gaggtcctga aattcctcga aaaaatcacc
     1981 gaccccatcg tggtgcggag cagcagcgtc ctcgaggact cgctgaaata ctcctttgcc
     2041 gggaaatacc tttccacttt cgagttcaac gacggagaaa cccctctgga agagcgggcg
     2101 gccgaggtgg agcgggacat caagaaaatt tacgccagga ccttctttcc cgtggcggt
//
LOCUS       DAOTHD010000357         2158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_60495_length_2158_cov_2.454113, whole genome shotgun sequence.
ACCESSION   DAOTHD010000357 DAOTHD010000000
VERSION     DAOTHD010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60495_length_2158_cov_2.454113"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..386)
                     /gene="purS"
                     /locus_tag="P5219_06735"
     CDS             complement(135..386)
                     /gene="purS"
                     /locus_tag="P5219_06735"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HRX26488.1"
                     /translation="MLRHVHVFVQLKDGVLDIQGKAVANSLVSLGHDSLGSAKVGKYI
                     QLWIEADSAAGARKEAEKMCDDLLVNPIIEQYSIEVEGE"
     gene            complement(386..1105)
                     /locus_tag="P5219_06740"
     CDS             complement(386..1105)
                     /locus_tag="P5219_06740"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRX26489.1"
                     /translation="MEKKEFLYEGKAKKVWTTDDPSVYVVEYKNSLTAFNALKKDTIE
                     GKGRLNNLISSVLFEYLGKKGVPTHFISRIDDLQQLVKKVSIIPIEVVVRNITTGTLC
                     KRLGVEEGKVLPRPLVEFYLKDDALGDPIITEDHALLFGWSTPEQLTKIKEITLKVNS
                     LLSEYFLALGIILVDFKLEFGTDMEGRLLLADEISPDTCRFWDRDTKNRLDKDRFRKD
                     LGDVLGAYEEIWRRISSAEAL"
     gene            1187..1828
                     /locus_tag="P5219_06745"
     CDS             1187..1828
                     /locus_tag="P5219_06745"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HRX26490.1"
                     /translation="MKALEGVPVTAIVLAGGYGRRMGGEKLFLEVNSTPLVLKVLRKV
                     SRFSGEILLSVAPRQTPYVSGMLSGIISQYGLRLVEDRHEGDGPLAGILEGLKQASLG
                     WSFVCGCDMPWISEPVVRTLWRRGEEDADVIVPRIGGYFEPLHAFYGRGCIPAIERAV
                     RSGERKITSFYDLVKVSAVDDEHFSHLPGYKKSFGNLNSRNDLLDPGNAHLFD"
     gene            1862..>2158
                     /locus_tag="P5219_06750"
     CDS             1862..>2158
                     /locus_tag="P5219_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRX26491.1"
                     /translation="MEQQYIRNFCIIAHIDHGKSTLADRLLESTSTIGLRDMKQQILD
                     SLDLERERGITIKLVPVKMNYTAKDGREYVLNLIDTPGHVDFGYEVSRSLAACEG"
BASE COUNT          477 a          566 c          604 g          511 t
ORIGIN      
        1 agaccggttc cttcagggag ctgctcttcc ctgtgccaga cggcgttcac ttcactgccg
       61 acggtgtccc gtactgcgtg aatgacgtcc tggtcacagt tgctccccgg aaaaatgaca
      121 acggcggtct tcacctactc tccctccact tcgatgctgt actgttcgat tatgggattg
      181 accaggagat cgtcgcacat tttttccgct tctttcctgg cgccggctgc cgaatctgct
      241 tcgatccaga gctggatgta cttcccgact tttgcggaac ccagggaatc atgaccgagg
      301 gaaacaagag aattggccac cgctttcccc tggatgtcga gaacgccgtc cttgagctgc
      361 acgaatacat ggacgtggcg gagcatcaca gggcctccgc agaggatatc cgtctccaga
      421 tttcttcgta ggctccgaga acgtctccca ggtctttccg gaaacggtcc ttgtccaggc
      481 ggtttttggt gtccctgtcc cagaaacggc aggtatcggg ggatatttcg tcagcgagaa
      541 gaaggcggcc ttccatgtct gttccgaact cgagcttgaa gtccaccagg atgattccga
      601 gggcgaggaa gtattctgag agaagagaat tgaccttgag ggtaatttcc tttattttag
      661 ttaactgctc cggggtcgac catccgaaaa gaagtgcatg atcctctgtt atgatagggt
      721 ctcccagggc gtcgtccttc aggtagaact caaccagggg gcgggggagc accttgcctt
      781 cctcaactcc gaggcgtttg cagagggttc cggtggtgat gttcctcaca acgacttcga
      841 tggggatgat ggagaccttt tttacaagct gctggagatc gtcgatcctg ctgataaaat
      901 gggtgggaac gccctttttc cccaggtact cgaacaggac ggagctgatg aggttgttga
      961 gccttccctt gccttcgatg gtgtcttttt tcagggcgtt gaaggccgta agggaatttt
     1021 tatattccac gacgtacacg gaaggatcgt cagtggtcca gacttttttc gcttttcctt
     1081 catacagaaa ctcttttttt tccatggggg cacctccggc agagtagtgt gctcacatta
     1141 tttactagat cgtaaaaaat gtcaataaaa aggagagcct cggaaaatga aggccttgga
     1201 aggggttccc gtaacggcta tagtcctcgc aggcggttat ggccgccgga tggggggaga
     1261 aaaactgttc ctcgaggtca actcgacgcc cctcgtcctg aaggtgctca ggaaagtatc
     1321 ccgtttttcg ggagaaatcc tcctttcagt ggcgccgaga caaacaccgt atgtttccgg
     1381 catgctctcg ggtataatct ctcagtacgg cctgcgcctc gtggaagaca ggcatgaagg
     1441 ggacggcccc cttgccggca tcctggaggg attgaagcag gcttccctgg ggtggtcctt
     1501 cgtctgcggg tgtgatatgc catggatctc ggagcccgtg gtcagaaccc tgtggcggcg
     1561 cggtgaagaa gacgccgacg tgatcgtgcc gagaatcggc ggatatttcg aacctctcca
     1621 cgctttttac ggcaggggat gcatacccgc catcgaaaga gcggttcgct cgggagaacg
     1681 gaagatcacg tcgttttatg acttggtgaa ggtgtccgca gttgatgatg agcatttctc
     1741 ccaccttccg ggatataaaa aatctttcgg caatctgaac agcagaaatg atctcctgga
     1801 tccgggaaac gctcacctct ttgattaacg gagattcagg cacttcggga aggagaacac
     1861 catggaacag cagtacatac gcaacttctg catcatcgcc cacatagacc acggcaagtc
     1921 caccctcgcc gaccgccttc ttgagagtac aagcaccatc ggcctgaggg atatgaagca
     1981 gcagatcctc gattctctcg acctggaacg ggaaaggggc ataaccatca agctcgttcc
     2041 cgtgaagatg aactacacgg cgaaggacgg cagagaatac gtgctcaacc tgatagacac
     2101 cccgggacac gtggacttcg gctacgaggt ttcccggtcc ctagcggcct gcgaggga
//
LOCUS       DAOTHD010000358         2155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_60584_length_2155_cov_3.391905, whole genome shotgun sequence.
ACCESSION   DAOTHD010000358 DAOTHD010000000
VERSION     DAOTHD010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60584_length_2155_cov_3.391905"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="P5219_06755"
     CDS             complement(<1..180)
                     /locus_tag="P5219_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26492.1"
                     /translation="MAKIECCWYIGESLLPWEASVAGTLARALIRSGVALHAYSGAGT
                     DLLNIPGLLSWRSMHT"
     gene            complement(207..1487)
                     /gene="tyrS"
                     /locus_tag="P5219_06760"
     CDS             complement(207..1487)
                     /gene="tyrS"
                     /locus_tag="P5219_06760"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRX26493.1"
                     /translation="MYSDALQVLRQRGHIEWCSNDDDLGALFRREMVTAYVGFDPTAD
                     SLHVGHLIPIMALAWIQRCGHRPIPLAGMGTGLIGDPSGKSKERNLLTIEKVKENLEG
                     VKKQLASFLDFHCGPNSALLINNYDWLGKLTFLEVLRDVGKHFSVNSMISREYVRSRL
                     EDPEKSISYTEFSYVLLQAYDFLHLFSNYGCRLQMGGNDQQGNIISGVDLIRKKTGGE
                     AFGATQPLLLTSSGTKFGKTEEGAVWLDPARTSPYKFYQFWMNVEDQSVEKLLKLFTF
                     LSLDEIAEIMTLHEKSPERREAQRRLAWEVTTIVHGAESAETVKKASSILFGESFEPR
                     ELSGEMLRTLSAEVPTGEALREFPLSLVDLLMSAGACPSKSEARRLIKGGGLYVNGER
                     VSDENRSLKEEDVLGDRYLFVRLGKKRFFMILFR"
     gene            complement(1544..>2155)
                     /locus_tag="P5219_06765"
     CDS             complement(1544..>2155)
                     /locus_tag="P5219_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26494.1"
                     /translation="EWSIPDTVGLGGKELDELFPGPLMVSLGGTGKLLVFSLPGILFQ
                     LPGRGAAGTSFAASFWNREWSSLVPAVEKLEGFPAGGTASIPFSIVCAANAEMLRFGV
                     IDGGTLKSEKQIADFVPLMGDAQRAVFWAYLDGPALAEAVRSLARTGKIIEKMGRSSG
                     VDMKKVLRTSEALEKAGILSVVMPSLEKGVFEWKISPSSVKDK"
BASE COUNT          452 a          621 c          566 g          516 t
ORIGIN      
        1 ggtgtgcatg gagcgccatg agagaagccc gggaatgttc aggagatccg ttccggctcc
       61 cgaataggca tgaagagcca ccccgcttct gatcagcgcc ctggcaaggg ttcccgcaac
      121 tgaggcttcc cagggcagga gggattctcc gatataccag cagcattcta ttttcgccat
      181 tttcattccc tccctccgcg gcttgtttat ctgaaaagaa tcatgaaaaa ccgttttttt
      241 ccgagccgga caaagagata ccggtcgccc aacacgtcct cttcttttaa ggaacggttc
      301 tcgtcggaca cccgttcacc gttcacgtac aggccgcctc ccttgatcag gcggcgtgct
      361 tcgctttttg aaggacaggc accggcggac atgagaagat ccaccagaga aaggggaaac
      421 tctctcaatg cctcccccgt ggggacttcc gccgaaaggg tccggagcat ttctcccgac
      481 agctcccggg gttcgaagga ttcaccgaag aggatcgagc tggctttctt caccgtttcc
      541 gcgctttccg ctccatgcac gatggtggtt acttcccatg cgaggcgtct ctgggcttcc
      601 cgtcgctccg gggatttttc gtgaagagtc atgatttccg caatttcgtc gagggacagg
      661 aatgtgaaga gcttcagcag cttttccacc gactgatcct ccacattcat ccagaactgg
      721 tagaacttgt acggcgaggt cctggcagga tcaagccaga cggctccttc ttcggtcttt
      781 ccgaattttg ttccggagga cgtcagaagg aggggctggg ttgccccgaa ggcttctcct
      841 cctgtttttt tccggatgag gtcaactccc gatatgatat tgccctgctg atcgtttcct
      901 cccatttgga gcctgcagcc gtaattcgag aaaagatgaa ggaaatcgta ggcctgaagc
      961 aggacgtacg agaattccgt gtaggaaatg ctcttctccg ggtcctcgag acggctcctg
     1021 acgtattccc tggaaatcat ggaattgacc gaaaagtgct tccccacgtc ccggaggacc
     1081 tccaggaaag taagttttcc gagccagtcg taattattga tcaggagagc ggagttcggg
     1141 ccgcagtgga aatcgagaaa agaggcaagc tgctttttca ctccctcgag attttctttc
     1201 actttttcaa tggtgagcag gtttctctcc ttgcttttcc cggaagggtc accgataagc
     1261 cccgttccca tgcctgcaag ggggatgggc cgatggccgc atctctgtat ccatgcgaga
     1321 gccatgatgg ggatcaggtg tccgacgtga aggctgtccg ccgtagggtc aaacccgacg
     1381 taggcggtga ccatctcccg ccggaagagg gcccctagat cgtcatcgtt gctgcaccat
     1441 tcgatgtgtc ccctttgcct gaggacctgt agcgcgtctg aatacatagt actccctcct
     1501 gatcgtttcc cccggcaggc tttcctcggc cgtcggggtt ccttcatttg tccttcacgg
     1561 atgaagggct tattttccat tcaaaaacgc ctttttccag tgaaggcatg acgactgaca
     1621 gtatccctgc tttttccagt gcttccgatg tccggagtac ctttttcatg tccacgccgg
     1681 agctccgtcc cattttttca attatctttc ctgtccttgc aaggctccga acagcttccg
     1741 caagagcagg cccgtcgaga taagcccaga aaacggcccg ttgtgcgtcc cccatgaggg
     1801 ggacaaaatc cgcaatctgc ttttcggact tcagagtgcc tccgtcaata acgccgaacc
     1861 tgagcatttc ggcattggcc gcgcacacga tggaaaaagg tatggatgca gtccctcccg
     1921 cgggaaatcc ttcaagtttt tccacagccg ggacgaggga actccactcc cggttccaga
     1981 aggaagctgc aaaggacgtt ccggcggctc ctcttcccgg aagctgaaag agaatccccg
     2041 gcagggagaa aacgagcagc ttgccggttc cgccgagaga caccatcagc ggtcccggaa
     2101 aaagctcatc cagttctttt cctccaaggc cgactgtatc cggaatgctc cattc
//
LOCUS       DAOTHD010000359         2153 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_60654_length_2153_cov_3.605815, whole genome shotgun sequence.
ACCESSION   DAOTHD010000359 DAOTHD010000000
VERSION     DAOTHD010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60654_length_2153_cov_3.605815"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="P5219_06770"
     CDS             <1..1059
                     /locus_tag="P5219_06770"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX26495.1"
                     /translation="PFLSQGTEFHLGPAVSDIVSTSLAGARDITVVERMRLAEVARQH
                     RLSMSGVVDTATAATAGKLSGARYFILGAVSRFGALLVVTARLVDVESGRVLGSFEQT
                     SRQGEDEIVLVSRNLSAELLAFFSGDAPAPGDPLKDYRYYLYEALGYYNLGEYRKSLP
                     FWEKMTQLSPRNGLLRFIAAGIYFQAGRHSDALIAAQQAVTWDPSFAEAHLLVGKAYF
                     LLGDNHKATPPLDRALELNPRLTEALFLKGSAFKNRKRTEEAADFFVEAIQTDETYVP
                     AYLALGQLLLENGAAEEAAGVLLSGMKHQPGNGNLRFLLGTAHLMRGNLQGAKEQAAA
                     LKSIDTALAKKLEQLCSQ"
     gene            1244..1828
                     /locus_tag="P5219_06775"
     CDS             1244..1828
                     /locus_tag="P5219_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26496.1"
                     /translation="MSVFTSKGMLEGRDLDEAVKLAGSVTKEGECKAAYLAGASLLRK
                     GEYDRGREFMLKALDGCYDLSVNFWGDMERLRTIAAGELALHAGTRGDYRTIIAVEEK
                     VAKDIATDRLLVRDAKCILQGRTKLGDILKFHKARAYQASKMTADAKNTLNELQFASG
                     KVFVDGEVVGLKDAISKLQLQVDGAAFLPFLKAV"
     gene            1851..>2153
                     /locus_tag="P5219_06780"
     CDS             1851..>2153
                     /locus_tag="P5219_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26497.1"
                     /translation="MGRVPAAVLLLPVFLGILLFPCPSAGEMVLAVPDFQAVGCMPYL
                     GSGVAEQVRAKLTGRGPWTLLESSQMAKIASEHRLSMSGLMDEKKAISVGKVLGAQY"
BASE COUNT          447 a          610 c          676 g          420 t
ORIGIN      
        1 cccttccttt cccagggaac ggaattccac ctcgggccgg ccgtctcgga catcgtctcc
       61 accagcctgg ccggggccag ggacatcacg gtggtcgaac ggatgcgcct cgcggaggtc
      121 gccagacagc accgcctttc catgtccgga gtggtggata cggcaaccgc tgccacagcg
      181 ggaaaactct ccggggccag atatttcatt ctcggcgcgg tcagccgttt cggcgccctt
      241 ctcgtggtca ccgcacggct ggtggacgtg gagtccggca gggttctcgg gagcttcgaa
      301 cagacttcac ggcagggaga ggatgagatc gtccttgtct cgagaaacct ctcggcggag
      361 cttctggcct ttttcagcgg agatgcccct gcgccgggcg atcctctgaa ggactacagg
      421 tactatctct acgaagccct gggctactac aacctgggtg agtacagaaa atcccttccc
      481 ttctgggaga agatgaccca gctcagtccc cgcaacggac tcctccggtt catcgccgcg
      541 gggatctact tccaggcggg gaggcactcc gacgccctta tcgccgccca gcaggcggtc
      601 acatgggatc cctccttcgc cgaagcccat cttctcgtcg ggaaagccta tttcctcctc
      661 ggggacaacc ataaggccac accgcccctt gacagggccc tggagctgaa tcccaggctg
      721 acggaagccc tttttctcaa gggatcggcc ttcaaaaaca ggaagcggac cgaagaggcg
      781 gccgatttct tcgtggaggc tatccagacc gatgaaacct atgttcccgc gtatctcgcc
      841 ctcggtcagc tgctcctgga gaacggggcg gcagaggaag ccgcaggcgt tcttctttcg
      901 ggaatgaagc accagccggg caacggcaat ctccggtttc ttctcggcac ggcccatctg
      961 atgcggggca atctccaggg agcgaaggaa caggccgcag ccctgaaatc catagatact
     1021 gcgctggcaa aaaaactgga acagctctgc agccagtgag agctgcaatc tgcagaaagg
     1081 gagtgacgcg atcatgaagt tgaaagccca tgtatcggca ggaatcctga ttctcggctt
     1141 cgctctcctc ctctccccag tccggggatt cggatgaggc ggaggaagcg acgggggcgg
     1201 cggggcgtcg tccacagcag aggagaaggc catcgagaga atagtgagcg tgtttacctc
     1261 gaagggaatg cttgagggca gggatctcga cgaggcggtc aagctggcgg ggtccgtcac
     1321 gaaggaaggg gaatgcaagg cggcatacct ggccggcgcc agcctgctgc gaaagggaga
     1381 gtatgacagg ggcagggagt tcatgctgaa ggcccttgac ggatgctacg atctgtccgt
     1441 gaatttctgg ggcgacatgg aacggctccg gaccatcgcg gccggagaac tcgccctcca
     1501 cgcgggcacc aggggagatt accggaccat catcgccgtt gaggagaaag tggcaaagga
     1561 cattgccacg gacaggcttc tggtgagaga cgcgaaatgc attctccagg gaaggaccaa
     1621 gctcggtgat atactgaaat tccacaaggc tcgggcgtac caggcttcga agatgaccgc
     1681 cgatgcaaag aacaccctga acgaactcca gttcgcttcg gggaaagtgt tcgttgacgg
     1741 cgaagtggtc ggtttgaagg acgctatttc gaaactgcag cttcaggtgg acggagcggc
     1801 ctttctgcct ttcctgaagg ctgtttgacg tgtttcggga ggaatgagtg atgggcagag
     1861 tgccggcggc tgttctgctg cttcctgttt ttctggggat ccttttgttc ccctgccctt
     1921 cagcgggaga aatggttctt gccgtacctg atttccaggc cgtgggctgc atgccctacc
     1981 ttggctccgg agtggctgaa caggtaaggg cgaaactgac gggccggggc ccgtggacac
     2041 ttcttgaatc ctcccagatg gcgaagatcg cctcggaaca tcgtctctcc atgtccgggc
     2101 tgatggatga aaagaaagcg atatctgtcg gaaaggttct cggggcccaa tat
//
LOCUS       DAOTHD010000360         2135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_61353_length_2135_cov_2.863462, whole genome shotgun sequence.
ACCESSION   DAOTHD010000360 DAOTHD010000000
VERSION     DAOTHD010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61353_length_2135_cov_2.863462"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="P5219_06785"
     CDS             complement(<1..248)
                     /locus_tag="P5219_06785"
                     /inference="COORDINATES: protein motif:HMM:NF013538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HRX26498.1"
                     /translation="MKLLTRGSTLALLQAERMAAPLRERGLAVEIVPLSTRGDRDTVS
                     PLCSFGGSGAFSGCIEEALLGGKGDGAVHSLKDVPSCCR"
     gene            complement(245..739)
                     /locus_tag="P5219_06790"
     CDS             complement(245..739)
                     /locus_tag="P5219_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRX26499.1"
                     /translation="MTEQRPGNFSLMICLSPASGPILAVGGGPVGLRKIRTLLDGGAA
                     VDLVSPEAVPELQTLAAEGTIRWERRRAEREDFLEHRLALLALPPEETADILPLAEGT
                     GCLLNCCGAPESGSWALAAQFRWEGFVVGAGSGGGDPAGSAALKQRLLRSLEGMTEEK
                     ENRP"
     gene            complement(732..1880)
                     /locus_tag="P5219_06795"
     CDS             complement(732..1880)
                     /locus_tag="P5219_06795"
                     /inference="COORDINATES: protein motif:HMM:NF017051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26500.1"
                     /translation="MKSLLCLSLDYTAVSTAERDEAKNSWSFLPALREKGRILEAVPV
                     STCNRTEIYLRLPPGGTVPEEILHPRVRTLSGEEAVLHLLRVLLGLESMACGESSIVG
                     QVRESYDGAGDLCGPWLHRLFQGCLRMAKLLRTCYHPGREPSVPRLMAEKLREHPSFP
                     SIPALVVGAGEIGEETARILAGLSVPVSVTNRSGERGRSLAETLGAPFLPWEDWRERA
                     ADFGALFFCTGAPAPLFSLAERREGQRIFDLGSPPQVERNAGGSPFLLAMDDLAVEAA
                     AITAEYRTQLRRLESEAAEAARSVCAELTSLSAETYKRLVLLRAERIVEERADLTARK
                     TGADRESLRRMGWSVVKALLSPAFAESDPHARRLWKILSGDMEEPADD"
     gene            complement(1877..>2135)
                     /locus_tag="P5219_06800"
     CDS             complement(1877..>2135)
                     /locus_tag="P5219_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26501.1"
                     /translation="DLEEKLAAVRQIFGAAGYGEVHLVRDAGRPGASVRRLPLEDLSA
                     DGVDMRTLLLLPGSSVERVGDLLLDRRGYRAERAGKRGADS"
BASE COUNT          330 a          731 c          760 g          314 t
ORIGIN      
        1 cgacagcagg agggaacgtc cttgaggctg tggacggccc cgtctccctt tccgccgagg
       61 agggcttcct cgatgcagcc ggagaaggcg ccgctgccac cgaaggagca gagggggctg
      121 acggtgtccc ggtcgccccg ggtggacagg ggaacgattt ccacggccag gcccctctcc
      181 cggagcgggg cggccatccg ttcagcctgg agcagtgcga gggtgctgcc cctggtaagc
      241 agtttcatgg gcggttctcc ttttcttcag tcatgccttc gaggcttcgc aggagccgtt
      301 gtttaagggc ggccgacccg gcggggtcgc cccctccgct tcccgcgccg accacaaagc
      361 cttcccaccg gaactgggcc gccagggccc aggaaccgga ttccggggcg ccgcagcagt
      421 tgagcaggca tcccgtaccc tccgccaggg gaaggatgtc tgccgtctcc tccggcggca
      481 gggccagcag ggcgaggcgg tgctcgagaa aatcctcccg ttccgccctc cggcgctccc
      541 accggatggt tccctccgcg gcgagggtct ggagttccgg cacggcctcc ggagagacga
      601 ggtccacggc ggctcccccg tccaggaggg tgcggatctt ccgcaggccc acgggacctc
      661 ctccgaccgc gaggatgggt cccgaagccg gggagaggca gatcatgagg gagaagttcc
      721 cgggccgctg ctcagtcatc ggccggttcc tccatgtcgc cggagagaat cttccagagc
      781 ctccgggcgt gggggtccga ttcggcgaag gcgggggaga ggagggcttt caccacgctc
      841 catcccatgc ggcggaggga ttcccggtcg gcgccggtct tccgggcggt gaggtccgcc
      901 cgttcctcaa cgatgcgctc cgcccggagc aggacgagcc gcttgtaggt ctccgccgag
      961 agggaggtga gttccgcgca gacggaccgg gccgcctcgg cggcctcgct ctccagccgc
     1021 cggagctgtg tgcggtattc agccgttatc gccgcagcct cgacggcgag atcgtccatg
     1081 gccaggagga acggggagcc tcctgcgttc cgctccacct gggggggcga gccgaggtcg
     1141 aaaatcctct gcccctcccg ccgttcggca agggaaaaga ggggggccgg ggctcccgtg
     1201 cagaagaaga gggcgccgaa gtccgcagca cgctcccgcc agtcctccca gggcagaaag
     1261 ggcgcgccca aagtttccgc cagagagcgg ccccgttcac cggaacggtt tgtcaccgac
     1321 acgggaacgg agagcccggc gaggatccgg gcggtttcct cccctatctc accggccccc
     1381 acgaccaggg ccggaatgga aggaaaggag gggtgctccc ggagcttttc cgccatgagc
     1441 cggggcacgg agggttcccg gccggggtgg tagcaggtcc gcaggagctt ggccatccgg
     1501 aggcagccct ggaaaagacg gtggagccag gggccgcaga ggtcgcccgc tccgtcgtag
     1561 ctctcccgga cctgccccac gatggaggac tcgccgcagg ccatgctctc caggccgagg
     1621 agcacccgga ggaggtggag caccgcctcc tcgccggaga gggtccggac ccgggggtgg
     1681 agtatctcct ccggcacggt gccgcccggg ggcagtcgga ggtagatttc cgtccggttg
     1741 caggtggaca cagggaccgc ctcgaggatc cgccctttct ccctgagggc cgggaggaag
     1801 gaccaggagt ttttcgcctc gtcccgctca gcggtggaga cagccgtgta gtccagggag
     1861 aggcagagga ggctcttcat gagtccgccc cccgttttcc ggcccgctcc gcccggtagc
     1921 ccctccggtc cagcaggagg tctcccacgc gctccaccga cgacccggga agaagcagga
     1981 gggtgcgcat gtccacgccg tccgccgaca ggtcttccag ggggagcctc cggaccgagg
     2041 caccgggccg tccggcgtcc ctgacgaggt ggacttcccc atagccggcg gcgccgaaga
     2101 tctgccgcac cgccgccagc ttttcctcca ggtcc
//
LOCUS       DAOTHD010000361         2131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_61527_length_2131_cov_2.570328, whole genome shotgun sequence.
ACCESSION   DAOTHD010000361 DAOTHD010000000
VERSION     DAOTHD010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61527_length_2131_cov_2.570328"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            758..1987
                     /locus_tag="P5219_06805"
     CDS             758..1987
                     /locus_tag="P5219_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRX26502.1"
                     /translation="MTVFAQRMKTMEKSASIIRGLFGAMNDPGIISFGGGAPAREALP
                     VDIVREITNEVITRDGRGVEALQYGSVMGLPDLREIVVSELLAPKGVKADAGRIIITS
                     GGLEGMNLLCQLFIDPGDVILVESPTFVHCVEIFEMFQARCVSVAMDENGMVPEDLEA
                     KIREYRPKMVYVIPTFQNPTGRTLSLERRKKVAELGSRYDTIILEDDPYRDIRYSGTD
                     LPPIKAFDTTGHTVLANSFSKIFSPGSRLGYVLASEEITAKLFDAKTATNSHTSMLPQ
                     VICAEFFKRGYYPAHHRMICDLYRERRDVMIESIDTYFPKGTVRTFPDGGLFTWARLP
                     GGINTTGLLAESLASPDVRVAYVAGEGFFIEGNGMGNDCMRISFGGVPPEKIRIGAER
                     LGKLICSRLDQGLSCEA"
BASE COUNT          449 a          673 c          618 g          391 t
ORIGIN      
        1 ggtcccggaa ggcccactgc tgctcctcca gggacagcag aaaccggtgc gccgtgctct
       61 tcggaatgcc ggttatctct tccacatcac ggatcgtcag gggcgccctg gacgaacaca
      121 ggcctccatg atcgccgtta ccttgacggc tccgtccatt tttcccccct gcccgttttc
      181 ctgtgccata catcaacctc cgaaaaatgg gacataatgt cccattttta tcatcaagag
      241 agtattctgt caataaggcg ctgacattcc atggcctgtc atcctgagga gcgcagcggc
      301 gatcgcgaag cacccagagc gaagcggagg gagcatcccc ggaggggaag aacctatctt
      361 tgcttgtcat cctgagaagc gcagcgacga aggatctcgg tgttgatcct tcgcccctca
      421 agaccgagat tcttcgggct tcgccctcag aatgacagca agggggagga ccccggggct
      481 gtctttgtgt ttgtcttcct gaggagcggg tttgggcgac aaaagatccg ctattgatcc
      541 caggattcaa gaatcgagat ccctcgcgtc cgctcgggat gacaacctaa ccctgtcgtc
      601 ccgaacgagc ggagcgagga gggacctcga ttctggttct cagcagattc acccccgcga
      661 tccctgcgct cgggatgacg ctgtatcctt ccccccgaaa tgatacaatg cgggcagtga
      721 acgggttgcc agtctgcccg agaaaagggg gagcatcatg acagtcttcg cccagagaat
      781 gaaaaccatg gaaaaatcgg cctctatcat ccggggcctc ttcggcgcca tgaacgatcc
      841 cggcattatc tccttcggag ggggagcccc ggccagggaa gccctgcccg tcgacatcgt
      901 ccgggagata acgaacgagg tgatcaccag ggacggcagg ggagtggagg ccctccagta
      961 cggcagcgtc atgggcctgc cggacctccg tgaaatcgtg gtgagcgaac tcctcgcccc
     1021 caagggcgtg aaggccgacg ccggcagaat tatcatcacc agcggcggcc tggagggtat
     1081 gaacctcctc tgccagctct tcatcgaccc cggcgacgtc attttggtgg agtcgcccac
     1141 cttcgtccac tgcgtggaga tcttcgagat gttccaggcc cggtgcgtca gcgtcgccat
     1201 ggacgagaac ggcatggtcc ccgaggatct ggaggccaag atcagggagt acaggccgaa
     1261 gatggtctac gtcatcccca ccttccagaa tcccaccggc cgcaccctct ccctcgaacg
     1321 gagaaagaaa gtggccgaac tcggcagccg gtacgacacc atcatcctcg aggacgaccc
     1381 ctaccgggac atccgctaca gcggcacgga cctgcccccc atcaaggcct tcgacaccac
     1441 ggggcacacc gtcctcgcca acagcttctc caagatcttc tcccccggca gcaggctggg
     1501 ctacgtcctc gcgtcggagg aaatcacggc aaagctcttc gacgccaaga cggccaccaa
     1561 ctcccacacg tccatgctgc cccaggtcat ctgcgccgag ttcttcaagc gggggtacta
     1621 cccggcccac cacaggatga tctgcgatct ctaccgggag cggcgggacg tcatgatcga
     1681 gtccatcgac acatacttcc cgaagggaac ggtgcggacc ttccccgacg gggggctctt
     1741 cacctgggcc cggcttcccg gaggcatcaa caccaccgga ctgctggcag aatcgctggc
     1801 ctcgcccgac gtgcgggtgg cctacgtggc gggggaaggc ttcttcatcg agggcaacgg
     1861 catgggaaac gactgcatgc ggatcagctt cggcggggtt cctccggaga aaatccgtat
     1921 cggggccgag cgcctgggca aactcatctg cagcaggctt gaccagggac tcagctgcga
     1981 ggcatagaag acatctcacc tgagcgtcat cccggggcag gactcatgag gctgtcatcc
     2041 tgagagtgac cgctgcccgg gatgacagcc tcatgagtat agtataatat gcaatgtata
     2101 tgatcttctg attcacggga ggacgccatg c
//
LOCUS       DAOTHD010000362         2128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_61634_length_2128_cov_4.227207, whole genome shotgun sequence.
ACCESSION   DAOTHD010000362 DAOTHD010000000
VERSION     DAOTHD010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61634_length_2128_cov_4.227207"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="P5219_06810"
     CDS             <1..707
                     /locus_tag="P5219_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26503.1"
                     /translation="ETLARFSAAIAERAGRERLAAPVYLDEDLLEIPSLAILLLSKEM
                     IRLANYIEMYCQVLFVSSPNGDRTFQELPGAIRELSEACEEYMYGIHIPSDDDVLREN
                     YSTVSYSMVSFRQMAKILSGELRLLAEAGVGGQLARELGKTTWDELTRVAMTDIRLAL
                     RAFALADVDFAAVAGKYEREFEELDRRIRLRLGADALNRRELAPLVDFLSQIFLLVKT
                     SLEIARGEAYREGTAR"
     gene            732..1463
                     /gene="phoU"
                     /locus_tag="P5219_06815"
     CDS             732..1463
                     /gene="phoU"
                     /locus_tag="P5219_06815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRX26504.1"
                     /translation="MTHGKNNKSIQAFLYASDRERIVTTVIRMGEMAADALHNSVNAL
                     FNHDAVLAQKVIFNDDFIDRLEEAVDQECLGSIAMRSPGKEELCFVFGVLKIITDLER
                     IGDQAVNIAEKSLKIKCATTFPKKDRILSMLDTVLSMFRHALEAFRTGDREAASALWK
                     QDDEVDEIYAQCYDEFLDSAITGAAGNHEEVERGAVELWAARHLERAGDHVINIAERV
                     YFMVEGKYFPSREEREKNRKNGVTD"
     gene            1589..>2128
                     /locus_tag="P5219_06820"
     CDS             1589..>2128
                     /locus_tag="P5219_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRX26505.1"
                     /translation="MKKAVGIDLGTTNSVIAVIEGGTPEVVANAEGGRTTPSVVAFTR
                     DGERLVGSLAKRQATLNPDGTLFSVKRFIGRKYSEVGSELKIVPYKVSEGPNGAVRFS
                     VNGKEYAPEEISASVLKKLVTDASAYLGEKIADVVITVPAYFNDAQRQATKDAGTIAG
                     LNVLRVVNEPTAAALAYGLE"
BASE COUNT          470 a          605 c          667 g          386 t
ORIGIN      
        1 cggaaaccct cgcccgattc tccgccgcca tcgccgagcg agcggggcgg gagcgtctgg
       61 ccgccccggt gtaccttgac gaggatctgc tggaaattcc gtccctggcc atcctgctgc
      121 tttcgaaaga gatgatcagg ctggcgaatt acatcgaaat gtactgccag gtgctcttcg
      181 tttcgtctcc gaacggggac aggaccttcc aggagctgcc cggggcaatc cgggagcttt
      241 cggaggcctg cgaggagtat atgtacggca ttcacatacc ctcggacgac gacgttcttc
      301 gcgaaaatta ctccacggtg tcctattcca tggtatcctt ccggcagatg gcgaaaattc
      361 tttccgggga gctgcggctt ctcgccgagg caggagtggg aggccagctc gccagggaac
      421 tcgggaagac cacctgggac gagctcactc gggtggccat gacggacatc cggctcgccc
      481 tgagggcctt cgcccttgcg gacgtggatt tcgccgccgt tgccggaaaa tatgaacggg
      541 agttcgagga actggacagg aggattcggc tccgcctcgg agcggacgcc cttaaccgca
      601 gggagctcgc ccctctcgtg gacttcctgt cccagatatt ccttctcgtg aagacatccc
      661 ttgaaattgc ccggggtgag gcctacaggg aaggtacggc gcggtaacag cccaggaggt
      721 ggagttgccc agtgacccac ggaaaaaaca acaagtccat tcaggccttt ctctatgcgt
      781 ccgaccggga gcgcatcgtc accacggtga tccggatggg ggagatggcg gcggatgccc
      841 ttcacaactc ggtgaatgcc cttttcaacc atgacgcggt cctggcccag aaagtcatct
      901 tcaacgacga cttcatcgac aggctggagg aggccgtgga ccaggagtgc ctcggatcga
      961 tcgccatgag gagccccgga aaggaagagc tctgcttcgt tttcggcgtc ctcaagataa
     1021 tcaccgacct ggagcggatc ggcgaccagg cggtgaacat cgctgaaaag tccctgaaga
     1081 tcaagtgcgc aaccactttc ccgaagaagg accggatcct gtccatgctg gacacggttc
     1141 tctccatgtt ccgccatgcc ctcgaggctt tccgcaccgg cgacagggag gccgcctccg
     1201 ccctctggaa gcaggacgac gaggtggacg agatctacgc ccagtgctac gatgaatttc
     1261 tcgattcggc catcaccggg gcagcgggaa accacgagga ggtcgagcgg ggggccgtgg
     1321 agctctgggc agcccgtcac ctcgaacggg cgggagatca cgtcatcaac attgcggagc
     1381 gggtctattt catggtggag ggcaaatatt tcccgagcag ggaagaacgg gaaaagaaca
     1441 ggaaaaacgg agtgactgac tgaaggaaac ctgcggggac gctctcgaaa aagagcgtcc
     1501 ccttttttct gaccaatctt gacagacgga aaaaaacggc tataatttcc atagtcagca
     1561 ttagtcagta aatcaggggg gagataccat gaaaaaagcg gtcggaatag atcttggaac
     1621 aacaaattca gtcatcgcag tcatagaggg cggtacgccg gaagtggtcg ccaacgccga
     1681 gggaggaagg acaacccctt cggtggtggc ctttacaagg gacggggaga ggctggtcgg
     1741 ctcccttgcc aagcgccagg caaccctcaa tcctgacggg acgctctttt cggtgaagcg
     1801 gttcatcggc aggaaataca gcgaagtggg gtcagagctg aagatcgtac cctacaaggt
     1861 ttccgagggg cccaacgggg ccgtccggtt ttccgtgaac gggaaggagt acgctccgga
     1921 ggagatcagc gcctcggtgc tgaaaaagct cgtgaccgac gcctctgcgt acctgggcga
     1981 gaagatcgcc gacgtggtca tcacggtgcc ggcctatttc aacgacgccc agaggcaggc
     2041 caccaaggat gccgggacca tcgccgggct gaacgtcctg cgtgtcgtca acgagcccac
     2101 ggcggcggcc ctcgcctatg gtctcgag
//
LOCUS       DAOTHD010000363         2118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_62003_length_2118_cov_3.904508, whole genome shotgun sequence.
ACCESSION   DAOTHD010000363 DAOTHD010000000
VERSION     DAOTHD010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62003_length_2118_cov_3.904508"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1561)
                     /locus_tag="P5219_06825"
     CDS             complement(89..1561)
                     /locus_tag="P5219_06825"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRX26506.1"
                     /translation="MTDRMLTSRKVPNPCGPEMPRLLARIFSTLLEAGEDNRDAAVEN
                     GLKSLGEFLRVERSYIFLFRREDGEKLMDNTHEWCAPRVHSEKARLQAVPVSLISRSM
                     DLFARGEVLNIPRVSDLSDESGTEKALLESQGIQSALMVPLTINGKLRGFLGFDAVHR
                     IREWSGDETALLSLLGKMFAGAFELWERTRLLREMNEDLEARIADRTEELGLILRTMD
                     EGYFVLDGGGDFLNVNEGFSRISGFSAGELLSMSVFDLLNGSFPGGLAEERSGNGQTS
                     GISFETTISRKDGSRRDISVTGRFLPETGTFAAFVRDISERVRRERTMNFRATHDPLT
                     NLPNRLCFLERVEQALKRAAETGDSLAVTMVDLNRFKRVNDALGHAEGDKVLGEIARI
                     LSGAVRDSDTAARWGGDEFVLLFEKVRDAADALALASRVSDAVRVSRHIQGRESVITA
                     SIGVALFPRHGHTADDLLRCADKAMYRIKNGKGGFLLYSG"
     gene            1867..>2118
                     /locus_tag="P5219_06830"
     CDS             1867..>2118
                     /locus_tag="P5219_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26507.1"
                     /translation="MFFTMLLVSALVILLIAPSAGHARTHTFFITKEQAYNGMLRADI
                     PPALVIDDGDTVVFNTVMLLGGNLSPEMSFDDMLALRTEM"
BASE COUNT          439 a          676 c          552 g          451 t
ORIGIN      
        1 agtctccatc agggatcacc ggtccgcttc gccgtccctc ctccttcggt ggaagtccgg
       61 tggggaccaa gaatctgcgc ccccgagttc acccggaata gagcagaaat cctccctttc
      121 cgttcttgat tctgtacatg gccttgtcgg cacaccggag caggtcatcg gccgtgtgcc
      181 catgccgggg gaagagagcc acaccgatgc tcgccgtaat gaccgactcc cgcccctgaa
      241 tgtgcctcga aacacgcacc gcgtccgaga ccctcgaggc cagggccagg gcatcggcgg
      301 catcccgcac cttctcgaac agaagaacga attcgtcacc gccccaccgg gcagccgtgt
      361 ccgagtccct cacggcaccg gagaggatcc gggcgatttc ccccagaacc ttgtcccctt
      421 cggcgtgccc gagggcgtcg ttcacccgct tgaaacggtt gaggtctacc atggttaccg
      481 ccagggaatc gccggtttcg gcagcccttt tgagtgcctg ttccaccctt tcgagaaagc
      541 aaagcctgtt cggcagattg gtcagcggat cgtgggtcgc cctgaaattc atggtccgct
      601 cccggcgtac ccgttcggaa atatccctga cgaatgcggc gaaggttccc gtctccggaa
      661 ggaaacggcc cgtcactgat atatccctcc gggaaccgtc ctttcggctg atcgtagtct
      721 caaaggagat ccccgaagtc tggccgttcc cggagcgctc ttccgccaat ccgccgggaa
      781 acgaaccgtt caggaggtca aagacggaca tggaaaggag ttccccggcg gaaaaaccgg
      841 agattcttga aaacccctcg ttcacgttga gaaaatcgcc tcccccgtcg agaacgaaat
      901 atccctcgtc catggtgcgc aggatcaggc cgagctcttc cgtgcggtcg gcaattctgg
      961 cctccaggtc ttcgttcatt tcccggagaa ggcgggtccg ttcccacagt tcgaaggctc
     1021 cggcgaacat cttcccgagc agggagagga gcgccgtttc atcccccgac cactcccgga
     1081 tcctgtgcac cgcgtcaaac ccgagaaagc cccggagttt tccgttgatc gtcagcggca
     1141 ccatcagggc ggactgtatt ccctgggact cgagaagcgc tttttccgtt ccggactcat
     1201 cggaaagatc cgagaccctg ggaatgttca gcacttcgcc cctggcgaaa agatccattg
     1261 accttgagat gagggacacg ggcacggcct ggagacgggc tttttccgaa tgcactcgtg
     1321 gagcacacca ttcatgggta ttgtccatga gcttttcgcc gtcctcccgc ctgaaaagga
     1381 aaatatagct cctctcaacc cggagaaatt cgccgaggct cttcagtccg ttttccacgg
     1441 cagcgtccct gttatcctcc cccgcttcga ggagggtgga gaaaatccgg gccaggagcc
     1501 ggggcatctc gggcccgcac ggattcggga ccttccgtga agtcagcatc ctgtccgtca
     1561 taagaaaacc cccatgggga aaggatttcc atctccgggc aggatctgaa gcacgacact
     1621 cgattggggg acagtcatgg tgcaacctcc aaaccgctat taaacatttt attttttcgc
     1681 ttttatttta tccatttata aaatttacaa ctcaatcgat atcaaaatta taagttcttc
     1741 ccctcctgca ggcaagggac ggatttcgtt ccgccggtgt cccgatcccg aacctcccgg
     1801 acatgctccc gcagaaaaat gctatactcg caccatacag aatccttttt tcggaggtgc
     1861 acaaccatgt ttttcacgat gctgcttgtt tccgcccttg tcattctcct gatcgccccg
     1921 tcggcgggac acgcacgaac ccacacgttc ttcatcacga aggaacaggc ctacaacggc
     1981 atgctccggg ccgatatacc ccccgccctc gtgatcgacg acggggacac cgtggttttc
     2041 aacacggtca tgctcctggg cgggaatctt tcccccgaaa tgtccttcga cgacatgctt
     2101 gcccttcgca cggaaatg
//
LOCUS       DAOTHD010000364         2118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_62016_length_2118_cov_3.276297, whole genome shotgun sequence.
ACCESSION   DAOTHD010000364 DAOTHD010000000
VERSION     DAOTHD010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62016_length_2118_cov_3.276297"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="P5219_06835"
     CDS             <1..1076
                     /locus_tag="P5219_06835"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRX26508.1"
                     /translation="SERSSVDFGFYGAAGNRPEHIAELAERGVAAFKTYLFRPHAGRE
                     GEFEGTCAEGDGNLFEIFEAVAKTGKLCAVHAENDDLIRSFTRDVRAGGGRDLAAHGR
                     ARPPLAEVSAVAKVLAFARATGVRLGICHVSTPEAMELIGKEKAEGRAVYAETCPQYL
                     FCDEESAAPLGPFAKFNPPIRKQADAEALWNYVRNGTVDYVGSDHGPFLLSEKEPGTE
                     DIFAAPAGSTGFEERLPLFVTAVRERKLSLRRMVDLLSANAARIFGLYPQKGTISVGS
                     DADLAAVNLHEPFFVRASRMKTAGKGIARLYEGRRLFGVVTMTMVRGGIVFSRDGTLP
                     QASGRGRDLFYRSSSDEGSFQGR"
     gene            1082..>2118
                     /locus_tag="P5219_06840"
     CDS             1082..>2118
                     /locus_tag="P5219_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRX26509.1"
                     /translation="MKADLVIRNGQIFVNGEFFEGGVAVKDGKTVAICEDRYLPEAET
                     VLDAEGNPVLPGVVDTHVHVRDPGHRERGTFVTETKAAAAGGVTCFLEHPISSPPPYS
                     PEILRNRVRVAAPQALVDYAFFGAAGAEFPDEVEKVAKEGIVAFKTFLHEAPEGRDEE
                     FRGLTMANDGEILDGFRAVAKTGLILTIHAENNDIIQRLIRQFRAEGKVGFEYHYKSR
                     PPITEIETVAKLLMFAKETGTRVSFAHISTPEAAELVKRAKLDGMDVYLETCPHYLFL
                     TEEMIDKYGPYAKGNPPLRSRESMEKLWDYVNDGSVDFIGSDHGPFLVSEKEKGMKDI
                     FTAAAGPACIE"
BASE COUNT          430 a          593 c          689 g          406 t
ORIGIN      
        1 cctcggagcg ttcttcggtg gatttcggct tttacggggc ggcgggaaac cgcccggaac
       61 atattgccga acttgcggag cggggcgtgg cggctttcaa gacgtatctc ttcaggcccc
      121 atgcgggaag ggaaggggaa ttcgagggaa cctgcgccga aggggacggc aatcttttcg
      181 aaatcttcga agcggtggcg aaaacaggga aattgtgtgc cgtccatgcc gaaaacgacg
      241 acctcatcag gagtttcacc cgggacgtga gagcaggggg aggcagggac ctggccgccc
      301 acgggagggc gaggcccccc ctggctgaag tttcggcggt ggcgaaggtt ctcgcgttcg
      361 cacgggcgac gggtgtccgg ctgggcatct gtcatgtctc caccccggag gccatggaac
      421 tcatagggaa ggaaaaggct gaaggaaggg ccgtatatgc ggaaacgtgt ccccagtacc
      481 tgttctgcga cgaggagtct gccgctcctc tcggcccctt cgccaagttc aatccgccca
      541 tccggaaaca agccgacgcc gaagcgctct ggaactatgt gagaaacgga accgtcgact
      601 atgtcggaag cgaccacggt cctttcctcc tctccgaaaa ggagccgggg acagaggata
      661 tttttgccgc tccggcgggg tccacggggt ttgaggaacg tctgcccctc ttcgtcacgg
      721 cagttcggga acgaaagctc tctctccgcc ggatggtgga tcttctttcc gccaacgccg
      781 ccaggatttt cggcctgtac cctcaaaagg gaaccatctc cgtgggatcc gacgccgacc
      841 tggcggcggt gaaccttcat gaaccgtttt tcgtgagggc gtcaaggatg aaaacggcgg
      901 gaaagggcat tgccaggctg tacgaggggc ggcgcctgtt cggcgtggtg acgatgacca
      961 tggtacgggg gggaattgtc ttttcaaggg acggaactct tcctcaggcc tccggccggg
     1021 gtcgggatct tttttatcgt tcttcttctg atgaaggttc atttcaaggg aggtaggtag
     1081 tgtgaaagcc gatctggtga tccgtaatgg tcagattttt gtcaacgggg agttttttga
     1141 aggcggagtg gcggtgaagg acggcaaaac cgttgcgatc tgcgaggaca ggtatctccc
     1201 ggaggcggag acggttctcg acgccgaagg caacccggtg ctgccgggag tggtggatac
     1261 ccacgtccat gtgcgggacc cggggcaccg ggagcggggc acttttgtca ccgaaacgaa
     1321 ggccgccgct gcgggagggg ttacctgctt ccttgagcac cctatttcct cgcctccccc
     1381 ctactctcct gaaatactca ggaacagggt ccgggtggcc gctccccagg ccctggtgga
     1441 ctatgccttt ttcggagccg ccggcgccga gtttcccgat gaagtggaga aggtggccaa
     1501 agaagggatc gtagccttca aaacgttcct tcacgaggca cctgaaggcc gggacgagga
     1561 attccggggg ctcaccatgg caaacgacgg agaaatcctg gacggcttcc gggctgttgc
     1621 gaagacaggg ctcatcctca cgatccacgc ggagaacaac gacatcatcc agcgtctgat
     1681 ccggcagttc cgtgccgagg ggaaggtggg cttcgaatac cactacaagt cccgccctcc
     1741 cattacggag atcgagactg tggccaagct cctgatgttc gcaaaggaga cgggcaccag
     1801 ggtcagcttc gcccacatct ccacccccga ggcggcggag ctggtgaagc gggcgaagct
     1861 ggacggcatg gacgtctacc tggagacctg cccccactat ctgttcctca cggaggaaat
     1921 gatcgacaag tacggtccct acgcgaaggg caacccgccc ctccgcagca gggagtccat
     1981 ggagaagctc tgggattatg tcaacgacgg atcggtggat ttcatcggca gcgaccatgg
     2041 tcccttcctc gtctcggaga aggagaaggg gatgaaggac attttcaccg ccgccgccgg
     2101 tcctgcatgc atcgagat
//
LOCUS       DAOTHD010000365         2118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_62034_length_2118_cov_2.157053, whole genome shotgun sequence.
ACCESSION   DAOTHD010000365 DAOTHD010000000
VERSION     DAOTHD010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62034_length_2118_cov_2.157053"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="P5219_06845"
     CDS             complement(<1..465)
                     /locus_tag="P5219_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26510.1"
                     /translation="MSFFEHFGLTNAADVATGMLMLVMICYAIGDIVSYKTKAVFSMI
                     FVTGFVFLFGFWYGFPKTIFQTTGTIAFAAVSLPIILVHMGTLMKLRDIKQEWKTVII
                     AFVGLVALSIGLFFIAGPILGREMALTAAGPISGGIVATIIVSEAAKKVGLEH"
     gene            complement(561..965)
                     /locus_tag="P5219_06850"
     CDS             complement(561..965)
                     /locus_tag="P5219_06850"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRX26511.1"
                     /translation="MGVMTVSTRVRPEDLAVVAETRGHGLDMDQSKQNGGREDTMKPM
                     EVVLAALGGCITMVALARAPKEGLDIRDLRLGMEGEYSAAGGFRTIRMTARLRSSSPE
                     DRLVEFFHAVERVCPVSNSLKARIELEVVREQ"
     gene            complement(1132..>2118)
                     /locus_tag="P5219_06855"
     CDS             complement(1132..>2118)
                     /locus_tag="P5219_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRX26512.1"
                     /translation="VFFSEGDLAETRSIFAEGMTALVPRGSGVLILLPGERPGSVGDL
                     LVRGLAPDMEGVLCSCPDADGTLDTIARLRPDCLVALPSQALRLARHPRSSRGRCLSA
                     VLLCSDYASDSLTSAVEDAWGCTVFRQYGLTETGYGGALSCGEGRGMHVMEDGFFFEI
                     VSPGTGELLPDGEEGEIVCTAFRAEGTPLLRYRTGDRGRFIPGDCSCGSFLRRIDVRG
                     RYGNGLALPGGWVPLADLDEALFSLPWLGGYEVRMNGSSPVFFLEVPSLPEGILSLPE
                     DLEREAGKKLRSLPGLDILPPEIGLCLAPPRDAPLAPSKRLVLRETHPDRTR"
BASE COUNT          441 a          655 c          645 g          377 t
ORIGIN      
        1 gtgctcgagc ccgaccttct tggcggcttc cgacacgatg atggtggcca cgatgccgcc
       61 ggagatggga ccggccgctg tgagggccat ttcacggccg aggattgggc cggcgatgaa
      121 gaaaaggccg atcgaaaggg ccacgagacc aacaaaggcg atgatgaccg tcttccattc
      181 ctgtttgatg tccctgagtt tcataagggt tcccatgtgg acaagaatga tgggaagact
      241 gaccgcggcg aaggcaatgg ttcccgtcgt ctggaagatg gtcttgggga agccgtacca
      301 gaacccgaaa aggaagacga agcccgtgac aaagatcatg gagaagacgg cctttgtctt
      361 gtaggacacg atatcgccga tggcgtagca gatcataacg agcatcagca tgcccgtagc
      421 aacgtcggcg gcattggtca gaccgaaatg ttcgaagaaa ctcattctat ccctcctgct
      481 cctcaaaaga aaagtcgtct cgaaaaatac actccgcttc tccggatgtt tccggagcct
      541 ttccgttcac tcccgctctc ttactgttcc cgaaccacct ccagctctat cctggccttc
      601 agcgaattgc tgaccggaca caccctctcg acagcgtgaa aaaactccac gagccggtct
      661 tccggtgagg aggaccggag ccgtgccgtc atccggatcg tccggaagcc gccggcggcg
      721 gaatattctc cctccatccc gagcctgagg tcgcggatat caagaccttc cttcggagcc
      781 cgcgccaggg ccaccatggt gatgcacccg ccaagggccg ccaggacgac ctccatgggt
      841 ttcatggtgt cctcccgccc gccgttctgc ttcgactggt ccatgtcgag cccgtgtccc
      901 cgggtctcgg ccaccacggc gagatcctct ggcctcaccc tggtggaaac ggtcatcact
      961 cccatgtgga gtctctcctc ctcttccggc aggcctggat tgcagaatcg aaaatgcaga
     1021 aacagagatg agcaattcta tcacagttca ccgttttgtg ggaatcttca gtttcaccat
     1081 gaaaaaaaca ggacggaagc ggacggaaac aggtacggtc ggcccggcgg cttacctggt
     1141 gcggtccgga tgggtttccc ggaggaccag ccgctttgac ggggccaatg gtgcgtcccg
     1201 cgggggggcg aggcaaagtc caatttccgg aggcaggatg tcgagaccgg ggagggaccg
     1261 gagttttttc ccggcctccc gctccaggtc ttccggaagg gacaggattc cctccggcag
     1321 ggagggaact tccaggaaaa aaacagggga gctgccgttc atcctgacct cgtacccgcc
     1381 gagccagggg agggaaaaca gggcttcgtc aagatcggcc aggggaaccc atccgccggg
     1441 aagggcaaga ccgttcccgt atcttcccct gacatcaatt ctgcggagaa aggaaccaca
     1501 ggagcagtct ccgggaataa acctcccccg gtccccggtg cggtaccgca gaaggggggt
     1561 tccctcggcg cggaaggccg tgcagactat ttccccttct tccccgtcgg ggaggagttc
     1621 tcccgtgccg ggggagacga tctcgaagaa gaagccgtcc tccataacgt gcattccccg
     1681 gccttcgccg caggagaggg cgccgccgta ccccgtctca gtaaggccgt attggcggaa
     1741 gactgtgcat ccccaggcat cctccactgc cgacgtgagg ctgtcggaag cataatcgga
     1801 gcagagcagg actgccgaca ggcaccttcc ccgggacgac cggggatgcc tcgccagccg
     1861 gagcgcctgg gaaggaaggg ccacgaggca gtctggcctg agccgggcga tggtgtcgag
     1921 ggtgccgtcc gcatccgggc aggagcacag cacgccctcc atgtccgggg caagcccccg
     1981 gacgagcagg tcccccacgc ttcccgggcg ctctccggga agaaggatga gcaccccgct
     2041 cccccggggc accagggctg tcatgccttc ggcgaagatg ctccgtgttt cggcaagatc
     2101 tccttccgag aagaatac
//
LOCUS       DAOTHD010000366         2115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_62157_length_2115_cov_3.434466, whole genome shotgun sequence.
ACCESSION   DAOTHD010000366 DAOTHD010000000
VERSION     DAOTHD010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62157_length_2115_cov_3.434466"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="P5219_06860"
     CDS             complement(<1..668)
                     /locus_tag="P5219_06860"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HRX26513.1"
                     /translation="MKKNNFLSDEIGSSLGSISGPFSVYIHIPFCLRKCPYCSFFSVP
                     ATPGGCEKYLDLLKKEILFYRAFLGKESEASTLYFGGGTPSLLTPEQWEGLLAFCGEN
                     IPISPSAEITVEANPESFTERHAAVWRNGRVSRVSIGVQSFIDGDLLWLERPHNASKA
                     CDAVLTAVREGFSVSADLMFGLRNQTLRSWASSVKIALELGVEHISLYQLTIVEGCVW
                     KSSPA"
     gene            complement(655..>2115)
                     /gene="lepA"
                     /locus_tag="P5219_06865"
     CDS             complement(655..>2115)
                     /gene="lepA"
                     /locus_tag="P5219_06865"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRX26514.1"
                     /translation="AYMAVEQGLEIIPVINKIDLPSSRPDYVIREIEDVVGLDCDHVI
                     LASAKEGKGIDDILERVVEKVPAPLGDREAPLQAMIFDSVYDNYKGVICYIRVVNGRI
                     RAGQTIRFMATGGVFPVEEVGVFRPGFQPCDELAAGEVGYVVSNIKTVAEAHTGDTIT
                     DNGRPAASPLPGYRKVKPVVFCGFYPVERDDYPQLRDALEKLTLNDSSINFEPETSVA
                     LGFGFRCGFLGLLHMDVSKERLRREFGVELVATAPNVVYEVVTKDGGLIEAHRPSDFP
                     EIGEIQEVREPVIKISLFVPTEYVGKVMKLCQDKRGTYISMDYLAPDRVRLMYELPLA
                     EFIIDFHDKLKSQTRGYASLDYEFTGLKAADLVRVDVLVSGEAADAFSFICHRDSAYH
                     RGQAAVRKLKELIPSQLFEVPIQASVGKKVIVRQNVKALRKDVLAKCYGGDITRKRKL
                     LEKQKEGKKRMKQIGKVSIPQEAFLAFLQVEEDEEK"
BASE COUNT          460 a          609 c          581 g          465 t
ORIGIN      
        1 gcaggggagg atttccatac gcacccttcg actatggtca gctggtagag ggatatgtgt
       61 tccactccca gttccagtgc aatcttcacc gaagaggccc aggaccggag cgtctggttc
      121 cgcagcccga acatcaggtc ggcgctgacg gaaaaacctt cccggaccgc cgtcaggacg
      181 gcatcacacg cctttgaggc gttatgaggg cgttcgagcc agagaagatc cccgtcaata
      241 aaactctgga caccgatgct caccctggaa acccgtccat tcctccagac ggcggcatgc
      301 ctttccgtaa aggattccgg attcgcctcc acggttattt ccgcagacgg tgaaatcgga
      361 atgttctcgc cgcagaatgc cagaaggccc tcccactgct caggggtgag cagtgacggt
      421 gtgccgcctc cgaaatacag ggtggacgct tcggattcct tcccgaggaa agcccggtag
      481 aaaaggatct cctttttcag gagatccagg tatttttcac acccgccggg tgttgcggga
      541 acgctgaaaa aggaacagta gggacatttc cggaggcaga agggaatatg gatgtaaacg
      601 gaaaagggtc cggaaatgct tcccagcgaa gaaccgattt cgtctgaaag gaagttattt
      661 ttcttcatcc tcttccacct ggaggaacgc aagaaacgcc tcctggggga tggaaacctt
      721 gccgatctgc ttcatccgtt tctttccttc cttttgcttt tcaagaagtt tccttttcct
      781 ggtgatgtcg cctccgtaac attttgcgag gacatctttt cggagggcct tcacattctg
      841 gcgcacgatg accttcttcc ctaccgaggc ctggatgggg acctcgaaga gctggctcgg
      901 gatgagctcc ttcagctttc ttactgctgc ttgtcccctg tgataggctg aatcccggtg
      961 gcagatgaaa gaaaaggcat ctgccgcctc gccgctcaca agcacatcga ccctcaccag
     1021 gtccgccgct ttcagaccgg tgaactcata atcaagggag gcataccctc tcgtctggga
     1081 tttgagcttg tcatgaaagt cgatgatgaa ttcagccagg gggagctcat acatcagcct
     1141 gaccctgtcc ggtgcgagat aatccatgga aatataggtg cccctcttgt cctggcagag
     1201 tttcattacc tttccgacgt actccgtggg aacaaaaaga gagatcttga tgaccggttc
     1261 ccggacttcc tggatctcgc cgatttcggg gaaatcggac ggcctgtggg cctcgatgag
     1321 gcccccgtcc ttcgtcacta cttcgtagac cacgttgggg gcagtggcca cgagttccac
     1381 accgaactcc cgccggagcc tttccttgga aacgtccatg tggagcagac cgagaaagcc
     1441 gcaccggaaa ccaaaaccga gggccacgga cgtctcgggc tcgaagttga tggatgagtc
     1501 attgagggtc aatttttcca gggcgtcccg gagttggggg taatcgtccc gctccacggg
     1561 ataaaacccg cagaacacca cgggtttgac cttgcggtag cccggcaggg gcgatgcggc
     1621 gggcctgccg ttgtccgtga tggtatcgcc tgtatgggct tccgccacag tttttatatt
     1681 ggacaccaca tagccgactt ccccggcggc cagttcatca cagggctgga agccggggcg
     1741 gaacacccct acctcctcca cggggaagac ccctccggtg gccatgaacc ggatggtctg
     1801 cccagcacgg atccttccgt tgaccacgcg gatatagcag atgaccccct tgtaattatc
     1861 gtagacggaa tcgaaaatca tcgcctggag aggagcttcc cggtcgccca aaggagcggg
     1921 aactttctcc actacccgtt ccaggatatc atcaatgccc ttcccctcct tggcgctggc
     1981 gaggatgaca tgatcgcagt caaggccaac aacgtcttcg atttcccgaa tcacgtagtc
     2041 cggcctggaa gagggaaggt caatcttgtt gatcaccggg atgatctcaa gcccctgttc
     2101 gactgccatg taggc
//
LOCUS       DAOTHD010000367         2113 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_62261_length_2113_cov_2.613217, whole genome shotgun sequence.
ACCESSION   DAOTHD010000367 DAOTHD010000000
VERSION     DAOTHD010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62261_length_2113_cov_2.613217"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..>2113
                     /locus_tag="P5219_06870"
     CDS             304..>2113
                     /locus_tag="P5219_06870"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HRX26515.1"
                     /translation="MKRCCSVGGFVRLPVLWIILAAAVVAAGALVSFWRMGEADLFMR
                     EELSRQTRLLTETIPPDQIRALSGTEDDLSRPEYLRFKKQFRSALQAFPGSKFLTLVG
                     RREDGTVFFYADSELPGSGDESPPGDVYEEMDPGFLPAFEEQRPVTVGPITNRWGTWV
                     STWHPIPDPKTGRVFAALALDISGDNWKKYVRGAAVLPFGTGLALAAVLLGGCLALRL
                     RSGLQGDERWRLRHIEAVCALTAGLILTFSVSRLVHERENLDRRYSFSVISDARSAEL
                     NNLFGSLRGVALESLGRYMESSGEVSGEEFSEFVRHIVSLPEVESAAWIPAGENPVSY
                     SVRYRYPSAPGNDSGSYEVNAEAMALAAATGLPAAGSIRSISDLNGIRHCLCVFRPVY
                     RQEEGLPRLRGFVALSLRLDTIVNKAQRGGEGDAAMRLWELGHDGPRLAAGPDSPDLP
                     GRFSGSSVSPGQPLNGVRPIFAFGKTFCAELYPGPLFRVHHPVRAGWMALAAGFFITI
                     AVTVLLGNAVHGREVLERVVAGRTAELRESEARFRSLVEDVPFPVSVISPEGRVLFTN
                     SRGEEFFGIQSARTTGKHLLTELHILVNPGAFPGFVD"
BASE COUNT          348 a          634 c          677 g          454 t
ORIGIN      
        1 cgggagaaca tcctcgaggg cggaacgggg aacatcgtct cggtggagcg gaaccgcccc
       61 ccgctgaagg agcggctgag agccttccgg agcaggcttt cccgggaggg agactgatcg
      121 cgggggattt tgagcatatt gttttcttcc cgtcctttgg aattagccgt aggtgtttta
      181 taatcaaatg attattattc tgcccggctg tggaattccc cgcaggggaa acggggctga
      241 aagcctgtcg ctctcaaaga atagggaggc tgaagatgtg aattcggaaa ggcaacgttc
      301 cggatgaaac ggtgttgttc cgttggcggc tttgtccggc tgcctgtact ctggataatc
      361 ctcgccgccg cggtggttgc cgctggggct cttgtgagct tctggagaat gggggaggct
      421 gacctcttta tgagggaaga actgagccgc cagacccgtc ttctgacgga gacgatccct
      481 ccggaccaga tcagggccct ttcagggact gaggacgatc tttcccgtcc cgagtacctg
      541 cgcttcaaaa agcaattccg ctccgccctc caggcttttc ccggaagcaa gttccttacc
      601 cttgtcggac gccgtgagga cgggaccgta ttcttttacg ccgactcgga actgcccggt
      661 tccggggacg agtctcctcc gggcgacgtg tacgaggaaa tggatccggg attcctgccg
      721 gcctttgaag aacagcgccc ggttaccgtg gggcccatca ccaaccggtg ggggacatgg
      781 gtctccacct ggcatcccat accagacccg aaaaccggca gggtttttgc cgccctggct
      841 ctggacatca gcggagacaa ctggaagaag tatgtccgcg gcgctgctgt cctgcccttc
      901 ggcacggggc ttgccctcgc ggcggtcctt ctcgggggct gccttgccct caggctccgg
      961 tccggcctcc agggggacga acggtggcgt ctcaggcaca ttgaagcggt ttgtgccctg
     1021 accgcagggc tgatactgac cttttccgtt tcccggttgg tccacgagag ggaaaacctg
     1081 gaccggaggt actccttctc cgtaatctcc gatgcccgtt ccgccgagct gaacaatctt
     1141 ttcggcagcc tccgcggcgt tgccctcgag agcctcgggc ggtacatgga gtcctccggg
     1201 gaggtttccg gggaggagtt cagcgagttc gtgcggcaca ttgtctccct ccccgaagtg
     1261 gaatcggcgg cctggatccc cgcgggggag aacccggtca gctattccgt caggtaccgc
     1321 tatccttcgg ctcccgggaa tgattcagga tcctacgagg tgaacgccga ggccatggcc
     1381 ctggctgcag ccacgggtct ccccgcggcg gggagcatcc gttccatttc agacctgaac
     1441 ggtatcaggc attgcctgtg cgttttccgc ccggtctacc ggcaggagga agggcttccc
     1501 cggctccggg gattcgtcgc cctttccctc aggctcgaca ccatcgtgaa caaggcccag
     1561 aggggagggg aaggggatgc cgccatgaga ctctgggaac tcggccatga cggcccccgc
     1621 cttgcggcgg gccctgattc accggatctt cctggtcgtt tttcgggatc ctccgtttcc
     1681 cccggacagc ctctcaacgg cgtccgcccc atcttcgcct tcgggaagac cttttgcgcc
     1741 gagctgtatc cggggccgct gttccgggtt catcatccag tgcgggctgg atggatggcc
     1801 ttagctgccg gttttttcat caccatcgcg gtgacggtgc tcctgggcaa tgccgttcac
     1861 gggagagagg tcctggaacg ggtggttgcc gggaggacgg cggagctacg ggagagcgag
     1921 gcccggttcc gttccctggt ggaggatgtc ccctttcccg tgtcggtgat ttcgcctgag
     1981 ggaagggtgc tcttcaccaa ctcccggggg gaggagtttt tcggcataca gtctgcccgg
     2041 acgacgggga agcatcttct gactgaactg cacatcctgg tcaaccccgg ggcctttccc
     2101 ggctttgtgg acc
//
LOCUS       DAOTHD010000368         2112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_62297_length_2112_cov_2.911522, whole genome shotgun sequence.
ACCESSION   DAOTHD010000368 DAOTHD010000000
VERSION     DAOTHD010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62297_length_2112_cov_2.911522"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1644)
                     /locus_tag="P5219_06875"
     CDS             complement(<1..1644)
                     /locus_tag="P5219_06875"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRX26516.1"
                     /translation="MDFLLLLIVGITGIAALAFAFISYVRIDMFRVENERVSELSNII
                     HRGAMAFLYREYKWLLPFLVIVGVLLWVQIGGPAGFSFIIGGLCSAAAGYAGMVVATK
                     ANGKTAFAATRGMNDALTMAFKGGSVMGMSVVGIGVLGILFCYALFEDPTLITSFGMG
                     ASSIALFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGDIAGM
                     GADLFESYVNSIIAAMAIGLAAFGSKGVVYPLVLGGVGILAAIAGTTFVKVKDGGNAA
                     AALRMGTFVTGGIMIAGAFLVTLAMFREFGLFLSVLSGVGVGVLIGFVTEIYTSGDYS
                     SVKHIAESSKTGPATVILSGMAVGMKSTVIPVVLICAATLVSYYFSGLYGIACAAVGM
                     LSITGMALSVDAYGPIADNAGGIAEMSHLPKEVRKITDKLDAIGNTTAAMGKGLAIGS
                     AALTALALFAAYAHSVKLAAIDLNDPKVMVGLFLGGMLPFLFSSMSIQAVGRAAEAMI
                     DEVRRQFKEIPGIMEGTGQPEYEKCVDISTAAALKEMIIP"
     gene            complement(1770..>2112)
                     /locus_tag="P5219_06880"
     CDS             complement(1770..>2112)
                     /locus_tag="P5219_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26517.1"
                     /translation="KGLPLPRIWVGDPCGVPPDVKGVFAFFDRTASGSDGKKVFFPGR
                     EKDLAAMIIGMYERTGGNSPATLYVQGKKIPLKTFVSGMIAGCLAGLVLPLKGVNSLK
                     GGAEIYLKRRK"
BASE COUNT          462 a          605 c          654 g          391 t
ORIGIN      
        1 ggggatgatc atttccttca gggccgcggc ggtggagatg tccacgcact tctcgtattc
       61 gggctgccct gtgccttcca tgattccggg aatttccttg aactggcggc gcacttcgtc
      121 gatcatggct tccgccgcac ggcccaccgc ctggattgac atggagctga agaggaaggg
      181 aagcatcccg ccgaggaaga ggcccaccat gaccttggga tcgttgaggt cgatggcggc
      241 gagctttacg gaatgggcgt atgctgcgaa cagggccagg gcggtgaggg cggccgaacc
      301 gatggcgagt cccttgccca tggcggcggt ggtgtttccg atggcgtcga gcttgtcagt
      361 gatcttgcgt acttccttgg gaagatggct catctcggcg atgccgccgg cgttgtcggc
      421 gatgggaccg taggcgtcaa cgctcagggc cattccggtg atggaaagca tgcccacggc
      481 ggcgcaggcg atgccgtaca ggccgctgaa atagtagctg acgagggtgg cggcgcagat
      541 gagaaccacc gggatcaccg tggatttcat gccgacggcc attccggaga gaatgaccgt
      601 ggccgggccg gtcttggagg attcggcgat gtgcttgaca gaagagtagt cgccggaggt
      661 gtagatttca gtaacgaaac cgatgagcac gcccacaccc acaccggaga gaacggacag
      721 gaagaggccg aattccctga acatggcgag ggtgacgagg aaagcgccgg caatcatgat
      781 gccgccggtg acgaaggttc ccatacggag ggcggcagcg gcgtttccgc cgtccttcac
      841 cttcacgaaa gtggttccgg cgatggcggc aagaatgccc acgccgccca gaacgagggg
      901 ataaacgaca cctttcgatc caaaggccgc aagtccgatg gccatggcgg cgatgatgga
      961 gttcacgtag gactcgaaca ggtcggcacc catgccggca atgtcgccca cgttgtcgcc
     1021 cacgttgtcg gcgatgactg cggggtttct ggggtcgtcc tcggggatgc cggcttcaac
     1081 cttccccacc aggtcggctc cgacgtcggc agccttcgtg tagatgccgc cgcccacgcg
     1141 ggcgaagagg gcgatggagc tggcccccat gccgaagctg gtgatgaggg tcggatcttc
     1201 aaagagagcg tagcagaaaa ggatgcccag gacaccgatg ccgactacgg acatgcccat
     1261 gacgctgccg cccttgaaag ccatggtgag ggcgtcgttc atgcccctgg tggcggcgaa
     1321 ggccgtcttg ccgttggcct tggtggcgac caccatgccc gcgtatccgg ccgctgcgct
     1381 gcagagtccc ccgatgatga aggaaaagcc ggccggtccg ccgatctgga cccagaggag
     1441 cacgccgacg atgacaagga agggaagaag ccatttatat tccctgtaca ggaaagccat
     1501 ggcccccctg tgaatgatgt tcgaaagctc cgaaaccctt tcgttttcaa ccctgaacat
     1561 atcgatgcgc acataggata tgaaagcgaa cgcaagtgcg gcgattccgg tgatgccgac
     1621 gatcaacaac agtaaaaagt ccatttccac tacctcctcg ttttgatggg accagcgtcc
     1681 acaactcccg aaaaaagaaa aagcccggtg cgagagcaat tatagggagt aaaaatacaa
     1741 aaaacaagcg ttgattgcca aaaaacggct tatttccggc gtttcaggta gatttccgct
     1801 ccacctttca gcgagttcac acctttcaaa ggaagcacaa gtcccgccag acagccggcg
     1861 atcattcccg acacgaaagt tttcagggga atcttctttc cctgcacgta cagtgtggcc
     1921 ggggaattcc ccccggttct ttcgtacatt ccgatgatca tggccgcaag atccttctcc
     1981 cggccgggga agaaaacctt ctttccgtca ctgccggaag cggtgcggtc aaaaaaggcg
     2041 aacactccct tgacatccgg cggcacgccg cagggatctc ccacccagat cctgggcagg
     2101 ggcagtccct tg
//
LOCUS       DAOTHD010000369         2107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_62481_length_2107_cov_3.203704, whole genome shotgun sequence.
ACCESSION   DAOTHD010000369 DAOTHD010000000
VERSION     DAOTHD010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62481_length_2107_cov_3.203704"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="P5219_06885"
     CDS             complement(<1..1048)
                     /locus_tag="P5219_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRX26518.1"
                     /translation="MNGDLPGTQGGVVFKEVTKTYPDGTNAVDRLDLEIRSGGLVSLI
                     GPSGCGKTTTLKMINRLEEPTSGRITVEGEDILRCDPVELRRRIGYVVQEIALFPHMT
                     VSENIAVVPSLLGWPAKKIRSRVDELLAMAGLPPEKYRFRLPDQLSGGQKQRIGVLRA
                     LASDPKVILMDEPFGALDPISREVLQNELVSLQKNLKKTIVFVTHDMGEAIRISDRIV
                     ILRKGRVEQEGSAEDIQYSPKNDFVRSFIGEDRLSRMSPDDPAESIVEEPWGTAAPWE
                     GAAGVLERMEENDREFIQVVDPKSGQWLGMALLGRLRRAASAGETVMRGIYRERKLYA
                     GDATLRDAAEMLADR"
     gene            complement(1045..>2107)
                     /gene="hisC"
                     /locus_tag="P5219_06890"
     CDS             complement(1045..>2107)
                     /gene="hisC"
                     /locus_tag="P5219_06890"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRX26519.1"
                     /translation="KTVEEVRRELSLERIVKLGSNENPWGPFPAARAAMAEEIVRLNT
                     YPDVSFRELKGLIARQSGLEPEWVALSHGAEGMLQTLGKVFLDPGDEVVLPKGTYGLY
                     REISLVMGAVLREIPLGDDFSMDLEEMRKAITGKTKLVWLNNPNNPTGLALPPEKVEG
                     FVRALPEGTWVVLDEAYGEFAAPGSMPGTKALLEERRNLVAVRTFSKAWGLAGARIGY
                     ALARPELVTVIDTVSEPFNANRTAIAGASAALREDGEAFRKALDAIVSERERVSRSLG
                     AMGLRVLPSSTNFIFFTLPLDASEVSRMLLERGVIVRPCGGWGFPRSIRVTVGTAEDN
                     SFFLEALGQVLAERKGERE"
BASE COUNT          340 a          717 c          649 g          401 t
ORIGIN      
        1 ccctgtcggc gagcatctcc gccgcatccc ggagggtggc gtcgcccgcg tagagctttc
       61 tttcccggta gatgcccctc attaccgtct cacctgcgga cgccgcccgg cgcagccgtc
      121 cgagaagggc catgcccagc cactggccgc ttttgggatc caccacctgg atgaactccc
      181 ggtcgttctc ctccatgcgt tccagcacgc cggcggcccc ttcccaggga gcggccgttc
      241 cccagggctc ctccacgatg gactccgccg ggtcgtcggg ggacatgcgg gagagcctgt
      301 cctcgccgat gaagctccgg acgaagtcgt ttttcgggga atactgaatg tcctccgccg
      361 agccctcctg ctccaccctc cccttccgca ggatcacgat cctgtcggag atgcggatgg
      421 cctcccccat gtcgtgggtg acaaagacaa tggttttctt cagattcttc tggagggaga
      481 ccagttcgtt ctggagcact tccctgctta tgggatccag ggcgccgaag ggttcgtcca
      541 tgaggatgac cttcgggtcc gacgccaggg cccggagcac gccgatccgc tgcttctgtc
      601 ctccgctgag ctgatcggga agacggaacc ggtatttttc cggcggaagc cccgccatgg
      661 cgagaagctc gtccaccctg gaccggattt tcttcgccgg ccaccccagc agggagggca
      721 caacggcgat attctccgac accgtcatgt gagggaacag ggcaatctcc tgcaccacgt
      781 acccgatgcg cctccggagc tccacgggat cgcaccggag aatatcctcc ccctccacgg
      841 tgatccttcc cgaggtgggt tcctccagcc tgttgatcat cttgagggtg gttgtctttc
      901 cgcagcccga cggaccgatg agactgacca gcccgccgct ccgtatttcc aggtccaggc
      961 ggtccacggc attggtgccg tcgggatagg tcttcgtcac ctctttgaaa accacgccgc
     1021 cctgtgtacc tggcagatct ccgttcattc ccgttccccc tttctctccg cgagcacctg
     1081 tccgagagct tccaggaaga aggaattgtc ttccgccgtt ccgacggtca cccggatgga
     1141 gcggggaaat ccccagccac cgcagggacg gacgatgaca ccccgctcca ggagcatacg
     1201 ggacacctcc gaggcgtcca gggggagggt gaagaaaatg aaattggtgc tcgacggcag
     1261 gacccggagc cccatggccc caagggagcg ggacacccgc tcccgctccg agacgatggc
     1321 gtccagggcc ttccggaagg cttcgccgtc ctcccgcagg gccgccgagg ctcccgcgat
     1381 ggccgtccgg ttggcgttga agggctcact caccgtgtcg atgaccgtga ccagctccgg
     1441 acgggcgagg gcgtagccga tccgtgcccc ggcgaggccc caggccttgg agaaggtcct
     1501 caccgccacg agattccgcc tttcttcgag gagagccttc gtgccgggca tgcttccggg
     1561 ggcggcgaac tcgccgtagg cttcgtcgag gacaacccac gtcccctcgg ggagggcgcg
     1621 aacgaagccc tccacctttt cgggaggcag ggccagcccc gtggggttgt tggggttgtt
     1681 cagccagacg agcttcgttt tgccggttat cgcctttctc atttcctcaa ggtccatgga
     1741 gaaatcgtct ccgaggggaa tttcccggag gacggccccc atgacgaggg atatttcccg
     1801 gtagagtccg taggttccct tcggcaggac gacctcgtcg cccgggtcga ggaagacctt
     1861 ccccagggtc tggagcatgc cctccgcccc gtgggacagg gcgacccatt cgggttccag
     1921 gccggactgc cgggcgatga gccctttcag ctcgcggaag gaaacgtcgg gataggtgtt
     1981 cagcctgacg atctcctcgg ccatggccgc ccgggccgcc gggaaggggc cccaggggtt
     2041 ctcgttggac cccagcttca ctattctctc gaggctgagc tcccgccgga cctcctccac
     2101 agttttg
//
LOCUS       DAOTHD010000370         2105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_62575_length_2105_cov_2.971707, whole genome shotgun sequence.
ACCESSION   DAOTHD010000370 DAOTHD010000000
VERSION     DAOTHD010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62575_length_2105_cov_2.971707"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="P5219_06895"
     CDS             complement(<1..499)
                     /locus_tag="P5219_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26520.1"
                     /translation="MHTYRNPADISRLLSDRTGDPGRVFLVPSNRDRPLLLDMLSAEG
                     CSAPEGLSWNRIRERNVWIWDDLYRALLEAGTEEKPRAQIDPPDHWLLLRSIISSLRE
                     RFAESLPPGVFSEGFLDLAGGAVRELLREDVPPETLAGSLGCAGCPQDEGCSRLDDEL
                     GILCRI"
     gene            729..971
                     /locus_tag="P5219_06900"
     CDS             729..971
                     /locus_tag="P5219_06900"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRX26521.1"
                     /translation="MIITASKARANLYRLIDHVTETEEPVYITGKRGNVVVLSEDDYR
                     DIQETLALAAVPGMRDKILSGMAAPLSECVEIDEDA"
     gene            964..1230
                     /locus_tag="P5219_06905"
     CDS             964..1230
                     /locus_tag="P5219_06905"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HRX26522.1"
                     /translation="MHDGLWRVFYTKEALKDRERVIERGWGEKIRSIERALRENPYAP
                     PGEKLVGEFKGAYSKRINRQHRVVYQILEEDRAVKVVSAWSHYE"
     gene            1393..1563
                     /locus_tag="P5219_06910"
     CDS             1393..1563
                     /locus_tag="P5219_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26523.1"
                     /translation="MRKGRIALFLALLLVFSALPATAADGPVLKLAPGVAEKLQTIGP
                     ELSLDWGVYKNT"
     gene            1627..1758
                     /locus_tag="P5219_06915"
     CDS             1627..1758
                     /locus_tag="P5219_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26524.1"
                     /translation="MTDETGGVTILINKAITFEDNIVLVEHFTYRGRDVFICRTAKY"
     gene            1781..>2105
                     /locus_tag="P5219_06920"
     CDS             1781..>2105
                     /locus_tag="P5219_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase"
                     /protein_id="HRX26525.1"
                     /translation="MKNLGEMLLRTEPFSEIVLGNTALARAMIESGTEVVTSYPGSPT
                     PEIASALLSVPGEKRPFYFQFSTNEKVATEVACGASLNGHPSCVFFKSVGLNVAADSF
                     VQLSLL"
BASE COUNT          465 a          602 c          662 g          376 t
ORIGIN      
        1 agatccggca gaggatacct aactcgtcgt ccagccggga gcatccctcg tcctgaggac
       61 accccgcgca ccccagcgaa ccggccaggg tctccggggg cacgtcctcc cgcagaagct
      121 cccggaccgc ccctccggca aggtcgagga acccttccga aaagactccc ggcggcagag
      181 attcggcaaa ccgctcccgc aatgagctaa tgatgctcct cagaagcagc cagtggtccg
      241 gcgggtcgat ctgagcccgg ggcttctctt ccgtccccgc ctccagaaga gcccggtaga
      301 ggtcgtccca gatccagacg ttccgttccc ggatcctgtt ccaggaaagg ccctccggag
      361 cggaacaccc ttcggcggaa agcatgtcca ggagaagggg tctgtcgcgg tttgacggta
      421 ccaggaacac ccgtccggga tcaccggtcc ggtccgacag aagacgggaa atatccgcgg
      481 gattgcggta ggtgtgcatc agcagggcta tcgcctcctc actatacgga agaagtgtag
      541 cacatttcaa tccggggaaa gatccggaag agggagaggt caaccgccgg cggagccgca
      601 ccaacggggg atcatcagaa atccgggaga acggatcatg ccttcgccca aggagaggaa
      661 tggagagagt acgtgcttga ctcgtacgag atgacgtact atgataaatg cgtcagggag
      721 gtgatgtcgt gatcatcacg gcgagcaagg cgcgggcaaa tctctacagg ctgatagacc
      781 acgtaacgga gacggaagaa ccggtctaca tcaccggaaa gcggggcaac gtggtggtcc
      841 tgtcggagga cgactaccgg gacatccagg agacccttgc tcttgcggcg gtacccggaa
      901 tgcgggataa aattctctca ggcatggccg ctcccctgtc cgagtgtgtc gaaatagacg
      961 aagatgcatg acgggctgtg gcgggtgttc tacacgaagg aggccctgaa ggacagagag
     1021 agggtcatcg aacggggctg gggcgaaaag atccggtcca tagagcgtgc cctccgggag
     1081 aatccatacg cgcctcccgg ggagaaactg gtgggagagt ttaagggggc ctactcgaag
     1141 aggataaacc ggcagcatcg ggtggtctat cagatacttg aggaagaccg ggcggtcaag
     1201 gtggtcagcg cctggagtca ttacgaatag cgggaataac gatggaaaag ccgcatccca
     1261 agggatgcgg ctttttgggg cctgtcggaa ggcagggccg gctggtttcc tatccgccga
     1321 aggactgaat gcgggttctt cccgtcatgg tacaatgcag gcataaccat gataccttga
     1381 ggaggtatga ccatgagaaa agggagaatc gcactgtttt tggctctgct gctcgttttt
     1441 tcggcgctgc ccgcaacggc cgccgatggt cctgtcctga agctggctcc gggagtggct
     1501 gaaaaactgc agaccatcgg gccggagctg tccctcgact ggggcgtcta caagaacacc
     1561 tgaggggagg agccatcggc tctcgtcgag agcggcaggt ccctcgggct ggaacactac
     1621 attccggtga ctgacgaaac aggcggggtt accatcctca tcaacaaggc gattaccttc
     1681 gaggacaata ttgtcctcgt ggagcacttc acctaccgcg gaagggatgt tttcatctgc
     1741 aggacggcga agtactgacc accggaaagg aggacgggac atgaaaaatc tcggcgaaat
     1801 gctgctgcgg accgagccct tttccgagat cgttctcggg aacaccgccc tggcccgggc
     1861 catgatcgag tcggggacgg aggttgtcac gtcgtatccc ggttccccca cccctgaaat
     1921 cgcatcggcg cttctctccg ttcccgggga gaagcgccca ttctatttcc agttctccac
     1981 caacgaaaag gtcgctacgg aggtggcctg cggcgcctcc ctcaacgggc acccctcctg
     2041 cgtcttcttc aagagcgtgg ggctcaacgt ggccgccgac tccttcgtcc agctatccct
     2101 cctgg
//
LOCUS       DAOTHD010000371         2105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_62578_length_2105_cov_2.787317, whole genome shotgun sequence.
ACCESSION   DAOTHD010000371 DAOTHD010000000
VERSION     DAOTHD010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62578_length_2105_cov_2.787317"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /gene="ribA"
                     /locus_tag="P5219_06925"
     CDS             <1..759
                     /gene="ribA"
                     /locus_tag="P5219_06925"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HRX26526.1"
                     /translation="LPPAGVICEIMNEDGTMARLPDLVTFAGKNGLKIISIEDLIAYR
                     SKREKLVEKIAKVELPTSHGFFTAHAYRNLNDDNLDHVHMALVKGAVEGKENVLVRVH
                     SECLTGDVFGSLRCDCGPQLAQAMEMIEAEGQGILLYMRQEGRGIGIAEKLKAYQLQE
                     KGLDTVEANVALGYPADLRDYGIGAQILQDLGVGSIRLITNNPRKVVGLQGYGLEIVE
                     RIPLVIPPNRYNERYLGTKESKLGHLFHVRDFLS"
     gene            784..1275
                     /gene="ribE"
                     /locus_tag="P5219_06930"
     CDS             784..1275
                     /gene="ribE"
                     /locus_tag="P5219_06930"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRX26527.1"
                     /translation="MKIIEGKLTGAGLRFCIVVSRFNSLFSEKLLEGAKDMLLRHDVS
                     HQAIDVIRVPGAWELPLVAKEAALSGKYDAIIALGAVIRGDTPHFEYVSAEMSKGLAS
                     VAMAERIPVSFGVLTTDTMDQAVARSGSKGGNKGAEAALAAIETANIMNELRTGKEKE
                     DHA"
     gene            1268..>2105
                     /gene="serS"
                     /locus_tag="P5219_06935"
     CDS             1268..>2105
                     /gene="serS"
                     /locus_tag="P5219_06935"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRX26528.1"
                     /translation="MLDIRWIRSNADEVRQFLADRNNDLDIGPLLGLDEERRLLLTET
                     EELKARRNEGSRKVGAARARGEDASAAMEEMRVIGDRIKDIDARISEIDEKISDMLLS
                     IPNRPHDSVPVGKDENDNPEVRRWGEPKQFSFEPKPHWDLGEALGILDFEKGASLAQS
                     RFTVLKGLGARLERALLNFMLDLHTEKHGYLEVQPPFMVSSKTMRGTGQLPKFADDLY
                     KCENDDLWLIPTAEVPLTNLHAEEILDEGQLPLYYTAYTPCFRREAGAYGRDVRGMLR
                     QHQ"
BASE COUNT          461 a          607 c          672 g          365 t
ORIGIN      
        1 ctgcctccgg cgggggtaat ctgcgagatc atgaacgagg acggcaccat ggcccgcctg
       61 cccgacctgg tgaccttcgc gggaaagaac ggtctgaaga tcatttccat cgaggacctt
      121 atcgcctacc ggagcaaacg ggagaagctg gtggaaaaga tcgcgaaggt tgagctgcca
      181 acctcccacg gttttttcac tgcccatgcc taccggaacc tgaacgacga caacctcgat
      241 cacgtgcaca tggccctcgt gaaaggggct gtggaaggga aggagaacgt tcttgtgagg
      301 gtccattccg aatgcctcac gggggacgtg ttcggttccc tgaggtgcga ctgcggtccc
      361 cagctcgccc aggccatgga aatgatcgag gccgaggggc aggggattct tctctacatg
      421 cgccaggaag gcaggggcat cgggatcgcc gaaaagctca aggcctacca gctccaggag
      481 aagggactcg acacggtgga ggcgaacgtg gccctgggat accctgcaga cctgagggat
      541 tacggcatcg gtgcccagat ccttcaggac cttggtgtcg ggagcatccg cctcatcacc
      601 aacaatccga ggaaagtggt ggggctccag ggatacggcc ttgagatcgt ggagcggatt
      661 cccctggtta tcccgccgaa ccgctacaac gaacggtacc tcggcacgaa agaatccaag
      721 ctcggacatc tttttcacgt ccgggacttc ctgagctgac acaggctgaa aggagcggtt
      781 gaaatgaaaa tcatcgaggg aaaactcacc ggagccggac tgcgtttctg catcgtcgtg
      841 tcgaggttca acagcctctt cagcgaaaag ctgctcgagg gggcgaagga catgctgctg
      901 cggcatgatg tgagccacca ggccatcgat gtcatacggg tgcccggtgc gtgggagctg
      961 ccgcttgtag ccaaggaagc ggccctgagc ggaaagtatg atgccatcat cgccctgggg
     1021 gcggtcatcc ggggagatac gccccacttt gaatacgtct ccgccgaaat gtccaaggga
     1081 ctggcatcgg tggccatggc cgagcggatt cccgtgtcct tcggggtgct gaccacggat
     1141 accatggacc aggccgtggc ccggtcaggc agcaaggggg gcaacaaggg ggccgaagcg
     1201 gcccttgcgg ccatcgaaac cgccaacatc atgaacgagc ttcgcaccgg aaaggagaag
     1261 gaagaccatg cttgacatcc gatggatccg ctccaacgcg gatgaagtga gacagttcct
     1321 tgcggacagg aacaacgatt tggatatcgg gccgcttctc gggcttgacg aggagcgccg
     1381 tctgctgctc acggagactg aggagctgaa ggcccgcagg aatgaaggat cccggaaggt
     1441 gggggccgcc agggccaggg gcgaggatgc ctccgccgcc atggaggaga tgcgggtcat
     1501 cggcgacagg ataaaggaca tcgacgcccg catttccgag atcgacgaaa agatttcgga
     1561 tatgcttctt tccattccga accgccctca tgattccgtt cccgtgggga aggacgagaa
     1621 cgacaaccct gaagtccggc gctgggggga gccgaagcag ttttccttcg agcccaagcc
     1681 tcactgggat ctcggcgagg ccctgggcat tctggatttc gagaaggggg catcccttgc
     1741 ccagagccgc tttacggttc tgaaggggct cggcgcccgg cttgagcggg ccctgctgaa
     1801 cttcatgctc gaccttcaca cggagaagca cgggtacctg gaagtacagc cgcccttcat
     1861 ggtgagttcg aaaaccatgc ggggaacggg acagcttccc aagttcgccg atgacctcta
     1921 taaatgcgag aacgacgacc tctggctcat ccccaccgca gaggtgcccc tcaccaacct
     1981 ccacgccgag gaaattctcg acgaggggca gcttccgctg tactacacag cctatacccc
     2041 ctgtttccgc agggaggccg gggcctacgg ccgggacgtg cggggcatgc tcaggcagca
     2101 ccagt
//
LOCUS       DAOTHD010000372         2097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_62890_length_2097_cov_2.354065, whole genome shotgun sequence.
ACCESSION   DAOTHD010000372 DAOTHD010000000
VERSION     DAOTHD010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62890_length_2097_cov_2.354065"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1473)
                     /locus_tag="P5219_06940"
     CDS             complement(97..1473)
                     /locus_tag="P5219_06940"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRX26529.1"
                     /translation="MTEQHSGEFRNGSGEGMRKALSYRWVVWGVMVFAFMVVFFHRFA
                     AAVVKDDVTREFSLSATAFGAMASMYFYAYMLMQIPVGFLADSLGARITVSLGMFLAG
                     AGSVIFGFAPSSLWLFIGRFLVGIGVSTVFVSIMKIQSQWYRDSEFATISGATTLVGN
                     AGGIFSQGPLALLISVVSWRTGFVAIGLLTVAIAALCFRFIRNRPQDIGLPPVNEREI
                     ARANLRPEPFSAVSGLKSVLSVRGILPATFFYLFNQAGFFALISTWGIPWMVNAYGLS
                     VQEASSYSVILIFGIMAGGFITGWISDRLGRRKAPMLVSSLLHMVLWAVILLSENGMP
                     PLGTLKILFFLLGITNTAFVLSWSVAKELTSEKFTGLAISILNAAGFLSIAVCTSVMG
                     WVLDLFSDLSPAGAYRMAFILPLVSATLSVISVLFIPETGTGKKRGRQTESPVKENEV
                     SSNGKAFY"
     gene            1633..>2097
                     /locus_tag="P5219_06945"
     CDS             1633..>2097
                     /locus_tag="P5219_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRX26530.1"
                     /translation="MIRISNIRIQLGERSLFQDLSWTVLPGSRTGLVGNNGAGKTTLL
                     RIMAGETTVDSGTVTVPKGLRIGYLPQDLVELPDVPVLSFLRSRTGLAAAEEELAACA
                     GRLAGLPPESPDHERELNRHDLLLKRFEGLDGYAFDAMAGKILSGLGFSQEDM"
BASE COUNT          530 a          551 c          561 g          455 t
ORIGIN      
        1 aggattttta cattgatgtc aaggtttttt tcattccggt aacggtttgc gtccattttt
       61 tctcttctgc agcctttttt tttccgtgaa aagacattaa taaaaagcct ttccattaga
      121 tgaaacttca ttctctttca ctggtgactc agtctgtctt ccccgttttt ttccggttcc
      181 ggtttcaggg atgaaaagaa ccgaaataac ggaaagggtt gccgacacaa ggggaagaat
      241 gaaggccatt ctatatgctc ccgcaggaga aaggtctgaa aaaaggtcga gcacccagcc
      301 catcacggag gtgcagacgg cgatggaaag aaaaccggcg gcgttgagaa ttgaaatggc
      361 aagcccggtg aacttttcag atgttagctc cttggccacc gaccatgaga ggacgaaggc
      421 cgtattcgtt atgccgagta agaaaaaaag aatcttcaac gtcccgaggg gaggcattcc
      481 gttttccgac agaaggatca cagcccacaa aaccatgtga agcagcgaac tgactagcat
      541 gggagccttt ctccttccga ggcggtcgga aatccagcct gtgatgaatc cccccgccat
      601 tatgccgaag ataaggatca cgctgtagga agacgcttcc tggactgaaa gcccgtaggc
      661 gttcaccatc cagggaatcc cccaggtgct gatgagagcg aaaaaaccgg cctggttgaa
      721 gagatagaaa aaggtcgcag gaaggatacc cctgaccgaa aggacactct tcagtcccga
      781 gactgcagaa aagggttccg gccggaggtt tgccctggcg atttcccttt cgtttaccgg
      841 gggaagtcca atatcctggg gcctgttccg gatgaaccgg aaacacaggg ccgcaatcgc
      901 cacggtcagg agtcctatgg ccacaaatcc ggttctccat gaaacaaccg agatgaggag
      961 ggccagcggt ccctgggaga aaatgccgcc ggcgttcccc acaagggtcg ttgctcccga
     1021 aatggtggcg aattcactgt cgcggtacca ttgggactgg attttcatta tggagacaaa
     1081 tacggtggac acaccgatgc cgacgagaaa tctcccgatg aagagccaga gggaggacgg
     1141 agcgaatccg aagatgaccg atccggctcc ggcaaggaac atcccgaggg agaccgtgat
     1201 tcttgctccc agggaatcgg cgagaaatcc caccggtatc tgcatgagca tgtaagcgta
     1261 gaaatacatg gaggccatgg cgccgaaagc tgtagcgctg agtgaaaatt ctctcgtcac
     1321 atcatccttt accactgcgg cggcaaaacg atgaaagaac accaccatga aagcgaaaac
     1381 cataacgccc cacacaaccc aacggtagga gagggccttc ctcatcccct ccccgcttcc
     1441 gtttctgaac tctccggaat gctgctccgt cattttctct cctcttttct ccgggttctt
     1501 gtgcggactg tgatccgccg gagtgatccg taaaaagaaa taataccatt gaattccgtt
     1561 ttttcctctt ttttccccgg aaaaatcacc gtataatcag gttcattgag aaacagagaa
     1621 ggaggcaggc tcgtgatccg gatttccaac atccgaattc agctgggaga gcgatccctc
     1681 ttccaggatc tttcgtggac cgttcttccg ggatcccgta ccggcctcgt ggggaacaac
     1741 ggcgcaggaa aaacaacgct gctcaggata atggcagggg agacgactgt ggactccgga
     1801 acggtgactg tccccaaggg actcaggatc ggctatcttc cccaggacct ggtggaactg
     1861 cctgacgtgc cggttctttc cttcctgcgt tcaagaacgg gcctcgcggc agcggaagag
     1921 gaactcgcag catgcgccgg acgactggcc ggactacccc ccgaatcccc tgatcatgaa
     1981 cgggaactca atcgtcacga ccttcttttg aaacggtttg agggcctgga cggatatgct
     2041 tttgatgcca tggcggggaa aattctttca ggtctgggct tttcccagga ggacatg
//
LOCUS       DAOTHD010000373         2087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_63295_length_2087_cov_1.666339, whole genome shotgun sequence.
ACCESSION   DAOTHD010000373 DAOTHD010000000
VERSION     DAOTHD010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63295_length_2087_cov_1.666339"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1691
                     /locus_tag="P5219_06950"
     CDS             <1..1691
                     /locus_tag="P5219_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX26531.1"
                     /translation="EWSATGPGTIGHASWDTASVTLNGTGSLAVTVLAKVGGKEAGRA
                     EVILPVTVAKSRIQAAREYQAGRNEWNAARRDRAVIAMERAASLDPGNPAIERELARM
                     KGEAEKSRAEEARKRAVDVWNQAAELQKKEKYEDALEKYREGLKISDEKAVRDHVKKL
                     EDFIADRKRRQEQAEADARARAEAVWRRAAELQKGKRYEDALEKYREGLAIFPDPVVE
                     EHVRKLEDYIARMKNAAEAKKPAEAPSSGGGKDPGAETPAKKPGTPPADTEQHWRGWK
                     SFTLAGIRYEIPAGGFQSVKSSSGKENGKYPFANYDYYMNGKRVAVVRIGTLAPGLTD
                     GKIKGLYTGKTANRIFDSLSGEGTVTLGGRKAYYLQGNAPGVTRKFFFIADGAEGGMG
                     AYLHVVAGSGSGWKKYAPMFGEIAGRMSFTAGKTPPQSGADKGAGTLPAGWKSVTIGN
                     VTFGIPSTWNGKTMKEHDVESLHVYWQGSFDNPVHGVSGGVTKKYGEAKKELRGARTV
                     SLGGAKVLRADEDGAVNLLFPPLGGNRGVALVLFRGKGGKQATLEAILKTVTVQ"
     gene            complement(1762..>2087)
                     /locus_tag="P5219_06955"
     CDS             complement(1762..>2087)
                     /locus_tag="P5219_06955"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26532.1"
                     /translation="AMGRPKDALKAYYWFDESAKNGFIDAMVNLGVLYEKGDGAVFSR
                     EKAFQWYSKAAEKGSSLGMAYLGEVYYQGRASRRMRKRVSASWSRRLGRTSHTPSGCS
                     IKSTL"
BASE COUNT          476 a          592 c          702 g          317 t
ORIGIN      
        1 gggaatggtc ggccacgggg cccggaacaa tcggccatgc gtcatgggac actgcctcgg
       61 tgaccctgaa cggaacaggg tccctggccg tcacggtcct ggcgaaagta ggggggaaag
      121 aagcgggcag ggcggaagtg atcctccccg tgaccgtggc aaagagcaga atccaggcgg
      181 cgagggagta ccaggccggg cggaacgagt ggaacgccgc ccgccgggac cgggcggtca
      241 tcgccatgga acgggcggca tccctggatc cgggcaatcc cgccatcgag cgggaactgg
      301 cgcggatgaa gggcgaggcg gaaaagtccc gggccgaaga agcccggaag cgggccgtgg
      361 atgtctggaa tcaggccgcg gaactccaga agaaggaaaa gtatgaagat gcccttgaaa
      421 aatacaggga gggcctgaaa atcagcgatg aaaaggctgt ccgtgatcac gtgaagaagc
      481 tcgaggactt catagccgac aggaagcgca gacaggagca ggccgaagcc gatgcccggg
      541 cccgggcgga ggccgtctgg cgcagggcgg cggagctgca gaagggaaaa cggtacgaag
      601 atgccctgga gaaatacagg gaaggccttg ccatcttccc cgacccggtg gtggaggagc
      661 acgtccggaa gctggaggac tatatcgcca ggatgaagaa tgcggcggaa gcaaagaaac
      721 ccgcagaggc tccttcttcc ggcggaggga aggatccagg ggcggaaacg ccagcgaaga
      781 aacccggaac tccaccggcc gacacggagc agcattggag aggatggaag agcttcaccc
      841 tcgccggaat ccggtacgaa atcccggcag gaggcttcca gagcgtgaaa agctcctcgg
      901 ggaaggaaaa cggaaaatac cccttcgcaa actacgatta ttacatgaac ggcaagcggg
      961 tggccgtcgt ccgcatcggc accctggcgc cggggctcac cgacggcaaa atcaaggggc
     1021 tctataccgg aaagaccgcc aaccggatct tcgactccct ctccggcgag ggaacggtga
     1081 ccctgggcgg ccggaaggcc tactacctcc aggggaacgc ccccggagtg acccggaagt
     1141 tcttcttcat agccgacgga gccgagggcg gcatgggagc ctaccttcac gtggtggccg
     1201 gcagcgggag cggctggaaa aagtacgccc ccatgttcgg ggagatcgcc ggaagaatgt
     1261 cgttcacggc gggaaaaact cctccgcaga gcggggcgga caagggggcg ggaacgctgc
     1321 ccgcaggctg gaaatccgtg accatcggga acgtcacctt cggcattccc tctacctgga
     1381 acgggaagac aatgaaggaa catgacgtgg aatcgcttca cgtctactgg cagggaagct
     1441 tcgacaaccc ggtccacggc gtctccggag gggtgacgaa gaagtatggg gaggcgaaaa
     1501 aggagctccg cggtgcccgg acggtctccc tcggaggggc gaaggtgctc cgggcggacg
     1561 aggacggagc ggtgaacctt ctctttccgc cccttggcgg caacaggggc gtggccctcg
     1621 tcctcttccg gggaaagggg ggcaaacagg cgaccctgga ggccatattg aagactgtca
     1681 cggtacagta aaacaacggg gccccttcag gggccccgtt gtttcagaac gattatttca
     1741 cgttccggta cttcatgtac ttcacagagt tgattttata gagcagccgc tgggcgtatg
     1801 cgaggttctt cccagccgcc tgctccagga ggccgatacc ctttttctca tccttcggga
     1861 cgcccggccc tggtaataga cctcgccgag gtaggccatg ccgagggagg atcccttttc
     1921 agcggccttc gaataccact ggaaggcctt ctccctgctg aacacggctc catcaccttt
     1981 ttcatagagt actccgaggt tcaccatggc gtcgatgaag ccgttcttcg ccgactcgtc
     2041 aaaccagtaa taggctttca gggcgtcctt gggccgtccc atggcct
//
LOCUS       DAOTHD010000374         2086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_63315_length_2086_cov_2.991137, whole genome shotgun sequence.
ACCESSION   DAOTHD010000374 DAOTHD010000000
VERSION     DAOTHD010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63315_length_2086_cov_2.991137"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..977
                     /locus_tag="P5219_06960"
     CDS             93..977
                     /locus_tag="P5219_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRX26533.1"
                     /translation="MSAKKEGFFKSEFFHNFVRSPGALAGLFIVTFFVVLVTAGPLFT
                     PQNPYDIGSLDLMDAFKPPVWMEGGDPAFVLGTDDQGRDLLSAMVYGSRISLFIGLSV
                     VVLSCVLGTFLGLVAGYFGGRLDSLLMRVVDIQLSFPAMLVALFIMAAFGRGLGKLVT
                     ALTIVGWVLYARTVRGSVLVEKKKEYVDAARLIGLSPFPIILRHVLPNILTPLIVIAT
                     IHVGTVILTEATLSYLGVGVPVTRPSLGLLVKNGYDVLFSGLWWSSVFPGMFIMLLVF
                     GINLLGDFLRDEFNPRLK"
     gene            987..1988
                     /locus_tag="P5219_06965"
     CDS             987..1988
                     /locus_tag="P5219_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX26534.1"
                     /translation="MSEYLLEVRNLQTRFELFRGTVKAVDGVDFTLREGEILGLVGES
                     GGGKSVTGFSLLGLIDPPGRIAAGEVIFRGENLLEKSEEEMRRIRGKEISMIFQDPMT
                     SLNPLQSIGRQIGEMLALHTPLSPADRRKRSIELLREVGIPNPEDRLENYPHQFSGGM
                     RQRVVIAIALAANPKLIIADEPTTALDVTVQAQILALMENLVKRFGSALILITHDLAV
                     VSEMTHRVAVMYCGRVVEEGPTRELVRHPFHPYTKGLLESIPRLSGERSRRLPQIPGM
                     VPSLFDLPPGCSFAPRCGYATDLCRHEAPRMRAVAPGRTVSCHNPVQGGEEAEGHGR"
BASE COUNT          365 a          687 c          632 g          402 t
ORIGIN      
        1 atgatcgtgg cggccatgtt cgtcctgatc aactttatcg tggacatcgt ctataccctg
       61 atcgatcccc gaatcgacct gaggtgaccg gaatgagcgc aaaaaaagaa ggatttttca
      121 aaagcgagtt ctttcacaat ttcgtccgga gccccggtgc cctggcaggg ctcttcatcg
      181 tcacgttttt cgtggtcctt gtgaccgcgg ggcccctgtt caccccccag aacccctacg
      241 acatcggcag cctggacctc atggacgcct tcaaaccccc tgtctggatg gagggcgggg
      301 acccggcctt tgtcctcggc acggacgacc aggggcggga cctgctgagc gccatggtct
      361 acggcagcag gatctccctg ttcatcggcc tgtcggtggt ggtcctctcc tgcgttctcg
      421 gcacctttct cggcctcgtg gcgggctact tcggcggcag gctcgacagc ctcctcatgc
      481 gggtggtgga catccagctc tccttcccgg ccatgctggt ggccctcttc atcatggccg
      541 ccttcggccg gggcctcgga aagctggtga ccgccctcac catcgtgggc tgggtgctct
      601 acgcccggac ggtcaggggc tccgtgctgg tggagaagaa aaaggaatac gtggacgccg
      661 ccaggctcat cggcctctct cctttcccca tcatcctgcg gcacgtgctg cccaacatcc
      721 tgacccccct catcgtcatc gccacgatcc acgtgggaac ggtgatcctc acggaagcca
      781 ccctcagcta cctcggggtg ggcgtacccg tgaccaggcc ctccctgggc cttctcgtca
      841 agaacggcta cgacgttctc ttcagcggcc tctggtggtc gtcggtcttt ccggggatgt
      901 tcatcatgct cctggtcttc ggaatcaatc tcctggggga ttttctccgc gacgagttca
      961 atccccgcct gaaatagggg tatcccatgt ctgaatacct tcttgaagtc agaaatctgc
     1021 agacccgctt cgaactcttc cggggcacgg tgaaggccgt ggacggggtg gacttcaccc
     1081 tcagggaagg ggaaattttg gggctggtgg gggaatcggg gggcggaaag tccgtcaccg
     1141 gcttctccct cctcggcctc atcgatcctc ccgggcggat cgccgcaggg gaagtgatct
     1201 tccgggggga gaacctgctg gagaagtcgg aggaggagat gcgccgcatc cgggggaagg
     1261 agatttccat gatcttccag gaccccatga cctccctgaa ccccctgcag tcaatcggcc
     1321 ggcagatcgg cgagatgctg gccctccata cccccctctc cccggcggac cgcaggaaga
     1381 gaagcatcga gctcctcagg gaggtgggca tccccaaccc cgaggacagg ctcgagaact
     1441 acccccacca gttttccggg ggcatgcggc agcgggtggt catcgccatc gcccttgccg
     1501 cgaacccgaa actcatcatc gccgacgaac ccaccaccgc cctggacgtg acggtgcagg
     1561 cccagatcct tgctctcatg gaaaatctcg tgaaacgctt cggctccgcg ctcatcctca
     1621 tcacccacga cctcgccgta gtctccgaga tgacccacag ggtggccgtc atgtactgcg
     1681 gcagggtggt ggaggaagga cccacgaggg aacttgtacg gcatcctttc catccctaca
     1741 cgaaggggct gctggagtcc atccccaggc tgtccgggga aagaagccgg aggctccccc
     1801 agatacccgg catggtgccg agccttttcg acctcccgcc gggatgttcc tttgcccccc
     1861 ggtgcgggta tgccacggat ctctgcagac acgaggctcc ccggatgcgt gccgtcgccc
     1921 ccgggcggac ggtgagctgc cacaaccccg tacagggcgg agaggaggcg gaaggccatg
     1981 gccggtagcg gacggaatgc gcttctcgag gtcacggaac tcgtccagag tttcagccag
     2041 ccccggggca tcctggaccg gctggcggga aggccggtga aaaccg
//
LOCUS       DAOTHD010000375         2086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_63320_length_2086_cov_2.654357, whole genome shotgun sequence.
ACCESSION   DAOTHD010000375 DAOTHD010000000
VERSION     DAOTHD010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63320_length_2086_cov_2.654357"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="P5219_06970"
     CDS             <1..187
                     /locus_tag="P5219_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HRX26535.1"
                     /translation="RRMRLSISALEEPSQRRPRDDDRKKGRQDRREHDNQPKPVMNDG
                     EASVTIGELLRNSMQEE"
     gene            266..772
                     /gene="def"
                     /locus_tag="P5219_06975"
     CDS             266..772
                     /gene="def"
                     /locus_tag="P5219_06975"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRX26536.1"
                     /translation="MDSLYLRVYPDPVLRKETQSVTDFGDGFKDLVADLVRLMHQHDG
                     VGFAAPQAGVSLKLAVVFYQGELHVLANPVLVSSAGEQDGEEGCLSFPGIFGNVKRPL
                     KAVVRHFDINGAEKVTEAEGFLARAFLHEMDHLEGRLLIDRFSPLKRSMAKKKLLRAE
                     AEREAEKG"
     gene            769..1722
                     /gene="fmt"
                     /locus_tag="P5219_06980"
     CDS             769..1722
                     /gene="fmt"
                     /locus_tag="P5219_06980"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRX26537.1"
                     /translation="MISLSSGETRAALWFFGAGRFAARCLSVLSREISFDLVVTSPPS
                     VGGRGLKELPSPVEEACLELGITPHRSGSAGKDEVLLSALVETPPLAILVVDFGQKIP
                     EPFLSEPAGGCLNIHPSLLPLYRGAAPVQRSLLNGDSVTGVTLFRLVEQMDAGPILLQ
                     EEYFPDGTETAGELLDILAEKGSKLFLLGVKCIIEGTCSFIEQNSESATYAPKITNKE
                     AEVSWQSSAERIVNTVRALNPSPGAFLHTGSKRLKIWSARACSLSGAPGEVLGFDDGF
                     PVVGTAEGAVVLLAVQPEGKRKLDGSEWARGLRLKKGDFLH"
     gene            1719..>2086
                     /locus_tag="P5219_06985"
     CDS             1719..>2086
                     /locus_tag="P5219_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26538.1"
                     /translation="MTEPDISVLSDLISRYPSQNSPAPGWPKVVAVTGALGSGKTEWV
                     LNLALGFSGLGEKVTIADIDIINPYFCIRQVSEKLERRGFRIITTPENARWSDMSVVS
                     PVVTRALAEDDGRLLLDIGG"
BASE COUNT          435 a          600 c          620 g          431 t
ORIGIN      
        1 ccggagaatg cgcctcagca tcagcgccct ggaggaaccc tcccagcgca ggcccaggga
       61 tgatgaccgg aaaaaaggca ggcaggaccg ccgggagcac gacaaccagc cgaagcctgt
      121 aatgaacgac ggagaggcca gcgtcaccat cggagagctg ctcaggaact ccatgcagga
      181 agagtaaaac cggtggaagg ggtcgcccga aaaggcgccc ccttctttct tttcaaaata
      241 caatgaatga aagtgcggcg agatgatgga ttctctgtat cttcgggtat accccgaccc
      301 cgttctcagg aaagagacac aatccgtcac cgatttcggc gacggattca aggatcttgt
      361 ggcggacctc gttcggctca tgcaccagca tgacggcgtg gggttcgccg ctccccaggc
      421 cggcgtgtcc ctcaagctcg ccgtggtgtt ttaccagggt gagcttcacg tgctggcaaa
      481 ccccgtcctt gtcagctccg caggggagca ggacggagag gaagggtgcc tcagctttcc
      541 tgggattttc ggaaacgtga agcgacctct gaaagcggtg gtgcgccact tcgacatcaa
      601 cggcgcggaa aaggtgactg aggcggaggg ctttctggcc agggccttcc ttcacgagat
      661 ggaccacctt gagggaaggc tgctcatcga ccgcttttct cccctgaagc gaagcatggc
      721 aaaaaagaag ctcctccgcg cggaggcgga gcgggaggcg gagaagggat gatttccctt
      781 tcttccgggg aaacccgggc tgccctgtgg ttcttcggcg caggacggtt tgctgcccgc
      841 tgtctttccg tgctttcccg ggagatttcc tttgacctcg tggtgacgtc ccctccctcc
      901 gttggcggca ggggtctgaa agagctgccc tctcccgtgg aggaagcctg cctggaactg
      961 ggcataaccc ctcaccgctc cggaagcgca gggaaagacg aagttcttct ctcggccctc
     1021 gtagagacac cccccctggc gatcctggtg gtggatttcg ggcagaagat tcccgagcct
     1081 ttcctctccg aacccgcggg aggatgcctc aacattcacc cgtcgctgct gcccctctac
     1141 cgtggagccg cacctgtgca gcggtccctg ctgaacggag actccgtgac cggcgtaacg
     1201 ctcttccgcc ttgtcgagca gatggatgcg ggtcccatcc tccttcagga ggagtatttt
     1261 cctgacggta cagaaactgc cggtgaactt ttggacatcc ttgcagaaaa aggtagcaaa
     1321 ctttttctgt tgggtgtaaa atgcatcatt gaagggactt gttcgtttat agagcaaaat
     1381 tccgaatcag caacctatgc gccgaaaatt accaacaaag aggctgaagt ctcatggcag
     1441 tcatctgccg agcggatcgt caacacggtg agggctctca acccgtcgcc gggcgcgttt
     1501 cttcacacag gatccaaacg gctgaagatc tggagcgcga gggcctgttc cctttcgggt
     1561 gctcccgggg aagtcctcgg ttttgacgac ggttttccgg tggtgggtac tgccgaagga
     1621 gcggtggttc tcctggccgt ccagccggag ggaaaacgga agctcgacgg ctcggaatgg
     1681 gccagggggc tgcgcctgaa gaaaggagat tttcttcatt gaccgaaccc gacattagcg
     1741 tcctgtccga tcttatatcc cgatacccgt cacagaattc ccccgctccc ggatggccga
     1801 aagttgttgc cgtcacagga gctctcggtt ccggaaaaac ggaatgggtc ctcaatctcg
     1861 ccctcggttt ttccggcctt ggagaaaaag tcaccatagc cgacatcgac atcatcaatc
     1921 cctatttctg cattcgccag gtatctgaaa agcttgaacg aagaggtttc cggatcatca
     1981 ccaccccgga gaacgcccgg tggtcagata tgtcggtggt atctccggtg gttacccgcg
     2041 cccttgcgga ggacgacggc aggcttttgc tggatatcgg cggaga
//
LOCUS       DAOTHD010000376         2079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_63613_length_2079_cov_2.412055, whole genome shotgun sequence.
ACCESSION   DAOTHD010000376 DAOTHD010000000
VERSION     DAOTHD010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63613_length_2079_cov_2.412055"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..1830
                     /locus_tag="P5219_06990"
     CDS             115..1830
                     /locus_tag="P5219_06990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRX26539.1"
                     /translation="MEKQFLTLEKLQNRIISLEEERIAVTNALDIALKINDIQPDAAA
                     ETTVESILALCYERTGKLIGLRGGAFYLFREEDAGFQLAYASSPESKKKIEKELPALI
                     DDGTLAWVVQTERAVTAKLSTGEDAFLHALATPGRTMGAFIGILRDRKEDILDICYAL
                     LSIYFSQVAGVLQNRELFRVAKTENRSLSSAVNVLRETEEKLVSLNRTLEEMVRERTR
                     ELEKINERLLAEIAERKRKEEALLESEELSASLLNHSPNPILVVNPDTSVKYINFSLE
                     QITGYSLVEVINRKAPYPWWSEDDLHFMSEYYKQVTASGTSKREHLFRKKNGDPLWVE
                     VSMTTILGENGPKYSIANWVDITERKMAESAREHAEQLLTETNVKLRDTVDGIVVSMA
                     KVVETRDPYTAGHQERVARLAIAIAREMELPEEQVRGVGVAAVLHDLGKIDVPAEILS
                     KPSRLKDKEYSLIQDHCNAGYQILKNVDFPWPVAQAVLQHHERMDGSGYPSGISGDDI
                     LLESRILAVADVVEAMSSHRPYRPSLGLEQGLGEITRFRGKGFDETVVDACLAIFARG
                     ESPWE"
BASE COUNT          462 a          645 c          625 g          347 t
ORIGIN      
        1 ccgacagcgt ctggaaagtg gtggatcttc cctgttccgt catcgacatg acggcacggc
       61 aggctcacag gcagattacc gaaatttaca gccttttcaa aggcggcgat gacgatggaa
      121 aaacagttcc tcactcttga aaagctgcag aaccggatca tttcgctgga ggaagagcgc
      181 attgccgtca ccaacgccct cgacatcgcg ctcaagatca acgacatcca gccggacgcc
      241 gccgccgaaa ccacggtgga atccattctc gccctctgct acgaacgcac gggcaagctc
      301 atcggcctca gggggggagc tttctacctt ttccgggagg aggacgcggg gttccagctc
      361 gcctatgcca gctcccccga gtcaaaaaag aagatcgaaa aggaactgcc cgcactcatc
      421 gacgacggaa ctctcgcgtg ggtcgtccag acggagcggg cagtaacggc gaagctctcc
      481 acgggagagg acgccttcct ccacgccctg gccacgccgg gaaggaccat gggggccttc
      541 atcggcatcc tgagggacag gaaagaagac atcctcgaca tctgctacgc cctgctctcc
      601 atctatttca gccaggtggc gggagtcctg cagaaccggg agcttttccg ggtggccaaa
      661 acggagaaca ggagcctgtc ctcggccgtc aacgtcctgc gggagacgga ggaaaaactc
      721 gtctccctga accggaccct ggaggagatg gtccgggagc ggacccggga actggagaag
      781 atcaacgaac ggctcctggc ggagatcgcc gagaggaagc ggaaggaaga ggccctgctc
      841 gaaagcgagg agctttccgc gagcctgctc aaccactccc cgaaccccat actggtggtc
      901 aacccggaca cgtcggtgaa gtacatcaac ttctctctcg aacagatcac ggggtattcc
      961 ctggttgagg tcatcaaccg caaggccccc tatccgtggt ggagcgagga cgatctccat
     1021 ttcatgtcgg agtactacaa acaagtcacc gccagcggca cctccaaacg agagcatctc
     1081 ttcaggaaaa agaacggcga tcctctctgg gtcgaagtga gcatgaccac catcctcgga
     1141 gagaacggcc ccaagtactc catcgcaaac tgggtggaca tcaccgagcg gaaaatggcc
     1201 gagtcggcca gggaacacgc cgaacagctc ctcacggaga cgaacgtcaa gctcagggat
     1261 accgtggacg gcatcgtggt ctccatggcc aaggtcgtcg agaccaggga cccctacacc
     1321 gccggccacc aggagcgggt cgcccggctc gccatagcca tcgcccggga aatggagctg
     1381 ccggaggagc aggtcagggg ggtgggcgtg gcggcggtac tccatgacct cggaaaaatc
     1441 gacgtcccgg cggagatcct gagcaaaccc tcccggctga aggacaagga gtacagcctc
     1501 atccaggacc actgcaacgc agggtatcaa atcctgaaga acgtggactt cccctggccc
     1561 gttgcccagg ctgtcctcca gcatcatgag cggatggacg gctcagggta tccctccgga
     1621 atctccgggg acgacatcct cctggaatca aggattctcg ccgtggccga cgtggtggag
     1681 gccatgtcat cccaccgccc ctacaggccg agcctcggcc ttgagcaggg gctgggggaa
     1741 atcaccaggt tccgcgggaa gggattcgac gagactgttg tggatgcctg tctcgccatc
     1801 ttcgccaggg gagagtcccc ctgggagtga gaataacgca ctgtaggacg gccggaggct
     1861 tcagcctccg gccgtcctgt tttttttaca ggagcagccc cttctcagaa gaagcgccgt
     1921 ctcccctttt tccctttcac gccgtacatc tcctcgtccg cccgggcgat gagttccggt
     1981 gccgtggagc cgtcgtcggg gaagagggcg atacccatgc tgccccggac ggccaggagc
     2041 ctccctccgg cggaaaaggg ttccgaaaag gcgtttttc
//
LOCUS       DAOTHD010000377         2078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_63643_length_2078_cov_3.084528, whole genome shotgun sequence.
ACCESSION   DAOTHD010000377 DAOTHD010000000
VERSION     DAOTHD010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63643_length_2078_cov_3.084528"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /gene="nuoE"
                     /locus_tag="P5219_06995"
     CDS             complement(<1..440)
                     /gene="nuoE"
                     /locus_tag="P5219_06995"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRX26540.1"
                     /translation="MAFSADEIREKTRKIVEPWRNGKGGLIPILQAVQEQMGYLPPEA
                     LETISREMKIPSSEIYGVATFYAQFHLKPRGRHVVRVCRGTACHVRGSLKILDSVKEI
                     TGVEENQTTEDLRFTIEPVACLGACGLAPVMMIDTQTFGRLTPD"
     gene            649..1668
                     /locus_tag="P5219_07000"
     CDS             649..1668
                     /locus_tag="P5219_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HRX26541.1"
                     /translation="MKISYVHVEDALGMALSHDMTQIDVKNGFKGARFKKGQILREED
                     IPVLKSMGKETISILELGQGDVHEDDAASRLAERLSGEGITSEAPEEGKVSLSALWNG
                     LLVYDEESIHSINEDPDWIVATVADKVPVKKDERVAGIRIVPLIMREEQVRRGEEAAL
                     PMTVFPFAPLKTALVTTGKELAERRIRDGFAPKLQSKLAGYGASLMGQTVVGDEKEDI
                     AAAIRAFLEQGAELVITTGGMSVDPDDRTAGAIASVADDVRFKGVPTVPGAHLMLALS
                     GEAKIVGAPACVVHDEWTSLDPLLNRLFAGLIPTAAEVRRWGVGGMCRKCRECNYPFC
                     VFAAR"
     gene            1665..>2078
                     /locus_tag="P5219_07005"
     CDS             1665..>2078
                     /locus_tag="P5219_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRX26542.1"
                     /translation="MKNPEDLLRERGIRPTELRKEVLRILFGAGRPLSWKDTFTRFTE
                     KRVNKVSLYRTLSLLEEKGLVHKILGTDGAWHYCAHLVEGKKCPGNHPHFLCLACGRM
                     FCLEDQPLPTVQVPEGCVVEGKQLLAYGRCPSCSGT"
BASE COUNT          430 a          614 c          636 g          398 t
ORIGIN      
        1 atgtcgggag tcaggcggcc gaacgtctgg gtgtcgatca tcatcaccgg cgcgagaccg
       61 catgcgccga gacaggccac cggctcaatg gtgaaccgca ggtcctcggt ggtctggttc
      121 tcctccactc ccgtgatctc cttcacgctg tccaggattt tcaggctgcc ccggacgtgg
      181 caggccgttc cccggcatac ccggaccaca tggcgtcccc tgggcttgag atggaactgg
      241 gcgtaaaacg tggccacccc gtagatctcc gaggaaggta ttttcatttc cctggagatg
      301 gtctccaggg cctccggcgg aagatacccc atctgctcct gaacggcctg cagaatggga
      361 atgagacctc ccttgccgtt ccgccagggc tcgacgattt tcctggtctt ttcgcggatc
      421 tcatcagcgc tgaaagccat gattcccctc cttttttcct ctgcttcccc ttttcatcat
      481 cattgtgcac aaagtcacaa ttaaagttat catagcacaa cggacccacc cgttcaacaa
      541 acaaacagaa atgtataccc catacattct gcatccatgc tataatttca ccgcaaccgt
      601 gttgcattca ctcagcgcat catgaaggcg ggggactctt tttcaatggt gaagatcagc
      661 tacgttcacg tggaagacgc gctcggcatg gccctttccc acgacatgac ccagattgac
      721 gtgaaaaacg ggttcaaggg cgcccggttc aagaaagggc agattctccg ggaggaggac
      781 attccggtgc tgaagtccat ggggaaggag accatctcca ttctggagct gggccagggg
      841 gatgtccacg aggacgacgc ggcatcgagg ctggccgaac ggctcagcgg cgaggggatc
      901 acgtcggaag cgccggagga ggggaaggtg tccctttctg ccctctggaa cggcctgctg
      961 gtctatgacg aggaaagcat ccactccatc aacgaagacc ccgactggat cgtcgccacc
     1021 gtggcggaca aggtccctgt gaaaaaagac gaacgggtgg cggggatcag gatcgtcccc
     1081 ctcatcatga gggaggagca ggtccgcagg ggagaagagg ctgctctgcc tatgaccgtg
     1141 tttcctttcg cccctctcaa gacggccctg gtgaccaccg gaaaggaact ggcggaaaga
     1201 aggatccggg acggcttcgc cccgaagctc cagagcaagc ttgcggggta cggtgcgtcc
     1261 cttatggggc agaccgtggt gggtgacgaa aaagaagaca ttgccgcagc gatccgcgct
     1321 tttctcgaac agggtgcgga actggtgatc accaccggcg gcatgagcgt tgaccccgac
     1381 gaccggactg cgggagccat agcgtcggtg gccgacgacg tcaggttcaa aggagtcccc
     1441 acggttccgg gagctcacct catgctcgcc ctcagcggcg aggcgaagat cgtgggcgcc
     1501 ccggcctgcg tggtccatga cgaatggacg agcctcgacc ccctgctgaa ccgcctcttt
     1561 gccggcctca tcccgacagc ggcggaagtt cggcggtggg gcgtaggcgg aatgtgcaga
     1621 aaatgcaggg agtgcaacta tcccttctgc gttttcgccg ccagatgaaa aatccggaag
     1681 acctcctcag ggaacgggga atacggccca cggaactccg gaaagaggtg ctgcgcattc
     1741 ttttcggggc cgggaggccc ctttcctgga aagacacgtt cacccgcttc acggagaagc
     1801 gggtgaacaa ggtgtcgttg tacagaaccc tgtcgctgct cgaggagaag ggactggtcc
     1861 ataagatcct gggtaccgac ggggcgtggc actattgcgc ccatctcgtt gaagggaaaa
     1921 agtgcccggg gaaccatccc cattttctct gcctggcctg cggcaggatg ttctgcctcg
     1981 aagaccagcc gctccccacg gtgcaggttc cggaagggtg cgtcgtggag gggaagcagc
     2041 ttcttgccta cgggcgctgc ccctcctgct cgggaacg
//
LOCUS       DAOTHD010000378         2077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_63675_length_2077_cov_3.992087, whole genome shotgun sequence.
ACCESSION   DAOTHD010000378 DAOTHD010000000
VERSION     DAOTHD010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63675_length_2077_cov_3.992087"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..622)
                     /locus_tag="P5219_07010"
     CDS             complement(2..622)
                     /locus_tag="P5219_07010"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRX26543.1"
                     /translation="MESTIYLVRHGRPALPDYEMRFLGRTDLPLSPEGEEQAMALNQA
                     FAGIHLDRVFHSGLKRASETARIVAGDRNLPFAIVPELQEIAFGEWEVRSMKEIMASD
                     PEAFEARGQDFAGFRPPGGENFLDVQKRVWPAFTNLLDRWEGNLLITAHAGVFKTIIF
                     TLLDIPWQKLFSLRQDYCGVHVLTRFDGHLTVRQLNWMPQMGGPTP"
     gene            723..1712
                     /locus_tag="P5219_07015"
     CDS             723..1712
                     /locus_tag="P5219_07015"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HRX26544.1"
                     /translation="MKKSLVVTCSAPAAVRAAVEEGVAGNADIFYLEDAAPEKRAEVL
                     SSAHGLLSFFFNKEIQEEEYPLLENLELFQAISTGLDYLPFDKMPEKPVLACNAGGWA
                     PQIAEHTMALLLALARRIVPLHNDLMKGVFNRNGYSPMSLRGKILTVAGYGGIGRMTA
                     DLARGFGMKIRALNTSGKTDGDVDFMGTLKDLETVLPDTDVLLISLPLNRHTRGVIGK
                     KELSLMKKNAILLNVARAPLVVEEDLYLHLKENPDFQAGLDVWWKEPTWGGGDFALDF
                     PFLELPNVAGSPHNSNRCEGDMEMAALAAAENAAAFFKGEKFGGLIRREDYME"
     gene            1726..>2077
                     /locus_tag="P5219_07020"
     CDS             1726..>2077
                     /locus_tag="P5219_07020"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRX26545.1"
                     /translation="MARFTIATFNVNSVRSRMPVLQKWLSDHPVDALCLQETKVVDND
                     FPEADFKALGFRSLYRGEKSYNGVAILTKAPLDGFAVGFGDGEEPEGETRLLRARIGD
                     FCMVNTYIPQGKAID"
BASE COUNT          438 a          624 c          607 g          408 t
ORIGIN      
        1 cttacggtgt tggtccgccc atttgaggca tccagttgag ctgcctcact gtcagatgtc
       61 cgtcgaaccg ggtgaggacg tgcaccccgc agtagtcctg ccgcagggaa aagagcttct
      121 gccagggaat gtccagcaga gtgaagatga ttgttttgaa gacccctgcg tgggcggtga
      181 tgaggagatt gccctcccac cggtccaaaa ggttggtgaa ggccggccag acccgctttt
      241 ggacgtcaag gaagttttct cctcccggcg ggcggaaacc ggcgaaatcc tgtcccctgg
      301 cctcgaaggc ttcggggtcc gacgccatga tttccttcat ggacctgacc tcccactcgc
      361 cgaaggcgat ctcctggagt tccgggacga tggcgaaggg cagattccga tcccctgcga
      421 cgattcgggc tgtctctgac gcccgcttca ggccgctgtg gaagaccctg tcgaggtgga
      481 ttcccgcgaa ggcctgattc agggccatgg cctgctcttc tccttcggga gagagaggca
      541 ggtccgtcct tccgagaaag cgcatttcgt agtccggcag cgccggccgc ccgtggcgga
      601 ccaggtagat ggttgattcc atgatgctcc tccttccgga gaatgtattc cctgtttgta
      661 cggaacgttt catagggtac aatacaatgc agggaaatac cagcgaaagg agaactgccg
      721 acatgaaaaa atcactcgtg gtgacctgct ccgctcccgc agccgtccgg gctgccgtgg
      781 aggagggggt tgccggcaac gccgacattt tctaccttga ggacgccgcc ccggaaaaaa
      841 gggcggaggt cctttcctcc gcccacgggc ttctgtcctt cttcttcaac aaagaaatac
      901 aggaggaaga atatcctctt ctggaaaacc tggaactctt ccaggccatt tccacgggcc
      961 tcgactacct tcccttcgac aagatgcccg aaaaacccgt tcttgcctgc aacgccggag
     1021 gatgggcccc ccagatcgcc gaacacacca tggcccttct gctcgcgctt gcgagaagga
     1081 tcgttcctct tcacaacgac ctcatgaagg gtgtcttcaa ccgaaacggc tattctccca
     1141 tgagccttcg gggaaaaata ctcaccgtgg cgggctacgg cggcatcggg cgcatgaccg
     1201 cagaccttgc ccgggggttc ggcatgaaaa tcagggcgct gaacaccagc ggaaagaccg
     1261 acggcgacgt ggacttcatg ggaaccctga aggaccttga aacggtcctc cccgatactg
     1321 acgtgctgct tatctccctg cccctgaaca ggcacaccag gggcgtcata gggaagaagg
     1381 agctctccct catgaaaaag aacgccatac tgctcaacgt ggcccgggct cccctggttg
     1441 tggaggagga tctctacctc cacctgaagg agaaccccga cttccaggca ggcctggacg
     1501 tgtggtggaa ggaacccacc tggggaggag gagattttgc cctcgacttc cccttcctcg
     1561 agcttcccaa cgtagccggt tcgccccaca attcgaaccg gtgcgaaggg gacatggaaa
     1621 tggcagccct ggccgctgct gaaaacgcgg ccgctttttt caaaggagag aaattcggcg
     1681 ggctcatccg ccgcgaagac tatatggaat gagagggaga ctgtcatggc tcgcttcacc
     1741 atagcaacgt tcaacgtaaa ttctgtccgc agccggatgc ccgttctgca gaagtggctt
     1801 tccgatcatc ccgtggatgc cctgtgcctc caggagacca aggtggtgga caacgacttc
     1861 cccgaggccg acttcaaggc tctggggttt cgcagcctct acaggggaga gaaatcctac
     1921 aacggtgtgg ccatcctcac gaaggcaccc cttgacggat ttgccgtcgg tttcggagac
     1981 ggcgaggagc ctgaggggga aacccgcctt ctgcgggccc ggatcgggga cttctgcatg
     2041 gtgaacacct acatccccca gggaaaggcc atcgacc
//
LOCUS       DAOTHD010000379         2077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_63689_length_2077_cov_2.764590, whole genome shotgun sequence.
ACCESSION   DAOTHD010000379 DAOTHD010000000
VERSION     DAOTHD010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63689_length_2077_cov_2.764590"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="P5219_07025"
     CDS             complement(<1..256)
                     /locus_tag="P5219_07025"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HRX26546.1"
                     /translation="MAPGTPGRRLFFAGLLLLLGGAALWRLLAGDMPLSPAAVADILF
                     SPSPDASPQEILVVRSVRLPRLLASLGAGASLAVSGAVLQG"
     gene            complement(262..1152)
                     /locus_tag="P5219_07030"
     CDS             complement(262..1152)
                     /locus_tag="P5219_07030"
                     /inference="COORDINATES: protein motif:HMM:NF017944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016852 - sirohydrochlorin
                     cobaltochelatase activity [Evidence IEA];
                     GO_process: GO:0019251 - anaerobic cobalamin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin cobaltochelatase"
                     /protein_id="HRX26547.1"
                     /translation="MRKNMVTVLLLGLAAVGLLAGAAAASGKAPAEKQGILLVAFGTS
                     EPEARGAIDRIVETARKTFPDAEVRLSYTSNIIRRKILKEEGLAIDSPLIALAKMQDE
                     GFTSVTVQSLHIIAGEEFHQLASVVRTLESVRGKYGFSRLSLGTPLLTTLEDYRKTAE
                     LLKRKYGSLGGDGGVVVFMGHGTHHGGNASYAQMQMIFDEEGLPFILGLVEGYPDLEG
                     VKRRLASLKPRKVTLVPFMVVAGDHARNDMADEEDPESWISVLRKEGYDVEAVLKGLG
                     DGEGLAELFADHIREASGSR"
     regulatory      complement(1323..1487)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1546..>2077)
                     /locus_tag="P5219_07035"
     CDS             complement(1546..>2077)
                     /locus_tag="P5219_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRX26548.1"
                     /translation="LQTISSRFVAPTDPVVVTIGQINGGIARNIIAGSVTLEGTIRTV
                     KPETRDRVHRELEKMVPALAEAMGGSAVVEIQRGTPPLVCDDGLVDRLQAVTEKLLGK
                     DRYVSLPDPSMGGEDFAFIMEKVPGLFIRLGVKRPGGPVTPGHSVDFFADESAIAPGL
                     AVFAGTALDFLGVPLE"
BASE COUNT          360 a          716 c          635 g          366 t
ORIGIN      
        1 cgccctggag gaccgccccc gacaccgcca gggacgcccc cgcacccagg gaggccagca
       61 gccggggcag ccggactgat cggaccacaa ggatctcctg gggcgatgcg tccggggagg
      121 gcgaaaagag gatgtccgcc accgccgccg gggaaagggg catgtccccc gcgagaagac
      181 gccacagggc cgctcccccc agaaggagga gcagccccgc aaaaaacagc cttcgtcccg
      241 gtgttcccgg agccactgcc tctacctgct cccggaggcc tcccggatat ggtccgcgaa
      301 cagctccgcc aggccttctc cgtcgccgag acccttcaaa acggcctcaa cgtcgtagcc
      361 ttcctttcgg agcacggaga tccacgattc cgggtcctcc tcgtcggcca tgtcgttccg
      421 ggcgtggtcg ccggccacca ccatgaaggg gacgagggtc accttccggg gcttcaggga
      481 cgccaggcgc cgcttcactc cctccaggtc cgggtagccc tccacaagtc ccaaaatgaa
      541 gggaaggcct tcctcgtcaa agatcatctg catctgggca taggacgcgt tccccccgtg
      601 atgggtgccg tggcccatga acaccacgac tccgccgtcc ccgcccaggg agccgtattt
      661 cctcttcaga agctccgccg tcttccggta gtcctcaagg gtggtgagga gcggtgtgcc
      721 cagtgagagc ctggaaaatc cgtactttcc ccggactgac tccagggtgc ggaccaccga
      781 cgcaagctgg tggaattcct ctccggcgat gatatgaagg gactgcaccg tcaccgacgt
      841 gaagccctcg tcctgcatct tcgccagggc gatgaggggg gaatctattg cgagtccctc
      901 ctccttcagg atcttcctgc ggatgatatt ggacgtgtag gagagccgca cttccgcgtc
      961 cggaaacgtt ttccgggcag tctccacgat ccggtcgatg gcgccccggg cctcgggctc
     1021 ggaggtcccg aaggccacaa gcaggatccc ctgcttttct gcaggtgcct ttcccgacgc
     1081 ggcagccgca cccgcaagca gaccgacagc cgcaagtccc agcagcagta ccgtaaccat
     1141 gttcttcctc atcctgttct cctcccgaaa tgatgaaaga tgtccgggga atgcgccggg
     1201 aggaaaggag tccgcccccc gcgtccgaag acgaaagggg ccgtcctgtg cggatcggcc
     1261 cctttgaatg gatgcggttt cggcggtggt gctgcgaaaa ggcggcctcg attcctcgcg
     1321 agtcttccct ggttccgcac gaccggtctc ctggcttgcc ctcgtcctcc cccatccctt
     1381 cccggaggtt tttcctccag tggaccgatg ggttcgtcag gcttacagtg gcgggtccgc
     1441 gccggatttt caccggcttc ccgagcatcg tgcggcgaaa gcccttcggg cttccgtcct
     1501 gatattatga ccttccggcg gagtttgtca aggaagggag ccagcctact ccagcggcac
     1561 cccgaggaag tccagggccg ttccggcgaa gaccgcgagc cccggggcga tggccgactc
     1621 gtcggcgaag aagtccaccg agtgaccagg ggtcaccggc cctcccggtc gcttcacgcc
     1681 gagacggatg aagagccccg gcaccttctc catgatgaag gcgaaatcct ccccgcccat
     1741 ggacgggtcg ggaagggaca cgtaccggtc cttccccagc agcttttccg tcaccgcctg
     1801 cagcctgtcc acgagcccgt cgtcacagac caggggcggg gttccccgct ggatctccac
     1861 aaccgctgac ccgcccatgg cttcggcgag agccgggacc atcttttcaa gttccctgtg
     1921 gaccctgtcc cgggtctcgg gcttcacggt ccggatggtt ccctcgaggg tcacgcttcc
     1981 ggcgatgata ttccgggcaa tcccgccgtt aatctgcccg atggtcacca ccacgggatc
     2041 ggtgggggcc acgaaccggg aggagatggt ctggagg
//
LOCUS       DAOTHD010000380         2076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_63722_length_2076_cov_3.451757, whole genome shotgun sequence.
ACCESSION   DAOTHD010000380 DAOTHD010000000
VERSION     DAOTHD010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63722_length_2076_cov_3.451757"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="P5219_07040"
     CDS             complement(<1..510)
                     /locus_tag="P5219_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26549.1"
                     /translation="MKRNVTAAVFCAIFLLTFTLGALAFEGVPKNHKKLLKNEDYAGA
                     YEGMAAVMAEAKEKLDAEEYKALEQENAGAIAQSVREDMESGGSTEAEAYSTAYWMRS
                     EYVSRGVLVWDWLRKNPKGIQGYYRLKSSEADGYVTIMESDEKDVYCVYFCVTMKETP
                     DLAGELDGMG"
     gene            complement(588..881)
                     /locus_tag="P5219_07045"
     CDS             complement(588..881)
                     /locus_tag="P5219_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26550.1"
                     /translation="MKEHTGYFRSVKVLPGYRMEVETATCSRIEFDFTSRLNTIRFGI
                     LKDGGVFSTASTDGDYILFRRNDEIRVKISAPEFIDLLLVDRTATRAGGDSGN"
     gene            complement(1000..>2076)
                     /locus_tag="P5219_07050"
     CDS             complement(1000..>2076)
                     /locus_tag="P5219_07050"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRX26551.1"
                     /translation="SPRTVIFGLTPWTFGIPSVLSLYWSRTGGLDESLDLMDECMPLY
                     VKLAGGHGTGADVMMRAEAHLCRGEEKEAETLSHKALYIARGAGQTSLCLCAELVLGR
                     LAILRGDGAAYGAVRENISGYRKESSERSIARMVELCLAVLDLSVGKTEKIPVWLRKA
                     ESLRKVLYIQGHPYGLMFYARLLFLEKRYPELYGMAEPAMEMASGMKYELPRLWMLLH
                     LAASKNDEGQSVQALEYLGRALLTALPDRMHLPFAEFGISMVPLLQAVKKDFGGEALE
                     PVMNLCRRGAAGLSSVLARIEPKLSLLTPREREIALLARDRLTAAEIGSRLFISENTV
                     KSALKLIYSKLDVHSRSELVKKDF"
BASE COUNT          380 a          649 c          565 g          482 t
ORIGIN      
        1 tcccattccg tcgagttcgc ccgcaaggtc cggggtctct ttcatggtca cgcagaagta
       61 gacgcaatag acgtcctttt cgtcgctctc catgatggtc acatatccat cggcctcgct
      121 gctcttcagc cggtaatacc cctggattcc cttcggattt ttccggagcc agtcccacac
      181 caggactccc ctgctgacgt attcgctccg catccagtat gccgtggagt aagcctcggc
      241 ctcggtggag ccgccggact ccatgtcttc cctgacgctt tgggctatgg ccccggcgtt
      301 ctcctgttca agggctttgt attcttcggc gtcgagtttc tctttcgctt cggccatcac
      361 cgcggccatc ccttcgtaag ctccggcgta gtcttcgttt ttcaggagct tcttgtggtt
      421 tttcggaact ccctcgaaag cgagcgcccc caaagtgaac gtcagaagaa aaattgcaca
      481 gaacaccgcc gccgttacat ttcttttcat agcattccct cccctgtatt ttttcttctt
      541 cattcccggc cgcccgctcc gccggcagca tcttcccggg cttcacgtca gttcccggaa
      601 tcacccccgg cccgcgtggc ggttctgtcc acgagaagca ggtcgataaa ttcaggcgcc
      661 gatattttta ccctgatctc atcgtttcgc ctgaacagga tatagtcacc gtccgttgag
      721 gctgtggaga aaacaccgcc gtccttcagg attccaaaac ggattgtatt cagccgggac
      781 gtgaaatcaa actcgatgcg ggagcaggtg gccgtctcga cctccatcct gtagcccggc
      841 aggactttca cgcttctgaa gtagcctgtg tgttccttca ttccgtccgt cacttccttg
      901 aggaaaattg tccttccgtg gcctgtgccc ctattgtggg actggaatcc atgacccgca
      961 cccccatttt caggtgggtg cgggggtgat ttctccatcc taaaagtctt ttttgaccag
     1021 ttcgcttctg gagtgaacgt ccagcttgct gtatataagc ttgagggctg acttgaccgt
     1081 gttttccgag atgaagagcc gggagccgat ctcggctgcg gtgagccggt ccctcgcgag
     1141 aagggctatc tcccgttccc ggggagtcag aagggataat ttcggttcaa tccgggccaa
     1201 taccgaagag agccccgcag cgccgcgcct gcacaggttc atgacaggtt cgagcgcttc
     1261 tccaccgaag tccttcttca ccgcctgcag cagaggtacc atggagatgc cgaactcggc
     1321 gaagggaaga tgcatcctgt cgggaagggc cgtgaggagc gcccgcccca ggtactccag
     1381 ggcctgaaca ctctgcccct cgtcattttt cgaagccgcg agatgaagca gcatccagag
     1441 ccggggcagc tcgtacttca tcccggacgc catttccatg gcgggctccg ccattccgta
     1501 gagttccggg taccgctttt caaggaagag gagccttgca tagaacatga gtccataggg
     1561 atgtccctgg atatacagga ccttccggag gctttccgcc ttccggagcc ataccggtat
     1621 tttttccgtc tttccgacgg agaggtcgag gacggccagg cagagttcaa ccattcgggc
     1681 gatggacctt tcagaggact ctttccgata tccggagatg ttctccctga cggccccgta
     1741 ggcagccccg tctccccgaa gaatcgccag gcggcccagc accagctccg cgcagaggca
     1801 caggctggtc tggccggctc cccgggcaat atacagggct ttgtggctca gggtttccgc
     1861 ttccttttcc tcgccgcggc agaggtgggc ctcggccctc atcatgacat ccgctcccgt
     1921 cccatggccg ccggccagct tcacatacag gggcatgcat tcgtccatga ggtccagaga
     1981 ctcgtcgaga ccgccggtac ggctccagta gaggctcagc accgagggaa tgccgaaggt
     2041 ccagggcgtc aggccgaaaa tgacggtccg gggaga
//
LOCUS       DAOTHD010000381         2076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_63729_length_2076_cov_3.020287, whole genome shotgun sequence.
ACCESSION   DAOTHD010000381 DAOTHD010000000
VERSION     DAOTHD010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63729_length_2076_cov_3.020287"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="P5219_07055"
     CDS             complement(<1..1172)
                     /locus_tag="P5219_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRX26552.1"
                     /translation="MKKNIKVTLNGKTVYGYEGQRILDLCSDCGVEIPTLCYDPHLSL
                     HGGCSVCLVEVEGAKTLLRACANTITHGMVIRTETERAVSARRTALELLLSDHVGDCR
                     PPCTLTCPANGNVQAYINLAAQGKYQESLDVLHHHVTLPACIGRVCPAPCEKKCRRNF
                     VDDAPVSIREIKRFVGDWALRNDYLGVIPETEDNGKTVAVVGGGPAGISAAYYLRLKG
                     YRPVIFEKETLLGGMMRYGIPDYRLPQAVLQKEIDWLLAHGVEVRTGMTLGKNITLDE
                     LRRDFDGVILAMGCWKSSPMRVPGEDLDGVLGGINFLYDVKTNPDVKIGRRVAVVGGG
                     NTAMDACRSARRLGAEEVSVLYRRSREEMPADDLEIEEATGEGVNFIYLAAPKSIEG"
     gene            complement(1198..>2076)
                     /locus_tag="P5219_07060"
     CDS             complement(1198..>2076)
                     /locus_tag="P5219_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRX26553.1"
                     /translation="PFPANSGVFGKPTNINNVETIANIPWILLNGAAKFAEYGIGKSR
                     GTKVFALAGKIKKGGLAEVPMGMPLREVIFNVAGGIAENKKFKAVQLGGPSGGCIPES
                     LLDTPVDYESINATGAIMGSGGMVVMDETTCIVDVAKFFLAFVQKESCGKCPFCRIGT
                     KRMLEILERITEGKGVMEDLDTLLDLARQVKEGSLCGLGQTAPNPVLTTLKYFREEYE
                     AHIRDKKCPAKVCTSLIHYVIDPAACIGCTKCARNCPVSCIAGEVKKPHVIDDEKCIR
                     CGQCKKVCPVGAISVE"
BASE COUNT          329 a          631 c          677 g          439 t
ORIGIN      
        1 ccctcgattg atttcggggc cgcgaggtag atgaagttca cgccttcccc tgtggcctcc
       61 tcgatctcga gatcgtctgc gggcatttcc tcccggctcc tccggtagag caccgacact
      121 tcctcggcgc cgaggcggcg ggcagaacgg caggcgtcca tggcggtgtt gcctcctccc
      181 accacggcta cccttctgcc gattttcacg tcagggttgg tcttcacgtc gtagaggaag
      241 ttgatgcctc caaggacacc gtccaggtcc tccccgggca cccgcatggg tgaggacttc
      301 cagcagccca tggccagtat gacgccgtcg aaatccctgc ggagttcgtc gagggtgatg
      361 ttctttccca gggtcatgcc ggtgcgcacc tccacgccgt gggcgaggag ccagtcgatc
      421 tccttctgga ggacggcctg aggaagacgg tagtcgggaa tgccgtagcg catcatgccg
      481 ccgagaaggg tttccttctc gaagatgacg ggccggtagc ccttcaggcg caggtagtag
      541 gccgcggaga ttccggcggg gccgcctccg acgacagcga cggtcttgcc gttgtcttcg
      601 gtctccggga tgacgccgag atagtcgttc ctcaacgccc agtccccgac gaaccgcttg
      661 atctcccgga tggacacggg cgcgtcgtcc acgaagtttc tgcggcactt cttctcgcag
      721 ggggcggggc agacccggcc gatgcacgcg ggaagggtga cgtggtggtg gagcacgtca
      781 agggattcct ggtatttccc ctgggcggcc aggttgatgt aggcctggac gtttccgttg
      841 gcggggcagg tgagggtgca gggggggcgg cagtcgccca cgtggtcgga aagcagcagc
      901 tccagggcgg tccggcgggc gctcacggcc cgctccgtct cggtcctgat gaccataccg
      961 tgggtgatcg tgttggcgca cgcccggagc agtgttttgg ccccttccac ttccacgagg
     1021 cagacggaac agccgccgtg gaggctcagg tggggatcgt agcagagggt gggtatttcc
     1081 acgccgcagt cggagcagag atcgagaatt ctctgccctt cgtatccgta gactgttttt
     1141 ccgttgaggg tgacttttat gtttttcttc atggcagctc gaaatcctcc cgaaaggtta
     1201 ttccaccgag atggcgccca cggggcagac cttcttgcac tgtccgcagc ggatgcactt
     1261 ctcgtcgtcg atgacgtggg gtttcttcac ttctcccgcg atacaggaga cggggcagtt
     1321 ccgggcgcat ttcgtgcagc cgatgcaggc tgccgggtcg atgacgtaat ggatgaggct
     1381 ggtgcatacc ttggcggggc actttttgtc ccggatgtgg gcctcgtact cctcccggaa
     1441 atatttcagc gtggtcagaa ccgggttcgg cgcagtctgg cccaggccgc agagagaccc
     1501 ttccttcacc tggcgggcca ggtcaagcag ggtgtccagg tcttccatga cccctttgcc
     1561 ttccgtgatc cgctcgagga tctccagcat ccgcttggtg ccgatgcggc agaaggggca
     1621 cttgccgcag gattccttct ggacgaaggc caggaagaac ttggccacgt ccacgatgca
     1681 ggtggtttcg tccatgacca ccatgccgcc ggagcccata atggcgcccg tggcgttaat
     1741 ggattcatag tccaccggag tgtccagcag cgattcgggg atgcatcctc ccgaggggcc
     1801 gccgagctgg acggccttga actttttgtt ctcggcgatg ccgcccgcca cgttgaagat
     1861 gacctcccgc aggggcatcc ccatgggaac ctcggcgagg ccgcccttct tgatctttcc
     1921 cgccagggcg aagaccttgg tgccccggct cttgccgatg ccgtactccg cgaacttcgc
     1981 cgccccgttc agcagtatcc aggggatgtt ggcgatggtc tccacgttgt tgatgttcgt
     2041 cggtttgccg aagacgccgc tgttggccgg gaaggg
//
LOCUS       DAOTHD010000382         2075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_63764_length_2075_cov_3.501485, whole genome shotgun sequence.
ACCESSION   DAOTHD010000382 DAOTHD010000000
VERSION     DAOTHD010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63764_length_2075_cov_3.501485"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="P5219_07065"
     CDS             <1..1180
                     /locus_tag="P5219_07065"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HRX26554.1"
                     /translation="LRTGKAYIAGDLAMDPYYVSHSEEARSLVAVPISYKGKDWGVLC
                     LDSAVRYAFGVRERDLLGLVGFYVALHLEEVEARAELEKKAKQLGFLHRVIQHFAAER
                     TNEDLSRRIVDILGAELGFPLVGILAPGGEDGENMTLLAGHPGDTGGDDMMSRFASRA
                     RDALRTGLPVAGGENERPGTLAVPLSFNGTVFAVLAAWNERGFTPSEKELLEITAEHG
                     ATFWVLNNLLAERRHEALIDPLTQVWNRRYIMRRLEEENSRISRNGSRGAVVLVDLGD
                     FKSINDRFGHVAGDEVLRETASLMSKNLRTCDMIGRYGGDEFLLYLPDVTVDQAAAAM
                     LRMEDRVAGLKIPGVNAAVVLDYGIASCPGDGNDLVEAIGVADARMYENKARRKAETA
                     "
     gene            1177..1620
                     /locus_tag="P5219_07070"
     CDS             1177..1620
                     /locus_tag="P5219_07070"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRX26555.1"
                     /translation="MILSLHHPSFTVENLERSAAFYRDVLGLTLEGIWERDPAYSEGV
                     TGIPGAHLKVAYFTLPNASFELVEYMEGKGVKIDTSTNNTGSAHVCFVTDDFDGLMDR
                     LRRAGAVFSGEVNIVPGGSNRGKKIVYVEDPDNNTLEIISSTVSG"
     gene            1704..1898
                     /locus_tag="P5219_07075"
     CDS             1704..1898
                     /locus_tag="P5219_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26556.1"
                     /translation="MLGQYYLATGIGAFSLVAVAVITGLFGRRLRKVFSAPKVLLVHK
                     ASALAGAFLALLHVLGVHGY"
BASE COUNT          444 a          612 c          657 g          362 t
ORIGIN      
        1 cctgaggacg ggcaaggcct acatcgccgg cgacctggcc atggacccct actacgtttc
       61 ccactctgag gaagcccggt ccctggtcgc cgttcccatc tcctacaagg gaaaggattg
      121 gggggtgctc tgcctggaca gcgccgtgag gtacgccttc ggtgtccggg aaagggacct
      181 cctcggtctc gtaggcttct atgtggccct ccatctcgag gaggtggagg ccagggccga
      241 acttgaaaaa aaggcgaagc agctcgggtt ccttcacagg gtgatacagc actttgccgc
      301 ggaacggacg aacgaggatc tgtccaggag aattgtggac atcctggggg cggaactcgg
      361 cttccccctg gtgggaatcc ttgctcccgg aggggaggac ggagaaaaca tgaccctgct
      421 ggccggccat cccggcgata cgggaggaga cgacatgatg tcccgtttcg cctcccgggc
      481 gagggacgct ctgcggacag gactccccgt cgcaggggga gaaaacgaac ggcccgggac
      541 cctggcggtg cccctctcgt tcaacggaac ggtgttcgcc gtccttgccg cctggaacga
      601 gagaggcttt accccctccg aaaaggagct tctggaaatc acggcggaac acggcgccac
      661 cttctgggtg ctgaacaacc tgttggccga acggcgccac gaggccctga tcgaccccct
      721 cacccaggtg tggaaccgca ggtacatcat gcggcggctg gaagaggaaa attcgaggat
      781 ctccaggaac ggaagcaggg gagcggtggt cctggttgac ctcggggact tcaagagcat
      841 caacgaccgg ttcggccacg tggcgggcga cgaagtgctc agggaaacag cgtccctcat
      901 gagcaaaaac ctgagaacct gcgatatgat aggacgatac ggcggagacg aattccttct
      961 ttatctcccg gacgtaaccg tcgaccaggc cgccgcggcc atgctccgca tggaagaccg
     1021 ggttgccggc ctgaaaatcc ccggagtgaa cgccgcagtg gtgctggatt acggcatagc
     1081 ctcctgcccg ggtgacggaa acgacctcgt ggaggccata ggagtcgccg acgcaaggat
     1141 gtacgagaac aaggcgagac gaaaggcgga aactgcatga tcctgtcgct gcatcatccg
     1201 agctttacag tggagaacct cgagcggtcc gcggcgttct acagggatgt cctcggcctc
     1261 accctcgaag gaatatggga gcgtgacccg gcctattccg agggggtcac cggcattccc
     1321 ggggctcatc tgaaagtggc ctatttcacc ctgcccaacg cctccttcga gctggtggag
     1381 tacatggaag gaaagggagt caagatcgac acgtccacca acaacacggg cagcgcccac
     1441 gtctgtttcg tcaccgatga tttcgatggc ctgatggacc ggctgcgccg ggcgggagcg
     1501 gtcttctccg gggaggtcaa catcgttccc ggcggatcca acaggggaaa gaaaatcgtc
     1561 tacgtggagg accctgacaa caataccctg gagattatct cgtccacggt ttccggctag
     1621 cacgggcagg ggaggagggc tctttctccc ggccccgcag gagtgcggca actccggcat
     1681 gacataagca aggagggaag aacatgctcg gacaatatta tctggccaca ggcatcgggg
     1741 ctttttcgct cgtggccgtc gcggtaatta ccggattgtt cggacggcgg ctcaggaagg
     1801 tgttttcagc gccgaaagtg cttcttgtcc acaaggcatc agcccttgcc ggcgccttcc
     1861 tggcactgct ccacgtactg ggcgttcacg gatactgaga aaaccggccc gccgccggct
     1921 gcaatgtaaa aagaaagggg ggacccccgt gccgccgctg acgcaccaga aaatactcgt
     1981 cgcggaggat tccctctccc agagggcaat aatctccaga cacctgagaa aatggaatta
     2041 cgatgtgctg gaggcttccg acggcctcca ggccc
//
LOCUS       DAOTHD010000383         2073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_63856_length_2073_cov_2.987116, whole genome shotgun sequence.
ACCESSION   DAOTHD010000383 DAOTHD010000000
VERSION     DAOTHD010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63856_length_2073_cov_2.987116"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="P5219_07080"
     CDS             complement(<1..333)
                     /locus_tag="P5219_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26557.1"
                     /translation="MMKNATDYPRMLKILASVLEKLTDEEIRGLLAGEMNLVPETKAK
                     KQAAKWPSPAADEPDITAVEELLSACGSREEGRKILSSLKLKKASLLKIAEGQGISVR
                     KGDTVAVIS"
     gene            complement(330..1670)
                     /locus_tag="P5219_07085"
     CDS             complement(330..1670)
                     /locus_tag="P5219_07085"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRX26558.1"
                     /translation="MTQSPFSFLRKIAAVLFGSGTPELTEQCLSEEPEKEVRKETPEP
                     SPESGPETAGETDKEEDEIITGEVPPEYADETILQLNDEPERGVTGETIPASFPEPWD
                     EAAAEHADQEEDIPAEAVIVIPAAEPSGEESIVPPFREGTKTRILSVMNYKGGVGKTT
                     VTANLAAALAKRGKRVLAIDLDPQSSLSFSFFHVDEWKQKFAETKTIKNWYDAFIDKD
                     FNWNLERLVVDPKLQIRGSGKLHLICSHLALINIEIELSSKLSGSTERELRNNFLRVH
                     SRLAQGLKSLDGRYDVVLIDCPPSFNIVTKTAVAASDYLLVPTIPDYLSTLGIDYLNN
                     KVESLVETYNRYVDICEDHEFNHISPVVLGVLFTMIQLSAGIPIRAQQPFIRDVRKRE
                     YPVFETMLRENRTMYSNISATPLPVILRRGAGQTQQNVISELEDLASEVLERMK"
     gene            complement(1824..>2073)
                     /locus_tag="P5219_07090"
     CDS             complement(1824..>2073)
                     /locus_tag="P5219_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26559.1"
                     /translation="VKFGGVWRFIQREADGSEDFSYMMKRVQDQGGQASMLRILTETA
                     AVGHNRRFDFDDRDALPKAVKALSAVTCRLLSGDRASQ"
BASE COUNT          372 a          583 c          597 g          521 t
ORIGIN      
        1 gctgatcacg gcgacggtgt ctcccttccg gacgcttatt ccctggcctt cggcgatctt
       61 cagcagcgag gctttcttca gcttcaggga ggaaaggatt ttccggcctt cttcacggct
      121 gccgcaggcg gagagcagtt cctccaccgc cgtgatgtcc ggttcgtcag cggcagggga
      181 tggccatttc gccgcctgtt tcttcgcctt cgtctcgggg acaagattca tttcccccgc
      241 aagaagcccc cggatttcct cgtctgtaag cttttccagc accgaggcca gtatcttcag
      301 catccttgga tagtctgtgg catttttcat catttcatcc tctccagtac ttcggacgca
      361 aggtcctcaa gttcgctgat gacattctgc tgggtctgcc ccgccccgcg gcggagtatg
      421 acgggcagag gcgtggcgga aatattggaa tacattgtcc ggttttccct cagcatggtt
      481 tcaaagacgg gatattccct cttgcggaca tccctgataa agggctgctg tgcccttatg
      541 gggattccgg cggacagctg gatcatagtg aagagcactc ccagcacgac ggggctgata
      601 tggttgaatt cgtggtcttc acagatgtcg acgtaccggt tgtaggtttc caccagggat
      661 tcgaccttgt tgttcaggta gtctatgccc agggtggaga ggtaatcggg gatggtgggc
      721 accaggaggt aatcgctggc cgccacggcg gtttttgtca caatattgaa actgggaggg
      781 cagtcgatga gaaccacgtc gtaccgtccg tcgagtgact tgagcccctg ggcgagcctg
      841 gaatggaccc ggaggaaatt gttcctcagc tcccgttcgg tagagccgct cagcttgctg
      901 ctaagttcta tctcgatgtt gatgagggcg aggtgggaac agatgagatg gagttttccg
      961 ctcccccgga tctggagttt cgggtcgacg accagccttt cgagattcca gttaaaatcc
     1021 ttgtcgatga aggcgtcgta ccagttcttg atggtcttcg tctcggcaaa tttctgtttc
     1081 cactcgtcca catggaagaa agagaaggac aggctggact ggggatcaag atcgatggcc
     1141 aggacccttt tccctctctt tgccagtgcc gcggccagat ttgccgttac ggtagttttg
     1201 ccgacaccgc ccttgtagtt catcacggaa agaatgcggg tttttgtccc ttcccggaac
     1261 ggagggacga ttgattcttc gccggaaggt tctgcggcag gaatgacgat gacagcttcg
     1321 gcggggatgt cctcttcctg atccgcatgt tcggccgccg cttcatccca gggctccgga
     1381 aaagacgcag ggatggtttc ccctgtcact ccccgttccg gttcgtcgtt caattgcagg
     1441 attgtttcat ccgcgtattc cggcggcact tcaccggtga ttatctcgtc ctcctcttta
     1501 tccgtttctc cggctgtttc cggaccggac tccggtgaag gttcaggagt ctctttccgg
     1561 acttcttttt ccggttcctc ggaaaggcac tgttccgtta attccggagt tccggaaccg
     1621 aagagaacgg cagctatttt tcgcaagaaa ctgaagggtg attgggtcac gacgcaaaca
     1681 tctccctttt ttcggatcgt ggtgatatgc cagcccgcag ccgccccctt gagcgacgcc
     1741 tccaggaacg ggcttccttt ccgcgcgcag gacgcgagag cggctcctgc cggaaatccg
     1801 gggaataccc tgtgcttctg cgtttactgt gatgcccggt ctcccgaaag aagcctgcag
     1861 gtgacggcgc tgagggcttt gactgccttg ggcagggcgt ccctgtcgtc gaagtcgaac
     1921 ctcctgttgt gcccgacagc cgccgtttcg gtaaggatcc ggagcattga agcctgcccg
     1981 ccctggtcct gcacacgctt catcatgtag gaaaagtctt cgctgccgtc tgcctcgcgc
     2041 tggatgaagc gccagacgcc gccgaacttg aca
//
LOCUS       DAOTHD010000384         2069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_64041_length_2069_cov_2.382820, whole genome shotgun sequence.
ACCESSION   DAOTHD010000384 DAOTHD010000000
VERSION     DAOTHD010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64041_length_2069_cov_2.382820"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1573)
                     /locus_tag="P5219_07095"
     CDS             complement(<1..1573)
                     /locus_tag="P5219_07095"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HRX26560.1"
                     /translation="MTDNKKEQERAELHRTIWNIANDLRGSVDGWDFKQYVLGMLFYR
                     YISENLTNYINRGEQEAGNPSFDYGKLSDDEAEEARADIVQTRGFFILPGGLFQNVRK
                     KAAADENLNETLEKVFRSIEASAQGGPSEENFRGLFDDIDVNSNKLGPTVAKRNEKLV
                     KLLNGVGEMKLGDYRDNTIDAFGDAYEFLMGMYASNAGKSGGEYYTPQEVSELLTRIA
                     LVGKTEVNKVYDPACGSGSLLLKFAKILGKENVRLGFFGQEINITTYNLCRINMFLHD
                     IDCDKFDIGHGDTLTDPLHWDDEPFEAIVSNPPYSIKWDGDGNPLLINDPRFSPAGVL
                     APKSKADLAFIMHSLSWLATSGTAAIVCFPGIMYRGGAEQKIRKYLVDNNYVDCVIQL
                     SANLFFGTSIATCIMVLKKSKRDNHTLFIDASQMYVQVTNNNNLTSDHIQRIVGLFTD
                     RANVPYEARLVPNNEVAEQDYNLSVSTYITKEDTREVVDIAELNAEIRQVSARVNELR
                     AQIDAIIAEIDPSNAG"
     gene            1896..>2069
                     /locus_tag="P5219_07100"
     CDS             1896..>2069
                     /locus_tag="P5219_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formiminotransferase"
                     /protein_id="HRX26561.1"
                     /translation="MAGKLIECVPNFSEGRRQDVIESIAANFRGVKGCSLLDYRADAD
                     HNRLVVSLAGEPEA"
BASE COUNT          370 a          531 c          629 g          539 t
ORIGIN      
        1 gcccggcgtt ggagggatcg atctcggcga tgatggcatc gatctgggcg cgcagttcgt
       61 tgaccctggc gctaacctgc ctgatctcgg cgttcaattc agcgatatcc accacctcgc
      121 gggtgtcttc ctttgtgatg taggttgaaa cggaaaggtt gtagtcctgc tctgcaactt
      181 cgttgtttgg caccaggcgg gcttcgtaag gcacattggc acggtcagtg aacaagccta
      241 caatgcgctg aatgtgatca gaggtgaggt tattgttgtt ggtcacctgt acgtacatct
      301 gggaagcgtc aatgaagagg gtgtggttgt cgcgcttgga ttttttgagc accatgatac
      361 aggtagctat gctggtgcca aaaaagaggt ttgcggacaa ctggatgacg cagtcgacgt
      421 agttgttatc caccaggtat ttgcggatct tctgctctgc cccgccgcgg tacatgatgc
      481 cggggaagca gacgatggca gccgtgccgc tggtggcaag ccaggaaagg ctgtgcatga
      541 tgaaggccaa atccgccttc gacttgggcg cgagcacccc cgcgggcgaa aacctcgggt
      601 cgttgatcag gagggggttg ccgtctccgt cccacttgat ggagtagggg ggattggaga
      661 cgatggcctc gaagggctcg tcgtcccagt ggagcgggtc ggtcagtgtg tcgccgtgac
      721 cgatgtcgaa cttgtcgcag tcgatgtcgt ggaggaacat gttgatgcgg cagaggttgt
      781 aggtggtgat gttgatctcc tggccgaaga agccgagacg gacgttttct ttgccgagga
      841 ttttcgcgaa tttcagcagc agggaaccgg agccgcaggc cgggtcgtac accttgttca
      901 cttcggtttt tcccacaagg gcgatgcggg tgaggagttc gctgacctcc tggggggtgt
      961 agtattcgcc gccgctcttg cctgcgttgg aggcgtacat gcccatcagg aactcgtagg
     1021 cgtcgccgaa ggcgtcaatg gtgttgtccc tgtagtcgcc gagcttcatt tctcccacgc
     1081 cgttcaggag cttgacgagc ttctcgtttc tcttcgcgac ggtgggcccg agcttgttgc
     1141 tgttcacgtc gatgtcgtcg aacaggcccc gaaagttctc ctcgctgggg ccgccctgtg
     1201 cggaagcctc gatgctgcgg aagacttttt ccagggtttc gttgaggttt tcgtccgccg
     1261 ccgctttttt tcgtacattt tggaacagcc cgcccggcag gatgaagaag cccctggtct
     1321 ggacaatatc cgcccgggcc tcttccgctt cgtcgtcgct gagtttccca tagtcgaaag
     1381 aagggtttcc ggcttcctgt tcgccgcggt ttatgtagtt cgtgaggttt tcagatatgt
     1441 aacggtaaaa cagcatgccg aggacatact gcttgaagtc ccacccgtcc acgctgcccc
     1501 gaaggtcgtt ggcgatgttc cagatggtgc ggtgcagttc cgcccgttcc tgctcttttt
     1561 tgttgtcggt catggtcgtc cttccttctt cggggctgta gggtgcgaat ttcacgctgc
     1621 cgcagatttg gcgatctgga gcggcgttgg gcgatgcggt attttttttg ctttttcttt
     1681 ccggtcctgc gacatagtat atcgcttttt ttgagcaaat aacaataata tttaatatta
     1741 catcatttac ctgtccgccc ggatttttct tctctaaaac tgctcgaaag agggggcctt
     1801 cccccgtttt acgctttcac ccttcaggtt gtacaaaatt caatgttata atgctcgtga
     1861 gcagcatatc atctccggac cgggagggtg ttcccatggc aggaaaactt atcgagtgcg
     1921 ttccgaattt cagcgaggga cggcggcagg acgtcatcga gtccatcgcc gccaattttc
     1981 ggggagtgaa gggatgctcc cttctcgatt accgggcgga cgccgaccac aaccgcctcg
     2041 tggtgagtct tgccggcgag ccggaagcg
//
LOCUS       DAOTHD010000385         2067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_64142_length_2067_cov_2.250000, whole genome shotgun sequence.
ACCESSION   DAOTHD010000385 DAOTHD010000000
VERSION     DAOTHD010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64142_length_2067_cov_2.250000"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="P5219_07105"
     CDS             complement(<1..321)
                     /locus_tag="P5219_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26562.1"
                     /translation="MEMKRKKPVLAVPLGDAAGIGPEIVAKTAAAGFLEKHARPVIVG
                     DERVLRLGMTIAGKIFPFRSADSLEEAAAEEGLALWNTGSLDAAPLELGTVSPVNGKE
                     EGDNL"
     gene            complement(368..871)
                     /locus_tag="P5219_07110"
     CDS             complement(368..871)
                     /locus_tag="P5219_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRX26563.1"
                     /translation="MNIIRGRVFTFGNNIDTDQIYPGRYLELTEHDEIAAHVMEGADP
                     SFASKMKRGDIIAAGTNFGCGSSREHAVITLKNSGVAAVVARSFARIFFRNAVNLGLL
                     VVEIPDLGDLGLRDGDVGELDVLRGVFAAPGGSRTFAPLPPHVLAIIEAGGIFRLFRE
                     KGAGVFG"
     gene            complement(886..>2067)
                     /locus_tag="P5219_07115"
     CDS             complement(886..>2067)
                     /locus_tag="P5219_07115"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HRX26564.1"
                     /translation="IVLAEVDLAEVNDLYLQVIKSFRELGGEKVRHPEKTSFVFDHYS
                     PAPTIKAADNHKKMREFCAGQGIPHLFDIGEGVCHQVLTESGMVGPGTIVVETDSHTT
                     TMGALGAFGTGVGATDMATILITGKLWFKVPRVMNIVFEGTPPKGVMAKDMILHAIGD
                     LRQDAAIYKAVEFSGGAVREMAQEERFVLCNMAVEMGAKAAFIAPDGTTLEYLSRYGW
                     KGGEIPATDPGYEYDSVVRYDVSSLTPRVALPGSVDAVSAIPGEKITVDQVFLGTCTG
                     GRLNDIRTAAEILAGKRVAPGTRFVVIPASRRIFQDALLAGYIETLVNAGAVLSTPGC
                     GPCLGAHEGILAPGETCVTTSSRNFPGRMGSTEADIYIASPATAAATALMGRLCGADS
                     L"
BASE COUNT          373 a          659 c          627 g          408 t
ORIGIN      
        1 aaggttgtcc ccctcttctt ttccgttgac cggcgagact gtgccaagct caaggggtgc
       61 ggcatcgaga cttcccgtgt tccacagcgc gagcccctcc tctgcggccg cttcctccag
      121 ggaatccgca cttcggaaag gaaaaatttt cccggcaatg gtcatgccca gccggagcac
      181 tctttcatca cccacgatca caggacgggc atgcttttcg aggaatcccg ccgccgccgt
      241 ctttgccact atctcgggac cgatcccggc ggcatctccc aggggaactg ccagaacagg
      301 ctttttccgc ttcatttcca tgaaaaactc ctttctttcg tccgggatcc gcaaaaccgt
      361 ctccgtacta accgaagaca ccggcgccct tttcccggaa caggcggaag atccctcccg
      421 cctcgatgat ggccagcacg tgggggggca ggggcgcgaa ggtcctgctc ccgcccgggg
      481 cggcaaacac gccccggaga acatcgagtt cccctacgtc gccgtcccgg agcccaaggt
      541 ctccaaggtc ggggatctcc accacgagaa gcccgaggtt cacggcattc ctgaaaaaga
      601 ttctggcaaa ggacctggcc accaccgcgg ccactcccga gtttttcagg gtgataacgg
      661 catgctcccg gctggagccg cagccgaagt tggtacccgc cgcgatgatg tccccccgtt
      721 tcattttcga cgcaaaggac gggtccgctc cttccatgac atgggcggcg atttcatcgt
      781 gctcggtgag ttcgagatag cgcccgggat aaatctggtc cgtatcgatg ttgttgccga
      841 aggtaaaaac ccttcccctg atgatgttca tttccctgtc ctcccttaca gcgagtcggc
      901 gccgcaaagc ctgcccatga gcgctgtggc ggctgcggtg gcgggcgacg cgatgtagat
      961 gtccgcctcg gtgcttccca ttcttcccgg gaaattccgg ctcgaggtgg taacacaggt
     1021 ttcccccggc gccaggattc cttcatgggc gccgagacag ggaccgcacc ccggagtgga
     1081 aaggacggcg ccggcgttca ccagcgtttc gatgtatccg gccagaaggg catcctgaaa
     1141 aatccgcctc gaggcgggaa taacgacgaa cctggttccc ggggcgacac gctttcccgc
     1201 caggatctca gcggcggtgc ggatgtcgtt gagccgcccg ccggtgcagg tgcccaggaa
     1261 cacctggtcc acggtgatct tctctccggg aatggcggaa acggcgtcca cactgccggg
     1321 aagggcgact ctcggcgtca gcgaggagac atcgtaccgg accacggagt cgtattcgta
     1381 ccccgggtcg gtcgcgggaa tctccccgcc cttccagcca taccgggaga ggtattcgag
     1441 ggtggtgccg tccggggcga taaaggccgc cttggccccc atctccacgg ccatgttgca
     1501 caggacgaag cgctcctcct gggccatctc acggacagcg ccgccgctga actccaccgc
     1561 cttgtagatg gcggcgtcct gcctgaggtc gccgatggcg tggagaatca tgtccttggc
     1621 catgaccccc ttgggaggag tgccttcgaa gacgatgttc atcacccggg ggaccttgaa
     1681 ccagagcttg ccggtgatca ggatggtggc catgtcggtg gcgcccaccc cagttccgaa
     1741 ggccccgagg gcccccatgg tggtggtatg ggaatcggtc tccacgacga tggtgccggg
     1801 accgaccatg ccgctctcgg tcagtacctg gtggcagact ccctcgccga tgtcgaaaag
     1861 gtgggggatg ccctgcccgg cgcagaattc cctcattttt ttgtggttgt ccgctgcctt
     1921 gatggtgggt gctggtgaat agtgatcgaa gacgaaggat gtcttttcag ggtggcggac
     1981 tttctctccc ccaagctcgc ggaaggattt gatcacctgg aggtagaggt cgttgacctc
     2041 ggcgaggtcc acctccgcca ggacaat
//
LOCUS       DAOTHD010000386         2066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_64169_length_2066_cov_3.437096, whole genome shotgun sequence.
ACCESSION   DAOTHD010000386 DAOTHD010000000
VERSION     DAOTHD010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64169_length_2066_cov_3.437096"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..1620)
                     /gene="hutH"
                     /locus_tag="P5219_07120"
     CDS             complement(76..1620)
                     /gene="hutH"
                     /locus_tag="P5219_07120"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HRX26565.1"
                     /translation="MRTADRNKKVRLDGMSLTLEDVANVARKGWTVELDEETVRRVEA
                     SSAAVRAWENSDDVVYGITTGFGDLASVNISRKDRRLLQENLLKSHACGVGAPFPEDI
                     TRAMMLLRINSLVRGHSGISLAVLNQFVALLNLGIHPVVPCQGSVGASGDLCPLSHLA
                     LPLIGHGSVRYRGRTMTAAKALQMAGLAPVQLGAKEGLALNNGTASMGAMAVLALLDA
                     ECLAKTADIAAALSIEALHGVPFAFDPRTHALRPHNGQGRVAGNIRRLIEGSEILEKF
                     KSGKVQDAYSLRCVPQVHGATRDALGYVRGVLEVEINSVTDNPIIFPDDGVAISGGNF
                     HGQPLALAMDFFGIAVAELASISERRQARLVDTSLSGLPPFLVENSGLNSGFMIAQYT
                     SAALVSENKVLAHPSSVDSIPTSANQEDHVSMGAYSARKGLSILDNARKVVAIELLAG
                     SQALDFSRLLKPGAGTTAAHDCVRGAVPYLKHDEFLQPLIERVEALVSRGAVVKAVEE
                     AIGSLD"
     gene            complement(1617..>2066)
                     /locus_tag="P5219_07125"
     CDS             complement(1617..>2066)
                     /locus_tag="P5219_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRX26566.1"
                     /translation="TMGAVSAEHLLAASDEGIRALAKSGTTAILLPATAYSLRKPYAR
                     ARAMIEHGVPVALATDCNPGSSFTESMPFVFGLAVMAMGLTPEEALTAATKNSACAVG
                     MGAVCGTLEPGKQADFLLLDGETPAILAYHAGVSAVEGVYKKGEQIR"
BASE COUNT          331 a          690 c          691 g          354 t
ORIGIN      
        1 aactgctgat cccctcggaa caaaccataa aaaaacgggg aaggccggca ggccttcccc
       61 ctttttttgc ttctctcagt ccagggagcc tatggcctcc tcaaccgcct tcactaccgc
      121 tccccggctt accagggcct ccaccctctc gataaggggc tggaggaatt catcgtgctt
      181 caggtaggga acggcgcccc ggacgcagtc gtgggcagcc gtggtccccg cgccgggctt
      241 cagcaggcgg ctgaagtcca gggcctggga gcccgccagc agctcgatgg ccaccacctt
      301 ccgggcgttg tcaaggatgg agagcccctt gcgggccgag taggcgccca tggagacgtg
      361 gtcctcctgg ttggccgacg tggggatgga gtccaccgac gacgggtggg ccagcacctt
      421 gttctccgac accagggccg ccgaggtgta ctgggcgatc atgaagccgc tgttcaggcc
      481 gctgttctcc acgaggaagg gaggaaggcc cgacaggctc gtgtccacca gccgtgcctg
      541 gcgccgttcg gaaatgctcg ccagctccgc cacggcgatg ccgaagaagt ccatggccag
      601 ggcgaggggc tgcccgtgga agtttccgcc gctgatggcc accccgtcat cggggaagat
      661 gatgggattg tcggtgacgg agttaatctc cacctcgaga actccccgga cgtagccgag
      721 ggcatcccgg gtggcaccgt ggacctgggg aacgcaccgg agcgagtagg catcctgcac
      781 ctttccggat ttgaactttt ccagtatctc gcttccctcg atgagcctgc ggatgttccc
      841 ggccaccctc ccctggccgt tgtgggggcg gagggcgtgg gtccgggggt caaaggcgaa
      901 gggaacgccg tggagggcct cgatggaaag ggccgccgcg atgtcggcgg tcttggccag
      961 gcactcggcg tccagcaggg caagcaccgc catggcgccc atggaagccg tgccgttgtt
     1021 cagggccagc ccctccttgg ctccgagctg cacgggggcg aggccggcca tctggagcgc
     1081 ctttgccgcc gtcatggtcc gtcccctgta ccgcaccgac ccgtggccga tgagagggag
     1141 ggccaggtgt gacagggggc acaggtcgcc gctggcgccc accgacccct ggcaggggac
     1201 cacgggatgg atgcccaggt tcagcagggc gacgaactgg ttcagaacgg cgaggctgat
     1261 gcccgaatgc ccccggacga ggctgttgat ccgcagcagc atcatggccc tggtgatgtc
     1321 ctcggggaaa ggggccccca cgccgcaggc gtggctcttc agcaggttct cctgcagcag
     1381 ccgccggtcc ttccgggaga tgttcaccga ggcgaggtcg ccgaagccgg tggtgatgcc
     1441 gtagaccacg tcgtcggagt tctcccaggc cctgaccgcc gccgacgagg cctccaccct
     1501 ccggacggtc tcctcgtcca gttccacggt ccagcccttt cgcgccacgt tcgccacgtc
     1561 ctccagggtg agggacatgc cgtcgaggcg cacttttttg ttcctgtccg ccgtcctcac
     1621 cggatctgct cccctttctt gtaaacccct tccacggctg agacacccgc atggtaggcg
     1681 agaatggccg gagtctcccc gtcgaggagc aggaaatccg cctgcttgcc cggctcgagg
     1741 gtcccgcaga ccgcccccat gcccacggca caggcgctgt tcttcgtggc ggccgtcagg
     1801 gcctcctccg gagtgagccc catggccatc accgccaggc cgaagacaaa gggcatggac
     1861 tcggtgaagg acgacccggg gttgcagtcc gtggccagag ccaccggaac cccgtgttcg
     1921 atcatggccc gggcccgggc gtagggtttt ctcaggctgt aggccgtggc cggaaggaga
     1981 atggccgtgg ttccgctttt cgccagtgca cggatcccct cgtcgctcgc ggcgagaagg
     2041 tgctccgccg agacggctcc cattgt
//
LOCUS       DAOTHD010000387         2065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_64206_length_2065_cov_3.912438, whole genome shotgun sequence.
ACCESSION   DAOTHD010000387 DAOTHD010000000
VERSION     DAOTHD010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64206_length_2065_cov_3.912438"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="P5219_07130"
     CDS             <1..539
                     /locus_tag="P5219_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRX26567.1"
                     /translation="PFGAKQKGSSAMPHKRNPILCERISGMSRLLRGYALTAMENMTL
                     WHERDISHSSAERVIWPDAFHLITYMLSKMTYIVKNMVVNTSKMRENLDITRGLVFSQ
                     RVLLDLVERFNLPREEAYAIVQENAMKCWSGRKSFRELLLEDDRVSSRISPEDLGDLF
                     STDHYFRWVDEIFARFRR"
     gene            575..1573
                     /gene="glpX"
                     /locus_tag="P5219_07135"
     CDS             575..1573
                     /gene="glpX"
                     /locus_tag="P5219_07135"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HRX26568.1"
                     /translation="MFAADRNLALELVRATEAAAMAAGRWMGRGDKNAVDGAAVNALR
                     YILNTVSMSGVVVIGEGEKDEAPMLYNGEKLGAEKAPEVDIAVDPIDGTRLTALGLTG
                     AISVVAVSERGTMFNPKNIYYMNKLVVGPEAADVIDINLSPEDNVARVAEAKGKALDD
                     ITVVILDRPRHEELKTRVRSAGARIKLIPDGDIGGALLTCKDQGGADVLMGIGGSPEA
                     VITACAIKCLGGNMQCKLWPRNDEDIAESRKRGLDLDQVLSINDLVRGDNVFFAATGV
                     TDGDFLKGVRYEGREIHTSSMVMRSRSGTIRFVDSIHKQKKLSELSKVSGIEYSTV"
     gene            complement(1630..>2065)
                     /locus_tag="P5219_07140"
     CDS             complement(1630..>2065)
                     /locus_tag="P5219_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HRX26569.1"
                     /translation="GLMEKITFDGQAAMALEGIAEGGQLSAPFTVERREGRFILDWKP
                     AVRWVADNVGTMRKEIIAGAVHTGLALGVAEICGCLAEETGIRDVALSGGVWQNRRLT
                     ATVRKLLGEKGLNSLLHRLVPPNDECVSVGQVAIGAERWKKK"
BASE COUNT          508 a          573 c          558 g          426 t
ORIGIN      
        1 aacccttcgg cgccaagcag aaaggatcga gtgccatgcc ccacaagaga aatcccatcc
       61 tgtgcgaacg catttccgga atgagccgcc ttctgagagg atacgccctc accgccatgg
      121 agaacatgac cctctggcat gaaagggaca tcagccattc ctcggctgag agagtcatct
      181 ggccggatgc ctttcacctg ataacctata tgctctcaaa aatgacatat attgtgaaaa
      241 atatggttgt aaatacttca aaaatgagag aaaatctgga tataacccgg ggactcgtct
      301 tcagccagcg cgttctcctt gacctcgtgg agcgattcaa ccttccaaga gaagaggcat
      361 acgccattgt tcaggaaaac gcgatgaaat gctggtccgg ccggaaatca ttccgggagc
      421 tgcttctgga ggacgaccgg gtttcatccc ggatcagccc ggaagacctg ggagatcttt
      481 tttctacaga tcattatttt cgctgggttg acgagatatt cgcacgtttc cgccggtaac
      541 agggcaacat acaatctatt cgggaggaga tactatgttt gctgctgaca ggaaccttgc
      601 gctcgaactg gtacgggcaa ccgaagccgc cgccatggca gcgggacgat ggatggggcg
      661 gggagacaag aacgccgtcg acggtgcggc ggtaaatgcc ctcagataca ttctcaacac
      721 agtgagcatg tccggcgtgg ttgtgatcgg cgaaggggaa aaagacgaag ctcccatgct
      781 ctacaacgga gagaagcttg gagcggaaaa ggcccctgaa gtcgacatag cggtcgatcc
      841 cattgacgga acccggctca ccgccctcgg gttaaccggt gccataagcg ttgttgccgt
      901 ttctgaacgg ggaaccatgt tcaaccccaa gaacatatac tacatgaaca agctcgtcgt
      961 cggtccggaa gcggctgacg ttatcgacat caacctctct cccgaggata acgtggcccg
     1021 tgttgcagag gccaagggaa aggccctcga tgacataacg gtggtcatcc ttgaccgccc
     1081 gcggcacgag gaactgaaga cccgggtacg gagtgccgga gcaagaataa agcttatacc
     1141 ggacggagac ataggcgggg cccttctcac gtgcaaggac cagggcgggg cggatgttct
     1201 catgggtatc ggcggatctc ccgaagccgt cattaccgcc tgcgctatca agtgcctggg
     1261 aggcaacatg caatgcaagc tgtggccgag aaatgacgag gatatagcgg aaagcagaaa
     1321 acggggcctt gatctggacc aggttctttc catcaacgat ctcgtcaggg gtgacaacgt
     1381 gttcttcgcc gccacgggcg ttaccgacgg agactttttg aagggagtcc gctacgaagg
     1441 acgggagatc cacaccagtt ccatggtcat gagatcgaga agcggcacca tccgttttgt
     1501 tgacagcatc cacaaacaga aaaaactttc ggaactctca aaggtcagcg gcatcgagta
     1561 cagcaccgtc tgataataaa gaacaggaac agcagggcgg ggaaactcac tctctccgcc
     1621 ccgctgtttt tatttctttt tccaccgctc agcccctatg gccacctggc cgacggaaac
     1681 gcattcgtcg tttggaggaa caagccggtg gagcagggaa ttgagtccct tttctccgag
     1741 gagctttcgt accgttgcgg tcagccttct gttctgccag acccctcccg aaagagcaac
     1801 atcccgtatc cccgtttctt cagcaaggca gccgcatatt tctgccactc cgagggcaag
     1861 tccggtatgc accgctccgg caatgatttc cttcctcatc gtgcccacat tatccgcaac
     1921 ccatcggact gccggcttcc agtcaaggat gaaccgtcct tccctccgtt ccacagtaaa
     1981 aggagcactg agctgtccgc cttcggcaat cccttcaaga gccatggcag cctgtccgtc
     2041 aaaggttatt ttttccatga gaccg
//
LOCUS       DAOTHD010000388         2065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_64216_length_2065_cov_3.130846, whole genome shotgun sequence.
ACCESSION   DAOTHD010000388 DAOTHD010000000
VERSION     DAOTHD010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64216_length_2065_cov_3.130846"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="P5219_07145"
     CDS             <1..462
                     /locus_tag="P5219_07145"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRX26570.1"
                     /translation="DISGNSVAWGREYLKDVPNCTLLEGDVHGMEFRSEFDMVLCLQN
                     GLSAIQGDPADTVERSLRALVPGGNAFFSTYAPGFWEWRLAWFREQAEKGLIGELDME
                     RTVNGRIVCKDGFTATTFSEDDLQALGKKTGCPFRTETADGSSLFLIVSKE"
     gene            574..>2065
                     /locus_tag="P5219_07150"
     CDS             574..>2065
                     /locus_tag="P5219_07150"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRX26571.1"
                     /translation="MKSIRFISAMTALVLVLAVSGAAFAAWPEKPVNVIVPWSPGGAS
                     DLTARTLAAEMEKLLGTRLSVTNTPGGAGAIGTQAMFDAARDGYTWSANADGSIVTYQ
                     ALDMLKQSHKDYASFMAVFTPNVICVPANSPFNDLKDLMDAMKARPVTVSSAGTGSGG
                     HQAAEFFRMGTGLEYRHVPYQGGAPAVTATVKGEVEAIMQLSMEVTEMLRAKQLKALA
                     VMDGEPLDVEGYGAIPPITKWLPDFPSVGNRFGFFLPKDIPADAKEAITAAFKTAAAS
                     DAIKKFAADRGSKVVTLYGEEAEKEMEVKASRVCWLLFESGVIKNSPDQYGIPSPKTD
                     HPAGGGRMSPLLFPTLPRGGTPMDKKDLTSGIVLVLTGAAFFAGSLLLPWKDTGYDWF
                     GAPGLVPAILAVLIALCGLRLVIRSRKEPDFYARLAAGENPLLCPEEDEDNDSGGETA
                     RPAWMEKERPRAVLTVALCCTYVLLLGRVPYMAATAAFITGFILLFR"
BASE COUNT          396 a          698 c          621 g          350 t
ORIGIN      
        1 gacatctccg ggaactcggt cgcctggggc agggaatatc tgaaggacgt cccgaactgc
       61 acccttctgg agggagacgt ccacggcatg gagttccgga gtgagttcga catggtcctc
      121 tgcctccaga acggcctctc cgccatacag ggggacccgg ccgacaccgt ggagcggagc
      181 ctcagggccc tcgttccggg aggaaacgcc tttttcagca cctatgcacc gggcttctgg
      241 gagtggaggc tcgcctggtt ccgcgagcag gcagagaaag ggctgatcgg tgagctggac
      301 atggagcgga ccgtcaacgg gcggatcgtc tgcaaagacg gcttcaccgc caccactttt
      361 tcggaagacg atctccaagc cctgggaaaa aagaccggct gtcccttccg cacggaaacg
      421 gcggacggtt caagcctttt cctcatcgtg agcaaggagt gaacttcctg cccggtttcg
      481 ccatgaccgg gggtacaccc gccgttgaca cgggcaaatt ttaaagtgta gaatgttttg
      541 acagatctta ctgcacagga gggattgaag aagatgaaaa gtatccgctt catcagcgcc
      601 atgacggccc ttgtcctggt ccttgcggtt tccggcgcgg ctttcgccgc gtggccggaa
      661 aaacctgtga acgtcatcgt cccatggagc cccggcggag cctcggacct caccgcccgg
      721 accctcgccg ctgaaatgga aaaacttctc ggaacccgcc tctcggtcac caacaccccc
      781 ggcggcgcag gcgccatcgg aacccaggcc atgttcgacg cggcccggga cggctacaca
      841 tggtcggcca acgccgacgg cagcatcgtc acctaccagg ccctcgacat gctgaagcag
      901 agccacaagg actacgcctc cttcatggcg gtcttcacgc ccaacgtcat ctgcgttccc
      961 gccaactcgc ccttcaacga cctgaaggat ctcatggatg ccatgaaggc ccgtccggtc
     1021 accgtctcct cggcgggaac cggcagcggc ggtcaccagg cggcggagtt cttccgcatg
     1081 ggcaccggtc tcgagtaccg gcacgttccc taccagggcg gagcccccgc ggtgacggcc
     1141 accgtcaagg gagaggtgga ggccatcatg cagctctcca tggaagtgac cgaaatgctc
     1201 cgggccaagc agctcaaggc cctggcggtc atggacggcg agcccctcga cgtggagggc
     1261 tacggcgcca tcccgcccat caccaagtgg cttcccgact tccccagcgt ggggaaccgg
     1321 ttcggattct tccttccgaa ggacatcccc gcagacgcga aggaggccat aaccgccgcc
     1381 ttcaagaccg ccgccgcctc ggacgccata aagaagttcg ccgcggacag ggggtccaag
     1441 gtagtgaccc tgtacggaga ggaagcggag aaggagatgg aggtcaaggc ctccagggtc
     1501 tgctggctcc tcttcgaatc cggcgtcatc aagaactccc cggaccagta cggaatccca
     1561 agccctaaaa cagatcaccc ggcaggaggg gggcgcatgt cccccctcct ttttcccacc
     1621 cttccccgcg gaggtacgcc catggataag aaagacctga cgtccggcat cgtccttgtc
     1681 ctcacgggag cggccttttt cgccggcagc ctgcttctgc cctggaagga cacggggtac
     1741 gactggttcg gcgcccccgg cctcgtcccc gccatcctgg ccgtcctcat cgccctgtgc
     1801 ggtctccgcc tggtcatccg gtcccggaag gagcccgatt tctacgcccg gctcgccgca
     1861 ggcgaaaacc ccctgctctg ccccgaagag gacgaagata acgacagcgg aggagagacg
     1921 gcccgcccgg cgtggatgga aaaggaacgg cctcgggcgg tactgaccgt tgccctctgc
     1981 tgcacctacg tgctgctcct cggacgggtg ccctacatgg cggccacggc cgccttcatc
     2041 accggcttca tcctcctctt ccggg
//
LOCUS       DAOTHD010000389         2065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_64222_length_2065_cov_2.733333, whole genome shotgun sequence.
ACCESSION   DAOTHD010000389 DAOTHD010000000
VERSION     DAOTHD010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64222_length_2065_cov_2.733333"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="P5219_07155"
     CDS             complement(<1..565)
                     /locus_tag="P5219_07155"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRX26572.1"
                     /translation="MGIDMLAGPSEVVIVADSSANPVWVAADALARCEHDPNSWTVIV
                     TTSSELAEKTSEEILRQAEVLPTGKLALEAWKNNGRILLADSLEEAVAITDDIAPEHL
                     QVMTENSGETAEHLTNFGSLFIGPYAPVAFGDYVSGPNHILPTVRCARFSNGVYAGTF
                     IKVSSYQEITREGAYSLAEKCAAFADLE"
     gene            complement(520..1221)
                     /locus_tag="P5219_07160"
     CDS             complement(520..1221)
                     /locus_tag="P5219_07160"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRX26573.1"
                     /translation="MKMIVFKEAQKRGAEETASVEKTVRDILDAVRAGGDEAVRNYTE
                     KFDGFRPASLRVDDTTVREAYDKVSPEMVETLQFSALRIRDFALKQKECMKELRWEIS
                     PGVVLGHRLIPVTSCGCYVPAGRYPLPSSALMSAIPAKVAGVKRIAACSPASRGYEGI
                     HPAVLVAMDIAGVDEIYCMGGAQAVGALAFGTETLEPVDIIVGPGNRYVTEAKRQVAD
                     PWELICSPGRAKWSL"
     gene            complement(1218..>2065)
                     /locus_tag="P5219_07165"
     CDS             complement(1218..>2065)
                     /locus_tag="P5219_07165"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HRX26574.1"
                     /translation="KISFRAFSKTDKKKEFSTDMTAFLDQFSLAGKVAVVTGAGRGLG
                     KGYSLGLAAAGAAVVCADIDGTAAGKTAEEIRLSGGRADGIRLDVTKPAEVQECFRSA
                     ADRLGSLDILVNNAGVEEINDFLSVTEEQYDKIMAVNLRGVFFCAQAAARIMVGQGRG
                     KIVNIGSLGSAIGLAKSSVYCGTKGGVVQITKTMAIELARSNIQVNAMGPGYFRTPMT
                     EPFFLDPDHRQWIEERIPAGRVGTAEDLLGTLVFLCSPASDYLTGRLSTLTAAGSRAD
                     LPEGA"
BASE COUNT          418 a          644 c          553 g          450 t
ORIGIN      
        1 cctcgagatc ggcgaaagcg gcgcactttt ccgcaaggga ataagcgccc tcacgggtga
       61 tttcctggta ggagctgacc tttatgaagg ttcccgcata gactccattg gaaaaccgtg
      121 cgcagcgaac ggtcggaaga atgtggttcg ggccggaaac atagtccccg aaggcaaccg
      181 gggcataggg gccgataaag agggaaccga aattagtcag atgttccgca gtctccccgc
      241 tgttttcggt catcacctga aggtgttccg gggcgatgtc gtcggtgatt gccacggctt
      301 cctcaaggga atcggcaagc agtatccttc cgttgttttt ccacgcttcg agggcaagtt
      361 ttcccgtggg aagaacctcc gcctgccgga ggatctcttc ggatgtcttc tccgcaagtt
      421 cactgcttgt ggtcacgata acagtccacg agttcgggtc atgttcacac cgggcgaggg
      481 catctgccgc cacccatacg ggatttgccg aactgtcggc tacaatgacc acttcgctcg
      541 gcccggcgag catatcaatt cccacggatc cgctacctgg cgcttcgctt ccgtaacata
      601 ccggtttccg gggccgacaa taatatccac cggttccaga gtttcagtac cgaaagcgag
      661 ggctcccaca gcctgggctc ctcccatgca gtagatttcg tccactccgg caatatccat
      721 ggccacaaga acggcagggt gaatgccctc ataccccctg ctcgccggag aacaggcagc
      781 tatgcgcttc actcccgcca cctttgccgg aatggccgac atgagggccg aggacggaag
      841 gggatatctg ccggcaggaa cataacaccc gcaggaagtc acaggaatga gcctgtgccc
      901 gaggacaacg ccgggagaaa tttcccagcg gagctctttc atgcactcct tctgcttcag
      961 ggcgaaatcc cggattctca gggctgagaa ctgcagggtc tccaccattt caggggacac
     1021 tttgtcgtag gcttcccgga ccgttgtatc gtcaacccgc agtgaagcgg gccggaaccc
     1081 gtcgaatttc tccgtgtaat ttcgtacggc ttcatcgcct cccgcccgga cggcatcgag
     1141 gatatcccgt accgtctttt ctaccgatgc ggtttcctct gctccgcgtt tttgcgcttc
     1201 cttgaataca atcattttca tgctccttcc ggaaggtcag ctcgcgagcc agccgccgtc
     1261 aatgtagata atctgcctgt aaggtagtcc gaagccggac tgcagagaaa gacgagcgta
     1321 cccagaagat cttcggcagt ccccaccctt cccgccggaa tgcgctcctc gatccactgc
     1381 ctgtggtccg ggtcaaggaa aaagggctcg gtcatgggtg tcctgaagta tccgggtccc
     1441 atggcgttca cctggatgtt cgaccgtgcc agctcgatgg ccatggtctt tgtgatctgc
     1501 accaccccgc ccttggtacc gcagtacacg gaggacttcg ccaggccgat ggcgcttccg
     1561 agagatccga tgttgactat ttttccccgg ccctgcccga ccattatcct ggctgccgcc
     1621 tgggcacaga aaaacactcc cctgaggttg acggccatga tcttgtcgta ctgttcctcc
     1681 gtcaccgata gaaaatcatt gatctcctcc acacccgcgt tattcacaag gatatcgagg
     1741 cttcccagcc tgtccgccgc cgaacggaag cactcctgca cctcggcggg tttcgtcacg
     1801 tcaagacgga tgccatcggc ccttccgccg gagaggcgga tctcttccgc tgtttttccg
     1861 gcggccgttc cgtcgatatc agcgcacacc acggcagcgc ccgccgcagc aagtcccagc
     1921 gaatacccct tcccaaggcc gcggcccgcg ccggtcacaa ccgcaacttt gccggcgagg
     1981 gagaactgat cgagaaatgc cgtcatgtca gtcgaaaact cctttttttt gtcagttttt
     2041 gaaaaagctc tgaaggatat cttcc
//
LOCUS       DAOTHD010000390         2061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_64393_length_2061_cov_3.159023, whole genome shotgun sequence.
ACCESSION   DAOTHD010000390 DAOTHD010000000
VERSION     DAOTHD010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64393_length_2061_cov_3.159023"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="P5219_07170"
     CDS             <1..942
                     /locus_tag="P5219_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HRX26575.1"
                     /translation="RKILKLGPRMLIGFFSATITIMIGFVVMFALMKNGFPPEAWKTW
                     GALAGSWIGGTANMVAIQGALGIEESAMGYTLLVDNVNYSIWVIMLLATVPYAKFFNK
                     WTRTDTSTIDSVGLSLSELDENARTKMETSDLALLIGAGLLVSALSSWLATFISPMLM
                     DVFGKSDFLSVSTVTIVLATIFGIVCAMTPMNSIPGSSPVSMTMLYIMICLIASRASF
                     KELTDAPYYLAAGFVVLGIHALLMAIIAKILKIDLFTCAVASLANIGAVAAAPIIAAA
                     YKETLVPIGVLMALMGYVAGTAGGLFVAKMLSMMAGV"
     gene            944..2032
                     /locus_tag="P5219_07175"
     CDS             944..2032
                     /locus_tag="P5219_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HRX26576.1"
                     /translation="MKIRTLRLGRLSVPLKKPFKTALRTVTEVTTNVVVVETDEGVCG
                     YGEAPPTAVITGDSNGAIRGAVEERLRPLLAGQDTENLNDLLDIVDSALVHNTSAKAA
                     VDIALHDLWGKILGKPLYALLGGTAKEVETDYTISVNDPETMVKDSLEAVADGYPALK
                     IKVGTDSAQDMIRLREIRSAVGKDILLRVDANQGWTPKQAVRTIRFFEDEGLDIELVE
                     QPVKAHDFEGLKYVTDSVETLILADESVFSPRDAFTILSMRAADLVNIKLMKTGGIRN
                     ALAVCAMARSCGVECMIGAMMETKISVTAAAHLASALPVITRADLDPPILCASDPVEG
                     GAVYTGAKVAPSAGPGLGITNIRGLVLE"
BASE COUNT          406 a          638 c          621 g          396 t
ORIGIN      
        1 cggaagatcc tgaaactcgg ccccaggatg ctcatcggat tcttctccgc caccatcacc
       61 atcatgatcg gcttcgtggt catgttcgcc ctcatgaaga acggctttcc tcctgaggca
      121 tggaagacct ggggagccct tgccggaagc tggatcggcg gcacggcaaa catggtggcc
      181 attcagggag ccctggggat cgaagaatcc gccatggggt ataccctgct cgtggacaac
      241 gtgaactatt ccatctgggt cattatgctg ctcgcgaccg ttccctacgc caagttcttc
      301 aacaagtgga ccaggaccga cacctccacc atcgattcag tgggtctttc cctttcggaa
      361 ctggatgaaa acgcccgaac gaagatggag acgtcggatc tcgcccttct catcggagcc
      421 ggccttctcg tttcggccct ctcctcctgg ctggcaacct ttatttcgcc catgctgatg
      481 gatgtcttcg gcaagagtga cttcctctcc gtcagtaccg tcaccatcgt cctggccacc
      541 atcttcggca ttgtctgcgc catgacgccc atgaacagca ttcccggttc ctcgcccgtt
      601 tccatgacca tgctctacat catgatctgc ctcatcgctt ccagggcatc cttcaaggag
      661 ctgactgacg cgccctacta cctggccgca ggcttcgtcg tcctgggcat ccacgctctt
      721 ctcatggcca tcatcgccaa gattctcaag atcgacctct tcacctgcgc cgtggcaagc
      781 cttgcgaaca tcggcgccgt ggcggcggcc cccatcatcg cggcagccta caaggaaacc
      841 ctggttccca tcggcgtcct gatggctctc atgggatacg tggcgggcac cgcgggagga
      901 ctcttcgtcg ccaagatgct gtccatgatg gcgggagtct agaatgaaaa tacgcacact
      961 ccgtctcgga cgtctttccg ttccgctgaa aaagccgttc aagacggcgc tccggacggt
     1021 cactgaagtg accaccaacg tggtggtcgt ggagacggat gaaggagtct gcgggtacgg
     1081 agaagcccct cccacggcgg tcattacggg ggattccaac ggagcgatcc gtggtgcggt
     1141 ggaggagcgg cttcgtccgc tccttgccgg gcaggatacg gaaaacctca acgatctgct
     1201 ggacatcgtg gattcggcac tggtgcacaa cacgtcggca aaggccgcgg tagacatcgc
     1261 cctccacgac ctgtggggaa agattctcgg gaagcctttg tacgccctcc tcgggggaac
     1321 ggcgaaggaa gtggagacgg actacaccat cagcgtcaac gatccggaaa ccatggtgaa
     1381 agacagcctc gaggccgtgg cggacgggta tcccgccctg aagatcaagg tggggaccga
     1441 ctcggcccag gacatgatcc gcctcaggga gatccgttcg gcggtgggga aggacattct
     1501 gctccgggtg gacgccaacc aggggtggac gcccaagcag gcggtccgca ccatccggtt
     1561 cttcgaggac gaggggctgg acatcgagct cgtggagcag ccggtgaagg cccatgactt
     1621 cgaggggctg aaatatgtca ccgacagcgt ggagacgctg attcttgcgg acgagtcggt
     1681 gttttcgccc cgggacgcct tcaccatcct ctccatgagg gcggcggacc tggtcaacat
     1741 caagctcatg aagaccgggg gcatacggaa tgccctggcc gtctgcgcca tggcccgctc
     1801 ctgcggcgtg gagtgcatga tcggggccat gatggagacg aagatcagcg tcacggcggc
     1861 ggcacacctt gcctccgccc tgccggtcat cacaagggcg gacctggatc cccccatcct
     1921 ctgcgcctca gacccggtgg aaggcggcgc ggtctacaca ggagcgaagg tcgctccttc
     1981 agcgggaccc gggctgggga tcacgaacat caggggactc gtgctggaat aggaattcca
     2041 gagaaggcgc agggagcatg c
//
LOCUS       DAOTHD010000391         2061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_64401_length_2061_cov_2.893819, whole genome shotgun sequence.
ACCESSION   DAOTHD010000391 DAOTHD010000000
VERSION     DAOTHD010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64401_length_2061_cov_2.893819"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="P5219_07180"
     CDS             <1..220
                     /locus_tag="P5219_07180"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRX26577.1"
                     /translation="PMSLFSLGLRKIYLRDAYTISLAEPLTACVLSALILGERLTPVS
                     IGGAALILCGILLLPVKETVKEPAEGTA"
     gene            217..1392
                     /locus_tag="P5219_07185"
     CDS             217..1392
                     /locus_tag="P5219_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRX26578.1"
                     /translation="MSTFDMKEDSMTGLAQVFTSGNEAIAEGAIAAGASFYAGYPITP
                     SSEIAETAARRLPETGGMYVQMEDELSGMAAIVGASVSGARAFTATSGPGFSLMQENL
                     GMAVMGEIPCVVVNVQRSGPSTGMATKPAQGDVMQARWGTHGDHGIITLAPSSVQDCY
                     DFTIRAFSLADRFRTPVILLADAAVAKLKENVRFRSPTPEERAPRPLPDCPPEAYRPF
                     AVDPEEKVITPLPPFGSDYIFRVTGSMHDETGGQGASPANADRVIRHLAGKIEKNRDA
                     IVRVRRFFLEDAEWAVIAFGCSARSARAAVREARRQGIPAGLLELTTLWPFPDDDVRD
                     VLSRVRGAVVPELNLGQAAGEVRRLNDFGIPVRGISRVDGTLIPPGDILSALREARS"
     gene            1389..>2061
                     /locus_tag="P5219_07190"
     CDS             1389..>2061
                     /locus_tag="P5219_07190"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRX26579.1"
                     /translation="MTARDCREYLAENRLPTIWCPGCGNGLVLKAIAGAFADLGLDRR
                     KTAAVSGIGCWGWADDYLAVNAFHGTHGRALAFATGIKLANPELTVVVLMGDGDGATI
                     GGNHLIHAARRNVDLTAVVVNNYNYGMTGGQYSGTTPRGSITSTSPLGHVEDPFDLCA
                     LTAAAGAPFVARGSVCGGENLRVLIRRGLEHRGFSFVEAASVCPTHFGRRNGLRTPAE
                     AFSWLR"
BASE COUNT          340 a          728 c          663 g          330 t
ORIGIN      
        1 tcccatgagc ctcttttccc tcgggctccg aaaaatctac ctcagggacg cctacaccat
       61 ctctctcgcc gaacccctca cggcctgcgt cctctccgcc ctcatcctgg gagagcggct
      121 caccccggtc tccatcggcg gagccgccct gatcctctgc ggcatcctgc tgcttcccgt
      181 caaggagacg gtgaaggaac cggcggaagg aacggcatga gcactttcga catgaaggag
      241 gattccatga ccggactcgc gcaggttttc acgtcaggaa acgaggccat cgcggaaggc
      301 gctatcgcgg cgggggcatc gttttacgca ggctacccca tcaccccgtc gtcggaaatc
      361 gccgagactg ccgccaggcg gcttcctgag accgggggca tgtacgtcca gatggaggac
      421 gagctgtcgg gcatggccgc catcgtaggt gcgtccgttt ccggggcgag agctttcacc
      481 gccaccagcg gcccgggctt ctccctgatg caggagaacc tcggcatggc cgtcatgggg
      541 gagatcccct gcgtggtggt gaacgtccag cgctcgggcc cctccaccgg catggccacg
      601 aaacccgccc agggggacgt gatgcaggcc cggtggggta cccacgggga tcacggcatc
      661 atcaccctgg ccccgtcgtc ggtgcaggac tgttacgact tcacgatccg ggccttctcc
      721 ctggccgacc ggttccgaac gccggtgatc ctgctcgccg acgcggcggt ggccaagctg
      781 aaggaaaacg ttcgcttccg ctccccaaca cctgaagaga gggccccgcg gcccctgccg
      841 gactgccccc ccgaagcgta ccggcccttc gccgtggatc cggaggagaa ggtcatcaca
      901 ccccttcccc ccttcgggag cgactacatc ttccgcgtca caggctccat gcacgatgaa
      961 acgggaggcc agggagcctc cccggcgaac gccgacaggg tgatccgcca tcttgcgggc
     1021 aagatcgaaa agaaccggga tgccatcgtc cgggtccggc gcttcttcct cgaggacgcg
     1081 gaatgggccg tcatcgcttt cggatgctcc gccaggtccg cccgggccgc cgtgagggag
     1141 gccaggagac agggcattcc cgcggggctc ctggagctca ccactctctg gcccttcccc
     1201 gacgacgacg tgcgggacgt tctctcccgg gtcaggggcg ccgtggtgcc ggagctgaac
     1261 ctcggccagg ccgcggggga agtccggcgg ctcaacgatt tcggcatacc cgtcagggga
     1321 atcagccggg tggacggcac cctcatcccc cccggggata tcctctccgc tctcagggag
     1381 gcccggtcat gacggcccgg gactgtaggg aatacctcgc ggaaaaccgg ctgcccacta
     1441 tctggtgccc cggctgcggc aacggcctcg tcctgaaggc catcgccggt gcctttgccg
     1501 acctcgggct ggaccgcagg aagaccgccg ccgtctcggg catcggatgc tggggatggg
     1561 cggacgacta cctggcggtg aacgccttcc acgggaccca cgggcgggct ctcgccttcg
     1621 ccacggggat caagctcgcc aacccggaac tcaccgtggt ggtcctcatg ggcgacggcg
     1681 acggcgccac catcggaggc aaccacctga tccacgccgc caggaggaac gtggacctca
     1741 cggcggtggt ggtgaacaac tacaactacg gcatgacggg aggacagtac tcgggcacca
     1801 ccccccgggg aagcattaca tccacctcgc ccctgggcca cgtggaggac cccttcgatc
     1861 tctgcgctct tacggccgct gcgggtgctc ccttcgtcgc ccggggatcg gtgtgcggcg
     1921 gagaaaacct ccgcgtcctc atccgccggg gcctggaaca caggggattc tccttcgtgg
     1981 aggcggcgtc cgtctgcccc acccatttcg gccggaggaa cggcctgcgg acgccggccg
     2041 aagccttctc ttggctccgg g
//
LOCUS       DAOTHD010000392         2047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_64978_length_2047_cov_3.721888, whole genome shotgun sequence.
ACCESSION   DAOTHD010000392 DAOTHD010000000
VERSION     DAOTHD010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64978_length_2047_cov_3.721888"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="P5219_07195"
     CDS             <1..507
                     /locus_tag="P5219_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRX26580.1"
                     /translation="DPLEPAVVSLGEVHAGTAFNIIPGECRITGTVRALNQPTREFLA
                     RRIEEVASGVASSMRGNIEFKYGWEGPAPVVNDPAMTEELRLAAEKIVGPEKVREISR
                     PSMGGEDIAFFLEKAPGTFFFLPGCSEAKGQTWPHHNSRFDIDEDILWIGPAVMATMA
                     FDWLKKQA"
     gene            566..1657
                     /locus_tag="P5219_07200"
     CDS             566..1657
                     /locus_tag="P5219_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HRX26581.1"
                     /translation="MLKERLKELLRELTAIPGVSGDERKIIAVLADKLRPLADELLVD
                     RWGNIIAVRKGGLPGPKVMAAAHADEIGLCVKNILPNGFLLMSRIGVVSDLLLQGRRV
                     RINGTIPGILGIKAGHLMSAEERTKVKPMNECYIDVGAFSREEALAMGIRVGDRINFE
                     GDFMEMTNPDLICSKAVDDRIGCSILIALFEELRGTSFPGTLCGVITVQEEIGLRGAA
                     MVSGRVAPDYAVALDTIPSGDTPDVSYDRQLPVRLGGGPAIALLDGYDSAFIANVVHP
                     KVRRILEEAAERAAVPLQMVTLGGEVYTTDATNISLESHGIPVGVLLTPRRYSHSPVE
                     LVNLNDAAGAVQVLKEVMAGNGSVSLDFI"
BASE COUNT          424 a          597 c          593 g          433 t
ORIGIN      
        1 gatcctctcg aaccggcagt cgtcagcctc ggcgaagttc acgcgggaac ggccttcaac
       61 atcatccccg gggagtgtcg aatcaccggc acggtgaggg ccctcaacca gccgacccgg
      121 gaattcctgg cccgccgcat cgaggaggtc gcttccggcg tcgcctcgag catgagggga
      181 aacatcgagt tcaaatacgg atgggagggc cccgctcccg tggtgaacga ccccgccatg
      241 accgaggaac tcaggctcgc cgcggagaag atcgtggggc cggaaaaggt cagggaaatt
      301 tcccggccct ccatgggagg agaggacatc gccttcttcc tcgaaaaagc cccgggaacc
      361 ttcttcttcc ttcccgggtg cagcgaggcc aagggacaga cctggcccca ccacaacagc
      421 aggttcgaca tcgacgagga catcctctgg atagggcctg ccgtgatggc caccatggcc
      481 ttcgactggc tgaaaaaaca ggcttgatcg cttcggggag cccttcaggg ctccccgcgc
      541 tcttttcctg aaaggggaga attccatgct gaaggagaga ctcaaagagt tgctcaggga
      601 actcaccgcc atcccgggag tgtccggcga tgagcggaaa atcatcgccg ttctggccga
      661 caagctccgt cccctggcgg atgaactctt ggtggacagg tgggggaaca tcattgcggt
      721 caggaaaggc gggctgccgg gaccgaaggt gatggcggcg gcccatgcgg acgaaatcgg
      781 cctgtgcgtg aagaacatcc ttccgaacgg attcctcctc atgagccgca tcggcgttgt
      841 cagcgacctt cttctccagg gacggagagt gaggatcaac ggcaccatcc ccggaatcct
      901 gggaatcaag gcggggcacc tcatgagcgc cgaggagcgc acgaaggtca agcccatgaa
      961 cgaatgctac atcgacgtag gggcattttc cagggaggaa gccctggcca tgggcatccg
     1021 ggtgggggac cgcatcaact tcgaagggga cttcatggaa atgacgaatc ccgaccttat
     1081 ctgctcgaag gctgttgacg acaggatagg atgctcaatt ctcatcgccc ttttcgagga
     1141 acttcgggga acatccttcc ccggaaccct gtgcggcgtc attacagtcc aggaagagat
     1201 cgggctccgg ggggcggcca tggtgagcgg cagggttgcc cccgactacg ctgtcgccct
     1261 cgacaccatc cccagcggag acacgcctga cgtgagctat gaccgccagc ttccggtgag
     1321 actgggcggg gggcccgcca ttgcccttct ggacggctac gattcagcct tcatcgccaa
     1381 cgttgtccac ccgaaagtga ggcggatact cgaagaggct gcggaaaggg ccgcggtgcc
     1441 ccttcagatg gtcaccctcg gaggcgaggt atataccacc gacgccacaa acatctctct
     1501 cgaatcccat ggaataccgg tcggcgtcct tctgaccccc cgaaggtatt cccactcccc
     1561 cgtggagctg gtgaacctga acgatgcggc aggggccgta caggtgctca aggaagttat
     1621 ggcaggaaac ggttctgtct ctctggattt catctgatca caggggaaca tggaaccttt
     1681 ttgggatttc tttcacgata ggtactttta cctactttac aaatgtgttt ttgggtgata
     1741 gcataacgaa tacagtattt tggatacaca actttttgca cctcctcctt tttcttcctt
     1801 ggagaaagag gatcagcccg gtttccgggc agaaacattc attccctgaa tcacggggcc
     1861 ggtttcccca accggccccg cctccttttt ccgccccttt ccctggtata atcgatccat
     1921 ttcctgttta tttcttcaat aaactggaaa tgtgcctcag gcactgctgg tacctatgtt
     1981 ctgccgggcg gctgtgttgt tttcggtgtt ggttgttttc tgtttttttc tccgttaact
     2041 taccttt
//
LOCUS       DAOTHD010000393         2044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_65116_length_2044_cov_3.163399, whole genome shotgun sequence.
ACCESSION   DAOTHD010000393 DAOTHD010000000
VERSION     DAOTHD010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65116_length_2044_cov_3.163399"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="P5219_07205"
     CDS             <1..852
                     /locus_tag="P5219_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRX26582.1"
                     /translation="RGAEVIPADIPWGRGATPDEVKDALARSPDPAVVLFTHNETSTG
                     AVAPLGECIAALPHDGPLVLVDAVSSLGALPCFPEEWGIDGLASCSQKGLLTPPGIAV
                     AALSPRGWARAGSRPCPSYYFDLSLHRRFLEKERPQNPYTPPVTLLCSLAEALRFLED
                     FGFGKWFRLKARTAETFETACKAMGLPLFVEDPAFRSPAVTAVKVPGGKGKEMKHALE
                     TLGVEAAAGQGKEPSLLRVAHYSPGGWPELSLIAGSIYGAGKMCGLDLRPDFSEAAWN
                     CWTREGE"
     gene            849..>2044
                     /locus_tag="P5219_07210"
     CDS             849..>2044
                     /locus_tag="P5219_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRX26583.1"
                     /translation="MTEMKVLVTDTVSEAGISLLRKEKDLQVEVRLGLSREELLEAVA
                     DADGMLTRSGTPLDGEVLGAAKKLRAVARAGVGVDNIDLAAASRRGIVVINAPTGNTL
                     AATEHTLAMMLSLVRKVPQAWNSLASGEWKRSRFMGMQLSGKRLLVIGLGRIGTQVAL
                     RCRAFGMEVSAYDPYISKEKADRAGVRLLDDLSGALSLADVVTLHVPLTAETRRLLDG
                     RLLKACKRGAFLVNCARGGLVDEEAVADGVRSGLLAGAAFDVFDGEPVRTDHPLLAPD
                     IREKVILTPHIGANTEEAQSAVAVIAASNLAAALLGRPYEHAVNLPFSEQLLSDGRRA
                     YLSLARKMGYLAAHLLREPVKEIRVTLRGPLFSKGDDPICFEVPYHYSPFTVAGLKGM
                     LEVSHGP"
BASE COUNT          341 a          698 c          665 g          340 t
ORIGIN      
        1 cggggagccg aggtcattcc cgcagacatt ccctgggggc ggggcgccac cccggacgag
       61 gtgaaggacg ccctggctcg gagccccgat cccgccgtcg ttcttttcac ccacaacgag
      121 acctccaccg gcgccgtcgc gcctctcggg gagtgcatcg ccgcccttcc tcacgacggg
      181 cccctcgtgc tcgtggacgc cgtcagctct ctcggcgcgc tgccctgctt tcccgaagaa
      241 tggggcatcg acggccttgc ctcctgctcc cagaagggac tgctcacacc tccgggcatc
      301 gccgtggcgg ccctgtctcc ccggggctgg gcgagggcgg gatcccgccc ctgcccttcc
      361 tactatttcg acctttcact gcaccggagg ttcctggaga aagaacggcc ccagaacccc
      421 tacacccctc cggtcaccct gctctgcagt cttgccgaag cccttcggtt cctggaggac
      481 ttcggcttcg ggaaatggtt ccggctgaag gccagaacgg ccgaaacctt tgagaccgcc
      541 tgtaaggcca tgggccttcc cctcttcgtg gaggaccctg ccttccgctc ccccgccgtc
      601 acggcggtga aggtgcccgg aggaaaggga aaggaaatga agcatgccct ggaaactctc
      661 ggggtggagg ccgccgccgg acagggaaag gaaccctccc tgctccgggt ggcccattac
      721 agcccgggag gatggcctga actctcactt atcgccggaa gcatctacgg agccgggaag
      781 atgtgcggcc tggaccttcg gcccgatttt tcggaagctg cctggaactg ctggacaagg
      841 gaaggggaat gacggaaatg aaagtgctcg tgacggatac cgtatcggaa gcgggaatca
      901 gtctgctccg gaaggagaag gacctccagg tggaggtgcg gctcggcctc tccagggaag
      961 aactgctgga ggcggtggcc gatgcggacg gcatgctgac caggagcggt acgcccctcg
     1021 acggggaggt gcttggagcg gcgaagaaac tccgggccgt ggccagggca ggtgtcggcg
     1081 tggacaacat cgacctagcg gcggcgagcc gccgggggat cgtggtgatc aacgccccca
     1141 ccggaaacac tctcgcggcg acggagcaca ctctggccat gatgctctcc cttgtgcgga
     1201 aggtacccca ggcgtggaac tccctggcgt cgggcgaatg gaagcggagc cggttcatgg
     1261 gcatgcagct ctcgggaaag cggcttctgg tcatcggcct cgggcggatc gggacccagg
     1321 tggccctgcg gtgccgggcc ttcggcatgg aggtgtccgc ctacgacccc tacatatcga
     1381 aggaaaaggc ggaccgcgcg ggggtccggc tcctggatga cctttccggg gctctttccc
     1441 ttgccgacgt ggtcaccctc cacgtccctc tcaccgccga gaccaggcgg ctgctcgacg
     1501 gccggcttct caaagcctgc aagcggggag ccttcctggt gaactgcgcc cggggcggcc
     1561 tggtggacga agaagccgtc gcagacggcg tacgctccgg ccttctcgcc ggggccgcct
     1621 tcgacgtgtt cgacggcgag ccggtccgaa cggaccatcc cctcctcgca ccggacatcc
     1681 gggagaaagt catcctcacc ccccacatcg gggccaacac ggaagaggca cagtcagccg
     1741 tggcggtgat cgccgcatcc aatctcgctg ccgctctcct gggcaggccc tatgagcacg
     1801 cggtgaacct tcccttctcc gagcagcttc tctccgacgg gcggagggcc tatctctccc
     1861 tcgcccggaa gatggggtac ctcgccgccc accttctccg ggagccggtg aaggagatcc
     1921 gggtcaccct ccgggggccc ctcttctcga aaggggacga ccccatctgc ttcgaagtgc
     1981 cctaccacta ctctcccttc accgtggcgg ggctcaaggg catgctcgaa gtctcccacg
     2041 ggcc
//
LOCUS       DAOTHD010000394         2043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_65171_length_2043_cov_2.673541, whole genome shotgun sequence.
ACCESSION   DAOTHD010000394 DAOTHD010000000
VERSION     DAOTHD010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65171_length_2043_cov_2.673541"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..156)
                     /locus_tag="P5219_07215"
     CDS             complement(<1..156)
                     /locus_tag="P5219_07215"
                     /inference="COORDINATES: protein motif:HMM:NF014857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase family
                     protein"
                     /protein_id="HRX26584.1"
                     /translation="MTNVLVIGSGAREHAIVWALSKSGAVGTVHAAPGNAGMAPLAVL
                     HPSTGLTA"
     gene            complement(171..1694)
                     /gene="purH"
                     /locus_tag="P5219_07220"
     CDS             complement(171..1694)
                     /gene="purH"
                     /locus_tag="P5219_07220"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRX26585.1"
                     /translation="MKGKKALLSVFDKTGIVPFAEGLVSLGWELLSSSGTADHLRKAG
                     LAVTEVSDVTGYPHILGGRVKTLHPLVFGGILARRDVEEDMKETEQFGIPLLDMIVCN
                     LYPFEETARRDPELDELLENIDIGGVSLLRAAAKNYRQVVVLTDPADYGSIVAELRSE
                     GDVSQETRQMLAVKAFRSTSAYDAAIVDGLAEATGSTPSCLPDKMPLAFVKKQDLRYG
                     ENPHQEASLYLPSLADLPWEQISGKPLSYNNILDTDCAMRGCALLQDCCGALVIKHTT
                     PCGMACGSSPREAYEKAVGCDPVSAFGGVVGISKKVDMETVLSIADRFTEVLVAPDYD
                     DQTVEILREKKPSLRVLRWKGGRVSPLQFTGTWSGLLVQQDSLPPLPDKGEWIGKPRP
                     DLWEDLLFAWKVAALSKSNAISIVKNREAVGIGRGFCSRLHAVDFAVRQAGEKARGAV
                     LGSDAFFPFSDGVEAAADAGIEAIIQPGGSVRDSEVFSSAEKLGISMFISGWRTFRH"
     gene            complement(1699..>2043)
                     /locus_tag="P5219_07225"
     CDS             complement(1699..>2043)
                     /locus_tag="P5219_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HRX26586.1"
                     /translation="EWIVLAGFMRILSPEFVAAHRGRIVNIHPSLLPSFPGRDGIGDA
                     WKYGVKITGVTVHLVDEGIDSGPILAQKAVPVRPADTPESLEKRIHRVEHGLYWKTLA
                     ALFSGVTVSERS"
BASE COUNT          396 a          589 c          623 g          435 t
ORIGIN      
        1 ggcggtcagg ccggttgaag ggtgaagaac agcgagaggt gccatgccgg catttccggg
       61 tgccgcatga acggttccca cagcccctga tttcgaaagg gcccacacta tggcgtgttc
      121 cctggccccg ctgccgataa ccaggacgtt tgtcatagcc ctttgccccc tcaatggcgg
      181 aatgtgcgcc atccgctgat gaacatggaa atgccgagct tttcggcgga tgagaagact
      241 tcgctgtccc gtactgatcc gccgggctgg atgatcgcct cgatgcctgc gtccgcagcc
      301 gcctcgacac cgtcggagaa ggggaagaag gcatcggagc ccagaaccgc tccccgtgcc
      361 ttttctcccg cctgcctcac ggcaaagtca actgcgtgca gcctgctgca gaaccctctt
      421 cctataccca ccgcttcccg gttcttcacg atggagatgg cgttgctctt ggaaagggcg
      481 gccactttcc acgcaaagag aaggtcttcc cagaggtcag gccgcggctt tcctatccat
      541 tcgcccttat cgggaagggg aggaaggctg tcctgctgga ccaggaggcc gctccaggtt
      601 ccggtgaact gcaggggcga taccctgccg cctttccatc ggagcaccct gagggagggc
      661 ttcttctccc tgaggatttc caccgtttga tcgtcataat caggggcgac gagcacctcg
      721 gtgaaccggt cagctatgga gagcaccgtt tccatgtcca cttttttcga tatacccacc
      781 actcctccga aagccgaaac aggatcgcat cccaccgcct tctcgtaggc ttcccgcgga
      841 gaggaaccgc aggccatgcc gcagggggtt gtgtgtttga tcaccagggc tccgcagcag
      901 tcctggagga gggcgcatcc tctcatggcg cagtcggtgt cgaggatatt gttgtaggat
      961 aggggcttgc cggagatctg ttcccacggc aggtcagcca gggagggcag gtaaagggac
     1021 gcttcctggt gggggttttc accgtaccgg agatcctgct tcttcacaaa ggcaaggggc
     1081 attttgtcag gaagacaaga gggagtgctg ccggtcgcct ccgcaagtcc gtcgacgatg
     1141 gcggcatcgt aggcagaggt tgagcggaag gcctttactg ccagcatctg cctggtttcc
     1201 tgggaaacat ctccttcgga ccggagttcg gccacgatgc tgccgtaatc ggcggggtcc
     1261 gtcaacacga cgacctgccg gtagtttttg gcggcggctc tcaaaaggga aactcccccg
     1321 atatcgatgt tctcaagaag ctcgtccagc tcgggatcac gtcgggctgt ctcttcgaag
     1381 gggtagaggt tgcagacgat catgtcgagc aggggaatgc cgaactgttc cgtctccttc
     1441 atgtcttcct caacgtccct ccgggcgaga attcctccga agacgagggg gtggagggtt
     1501 ttcaccctgc ctccgagaat atgggggtac ccggtgacgt cggagacttc tgtgacggcc
     1561 agaccggctt tcctgagatg atcggccgtt ccggaacttg aaagcagttc ccagcccagg
     1621 gagacaagtc cttccgcgaa cgggacgatt cccgtcttgt cgaaaacgga gagaagcgct
     1681 tttttgcctt tcattgtttc agctcctttc agatacggtg actccggaaa aaagtgccgc
     1741 gagggttttc cagtagagtc cgtgctccac cctgtggatt cgcttctcca gggattctgg
     1801 tgtatcggcg ggtcggacgg gaactgcctt ctgggccaga atggggccgg aatcgatgcc
     1861 ttcgtccacc aggtggaccg ttactccggt aattttgacc ccgtacttcc atgcatcccc
     1921 tatgccgtcc cggcctggaa aagagggaag aagagagggg tggatattca cgatacggcc
     1981 tctgtgggcg gcaacgaatt ctggagagag gatgcgcatg aagccggcga ggacaatcca
     2041 ctc
//
LOCUS       DAOTHD010000395         2033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_65586_length_2033_cov_3.206775, whole genome shotgun sequence.
ACCESSION   DAOTHD010000395 DAOTHD010000000
VERSION     DAOTHD010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65586_length_2033_cov_3.206775"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /locus_tag="P5219_07230"
     CDS             <1..1396
                     /locus_tag="P5219_07230"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HRX26587.1"
                     /translation="AELVLFADDGTPKPGNVASAALVSLVLGEQEELWNAFEAPIDFL
                     VGASNTGGTRIYRRLAEERMGTLAEAHAGLADEKVLSALAEEIKKTVPGPLIQSTPGG
                     DDGTKDFASRLPVFRMSGKRFTPDAYVMNMLTSPRVGTDANPRNLPEGTDVMAALGSK
                     AAVGLAAKNNGVKGYAEALKKLKTWINEHLGGEETVYSLWIKAFHEGFRDSGSDQFFY
                     RSPAWQWKKLSTFSASWAELKHDTVLYAEQSGAEMGDGGDTEAGPFAPPQPKGYVEPD
                     PRTFDALLSAVTRLKAFIAEFGMEPEDEEFEKEGIPYASRLETLAELLTIARDIAAKE
                     TGGTLLTAEDYGNIKYLARAFNAQLLLPGEMVEDTKQLRMALVTDIAADYFEGRVLHI
                     GSGRPQRIHVFVHDASGGPRVTRGFIFSYYEFPRSLGDGRMTDEEWKALVYNDGRSGE
                     LKQYHPAWYEELRK"
     gene            1377..>2033
                     /gene="amrB"
                     /locus_tag="P5219_07235"
     CDS             1377..>2033
                     /gene="amrB"
                     /locus_tag="P5219_07235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HRX26588.1"
                     /translation="MKSFGNKFFPAVLLLCVLSAAGAIPQKAEGGAELRSAFPSGKGA
                     GEREGPARIIGGIVPHHDLALGMIRRFYERLGSPDVRRVWLLSPDHFRRARTFAALCP
                     DDWKTPEGILRADREACETLPSLSVAGADGALFRREHGITIHIPFIARHFPNASVVPL
                     VIGARTPDLALVILKNAVRDLLRDGDAVILSMDLSHYKTPEAMAAEDRKTLDVLAELR
                     P"
BASE COUNT          377 a          666 c          650 g          340 t
ORIGIN      
        1 cgccgagctt gtcctcttcg ccgacgacgg cacgccgaag cccgggaacg tcgcgtcggc
       61 ggccctggtg tccctggttc tcggagagca ggaggaactc tggaacgcct tcgaggctcc
      121 catcgatttc ctggtgggcg cctcgaacac ggggggaacc cgaatctacc ggaggctggc
      181 cgaagagcgc atgggaactc ttgcggaagc ccatgccggg ctggccgatg aaaaggttct
      241 ctcggccctg gcggaggaga tcaaaaagac ggtccccggc cctctgatcc agagcacgcc
      301 gggcggtgac gacggcacga aggacttcgc ctcccggctt cccgtcttcc gcatgtccgg
      361 caagcgcttc acccctgatg cctacgtgat gaacatgctt acctcccccc gggtggggac
      421 cgacgcaaac ccccgcaacc tccctgaggg aacggacgtc atggccgccc tcgggtcaaa
      481 ggcggccgtc ggcctggcgg cgaaaaacaa cggcgtcaag gggtacgccg aggcgctgaa
      541 gaagctgaaa acctggataa acgagcacct cggcggggag gagacggtct attccctgtg
      601 gataaaggca ttccacgagg ggttccggga ttccggctcg gaccagttct tttaccgctc
      661 tcccgcatgg cagtggaaga agctctccac cttttccgcc tcctgggccg agctgaagca
      721 cgacacggtt ctctacgccg agcagtccgg cgccgagatg ggagacggcg gcgacaccga
      781 agcggggcct tttgcccctc cgcagccgaa gggctacgtg gagcccgatc cccggacctt
      841 cgacgccctt ctgtcggcgg tgacgcggct gaaggccttc atcgccgaat tcggaatgga
      901 gccggaggac gaggaattcg agaaagaggg cattccctac gcctccaggc tggaaaccct
      961 ggcggaactg ctgaccatcg cccgggacat cgcggcaaag gagaccggcg gcacactcct
     1021 gacggcggag gactacggga acatcaagta cctggccagg gccttcaacg cccagctgct
     1081 gctgcccgga gaaatggtgg aggacacgaa gcagctccgc atggccctgg tcaccgatat
     1141 cgccgcggac tatttcgaag gcagggtgct gcacatcggc tccggcaggc cccagagaat
     1201 ccatgttttc gtgcatgacg cctcgggcgg cccccgggtg acgaggggat tcatcttctc
     1261 ctattacgag ttcccccgct ccctgggcga cggccgcatg accgacgaag agtggaaagc
     1321 actggtctac aacgacggcc ggtccgggga actgaagcaa taccatcctg catggtatga
     1381 agagcttcgg aaataagttc tttccggcag tgctgctcct ctgtgtccta agcgcagcgg
     1441 gagccattcc gcagaaggcg gaaggcgggg cggaactccg ctccgccttc ccctcaggga
     1501 aaggcgccgg ggaaagggag gggcctgccc gcatcatcgg cggcatcgtt ccccaccatg
     1561 atcttgccct cggcatgatc cgacggtttt acgagcgcct cggctcgccg gacgtccggc
     1621 gggtctggct gctttccccc gatcacttcc gccgtgcccg gaccttcgcg gccctgtgtc
     1681 ccgacgactg gaagaccccg gaagggatcc tcagggcgga ccgggaagcc tgtgaaacgc
     1741 tcccgtccct ttctgtggcc ggggctgacg gagccctgtt ccggcgggag cacggcataa
     1801 ccatccacat ccctttcatt gcccggcatt ttcccaacgc gtcggtggtt cccctggtga
     1861 tcggcgcgcg gacgcccgac ctggcgctgg tcatcctgaa gaacgccgtc agagatctgc
     1921 tccgtgacgg cgatgccgtc atcctgagca tggacctgtc ccattacaag acaccggagg
     1981 ccatggccgc cgaggaccgg aagaccctgg atgtcctggc ggagcttcgg ccg
//
LOCUS       DAOTHD010000396         2031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_65679_length_2031_cov_3.395243, whole genome shotgun sequence.
ACCESSION   DAOTHD010000396 DAOTHD010000000
VERSION     DAOTHD010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65679_length_2031_cov_3.395243"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="P5219_07240"
     CDS             complement(<1..462)
                     /locus_tag="P5219_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26589.1"
                     /translation="MRKSFVPMLYLFLIQVLSIPAFGNGPPHPSPPPDSGHSFFVFLH
                     SVTNPDSIELRMDEPPDREGRIRHASFVIRGACFGGLRVERIAAEFSFLELNAPGEWR
                     PGRPRSLRVKDALRTNAEVVILEKDINDALGSFPSRRGSISIDFFPGSVLVA"
     gene            complement(670..>2031)
                     /locus_tag="P5219_07245"
     CDS             complement(670..>2031)
                     /locus_tag="P5219_07245"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRX26590.1"
                     /translation="SCLRMLGFGRFEDLAGRNMHFLIHYAYRNGRLMELEECKIHATL
                     RTGRGFHVDDEVFWTSGGTPLDVAYSSFPQFRNGEIVGAVITFTDNTERRKSQAHINY
                     LTYHDSLTGLYNRAYFEEELKRLNREENLPLTIIFGDLNGLKLTNDVFGHAAGDELLK
                     KAAEALRKSCREGDIISRVGGDEFTIILPRTTADTAEKIIVRIREEFSKVRVNAIKAS
                     IAVGCAVKAVPGGPAEETVKDAEEAMYKQKSLNRKAVGSEMIGTIMETLHEKSPKEKK
                     HSENMSRMCREIGRSMKMSETEIRKLTEAGYLHDIGKIILGEDILDKGNELQPMDQYL
                     DREMAQHPVAGFRILNLFEDTMDLAEEILSHHENWDGSGYPRGIGGEEIPQMARILRA
                     GEVFDDFMESLEEGRMTGEEVLEQLGKLRGTVLDPAIADILLSMARKTEAGGGTGGLT
                     DIF"
BASE COUNT          375 a          604 c          580 g          472 t
ORIGIN      
        1 ggcgaccagt accgacccgg gaaaaaaatc gatggaaatg ctgccccggc gggatggaaa
       61 ggacccgagg gcatcattga tgtctttctc gaggatcacc acttccgcat tggtccggag
      121 ggcgtccttc accctgaggg aacggggccg gccgggacgc cattccccgg gagcattcag
      181 ctcgaggaag gaaaattcgg cggcgatcct ttccacccgg agtcccccga aacacgctcc
      241 ccggatgaca aaagaggcgt gccgtatccg gccctcccgg tcagggggtt cgtccatgcg
      301 gagttctatc gaatcggggt tcgtaacgga atggagaaag acaaaaaagg aatggccgct
      361 gtccggagga ggggagggat ggggcggacc gttgccgaaa gcgggaatgg agagaacctg
      421 tataaggaaa agatacagca tcggaacgaa gctttttctc atggcccttc ctccgcgggt
      481 gttctgcccg gcgttcgaaa agcggcctgt attcgcggga tgccgcagcc ttcggggcag
      541 gaatagggcg atctcccgat aaagtgtacc ctatctgcaa tcggcagtcc agcgtcagcc
      601 gcgggagagg gtccgggaag aggaggggct ttcccccgga ccgtttcacg gcgccggata
      661 acggcttgct cagaaaatgt ccgtgaggcc gccggtgccg ccccctgctt ccgtctttcg
      721 ggccatggat agaagaatat cggcgattgc cgggtcgaga accgtgcccc tcagctttcc
      781 gagctgttcc aggacttctt ctccggtcat tctgccctct tccagggact ccataaaatc
      841 gtcgaagact tctccggcgc gcagaatcct ggccatctga ggtatttcct ctcccccgat
      901 tcccctgggg tatcccgacc cgtcccagtt ttcgtgatgg ctgaggattt cttccgccag
      961 gtccatggtg tcctcgaaca ggttcaggat cctgaaacct gccaccggat gctgggccat
     1021 ctccctgtcc aggtactgat ccatgggctg gagttcattt cccttgtcca gaatatcctc
     1081 gccgaggatg atcttcccga tatcatgaag ataccccgct tccgtcagct tccgtatttc
     1141 cgtctccgac atcttcatgg agcggcctat ctctctgcac atccggctca tgttctccga
     1201 gtgcttcttt tccttggggc ttttttcgtg aagggtctcc atgatggtac cgatcatctc
     1261 tgaaccgacc gctttgcggt tgaggctctt ctgcttgtac atggcctcct cggcgtcctt
     1321 cacggtctct tcggcggggc cgccgggtac agccttcacc gcgcagccca cggcaatgct
     1381 tgccttgatg gcattgacgc ggaccttcga gaactcttcc ctgatcctga cgatgatttt
     1441 ttcggcagta tctgccgttg tgcggggaag aatgatggtg aactcgtctc ctcccaccct
     1501 ggagatgatg tcgccctccc tgcagctctt tctcagggct tccgccgcct ttttcagcag
     1561 ttcgtccccg gcggcgtggc cgaagacgtc gttcgtgagt ttcaggccgt tcagatcccc
     1621 gaagataatc gtgaggggca ggttttcctc cctgttcagc ctcttcagtt cctcttcgaa
     1681 gtacgccctg ttgtacaggc cggtgaggct gtcgtggtag gtcaggtaat tgatgtgcgc
     1741 ctggcttttc cttcgctcgg tgttgtccgt gaaggtaatc acggcaccga cgatttcccc
     1801 gttccggaac tgggggaacg acgagtatgc cacgtccagg ggcgtaccgc cggatgtcca
     1861 gaaaacctcg tcatccacgt ggaaacctct tccagtccgg agtgtggcgt ggattttaca
     1921 ttcctcgagc tccatgagcc tgccgttcct gtaagcgtaa tggatcagga aatgcatatt
     1981 cctgccggcc agatcctcaa aacgtccgaa cccaagcatg cgcaggcagc t
//
LOCUS       DAOTHD010000397         2029 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_65768_length_2029_cov_2.596251, whole genome shotgun sequence.
ACCESSION   DAOTHD010000397 DAOTHD010000000
VERSION     DAOTHD010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65768_length_2029_cov_2.596251"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            450..>2029
                     /locus_tag="P5219_07250"
     CDS             450..>2029
                     /locus_tag="P5219_07250"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HRX26591.1"
                     /translation="MQLYLEEGIGADLVAKELGLSGATVHDWAKRYKKYGEAGLRHPC
                     RKSGKPKLPLAVKEKIAELKRSDPGQGSRRISQVLKRFFFLKASPETVRRELKASDGV
                     HPKPKARRKPKPPARRFEASVPNQMWQTDITYYPVLGKMAYIIGFIDDHSRYITGLGL
                     YRSQTSENVVETYRMATGAFGIPKEMLTDNGRQYASWRGMTKFQKELKKDHIHHIRSS
                     PHHPMTLGKIERFWQTLKEEYLDRARFETFEEARDRLAYWVKYYNHKRPHQGLDGLVP
                     ADRFFSIQKEMKAAIERGVAANVEEMALRGKPKEPFYLVGRIGDRSVVIETDQKRVSV
                     MLDGQELKAGEAITYERSSHEAGTGGKEAEAEGEGVQREGEDAGGAGAVERAAEHVGS
                     DEGHGSAVGAGEHLGEACAFGDAACAGPETEANRGRSFAASGAAGEADRTGGEAGSAG
                     ERELKGDEHEDTGTGTVQRLGEMPGGAGGLDGEEEGLGAVPGNGSECGVVFAVAGPGA
                     FGHIGGAGAAGNEGRPGEA"
BASE COUNT          464 a          496 c          738 g          331 t
ORIGIN      
        1 gaatgtgtta cctatgtcct gacattatag tgttaccaat gtcctgactg cacccttgtg
       61 gtgaattttc tgaagtctct ggtctctagt ctctggactc tagtcttttt cttcttcctg
      121 caaccttcat ttttctggaa ttctttctgg ctttccgctc cgccccggcg ttattcttcg
      181 gaacggcgag ggggtccagg aggacggact gaaagtgaat gacagaccgt gcgctagcga
      241 ccagataatg agacttaaga tttagtgaaa gagggtaaca ctccgggtga tggagaggag
      301 tgttaccgat gaagaagaga gaagcaggaa gaggaagtcc ccccagaggg acgcccccta
      361 gggggcgcgc gcgaccgcag ccgaaagcgg cccggaattc aaaaccctac cggccggggc
      421 cgtatccata cgagatccgt cggcgggcgg tgcagctgta ccttgaagaa gggatcggcg
      481 cggacctggt ggcgaaggaa ctggggctgt cgggggcaac ggttcacgac tgggcgaagc
      541 gttacaagaa gtatggggag gcgggattgc ggcatccgtg ccgcaagagc gggaagccca
      601 aacttcccct ggcggtcaag gagaagatcg cggagctgaa acgcagtgat cccggccagg
      661 gatccaggcg gatctcgcag gtgctgaagc ggttcttctt tttgaaggcg agcccggaga
      721 cggtgcgccg ggagctgaag gcatccgatg gtgttcatcc gaaacccaaa gcccgccgca
      781 agcccaagcc gcccgcgaga cgcttcgagg cgtcggtgcc gaaccagatg tggcagacgg
      841 acatcaccta ctacccggtt ctgggcaaga tggcctacat catcgggttc atcgatgacc
      901 attcccgcta catcacgggg ctggggctgt accggagcca gaccagcgag aacgtggtgg
      961 agacctaccg catggcgacg ggggcgttcg gcatcccgaa ggagatgctg acggacaacg
     1021 ggcggcagta cgcaagctgg cggggcatga cgaagttcca gaaggagctg aagaaggacc
     1081 acatccatca catccgcagt tccccgcatc atccgatgac gctggggaag atcgagcggt
     1141 tctggcagac gctgaaggag gagtatctgg accgggcgcg gttcgagacc tttgaggagg
     1201 cgcgggatcg tctggcgtac tgggtgaaat actacaacca caagcggccg caccagggac
     1261 tggacgggct ggttccggcg gaccggttct tctcgatcca gaaggagatg aaagcggcga
     1321 tcgagcgcgg ggtggcggcg aacgtggaag agatggcgct tcggggcaaa ccgaaggagc
     1381 cgttctacct ggtgggccgg atcggggacc ggagcgtggt gatcgagacg gaccagaagc
     1441 gggtcagcgt gatgctggac gggcaggaac tgaaggccgg agaggccata acgtacgaga
     1501 ggagcagtca tgaagcagga acaggcggaa aagaagcaga agcggaaggc gaaggtgtac
     1561 agcgcgaagg agaagacgca ggcggtgctg gcgctgtgga gcgggcggcg gagcatgtcg
     1621 gatctgatga aggccatgga agtgccgtgg gggctggtga gcacctggga gaagcgtgcg
     1681 ctttcgggga tgctgcatgc gctggacccg aaacggaagc aaacagagga aggtcctttg
     1741 cagcttccgg tgcggctgga gaagctgatc ggacaggcgg tgaagccgga agtgccggag
     1801 aacgcgaact gaagggagat gaacatgaag acacaggaac aggaacggta caacgcctcg
     1861 gagaaatgcc gggcggtgct ggcggtctgg acggagagga agaaggcctc ggagctgtgc
     1921 cgggaaatgg gagtgagtgc ggcgttgttt tcgcagtggc aggaccgggc gctttcgggc
     1981 atattggagg cgctggagcc gcggggaacg aaggacggcc cggagaagc
//
LOCUS       DAOTHD010000398         2019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_66219_length_2019_cov_3.028513, whole genome shotgun sequence.
ACCESSION   DAOTHD010000398 DAOTHD010000000
VERSION     DAOTHD010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66219_length_2019_cov_3.028513"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..1508)
                     /locus_tag="P5219_07255"
     CDS             complement(129..1508)
                     /locus_tag="P5219_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HRX26592.1"
                     /translation="MAVTEYVGVEKITGPFLLLSGIPGVGYDEIVDVLSPDGGVRKGR
                     VVLVDRNSTLVQVFSGTEGLAPASTAVRFLGRELEISLSPSLLGRTFSGIGEPRDGCG
                     PVLGGVRRAVNGSAINPMAREYPRDFISTGISSIDVLLTLIRGQKLPIFSGNGLPHNQ
                     LAVQIATQSRLVGAENFAVVFAGIGIKHDDAAYFQQRLSAHGQGGHLVLFLNLADDPV
                     IERIATPRFALTAAEYLAFDLGMHVLVIMTDMTSYCEALRELSVARGEVPSRKGYPSY
                     LYSDLASLYERAGVLRNGRGSVTQIPILTMPNDDITHPIPDLTGFITEGQVVLSRDLE
                     ARGIYPPVEVLPSLSRLMKDGIGKGYTREDHPDLAGQLFASYSRVHDVRSLAGVVGED
                     ELSPADRASLSFGDAFERSFLSQGGEENREISQSLDLGWELLSSLPVQELSRLSMEEI
                     EKYIRKKGA"
     gene            complement(1512..>2019)
                     /locus_tag="P5219_07260"
     CDS             complement(1512..>2019)
                     /locus_tag="P5219_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRX26593.1"
                     /translation="FPAISWIDSYSEYGDEVKDWYAANVNPAWASLRERAREILAEDD
                     KIQQIIRLVGEDVLPDDQKLTALTASLVKNGYLQQGAFGPDSYCPPEKGLAILDLLLG
                     FHEKAERLLQRGCPFSLLRGMKELVELTHLREIPAEKKEAFADLKKRLFERMETVDRE
                     RIAPGREG"
BASE COUNT          342 a          645 c          664 g          368 t
ORIGIN      
        1 cgctcaggat gacagcctga tcccgaggtc gtaaaaccag attcctcggg cttccccctt
       61 cggaatgaca aacgacagga agggccgggg aaatttcccc ggcccttcct gtcgttttcc
      121 gggtactgct acgcgccctt tttccggatg tacttttcga tctcctccat ggagagccgg
      181 gacagttcct gcacgggcag ggaggagaga agctcccatc ccaggtcgag ggactggctg
      241 atctcccggt tttcctcccc tccctggctg aggaaggacc gttcgaaggc atcgccgaag
      301 gacagggacg cccggtcggc cgggctcagc tcgtcctcgc ccaccactcc ggcgagggac
      361 cgcacgtcgt ggaccctgct gtaggacgcg aagagctgcc ccgccagatc ggggtgatcc
      421 tccctggtgt agcccttgcc gatgccgtcc ttcatgagcc gggagaggct tggcagcacc
      481 tccacgggag gatagatgcc ccgggcctcc aggtccctgg agaggacgac ctgtccctcg
      541 gtgatgaatc ccgtgaggtc cggaatgggg tgggtgatgt cgtcgttggg catggtgagt
      601 atgggaatct gggtaacgct cccccggccg ttccgcagca ccccggcccg ttcgtagagg
      661 gacgccaggt cgctgtacag gtaggagggg taccccttcc ggctcggtac ctcgccccgg
      721 gccacggaga gttcccggag agcctcgcag tagcttgtca tgtcggtcat gatcaccagc
      781 acgtgcattc caaggtcgaa ggcgaggtat tccgctgccg tgagggcgaa cctcggcgtg
      841 gcgatccgct cgatcaccgg gtcgtccgcc aggttgagga agagcacaag gtgccctccc
      901 tgcccgtggg cggagagccg ctgctggaaa taggcggcgt cgtcgtgctt gatgccgatg
      961 ccggcgaaga ccacggcgaa gttctcggcc cccacgagcc gggactgggt ggcgatctgc
     1021 accgccagct ggttgtgggg caggccgttc cccgagaaga tggggagttt ctgtcccctg
     1081 atcagggtca ggaggacgtc gatggacgat atgcccgtgg agatgaagtc ccggggatac
     1141 tccctggcca tggggttgat ggccgacccg ttcacggccc tgcggacgcc gccgagcacc
     1201 gggccgcacc cgtccctggg ctcgccgatg ccggagaagg tccggccgag gagcgacggt
     1261 gacagggaaa tctcaagctc ccggccgagg aagcggaccg ccgtggaggc aggggcaaga
     1321 ccctccgtac cggagaagac ctgcaccagg gtggaattcc ggtccaccag gaccacccgg
     1381 cccttccgga cgccaccgtc aggggagagc acgtccacga tttcgtcgta tcccacaccg
     1441 gggatgcccg acaggagcag gaaggggccg gtgatcttct ccacgcccac gtattctgtg
     1501 acagccacgg cttatccctc ccttccgggg gcgatgcgct cccggtcaac cgtctccatg
     1561 cgctcgaaga ggcgtttttt cagatcggcg aaggcttcct ttttctctgc cggaatttcc
     1621 cttaaatggg tcagctccac gagctccttc atcccccgga gcagcgagaa ggggcatccc
     1681 ctctggagaa gtctttccgc cttctcgtgg aagccgagga gaaggtcaag gatggccagc
     1741 cccttctccg gcgggcagta ggaatcgggt ccgaaagccc cctgctgcag gtagccgttc
     1801 ttcaccagcg aagcggtgag ggctgtaagc ttctggtcgt cggggaggac gtcctcgccc
     1861 acgaggcgga tgatctgctg gatcttgtcg tcctccgcca ggatctcccg ggcccgctcc
     1921 ctcagggacg cccatgccgg gttcacgttg gcggcgtacc agtccttcac ctcgtcgccg
     1981 tactcgctgt acgagtcgat ccagctgatg gcggggaag
//
LOCUS       DAOTHD010000399         2018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_66256_length_2018_cov_3.239429, whole genome shotgun sequence.
ACCESSION   DAOTHD010000399 DAOTHD010000000
VERSION     DAOTHD010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66256_length_2018_cov_3.239429"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="P5219_07265"
     CDS             complement(<1..1162)
                     /locus_tag="P5219_07265"
                     /inference="COORDINATES: protein motif:HMM:NF016203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HRX26594.1"
                     /translation="MAERDKRDIRCIVLKPGDSVAVLPEGGKAGECLSGTGVVLREDI
                     PAGHKAALRSVLRGEPVIKYGHPMGRATMNIVPGEKVHVHNVESALRAEWDMTWTPSG
                     AAFREPSVPVPVFAGYAREQGAPGIRNELWIIPLVGCVNDYLKFLADGYSPPSWIERI
                     RVLSHPYGCSQLGDDLDRTAAVLEGLARNPCAAGVVVAGLGCENLRRVFMETRLSDLR
                     KVRFFSLQEAEDDRRLLYGLLDGLAEAAPRARTEFPLSDVTIGVKCGGSDGFSGLSAN
                     PLAGEVADMMCAWGGRVVATEIPEMFGAEDVLASRIADGEVFRAFAGTIRWFRDYYDA
                     HNQPVYENPSPGNKDGGITTLEEKSLGAVNKCGSSPVTDVLTMGAQAVRRGVS"
     gene            complement(1295..>2018)
                     /locus_tag="P5219_07270"
     CDS             complement(1295..>2018)
                     /locus_tag="P5219_07270"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRX26595.1"
                     /translation="LLSWFLRGKTIVRLGPAGIAGSPGTYLRILKNGLPSFCRQALAS
                     IATIALNVSAAVYGDAAVAAMSIVGRLFMFVFAVNLGIGQGYMPVAGYNYGAGRFDRV
                     RKAFFFTLKVTAVLMTISGIAGFAAAPFLIRLFIRNDADVVRIGVGALRAMCLAMPLV
                     PLGVVCNMTFQSIGRSWAATVLSLARQGIFFLPLILTLPGFIGLKGVQITQPLADALT
                     FLSCLPLTYAFFRGLEPETRTG"
BASE COUNT          408 a          645 c          626 g          339 t
ORIGIN      
        1 ccgagacgcc ccggcggact gcctgggcgc ccatggtgag cacgtcggtc acgggagagg
       61 atccgcactt gttcaccgct cccagggact tttcctccag ggtggtgatg ccgccgtcct
      121 tgtttcccgg agaggggttt tcgtacaccg gctggttgtg ggcgtcgtaa tagtcccgga
      181 accacctgat ggtgccggcg aaggccctga agacctcccc gtcggcgatc cgggaggcga
      241 gcacatcctc ggcgccgaac atttccggaa tttccgtggc cacgaccctg ccgccccatg
      301 cacacatcat gtcggcgact tcccccgcca ggggattggc cgagagcccc gaaaacccgt
      361 cgctgccccc gcacttcacc ccgatggtca cgtcggaaag gggaaattcc gtccgggccc
      421 ttggcgccgc ctccgccagg ccgtcgagga gtccataaag gaggcgccgg tcgtcctccg
      481 cctcctggag ggagaaaaac cggaccttcc ggaggtccga gagcctggtc tccatgaaga
      541 ccctccggag attctcgcac ccgagccccg ccacgacgac gcccgccgcg cagggatttc
      601 tcgcgaggcc ctccaggacc gcagcggtcc tgtcaaggtc gtcgccgagc tgagaacacc
      661 cgtaggggtg ggagagaacc cggatccgtt ctatccatga agggggggaa tagccgtcgg
      721 cgaggaattt gaggtagtca ttgacgcatc ccacaagggg aatgatccag agttcgttcc
      781 gtatgcccgg agctccctgc tcccttgcgt accccgcaaa caccggaacg ggcaccgaag
      841 gttcccggaa ggccgcgccg gaaggtgtcc aggtcatgtc ccattccgcc cggagagcgg
      901 actccacgtt atgaacatga accttttccc ccggaacgat attcatggtg gcccggccca
      961 tgggatggcc gtacttgatc accggttccc cccggagaac gctccgaagg gcggccttgt
     1021 gacccgccgg aatatcttcc cgaagcacca ctccggttcc tgaaaggcat tctcccgcct
     1081 ttccaccctc cgggagcacc gccacggaat ccccgggctt cagaactata cagcggatgt
     1141 ctcttttatc ccgttctgcc acaacggacc cctccctttt tgttccacgg cgggttgcct
     1201 tccgcccaat aaacgttttc ccaaacatag cggagaacgg ggaaaagtca agaccggact
     1261 tccagccccc tccggaaaca gggacgggaa accgtcagcc ggtacgggtt tcaggttcga
     1321 gccccctgaa aaaagcatac gtgaggggaa ggcagctcag aaacgtcagt gcatcggcca
     1381 ggggctgggt gatctgcacc cctttcagtc cgatgaatcc cgggagcgtc agaatcaggg
     1441 ggagaaaaaa gattccctgc cgggcgaggg acagcaccgt ggctgcccac gaccttccga
     1501 tggactgaaa ggtcatgttg cagacgacac ccagggggac caggggcatg gcgaggcaca
     1561 tggcccggag ggcgccgacg cctatgcgca ccacgtcggc gtcgttcctg ataaagagcc
     1621 tgatcaggaa cggggctgcc gcgaagccgg cgatcccgga aatcgtcatc aggacggccg
     1681 tgactttcag ggtgaaaaag aacgctttcc ggaccctgtc aaaacgtccc gcaccgtagt
     1741 tgtacccggc gacaggcatg tatccctgcc cgatgccgag gttgacggca aagacaaaca
     1801 tgaagagcct gccgacgatg gacatggcgg ccacggcggc atcgccgtag acggcggcgc
     1861 tgacgttgag ggcaatggtg gcgatgctcg cgagggcctg gcggcagaag gagggaagtc
     1921 cgttcttgag aatcctgagg taggttccgg gactgcccgc gattcctgcc gggcccaggc
     1981 ggacgatggt ctttccccga agaaaccatg agaggaga
//
LOCUS       DAOTHD010000400         2014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_66458_length_2014_cov_2.644717, whole genome shotgun sequence.
ACCESSION   DAOTHD010000400 DAOTHD010000000
VERSION     DAOTHD010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66458_length_2014_cov_2.644717"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..1322)
                     /locus_tag="P5219_07275"
     CDS             complement(75..1322)
                     /locus_tag="P5219_07275"
                     /inference="COORDINATES: protein motif:HMM:NF016252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HRX26596.1"
                     /translation="MFPCAQHRRSYRLIAVAALLAAAFCLLPARSRASEPPSAPAEFH
                     AFSFVDVKHKAEELASRPFEDPEGQVPDFLLNISYDQWRKIRFRPSESLWRKEGLPFE
                     VQFFHPGLFYNRLVAVNVVDGGRAEKLTFSPSVFEYGDEEFASKVAGTPLDFAGFRIH
                     YNLNTGKYKDEVAVFLGASYFRAVARDGQYGLSARGLAVDTALQSGEEFPYFREFWIE
                     KPREDSDSITVHALLDSPSLAGAYTFTIIPGKETSMDTECVLFLRKDVQKLGIAPLTS
                     MFLYGETENGRPGDYRPEVHDSDGLLIRTNSGEWIWRPLANPGRLALIPFPQENPAGF
                     GLMQRDWIFDHYQDLEARYEKRPSLWIEPRGNWGRGWVELVEIPSELEIHDNMVAYWV
                     PGKGAEKGPDGQPDGEFLDAALD"
     gene            complement(1428..>2014)
                     /locus_tag="P5219_07280"
     CDS             complement(1428..>2014)
                     /locus_tag="P5219_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRX26597.1"
                     /translation="GAFALESACRQSVEWSGMGLDIPVAVNISAKQISSEFFVRDVLE
                     TVGRTGANPFLLHLEITESAFMENPDHAQKVMAELGEAGIRFSLDDFGTGFSSLAKLR
                     LLPISELKIDRSFILDLESDKTRGVVSTMIHLAGALHLQVTFEGVETKAQMEILGSLV
                     PEGMETAIQGYIFSRPLPPDRVPQFFASELPDVF"
BASE COUNT          403 a          579 c          649 g          383 t
ORIGIN      
        1 gcgtcctcgc cagatctaca ccggcgaacg gcagcgcgac gtggtgccgc tgcacgagcg
       61 gggctgatcc gggctcagtc gagcgcggcg tccaggaatt ccccgtcggg ctgaccgtcg
      121 ggcccctttt ccgccccttt cccgggaacc cagtaggcca ccatgttgtc gtggatctca
      181 agttccgatg gaatttccac aagttccacc catccccgcc cccagttccc ccggggctcg
      241 atccagagag aggggcgctt ttcgtaccgc gcttccaggt cctggtaatg atcgaagatc
      301 caatcccgct gcatcaggcc gaatccggcg gggttttcct gcgggaaggg aatgagagcc
      361 agcctgccgg ggttggccag gggacgccag atccactctc ctgaatttgt ccggatcagg
      421 aggccgtcgg aatcgtggac ctcgggacga tagtcgccgg gcctgccgtt ttccgtctcc
      481 ccgtagagaa acatgctcgt caggggggcg atgccgagct tctggacgtc tttcctcaga
      541 aaaagaacgc actccgtgtc catggaggtc tcctttccgg ggatgatggt gaaggtgtag
      601 gctcccgcaa ggctcgggct gtccaggaga gcgtgaacgg tgatggaatc gctgtcctcc
      661 cggggttttt caatccagaa ttcccgaaaa taggggaatt cctcccccga ctggagagcc
      721 gtgtccactg cgaggccccg ggcggaaagg ccgtactgcc cgtcccgggc cacggcacgg
      781 aagtagctgg ccccgaggaa gacggcaacc tcgtccttgt attttcccgt gttcaggttg
      841 tagtggatcc ggaagccggc gaaatcgagg ggcgttccgg cgaccttcga ggcgaattct
      901 tcatcaccgt attcgaagac ggacggggag aaggtgagtt tctccgcgcg gccgccgtcc
      961 acgacgttga cggccaccag gcggttgtag aaaaggcccg gatggaagaa ctgcacctcg
     1021 aaaggcagcc cctcttttct ccacagggat tcggagggac ggaacctgat tttcctccac
     1081 tggtcgtagc tgatgttcag cagaaaatcg ggaacctgcc cttccgggtc ctcgaaggga
     1141 cgggatgcca gttcttcagc cttgtgcttc acgtcaacga aggaaaaggc atggaattcc
     1201 gccggagcgg aagggggttc cgacgcacgg gacctggcgg gaagcaagca aaacgccgcc
     1261 gcgaggagcg cggcgacggc aatcagacgg tatgagcggc ggtgctgggc gcagggaaac
     1321 acgaaggacc ctccttggcc ggattttccg gaccggttgt gaaatcagat acatcataat
     1381 aagccttttt tgccggagtg caagagggga gggaaatttc tctaatttca gaaaacgtcg
     1441 ggaagttcag aggcgaagaa ctggggaacc cggtccggcg gcaggggacg ggagaagata
     1501 tacccctgga tcgcggtttc catgccttcg gggacgaggc tcccgaggat ttccatctgg
     1561 gctttcgtct cgacgccttc gaaggtcacc tgcagatgaa gggccccggc gaggtggatc
     1621 atggtggaga caacgcccct ggtcttgtcg ctttcgaggt ccagaatgaa ggacctgtcg
     1681 atcttcagct ccgagatcgg cagcaggcgg agcttcgcga gagaggagaa gccggtgccg
     1741 aagtcgtcaa gggaaaagcg gattcccgcc tctccgagct cggccatgac cttctgggca
     1801 tgatccggat tttccatgaa ggcggactcc gtgatctcca gatggaggag aaagggattc
     1861 gcgccggtgc gcccgacggt ttcaaggacg tccctgacga agaattccga ggagatctgc
     1921 ttcgccgaga tgtttaccgc caccggtatg tccagtccca ttcccgacca ttccacggac
     1981 tggcggcatg cgctctcgag ggcgaaggcg ccga
//
LOCUS       DAOTHD010000401         2010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_66625_length_2010_cov_3.682353, whole genome shotgun sequence.
ACCESSION   DAOTHD010000401 DAOTHD010000000
VERSION     DAOTHD010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66625_length_2010_cov_3.682353"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1691
                     /gene="dxs"
                     /locus_tag="P5219_07285"
     CDS             <1..1691
                     /gene="dxs"
                     /locus_tag="P5219_07285"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HRX26598.1"
                     /translation="HQAYAYKLLTGRLDRFDTLRQWGGVSGFPRRRESPFDHFDAGHS
                     STSLSAALGFAKARDVLGQKHHVVAVIGDASLLNGLAFEALNYTRESRTKVIYVLNDN
                     TMSISPRVGGMATHLARLSSSSAYNWLKKAIKDSCASLPRGQALENVLGKMKDHIKTI
                     VKPVNIFDEMDINYWGPFDGHNTEELENVFELAKKYDRPVLLHVVTVKGKGSEQAEKD
                     PTAFHGVSPARPKECCAAVGRSWSEAAAGIVLDMAENDSRIVCLTAAMKCGSRLDEFA
                     SRFPDRFFDVGIAEGHMVTMAAGMAAGGLKPVVFVYSTFLQRAMDQVVHDVCMQNLPV
                     VFAVDRAGLVGEDGETHQGLLDIPWSRPVPNLVLTAPRDEEALRRLFLFAGKHDGPMM
                     IRFARGTAVPCLKKGNSLLAPSAPDILKPEILEKGSEWALIGYGKTVELMLRARETAR
                     SLGISPLPTVMDLRCLKPLPEQELRELLTTHSFTVIAEDGYVAGGAGEAIAAMVRNMT
                     EERPAEISLMGVPDIFVPQGRIREQEEHCGLTPGKVVNCFVEFQKRAYRQAARI"
     gene            1645..>2010
                     /locus_tag="P5219_07290"
     CDS             1645..>2010
                     /locus_tag="P5219_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRX26599.1"
                     /translation="MSSSKKERIDRLLVSRGLVETRSKAQALLLAGKVFVGGSRVDKT
                     GSLIPADAPVEVRDDGEKWVSRGAHKLLKGLDFFAISPGGLVCVDIGASTGGFTQVLL
                     ERGARKVYAVDVGYGQLAWS"
BASE COUNT          437 a          550 c          615 g          408 t
ORIGIN      
        1 ggcaccaggc ctacgcatac aagctcctca ccggaaggct tgaccggttt gacactctca
       61 ggcagtgggg aggggtcagc ggttttccga gaaggaggga aagtcccttc gaccattttg
      121 acgccggaca cagcagcacc tctctctcag ccgcccttgg gtttgccaag gcgagggatg
      181 ttcttggaca aaagcaccac gtggtagccg taatcggcga tgcctccctg ctcaacggcc
      241 tcgccttcga ggcgctgaac tatactcggg aatcacggac gaaagtgatt tatgttctca
      301 acgacaacac catgtccatc agccccaggg tgggaggcat ggcaacccac ctggcccgac
      361 tgagctccag ctccgcttac aactggctca agaaggccat caaggattcc tgcgcgtctc
      421 ttccccgggg gcaggctctt gagaatgtcc tgggaaagat gaaagaccat atcaaaacca
      481 tcgtcaagcc cgtcaacatt ttcgacgaga tggacataaa ctactggggc ccctttgacg
      541 gccacaatac ggaagaactt gagaacgtct tcgagctggc aaaaaaatac gaccggccgg
      601 tgcttctcca cgtggtgact gtcaagggaa aagggtctga acaggctgag aaggatccta
      661 ccgctttcca cggcgtttct cccgcccggc cgaaagaatg ctgtgcagcc gtgggaaggt
      721 cctggagcga ggcagccgca ggcatcgtgc tcgacatggc cgaaaatgac agccggattg
      781 tctgccttac tgcggccatg aaatgcggca gccggctgga cgagtttgcc tcccggttcc
      841 ctgacagatt tttcgacgtg gggatcgccg aaggccatat ggtgaccatg gcggcgggaa
      901 tggccgcagg agggctgaag ccggtggttt tcgtctattc aaccttcctt cagcgggcca
      961 tggaccaggt ggtgcatgat gtctgcatgc agaatcttcc cgttgtcttc gccgtggaca
     1021 gggccggcct tgtcggggag gacggagaga cccaccaggg gcttttggac atcccttggt
     1081 ccagacctgt gccgaacctt gtcctgactg ctccccggga tgaggaggcc cttcggcgcc
     1141 tcttcctttt cgcggggaaa catgacggac caatgatgat ccgctttgcc agggggacgg
     1201 cagttccctg tctgaaaaag ggaaactccc tgcttgcccc ttcagctcct gatattctga
     1261 agccggaaat actggaaaag ggaagtgaat gggctctcat cggatacgga aaaacagtgg
     1321 agctcatgct cagagcgaga gaaaccgcca ggagccttgg aatttccccc cttcccacgg
     1381 taatggatct caggtgcctg aaaccccttc ccgaacagga actgcgggaa ctcctcacga
     1441 cccactcctt cacggtcatt gcggaggacg gatatgttgc aggcggcgcc ggagaggcca
     1501 tagccgccat ggtgcggaat atgacggagg aaagacccgc ggagatttcc ctgatggggg
     1561 tccccgacat cttcgtccct cagggaagaa tccgggaaca ggaggagcat tgcggcctca
     1621 ctccggggaa agtggtgaac tgttttgtcg agttccaaaa aagagcgtat cgacaggctg
     1681 ctcgtatcta ggggactcgt tgaaacgaga tcgaaagccc aggctctcct tctggcgggg
     1741 aaggtcttcg tgggcggttc ccgggtggac aagacgggtt ccctgattcc cgcggatgcc
     1801 ccggtcgaag tccgggacga cggggaaaaa tgggtcagtc ggggagctca caaacttctg
     1861 aaggggctcg atttttttgc catatccccg ggaggtctgg tttgtgtgga tatcggggct
     1921 tctaccggag gattcaccca ggtgcttctt gaaagagggg cccggaaagt atatgcggtg
     1981 gacgtgggat atggtcagct tgcgtggtcc
//
LOCUS       DAOTHD010000402         1998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_67136_length_1998_cov_3.464745, whole genome shotgun sequence.
ACCESSION   DAOTHD010000402 DAOTHD010000000
VERSION     DAOTHD010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67136_length_1998_cov_3.464745"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /gene="pcp"
                     /locus_tag="P5219_07295"
     CDS             <1..651
                     /gene="pcp"
                     /locus_tag="P5219_07295"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: protein motif:HMM:NF009676.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HRX26600.1"
                     /translation="DSIMKVLVTGFDPFGGEPVNPAFEAVKRLPRTIAGAHVDILEIP
                     TVFHKSIEAIVKRAEEMCADRILMVGQAGGRFEITVERVGINVDDARIPDNEGNRPID
                     VPIDPDGPAAHFATLPVKAMTERIRSRGIPAKVSNTAGTFVCNHVLYGVLNAVAKKGL
                     PVKAGFIHVPYLPEQAAGKDSVPSMSAATIAAALEAAVEAMVTTGEDVKISGGREY"
     gene            858..1721
                     /locus_tag="P5219_07300"
     CDS             858..1721
                     /locus_tag="P5219_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRX26601.1"
                     /translation="MQLFFEQLLNGVAMGAIYSLITLGLALVYGVMRILHVAHAAVYT
                     AGAYVGLYVFTMTGSLLLAVPAAMAACALLGTAIERLIYTPLLKYPPFVPLIGSIAVF
                     LALEEIFRLVGGPYILSFPAEFPFPGITVRGVVISPAIISIYAVSAAVLLLLWFVVTK
                     TELGLAMRATSQDREIAGAMGINSSFTISMTFVLGSAVAAVAGILVGIYFNQVYPTMG
                     AVPAYKCLALIVVGGLGSLPGAVLASLLLGVGETLLIGYANIPLPRDSLAFIAMIAVL
                     LWRPQGLLGNR"
     gene            1735..>1998
                     /locus_tag="P5219_07305"
     CDS             1735..>1998
                     /locus_tag="P5219_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRX26602.1"
                     /translation="MNDYSITILTFIAIQAIVACGLNVIVGYAGQISLGHAAFFGIGA
                     YASALLTTKAGFTFWTALPLVILLTGAIGLVLGMPSLRLREDFL"
BASE COUNT          331 a          666 c          596 g          405 t
ORIGIN      
        1 gattccatca tgaaagtgct tgtcacgggc ttcgacccct tcgggggcga gccggtcaat
       61 cccgccttcg aggcggtgaa gcgtctgccc cggacaatcg ccggtgctca tgtagacatc
      121 ctggagattc ccacggtgtt ccacaagtcc attgaggcga tcgtgaagag ggcagaggaa
      181 atgtgcgccg accggatcct catggtcggc caggccggag ggcgcttcga gatcaccgtg
      241 gagcgggtgg gcatcaacgt ggacgacgcc aggataccgg acaacgaggg caaccgcccc
      301 atcgacgtgc ccatagaccc cgacgggcct gcggcccatt tcgccaccct tccggtgaag
      361 gccatgacgg agcggatccg ctcccggggc attcccgcga aagtgtcaaa cacggcgggg
      421 accttcgtgt gcaaccatgt cctctacggc gtgctgaacg ccgtcgcgaa gaaaggactt
      481 cccgtgaagg ccggctttat ccacgttccc tacctcccgg agcaggcggc gggaaaggac
      541 agcgtcccgt ccatgtccgc cgccaccatt gccgctgccc tcgaggccgc cgtggaagcc
      601 atggtgacca ccggagagga cgtaaagatc tccggcggaa gggagtactg agcatggaaa
      661 aatgcacagc ctgaattgtc ggctgtttcc gggagaggaa gtgttcacgc tgcctgcgga
      721 ttcaggtcgt gttccaccgt tttcctccgg gccgggaacg tgttagaata acggcgccgg
      781 atcagaagcc gaaacagacc aataccggtt ttattttttt gccgccattc ctgaaatgcg
      841 gggaggtttt accggccatg cagcttttct ttgagcagtt gctcaacggt gtcgccatgg
      901 gggcaattta ttccctcatc accctcggtc tcgccctggt ctacggcgtc atgcgcatcc
      961 tccacgtggc ccatgcggcc gtgtacactg cgggcgccta cgtgggactg tatgtcttca
     1021 cgatgacggg cagccttctc cttgccgtac ctgccgcaat ggcggcctgc gctcttctgg
     1081 ggaccgccat cgaacggctg atctacactc ccctgctgaa gtacccgccc ttcgtgcccc
     1141 tcatcgggag catcgccgtc ttcctggccc tggaggagat cttccgtctg gtcggcggcc
     1201 cctacatcct ctcctttccc gccgagtttc ccttccccgg aatcaccgtc cggggagtgg
     1261 tcatctcccc ggccatcatc tccatctatg ccgtcagcgc ggcggtgctg ctgctgctct
     1321 ggttcgtcgt gacgaagacg gagctcggcc tcgccatgcg ggccacgtcc caggaccggg
     1381 aaatagcggg agcaatgggc atcaacagct ccttcaccat cagcatgacc ttcgtcctcg
     1441 gctcggcggt ggcggctgtg gccggaatcc tcgtggggat ttacttcaac caggtctatc
     1501 ccaccatggg agccgttccc gcctacaagt gcctcgccct catcgtggtg gggggcctcg
     1561 ggtccctgcc cggcgcagtg cttgcctcgc tgcttctcgg ggtgggagag acgctgctca
     1621 tcgggtacgc caacatcccc cttccgaggg attccctggc cttcatcgcc atgatcgccg
     1681 tgctcctctg gcggccccag ggactcctgg gcaacaggta gggagggaag gaccatgaac
     1741 gactattcca tcaccatcct gaccttcatc gccatccagg ccatcgtggc ctgcggcctc
     1801 aacgttatcg tggggtacgc cgggcagatt tccctcggcc acgccgcctt tttcggcatc
     1861 ggggcctacg cctccgccct gctcaccacg aaggccggct tcaccttctg gacggccctg
     1921 ccgctggtga tccttctcac aggcgccatc gggctggtgc tcggcatgcc gagcctccgg
     1981 ctccgggagg acttcctg
//
LOCUS       DAOTHD010000403         1996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_67224_length_1996_cov_3.609480, whole genome shotgun sequence.
ACCESSION   DAOTHD010000403 DAOTHD010000000
VERSION     DAOTHD010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67224_length_1996_cov_3.609480"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..926
                     /gene="kdsB"
                     /locus_tag="P5219_07310"
     CDS             192..926
                     /gene="kdsB"
                     /locus_tag="P5219_07310"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRX26603.1"
                     /translation="MKTLAVIPARFASTRLPGKPLLPVKGVPLVVRVLRRVETCSSVD
                     RIIVAADDDRIAEAVRHAGGECMLTPPELPSGGDRVAWVAGKIPSELVLNVQVDDPLV
                     GPDMIDPLTAAMDDPSVMLALLSKRIERDEEVSSPNIVKMVFDTAGRALYFSRSPIPF
                     PRSGGPVYYKHIGPYCWRRDFLLSFSSWEQTPLEKTESLEMLRVLERGHSIACVPVER
                     DTIEIDTPEDAEALERYLELHPDCVF"
     gene            complement(991..>1996)
                     /locus_tag="P5219_07315"
     CDS             complement(991..>1996)
                     /locus_tag="P5219_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRX26604.1"
                     /translation="AHAARDHTYLATLHARLQPVADGVLDQRLQQQRRHGQIAQLLRR
                     RGYDPDDQSLDPASAERLISERTRAIMPVHMFGAAADMDRFAELGKKYGIPVIEDACE
                     VVGGTYKGRMLGSLGTCGTWSFDPNKTLTVGEGGIVFTDSEEIHSRMDCYHDHGHIHS
                     KEHDRGAEGKSGLGVNFRLSELQGALGLVALQKMDYALGLLRSAKKRILEAVSDTGIV
                     PRPTHNDEGDTASHVIFMLPSAESAVKFRDAAVSAGGGCSIIADNTWHYAKHWKALES
                     MGEKDWFGARTPSYAPASMAQSDGLLARAAMFGLNIIMDDSSVEKMIRAIRAGAGAAL
                     "
BASE COUNT          385 a          621 c          568 g          422 t
ORIGIN      
        1 gccctcgagg ccatggaagg gtccgccgga acgcagattc tcaaggactg acctcagtct
       61 ctttttcccc aggcatatac agaaagcggc ccctgatggg gccgcttttt tcctgccctc
      121 ccccttccgg tgaaggagag aagcgtgtat catcatgagc cggtatcatt cttccaaaaa
      181 ggagatggat catgaaaacc ctcgccgtca ttcccgccag attcgcaagc acccgcctcc
      241 ccggaaaacc gcttctcccg gtgaaaggag ttcccctggt cgtccgggta ctccggcggg
      301 tggaaacgtg ctcctcagtg gacaggatca tcgtcgcggc agacgatgac cggattgcgg
      361 aagcagtgcg gcatgccggc ggagagtgca tgctcacacc tcctgaactg ccgagcggag
      421 gcgacagggt ggcctgggtc gccgggaaaa taccttccga actcgtcctt aatgtccagg
      481 tcgacgatcc tctcgttgga ccggacatga tcgatcccct cactgcggcc atggatgacc
      541 cctccgtcat gctcgccctc ctgtcgaagc ggatcgaacg ggacgaggaa gtctcctcgc
      601 cgaacatcgt caaaatggtg ttcgataccg caggcagggc tctctacttc agccggtccc
      661 ccattccctt tccaagaagc ggaggccctg tatattacaa gcatatcggc ccctattgct
      721 ggcggaggga tttcctcctt tcattctctt cctgggaaca gacacccctc gaaaaaacgg
      781 aaagcctgga aatgctccgg gtacttgaaa ggggccattc catcgcatgc gttcccgtgg
      841 agcgtgatac catcgaaatt gataccccgg aagatgccga agccctggag cgatacctgg
      901 aacttcaccc cgactgcgtt ttttagtttc ctaaaaaaaa gggccccgga atattccggg
      961 gcctgttttt ctttctccga aggtgagatt tcagagagcg gccccggccc ccgcccgaat
     1021 agcccggatc attttctcaa cagaagagtc gtccatgatg atattgaggc cgaacatggc
     1081 ggccctggcc agcagcccgt cactttgggc catggacgcg ggagcatagg atggtgttct
     1141 tgccccgaac cagtccttct ctcccattga ttcgagggcc ttccagtgtt tggcgtaatg
     1201 ccacgtgttg tcagcgatga tggagcaccc gccccccgct gaaacggcgg catccctgaa
     1261 cttcacggcc gactccgccg aaggcagcat gaaaatcaca tgggaggccg tgtctccctc
     1321 atcgttgtgg gttggccggg ggacaatgcc cgtatcggaa acggcttcca ggatcctctt
     1381 cttggcagac cgcagcagcc cgagggcata gtccattttc tgcagcgcaa caaggccgag
     1441 ggctccctgc agttcgctga gcctgaagtt cactcccagc ccggacttcc cctcggcccc
     1501 acgatcgtgc tctttgctgt gaatatgacc gtggtcatgg tagcagtcca ttctggaatg
     1561 gatttcctcg ctgtcggtga acacgattcc gccttcacca acggtgaggg tcttgttggg
     1621 atcgaagctc caggtgccgc atgttcccag gctgccgagc atccttccct tgtaggtgcc
     1681 gccgacaact tcacaggcat cctctatgac cgggatgccg tattttttcc cgagttcggc
     1741 aaacctgtcc atatctgccg cagctccaaa catgtggacc ggcatgatcg cccgggttct
     1801 ttccgaaata agccgctcag ccgaggccgg gtcgaggctc tggtcgtcgg ggtcgtagcc
     1861 ccggcgtcgc aggagctgcg cgatctgccc gtgccgccgc tgctgctgca gccgctggtc
     1921 gagaacgcca tcggccacgg gctggagccg cgcgtgcagg gtggcgaggt acgtgtgatc
     1981 gcgcgccgcg tgggcg
//
LOCUS       DAOTHD010000404         1994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_67340_length_1994_cov_2.636926, whole genome shotgun sequence.
ACCESSION   DAOTHD010000404 DAOTHD010000000
VERSION     DAOTHD010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67340_length_1994_cov_2.636926"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..742)
                     /locus_tag="P5219_07320"
     CDS             complement(110..742)
                     /locus_tag="P5219_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26605.1"
                     /translation="MSTIDYSWRPEDYSEPFDPEELMLSRISGAARREVVRKMIEEGG
                     MDAVPEGFGNEELSERHRTAWGRIHPMCMGGEFLLPCEPGELEIARLTIRSTTYDVLS
                     VRAKRTKNRIRVRVDDEYDGETLNRKHMCTSARPLTLWRLLDLVITGWDVFECLEHNY
                     PDDLEGQLGFFWGESRLYPEFDEGLRERVTEYFRAHHEAEEDEENAEGEE"
     regulatory      complement(876..984)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(1036..>1994)
                     /locus_tag="P5219_07325"
     CDS             complement(1036..>1994)
                     /locus_tag="P5219_07325"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRX26606.1"
                     /translation="ACRVHARHGTTTLYPTALTAADEDMDAFFAALETAEAARKAAGA
                     SAGTRIGGVHVEGPWLAPAQAGAQDPKYLRTPSMEECEKLLARSDRIAVVTMAPELDG
                     ACECASWLAARGVLPAMGHSAADLEQVRTAVDSGFRHMVHFYSAMSTVRRENSYRIPG
                     MVEAGYLFDAVTVEAIADGHHLPLSLLRLIHKVKGPERMVLVTDAMRGAGMPEGPSVL
                     GSLKNGREVLVEGGVARLPDRSGFAGSVCTMDRAVRTAVSAGIPLEDALRMASGTPSK
                     ILGTAGRRGAVLPGMDADLVRLDGDLNVRMTVVGGTIFAGNC"
BASE COUNT          313 a          641 c          645 g          395 t
ORIGIN      
        1 ggtgggccac caccggaacc cgttgtccgt cctgaccgac cattccccgc cggccttgaa
       61 ctgtttcccg tacatccatt cgatggtgtc cctgcccgtg tacatggatt cattcctccc
      121 cttctgcatt ttcttcatct tcttccgctt catggtgtgc acggaaatac tccgtgaccc
      181 tttcccgcag accttcgtcg aattcagggt agagccggga ctctccccag aagaagccga
      241 gctgcccttc caggtcgtcg gggtagttgt gttccaggca ttcgaacacg tcccacccgg
      301 tgatcacgag gtccagcagg cgccagaggg tgaggggccg ggcggaggtg cacatgtgtt
      361 tcctgttgag ggtctcgccg tcgtattcgt cgtcaaccct gacccggatg cggttttttg
      421 tccgtttcgc ccggacggag aggacgtcgt aggtggtgga ccggatggtc agccgggcga
      481 tctccagctc gccgggttcg cagggcagaa ggaactcccc tcccatgcac atggggtgga
      541 tccgtcccca ggccgtcctg tgccgttcgc tgagctcctc gttgccgaat ccctccggaa
      601 cggcgtccat cccgccctct tcgatcattt tccggacaac ctcccgcctg gcggcgccgc
      661 tgatgcggct gagcatcagc tcctccgggt cgaagggctc ggaatagtct tcggggcgcc
      721 aggaatagtc gatcgtgctc atgggcagtt cctccttcag gaaatgggtt ccggttcagg
      781 cgaaccggta aaaaaagaca aaaaaaaggc ctctccgagg agaggcctgt ggccgccatg
      841 aggcatggac catgaatgat ccgtgcatgg gtctcctctt atcatccgcc ccgaaatcat
      901 cggggcgctg gtgttggcac cacgcctttt caggcaggtt gccgggcttc atagggccag
      961 tccctcagcc gctctggata aaagttttca gttgtcaggg tgcatattca aatcatactc
     1021 tttttttcga aaaggtcaac agtttccggc gaaaattgtg cctcccacca cggtcattcg
     1081 gacgttcaga tcgccgtcaa gcctcaccag gtcggcgtcc atgccgggga gcacggctcc
     1141 ccttcggcct gcggtgccga ggatttttga cggtgtcccg gaggccatcc ggagggcgtc
     1201 ctccaggggg attcccgccg aaacggcggt ccggacggcc cggtccatgg tgcagacgct
     1261 cccggcgaag ccgctccggt cgggaaggcg ggccacgccc ccttccacca ggacctcccg
     1321 gccgttcttc aggctgccga ggacggacgg cccttcgggc atgcccgcgc cccgcatggc
     1381 gtcggtgacc aggaccatcc gttccggccc cttgaccttg tggatgagcc ggagcaggct
     1441 cagggggagg tggtgtccgt cggcgatggc ctccacggtg acggcgtcga agaggtatcc
     1501 cgcctccacc atgccgggga tgcgatagct gttctccctc cgcacggtgc tcatggcgga
     1561 gtagaagtgg accatgtggc ggaagccgct gtcaacagcc gtccgtacct gttcgaggtc
     1621 cgcggcggaa tggcccatgg cggggaggac cccccgggcc gccagccaag aggcgcattc
     1681 gcaggcgccg tccagttccg gagccatggt gaccacggcg atcctgtccg acctggcgag
     1741 cagtttttcg cactcctcca tggagggggt gcggaggtat ttggggtcct gggcaccggc
     1801 ctgggcaggg gcgagccacg gcccctcaac gtggacgccg ccgatgcggg tgcctgcgga
     1861 ggcccccgcc gctttccggg ccgcctccgc cgtctccagg gcggcgaaga aggcgtccat
     1921 gtcctcgtct gcggccgtga gggccgtggg atagagggtg gtggtgccgt ggcgggcgtg
     1981 gacccggcag gcgg
//
LOCUS       DAOTHD010000405         1991 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_67460_length_1991_cov_3.016012, whole genome shotgun sequence.
ACCESSION   DAOTHD010000405 DAOTHD010000000
VERSION     DAOTHD010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67460_length_1991_cov_3.016012"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="P5219_07330"
     CDS             <1..438
                     /locus_tag="P5219_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HRX26607.1"
                     /translation="RIIGILGGRAAEEVVYGTKTTGAESDIEQATGLAWRMVTRWGMS
                     ERLGMVQLAPRENPYLGTQAGFGGTKPFSEETGKIIDEEVLRIIRESLDEAKRLLTVH
                     RRELDALAAALVSRETLDEKDILSVTGLTPPPAPPAAKLPAEG"
     gene            606..1694
                     /locus_tag="P5219_07335"
     CDS             606..1694
                     /locus_tag="P5219_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRX26608.1"
                     /translation="MAFERIRYEGLVELSAAVLEKLGYPANKARITAAVLAEADARGV
                     SSHGVTRLKVYRNELEGGHVKPGADPEIVHETPISLVVDGHYGVGAHISEFTMKRAIE
                     KAKAHGTCYASVRNSNHFGMAGLWAEMAVKEGLLGSAFTNTIRCAVPTLGKERLLGTN
                     PIAVGIPSDGDLPWLLDMATTTAPRGKLGVYKRRNLEMPEGWFVGESGETVTDPSEAT
                     SILADPTGRWGGLLFLGGAGELMGGHKGYGLAVLVDMLCGPLAGGPWTRDVFQTMDAN
                     VGHFFSATRLDLFGDEKDIRRRVGDMLSALRSSAPSEGNERVYVHGEKEYLRRKEALE
                     SGVPLDDATFRMLKDFAAEFGLTPPEKA"
     gene            1714..>1991
                     /locus_tag="P5219_07340"
     CDS             1714..>1991
                     /locus_tag="P5219_07340"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HRX26609.1"
                     /translation="MARITFPYEGFGDAEIPDGNLLAVLAPRTAVPASGSDLDEVKRA
                     LGEPIGSPRLREMVKKGDSALILIDDNTRNTPTARLLPLVLEELAAGGA"
BASE COUNT          430 a          613 c          638 g          310 t
ORIGIN      
        1 cggatcatag gcatcctcgg cggcagggcg gcggaagagg tagtctacgg gacaaagacc
       61 acgggagcgg aaagcgacat cgagcaggcg acggggctcg cctggagaat ggtcacccgg
      121 tggggcatga gcgaacggct cggcatggtg cagcttgcgc cccgggagaa tccctacctc
      181 ggcacccagg cgggattcgg gggaacaaaa cctttcagcg aagagaccgg aaagatcatt
      241 gacgaagagg tcctccggat catcagggaa agccttgacg aagcgaaaag gctgctgacg
      301 gtgcaccgga gggagctgga cgccctggcg gccgcccttg tctccaggga aacgctggat
      361 gaaaaggata tcctttccgt cacggggctg actccgcccc ccgcgccccc ggcggcaaaa
      421 ctgccggcag aaggctgaaa cagaggccat cccggtctga atccgtccgt accggcacgg
      481 ggcaaaaagc tcaataggca gtataatctg aggaaacacg gggaatccgg tgtttcccca
      541 tgaagtcatt ccccggattc ccccgccggg attccgggaa aaaacgcgga aggaagggat
      601 ttcttttggc ttttgagcga atacgatatg aagggctcgt tgaactctct gcggccgtcc
      661 ttgaaaaact tggctatccc gccaacaaag cccgcatcac tgccgcggtg cttgccgaag
      721 ccgacgccag gggagtctcc tcccacgggg tcacgaggct gaaggtctac cggaacgaac
      781 ttgaaggagg gcacgtcaag ccgggtgcgg accctgaaat cgtccacgaa acccccatct
      841 ccctggtggt ggacgggcac tacggcgtgg gagcccacat ctccgagttc accatgaaaa
      901 gggcaatcga aaaggcaaag gcccacggaa cgtgctacgc ctcggtgcgg aactcgaacc
      961 acttcggcat ggcgggcctg tgggcagaaa tggcggtgaa ggaaggcctt ctgggcagcg
     1021 ccttcacaaa taccatccgg tgcgccgttc caaccctggg gaaggagcgc ctcctcggca
     1081 ccaaccccat cgccgtgggc ataccctccg acggagacct cccctggctg ctcgacatgg
     1141 ccaccaccac cgctcccagg ggcaagctcg gcgtctacaa gcgccggaat ctcgagatgc
     1201 ccgagggctg gttcgtgggc gagtcgggcg aaacggtgac cgatccgtcg gaggccacct
     1261 caatcctggc ggatcccacg ggacggtggg gcggcctgct cttcctcgga ggagccggcg
     1321 agctcatggg aggacacaaa gggtacggcc tcgccgtcct ggtggacatg ctctgcggtc
     1381 ccctggcggg cggaccctgg acccgggacg tcttccagac catggacgcc aacgtggggc
     1441 atttcttctc cgccacgagg ctcgacctct tcggcgacga aaaggacatc cgcaggcggg
     1501 tcggggacat gctgtccgcc ctgcgctcct cagctccttc agaggggaac gagcgggtgt
     1561 acgtccacgg agagaaggaa tacctccgca ggaaggaggc cctggagtcg ggagtgcccc
     1621 tggacgatgc caccttcagg atgctgaagg acttcgccgc ggaattcggc ctcactcccc
     1681 ccgaaaaagc ttaaaaattg aggaggtaac gtcatggcac ggattacttt cccctacgag
     1741 ggattcggag acgcggaaat tcccgacgga aatctcctgg cggtgctcgc ccccaggacg
     1801 gccgtccctg cctccgggag tgacctggat gaagtgaaac gggccctcgg agaacccatc
     1861 ggctcgccga gactgcggga gatggtgaaa aagggagact ccgctctcat cctgattgat
     1921 gacaacacac gaaacacccc caccgcccgg ctgctccccc tggtcctcga ggaacttgct
     1981 gcagggggag c
//
LOCUS       DAOTHD010000406         1988 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_67611_length_1988_cov_2.562856, whole genome shotgun sequence.
ACCESSION   DAOTHD010000406 DAOTHD010000000
VERSION     DAOTHD010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67611_length_1988_cov_2.562856"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /gene="pheA"
                     /locus_tag="P5219_07345"
     CDS             <1..753
                     /gene="pheA"
                     /locus_tag="P5219_07345"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HRX26610.1"
                     /translation="FGGTVDPVPCPSFPDVFASVDKGLADWGVLPAENSLEGTVLPTM
                     DAFASAAKDIVIHNEVSIKVDHVLASEGTSPEDIREVLSHPQALGQCREWLRANLPRA
                     EQVPVGSTSAAAAACAGRPERAAVCSALAAERCGLSVLAEKIQDRGSNRTRFWVVGRG
                     TPRRGERNKTSFLFNVEHKPGRLFYALEPLYENGLNLTHIQSRPLSGSPFEYYFFIDV
                     EGFLEDEPVRKALDTMRERTVFLRLLGSYPCR"
     gene            complement(644..1315)
                     /locus_tag="P5219_07350"
     CDS             complement(644..1315)
                     /locus_tag="P5219_07350"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRX26611.1"
                     /translation="MTACLHDTGRDNIFIGGFMCSGKTSVGSELARRLGWTFTDTDRV
                     IEERAGMTVPEIFSILGEPAFRRYEFAAVAGALEGSRQVVSLGGGALVNEELRGMVLS
                     RAKLVILDVRPETVLSRAEKQKGARPLLDGGNVRALMIKRRPAYSHCHIRVATDEFPV
                     SSVTDRILEDLYGTAAVLWSVESPFPGITGRDRSPAAAGRPSAPSWYREPSVPAHPQG
                     TLPRR"
     gene            complement(1296..1595)
                     /locus_tag="P5219_07355"
     CDS             complement(1296..1595)
                     /locus_tag="P5219_07355"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRX26612.1"
                     /translation="MSTGQEIFIRAAMKPIPTMRKGLLSFDTVSGRPSAAHSERSDVC
                     AVPAACVVAEAMTAWIVGSMAAEQFGSDRMQDLKERFEAYRKHAERWNLHDCMPS"
     gene            complement(1592..>1988)
                     /locus_tag="P5219_07360"
     CDS             complement(1592..>1988)
                     /locus_tag="P5219_07360"
                     /inference="COORDINATES: protein motif:HMM:NF016224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HRX26613.1"
                     /translation="RAVEDMRQSMAGILSVFRPGRNRWLSALLGGKRVERILFAATKA
                     DHLHHRQHMRLTAIMAALMRDARDRADFSGAQTAAMSIASVRATVEEQVTHDGRPLDL
                     VRGRLLSTGREAAMYAGDLPENRCSSKAA"
BASE COUNT          330 a          650 c          647 g          361 t
ORIGIN      
        1 ttcggcggga cagtggatcc cgtgccgtgc ccctccttcc ccgacgtctt tgcctcggtg
       61 gacaagggac tggccgactg gggcgtgctt ccggcggaaa actcgctgga gggaacggtt
      121 ctccccacca tggacgcctt tgcctcggcg gcgaaggata tagtaataca taatgaagta
      181 tctattaagg ttgatcacgt gctcgcttcg gaagggacat cccctgagga catccgggaa
      241 gttctttccc acccccaggc tctgggccag tgcagggaat ggctccgggc gaacctgccc
      301 cgggcggagc aggttcccgt gggcagcacg agcgcggcgg cggcggcgtg cgccggacgc
      361 cccgagcggg cagccgtctg cagcgctctg gccgcggagc ggtgcggact gtctgttctc
      421 gctgaaaaaa tccaggaccg gggaagcaac aggacccgtt tctgggtggt ggggcgggga
      481 acaccccgga ggggggagcg gaacaagacc tcgttcctgt tcaacgtgga gcacaagccg
      541 gggcggttgt tctacgccct ggagcccctc tacgaaaacg gcctgaacct gacccacatc
      601 cagtccaggc ccctttccgg gagccccttc gagtactatt tcttcatcga cgtggaaggg
      661 ttccttgagg atgagccggt acggaaggct ctcgatacca tgagggagcg gacggtcttc
      721 ctgcggctgc tgggctccta tccctgccgg tgattccggg gaagggcgat tccacggacc
      781 acagcaccgc cgccgttccg tagaggtctt ccagtatcct gtcggtcacc gacgaaacgg
      841 ggaattcgtc ggtggccacc ctgatgtggc agtgggagta ggcggggcgg cgcttgatca
      901 tgagggcccg gacgttgccg ccgtccagga gggggcgggc gcccttctgc ttctccgccc
      961 gtgagagcac agtttcaggc cggacgtcca gtatgacgag cttcgcccgg gagaggacca
     1021 tgccccggag ttcctcgttc accagggcgc ctccgccgag ggagaccacc tggcggcttc
     1081 cctcaagggc gccggccacg gcggcgaatt cgtatctccg gaaggcgggt tcgcccagga
     1141 tggagaagat ctccggaacg gtcatccctg cccgctcctc gattaccctg tccgtgtcgg
     1201 tgaaggtcca tccgagcctc cgggcgagtt cgcttcccac gctcgttttt cccgagcaca
     1261 tgaacccgcc gatgaaaatg ttgtcccgtc cagtgtcatg aaggcatgca gtcatggaga
     1321 ttccaccttt cagcgtgttt tctgtaagcc tcgaaccgtt ccttcaggtc ctgcatccgg
     1381 tcgcttccga actgctccgc agccatggag cccacgatcc atgccgtcat ggcctcggcc
     1441 accacgcagg ccgccgggac tgcgcatacg tcgctccgct cgctgtgggc cgccgacggt
     1501 ctccccgaaa ccgtgtcgaa ggagagcagt cccttcctca tggtggggat gggcttcatg
     1561 gccgcccgga tgaaaatttc ctgcccggtg ctcatgccgc cttcgatgaa caccggtttt
     1621 ccggcagatc gcccgcatac atcgcggcct cccgccccgt ggagagaagc cgcccccgga
     1681 cgaggtcgag cgggcggcca tcatgggtca cctgctcctc caccgtggcg cggaccgagg
     1741 cgatggacat cgccgccgtc tgcgcaccgg agaaatcggc gcggtccctg gcgtcgcgca
     1801 tcagcgccgc catgattgcg gtcaggcgca tgtgctggcg gtgatgcaga tggtcggcct
     1861 tggtcgccgc gaaaaggatg cgttccaccc gcttgccgcc cagaagcgcg ctcagccagc
     1921 ggttccggcc ggggcggaag accgaaagga tgcccgccat cgactgccgc atatcctcga
     1981 ccgcgcgg
//
LOCUS       DAOTHD010000407         1983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_67826_length_1983_cov_3.306017, whole genome shotgun sequence.
ACCESSION   DAOTHD010000407 DAOTHD010000000
VERSION     DAOTHD010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67826_length_1983_cov_3.306017"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="P5219_07365"
     CDS             complement(<1..313)
                     /locus_tag="P5219_07365"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047514.1"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRX26614.1"
                     /translation="MGRTLVSAIIAAHSSQKAMEGEICQVKVDFAFANDITGAPAAES
                     FREMGADRVFDRERCALLPDHFTPNKDIASAEQTKKGKEFAREQGMLYWEVGRVGIEH
                     AF"
     gene            complement(294..1847)
                     /locus_tag="P5219_07370"
     CDS             complement(294..1847)
                     /locus_tag="P5219_07370"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRX26615.1"
                     /translation="MTESHRVRIFDTTLRDGEQAAGINLNKEEKLRIALQLAKMNVDI
                     IEAGFPAASPGDFEAVKLIAGTVKGPVIAGLARTRKEDIRAAAEAVRCAEKGRIHTFI
                     ATSPIHMEFKLKMTPEQVLEEVRQAVTYARSLVEDVEFSAEDGSRSDPAFLAEVFRAA
                     ADCGASTLNIPDTVGYAVPEEFGAFVQAVIEATDRPGVTWSVHCHNDLGMAVANSLAA
                     VRAGARQVECTVNGLGERAGNASLEEVVMGLRTRRDSYGCETSLDTTKLWGVSSMVSR
                     MTGFPVPPNKAVVGSNAFAHEAGIHQHGVLCNRATYEIMNPEDVGAPGSRLVLGKHSG
                     KHAFRQRVEEMGFSLSEEKITEAMALFKTLCDRKEIVTSEDLEAMINSEILAAAESRR
                     IVLKTFLVQVGRGHGTATVTLEDDGREATDAATGNGPVDAAYRAVRRIVGIEPELENY
                     SIRSVTEASDAIGEARVTLVFEGLRVSGRGASTDVIEASIRAYIDGINRLFQMAVSKG
                     VRLYGKNVG"
BASE COUNT          310 a          645 c          601 g          427 t
ORIGIN      
        1 ggaaggcgtg ctcgatgccc acccggccca cttcccagta gagcattccc tgttccctgg
       61 cgaattcttt ccctttcttt gtctgttccg ccgaggcgat gtccttgttc ggagtaaaat
      121 gatcggggag gagggcgcac cgctccctgt caaataccct gtccgctccc atttcccgga
      181 aggattccgc ggcaggggcg ccggtgatgt cgttcgcgaa ggcgaagtct accttcacct
      241 ggcagatttc accctccatg gccttctgtg atgaatgggc cgctatgatt gcgctaacca
      301 acgttcttcc catacaaacg cactcctttc gagaccgcca tctggaaaag gcggttgatt
      361 ccgtcgatat atgccctgat gctcgcttcg atgacgtcgg tgctggctcc ccggccggag
      421 acccggagtc cctcgaaaac gagggtgacc cttgcctcgc cgatggcgtc ggaagcctct
      481 gtgacggagc ggatggagta gttctccagc tccggctcga taccgacgat gcgccggaca
      541 gctctgtagg ccgcgtccac cgggccgttg cccgtggcgg cgtcggtggc ctcccggccg
      601 tcatcctcca gggtaaccgt ggcggtgccg tgccctcttc ccacctggac gaggaaggtc
      661 ttcaggacga tcctcctgct ttcggcggcg gcgaggatct cgctgttgat catggcttcg
      721 aggtcctcgg aggtgacgat ctccttcctg tcgcacaggg ttttgaaaag tgccatggct
      781 tcggtgattt tctcctcgga gagggaaaat cccatctcct ccactctctg acggaaggca
      841 tgctttcccg aatgcttgcc caggacgagc ctgctgcccg gagctcccac gtcttcgggg
      901 ttcatgatct cgtaggttgc acggttgcag agcacgccgt gctggtggat gccggcctcg
      961 tgggcgaagg cgtttgatcc gacgaccgcc ttgtttgggg ggacgggaaa gcccgtcatc
     1021 ctggacacca tggagctgac gccccaaagc ttcgtcgtgt ccagggatgt ttcgcagccg
     1081 tagctgtccc tcctggtccg aagccccatg accacttctt cgagggacgc gttcccggcc
     1141 cgttcgccaa ggccgttcac cgtgcactcc acctgcctgg cccctgcccg cacggccgcc
     1201 agggagttcg ccacggccat gccgaggtcg ttgtggcagt gcaccgacca ggtcaccccg
     1261 gggcggtccg tcgcttcgat gacggcctgg acgaaggcgc cgaattcctc gggaacggcg
     1321 tatcccaccg tgtcggggat attgagagtg gaggccccgc agtcggcggc agcgcggaag
     1381 acctcggcga gaaatgccgg gtcggatcgg ctcccgtcct cggcggagaa ctccacgtcc
     1441 tccacgaggg agcgggcata ggttaccgcc tgtctgacct cttccagtac ctgttccggg
     1501 gtcatcttca gcttgaactc catgtggatg gggctcgtgg cgataaaagt gtggattctg
     1561 cccttttccg cgcaccggac cgcctcggcc gccgcgcgga tgtcctcctt cctggtccgt
     1621 gccagcccgg cgatgaccgg gcctttcaca gtgccggcta tgagcttcac cgcctcgaag
     1681 tcgccggggg aagcggccgg aaaacctgcc tcgatgatgt ccacgttcat cttcgcgagc
     1741 tggagggcta tccgaagctt ctcttctttg ttcaggttga tcccggcggc ctgttccccg
     1801 tcccggaggg tggtgtcgaa tatacgtacc ctgtggctct ccgtcatgtc cttctctcct
     1861 ttcatccttc cccgctgccg gggataaaaa aagagccgga acctccttga gagaggttcc
     1921 ggcccgggaa tgcccttccg cagcttacca ggctgccggc gttccaggcg ggaaccggca
     1981 acc
//
LOCUS       DAOTHD010000408         1980 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_67975_length_1980_cov_2.741818, whole genome shotgun sequence.
ACCESSION   DAOTHD010000408 DAOTHD010000000
VERSION     DAOTHD010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67975_length_1980_cov_2.741818"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="P5219_07375"
     CDS             complement(<1..564)
                     /locus_tag="P5219_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRX26616.1"
                     /translation="MAGKGRTFGLLDRYILKELSGSFLFGVTAFTMVFVAGDLLFQAA
                     NLMIEQGVSLSVVTRLFVYRLPEVVAMTLPMASLLSSLLTFSRLSTNSELVALKAAGI
                     PFHRILRPVFLASILVGIGALAGNETLVPFSNRAAENLMKFEVMREKPSLLKEKVFLR
                     EESRGVLQRVIYLDQLKAREGTMKDVII"
     gene            complement(566..1039)
                     /locus_tag="P5219_07380"
     CDS             complement(566..1039)
                     /locus_tag="P5219_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HRX26617.1"
                     /translation="MTIRLLALDVDGTLTDGGVYMNGQGGEFKRFDIRDGMGIVRLKK
                     AGVEIALISGRHSAATEQRARDLGITRLHNGVADKMPLLSALVEELGITFAETAYMGD
                     DVNDVECLRAAGLALVPSDGVPEARAAADYVTFSPAGRGAVREAAELILAGNGGH"
     gene            complement(1029..1856)
                     /gene="lpxA"
                     /locus_tag="P5219_07385"
     CDS             complement(1029..1856)
                     /gene="lpxA"
                     /locus_tag="P5219_07385"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRX26618.1"
                     /translation="MTSIHPSAIVSPGAELGEDVTVGPYCIVENHVKIGSGSVLSSFV
                     TLLDYVEIGDNCRIAQNAVIGGEPQDHGFRGEESWVRIGRDVIIRENVTIHRSTGEGT
                     ATVVGDGCFIMEGVHLGHNVVIGKNVTMANKVGMAGFSSVGDGAVLGGMAGVHQFVRV
                     GKLCMIGGLSKIVKDIPPFLMADGHPADIFSLNKVGMRRAGYDSPERAAVKAFYDRLF
                     HGGLPFRKALESLASSEEGRSGAAREIIDFCTGSKRGITLWHRSRPAVRGEEDGHDD"
BASE COUNT          346 a          663 c          606 g          365 t
ORIGIN      
        1 gatgatcaca tccttcatgg tgccctcccg ggccttgagc tggtccaggt aaatcacccg
       61 ctggagaacc cccctgctct cctcccggag gaagactttt tccttgagca gggacggttt
      121 ctcccgcatg acctcgaact tcatgaggtt ctccgccgcc ctgttggaga aggggacaag
      181 ggtttcgttg ccggcgaggg ccccgatccc gaccaggatg gaggcgagga agacggggcg
      241 gagaatgcgg tggaagggga tgcccgccgc cttgagggcc acgagttcgc tgttcgtgga
      301 gaggcggctg aaggtgagca gggacgaaag caagctcgcc attggaaggg tcatggccac
      361 aacctcgggg agacggtaga cgaagagccg agtcaccacg gaaagggaga ccccctgctc
      421 gatcatgagg ttcgccgcct ggaagagcag gtcccccgcc acgaacacca tggtgaaggc
      481 cgtgacgccg aaaagaaagg agccggaaag ctccttcaga atgtacctgt cgagaagccc
      541 gaaggttctg ccctttcccg ccacgtcagt gccctccgtt tccggcgagg atgagttcgg
      601 cggcctcgcg gacagcccct cttcccgccg gcgaaaatgt cacgtaatcc gccgccgccc
      661 tggcctccgg cacgccgtcc gaggggacca gggccaggcc ggcggcccgg agacactcca
      721 cgtcgttcac gtcgtctccc atgtaggccg tctcggcgaa ggtgatcccc agctcctcca
      781 caagggcaga aagcaggggc atcttgtccg ccacgccgtt atggagcctg gtgatgccga
      841 gatcccgggc ccgctgttcc gtcgccgccg aatgccgtcc gctgatcagg gcgatttcca
      901 cgccggcctt tttcagtcgg acgattccca tgccgtcccg gatgtcgaac cgcttgaatt
      961 cgcccccctg gccgttcatg tagacgcccc cgtcggtaag agtgccgtcc acgtcgaggg
     1021 ccaggagcct aatcgtcatg cccgtcctct tcgccgcgca cagccggccg gcttcggtgc
     1081 cacagggtga tgccccgctt cgacccggtg cagaaatcga ttatttcccg ggctgccccg
     1141 ctccggccct cctcggacga cgcgaggctc tcgagggctt tccggaaggg aaggccgccg
     1201 tggaacagcc tgtcgtaaaa tgccttcacc gccgcccgtt ccggactatc gtaccccgcc
     1261 cgacgcattc ccaccttgtt gaggctgaag atgtccgccg gatggccgtc cgccatgagg
     1321 aagggtggga tgtccttcac gatcttcgac aggccgccga tcatgcacaa tttacccacc
     1381 cggacgaact ggtggactcc ggccatgccg ccgaggaccg ccccgtcgcc cacggaggag
     1441 aatcccgcca tgccgacctt gttggccatg gtgacgttct tccctatgac cacgttgtgt
     1501 cccaggtgga ccccttccat gatgaagcag ccgtcgccca ccacggtggc cgtgccttcg
     1561 ccggtggacc tgtggatggt gacgttttcc cggatgatga cgtcccggcc gattctgacc
     1621 caggattcct ctcccctgaa gccgtgatcc tggggttctc cgccgatcac ggcattctgg
     1681 gcgatgcggc agttatcgcc gatctccacg tagtcgagga gggtgacgaa ggaggagagc
     1741 accgacccgc tcccgatttt cacatggttc tcgacgatgc agtaggggcc gaccgtcacg
     1801 tcctctccga gttcggcccc cggagaaacg atcgccgaag gatggatgga tgtcatgctt
     1861 tttcggtctc ctccttctcc gaaggtgcgg ccagggtttc agccgccacg aaactgaact
     1921 ccgcctcggc ggccacggcc ccgtcgacgc ggcccacggt gcggactttg cccatcttac
//
LOCUS       DAOTHD010000409         1974 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_68265_length_1974_cov_2.778009, whole genome shotgun sequence.
ACCESSION   DAOTHD010000409 DAOTHD010000000
VERSION     DAOTHD010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68265_length_1974_cov_2.778009"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1318
                     /locus_tag="P5219_07390"
     CDS             <1..1318
                     /locus_tag="P5219_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRX26619.1"
                     /translation="RGYAGGNPVFLFLAVHCSAYLVMGLLSMSPFFGRYSHAVPFAGC
                     LFMLSLPGLLFLSEFVSSPFAASLALAGVLLAAAGVAMLIGRWGVLLSTLVPDDVAVV
                     YGLSPILSLLIIRFFSLPGVSTLAVGAPLLTLALLWGTGGVRAEETEQLAPLPGFPIW
                     RLAVFLFCFYAANGFLISLVPIVFARTEPLTDWIRVFSSLGAALVFRLYPRSDLRNFY
                     RGAFPFIAAALFFLTFSPYRTPARVFLEGGLSFLDLYAWLLLIYFASRTGLRRGAVIN
                     LGISIIVLAGTVSHFSILFRTGASLFPGQSNHMSFALLGIFLLLILLSLWDGREVTFT
                     TGGGAPGKTCEEEGFAGGDDGGDGGDADPEVKEKEEEMGRRMKLMEFNLTRQETEIAL
                     LLLKGAKDLNICSLLYISQNTLKYHLRNIYRKTGSSNRRELKNILD"
     gene            1497..>1974
                     /locus_tag="P5219_07395"
     CDS             1497..>1974
                     /locus_tag="P5219_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26620.1"
                     /translation="MPLFTGKKAGALLFLSLFIVIGLAALGAECAPQAPKNEVTRAEF
                     FLKKFEDRVNRFRGQAFRLGYEDNEALKRIKGLKEKYPDDPAVEDLFQRGRLALMKSK
                     GDFMEITPEMLVFRENEKKLKQLFGEIADREWKQFTEDILAGPTALPKAFPAPDRTQ"
BASE COUNT          374 a          582 c          542 g          476 t
ORIGIN      
        1 cagaggatac gcgggcggaa atcccgtgtt cctttttctt gctgttcact gctccgccta
       61 tcttgtcatg gggctgctct ccatgagccc tttttttggc cggtacagcc atgccgttcc
      121 ctttgccggg tgcctgttca tgctctctct tccggggctc cttttcctgt cagaattcgt
      181 atcatctcct ttcgccgcgt ccctggcgct ggcgggggtc ctcctggctg ccgccggggt
      241 tgccatgctc atcggccgat ggggggtgct cctcagcacc cttgtccccg atgacgtggc
      301 ggtggtctac ggactgtccc ccatcctctc cctgttgatt atccggtttt tctcgctgcc
      361 cggcgtttcg accctcgccg tcggagcacc cctgctgacc cttgcgcttc tctggggcac
      421 gggaggggtg cgggcggaag aaactgagca gcttgctccc cttcccggtt ttcccatatg
      481 gagactggcc gtgtttctct tttgcttcta cgccgccaac ggctttctta tcagcctcgt
      541 tcccatagtc tttgcccgga ccgaaccgct gacggattgg atcagggtct tttcctccct
      601 cggtgcggcc ctggtcttcc ggctataccc caggtcggac ctgaggaact tctacagggg
      661 cgccttcccg ttcatcgccg ccgctctttt ctttctcacc ttctcaccct atcggacacc
      721 cgcgagagtt ttccttgagg gagggctttc ctttctcgac ctctacgcct ggctgctcct
      781 gatttatttc gcatcccgaa cgggcctcag gcggggagcg gtgatcaacc tgggcatttc
      841 catcatcgtg cttgccggaa cggtttccca cttttccatt ctcttccgta ccggcgcctc
      901 ccttttcccc gggcagtcca accacatgtc ttttgccctc cttgggattt ttctcctcct
      961 catcctcctg tctctctggg acggaaggga agttaccttt acaacgggag gaggtgcccc
     1021 cggaaaaaca tgtgaggaag aaggtttcgc cgggggagac gacggcgggg atggagggga
     1081 cgcggaccct gaagtgaagg aaaaagaaga ggagatgggc cggcgaatga agctcatgga
     1141 attcaacctc acccgccagg aaacggaaat tgcccttctg ctcctcaagg gtgccaagga
     1201 tctgaatatc tgttcccttc tgtacatctc ccagaataca ctgaaatacc acctcaggaa
     1261 tatctacagg aaaacaggct caagcaacag acgggagctt aaaaacatcc tcgattgagt
     1321 gcaaaggggg tgagactcat caccccaagg ctttatggaa caggtgggaa atctcacccc
     1381 gaaaaagggg tgggaaaacc cacccttttt ttatcccatg ggtgaggacg ggctttccct
     1441 tttcgattag gctgttttcg gaaagcggaa ccagaaccgg gaggagatga tgtgccatgc
     1501 cccttttcac gggaaaaaaa gcgggtgctc ttctgtttct ttcacttttc atcgtaatcg
     1561 gcctggctgc cctgggtgcg gaatgcgccc cgcaggcacc gaaaaacgaa gtaacgaggg
     1621 cggaattttt cctgaagaag tttgaggaca gggtgaaccg tttccggggg caagccttcc
     1681 gcctcggcta cgaggataac gaggccctga agagaatcaa gggtcttaag gaaaaatatc
     1741 ccgatgatcc tgccgtggag gatctgttcc agagaggccg ccttgccctc atgaagagca
     1801 agggagattt tatggagatc acgccggaga tgctggtttt ccgggagaac gagaagaagc
     1861 tcaagcagct cttcggcgaa attgccgaca gggagtggaa gcagttcacc gaggatattc
     1921 tcgccgggcc caccgccctg ccgaaagcct ttcccgctcc cgaccggacc cagg
//
LOCUS       DAOTHD010000410         1968 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_68544_length_1968_cov_3.619446, whole genome shotgun sequence.
ACCESSION   DAOTHD010000410 DAOTHD010000000
VERSION     DAOTHD010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68544_length_1968_cov_3.619446"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /gene="rsgA"
                     /locus_tag="P5219_07400"
     CDS             complement(<1..974)
                     /gene="rsgA"
                     /locus_tag="P5219_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRX26621.1"
                     /translation="MNTIDMENLGLGRELLAGASAVYPGLFPGRIVSQSRDLYTVVAE
                     RGELTAEVSGKFRFEAGTLSHFPAVGDFVLLDRSEDAGGNGIIHAVLPRKSAFIRKAA
                     GTSNDEQVVAANIDTVFLCMSLNNDFNLRRMERYLGVAWDSGAVPVVVLTKADLCTDL
                     ARRLAEIGTVSCGAEVLVTSSLSEDGWTSVRNYLGRGKTVAFIGSSGVGKSTLINRLL
                     GWDALATKGLRNDDKGRHTTTRRELIALPEGGAVIDTPGMRELGIESADFSKAFADIG
                     KLAAECRFRDCTHSGEPGCAVRRAMDEGLLSAERLAAWKKLKKEAVYEG"
     gene            1297..1506
                     /locus_tag="P5219_07405"
     CDS             1297..1506
                     /locus_tag="P5219_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26622.1"
                     /translation="MAVAAFQEKEELRRIVDSMSPDDIRKLLDYAAFLRFLEDREDAE
                     DAAYIAAHKDEPSIPLEEALKELGL"
BASE COUNT          413 a          597 c          533 g          425 t
ORIGIN      
        1 ccctcatata cggcttcctt cttcagcttc ttccatgccg ccagccgttc cgccgaaagc
       61 agcccttcgt ccatggcccg ccggaccgcg catcccggct cccccgaatg ggtgcagtcc
      121 cggaagcggc actccgccgc cagtttaccg atgtccgcaa aggcctttga aaaatcggcg
      181 ctctcgatgc ccagctccct cattccggga gtgtcgatca ccgctccgcc ctccggaagc
      241 gctataagct cccgcctcgt ggtggtatgg cgccccttgt cgtcgttccg cagccctttg
      301 gtcgccaggg cgtcccaccc cagcagccgg ttgatcaggg tggactttcc cacgccggag
      361 gagccgatga aggccaccgt tttcccccgg cccaggtagt tccgcacgga ggtccatccg
      421 tcttccgaca ggctcgaggt caccagaacc tccgcgccgc aggacaccgt ccctatctcc
      481 gcgagcctgc gggccaggtc ggtgcacaaa tcggccttcg tcagcacgac caccgggacg
      541 gcgccgctgt cccaggccac gcccaggtag cgctccattc tccgcaggtt gaaatcattg
      601 ttcagggaca tgcagagaaa cacggtgtcg atgttcgccg ccaccacctg ttcgtcgttg
      661 gacgtccccg cagccttccg gatgaaggcg ctcttcctcg gcagcacggc atggatgatt
      721 ccgttgccgc ctgcatcttc gctccggtcg agcaggacga agtcccccac cgccgggaaa
      781 tgggataggg tcccggcctc aaaacggaac tttcccgaaa cctccgccgt cagttcgccc
      841 ctctcggcta caaccgtgta gaggtccctg gactgggaaa cgatcctccc cgggaaaagc
      901 ccggggtata ccgcagaggc cccggcgagg agttcccgcc cgagaccgag gttttccata
      961 tctatcgtgt tcacttttca ccctccaaaa gatgagattg tcgaagtctt ttgggaggcg
     1021 tatacagagc cttaccgtgt acgcacctcc cggaatgccg caatctccgg ggcgcaggtc
     1081 ttcaacatac aacaacagct ccttaagaaa gaaatatgtg ccgccgcctg atgatagcac
     1141 ccgggagccg tttttcaaca aacttttttt gtttggcgga acccgttttt gagttttttt
     1201 ccgggggtgt cttttcttcg gggaagccgt gcccggaccc ggcttgacgt ctgttctttc
     1261 atggcgtaac gtatgaaatg aaaggagttg atttccatgg ccgttgccgc ctttcaggaa
     1321 aaagaggaat tgcgcagaat agttgactcc atgagccctg atgacatcag aaagctgctt
     1381 gattacgccg cctttctccg ttttcttgaa gatcgggaag atgctgagga tgcggcttac
     1441 attgcggcgc acaaggatga gccaagcatc ccgctggaag aagctttgaa ggagcttggg
     1501 ctttgagccc gacgtattcg gtatcgatca agaaagatgc cgtcaaggca attgcggctt
     1561 ttgaccggac gtaccagcgc cgtattgctg aagttctgcg gactctggcg tctgatccaa
     1621 gaccccgggg ttgcgcacct ttgaaaggaa ttccggatac ctggcgtgtt cgtgtaggag
     1681 acatccgtat tgtctatgaa atcaatgaca gtgttctcct tgtgcacgtt gtccggattg
     1741 cccacagaag agatgtgtac aggaaattat aaaaacacct ggagacgtcg attcgttact
     1801 gcatcccgtt tctcaatccc tatccctttc tgaagacaaa caaaccccga tgcccccatg
     1861 gtgcagggaa ggtcacagaa ccgctgaagg tcgtatccga tgacggggga gcgaaatgga
     1921 gcagaaggaa aatcagaaag aaatcaacca ggcggaatgg aacaaccc
//
LOCUS       DAOTHD010000411         1967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_68605_length_1967_cov_2.646967, whole genome shotgun sequence.
ACCESSION   DAOTHD010000411 DAOTHD010000000
VERSION     DAOTHD010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68605_length_1967_cov_2.646967"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="P5219_07410"
     CDS             complement(<1..1149)
                     /locus_tag="P5219_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRX26623.1"
                     /translation="MITRSLIELVFSAASMERWNDHPHPATFTELGKQAHKMIMAWVI
                     ARYEGEERGRDIDWTALIEGGIFEFLHRVVLTDIKPPVFHKLMQDPGQREKLNSWVAD
                     RLAPDLEGLSPDFASRFRKYLLHDGDLTLERKSLRAAHYLATKWEFDFIYHWSSTKSM
                     FGIEQTRGEISRQINEHRDLRAVDEMLAARELPDRDMGLWGFVSLVGQLGFQKRWAQT
                     PRIPQTSVLGHLLFVAVLAYFISMEIGACPRRKYNNFFGGLLHDLPEVLTRDIVSPVK
                     NSVEGLDDLIKDYEKQAMEDRILPLLPEGWRQDIRYFTENEFRNRICPEGVDVPALLD
                     GDIQEEQNRNGLNPLDGRIIEACDKLAAYIEASHSIRLGLAPQALADGK"
     gene            1410..>1967
                     /locus_tag="P5219_07415"
     CDS             1410..>1967
                     /locus_tag="P5219_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRX26624.1"
                     /translation="MKGNSRTKATLLAVSAAVLLCLFAVPALAKDTLVVANIYDARTL
                     DPIVQNEVATSGMCLHIYDTLLALDQENNLVPMLAEKWEQIDDVTYKFTLRKGVKFHN
                     GEPFSAKDVKYTVERAQTPVGSAIRQYSEVVDKVEIVDDYTVIFKLKAPFTPFLMSLS
                     HTWGSIVNQKAVEAAGESYGMNPVGT"
BASE COUNT          374 a          587 c          602 g          404 t
ORIGIN      
        1 ctttccgtcg gccagcgcct gcggggcgag gccgaggcgt atggagtggg atgcctcgat
       61 ataggcggcg agcttgtcgc aggcctcgat gattcggccg tcgagagggt tcagcccgtt
      121 cctgttctgc tcttcctgga tgtcgccgtc gaggagcgcc gggacgtcca ccccctccgg
      181 acagatcctg ttccggaact cgttctcggt gaagtagcgg atgtcctgcc gccatccttc
      241 ggggaggagg gggaggatac ggtcttccat ggcctgcttc tcgtagtcct tgatgaggtc
      301 gtcgagaccc tccaccgagt tcttcaccgg ggagacgatg tcccgggtca gcacctcggg
      361 gaggtcgtgg agcaggccgc cgaagaagtt gttgtatttc ctcctggggc aggcgccgat
      421 ctccatggag atgaaatagg cgaggaccgc cacgaagagc aggtgtccaa ggaccgatgt
      481 ctgggggatc cggggagtct gggcccaccg cttctggaag cccagctgcc ccaccaggga
      541 aacgaacccc cacaagccca tgtcccggtc gggcagctcc cgggcggcga gcatttcatc
      601 caccgcccgc aggtcccggt gctcgttgat ctgcctggaa atctcgcccc tggtctgctc
      661 gatgccgaac atggatttgg tgctgctcca gtggtagatg aagtcgaact cccacttggt
      721 ggccaggtag tgggccgccc tgaggctttt gcgttccagg gtgaggtcgc cgtcgtggag
      781 aaggtacttc cggaagcggg aggcgaagtc cggggacagt ccctcgagat ccggggcgag
      841 cctgtcggcc acccaggagt tgagcttttc tctctggccg ggatcctgca tgagcttgtg
      901 gaagacgggg ggcttgatgt ccgtcaggac aaccctgtgg agaaactcga agatccctcc
      961 ctcgatgagg gccgtccagt cgatgtcccg gccccgttcc tctccttcgt accgggcgat
     1021 gacccacgcc atgatcatct tgtgggcctg ctttcccagc tcggtgaagg tggctgggtg
     1081 ggggtggtcg ttccagcgct ccatgctggc ggcggagaac acgagttcaa tcagggaacg
     1141 ggttatcacg ggaattgccc tccttttccc ggcggaaacg gccggaatat ccgttttttt
     1201 tattatacag ctcgccgggc gaccggagga aagagggggc ggggttcggt gattgtgtat
     1261 gcagtatcct tcttgacagc gaggcttccc gtggcccata atccttccgt atgcagaagg
     1321 ggatgcacca gggaaagcct gtcaagatca atgcaaaagg caactttttc ccggcctctc
     1381 tgcgtaaata tatcgaagga gatgacagaa tgaaaggaaa ctcccgcacc aaggcaacgc
     1441 ttctggcagt atccgcagca gttcttctgt gcctttttgc cgttcccgca ctggcgaagg
     1501 atactcttgt cgtggccaac atttacgacg cccgcaccct ggaccccatc gtccagaatg
     1561 aggtggccac ctccggaatg tgccttcaca tctacgacac ccttcttgcc ctcgaccagg
     1621 agaacaacct cgtccccatg ctcgccgaga aatgggagca gattgacgac gttacctaca
     1681 agttcactct ccgcaagggc gtgaaattcc acaacggcga gccgttctcg gcgaaagacg
     1741 tcaagtacac cgtcgaacgc gcccagactc ccgtcggaag cgccatccgc cagtattccg
     1801 aagttgtcga caaggtggag atcgttgacg attacaccgt catcttcaag ctgaaggctc
     1861 ccttcacccc cttcctcatg tccctgagcc acacctgggg aagcatcgtg aaccagaagg
     1921 ccgtcgaggc cgccggcgag tcctacggca tgaatcccgt gggaacg
//
LOCUS       DAOTHD010000412         1963 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_68824_length_1963_cov_2.255241, whole genome shotgun sequence.
ACCESSION   DAOTHD010000412 DAOTHD010000000
VERSION     DAOTHD010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68824_length_1963_cov_2.255241"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>993
                     /locus_tag="P5219_07420"
     CDS             <1..>993
                     /locus_tag="P5219_07420"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRX26625.1"
                     /translation="GVGSGNTPAVIDETCDIKMAVNSILLSKTFDNGLICASEQSVVV
                     CDAVYDAVAAEFTRRGAVILDEQRREMLRNILLTDGHLNTDIVGRPAWKIAALAGFEV
                     PETAKVLIGEAEVIGREEPLSFEKLSPVLGMYRAKDFDDAVEKAAALVEFAGMGHTSV
                     LYTRPENRDRIKRYSRRLSTGRVLINMPSSQGAIGDVYNFRLEPSLTLGCGSWGGNSV
                     SENVGIRHLINVKTVAERRENMLWFRIPSQVYFKPGCLGEALNELAGRKKAFLVTDRP
                     LHDLGYSGKVIAHLERLGMDVEVFCDVKPDPDLTTVNRGLERMRAFNPDAVLALG"
     assembly_gap    994..1003
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1004..1927
                     /locus_tag="P5219_07425"
     CDS             <1004..1927
                     /locus_tag="P5219_07425"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRX26626.1"
                     /translation="MWLLYENPDVKFSHLAMRFMDIRKRVCRFPSLGKKAVMVAIPTT
                     SGTGSEVTPFAVITDDAQGIKYPVADYELTPDMAIVDSDLVLSMPRSVTAASGIDAVT
                     HALEAIAATTATEYTNGIAMEALRLLFTYLPASYRDGAANPEAREQVHNAATMAGMAF
                     ANAFLGLCHSMSHKLGSAFHVAHGVANGILITEVIRFNKTGSPRKQAAFPQYKYPDAK
                     TRYARVADYLGLGGTDEDGKVENLIAAVADLRKSLDLPATISGAGVNEEEFMAKVDEL
                     SELAFDDQCTGNNPRYPLISEIRELYLRAFR"
BASE COUNT          387 a          653 c          606 g          307 t
ORIGIN      
        1 cggcgtgggg tcgggaaaca ctcccgcagt gatcgacgaa acctgcgaca tcaaaatggc
       61 ggtgaactcc atcctgctca gcaagacctt cgacaacggc ctcatctgcg cctcggagca
      121 gtccgtggtg gtctgcgatg ccgtctacga cgccgtggcg gcggagttta cccgccgggg
      181 ggcggtcatc ctcgacgaac agcggcggga aatgctccgg aacattctcc tcaccgacgg
      241 gcacctgaat acggacatcg tcggccggcc cgcctggaag atcgccgccc tcgccggctt
      301 cgaggttccc gagacggcca aggtcctcat cggcgaggcc gaggttatcg gcagggagga
      361 acccctgagc ttcgagaagc tttcccctgt gctggggatg tacagggcga aggatttcga
      421 cgacgccgtg gaaaaggccg ccgccctggt ggaattcgcc ggaatggggc acacgtcggt
      481 gctctacacc cggccggaga accgggacag gattaaacgg tacagccgca ggctctccac
      541 aggaagggtg ctcatcaata tgccgagttc ccagggagcc atcggcgacg tctacaattt
      601 ccggctggag ccgtccctga ccctgggatg cggctcatgg ggagggaaca gcgtgagcga
      661 aaacgtgggc atccggcacc tgatcaacgt gaaaaccgtg gcggaacggc gggagaatat
      721 gctgtggttc cgcatcccct cccaggtcta cttcaaaccg ggctgcctgg gcgaggccct
      781 gaacgagctt gccggacgaa aaaaagcgtt ccttgtcacc gacaggcccc tccacgacct
      841 gggatactcc ggaaaggtca ttgcccacct ggagcgcctc ggtatggacg tggaggtgtt
      901 ctgcgacgtg aagcccgatc ccgaccttac cacggtgaac cggggactcg agcgcatgag
      961 ggctttcaac ccggacgccg tcctggccct cggnnnnnnn nnnatgtggc tgctctacga
     1021 aaatccggac gtgaagttca gccacctcgc catgcggttc atggacatcc gcaagagggt
     1081 ctgccggttc ccctccctgg gaaagaaagc cgtcatggtg gccattccaa ccacctccgg
     1141 cacgggatcg gaagtgaccc ccttcgccgt gatcaccgac gacgcccagg gcatcaagta
     1201 ccccgtcgcc gactacgagc tcaccccgga catggccatc gtggactcgg acctggtgct
     1261 ctccatgccc cgctccgtga cggccgcgtc gggcatcgac gccgtcaccc acgcccttga
     1321 ggccatagcc gccaccacgg ccacggagta caccaacggc atcgccatgg aagcccttcg
     1381 cctgctcttc acgtacctcc cggcctccta ccgggacggg gccgcaaacc ccgaagcccg
     1441 ggagcaggtc cacaacgccg caaccatggc cggcatggcc ttcgccaacg cctttctcgg
     1501 gctgtgccac tccatgtccc acaagctcgg gtcggccttc cacgtggccc acggcgtggc
     1561 caacggcatc ctcatcacgg aggtgatccg gttcaacaag acgggctccc cccggaagca
     1621 ggccgccttc ccccagtaca agtaccccga tgccaagacc aggtacgccc gggtggccga
     1681 ctacctcggc ctggggggca cggacgagga cggcaaggtg gaaaatctca tcgccgccgt
     1741 cgccgacctg cggaagtccc tggacctgcc tgccaccatc tccggggcgg gggtgaacga
     1801 agaggagttc atggcgaaag tggacgagct gagcgaactg gccttcgacg accagtgcac
     1861 ggggaacaac cccaggtatc ccctgatatc cgagatacgg gagctttacc tccgggcgtt
     1921 caggtagggg aaaactgcag gccgcccaca tggaacggcg ggg
//
LOCUS       DAOTHD010000413         1961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_68909_length_1961_cov_3.159496, whole genome shotgun sequence.
ACCESSION   DAOTHD010000413 DAOTHD010000000
VERSION     DAOTHD010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68909_length_1961_cov_3.159496"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="P5219_07430"
     CDS             complement(<1..224)
                     /locus_tag="P5219_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRX26627.1"
                     /translation="MENHDRTTDCPVLADTHCHVIEGRDGYGDAGAVFTRAADAGVAR
                     MLLAGSTIPTSALAVATAEKYARLGVFAAVG"
     gene            complement(315..>1961)
                     /gene="metG"
                     /locus_tag="P5219_07435"
     CDS             complement(315..>1961)
                     /gene="metG"
                     /locus_tag="P5219_07435"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRX26628.1"
                     /translation="RTVQAIFKKLLDQGDIYKGTYQGWYCVPCEAYVPESSMGEDKTC
                     PDCRRPLQMMEEESYFFRASKYVPRLLEYYEKNLRGVMPRTRYNEIVSFLKSGVRDQS
                     ISRTTLKWGIPVPGDDAHVVYVWFDALINYVTACGYLDDPEKFGRFWPAAHHLVGKDI
                     IRFHCVVWPIMLLALGVNPPVSVFAHGWWTVEGEKMSKSRGNVVDPFEMVERYGRDPF
                     RYFLLREVPFGLDGDFSEAALVGRINSDLANDLGNLLNRTLQMVDNFCGGVLPASGPA
                     GDLEREIAELAGKTVRDVDETISDFAFDEALKAIWTLIGRGNKYIDETMPWKLAKEGK
                     EEDLHRVLNTLYDVLRLSSLLVAPFVPDTAARLWEQLGLEGDPLACSIDGFTWGERTP
                     GLKVNKGKVLFPRIDMNEWKKEKAARDEQKAAPKAPAPAPEIPLEHEEAVEIDAFRAV
                     ELRVAQILNVEEIPKSKKLYKLSVDLGYEKRTIVSGIKEFFTPEELLGKRIVVVANLK
                     PAKLCGVESNGMLLAAGDGKKTTLSLLTPDRDIPLGCRVS"
BASE COUNT          328 a          627 c          609 g          397 t
ORIGIN      
        1 cccacggcgg caaatacccc gaggcgtgcg tacttctcgg ccgtggcgac cgccagggcg
       61 ctcgtgggga tggtgcttcc cgcgaggagc atccgggcca ccccggcgtc cgccgctcgt
      121 gtgaagacgg ctcccgcatc gccgtacccg tcccgtccct cgatgacgtg gcagtgggtg
      181 tccgccagga cggggcagtc cgttgttctg tcgtgatttt ccatgatcgc ttcctcgttt
      241 tcttttcaaa aaaaccggga gtctccggcc gctccgccgg aggctcccgg gaaatccgtc
      301 ctgaaccggg gcgttcagct caccctgcag cccaggggaa tgtccctgtc gggggtcagg
      361 agggagaggg tggtcttctt cccgtcgccg gcggccagga gcatgccgtt gctctccacg
      421 ccgcagagct tggccggttt caggttggcc accacaacga tgcgcttgcc cagaagctct
      481 tccggggtga agaattcctt tatgccggag acgatggtcc gcttctcgta cccgaggtcg
      541 acggaaagct tgtagagctt cttcgacttg ggaatctcct ccacgttgag aatctgggcc
      601 acccggagct ccacggcccg gaaggcatcg atctccacgg cttcctcgtg ctccagggga
      661 atctccgggg caggggccgg tgccttgggg gcggctttct gttcgtccct ggcagccttc
      721 tccttcttcc attcgttcat gtcgatccgg ggaaagagca ctttgccctt gttcaccttc
      781 agtcccgggg tccgttcgcc ccaggtgaag ccgtcgatgg agcatgccag ggggtcaccc
      841 tccaggccga gctgctccca caggcgggcc gccgtgtcgg ggacaaaggg cgccacgaga
      901 agggaactga gcctgaggac atcgtacagg gtattcagca cacggtgcag gtcctcttcc
      961 ttcccttcct tggcgagctt ccagggcatg gtctcgtcaa tatacttgtt gcccctgcct
     1021 ataagcgtcc agatggcctt cagtgcctcg tcgaaggcga aatcagagat tgtttcgtcc
     1081 acatcccgca cggttttgcc ggcaagttcg gcaatctccc gttcgagatc tcccgccggg
     1141 cccgaggccg gaagaacacc gccgcagaag ttgtccacca tctggagggt ccggttaaga
     1201 aggttgccca ggtcgttggc gaggtcggag ttgatcctcc ccaccagggc cgcttccgag
     1261 aagtcgccgt caaggccgaa gggcacttcc cgcaggagga aataacggaa gggatcccgg
     1321 ccgtaccgct ccaccatctc gaaggggtcc accacgttcc ccctggactt ggacatcttc
     1381 tccccctcca cagtccacca gccgtgggcg aagaccgaca cgggagggtt cacgcccagg
     1441 gcgaggagca tgatgggcca caccacgcag tggaagcgga tgatgtcctt ccctacgagg
     1501 tggtgggccg ccggccagaa tctgccgaac ttctccgggt cgtcgagata gccgcaggcg
     1561 gtcacgtagt tgatgagggc gtcgaaccac acgtacacca cgtgggcgtc atcgccggga
     1621 acgggaatgc cccatttcag ggtggtccgg gagatggact ggtcacggac gccgcttttc
     1681 aggaagctca cgatctcgtt gtagcgggtc ctgggcatga ccccccggag attcttctcg
     1741 tagtattcca ggagccgggg cacgtacttc gacgcccgga agaagtagct ttcctcctcc
     1801 atcatctgga gggggcgcct gcagtccggg caggtcttgt cctctcccat gctgctctcc
     1861 ggcacgtagg cctcgcaggg cacgcagtac cagccctggt aggttccctt gtagatgtcc
     1921 ccctggtcga ggagtttttt gaagatggcc tgcaccgtcc t
//
LOCUS       DAOTHD010000414         1961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_68921_length_1961_cov_2.388248, whole genome shotgun sequence.
ACCESSION   DAOTHD010000414 DAOTHD010000000
VERSION     DAOTHD010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68921_length_1961_cov_2.388248"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="P5219_07440"
     CDS             <1..737
                     /locus_tag="P5219_07440"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRX26629.1"
                     /translation="MGSAIGMFVGAMPGAGASIACWLGYNEAKRWSRHPEEFGKGALE
                     GVAAPEATNNAVTGGAMVPLLSLGIPGSGSTAVMLGVLIIHGLRPGPMLFVTNPEIPY
                     SIFVSLFVSNVIMIFVALMLIKLLIKVVNIPETIMNACILAIIFVGAYSVNNSMFDIF
                     TVLLFGVLGLIMKIYDYPITATALGFVLGYLVETNFRRSLTLSKGDWSVFMTNPISMV
                     IIILSILSVAYAVYCNHFRRKRGAAE"
     gene            814..1896
                     /locus_tag="P5219_07445"
     CDS             814..1896
                     /locus_tag="P5219_07445"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HRX26630.1"
                     /translation="MAVYKLGMLKGDDIGLEVVPEAVKVLNAAAGRYSGVTLDWCELP
                     VGYPSYVTRGETLPASTLEALYGLEGWILGPIGHMAYPKDDPKAINPHPILRRNFDLA
                     SNIRPSRSHPSLPCLHQDVDLVIIRENNEGFQPDRNMYKGMGECMPTPDMALSLRVIT
                     RRNSSMVARTAFELARQRNGKKKVTAIHKNTVFKLGCGLFIDTCREVSREYPDVEFDT
                     AVVDTFAMHLVMRPQKFDVVVTTNMFGDILSDEAAGLVGGLGMAPGLCVGPRYAMAQA
                     THGSAPDIAGKNIANPYAMIMSAQMLLSWLGNGKGDTEAVRAAKDIETALGMVLSDKE
                     SVTPDLGGTAGTDAMGDAVCKALASL"
BASE COUNT          414 a          588 c          586 g          373 t
ORIGIN      
        1 tcatgggatc ggccatcggc atgttcgtgg gtgccatgcc gggagcggga gcatccatag
       61 cctgctggct cggctataac gaggcaaaac ggtggtccag gcaccccgag gagttcggca
      121 agggcgccct ggaaggagta gcggccccgg aagcgacgaa caacgccgtg acgggagggg
      181 ccatggtacc tcttctttcc ctgggcatac cgggctccgg ctcgacggcg gtcatgcttg
      241 gagtgctgat cattcacggt cttcgtccgg gccccatgct cttcgtcacc aacccggaaa
      301 tcccctattc gatcttcgtc tccctgttcg tctcgaacgt catcatgatc ttcgtcgccc
      361 tgatgctgat caagctcctg atcaaggtgg tcaacatccc cgagactata atgaacgcct
      421 gcatcctggc catcattttc gtcggggcct actcggtgaa caacagcatg ttcgacatct
      481 tcacggtcct tctcttcggt gtcctcgggc tgatcatgaa aatttacgat taccccatta
      541 cggccacggc cctcggtttc gtgcttggat acctcgtgga gacgaatttc aggaggtccc
      601 tcaccctttc caagggcgac tggtctgttt tcatgacaaa ccccatctct atggtgatca
      661 tcatcctttc catcctgagc gtggcctacg cggtctactg caaccacttc aggagaaaac
      721 ggggggcggc ggaatagaaa acccctgccc ggaacgctcc tggccgggaa aaatgacaag
      781 atactccaaa aatatgcgag ggagagtcga gacatggcgg tctacaagct tggaatgctg
      841 aaaggcgacg atattgggct ggaagtggtg ccggaggccg taaaggtgct gaacgccgcc
      901 gccggaaggt acagcggcgt gacgctggac tggtgcgagc tgccggttgg gtacccctcc
      961 tacgtcacaa gaggggagac ccttccggcc tcgacgctgg aagccctgta cgggcttgaa
     1021 ggatggattc tcggccccat aggccacatg gcttacccga aggacgaccc caaggccatc
     1081 aatccacacc ccatccttcg gcggaatttc gacctggcga gcaacatccg gccgtcacgg
     1141 tcccaccctt ccctgccgtg tcttcaccag gacgtggacc tggtcatcat cagggaaaac
     1201 aacgaggggt tccagcctga caggaatatg tacaaaggca tgggggagtg catgcccaca
     1261 ccggacatgg ccctgtccct ccgggtgatc accaggagaa attcctccat ggtggccagg
     1321 acggccttcg aactggcccg gcagcggaac ggaaagaaaa aggtcacggc gatccacaaa
     1381 aatacggtct tcaagctggg atgcggcctc ttcatcgata catgcaggga agtgagcagg
     1441 gagtaccccg acgtggaatt cgacacggcg gtggtggaca cctttgccat gcacctcgtc
     1501 atgcggccgc agaaatttga cgtggtggtg accacgaaca tgttcggcga catcctctcc
     1561 gacgaagcag ccggccttgt gggcggtctc ggcatggccc ccggcctctg cgtcggtccc
     1621 cggtacgcca tggcccaggc cactcacggg tcggcgccgg acattgcggg gaagaacatc
     1681 gccaacccct acgccatgat catgtccgcc cagatgctgc tctcgtggct cgggaacggc
     1741 aaaggagaca cggaagcggt ccgggcggcg aaagacatag aaactgccct cggaatggtg
     1801 ctctccgata aagaaagcgt taccccggac ctcgggggga cggcggggac cgatgccatg
     1861 ggcgatgccg tctgcaaggc cctcgcgtcc ctgtagcgga agctgataac gaaagagccc
     1921 cttcccggag aatccgggaa ggggctcttt tcgcagggtt g
//
LOCUS       DAOTHD010000415         1959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_68984_length_1959_cov_3.470063, whole genome shotgun sequence.
ACCESSION   DAOTHD010000415 DAOTHD010000000
VERSION     DAOTHD010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68984_length_1959_cov_3.470063"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /gene="tuf"
                     /locus_tag="P5219_07450"
     CDS             <1..1082
                     /gene="tuf"
                     /locus_tag="P5219_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HRX26631.1"
                     /translation="AEFTAYDMIDKAPEERERGITINIAHVEYQTDNRHYAHIDCPGH
                     ADYIKNMITGAAQMDGAILVVSAADGPMPQTREHVLLARQVNVPALVVFMNKTDQVDD
                     PELLDLVEMEVRELLSKYEFPGDDIPVIRGSALKVMEEGNGTREDPISKCIWELMDAC
                     DSYIPAPVREVDKPFLMPIEDVFTITGRGTVVTGRVERGVIKGGEEVAIVGMKTDVTK
                     TVATSLEMFRKILDSAMAGDNVGVLLRGVDKDGVERGQVLAKPGSITPHTKFKAEVYV
                     LKKEEGGRHTPFFAGYKPQFYFRTTDVTGGIKLPEGVEMVMPGDNATFEVDLIVPIAM
                     DPGLRFAVREGGHTVGAGVVTEILE"
     gene            1108..1257
                     /gene="rpmG"
                     /locus_tag="P5219_07455"
     CDS             1108..1257
                     /gene="rpmG"
                     /locus_tag="P5219_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRX26632.1"
                     /translation="MADIVGLSCTECKRRNYTTTVNKKKQSKKLELNKFCKWCGKHTL
                     HKEGK"
     gene            1279..1354
                     /locus_tag="P5219_07460"
     tRNA            1279..1354
                     /locus_tag="P5219_07460"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1312..1314,aa:Trp,seq:cca)
     gene            1393..1575
                     /gene="secE"
                     /locus_tag="P5219_07465"
     CDS             1393..1575
                     /gene="secE"
                     /locus_tag="P5219_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRX26633.1"
                     /translation="MENILTFVREARAELKKVTWPGKKQVWYSTLVVIAFTLFVSAYL
                     GLVDLLLTGVLSRLFS"
     gene            1663..>1959
                     /locus_tag="P5219_07470"
     CDS             1663..>1959
                     /locus_tag="P5219_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HRX26634.1"
                     /translation="MDSIEERRWYIVQTYAGYENRVKANLEQRIATMGMEDRIFSVLV
                     PVEERVSVKDGKSKKVTRKLFPSYVLVEMRLNDQSWYVVRHTPGVTGFVGSGNHP"
BASE COUNT          459 a          507 c          602 g          391 t
ORIGIN      
        1 atgcggaatt cacggcctac gacatgatcg acaaggctcc cgaagagcgt gagcgcggca
       61 tcaccatcaa catcgcccac gtggagtacc agacggacaa ccgtcactat gcccacatcg
      121 actgcccggg ccacgcggac tacatcaaga acatgatcac gggagcggcc cagatggacg
      181 gagccatcct cgtggtgagc gcggcggacg gtcccatgcc ccagacccgt gagcacgtgc
      241 ttcttgcccg ccaggtcaac gtgccggccc tcgtggtctt catgaacaag acggaccagg
      301 tggatgatcc cgagcttctt gaccttgtgg aaatggaagt tcgtgagctg ctgagcaagt
      361 atgaattccc cggagacgac atccccgtca tccgcggctc ggcgctgaag gtcatggaag
      421 aagggaacgg cacccgtgag gatcccatca gcaagtgcat ctgggagctc atggatgcgt
      481 gcgacagcta catccccgcg cctgtgcgcg aagttgacaa gcctttcctc atgcccatcg
      541 aggacgtgtt caccatcacc ggccgcggca ccgtggtcac gggcagggtg gagcgtggag
      601 tcatcaaggg cggcgaagaa gtagccatag tgggaatgaa gacggacgtc acgaagacgg
      661 tggcgacgtc ccttgaaatg ttccgtaaga tcctggacag tgccatggcg ggagacaacg
      721 tgggagtgct gcttcgtgga gtggacaagg acggagttga gcgtggccag gtactggcga
      781 agccggggtc gatcacgccg cacacgaagt tcaaggcaga agtgtacgta ctgaagaagg
      841 aagagggcgg acgacacacg ccgttctttg cggggtacaa gccccagttc tacttccgga
      901 ccacggacgt gacgggtggc atcaagcttc ccgagggagt ggaaatggtc atgccgggag
      961 acaacgcgac gtttgaagtg gacctgatcg tgcccatcgc catggacccg ggtcttcggt
     1021 ttgcggtgcg cgagggcggc catacggtcg gcgccggcgt cgtcaccgag attcttgaat
     1081 aattaaactt cgaggaggca accatttatg gcggatatcg tcggcctctc ctgcacggag
     1141 tgcaagaggc ggaactacac cacgactgtg aataagaaga agcagtcgaa gaaacttgag
     1201 ctcaataaat tctgcaagtg gtgcggcaag cacaccttgc acaaagaagg caagtagtgt
     1261 aaaatacact ctgctcgcag gtccgtagct caattggcag agcactggtc tccaaaaccg
     1321 ggggttgcag gttcgagtcc tgccgggcct gccatttttt tattgtcatt aaaaagatag
     1381 gaggccgagg ttgtggaaaa tatcctcacc ttcgtacgag aagcccgagc cgaattgaaa
     1441 aaggtaacat ggccaggaaa aaagcaggtg tggtattcca cgctggtggt gatcgctttc
     1501 accctttttg tttccgccta ccttggactc gtggatttgc ttttaacggg agtactgtca
     1561 cgtcttttca gttaatgtac gctgccggtc caggcagttg cgttccagga tcacttcaga
     1621 gatcggcagg gaggtgaggg ggccgcgaga cccccttcat gcatggatag cattgaagag
     1681 cgtcgctggt atatagttca gacctatgca ggatacgaga accgcgtgaa ggcgaatctc
     1741 gaacagagga tcgccacaat gggcatggag gacaggattt tttccgttct tgtgcctgtt
     1801 gaggagcgtg tgtccgtgaa ggacgggaaa agcaagaaag tgacccggaa acttttcccc
     1861 agctatgttc tcgttgaaat gcgtcttaac gaccagtcgt ggtacgtggt gcgccatact
     1921 cccggtgtca cgggttttgt cggatccggt aaccacccc
//
LOCUS       DAOTHD010000416         1955 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_69171_length_1955_cov_3.060526, whole genome shotgun sequence.
ACCESSION   DAOTHD010000416 DAOTHD010000000
VERSION     DAOTHD010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69171_length_1955_cov_3.060526"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            358..1557
                     /locus_tag="P5219_07475"
     CDS             358..1557
                     /locus_tag="P5219_07475"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRX26635.1"
                     /translation="MWNKGEGVVLKFGGTSVANPERIRAGAGRIAGFFRSGLRVAVVV
                     SAMGSTTNSLIALSGETSGGTPDRRELDQLLATGEQQSAALMAMELGRQGCPAVSFTG
                     AQAGFLAGGPWGEGRILSVNPVRVEEACASGRVAVVTGFQAATASGDTITLGRGGSDL
                     SAIALAGALGADSCRIFTDVDGVFSADPRKVPGALRIGRISWEECLEMTFLGAKVMQS
                     RSIEMAERLELPLWVGSGEGEGTWIMGRTVDEAFRACAVVSDENIALLSAEGCGNGGC
                     DVAEEFFLRGIHILAAAERGCPLFYIRRERLEEAAEICRRMSGKSPSVNPGAARVSVV
                     GPGAGNHPEIGRAMLKVLGRLGAPAHLLTSSGLSVSCFVDEKDAQPALRALHEEFIEK
                     KGEFQCA"
     gene            1548..>1955
                     /locus_tag="P5219_07480"
     CDS             1548..>1955
                     /locus_tag="P5219_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRX26636.1"
                     /translation="MRVEKEWGLCFVGFGNVGQGLARLLVKKEQELASRWGFSCRTLA
                     VVGKTKGALVRPEGIGLADLLLRVGRGEPLGSDSVSPVEAALLPGVDMVIDVTPTNLK
                     DGEPGLSLTRAALEAGRHVVTSDKGPVSLAFPEL"
BASE COUNT          329 a          553 c          710 g          363 t
ORIGIN      
        1 tctgcaccgg cggcccaagc gagcaccggt gacgctcccc gctgcctgcg gatttgggcg
       61 ccgcaaatgc acttgatttt ctctctgctt tgctaaaaat gtgaagtttt cccgttgaca
      121 gggggccttt cggggcgtat catccttgtc aatttcagcc tgaccggaaa ggagacgccg
      181 ccgtgagcat gaaaccagca gttccccatc atcccgcagc ctgtgccatg acgatgacta
      241 gcggcgaccc tcctggagag gtcggcgcct gatccgtcgt gcaccgacag cttcagccgg
      301 gggtcttcgt acagaaggcc cccggctttt ttttattctg cgaaaggggg aaagcccgtg
      361 tggaacaagg gagaaggcgt agtgctcaag ttcgggggaa cgtcggtggc gaaccctgaa
      421 aggatcaggg ccggggcagg ccggatcgcc ggttttttcc gctcgggtct cagggtggcc
      481 gtggtggtgt ccgccatggg aagcaccacc aacagcctga tcgctctctc gggtgaaacc
      541 tcgggcggga cgccggacag aagggagctg gaccagcttc ttgccacggg ggagcagcag
      601 tcggcggccc ttatggccat ggagctcgga aggcagggat gccctgcggt ttccttcacc
      661 ggcgcccagg cgggatttct cgccgggggt ccctgggggg aagggcgcat cctgtccgtc
      721 aacccggtcc gggtggaaga agcctgcgcc tccgggcggg ttgccgtggt gacgggcttc
      781 caggcggcta cagcctcggg tgacaccatc accctggggc ggggcgggtc tgacctttcg
      841 gccatcgccc tggcgggagc cctgggagcg gattcatgcc gcattttcac cgacgtggac
      901 ggggtattct cggcggatcc ccggaaggtc cccggggcgt tgaggatcgg gcggatctcc
      961 tgggaggaat gcctcgaaat gactttcctc ggggcgaagg tgatgcagtc ccggagcatc
     1021 gagatggcgg aacggctgga gctgcccctg tgggtgggct ccggcgaagg ggagggaacg
     1081 tggatcatgg gaagaacggt ggatgaggcg tttcgggcgt gtgctgtggt gtcggacgaa
     1141 aatatcgccc ttctttcagc ggagggatgc gggaacggag gctgtgacgt ggcggaggag
     1201 tttttcctgc ggggcatcca tattctggcc gccgcggaac gggggtgtcc cttgttttat
     1261 atccgccggg agcggctgga ggaagcggcg gagatatgcc gcaggatgtc ggggaaatct
     1321 ccatcggtga accccggtgc ggcgagggtc tccgtggtgg ggcccggggc gggaaaccac
     1381 cccgagatcg gcagggccat gctgaaggtc ctcggccgac tcggcgctcc ggcgcacctt
     1441 ctgacctctt cggggctctc cgtaagctgt tttgtggatg aaaaggacgc gcagcccgcg
     1501 ctccgcgccc tgcacgaaga attcatcgaa aagaaagggg aatttcaatg cgcgtagaaa
     1561 aagaatgggg gctgtgcttc gtggggttcg gcaacgtggg tcaggggctg gcccggctgc
     1621 tggtgaaaaa ggaacaggag ctcgcttcca ggtggggctt ttcatgccga acgctggccg
     1681 tagtgggaaa gacaaagggc gccctggtcc gccccgaagg gatcggcctg gcggatcttc
     1741 tgctcagggt cggccggggg gagcccctgg gctcggactc cgtttctccg gtggaggcgg
     1801 cgctgcttcc cggagtggac atggtgatcg acgtgacccc caccaacctg aaggacggcg
     1861 agccgggcct gtcgctcacc cgggccgctc tcgaagcggg acggcacgtg gtaacatccg
     1921 acaagggtcc cgtctccctg gccttcccgg agctg
//
LOCUS       DAOTHD010000417         1955 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_69189_length_1955_cov_2.374211, whole genome shotgun sequence.
ACCESSION   DAOTHD010000417 DAOTHD010000000
VERSION     DAOTHD010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69189_length_1955_cov_2.374211"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /gene="hcp"
                     /locus_tag="P5219_07485"
                     /gene_synonym="priS"
     CDS             <1..639
                     /gene="hcp"
                     /locus_tag="P5219_07485"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HRX26637.1"
                     /translation="DRPKGGMKDFSSMIEMAKKCAAPREIETGKLVGGFAHNQVMALA
                     DKVVAAVKSGAIKRFVVMAGCDGRFKSRDYYTDVAKNLPKDTVILTAGCAKYRYNKLD
                     LGDIGGIPRVLDAGQCNDSYSLAVIALKLKEAFGLDDVNKLPISYDIAWYEQKAVAVL
                     LALLSLGVKGIRLGPTLPGFLSPNVADVLVKNFDIKPISTVEEDVAAMMAGK"
     gene            complement(826..>1955)
                     /locus_tag="P5219_07490"
     CDS             complement(826..>1955)
                     /locus_tag="P5219_07490"
                     /inference="COORDINATES: protein motif:HMM:NF013784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HRX26638.1"
                     /translation="VVIGEDVITKDGDLVVENGLVQSSPLMEAMLSAFRRYHRGYGDI
                     ILQANYDDERHHVLEHGITRLGVKSVELKFGQGAKGIQGMNRITFEEALKFKKRGYMI
                     YPDPTDPRVAENHAKGIGQVFERLGKLPFWDEDFLVRRVAELRNLGAERVCFKVGPFD
                     PRDLLRILKAASQAGVDMVTFDGGGGGSGSSPAKMMNEWGIPTVYMESLLYDMLKKMD
                     EKNYPLPQVVIAGGFALEDQIYKGLALGAPYIGLVGVGRAAMAAAMTGKQIGKMIEEG
                     NVPKNLQAFGSTRDEIFEGVRELKEFYGNDVKDMSTGAIGVYSYLRRISAGLRQMMGL
                     NRKFTLKHIGRSDIVPLTELAAQVTGLKTYKEIADEEIAKL"
BASE COUNT          374 a          617 c          544 g          420 t
ORIGIN      
        1 gacaggccga agggcggcat gaaggacttc tcctccatga tcgagatggc gaagaagtgc
       61 gccgctccca gggagatcga gacgggcaag ctcgtcggcg gtttcgccca caaccaggtc
      121 atggcgctcg ccgacaaagt cgttgcagcg gtgaagtccg gcgcgatcaa gcgtttcgtc
      181 gtcatggcgg gatgcgacgg ccgcttcaag tcccgcgact actacaccga cgtcgccaaa
      241 aacctgccga aggacaccgt gatccttacc gccggctgcg ccaagtaccg gtacaacaag
      301 ctggacctcg gcgatatcgg cggcattccc cgggtgcttg acgccgggca gtgcaacgac
      361 tcctattccc ttgccgtgat cgccctcaag ctcaaggagg ccttcggcct cgacgatgtc
      421 aacaagcttc ccatctccta cgacatcgcc tggtatgagc agaaagccgt ggcggtcctt
      481 cttgccctgc tctccctcgg agtcaaggga atccgcctcg ggccgaccct tcccgggttc
      541 ctttccccca atgtggccga tgtgctggtg aagaacttcg acatcaagcc catcagcacc
      601 gtggaggaag acgtcgccgc aatgatggcg ggcaaataga ccggggtaca tgcttcagcc
      661 tgggcccatt ccggagaaag ccgggcgtcc gcggcttgcc ggaaggccgc acaggaactg
      721 ctgcatcact aaaaaacgcc ggagagcgga tcaaccgctc tccggcgttt ttattcctgc
      781 ccggaacgcc tgttcccgtc catacggaac gggccggtaa tgaaattaca gctttgcgat
      841 ctcctcgtcg gcgatctcct tgtaggtctt caggcccgtc acctgtgcgg cgagctcggt
      901 gagggggacg atatcgcttc tcccgatatg cttcagggta aattttctgt tcaggcccat
      961 catctgccgc agcccggcgg aaattctgcg gaggtaggag tacacgccga tagcccccgt
     1021 tgacatgtcc ttcacatcgt tcccgtagaa ctccttcagc tcccggacgc cctcgaagat
     1081 ttcatcccgg gttgagccga atgcctggag attcttgggg acgttccctt cctcaatcat
     1141 cttgcctatc tgtttgcccg tcatggcggc ggccatggct gcccggccga cacccacgag
     1201 gccgatatag ggcgcaccga gggccagccc cttgtagatc tggtcttcca gggcgaaacc
     1261 tcccgctatg acgacctggg gaagggggta gtttttctcg tccattttct tcagcatgtc
     1321 atagagcagg ctctccatgt agacggttgg gattccccat tcattcatca tcttggcggg
     1381 gctgctgccc gagccgcccc cgcccccgtc gaaggtcacc atgtcgacgc cggcttgtga
     1441 cgctgccttg agaatcctca gaagatcccg gggatcaaag gggccgactt tgaaacatac
     1501 ccgctccgct ccgaggtttc tgagttccgc gaccctccgg accagaaaat cctcgtccca
     1561 gaagggaagc ttccccagcc gctcaaagac ctggccgatg ccctttgcat ggttttccgc
     1621 cacccgggga tcagtggggt cgggatagat catataccct cttttcttga acttcagggc
     1681 ctcttcaaag gttatgcggt tcatcccctg gatgcccttt gccccctggc cgaatttcag
     1741 ctccacggat tttacgccga gcctggtgat tccgtgctca aggacgtgat gcctctcgtc
     1801 gtcgtaattg gcctgaagga tgatgtctcc gtatcccctg tggtatctgc ggaaagccga
     1861 cagcattgcc tccattaaag gagaggactg gacgagcccg ttttcaacga cgaggtcccc
     1921 gtccttggtt ataacgtctt cgccgataac aacgg
//
LOCUS       DAOTHD010000418         1954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_69227_length_1954_cov_2.606108, whole genome shotgun sequence.
ACCESSION   DAOTHD010000418 DAOTHD010000000
VERSION     DAOTHD010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69227_length_1954_cov_2.606108"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="P5219_07495"
     CDS             complement(<1..220)
                     /locus_tag="P5219_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_133957783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SYNERG-CTERM system CAAX-type protease"
                     /protein_id="HRX26639.1"
                     /translation="MKELLVFAFFLYFPYGWCALSGNSRESFGLIWKADRHALRDVAA
                     VSLLTLLPLTAVSLFFFGDRLFPPALRSV"
     gene            complement(217..480)
                     /locus_tag="P5219_07500"
     CDS             complement(217..480)
                     /locus_tag="P5219_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26640.1"
                     /translation="MNLDALTDFSFLRPLAPLLFLLFFLAAALPFLRRPGKERLFYAA
                     LGLFFFGASAVFLLMGQPLAAFSVFFVPALLYSVFRKAGRKNK"
     gene            728..>1954
                     /locus_tag="P5219_07505"
     CDS             728..>1954
                     /locus_tag="P5219_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26641.1"
                     /translation="MNRSFFLEETDLTLDVVILLISGVMMVLTGILLFPVTRGAIPYY
                     ENGVYGLVLFLFALQIILLGQTPFGEMKRSKPLFCTGLAVASAGIVTCFIPGIFSRLP
                     AVLLFLCFTPGGAALLAHLFLAPGRFRTWARMGGTFHLLWTGCAAVYLLSMLIGVLVL
                     NQGLFSGDETAVTVLVFGAAILFLAATISKIYRKYPAPEGKESESEILSSEHSVILLT
                     GIFMILLGFFLVPVSLGMLPFAGSAQLGLLMVIMALKMISTGNTPIGAFRRTRLVVIC
                     GVLFAGLGIVSCIVPGILVSLLTLLIGLLNIFGGIIGLRGTLSGLRKQGRNPVLPVLV
                     RLNISQVVLNLLSIMFGTSMLFPGLLPALLTGLILAANGGVLLYLLHILILLEKMAGT
                     AAESPHSGGTAGMSRSN"
BASE COUNT          440 a          551 c          523 g          440 t
ORIGIN      
        1 gaaccgaccg cagggcaggg gggaaaagac ggtccccaaa aaagaaaagg gagacggccg
       61 tcaggggcag cagggtcaga agagaaactg ctgcaacgtc ccgaagggca tggcggtctg
      121 ctttccagat caacccgaag gattccctgc tgtttccgga cagggcgcac cagccgtaag
      181 ggaaatagag aaaaaaagcg aagaccagca attccttcat ttgttcttcc gtcccgcttt
      241 cctgaatacg ctgtagagaa gggccggaac aaaaaacacg gaaaaagcgg caaggggctg
      301 ccccatcaga agaaaaacag ccgatgcccc gaaaaagaaa agcccgagag cggcataaaa
      361 gagccgttcc tttccgggac ggcgcagaaa aggaagggct gccgcaagaa aaaacagcag
      421 aaacagtaag ggggcaagag gccgcagaaa ggaaaaatcg gttagagcat ccaggttcat
      481 gagtatctcc ttttatctcg gcaaccgggt ccggatgggc attgacggac aacgaccgcc
      541 cgcagcaggc tttcttccgg ccattgtatc atttctctcc ccggcctgag cggcagacag
      601 aaatctttcc cgtcagagag gtgaaaaaaa agagaatgga gcgtaagata gagaaaatta
      661 cctcagccgc ccgaaacgcc tcccttcagg aacggctggt tcagggagaa agaaaggagc
      721 gggttcgatg aacaggagct ttttccttga agagacggac cttacgctgg acgtggtgat
      781 tcttctcatt tccggggtga tgatggtcct gaccggcatc ctgctttttc ccgtgacgag
      841 gggagcaatt ccatactatg aaaacggggt ctacggtctt gtactcttcc tctttgccct
      901 gcagatcatt ctcctggggc aaacgccctt cggggagatg aaacggtcaa aacctctctt
      961 ctgcaccggt ctcgcggtgg cgtcagccgg aatagtgacc tgtttcattc caggcatttt
     1021 cagccgcctt cctgctgtat tgctctttct ctgcttcact cccggagggg cagccctttt
     1081 ggcccacctt tttcttgccc ccggcagatt ccgtacctgg gcccgcatgg gaggaacgtt
     1141 tcatctcctg tggacggggt gcgcggccgt ctatcttctt tccatgctta taggggtgct
     1201 cgtgctgaat cagggactgt tttccgggga tgaaactgcc gtcaccgttc ttgttttcgg
     1261 tgcggccatc ctttttctgg cggcgacaat atcaaagatt tacaggaaat atcccgcccc
     1321 cgaaggaaaa gagagcgaga gtgaaatctt atcctccgag cattccgtca ttctccttac
     1381 gggcatcttc atgatccttc tcggcttctt tctcgttccc gtaagcctcg gcatgcttcc
     1441 tttcgccggg agtgcccagc tcggcctcct catggtgata atggccctta aaatgatatc
     1501 aacgggcaac acccccatag gtgccttccg ccgcacccgg ctggtggtaa tatgcggagt
     1561 ccttttcgct ggcctgggca tcgtttcctg catagtgccc ggaattctcg tttcccttct
     1621 gactcttctc atcggtctgc tgaacatatt cggcgggatc atcggactcc ggggaaccct
     1681 ttcggggctc cggaaacagg gaagaaatcc agttttgcct gtcctggtac gtctgaacat
     1741 ctcccaggtc gtcctgaatc tgctctccat catgttcgga acctccatgc ttttccccgg
     1801 actgcttccc gccctcttga ccgggctgat ccttgcggcc aacggcgggg ttctcctcta
     1861 cctgctccat atcctgattc ttctcgaaaa gatggccgga acagcggcag aaagccccca
     1921 ttcaggggga accgccggaa tgtcccggtc caac
//
LOCUS       DAOTHD010000419         1952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_69310_length_1952_cov_2.711123, whole genome shotgun sequence.
ACCESSION   DAOTHD010000419 DAOTHD010000000
VERSION     DAOTHD010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69310_length_1952_cov_2.711123"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="P5219_07510"
     CDS             <1..425
                     /locus_tag="P5219_07510"
                     /inference="COORDINATES: protein motif:HMM:NF020376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HRX26642.1"
                     /translation="TPDRVRPGKKEECGLDTVHFITITGIDHYYGKTPFEIGRIARLS
                     KEPENPHDPEAIRVELPVIGTIGYVANSTNTVYRGTASAGRIYDKMGDYAFARVFFVT
                     HSSAIALVLSPDEVEEIDSGKKVIFTVNDEEEPPEADG"
     gene            complement(518..1786)
                     /gene="larA"
                     /locus_tag="P5219_07515"
     CDS             complement(518..1786)
                     /gene="larA"
                     /locus_tag="P5219_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HRX26643.1"
                     /translation="MDFQLKRGRGTEAFTLEDSKILQVVLPPETNLKPCTLRDVEDAL
                     DSPVGTPPLLELLERKKPRKVVLIVNDITRPTPYGLLLPPVLARFARAGITDDMVTLL
                     VATGIHDPHTDEQNLQVYGEETVRRFRIVSHIADDMDSLVSAGTLSTGSELKINRLVA
                     EADFVLTLGVVMPHYFAGYSGGRKSILPGVAWKETVERNHSRMVELMDALPDLDSNPV
                     NLEMIEAARMVGVDFILNVVVNEKKDVVKVAAGDVVKAWREAVAVSASLYEVPVKGLA
                     DVAVAGACGHPRDINMYQAQKALDHADKATKKGGAIVLVADCPGGYGEAVFEEWMNAA
                     SCPEDIVRRIRTDFKMGGHKAFGFAKVAAEKTVYMVTSMDGAEVKKLFAVKVPSVEDA
                     LRLIEHERGKDLSYMVLPEGSLTVPVPPRS"
BASE COUNT          350 a          668 c          587 g          347 t
ORIGIN      
        1 ataccccgga cagagttcgc cccgggaaaa aggaggaatg cgggttggat acggtccact
       61 tcatcaccat cacggggatc gaccattact acggcaagac accctttgaa atcggacgga
      121 tcgccaggct ctccaaggag ccggaaaacc cccacgaccc ggaggccatc cgcgtggagc
      181 tgccggtcat cggcaccatc ggctacgtgg cgaacagcac gaacaccgtc taccggggta
      241 cggccagcgc ggggcgcatc tacgacaaga tgggggacta cgccttcgcc cgggtcttct
      301 tcgtcaccca ctccagcgcc attgccctcg tcctctcgcc ggacgaagtg gaggagatcg
      361 actcaggcaa aaaggtcatc ttcaccgtga acgacgagga ggagcccccg gaggccgacg
      421 gctagcgagg ggccgctccg cccggaccct gtcgtgaaaa aaacgggcct tcccttccgc
      481 agatcgcggc gggaaggccc gttcggttct ttcgttttca gctccggggc ggaaccggga
      541 ccgtgaggct tccctccggc aggaccatat aggagaggtc cttcccccgc tcatgctcga
      601 tgagccgcag ggcatcctcc accgacggca ctttcacggc gaagagcttt ttcacttccg
      661 cgccgtccat ggacgtgacc atgtagaccg tcttctccgc ggcgaccttg gcgaagccga
      721 aggccttgtg gcctcccatc ttgaaatcgg tccggatgcg cctgacgata tcctccggac
      781 aggacgcggc gttcatccac tcctcgaaca cggcctctcc gtaccctccg ggacagtctg
      841 ccacgagcac gatggcgccg cccttcttcg tggccttgtc ggcatggtcg agggccttct
      901 gggcctggta catgttgatg tcccggggat ggccgcaggc gccggcgacg gccacgtccg
      961 caagcccctt caccggaacc tcgtacaggc tcgccgagac ggcgacggcc tcccgccacg
     1021 ccttcaccac gtcaccggcg gcgaccttca ccacgtcctt cttctcgttg accaccacgt
     1081 tgaggatgaa atccaccccc accatccggg ccgcctcgat catctcgagg ttcaccgggt
     1141 tggagtccag gtccgggagg gcgtccatga gctccaccat ccgggaatgg ttccgctcca
     1201 ccgtctcctt ccacgccact ccgggcagga tggacttccg cccgcccgag tagcccgcga
     1261 agtaatgggg catgaccact ccaagggtga ggacgaaatc cgcttccgcc accagcctgt
     1321 tgatcttcag ctccgatccc gtggagaggg tgccggcgga gacgagggaa tccatgtcgt
     1381 cggcgatgtg ggatacgatc cggaaccgcc ggacggtctc ttctccgtac acctggaggt
     1441 tctgctcgtc cgtgtggggg tcgtggatgc ccgtggccac gagaagggtg accatgtcgt
     1501 cggtgatgcc cgcccgggca aagcgggcga gcaccggcgg aagcagaagg ccgtagggcg
     1561 tgggcctggt aatgtcattt actatcagga caaccttccg gggctttttc ctctcaagga
     1621 gttcgaggag gggaggcgtc cccacggggc tgtccagcgc gtcctccaca tcccgcaggg
     1681 tgcagggctt caggttcgtc tccggcggca ggaccacctg gaggatcttc gaatcctcaa
     1741 gggtgaacgc ctcggtcccc ctgcctcttt tcagctgaaa atccatctcc attccccctt
     1801 acctggtccg gagcaccgga ctcccggtct ccgcttcttc cagttcagcc acggagcggg
     1861 agacgtcggt ctccgccgcg caggcaagat cccttccaaa ccccagggcc agaatcctcc
     1921 ggccgtggac ggaccgccgg agaatagccg cc
//
LOCUS       DAOTHD010000420         1951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_69350_length_1951_cov_4.011603, whole genome shotgun sequence.
ACCESSION   DAOTHD010000420 DAOTHD010000000
VERSION     DAOTHD010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69350_length_1951_cov_4.011603"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="P5219_07520"
     CDS             complement(<1..213)
                     /locus_tag="P5219_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26644.1"
                     /translation="MEKKKRIRFFPWIAMTAVFLAFAGGAAAETVEKRFEDLVLRYGA
                     RTEARAGAAEYRAALEAGADRVSPWEL"
     gene            421..>1951
                     /locus_tag="P5219_07525"
     CDS             421..>1951
                     /locus_tag="P5219_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRX26645.1"
                     /translation="MMEENRSRQPDTVLSGILAVQPLLDSLGQELSLLDTNMRYLWVS
                     SSIAGRLGKRRENLVGRTCHSLHHSSDSPCAGCPVREALVTGETRERVIITNGKVFLS
                     RGIPVKDESGTVCAVFECERDITLEKEKEKSFLELENRYIELVENAPIGMYRVTFDGK
                     YLSANIHHARILGFPSPAVLMDEVNRTSAAVHYAEPEARKGVVDHLLANPNRWLERET
                     VFLRRDRSPIHVHLYHRSVFSPGGEPLYIDGFLEDITERKKLEEKIRSELELRQQIFD
                     AVPVHLYLKDREGKYTFVNRAFTQFRNLRHEDVIGRTASGVLEEPTASAARKEDEEVL
                     RTGGTLQDRERLIRGSGGEFWYSITKSAIFDEKGNAAGIVGCSFDITALKEISQSLTE
                     SEKRYRSLAEDMPILISTCLPDGTILYANAAYCRWFGREQQEILGTSFLDLLHPDDRV
                     ETEKNLSSLTPELPSFSSEERVFSPDGSLRWQRWINRAFFDPSGKIQFLQAVGEDITE
                     RK"
BASE COUNT          424 a          597 c          534 g          396 t
ORIGIN      
        1 gagttcccag ggggaaaccc tgtcggcccc cgcctcgaga gcggcacggt attccgccgc
       61 tccagccctg gcttcggtcc gtgctccgta ccggaggaca aggtcctcaa accgcttttc
      121 cacagtttcc gcagccgcac ctcccgcaaa ggccaggaaa acagccgtca tggcaatcca
      181 cgggaagaag cgaattctct ttttcttttc catcgctgcc gcctcctcat aattcatcag
      241 gtatgatcag tcattatacc ctcttttgta tcaggatccc atagagagat ttcccctttt
      301 cgcccaaagt ccgtaccggc gacgctcccc gccgcctacc gatttgggtt tttccgattc
      361 gtgcaggaga atgtattttc aggtagaatt tcagtacttc tcctctggaa gggtgggacc
      421 atgatggaag aaaaccgttc ccggcagccg gacacagtgc tttccggcat cctggccgtt
      481 cagcccctgc tcgattccct gggccaggaa ctctcccttc tggacacgaa catgaggtac
      541 ctctgggtat cttcgtcaat tgccgggcgc ctgggaaaac ggagggagaa tctcgtcggc
      601 aggacctgcc attcccttca ccactcatct gactccccgt gcgccggctg ccccgtccgg
      661 gaagccctcg tcaccggtga aaccagggaa agagtcatca ttaccaacgg taaagttttc
      721 ctttcccgcg gaattccggt aaaagacgag tccggaacag tatgcgctgt cttcgagtgt
      781 gaacgggaca tcaccctgga aaaagaaaag gagaagagtt tcctcgagct ggaaaaccgg
      841 tacatcgaac tggtggagaa cgcccccatc ggcatgtacc gggtcacctt cgacgggaaa
      901 tacctctcgg ccaacatcca ccacgcccgg attctcggtt tcccttcccc tgccgttctc
      961 atggatgaag tcaacaggac atcggcagcg gtccactacg ccgaacctga agcccggaaa
     1021 ggggtggtgg accatctcct cgccaatccg aaccggtggc tggagcggga gacggtcttc
     1081 ctccggaggg accgatcccc catccacgtg cacctctacc acaggagcgt cttttctccc
     1141 ggcggagagc ctttgtacat cgacggtttc ctcgaggaca tcaccgaacg gaagaagctg
     1201 gaggaaaaga tccggtccga gctggagctg aggcagcaga tcttcgacgc cgttccggtc
     1261 catctctacc tcaaggacag ggagggaaaa tataccttcg tgaacagggc cttcacgcaa
     1321 ttcaggaacc tgaggcacga ggatgttatc ggcaggacgg cttccggcgt cctggaggag
     1381 cccaccgcct cggccgcccg aaaggaggac gaggaagtgc tccgcacggg cggtaccctt
     1441 caggacaggg agcggctgat ccgcggcagt gggggagaat tttggtacag cattaccaag
     1501 agtgcaattt tcgacgaaaa gggaaacgcg gcgggcatcg tggggtgcag cttcgacatc
     1561 accgccctca aggagatttc ccagtccctc accgaaagtg agaagaggta ccgttccctt
     1621 gccgaagaca tgccgattct catttccacc tgccttcccg atggaaccat tctctacgcc
     1681 aacgccgcct actgccggtg gttcgggagg gagcagcagg agatactcgg cacctccttc
     1741 cttgatctcc ttcaccccga cgacagggtg gaaacggaaa aaaacctttc ttcccttacg
     1801 ccggaactgc cgtctttttc cagtgaagag cgggtgttct cccccgatgg ctccctgagg
     1861 tggcagcggt ggatcaacag ggctttcttc gatccctcgg ggaagatcca gttcctccag
     1921 gccgtgggtg aggacatcac cgagaggaaa a
//
LOCUS       DAOTHD010000421         1949 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_69458_length_1949_cov_2.439282, whole genome shotgun sequence.
ACCESSION   DAOTHD010000421 DAOTHD010000000
VERSION     DAOTHD010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69458_length_1949_cov_2.439282"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..90
                     /locus_tag="P5219_07530"
     CDS             <1..90
                     /locus_tag="P5219_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL14 family ribosomal protein"
                     /protein_id="HRX26646.1"
                     /translation="KGTRIFGPVGRELRAKKYMRILSLAPEVV"
     gene            107..433
                     /gene="rplX"
                     /locus_tag="P5219_07535"
     CDS             107..433
                     /gene="rplX"
                     /locus_tag="P5219_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRX26647.1"
                     /translation="MGKMRLKKGDRVRVISGKDKGKEGKVLRSIPDRDLLVIEGVNMA
                     TKHVKPSQKNPQSGIVKQEGPIYASKVMLVCPSCGAATRVGRGYLDSGKKVRLCKACG
                     EIVDQV"
     gene            449..991
                     /gene="rplE"
                     /locus_tag="P5219_07540"
     CDS             449..991
                     /gene="rplE"
                     /locus_tag="P5219_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRX26648.1"
                     /translation="MTPRFLEKYKNEVAGNLAKEFGYKNVMEIPRLKKIVVNIGAGEA
                     KVDIKYMDVSMAELTAITGQRPLLKRAKKSVAGFKVRQGMPVACTVTLRGARMWEFLD
                     RLVSLALPSIKDFQGVTRRGFDGRGNYNLGLREQLIFPEINYDKVIRPRGMNVSIVTS
                     AKTDEEAHALLRELGMPFNR"
     gene            1010..1195
                     /locus_tag="P5219_07545"
     CDS             1010..1195
                     /locus_tag="P5219_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003567540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HRX26649.1"
                     /translation="MARKAIKYKTTQPPKFAVRKYNRCPLCGRVHGYMRRFNMCRCCF
                     RKLAREGKIPGVVKSSW"
     gene            1224..1628
                     /gene="rpsH"
                     /locus_tag="P5219_07550"
     CDS             1224..1628
                     /gene="rpsH"
                     /locus_tag="P5219_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRX26650.1"
                     /translation="MYVTDPIADMLARIRNANMVFHESVDVPSSKIKMEIARILKEAG
                     YIKNYKVVSDPKKPYSSIRLFLSYGPNRERVIQGLRRISKPGRRIYVGHADLPKVMGG
                     LGIAVISTSKGLKTDTEATQLGLGGEVLCYVW"
     gene            1645..>1949
                     /gene="rplF"
                     /locus_tag="P5219_07555"
     CDS             1645..>1949
                     /gene="rplF"
                     /locus_tag="P5219_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRX26651.1"
                     /translation="MSRIGRKIIPLGSGVSIAVKDGAVTVQGPKGKLSTPTVPGIDIA
                     VEDGHIDVSRQNEQKQTKSFHGMMRALIANMVTGVTTGFKKDLEIVGVGYRAQMQGK"
BASE COUNT          492 a          531 c          584 g          342 t
ORIGIN      
        1 aagggaaccc gaatcttcgg gccggtggga cgggaactga gagcgaagaa gtacatgcgc
       61 atcctctccc tcgcccctga agttgtgtaa cggaggacaa tggactatgg gcaagatgag
      121 actgaaaaaa ggtgaccggg tccgggtcat ctccggaaaa gacaagggga aagaaggcaa
      181 ggtccttcgt tcgatccccg accgggatct cctggtgatc gagggcgtga acatggcgac
      241 gaagcatgtg aagccctccc agaagaaccc ccagtccggc attgtgaaac aggaagggcc
      301 gatctacgcc agcaaggtca tgctcgtgtg cccctcttgc ggcgctgcta cccgcgtagg
      361 ccgcggctac ctcgacagcg gaaaaaaggt ccgtctgtgc aaggcatgcg gcgaaatcgt
      421 ggaccaggtg taaggaggag agtgcgccat gacccctcgt tttctcgaga aatataaaaa
      481 cgaagtcgca ggaaaccttg cgaaagaatt cggctacaag aacgtcatgg agatcccccg
      541 cctgaagaag atcgtggtga acatcggagc gggcgaagca aaagttgaca tcaagtacat
      601 ggatgtctcc atggccgagc ttacggccat cacagggcag cgtccccttc tcaagagggc
      661 gaaaaagtcc gtggcaggct tcaaggtccg ccagggaatg cctgtcgcct gcaccgtcac
      721 cctccgggga gcccgcatgt gggaattcct cgacaggctt gtttccctcg cccttcccag
      781 catcaaggac ttccagggag taacgcggcg gggattcgac ggaaggggca actacaacct
      841 cggcctccgg gagcagctca ttttcccgga gatcaactat gacaaggtca tccgcccgcg
      901 cggaatgaac gtgtcgatcg taacgtccgc gaaaacggat gaggaagccc atgctctgct
      961 ccgcgagctg ggaatgccct tcaaccggta ggaaaggggg attttcgaga tggcaagaaa
     1021 ggcaataaag tacaagacga cgcagccccc gaagttcgcg gtgagaaagt ataacaggtg
     1081 cccgctctgc ggccgtgttc acggatacat gagacgattc aacatgtgcc gttgctgctt
     1141 cagaaagctc gctcgcgagg gaaaaatccc cggcgtagtg aagtctagct ggtagcagag
     1201 tggactgaag ggggttacga ggaatgtacg tcaccgatcc gatagcggat atgcttgcgc
     1261 gcatacggaa cgcgaacatg gttttccatg agagcgtcga tgtgccttca agcaaaatca
     1321 agatggaaat cgcccgcatt ctcaaggaag cgggctacat caagaactac aaggttgtca
     1381 gcgacccgaa aaagccctac agctccatca ggcttttcct ctcctacggt cccaacaggg
     1441 agcgggtcat tcaggggctt cgcaggatca gcaagcccgg cagaagaatt tacgtcggcc
     1501 atgccgacct tcccaaggtc atgggagggc ttggaatagc ggtcatctcc acctccaagg
     1561 gcctcaagac ggacacggaa gcgacgcagc tcggcctggg cggcgaagtt ctctgctatg
     1621 tctggtaaag tgggggtgcg ctagatgtct cgaatcggac gaaaaatcat tcccctcggc
     1681 tccggcgtga gcattgccgt caaggacggc gctgtgaccg tccagggccc caagggaaaa
     1741 ctttcgactc ccaccgttcc gggaatcgac atcgccgttg aggacgggca catcgacgtc
     1801 tccaggcaga acgagcagaa gcagaccaaa tcgtttcacg gcatgatgag ggctctcatc
     1861 gccaacatgg tcaccggtgt aaccaccggc ttcaagaaag acctcgaaat cgtcggcgtg
     1921 ggataccgcg cccagatgca ggggaaaaa
//
LOCUS       DAOTHD010000422         1945 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_69675_length_1945_cov_2.523810, whole genome shotgun sequence.
ACCESSION   DAOTHD010000422 DAOTHD010000000
VERSION     DAOTHD010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69675_length_1945_cov_2.523810"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..943
                     /gene="trpC"
                     /locus_tag="P5219_07560"
     CDS             182..943
                     /gene="trpC"
                     /locus_tag="P5219_07560"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HRX26652.1"
                     /translation="MILDRILKQKLEETAEMTRPSSRMSDALRRGGLSVIGEIKRASP
                     SRGVINGSVDPLKQLRLYEQGGADAVSILTDHSFFGGSADDFRSLRPRTALPLLRKDF
                     ILTEVQVYESLFLGADALLLIAAALERKRFRELLALAYSLGMEVIAEVHTGEELRFVL
                     DTEADIIGINNRNLDDFTVDIRTTERLMEICRQREPAGSRIIVSESGVRTEADADFLA
                     DTGVDAVLVGEALMRSGDPSGLIRAFRKKRSVRAA"
     gene            940..1575
                     /locus_tag="P5219_07565"
     CDS             940..1575
                     /locus_tag="P5219_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRX26653.1"
                     /translation="MTYVKICGMTRREDVERAVELGVSALGFILVPGSPRRLSLEAAA
                     ELASAVPPPVCRVAVMMDPGLVEVLEVERCGAFDTIQFHGSEDPSLLSGRSLGTIKAF
                     SVETGKDLEKTGRYCHAGLFLLDSRKKNLAAGKPFDHSLLAGKILARPFLLAGGLGPE
                     NLGPALSTVRPFGVDLNSGVESAPGVKDREKMAAALAAVKQFDRSERRDHQ"
     gene            1584..>1945
                     /locus_tag="P5219_07570"
     CDS             1584..>1945
                     /locus_tag="P5219_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRX26654.1"
                     /translation="MYFGPFGGQYVPETLVPALDELEKAYLASLEDPSFSAEMAPLLR
                     DYVGRPSPLYFAPRITESLGGAKVYLKREDLNHTGAHKINNALGQVLLARRMGKKRII
                     AETGAGMHGVATATAAAL"
BASE COUNT          355 a          654 c          636 g          300 t
ORIGIN      
        1 ggacgccgtg gttttcaacg ccgcggcggt ctttcttgcc gccgggcggg cgaagaactg
       61 gaaagaagga accgccctcg cggcggagag catcgactcg ggcagggcgg catcggtgct
      121 ggaaaaggtc agggcctttg cggaaccggt ccgccggtcc gcctgaaagg aggaggaccc
      181 catgattctg gaccgcatac tgaagcagaa gctcgaggaa acggcggaga tgacgaggcc
      241 gtcaagccgg atgtccgacg ccctccggcg cggcggactc tcggtcatcg gcgagatcaa
      301 gcgggcctcc cccagcaggg gggtcatcaa cgggtcggtg gatcccctga agcagctccg
      361 gctctacgag cagggggggg ccgacgccgt ctccatcctc accgaccact ccttcttcgg
      421 gggcagcgcc gacgatttcc ggtccctccg gccccggacg gcccttcctc tcctcaggaa
      481 ggacttcatc ctcactgagg tgcaggtcta cgaaagcctc ttcctcggcg ccgacgccct
      541 gctgctcatc gccgccgccc tggagaggaa gcgcttcagg gaactcctgg ccctggcgta
      601 ttccctggga atggaggtca tcgcggaagt ccacaccggc gaggaactcc ggttcgtcct
      661 ggacacggaa gcggatatca tcggcatcaa caacaggaat ctcgacgact tcaccgtgga
      721 catccgcacc acggagcgcc tcatggaaat ctgccggcag cgggagccgg ccgggagccg
      781 gatcatcgtc tccgaaagcg gcgtgcgcac cgaagcggac gccgacttcc tggccgacac
      841 cggggtggac gccgtcctgg tgggggaggc cctcatgaga tcgggtgacc cgtccgggct
      901 catccgggcc ttccggaaga aaaggagcgt ccgggcggca tgacctacgt gaagatctgc
      961 ggcatgacac ggagagagga cgtggagagg gctgtcgaac tcggcgtctc ggccctgggc
     1021 ttcatcctcg ttccggggag cccccggcgg ctctccctcg aggcggccgc ggaactggcg
     1081 tcggcggtgc ctcccccggt ctgccgggtg gccgtcatga tggaccccgg gctcgtcgaa
     1141 gtgctcgagg tggagcgctg cggcgccttc gacaccatcc agttccacgg ctccgaagac
     1201 ccttcgctgc tctcggggag atcccttggg accatcaagg ccttttccgt ggagaccgga
     1261 aaagacctgg agaaaaccgg caggtactgc catgcaggcc tcttcctgct ggacagcagg
     1321 aagaaaaacc tcgccgccgg aaagcccttc gaccactccc tcctggcggg gaaaattctt
     1381 gccaggccct ttctcctcgc ggggggactc ggcccggaga acctcggccc cgccctctcg
     1441 accgtccggc ccttcggcgt ggacctgaac agcggggtgg agtccgcccc gggagtgaaa
     1501 gaccgggaaa aaatggccgc cgcactggcc gccgtgaagc agttcgaccg ttccgaaagg
     1561 agagatcacc agtgaaaaca tccatgtact tcggcccctt cggggggcag tacgttcccg
     1621 aaaccctcgt ccccgccctg gacgaactgg agaaagccta tctcgccagc ctggaggacc
     1681 cgtccttctc cgccgagatg gcccccctgc tgcgggacta cgtgggcaga ccgtcgcccc
     1741 tgtatttcgc cccccgcatc accgaatccc tcggaggagc gaaagtgtac ctcaagcggg
     1801 aggacctgaa ccacaccggg gcccacaaga tcaacaacgc cctgggccag gtgcttctcg
     1861 cccggcggat gggcaagaag cgcatcatcg ccgagacggg tgcgggaatg cacggcgtgg
     1921 ccacggccac cgcggcggcc ctctt
//
LOCUS       DAOTHD010000423         1943 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_69770_length_1943_cov_3.283898, whole genome shotgun sequence.
ACCESSION   DAOTHD010000423 DAOTHD010000000
VERSION     DAOTHD010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69770_length_1943_cov_3.283898"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..715
                     /locus_tag="P5219_07575"
     CDS             164..715
                     /locus_tag="P5219_07575"
                     /inference="COORDINATES: protein motif:HMM:NF040334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6144 family protein"
                     /protein_id="HRX26655.1"
                     /translation="MFDVRKLQEQVIFEAVKTAGGEAAAEKVVFGDGGEAVSGDNAAW
                     VKGTMARLEREFDRETVKRIRRSCQCGYGMDERIAFAKDLVASSSSMEEFAGHERARE
                     AGLSCVDGELYLQFPFCPCPMLADVDRLETDTWCQCTTGYSKVLFECAFGCEVDVELL
                     KSVKMGDDVCLMKITPRGEVWKS"
     gene            1115..>1943
                     /gene="chvE"
                     /locus_tag="P5219_07580"
     CDS             1115..>1943
                     /gene="chvE"
                     /locus_tag="P5219_07580"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRX26656.1"
                     /translation="MKKAEEGVWIVMKKVLLALFALVLVFSSAALAADLVGVAMPTQS
                     LQRWNEDGANMKKILEEKGYKVDLQYAENNVSTQISQLENMITKGCKVLIIASIDGTV
                     LSDVLASAAAAKIPVIAYDRLIRQTPNVDYYATFDNFKVGVIQGKFIEEKLGLKEGKG
                     PFNIEFFGGSPDDNNAYFFNGGAMSVLQPYLDSGKLVCQSGQTKMEQIAILGWKSEDA
                     QARMDNLISKSYTDKKLHVVLSPNDSLAYGVAASLDNNGYIPGKDWPIITGQDCDRAN
                     "
BASE COUNT          461 a          526 c          579 g          377 t
ORIGIN      
        1 aatcgcgaat cgtcgttaga caatacaaca acaaaatctc ggattaaagg aagattttaa
       61 gactctttat tcttctctgc tcgccaaggc ctttcggggc gagctgtagg cgttccggaa
      121 aaggagaaac ggttccctta tcttgagaaa tcggggtgaa aagatgtttg atgtgcggaa
      181 actgcaggaa caggtcattt ttgaggccgt gaagaccgcc ggaggcgagg cggcggcgga
      241 gaaggtcgtt ttcggagacg gcggggaagc tgtgtccggg gacaacgccg cctgggtgaa
      301 gggaaccatg gcccgcctgg agagggagtt cgaccgggag acagtgaagc ggatccggag
      361 aagctgccag tgcgggtatg gcatggacga gcggatcgcg ttcgcgaagg acctggtggc
      421 atcttcatcc agcatggagg aatttgccgg gcatgagcga gccagggagg ccggcctgtc
      481 ctgcgtggac ggggagctgt accttcagtt tcctttctgc ccctgcccca tgcttgccga
      541 tgtggacagg ctggagacgg acacctggtg ccagtgcacc acggggtaca gcaaggtcct
      601 ttttgaatgc gccttcggct gcgaggtgga cgtggagctt ctgaagagcg tgaagatggg
      661 cgacgatgtc tgcctgatga agatcactcc ccggggtgag gtctggaaat cctgagagcc
      721 ttcacgcttt ggcagccggg aggagcccgg aagatttttc agggggcggc ctgatgtaag
      781 gatgcccggt acgctgtgcc gggaagaccg gtaggagaag aacattcggt cttgacaaaa
      841 taaaatttac gttcataata tatttattga atcaatcaat tatgataaga ggaaaagttg
      901 tcccccggaa ccttccaaac aagaatggaa aaagctgcaa attcctgttc caaatacagt
      961 tctgcccccg cgccttgcaa ccggtacgga acagggaatc ttcatttaag aggagatgat
     1021 gccgctcccg ctgcagacaa cagggctccc ctgccgaaag gggatgcgga acgaaggcgc
     1081 ctcccccggg gaggatgaaa aggggcagcg ccccttgaag aaagctgaag aaggagtgtg
     1141 gatcgtcatg aagaaagtgc ttctcgctct tttcgctctt gtcctggtct tctcctccgc
     1201 cgcacttgca gcagatctcg tgggcgtggc catgcccacc cagtccctcc agcgctggaa
     1261 cgaagacggc gcgaacatga agaagatcct cgaggaaaag ggatacaagg tggatcttca
     1321 gtatgccgag aacaacgtga gcacccagat ttcccagctt gaaaacatga tcaccaaggg
     1381 atgcaaggtc ctcatcatcg cctccatcga cgggaccgtc ctctccgacg tcctggccag
     1441 cgccgcggcg gccaagatcc ccgtcatcgc ctacgaccgt ctgatccgcc agactcccaa
     1501 cgtggactac tacgccacct tcgacaactt caaggttggc gtcatccagg gaaaattcat
     1561 cgaggaaaag ctcggtctga aggagggcaa ggggcccttc aacatcgagt tcttcggcgg
     1621 ttcccccgac gacaacaacg cctatttctt caacggcggt gccatgagcg tgctgcagcc
     1681 ctacctcgac agcggcaagc tggtgtgcca gtccggccag acgaagatgg agcagatcgc
     1741 cattctggga tggaagtccg aggatgccca ggcccggatg gataacctga tctccaagtc
     1801 ctacaccgac aagaagctcc acgtagtgct ttcccccaac gactccctcg cttacggtgt
     1861 cgccgcttcc ctcgacaaca acggctacat tcccggcaag gactggccta tcatcaccgg
     1921 acaggactgc gacagggcca acg
//
LOCUS       DAOTHD010000424         1939 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_69966_length_1939_cov_3.715499, whole genome shotgun sequence.
ACCESSION   DAOTHD010000424 DAOTHD010000000
VERSION     DAOTHD010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69966_length_1939_cov_3.715499"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="P5219_07585"
     CDS             complement(<1..780)
                     /locus_tag="P5219_07585"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX26657.1"
                     /translation="MGKMVKWLSALFVVVLFTAAAWGAGPEDFFKAGMKAANADDYER
                     AAAAFRRAISMKEDYAEAHYNLGVALSGLKRFREASASYRRTTELKPSYAQAWNNLGV
                     ALKELEQFEEAEAAFRKAAETDGKYWAPHYNLGDLYAALGKNDRAIESLKHAANLSPR
                     SVEPRLKLGAVYASLKQYGNALSQFEQARDIRQDIPEIHYSEGICHAGLQNQEKAAGA
                     FRRAVKLRPVYPLAWYNLGVSLFSLNRQEEAAEAFEKAAEQA"
     gene            complement(814..>1939)
                     /locus_tag="P5219_07590"
     CDS             complement(814..>1939)
                     /locus_tag="P5219_07590"
                     /inference="COORDINATES: protein motif:HMM:NF013717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HRX26658.1"
                     /translation="ALLSAAGMTRDGAEEVYSWLLRFSPVTQYPAFGAQRYLHGDGQF
                     RWIGAVAVLLGAFADKGAGLAFSKDEMALFPERLLPAPARTRTLSVSGWPLELDGLEP
                     PVEVLVVSAADPLRQNPASDLVRRAYEKIPFTVCADFVLSDTAKASDLVLPITTFLEE
                     EGDWKGSYWHSYLVRSERVLPPRPGTLDETEIYTRLADRLGLSCDLVAMKKEMDRDLM
                     ESPLLEALGDGVYRWNEPPCRLDGGRKALLPTEVPGISRGPEGYLRLVTVHRKEYING
                     QSWDAPCAAGLPVLLLNPADARRRGVKDGEEVLARNARGGTLSVRVAEDPSLGAGYCI
                     LPQGTKGVNLLTDPLVSPGWGAPFAESWIDLYPGKDRPEK"
BASE COUNT          336 a          608 c          609 g          386 t
ORIGIN      
        1 agcctgctcc gccgcctttt cgaaggcctc cgccgcctct tcctgccggt tgaggctgaa
       61 aagggatacc cccaggttgt accacgccag ggggtagacg ggtcggagct tcacggcccg
      121 ccggaaggcc ccggctgcct tttcctggtt ctgcagcccg gcgtggcaaa tgccttcgga
      181 ataatggatt tcaggaatgt cctgccggat gtcccgggcc tgctcgaact gggaaagggc
      241 gttcccgtac tgcttcagcg aggcgtagac cgcaccgagc ttcagccggg gttccacgga
      301 acggggtgac aggttcgcgg cgtgcttgag ggattcgatt gcccggtcgt tttttccgag
      361 ggcggcatac agatctccaa gattgtagtg gggcgcccag tatttcccgt ctgtttcagc
      421 ggctttccgg aaggccgcct cggcttcctc gaactgctcc agctctttaa gggctacacc
      481 aagattgttc catgcctggg cgtaggaggg ttttagttcc gtggtcctcc tgtaggatgc
      541 ggacgcctcc ctgaaccgct tcaggcccga gagagcgact cccaagttgt agtgggcctc
      601 cgcgtaatct tccttcatcg agatcgctct gcggaatgcg gcggcagccc gttcatagtc
      661 atccgcattt gcggccttca ttccggcttt gaaaaagtcc tccggtcctg ctccccatgc
      721 cgctgccgtg aaaaggacga cgacaaaaag ggcggatagc catttcacca tttttcccat
      781 ggggtcaact cctttctttt ctttgtgcgg tctttatttt tccggtctat cctttccggg
      841 ataaagatca atccagctct cggcaaaggg cgcaccccac cccgggctga ccaggggatc
      901 ggtgagcagg ttcacgccct tcgtgccctg gggaagaatg cagtatcccg cgcccaggga
      961 agggtcttcc gccacccgga cggacagggt gcctccccgg gcgttgcggg ccaggacttc
     1021 ctctccgtcc ttcactcccc gcctccgggc gtcggcggga ttcagtaaga ggacgggaag
     1081 gccggccgcg cagggagcgt cccagctctg gccgttgatg tactccttcc tgtggaccgt
     1141 caccagccgg agataccctt ccggcccccg ggagattccg ggaacctccg tgggaagcag
     1201 ggccttccgg cccccgtcca gccggcaggg aggttcgttc cagcggtata ctccgtcgcc
     1261 gagggcttcg agcaggggcg attccatgag gtccctgtcc atttccttct tcatggcaac
     1321 cagatcgcag gaaaggccaa gcctgtccgc aagacgggta tagatttccg tctcgtcaag
     1381 ggttccggga cggggaggaa gaacccgctc gctgcgtacg aggtagctgt gccagtagga
     1441 gcccttccag tctccctctt cctcgaggaa ggtggtaatg ggcagcacca ggtccgaggc
     1501 cttcgccgta tctgaaagga caaaatcggc gcagaccgtg aaggggattt tctcgtaggc
     1561 cctgcggacg aggtccgagg cgggattctg ccgcagcggg tcggcggcgg acaccacgag
     1621 cacctccacg ggcggctcca ggccgtcaag ttccaggggc caccccgaaa cggagagggt
     1681 cctggtcctc gccggagctg gaaggagccg ttccgggaag agagccattt cgtccttgct
     1741 gaaggcaagc cccgctccct tgtccgcgaa ggcgccgaga agaaccgcca cggccccgat
     1801 ccaccggaac tggccgtcgc cgtggaggta ccgctgggct ccaaaggcgg ggtactgggt
     1861 gacgggtgaa aagcgaagaa gccaggaata tacctcttcc gctccgtccc gggtcatccc
     1921 tgccgccgag aggagggcg
//
LOCUS       DAOTHD010000425         1938 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_70021_length_1938_cov_3.351036, whole genome shotgun sequence.
ACCESSION   DAOTHD010000425 DAOTHD010000000
VERSION     DAOTHD010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70021_length_1938_cov_3.351036"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..219)
                     /locus_tag="P5219_07595"
     CDS             complement(94..219)
                     /locus_tag="P5219_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26659.1"
                     /translation="MEKTTHELKTAKSASRDDSSDTFNNLMSDLNHDAYKSRYDQ"
     gene            379..1263
                     /locus_tag="P5219_07600"
     CDS             379..1263
                     /locus_tag="P5219_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRX26660.1"
                     /translation="METVKLGIATEYIPGRVNVGLYVSDNGARLIDSGLDDEAGRKIA
                     RMLEERKIPLQRIVTTHSNADHCGGNAFLRKRTGCSVAATKLESVVIENPFLEPLVLW
                     SAFPFGELENKFLQAKPSEVDLIIENSGIVDESGLEAVPLPGHFLDMIGVRTPDDVLF
                     VADSVFSEELIEKYAVMFVLDVGAALETLDRLIRAEAAWFVPSHAPASENIVPLAEAN
                     RKGLLRVSEAVLESCGEPASREKILQGIAGRFSLSMSVSEYVLNSAAVGAHLSWLRKR
                     GQVAPVVEKGVLLWRKTR"
     gene            complement(1271..>1938)
                     /locus_tag="P5219_07605"
     CDS             complement(1271..>1938)
                     /locus_tag="P5219_07605"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRX26661.1"
                     /translation="EERHRLAISALVSMRSNQVFMGIPAVSIALGSRGLESLSLYFAM
                     SLVGYHMISIAASQIVLSGGISPRSILNSARKLATNPMVIACFAGIGFSLAGVNEFPR
                     PVDVTLKVLGDIGTGMALLAVGAGLSFRSVPSMLRKTWRDALIKLVVQPAVVWAFFLA
                     WPVDSLMVKVVVFVCAMPVAVNSLVVAQGMGMDDQYAGEVIAVTTVFSAVTLPFWIRV
                     LGI"
BASE COUNT          490 a          486 c          559 g          403 t
ORIGIN      
        1 tttgacactc ccgtcacctc ctgtttaact gaaacattat aacgcaaccg gagggaacgt
       61 caaagaaaaa accgcccgtg agggcggctg ttcctactgg tcgtaccggg acttataggc
      121 gtcgtgattc aaatcggaca tcaggttgtt gaaagtatcg gacgaatcat cccgggaagc
      181 gcttttggcg gttttcagtt catgcgttgt cttttccatg atacatctcc tttgctgcag
      241 gataaagaat tcctcaaatt ggtccgtacc aaatatgaaa aaattatagc acatggtccg
      301 gactgtgcaa ggggtattgc caacattttt tgtttgattc acaataacgg aaggaatcaa
      361 aagaaggggg acgggcttgt ggagacggta aaactgggta ttgcaaccga gtacattccg
      421 ggaagagtca acgtgggact gtatgtatca gacaacggtg cccggctgat cgacagcggc
      481 ctggacgacg aagcgggaag aaaaattgcc cgcatgctcg aagaaagaaa aattcctctc
      541 caaagaatcg taacgaccca ttccaatgcg gatcactgcg gaggaaacgc gttcctgaga
      601 aagaggaccg gctgctcggt tgcggcgacg aaactcgaat cggtggtcat tgaaaacccg
      661 ttcctggaac ctcttgttct ctggtcggcc ttccccttcg gggaacttga aaacaagttt
      721 cttcaggcga agccgtcgga agttgacctg atcatagaaa atagtggaat agtggacgaa
      781 tccggactgg aagcggtccc tctgccggga cattttctcg acatgatagg cgtccggact
      841 ccggatgacg tgctgttcgt ggcggattcg gtcttttcgg aagaactcat tgaaaaatat
      901 gccgtcatgt ttgtccttga cgttggggcg gccctggaaa ccctggaccg tctgatcagg
      961 gctgaagccg cctggtttgt tcccagccac gcgcctgcct cggaaaatat tgttcctctc
     1021 gcggaggcaa accggaaggg gcttcttcgg gtgagcgagg cagtcctgga aagctgcggg
     1081 gagccggcaa gcagggagaa aatccttcag ggcattgccg gcaggttctc tctttccatg
     1141 tcggtgagtg aatacgttct caacagcgcg gccgtgggag ctcatctctc atggctgaga
     1201 aaacggggac aggtggctcc cgttgtggag aagggagtat tgctctggcg aaaaacgaga
     1261 tgaaaagggg ctaaattccg agaaccctga tccagaaagg aagggtgaca gccgaaaaga
     1321 cggttgttac cgcaatcact tctcccgcat actggtcgtc cattcccatt ccctgggcaa
     1381 ccacgagaga gttcaccgcc acaggcatgg cgcatacaaa gaccacaacc ttaaccataa
     1441 gggagtccac cggccatgcg agaaagaacg cccatacaac tgcaggctga acaacgagtt
     1501 ttatcagcgc atctctccag gttttcctga gcatggaggg aacggaccgg aaagaaagcc
     1561 ccgctcccac ggcgaggagc gccatgcccg tcccgatgtc gcccagtact ttcagggtaa
     1621 catccaccgg gcggggaaat tcgttcaccc ctgccagaga aaagccgatt cctgcgaagc
     1681 aggcgatcac catgggattg gtggcgagtt tcctggccga attgagaatg gacctcggcg
     1741 agattccccc ggagaggaca atctgggagg cggcgatgga aatcatgtgg tagcccacaa
     1801 ggctcatggc gaaatacagg gagaggctct cgaggcctct tgaacccagg gctattgaaa
     1861 cagcggggat tcccatgaag acctggttcg agcgcatgga aacgagcgcg gaaatggcga
     1921 ggcggtgccg ttcctcac
//
LOCUS       DAOTHD010000426         1930 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_70415_length_1930_cov_3.501867, whole genome shotgun sequence.
ACCESSION   DAOTHD010000426 DAOTHD010000000
VERSION     DAOTHD010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70415_length_1930_cov_3.501867"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1144
                     /locus_tag="P5219_07610"
     CDS             <1..1144
                     /locus_tag="P5219_07610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HRX26662.1"
                     /translation="KILVPEGKDVPVGELLAYLGQSGEAVPGASAPEPAGAVPAQAVP
                     AEGAEKPSSRSAALPAGLRATPLARKIARDEGIDLSSVPGSGPEGRIVRKDVESFAAS
                     APETRKVKTSPAAAKAAADLGVDVSSIAADGRVMKADVLKAASSAARPAPQDIRLPLT
                     PMRKVIAERMTLSRATIPSVAYDMDVDFTALAEFRAKVKAEGAKRGVKISYNHILMKI
                     CAAAVKDVPLANSSFDGDAILLHGNVNIGLAVAVDGGLLVPNVKAVQEKSLLEIAEET
                     ERLVEQARSGKLALEDMQGGTFTITNLGMFGTKSFTPIVNPPEACILGVNTITEKPVV
                     VDGAVVIRPMSVLSLVADHRILDGAEAARFLARVRELAENPWLLLL"
     gene            1232..>1930
                     /locus_tag="P5219_07615"
     CDS             1232..>1930
                     /locus_tag="P5219_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRX26663.1"
                     /translation="MAVVTMPKLGLTMNEGTVSRWNKKVGDQVEKGEILLVVATDKLT
                     FEVEAAESGVLSEILVGEGEAAAVSAPIARIGEGGAEEAPASDPHAPAEEKPLQKAAP
                     ATVQSTPAAAGTKKVVVIGGGPGGYVAAIRAAQLGGEVTLIEKGALGGVCLNVGCIPT
                     KVLLHTAEAYTAAKEGALIGLRAENVSVDWSGLMARKKAVVDTLVGGVGTLMASNGIT
                     VLRGTASFASPKEID"
BASE COUNT          366 a          586 c          662 g          316 t
ORIGIN      
        1 gaagatcctt gttcctgaag gaaaggacgt tccggtaggg gagcttctcg cctacctcgg
       61 acagtccggt gaggccgttc ccggagcgtc cgcacctgag ccggccgggg ccgtcccggc
      121 tcaggccgtt cctgcggagg gggcggaaaa gccttcttcc cggagcgccg ccctgcccgc
      181 ggggctgagg gcgacacccc tggcacggaa gatcgccagg gacgagggga ttgacctttc
      241 atccgtgccg ggatcgggac cggagggcag gatcgtccgg aaggacgtgg aatccttcgc
      301 ggcctccgcc ccggagacca gaaaggtcaa gaccagcccg gcggcagcga aagccgctgc
      361 ggatcttgga gtggacgttt cgtcgattgc agcggacggc cgggttatga aggccgacgt
      421 gctcaaggcc gcgtcttccg ccgcaaggcc ggctccacag gatatccgcc tgcccctgac
      481 ccccatgcgg aaggtcatcg ccgagcgcat gaccctgagc agggccacca ttccctcggt
      541 ggcctacgac atggacgtgg acttcaccgc cctcgcggag ttccgggcga aggtgaaggc
      601 cgaaggggcg aagcggggcg tcaaaatctc ctacaaccat atcctcatga agatctgcgc
      661 cgcggccgtg aaggatgtcc ccctggcgaa ctcctccttt gacggcgacg ccattcttct
      721 tcacggaaac gtgaacatcg gccttgccgt cgctgtggac ggagggctcc tcgttcccaa
      781 cgtgaaggcc gtccaggaaa agagtcttct tgaaatagcg gaggagactg aacggctggt
      841 tgaacaggca cggtccggaa aactggccct tgaggacatg cagggcggga ctttcaccat
      901 cacgaacctc ggcatgttcg ggacgaagag cttcaccccc atcgtcaacc cgccggaggc
      961 gtgcatcctc ggcgtgaaca ccattacgga aaagcccgtt gtggtggacg gagcagtggt
     1021 catccggccc atgtcggtgc tctccctggt ggcggaccac cggattcttg acggggccga
     1081 agctgcccgt ttcctcgcca gggtccggga actggcggaa aacccctggc ttcttcttct
     1141 gtaggccggg agaacggaaa tgcggattgc ccggcgggag acattcggac cccgccgggt
     1201 tttttattga acagaacggg aggttgtgga aatggctgtt gtgacgatgc ccaagctggg
     1261 gctgacgatg aacgaaggaa cggtctcacg gtggaacaaa aaggtggggg accaggtgga
     1321 gaagggagaa atccttctcg tggtggcgac ggacaagctg accttcgagg tggaggccgc
     1381 cgagtccggt gtgctgtccg agatactggt cggggaaggg gaagccgccg ccgtgtctgc
     1441 tcccatagcc cggatcgggg agggcggggc tgaagaggcc ccggcatccg atccccacgc
     1501 ccccgctgag gaaaagcctc ttcagaaggc ggctccggcc actgtgcagt caacccccgc
     1561 tgctgccgga acgaagaaag tagtggtcat cggcggcggc ccgggagggt acgtggctgc
     1621 catccgggcg gcccagctcg gaggggaggt caccctcatc gagaaaggcg ccctcggcgg
     1681 cgtctgcctc aacgtggggt gcatccccac caaggtgctc ctccacacgg cggaagccta
     1741 taccgcggcg aaggaaggag ccctcatcgg gctccgggcg gagaacgtct ccgtggactg
     1801 gagcggtctc atggcgcgga agaaggccgt ggtggacacc cttgtgggcg gcgtgggcac
     1861 ccttatggcc agcaacggga tcactgtcct ccggggaacg gcctccttcg cctctccgaa
     1921 ggaaatcgac
//
LOCUS       DAOTHD010000427         1929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_70494_length_1929_cov_2.222519, whole genome shotgun sequence.
ACCESSION   DAOTHD010000427 DAOTHD010000000
VERSION     DAOTHD010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70494_length_1929_cov_2.222519"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1525)
                     /gene="carB"
                     /locus_tag="P5219_07620"
     CDS             complement(<1..1525)
                     /gene="carB"
                     /locus_tag="P5219_07620"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRX26664.1"
                     /translation="MPARRDLKKILLPGSGAIVIGQACEFDYSGVQAVKALKEEGYEV
                     VLVNPNPATVMTSPGIADRVYLEPLKVPYLEEIIKTERPDAILPTMGGQTALNLVLEL
                     ADEGILDRWGVEVIGASVESIRLAEDRGRFKRAMEDIGLDVPKSVLARSVQEAEAFAS
                     ETGLPVVIRPSFTLGGVGGAVASTKEELRARVERALLESPVKSALVEESLLGWKEFEM
                     EVMRDRKDNAVVVCSIENIDPMGVHTGDSITVAPVQTLSDREYQAMRSAAIDVLRAVG
                     VDCGGSNVQFAVNPGSGRMTVIEMNPRVSRSSAMASKATGFPIARAAAKLAVGYTLDE
                     ISNEITGSTTGCFEPALDYVAVKFPRFETDKFPAEYDRLGTQMKSVGEALSLGRTFME
                     ALNKAARASEQGYGGIHELPMGYDGLDEMLTTPHPKKIFAAYTVLKREGRRALRDLAE
                     KTAYDPWFLSQMADQAEFEDRIAGEPLTEDLLLKAKRFGLSDRRIARRSCTHSTSRCS
                     "
     gene            complement(1504..>1929)
                     /locus_tag="P5219_07625"
     CDS             complement(1504..>1929)
                     /locus_tag="P5219_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRX26665.1"
                     /translation="SLAEVIRELLGRLPVFGICLGHQLIAHALGGTTQKMKFGHHGCN
                     HPVRDERTGQVQVTSQNHGFEVDGSSLPPGVNVWFRNANDGTVEGLYHECLPVLSCQF
                     HPEGAPGPRDSLWVFDEFVKAAAAFSAEKEMIPCRHAGI"
BASE COUNT          335 a          637 c          587 g          370 t
ORIGIN      
        1 ccgagcagcg cgaggtgctg tgggtgcagg atcggcgggc gatccgcctg tccgaaagac
       61 cgaagcgctt cgccttcagg aggagatcct ccgtgagcgg ttccccggcg atccggtcct
      121 cgaattccgc ctggtctgcc atctgggaga gaaaccaggg atcgtaggcc gttttttccg
      181 caaggtcccg gagagcccgg cggccctccc gcttcagtac cgtgtaggca gcgaaaattt
      241 tcttcgggtg gggagtggtc agcatttcat cgaggccgtc gtaccccatg ggaagttcgt
      301 ggattccccc gtacccctgt tcagaggccc gggccgcctt gttcagcgcc tccatgaagg
      361 tcctgcccag ggaaagagcc tcaccgactg atttcatctg ggttccgaga cggtcatatt
      421 ccgccgggaa cttgtccgtc tcgaacctcg gaaacttcac ggcaacgtaa tccagggcgg
      481 gctcgaagca gccggtagtg ctgccggtga tctcgtttga aatctcgtcg agggtatagc
      541 ccacagccag cttggccgca gcccgggcta tgggaaatcc cgtagccttg gaggccatgg
      601 ccgacgagcg gctcacccgg gggttcatct cgatcaccgt catccttccc gaaccgggat
      661 tcacggcgaa ctggacgttg gaacctccgc agtccacccc caccgcccgg agcacatcga
      721 tggcggctga cctcatcgcc tggtattccc tgtcgctcag ggtctgcacg ggggctacgg
      781 tgatgctgtc cccggtgtgg actcccatgg gatcgatatt ctcgatggag cagaccacca
      841 cggcgttgtc cttcctgtcc cgcatgacct ccatctcgaa ttccttccat cccaggagag
      901 attcctccac gagagccgac ttgaccgggc tttcaagcag ggcccgctcg acccgggccc
      961 tgagctcctc tttcgttgag gcgacagccc cacccacacc cccgagggtg aaggaggggc
     1021 ggatgaccac tggcagaccg gtttccgagg cgaacgcctc ggcttcctgt acagatcggg
     1081 caaggactga cttcgggaca tcaaggccga tatcctccat ggccctcttg aaccgccccc
     1141 ggtcctccgc cagccggatg gactccaccg acgcgccgat gacctccacg ccccaccggt
     1201 ccagaatgcc ttcgtccgcc agttccagca ccaggttcag ggctgtctgc cctcccatgg
     1261 tgggcaaaat ggcgtcgggg cgctccgttt tgattatctc ctccaggtag ggaaccttga
     1321 gaggttcgag gtacactctg tccgcaatgc cgggcgaagt catcaccgtc gcgggattgg
     1381 ggttcacgag caccacctcg tacccttcct ccttcagggc cttgacggcc tggacaccgg
     1441 agtagtcgaa ttcgcaggcc tggccgatga cgatggctcc cgatccggga aggaggattt
     1501 tcttcagatc cctgcgtgcc ggcatggaat catctccttt tctgcggaaa aggcggcggc
     1561 ggccttcacg aactcgtcga agacccacag ggagtcccgg ggaccgggcg cgccctccgg
     1621 gtgaaactgg caggaaagca ccggaaggca ttcgtggtac agtccctcca ccgtgccgtc
     1681 attggcgttc cggaaccaga cgttcacccc ggggggaagg gaggagccgt ccacctcgaa
     1741 accgtggttc tggctggtca cctggacctg ccccgtccgc tcgtcccgga cggggtggtt
     1801 gcacccgtgg tggccgaact tcatcttctg tgtcgttcct cccagggcgt gggcgatcag
     1861 ctggtgcccc aggcagatgc cgaacaccgg aaggcggccc aggagctccc ggatcacctc
     1921 cgccaggga
//
LOCUS       DAOTHD010000428         1927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_70589_length_1927_cov_3.011218, whole genome shotgun sequence.
ACCESSION   DAOTHD010000428 DAOTHD010000000
VERSION     DAOTHD010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70589_length_1927_cov_3.011218"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="P5219_07630"
     CDS             complement(<1..549)
                     /locus_tag="P5219_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HRX26666.1"
                     /translation="MPVGFRVFLQRNLPPKDLVESFRTIPAANIADCMGRHNAMIPEI
                     RLMTKPGEVNMVGVALTVKTCYGDNLMIHQGLDMAAEGDVLVIANEEGRNRSLLGEIM
                     YRYAERKNLAGIVLDGPIRDALQISGGSVPLYATGSTPGGPYKEGPGEVNVPVSCGGV
                     HVNPGDIIVGDPDGVIVIPRQDA"
     gene            complement(585..1475)
                     /locus_tag="P5219_07635"
     CDS             complement(585..1475)
                     /locus_tag="P5219_07635"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HRX26667.1"
                     /translation="MAVTLGFLGFGEVGYYMAKGLKTAGFGRIVAFDKAAADGGPFAR
                     TIGSRAEEAGVELVSSLQDMLGLAEVVVSALPAKYSLSAAREAAGYTTAARLFVDVST
                     AKPSEKREMEGLFAGKGILFADGAMLGPLPTYGHTVPILASGSGAAQWAEMMTPFGMK
                     IDLAEGPAGAASSIKLVRSVFMKGLEALLVETFLYAKKSGAEEIVLESLAETLNVPFQ
                     NTARRMIAADLVHAERRAFEVGESVELMKDLGIEPIMAEAVIRRLKKSAALGTREELG
                     GVPPKSLPEVYEIWRTKGHC"
     gene            complement(1500..>1927)
                     /locus_tag="P5219_07640"
     CDS             complement(1500..>1927)
                     /locus_tag="P5219_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HRX26668.1"
                     /translation="RTDAIAVSGVDEAIRRGNAYIEAGADMIFVEAPRTVEEIEKAVK
                     GIKGLVSINLFDNIEGGKTPLLSVEELAAMGVARISIPVGTTFAAARGVLNYLEAIKG
                     GRLAPERKDLVFSFNEFKDLVGVPEFRDQEKEFLPTFVE"
BASE COUNT          361 a          604 c          548 g          414 t
ORIGIN      
        1 tgcgtcctgc cgcggaatga cgatgacgcc gtcaggatct ccgacgatga tgtcaccggg
       61 gttgacgtgc acaccgccgc aggagacggg aacgttcacc tcgcccggtc cctccttgta
      121 gggaccgccc ggtgtcgaac cggtggcgta gagggggacg gaaccgccgg agatctggag
      181 ggcatcccgg ataggaccgt cgaggacgat tccggcaagg ttctttctct cggcgtaccg
      241 gtacattatt tccccaagga gggacctgtt tcttccctct tcgttggcaa tcaccaggac
      301 atccccttcg gccgccatgt cgagcccctg gtggatcatc aggttatccc catagcaggt
      361 tttcaccgtc agggcgacac ccaccatgtt gacctcgccg ggcttcgtca tcagccggat
      421 ctcggggatc atggcgttgt gtcgtcccat gcagtcggct atgtttgccg ccgggatcgt
      481 ccggaagctt tcgacaagat ctttcggggg aaggtttctt tgaagaaaaa cacggaatcc
      541 aacgggcatt gttttctctc cttaaggggt cggtttgcgt tttatcagca gtggcctttt
      601 gttcgccaga tctcatagac ctcgggaagg ctcttcggcg gcaccccgcc gagctcctcc
      661 ctcgttccga gggctgccga ctttttgagg cgccggatga cggcttccgc catgatgggc
      721 tcgataccca ggtccttcat gagttccacc gactctccca cttcgaaggc cctgcgttcg
      781 gcatggacaa ggtcggcggc gatcattctc cgggcggtgt tctggaaggg aacgttcagg
      841 gtttccgcca gggattccag cacgatctcc tccgcgccgc tctttttggc gtacaggaac
      901 gtctccacga gaagagcctc aagacctttc ataaagacgc tgcggacaag cttgatggat
      961 gaggccgccc cggcggggcc ttcggcgagg tcgatcttca tcccgaaggg agtcatcatc
     1021 tcagcccatt gggcggctcc gctccccgaa gcgagaatgg gaaccgtgtg gccgtaggtg
     1081 ggaagagggc cgagcatggc cccgtcggca aacaggatac cctttcctgc aaaaagcccc
     1141 tccatctccc tcttttccga gggttttgcc gtggacacat cgacgaaaag ccgtgcagcc
     1201 gtcgtatatc ctgccgcctc cctggccgcg gaaagggagt acttcgccgg aagggcggag
     1261 acgaccactt cagccagacc gagcatatcc tgaagtgaag aaacaagttc cactcccgct
     1321 tcttcggctc tcgaaccgat ggtccgggca aacggacctc cgtcggccgc agccttgtcg
     1381 aaagccacaa tcctgccgaa gcctgccgtt ttcagcccct tcgccatgta gtagcccact
     1441 tctccgaagc cgagaaaacc aagagtaaca gccatctgtc atctcccctt tcggaatttc
     1501 tactcgacga aggtagggag gaattccttc tcctgatccc tgaattccgg cacccccacg
     1561 aggtctttga attcattgaa ggagaagacc agatcctttc tctcgggagc aaggcggcct
     1621 cccttgatgg cctcaaggta attgagcact ccccgggctg ccgcgaaggt cgtccccacc
     1681 ggaatgctga tgcgggcgac tcccatggcc gccagttctt ccacggacag gaggggtgtc
     1741 tttccgccct cgatattgtc aaagaggttg atgctcacca gccccttgat acccttgacg
     1801 gccttctcga tctcctccac cgtgcgggga gcctcgacga agatcatgtc cgccccggct
     1861 tcgatatagg cgttgcccct gcggatggcc tcgtcgacac cggatacggc gatggcgtca
     1921 gtcctgg
//
LOCUS       DAOTHD010000429         1920 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_70938_length_1920_cov_3.856836, whole genome shotgun sequence.
ACCESSION   DAOTHD010000429 DAOTHD010000000
VERSION     DAOTHD010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70938_length_1920_cov_3.856836"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="P5219_07645"
     CDS             <1..552
                     /locus_tag="P5219_07645"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRX26669.1"
                     /translation="MIEQMLRDLAASDPEWSIALLRYFNPIGAHQSGLIGEDPRGIPN
                     NLFPYVCRVASGRLEKLSVFGDDYPTPDGTGVRDYIHVSDLAEGHVSALKFLEGMGGA
                     EAFNLGTGKGTSVLELIRAFERASGKAVPFEITGRRPGDIAVCYARTDRARKMLGWSA
                     KRTLDDMCRDGWNRELLRDDGEK"
     gene            complement(604..1074)
                     /locus_tag="P5219_07650"
     CDS             complement(604..1074)
                     /locus_tag="P5219_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRX26670.1"
                     /translation="MTVAFDMKEALRYGIHAEIEAKRFYKMWADSVDEEHLKKELLEL
                     SDWESEHEASLLNYYEKMYGEKCPIDPDLVVAPELKVQTKDFGDVTSVLRIASAAYLS
                     EMRAMEFYERLEKESKGETAMMFGKIKDMEKEHMDITKKRYLKIREDVVGFRAF"
     gene            1252..>1920
                     /locus_tag="P5219_07655"
     CDS             1252..>1920
                     /locus_tag="P5219_07655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRX26671.1"
                     /translation="MDLWKSVREIEEDVVAYRRHIHENPELSLKEFDTADYVETVLKG
                     IEGVDSIRRISPTGILAEVRGTKEGKGRCIALRGDMDALPGDEMADVPFKSKRPGVVH
                     SCGHDVHTALLLGAVRILARHRGEFAGTVKFFFQPAEEVLGGAIQFLGAGALENPKVD
                     AAVGIHVFSDHDAGIISSRKGPMLAGADGLTIGVKGCQSHAAHPHTGRDAVVIAGQVI
                     GALQT"
BASE COUNT          372 a          579 c          591 g          378 t
ORIGIN      
        1 atgatcgagc agatgcttcg tgacctggcg gcctcggacc cggaatggtc cattgccctc
       61 ctgcggtact tcaatcccat cggggcccac caaagcggcc tgatcggcga ggatcccagg
      121 ggcatcccga acaacctctt cccctatgtc tgccgggtgg cctcaggtcg cctggaaaag
      181 ctctccgttt tcggcgacga ctatccgacc cctgacggca cgggagtccg tgactatatc
      241 catgtttccg acctggccga ggggcacgtg agcgccctca aatttctcga gggaatgggt
      301 ggtgcggagg ctttcaacct gggaaccgga aaaggaacgt cggtgctcga gctgatccgg
      361 gccttcgaga gagcctcggg gaaggccgtt cccttcgaga tcaccgggcg gcgtcccggg
      421 gacatcgccg tctgctatgc ccgcacggac agggcccgga agatgctcgg ctggtcggcg
      481 aagcgcaccc tggacgacat gtgccgcgac ggatggaacc gggaactcct gagggatgac
      541 ggagaaaaat aacaggacgc ccccggacgg gggcatcctg ctttgaatcg aagccccaag
      601 gaatcagaag gccctgaagc ccaccacgtc ctcgcggatc ttgaggtagc gtttcttggt
      661 gatgtccata tgctccttct ccatgtcctt gatcttgccg aacatcatgg ccgtctcgcc
      721 cttgctctcc ttttcaaggc gctcgtagaa ttccatggcg cgcatctcgg agagataggc
      781 ggcggaggcg atgcggagca cggaagtgac gtcgccgaag tcctttgtct ggaccttcag
      841 ctcgggggcc accaccaggt cggggtcgat ggggcatttc tcgccgtaca tcttctcgta
      901 gtagttcagg aggcttgcct cgtgctcgct ctcccagtcg gagagctcca gcagctcctt
      961 cttgaggtgt tcctcatcca cgctgtcggc ccacatcttg tagaaccgct ttgcctcgat
     1021 ctctgcatgg atgccgtacc ggagagcttc cttcatgtcg aatgccactg tcatattcca
     1081 tcccctcctg atatatgggt tatttccgct cttcattata ccctataccc gagaaaattc
     1141 caaaaataga aattgactac aaaccggaga cgtctccctg cctttcaaaa gaattccccc
     1201 tgtgagagaa tgaaaacaga atgcatttcc gatgaaaggc tgggatagac aatggattta
     1261 tggaaatcgg tccgggaaat tgaagaggac gtggtggcgt accgccgtca catccacgag
     1321 aatcccgaac tgagcctgaa ggagttcgac accgccgact acgtggagac ggtgctcaag
     1381 gggattgaag gggttgattc gatccgccgg atctccccca cgggaattct cgccgaagtg
     1441 cgggggacga aggaggggaa gggccggtgc attgccctga ggggggacat ggacgccctt
     1501 cccggcgacg aaatggctga cgtgcctttc aagtccaaac ggccgggggt ggtccattcc
     1561 tgcggccacg acgtccacac cgccctgctg ctgggcgcgg tgcggattct ggcccgccac
     1621 aggggcgagt tcgccgggac ggtgaagttc ttcttccagc ccgccgagga ggtcctcggg
     1681 ggagcgatcc agttcctcgg ggccggggcc ctggagaacc cgaaggtcga tgccgccgtg
     1741 ggcatccacg tgttctccga ccatgacgcg ggcattatct cctccaggaa ggggcccatg
     1801 ctcgccgggg ccgacggcct gaccatcggg gtgaagggat gccagtccca cgcggcccat
     1861 ccccacacgg ggcgggatgc cgtggtcatc gccgggcagg tgatcggagc cctccagacc
//
LOCUS       DAOTHD010000430         1911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_71430_length_1911_cov_3.471983, whole genome shotgun sequence.
ACCESSION   DAOTHD010000430 DAOTHD010000000
VERSION     DAOTHD010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71430_length_1911_cov_3.471983"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="P5219_07660"
     CDS             complement(<1..433)
                     /locus_tag="P5219_07660"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR04274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HRX26672.1"
                     /translation="MNPVRSFPPSARPDAVVLLLGSMPGEASLKAGQYYAHPRNAFWP
                     IMEAVLDIRRDLPYVERLAALAERRIALWDVLASCVRPGSLDSSILGSTAVPNDFPGF
                     FRTHALIRTVFFNGAKAEQAWKRSVAPSLPDLGLQLVRLPST"
     gene            complement(511..>1911)
                     /locus_tag="P5219_07665"
     CDS             complement(511..>1911)
                     /locus_tag="P5219_07665"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRX26673.1"
                     /translation="NDFLLLGDGAGVSKLRQGVTTILVGQCGISAAPIRDERVSLLDA
                     YTGFIRAGVTPEWNWRSFAEYLDVLDGLDLGLNVGSFVGHGTIRLNVLGFENRLPAPD
                     ELREMREMARAALEEGAFGMTSGLIYPPGVYSTSEEIEEIARGLTERRGLYLSHMRNE
                     SSGVVESVRETIAVAERAGIPAQVLHHKATGRANHGKVRETLALLEEARARGVDMTVD
                     QYPYTAGSTTLRSILPPWVNEGGLEKLMERLRDPVLRERIRQEILTTDGWENMLMSCG
                     GPEGVLVAYTPETPEFEGKRLPEIGEMTGRDPLDAAFEVILANRGSDTACYFHMDEDD
                     VKYVLAHPLVMVASDTIPPAPGAKCHPRTNGAFQRVLGRYVREEKVLGLEEAVRKMSG
                     FPAARLGLQKKGLVKPGMDADLVLFNPDTVRDGATFEDPFGEPEGISSVFVAGKLAVK
                     RGVFTGATAGKVLRRS"
BASE COUNT          331 a          690 c          577 g          313 t
ORIGIN      
        1 tggtggaggg caggcggaca agctgcaggc ccaagtcagg cagggacggc gcaaccgagc
       61 gtttccacgc ctgttcggcc ttcgcgccgt tgaagaacac cgtccggata agggcatgcg
      121 tccggaaaaa accgggaaag tcgttgggca ccgccgtgga cccgaggatg gacgaatcca
      181 ggctcccggg ccgcacgcag gacgccagca cgtcccacag ggcgatgcgc ctctccgcca
      241 gggcggcaag acgctcgacg tagggcaggt cccgccggat gtccaggacg gcctccatga
      301 tgggccagaa ggcgttccgc ggatgggcgt aatactgccc ggccttcagg gacgcctctc
      361 ccggcatgct ccccaggaga agaaccaccg catcgggccg ggcggaaggc gggaagctgc
      421 ggacgggatt caccgcagcc tccccggtat cgtgttattg agcattcata cgcctccgga
      481 acatgggaca attcagccgg cgagccggtt tcagcttctc cgcagcactt tccccgccgt
      541 cgctcccgtg aaaacacccc ttttcacagc gagcttcccg gcgacgaaga cagaagaaat
      601 tccttccggc tccccgaagg gatcctcgaa ggtggcgccg tccctcaccg tgtccgggtt
      661 gaagagcacc aggtcggcgt ccatgccggg cttcaccagc cccttcttct gcaggccgag
      721 ccgggccgcg gggaagccgc tcatcttccg caccgcctcc tccaggccca gcaccttctc
      781 ttcccggaca tacctgccca ggaccctctg gaaggccccg ttggtgcggg gatggcactt
      841 cgcccccggc gcggggggga tcgtgtcgga ggccaccatc accaggggat gggccagcac
      901 gtacttcaca tcatcctcgt ccatgtggaa gtagcacgcc gtgtcggagc cgcggttcgc
      961 caggatcacc tcgaaggcgg cgtcaagggg atccctgccc gtcatctccc cgatctcggg
     1021 cagcctcttc ccctcgaatt ccggcgtctc cggggtatag gcaaccaaaa caccctccgg
     1081 accgccacag ctcatcagca tgttctccca gccgtccgtg gtcaggatct cctgccggat
     1141 gcgctcccgg aggacagggt cccggagccg ctccatcagc ttctccaggc cgccctcgtt
     1201 cacccacggc ggcaggatgc tccggagggt ggtgctgccg gcggtgtagg gatactggtc
     1261 cacggtcatg tccacccccc gcgcccgggc ctcttccagc agggcaaggg tctccctcac
     1321 ctttccgtgg tttgcccgcc cggtggcctt gtgatggagg acctgcgccg gaattcccgc
     1381 cctctccgcc acggcgatgg tctcccgcac ggattccacc accccggacg actcgttccg
     1441 catgtgggaa agatagagcc cccgccgctc cgtcagcccc cgagcaatct cctcaatctc
     1501 ctcagaagtg gagtacaccc cgggaggata aatcaggccg ctggtcatgc cgaaggcccc
     1561 ttcctccaga gcggcccggg ccatctccct catctcccgc agctcgtccg gcgcgggaag
     1621 gcggttttcg aatcccagaa cgttcaggcg gatcgttccg tgccccacga aggaccccac
     1681 gttgagcccg agatccagcc catccagcac gtcaaggtat tcggcgaagg acctccagtt
     1741 ccactccggc gtcactcccg ccctgatgaa gcccgtgtag gcgtccagga gggacacccg
     1801 ctcgtcccgg atgggggcgg cgctgatgcc gcactgcccc accaggatgg tggtgacccc
     1861 ctgcctgagc ttggacaccc ccgcaccatc cccgagcaga aggaaatcgt t
//
LOCUS       DAOTHD010000431         1910 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_71458_length_1910_cov_3.615094, whole genome shotgun sequence.
ACCESSION   DAOTHD010000431 DAOTHD010000000
VERSION     DAOTHD010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71458_length_1910_cov_3.615094"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            295..1458
                     /locus_tag="P5219_07670"
     CDS             295..1458
                     /locus_tag="P5219_07670"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRX26674.1"
                     /translation="MPAIRIADRFSSLQPSSMIKIFQAASAVEGLINLSFGEPDFHTE
                     PEIVDAAAKAAREGCTHYPPLQGFEDLRKEIAAYWKRHHGLETDPADILLTVGGLQAP
                     HLCFQAMLNPGDEVLVAEPCFSAYFQQVENNGGVVAPVPSREENGFFPTAEDFAQAVT
                     PRTKILVVNSPCNPTGGVLTMEQAQEIADVAVRHDLFVISDEIYEAFIYSGKHIPLSS
                     LPGMKERTMTVGGFSKSHCMTGWRIGYAMGPAELLKVMTTLSVSQTFGVNTLAQKGGA
                     FALATQDKKVKARTAEFERRVRYCAGRLNAMPGVSCPEPKGAFYLFPDISGTGMADEE
                     FAWWLLEAGKVAVIPGTAFGKSGTGHIRIACTLSMEDLGKAMDRMEEALKKRG"
     gene            1640..>1910
                     /locus_tag="P5219_07675"
     CDS             1640..>1910
                     /locus_tag="P5219_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26675.1"
                     /translation="MRRFTVGALLFFFLAFFPSFSAGGSEGKEKNRPEWVEGEIIVTY
                     RGGEGTGRLETMAARTAGVSVKRFEALSAEQEKNVVFLRVPGKSTE"
BASE COUNT          404 a          544 c          585 g          377 t
ORIGIN      
        1 aatcggatag ggtacagacc gtgattcttc tttgctgaga aagggggctg attttttcga
       61 atcatacggc acttccctat ctttcgcaat atatacattt caccggtccg ctgccatttt
      121 ctccgatctc ctgccggagg aacggaatcg gtcctggctg gttcccctcc cggaggagac
      181 tttgcacccg tcatttcgcg gcgaaaggcc gggtgttgtg gcccgttccg cggtgatgta
      241 tcatatcagc gaaaagaagg ggtacagcct gacattccca aggaggacat tttcatgccg
      301 gcaataagga tagctgaccg tttttcctcg cttcagcctt cgtccatgat caaaatattc
      361 caggccgcct cggccgtgga ggggctgatc aacctgagct tcggcgaacc ggatttccac
      421 accgagccgg agatcgtcga cgccgcggca aaagccgccc gggaagggtg cacccattac
      481 cctcccctcc agggcttcga ggacctgaga aaggaaatag ccgcctactg gaaacgccat
      541 cacgggctgg agacggaccc cgctgacata ctgctcaccg tgggagggct ccaggctccc
      601 cacctctgct tccaggccat gctcaacccc ggcgacgagg ttctcgtggc ggaaccctgc
      661 ttttccgcct atttccagca ggtggagaac aacggcggcg tggtggcgcc cgttccttcc
      721 agggaagaga acgggttctt ccccacggcg gaggatttcg cacaggcggt gacgccgagg
      781 acgaaaatcc tggtggtcaa ctccccctgc aaccccacgg gaggggtgct gaccatggag
      841 caggcccagg aaatcgccga cgtggcggta cggcacgatc tcttcgtcat ctccgacgaa
      901 atttacgagg ccttcattta ctccggcaaa cacattcccc tgtcgtccct tcccggcatg
      961 aaagagcgca ccatgaccgt gggagggttc tccaagagcc actgcatgac cggctggcgg
     1021 atcggctacg ccatgggccc ggcggagctg ctgaaggtga tgaccaccct gtcggtctcc
     1081 cagaccttcg gcgtcaacac cctggcccag aagggcggcg cctttgcact tgccacccag
     1141 gacaaaaagg tgaaggcccg tacggcggaa tttgaaaggc gggtccggta ctgcgctgga
     1201 cggctgaacg ccatgccggg cgtatcgtgt cctgaaccga agggagcctt ttaccttttc
     1261 ccggacattt ccgggacggg catggccgac gaggaattcg cctggtggct tctcgaggcg
     1321 ggcaaggtgg cggttatccc cggcactgcc ttcgggaaga gcggaaccgg gcacatccgc
     1381 atcgcctgca ccctttccat ggaagacctc gggaaagcca tggacagaat ggaggaagcc
     1441 ttgaagaaga gaggctgagg acccggcctg ctttccgaca gttgcagagg aaggggagtc
     1501 gaaaggctct ccttccttat tttctccata gagagtcccg ggagaatacg acagaaggcc
     1561 gttgtccgac aggacccgtc ggggctgaag cggtcccgaa aaaatgttgt tatgacaaac
     1621 gtgaggagat gagagacgga tgagaagatt cacagtggga gcattgctgt tcttttttct
     1681 tgcttttttc ccctcttttt ccgccggagg aagcgaagga aaggaaaaga accggccgga
     1741 atgggtggag ggtgagatca tcgtcaccta ccgcgggggg gaaggcacgg gtcgtctgga
     1801 gaccatggcg gcccggactg cgggagtctc ggtgaaacgg ttcgaggccc tgtccgctga
     1861 acaggaaaaa aatgtcgtgt tccttcgggt tccgggaaaa tcgacggagg
//
LOCUS       DAOTHD010000432         1907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_71623_length_1907_cov_2.811555, whole genome shotgun sequence.
ACCESSION   DAOTHD010000432 DAOTHD010000000
VERSION     DAOTHD010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71623_length_1907_cov_2.811555"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1695
                     /locus_tag="P5219_07680"
     CDS             <1..1695
                     /locus_tag="P5219_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HRX26676.1"
                     /translation="GFPKEEVTGIMSLSAFFRWLERHFRNTALMILFLAALNLGVYVL
                     VLATGGIKYVYSHSMYIPILLAGFIFGARGGVLAGLAGGVVLGPFMPIETATGEPQLL
                     LNWLYRTGLFTLIGLFNGVAVEMLRRQLRENEWLARHDPFTRLPNRVALMEYIEASGR
                     EGGEAGTASSALFAVFAENLMEAGATFGVRITDEIMVQMHDRLQGLFPHGTPVFSTQP
                     GELSVILPCGGAEEARDEMDRISAAMKEPFVLPDMSLHVEAVAGCVMLEKNGEDGHAV
                     LRKADIAAHSAGRKGQESLLYDSELDGTIAENLEMLGALKGAMEKGELLLHYQPKVSM
                     RDGRVVGAESLLRWGHPVRGLVYPASFVPQAEKSNLMNALTEWVINEALDQSVRWRKE
                     GIVLSLAVNVSARDLTSPRFAEMILEALARRDLDGDSLELEITESAIMEDPLHATGVL
                     KRFSDVRVVISIDDFGTGYSSLQYLSRFPATVIKVDQSFVRNLRSDDGARHIIDAAVR
                     LAHALGMEVVVEGVESEETFEMLREMGCDIAQGYGVSPPLSVDEFSGWFFSRTMNG"
BASE COUNT          353 a          554 c          613 g          387 t
ORIGIN      
        1 ggcttcccga aggaggaggt gaccggcatc atgtctctgt ccgcattttt ccgctggctt
       61 gaaaggcact tcaggaacac agcactcatg atactgtttc tcgccgccct gaacctcgga
      121 gtttacgtcc ttgtccttgc aacggggggt atcaagtacg tttactccca ttccatgtac
      181 atccccattc tcctggcggg gttcattttc ggcgcccggg ggggcgttct tgccggcctg
      241 gccggcgggg ttgtcctcgg tcccttcatg cctatcgaaa cagccaccgg cgagccccag
      301 cttctgctga actggctcta ccggacaggg ctgtttaccc tgatcggcct gttcaacgga
      361 gttgcggtgg aaatgctccg gcgccagctc agggagaacg aatggctggc ccggcatgac
      421 ccctttaccc ggcttcccaa cagggtcgct ctcatggaat acatcgaggc atccggcagg
      481 gaaggcgggg aagctggaac ggcttcttcc gcccttttcg ccgttttcgc cgaaaacctc
      541 atggaggcgg gggccacttt cggagtccgg attacggacg aaatcatggt gcagatgcat
      601 gaccgcctgc agggcctctt cccgcacggg actcccgttt tcagcaccca gcccggggag
      661 ctgtcggtga ttctgccctg cggaggggcg gaggaggccc gggatgagat ggaccgcatc
      721 tctgcggcga tgaaggaacc tttcgttctg ccggacatgt ccctgcacgt ggaagcagtc
      781 gcgggctgcg tgatgctcga gaaaaacggg gaggacggcc acgccgtgct ccggaaggcg
      841 gatatcgccg cccatagcgc gggacgaaag gggcaggaaa gcctgctcta tgacagtgag
      901 cttgacggaa ccatcgcgga aaaccttgag atgctgggtg cgctgaaggg cgccatggag
      961 aagggagagc tgcttcttca ctaccagccg aaagtgtcca tgagggacgg ccgggtggtg
     1021 ggggcggaat ccctgctgcg gtgggggcac cccgtccggg ggctggtgta tcctgcctcc
     1081 ttcgttcccc aggcggagaa gagcaacctg atgaacgccc tcacggagtg ggtgatcaat
     1141 gaagccctgg accagtccgt ccggtggagg aaggagggaa tcgtcctctc tctcgccgtg
     1201 aacgtgtcgg ccagggatct cacgtcaccc cggttcgctg agatgatcct cgaagccctc
     1261 gcccgaaggg acctggacgg agatagcctc gagctggaaa taaccgaaag cgccatcatg
     1321 gaggatcctc tgcacgccac cggggtactg aagcgctttt ccgatgtgcg ggtggtcatc
     1381 tccatcgacg atttcggcac gggatactcc tcactgcagt atctttcccg tttccctgcc
     1441 acggtgatca aggtggacca gtcttttgtc cggaatctcc gctccgacga cggtgcccgg
     1501 catatcatcg acgctgcggt gcgcctcgct cacgccctgg gaatggaggt tgtggtggag
     1561 ggggtcgagt cggaggagac ctttgagatg ctccgggaga tgggctgcga catcgcccag
     1621 ggatacggcg tttcaccgcc cctttccgtg gatgaattca gcggctggtt tttcagcaga
     1681 acaatgaacg gctgatcatc cgcgcgtttc cgtgggggcc gctgcccaaa aagagaaggg
     1741 aacgcaaaaa acggcacccc cggttccagc cagggagtgc cgttttttgt gttcctgaat
     1801 ctttcgtccg gcctcaggaa ttttcctggc tgattatgcg gtagacgatg gccccgagga
     1861 tcgctccggc gatgggggcg gcccagaaga gccacagctc ggaaatg
//
LOCUS       DAOTHD010000433         1905 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_71704_length_1905_cov_3.362162, whole genome shotgun sequence.
ACCESSION   DAOTHD010000433 DAOTHD010000000
VERSION     DAOTHD010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71704_length_1905_cov_3.362162"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="P5219_07685"
     CDS             <1..730
                     /locus_tag="P5219_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRX26677.1"
                     /translation="YERPKSWMPNVSSHYCPGCGHGIAHRLVCEVINELGIQNDTIGV
                     APVGCAAMIYDYMDTDYCEAPHGRAPAVATGLKTVRPDKIVYTYQGDGDLASIGMGEI
                     VHAANRGQNITVIFINNAIYGMTGGQMAPTTLLGQKATTCPLGRDAKVNGYPIRVSEM
                     LATLPAPGYITRVTVAQPKYIIKAKQAIKKAFQNQVEGKGFSLVEVLSSCPTNWGMRP
                     VEAFDWLISSMMPYYPLGDFKDFD"
     gene            750..1310
                     /locus_tag="P5219_07690"
     CDS             750..1310
                     /locus_tag="P5219_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRX26678.1"
                     /translation="MSAFYKTLLAAGFGGQGVMVLGQLVAYTGIEEGRHVTWIPSYGP
                     EMRGGTANCGVVLSEEEIGSPVVDSADVLVIMNQPSLSKFLDRVKPGGVLVYNSDLVE
                     YDGSRKDIAAVGLPASTMAYELGSDKITNIVILGAVVESSGIVNNDVCVETIKEKLGK
                     KKPKFLPMNLEAFEKGKAAARAALSR"
     gene            1324..1863
                     /locus_tag="P5219_07695"
     CDS             1324..1863
                     /locus_tag="P5219_07695"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRX26679.1"
                     /translation="MDTREKTISKTLAYRGSILNLRIDKVEIPGGRKTIREVVEHSPA
                     VAILPVEADGTVYLIRQYRYAIGDEILEIPAGLVEPGEDFERCAERELQEEIGYFPGT
                     LREIGRMYNSPGFCTEMLILYFATDLRPSKLQADDDEFIRLQPVPPGEIRKLLEEGKI
                     IDGKTFAALTWYLAFQEEN"
BASE COUNT          418 a          580 c          565 g          342 t
ORIGIN      
        1 gtacgaacgg ccgaagagct ggatgccgaa cgtgagcagc cactactgcc cgggttgcgg
       61 tcacggcatc gcccaccggc ttgtctgcga ggtcatcaac gaactcggaa ttcagaacga
      121 tactatcggt gtggcccccg tgggctgtgc cgccatgatc tacgactaca tggacaccga
      181 ctactgcgaa gccccccacg gcagggctcc cgcagtggcc acgggactga agaccgtccg
      241 ccccgacaaa atagtctata cctaccaggg cgacggcgac ctggcctcca tcggcatggg
      301 cgagatcgtc cacgccgcga accgcggcca gaacatcacc gtgatcttca tcaacaacgc
      361 catctacggc atgaccggag gccagatggc ccccaccacc cttctcggcc agaaggccac
      421 cacctgcccc ctgggacggg acgccaaggt gaacggctat cccatccggg tgagcgagat
      481 gcttgccacc ctacccgccc ccggatacat caccagggtg acagtggccc agccgaagta
      541 catcatcaag gcgaagcagg ccatcaagaa ggccttccag aaccaggtcg aaggcaaggg
      601 attctccctt gtggaagtgc tttcatcctg cccgaccaac tggggcatgc gccccgttga
      661 ggcctttgac tggctcatca gctccatgat gccctactat ccccttggcg acttcaagga
      721 tttcgactag gaaaggggga gaaaaaacaa tgtctgcctt ctacaaaacg cttctcgcgg
      781 cagggttcgg cggccaggga gtcatggtcc tcggtcagct tgtcgcctat accggtatcg
      841 aggaggggcg gcacgtgacg tggatccctt cctacggtcc ggagatgcgg ggcggcacgg
      901 caaactgcgg cgtggtcctc tccgaggagg aaatcggttc tcccgtggtg gacagcgccg
      961 atgtcctggt gatcatgaac cagccctccc tctccaagtt ccttgacagg gtgaagcccg
     1021 gcggcgtcct ggtgtacaac agcgacctgg tggagtacga cggctcccgg aaggacatcg
     1081 ccgcagtggg attgcccgct tcgaccatgg cctacgaact cggaagcgac aagatcacca
     1141 acatcgtgat cctcggggct gtggtcgagt ccagcggaat cgtgaacaac gacgtctgcg
     1201 ttgagaccat caaggaaaag ctcggaaaga aaaagcccaa gttccttccc atgaaccttg
     1261 aagccttcga gaagggaaaa gcggctgcac gggctgccct ttccaggtag ctccggcttc
     1321 ttcatggata ccagggaaaa gactatctcg aaaaccctgg cctaccgggg cagcatactg
     1381 aatctccgga tagacaaggt agagatcccc ggaggacgga agaccatccg ggaggtggtg
     1441 gaacattccc cggcggtggc catccttccc gtggaggccg acggaacggt ctatctcata
     1501 cggcagtacc ggtatgccat aggcgacgag atcctcgaga tccctgcggg acttgtggag
     1561 ccgggagagg attttgaacg gtgcgccgag cgtgagctcc aggaggagat cggttacttt
     1621 cccggaacgc tgcgggagat cggccggatg tacaactctc cgggcttctg cacggaaatg
     1681 ctcatcctgt acttcgccac ggacctgcgg ccgtcgaaac tccaggcgga cgacgacgaa
     1741 ttcatccgtc tccagcccgt gccccccgga gagatccgga agctgctgga ggaagggaag
     1801 atcattgacg ggaagacctt cgcagccctg acctggtatc tggcttttca ggaagaaaat
     1861 tgatcctttc cgggaccgga aatctccggt cccggttttt ttatg
//
LOCUS       DAOTHD010000434         1904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_71748_length_1904_cov_3.499189, whole genome shotgun sequence.
ACCESSION   DAOTHD010000434 DAOTHD010000000
VERSION     DAOTHD010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71748_length_1904_cov_3.499189"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="P5219_07700"
     CDS             <1..239
                     /locus_tag="P5219_07700"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26680.1"
                     /translation="LLPDLLVPGAALFGKTRSRGEPLPLTVLECEGDGLLVRFAEPHF
                     APAPGQRLVVYDKEGRVAGGGVIRHRKEEEEWTA"
     gene            227..1063
                     /locus_tag="P5219_07705"
     CDS             227..1063
                     /locus_tag="P5219_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HRX26681.1"
                     /translation="MDSVISRRLADLYETGQAAVLCIVVEESGSTPRSTGSSMIVFPG
                     GEIEGTVGGGVTEHRVIEKALELLKKGTGSLVYRETLSATEAALEGAACGGSMGVYLE
                     VIGRRRELVIFGGGHVGKAIARLGDMTGFAVTVWDEREEFANPAHIPWGKTLCCSLEE
                     LPGKMPRFHGDTYAIIVTRGHVLDADVVKLLEGREAAYMGMIGSQRKIAFVREHLLGQ
                     GVSPEFLDGIYQPVGLPIGAETPEEIAVSVLAEIVAVARGGDVQALRNAGAMKNGKNH
                     SK"
     gene            1182..>1904
                     /locus_tag="P5219_07710"
     CDS             1182..>1904
                     /locus_tag="P5219_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRX26682.1"
                     /translation="MAVSYKDYYEILGVSRTASQEEIRKAFRKLAKEYHPDVNREAGS
                     GEKYREINEAYEVLKDPEKRKKYDTLGADWSQGQDFTPPPGWGGGVHMDFGGDAGGFS
                     DFFRTIFGGFSGGARSASMEDMFFGGGTARGRDQEMVLELSLEELLKGDTKTVSFERT
                     EPGPDGRLRKGRKTVNVNLPGGVTDGSRIRLKGQGSGGGDLFLVLRIAPHPRFAVEGH
                     DIATVLKVSPWEAALGASLPVDT"
BASE COUNT          429 a          495 c          629 g          351 t
ORIGIN      
        1 tccttcttcc ggacctgctc gttcccggcg cggccctttt cggaaagacc aggtcacgag
       61 gggagcctct gcccctcacc gttctggaat gcgaaggaga cgggcttctt gtccggtttg
      121 cggagcctca tttcgcaccg gcgccgggac agagactcgt ggtgtacgat aaggaaggac
      181 gggtggccgg aggaggcgtc atccgccacc ggaaggagga ggaagaatgg acagcgtgat
      241 ttcccggcgt ctcgccgatc tttacgaaac aggacaagct gcggttcttt gcattgtggt
      301 ggaggaatcg ggctccacac cgagaagcac cggttcatcc atgatcgtct tccccggagg
      361 ggaaattgaa ggaaccgtgg gcggaggtgt cacggaacac agggtgatag aaaaggccct
      421 ggagctcctg aaaaaaggga ccggttccct ggtgtacagg gaaaccctgt ccgccacgga
      481 ggcagctctc gaaggcgcgg cctgcggcgg ttccatgggg gtctacctcg aagtgatcgg
      541 cagacggaga gagctcgtta tcttcggcgg cggccacgtc ggaaaggcca tcgcgaggct
      601 cggcgacatg acgggatttg ccgttaccgt atgggatgag cgtgaggagt tcgcaaatcc
      661 tgcacatatt ccctggggga aaaccctgtg ctgttccctg gaggaacttc cgggaaagat
      721 gccccgcttt cacggggaca cctatgcaat catcgtgacc aggggccatg tcctggacgc
      781 cgacgtggtg aaactccttg agggcaggga agcggcctac atgggcatga tcgggtcaca
      841 gaggaagatc gccttcgttc gggagcacct tctcggccag ggggtatccc cggaattcct
      901 ggacgggatt taccagcccg tgggtcttcc catcggtgct gagacccccg aggagatagc
      961 agtctccgtc ctggcggaaa tcgttgctgt cgcaagggga ggcgatgtgc aggccctccg
     1021 gaacgcagga gcaatgaaaa acggaaaaaa tcacagcaaa tgaaagaaaa agctgaaaaa
     1081 cgggccttta ttcctctccg gtgcgtttga gatgatggaa gaaaaaggta caatataaag
     1141 gtcagcattg gttagatttt ttttggaaag caggtgggga catggccgtc tcgtacaagg
     1201 actactatga aatactcggc gtcagcagga cggcttccca ggaggaaata cggaaggcct
     1261 tcaggaagct ggcgaaggaa taccatcccg atgtcaacag ggaagccggc tccggggaaa
     1321 aatacaggga aatcaatgaa gcctacgagg tcctgaagga tcctgaaaag cggaagaaat
     1381 acgataccct cggtgcggac tggagccagg ggcaggattt cactcccccc ccgggatggg
     1441 gcggcggggt tcacatggat ttcggcggag acgcaggcgg cttcagtgat tttttccgga
     1501 ccatcttcgg cggcttttcc gggggagccc gctccgcttc catggaggac atgttcttcg
     1561 gcggcggaac cgcccggggc cgggaccagg aaatggtcct cgagctttct ctcgaggagc
     1621 tgctgaaggg agacacaaag acggtctcct ttgaaagaac cgaaccaggg ccggacggga
     1681 gactgcgaaa agggagaaaa acggtgaacg tgaatctccc cgggggagtg acggacggat
     1741 ccagaatccg cctgaagggt cagggaagcg gcggcgggga tctctttctc gttctgagga
     1801 tagcccctca tccccggttc gccgtggagg gacacgacat cgccactgtc ctgaaggtgt
     1861 ctccctggga ggcggccctg ggtgcgagcc ttcccgtgga tacc
//
LOCUS       DAOTHD010000435         1904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_71772_length_1904_cov_2.194159, whole genome shotgun sequence.
ACCESSION   DAOTHD010000435 DAOTHD010000000
VERSION     DAOTHD010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71772_length_1904_cov_2.194159"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..>1904)
                     /locus_tag="P5219_07715"
     CDS             complement(77..>1904)
                     /locus_tag="P5219_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26683.1"
                     /translation="MPGFADGGEKTGGPEGGKKSPQEEFFASLGLDKVVLGEDDLREI
                     EEYRETMKNAGLGAEQIEEAVRGFELAKKMSALGPAAMMKAGRSAAKKQDSKTETRET
                     GAVGDAPLPPAAEGYNAGDEQEKARWALGLADHMAVASFYDISDAGMVRAAGLAVSAY
                     PHPLLLNNYAGMIGEISPDDALFFYFSALSLEPRNPVILTNIAMSFLDRNDSGAARNY
                     ATRALSENPEFGPAFQVLTICHLRDGNSVLAAETLMKSARDCFDDLSVRLFDDYLEEV
                     GNLDVSEGDEYPLSEQQLEILYQIAKKYGDPERENGADTPGAQIRINPFPVFGSADHY
                     LNSMDFLVGDAERRTAKLSKISAELSRLEKAVFGKQGDRGALPVMKNMRQYYAYLVLE
                     SYYHFKMRELNWKLQEEDPTDDLLKDYVEAYMTQCRSFRKQEHDFHEAFLKQTKGGPK
                     EEAEWYRKGIRFYSACLDHQKMLLDITRRYTVEYLKIPKSKYDERKPLVEEFWLKAGG
                     LMKYMTDGNVYNLADKRREMFAVDWMDIWPTAGYPVPNAGDSVFVRAVDEAPLGMSSP
                     YLCSISPPALQGALENMQRYQEELAKLSKGLNRLNPPPKVLP"
BASE COUNT          331 a          595 c          556 g          422 t
ORIGIN      
        1 ggggcagatc ccccggttcc ttgaacagag gaagggccgt tcgttcgatc tccacggggt
       61 cagcgtcaaa ggcatattac ggcaggacct tcgggggcgg gttcagccgg ttgagccctt
      121 tgctcagttt cgccagctct tcctggtacc gctgcatgtt ctcaagggct ccctggaggg
      181 caggagggga gatagagcac aggtaaggcg acgacatgcc caggggagct tcgtccacgg
      241 ccctgacgaa cacggaatct cccgcgttgg ggacagggta ccccgctgtg ggccagatat
      301 ccatccagtc cacggcaaac atctcccgcc tcttgtcagc aaggttgtac acattcccgt
      361 ccgtcatgta cttcatcaag ccgcccgcct tcagccagaa ctcctccacc aggggctttc
      421 gttcgtcgta tttgctcttc gggatcttca ggtattcgac ggtataccgc cgggtgatgt
      481 ccagcagcat cttctggtgg tcgagacatg ccgagtagaa tcggattccc ttccggtacc
      541 attccgcctc ctccttcggg cctcccttgg tctgcttcag aaaggcttcg tgaaaatcgt
      601 gctcctgctt ccggaaggac ctgcactggg tcatgtaggc ttccacatag tctttcagaa
      661 ggtcgtccgt ggggtcctcc tcctggagct tccagttgag ctccctcatt ttgaagtggt
      721 agtacgattc cagcaccagg taggcatagt actggcgcat gttcttcatc accggcaggg
      781 cgccccggtc cccctgcttc ccgaagacgg ctttttccag gcgggacagc tctgcggaaa
      841 tcttcgacag ttttgccgtt cgccgttcgg cgtccccgac gaggaaatcc atggaattca
      901 ggtaatggtc cgcgctcccg aagacaggga aagggttgat ccggatctgg gctcccgggg
      961 tgtcggcacc gttctcccgc tcagggtcgc cgtacttctt cgcgatctgg tacaggatct
     1021 ccagctgctg ttcggaaaga ggatactcgt ccccttcgct cacatcgaga ttgcccactt
     1081 cctcgaggta atcgtcgaac agccgcacgg acaggtcgtc gaaacagtcc ctggccgatt
     1141 tcatgagcgt ttcggcggcg aggacgctgt ttccgtcccg aagatggcag atggtgagca
     1201 cctggaaggc gggaccgaac tcggggttct ccgacagggc ccgggtcgcg tagttccggg
     1261 ccgccccgga atcattcctg tcaaggaagg acatggcgat gttggtcagg atcaccgggt
     1321 tccggggttc cagggagagg gcggaaaagt agaagaagag ggcgtcatca ggtgaaatct
     1381 ccccgatcat cccggcgtag ttgttcagga ggaggggatg gggataggcg gagacggcga
     1441 gccccgccgc cctcaccatg cccgcgtcgg aaatgtcgta gaaggaggcc acggccatat
     1501 ggtccgccag gccgagggcc caccgggctt tttcctgttc gtccccggcg ttgtatcctt
     1561 ccgccgccgg cggcagcgga gcgtctccca cggcaccagt ttcgcgggtt tccgtcttcg
     1621 aatcctgctt cttcgcggcg cttctgccgg ccttcatcat ggccgcaggc cccagagcgc
     1681 tcatcttctt cgccagttca aaccccctca cggcctcctc gatctgctcc gcaccgagcc
     1741 cggcattctt catggtttcc ctgtattctt cgatctcccg gaggtcgtcc tctccaagga
     1801 cgaccttatc gagtcccagg gaggcgaaga actcctcctg aggagatttt tttccgcctt
     1861 caggtccgcc cgtcttttca cccccgtcag cgaaaccggg catc
//
LOCUS       DAOTHD010000436         1903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_71811_length_1903_cov_2.970779, whole genome shotgun sequence.
ACCESSION   DAOTHD010000436 DAOTHD010000000
VERSION     DAOTHD010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71811_length_1903_cov_2.970779"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="P5219_07720"
     CDS             <1..646
                     /locus_tag="P5219_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRX26684.1"
                     /translation="TVTSIRAISPEEALDRLRAKLGNQARAVTLMGENPLPWSLEIQV
                     QRAEHITPLVRDLVVFPSVEEVIYSGKLVERLSTISRASAGVSATILVLAVIISALVI
                     FNTIRIAIYSRKEEIEVMLLIGATKTYISLPFVFQGMFLGAGGALLAVAGLWATYASA
                     VNAVRSTLAFLDIIVNQQILLQFFLLLFATGATMGWMCSWLAVSRFTSRALRPR"
     gene            671..1858
                     /locus_tag="P5219_07725"
     CDS             671..1858
                     /locus_tag="P5219_07725"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRX26685.1"
                     /translation="MKTARTACFLLAIALFLASAGPLSGASGLDSRIAQEEARMRVLE
                     KQISEHQKRARQMGEKEKGVLTQLNSLDQKKKVTEQRIRVLELKLEKVKNTIAGLRAE
                     VRLTEQELGEMMYVFENRLVDIYKYGGVAEFNLLLSSTTAHEAMETSYLLNRIALQDQ
                     AMISEMLQKKDRLIKAAAQMEQQEKELSENTSRLNGERKTFRNEISKSNVFLGKVRQE
                     RSLHEQAVKELLQSQKEIQQTVTALMRKKRDEESRNKGRSAQYTYLPSGGQLSWPVQG
                     QITSTFGQRVHPVFKTKMMHTGMDIRAPRGTPVRAAGPGEVLFAGWLRGYGQVIIIDH
                     GNNLSSVYAHLSSMSVREGSAVKKGQTIGAVGSTGTATGAHLHFEVRVGGDARDPMRY
                     LRK"
BASE COUNT          431 a          570 c          566 g          336 t
ORIGIN      
        1 cacggtcacc agcatccggg ccatttcgcc ggaggaggct ctcgaccgcc tccgggcgaa
       61 gctgggaaac caggccaggg ccgttaccct catgggggaa aaccccctgc cgtggagcct
      121 ggaaatccag gtccagcggg cggagcacat tacgcccctc gtccgggacc tcgtggtctt
      181 cccctccgtg gaggaagtga tctactcggg caagctggtg gagcgccttt ccaccatatc
      241 ccgggcatcg gcgggcgtgt cggccaccat cctcgttctc gcggtgatca tcagcgccct
      301 ggtgattttc aacaccatcc ggatcgccat ctactccagg aaggaagaga tcgaggtcat
      361 gctcctcatc ggggccacca agacctacat ctcccttcct ttcgtcttcc agggaatgtt
      421 cctcggcgcc gggggagccc tgctcgccgt ggcgggcctc tgggccacct acgcgtcggc
      481 ggtgaacgcc gtacgctcca ccctcgcgtt tcttgatata attgtcaacc agcagatcct
      541 gctccagttc ttccttcttc ttttcgccac gggcgccacc atgggctgga tgtgcagctg
      601 gctcgccgtg agccgtttta cgtcgagggc cctgcgcccc cgctgactcc gacgaaggga
      661 gctgaccaca gtgaagaccg cacgaacagc ctgttttctc cttgcgatcg ccctgtttct
      721 tgcttccgcg ggtcccctgt ccggcgcttc gggcctcgac agcaggatag cccaggaaga
      781 ggcccggatg agggttctgg aaaagcagat ttccgaacac cagaagcggg ccaggcagat
      841 gggggagaaa gaaaagggag tgctcaccca gctcaattcc ctggaccaga agaagaaggt
      901 gaccgaacaa agaatacggg tgctggagct gaagcttgaa aaagtaaaaa acaccatcgc
      961 ggggctcagg gcggaagtcc gcctgaccga gcaggagctt ggggagatga tgtacgtctt
     1021 tgaaaaccgt ctcgtggaca tctacaagta cggcggcgtg gcggagttca acctccttct
     1081 ttcctccacc accgcccacg aggccatgga gacctcctac ctgctgaacc gcatcgccct
     1141 ccaggaccag gccatgatca gcgaaatgct ccagaaaaag gatcgtctca tcaaggccgc
     1201 cgcccagatg gagcagcagg aaaaagagct ttctgagaac acgtcccgcc tcaacggcga
     1261 acgaaagaca ttccggaacg agatcagcaa gagcaatgtc tttctgggga aggtacgaca
     1321 ggagcggtca cttcacgaac aggccgtgaa ggagcttctc cagtcccaga aggaaatcca
     1381 gcagaccgtg acggccctca tgcggaaaaa gcgggatgag gagtcaagga acaaaggacg
     1441 gtccgcccag tatacctatc ttccttcggg ggggcagctt tcctggcccg tccagggaca
     1501 gatcacgagc accttcggcc agagggtgca tcctgtcttc aagacgaaaa tgatgcacac
     1561 cggcatggac attcgggcac ccaggggaac gcccgtacgg gccgccgggc ccggcgaggt
     1621 tctctttgcc ggatggctga gagggtacgg acaggtgatc atcatcgacc acgggaacaa
     1681 tctgtcctcc gtctacgccc acctttcgag catgtcggta cgggaaggct cggcggtcaa
     1741 gaaaggacag acaatagggg cagtgggcag cacgggaacg gccacagggg cacaccttca
     1801 tttcgaggtg cgtgtcggcg gcgatgccag ggatcccatg cggtatttga gaaaatagaa
     1861 aaggagaagg acaatgtccg aacagaaaac acactttcga aaa
//
LOCUS       DAOTHD010000437         1902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_71863_length_1902_cov_3.425014, whole genome shotgun sequence.
ACCESSION   DAOTHD010000437 DAOTHD010000000
VERSION     DAOTHD010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71863_length_1902_cov_3.425014"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1902
                     /gene="ppdK"
                     /locus_tag="P5219_07730"
     CDS             <1..>1902
                     /gene="ppdK"
                     /locus_tag="P5219_07730"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRX26686.1"
                     /translation="CLEYGKDEKFLDSIWDDVTAAMRALEKETGKTFGSGQNPLLVSV
                     RSGAPISMPGMMDTVLNLGLNDESVESLAKAASDRRFAFDSYRRFIQMYGNVVHGVNG
                     DAFEEVLEHVKKGLGVTFDNEIPEKSLVEIVAKFKEIFKKAAGFDFPSDPWKQLRNAV
                     EAVFKSWGNPRAITYRKLHKIPHTLGTAVNIVSMVYGNLGDDCGTGVCFTRNPSDGTK
                     ELYGEFLVNAQGEDVVAGIRTPLPIAQLKDAMPAVYDELFEITTNLEKAYKDMQDIEF
                     TIERNKLYILQTRNGKRSAGAAVKIAVDMVNEGLIDKKTAVGRVTPEQVEMLLHRQVD
                     PKAENEVLAKGLPASPGAGVGVVCFDADDAVELAKTGKKVILARPETCPDDIHGLFAA
                     EAIVTSRGGMTSHAAVVARGLGKPCVSGCEDIFIDLNKETISKGDKVVRKGDTITVDG
                     STGRVLLGGAPLMEASFSDDFKTVLSWADEYARLQVWANGDTPEDARRARAFGAEGIG
                     LCRTEHMFMAADRLPVMQRLVVADSYEERVAALDELKVMQKEDFYGILKEMQGLPVII
                     RLLDPPLHEFMPKERDLEEELAELVAAGKEHSPEADRVRKVMAKVEALKESNPMLGFR
                     GCRLGVVYP"
BASE COUNT          399 a          626 c          568 g          309 t
ORIGIN      
        1 cctgccttga atacggcaag gacgagaaat tcctcgactc catctgggac gacgtgactg
       61 ccgccatgcg ggccctcgag aaggaaacgg gaaagacgtt cggctcgggg cagaatcccc
      121 tcctcgtgtc cgtccggtcc ggtgctccca tttccatgcc cggcatgatg gacacggtgc
      181 tcaacctcgg gctcaacgac gagtcggttg agtccctcgc caaggccgcc agcgacaggc
      241 gtttcgcttt cgacagctac cgccgcttca tccagatgta cggcaacgtg gtccacggtg
      301 tcaacggaga cgccttcgaa gaagtgcttg aacacgtcaa gaagggactc ggcgtgacct
      361 tcgacaatga gattcccgaa aagagtcttg tggagatcgt ggcgaagttc aaggagatct
      421 ttaaaaaggc ggcagggttc gacttcccgt cggatccctg gaagcagctc cgcaacgccg
      481 tggaggcggt attcaagagc tggggaaatc cccgggccat cacctaccgg aagctccaca
      541 agatccccca caccctcggc acggcggtca acatcgtctc catggtctac ggaaatctcg
      601 gcgacgactg cggaaccggg gtgtgcttca cccgcaaccc ctctgatgga accaaggaac
      661 tctacggcga gtttcttgtg aacgcccagg gcgaggacgt ggtggccggc atcaggaccc
      721 ctcttcccat cgcccagctc aaggacgcca tgcccgctgt ctacgacgag cttttcgaga
      781 ttaccaccaa ccttgagaag gcctacaagg acatgcagga catcgagttc accatcgagc
      841 gcaacaagct gtacatcctc cagacccgca acggcaagcg ttccgccggt gctgcggtga
      901 agatcgccgt ggacatggtg aacgaggggc tcatcgacaa gaagaccgcc gtcggcaggg
      961 tcaccccgga gcaggtggaa atgctcctgc accggcaggt cgacccgaag gcagagaatg
     1021 aagtgctcgc caaggggctt cccgcatctc ccggcgccgg cgtgggcgtt gtctgcttcg
     1081 atgccgacga cgccgtcgag ctggccaaga ccggcaagaa agtcatcctt gcccggcccg
     1141 agacctgccc cgacgacatc cacggcctgt tcgccgccga agccatcgtc accagccgcg
     1201 gaggcatgac aagccacgcc gccgtggtcg cacgggggct cggcaagccc tgcgtcagcg
     1261 gatgcgagga catcttcatc gacctgaaca aagagaccat ctcaaagggc gacaaggtcg
     1321 tccgcaaggg agacaccatc accgtggacg gcagcacggg ccgcgttctt ctcggcggtg
     1381 ccccgctcat ggaagcctcc ttcagcgacg acttcaagac cgtcctttcc tgggccgacg
     1441 aatatgcccg tctccaggtc tgggccaacg gcgacacccc cgaggacgcc aggcgtgccc
     1501 gggccttcgg cgccgagggc atcggcctct gccgcacgga gcacatgttc atggccgccg
     1561 accgccttcc cgtcatgcag cgcctcgtgg tggccgacag ctatgaagag cgcgtcgccg
     1621 ccctcgatga gctcaaggtc atgcagaagg aagacttcta cggcatcctg aaggaaatgc
     1681 agggccttcc cgtcatcatc cgcctcctcg acccgcccct ccacgagttc atgcccaagg
     1741 agagggacct cgaggaagag ctcgccgaac tcgtcgcagc ggggaaagaa cactcgcccg
     1801 aggccgacag ggtccgcaag gtcatggcca aggtggaagc cctcaaggaa tccaacccca
     1861 tgctcggctt ccggggatgc cgtctcggag tggtctatcc tg
//
LOCUS       DAOTHD010000438         1900 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_71962_length_1900_cov_2.896477, whole genome shotgun sequence.
ACCESSION   DAOTHD010000438 DAOTHD010000000
VERSION     DAOTHD010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71962_length_1900_cov_2.896477"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..>1900
                     /locus_tag="P5219_07735"
     CDS             211..>1900
                     /locus_tag="P5219_07735"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRX26687.1"
                     /translation="MTLQHVGKSVPRVDAVKKATGEACFVADITLPRMLRAKVVRAGI
                     PHGKILSIDTSAAEAMPGVKKVVTGQGCSMLFGTCLWDQPPLAVDKVRHAGEAVAVVL
                     AETENQAEAAAKAVKAEYEKLPFVLDPVEAAAPGAPLIHEKNGSYRRVEYSVHPVPGT
                     NIFHHYKLRKGESEKGFADADVTVEGEFEFPISSQAPIEPHGAICRFSADGSMEIWAS
                     NQAPFVLRDVLADMFTIPASKVRVHIPYLGGGFGGKSDVSIEPLVAYAASFVPGYAVK
                     LVLTRKEVFTSALVGRGMKGRMKIGAKKDGTFTALKAEMYFADGAYGDTGWPVDTVAG
                     HNCTGPYEFPHVTVDVYGTYTNSPIVGAYRGYGHPEGQLMSGRLIDRLARKLGMTPGE
                     IMRKNFLSEGRQNALGQVIKKSHGDLPGCLAAVEKALDETPLPKNDDRFLYGRGIASM
                     MKSPKMAANAASTCTVQVNADGSAFVNLSGIEMGQGVQTVFAQMAAEALQIPMEKVKS
                     YPDVDTQFSPWEWQTVASMQTYRGGRAIQDACGQIIDIARETAAKVWNCSPSLVA"
BASE COUNT          372 a          630 c          607 g          291 t
ORIGIN      
        1 ccagcgccgg ctccaggggc tgggcttcga catccccttc cgcctgttcc ttctcatccc
       61 ctacctgatg accgttgtcg cccttgtcag cgtgtcgcgg agggccgctg ccccggcggg
      121 gctcctgaaa ccctaccggc gggaggaaaa aggctaaagg ctttcccctc cggggagaaa
      181 gcgcactatc gaatgacggg agagtgaatc atgacgcttc agcacgttgg aaaaagcgtc
      241 cccagagtgg acgcggtaaa aaaagcgaca ggcgaggcct gtttcgttgc cgacattacc
      301 cttccccgga tgctccgcgc aaaggtcgtc cgggcaggca tcccccacgg aaagatcctg
      361 tccatcgaca cctcggcggc ggaagccatg cccggggtga agaaggtggt cacggggcag
      421 gggtgctcca tgctcttcgg cacctgcctg tgggaccagc cgcccctggc ggtggacaag
      481 gtccgccacg ccggcgaagc cgtggccgtg gtgctggcgg agacggagaa ccaggccgaa
      541 gctgcggcga aggcggtgaa agcggagtac gagaagctcc ccttcgtcct cgatcccgtg
      601 gaggccgccg cccccggggc gcccctgatc catgagaaaa acggctcgta ccgcagggtg
      661 gagtactcgg tccatccggt gccgggaacc aacatcttcc accactacaa gctccgcaag
      721 ggcgagagcg aaaaaggctt cgccgacgcg gacgtcaccg tggagggcga gttcgagttc
      781 cccatttcca gccaggcgcc catcgaaccc cacggggcga tatgccgctt cagcgccgac
      841 gggagcatgg agatctgggc ctccaaccag gcccccttcg tcctcaggga cgtgctggcc
      901 gacatgttca caatccccgc gtcgaaggtc cgggtccaca ttccctacct cggcggcggc
      961 ttcggcggca agtccgacgt ctccatcgag cctctggtgg cctacgcggc cagcttcgtc
     1021 ccggggtatg ccgtgaagct cgtcctcacc aggaaagaag tcttcacaag cgccctggtg
     1081 ggcaggggca tgaagggacg aatgaagatc ggcgcgaaga aggacggaac cttcacggcg
     1141 ctgaaggcgg agatgtactt cgccgacggg gcctacggcg acacgggatg gcccgtggac
     1201 accgtggctg gtcacaactg caccggcccc tacgagttcc cccatgtcac ggtggacgtg
     1261 tacggaacat acaccaactc ccccatcgtg ggagcctacc ggggatacgg ccatcccgaa
     1321 gggcagctca tgtcgggccg cctgatcgac aggctggccc ggaagctcgg catgacaccc
     1381 ggggagatca tgcggaagaa cttcctgtcc gaagggcggc agaacgccct cgggcaggtc
     1441 ataaagaaga gccacggaga ccttcccggc tgcctcgccg ccgttgagaa ggccctggac
     1501 gagacccccc tcccgaaaaa cgacgaccgg ttcctctacg gcaggggcat cgcctccatg
     1561 atgaaatcgc ccaagatggc ggccaatgcc gcctccacct gcaccgtcca ggtcaacgcc
     1621 gacgggagcg ccttcgtgaa cctctcgggg atcgagatgg gccagggcgt ccagaccgtc
     1681 ttcgcccaga tggcggccga agccctgcag atccccatgg aaaaggtgaa gtcctatccc
     1741 gacgtggaca cccagttctc tccctgggag tggcagaccg tggcctccat gcagacctac
     1801 cgtggcgggc gggccatcca ggacgcctgc gggcagatca tcgacatcgc cagggagacg
     1861 gcggcgaagg tatggaactg ctctccgtcc ctggtcgcct
//
LOCUS       DAOTHD010000439         1898 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_72051_length_1898_cov_3.539338, whole genome shotgun sequence.
ACCESSION   DAOTHD010000439 DAOTHD010000000
VERSION     DAOTHD010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72051_length_1898_cov_3.539338"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="P5219_07740"
     CDS             complement(<1..238)
                     /locus_tag="P5219_07740"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HRX26688.1"
                     /translation="MAWRDMGIRKKLALGFGAVVLIAVILGAAGISGISRLSDDMAYV
                     GETRLPDILSFSRLNYLRMAIRAESLEGLRAEDSS"
     gene            complement(261..803)
                     /locus_tag="P5219_07745"
     CDS             complement(261..803)
                     /locus_tag="P5219_07745"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRX26689.1"
                     /translation="MNVNRQETPAERKARIFRERAKKLAAPGAPRDSGGESLELLEFL
                     IGKERYAVETVFVGEVFPPGDITPVPCTPPFVAGVVNLRGRIVPVVDLKVLFGIRSVG
                     EKRMSRIVLLQSGGAEIGLLTDGVQGIEITGREAVRPPLSAQGGLPGAYMTGIAGNDL
                     AVLDGDRILRDIALTVDEEP"
     gene            complement(800..>1898)
                     /locus_tag="P5219_07750"
     CDS             complement(800..>1898)
                     /locus_tag="P5219_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HRX26690.1"
                     /translation="YTLSMLLLNALPGMDPEQVSVLGTDINPFALQKARKGIFRRWSF
                     RGMAPEEIRLFFNDLGDGSFSVRERYRRTTAFSLLNLAERPWRLWRDGGPPDAVFCRN
                     VLIYFSPKKREEVLAGFHSLLPEGGWLAVAACETVPLQASGFSPVSSGGVTLYRKNGG
                     TAPVFFPEPFRSSDYSSSGEGEERVLSFEDFFSGGAAAPEEEYPLFAGNHGGEEADGS
                     GEPPAENAPDDSLEKARSLADRGFREDALEELRRVIGRDRTDPLPFYLLALIHQELGN
                     EKEAAAALRNTLFLAPGFVMAHYTLGSAALRKGSAKEADRHFRNAADLLLRLPGDSPV
                     PESGGLTAAHLLETVRELRSGTNNMTGADGK"
BASE COUNT          356 a          600 c          569 g          373 t
ORIGIN      
        1 aagagctgtc ctccgcccgc agaccttcca ggctttccgc cctgatggcc atccgcaggt
       61 aattcagccg ggaaaaggac agaatgtcgg gaagcctcgt ctccccaaca taggccatgt
      121 cgtccgaaag gcgggaaatt cctgagattc ccgccgctcc gagtatgacg gcgatgagta
      181 ccaccgcccc gaagccgagg gcgagttttt ttcgtattcc catatctctc catgccacaa
      241 tgaacccctc cttgcgggtt ttatggttct tcatcgacgg tcagggcgat gtccctgaga
      301 attctgtccc cgtcgagcac tgcaaggtca tttccggcga ttcccgtcat ataggctccg
      361 ggaagccccc cctgcgctga aaggggtggg cggacggctt cccttccggt aatttcgatt
      421 ccctgcacgc cgtcggtgag cagccctatt tcagccccgc ctgactggag cagcacaatc
      481 ctggacatcc tcttctcccc gacggaccgg atcccgaaga gcaccttcag gtccaccacg
      541 ggaacgatcc tgccccgcag gttgaccact ccggccacaa aggggggagt gcacggaacg
      601 ggggtaatgt cccccggagg gaagacttcg cccacaaaga ccgtttccac ggcgtatcgt
      661 tcttttccaa tgaggaattc gagcagttcc aggctttcgc cgcccgaatc ccggggcgct
      721 cccggggcgg cgagtttttt cgccctttca cggaatatcc tggcctttct ctccgcaggg
      781 gtttcctgtc ggttgacgtt cattttccat cagctccggt catgttgttt gtgccgctcc
      841 gcagttccct gaccgtttca agaaggtgcg ccgcagtcag tcctccgctt tccggaaccg
      901 gggaatcccc ggggagccgg agcagaaggt ccgcggcgtt ccggaaatgc ctgtccgcct
      961 cttttgcaga gcccttccgc agagccgcgg aacccagggt gtaatgggcc atgacgaacc
     1021 cgggggcaag aaacagggtg ttcctgagcg ccgcggcggc ctccttctca tttccgagct
     1081 cctggtggat gagagcgagg agatagaagg gaagggggtc cgtcctgtcc ctgccgatga
     1141 ccctccggag ctcttcgagg gcgtcctccc ggaaccccct gtccgcgagg cttcgggctt
     1201 tttcgaggct gtcgtccggg gcattttccg ccgggggttc tccagagccg tccgcttctt
     1261 ctccgccgtg atttcctgcg aacaggggat actcttcttc cggtgccgcg gcccctccgg
     1321 agaaaaaatc ctcgaaggac agaacacgct cctccccttc gcctgaggag gaataatccg
     1381 aagagcggaa gggctccgga aaaaataccg gggctgttcc gccgtttttt ctgtacaggg
     1441 tgaccccgcc ggaagaaacg ggggagaaac cggaggcctg gaggggcacc gtttcgcagg
     1501 cggcgaccgc aagccatcct ccctcgggca ggagggagtg gaaccctgcc aggacctcct
     1561 cccgcttctt cggggaaaaa tagatgagaa cgttgcggca gaaaaccgcg tccggagggc
     1621 ccccgtcccg ccagagccgc cacgggcgct ccgccaggtt gagaagggaa aaggcggtgg
     1681 ttctccggta ccgctccctg acggaaaagg atccgtctcc caggtcgttg aaaaacagcc
     1741 ggatctcctc aggggccatt ccccggaagg accaccggcg gaaaatcccc ttccgcgcct
     1801 tctggagggc aaaagggttg atgtccgttc cgagaacgga tacctgctcc gggtccattc
     1861 cgggaagagc gttcaggaga agcatggaga gggtgtag
//
LOCUS       DAOTHD010000440         1897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_72095_length_1897_cov_3.947340, whole genome shotgun sequence.
ACCESSION   DAOTHD010000440 DAOTHD010000000
VERSION     DAOTHD010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72095_length_1897_cov_3.947340"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..580
                     /locus_tag="P5219_07755"
     CDS             182..580
                     /locus_tag="P5219_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26691.1"
                     /translation="MRGAGLLTGFFLLSDGLRRLLTGGGGLSDFVPYLLVGSACVYVA
                     GYDKKVTLSEKGFVRQTLFWGKGRTDILPWSEMEEMILMPAGKGTGVLFPLKDRGWRA
                     FFPEATEADLRAAAAEYRPELPVSTGTGSR"
     gene            658..1761
                     /gene="tal"
                     /locus_tag="P5219_07760"
     CDS             658..1761
                     /gene="tal"
                     /locus_tag="P5219_07760"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HRX26692.1"
                     /translation="MTQDNTIYRALALGQSIWCDFLSRDLLRSGKLDKMIEDGVRGVT
                     TNPSIFENAIGKTSDYDGDILSMVRQGKKREEIYTSLTVADVREAADRFFPIYSGSGG
                     RDGFVSLEVSPLLAHDAEGTVSEAETLARLTGKKNVMIKIPATPEGMKAIRRCIALGI
                     NVNATLIFSGEQYRQVAEAFISGLEDRVSDGNPISGIASVASLFVSRVDTAVDKLLPS
                     GNSGAALAGKIAVDNARAAYMDFESLFSSPRWTVLAEKGAQVQRPLWASTGTKNPDYS
                     PTLYVDSLIGPHTVNTIPPATLEAFLAAGTVGMTVASDREGMKRRLDELEALGISLDE
                     VTGKLLKEGLDAFEKAYILLLDCIEKKGQSLAG"
BASE COUNT          412 a          504 c          548 g          433 t
ORIGIN      
        1 taccgaccct ttcatacctt ccctatgcct tcctcaacta tatcaaccct ctcgttgcgc
       61 ttctcatgac caggctggga attggaattc cctgggttga aaagcacgag accggagagg
      121 tcaccgcaga ttgagcgaaa cgagggtctt ttccggaagc ggacttcctt ttccgcggtg
      181 gatgaggggt gcaggtctgc tgaccggttt ctttcttctt tccgacggcc ttcgccgcct
      241 cctcacgggg ggcggcgggc tgtcggactt tgttccttac ctcctggtag gttccgcctg
      301 cgtttacgtg gccggatacg acaaaaaagt taccctttcc gagaaggggt ttgttaggca
      361 aaccctcttc tggggtaagg gaaggactga cattctcccc tggagcgaaa tggaagaaat
      421 gatcctcatg cctgcgggaa aaggaaccgg tgttttgttt cctctgaagg acaggggatg
      481 gagggcattt ttccccgaag ccacggaagc ggaccttcgt gccgctgccg cggaatacag
      541 accggaactt cctgtttcaa cgggcaccgg atcccgttaa atatataccc ttccaagggc
      601 ggcggcatcc gcgaagtgat tcaggttcac atctgaagag gagaggactt tttcaagatg
      661 acacaggata ataccattta cagggcgctt gctctcgggc agagcatttg gtgcgacttc
      721 ctcagcagag accttctccg gtccggcaag ctggacaaaa tgattgaaga tggtgttcgt
      781 ggagtgacga caaatccctc cattttcgag aatgccatcg ggaagacttc cgattacgat
      841 ggggacattc tttccatggt ccggcaggga aagaaaaggg aagaaattta tacgtcactg
      901 accgttgccg atgtccgtga ggcggcagac cggttcttcc cgatctatag cgggagcggc
      961 ggcagggatg gtttcgtgag tcttgaagtc agccctcttc tcgcccacga tgcggaaggg
     1021 accgtatcgg aggcggagac ccttgcccgg cttacgggga agaaaaacgt catgatcaag
     1081 attccggcga ctcctgaggg catgaaggca attcgccggt gtatcgccct gggcatcaac
     1141 gtgaacgcta cgcttatttt ttcgggagag caatacaggc aagtggcgga ggcgttcata
     1201 tccggccttg aagaccgggt gtccgatgga aatccgattt ccggaattgc ctccgtggct
     1261 tctcttttcg taagccgtgt cgacacagcg gtggacaagc tcctcccatc aggaaacagc
     1321 ggtgcggccc tcgccggcaa aatcgccgtg gacaatgcac gggcagccta catggacttt
     1381 gagtccctct tttccagccc ccgctggacc gtccttgcgg aaaagggagc ccaggtacag
     1441 cggcctctct gggcaagcac gggcacaaag aatccggact attcgccgac cctctacgtg
     1501 gattccctca tcggtcccca tacggtcaac acgatccctc cggcgactct cgaggctttt
     1561 cttgccgccg gtacagtcgg aatgactgtc gcctcggaca gggaaggaat gaagcgcagg
     1621 ctcgatgagc ttgaagctct cggcatctcc ctcgacgagg tgacggggaa gctgctcaaa
     1681 gaggggctgg atgcctttga aaaagcctat atccttctgc ttgattgtat agagaaaaaa
     1741 gggcagtcac tggctggctg atttttttct tcaaatcggg ttgacagaat cggcggggct
     1801 ccttataata ctcccgttgc gcgtaagggg ctatagctca gctgggagag cgcttgaatg
     1861 gcattcaaga ggtcaggggt tcgaatcccc ttagctc
//
LOCUS       DAOTHD010000441         1896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_72177_length_1896_cov_2.602390, whole genome shotgun sequence.
ACCESSION   DAOTHD010000441 DAOTHD010000000
VERSION     DAOTHD010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72177_length_1896_cov_2.602390"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="P5219_07765"
     CDS             <1..296
                     /locus_tag="P5219_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26693.1"
                     /translation="VVEAAAMLLRRMNLRADTFGGVITLPGCSGAGLLEAAGRTLDKS
                     LPSGEDLAADVPDTEIEKFDYLVPERLRRRGWAARYLDVPGAAEWLGSLAGRK"
     gene            344..1393
                     /locus_tag="P5219_07770"
     CDS             344..1393
                     /locus_tag="P5219_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HRX26694.1"
                     /translation="MDFRKRITDYGIKPGILPRGPRNAITDVEGVRVGHCTLADGPVQ
                     TGVTAVLPHGGNLFREKLLAACHVINGFGKSAGLVQVEEMGTLETPVILTNTFAVGAA
                     SEALVRYMLADNPDIGRETGTVNPVVCECNDGFLNDIRGFAVREEHVLRALASASEDF
                     EEGAVGAGRGMSCHQLKGGIGTASRVLEAEGASFTLGVLVLSNYGQLEDLRVDGRHIG
                     PAIGKIHPPQDEQGSIIAVIATDIPLTERQLRRVARRATVGITRTGAFIGSGSGEIAL
                     AFTTANRFRHHETAPLVTLSMLNENHINLAFRAAAEATEEAVLNSMAGAEPVSGRDGH
                     IRHSLAEYAHLFARQ"
     gene            1423..>1896
                     /locus_tag="P5219_07775"
     CDS             1423..>1896
                     /locus_tag="P5219_07775"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HRX26695.1"
                     /translation="MNSGTDFLTRAAELHRNSFVADAHFDLLPFVLEKREGGETRVIE
                     RHYLPGLREAGVDLVVSSLFLSGRYLPEMGLRRSLDYISALHAEMEESPGLFSLCRST
                     GEIRAANGRGELAVLLSFEGLDPIGNDLSLLRIFFELGVRGVGITWSRRNYAADGC"
BASE COUNT          340 a          635 c          620 g          301 t
ORIGIN      
        1 aggtcgtcga ggcggccgcc atgctgctcc ggaggatgaa cctccgggcg gacactttcg
       61 gcggcgtgat cacccttccc ggatgcagcg gtgccgggct cctggaagcc gcgggacgga
      121 ccctcgacaa gtccctgcct tccggcgaag acctggcggc ggatgttccg gacacggaga
      181 tcgaaaagtt cgactatctc gtcccggaac ggctccgacg gcggggttgg gccgcccggt
      241 acctggacgt cccgggcgcc gccgaatggc tcgggagcct ggccgggcgg aaatgataga
      301 atgaagccgt gatcccgtac gaaatcccca aggagatgaa ttcatggact tccgcaagcg
      361 cataaccgac tacggcatca aaccaggaat actgccccgg ggaccacgga acgccatcac
      421 tgacgtggag ggggtccggg tgggccactg caccctggcc gacggccccg tgcagacggg
      481 cgtcaccgcc gttctgcccc acggaggcaa cctcttccgg gaaaagcttc tcgcagcctg
      541 ccacgtgatc aacggcttcg gaaagtccgc cgggcttgtc caggtggagg agatgggaac
      601 gctggaaaca cccgtgatcc tcaccaacac ctttgccgtg ggagcggctt ccgaagccct
      661 cgtccggtac atgcttgcgg acaaccccga catcggccgg gagacgggaa cggtcaatcc
      721 ggtggtctgc gagtgcaacg acggcttcct caacgacata cggggcttcg ccgtccggga
      781 ggagcacgtg ctccgggccc ttgcctcggc ctcggaggac tttgaggagg gggccgtcgg
      841 ggcgggcagg ggcatgtcgt gccaccagct caagggcggc atcggcaccg cctcacgggt
      901 tctcgaggca gaaggggcct ccttcaccct gggagtcctc gtcctctcca actacggcca
      961 gctggaggac ctccgggtgg acgggcggca catcggcccc gccatcggga aaatccaccc
     1021 gccccaggac gagcaggggt ccatcatcgc cgtgatcgcc acggacatcc ccctcacgga
     1081 gcgccagctc cgccgggtcg cccgcagggc gaccgtgggc atcaccagga ccggggcctt
     1141 catcggcagc ggcagcgggg aaatcgccct cgccttcacc acggcgaacc gcttccgcca
     1201 ccacgaaacg gcgcccctgg tcaccctctc catgctcaac gaaaaccata tcaacctcgc
     1261 cttccgtgcc gccgctgagg ccacggagga ggcggtgctc aactccatgg caggtgcgga
     1321 acccgtctcc ggaagggacg ggcatatcag gcactccctg gcggagtacg cccatctctt
     1381 cgcccggcag tgacgggctt catcgaaaag gagaatgatt ccatgaacag cggaacggat
     1441 tttctgaccc gggcggcgga gctccacagg aactctttcg tggcggacgc ccacttcgac
     1501 ctcctgccct tcgtgctgga aaagcgggag gggggagaga cgcgggtcat cgagcggcac
     1561 taccttcccg gtctccggga ggcgggtgtg gacctggtgg tgtcttccct gttcctgagc
     1621 ggccggtacc ttcccgagat gggtctccgc aggtctctcg actacatcag cgccctccac
     1681 gccgaaatgg aggaaagccc ggggctcttc tccctctgcc ggagcaccgg cgagatcagg
     1741 gcggcaaacg gacggggaga gcttgccgtc ctcctttcct tcgaggggct tgaccccata
     1801 gggaacgatc tctccctcct caggatcttt ttcgagctcg gcgtccgggg tgtgggcatc
     1861 acctggagca ggaggaacta cgccgccgac gggtgc
//
LOCUS       DAOTHD010000442         1894 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_72257_length_1894_cov_3.497009, whole genome shotgun sequence.
ACCESSION   DAOTHD010000442 DAOTHD010000000
VERSION     DAOTHD010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72257_length_1894_cov_3.497009"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="P5219_07780"
     CDS             <1..644
                     /locus_tag="P5219_07780"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300810.1"
                     /note="Catalyzes first step of the de novo purine
                     nucleotide biosynthetic pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRX26696.1"
                     /translation="VYFARPDSVISGRSVYEVRKEQGRKLADRAPCPEAHLATGMPDS
                     GTIAALGYAEKSATPYEQTVVRNRYVGRTFIEPTQRVRELGVRIKLNPITEVLSGKNV
                     VVVDDSIVRGTTCQRMISMIKACGANQVHVRISSPPVRFPCYYGIDTPTRVELAAARM
                     NLRQLEREVGADSLSYITEEDLIEAIGLPCGEVCTACFSGQYMEGGEDHEPEL"
     gene            628..1626
                     /gene="purM"
                     /locus_tag="P5219_07785"
     CDS             628..1626
                     /gene="purM"
                     /locus_tag="P5219_07785"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRX26697.1"
                     /translation="MNLNYRDAGVDIPLADQWVGSIRNIVRSMPADPNVLGGIGGFSG
                     LYRIPGGLVLAGCCDGVGTKIEVAKAAGKFDGIGQDLVAMNVNDLVTCGARPLFFLDY
                     IACGRLNPGILAPIVESAARACRESGCALLGGETAEMPGTYNEDGLDLAGFSVGMLRE
                     EEIIDGSRIATGDAVVGISSSGVHSNGFSLVRKALFSSENPCGLSDTPEVLEGKTLGE
                     SLLVPTRLYVSVALEAARSGLVGGMAHITGGGLYDNIRRVVPENLSLSIHYGAWPRPP
                     IFSLLRDRGIDESEMRKVFNLGIGFAFIVRKENLPEFLSLLEGLGEKGYVIGGVSA"
     gene            1623..>1894
                     /locus_tag="P5219_07790"
     CDS             1623..>1894
                     /locus_tag="P5219_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HRX26698.1"
                     /translation="MTSFAVLISGRGTNMAAIARAVAGNRLKARLKFVASDNPGAAGL
                     DVARKFGYRAEILDYEKEGRSGAERQLVRLCSLNKVEWIVLAGFMR"
BASE COUNT          389 a          558 c          598 g          349 t
ORIGIN      
        1 tcgtctattt tgccaggccc gacagcgtca tcagcgggcg ctcggtctac gaagtcagga
       61 aagagcaggg aaggaaactt gccgaccggg ccccctgccc cgaagcccac ctcgccacgg
      121 ggatgccgga cagcggcacc atcgcagccc tgggctacgc cgaaaaaagc gccactccat
      181 acgaacagac cgtagtgagg aacaggtacg tcggcaggac cttcatcgaa ccgacccagc
      241 gggtcaggga actcggcgtg agaatcaaac tcaaccccat aacggaagta ctgtcgggga
      301 agaacgttgt cgtggtggac gactcaatcg tccgcggaac cacatgccag aggatgatct
      361 ccatgataaa ggcatgcggc gcaaaccagg tccatgtcag gatttcctct ccgccggtcc
      421 ggttcccgtg ctattacggc atcgatacgc cgacgagggt ggaacttgcc gcggcccgga
      481 tgaacctccg gcagctcgaa cgggaggtgg gcgcggattc cctctcctac atcaccgagg
      541 aggacctcat cgaggcgatc ggccttccct gcggcgaggt ctgcacggcc tgcttttccg
      601 gccagtacat ggagggagga gaagatcatg aacctgaact atagggacgc gggagtggac
      661 atccctcttg cggaccaatg ggtagggagc atcaggaata tcgtccggtc catgccggcc
      721 gatccgaacg tgctgggtgg tataggcggc ttcagcgggc tgtacagaat tcccggcggg
      781 ctcgtcctcg cggggtgctg cgacggcgtg ggaacgaaaa tcgaagtggc caaggcagcg
      841 ggaaagttcg acgggatcgg ccaggacctg gtggccatga acgtgaacga tcttgtcacg
      901 tgcggcgccc ggcctctctt cttcctcgac tacatcgcct gcggaaggct gaatcccggg
      961 attctcgccc ctatcgtgga aagcgcggcc cgggcctgcc gggagagcgg atgcgccctc
     1021 ctcggcgggg agacagcgga aatgccgggt acctacaacg aagacgggct ggatcttgcg
     1081 gggttttccg tcggcatgct ccgggaagag gaaataatcg acggaagcag gatcgccacc
     1141 ggcgacgccg tcgtcggcat ttcgagttca ggagtgcaca gcaacgggtt ttccctcgtc
     1201 cggaaggccc ttttctcttc cgaaaaccct tgtggtcttt ccgacacgcc ggaagtgctg
     1261 gagggaaaaa cactcggcga gagcctcctt gttccaacca ggctgtatgt ctccgtcgcc
     1321 ctcgaggccg cacggtccgg gctggtgggg ggcatggcac acatcaccgg cggggggctc
     1381 tatgacaaca tccgccgggt cgtccccgaa aacctttccc tgtcaataca ttacggtgcc
     1441 tggccccgtc ctcccatttt ctcccttctc cgggaccggg gaatcgacga gagcgaaatg
     1501 cgcaaagtct tcaatctggg catcggtttt gcattcatcg tcaggaaaga gaatctcccg
     1561 gagtttctct cccttcttga aggtctggga gaaaaggggt atgtcatcgg cggggtctcg
     1621 gcatgacatc ctttgccgtc ctcatctccg gacgggggac gaacatggcg gccatcgccc
     1681 gggccgtggc cggaaacagg ctgaaagccc gcctgaagtt tgtcgcgagc gacaaccccg
     1741 gtgcggccgg acttgatgtt gcaagaaaat tcgggtaccg ggcggaaatt ctcgattacg
     1801 aaaaggaagg cagatcaggg gcggaacggc agcttgtccg cctctgctcc ctgaataaag
     1861 ttgagtggat tgtcctcgcc ggcttcatgc gcat
//
LOCUS       DAOTHD010000443         1894 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_72259_length_1894_cov_3.360522, whole genome shotgun sequence.
ACCESSION   DAOTHD010000443 DAOTHD010000000
VERSION     DAOTHD010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72259_length_1894_cov_3.360522"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..758)
                     /locus_tag="P5219_07795"
     CDS             complement(177..758)
                     /locus_tag="P5219_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26699.1"
                     /translation="MNIMPLFPERLQDFCAPAPRPGEYLLERRFAEIYAAARGIPLKY
                     DELLEEIRQWCEASGIGGHGGSVSFSGRRGGREYRGTATRFRDELSILIHAEGEGRRR
                     YRIPGLWSDYSWLVLYQEPLSGEWRSWPGAAKEPSAMERDRTTEEKAGEGFDWVCRRR
                     IISRVRLFRGECLVKEYFARPEKTGAGEPPGPP"
     gene            898..>1894
                     /locus_tag="P5219_07800"
     CDS             898..>1894
                     /locus_tag="P5219_07800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRX26700.1"
                     /translation="MAQSQDRTHFMGTDSIPRLMIRFSLPAIGGMLANALYNIVDRIF
                     VGRTVGPAGIGAISVAFPFMLLVIAYGLLIGVGAGALVSISLGEKRRARAEKTMGNAL
                     LFLAGGSIVIAAAGGMLSTPVLELSGASPTMLPLSKEYLDIIVWGIPFSTLAFGLNYF
                     IRAEGNPRYAMYTLFVGAFANIILDAVFILKLGMGIRGAALATVASQLLSLGWAAAYY
                     LRRRGVLRFRRKNLRPDRVIVGRMLAIGTAPFLTEFSFTFIMALFNRFLRTYGGDLAI
                     SAMGIFFSLDSLLFLPVLGLAEGVQPLIGYNYGAKDYGRVIAAVRSAILMGLAFFA"
BASE COUNT          305 a          613 c          571 g          405 t
ORIGIN      
        1 cggacgaaaa gtacaggcgg gagttcttcg cccggttcag ggaccgtctc gctttcgtga
       61 cgtcgaggga gacggagaaa ggcggcatgg acaaactccc gcccgaggcc gaagccgctt
      121 tgcggaaggc cctggacgag atcggctacc cggtgcagga ctaggcggaa aggaagctat
      181 ggcgggccgg ggggttcccc ggcccccgtt ttttccgggc gggcgaaata ttccttcacc
      241 aggcattccc cccggaaaag gcggactctt gaaatgatcc gccttctgca gacccagtca
      301 aacccctccc cggctttttc ttccgttgtc ctgtcccgtt ccatggcgga gggctctttc
      361 gcggcccccg gccatgagcg ccactcgccc gacaggggct cctggtacag gacgagccag
      421 gaatagtccg accagagccc cggaatgcgg tagcgcctcc gtccttcccc ttcggcgtgg
      481 atcaggatgc tcagttcgtc ccggaacctg gtggcggtcc cccggtattc cctgcccccg
      541 cggcggccgc tgaaagagac gcttccgccg tggccgccga tgccggaggc ttcgcaccac
      601 tgccgtatct cctccagcaa ttcgtcgtac ttcaggggaa ttccccgggc ggcggcatag
      661 atttcggcga agcgccgctc caggagatat tcgcccggcc tgggagcggg ggcgcagaag
      721 tcctggagcc gttcggggaa aaggggcatg atgttcatgg ggtctccttc ggataaacgt
      781 gccggtccaa aaccgggggc atcttgccgg gtccggcgtt ttctgtttcg gctttactat
      841 attattcttt tccctataat catactcctg acggacatca cttcggaggc ttcgattatg
      901 gcacaatcgc aggacagaac gcactttatg gggaccgatt ccattccccg cctgatgatc
      961 cggttttccc tgccggccat cggcgggatg ctggccaacg ccctgtacaa catcgtggac
     1021 cgcatcttcg tcgggcgcac cgtggggccg gcgggaatcg gcgccatcag cgtggcgttt
     1081 cccttcatgc tgctggtcat cgcttacggg ctgctcatcg gtgtaggggc tggagccctg
     1141 gtgtccattt ccctcggcga aaagcggcgt gcccgggctg aaaaaaccat ggggaacgca
     1201 ctccttttcc tcgcgggggg cagcattgtc atcgctgcgg cggggggaat gctcagcacc
     1261 ccggtcctgg agctttcggg cgcgagtccc accatgctgc ccctgtcaaa ggaatatctc
     1321 gacatcatcg tctggggcat ccctttttcg acccttgcct tcgggctcaa ctatttcatc
     1381 cgggcggagg ggaatccccg ctatgccatg tacaccctct tcgtgggcgc cttcgccaac
     1441 atcatcctgg acgctgtctt cattctgaag ctcggcatgg gcatcagggg tgcggccctg
     1501 gccactgtgg cttcccagct cctctccctg gggtgggccg ccgcctatta ccttcgccga
     1561 cggggcgttc tccggttccg gagaaaaaat ctccgtcccg acagggtgat cgtcggaagg
     1621 atgctggcca tcgggaccgc cccgttcctg acggaattca gcttcacctt catcatggcg
     1681 ctcttcaacc gtttcctccg gacctacggg ggagatcttg ccatctccgc catgggaatc
     1741 ttcttcagcc tcgacagcct ccttttcctc ccggtcctcg ggcttgcgga gggagtgcag
     1801 cccctcatcg ggtacaacta cggcgcgaag gactacggcc gggtgattgc cgccgtccgt
     1861 tccgccatac tgatggggct cgctttcttc gcgg
//
LOCUS       DAOTHD010000444         1890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_72477_length_1890_cov_2.649046, whole genome shotgun sequence.
ACCESSION   DAOTHD010000444 DAOTHD010000000
VERSION     DAOTHD010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72477_length_1890_cov_2.649046"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..826
                     /locus_tag="P5219_07805"
     CDS             113..826
                     /locus_tag="P5219_07805"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HRX26701.1"
                     /translation="MKEMYYTIADEVFTAFPDYVRGVVVARDVRNGPSPGELTALLRE
                     AEAALRETLGTGNPAEHPRIASWREAFRKTGVKPAEFRSSIEAMARRVAKGQELPSIN
                     ALVDIGNVLSLRHLVPTGGHSLDDVTEDIALRAATGNEVFVPFGETEEEHPFPGEFIF
                     AEGKKVLTRRWSWRQANHTLTLPETKSIEFNVDGLPPVERTEVEAICGELEELVGRFC
                     GGTFRREILCASQPRMSLS"
     gene            complement(1466..>1890)
                     /locus_tag="P5219_07810"
     CDS             complement(1466..>1890)
                     /locus_tag="P5219_07810"
                     /inference="COORDINATES: protein motif:HMM:NF024898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRX26702.1"
                     /translation="FDGMNFDTRMKKSWESALEKHIAIAKDGALPVGYVFSTVESAEG
                     MKNSSFRLLPDTAGFPERIGCLSNLYIRDGYRGSGLGSRLFDISMEWLESFPYMELIY
                     IFISNGNGEAMDFYLKHGFSYSHDVLGGFIQAVSKRVK"
BASE COUNT          389 a          572 c          566 g          363 t
ORIGIN      
        1 ggccaaggaa gagctccggc tccggctgga cggcgacagc taccgctgcg ccattcccga
       61 cgacgtccgg cccaggcccc tgaattcagg cagataggaa cggaggctct ttatgaagga
      121 aatgtattac accatagcgg acgaagtgtt tacagccttt ccggactatg tccggggcgt
      181 ggtggttgcc cgggacgtgc ggaacggccc ttcccccggc gaactgaccg ccctgctgcg
      241 ggaggccgag gcggccctga gggagaccct cgggacgggc aatcctgcgg agcacccccg
      301 catcgcctca tggcgtgagg ccttccggaa gacgggcgtg aagccggcgg aattccgttc
      361 ctccatcgag gccatggccc ggcgggtcgc gaagggacag gagcttcctt ccatcaacgc
      421 cctggtggac atcggcaacg tgctgtccct gcgccatctc gtccccacgg gaggtcactc
      481 cctggacgat gtgacggagg acatcgccct ccgggcggcc accgggaacg aggtgtttgt
      541 ccccttcggt gaaacggagg aggagcatcc cttcccaggc gagttcattt tcgccgaggg
      601 aaagaaggtg cttacgaggc gctggagctg gcgccaggcg aaccacaccc tcacccttcc
      661 ggagaccaag tccatcgagt tcaacgtgga cggcctgcct cccgtggaga gaaccgaagt
      721 ggaagcaatc tgcggcgaac ttgaggaact ggtggggcgc ttctgcggcg ggaccttccg
      781 ccgggagatc ctgtgcgctt cacagccccg gatgagcctg tcgtgattct gcctttagca
      841 tcattctgag ggcaaagccg gtttgtcatc ctgagcgcga agttagtttg tcatcctgag
      901 cgcgaagtta gtttgtcatt ctgagggcga agcccgaaga atcccgcccc ttggaacctt
      961 agaatcaaac ccagatcctt cccctccggg gacgcgatcg tcgcaaaaac cgctcctcgg
     1021 aatgacaggg ggcttgtttg tcatcccgag cggagcgatc gcccagtatc cccggagggg
     1081 gaggatctcg cccctggtgg tccaggaatt aaacccgaga tccttcgggc aaaaaacgcc
     1141 ctcaggatga caaacaaagc gcatgttcgg gccgatgctg cccttgtttg tcatcccgag
     1201 cggacgcgag ggatctcggt cttgagcagc tggaatcaaa agcgagattc ctcccctccg
     1261 gggacgcgat cgtcgcaaaa accgctcctc ggaatgacag aaaacacgct gtgttgaggc
     1321 gtaccctcag gacgacagaa tcgagatcct tcgtcgccaa aaccgctcct caggatgaca
     1381 aaacgatagt gtcatcctga gggcaaagcc ggtctgtcat cctgaggagc gcagcgacga
     1441 aggatctgca gttggttttc agggctcatt tcacccgttt gctcaccgcc tggatgaacc
     1501 ccccgagcac gtcgtggctg taggaaaacc cgtgtttcag gtagaaatcc atggcctcgc
     1561 cgtttccgtt ggagatgaag atgtagatca gttccatgta cggaaagctc tcaagccact
     1621 ccatggagat gtcgaaaagc cgggagccca gtcccgagcc ccggtagccg tcccggatgt
     1681 acaggttgct gaggcacccg atccgttccg gaaaaccggc cgtatccggc aggagcctga
     1741 aggacgagtt cttcatcccc tccgccgatt ccacggtgga aaagacgtat cccacgggaa
     1801 gagccccgtc ttttgctatg gcaatgtgtt tttccagggc gctctcccag ctctttttca
     1861 tcctcgtgtc gaaattcatg ccgtcgaacc
//
LOCUS       DAOTHD010000445         1885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_72733_length_1885_cov_2.869945, whole genome shotgun sequence.
ACCESSION   DAOTHD010000445 DAOTHD010000000
VERSION     DAOTHD010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72733_length_1885_cov_2.869945"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..799
                     /locus_tag="P5219_07815"
     CDS             158..799
                     /locus_tag="P5219_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26703.1"
                     /translation="MKKAPAVLIALVLMFCCLPAGADAALPAGPAPDAERVETPEGIT
                     FITTYSARGSRSEARHHYLEIGGFPVPDVFSRLKYRDCSFVFSSRQHLWGDDGYVQTG
                     DLAVPESPSAVEEKQLEKGWYEGAERLSGTPENWIWGAWEGGSLFAAPEKLAEAAKEL
                     KLPVLPREGSPLYEALRPVPAEPDAEPPEKKTQDTVSGDEALKALEEALKEKK"
     gene            complement(806..1462)
                     /locus_tag="P5219_07820"
     CDS             complement(806..1462)
                     /locus_tag="P5219_07820"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRX26704.1"
                     /translation="MKKSAVCWAMLILAVIGAGAAPARPPLEKGNGRTVAITFDDGPR
                     PGFVEPVLDILREKGVKATFFVVGRYAAQHPHLVRAMAAGGHVVANHTFYHNNLTALP
                     RENVHREWRLCSETIESILGEKPRFARPPGGQYNAFVVDQAAAEGLRIVLWTNNPGDY
                     NDSLGAPELTGKVLAKAAPGDILLLHVGVGPTIGALPGIIDGYRRKGFSFVTVEDIAR
                     "
     gene            1672..>1885
                     /locus_tag="P5219_07825"
     CDS             1672..>1885
                     /locus_tag="P5219_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRX26705.1"
                     /translation="MLSFFDMSELKDGVTRQVYLTLRRDILNWRLLPGRRLSEKDIAG
                     RMNISKTPVREAFIKLQGEGLLEIRPQ"
BASE COUNT          382 a          580 c          563 g          360 t
ORIGIN      
        1 ccgcgggcat tgacgtctca tctgaacagg agcggctgtg ccggctggtg atccgctcgg
       61 aatgcgttac ccgctgcata tcggaatccg gagccccgga ccttgaagtc ctgtttcccc
      121 cggcggaggg atggaccatg gaaaaggagg gaatatgatg aagaaagcac cggcagtcct
      181 gatcgcactg gttctgatgt tctgctgcct ccctgcggga gcggatgccg cccttccggc
      241 cggccccgcc cctgatgcgg aacgggtgga gaccccggaa ggaatcacct tcatcaccac
      301 ctacagcgca agaggctccc ggtccgaggc ccggcaccac tatctcgaaa tcggcggatt
      361 tcccgtgcct gacgtgtttt cccggctgaa atacagggac tgttcttttg tattttcctc
      421 gagacaacat ctctgggggg atgacggata cgtccaaaca ggggatctcg ccgttcccga
      481 atccccgtcc gcagtggagg aaaagcagct tgagaagggg tggtacgaag gtgcggagcg
      541 cctttcagga acccctgaaa actggatctg gggcgcctgg gaagggggct cactctttgc
      601 cgcccctgaa aagctcgccg aggcggcgaa ggaactgaaa ctccccgtgc ttccaaggga
      661 aggctcccct ctctacgaag ctctccgccc ggtgccggcg gagcccgacg cagaaccgcc
      721 cgaaaagaag acacaggaca cggtctccgg ggacgaagcc ctgaaggcac tggaggaagc
      781 gctcaaggag aaaaaatagg accttctacc gggcgatgtc ctccacggtg acgaaggaaa
      841 agccttttct ccggtagccg tcgatgatcc ctgggagagc cccgatggtg ggacccactc
      901 ccacgtgaag cagcaggatg tcgcccgggg cggccttcgc cagcactttc ccggtaagtt
      961 ctggtgcgcc gagactgtcg ttgtaatcac ctggattgtt tgtccagaga acgatccgca
     1021 gcccctcggc ggctgcctgg tcaaccacga aggcgttgta ctgtcctccc ggcggccggg
     1081 caaaccgggg tttttccccg agaatggatt cgattgtttc gctgcagagc ctccattccc
     1141 ggtgaacatt ctcccggggc agggcggtga ggttgttgtg gtaaaaggta tggttggcca
     1201 ccacgtggcc gccggcagcc atggcccgga ccaggtgggg gtgctgtgcg gcgtaccggc
     1261 ccaccacgaa aaaggtggcc tttactccct tttccctcag tatgtccagt accggttcca
     1321 cgaatccggg cctgggaccg tcgtcgaaag ttatggcaac ggtccggccg tttcctttct
     1381 caagaggcgg ccgggccggg gccgcgccgg ccccgatcac cgcgagaatc agcattgccc
     1441 agcagactgc agatttcttc actttcttct ccccgctccc gcttccatct gcacaacggg
     1501 gcagatgata gcaaattccc cccgcttccg ccatggttcg cgaaattggc ggacatatct
     1561 tgacaaatga acgggacact tctaagatac taacatatca gtcgcctctt cgcgcttgcg
     1621 tcaattgcgc gcattttccg gcggaaacct tccacgaaag ggacgttgcc aatgctctcc
     1681 tttttcgaca tgagcgaact caaggacgga gtcacccggc aggtgtacct gaccctgaga
     1741 agggacatcc tcaactggcg cctccttccc gggagacggc tcagcgagaa ggacatcgcc
     1801 gggcggatga acatcagcaa gaccccggtc cgggaagcct tcatcaagct ccagggggaa
     1861 gggctccttg agatccgccc ccaga
//
LOCUS       DAOTHD010000446         1878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_73094_length_1878_cov_3.352715, whole genome shotgun sequence.
ACCESSION   DAOTHD010000446 DAOTHD010000000
VERSION     DAOTHD010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73094_length_1878_cov_3.352715"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..105)
                     /locus_tag="P5219_07830"
     CDS             complement(<1..105)
                     /locus_tag="P5219_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRX26706.1"
                     /translation="MTKERIGFIGLGIMGKRMALNLLKAGYSMTIRSGN"
     gene            complement(222..1805)
                     /locus_tag="P5219_07835"
     CDS             complement(222..1805)
                     /locus_tag="P5219_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26707.1"
                     /translation="MMKKLSCLVLGVAVLAAAVVGGRPSSAQTPVSADEIRFVMPSGW
                     VKKEAPAPGTLAYYVLMYQGKPYGEMYLSKEPLSSVLSARQVLEEGLRKNSAGLQGYQ
                     PMGTFPVSAAGVSDAVAHDFTYFPAQSPVPFTGRVVVLVVGNASYTFFFNTTSSFFPS
                     VKGSFGDVTASIQAVPKPPVQEVKKAEPRTVEEHGFTMTLAGGWSDSGDAMGAKYRYA
                     GADGHYLASFFPFGKDEKAGVTALFALMEKRDALEAVLASKVRDDFPGYGDYTAVDTF
                     RRKVAGSDAIIHDFRSRKENVQVMFRWCIVAVSAVPANETTLTAPSVYDFAFLTSHPD
                     RFGELKPVFDAILDSMKPSVPLPLRPEAPAVQEGTTPPSGAVGQGLPELGDEEVFSAP
                     DGRFTVALPPGAVKDGAPRPEFSAPSGEVATYGIAGKEKTVVILHLFNALPEGEAYRD
                     NLAAARGGRAAGDVVMDAGRKKVPVRIYQTRDGQVLVAALFPADGLFIGVQLPKEAYS
                     GSRGWIQDLVTGVRFREGD"
BASE COUNT          367 a          602 c          526 g          383 t
ORIGIN      
        1 gttcccgctg cggatggtca tggaataccc cgccttgagc aggttcagcg ccatccgctt
       61 ccccatgatc cccaggccga tgaatccgat ccgttctttc gtcatgccgt accgtccctt
      121 ccctgatgta gttcgcgctc cgcaccgtca ttgtatcatc agggaaaggc cgccgcgcaa
      181 tggatgcgga ggcggttcct tcggctatgg gcagggatcg gctaatcccc ctccctgaac
      241 cggactccgg tgacaagatc ctgtatccag cctctcgacc cgctgtaggc ctccttcgga
      301 agctgcaccc ctataaagag accgtcggcc gggaaaaggg cggccacaag aacctgcccg
      361 tcccgggtct ggtagatcct gaccgggacc tttttccggc ccgcgtccat gaccacatct
      421 ccagccgctc tcccgcccct ggccgccgcc agattgtctc tgtacgcctc tccttccggg
      481 agagcattga aaagatggag aatgacgacg gtcttttctt ttccggctat gccataggtg
      541 gcgacttctc ccgaaggggc agaaaattcc ggcctgggag ccccgtcctt aactgccccc
      601 gggggaagcg ccacggtgaa ccttccgtca ggggcactga acacctcctc atccccgagt
      661 tcgggaagtc cctggccgac cgctccggaa gggggtgtcg tcccttcctg gacagccggg
      721 gcctcaggcc ggagaggaag aggaactgaa ggcttcatgg aatcgaggat cgcgtcaaag
      781 accggtttca actccccgaa cctgtccgga tgggatgtca gaaaagcaaa atcgtaaacc
      841 gacggcgccg tcagtgtcgt ttcgttcgca ggaacggcac tgaccgcaac gatgcaccag
      901 cggaacatga cctggacgtt ttccttccgg gagcggaaat catggatgat cgcatcgctt
      961 ccggcgactt ttcgcctgaa tgtgtccact gcggtatagt cgccgtagcc cggaaaatca
     1021 tcccgcacct ttgaagcgag caccgcttcc agggcatccc ttttttccat gagcgcgaaa
     1081 agcgcggtta cccctgcctt ttcatccttg ccgaagggga aaaaggacgc caggtagtgt
     1141 ccgtccgctc ccgcataccg gtattttgcc cccatggcat ccccggagtc actccaccct
     1201 cccgcaaggg tcattgtgaa gccgtgttct tccaccgtcc ggggttcggc cttcttcacc
     1261 tcctgtacgg ggggcttcgg cactgcctga atggacgccg tgacgtcacc gaaggatccc
     1321 tttacggacg ggaaaaagct cgaagtcgtg ttgaaaaaga acgtgtagga ggcattgccg
     1381 accacgagga ccaccacccg gcctgtgaag ggaacgggac tttgggccgg gaagtaggtg
     1441 aaatcatggg ccacggcgtc actgactcct gccgctgaaa cgggaaaagt tcccatgggc
     1501 tgatagccct ggagacccgc ggagtttttc ctcagcccct cttcgagcac ctgccgggcg
     1561 ctgagcactg acgaaagggg ttccttcgag aggtacattt ctccgtaggg cttgccctgg
     1621 tacatcagca cgtaataggc cagcgttccg ggcgccgggg cctctttttt cacccagccg
     1681 gaaggcatga cgaaccggat ttcgtcagcc gacaccgggg tctgcgccga agagggcctt
     1741 ccgcccacta cggcggcagc cagtacagcc actcccaaaa ccaggcagga cagctttttc
     1801 atcattgcat ttcccttctt cctttttgcc gtctttcccc tattttccga acagcaggag
     1861 aagcggcgcc agcagcag
//
LOCUS       DAOTHD010000447         1878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_73098_length_1878_cov_3.176632, whole genome shotgun sequence.
ACCESSION   DAOTHD010000447 DAOTHD010000000
VERSION     DAOTHD010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73098_length_1878_cov_3.176632"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="P5219_07840"
     CDS             complement(<1..938)
                     /locus_tag="P5219_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRX26708.1"
                     /translation="MRAVVKYKREDGNTEVRDVPVPEIGPSDVLVKVAYIGICGSDPH
                     MFHNQVSYPMAVPIILGHEFSGTIEKVGSDVKNWKPGDRVTAETHARFCGKCVLCKTN
                     NYRFCRERKGYGFGVDGAFAEFVSVPERILHAVPDSVTLKDASCTEPVCVAYNVVIAK
                     MGVKAGDSVAVLGPGPIGILCIHMAKMAGASNIIVFGAPGDEKRLEIARAYGATETYV
                     LGGSVDPKTVAAKFNEGYGFDKVVDAAGPAATLKISMDVVMPGGIINKVAWGPKPVDM
                     SLDPLLSKGVTLQGSFSHTWDIWEKVLVMMGQGQMPL"
     gene            complement(987..>1878)
                     /locus_tag="P5219_07845"
     CDS             complement(987..>1878)
                     /locus_tag="P5219_07845"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRX26709.1"
                     /translation="VSEFLAVLQEEVAYKIEGKVGKVIPPMKEFVPLIPSAIRSGIIG
                     VIIGAIPGAGEDIGSWLSYDVGKRRSKKGHLFGTGSYEGLVCSEVANNAVIGGAMIPL
                     LTLAVPGSPPSAMFLAAVWLHGVRPGPMLNFENPGFLPLVAAMLILGSIAMLIWGLSL
                     AKPMVAVLKIKREILLPIVIPLTVIGAYAGGVRLFDVYLMLAFGVLGYVLRQMDYPLA
                     PLVLGLILGPLADTSFRRALQQSRGAIMPLLERPIGLILLACVAWLIWTGIRGTRRYY
                     AEKAIADAAEDAAAEAAEQN"
BASE COUNT          327 a          539 c          642 g          370 t
ORIGIN      
        1 aggggcatct gtccctggcc catcatgacg aggacctttt cccagatgtc ccaggtgtgg
       61 ctgaaggagc cctggagggt gacgcccttg gagagcaggg ggtcgaggct catgtccacc
      121 ggcttgggac cccaggcgac cttgttgatg atgccgccgg gcatgacgac gtccatggat
      181 attttcaggg ttgcggctgg tcccgccgcg tcgaccacct tgtcgaatcc gtagccttcg
      241 ttgaacttgg ccgccacggt cttgggatcc accgatccgc ccaggacgta ggtctctgtc
      301 gccccgtagg cccgggcgat ttcgaggcgc ttttcgtcac cgggagcgcc gaagacgatg
      361 atgttgctgg ctcccgccat tttggccatg tgaatgcaca ggatgccgat ggggccgggg
      421 ccgaggacgg ccacggagtc gccggccttg acgcccatct tggcgatgac cacgttgtag
      481 gccacgcaga cgggttcggt gcaggaggcg tccttgaggg tgactgagtc gggaacggcg
      541 tggaggatcc tctcgggaac ggagacgaat tcggcgaaag cgccgtcgac accgaagccg
      601 tatcccttgc gttccctgca gaaacggtag ttgttggtct tgcagaggac gcatttgccg
      661 cagaagcggg catgggtttc ggcggtgacc cgatcgccgg gcttccagtt ttttacgtcc
      721 gaaccgactt tttcgatggt ccccgagaat tcgtgcccga ggatgatggg caccgccatg
      781 gggtatgaaa cctggttgtg gaacatgtgg gggtcgcttc cgcagatgcc gatataggcc
      841 accttcacca ggacatcgga aggcccgatt tccggtacgg gaacgtcccg cacttccgtg
      901 ttgccgtctt cccttttgta tttcaccact gcgcgcatag tttctctctc ctcctcatga
      961 accggggtgt tctccggagc ggatcttcaa ttctgttcgg cggcttccgc agcggcgtct
     1021 tccgctgcat cggcgatcgc cttttctgca tagtatctcc tggttcccct gatgccggtc
     1081 cagatcagcc acgcgacgca ggccagcagg atgaggccga tgggccgttc gagcaggggc
     1141 attatggcgc cgcggctctg ctgcagggcc ctccggaagc ttgtgtcggc cagggggccg
     1201 aggatgagtc ccaggaccag cggagcgagg gggtagtcca tctggcgcag cacgtagccg
     1261 agcacgccga aggcgagcat gaggtagacg tcgaaaagcc tgacgccgcc ggcgtaggcg
     1321 ccgatcaccg tcaggggaat gacgatgggc agaaggattt cccgcttgat cttgagcacc
     1381 gccaccatag gcttggcgag tgacagcccc cagatgagca tggcgatgga tccgaggatg
     1441 agcatggcgg ccacgagggg caggaagccg gggttttcga agttgagcat ggggccgggc
     1501 cggacgccgt gaagccagac ggcggccagg aacatggccg acggggggct gccgggaacg
     1561 gcgagggtga gcagggggat catggcgccg ccgatgacgg cgttgttggc cacctcggag
     1621 cagacgaggc cttcatagct gccggtgccg aagagatgtc ccttcttgga gcgccgtttg
     1681 cccacgtcgt aggaaagcca gcttccgatg tcctctccgg ctccggggat ggcgccgatg
     1741 atgacgccga tgattcccga gcggatcgcc gaagggatga ggggaacgaa ttctttcatg
     1801 ggtgggatga ccttgccgac ttttccttcg atcttgtagg ccacttcctc ctggaggacg
     1861 gcgaggaact cggagacc
//
LOCUS       DAOTHD010000448         1877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_73160_length_1877_cov_2.344676, whole genome shotgun sequence.
ACCESSION   DAOTHD010000448 DAOTHD010000000
VERSION     DAOTHD010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73160_length_1877_cov_2.344676"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="P5219_07850"
     CDS             complement(<1..414)
                     /locus_tag="P5219_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HRX26710.1"
                     /translation="MADDVFFGQDKEPVFSHLDGEGRPRMVDVSGKPETLRTAVAEGW
                     VILDRAVCEQLSADGYSKKGDVLKIAETAGIMAVKKTPELIPLCHGIRIDRAEVKCYF
                     LEERGRIRISCTIAARDVTGVEMEALTGVSVGLLTI"
     gene            complement(418..1431)
                     /gene="moaA"
                     /locus_tag="P5219_07855"
     CDS             complement(418..1431)
                     /gene="moaA"
                     /locus_tag="P5219_07855"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HRX26711.1"
                     /translation="MAGIMQDSFGRVLDYVRISVTDRCNFRCIYCMPAEGIEWIPHEI
                     ILTYEDMLFLCSILTDLGVRKIRFTGGEPFVRKGFVPFLESVKEAHPHLRIAVTTNGS
                     LLEKWAERISGLGLDSLNVSLDTLNAEKFLQTTRTGDLQRVLRGIDAVRQTKGVPVKL
                     NSVVMRDFNFDEIAALVGFAAEKDATLRFIEFMPLDKDVWSQSQFVPAEEILSRLPDP
                     DQWMSCPSKENRSNICGPSRYFRNSVTGQRVGIISAVSDHFCHLCNRLRVTSTGVVRP
                     CLFGNFGVPLLEGLRAKDAGAVREGILHAVSRKPRRGATGVPSEEGAEAFQEERHMSQ
                     IGG"
     gene            complement(1419..>1877)
                     /locus_tag="P5219_07860"
     CDS             complement(1419..>1877)
                     /locus_tag="P5219_07860"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRX26712.1"
                     /translation="SLLARRPVFFRQQGIMVQKGNPRGIRTVSDLRREDVVIVNRQPG
                     AGTRVLLDFLFRKENISPTNVRGYENCSTTHFDAGNKVLRGFADAAAGIKSVADALGL
                     DFLPLAEEPYELVFFKSMEDHPGVAALLESLHDEGWRTMVDRMGGYRWPE"
BASE COUNT          424 a          535 c          474 g          444 t
ORIGIN      
        1 aatagtaagc aacccaacac tcacacccgt aagggcttcc atctccactc ccgtgacatc
       61 cctcgcggcg atggtgcagg aaattctgat cctgcccctt tcctccagga aataacattt
      121 cacttccgct ctatctatgc ggatgccatg gcacagtgga atgagttcgg gggttttttt
      181 cacggccata atgcctgcgg tctctgctat tttcaggaca tccccctttt ttgaatagcc
      241 gtcggccgag agctgttcgc acactgccct gtcaaggata acccatcctt cagcgacagc
      301 ggtgcgaagg gtttccggct ttccgctcac atctaccatc cggggccttc cttcgccgtc
      361 aagatgggaa aaaactggtt ccttatcctg cccgaaaaaa acgtcatctg ccatgggtta
      421 tcctccgatc tgggacatgt gccgttcttc ctgaaaggct tccgctcctt cttctgaagg
      481 aacgcccgtt gctccccttc tgggtttcct ggaaacggcg tgcaggatgc cttcccgtac
      541 agctccggca tccttggcgc gaaggccttc aaggagggga acaccgaaat ttccgaaaag
      601 gcagggccgc acgacgcctg tggatgtcac ccgaagccgg ttgcaaagat ggcagaaatg
      661 gtcggagacc gctgaaatga tgcctaccct ctgcccggtg acgctgtttc ggaaataacg
      721 ggagggaccg caaatgtttg acctgttttc ctttgacggg cagctcatcc actgatccgg
      781 gtccggaagc cgggaaagaa tctcctcagc ggggacaaac tgcgactggg accagacatc
      841 cttgtccagg ggcatgaatt cgataaacct gagagtcgcg tctttttcgg ctgcaaaccc
      901 gacaagggcc gctatctcgt cgaagttgaa gtctcgcatg acaaccgaat tcagtttgac
      961 aggaactcct tttgtctgcc ggacagcatc tattccgcga aggactcttt gcagatctcc
     1021 cgtgcgggtg gtctgaagaa atttttccgc gttcagggta tcaaggctta cgttcagcga
     1081 atccaggccc agtccggaga ttctttcagc ccatttttcg agaagggaac catttgtcgt
     1141 gactgcaatc ctgagatgcg gatgggcttc tttcaccgat tcaagaaaag gaacaaaccc
     1201 cttcctcacg aagggctctc cgccggtgaa cctgattttc ctgaccccga ggtcagtcag
     1261 aatgctgcac aggaagagca tatcttcgta ggttaaaatt atttcatgcg gtatccactc
     1321 aatgccctcc gcgggcatgc agtaaataca acgaaaattg cacctgtcag ttactgaaat
     1381 cctcacataa tcaagcaccc gtccgaaaga atcctgcatt attccggcca tctgtaacct
     1441 cccatgcgat ccaccatggt tctccatcct tcatcgtgga gcgattccag aagagcggcc
     1501 actccagggt ggtcctccat gctcttgaag aaaacgagtt cgtagggttc ttccgcaagt
     1561 ggaagaaaat caaggccgag agcgtcggcg acagatttta ttcccgccgc tgcgtcggca
     1621 aaccctctca aaactttgtt gcccgcgtcg aaatgggttg tgctgcagtt ttcgtagccc
     1681 cgaacgttcg tgggggagat attttccttc cggaaaagaa aatcgagaag gactctcgtg
     1741 cccgctcccg gctgacggtt cactatcacc acgtcttctc tccggaggtc agagacggtt
     1801 cggattcccc tgggattccc tttctgcacc atgatgccct gctgacggaa gaagacaggg
     1861 cggcgggcaa gaaggga
//
LOCUS       DAOTHD010000449         1875 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_73280_length_1875_cov_2.612088, whole genome shotgun sequence.
ACCESSION   DAOTHD010000449 DAOTHD010000000
VERSION     DAOTHD010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73280_length_1875_cov_2.612088"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="P5219_07865"
     CDS             complement(<1..595)
                     /locus_tag="P5219_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HRX26713.1"
                     /translation="MAKILIFGEEARRAMLRGIDKVADTVGVTLGPKGRNVVLEKKFG
                     SPTITNDGVTIAKEIELEDVYENTGAQLLKEVASKTNDIAGDGTTTATVFARAIIAEG
                     MKNVAAGANGMQMRSGIERAIDLVVDELKKKAISVKNRDKIAQVAAISANDKAVGQLI
                     AEAMDKVGKDGVITVEDSQTVGTTLEMVEGLQFDKGYI"
     gene            complement(624..914)
                     /gene="groES"
                     /locus_tag="P5219_07870"
     CDS             complement(624..914)
                     /gene="groES"
                     /locus_tag="P5219_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRX26714.1"
                     /translation="MNLKPLGDRIVVKVVNHEEKTRGGIVLPDTAKEKPTEGEVIAVG
                     TGKVLENGQKLPLEVKVGDRIIFSKYAGTEVKLDGDEYVIFSERDVLAIIDK"
     gene            complement(1114..>1875)
                     /gene="tsaD"
                     /locus_tag="P5219_07875"
     CDS             complement(1114..>1875)
                     /gene="tsaD"
                     /locus_tag="P5219_07875"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061711 - N(6)-L-threonylcarbamoyladenine
                     synthase activity [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRX26715.1"
                     /translation="AGPGLMGSLLVGVMAAKALSLAWNVPLIAVNHLEGHMFANVVSF
                     PELAPPFICMIVSGGHTEIVLAEDFGKYVFLGGTRDDAAGEAYDKVAKVLGLPYPGGP
                     VMDRLAASGNPGAFDFPVPMRGSSAGIVFSFSGLKTAAVTEIGRLRAQGKPVPVEDVC
                     ASFQRAVVDSLVGKLALAVKQTGVKNVALSGGVAANSALRAALKAQKGWNVFLPPVNR
                     CTDNAVMIAAAGCSAYLRGVRTSIDFAPDPSWELW"
BASE COUNT          364 a          548 c          545 g          418 t
ORIGIN      
        1 tgatgtaccc cttgtcgaac tgaaggcctt cgaccatctc aagggtggtg ccgacggtct
       61 ggctgtcctc gacggtgatg acgccgtcct tgccgacctt gtccatggcc tcggcgatga
      121 gctggccgac cgctttgtcg ttcgcggaaa tggcggcgac ctgggcgatc ttgtcgcggt
      181 tcttgacgga gatagccttc ttcttcagct cgtcgacgac aaggtcgatg gctctctcga
      241 tgccggaacg catctgcatg ccgttggcgc cggccgcgac gttcttcatg ccctcggcga
      301 tgatcgcgcg ggcaaatacg gtggcggtgg tggtgccgtc tccggcgatg tcgttggtct
      361 tggaggcgac ttccttcaga agctgggcgc cggtgttctc gtacacgtcc tcgagctcga
      421 tctccttggc gatggtcacg ccgtcattgg tgatggtggg agaaccgaac ttcttctcga
      481 gaaccacgtt ccggcccttg gggccgaggg tgacgccgac ggtgtcggca actttatcga
      541 taccgcgaag catcgcgcgg cgtgcttctt ctccgaaaat aagaatttta gccataatcg
      601 ttttacctcc tcaggaattc agactactta tcgataatcg caagcacgtc gcgctcgctg
      661 aaaataacgt actcgtcgcc gtcaagcttc acttcggtgc ccgcgtactt gctgaagatg
      721 atgcggtcgc cgaccttgac ttcaagcgga agcttctggc cgttctcaag gaccttgccg
      781 gtgccgacgg cgatgacttc gccctcggtg ggcttttcct tggcggtgtc gggaagaacg
      841 atgccgcccc tggtcttttc ctcatggttg acgaccttga ctacgatcct gtctccaagc
      901 ggtttcagat tcatggtaca aatccctcct aagaatgttt tacaaaatgc tagcactcct
      961 tcgagtcgag tgctaaatga ccggaataga tattacggat gaaggggggg aatctcaaca
     1021 cctgtagagt ggaaatatgg gagattatgg aggaatatct cgtttttttt cttatttact
     1081 tcgatttatc gagtttttca cttcaaagat gcttcaccag agctcccacg atgggtcggg
     1141 agcaaaatcg atggacgtcc ggaccccccg cagataggcg ctgcagcctg cggcggctat
     1201 catcacggca ttgtcggtgc agcggttcac gggaggcaga aagacgttcc atcctttttg
     1261 ggccttcagc gcagcccgca gggcgctgtt ggcggcaacc cctcccgaca gggccacgtt
     1321 ttttaccccg gtctgcttca ctgccagggc gagctttccc accagggaat ccaccacggc
     1381 cctctggaag gatgcgcaga catcctccac agggacgggt tttccttggg ccctcaggcg
     1441 gccgatttcc gtcaccgccg cagtcttgag gccgctgaag ctgaacacga ttcccgccga
     1501 acttccccgc atgggaacgg gaaaatcaaa agcccccgga ttgcctgaag cggcgagacg
     1561 gtccatgacg ggccccccgg gataaggcag gccgagaacc ttggcaacct tgtcgtaggc
     1621 ctcaccggcg gcgtcatccc gagttccccc caggaagaca tattttccga aatcctcggc
     1681 caggacaatc tccgtatgcc ctcctgagac gatcatgcag atgaacgggg gggcaagctc
     1741 cggaaaggaa acaacgttgg cgaacatgtg cccttcgagg tggttcacgg caatgagggg
     1801 cacgttccag gcaagggaga gggccttggc agccatgacc cccacgagga gggatcccat
     1861 gagccccggc ccggc
//
LOCUS       DAOTHD010000450         1873 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_73409_length_1873_cov_2.007701, whole genome shotgun sequence.
ACCESSION   DAOTHD010000450 DAOTHD010000000
VERSION     DAOTHD010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73409_length_1873_cov_2.007701"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1873
                     /locus_tag="P5219_07880"
     CDS             <1..>1873
                     /locus_tag="P5219_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRX26716.1"
                     /translation="SVLRDIDGLPAALLEISSLRSIFLQGKRTVLQFFLWLALFGGFQ
                     TAVVLLLLKWTVTGRLESARRLLEKIAFSGRVGERMPLSGSDEITSLSGSINSMLASL
                     ERLVENIPDPLFLSDGGRIVHANREACSLLGTCKSALIGEFVSSFLMEEDPLSQPQED
                     SSPVYEGKLLPPGRSGVPVEIHRQSFLLGERKLTLAVARDITRRRTLEDSLRRMAYFD
                     DKTGLPNRTRFLMLADEAVQSRESFTVALLDMDRFRLINDMIGPAKGDDLLREFSHRI
                     QGVLSPGDVVARVGGDAFGIILRKRETWEEASPVLIQVKKEVMTPLFVDGQTVFPSVS
                     MGVLPDAGRCGSASSALAKAETALLRAKGRGLGQTALFREDEAAGFPDILTAEGQLRK
                     ALEQGELTLHYQPIVRLKGKGVEGFEALVRWRHPDRGIIPPGEFIPLAEETGLITEID
                     RWAAGQVCRDINRFREMSGKGMKVSVNASARSILEEDYCARLAAETKGAGISPRLLTV
                     EITEGVLISNPSAASASLAALRREGISVALDDFGTGYSSLQYLSSLPIDRLKIDGSFI
                     RRLFTGDGDRRLVKGILALASDLGMETVTECIEREDEFLWTLEHGATHGQGYFFGYPR
                     "
BASE COUNT          352 a          581 c          591 g          349 t
ORIGIN      
        1 tttccgtcct ccgggacatt gacgggttac cggcagccct gcttgaaatc tcctccctgc
       61 ggagcatctt tctccaggga aaaaggacgg tgctccagtt ctttctctgg ttggccctct
      121 tcggcggatt ccagaccgcc gtcgtcctcc tcctcctgaa gtggaccgtc accggcaggc
      181 tggaaagcgc ccggcgcctc ctggagaaaa tagccttcag cggaagggtg ggagagcgga
      241 tgcccctgtc cggcagcgac gagatcacgt ccctctccgg atccatcaac tccatgctcg
      301 cctccctgga gcggctcgtg gaaaacattc ccgacccgct gttcctctcc gacggcggac
      361 ggatcgtcca cgccaacagg gaggcctgtt ccctgctcgg aacctgcaag tccgccctca
      421 tcggcgagtt tgtttcgtcc ttcctgatgg aagaggaccc cctttcccag ccccaggaag
      481 attcatcccc cgtatacgag gggaaactcc ttcctccggg acgaagcgga gtgcccgtgg
      541 aaatccatcg ccagtccttc cttctcggcg aacggaagct caccctcgcc gtggccaggg
      601 acatcacgag gcgcagaacg ctggaggact ccctccggag gatggcctat ttcgacgaca
      661 agacagggct tccgaaccgc acccggttcc ttatgctggc cgacgaggcc gtacagtccc
      721 gggaaagctt tacggtggcc cttctcgata tggacaggtt ccgcctcatc aacgacatga
      781 tcggcccggc gaagggggac gatctcctca gggagttcag ccatagaatt cagggcgttc
      841 tgtctcccgg cgacgtggtc gccagggtag ggggcgacgc cttcggcatc attctgcgga
      901 aacgggaaac ctgggaagaa gcctcgccgg tactgatcca ggtgaagaaa gaagtcatga
      961 ccccactttt cgttgacggg cagacggtct ttccaagcgt gagcatgggg gttctgcccg
     1021 acgcgggaag atgcggatcc gcatcttcgg ccctggcaaa ggcggagaca gcgcttcttc
     1081 gggccaaagg gcgggggctc gggcagacgg cccttttccg ggaggacgag gcggcgggat
     1141 tcccggacat cctcaccgct gaggggcagc tccgcaaggc tctcgagcag ggggagctga
     1201 cgctccatta tcagcccatc gtccgtctga agggaaaggg agtggagggg ttcgaggcgc
     1261 ttgtgcggtg gcggcacccc gaccggggga tcattcctcc cggcgagttt atccctctgg
     1321 cggaggagac aggactcata acggaaatcg accgctgggc cgccgggcag gtctgcaggg
     1381 atatcaaccg gttcagggaa atgtcgggaa agggaatgaa ggtttcggtc aacgcctccg
     1441 cccgttcgat cctggaagag gattactgtg cccgcctcgc cgctgaaacg aagggggcgg
     1501 gaatctcccc ccggctgctg acggttgaga tcaccgaggg tgttctcatc agcaatcctt
     1561 cggcggcatc ggcctccctg gcggcgctcc gcagggaggg catttcagtg gccctggacg
     1621 acttcgggac cgggtactcc tccctgcagt acctgagcag cctgcccatc gacaggctga
     1681 aaatcgacgg cagcttcatc aggaggctct ttaccggcga cggcgaccgc cgcctggtga
     1741 agggaatcct cgccctcgct tcagacctcg gaatggaaac ggtgacggag tgcatcgagc
     1801 gggaggatga atttctctgg accctggagc atggagccac ccacggccag ggatatttct
     1861 tcgggtatcc ccg
//
LOCUS       DAOTHD010000451         1871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_73489_length_1871_cov_3.513767, whole genome shotgun sequence.
ACCESSION   DAOTHD010000451 DAOTHD010000000
VERSION     DAOTHD010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73489_length_1871_cov_3.513767"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="P5219_07885"
     CDS             complement(<1..418)
                     /locus_tag="P5219_07885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HRX26717.1"
                     /translation="MNLSEARRRTAERLRASGLESPAREAELLLLDAAGISRSFLVAH
                     PFFSLEDAVVRKLSEMTARRCAGEPLQYILGSWEFYGRPLHVAPGVLIPRSDTEVLVE
                     QALSSLPHEGRFLDWGTGSGCIPLALLAERPDLSAVA"
     gene            complement(418..1494)
                     /gene="prfA"
                     /locus_tag="P5219_07890"
     CDS             complement(418..1494)
                     /gene="prfA"
                     /locus_tag="P5219_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRX26718.1"
                     /translation="MNYLGKLEAFEKDFETIEAKMADPSTHGNLKELQSLARRHSHLE
                     PVVAKYREYRKISADIEEARSLVYGSDQDMAELAREELAEKEPLLERLEQEIRILLLP
                     KDPNDEKSVIMEIRGGAGGEEAALFAADLFRMYTRFAEREGWKTEVLDYNETGIGGYK
                     EIIFRIDGNGVFSKLKFDSGVHRVQRVPATEAGGRVHTSTATVAVLPEAEDVDVEINT
                     EDLKIDTYRSSGAGGQYVNMTDSAVRITHVPTGIVVTCQDERSQLKNRVKAMAYLRAK
                     LYDAELRKQNEELASERRGQIGTGDRSERIRTYNFSQNRITDHRIGVTLYKLDQYLDG
                     DLYDLIEAMTVADQTERLHTIEAN"
     gene            complement(1491..>1871)
                     /locus_tag="P5219_07895"
     CDS             complement(1491..>1871)
                     /locus_tag="P5219_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HRX26719.1"
                     /translation="NAYESGAVMTPENIRNFSRIHPRCGTSFLLIVVAVSIVVFSLAG
                     SGSILWRIGSRIVLLPLVMGLSYEIIKGASCAGLAGRVLMAPALSFQYLTTREPDERQ
                     IEVALASLETALGRKLVMPAEESE"
BASE COUNT          348 a          567 c          545 g          411 t
ORIGIN      
        1 ctgccaccgc ggacaggtcc ggacgttccg ccaggagggc cagagggatg cacccggagc
       61 cggttcccca atccagaaac cgtccttcgt gcggtaggga ggaaagagcc tgttccacga
      121 gaacctccgt gtcggaccgg gggatgagga cccccggggc aacgtggaga gggcggccgt
      181 aaaactccca tgatccgagg atatactgca ggggttcccc ggcgcatcgc cgcgccgtca
      241 tctccgagag cttccggacg actgcgtctt ccagcgaaaa gaacgggtgg gcaacaagaa
      301 aggagcggga tattccggct gcgtccaaaa gaaggagttc cgcttccctc gccggggatt
      361 ccaggccgga ggcgcgcagt ctttccgctg tcctcctccg tgcttcggac aggttcatca
      421 gttcgcctct atggtatgca gcctttccgt ctggtccgca acggtcattg cctcgataag
      481 gtcataaaga tcgccgtcaa gatattggtc aagcttgtag agagtcacgc cgatcctgtg
      541 gtcggtgatt ctgttctggg aaaaattgta ggtccgtata cgttcggaac ggtcccccgt
      601 accgatctgg ccccgccgct ccgaagcgag ttcttcgttc tgtttccgga gttcggcgtc
      661 atacagcttc gccctgagat aagccatggc cttcaccctg tttttgagct gggagcgctc
      721 atcctgacag gttaccacta ttcccgtggg gacgtgggtt atccggacgg ctgagtctgt
      781 catgttcacg tactggccgc ccgcaccgct ggaccggtag gtgtcgatct ttagatcctc
      841 cgtattgatc tccacgtcaa cgtcctcagc ctcgggaaga acagccaccg tggcggtgga
      901 tgtatggacc ctccctccgg cctccgtggc gggaacccgc tggaccctgt ggactccgct
      961 gtcgaacttg agcttgctga acacgccgtt tccgtcgatc cggaatatga tttccttgta
     1021 cccgccgatc cccgtttcgt tgtaatcgag aacctcggtt ttccagcctt cccgctccgc
     1081 gaagcgggta tacatgcgga aaaggtccgc tgcgaaaagg gcagcttcct ctcctcccgc
     1141 ccctccgcgg atctccatga taacgctttt ctcatcgttg gggtccttcg ggagcaggag
     1201 gatccgtatc tcctgctcca gcctttcaag gagaggctct ttttcggcga gctcctctct
     1261 ggcgagttct gccatgtcct gatcggaccc gtagacgagg ctccgtgcct cctctatgtc
     1321 ggcggatatc ttccggtact cccggtactt cgccacaacc ggttcaaggt gcgaatggcg
     1381 cctggcaagg gactggagtt ccttcaggtt gccgtgggtc gacgggtcgg ccattttcgc
     1441 ttcgatggtc tcgaagtcct tttcaaaagc ctcgagtttt cccaggtagt tcattcagac
     1501 tcctccgcgg gcataaccag ctttcgcccg agggccgtct cgagggaggc aagggcaacc
     1561 tcgatctgcc gctcgtcagg ctccctcgtg gtgaggtact gaaaggacag cgcgggagcc
     1621 atgagaaccc tgcccgccag gccggcacat gacgccccct tgatgatctc gtaggaaagg
     1681 cccatgacca ggggcagcag gacaattctg cttccgatgc gccagagtat ggaacctgat
     1741 ccggcaaggg agaacaccac gatgctcaca gcaacaacga tgagaagaaa ggatgttccg
     1801 catcgcgggt ggatcctgga gaaattccgg atgttttccg gcgtcatcac cgctccggat
     1861 tcgtaggcat t
//
LOCUS       DAOTHD010000452         1866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_73772_length_1866_cov_2.817228, whole genome shotgun sequence.
ACCESSION   DAOTHD010000452 DAOTHD010000000
VERSION     DAOTHD010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73772_length_1866_cov_2.817228"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1866
                     /locus_tag="P5219_07900"
     CDS             <1..>1866
                     /locus_tag="P5219_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26720.1"
                     /translation="ERIGASIARLGAERSAKEEGKAVLEADPVLGCLSARQEVEGLEH
                     ESEQFRASLARRILLAKEIEDAEKAFRGNLENLCSWWTEDHLSGADVSAEAIEFARRT
                     AERKERLERRKGEEEKSLVQWNRLREDRRSEAASLDRETAGVEVRAKRATERWSLITD
                     LRAVFGELCDEEEDLANLEEARTTIISEKAVLAEEEPPEPGNMVALISGALLAVGGGG
                     TYQAWLTSDRLWFFGAVALFSASLLSFIAHRDQQKRYETALSWWTRRMDDLDLRMEET
                     LLLLESQKARVERLRSRREELGERLGIRAPRFANEMDELLEEGERDNSANERFAVLSE
                     RSRQMAAVLARMDSESEAMESALERTAGELDRLMDEWRRWLAGRQFDRNLAPKDMEAL
                     VPRILQLRSEKSVIESRKAELRELDSYVASVRQRILSLADLFSGAGVELPEEPDPSSI
                     RSLAVALRLAGERKGEIADLERDLAALSASLAELRENLEGNGGKTAELFTFAGSENEE
                     RYRDLAARWAERERLLEDASRERKVLLGLFGSGEEMEKAGGELLSLPAEDGRKERDRA
                     EEEAAVLEREIEAAADSRGRLALRLEQIAADERLGELLFSRTGMDRKLDEGVKEWL"
BASE COUNT          369 a          514 c          694 g          289 t
ORIGIN      
        1 gagcgaatcg gggcttccat tgcccgcctc ggggcggaac ggtcggcgaa ggaggaagga
       61 aaggcggtcc tggaggcgga tcccgttctc ggctgtctct ccgcccggca ggaagtggag
      121 gggctggagc acgagagcga gcagttccgg gcttctctcg ccaggaggat tctcctggcg
      181 aaggagatag aagacgcgga aaaggccttc aggggaaacc tggagaacct ctgttcctgg
      241 tggactgagg atcatctttc cggagccgat gtctcggcgg aggccatcga gttcgcacgg
      301 aggaccgcgg agcggaagga acgtctcgag aggcggaagg gcgaagagga gaagtctctt
      361 gtccagtgga accggctgcg ggaggaccgg agaagcgagg ccgcctccct cgaccgggaa
      421 acggcgggag ttgaggtcag ggcaaagcgg gcaaccgagc ggtggagcct catcaccgac
      481 ctgcgggccg tgttcgggga gctgtgcgac gaggaagaag atctggccaa cctcgaggag
      541 gccaggacca ccatcatcag cgaaaaagcg gttcttgccg aggaagagcc tcccgaaccc
      601 gggaatatgg tggccctcat ctccggggcg ctgctcgccg tcggcggggg aggcacctac
      661 caggcctggc tcacttcgga ccggttgtgg tttttcgggg cggtggccct tttcagcgcc
      721 tccctccttt ccttcatagc ccaccgggac cagcagaagc ggtacgagac ggccctctcc
      781 tggtggaccc gcagaatgga cgatctcgac ctgcggatgg aggagacact gctgctgctg
      841 gaaagccaga aggccagggt cgaaaggctc cggagccggc gggaggaact cggtgagcgg
      901 ctcggcatca gggctccgag attcgccaat gaaatggacg aactcctgga agagggggag
      961 agggataatt cggccaacga acggttcgcc gtgctgagcg agcggagcag gcagatggcc
     1021 gccgtgctgg cccggatgga cagtgagagc gaagccatgg agagcgccct tgagcggacc
     1081 gccggggagc ttgacaggct tatggatgaa tggcgccggt ggcttgccgg gcggcaattc
     1141 gaccggaacc tcgccccgaa ggacatggag gccctggtgc ccaggatact gcagctccgg
     1201 tcggaaaaat ccgtcatcga gtccaggaag gcagagctga gggagctcga cagctatgtc
     1261 gcctcggtga ggcagcgcat cctctccctt gcggatctct tctccggagc gggagtggag
     1321 cttccggaag agccggaccc ttcttccatc cggtctctcg ctgttgctct gcggctggcc
     1381 ggggagagaa agggggagat cgccgatctc gaacgggatc tggcggccct ttccgcctcc
     1441 ctcgcggaac tccgggaaaa cctggagggc aacggcggga aaacggcgga gctcttcacc
     1501 ttcgccggtt cggagaacga ggagcggtac agggaccttg ccgcccgctg ggccgaaaga
     1561 gagaggctcc ttgaggatgc ttcccgggag cggaaggttc tcctcggcct tttcggctcc
     1621 ggggaggaaa tggagaaggc gggaggggaa ctcctgtccc ttccggcgga agacgggagg
     1681 aaggaaaggg atcgtgcgga ggaggaagcc gccgtccttg aaagggagat cgaggccgcc
     1741 gctgactcga ggggaaggct tgccctcagg ctggagcaga tcgccgccga tgagcgcctc
     1801 ggtgagctgc tgttctccag gaccgggatg gaccggaagc tcgacgaggg agtgaaggag
     1861 tggctc
//
LOCUS       DAOTHD010000453         1865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_73839_length_1865_cov_2.294475, whole genome shotgun sequence.
ACCESSION   DAOTHD010000453 DAOTHD010000000
VERSION     DAOTHD010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73839_length_1865_cov_2.294475"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="P5219_07905"
     CDS             complement(<1..844)
                     /locus_tag="P5219_07905"
                     /inference="COORDINATES: protein motif:HMM:NF023990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3754 domain-containing protein"
                     /protein_id="HRX26721.1"
                     /translation="MGENSALEKFIPLRTADIVEALCGEQTLPGEDREKFRRVAGLVQ
                     AIYHYEYHRTLKQARDAYHPFNPDADTMKLCGPDEDYSARFDSMVEALEKLLEAANYK
                     ELSLDELNEIMTKAAPRGLNIEVNREKYEKILIYRRGRKSVPKAPDPSAWWNRLKKKN
                     GDPETEDMLQRLLILIKLKSEEDVEAFYDYYAETAGETGREKQRRLLEEAVVGKRESA
                     PSGKGSKIPTIFLKIFKNVPVSTLETLFPDVTIRMTLVDKGLILLPLIGGLFSVVNKV
                     IPALV"
     gene            complement(1042..>1865)
                     /locus_tag="P5219_07910"
     CDS             complement(1042..>1865)
                     /locus_tag="P5219_07910"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HRX26722.1"
                     /translation="KLGKPSAVRSVNLPEAGLRDHVVLAGGGTFAHRMAEILTGLGIA
                     HVIVEPVHDSFLKGKGAGLTLVLGNPAQDVILEAASPESARLSVVTAQDPLDAMDIIR
                     ALRRRTGEGTILALSVSDEHLPFLEENAVREIIRPDVEAGLEMARQVLLYLDFPATAV
                     QNELNAMRRALYGSLYTSLPEFEASTRLRDAAGLMSMEWVFLHEGTALAGKTLAESGI
                     RQTFGVSVVAVGRDGNVITGPDGSFRLEAGDYLGIVGDPERNARFIISQKPGKEK"
BASE COUNT          337 a          565 c          558 g          405 t
ORIGIN      
        1 ccaccagggc cggaatcacc ttgttcacca cgctgaagag tccgccgata agggggagca
       61 ggatgagccc cttgtccacg agagtcatgc ggatagtcac gtcgggaaaa agggtttcca
      121 gggtggagac ggggacgttc ttgaaaattt tcaggaagat ggtgggtatc ttcgaaccct
      181 ttccggacgg agcgctctcc cgttttccca ccacggcctc ctccagcagg cggcgctgtt
      241 tttcccgtcc cgtttcgccg gccgtttcgg cataatagtc gtaaaatgcc tccacatcct
      301 cctcgctttt cagcttgatc aggatgagaa gcctctggag catatcctcc gtctcggggt
      361 ctccgttctt ctttttgagc ctgttccacc acgccgacgg atccggggcc ttcgggacgc
      421 tctttctgcc cctgcggtag atgaggatct tttcgtactt ctcccggttg acctcgatgt
      481 tcagtccccg gggagcggct ttcgtcatga tctcgttcag ctcgtccagg gaaagctcct
      541 tgtagttcgc agcctcgagg agcttttcca gggcctccac catggagtcg aaccgggcgg
      601 agtaatcctc gtcggggccg cagagcttca tggtgtccgc gtccggattg aacggatgat
      661 aagcgtcccg ggcctgcttc agggtacggt gatactcgta gtggtaaatg gcctggacca
      721 gcccggcgac ccttcggaac ttctccctgt cctctccagg gagggtttgt tcgccgcaca
      781 gggcctccac gatatccgct gtgcgaagag gtatgaattt ttccaaagcg ctgttttccc
      841 ccatcctcag ccctccgttt caacagaagc ctttcccgtc gcggttccgt tcccgggaag
      901 gaaaaagact atgtccgccg gaagaaaaat gtatttttca aaaagcgcct ttagagtata
      961 tcacggggag aatcggaccc ggaacggaat tctgcccaac tgattccgcg gttgtgtgcc
     1021 ggggaggaca gcgaccctcc ttcatttttc ctttccgggc ttctgtgaga tgataaatct
     1081 ggcgttccgc tccggatcgc ccacgatgcc caggtagtcg cccgcctcca ggcggaatga
     1141 tccgtcgggg ccagtgatca cattgccgtc ccgtcccacg gccaccacgg acacgccgaa
     1201 agtctgcctg atgccggatt cggccagcgt tttcccggcc agggccgtgc cttcgtggag
     1261 aaagacccat tccatgctca tgaggccggc ggcgtcccga agcctggttg aagcctcgaa
     1321 ctccggcagt gaggtgtaga gggatccgta gagcgcccgg cgcatggcgt tcagttcgtt
     1381 ctggaccgcc gtcgcaggaa agtcgaggta caaaaggacc tggcgggcca tctccagccc
     1441 tgcctccacg tcgggccgga tgatttccct gacggcgttt tcttccagga agggaagatg
     1501 ctcatcgctc accgagaggg ccaggatggt accttccccg gtccgccggc ggagggcccg
     1561 gatgatgtcc atggcgtcca ggggatcctg ggcggtgacc acggagagcc gggcagattc
     1621 aggggatgcc gcctcgagga tgacatcctg ggcaggattg ccgaggacca gggtgagccc
     1681 ggcgcctttt cccttcagga agctgtcatg aaccggctcc acgatgacat gggcaattcc
     1741 caggcccgtc aggatttccg ccatccggtg ggcgaaggtt ccgcctccgg caaggaccac
     1801 gtgatccctg aggcctgctt cgggaagatt caccgagcgg acggcggagg gtttcccgag
     1861 cttca
//
LOCUS       DAOTHD010000454         1854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_74410_length_1854_cov_2.988883, whole genome shotgun sequence.
ACCESSION   DAOTHD010000454 DAOTHD010000000
VERSION     DAOTHD010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74410_length_1854_cov_2.988883"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="P5219_07915"
     CDS             complement(<1..445)
                     /locus_tag="P5219_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26723.1"
                     /translation="MKKAVYFFCDDPFDPVAGNVLAAVRHFFSPEETSLLIDGLPVLA
                     CSSPSGDLFSFVRTLKVVSHDYSRYLPVMTGHFADCDLAGIITWHEGENAPDGILTTH
                     TTGDVDSGAFGPADPECMRNLLLAMEQGRRRFLMHSRSAGPKAPES"
     gene            534..980
                     /locus_tag="P5219_07920"
     CDS             534..980
                     /locus_tag="P5219_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26724.1"
                     /translation="MRKKAAAWGAVVVFILLGALLRAHPSRESHFSGGGNRKTVLLFE
                     GEQVLSSDVSVRSGDGEYPEPASPDRDGSPLSRRTVPSGKRTGTGGRDLDSLKQGVKA
                     RKGNPGDMKEYLNEAERLRRERVRKSLEEIRRKVREYEPSIFDPTW"
     gene            complement(993..1475)
                     /gene="rimI"
                     /locus_tag="P5219_07925"
     CDS             complement(993..1475)
                     /gene="rimI"
                     /locus_tag="P5219_07925"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HRX26725.1"
                     /translation="MELDLCSSADLEGICSVEEQSSVSPWPREVIGADLSGDSPYLYL
                     CARAGDRILGFGTVLRKGKTSELANLAVLPEFRRRGVGSQLLAGLAEIAESLGSLRMN
                     LYVRESNTGAAKLYRLFGFSVLGRHTGYYADGEDALVMEASLPLRLPDDGDGGYPPPG
                     "
     gene            complement(1501..>1854)
                     /locus_tag="P5219_07930"
     CDS             complement(1501..>1854)
                     /locus_tag="P5219_07930"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HRX26726.1"
                     /translation="LDLVALRYAVRVNGMSSIALTKLDVLTDFERIPVCTGYEIDGKA
                     EEHFPSGVHRQWKAKPVYEYLEGWKEDLSGCAEFDRLPLQARKYVEYIETACGVPVNL
                     IGVGPGRDQTILRNF"
BASE COUNT          384 a          530 c          590 g          350 t
ORIGIN      
        1 tggactcggg ggccttcggt cccgccgatc tggagtgcat gaggaatctc ctcctgccct
       61 gctccatggc gaggaggaga ttcctcatgc actccgggtc ggcgggaccg aaggcccccg
      121 agtccacgtc cccggtggtg tgggtggtga ggatgccgtc cggggcgttt tcaccctcgt
      181 gccatgtgat gattccggcg agatcgcagt cggcgaagtg gcccgtcatc actgggaggt
      241 agcgggaata gtcgtggctg acaactttca gggtgcgaac aaaggaaaag aggtctcccg
      301 aaggagagct gcaggcaaga acgggaagcc cgtcaatgag gagagacgtc tcttccggag
      361 aaaagaagtg ccgcactgcg gccaggacat tccccgccac ggggtcgaag gggtcgtcgc
      421 agaaaaagta gaccgctttc ttcatgttct cttcctcctt tccggcctgc tcctgccgga
      481 agaatgccgg gaatggtttt gttctataat acacccggga gaaggaggac gacatgagaa
      541 agaaagctgc cgcatgggga gcagtagtgg tgttcatttt gctgggggcg cttctcaggg
      601 cccatccttc cagggaatct catttttccg ggggagggaa caggaagact gttcttcttt
      661 ttgaaggaga gcaggttctt tcatcggatg tttccgtgcg ttccggcgac ggagaatacc
      721 cggaaccggc ttcccctgac agggacggtt cccccctctc ccggcgcacc gtcccttccg
      781 gaaagcggac gggcacgggc ggcagggatc tcgattcgct gaaacaggga gtgaaagccc
      841 ggaaggggaa cccgggagac atgaaggaat acctgaacga ggcggagcgg cttcgaagag
      901 agcgggtgcg gaaatctctc gaagaaatcc ggcggaaggt cagggagtac gagccgagca
      961 tcttcgatcc cacgtggtga acagcccgaa gctcagcccg ggggcggata tcccccgtcg
     1021 ccgtcgtccg gaagacggag gggaagggaa gcttccatga ccagggcgtc ttctccatcg
     1081 gcgtagtagc ctgtatgccg cccgaggacg gaaaagccga agagacggta gagctttgcc
     1141 gcccctgtgt tggattctct cacgtagagg ttcatccgca ggcttcccag cgattcggcg
     1201 atttctgcaa gacctgccag gagctgggag cccacgcccc gtctccggaa ttccggaagc
     1261 accgcaaggt tcgcaagctc ggaagttttc cctttcctga ggacggtacc gaacccgagg
     1321 atacggtccc ccgccctggc gcacaggtaa aggtaggggc tgtccccgga aaggtcggcc
     1381 ccgatgacct cccggggcca gggagaaaca gacgactgct cctccaccga gcagattccc
     1441 tcaaggtcgg cggaggagca gagatcaagt tccacgggaa gaatgggaag attctccctt
     1501 ctagaaattc ctgagaatgg tctggtccct tccgggcccg accccgatga ggttgacggg
     1561 aacgccgcag gccgtctcga tatactcaac gtacttcctt gcctggagag gaagtctgtc
     1621 aaattccgca catcccgaga ggtcttcctt ccatccttcc aggtactcgt acaccggttt
     1681 cgccttccac tggcggtgaa ctccgctggg gaaatgctcc tccgccttcc cgtcgatctc
     1741 gtaccccgtg cagacgggaa tccgctcgaa gtccgtgagc acgtcgagct ttgtgagggc
     1801 gatggaggac atgccgttca ccctcacggc atagcgcagg gcgacaagat cgag
//
LOCUS       DAOTHD010000455         1852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_74517_length_1852_cov_3.330551, whole genome shotgun sequence.
ACCESSION   DAOTHD010000455 DAOTHD010000000
VERSION     DAOTHD010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74517_length_1852_cov_3.330551"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="P5219_07935"
     CDS             <1..783
                     /locus_tag="P5219_07935"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRX26727.1"
                     /translation="GGEPGEMFDRGVAALGGMGRFVKKGQTVVIKPNMSWDVPPERGG
                     NTNPELVERIVRRCLEAGASKVYAFDHTCDLWRNSYRNSGVEEAVKRGGGVAAPADTS
                     GYFQKVPLPGGRVLKETAVHELLLECDVFINVPVLKHHGGAGITMGMKNLMGTVWNRG
                     EFHSKGLHQCIADVSLFRRPALTVIDAYRMMTRHGPRGTSEGDVTVLKAQILSTDIVA
                     GDAAAARIFGADPKNVAYIRNAAEMGIGTMDLESLGVERIVL"
     gene            812..>1852
                     /locus_tag="P5219_07940"
     CDS             812..>1852
                     /locus_tag="P5219_07940"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRX26728.1"
                     /translation="MGKGRFLLALTVAALFLAWFLFRAGRADMPLLPALQAVPSILRW
                     WGGAAALALGAMVAGRAYCSVLCPLGTFQEAVWRAGRRRLSGYAPGSPVRYWILALVL
                     AAGAAGVAAVPALLDPYAAFGRGVTQLLRPSLAGGIDSLAGFFESLGLYGVISGTGRV
                     PFVPGLFWSTAAFFLALTLWSLRKGRPFCDALCPVGTFLGLFSRSSLLSIRFSGESCT
                     GCGLCGTVCPQECISVKERTVDADRCVLCLRCVDACPGGVLSLSFGGGGQSLSRRGLL
                     RTAAGTLAALGVAFWSSKKEDTVAAAASSRDEKLPVSPPGSKSRGNFTKKCVACTACV
                     AACPAGIIRPSLG"
BASE COUNT          269 a          583 c          650 g          350 t
ORIGIN      
        1 ggaggagagc cgggagagat gttcgaccgg ggggtggcgg ccctcggcgg aatggggcgg
       61 tttgtgaaaa aaggccagac cgtggtgatc aagccgaaca tgtcctggga cgttcccccc
      121 gaacggggag ggaacaccaa cccggagctg gtggagagga tcgtccgccg ctgcctcgag
      181 gccggcgcat cgaaagtgta cgccttcgac cacacctgcg acctgtggcg gaacagctac
      241 cggaacagcg gcgtggagga ggcggtgaag cgggggggcg gagtggccgc tccggcggac
      301 acgtccggat atttccagaa agtgcccctc cccggcggaa gggtgctgaa ggaaaccgcc
      361 gtccacgagc tgctcctgga gtgcgacgtg ttcatcaacg tccccgtgct gaagcatcac
      421 ggaggggcgg gtatcaccat gggcatgaag aatctcatgg gcacggtctg gaaccggggc
      481 gaattccact cgaaggggct gcaccagtgc atcgccgatg tcagcctgtt caggcggccg
      541 gccctgacgg tcatcgacgc ctaccggatg atgacccgcc acggcccgag ggggacgtcg
      601 gagggagatg tcaccgtgct gaaggcccag atcctgtcca cggacatcgt ggccggggat
      661 gcggcggcgg cgaggatctt cggcgccgat cccaagaacg tggcctacat ccggaatgcc
      721 gcggagatgg ggatagggac catggacctg gaatccctgg gggtggagcg gattgtcctc
      781 tgacaggccc cttccccgga agagaccgtg gatggggaag gggcgctttc tgcttgccct
      841 caccgtggcg gcgctgtttc tcgcctggtt tctgttccgg gcggggaggg cggacatgcc
      901 ccttcttccc gctcttcagg ccgttccgtc cattctccgg tggtgggggg gagctgcagc
      961 ccttgccctg ggcgccatgg tcgccgggag ggcctactgc tccgttctct gccccctggg
     1021 aaccttccag gaggcggtgt ggcgggcagg caggcggcgc ctttcgggat acgctccggg
     1081 atcgccggtg cggtactgga ttctcgctct cgtcctggcg gcgggcgctg ccggggtggc
     1141 ggccgttccg gcccttcttg acccctacgc cgccttcgga agaggtgtta cccagcttct
     1201 ccgcccctcc ctggccgggg ggatcgacag tctcgccggt tttttcgaga gtctcggact
     1261 gtacggggtc atctccggta ctgggagggt tcccttcgtt cccggacttt tctggagcac
     1321 cgccgcattc ttcctggccc tgaccctgtg gtccctccgg aaagggcgcc ccttctgcga
     1381 cgccctctgt ccggtgggga cattcctcgg gctcttttcc aggtcgtccc ttctctccat
     1441 ccggttttcc ggtgagagct gcaccggctg cggcctgtgc ggcaccgtct gcccccagga
     1501 atgcatctcc gtgaaggagc ggacggttga cgccgacagg tgcgttctct gtctccggtg
     1561 cgtggacgcc tgcccgggag gggtcctttc cctttccttc ggcggcggag gacagagcct
     1621 gtcccggagg ggtctgctcc ggacggcggc cggaactctc gccgctctcg gagttgcctt
     1681 ctggagctcg aagaaggagg ataccgtcgc cgcggccgct tcgtcccggg atgaaaagct
     1741 ccccgtctcc cctcccgggt cgaagagcag ggggaacttc acgaaaaaat gcgtggcctg
     1801 cacggcctgt gttgccgcat gtcctgctgg gattatccgc ccgtccctcg gg
//
LOCUS       DAOTHD010000456         1851 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_74564_length_1851_cov_4.320713, whole genome shotgun sequence.
ACCESSION   DAOTHD010000456 DAOTHD010000000
VERSION     DAOTHD010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74564_length_1851_cov_4.320713"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="P5219_07945"
     CDS             complement(<1..1227)
                     /locus_tag="P5219_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HRX26729.1"
                     /translation="MKKCTASSSSGPEATVPDPLTELLRRGARDLIAKAVEAELEALL
                     EDCSSLRLEDGRAAVVRNGYLPGRAVQTGIGDVEVRVPKVRDRSGSGIVFNSSLLPPY
                     LKRAKNVEEFLPWLYLKGVSTGGYGEALHSLLGENAKGLSANTISRLKEKWTEEHQEW
                     NRRDLSGKKYVYWWADGVYSRVRMDDKLCLLVIIGTTEKGEKELVAVEDGYRESEASW
                     HEVLSGLRSRGLKDGPKLAVGDGSLGFWNALRKEYPECVHQRCWVHKTANVLNKVPKS
                     LQGKMKADLHEIWMAETKEEAGKAFDRATAKYGAKYPKAIDCLTKDREELLAFYNFPA
                     VHWVHIRTSNPVESAFSTIRLRTAKTRNCGSRATTLAMVFKLAQSAEKRWRKLKGYEL
                     LGEVITGVIFKDGIRVS"
     gene            1447..>1851
                     /locus_tag="P5219_07950"
     CDS             1447..>1851
                     /locus_tag="P5219_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26730.1"
                     /translation="MKHVIGLDIGIASVGWSVINLERNRIEDLGVRVFSAPEHPKDGS
                     SLAKKRREAGGARKRIQRKRSRLLGIRQLFQEYGVLSLQEMDALFQSPLNKNPYEIRV
                     DGLSRHLSSTEWFQALYHIAKHRGFQSTKKTPP"
BASE COUNT          365 a          553 c          468 g          465 t
ORIGIN      
        1 cgatacacga attccgtcct tgaaaattac tcctgtgatg acttctccaa gaagctcata
       61 gcctttgagc tttctccacc tcttctctgc tgactgagcc agcttgaaga ccatcgccag
      121 cgtcgtcgcc ctcgatccgc agttgcgggt cttcgccgtc ctgagccgta tggtggaaaa
      181 ggccgattcc acgggattgc tcgtccgtat gtgcacccag tgcaccgcag ggaagttgta
      241 gaaagccagg agttcctccc ggtctttggt caggcagtca attgccttgg ggtatttggc
      301 cccgtatttg gccgttgccc tgtcgaaggc ttttcccgct tcttccttcg tctcggccat
      361 ccagatctcg tgaaggtccg ccttcatctt gccctggagc gatttcggca ctttgttcag
      421 cacgttcgcc gtcttgtgga cccaacaccg ttgatgaaca cattccggat attctttccg
      481 cagggcgttc cagaatccca gcgacccgtc cccgacggca agtttcggcc catccttcag
      541 tcccctggac cgcaggccgg agaggacttc gtgccagctc gcctcggatt cacggtagcc
      601 gtcttccact gccaccagtt ccttctctcc cttttccgtg gtcccgatta tgaccaggag
      661 acagagcttg tcatccatcc tcacccggct gtacactccg tccgcccacc agtagacgta
      721 ctttttgcct gagagatccc ggcggttcca ttcctggtgc tcttccgtcc atttctcttt
      781 gaggcgggag atggtattgg cggacaatcc tttcgcattt tcgcccagga ggctgtggag
      841 ggcctctccg tagcctccgg tggaaacgcc tttcaggtag agccagggca gaaattcttc
      901 cacattcttc gcccgcttca gataaggggg cagcagtgag ctgttgaaca caatgccgct
      961 gccgctccgg tcccggacct tgggtaccct cacttccacg tccccgattc ctgtctggac
     1021 agcccgcccg ggaagatatc cgttgcggac aaccgccgct cttccgtctt ccaggcgaag
     1081 agaggagcaa tcctcaagca gagcctccag ttcggcctct acagcttttg ctatgaggtc
     1141 tctggctccc ctccgcagaa gttcggtcag gggatccggg accgttgcct ctggaccgga
     1201 agagctggat gcagtacact ttttcatggc gtatccctcc aatgtcgatt gtgagcctgc
     1261 tagcccaatc ggcaggatac gccacttcat tttctatgtc catacaccag atctgagttt
     1321 aactcgcaag gttagccctg gttgtttaca accagggtat ccttttgtaa attttaccga
     1381 ctttcgagaa aatcacctca agtcgtagta ggtggcggcg tcgtacgaat gatcgggggg
     1441 ctcgcgatga aacatgttat cggtcttgat atcggaatcg cgtctgtcgg atggagtgtc
     1501 ataaatcttg aacgtaatag aatcgaggat ttgggcgtgc gggttttttc cgctcctgaa
     1561 catcccaaag acggatcctc tcttgcgaag aagcgtaggg aagccggagg cgctcgaaaa
     1621 cgaatccagc ggaagcgatc tcgccttctc gggatacggc agctcttcca agagtatggg
     1681 gttctctctt tgcaggagat ggacgccctc tttcaaagtc ccctcaataa aaatccttac
     1741 gaaatcagag tggacggcct ttcaaggcac ctttcctcaa cagagtggtt tcaagcgctc
     1801 taccacattg ccaaacatcg cggtttccaa tcgaccaaga aaacgccgcc a
//
LOCUS       DAOTHD010000457         1848 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_74745_length_1848_cov_3.710541, whole genome shotgun sequence.
ACCESSION   DAOTHD010000457 DAOTHD010000000
VERSION     DAOTHD010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74745_length_1848_cov_3.710541"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1558
                     /locus_tag="P5219_07955"
     CDS             <1..1558
                     /locus_tag="P5219_07955"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRX26731.1"
                     /translation="LLPLLQGMELPQSSPRILVLSPTRELAQQTAREAEWLSRYMDIS
                     VATLVGGLDMSTQIRSLKDGAVIVVGTPGRTLDHVNRGTLKTDGIHSIILDEGDHMLD
                     MGFRDELEGILDALPQRERTWLFSATMPREVRELSKKYLTDPVSISLVEDGEQHEDIV
                     HRAYLVPFRHKMEGLVNVLLWERPKRGLIFCHTRLETMSVAQRLSEEGFSAGSLHGEM
                     TQRERNAVLGSFKNGHLPLLVATNVAARGLDVEGVTHVIQIGLPDDRETFVHRSGRTG
                     RAGREGTDILLLSPQEAEKFKYMLGSAKVTVEWKNVPDLDAISRVQREIAEEKLLTVK
                     EAGEDEDYLLWAEDLLSRVSAKNLVARLLWTLSSKRSAGYNLSSELERELRRKSRIAG
                     EKDRAPGTRTLKARRPKGTMLRLSKGLADGWDVGRVLSAVCTSLNVDRSEVGAIRMKD
                     DHVLVELLPVALSRFEEDCRGLERCGLLEEGKGRDFLLPASPQGHQVRGRPSGPARPR
                     RQGKPEYRGR"
     gene            complement(1631..1831)
                     /locus_tag="P5219_07960"
     CDS             complement(1631..1831)
                     /locus_tag="P5219_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRX26732.1"
                     /translation="MSQGTVKWFNDSKGYGFITTDEGKDVFVHFSAIVGDGFKTLAEG
                     QKVSFDVVDGEKGPQAANVHKS"
BASE COUNT          424 a          536 c          528 g          360 t
ORIGIN      
        1 cctgctgcct ctcctgcagg gaatggaact tccccagtcc tcacccagaa ttctcgtcct
       61 ctcccccacc agggaacttg cccagcaaac agcccgggag gcggaatggc tttcacggta
      121 catggacata tccgttgcca cccttgtggg aggcctcgac atgtcaaccc agatccgctc
      181 gctcaaggac ggtgcggtca tcgtcgtggg cacccccgga aggaccctgg accacgtgaa
      241 caggggaact ctcaaaacgg acggcattca ttccataatt ctcgatgaag gagatcacat
      301 gctcgacatg gggtttcggg atgaactcga aggaattctc gacgcccttc cccagcggga
      361 gcggacatgg cttttttccg ccaccatgcc cagggaagtc agggaactct cgaaaaagta
      421 ccttactgac cctgtgtcca tctcccttgt ggaggacggg gaacagcacg aggatatcgt
      481 ccaccgggcg tacctcgttc ccttcaggca taaaatggaa ggactcgtga acgttcttct
      541 ctgggaacgg ccgaagcgtg gccttatctt ctgccataca cgcctggaaa ccatgtcggt
      601 ggcccagcgg ctttccgaag agggtttcag tgccggttct ctccacggag aaatgaccca
      661 gagagagcgg aacgccgttc ttgggtcctt caagaacggc catctgcccc ttctcgtggc
      721 gacgaacgtg gcagcccggg gcctcgacgt ggagggagta acgcacgtca ttcagatcgg
      781 actgcccgac gacagggaga ctttcgttca cagaagcgga cgtacaggcc gggccggacg
      841 ggaaggaacg gacatcctcc ttctttcccc ccaggaggcg gagaaattca agtatatgct
      901 cggttcggcg aaagtgaccg ttgaatggaa aaatgtccct gacctcgacg caatttccag
      961 ggttcagaga gagatcgccg aagaaaagct tctcacggtg aaagaagccg gagaagatga
     1021 agattacctc ctgtgggcgg aagaccttct gtccagggtc agcgcgaaga accttgtcgc
     1081 caggctgctc tggaccctgt cttcaaaacg ttccgccgga tacaatctct cctccgagct
     1141 ggaaagggaa ctccggagaa agagccgcat tgccggagaa aaggaccgtg cccccggaac
     1201 acggaccctg aaagcaagac gtcccaaggg tacaatgctt cgcctgagca agggcctggc
     1261 agacgggtgg gacgtgggca gggttctgag tgccgtgtgt acatccctga atgttgaccg
     1321 ttcggaggta ggcgccatcc ggatgaagga cgaccatgtc ctcgtggagc tgcttcccgt
     1381 ggccctctcc cggttcgagg aggattgccg cggcctggaa cgatgcggtc tcctggaaga
     1441 gggaaaagga agagactttc tccttcccgc gtctccccag ggccaccaag tgagaggaag
     1501 accttccggc ccggcccggc cccggaggca gggcaagccg gaatacaggg gcagataaca
     1561 taaaagaccg ccggttccga agaaccggcg gtcttttttc cctttggttt aaaaaatatc
     1621 gagggaaggg ttagctcttg tgaacgttcg cagcctgggg gcccttttcg ccgtcgacaa
     1681 cgtcgaaaga gaccttctgc ccttcagcga gggtcttgaa cccatcgcca acaatggcac
     1741 tgaaatgaac gaaaacgtcc ttgccctcgt cggttgtaat gaaaccgtac cctttgctat
     1801 cgttaaacca tttcacagtt ccctgactca tccaaagaca gcctccta
//
LOCUS       DAOTHD010000458         1838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_75257_length_1838_cov_2.943915, whole genome shotgun sequence.
ACCESSION   DAOTHD010000458 DAOTHD010000000
VERSION     DAOTHD010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75257_length_1838_cov_2.943915"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="P5219_07965"
     CDS             <1..210
                     /locus_tag="P5219_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26733.1"
                     /translation="EALKQATAEERKITYGMLSKSKEKVIADGGTVNSIDKAPFIAIA
                     VPIQDKLAEKLAIQDLVEKVRAAGK"
     gene            330..815
                     /locus_tag="P5219_07970"
     CDS             330..815
                     /locus_tag="P5219_07970"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRX26734.1"
                     /translation="MKKLIELLVRIQETLGMVLLSIFFLAILAQITARYLAIPLLWTE
                     EVANYAFIWAVFMGASVMVHYKAHFAFTFFSDRFRGRKGAAYDVFISAVLLCFTVPMT
                     LYGSTVVTEFWDYNWITLTWVKMGYTWLCLPITGGAMTLYLAGHIVEDIRILTSGEAV
                     S"
     gene            812..>1838
                     /locus_tag="P5219_07975"
     CDS             812..>1838
                     /locus_tag="P5219_07975"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRX26735.1"
                     /translation="MTPLLIVGLFVLLIFLGVPIAFVIGIVSLTGIATIPFIPNLTVF
                     MKMFNGLNSFVLLAIPLFIFAANLMNHGKITQKLVDFCIALVGNIRGGLAHANILVSM
                     IFAGVSGSSQADTAGVGKMLIPAMIETGYDKENAVGVTAASSTIGVIIPPSIPMVVYS
                     GLTNASVAALFLGGMIPGILVGLAMMLIVYLMGHRRNFPKYEKAAPGEVRKLFKESFP
                     ALLTPVIIVGGITTGWYTPTEAAAFASVYAMFISLFYYKTLKIRHLPGIIKETLKLSS
                     LSLFALATASALGELLGYYKASDHVAGFFAAHVGSPEMFMLIVIAFFLFVGTFMDAIP
                     AMILFMPV"
BASE COUNT          356 a          611 c          469 g          402 t
ORIGIN      
        1 gaggccctga agcaggccac ggccgaagag cggaagatca cctacggcat gctctccaag
       61 tccaaggaga aggtcattgc cgacggcggc accgtgaact ccatcgacaa ggctcccttc
      121 atcgccatcg ccgttcccat ccaggacaag ctcgccgaga agctggccat ccaggatctc
      181 gtggagaagg tccgcgccgc agggaaatag accgaaccgc tgaacagaag tctccatatc
      241 cgttccccgc gcccccggaa gggggcgcgg ggaatgttcc tttttcggga gaactgcgcc
      301 cggagagggt ttcacagagg aggagaaccg tgaaaaaact gatcgagctg ctggtccgca
      361 tccaggaaac cctgggaatg gtcctgcttt ccattttctt tcttgccatc ctggcccaga
      421 ttaccgcccg ctacctggcc attccgctgc tctggactga agaggtggcc aactacgcct
      481 tcatctgggc cgtttttatg ggagcttccg tcatggtcca ctacaaggcc catttcgcct
      541 tcaccttttt cagcgacagg ttccggggaa gaaaaggggc cgcctatgac gtcttcattt
      601 ccgccgttct gctctgcttc accgtcccca tgaccctcta cggaagcacg gtggtcaccg
      661 agttctggga ctacaactgg atcaccctca cctgggtgaa aatgggctat acctggctct
      721 gcctcccaat aaccggagga gccatgaccc tctacctggc ggggcacatc gtggaagaca
      781 tcagaattct cacctccggg gaggccgtat catgacgccc ctgcttatcg tcggcctttt
      841 cgtgctcctc atttttctcg gcgtccccat cgccttcgtc atcgggatcg tctccctgac
      901 gggcatcgcc accattccct tcatccccaa cctcaccgtg ttcatgaaga tgttcaacgg
      961 actcaactcc ttcgtcctgc tggccatccc tcttttcatc ttcgcggcaa acctcatgaa
     1021 ccacgggaaa atcacccaga aactggtgga tttctgcatc gcccttgtgg gaaatatccg
     1081 gggtggactt gcccatgcca atatcctcgt ctccatgatc ttcgcaggcg tctccggctc
     1141 gtcccaggct gacaccgccg gggtgggcaa aatgctcatc cccgccatga tcgagacggg
     1201 ctacgacaag gaaaacgccg tgggcgtcac cgccgcgtcc tccacaatag gggtcattat
     1261 ccctccgagc atcccgatgg tggtctactc cggcctgacc aacgcctccg tggcggccct
     1321 tttcctcggc ggcatgatcc ccggcatcct cgttggactg gccatgatgc tcatcgtgta
     1381 cctcatgggc cacaggcgca acttcccgaa gtatgaaaaa gcggcgcccg gagaggtgag
     1441 aaagctcttc aaagaatcct tccccgccct gctcactccc gtgatcatcg tgggcggcat
     1501 caccaccggc tggtacaccc ccactgaggc ggcggccttc gcttctgtct acgccatgtt
     1561 catcagtctt ttctactaca aaacgctgaa aatcaggcat ctgcccggga tcatcaagga
     1621 aacactgaag ctgagttccc tctccctctt cgccctggcc acggccagcg ccctcggcga
     1681 gcttttgggc tactacaagg cgtcggacca tgtggcgggc tttttcgccg cccacgtggg
     1741 aagccccgag atgttcatgc tcatcgtcat cgctttcttc ctgttcgtag gcaccttcat
     1801 ggacgccatc ccggccatga tcctcttcat gccggtca
//
LOCUS       DAOTHD010000459         1835 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_75435_length_1835_cov_2.730337, whole genome shotgun sequence.
ACCESSION   DAOTHD010000459 DAOTHD010000000
VERSION     DAOTHD010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75435_length_1835_cov_2.730337"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1378)
                     /locus_tag="P5219_07980"
     CDS             complement(89..1378)
                     /locus_tag="P5219_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRX26736.1"
                     /translation="MVLTLFLILLGGLALGVPIAVALGFATIFPGLTTPGFPASLAYV
                     VRNIVSALDSTPMLAIPLFILSGNIMTKGKISDKLFNFFAYFIGNFPAGIPMTAIVTC
                     LFYGAISGSGVATTAAVGGMAIPFLVSLGYDKVYSAALVATAGSLGVIIPPSIPFVTY
                     GVVTGVSIGSLFIAGIIPGLVIGASLMGYAFFYAKVHGEDRRLISEKVKALRSGGLLS
                     LFRESFWALLAPVIILGGIYSGIVTPTEAAAVSVFYALLVCLFVYRSLSFRELGAVLT
                     DTVRSYAPIVVLLSLAIVFGRVLALLQAPVALRDFIVSYFAGNRYIFLIALNAILLVL
                     GMFMDVGPAIAILAPMMLASATALDISPVHLGVIMVVNLAIGMATPPFGVDLFVAAPL
                     IKDQVMKVGMKAIPFIIAFIFALLLITFIPQMSLLFL"
     gene            complement(1381..>1835)
                     /locus_tag="P5219_07985"
     CDS             complement(1381..>1835)
                     /locus_tag="P5219_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRX26737.1"
                     /translation="EYVLSGLLVVIAVVMMLQVIMRYVFNASLSWAEEASRYAFVWSA
                     LVSIGYSIKENSILKVDTLVEALPAGLKHLLITLINLSVTLFFGYLLINSIPAVQRVI
                     MTGQTSPALKIPLGWIYFAAVAGFFLATVRSIQKTGQDLGFTIRKKGV"
BASE COUNT          492 a          454 c          542 g          347 t
ORIGIN      
        1 aactggatgt acgttgtcgc tgtccacttt cacctccctt gaggacgagg aacgtcactt
       61 cgtcccggat tttaaaagac tcccgcactt acaggaacag caggctcatc tgcggaataa
      121 aggtgatcaa aaggagtgcg aagataaagg ctatgataaa aggaatagct ttcatgccga
      181 ccttcattac ctggtccttt atgagcggcg ccgcaacgaa aagatcgact ccaaaaggag
      241 gtgtcgccat gccgatggca aggttgacca ccatgatgac gcccagatga acaggactta
      301 tgtcaagggc cgtggcggag gcgagcatca tgggggcgag aatggcgatg gcgggtccta
      361 cgtccatgaa cattccaagg acgaggagaa tggcgttcag agcgatgagg aaaatatacc
      421 tgttccctgc gaaatagctt acgataaagt cgcgcagggc cacgggcgcc tggagaagag
      481 ccagtacccg tccaaagaca attgccaggg acagcagcac gacgatggga gcgtatgatc
      541 tgacggtgtc ggtcaggacg gctcccagtt ctctgaatga caggctcctg tagacgaaaa
      601 ggcataccag cagggcgtaa aagactgata cggcggctgc ctcggtgggg gtcacaatcc
      661 cgctgtagat accccccaga atgattaccg gggcaaggag cgcccagaaa ctctccctga
      721 agagagacag caggccacca gagcggagag cctttacctt ttccgagatg agtcgtctgt
      781 cctccccgtg gactttcgcg tagaaaaagg cgtagcccat gagactcgcc ccgatgacga
      841 gtcccggtat gatccctgcg atgaagaggc ttccgatgga aactccggtg acaacaccgt
      901 aggtgacgaa gggaatgctg ggcgggatga tgaccccgag acttccggcg gtcgccacca
      961 gtgcggcgct gtaaaccttg tcatatccaa gggaaaccaa aaagggaatg gccatgccgc
     1021 ctacagcggc ggtcgttgct acgcccgagc ctgaaattgc tccgtaaaac aggcaggtga
     1081 cgattgcggt catgggaatc ccggcgggaa aattcccgat aaagtaagcg aagaaattga
     1141 acagtttgtc cgaaattttc ccctttgtca tgatgtttcc cgaaagtatg aaaagaggaa
     1201 tggcgagcat gggagtgctg tcaagagcgc ttacgatgtt tcggacgaca taggcaagag
     1261 aagcagggaa accgggggtc gtcaggcccg gaaagattgt ggcaaatccc agggcgacgg
     1321 cgattggtac accaagggca agaccgccga ggagaataag gaacaaagtc agaaccatgg
     1381 ttacactccc ttcttcctga tcgtgaatcc caggtcctgt cccgttttct ggatggaccg
     1441 gaccgttgcc aggaaaaagc cggccacggc cgcaaaatag atccagccca gggggatttt
     1501 cagggcggga cttgtctggc cggtcatgat gaccctttgg accgcgggaa tggagtttat
     1561 gaggaggtag ccaaaaaata aggtcacgga aaggttgatc agcgtgatca gaagatgctt
     1621 caatccggcg ggaagggctt caacaagagt gtcaactttc agaatactgt tttctttgat
     1681 ggagtagccg atgctcacaa gggcggacca cacgaaagcg tacctggaag cttcttcggc
     1741 ccacgacagg gatgcgttga atacgtatcg catgatgacc tgcagcatca tgacgacggc
     1801 aatgacaacg agcagaccgc tcaggacata ctctt
//
LOCUS       DAOTHD010000460         1832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_75614_length_1832_cov_3.755205, whole genome shotgun sequence.
ACCESSION   DAOTHD010000460 DAOTHD010000000
VERSION     DAOTHD010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75614_length_1832_cov_3.755205"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="P5219_07990"
     CDS             complement(<1..672)
                     /locus_tag="P5219_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HRX26738.1"
                     /translation="MSDTILENELNGQEAETVEQDNPAVSSEQETEAETTLKPAPKPR
                     YTFGQLAALLNPELRKIAKELGLSGFSSLRKDDLIIAILKAQAEELNYRFGGGTLEIL
                     QEGYGFLRPKGLLPSDHDVYVSASQIRRFGLRNGDVIWGLVRPPRDSEHYEALLRVEM
                     VNFSDPEAARRRPHFSAITPIFPDEKLSLETDPREISTRLVDLFAPIGKGQRALLVSP
                     PKAGKT"
     gene            complement(845..1279)
                     /locus_tag="P5219_07995"
     CDS             complement(845..1279)
                     /locus_tag="P5219_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26739.1"
                     /translation="MSRNGRFFLLLMSCVIILLLFFSFWAVRSNKAAGSFEIHNVLMC
                     DEVDDRMQPLGAGSVFSYGARQLCLWFDYSRASGGDGVRVRWYFRGRPIHSEVLRLTP
                     GDGAKAYCLLLEDGSPLPKGSYSVRISMYDRVFSDVPFVISE"
BASE COUNT          443 a          521 c          533 g          335 t
ORIGIN      
        1 ggtctttccc gccttggggg gcgacacgag caacgccctc tgtccctttc ctatgggagc
       61 gaaaaggtcc acgaggcggg tggaaatctc cctggggtct gtctccaggg aaagcttctc
      121 gtcaggaaaa atgggggtga tggcgctgaa gtgaggccgt cgtctcgctg cctcgggatc
      181 ggagaaattc accatctcca cccggagcag ggcctcataa tgttcggaat ccctcggcgg
      241 acggaccagg ccccagatga cgtcgccgtt ccggaggccg aaccggcgga tctgggaggc
      301 ggacacgtac acgtcatggt cgctgggaag cagtcccttc ggtctcagga aaccgtaccc
      361 ttcctgcagg atctcgaggg ttccgccgcc gaacctgtaa ttcagctcct cggcctgggc
      421 cttgaggatg gcgatgataa gatcgtcctt gcggagggag gaaaaacctg aaaggccgag
      481 ctctttcgct attttgcgaa gctcggggtt caggagggcg gcaagctgcc cgaaggtgta
      541 cctcggtttc ggggcgggct tgagcgtcgt ttccgcttcc gtctcctgtt cggaggaaac
      601 cgccggattg tcctgttcga ccgtctccgc ttcctgcccg ttcaattcgt tttcgagtat
      661 ggtgtcagac atgacggaaa ctaccggttc cccggccccg gaggactctg gaatactgcg
      721 cgggaaatgg aaagaatgga aaacacgaaa taaagcatcc ccttcctgaa acccccgtcc
      781 cggactccgg cacccagtgc ccggaaagac cggaacgcgg aaaaaatgaa aaaagcggca
      841 aagctcactc ggaaattaca aagggcacat ccgaaaaaac cctgtcatac attgaaatgc
      901 gaactgaata cgaacccttg ggaagagggg agccgtcttc gagtagaagg caatatgcct
      961 tggccccgtc gccgggcgtc agcctgagca cttcggaatg aatcggccgc ccccggaagt
     1021 accatcggac ccgaaccccg tctcctccgg aagcccggga ataatcgaac cagaggcaca
     1081 actgcctggc accgtagctg aacaccgatc ccgcgccgag gggctgcatc cggtcgtcca
     1141 cttcgtcaca catgagaacg ttgtgaatct cgaaggaccc cgcagccttg tttgaacgca
     1201 cagcccaaaa agagaaaaac agcagcaaaa taataacaca ggacatcaga agaagaaaaa
     1261 atcttccgtt cctcgacatt atacccaccg cccccgaaaa aacacaagtg caaagagaaa
     1321 aagaaggcgc ctttatccgg aacggccgcg aaaaccagtc cagaaaaacg aaaaaaccgc
     1381 gccgcctgga tcttccccga agcactctac cacgaatatc atgtagaagg caagaaggag
     1441 cggattcctg tcggcctgac gagcgttttt tgtcattccg aggagcgaag cgacgaggaa
     1501 tctcggtttt gcccttgctt gtcatcctga ggagcgcagc gacgaaggat ctcgggtttg
     1561 aatggggcca tgatcaaaag cgagatcctt cgggcggtga agcacaccct caggatgaca
     1621 gcgtggcggt tcgcgcatcc ccgaagggga gggatctcgg tcttgccctt gcctgtcatc
     1681 ctgagcgaac gcgagggacc tcggtcttgc ctttgctgtc atcctgagga gcgcagcgac
     1741 gaaggatctc ggggttggat ggggccataa tcaaaaacga gatccttcgg gcggtgaagc
     1801 acaccctcag gatgacagcg tggcggttcg cg
//
LOCUS       DAOTHD010000461         1832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_75636_length_1832_cov_2.355656, whole genome shotgun sequence.
ACCESSION   DAOTHD010000461 DAOTHD010000000
VERSION     DAOTHD010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75636_length_1832_cov_2.355656"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /gene="nrdD"
                     /locus_tag="P5219_08000"
     CDS             <1..967
                     /gene="nrdD"
                     /locus_tag="P5219_08000"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HRX26740.1"
                     /translation="ELDDQDLLDARTPWKLRFCALQNVTLNLPRLAYRSAGDRETLFD
                     LVDHYMEVAAEAHVQKRKFLGGILAQGKKGPLSVLCMDHDGEAYLRLEKAKYLIGILG
                     VNEMVQVMTGSQLHESKEALDLALAVVQYMNLKCEMLSERHGLTMVLEQTPAESTAYR
                     FAKLDLKSWPEAASVVKGDPGTGEVYYTNSSHIPYSVRMDPADKVLREGSFHPMISAG
                     AITHLWMGEHKPDPDALASFIRKVFLHSENAQVAFSPEFTICNGCGRVSRGLRTECAW
                     CGSGDVDGITRITGYFTRTSSCNGGKRAELRDRVRSSLQPSGLRE"
     gene            1071..>1832
                     /locus_tag="P5219_08005"
     CDS             1071..>1832
                     /locus_tag="P5219_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRX26741.1"
                     /translation="MKLRNVLLAAILVLVLAGTAVAADTIKIGVYLPLTGRTAFGGQL
                     ELEGVQMAHKELPELLGKKVELVIVDNKTDKVEAANAVKRLVERDKVVAVIGTYGSSL
                     AMAGGEVAEAAKIPMMGTSCTNPLVTQGKKYVFRACFIDPFQGAGAATYAVKTLNMKK
                     AALLVDVANDYCVGLGNFFKQSYKKLGGEIVAELMYNSGDQDFTAQLTEIISKKPDLL
                     YIPADFAEGAIIMKQARELGAEFQIMGGDAMDNPEI"
BASE COUNT          382 a          566 c          556 g          328 t
ORIGIN      
        1 tgaactggac gaccaggatc tcctcgatgc ccgcacgccg tggaagcttc gcttctgcgc
       61 cctgcagaac gtgaccctga acctgccccg tctcgcctac cggagcgccg gcgaccggga
      121 gaccctgttc gacctcgtcg accattacat ggaggtcgcg gcggaggccc acgtccagaa
      181 aaggaagttc ctcgggggca tcctggccca gggcaagaag gggcccctct ccgtgctctg
      241 catggaccac gacggtgagg cgtatctccg gctggagaag gccaagtatc tcatcggcat
      301 cctcggcgtg aacgagatgg tccaggtgat gaccggatcc cagctccacg agtcgaagga
      361 ggcgctggac ctcgccctcg cggtggtcca gtacatgaac ctgaagtgtg aaatgctctc
      421 cgagcggcat ggtctcacca tggtgctgga gcagactccc gccgagagca ccgcctaccg
      481 gttcgccaag ctcgacctca agtcctggcc cgaagctgcc tcggtggtga agggcgatcc
      541 cggcaccggt gaagtctatt acaccaacag cagccacatt ccctattccg tccgcatgga
      601 cccggcggac aaggtcctcc gggaaggaag cttccatccc atgatttcgg cgggggccat
      661 cacccacctg tggatggggg agcacaagcc ggatcccgac gcgctcgcgt ccttcatacg
      721 gaaagtgttc ctccactcgg agaacgccca ggtggctttc agtcccgagt tcaccatatg
      781 caacggctgc ggccgggtga gccgggggct ccggacggag tgcgcctggt gcgggagcgg
      841 cgatgtggac ggcatcacga ggatcaccgg ctatttcacc aggacctcga gctgcaacgg
      901 cggcaagagg gcggaactcc gggaccgggt gaggtcatcc ctgcagccgt ccggtctgcg
      961 ggaatgattt ccataattcc tgaaaggaca aaaactcttc ctgtatgcgt cttttttgtg
     1021 tataatttcg gggaagtctg taaatcatat ccgaggaggg aaaaaagcaa atgaagcttc
     1081 gtaatgtact gcttgcggca attcttgttc tggttcttgc gggaactgcg gtggcggccg
     1141 ataccatcaa gatcggcgtg tatctccccc tgacgggcag gacggcgttc ggcggccagc
     1201 ttgagctcga gggcgtccag atggcccaca aggaactgcc cgaacttctc gggaagaaag
     1261 tggagcttgt catcgtcgac aacaaaaccg acaaggttga agcggcgaat gccgtgaaga
     1321 ggcttgtgga gcgcgacaag gtcgtcgccg tcatcgggac ctacggctcc tccctcgcca
     1381 tggcgggcgg cgaagtggcc gaagcggcaa agattcccat gatgggaacc tcctgcacca
     1441 accccctcgt gacccagggc aagaagtacg tcttcagggc ctgcttcatc gatcccttcc
     1501 agggtgccgg cgccgccacc tatgctgtga agaccctcaa catgaagaag gccgccctgc
     1561 tcgtggacgt ggccaacgac tactgcgtgg gcctcggcaa cttcttcaag cagtcctaca
     1621 agaaactcgg cggcgagatc gtcgccgaac tgatgtacaa ctcgggcgac caggacttca
     1681 ccgcccagct cacggaaatc atcagcaaga agcctgacct tctctacatt cccgccgact
     1741 tcgccgaagg ggccatcatc atgaagcagg cccgggagct cggcgccgag ttccagatca
     1801 tgggcggcga cgccatggac aaccccgaga tc
//
LOCUS       DAOTHD010000462         1826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_75992_length_1826_cov_2.835686, whole genome shotgun sequence.
ACCESSION   DAOTHD010000462 DAOTHD010000000
VERSION     DAOTHD010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75992_length_1826_cov_2.835686"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="P5219_08010"
     CDS             <1..1140
                     /locus_tag="P5219_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRX26742.1"
                     /translation="ILARDTGIPLFVPADNADEVSLVEGCEAWGAESLGDVIAFLRGE
                     KKLIPVVPAVPEAKPPIADPDFADIRGQSGAKRALEIAAAGHHNILFTGAPGSGKTLL
                     ARALRGILPPLSRDEFLEAALVRSTLGIPFDRGMHPPFRTVHHTASIVSICGGGATLR
                     PGEVSLAHRGVLFLDEFPEFRRDLLEALRQPLEDGRITVSRASGSVVYPCRVLLVAAC
                     NPCPCGWDGDPVERCTCSATERERYRRKISGPILDRIDLHLAVPRLLPEELVAVESEG
                     GEPSAAIAARVRKAREIQRERWAGTGFTCNAELPERLVKRSLSLSPDVRPFLASMAER
                     LRLSGRGISRVLKVSRTIADLAGSRDVEVPHVAEALAYRDGGEKR"
     gene            1137..>1826
                     /locus_tag="P5219_08015"
     CDS             1137..>1826
                     /locus_tag="P5219_08015"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRX26743.1"
                     /translation="MTGLMRVLLLLAASGGFPPALWDSFSKEDLPPEAFLDEGPGLWE
                     RLGYSETVRNNLARLAATGWPEREEERAARAGVRIVPFDSRDYPRSLAGTPSAPLLLY
                     VRGKWPVAGPTVAVVGTRKCSSYGWRTAAEIGRAVAGAGGVVISGGAAGIDGAAHEGC
                     LDGGGATTAVLGTGVDVVYPRGHEGLFGRIVREGGALVAEYPLGAPPRQWRFPERNRI
                     IAGMADRLVVVE"
BASE COUNT          296 a          584 c          642 g          304 t
ORIGIN      
        1 attctcgcca gggatacggg aattcccctc ttcgtgccgg cggacaacgc tgacgaagtc
       61 tccctggtgg aggggtgcga agcctggggg gccgaaagcc tcggcgacgt gattgccttt
      121 cttcggggag aaaaaaaact gatccccgtg gtccctgcgg tccccgaggc gaagccgccc
      181 attgccgatc cggatttcgc cgacatacgg ggccagtcgg gggccaagcg ggccctggaa
      241 atagccgccg cggggcatca caacatcctg ttcaccggcg cccccggcag cggcaaaact
      301 cttctggccc gtgccctccg tggaattctc ccgcccctgt cccgggacga gtttctcgag
      361 gcggcccttg tccggagcac cctgggcatt cccttcgacc ggggaatgca ccctcccttc
      421 agaacggtcc accatacggc cagcatcgtc tccatctgcg ggggcggcgc caccctccgg
      481 cccggcgagg tcagcctggc ccaccggggg gttctcttcc tcgacgaatt tcccgaattc
      541 cgccgggatc tcctggaagc cctcaggcag cccctggagg acggtcggat caccgtgagc
      601 cgggcatcgg gctcggtggt ctatccctgc agagtgcttc tcgttgccgc gtgcaaccca
      661 tgcccgtgcg ggtgggacgg tgaccccgtg gaacggtgta cctgctcggc gacggagcgg
      721 gaacggtaca ggcgaaagat ttccggcccc atcctcgaca ggatcgacct ccacctggca
      781 gtcccgaggc tgcttccgga agagctcgtg gcggtggaat ccgagggcgg cgaaccgagt
      841 gccgccattg ccgcgagagt gaggaaggcc cgggagatcc agagggagcg ctgggccgga
      901 acagggttca cctgcaacgc cgagctgccg gaacggctcg tgaagcggtc cctctccctt
      961 tcaccggacg tgaggccctt tctcgcctcc atggcggagc gcctccggct ctcaggcagg
     1021 ggcatcagca gggtgctcaa ggtctcccgg accatagcgg acctggcggg aagcagggac
     1081 gttgaggtgc cccatgtggc ggaagccctg gcctaccggg acggagggga aaaacggtga
     1141 caggtcttat gagagtgctc ctgctccttg ccgcgtcggg aggatttcct cccgccctct
     1201 gggactcttt cagcaaggaa gatctccccc ccgaggcgtt tcttgatgaa ggccccggac
     1261 tgtgggaacg gctgggatat tcggagacgg tccggaacaa tcttgcgcgg cttgctgcga
     1321 cagggtggcc cgagcgggag gaagagcgtg ccgcccgggc gggagtgcgc atcgtcccct
     1381 ttgactccag ggactatccc cggagcctgg ctggaacgcc ctccgccccc cttcttctgt
     1441 acgtcagggg gaaatggccc gttgccgggc cgaccgttgc cgtggtggga acaagaaaat
     1501 gcagttccta cggatggcgg acggcggcgg aaatcggccg ggcggtggcc ggggcgggag
     1561 gagtggtgat cagcggcgga gccgccggta tcgacggagc cgcccacgaa gggtgcctcg
     1621 acggaggagg ggcgaccaca gcggttctcg ggacgggagt ggacgtggtc tatccccggg
     1681 ggcacgaagg tctcttcggg cgcatcgtcc gggagggagg cgccctggtc gccgaatatc
     1741 ccctcggcgc tcccccgagg cagtggcgtt tccccgagcg gaaccggatc atcgcgggga
     1801 tggccgatcg tctcgtggtg gtggag
//
LOCUS       DAOTHD010000463         1821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_76289_length_1821_cov_3.387316, whole genome shotgun sequence.
ACCESSION   DAOTHD010000463 DAOTHD010000000
VERSION     DAOTHD010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76289_length_1821_cov_3.387316"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="P5219_08020"
     CDS             <1..308
                     /locus_tag="P5219_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26744.1"
                     /translation="MVPMFRCGLDLSNFSFAICSNLPVNVVRPQPGHYRLFESIRHQR
                     EDAPDHGAYCSIFTEKTRAEARKRNHVSSPLSKKKILPAPFLPVEPFPVNFLQIAES"
     gene            477..>1821
                     /locus_tag="P5219_08025"
     CDS             477..>1821
                     /locus_tag="P5219_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26745.1"
                     /translation="MKPSIRKTVPLMAVLVFSCFSAVAGADFAGDLLVRAARYAALEY
                     LGAELSVDGIEGNPLKGFSSGRITLSSKGDLLLSADSLHLKINPRSLLSMKPVISSFS
                     ILSADIDGEKLSRHIASLDPLSGDRSIAIQRADMVNSTVTFGPNRANIGLLGVSFSEN
                     AASADIDISVNTVPIRGRAELDLSGGGLAVRSLILRAGKGEIEALGTIVPALSVGGFI
                     TGLDMTEIAALWPGVPPGTFRGILSLSFAGEGVWNAPLFSGDLHLESGLLRGIPLDRA
                     DARWSFSENRLFFSPVDAMAASVPITGRFSLRFSPGKIPAAEGSFSAEGIDLQSVSRL
                     LPALKDVGGRIETFTGRLSGPVDSLSGTGELRSREFSLFGVAASNAELSAEFDPDSVK
                     LAGKAGILEGTASLSGIVEKYTSAPRLNLTASARSFNLRKAFSLARGFRIPVLSGT"
BASE COUNT          414 a          533 c          440 g          434 t
ORIGIN      
        1 cgatggttcc gatgttcaga tgcggcttgg acctctcaaa tttctccttc gccatatgta
       61 gcaacctccc tgtaaatgta gtccgccctc agccagggca ttataggctt tttgaaagca
      121 tccgccatca gcgggaggat gctcccgacc atggtgctta ttgtagcata tttaccgaaa
      181 aaacaagggc agaagcaaga aaacggaatc acgtttcgag ccctctttca aagaagaaaa
      241 ttctaccagc accctttctc cctgtcgagc cttttcctgt caatttcctg caaattgccg
      301 aatcctaaga cagaacaggg ataaggatca gtccctccgg acggaccttc gggatattat
      361 aatgctataa ttatttccgg atattttgaa cattacacat tttcaccgca ttcctgacag
      421 cacatcagat gtaagccagg ttctccgcca acggaactga aaggaggcat tgaaccatga
      481 aacccagcat tcgaaaaacg gtccccctca tggctgtcct tgttttttca tgcttttccg
      541 ccgtcgccgg ggctgatttt gcaggagacc tgctggtccg cgcagcacgg tacgccgcac
      601 ttgagtattt aggcgccgaa ctttcggttg acggcataga agggaacccg ttgaagggtt
      661 tttccagcgg cagaataacc ctctcctcaa aaggagacct gcttctttcc gcagactccc
      721 tccacctcaa gatcaacccc aggtcccttc tctcaatgaa acccgtcata agttcctttt
      781 ccattctcag cgccgacatt gacggtgaaa aactctcccg tcacattgcc tcactggacc
      841 ccctttccgg ggaccgttca atagcaatcc agagagcgga catggtcaac agtaccgtca
      901 cgttcggccc caacagggca aacattggac tcctcggcgt ttcattctcg gaaaatgcag
      961 catctgcgga cattgacata tcggtcaata ccgtcccaat aaggggaaga gcagaacttg
     1021 atctctcggg aggaggtctt gcggtcagat cgctcattct gagggcaggc aaaggagaaa
     1081 tagaggcact tggcaccata gtccccgccc tctcagtcgg cggattcatc accggcctcg
     1141 acatgacgga aattgctgcc ctctggcccg gggttcctcc cggaacattc aggggaatcc
     1201 tgtctctttc cttcgccgga gaaggggtat ggaacgctcc cctgttctcc ggagaccttc
     1261 accttgagag cggattgctg agaggcattc ctctcgacag ggcagatgcc cgatggagtt
     1321 tttcggaaaa caggcttttc ttttctcccg tcgatgccat ggctgcctcc gtccccatca
     1381 cgggccgctt ctccctccgc ttctctcccg gaaagattcc tgccgccgaa ggttctttct
     1441 ccgccgaggg aattgatctg cagtcggtca gccgcctgct tccagcctta aaagacgtgg
     1501 gcggacgaat agagaccttc accgggagat tgtcaggacc tgtggactct ctcagcggta
     1561 caggagaatt gcggtcccgg gaattcagcc tcttcggcgt tgctgcgtca aatgcggagc
     1621 tttccgcaga attcgacccc gactcggtaa aactggccgg aaaggcagga atcctggaag
     1681 gaacggcctc cctttcaggt atcgtcgaaa aatacacttc ggctccaagg ctgaatctca
     1741 cagcatcggc ccgttccttc aatctccgga aggcgttttc cctcgcccga ggtttcagga
     1801 ttcccgtact ttcaggtaca g
//
LOCUS       DAOTHD010000464         1820 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_76349_length_1820_cov_3.139377, whole genome shotgun sequence.
ACCESSION   DAOTHD010000464 DAOTHD010000000
VERSION     DAOTHD010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76349_length_1820_cov_3.139377"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="P5219_08030"
     CDS             <1..238
                     /locus_tag="P5219_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRX26746.1"
                     /translation="YREISSCSNCEDFQARRMNTRFRPADGGKPRFVHTLNGSGIAIG
                     RALIAVLENFQREDGSIGIPEVLVPYMGGVTEIR"
     gene            395..>1820
                     /locus_tag="P5219_08035"
     CDS             395..>1820
                     /locus_tag="P5219_08035"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HRX26747.1"
                     /translation="MAEVLFFVPAAVVLCFLLQRRCRRSMEMPQYNYFRDLLLVAAWV
                     LAALWAGSSVSRFIVGMAAFASLIGTCQHIRPHWGWKTVYLFVGLLFAFFGPKIGFIG
                     LPDGQYHYFSPEVSIIATALWVGVFPVLLQQLDNIPGMAGHLLAVSFSLLLLATVFSG
                     QDLPDAFFMSLCGLAFLGVFWSRHGHMYRRMGESLSAFWGVLVAGTSVLGVSKGITFS
                     TLLLLPLGLFAIPIMEASLHFAGLAFSSKPHGAAVLYRKLISRGLDHPSAVRFITSLC
                     ALGGAVIAISQLGNSLFMMLWVSATILFTGLAIMPFIRKMKENAEMPLKPSIWSTSVD
                     NVSMDYALAKTRAAALSGKGVSLVSTVNALAVMEAEKNRGYADALRSSFLTLADGAGL
                     VWALRFLGTPVQERVAGIDFMMRLSRTAAAERLPVYLLGAKEEAVKGAAETLKEQYPD
                     LVIAGWRNGYFDPCDASVAGTVRDS"
BASE COUNT          308 a          551 c          531 g          430 t
ORIGIN      
        1 gtacagggag attagctcct gcagcaactg cgaggatttc caggccagga ggatgaacac
       61 caggttccgt cccgccgacg gcggcaagcc ccggtttgtc cataccctga acggttcagg
      121 catcgccatc ggcagggccc tgatcgccgt tctcgagaac ttccagaggg aagacggctc
      181 catcgggata ccggaagtcc ttgttcccta catgggaggc gtcacggaaa tccgctgata
      241 ttgggccatt atactcgcgg cgttgtcctc tccgccaggg aagtctgggg attcatacag
      301 catccggggt ggaaaaatag ggaagctgct ggtacaatcg aaggtggagc cttatatcca
      361 gggagggcta atcatactgt ttgctgagag attcatggcc gaggttttat tttttgttcc
      421 ggcggcggtg gtgctgtgct ttcttttgca gcgccgctgc agaagaagca tggagatgcc
      481 ccagtacaac tatttccgtg atcttttgct cgtggcggcg tgggttctcg cggccctctg
      541 ggcggggagt tccgtatccc gcttcatcgt ggggatggcg gccttcgctt ccctcatagg
      601 gacgtgccag cacatccggc cgcactgggg gtggaagacg gtttaccttt tcgtcggcct
      661 tctgtttgca ttcttcgggc cgaagatagg ttttatcggc cttcctgacg ggcagtatca
      721 ctatttttca cctgaagttt ccatcattgc gaccgccctc tgggtgggtg tttttcccgt
      781 ccttctccag cagttggaca acattcccgg catggcgggg catcttctcg ccgtcagctt
      841 ttcgctgctc ctcctcgcca cggtattttc ggggcaggat cttcccgacg ccttcttcat
      901 gtctctctgc ggtcttgcct tcctcggcgt gttctggagc cgccacggac acatgtaccg
      961 ccggatgggg gaatcccttt cggctttctg gggcgtcctc gtggccggca catcagtgct
     1021 gggcgtgagc aagggaatca ccttcagcac tcttctgctc cttccccttg ggctgtttgc
     1081 catccccatc atggaggcgt ccctccactt tgccggcctt gccttttctt ccaaacccca
     1141 cggggcggca gtcctgtaca gaaagctcat cagccgcggg cttgaccacc cgagcgccgt
     1201 ccgattcatc acgtccctct gcgccctcgg cggagcggtc atcgccatct cccagctcgg
     1261 gaattccctc ttcatgatgc tgtgggtcag cgccaccatc ctcttcaccg gccttgccat
     1321 catgcctttc atccgaaaaa tgaaggagaa tgcggaaatg cccctgaaac cctccatctg
     1381 gagcacctct gtcgataatg tctccatgga ctatgctctc gccaaaacga gggcggctgc
     1441 actgtccggc aaaggggttt ccctggtgtc cacggtgaac gcccttgccg ttatggaagc
     1501 ggaaaagaac cggggctatg ccgatgcgct tcgcagctcc ttcctcaccc ttgccgacgg
     1561 cgccggcctt gtctgggccc ttcgttttct cggtacgccg gtccaggaga gggtggcggg
     1621 gatagacttc atgatgcgcc tgagccggac ggccgctgct gaaagactgc cggtgtacct
     1681 cctcggcgcg aaggaagaag cggtgaaggg ggcggctgaa accctgaagg aacagtaccc
     1741 ggatcttgtc atcgcaggct ggaggaacgg gtatttcgat ccctgcgacg cctccgtcgc
     1801 cggaaccgtc cgggacagcg
//
LOCUS       DAOTHD010000465         1818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_76480_length_1818_cov_3.201361, whole genome shotgun sequence.
ACCESSION   DAOTHD010000465 DAOTHD010000000
VERSION     DAOTHD010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76480_length_1818_cov_3.201361"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="P5219_08040"
     CDS             <1..403
                     /locus_tag="P5219_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRX26748.1"
                     /translation="FAVTWGVLAFILNFIPTVGSIVASIPPVLVALVQFYPSYMPAVI
                     TALVLLTIQVTIGNFITPKVMGDRLNLSPVVVLISLLFWSLIWGVVGALISTIIAAII
                     KIICENIPSLNFISVMMGSGKVYQKEFEERA"
     gene            441..>1818
                     /locus_tag="P5219_08045"
     CDS             441..>1818
                     /locus_tag="P5219_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HRX26749.1"
                     /translation="MKKTAVFAAAALFLFTAAALPSPAAGPSLAPLNPAFLRDRALPG
                     GGEAELRRSVFFAGERRPSGKRPSPLNLDHLRGRGETVRYLAARTFREDAAFPSRFDL
                     REQGKLSPIRDQDPYGTCWTFAAMAALESPLLPGETADFSEWHLAYWAYNDTPDGFPA
                     FTVYDGVGLFDQGGDDWKAMAVLARGTGPVNESDLPYGGPLPSEKPRLPRQKLLTSAL
                     YMPYPAVEIWRDGYPYLAVEEVKDALMNRGAVSIGMMADFNEDTWNGETGAFFYSGPR
                     GANHAVNIVGWDDHFPRERFATRPAGDGAWIVRNSWGTSFGEAGYFYMSYEDTSLDSG
                     IVYLASSAGEYGIIHQYDPLGWIRSVSPLETPAAGTPAWMANVFTTEESQVLRAVSFY
                     VPDYGTEYEITIGVLSGPTPADKESMPLRGQRGVLEKPGYHTVALGKQVYLPRGTRFS
                     VEVKVTAPE"
BASE COUNT          344 a          576 c          538 g          360 t
ORIGIN      
        1 ctttgcggtg acctggggcg tactggcctt catcctgaac ttcatcccca cggtgggttc
       61 catcgtggcc tccattcctc cggtcctagt ggcactggtg cagttctacc cgagctacat
      121 gccggcggtc atcacagccc tggtcctcct gaccatccag gtgaccatcg gcaacttcat
      181 cacgcccaaa gtcatggggg accggctgaa cctcagtccc gtcgtggtgc tcatctccct
      241 gctcttctgg agccttatct ggggtgtggt gggggctctc atatccacca tcatcgccgc
      301 catcatcaag atcatctgcg agaacatacc ctccctgaac ttcatcagcg tcatgatggg
      361 gtccggcaag gtctaccaga aggagttcga ggaaagggcc tgagtcccgg aaaaaagacc
      421 agcgaaagga agtgtccttc atgaaaaaaa ctgccgtttt tgcggcagcc gctctttttc
      481 tgttcaccgc tgccgctctg ccgtcccctg cggcaggtcc gtcccttgcc cccctcaacc
      541 cggcctttct ccgtgaccgg gctcttcccg gcggcggaga agcagagctc cggagaagtg
      601 tttttttcgc gggggaaagg cgtccctccg gaaaacggcc gtctcctctg aacctggatc
      661 atctccgggg caggggagaa actgtccggt atcttgcggc ccggaccttc agagaagacg
      721 cagcctttcc ctccaggttc gacctccggg aacaggggaa actgtccccc atacgggacc
      781 aggatcctta cggcacctgc tggaccttcg ccgccatggc ggctttggaa tcgcctcttc
      841 tgccgggaga gacagccgat ttctcggagt ggcacctggc ctactgggcc tataatgaca
      901 ctcccgacgg attcccggcc ttcaccgtgt atgacggggt cggcctcttc gatcagggag
      961 gggatgactg gaaggccatg gccgtcctcg cgagaggcac cggtccggtg aacgaatcgg
     1021 atcttcccta cggcggcccc ctgccgtcgg aaaaaccccg gcttccgcga cagaagcttc
     1081 tcaccagtgc cctgtacatg ccctatccgg ccgtggaaat ctggagggac ggctacccct
     1141 atctcgccgt ggaggaggtc aaggacgccc tgatgaaccg gggggctgtg tccatcggca
     1201 tgatggctga cttcaacgaa gacacctgga acggggaaac gggagccttt ttttacagcg
     1261 gtccccgggg agcgaaccat gcggtgaaca tcgtcgggtg ggacgaccat ttccccaggg
     1321 agcgattcgc gacccggccg gcaggcgacg gggcgtggat cgtccggaac agctggggaa
     1381 catctttcgg cgaggcgggg tacttctaca tgtcctacga ggacacgagc ctcgacagcg
     1441 gcattgtcta ccttgcctca tccgcggggg aatacggtat catccaccag tacgaccctc
     1501 tgggatggat acggtccgtt tctcccctcg aaacgcccgc tgcaggcact ccggcctgga
     1561 tggccaacgt cttcacgacc gaggagagcc aggttttgag ggcggtctct ttttacgtgc
     1621 ccgactacgg aacggaatat gaaatcacga taggggtcct gtccggccca acccctgccg
     1681 acaaggagtc catgcccctc cgggggcagc ggggcgtgct ggaaaaaccg gggtaccaca
     1741 ccgtcgccct tggaaaacag gtctacctcc cccggggaac gcggttctca gtggaagtga
     1801 aggtaaccgc cccggaat
//
LOCUS       DAOTHD010000466         1814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_76728_length_1814_cov_3.402501, whole genome shotgun sequence.
ACCESSION   DAOTHD010000466 DAOTHD010000000
VERSION     DAOTHD010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76728_length_1814_cov_3.402501"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1563)
                     /locus_tag="P5219_08050"
     CDS             complement(<1..1563)
                     /locus_tag="P5219_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRX26750.1"
                     /translation="MMKSILLGTAALLVFSLLSSPARAAEGLIPLEDFFRNPDRSGFQ
                     ISPDGNRISWRESWERRMNIFVRKIGEETATRITSATERDILAYFWAGNDRILYLQDS
                     GGDENYHLYAVDAGGSEARDLTPFKGVRVSIVDRLEDIEDEVLIGMNRRDPRLFDVYR
                     LNIATGELTLLEENPGDIEGWMTDHDGKLRVAIRTDGVNRSLLYRDREDYPFRTLLTT
                     DFRESVSPLFFTFDNKKLYVSSNLGRNTSAIFVFDPEKAAHEELVFEHPEVDVYALFR
                     SKKRKVITGTGYTTDRFRYHFFDEERAALQKELESRLPGYEVTVTSMNKEEDKVLLHA
                     GSDRTQGAYYFYDMKTKDFRKLADLSPWLPGERLAPMKPVQYTARDGLTIHGYLTLPA
                     GRDGKNLPVVINPHGGPWARDSWGYDSEVQFLANRGLAVLQMNFRGSTGYGRAFWEAS
                     FKQWGRAMQDDITDGVKWLIEQGIADPDRIGIYGGSYGGYAVLAGLAFTPELYACGVD
                     YVGVSNIFTLLET"
     gene            complement(1567..>1814)
                     /locus_tag="P5219_08055"
     CDS             complement(1567..>1814)
                     /locus_tag="P5219_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRX26751.1"
                     /translation="TGLHRDILEWLEKTAPENPSYAHHRTGEDNGDAHLKRIVVHHQA
                     ILPVTGGRLDLGPWEQCFYAEFDGRRRKRVIVKIMGE"
BASE COUNT          311 a          552 c          559 g          392 t
ORIGIN      
        1 cgtctccagg agggtgaaga tgttggacac ccccacgtag tccactccgc atgcgtagag
       61 ttccggcgtg aaggcgagcc cggcgaggac ggcgtacccg ccgtaggagc cgccgtagat
      121 gcctatcctg tcaggatcgg cgattccctg ctcaatgagc catttcactc cgtcggtgat
      181 gtcgtcctgc atggcccgcc cccactgctt gaagcttgcc tcccagaatg ccctgccgta
      241 gcccgtggaa ccccggaagt tcatctggag caccgcgaga ccacggttgg cgaggaactg
      301 gacctcgctg tcgtatcccc acgagtcccg ggcccaggga cctccgtggg ggttgatcac
      361 cacggggagg ttctttccgt cccttcctgc cggaagggtg agatatccgt ggatcgtcag
      421 gccgtcccgg gcggtgtact gcaccggctt catgggggcg agcctctccc cgggaagcca
      481 ggggctgagg tcggccagct tccggaagtc ctttgtcttc atgtcgtaaa agtaataggc
      541 cccctgggtc cggtcgctcc cggcgtggag gagaaccttg tcttcctctt tgttcattga
      601 cgtcaccgtg acctcatacc cggggaggcg ggactcgagc tccttctgga gggcggcccg
      661 ctcctcgtcg aagaaatggt accggaaacg gtcggtggtg tatcctgttc ccgtgatcac
      721 tttccgtttt ttcgaccgga acagggcgta cacgtccacc tccgggtgct cgaagaccag
      781 ttcttcgtgt gccgcctttt cggggtcgaa gacgaaaatg gccgatgtat ttctccccag
      841 gttcgaggag acgtagagct ttttgttgtc gaaggtgaag aagagagggg aaacactttc
      901 ccggaaatcg gtggtgagga gcgtccggaa ggggtagtcc tccctgtccc ggtagaggag
      961 ggaccggttg accccgtcgg tcctgatggc cacccgaagt tttccatcgt ggtcggtcat
     1021 ccatccctcg atgtcgccgg ggttttcctc gaggagggtc agttctccgg tggcgatgtt
     1081 cagccggtac acgtcgaaga gccgggggtc cctcctgttc atgccgatga gaacctcgtc
     1141 ctctatgtcc tcgagacggt ccacgatgga cacccggact cccttgaagg gagtgaggtc
     1201 cctcgcctcc gaccccccgg cgtccacggc gtacagatgg tagttttcgt ccccgcccga
     1261 gtcctgcagg tagagaatcc ggtcgttgcc ggcccagaaa taggccagga tatcccgttc
     1321 cgtcgctgac gtgatccgtg tcgccgtctc ctctccgatt ttccggacga aaatgttcat
     1381 tcgccgctcc caggactccc gccaggaaat gcggttcccg tctggggata tctggaatcc
     1441 gcttctgtcg ggattccgga aaaagtcctc caggggaatg agtccctccg ccgcccgggc
     1501 aggagaggag agaagggaga agaccagaag agccgccgtt ccaagaagaa tggatttcat
     1561 catcgcttat tcccccatga tcttgacgat cacgcgcttt ctgcgcctgc cgtcgaattc
     1621 tgcatagaaa cactgttccc acggtccgag gtcgagcctg ccgcccgtca caggcagaat
     1681 cgcctggtgg tggaccacga tccgcttcag gtgagcgtcc ccgttgtcct cgccggtgcg
     1741 gtgatgggcg taggacggat tttcgggggc ggttttctcc agccactcca ggatgtcccg
     1801 gtggaggccc gttt
//
LOCUS       DAOTHD010000467         1813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_76799_length_1813_cov_3.366894, whole genome shotgun sequence.
ACCESSION   DAOTHD010000467 DAOTHD010000000
VERSION     DAOTHD010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76799_length_1813_cov_3.366894"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="P5219_08060"
     CDS             <1..267
                     /locus_tag="P5219_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26752.1"
                     /translation="TLAFSGATFVIYEGPGGSEVRKITLTSSKHPLDGGLAVGMDRRQ
                     VLSLLGHPNDLVKGEEVYRVNKASGIRIKYENYRVKAVTAGVLN"
     gene            282..>1813
                     /locus_tag="P5219_08065"
     CDS             282..>1813
                     /locus_tag="P5219_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HRX26753.1"
                     /translation="MVVPPVPGTSFFPVNREEMEARGWAGLDFLLISGDAYVDHPSFG
                     PAVIARVLESRGFRVGILPQPDWKSREPFLAMGRPRLAALVTAGNLDSMLNRLTASKK
                     SRSRDSYSPGGKAGLRPDRATVVYGSRVRECWKDLPLVIGGVEASLRRFAHYDYWSDD
                     VRRSVLIDSGADLLVYGMGEKQITEIAALLDRGVPVEKIRSVRGTMYRSAELPEDGKT
                     VAVPSWEEVSSDRRKFAEAFRLQYPEQDPFHGKAVAQRFGNVWTVQNPPPRPLTMDEM
                     DQVYGYPYTRTYHPMYEKDGGVPAIEEVRFSLTSHRGCFGSCSFCAIHYHQGRIIQAR
                     SHGSLVEEARLLTTLPDFKGYIHDVGGPTANFRIPACDDQLVRGACRNRQCLFPAPCP
                     KLRADHSDYLELLGKLRALPGVKKVFIRSGIRFDYLMADKKTPFLEELCAHHVSGQLK
                     VAPEHVSPGVLSVMGKPGCEVYGKFMEAYAEMNRKLGKKQYLVPYFISSHPGAELKDA
                     VRL"
BASE COUNT          384 a          507 c          559 g          363 t
ORIGIN      
        1 acccttgcgt tcagcggggc gacctttgtg atttatgaag gtccgggcgg ttccgaagtg
       61 cggaagatca ctctcacctc gtcgaagcat ccccttgacg gcgggcttgc cgttggaatg
      121 gacaggcggc aggttctgtc tctcctgggg catccgaatg atctcgtgaa gggagaggaa
      181 gtctaccggg tcaacaaggc ctccggcatt aggataaagt acgaaaatta cagagtgaaa
      241 gcagtaaccg caggggtgct caattgacgg attattctgc aatggtggtc ccgcccgttc
      301 ccgggacttc cttctttccc gtgaacaggg aagagatgga agcgagagga tgggcggggc
      361 ttgactttct tcttataagc ggagatgcct acgttgacca tcccagtttc ggccctgcag
      421 ttatcgccag ggttcttgag agcaggggat tcagggtggg cattcttccc cagcctgact
      481 ggaagagcag agaacccttc cttgccatgg gccgcccccg tctcgccgcc ctggtgacgg
      541 cagggaacct ggattccatg ctcaacaggc taacggcatc gaagaaaagc aggagccggg
      601 acagctattc cccgggaggg aaagcaggac tgcggcccga cagggcgacc gttgtctacg
      661 gaagccgggt gcgggaatgc tggaaggatc ttcccctggt gatcggtggc gtggaagcga
      721 gcctccgccg ctttgcccat tatgattact ggtctgacga cgtccggcgg tccgtcctga
      781 ttgacagcgg ggcggatctc ctggtgtacg gaatgggaga aaagcagatc accgaaatag
      841 ctgccttgct tgaccgtggt gttccggtgg aaaaaatccg gtccgtccgg gggaccatgt
      901 accgttccgc tgaacttccg gaagacggga agaccgtggc ggtgccttcc tgggaagaag
      961 tctcctccga caggaggaag tttgccgaag ccttcaggct ccagtatccc gagcaggacc
     1021 ccttccatgg gaaagcggtc gcccagcgct tcgggaatgt ctggaccgtc cagaaccctc
     1081 ctccccgtcc tctcaccatg gatgaaatgg accaggtcta cggctatccc tataccagga
     1141 cctaccatcc catgtacgaa aaggatgggg gggtgccggc catcgaggaa gtccgcttca
     1201 gcctcacgag ccacaggggc tgtttcggga gctgttcttt ctgcgccatt cactaccacc
     1261 agggaagaat catccaggcg agaagccacg gttccctggt ggaagaagcg cggctcctca
     1321 ccactctgcc ggatttcaag gggtacattc atgacgtggg aggccccacg gcgaactttc
     1381 ggattcccgc ctgcgatgat cagcttgtcc ggggagcatg ccggaaccgg cagtgcctgt
     1441 tccccgctcc gtgcccgaaa ctccgggctg accacagcga ctatctcgaa ctgctcggaa
     1501 agctccgtgc cctgccgggg gtaaaaaaag tcttcatcag gtcgggaatt cgctttgact
     1561 acctcatggc ggacaaaaaa acgccatttc tggaagagct ctgcgcccac cacgtgagcg
     1621 ggcagctcaa ggtggcgccg gagcacgtgt cgccgggagt tctgtccgtc atgggaaaac
     1681 cgggatgcga agtgtacggg aagtttatgg aagcctacgc cgaaatgaac aggaagctgg
     1741 gaaagaagca gtatctcgtt ccctacttca tttcttccca tccgggagcg gaactgaagg
     1801 atgcggtgcg cct
//
LOCUS       DAOTHD010000468         1813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_76814_length_1813_cov_2.902730, whole genome shotgun sequence.
ACCESSION   DAOTHD010000468 DAOTHD010000000
VERSION     DAOTHD010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76814_length_1813_cov_2.902730"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="P5219_08070"
     CDS             <1..482
                     /locus_tag="P5219_08070"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRX26754.1"
                     /translation="SLFNDRLGMAMAEARRCRKKVGVIFLDLDNFKKVNDAMGHSAGD
                     LLLVGAAERMQRTLRDTDTLARIGGDEFAFLFPMVEDRPEVEAVAARILEALSVPFPV
                     EGREFVISGSLGICLFPDDGDDGQTLMKRADTAMYEAKRAGKNTWRHWTGFPCSANG"
     gene            510..1661
                     /locus_tag="P5219_08075"
     CDS             510..1661
                     /locus_tag="P5219_08075"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRX26755.1"
                     /translation="MFPSFSYEMPTRVEFGRGAALRTGEEAGRIGGTRVLLISDEGVR
                     RAGLLDRITESLDRASVPWDLFLDVEPNPKDRNVHAAAAAVSETGADCLVAVGGGSPI
                     DCAKAVAIVAAAGGKVREYEGRFTTSVLPLPIIAVPTTAGTASELTFGSVITDTAAKF
                     KFTVKSPLLAPKVALADPEMTATMPKSLTAFTGMDALTHAIEAYTCRAATPFSDAPAI
                     VAMELIGKYLRRAWKDGSDMEAREGMLLGSLLAGVAFSHSDVAAVHCLAEALGAMYDA
                     PHGACNSIALPAIMEFSMDACVDRYARVAACLGLAFSTPREGAEKAVEEVKRMAVDLE
                     LPSLSVFGVREEDFDEIAAKSEANSSNPDNPKIMKQNDYIAVLRKLTGK"
BASE COUNT          355 a          534 c          608 g          316 t
ORIGIN      
        1 ggtccctgtt caacgaccgg ctcgggatgg ccatggccga ggccaggcgg tgccggaaaa
       61 aagtcggggt catctttctc gacctggaca atttcaagaa agtgaatgac gccatggggc
      121 attccgcggg agatctgttg cttgtcggcg ccgcggagag gatgcagcgc accctcaggg
      181 atacggacac cctggccagg atcggcgggg atgaatttgc gtttcttttc ccgatggttg
      241 aggaccggcc cgaggtggag gccgtggcgg cccggattct ggaagccctc tcggttcctt
      301 ttccggttga gggccgggag ttcgtcattt caggcagcct ggggatctgc ctctttccgg
      361 acgatggaga cgacgggcag acgctcatga agagggcgga caccgctatg tacgaggcaa
      421 agcgggcggg gaagaatacc tggcgccact ggacagggtt cccctgttcc gccaacgggt
      481 aaatttacag gaagaaagga tggaaaaaaa tgttcccttc attcagctat gaaatgccca
      541 ccagggtgga gttcggccgg ggggcggcgc tccgtacggg agaagaggca gggaggatcg
      601 gcgggacgcg ggtgctgctg atttccgacg aaggggtccg cagggcggga ctgctcgacc
      661 gcatcacgga gagccttgac cgggcttccg ttccctggga cctgtttctc gacgtggagc
      721 ccaacccgaa ggaccggaat gttcatgccg ctgctgcggc agtgtccgaa acgggggcgg
      781 actgtctcgt ggcggtgggg ggaggcagcc ccatcgactg cgccaaggcc gtggccatcg
      841 tggcggcggc ggggggcaag gtcagggagt acgagggacg gttcacaacc tccgtcctcc
      901 ccctcccgat catcgccgta cccaccacgg cgggaacggc gagtgaactg accttcggct
      961 ccgtcatcac cgacacggcg gcgaaattca agttcaccgt caagagtccc ctgctggccc
     1021 cgaaggtggc cctggcggat cctgaaatga cggccaccat gccgaagagc ctcaccgcgt
     1081 tcacggggat ggatgccctg acccacgcca tcgaggccta tacctgccgg gccgccaccc
     1141 ccttctccga cgctcccgcc atcgtggcca tggaactcat cgggaaatac ctccgcaggg
     1201 cctggaagga cggctccgac atggaggcca gggaaggaat gctcctcgga agcctccttg
     1261 cgggagtggc cttcagccat tccgacgtgg cggcggtcca ctgtcttgcc gaggccctgg
     1321 gtgccatgta tgacgctcct cacggagcct gcaactccat cgcccttccg gccataatgg
     1381 agttttccat ggatgcctgc gtcgaccggt acgccagggt tgccgcctgc ctcggcctgg
     1441 cgttctccac accccgggag ggagcggaaa aggcagtgga ggaagtgaag aggatggccg
     1501 ttgaccttga gctgccctcc ctttctgttt tcggcgtcag ggaggaggat ttcgacgaaa
     1561 tcgccgcgaa atcggaggcg aattcctcga acccggacaa tccgaagatc atgaaacaga
     1621 acgactacat cgccgtgctc cggaaactga cgggaaaata gcaccggagc gggaaagacg
     1681 gcatgccgga cagaaaaaac ggcccggggc ggaaaagccc cgggccggtg catgacgcgg
     1741 cagtgaaatt atctctttgg cggttcccag cgcttcgcca tgttctgggt caggcggtcg
     1801 atgacgatgg cca
//
LOCUS       DAOTHD010000469         1812 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_76889_length_1812_cov_2.639158, whole genome shotgun sequence.
ACCESSION   DAOTHD010000469 DAOTHD010000000
VERSION     DAOTHD010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76889_length_1812_cov_2.639158"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /locus_tag="P5219_08080"
     CDS             complement(<1..131)
                     /locus_tag="P5219_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V"
                     /protein_id="HRX26756.1"
                     /translation="MAIYMNNGATTWPKPECVARAMADFLTGRGANLARGSASERDM"
     gene            463..>1812
                     /locus_tag="P5219_08085"
     CDS             463..>1812
                     /locus_tag="P5219_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26757.1"
                     /translation="MNTPVHSRVVFGMLCIFLFLFSALPAQARNPADFEVILFENPGC
                     EGENQLAFSYDNDLPNLNRFNIPGGGKWNDRISSLAIGKNARVILYQHSDFRGPSISF
                     EADGTGSLLIDDLHLLGWGDTVTSAKIRLRVVPAPDEVLIFEHVNFDGAMLSPSAGGN
                     YPDLRQWTISGGKTWNDKISSLKIGKDALLCVYADIDFGGPSVVFSGDGQGTVEIISL
                     HGTGWEDAVSSMKVRARGWTPESDEASGGSQGSEGTGGTGGGQSEGSGQGSGTGGSGE
                     TSAGDGQEEGGEEETDPLENEIVFFKDADFQGEHRIYAVGENLPDLRQLSFWDSTGNW
                     NDEISSLKIGANAQAYVCTHINYEGPCVQHRGNGTGVTRIDNLHRSGWGDRISSIKIR
                     EKDWNPEDDIPEPEADEVIFFEHVDYGGKHLVVKANRHELDLTKVFLENSNTSWNDKI
                     "
BASE COUNT          425 a          513 c          517 g          357 t
ORIGIN      
        1 ctcatgtccc gctcggaggc gcttccccgg gcaaggttgg cgccccggcc ggtgaggaag
       61 tcggccatgg cccgggctac gcactccggc ttcggccagg tggtggcgcc gttgttcatg
      121 taaatcgcca atccgatcat ctcctggtta tactatttat aatcaatatc atacactctt
      181 ttgagaaatt ttcattaccc tggaagggat ttcgcgctat gccgttttcc tttcacaaaa
      241 ataaaagtgg aatatcttcc cggactttct cccggcgttc agtgagaaat tctcaccgga
      301 aaaagccgcg cttttcctgg ctgcgcagtg gagaaaacac agacgacggt atgagggttc
      361 cggttgaccc ccctccggtt tcattttata attcaaaaca gttggaatag tatatcgaca
      421 tggagaatga tacccgccag acacaaagga ggagatgctt ccatgaacac accggtccat
      481 tccagggtgg ttttcggtat gctgtgtatt ttccttttcc ttttctccgc ccttcctgca
      541 caggcccgga acccggcgga tttcgaggtt atcctgttcg agaatcccgg ctgcgaagga
      601 gaaaaccagc tggccttctc ctacgacaac gatctcccca atctcaacag gttcaacatt
      661 cccggcggcg ggaaatggaa cgacaggatc agctccctgg cgatcggaaa aaatgcccgg
      721 gtcatcctgt accagcattc cgatttcagg ggccccagta tcagcttcga ggcagacggc
      781 accggcagcc tgctcatcga cgacctgcac ctccttggct ggggggacac ggtgacctcg
      841 gcaaaaatcc ggctgagggt tgtcccggca ccggatgagg tgctgatctt cgaacatgtc
      901 aacttcgacg gagctatgct ctctccttcc gcgggaggaa attacccgga cctcaggcaa
      961 tggaccattt caggcggcaa aacctggaac gacaaaatca gttccctgaa gatcgggaag
     1021 gacgcccttc tctgcgtata cgccgacatc gacttcggag ggccgtccgt tgtcttcagc
     1081 ggcgacgggc aggggaccgt tgaaatcatc agccttcacg gtaccgggtg ggaagacgcc
     1141 gtctcctcca tgaaggtccg tgcccggggc tggaccccgg aaagcgatga agcttcaggc
     1201 gggtcccagg gatcggaagg aacaggagga accgggggag gccagtctga aggctccggc
     1261 cagggcagcg ggaccggagg atccggagaa acatccgccg gagatggtca ggaggaaggc
     1321 ggagaggagg aaaccgatcc cctggagaac gaaatcgtct ttttcaagga tgcggacttc
     1381 cagggcgaac acaggatata tgccgtcggg gagaacctgc ccgatctccg gcagctttcc
     1441 ttctgggact ccaccggaaa ctggaacgac gagatcagtt ccctgaagat aggggccaac
     1501 gcccaggcct acgtctgcac ccacatcaac tacgaaggtc cctgcgtcca gcaccggggg
     1561 aacggaaccg gtgtcacccg gatcgacaat ctccaccgca gcgggtgggg ggacaggatc
     1621 tcttcgatca agatcaggga gaaagactgg aacccggagg acgacatccc ggaacccgag
     1681 gctgacgagg tcatcttctt cgaacatgtg gactacggag gaaagcatct tgtcgtcaag
     1741 gcaaaccggc acgagctcga tctcacgaag gttttcctgg agaactcgaa caccagctgg
     1801 aacgacaaaa tc
//
LOCUS       DAOTHD010000470         1810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_76988_length_1810_cov_3.122507, whole genome shotgun sequence.
ACCESSION   DAOTHD010000470 DAOTHD010000000
VERSION     DAOTHD010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76988_length_1810_cov_3.122507"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="P5219_08090"
     CDS             <1..1145
                     /locus_tag="P5219_08090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRX26758.1"
                     /translation="VHVNSTRAGGGVAEILSSMVPLSRELGLDVSWEVIEGDEPFFAC
                     TKTLHNSLQGMAGGLSEGQVASYTATNERNAEKLKESLEGADYVFIHDPQPAALLGMM
                     PGRKGKWIWRCHIDVSAPNRSVWRFVKKQVEGYDASIFSLPDFAQPLSHPQFLITPSI
                     DPLSEKNIPLSKAEVEKTYRELGVPRDKPVILQVSRFDRFKDPLGVIESYRIAREHAD
                     IRLVLAGGGAADDPESGEVLSAVREAAAGDPDISVLVLPPDTHRAINALQSGADVIVQ
                     KSTREGFGLTVSEGMWKRKPVIGGDVGGIRLQVINRFNGFRVRSPEGAAIRIRYLLTH
                     RWKRERMGVNARQYVLENFLITGQLRNYLSLLVTLDGGEGEWERWI"
     gene            1127..>1810
                     /gene="otsB"
                     /locus_tag="P5219_08095"
     CDS             1127..>1810
                     /gene="otsB"
                     /locus_tag="P5219_08095"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HRX26759.1"
                     /translation="MGAVDIGHPGLGLSDLEGFFGSFGGVSPLLVSDYDGTLAPFRRE
                     RSEAVPSEETKDLLQRILEAGGEFLLLTGREASEAASLLGVPAEIWGCHGWQRRSPGG
                     DTVTVPLSAAEERALLLLPDLFRAFPPEAVERKPVSAALHWRERPEVLEQYGKFEESI
                     LERASQAGLERLPFDQGIEFRLPSCTKGVAMKRILAARSFRDPVCYLGDDVTDEDAFR
                     VLDGRGAGIL"
BASE COUNT          358 a          515 c          582 g          355 t
ORIGIN      
        1 ttgttcacgt aaactccacc agggccggag ggggagtggc ggaaattctc tcttccatgg
       61 tccccctctc ccgggagctc ggccttgacg tctcctggga ggtcatcgag ggagacgaac
      121 ccttcttcgc ctgcacgaaa acgctgcaca actcactgca ggggatggcg ggaggacttt
      181 ccgagggaca ggttgcctcc tacacggcga cgaatgaaag aaacgccgaa aagctgaagg
      241 agagcctgga aggtgcggat tacgttttca tccacgaccc gcagccggcg gccctgctgg
      301 ggatgatgcc cggacggaag gggaagtgga tctggcggtg ccatatcgac gtcagcgccc
      361 cgaaccggtc ggtgtggcgg ttcgtgaaaa aacaggtgga gggctatgac gcctcgatct
      421 tctcactgcc cgatttcgcc cagcccctgt ctcatccgca gtttctcatc acacccagca
      481 ttgatccgct cagcgagaag aacattcccc tctcgaaggc ggaagtggaa aaaacgtacc
      541 gggagcttgg agtgcccagg gacaagcctg tcatcctcca ggtttcccgt ttcgaccggt
      601 tcaaggaccc tcttggggtc attgagagct accggatcgc gagagaacac gcagatatcc
      661 ggcttgttct cgcagggggc ggggcggcgg atgatcccga gtcgggagag gtcctttccg
      721 cagtccgcga ggcggccgca ggggatcccg atatttccgt ccttgtcctt ccgccggata
      781 cccatcgggc catcaatgcc ctccagtccg gtgcagacgt gattgtgcag aaatccacca
      841 gggaaggctt cggccttacg gtgagcgagg gcatgtggaa aagaaaaccg gtcataggcg
      901 gtgacgtcgg gggcatacgg ctgcaggtca tcaaccggtt caacggtttt cgcgtgcggt
      961 ctcccgaggg tgccgccatc aggatccggt acctcctgac ccaccgctgg aaaagagaga
     1021 gaatgggcgt gaacgccagg cagtacgttc ttgagaattt cctgattacc gggcagcttc
     1081 ggaactacct ttcccttctg gtgaccctgg acggaggaga aggagagtgg gagcggtgga
     1141 tataggacat cccggcctgg ggctttcgga cctggaagga ttcttcggtt ccttcggcgg
     1201 agtcagcccg ctgcttgtga gcgattacga cggtaccctc gcccctttcc gaagggaaag
     1261 gagcgaggcc gttccttccg aagaaacgaa ggaccttctc caaagaattt tggaagcggg
     1321 aggagagttt ctccttctca ccgggagaga ggcgtcggag gctgcttccc ttcttggagt
     1381 gccggcggaa atatggggat gtcacggatg gcagcgccgg tcacccggag gtgatacggt
     1441 caccgtaccc ctttcggccg cggaggagcg ggcccttctt ctgctcccgg acctgttccg
     1501 cgccttccct cccgaagcgg tggaaaggaa gcccgtttcc gccgccctgc attggagaga
     1561 acggcccgag gttcttgaac agtacgggaa gttcgaggag tccatccttg aaagggcttc
     1621 gcaggccggt cttgaaagac ttcccttcga ccaggggatt gagttccgcc ttccttcgtg
     1681 caccaagggg gtggccatga aacggatact ggcggcccga agcttcaggg accccgtctg
     1741 ctacctcggg gatgacgtga cggacgagga cgcattccgg gtgcttgacg gccggggtgc
     1801 aggaattctc
//
LOCUS       DAOTHD010000471         1808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_77127_length_1808_cov_2.701084, whole genome shotgun sequence.
ACCESSION   DAOTHD010000471 DAOTHD010000000
VERSION     DAOTHD010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77127_length_1808_cov_2.701084"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="P5219_08100"
     CDS             complement(<1..456)
                     /locus_tag="P5219_08100"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRX26760.1"
                     /translation="MKRMFVRCVIKGKRETGLVENGIIYRLEGDMFGESSRGPALGSV
                     DEAERFLPPVEPSKVAAVGLNYRDHAIEVGLPIPEEPLLFLKPSTTVIAHGDPVVYPA
                     HMTERVDYEAELAVIIGRKCSRVEPGEAEDCILGYTCANDVTARDLQSKD"
     gene            752..1483
                     /gene="phoU"
                     /locus_tag="P5219_08105"
     CDS             752..1483
                     /gene="phoU"
                     /locus_tag="P5219_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRX26761.1"
                     /translation="MFWKKNGKVESLLYSADRAKILSMVREMGSMASEAFSNAVRSLE
                     ERNDALADEVIRSDDIIDDLEASIDQECLSSIAMRQPVREDLRFVFAVLKIITDLERI
                     GDQGVNIAKKAKILNRYPLLKPLVDIPKMKVIATEMVADSLKAFEEDDTALAEAICLR
                     DEELDILYENIFDELLLIIGNKPQGDQATAQCAAGLLWVARHLARIGDHATNVAERVF
                     FMVKGERLKPLLEEKRKALSESKGG"
     gene            1545..>1808
                     /locus_tag="P5219_08110"
     CDS             1545..>1808
                     /locus_tag="P5219_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRX26762.1"
                     /translation="MKKAILAVLAVCIALGAGAAFAADYPTKAVTIIVASNAGGGTDT
                     MARLFAKFAEKYFPQPFVINNIDGAGGQRGFDALARAKKDGYTI"
BASE COUNT          395 a          555 c          509 g          349 t
ORIGIN      
        1 gtcttttgac tggaggtccc tggcggttac gtcgttggcg caggtgtacc ccaggatgca
       61 gtcctccgct tctccgggtt caacacggga acacttcctc ccgatgatga cggcgagctc
      121 ggcttcgtag tccacccgct ccgtcatgtg tgcgggatac accacggggt cgccgtgggc
      181 aatgaccgtt gtggaaggct tcaggaagag caggggttcc tccggtatgg gaaggccgac
      241 ctcgatggcg tggtcccggt agttgagtcc caccgctgct accttggacg gctccacggg
      301 agggagaaac cgttcagcct cgtccaccga accgagagcc ggtccccggg aagattcgcc
      361 gaacatatcc ccttccagcc ggtaaatgat gccgttctcc accagccctg tctccctttt
      421 tccctttatg acgcaccgca cgaacattct tttcattatt ctctcctccg gcgtgctttc
      481 catggatttt ctggctccac taccgagacg attctaccat aggggaacat gatcctgaaa
      541 aaggaaggtt catcccctcc gcccgctgaa aatcaggaat cagcttcttg acaatcctcc
      601 cggtatgtcg tagtttgtaa cattatcgtt acacgcctgt aacctcgctg aggacatttt
      661 ttcccgctat cctgccaagg ccccccgggg gccccacaaa agatgacggt tcccgcaggc
      721 aggctgtacc gacgagggag ggactgcaga gatgttctgg aagaaaaacg ggaaggttga
      781 atcccttttg tattcagccg acagggcgaa aatcctttcc atggtccgag agatggggtc
      841 catggcgagc gaggcattct ccaacgcggt ccgctcgctg gaagagcgga acgatgccct
      901 ggcggacgag gtcatacgat ccgatgacat catagacgat ctcgaggcgt ccatcgacca
      961 ggaatgcctc agctccatcg ccatgcgcca gcccgtccgg gaggatctcc gcttcgtttt
     1021 cgccgtgctg aagatcatta ccgacctgga gcgcataggc gaccagggtg tgaacatcgc
     1081 gaaaaaggcg aagatcctga accgctatcc tctcctgaaa ccactagtgg acattccgaa
     1141 gatgaaggtc atcgccaccg agatggtggc cgattccctc aaggccttcg aggaggacga
     1201 caccgccctc gccgaggcca tctgcctccg ggacgaggag ctcgacatcc tctacgagaa
     1261 cattttcgac gaactccttc tcatcatcgg caacaagccc cagggagacc aggccaccgc
     1321 acagtgcgcc gccgggctcc tctgggtcgc gcggcatctc gccaggatag gcgatcacgc
     1381 caccaacgtg gcggaacggg tgttcttcat ggtcaaggga gagcggctga aaccccttct
     1441 cgaggagaag agaaaagccc tctccgaaag caaaggaggc tgacgccgga gaacgacacg
     1501 aaaccacgga tcaggggaac tcactttcga aaggagacga tactatgaaa aaagcaattc
     1561 tggctgttct cgcagtgtgc attgccctcg gagcgggggc ggccttcgcc gcagactacc
     1621 cgacgaaggc cgtcaccatc attgtggcgt ccaatgcggg cggcggcacc gacaccatgg
     1681 cgaggctctt cgccaagttc gccgagaagt acttccccca gccctttgtg atcaacaaca
     1741 tcgacggcgc cggcggccag aggggcttcg acgccctcgc acgggcaaaa aaggacggct
     1801 acaccatc
//
LOCUS       DAOTHD010000472         1804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_77370_length_1804_cov_3.161235, whole genome shotgun sequence.
ACCESSION   DAOTHD010000472 DAOTHD010000000
VERSION     DAOTHD010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77370_length_1804_cov_3.161235"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="P5219_08115"
     CDS             <1..653
                     /locus_tag="P5219_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX26763.1"
                     /translation="GYSFAGFAEKGGEIAPLLSKTTVNLVLLDIALPGFNGLEGFRRM
                     RTNFPRVDFIILSSEKSPDVVRGAICSGAFDYLIKPFEWERLTRALAAYSEYYHGLVG
                     RDMPWRQEDLDRLLGFRKRISGTPEGAPKGFQETLLIRLGKLLQESRKPLSAADAGRM
                     LGISRSSARRYLELLVQREEVAVDFELTTVGRPQKLYFATGPVVKKEQNHPKMSNN"
     gene            773..1255
                     /locus_tag="P5219_08120"
     CDS             773..1255
                     /locus_tag="P5219_08120"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRX26764.1"
                     /translation="MQRFGKLVSLLILPLIVGIVYSSLKSYIYNETPIWTFEVSLFLY
                     GSFFMLGSAYCHLEKKHVAVDVLSHYLPPKWKRILAIFSEAVVLFVALTIIWVSVPGA
                     WRSTLMRERSTHQTPFNPEVWWYRWIIPISCALISWQAFKDMLALILGRPEKNGGEAS
                     "
     gene            1271..>1804
                     /locus_tag="P5219_08125"
     CDS             1271..>1804
                     /locus_tag="P5219_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRX26765.1"
                     /translation="MARELYPLLMFAALFVLLAGGVPIAFSLTAVSIGFAYVLWGPGA
                     LNIVVSAAWGSMNNFVIIAVPLFIYMAYILQKTNVVEDLYSAFFKWSGGLRGGLAVAT
                     VVVGAVLGAVSGVVAAGVIGLGLIGLPQMLKHGYNRRMALGCVMGAGTLGQLIPPSTN
                     MVLYGCVTGVSIGGLFAG"
BASE COUNT          340 a          559 c          512 g          393 t
ORIGIN      
        1 agggctattc cttcgccggt ttcgcagaaa aagggggaga gatcgctccg cttctttcga
       61 aaacgacagt gaacctcgtg cttctcgata tcgcccttcc cgggttcaac ggcctggagg
      121 gattccggcg gatgcggacg aatttcccga gggtggattt catcatcctg tcttcagaaa
      181 aatcacccga cgtcgtccgg ggcgccatct gttccggagc cttcgattac ctgatcaagc
      241 cctttgaatg ggagcggctc accagggcgc tggcggccta cagcgagtat taccacggtc
      301 tcgtgggaag ggatatgccc tggcggcagg aggacctcga caggcttctc ggcttcagga
      361 aacggatttc aggcactccg gagggcgctc ccaagggatt ccaggagacc ctgctcatcc
      421 gcctggggaa actgctgcag gagtcgagga agcctctttc ggcggcggat gcaggcagga
      481 tgctcggcat ttcccgctcc agcgcccgca ggtacctgga gcttctcgtc cagcgggagg
      541 aagtcgcggt tgatttcgaa ctgaccacgg tggggcgtcc ccagaaactg tattttgcca
      601 cggggcccgt tgtcaaaaag gagcaaaacc acccaaaaat gtcaaataat tgaacgatat
      661 tctcccggtg cttatactga agcactgtcg gcatttcaag caattcccgc atttccagga
      721 cggctccgcc acctgaaaaa caggagagac aatatcgtga ggaggacggc ccatgcagcg
      781 tttcggcaag ctagtgtcac ttctgattct gccgctcatc gtcggcatcg tctacagttc
      841 cctgaagtcg tatatttaca acgaaacccc catctggact tttgaagtgt cccttttcct
      901 ttacggctca ttcttcatgc tcggctccgc ctactgccac ctcgaaaaga agcatgtggc
      961 ggtggacgtg ctcagccatt accttccccc gaagtggaag cgcatcctgg ccatcttttc
     1021 cgaggcggtg gtgctcttcg tcgccctgac gatcatctgg gtgagcgtcc ccggagcctg
     1081 gcgctccacc ctcatgaggg aacggtccac ccaccagacg cccttcaacc ctgaggtctg
     1141 gtggtaccgc tggatcatcc ccatttcctg cgccctcatt tcctggcagg cattcaagga
     1201 catgcttgcc ctcattctcg gccgccctga aaaaaacggc ggcgaagcaa gctgacggag
     1261 gagaatcgcc atggcccgtg aactgtaccc tcttctcatg ttcgccgccc ttttcgtgct
     1321 ccttgcggga ggggttccca tcgccttctc cctcaccgcc gtctccatag gattcgccta
     1381 cgtcctctgg gggcccggag ccctcaacat cgtcgtatcc gccgcatggg gctccatgaa
     1441 caatttcgtc atcatcgcag tcccgctgtt tatctacatg gcctacatcc tccagaagac
     1501 caacgtggtg gaggacctct attccgcctt cttcaaatgg tccgggggac tcaggggcgg
     1561 tctcgccgtg gccacagtgg tggtgggggc cgtcctcggg gcagtgtccg gcgtggttgc
     1621 ggcaggagtc atcggcctcg ggctgatcgg cctgccccag atgctcaagc acggatacaa
     1681 caggcgcatg gccctgggat gcgtcatggg ggcgggaacc ctgggacagc tcattccgcc
     1741 gagcacgaac atggtgctct acgggtgcgt caccggcgtc tccatcggcg gccttttcgc
     1801 cggc
//
LOCUS       DAOTHD010000473         1804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_77385_length_1804_cov_2.752430, whole genome shotgun sequence.
ACCESSION   DAOTHD010000473 DAOTHD010000000
VERSION     DAOTHD010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77385_length_1804_cov_2.752430"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="P5219_08130"
     CDS             complement(<1..930)
                     /locus_tag="P5219_08130"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRX26766.1"
                     /translation="MTAGVFLYTFYELSSIVIRIVMLCYIPQRHSPTTAMSWLLVIYF
                     WPWPGFLLYIAFGSTALPRERIERHEALLRTLTADRATFRRILDDESHWLPEELVRFG
                     ALGKKLGDMCITTGNSFVLIDREEEFLKSLCADIDGAKESVHLLYYIFALDSMTERLF
                     ASLEGAAARGVECRLLVDALGAKPFLKKDASRLRTAGVRVEKALPQGLLRRTATTARY
                     DLRNHRKIAVIDGRIGYTGSHNVTEPSYGGKAGGRLWKDLSFRLTGPVVLQLQGVFME
                     DWYVETRELLPTERGFPRPERTGRAILQTVPSGP"
     gene            complement(927..1688)
                     /locus_tag="P5219_08135"
     CDS             complement(927..1688)
                     /locus_tag="P5219_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRX26767.1"
                     /translation="MRLVLYNIRYGTGTGWDYHFPFPFSGCLRKTEGRFRRITDFISK
                     LKPDIVGLVEADSGSYRQNGECQAELMAARLKAESVFACKYHHESLVSRAPLLKSQGN
                     AVVTRLPILSTREHYLSRGVKRTLLEVEFESFTLFLAHLSLGYGTRKIQIGEVAERCR
                     QAKKPVILAGDGNIYAGEKELRPLFRKGLFRNANDGNMPTYPSGLPALALDFVLYSEG
                     IEMKDFRVPRVRYSDHLPLVCDFTPEGRSGEALAS"
BASE COUNT          346 a          538 c          580 g          340 t
ORIGIN      
        1 cggtccgctg ggcaccgtct ggagaatcgc ccgtcccgtc cgttcaggcc tggggaagcc
       61 ccgctccgtg ggaagaagtt ccctggtctc cacgtaccag tcctccatga agaccccctg
      121 gagctggagc accaccggtc ccgtgagcct gaaggacaga tccttccaca gccgtcctcc
      181 cgccttgccg ccgtaggaag gctccgtgac gttgtgggat cccgtatacc cgatgcggcc
      241 gtcgatgacg gcgatcttcc ggtggttcct caggtcatac cgggccgtgg tggccgtcct
      301 gcggagcagt ccctggggga gggccttctc cacccggacg ccggcggtcc ggaggcggga
      361 ggcgtccttc ttcaggaagg gcttggcccc cagggcgtcc acaaggagcc tgcactccac
      421 ccccctggcc gccgcccctt caagggaggc gaaaaggcgc tccgtcatgg aatccagggc
      481 gaagatgtag tacaggaggt gcaccgattc cttcgctccg tcgatgtcgg cacagaggct
      541 tttcaggaac tcctcttccc tgtcgatgag cacgaaggag ttgcccgtgg tgatgcacat
      601 gtcccccagt tttttcccca gcgccccgaa acggacaagc tcttcgggca gccagtggga
      661 ttcgtcgtcg agaatcctgc ggaaggtggc cctgtcggcc gtgagggtgc ggagcagggc
      721 ctcgtgccgc tcgatcctct cccgcggcag ggcggtggaa ccgaaggcga tatagagcag
      781 aaagcccggc cacggccaga aatagatgac gagaagccat gacatggccg tggtgggact
      841 gtgtcgctgg gggatgtagc agagcatgac gattcggatg acgatggagg aaagttcgta
      901 aaaggtgtag agaaaaactc cggccgtcat gaggccagcg cctccccgct ccggccttca
      961 ggagtgaagt cgcagacgag ggggagatgg tcggaatacc tgacccgggg gacccggaag
     1021 tccttcatct caatgccctc gctgtagagc acgaaatcga gggccagggc cggcagtccg
     1081 ctgggatagg tgggcatgtt cccgtcgttg gcgttcctga acagcccctt gcggaagagg
     1141 gggcggagct ccttctcgcc ggcgtagatg ttgccgtcac cggcgagaat gacgggcttc
     1201 ttcgcctggc ggcaccgttc cgccacttcg ccgatctgga ttttcctcgt tccgtacccc
     1261 agggaaagat gggccaggaa gagggtaaaa gactcaaact cgacctcgag gagggtccgc
     1321 ttgacccccc tgctcaggta gtgctcccgg gtcgagagaa tcggcagcct ggtcaccacg
     1381 gcgttcccct gggatttcag caggggggcc ctggagacga gagactcgtg gtggtacttg
     1441 caggcaaaaa cggactcggc tttcagccgg gccgccatga gttccgcctg gcattcgccg
     1501 ttctgcctgt aggagcccga gtcagcctcc acaagcccca cgatgtcggg cttcagcttc
     1561 gatataaagt cggtgatccg ccggaaccgt ccctcggttt tccggagaca gccggaaaag
     1621 gggaagggaa aatggtagtc ccatcccgta ccggtcccgt agcggatgtt gtagagtaca
     1681 agtcgcatgc agatcctccg ggtaaagaaa taatcagtga ttatacagca aaacgcgggt
     1741 ctttcatcgg tttttcggcc tgttttttcg tatcggcgga aaaaccgatg aaagacccgc
     1801 gttt
//
LOCUS       DAOTHD010000474         1803 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_77433_length_1803_cov_3.204805, whole genome shotgun sequence.
ACCESSION   DAOTHD010000474 DAOTHD010000000
VERSION     DAOTHD010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77433_length_1803_cov_3.204805"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="P5219_08140"
     CDS             complement(<1..746)
                     /locus_tag="P5219_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26768.1"
                     /translation="MDAMRVGRGLSAGLVFLLLLGGGPSCAQFHGRGAEVRVVSPVQI
                     VSEPSRMHTISIEVTNRTNRQERFREDVILPKGWSMVIPMTSFVLPPQASTARLLSFQ
                     IPINAAEGRAKIQYSVQSDRDPSIRGSMEYEVVVLAVAKTDLMVENPPASLMAGEEYE
                     FSARLVNSGNIPRTFLIRAKNTDPNSPALAAPSEVSLGPGEGAAISVSGKIDPGFRGT
                     VTVIQITAEIEKDGKKEEAVSTTVLLEVVPA"
     gene            complement(762..1586)
                     /locus_tag="P5219_08145"
     CDS             complement(762..1586)
                     /locus_tag="P5219_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26769.1"
                     /translation="MKHRVLVNGLCLACILLAGLCDSLSAGVNLNVDGTLTRSFTLDP
                     GGKTRGEIVVQNTGDEESELKIFLQDYLHYADGRALFQKTGTVGRSNASWITMTPNQA
                     VIPAKGSLTINFTLSVPPDPKLKGTYWSLLMIEPVPKSLLTPGKQENDVDIQVVTVVR
                     LAVQMITTIGTSGEAKLSFRNRSLTKEKSGRFLTLDLENTGERLLTPHVWTELFNKKG
                     QSSGKIDGSRHRIYPGCSARFVLDLSKLAPGGYKAVVVADNGDDNVFGANYTLELK"
     gene            complement(1583..>1803)
                     /locus_tag="P5219_08150"
     CDS             complement(1583..>1803)
                     /locus_tag="P5219_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26770.1"
                     /translation="KLWVRRSGSGHGAGWIRSGLGFVPVGESPVLLFSGTGDRMRVPL
                     QLRIGGITPRTPGATIRTSLIFTVVRTR"
BASE COUNT          336 a          578 c          510 g          379 t
ORIGIN      
        1 gcgggcacga cctccaggag gacagtagtg gagacggcct cctctttttt cccgtccttc
       61 tcgatttccg ccgttatctg gatgacggtg acagtccccc ggaatccggg gtcgatcttt
      121 cccgagacgg aaattgccgc cccttccccg ggcccgagtg aaacttccga tggagccgcc
      181 agagctggtg agttcggatc cgtgttcttc gcccgtatca ggaacgtgcg ggggatattg
      241 cccgaattga ccagccgggc ggaaaactcg tattcctccc ccgccatgag cgatgccgga
      301 gggttctcca ccatcaggtc agtcttcgcc accgcgagaa cgaccacctc atattccatc
      361 gagccccgta tggacggatc ccggtcagac tgcaccgaat attggatttt cgcccgtccc
      421 tctgcggcat tgatgggtat ctggaacgac aggaggcggg ccgtcgaggc ctggggaggc
      481 agcacgaagg acgtcatggg gatcaccatt gaccagcctt tcggcaggat gacgtcttcc
      541 cggaaccgtt cctgcctgtt cgtccggttg gtaacctcga tactgatggt gtgcatgcgg
      601 ctcggctcgg acacgatctg gaccggtgaa acaacccgga cctccgctcc cctgccgtga
      661 aactgggcac aggaaggccc tcctcccaac agaaggagaa agacaagccc cgccgacaat
      721 cccctgccga cccgcatggc gtccatgact gttacctccc gttacttgag ctccagcgtg
      781 taattcgccc caaacacgtt gtcgtccccg ttgtccgcaa cgacaacggc cttgtaccct
      841 cccggcgcaa gcttcgacag gtcgagcacg aaccgtgccg aacaccccgg gtagatccgg
      901 tgcctgctcc cgtcgatctt cccgctcgac tgcccctttt tgttgaacag ttcggtccac
      961 acatggggtg tcagcagccg ctcccccgtg ttttcgagat cgagggtcag gaaacgaccg
     1021 cttttttcct ttgtgaggct gcgattccgg aaactgagct tcgcctcgcc tgacgtgccg
     1081 atggtggtaa tcatctgcac ggcaaggcgg accacggtga caacctggat gtccacgtcg
     1141 ttttcctgtt ttccgggcgt caggaggctt ttcgggacag gttcgatcat gagcaggctc
     1201 cagtaggttc ccttgagttt cggatcagga ggaacggaaa gggtgaaatt gatcgtcagg
     1261 gatccctttg ccggtatcac cgcctggttg ggtgtcatgg ttatccagga ggcgttcgac
     1321 cgtcccacgg tcccggtttt ctggaagaga gcccgtccgt ccgcgtagtg aagatagtcc
     1381 tggagaaaaa tcttgagctc gctttcctcg tctccggtgt tctggacgac gatctccccc
     1441 ctggtcttcc cccccggatc gagggtgaac gacctggtga gcgttccgtc cacattcagg
     1501 ttgacgccgg cagagaggct gtcgcacaat cctgccagca gaatgcaggc caagcacaat
     1561 ccattcacga gcaccctgtg tttcatcgcg ttctcaccac cgtaaaaatc aggctggtcc
     1621 gtatcgttgc cccgggcgtc cggggagtga tccccccaat tctgagctga agggggaccc
     1681 gcatcctgtc ccccgttccg gaaaaaagaa gcaccgggct ctcgccgacg ggaacaaacc
     1741 ccagtccgct gcggatccag cctgccccat gcccgctgcc gctccggcgc acccagagct
     1801 tca
//
LOCUS       DAOTHD010000475         1803 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_77436_length_1803_cov_2.967391, whole genome shotgun sequence.
ACCESSION   DAOTHD010000475 DAOTHD010000000
VERSION     DAOTHD010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77436_length_1803_cov_2.967391"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..906)
                     /locus_tag="P5219_08155"
     CDS             complement(154..906)
                     /locus_tag="P5219_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRX26771.1"
                     /translation="MTVTKDDLLYGALTLRQPAEGPRVNVDTILLSAYVRDTFPRTGG
                     RILELGCASGAVSLILALRFPDAALTGLEIQEDLAALAEENAAINGLSGRISVVRGDL
                     RNSGTLFPPQSFDCVAMNPPYGEPGRGRPSASRADRAARQGIWCSPGDVASAARYLLK
                     HRGRMYVVFRAGRTAELLASLSERDVEPKRIRFVHPLPGRKASVVLVEALRGGKPGMT
                     VEPPLFIEDGSGNYTEELLSAYTKEGLPCRSQ"
     gene            complement(903..>1803)
                     /locus_tag="P5219_08160"
     CDS             complement(903..>1803)
                     /locus_tag="P5219_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26772.1"
                     /translation="NTSINPTGSRSILSSKREALAGELRQLAEMWEEQYPYSPEEAPS
                     GEEGEAASAAVRRLERELRALGNVEWGALSEDQSLAARTAFLSEQLGDVGAAIGELRG
                     IITETDLHVGVLFGEALENINARFNSLFCRLFGGGEAWLRLQEPDPDGPGGEEEKKSS
                     PAWDAGVEIVARPPGKHLQNLAQLSGGEQTLTAIAYLFASMEVAGVPLAVLDEVDAAL
                     DESNLLRFGDLAKEYSSPPGQGKGIQLLVMTHRRATMERADILYGVTLAEPGLSRVVG
                     MKVEDWAEPEEREGRKLSGAVRR"
BASE COUNT          303 a          596 c          574 g          330 t
ORIGIN      
        1 gggcgtgata ggagacaagc cgggaggaga tgccgaaatg ccggagcagg ggcagggatg
       61 tcctggtgtc ctcgcaggcg atcacgtcgg cttccttcag catccggacg gcccggggcg
      121 acatgtcttc caggtttccc accggggtgg ggattactgc gagcggcatg gcagtccctc
      181 cttcgtatag gcggacaaaa gctcctccgt gtagttcccg ctgccgtctt cgataaaaag
      241 gggaggctcc acggtcatgc ccggttttcc tccccggaga gcttccacca ggaccaccga
      301 ggctttcctt cccggcaggg ggtggacaaa gcggattctc ttcggctcca cgtcccgctc
      361 cgaaagagac gccaggagct ccgctgtcct tcccgcccgg aagaccacgt acatccttcc
      421 ccggtgcttc agcaggtacc gggccgccga cgccacatcg ccgggggagc accagattcc
      481 ctgccgtgcc gcccggtcgg cccgggacgc gctgggcctt ccccggccgg gctccccgta
      541 gggagggttc atggccacgc agtcgaagga ctggggggga aagagcgtcc cggaattcct
      601 gagatccccc cgcaccacgg atattctccc cgacagtccg ttgatggcgg cgttctcttc
      661 agcgagggcg gccaggtctt cctggatctc gagtcccgtg agggcagcat cgggaaaccg
      721 aagggcaagg atcaaggata ccgctcctga agcgcatccg agctccagga tcctgccgcc
      781 cgtccgcgga aaagtatccc ggacgtaggc cgacaggagg atggtatcca cgttcaccct
      841 ggggccttcg gccggctggc ggagagtcag ggctccgtag aggagatcgt ccttcgtgac
      901 ggtcatcgcc gcaccgcccc ggatagtttt ctgccttccc tctcctccgg ctcggcccag
      961 tcctccacct tcatgcccac gaccctggac agccccggct ccgcaagggt gacgccgtag
     1021 agaatgtcgg ccctctccat ggtggcccgg cggtgggtca tgacgagaag ctggattccc
     1081 ttgccctgtc ccggaggcga tgagtattct ttggcaaggt cgccgaatct caggaggttt
     1141 gattcgtcca gggccgcatc cacctcgtcg aggaccgcaa ggggaacacc ggccacctcc
     1201 atggaggcga aaaggtaggc gatggcggtg agggtctgct cgcctccgga aagctgtgcc
     1261 aggttctgga ggtgcttccc gggaggacgg gccacgatct ccaccccggc gtcccatgcc
     1321 ggagaggact tcttttcctc ctccccgccc ggtccatcgg gatcgggttc ctgaaggcgg
     1381 agccaggcct ctcctccgcc gaacagcctg cagaagagag aattgaagcg ggcgtttatg
     1441 ttctccaggg cctcgccgaa caggactccc acgtggaggt ccgtctcggt gatgatcccc
     1501 cggagttccc ctatggccgc tccgacgtcc ccgagctgct cggaaagaaa tgcggtccgc
     1561 gcggcgaggg actggtcctc ggaaagggca ccccactcca cgttcccgag agcccggagt
     1621 tcccgctcca gcctgcggac cgccgcagag gcggcttctc cctcctctcc ggaaggagct
     1681 tcttccgggc tgtaggggta ctgttcttcc cacatctcgg caagctgccg gagttccccg
     1741 gcgagggcct cccttttcga ggagaggatc gagcgtgaac ctgttggatt gatcgaagta
     1801 ttt
//
LOCUS       DAOTHD010000476         1799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_77673_length_1799_cov_3.165711, whole genome shotgun sequence.
ACCESSION   DAOTHD010000476 DAOTHD010000000
VERSION     DAOTHD010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77673_length_1799_cov_3.165711"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="P5219_08165"
     CDS             complement(<1..922)
                     /locus_tag="P5219_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRX26773.1"
                     /translation="MARREILGVRVKLASPERIRELSSGEVKKPETINYRTLRPEKDG
                     LFCERIFGPTKSYECACGKYKRSGPKFKGIICDRCGVEVTDNRVRRERMGHIELAAPV
                     VHIWYLRGIPSRLSLLLGCTTKDLEKVVYFAPTRQREAAYKVVMEGRRIDLARKNDVL
                     SESEVRIHSHYDPKFKAEEAFRIVSVDDVPVNEDDLLSAAQVSRFKTEYGDESFKVET
                     AFSLVTIPNDPSCAEGAVISATDLERLRKRHGDTFEVERAMAGNQEAFVVTAAVHLPF
                     EKGDVLSESEFRLYSQKFPGIGSIVARECGS"
     gene            complement(922..>1799)
                     /locus_tag="P5219_08170"
     CDS             complement(922..>1799)
                     /locus_tag="P5219_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRX26774.1"
                     /translation="LPVEDMPYLPDGTPVDVVLNPLGVPSRMNLGQVLETIMGFVAFQ
                     KGWKVATPVFEGAKEKEIFDLLEQLSKDKYPELTSDGMITLYDGRTGDAFEKKVTVGC
                     MYMLKLIHLVDDKIHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWALEGYGAANIL
                     QEMLTVKSDDIRGRLKTYERIVKGQNLTKPGVPESFRVLVKELQGLGLDVEVKYSDGQ
                     IGELLMDDDEDEPPVGRYSGPRERPVFEEKEPEEKEKSDSVFGEEEMEEIVPEEMLFG
                     EELPDGMSIEEGEED"
BASE COUNT          296 a          560 c          538 g          405 t
ORIGIN      
        1 gggaaccgca ttcccgcgcc acgatcgacc cgattccggg gaatttctgg ctgtagagcc
       61 ggaactcgct ctcggaaagg acgtcgccct tttcaaaggg aagatggacg gcagcggtga
      121 ccacgaaggc ctcctggttt ccggccatgg cccgctccac ttcaaaggtg tcgccgtggc
      181 gcttccggag gcgctcaagg tctgtggccg atatgaccgc accctctgcg caggaggggt
      241 cgttcggtat ggtgacgagg gaaaaggctg tttccacctt gaagctttcg tcgccgtact
      301 ccgtcttgaa ccgggagacc tgggccgcgg acagaaggtc atcctcgttc acggggacat
      361 cgtccacgga aacgatgcgg aaggcttcct cggccttgaa cttggggtcg tagtggctgt
      421 ggatccggac ctcgctctcg gagagcacgt catttttcct ggcaaggtcg atccgtctgc
      481 cctccatgac caccttgtag gcggcctccc gctggcgggt gggggcgaag tagaccacct
      541 tctccaggtc cttggtggtg cagcccagca gcaggctgag cctgctgggg atgccccgga
      601 ggtaccagat gtgcaccacc ggcgcggcaa gctcgatatg gcccatgcgc tcacggcgga
      661 cccggttgtc ggtgacttcc acgccgcacc gatcgcagat gatgcccttg aacttggggc
      721 cgctgcgctt gtattttccg caggcgcact cgtagctctt tgtggggccg aagatgcgct
      781 cgcagaagag cccgtccttt tcaggccgga gggtccggta attgatggtc tcgggcttct
      841 tcacctctcc gctggagagt tcccgtattc tttccgggct tgcgagcttg acccgcaccc
      901 cgagaatttc ccttcgggcc atcagtcttc ctccccttct tctatggaca taccgtcagg
      961 aagctcttcg ccgaagagca tttcctcggg aacgatctct tccatttctt cctctccgaa
     1021 cacggagtcg ctcttctctt tttcttcagg ttccttctcc tcgaaaacgg ggcgctccct
     1081 cgggccggaa tatcgtccga cgggtggctc gtcctcgtcg tcgtccatga gcagttcgcc
     1141 tatctggccg tcgctgtact tcacttccac gtcgaggccc agtccctgga gttccttgac
     1201 gagaaccctg aaactctccg gtacgcccgg cttggtgagg ttctgcccct tgacgatccg
     1261 ttcgtaggtc ttgagccttc cccggatatc gtcagacttc acggtaagca tttcctggag
     1321 gatgttcgca gcgccgtacc cttccagggc ccagacttcc atctcgccga acctctggcc
     1381 gccgaactgg gcctttccgc ccaggggctg ctgggtgatg aggctgtagg ggccgatgga
     1441 ccgggcatgg atcttgtcgt ccacgaggtg gataagcttg agcatgtaca tgcagcccac
     1501 ggtgaccttc ttctcgaagg cgtcccccgt ccggccgtca tagagagtaa tcatgccgtc
     1561 ggaggtgagc tccggatatt tatccttgct gagctgctcc agcaggtcga agatttcctt
     1621 ctccttggcc ccttcgaaca cgggagtggc cactttccag cccttctgga aggcaacgaa
     1681 acccatgatg gtctcgagca cctggccgag gttcatgcgg ctgggaacgc cgagaggatt
     1741 gaggaccacg tccaccggcg tgccgtcggg gaggtagggc atgtcctcca cggggagga
//
LOCUS       DAOTHD010000477         1795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_77924_length_1795_cov_2.913793, whole genome shotgun sequence.
ACCESSION   DAOTHD010000477 DAOTHD010000000
VERSION     DAOTHD010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77924_length_1795_cov_2.913793"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="P5219_08175"
     CDS             <1..193
                     /locus_tag="P5219_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRX26775.1"
                     /translation="SMSGSLLGTAVITLVTELFRPISQYRMLIYGAVLVVIMVLRPEG
                     IMGNREFVSWVKAPFRRRA"
     gene            193..966
                     /locus_tag="P5219_08180"
     CDS             193..966
                     /locus_tag="P5219_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX26776.1"
                     /translation="MSAFFELSGVTKNFGGVQAVSNMTFSLEKGELAGLMGPNGAGKT
                     TVFNLVTGVYDPDEGSILFKGENLVPLKTYQVTARGVARTFQNLRLFQRSTVLENVMT
                     AAQHHVPYSFIEGITHLGRWRSGEKAIRDKSMALLERVGLADRAEQAAGTLPYGHQRR
                     LEIARALALDPELLLLDEPAAGMNAEEVTSLNVLIREIHRDFSLTTLVIEHHMDLVME
                     ICPHIICMNFGAKIAEGTPEEIQNHPEVLKAYLGEEEDE"
     gene            963..1715
                     /locus_tag="P5219_08185"
     CDS             963..1715
                     /locus_tag="P5219_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX26777.1"
                     /translation="MTGTGERNVLLEVKDLHVSYGAIRALSGVSLKVYEGEIVSVIGS
                     NGAGKSTLMNAIMAMVRREKGDVFLGGAALEARSYKVVQQGVSLSPEGRKVFAPLTVL
                     ENLKMGAFPRGKDSKVDEDLEWVFSLFPRLKERIAQYAGTLSGGEQQMLAIGRALMAR
                     PKVLLLDEPSLGLAPIIIRDIFKELRRINNEGVTILLVEQNARQALLLSHRGYVLQTG
                     HMVQEGPSEELLANADIKAAYLGGGKAPKGLS"
BASE COUNT          377 a          507 c          558 g          353 t
ORIGIN      
        1 ctccatgagc ggctcgctcc tgggaaccgc ggtgattacc ctggtgacgg aactcttccg
       61 tcccatttcc cagtaccgca tgctgattta cggagcggtc cttgtcgtta tcatggtcct
      121 tcgccccgag ggaatcatgg gcaaccgtga attcgtctcc tgggtgaagg cgcccttcag
      181 gaggcgtgcc tgatgagcgc attcttcgaa ctttccggcg taacgaagaa tttcggcggc
      241 gtgcaggccg tgagcaacat gaccttctcc ctggagaagg gagagctggc aggcctcatg
      301 ggaccgaacg gagcgggaaa gaccacggtg ttcaacctgg tcacgggagt gtacgacccc
      361 gatgagggaa gcatcctttt caagggggaa aatcttgtcc cgctgaaaac ctaccaggtt
      421 acggcgcggg gggtggcccg aacattccag aacctcaggc tgttccagcg ttccaccgtc
      481 ctggagaacg tgatgacggc agcccagcac cacgtgccct attcctttat cgaagggatt
      541 acccacctgg ggcggtggcg ctccggtgag aaggcgatac gggacaaaag catggccctt
      601 ctggagcgcg tggggttggc cgaccgggcc gagcaggcgg cgggaaccct tccatacggc
      661 caccagcgga ggctcgagat tgcacgggcc ctggccctgg atcccgaact gcttcttctt
      721 gatgaaccgg ccgccggcat gaacgcggag gaagtgacct ccctgaatgt cctgatacgg
      781 gaaattcaca gggatttcag cctgaccacc ctcgtcatcg aacatcacat ggaccttgtc
      841 atggaaatct gcccccacat catctgcatg aacttcgggg caaagatcgc cgagggaacc
      901 ccggaggaga ttcagaatca ccccgaagtg ctcaaggcct atctcggaga ggaggaagac
      961 gaatgaccgg gaccggcgaa agaaacgtcc tccttgaggt gaaggacctg catgtcagct
     1021 acggggcgat cagggccctt tcgggagtgt ccctgaaagt gtacgaagga gagatcgtca
     1081 gcgtcatcgg ttccaacggc gcggggaaat cgacccttat gaacgccatc atggccatgg
     1141 tcaggcggga gaaaggggat gtattcctcg ggggtgccgc cctcgaggcg cggagctata
     1201 aagtggtgca gcagggggtt tccctgagcc cggagggacg gaaagtcttc gcccccctca
     1261 ctgtcctgga aaatttgaaa atgggggcct tccccagagg aaaggactca aaggtggacg
     1321 aggaccttga atgggtgttt tccctttttc cgaggctgaa ggaacggatc gcccagtatg
     1381 cgggcaccct ttccggggga gagcagcaga tgcttgccat cggcagggcc cttatggcca
     1441 ggccgaaagt gctcctcctc gatgagccgt cccttgggct ggctcccata atcatcaggg
     1501 acatattcaa ggagctgcgg cggataaaca atgagggggt caccatcctg ctggtggaac
     1561 agaacgcccg gcaggctctt cttctctccc acaggggcta tgtcctgcag acagggcaca
     1621 tggtccagga aggcccgtcg gaggagcttc tcgcaaacgc ggacatcaag gcggcctatc
     1681 tcgggggcgg aaaagcgccg aagggccttt cctgaagttt ttttgccgtc gttcaaagag
     1741 cccgcagggt tttcccctgc gggctctttg tcggcttttc cctttatttc cctat
//
LOCUS       DAOTHD010000478         1795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_77932_length_1795_cov_2.624138, whole genome shotgun sequence.
ACCESSION   DAOTHD010000478 DAOTHD010000000
VERSION     DAOTHD010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77932_length_1795_cov_2.624138"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="P5219_08190"
     CDS             <1..269
                     /locus_tag="P5219_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26778.1"
                     /translation="GLAPSLTCCLSCGKPLGDAVWNAEGLNCPECARRSGGSRLADGQ
                     RKLLVSAAECPFDEVKRLFPFPGNDREFWKDGCKRMKEFFGFLK"
     gene            473..1366
                     /locus_tag="P5219_08195"
     CDS             473..1366
                     /locus_tag="P5219_08195"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HRX26779.1"
                     /translation="MNFQEIIFRLERFWAEQGCVVQQPYDIEVGAGTMNPATCLRVLG
                     PEPWRVGYVEPSRRPADGRYGENPNRLQHYYQYQVIIKPAPANIIDLYLQSLAALGIE
                     PAAHDIRFVEDDWESPTVGAWGLGWEVWLDGMEVTQFTYFQQVGGIDMDLVPAEITYG
                     IERIAMFVQKVENVYDLQWVDDMKYGDIHHKGEVEYSHYNFEIADTKMLFQLFGMYEA
                     EALRVLDKGFVLPAYDYVLKCSHTFNLLDARNAISVTERTGYIGRIRLLAGKCCAAYA
                     AQRRAMGHPFRGKFAGESARR"
     gene            1372..>1795
                     /locus_tag="P5219_08200"
     CDS             1372..>1795
                     /locus_tag="P5219_08200"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HRX26780.1"
                     /translation="MVLKNILLEIGTEEIPSRFLPDTLEFLEKTARDDLAAARIGFKS
                     LSAFATPRRIALLVRDADERQSDLVNTFKGPAWNAAFDVNGNPTRAAHGFARSRGVSI
                     EQLAPMEVDGVRYACAEVSEPGGRTLDILPELLPGLIRK"
BASE COUNT          360 a          567 c          542 g          326 t
ORIGIN      
        1 ggggcctggc cccttcgctc acttgttgtc tctcctgcgg gaagcccctc ggtgatgccg
       61 tatggaacgc ggaagggctg aactgcccgg aatgcgcccg ccgcagcgga ggctcccgcc
      121 tggcggacgg gcagagaaag cttcttgtct cggcggcgga atgccccttc gacgaggtga
      181 agcggctctt tccgtttccc ggaaacgaca gggaattctg gaaggacggc tgcaaaagaa
      241 tgaaagagtt ttttggcttt ttgaaataac gatgcggtcc caaagtgctg ttcccccttc
      301 aaaaagcgga agcggattgt cattccgagg agcgtagcga cgaggaatct cgccctgaag
      361 gcttctctgt atccggccca tggtttccgt gtctgcgatt tccttaggag gggctcatgt
      421 ttcattttgg gacagtagcg aaaaaggcat ttccattgag gagggatcag gcgtgaattt
      481 ccaggaaatc atctttcggc tcgaacggtt ctgggcggag cagggatgcg tcgtccagca
      541 gccctatgac atagaggtgg gcgcaggcac catgaacccc gccacgtgcc tccgggtcct
      601 cggacccgag ccgtggaggg tgggctacgt ggagccgtcc cggcggcccg ccgacggcag
      661 gtacggcgag aaccccaacc gcctgcagca ctactaccag taccaggtca tcatcaagcc
      721 ggcgccggcc aacatcatcg acctctacct ccagagcctc gccgccctgg gcatagaacc
      781 ggccgcccac gacatccgct tcgttgagga cgactgggaa tcccccacgg tgggcgcctg
      841 gggactgggc tgggaagtct ggctcgacgg catggaggtc acccagttca cctacttcca
      901 gcaggtgggg ggcatcgaca tggacctcgt gcctgccgag atcacctacg gcatcgaacg
      961 catcgccatg ttcgtccaga aggtggagaa cgtctacgac ctccagtggg tggacgacat
     1021 gaaatacggc gacatccacc acaagggtga agtggagtac tcccactaca acttcgagat
     1081 cgccgacacg aagatgcttt tccagctttt cggcatgtac gaggcggagg ccctccgcgt
     1141 cctggataag ggattcgtgc tgcctgccta cgactacgtc ctgaaatgct cccacacctt
     1201 caacctcctc gacgcccgga acgccatcag cgtcaccgag cggacggggt acatcggcag
     1261 gatccggctt ctcgcgggca aatgctgtgc cgcctatgcc gcccagagac gggccatggg
     1321 ccatcctttc cgcggaaaat tcgccggcga gtcggccagg aggtaatcgt catggtgctg
     1381 aaaaacatcc ttctcgaaat cggaaccgaa gaaatcccct cacgcttcct gccggacacc
     1441 ctcgaatttc tcgagaagac cgccagggac gacctggctg ccgcaaggat aggcttcaag
     1501 agcctgtccg ccttcgccac gccccgcagg atcgcccttc tcgtgcggga cgccgacgag
     1561 cggcagagcg accttgtcaa taccttcaag ggcccggcat ggaacgccgc cttcgatgtc
     1621 aacggcaatc ccaccagggc ggcccacggc ttcgccagga gccggggcgt ctccatcgaa
     1681 cagcttgccc cgatggaagt ggacggcgtg cgctacgcct gcgccgaagt ctccgagccc
     1741 ggggggagga cccttgacat cctcccggag cttctgcccg gcctcatcag gaagc
//
LOCUS       DAOTHD010000479         1792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_78119_length_1792_cov_2.451353, whole genome shotgun sequence.
ACCESSION   DAOTHD010000479 DAOTHD010000000
VERSION     DAOTHD010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78119_length_1792_cov_2.451353"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /gene="lpxI"
                     /locus_tag="P5219_08205"
     CDS             <1..233
                     /gene="lpxI"
                     /locus_tag="P5219_08205"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201639.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HRX26781.1"
                     /translation="AGGLLSGAPGVVVKMMRPDQDIRFDLPTVGTGTLRAMAAAGLTC
                     LAVEAGRTIILDRPDFASLAESLSIAVEGIIP"
     gene            230..1378
                     /locus_tag="P5219_08210"
     CDS             230..1378
                     /locus_tag="P5219_08210"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRX26782.1"
                     /translation="MTPAGRGIFFSCGEVSGDGYAAAVLDALRRRGFAGSLFGMGGPL
                     AAGAGAEIVSDSRALHLMGVTDVLPAIPRLLRLKKNLVDLILSRRPGAVLLIDSPDFH
                     LPLAASLRGSGWRGPIVYAAPPTVWAWRPWRVKNLRRDMDLCLPLFQFEHEYLLRAGV
                     NSAWKGHPLVDEFSAPLPEEADPKRIALLPGSRRSEVFRLLPPLVECASLLKDRGYRP
                     VFSIAPGMPEDLRNRLRNVLKGHETWEGPGRDLLASSAAAAGASGTVAVEAMMLGRFM
                     AVLYRGGFLSWLAWKTLVKTPRISIPNLLAGEELYPELIQGRATGRNAFSALLGFLED
                     ERAAEHIRVGLLSARRAMGLPGAVDFWAERLEALVSVQSAFQGEGRDP"
     gene            1375..>1792
                     /locus_tag="P5219_08215"
     CDS             1375..>1792
                     /locus_tag="P5219_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cdisaccharide synthetase"
                     /protein_id="HRX26783.1"
                     /translation="MKSVLLLANGPGELWGWVRPVAAELKRRSRKVSLLLLPCQFASG
                     EERRVAESLAPDELRGPSGLAGTLRDALFLGRGAEAVLQLGGDLAWGRIAARAGRAPL
                     LCYAYGRKNGLPRCDRVFTAFPAMAEAMGDRVYVAGD"
BASE COUNT          257 a          592 c          608 g          335 t
ORIGIN      
        1 gggcgggggg cctgctttcg ggagctccgg gcgtcgtggt gaagatgatg cgtcccgacc
       61 aggatatccg gttcgatctc cccaccgtgg ggacgggaac cctccgggcc atggcggcgg
      121 cggggctgac ctgccttgcc gttgaggccg gccggaccat catcctggac cgccctgact
      181 tcgcctccct ggcggagagt ttgtccatcg cagtggaggg catcatcccg tgacgccggc
      241 cgggcggggg atttttttca gctgcggcga ggtctccggg gacgggtacg ccgccgccgt
      301 gctggacgcc ctgcggagga ggggatttgc agggtccctc ttcggcatgg gaggacccct
      361 cgccgccggt gcgggagcgg agatcgtctc cgactccagg gcccttcacc tcatgggcgt
      421 cacggatgtc cttcccgcca tcccccgcct tctccggctg aagaaaaacc tcgttgacct
      481 catcctctcc cggcggcccg gagcggtgct cctcatcgac agcccggact tccaccttcc
      541 cctcgccgcc tcgctccgcg ggagcggatg gcggggacca atcgtctacg cggcgccccc
      601 cacggtgtgg gcctggcgtc cgtggagggt gaagaacctc cgacgggaca tggatctctg
      661 tcttccgctc tttcagttcg agcacgagta cctcctccgg gccggggtca attcggcatg
      721 gaaaggacac cctctggtgg acgaattttc cgctcccctc ccggaggagg ctgatccgaa
      781 gcggatcgcc ctcctcccgg ggagcaggcg gtccgaagtg ttccgcctcc tgccccccct
      841 ggtggaatgc gcctcccttt tgaaggacag gggataccgc ccggtgttct ccatcgctcc
      901 cggaatgccc gaagatctca ggaaccgcct ccggaacgta ctgaaaggcc atgaaacctg
      961 ggaaggcccc gggcgggatt tgctggcatc gagcgctgcc gcggcggggg cgagcggcac
     1021 cgtggccgtt gaggccatga tgctcggcag gttcatggcc gttctctaca ggggaggctt
     1081 tctctcctgg ctcgcctgga agaccctggt gaagacgccc cgcatttcca tccccaacct
     1141 ccttgccgga gaggagctgt accccgagtt gatccagggc agggccacgg gcaggaatgc
     1201 cttttcggcc cttctgggct tcctggagga cgagcgggcc gcggagcata tccgggtagg
     1261 cctcctgtcg gcccggaggg ccatggggct cccgggagcc gtggacttct gggcggagcg
     1321 ccttgaagcc ctggtttcgg tgcaatcggc cttccagggg gaaggccggg acccatgaag
     1381 tccgttcttc ttcttgccaa cggaccggga gagctctggg gctgggtccg gcccgtggcg
     1441 gcggaactga aacgccgttc ccggaaagta tccctccttc tccttccctg ccagttcgcc
     1501 tccggcgagg agcgaagagt ggctgaaagc cttgcccctg acgaactccg gggaccttcg
     1561 ggccttgcgg gaacgttgag ggacgccctc tttcttggcc gcggcgccga ggcggtcctc
     1621 cagctcggag gtgacctggc ctgggggcgg attgccgccc gggctggacg ggcccctctt
     1681 ttgtgctatg cttacggcag aaaaaacggc cttccccgct gcgacagggt tttcacagca
     1741 tttcccgcca tggcggaagc catgggggac agggtatatg ttgccgggga cc
//
LOCUS       DAOTHD010000480         1791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_78143_length_1791_cov_3.879032, whole genome shotgun sequence.
ACCESSION   DAOTHD010000480 DAOTHD010000000
VERSION     DAOTHD010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78143_length_1791_cov_3.879032"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="P5219_08220"
     CDS             complement(<1..521)
                     /locus_tag="P5219_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRX26784.1"
                     /translation="MTLLCWAAGITLTVLVFSVLAFVFVRGYSVLSLDFLLEPPRDSM
                     TRGGIFTPLMGTLQLVAVAMAFAIPVGVATGLYFAEYARDDLFTRILRLAIRSLAGVP
                     SVVFGLFGLSLFVILFRFGPSLLSAALTLACLTLPLMVTASEQAFLAVPQDYRDGSYA
                     LGASKWQTIRRVVL"
     gene            complement(553..1416)
                     /gene="pstC"
                     /locus_tag="P5219_08225"
     CDS             complement(553..1416)
                     /gene="pstC"
                     /locus_tag="P5219_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRX26785.1"
                     /translation="MKDRLPGAIVKGISSAGILVMLFILYFLFREGLPVLRSVTLKEL
                     FLGDLWYPAENPPVLGMFPLIAATLAVTGLSCLLAVPFSLLIAVFVSETAPRPIREFL
                     KPILELLGFFPSIVLGFIGMVVLAPWLQETFDMLSGLNLFNASLLLGILTVPVVSSLA
                     EEALGAVPQDLRDASYALGATRWETTRKVVIPSALPGILSASLLGIMRAMGETMVVLM
                     AAGGAAIIPLSVFDPVRPLTSTIAAEMGETPVGSPHYAALFFGGVILLVMTLTVNLLS
                     AWIEKKGKMVR"
     gene            complement(1504..>1791)
                     /locus_tag="P5219_08230"
     CDS             complement(1504..>1791)
                     /locus_tag="P5219_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRX26786.1"
                     /translation="ASSGAMLTTVAKNKYAIGYDGIGYINDTVKPLMVNGKKGSAETA
                     LDGSYPIARFLYMFTKGWPTGEIMKFMNYMLGDEGQKVVVSTGFVPLRILK"
BASE COUNT          400 a          505 c          591 g          295 t
ORIGIN      
        1 agcaccaccc gccggatggt ctgccatttc gaggctccga gggcgtagga gccgtcacgg
       61 tagtcctggg gcacggcgag aaaggcctgt tccgatgcgg tgaccatgag gggcagagtc
      121 aggcacgcca gggtcagcgc ggcggaaaga aggctcgggc cgaaccggaa gaggatcacg
      181 aaaagagaga gcccgaagag gccgaagacc acagagggca ccccggcgag ggaccggatg
      241 gccagcctga ggatgcgggt gaacaggtcg tcccgggcat actcggcaaa gtagagcccc
      301 gtggccaccc cgacgggaat ggcgaacgcc atggccacag caacgagctg cagggttccc
      361 atgagggggg tgaagatgcc tcccctggtc atgctgtccc ggggaggttc caggaggaag
      421 tccagggaaa gaacggaata cccccgcacg aagacgaagg ccaggacgga aaaaaccagg
      481 acagtcaggg ttattccggc agcccagcag agaagggtca tgattttgtc cgctattcgt
      541 cggcgatcca tgctacctca ccatctttcc ctttttctcg atccaggccg agagaaggtt
      601 caccgtcagg gtcatgacca ggaggatcac gcccccgaag aagagagctg cgtagtgggg
      661 agaacccacc ggggtctccc ccatttcggc ggcgatggtg gaagtcagag gccggacggg
      721 gtcgaagacg gacaggggga tgatagccgc tccgcccgcg gccatgagga ccaccatagt
      781 ctctcccatg gcccgcatga tacccaggag agacgcgctc agtattcccg gaagggccga
      841 ggggatgacc actttccggg tggtttccca gcgggtggcc cccagggcgt aagaagcgtc
      901 ccggaggtcc tggggaacgg cgccgagggc ttcctcggca agagaactca ccacgggaac
      961 ggtgagaatg cccagcagga gggacgcgtt gaagaggttc agccccgaaa gcatatcgaa
     1021 ggtctcctgc agccatgggg cgaggactac catgccgata aagccgagga cgatggaagg
     1081 gaagaacccg agaagttcga gaatcggctt caggaattcc cggatcggcc ggggggccgt
     1141 ttccgaaaca aaaacggcga taagaaggct gaacgggacc gccagcaggc aagacagtcc
     1201 cgtcaccgcg agggtggccg cgatgagggg gaacatgccc agaaccggcg ggttttcggc
     1261 ggggtaccac aagtcgccga gaaacagctc tttcagcgtg acgctcctga gcacgggaag
     1321 cccttcccgg aaaaggaagt acaggatgaa gagcatgacc agtattcccg cgcttgaaat
     1381 tcccttcacg atggctcccg gcagcctgtc tttcatgaca atccctcttt catgattgaa
     1441 ttggtgctcc ctccccggga ctggggaggg agcaccggtt ccgctgtccg attcattcat
     1501 ccgctacttc agaatcctca ggggcacgaa ccccgtggag acaaccactt tctgtccttc
     1561 gtcgccgagc atgtagttca tgaacttcat gatctcgccg gtgggccatc ctttggtgaa
     1621 catgtacagg aagcgggcga tggggtagga tccgtcaagg gctgtctctg cggacccctt
     1681 ttttccgttc accatgaggg gcttcaccgt gtcgttgatg tagccgatgc cgtcgtagcc
     1741 gatggcgtac ttgttcttgg ccacagtggt gagcatggct cccgaggagg c
//
LOCUS       DAOTHD010000481         1790 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_78235_length_1790_cov_2.100865, whole genome shotgun sequence.
ACCESSION   DAOTHD010000481 DAOTHD010000000
VERSION     DAOTHD010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78235_length_1790_cov_2.100865"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="P5219_08235"
     CDS             complement(<1..958)
                     /locus_tag="P5219_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HRX26787.1"
                     /translation="MTDSRAVFFSGRLARLQEELGRIDADGLMVFSSESDNRPGIQYF
                     SGFTGSFAVLVLGRNGGRLVTDSRYFLQAEEESFFPLVRMEDRDPWPAVGQALRELDV
                     KSLAVEDDRLTLERGRALGKIASSVVGTSGLVRKLRGVKDEEELGIMRRSARIAAEAF
                     EAFLPAVRPGRTEAEIAADLVHEIRIRGAQQMAKGHFVTASGPRGARPHGVFSDRVLQ
                     LGDFITLDFGAVLDGYFSDMTRTVGLGDPDPKLVEIYHIVEEARKRAVAAVSSAATGR
                     DVDNAARDYIASKGWGAHFTHSTGHGIGLELHELPVVNRLNEE"
     gene            complement(1043..1618)
                     /locus_tag="P5219_08240"
     CDS             complement(1043..1618)
                     /locus_tag="P5219_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HRX26788.1"
                     /translation="MKDGLSSKEPDKKFAAVCGLYCEACSLYIATTEDPARLTALAKL
                     FQLPEEEMRCYGCRSEKRGPYCRTCEMAPCAAKQGIDFCGECAEYPCDILKAFQAERP
                     HRAELWEDLERIREAGYREWMAEKREHYSCPRCGTINSAYDLKCRKCGEEPGCAFTAM
                     HREKVEEALRLPLGGRKNSSGNSGKAEENQE"
BASE COUNT          314 a          594 c          495 g          387 t
ORIGIN      
        1 tctcctcgtt caggcggttc accacgggca gttcatgaag ctccaggccg atgccgtgcc
       61 ccgtggagtg ggtgaaatga gctccccagc ccttcgaggc gatgtagtcc ctggccgcgt
      121 tgtccacgtc ccggcccgtc gccgcggacg ataccgccgc cacagcccgc ttccgggcct
      181 cctcgacgat atggtagatc tccaccagtt tcggatcggg atcgcccaga cccactgtgc
      241 gggtcatgtc ggagaagtag ccgtcaagaa cggccccgaa atccagagtg atgaagtcgc
      301 ccagttggag cacccggtcc gaaaacacac cgtggggcct ggccccccgg ggacccgagg
      361 ccgtcacgaa atgccccttg gccatctgct gggctccccg tatgcgtatt tcgtgcacca
      421 ggtcggcggc gatctccgct tcggtgcgtc ccgggcggac ggccggaaga aacgcctcga
      481 aggcttcggc ggcgatccgg gcggaccggc gcattatgcc cagctcctcc tcgtccttca
      541 cccccctgag ttttcggacc agcccggacg tgcccaccac tgatgaggcg atcttcccga
      601 gggctcgccc ccgctccagg gtcagcctgt cgtcctccac ggcgagactt ttgacatcga
      661 gctcccggag ggcctgcccc acggcgggcc atgggtcccg gtcctccatc ctcaccaggg
      721 gaaagaagga ctcctcctcc gcctggagga aataccggga gtcggtaacg agcctgcccc
      781 cgttccggcc gagcacaaga acggcgaagg atccggtgaa gcccgaaaag tactggatgc
      841 cgggacggtt atccgactcg ctgctgaaga ccatcagtcc atcggcgtcg attcttccca
      901 gttcctcctg caggcgggca agacggccgg agaaaaagac ggccctgctg tcagtcattc
      961 tctcacctct cattgtgcca tagtccaaac agaatgtatt atactaaatc gatagtatct
     1021 tagggaagct tttttttcac ggctattcct gattttcttc agcttttccg gaatttccgc
     1081 tgctgttttt tcggccgccc aacgggagcc gaagggcttc ttccactttc tctctgtgca
     1141 tggcggtaaa ggcgcagccc ggttcctctc cgcacttccg gcacttcagg tcgtaggcgg
     1201 agttgattgt gccgcaccgg ggacaggaat agtgttccct tttttcagcc atccactccc
     1261 tgtaccccgc ctcgcggatt ctctccaggt cctcccagag ttcagcccgg tgggggcgct
     1321 ccgcctggaa ggctttcagg atgtcgcagg gatattccgc gcattcgccg caaaaatcta
     1381 tcccctgttt cgcggcgcac ggagccattt cacaggtccg gcagtagggt cccctcttct
     1441 ccgaacggca tccgtagcag cgcatctcct cctccgggag ctgaaatagc tttgccagcg
     1501 ccgtcagccg tgccgggtct tccgtcgtgg cgatgtagag ggagcacgcc tcgcaataca
     1561 gtccgcaaac ggcagcaaac ttcttgtccg gttctttgga gctcaatccg tccttcatgg
     1621 ttttcctccc tttgcatcca tgaaaccgtt tcggccttct tttccgttgc agctcttcag
     1681 atttccggca ggacagcctt ctaaaaaagt ttcacggagt ccgcaccacc gctttcccga
     1741 tcaccggaag aaggtcgtgg atggtctccg ccttttcgga aaaggcctcg
//
LOCUS       DAOTHD010000482         1783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_78626_length_1783_cov_3.115741, whole genome shotgun sequence.
ACCESSION   DAOTHD010000482 DAOTHD010000000
VERSION     DAOTHD010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78626_length_1783_cov_3.115741"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="P5219_08245"
     CDS             complement(<1..143)
                     /locus_tag="P5219_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HRX26789.1"
                     /translation="MTPTRSLVESSLLAGLAVILFLAAQFLPFVGAAFSLLAPAPLVI
                     LGLR"
     gene            complement(261..545)
                     /gene="rpsR"
                     /locus_tag="P5219_08250"
     CDS             complement(261..545)
                     /gene="rpsR"
                     /locus_tag="P5219_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRX26790.1"
                     /translation="MAVNEGRSEGRGPGGPGGRGGRRGGKRRPKVCFFCVDKIEHVDY
                     KDVEKLRKYVSERGKIMPRRVTGNCAKHQRQLTEAIKRARYMALLPFSVE"
     gene            complement(577..1065)
                     /gene="ssb"
                     /locus_tag="P5219_08255"
     CDS             complement(577..1065)
                     /gene="ssb"
                     /locus_tag="P5219_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRX26791.1"
                     /translation="MARGFNKVILMGNLARDPEIRYSVDKRAMARFAVAVNNTWKDRN
                     GELQESVDFIPVVVWGPSAENCERYLKKGSPVLVEGRIQVRSYEAKDGSGKRYATDVV
                     ASGVTFLGSKRQDDSSSGSFGDDEGYGDSVRKDRRFSSQDEDFPMDISEVRNEEDADI
                     PF"
     gene            complement(1078..1362)
                     /gene="rpsF"
                     /locus_tag="P5219_08260"
     CDS             complement(1078..1362)
                     /gene="rpsF"
                     /locus_tag="P5219_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRX26792.1"
                     /translation="MRPYEMMVLVSAEIEDPKEEIAKVEEVVRNLGGEVAKTDFWGKR
                     RLAYPIDKKTEGFYAVCNFSLNPDQLVELNRVLGLRPNIYRQMTVRLDEK"
     gene            complement(1442..>1783)
                     /locus_tag="P5219_08265"
     CDS             complement(1442..>1783)
                     /locus_tag="P5219_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26793.1"
                     /translation="SVSSAWQHRRGCNVLRLRWILLAAAVSLFAAIMGTAYFLEFRKI
                     GRLTALADERMAALVSMSRSVQELREKVAFYGTPDGVAHLAREQYNLSFPGEMVFKIE
                     VKKNSLPREKR"
BASE COUNT          350 a          572 c          477 g          384 t
ORIGIN      
        1 cgcaggccga gtatcacgag aggggctgga gcgagcagag aaaacgccgc cccgacaaag
       61 gggagaaact gggccgccag gaagagaatc acggcaagac ccgcgaggag ggacgattcc
      121 accaggcttc gtgtgggggt caccttttgg cctccttttt cgccgccctg aacccggaat
      181 gggttcgggg ggaacataaa aatttcggag gagaagctgg gctcctcccc cgaaaaaaga
      241 cgctgtttcg ctgcgaagcc ttactccacg gagaagggga gaagggccat gtatctcgcc
      301 cgcttgatgg cttccgtaag ctggcgctgg tgtttcgcgc agtttcccgt cacgcggcgg
      361 ggcatgattt tcccccgctc gctgacgtac ttgcggagct tttcaacatc tttgtaatcc
      421 acgtgctcta ttttgtccac gcagaagaaa cataccttcg gccggcgctt tccgcctctc
      481 cggccgccgc ggcctccggg gccgccgggt cctctgcctt cgcttctgcc ttcattcaca
      541 gccattgtgc actgccctcc ttaaaaaacc tctctgctag aaggggatgt ccgcatcctc
      601 ttcgttccgc acctcggaaa tatccatggg gaagtcctcg tcctgggagc tgaatcggcg
      661 gtccttccgg acgctgtctc catacccttc gtcatcgccg aaggagccgg atgacgaatc
      721 atcctgccgc ttcgagccga gaaacgtcac ccccgaggcg acgacatcgg tggcgtaccg
      781 ttttcctgag ccatccttcg cctcgtagct ccggacctgg atccggcctt ccacgagaac
      841 cgggctgccc ttcttgagat accgctcgca gttttcagcg gacggccccc agacgaccac
      901 ggggatgaaa tcgacgctct cctgcagttc accgttcctg tccttccagg tgttgttcac
      961 tgccacggca aaacgggcca tggcccgctt gtcgactgaa tagcgtattt cagggtcccg
     1021 ggcaaggttg cccatcagaa tgaccttgtt aaatcctctc gccacggcgc tgcctcctta
     1081 tttttcgtca agacggactg tcatctgacg atagatattc gggcgcagtc caagaacacg
     1141 gttgagctca acaagctggt ccgggttgag cgaaaaattg cacacggcgt agaagccttc
     1201 cgttttcttg tcgatgggat aggcaagacg ccttttcccc cagaaatcgg tcttggccac
     1261 ctctccgcca agattgcgca cgacctcctc gaccttcgcg atctcttctt tcggatcctc
     1321 gatctcggcg ctcacaagca ccatcatttc atatggccgc acgttccttc acctcctccc
     1381 tttggacttt tggccctctc ccgaggaaag ggcagggaaa aacttgcccc ggaattatac
     1441 cctatcgctt ctcccgaggc aaggaattct tcttcacctc gatcttgaac accatttctc
     1501 caggaaagga aagattgtac tgctcccgtg ccaggtgggc caccccatcg ggcgtgccgt
     1561 aaaaggccac cttttcccgg agctcctgga ccgaccggct catggagacg agagccgcca
     1621 tccgttcgtc cgccagcgcc gtgaggcggc ctatcttccg gaattcgagg aaataggccg
     1681 tccccatgat cgccgcgaag agcgacactg cggcagcgag gagtatccac ctgagccgga
     1741 gcacattaca tcctctccgg tgctgccacg ccgaggagac cga
//
LOCUS       DAOTHD010000483         1778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_78916_length_1778_cov_2.908880, whole genome shotgun sequence.
ACCESSION   DAOTHD010000483 DAOTHD010000000
VERSION     DAOTHD010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78916_length_1778_cov_2.908880"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="P5219_08270"
     CDS             <1..242
                     /locus_tag="P5219_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26794.1"
                     /translation="DLNSMHKIHSGTKSVNGELTPCYETVILSPLKRDRDRASGKRNV
                     ILSLHSTDDEERCFLEPKKKRRGHKETLSDAAVIF"
     gene            complement(597..1424)
                     /locus_tag="P5219_08275"
     CDS             complement(597..1424)
                     /locus_tag="P5219_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26795.1"
                     /translation="MKKLFGISLGLVLLFASLAGAAEFKQTAEFNVPEANQGIGVDEH
                     HFYAIDNRVVAKYDKATGKLVKKWEGPKDGPIIHLDSAAVIDGKIYCAHSNWSEWPMT
                     SSIEVWDAATLEHVANHSFGIHWGSATWVDRHDGYWWVAFANYDRPYGPNKTPYGHKI
                     CTTIVKFADNWQWLEAWTLPKAILERLEDMSNSGGSWGADGFLYLSGHDPAEVYKCRL
                     PKMGSVIELVEILPKNIRGQGIAWDRSETGVLYGIIRATKEEKEKGMTHKVIVSKLQ"
     gene            complement(1549..>1778)
                     /locus_tag="P5219_08280"
     CDS             complement(1549..>1778)
                     /locus_tag="P5219_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26796.1"
                     /translation="DGSPSPVGHGQGLDFWEIPSVSSDNDRIIVPGMRFNLHPSASFP
                     GGGKVSFCDCWISNEKSAERLSTLPCEIAYR"
BASE COUNT          365 a          495 c          477 g          441 t
ORIGIN      
        1 gtgacctgaa ttctatgcat aaaatacact caggaacgaa aagtgtcaac ggtgagctga
       61 cgccgtgcta cgagacagtg atattgtcac ccctgaaacg ggacagggat cgcgcctccg
      121 gaaagagaaa cgtcatactg tcacttcact ctacggatga tgaggagcga tgttttcttg
      181 agcctaaaaa gaagcgacga ggtcacaaag agacactctc tgatgcagcg gttatattct
      241 agttaggttc ccttttgtta atctcgaaaa agcattgaaa tacaaaattt tttttgattc
      301 gaaaattact ggtaggccgt gaaaggctca aatttcgggc attctagctt tttacattgc
      361 gccaggagta accccgtcat ctttaaggga atattaattc cctcaaaaaa acattggccg
      421 tttcgccctc cggaatcagt accatcgccc agtcaatgaa cccggcggcc tttccgcttg
      481 tttcggaaaa gccgccgggc agtttttgca tccatcctcc gtttcaaaat cgcggcacgg
      541 ccaaaggaag acgatttgta cccgccccaa atgcagcggg ttcctgtttc tctttattac
      601 tgcagcttgg agacgatgac cttgtgggtc atgccctttt ccttttcctc cttggtggcc
      661 ctgatgatgc cgtagagtac gcccgtttcg cttctgtccc acgcgatgcc ctggccgcgg
      721 atgttcttcg gcagtatttc caccagctcg atgacggaac ccattttggg aagcctgcat
      781 ttgtagactt ccgcagggtc gtggcccgac aggtagagga atccgtcggc tccccaggag
      841 cctcccgaat tgctcatatc ctccagccgt tcgagtattg ccttggggag ggtccacgct
      901 tcgagccact gccagttgtc ggcgaacttg actatggtgg tgcatatctt gtgcccgtag
      961 ggcgttttgt tggggccgta gggcctgtcg tagttggcga aggccaccca ccagtatccg
     1021 tcgtgccggt ccacccaggt ggcggacccc cagtgtatgc cgaagctgtg gttggccacg
     1081 tgctccaggg ttgcggcgtc ccacacctcg atggagctgg tcatgggcca ttcgctccag
     1141 ttggaatggg cgcagtagat ttttccgtcg atgacggcgg cgctgtcgag gtgaatgatg
     1201 ggcccgtcct tgggtccttc ccatttcttg acgagctttc cggtggcttt gtcgtacttg
     1261 gcgacgaccc ggttgtctat ggcatagaag tggtgttcat ccactccgat cccctggttc
     1321 gcttccggga cgttgaactc cgcggtctgc ttgaattcgg ccgctcccgc cagcgaagcg
     1381 aacagcaata caagccccaa agaaatccca aacagttttt tcattcgccg gtcctccttc
     1441 atacccgttc atttttcacc gattcccttt tcgtttgacc gtgccgcaca acacaaaccc
     1501 gcaccctgat gccggctttt ccggaggcag gcacactgga accgcctttc agcggtacgc
     1561 gatttcgcag gggagggttg acagacgttc ggcgcttttt tcgttggata tccagcaatc
     1621 gcagaaggag actttgcccc ctccggggaa agacgccgaa ggatggaggt tgaaccgcat
     1681 tccgggcacg atgatccggt cattgtccga gctgacggag gggatttccc agaaatcaag
     1741 cccctggccg tgccccacgg gagaggggga accgtctc
//
LOCUS       DAOTHD010000484         1774 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_79128_length_1774_cov_3.023851, whole genome shotgun sequence.
ACCESSION   DAOTHD010000484 DAOTHD010000000
VERSION     DAOTHD010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79128_length_1774_cov_3.023851"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="P5219_08285"
     CDS             complement(<1..203)
                     /locus_tag="P5219_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HRX26797.1"
                     /translation="MKIAVLIKQVPDSDDVRMDPETGTMVREGMGAIVNPLDLNALQA
                     ALDLRSAAGGEVTVITMGPPRAH"
     gene            complement(298..684)
                     /locus_tag="P5219_08290"
     CDS             complement(298..684)
                     /locus_tag="P5219_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26798.1"
                     /translation="MKGTVCVLAMILVFAGACFGLSFPDGQRQLGEAVLGSLTVKDGA
                     VVVNVASGGCTARESFKVHVEKSGGAEGDIPHYVLTFERIAADDCKAFLPEGVDLVFD
                     LEKDLGLSGRYTLSVANRVRPGKKPQ"
     gene            complement(735..1121)
                     /locus_tag="P5219_08295"
     CDS             complement(735..1121)
                     /locus_tag="P5219_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26799.1"
                     /translation="MKGEGAVAGKKIGLKPPAGMRTAVLAQTVLAVLFLPAGAFFAWI
                     AEGEAKPFALFFGLVWTAGCGFLFFRGRSALKALKEGTLALELDFEGEDSPGSRLREL
                     ESLRKDRFVSEEEYEKKRAEILEEKW"
     gene            complement(1276..>1774)
                     /locus_tag="P5219_08300"
     CDS             complement(1276..>1774)
                     /locus_tag="P5219_08300"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRX26800.1"
                     /translation="LALLLATNVALRLTNDLATVLSERGYSIAAEVVRGIIPIGSMHW
                     FDEIVEMCFAAMIFYGSASLWAVKGHFCVGDWISPRIRNFRAGSAYRLMIALFGAAFM
                     AVFFRYSLRLTLHSTELTTVFQIPKSLLYSCMPVSSFIMLVYSLADCLREGKALLRGS
                     GADPS"
BASE COUNT          368 a          584 c          522 g          300 t
ORIGIN      
        1 tcatgggccc ggggaggccc catggtgatc accgtgacct ccccgcccgc cgccgaccgg
       61 aggtcgaggg cggcctggag ggcattcagg tcgagagggt tgacgatggc ccccatccct
      121 tcccgcacca tggtccccgt ctcggggtcc atgcgcacgt cgtcggaatc gggaacctgc
      181 ttgatcagca ctgctatttt cacgtactaa aacctcctgt ccaaattcat ccgccggaaa
      241 gaacggcccc ttccgcagcc ggcggggagc ggtgccgccg tcatggaatc cgccggatta
      301 ttgcggtttt ttccccgggc ggaccctgtt tgccaccgag agggtgtacc gtcccgagag
      361 tccaagatcc ttctcaaggt cgaagaccag gtccacgccc tcggggagga aggccttgca
      421 gtcgtcggcg gcgatacgct cgaaggtgag gacatagtgg ggaatgtctc cctccgctcc
      481 cccggacttt tcgacatgga ccttgaagga ctccctggcc gtgcagcctc cggacgcaac
      541 gttcaccacc acggcgccgt ccttcaccgt gaggctcccg aggacggcct caccgagctg
      601 ccgctgcccg tcggggaacg aaagcccgaa gcatgctccc gcaaagacaa gaatcatagc
      661 cagcacacat accgtaccct tcatccttct tcctccttct cccctgccgg ccgctcccat
      721 cggacagttc cggctcacca tttttcctcc aggatctccg cccgcttttt ttcgtactcc
      781 tcttccgaga cgaaacggtc tttccggagc gattcgagct ccctcagccg tgaaccgggg
      841 gaatcctccc cctcgaaatc aagctccagg gcaagggttc cttccttcag cgccttcagg
      901 gcgcttctgc cccggaagaa gagaaaaccg caccctgcag tccagacaag gccgaaaaac
      961 agggcgaagg gcttcgcctc cccttcggca atccaggcaa aaaaggcgcc cgcgggcaga
     1021 aaaagaacag ccagaaccgt ctgggccagg acggcggttc tcatgcccgc aggaggcttc
     1081 agcccgatct tcttccccgc cacggcaccc tcccctttca tccgccaggc aactccgggg
     1141 cggaaaaaag ccccggtcat gacgaaacta tatcacaggg aagaaaaccc cgcattggag
     1201 tattttggag ggaaaacggg agaaaacaac agccctccgg agcggccgga acccgatccg
     1261 gagggccggg gcctgttatg acgggtcggc gccgctcccc cggaggaggg ccttcccctc
     1321 ccgcaggcag tcggcgagag aatagacaag catgatgaag gacgagacgg gcatgcagga
     1381 gtacagcagc gacttgggaa tctggaagac cgtggtcagt tccgttgaat ggagggtcag
     1441 cctcagggaa taccggaaga acacggccat gaaggctgcg ccgaacaggg cgatcatcag
     1501 gcggtaggcg cttcctgccc tgaaattccg gattctgggg gagatccagt cccccacgca
     1561 gaagtgcccc ttcaccgccc acagggaggc cgagccgtaa aagatcatgg cggcgaagca
     1621 catctccacg atctcgtcga accagtgcat ggatccgatg gggatgatcc cccggacgac
     1681 ctcggcggca atggaatacc ctctttccga gagcaccgtc gcgaggtcgt tggtgagtct
     1741 cagggccacg ttggtggcca ggagcagggc gagc
//
LOCUS       DAOTHD010000485         1772 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_79268_length_1772_cov_2.207338, whole genome shotgun sequence.
ACCESSION   DAOTHD010000485 DAOTHD010000000
VERSION     DAOTHD010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79268_length_1772_cov_2.207338"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            379..1017
                     /locus_tag="P5219_08305"
     CDS             379..1017
                     /locus_tag="P5219_08305"
                     /inference="COORDINATES: protein motif:HMM:NF024645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRX26801.1"
                     /translation="MKSCPGCGASLQEGASFCEYCGAQVRTEHTVPQSDPEKSPGAEN
                     SGRLHSREGGHSGPQVALRTVSPVFMLLLSFLTFLFYPVLWVFLRRKAFDSMVPEKKL
                     GLVLPLAFLAATVLFVAAGNIEKLPELASSLSFEEKESLTGWSFLAWFASMTMISFRM
                     RTILRKYASRTDGSPLAANLVARSSAMTFFFQFLYIQQHINMLVESGLAERE"
     gene            complement(1083..>1772)
                     /locus_tag="P5219_08310"
     CDS             complement(1083..>1772)
                     /locus_tag="P5219_08310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRX26802.1"
                     /translation="QEILAGNAALLEQHGVSVGGDPGTKPGAVVHVARDGRYAGLLVV
                     EDTLREEAREAVDRLRKLGVSTVGMLSGDRPENAGKFARELGLDLWEGGLLPAGKLDS
                     FRRIRNEAGGTTLFVGDGMNDAPLLAAADAGFAMGGLGADAAIEAADGVLLRDNPLGA
                     AEAIEIARRTRAIVWQNIAFALGVKGLVLVMGAFGVATMWEAVFADVGVALLATLNAA
                     RILQAAGSRNG"
BASE COUNT          334 a          592 c          487 g          359 t
ORIGIN      
        1 taatagacca cacccttgct caggcctgac cgctcgatga tcctgttgta ggaggccccg
       61 tcgataccgt tggcggcgat ttcctccatg gcggcctcga tgagggccgc ttttttttca
      121 tcatcaatct tcctggaact gtaacccaca gcgcacctcc ttacctttcc cgtacagacc
      181 gacaggttag accatccggt ctaatttgtc aacggggaaa ttcaggaagt ataatgcaga
      241 aggcgccctt ctcctgccga ctgcccggag aataattccg gcgcttccgg gacttggttt
      301 cccgcccggc ccgtttttcc tttttcctgt atccggggga aaggggccgc ctatttccat
      361 tgaaaaggag cgattttcat gaaaagctgc cccggatgcg gcgcatccct gcaggaaggc
      421 gcgtccttct gcgaatactg cggcgcccag gtaaggacag agcacaccgt tccccagtcg
      481 gatccggaaa aaagtccggg ggctgaaaac tccggaaggc ttcattccag ggaagggggc
      541 cattcgggcc cgcaggttgc cctgaggacg gtaagcccgg tcttcatgct cctcctctcc
      601 ttcctgacct tcctgtttta ccctgtcctc tgggtatttc tgaggagaaa ggccttcgac
      661 tccatggtgc ctgaaaagaa gctcggtctg gtccttcccc ttgcctttct tgcggccacg
      721 gttcttttcg ttgcggcggg aaacatcgaa aagcttcccg agctcgcctc atcgctgagc
      781 tttgaggaga aggaaagcct caccggctgg tcatttctcg cctggttcgc ttccatgacc
      841 atgatctcct tcagaatgcg gaccatcctg cggaaatacg cctcccggac cgacggcagc
      901 cccctggcgg cgaacctggt ggccaggtca tccgcaatga ccttcttctt tcagtttctc
      961 tacatccagc agcacataaa catgctcgtg gagtcaggcc tggcggagag ggaatgacat
     1021 ctgaaagagg aggcggcctg aaggccgccc cctctttcag atgccctcac gaaagggaaa
     1081 attcaaccgt tccgagaccc tgcagcctgc agtatcctcg ctgcgttcag cgtggcgagg
     1141 agggccaccc ccacgtcggc aaagaccgcc tcccacatgg tggcaacgcc gaaggctccc
     1201 atgaccagca cgagcccctt cacccccagg gcgaaggcga tattctgcca gacaatggcc
     1261 cgggtcctcc tggcgatctc aatggcttcc gccgccccga gggggttgtc ccggaggagc
     1321 accccgtcgg cggcctcgat ggcggcgtcc gcccccaggc cgcccatggc gaagccggcg
     1381 tcggccgcgg caagaagagg agcgtcgttc ataccgtcgc cgacgaagag ggttgttccc
     1441 ccagcctcgt tcctgatcct cctgaaggaa tcgagcttgc cggcgggaag gagtcctcct
     1501 tcccagagat caagaccaag ctcccgggca aactttccgg cgttctcggg acggtccccc
     1561 gacagcatac ccacggtgct cactcccagc ttccgcagac ggtccaccgc ctcccgtgcc
     1621 tcttcccgga gggtatcctc caccacgaga aggcccgcgt agcgaccgtc ccgggccacg
     1681 tggaccaccg ctccgggctt cgtaccggga tctcccccga cggaaacccc gtgctgctca
     1741 agcagagcag cgtttcccgc gagaatttcc tg
//
LOCUS       DAOTHD010000486         1771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_79305_length_1771_cov_3.039044, whole genome shotgun sequence.
ACCESSION   DAOTHD010000486 DAOTHD010000000
VERSION     DAOTHD010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79305_length_1771_cov_3.039044"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="P5219_08315"
     CDS             complement(<1..277)
                     /locus_tag="P5219_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HRX26803.1"
                     /translation="MKKTTLLRNMILERKALVCPGAHDAISAKLIERAGFKALQVSGF
                     GLSATYLGLPDMAFIGFSDVVHFTKNIVDAVKVPVMADADTGYGNSIN"
     gene            complement(303..>1771)
                     /locus_tag="P5219_08320"
     CDS             complement(303..>1771)
                     /locus_tag="P5219_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HRX26804.1"
                     /translation="MWKLKGINLEFVLSSLYYSPLGIFGSVTGMSATFISMFIIFGSL
                     LSATGGGKTFIDIALAITGKYTGGPAKAAVVSSALFGSISGSSVANVMVTGSYTIPLM
                     KRLGYRPEFAAAVEAIASSGGGITPPIMSISAFMMAEFLNISYFHIIGYALLPCLLYY
                     TGVFSGVHFRTVRMGIDKLPPEEIPRWKDILTLERMAGLIIPTAVLLYLIAIGQPLQL
                     AGFYACVSALAVFLLVGILKRKMKETMKLIVSALSEGGRDVAQIVPILVSVSMLVNLI
                     GLTGIAPKISGVILQIGGTNIYFSLLIATIVPFLLGTSLPVVPTYVLSVSILVPPLLK
                     LGIDPVAAHLFFIYWAILGGVTPPTCTAAVAAAGIAKANWVKTGFFSVRLGIVAFILP
                     YFFALNPALVGRAPWASIISHGITGFIGAISIAYGMFSHKECLLNPLRCISFVIGGML
                     LLFPGTTHSLIGLAVVGTAFVIDRKMLPDNGTAGSEKN"
BASE COUNT          418 a          453 c          511 g          389 t
ORIGIN      
        1 cgttgatgct gttgccgtac cctgtatcgg cgtcggccat cacgggaact tttaccgcat
       61 ccacgatatt tttcgtgaag tggaccacgt cgctgaagcc tatgaatgcc atgtccggaa
      121 gtccgaggta ggttgctgaa aggccgaatc cgctcacctg gagggccttg aatccggccc
      181 gctctatgag ttttgccgaa atggcgtcat gggctccggg gcacacaagt gcttttcttt
      241 cgagaatcat gtttctgagc agcgttgttt ttttcatttt ttttcccctc tcccgagtga
      301 attcagttct tctccgaccc ggcagtcccg ttgtccggga gcatttttct gtcgataacg
      361 aacgctgttc cgacgacggc aaggcctatg agcgaatggg tggttcccgg gaaaaggaga
      421 agcatgccgc cgatgacgaa cgagatgcat cgcagggggt tcagcaggca ttccttgtgg
      481 ctgaacatgc cgtacgctat ggagattgcc cctatgaacc ctgtaatgcc gtggctgatg
      541 atgcttgccc agggtgccct gccgacgagt gcgggattga gggcgaagaa ataggggagg
      601 atgaatgcaa cgattccgag ccgtaccgag aagaagcctg tcttgaccca gtttgccttc
      661 gcaattcctg ccgctgcgac ggccgcggtg catgtgggag gcgtcacacc tccgagtatg
      721 gcccagtaga tgaagaaaag atgtgctgcc acaggatcaa tgccaagctt gagaagggga
      781 ggcaccagga tggatacgga gagaacatat gtggggacca ccggaaggga cgtgccgaga
      841 aggaagggga caattgtggc gataagcagg gaaaagtaaa tgttcgtgcc gcctatctgc
      901 agaatgactc cgcttatctt cggtgcgata cccgtcaggc ctatgagatt gaccagcatg
      961 ctcaccgata caaggatagg aacgatctgg gcgacatccc tgcctccttc ggagagggcg
     1021 gatacgatga gcttcatggt ctccttcatc tttcttttca gaattcccac cagcagaaag
     1081 accgccaggg ccgagacgca tgcgtaaaac cctgccagct gaaggggctg ccctatggcg
     1141 ataaggtaca gcagaacagc tgtggggata ataagcccgg ccattctttc cagggtaagg
     1201 atgtctttcc accttggaat ctcctcggga ggaagcttgt cgatgcccat gcgcactgtc
     1261 cggaaatgga ctccgctgaa caccccggtg tagtacagca gacagggaag aagggcatac
     1321 ccgatgatgt ggaaatagga tatgttgaga aactccgcca tcataaaggc gctgatactc
     1381 ataatgggag gagtaatccc gccgccggat gaagcgatgg cttccaccgc agcggcaaat
     1441 tccggccggt agcccaaccg tttcatcaat ggaatggtat agctgcccgt gaccatgaca
     1501 tttgccaccg agcttcccga tatgctgccg aaaagtgcgc tcgagaccac agccgcttta
     1561 gcaggtccgc ctgtatattt gcctgtaatg gcaagggcta tgtcgatgaa ggtttttcct
     1621 ccccctgtag ctgacagcag agagccgaag atgatgaaca tggagatgaa cgttgccgac
     1681 atgcccgtga cgctgccgaa tattccgaga ggggagtaat aaagtgagct gagcacaaac
     1741 tcgaggttga ttcccttcaa tttccacatc c
//
LOCUS       DAOTHD010000487         1767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_79559_length_1767_cov_3.292056, whole genome shotgun sequence.
ACCESSION   DAOTHD010000487 DAOTHD010000000
VERSION     DAOTHD010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79559_length_1767_cov_3.292056"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="P5219_08325"
     CDS             complement(<1..263)
                     /locus_tag="P5219_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26805.1"
                     /translation="MANKKYKIALMGASGSGKTVFLGSYFNLITNLGQGRPVSFNTPE
                     AEDEAGRIIRTLFRQQIPVKEKARKNLFSYSVDSLGMDILFVEV"
     gene            717..>1767
                     /locus_tag="P5219_08330"
     CDS             717..>1767
                     /locus_tag="P5219_08330"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRX26806.1"
                     /translation="MYSEGFGTADRAEGIPADGKTRFNIGSTSKMFAAAAILILADEN
                     RLGLDDPVIRHLPEFSMKDPRYRDITIRMLFNHSSGLPGSTFVFGYRDDQNPHAVLLD
                     TLRESTLKHDPGAMGIYCNDGFTLAEMIVERVSGKPFLDFLKERVFEPLGMKDTGASI
                     GISGGKVAEFYDEKGKKLPRELVAVLGAGGLSSTVEDLCRFSGAFIPGGKSFLSEKMR
                     EEILRPQPTPFAASLRGQSLLEAFGWDYALLPEFRDLGFQMVGKSGGTSFYTTNIQIL
                     PSRGLAVAVIFSGHGPTQEATCTIMKALMRDKNLQVPEKPRPVPPAAAEPVPLELLAH
                     EGYYVDASRAAFFSFD"
BASE COUNT          401 a          440 c          467 g          459 t
ORIGIN      
        1 acttcgacaa aaaggatatc catgccgagc gaatcgacag aataggagaa gagatttttt
       61 ctggcctttt cttttacggg aatctgctgt ctgaaaagcg tccggataat gcggcccgct
      121 tcatcctccg cttccggggt attgaaagat acgggtctgc cctgaccgag gtttgtgatc
      181 aaattgaaat aggaacctaa aaagacggtt tttccggagc cggatgcgcc cataagtgca
      241 attttgtact ttttgttggc catatcctgt tccctccatg cagttgtata tgcattgcct
      301 tattccgaca tcagctgcaa ccataagagc aaaggaaaag tgtccggagg attcctcttc
      361 tcaccccaaa aagaatacca tagattgggt attatcagta actttttgac cctgcggctc
      421 ttccgttttt gtgtccggct tgtctcctgc gtacattggg ggtatattgc gaaaggtatg
      481 aaaagggtgg tgtcggcaac cggtttcaag tgtttttctg aaaggggtga aggttaatga
      541 aacgtgtgcg ttggccgctg tcagctgcaa tttttcttct cctgcttttg gtttcttttc
      601 ctgcggcagc ttcttatgat acggcaatcc ggacggcaag agaatttttg tggaaatcag
      661 tcacgtccgg gggcatcaac agttgttcgg cagcgatttt ggatggcggg gaaatagtgt
      721 attcggaggg cttcggcacc gccgacaggg cggaaggtat tcctgccgac gggaaaacca
      781 gattcaacat cgggtctacg agcaaaatgt tcgccgcggc cgcaattctt atccttgccg
      841 atgaaaacag gctgggactg gacgatcccg tgatacgcca cctgccggaa ttttccatga
      901 aggatccgag atacagggac attaccatac ggatgctttt caatcattca tcgggacttc
      961 ccggttcaac tttcgttttc ggatacaggg acgaccagaa tcctcacgct gttctgcttg
     1021 ataccctccg ggagagcacc ctgaaacacg accctggcgc aatgggaatc tactgcaacg
     1081 acgggttcac cctggctgaa atgatagtgg aacgggtttc cggaaagccg ttcctcgatt
     1141 ttctgaaaga aagggttttt gagcccctcg gcatgaaaga tactggtgcg agcatcggaa
     1201 tttcaggagg aaaagttgcg gaattttacg atgagaaggg gaaaaaatta cctcgggagc
     1261 ttgttgccgt cctcggcgca ggaggtcttt catcgaccgt tgaggatctg tgccgtttct
     1321 cgggtgcctt tatccccggc gggaaatcct tcctttccga aaaaatgcgg gaagagattc
     1381 tccgccccca gccgacgccc tttgctgcat cactgagggg tcagtcgctt cttgaagcat
     1441 tcggctggga ttatgccctt cttcccgagt tccgggacct gggtttccag atggtcggca
     1501 aaagcggagg gacttcattc tacaccacca atatacagat ccttccttcc cggggattgg
     1561 cagtggctgt tattttttcc ggtcatggac caacccagga ggcgacctgc actataatga
     1621 aagccctgat gagggacaaa aatctccagg tgcccgaaaa accccgtccg gtgcctcctg
     1681 cggcggctga acccgttcct ctggaactcc tcgcccatga gggctattac gtagacgctt
     1741 cacgggcagc ctttttctcc ttcgacg
//
LOCUS       DAOTHD010000488         1764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_79774_length_1764_cov_2.923347, whole genome shotgun sequence.
ACCESSION   DAOTHD010000488 DAOTHD010000000
VERSION     DAOTHD010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79774_length_1764_cov_2.923347"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="P5219_08335"
     CDS             <1..216
                     /locus_tag="P5219_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26807.1"
                     /translation="YIANRAARRAVPGSILLFHSGVKATIDALPEIVARLRKNGFEFV
                     TLDEMFSAYTASDPFLLRAAEGLFHTM"
     gene            230..1255
                     /locus_tag="P5219_08340"
     CDS             230..1255
                     /locus_tag="P5219_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HRX26808.1"
                     /translation="MGEFLVISTGGTIASRPGSHGLTPSLPGEELLSGVREMDRFGRI
                     TVLDLLSKDSSNMSPEDWKMMAACLRAEESEYDGFLILHGTDTMAYSASALSFFLPGF
                     SKPVVITGSMLPMGDPGSDAESNILEGFVFLRALAAQGRKGISIAFHGHLIHGPRSQK
                     ILSHDSTAFSSINYHELGCITSGNAVLGKEPFLAETYPVDVSSLSVENSIFLVTLFPG
                     FRARYLEHLTETQPRAIVLEALGLGGVPYLGENLLPPIARCREKNIPVIITTQCVYGG
                     VDLSVYEVGRKTLELGAISGKDMTREAIIAKLMITLPTTEPDRLEALLHKNFCDEITP
                     ERNLSQG"
     gene            complement(1246..>1764)
                     /locus_tag="P5219_08345"
     CDS             complement(1246..>1764)
                     /locus_tag="P5219_08345"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX26809.1"
                     /translation="KAYNLNKEPGKAFSSARQALDLMPGFPPALLELAHASRMVGDHR
                     EAVRLYEMYLAEEPKSVEALAGNSESLARLERWDESFASAMAAIREAPKKAEGYGALG
                     RAYRIAGRFEEAVEILRQGLVFRNGSTEILYDLGLSFIELGDRPSALAQYERLLELDP
                     ETASLLFRTIYP"
BASE COUNT          377 a          505 c          482 g          400 t
ORIGIN      
        1 tatatagcta acagggcagc ccgtagggca gtgccgggtt caatcctcct ttttcattcg
       61 ggagtgaagg caaccatcga cgctctccct gagattgtcg cccggctcag aaaaaacggg
      121 tttgaattcg tgacgctgga cgaaatgttt tccgcctaca cggcatccga tccattcctc
      181 ctgcgggcag cggaaggtct gttccatacc atgtaaggga gatgagttca tgggggagtt
      241 tctggttatt tcaacaggag ggacgattgc atcgcggccg ggaagccatg gcctgactcc
      301 gtcgcttccg ggagaggaac ttctctccgg agtcagggaa atggatcgtt tcggccgtat
      361 caccgttctt gatttgctct ccaaggacag ttcgaacatg tccccggagg actggaaaat
      421 gatggccgct tgtctcaggg ccgaggaaag cgaatatgac ggatttctca tccttcacgg
      481 tacggacacc atggcgtatt ccgcctcggc tctttccttc ttccttccgg gtttttcaaa
      541 accggttgtc ataaccggat ccatgcttcc catgggcgat ccgggatctg acgccgaaag
      601 caatatactt gagggtttcg tctttctccg tgccctggcg gcacagggac gaaaaggcat
      661 ctccatcgcc ttccacggac atctcattca cggaccccgg tcccagaaga tactcagcca
      721 tgactctacc gctttttcca gcatcaatta tcatgaactg ggctgcatca cgtcaggaaa
      781 tgcggttctg ggaaaagagc ccttccttgc ggaaacctat cccgtggatg tttcttccct
      841 ctccgtggaa aactccattt tcctcgtcac cctgttcccc ggtttccggg cacggtacct
      901 cgaacacctc acggaaaccc agccgcgggc catcgtactg gaggccctgg gactgggcgg
      961 agtcccctat cttggcgaaa atctcctccc gcctattgcg cggtgcaggg agaaaaacat
     1021 tccggtgatc ataacaaccc agtgtgttta cgggggagtg gacctgagcg tgtacgaagt
     1081 ggggagaaaa accctggaac tgggtgcaat ctcgggtaag gacatgaccc gggaggcaat
     1141 catcgcaaaa ctcatgatta ccctgccgac gacggaacct gaccggctgg aagcacttct
     1201 tcacaagaat ttctgcgacg agatcactcc cgaaaggaac ctctctcagg ggtaaatcgt
     1261 ccggaacagg agggatgccg tctccgggtc aagttcaagg agtctttcgt attgggccag
     1321 ggccgagggg cggtctccca gttcaataaa gctcagcccg agatcataca gaatctctgt
     1381 tgaaccgttc cggaaaacga gtccctgcct gagaatctct acagcctcct caaaacggcc
     1441 ggctatcctg tacgcccgtc ccagggcccc gtatccctcc gcttttttcg gcgcctctcg
     1501 gatagctgcc atggccgaag cgaaggactc gtcccagcgc tcgagccggg cgaggctttc
     1561 gctgtttcca gcgagtgctt cgaccgactt gggttcttcc gcgagataca tttcgtacag
     1621 cctgaccgct tctctgtgat cccctaccat acgggaggca tgggcgagct caaggagggc
     1681 gggaggaaaa ccgggcatca ggtccagggc ctgacgggcc gaagagaaag cttttccagg
     1741 ctctttgttg agattgtatg cctt
//
LOCUS       DAOTHD010000489         1759 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_80101_length_1759_cov_2.732394, whole genome shotgun sequence.
ACCESSION   DAOTHD010000489 DAOTHD010000000
VERSION     DAOTHD010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80101_length_1759_cov_2.732394"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..969)
                     /locus_tag="P5219_08350"
     CDS             complement(172..969)
                     /locus_tag="P5219_08350"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HRX26810.1"
                     /translation="MTPLKYRSIFLSDLHLGTRWCRAANISGFLSSVECDNLYLVGDI
                     IDGWNMGPGGSWPASHSSVLRSILSLAGKTSVTYIPGNHDEFMNRFDGQSLGGVRIRS
                     EAVHTGADGRRYLVVHGHQFDVVMLYSRWLAWAGDRAYSFTQHLNTGINFFRSLAGRE
                     YWSLSSFLKDRVKKAVKYFSRYESRIARSVREMDASGIICGHIHNPAVRAFQGVKYFN
                     CGDWVDHCSALVEHLDGVMEIIRWFDRKGESPAPRSAERVLGVPAAD"
     gene            complement(1043..>1759)
                     /locus_tag="P5219_08355"
     CDS             complement(1043..>1759)
                     /locus_tag="P5219_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26811.1"
                     /translation="GKADLTARASGTGGMVGMVFGSGQLSAKDGTVSGLDALKTVSDT
                     GAVKFSSVLASFNLDGRNIFLLPGSRVSAPVGDNVYRYFSASGSLGWNDSPLDLKCVG
                     DINVRALNAFLGALQGIIAVDGNPLTDPAFLQKFLSGLLGGMSVRDFRETSFNLRGTW
                     DAPVLTDLKVTSPAAPASIPRPNGSGKNETTIKITVEIPTGEGKDTSASTEDQVKKQL
                     LENIMKQIIRPGTDDGGSQE"
BASE COUNT          325 a          517 c          578 g          339 t
ORIGIN      
        1 cgtttccacc ttccgccggg ggaggaacgg cccgggatcg gaaggtggaa acgggcgaca
       61 gcaacggaat catggcggaa gtgctttcgg ggctcaaaga gggggaacgg gtgattcttc
      121 acccgtcgga gaaaatccgg gacggagtga aggtggtgca ggaatagggt gtcagtcagc
      181 cgccggaacg ccgaggactc tctccgcgga acggggagcg ggtgattcgc ccttccggtc
      241 gaaccaccgg atgatctcca tgacaccgtc gaggtgctcc accagggccg agcagtgatc
      301 cacccagtcg ccgcagttga agtactttac tccctggaag gcccggaccg ccgggttgtg
      361 gatgtgcccg cagatgattc ccgaagcgtc catttccctg accgaccgtg cgatgcggct
      421 ctcgtagcgg ctgaaatact tgaccgcctt tttcaccctg tccttgagaa aggaggaaag
      481 ggaccagtac tcccgtccgg cgagagaacg gaaaaagttg ataccagtgt tcagatgctg
      541 ggtgaagctg taggccctgt cgccggccca ggcaagccag cggctgtaga gcatcacgac
      601 gtcgaactgg tgcccgtgga ctacgaggta ccttctgccg tcggccccgg tgtggacagc
      661 ctcggaacgg atcctgacac ctccgaggga ctggccgtcg aaccggttca tgaactcgtc
      721 gtgattgccg gggatatagg tcactgaggt ttttcccgcg agagagagga tggaccggag
      781 taccgacgag tgggatgcag gccatgatcc tccgggcccc atgttccacc cgtcgatgat
      841 gtcccccacg aggtacagat tgtcgcactc tacggaggaa agaaagccgg agatgttggc
      901 ggccctgcac cagcgggttc cgaggtggag gtctgagaga aagatcgatc ggtatttcag
      961 cggggtcatc ccttcctttc cgaggcgcct ccgaggacgc cgcttcccat tatacagcaa
     1021 gggaaatgtc cggtctcgcc ggtcactcct gggagcctcc gtcgtcggtt cccggacgga
     1081 tgatctgctt catgatgttc tccaggagct gcttcttgac ctggtcctcc gtgctggcgg
     1141 aggtgtcctt gccttccccg gtggggattt ccacggtgat tttgatcgtg gtttcgtttt
     1201 tcccggagcc gttgggcctg ggtatggacg cgggggccgc cggggaggtc accttgaggt
     1261 cggtgagcac cggggcgtcc caggttcccc ggagattgaa ggacgtctcc cggaagtccc
     1321 ggacggacat gccccccagg aggcccgaga ggaacttctg gaggaatgcc gggtcggtga
     1381 ggggattccc gtccacggcg atgatgccct ggagggcgcc gagaaatgca ttcagggccc
     1441 ggacgttgat gtccccaacg cacttgaggt ccaggggaga atcgttccac cccagggacc
     1501 cgctggcgga gaagtaccta tagacgttgt cccccacggg agcggaaacc ctgcttcccg
     1561 gaaggaggaa gatatttctt ccgtcgaggt tgaagcttgc cagcacggag ctgaatttca
     1621 ccgctcccgt gtcggagacc gtcttcaggg cgtcgagccc cgacaccgtg ccgtccttgg
     1681 cggagagctg cccgctgccg aagaccattc ccaccatgcc cccggtgccg cttgccctcg
     1741 ccgtgaggtc cgcctttcc
//
LOCUS       DAOTHD010000490         1757 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_80212_length_1757_cov_3.230905, whole genome shotgun sequence.
ACCESSION   DAOTHD010000490 DAOTHD010000000
VERSION     DAOTHD010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80212_length_1757_cov_3.230905"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="P5219_08360"
     CDS             <1..210
                     /locus_tag="P5219_08360"
                     /inference="COORDINATES: protein motif:HMM:NF020253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HRX26812.1"
                     /translation="EHCRCLKDGREVGTVTSGGYCPSLDANMALCCVETPAPAEGESL
                     SVVIRDRPKQGVVVKRPFYDRKYKK"
     gene            250..648
                     /gene="gcvH"
                     /locus_tag="P5219_08365"
     CDS             250..648
                     /gene="gcvH"
                     /locus_tag="P5219_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRX26813.1"
                     /translation="MAKIPQDLRYTKDHEWIRMEGATGVMGITDYAQHAMGDIVYVEL
                     PDEGREVRAGEDFIVVESVKGANDVFSPVSGTVAGVNGDLDGAPELLNEDPYTNWLVR
                     LEVADPGELDSLLDAGAYAKLVEELEAESE"
     gene            702..1757
                     /gene="gcvPA"
                     /locus_tag="P5219_08370"
                     /pseudo
     CDS             702..1757
                     /gene="gcvPA"
                     /locus_tag="P5219_08370"
                     /inference="COORDINATES: protein motif:HMM:NF014408.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
BASE COUNT          375 a          571 c          551 g          260 t
ORIGIN      
        1 gagcactgtc ggtgcctcaa ggacggccgc gaggtgggca cggtgacctc cgggggatac
       61 tgcccctccc tggacgccaa catggccctg tgctgcgtgg agacccccgc gccggcggaa
      121 ggcgaaagcc tgagcgtcgt catccgggac aggccgaaac agggcgtggt ggtgaagcgc
      181 cccttctacg acaggaaata caaaaaatag gaacgacaat cccaggagag acaaaggagg
      241 acaaagaaaa tggcgaaaat accccaggat ctcaggtaca cgaaggatca cgaatggatc
      301 aggatggaag gcgccacggg cgtcatgggc attaccgact acgcccagca cgccatgggc
      361 gacatcgtct acgtggagct tcccgatgaa ggcagggagg tcagggccgg ggaggacttc
      421 atcgtcgtcg aatcggtgaa gggagccaac gacgtgttca gccccgtatc cgggacagtg
      481 gccggggtga acggcgacct cgacggagcc ccagagctgc tgaacgagga tccctacacc
      541 aactggctcg tccgcctcga ggtcgccgac ccgggagaac tcgactccct gcttgacgcc
      601 ggggcctatg ccaagctcgt cgaggagctt gaagcggaaa gcgaataggg gggaccgcca
      661 tgagccggta cattcccgcc acggcggaag accggcgccg tatgctggaa tacctcggcg
      721 tcggctccat tgacgacctt ttctcgggca tcccggagaa ggtcaggcag aacaaccttc
      781 ccgacctccc gaaggccctt ccggaggcgg agatgctccg ggacctcagg aaactggccg
      841 ggagcaacag gaacgccgac gacctgccct gcttcctcgg cgcgggcgtc tatgaccatt
      901 tcatccccgc cgccgtggac agcgtcgtct cccggggtga attcaccacg agctacaccc
      961 cctaccagcc ggaagtggcc cagggaactc tccaggccat cttccagtac cagaccatgg
     1021 tctgcgaact cacaggcatg gaggtctcca acgcctccat gtacgacggg cctcggccgc
     1081 cgccgaggcg gcggttctcg cggcatcggc gacgaagaaa agcaggattc tctggggacg
     1141 gaacgtgaac ccccagacga gggacgtgct ccgcacctac tgctggagca ggaagctcga
     1201 acacggggaa ctgcccgccg agaacgggcg gatcgacgca aaggccctgg agaacgccct
     1261 cgcagcgggc gacgcggcgg cccttgtggt ccagtccccc aatttcttcg gttctgtcga
     1321 agatctgaaa tccctggccg aaagggtcca cggggcggga gccctgctca tcgtctccac
     1381 ggacctcctc gccctcggcc tcctcgaagc tccgggaaaa ctcggcgccg atatcgtggt
     1441 gggcgacggg cagtccgtcg gcaacgccat gagcttcggc ggccccgcat tcggatttat
     1501 cgctgtcaac aagcccctca tgcgaaaaat gccgggccgc atctgcggcc agaccgtgga
     1561 ttccaaagga cggcggacct tcgtcctgac cctccaggcc cgggagcagc acatccgcag
     1621 ggagaaggcc acgtcgaaca tctgctcgaa ccagaacctc tgcatcgtcg ccgcgtcagt
     1681 ctatctcagc ctcatgggcc ccgccggact ccgggagatc ggcgaacaga tcatcgcaaa
     1741 aaccgagtac gcccttt
//
LOCUS       DAOTHD010000491         1751 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_80606_length_1751_cov_3.140330, whole genome shotgun sequence.
ACCESSION   DAOTHD010000491 DAOTHD010000000
VERSION     DAOTHD010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80606_length_1751_cov_3.140330"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /gene="argC"
                     /locus_tag="P5219_08375"
     CDS             <1..891
                     /gene="argC"
                     /locus_tag="P5219_08375"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRX26814.1"
                     /translation="GRFPELAFCSPEEGKAIAADIAFLALPHGASAPLIRECLDRGMK
                     VADLSGDVRLRNPADVEKWYGSAPLFPELLDTAVYGLPELYREKLKGADLASGVGCNA
                     SCSILGLYPLARAGLIGDVRIEVRVGSSEAGATPTAGSHHPFRSRALRVYEPFRHRHL
                     AEILQELNLSEERVTLTMTAVEMIRGVQMIAYVNLSRRVKEAELWKAYRSAYSGEPFV
                     SLCPARPSHLRLPEPKLVTGSNQALTGFTLHEDGTRLLVVTAIDNLMKGAAGSAVQSA
                     NLLLGLDETAGLGMLPVYPA"
     gene            912..>1751
                     /locus_tag="P5219_08380"
     CDS             912..>1751
                     /locus_tag="P5219_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridylate kinase"
                     /protein_id="HRX26815.1"
                     /translation="MNRRIQRGVVKIGGARGNAFGSLPEEIKERMERGEQWILAHGAS
                     SMMEDLSRAAGTEPLYVTSPGGFRSRFVSEGELALFEAACCRFSVRLAETLGKMGIHA
                     VPLYPPASKGAAAKRKDALRSVEDGKVRILRGNYSGSIVSFDPGDIHAVWEMGGLPLL
                     PPLAADESESGRILNVDGDRLAAAAAAAVGADVLAILSNVPGLLRDPGDPASKIDEGS
                     LDGWEDLERCARGNMKRKLLAAREALEGGAGAVVIADSRASSPVESGLSGGGTLLCRG
                     SMAA"
BASE COUNT          315 a          562 c          588 g          286 t
ORIGIN      
        1 ggacgttttc cggaactggc tttctgcagt cccgaagaag ggaaggcaat tgctgcggac
       61 atcgccttcc ttgctctccc ccacggggct tccgcccccc tgatccggga atgcctcgac
      121 agggggatga aggtggccga cctttccggg gacgtccgcc tcaggaatcc ggccgatgtt
      181 gaaaaatggt acggttcggc tccccttttt cccgaactgc tggacacggc ggtctacggt
      241 cttccggaac tctaccggga gaagctgaag ggagcggacc tggccagcgg cgtggggtgc
      301 aacgcctcct gctccattct cggcctgtat cccctcgccc gggccggtct gatcggcgac
      361 gtgaggatcg aggtccgggt gggatcctcc gaagcggggg cgacccccac cgccggaagc
      421 caccatcctt tccggagccg ggccctgagg gtgtacgaac ccttccgcca caggcacctt
      481 gcagagatcc tccaggagct gaacctctcc gaggaacgag tgaccctgac catgacggcg
      541 gtggagatga tccggggcgt ccagatgatc gcctatgtga acctctcccg cagggtgaag
      601 gaagcggaac tgtggaaggc ctacaggagc gcctattcgg gagagccctt tgtctccctc
      661 tgccccgcca ggccgtccca cctccgcctg cccgaaccga agctcgtcac gggcagcaac
      721 caggccctca ccggcttcac ccttcacgag gacggcacga ggctcctggt ggtaaccgcc
      781 atcgacaacc ttatgaaagg ggccgcggga agcgcggtcc agtcggcgaa ccttctgctg
      841 ggactggacg agactgccgg cctcggaatg ctgccggtct acccggcata acgactgcgg
      901 gaggaatgac catgaatcgc cggatacagc ggggcgtcgt caaaatagga ggggccaggg
      961 gaaatgcctt cggctccctc cccgaagaga taaaggaacg gatggagcgg ggcgaacagt
     1021 ggatcctcgc ccacggggcc agttccatga tggaggatct ttcccgggcc gccgggacgg
     1081 aacccctcta cgtcacaagc cccggaggtt tcagaagccg ttttgtgagc gagggcgagc
     1141 tggccctctt cgaagcagcc tgctgccggt tttccgtccg gcttgcggaa accctgggga
     1201 aaatgggaat ccatgccgtt cctctctacc cccccgcttc gaagggtgcg gcggcgaagc
     1261 ggaaggacgc cctccgctcc gtggaggacg gcaaggtccg catcctgcgg ggaaactaca
     1321 gcggctccat cgtgtccttt gaccccgggg acatccatgc cgtatgggaa atgggcggcc
     1381 tgcccctcct tcctcccctc gcggcggacg aatcggaaag cgggcggatt ctcaacgtgg
     1441 acggcgaccg cctcgccgcc gccgctgccg ccgccgtggg agcggatgtc ctggccatcc
     1501 tgagcaacgt gccgggactg ctccgggatc cgggggaccc ggcgtcgaaa atcgacgagg
     1561 ggagcctgga cggatgggag gacctggaac gctgcgccag gggaaacatg aagcggaagc
     1621 tcctggccgc cagggaagcc cttgaagggg gcgccggggc cgtcgtgata gccgacagcc
     1681 gggcgtcgtc gccggtggaa tcgggcctgt ccggaggagg aaccctgctg tgtcggggat
     1741 ctatggcagc c
//
LOCUS       DAOTHD010000492         1749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_80731_length_1749_cov_2.877214, whole genome shotgun sequence.
ACCESSION   DAOTHD010000492 DAOTHD010000000
VERSION     DAOTHD010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80731_length_1749_cov_2.877214"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..500
                     /locus_tag="P5219_08385"
     CDS             174..500
                     /locus_tag="P5219_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26816.1"
                     /translation="MKRRVFYTALLMVFLGSISAWGHPAWKGDLRKITEAGGVVYSLY
                     ADRTRLVEDCVPGAEQVAETYVHMVIPGQNLIEILQWNIRLNGKEYRVQDSFDYALDT
                     KGLVDQ"
     gene            625..1194
                     /locus_tag="P5219_08390"
     CDS             625..1194
                     /locus_tag="P5219_08390"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRX26817.1"
                     /translation="MEKEKLFYRMLDVIEGDIVPLTARGVAIGCKVFGAAVLRRDDLS
                     LVVAGTNHEAFSPLWHGEVYTIKLFYELQGHPDPSECLFLSTHEPCSMCISALAWAGF
                     RDIYYFFGYEQTQDDFNIPHDLRMLREVFGCVSPTKENMYYTSRSLLDIAQGLPEPEK
                     AEGRIAKIRETYAGLSAVYQAGEKKMVLK"
     gene            1421..>1749
                     /locus_tag="P5219_08395"
     CDS             1421..>1749
                     /locus_tag="P5219_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26818.1"
                     /translation="MKKILTAALAVLLAFSLSAPSFADSYVLESSGISFSLPDSWEAK
                     DSKMGTMFTPPAGDVMLVFGETTGEEAFDQAMEMVGDVAGEVVPDLELDDGTELDLNG
                     IKAIEFGG"
BASE COUNT          400 a          445 c          509 g          395 t
ORIGIN      
        1 gccgatgcac tggtcccggt tcacaacgtt caggtattgt accgccattc cattcacctc
       61 tctgtttgcg tctctctccg ccataatccc ctgtagattt acccctgtca actggtacaa
      121 taacatcgag tcgcacgaga accggtataa ccggaagaga aggagtgaag tacatgaaaa
      181 gacgggtgtt ttataccgcc cttctgatgg tgttcctcgg gagcatatct gcctggggac
      241 accctgcatg gaagggagac ctgcgaaaaa taacagaggc cggcggcgtg gtgtattctc
      301 tctacgcgga ccgtacaagg ctggtggaag actgcgttcc cggggcggag caggtggcgg
      361 agacctatgt ccacatggtg atcccgggcc agaacctgat cgagatactg caatggaaca
      421 ttcggctcaa cggaaaggag taccgtgtcc aggattcctt tgactatgcc ctggatacca
      481 agggactggt ggaccagtga acggtcttcc ccgagtgggc ctcgtggagg cccgttgaaa
      541 aaggatccgt ccaggagaag atcatcgcgg cggtgttcgg tatactgtct gaaaagaaat
      601 aatatgccga aaggggatga tggtgtggag aaggaaaagc tgttttatcg catgcttgat
      661 gtgatcgagg gggatattgt gcccctcacc gcccggggtg tcgccatagg ctgcaaggtt
      721 ttcggcgcgg cggtgctccg cagggacgat ctttcccttg tggtggcggg gacgaatcat
      781 gaagccttct cgcccctgtg gcacggcgag gtctatacca tcaagctgtt ctacgagctc
      841 cagggacatc ctgatccctc ggaatgcctt ttcctctcaa cccacgaacc ctgttccatg
      901 tgcatttccg ccctcgcctg ggcggggttc cgggacatct actacttttt cggctacgag
      961 cagacccagg acgatttcaa cattccccac gacctgcgga tgctccggga ggtcttcggc
     1021 tgtgtttcac cgacgaaaga gaatatgtac tacacgagcc gctcccttct tgacatagcc
     1081 caggggctcc cggagcctga aaaggcggag ggaaggattg cgaaaatacg ggagacctat
     1141 gcggggcttt ccgcggtgta ccaggccggc gagaaaaaaa tggtgctgaa gtaggatgat
     1201 gtgttccgac gccgcccttc ccagggcggc gtttttggtg tgcggtgccg gggacggaag
     1261 cgagggagaa aagagagaaa tgtaaaatta ttgtaaaggc tatttctttt ttgagtgaca
     1321 ggcagtatag tctataacat aataaaagaa gcaggattac tcgttccggg cttgaaaagc
     1381 cccccgctgt tcaaatccct ctacagaaag gatgaaacgc gtgaaaaaaa ttctcacggc
     1441 agcactcgcg gtacttctgg ccttttcact ttctgctccc tctttcgcgg attcctacgt
     1501 cctggaatca tcggggattt ctttttctct tccggattcc tgggaagcga aagattccaa
     1561 aatgggaaca atgttcaccc ctcctgccgg agacgtaatg ctcgttttcg gcgagacgac
     1621 gggagaagaa gcctttgatc aggccatgga aatggtgggg gacgtggccg gagaggtcgt
     1681 gcccgatctc gagctcgatg acggcaccga acttgacctg aacggcatca aggcgatcga
     1741 attcggcgg
//
LOCUS       DAOTHD010000493         1748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_80780_length_1748_cov_3.950975, whole genome shotgun sequence.
ACCESSION   DAOTHD010000493 DAOTHD010000000
VERSION     DAOTHD010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80780_length_1748_cov_3.950975"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="P5219_08400"
     CDS             complement(<1..255)
                     /locus_tag="P5219_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26819.1"
                     /translation="MTFSYFFYKTIGAFLTPPGIFVTVALFGALFFCPKSKKKGFPSF
                     SFAFFFALFLYALSIPLTARFLLLPLEEPYPLEIDGREGKA"
     gene            complement(252..929)
                     /locus_tag="P5219_08405"
     CDS             complement(252..929)
                     /locus_tag="P5219_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRX26820.1"
                     /translation="MSPAFNQGLRQIVYEKLREAIVNGAIRPGSKLSEIELAEQMAVS
                     RTPVREAIRQLAQTGLVTLTPRRGAFVTLPSLKDAADLYELRTELEILAVENLCAQPP
                     EEELLKFRKIFEEMTDATSAETYVREDRAFHLMIYQSCTNHFLTLMLNNISDLINLCR
                     PFSVERSPITHFTWGHLAIIDAVLSGDRPKAREETRAHITTSKDSLLEFLKNHRAEIS
                     REYHYGE"
     gene            complement(1023..1556)
                     /locus_tag="P5219_08410"
     CDS             complement(1023..1556)
                     /locus_tag="P5219_08410"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRX26821.1"
                     /translation="MGDVWITTKGGDKGETSIGNGERIPKDHPRVETYGTLDECQAHI
                     GMARSLCEYPEICECLCTLEQELGKLMGYIALYPGLSCPDIRMLEEFTGKVAEVTGKT
                     FRFVRPGDSTPGASLHVARTVARRAERAAVALFRTGELAEDAYRYINRLSDVIYALSL
                     WYDHLERAKKGDPTTCP"
BASE COUNT          388 a          468 c          484 g          408 t
ORIGIN      
        1 ggcctttcct tctctcccgt caatttccag ggggtacggt tcttccaggg gcagaagaag
       61 aaaacgggct gtcagcggaa tggagagggc atacagaaaa agggcgaaga aaaaagcgaa
      121 agaaaaggag ggaaaaccct tctttttgct tttggggcaa aaaaacaggg ccccgaaaag
      181 ggcgacggtc acaaaaatcc ccggaggagt cagaaaagct ccgattgtct tgtagaaaaa
      241 ataggaaaac gtcattctcc gtaatggtac tccctggaga tttcagcccg atggttcttc
      301 aggaattcca ggaggctgtc cttgcttgtc gtaatgtggg cccgggtttc ctcccgcgct
      361 ttcggcctgt ccccggaaag aacggcatcg atgatcgcaa ggtgtcccca ggtaaagtga
      421 gtgatggggg aacgctcaac ggaaaaaggc ctgcacaggt tgatcaggtc ggagatgtta
      481 ttcagcatca gggtaagaaa atggttggtg caggactggt aaatcatgag gtggaaggcc
      541 ctgtcctccc tgacatatgt ctctgcagag gttgcgtcgg tcatttcctc gaaaattttc
      601 ctgaatttca gaagctcttc ctccggcggt tgggcgcaga gattctcaac agcgagaatt
      661 tccagttccg ttctcagctc gtaaaggtca gccgcatcct tcaagcttgg aagggtaaca
      721 aaagcccccc gccttggcgt caaggtcaca agacctgtct gggcaagctg cctgatcgcc
      781 tcgcggacag gggtcctgga aacagccatc tgttcggcaa gctctatctc ggaaagcttc
      841 gaccccggcc gtatagcccc gttgacgatg gcttccctca gtttttcata tactatctgt
      901 ctcagcccct ggttgaatgc tggggacaag gaatttttct ccacaagctt tgacctcccc
      961 ttacagcaca atgtactcat tatactgcat gagaagtact tgtaaaagtg cctgaaacaa
     1021 ctttatggac aggtagtcgg gtcacccttt tttgcccgtt ccaggtggtc gtaccagagg
     1081 gagagagcat agatcacgtc ggagaggcgg ttgatgtacc tgtaggcgtc ctctgcaagc
     1141 tctcctgtcc ggaaaagggc cacggcggca cgttcagccc gtcgcgccac ggtccgtgct
     1201 acgtgcagag aagctccggg tgtggagtcg cccggccgga caaaccggaa agtctttccc
     1261 gtcacctcgg ccactttccc ggtgaattcc tccagcatgc ggatatcggg gcaggacagt
     1321 ccggggtaga gggcaatata ccccatgagc tttcccagtt cctgttcaag ggtacagagg
     1381 cattcgcata tttcagggta ttcgcacagc gaccgggcca ttccgatgtg tgcctggcat
     1441 tcatcaagag tcccgtaggt ttccactctc ggatggtcct tcggaattct ttcgccgttt
     1501 cctatggatg tttctccctt gtccccgcct tttgtggtga tccagacatc tcccatggca
     1561 gccgctccct tcttaatcct gtccgcagcc ggcgcagttt tcacagcttc ccccggcaca
     1621 tccgccgaga gtggagaaga ccacgcgaat aagggcgtct gtaagcactc cgggaggcag
     1681 agcctggtgt tccctgccct caatctccag ggttctcgtc ttgaagggtg tgctgtcggg
     1741 gagatggc
//
LOCUS       DAOTHD010000494         1745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_81004_length_1745_cov_2.921302, whole genome shotgun sequence.
ACCESSION   DAOTHD010000494 DAOTHD010000000
VERSION     DAOTHD010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81004_length_1745_cov_2.921302"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            471..>1745
                     /gene="aroA"
                     /locus_tag="P5219_08415"
     CDS             471..>1745
                     /gene="aroA"
                     /locus_tag="P5219_08415"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRX26822.1"
                     /translation="MKRILPAKKITGTVTLPGDKSISHRAALLGAVSVDGITAHNFSD
                     GADCASTLSCLEKAGADIHRKGTTLSVSAPSGLSSPSSVLDAGNSGTTARTLCGLLAG
                     KPGVRATITGDASLSKRPMLRIVKPLQSVGAAIDGPEGGASLPLEVTGKRLRGATHVL
                     ETASAQVKTALLLAGLSSDEPTTVIEPLASRDHTERMLAHLGIPLYIDGHSITMAPSK
                     PLRGGEWTIPGDFSSAAFWIVAAAVLPDSSISLPGVGLNPTRTGLLRVLERMGLAFSV
                     ERSGLWGGEPAGTITVHSSRLRATEVSGAEIPRLIDELPILAVAAALAEGTTEIRDAM
                     ELRFKESDRIVAVAKGLSALGAKVTELEDGWRITGPSRLSGGRVSASGDHRVAMALAV
                     AALAADSPVDIEDPECAAISYPGFFSTLDRHTG"
BASE COUNT          345 a          575 c          515 g          310 t
ORIGIN      
        1 accagcgaca tcgaacgggc accccggttc cgggacgctg ttccgggaag aggctctttc
       61 ggaaggaaag tatttattgc attaaaatat catttgtttt agatttgtat tttattaatg
      121 atattggata aagaatcccc ttgacaaggc ggacaaaaag ctgtatcttg cacgaaactt
      181 tcggggaaag gagaagcata ccgatgattt ccaggataaa cgcaaccttc gtgtttgaat
      241 tcgcgtttag ctgcgaccag ggatattatt attatcccct ggtctgttct gtactggaaa
      301 ccgatcgacg gcttgcttct caaagcttcg ggggataagg ggtcagcagc acaggcacaa
      361 cgaccgcccc ccgagggggg aaaagaaaga acagcaaaga ccggatccgg tttccagccg
      421 agatccggtc ttttttgttt tcaaaacaca cgaaaagggg cgtgcaggca atgaagagaa
      481 tccttccggc gaaaaagatt acgggaacgg tcacactccc gggcgacaag tccatatccc
      541 acagggcggc gctgctcggg gcggtgtccg tggacgggat tacggcgcac aatttctccg
      601 acggggcgga ctgcgcgtcc accctctcct gcctcgaaaa ggcgggagcg gacatccacc
      661 ggaagggaac gaccctctcc gtctccgctc cttcggggct gtcctctccc tcctcggtgc
      721 tggatgcggg caattcgggc acgacggccc ggaccctctg cggcctcctg gcgggaaaac
      781 ccggcgtcag ggccaccatt acgggggatg ccagtctgtc gaaacggccc atgctccgca
      841 tcgtgaagcc cctccagtcc gtgggagcag ccatcgacgg tcccgaaggc ggagcctccc
      901 tccccctgga agtgaccggg aagcgcctcc ggggagccac ccacgtcctc gaaacggcga
      961 gcgcccaggt caagacagcc cttctcctcg caggcctctc ctccgacgag cccaccaccg
     1021 tcatcgaacc cctcgcctcc agggaccaca cggagcggat gctcgcccac ctcggaatcc
     1081 ccctctacat cgacggacac tccatcacca tggcaccctc aaaacccctc agaggcgggg
     1141 aatggaccat ccccggcgac ttctcctccg ccgccttctg gatcgttgcg gcggccgtcc
     1201 tccccgattc gtccatctcc cttcccggcg tggggctgaa ccccacccgg acaggccttc
     1261 tccgggttct cgagcgcatg gggctggcct tctccgtgga gcggtcggga ctctggggtg
     1321 gcgagcccgc cggcaccatc accgtccatt ccagcagact ccgggcgacg gaagtttcag
     1381 gcgccgagat cccccggctc atcgacgaac tcccgatcct cgccgtggcg gcggcccttg
     1441 cggagggcac cacggagatc cgggacgcca tggagcttcg cttcaaggag agcgaccgga
     1501 tcgtggcggt ggccaaagga ctctcggccc tgggagcgaa ggtgaccgaa ctcgaggacg
     1561 gatggaggat cacagggccg tccaggctct cgggcggaag ggtctcagcc tccggcgacc
     1621 accgggttgc catggccctc gccgtggcgg ccctcgccgc cgacagcccc gtggacatcg
     1681 aggatccgga gtgtgcggcc atctcctacc cgggattctt ctccaccctc gaccggcaca
     1741 ccggg
//
LOCUS       DAOTHD010000495         1744 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_81055_length_1744_cov_2.798697, whole genome shotgun sequence.
ACCESSION   DAOTHD010000495 DAOTHD010000000
VERSION     DAOTHD010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81055_length_1744_cov_2.798697"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="P5219_08420"
     CDS             complement(<1..468)
                     /locus_tag="P5219_08420"
                     /inference="COORDINATES: protein motif:HMM:NF014766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26823.1"
                     /translation="MKVVRAETWLCRREASYFDRKRTVGAPMPWDVVVLRLSTDDGFS
                     STVGCLAARSGKVVEDYLHETILPVVLGRDVHDREAVWHEYWTVDRHLTFFPVYLPGP
                     VDVALWELAALRAGLPLYKYLGGYRKRLPVYASGLFHDGAEDYVREGTFYRDRG"
     gene            complement(542..1585)
                     /locus_tag="P5219_08425"
     CDS             complement(542..1585)
                     /locus_tag="P5219_08425"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRX26824.1"
                     /translation="MSGMKARTTMKDIARAAGVSSVTVHKAIYSKKGVSSETRDKILR
                     LAEAMDYSVNAAASSLKRGALHIAVVLQSVSNPVNYFFRSMWAGIDKVERALLDYRVR
                     LTRFECGDDWESQENILAGIAERKDIDGVVIQCWDETRLNGTINRLYDLGVPVVTANS
                     DAVGSKRIACVSGPNERIGRLAAELLRGFLPDGGKIVMMGGRPGVEIHNAHRRGFRAF
                     MEETGYSFPIVEVAGDGDPGRVRKDFSRALNGIGSGGVYAITSRDTLLACREIQERGL
                     ADRVRLVGSDVFRELVPFFEQGILRATVWKNQQAQAERAVLALYRYLTGRQAEWEPIG
                     IGVVLRNNLEDYL"
BASE COUNT          307 a          622 c          477 g          338 t
ORIGIN      
        1 tccccggtcc cggtagaatg tcccctccct gacgtaatcc tccgccccat cgtggaacag
       61 gccgctggca tacacaggca ggcgcttcct gtatcctccc aggtacttgt agaggggcag
      121 tcccgcccgg agagccgcca gttcccagag ggccacgtcc accgggcccg gcagatagac
      181 gggaaaaaac gtcaggtgcc ggtccaccgt ccagtattcg tgccagacgg cctcccggtc
      241 gtggacgtcc cgtccgagca ccacgggaag gatggtctcg tggaggtagt cctccaccac
      301 cttccccgac cgggcggcaa ggcaccccac cgtgctcgaa aacccgtcgt ccgtactcag
      361 gcggagcacc accacgtccc acggcatggg agcccccacc gttcttttcc tgtcgaaata
      421 cgaggcctcg cgcctgcaca gccatgtctc ggccctgacg accttcattt ccaccactcc
      481 cgatcccgtt atttttcgga aaaaatcggc gccggcctcc ttttctgaac tgccgccgaa
      541 cctacaggta atcctccagg ttgttccgga gcaccacccc gatgccgatg ggttcccatt
      601 ctgcctgccg ccccgtcagg taccggtaga gcgcgaggac ggcccgctcc gcctgggcct
      661 gctggttctt ccacaccgtc gcccggagga tgccctgctc aaaaaaggga acgagctccc
      721 ggaatacgtc gctgcccaca aggcgcaccc tgtccgcgag gccccgttcc tgtatttccc
      781 tgcaggcgag gagggtgtcc cgggaggtga tggcgtagac gccgccgctc ccgatcccgt
      841 tcagggcccg gctgaagtct ttcctcaccc gcccgggatc gccgtcgccg gccacttcca
      901 ctatgggaaa agaatatccg gtctcttcca tgaaggcccg gaatccccgc cgatgggcgt
      961 tgtgaatctc cactccgggc ctgcctccca tcatgacgat ctttccgccg tccggaagaa
     1021 accctcgcag cagttcggcg gcaagcctcc cgatcctttc gttggggccc gatacgcagg
     1081 cgatgcgttt ggatcccacg gcgtccgaat tcgccgtcac caccgggacg ccaaggtcgt
     1141 acaggcggtt gatggttccg ttcagccggg tctcgtccca gcactggatc accacgccgt
     1201 cgatatcctt ccgctccgca atgcccgcaa ggatgttctc ctggctttcc cagtcgtcgc
     1261 cgcactcgaa gcgggttagt ctcacccggt agtcgagaag ggcccgttca accttgtcta
     1321 ttcccgccca catgctgcgg aagaaataat tcaccgggtt ggagacgctc tgaagaacca
     1381 cggcaatatg cagagcacca cgcttgaggg acgacgcggc ggcgttcacc gaatagtcca
     1441 tggcctcggc gagcctcagg attttgtccc tcgtctcgct gctcacgccc tttttcgagt
     1501 aaatcgcctt gtggaccgtc acggaagaaa ctcccgccgc ccgggcgatg tccttcatgg
     1561 tcgtccgcgc cttcatgccg ctcattcagc cacgctcctc cgaatttcag atgtccgtcc
     1621 gacgacctct actccacggg cgtcaggctc tcacgcctga aggaaaaacc ccagcccggg
     1681 ccggtacggg gataggcgaa cccgttctcg atcctcatgg tgttgtcgat gatgccgtcg
     1741 atcc
//
LOCUS       DAOTHD010000496         1743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_81130_length_1743_cov_2.648104, whole genome shotgun sequence.
ACCESSION   DAOTHD010000496 DAOTHD010000000
VERSION     DAOTHD010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81130_length_1743_cov_2.648104"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="P5219_08430"
     CDS             <1..184
                     /locus_tag="P5219_08430"
                     /inference="COORDINATES: protein motif:HMM:NF024207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRX26825.1"
                     /translation="GLTIPELDPSICLGCGACEYACPAEPKAVTVSGLAVHGKIRPVS
                     GGEGGPTVAPLEEFPF"
     gene            209..400
                     /locus_tag="P5219_08435"
     CDS             209..400
                     /locus_tag="P5219_08435"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRX26826.1"
                     /translation="MNLGMGEILLLLALALVLFGAKRLPEVGRAVGSAVKEFKRGLET
                     GESPEKKENGEDGEPGQGK"
     gene            375..1112
                     /gene="tatC"
                     /locus_tag="P5219_08440"
     CDS             375..1112
                     /gene="tatC"
                     /locus_tag="P5219_08440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRX26827.1"
                     /translation="MGSPDRENSESWADHLEVLRGKIISVLAFFCISAVLLFSFAEVT
                     AAFLLAPLEGLGVSLYYFRPYEKLLTYMRLAFWGGMVLTAPLALVQGWLFIRPALRRN
                     EAKYLLIGGGIVPVLFAAGAAFAYRVIAPLALRFFLAFGEGDSILPLWGFGEYASFLF
                     SLLLASGLVFQAPLLLLALLLSGAVSLRTITRLRPWIILFIGLAAALLTPPDVVSQIL
                     LGVPLYLLFELTLAVARGVMGRRDDSG"
     gene            complement(1084..>1743)
                     /locus_tag="P5219_08445"
     CDS             complement(1084..>1743)
                     /locus_tag="P5219_08445"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRX26828.1"
                     /translation="GAVVWAQQTVPSSVAALIISIEPLWFVIFDWLFFKSRKPTLAES
                     VGLVLGFGGTVFLVLDQSGYSLSLSGEHTAGMLIVLVATVNWSMGALYSRKAPVAKSS
                     LLSTAMQMTAGGVLLLILSAAAGEPARLSLETISLKSVAALAYLVVFGSLIGFTAFVW
                     LLKVDRASRVVTHTFVNPLVALFLGWSIGGETVSVPMLVSAGIIIFSVVLITRSHPDG
                     P"
BASE COUNT          304 a          543 c          585 g          311 t
ORIGIN      
        1 gggcctgacc atccccgagc tggatccctc catctgtctc gggtgcgggg cctgcgaata
       61 cgcctgcccg gcggagccga aggccgtcac cgtgtcggga ctcgcggtgc acgggaagat
      121 ccgcccggta tccggcggag agggagggcc cacggtggcc cctctggaag agtttccatt
      181 ttgaccggca gagaacggag atgacggcat gaacctcgga atgggagaaa tacttctgct
      241 gctcgccctg gcgctggtgc tcttcggggc gaaacggctg cccgaagtgg ggcgggccgt
      301 gggaagcgcg gtgaaggagt tcaagagagg gctcgagacc ggcgaatctc cggagaagaa
      361 ggagaatgga gaggatgggg agcccggaca gggaaaatag cgaaagctgg gccgatcacc
      421 tggaggtgct ccggggcaag atcatttcgg tcctggcgtt tttctgcatc tccgccgtcc
      481 tgcttttctc cttcgccgag gttacggcgg cattcctgct cgcgccgctg gaggggctgg
      541 gcgtatcgct ctattatttc cggccctatg aaaaactgct cacctacatg cggctggcct
      601 tctggggagg aatggtgctc accgcacccc tcgccctggt ccagggctgg ctcttcatcc
      661 ggcccgcact gcgccggaac gaggcgaagt acctgctgat cggcggaggg atcgtgccgg
      721 tgctgttcgc cgcgggggca gcgttcgcct acagggtgat cgctcccctg gccctccggt
      781 tcttcctcgc cttcggggag ggagacagca tcctccccct ctggggattc ggagaatacg
      841 cttccttcct gttttccctc ctgctggcct cggggctggt gttccaggct cccctgcttc
      901 ttctcgccct cctgctttca ggagccgtct ccctgcggac catcacccgc ctcagaccct
      961 ggatcattct tttcatcggc ctggccgcgg ccctgctgac ccctcctgac gtggtgtccc
     1021 agatcctgct cggggtgccc ctctacctgc tgttcgagct gaccctcgct gtggcccggg
     1081 gtgtcatggg ccgtcgggat gactccgggt gatcagcacc acggaaaaga tgatgatgcc
     1141 ggcggaaacc agcatgggga cggaaacggt ctcgccgccg atgctccagc cgaggaaaag
     1201 ggccaccagg gggttcacga aggtgtgggt gaccaccctg gatgccctgt ccaccttcag
     1261 gagccagaca aaagcggtga acccgatgag ggacccgaag accacaaggt aggcaagggc
     1321 ggccacggac ttcagggaga tggtctccag ggagagccgg gccggttctc cggcggccgc
     1381 cgacaggatc aggagcagaa cccctcccgc ggtcatctgc atggccgtgg agagcaggct
     1441 cgatttcgcc acgggggcct tgcgggagta gagagccccc atgctccagt tcaccgtggc
     1501 gacgaggacg atgagcatcc ctgccgtgtg ctcccccgac agggaaagcg agtagccgga
     1561 ctggtcgagg acaaggaaga ccgttccgcc gaagccgagc accagtccga cgctctcggc
     1621 gagggtgggc ttcctggact tgaagaacag ccagtcgaag atgacgaacc agagaggctc
     1681 gatggagatg atgagggccg ccaccgacga tgggactgtc tgctgggccc acaccacggc
     1741 ccc
//
LOCUS       DAOTHD010000497         1743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_81143_length_1743_cov_2.364929, whole genome shotgun sequence.
ACCESSION   DAOTHD010000497 DAOTHD010000000
VERSION     DAOTHD010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81143_length_1743_cov_2.364929"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1722)
                     /gene="lpxK"
                     /locus_tag="P5219_08450"
     CDS             complement(<1..1722)
                     /gene="lpxK"
                     /locus_tag="P5219_08450"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRX26829.1"
                     /translation="MAVSKDRLRDIEALSDRNVKLIVADDAFQHRRMARDVDIALVDA
                     CCPFGNGWVAPAGILREPPAELSRASLVVITKSDQVPAEKLRTLADEIRRFVPEEKVF
                     FSRLSVPTWRRWNGCWSAERCEAPRAVLAFSAIGSPESFQRSLEQRGIAIVREHRFKD
                     HHRYGREDMEMLEEAFAGCGAEAMTCTEKDIYNLPTDWRPSRDILVPLISTELDDEPR
                     FRKALAEALRPRIVVASNGYGEDSMGALLARKLRERFPAAAVSSFPIVGKGEHYLKEG
                     IPVDSVPEDSPSGGVIKYRLSDLWRDLRSGLLRSIARQMGAWKRLRGAIRTPVCVGDV
                     YLLLHALWGQGQLPVLVATAKTVYLSGHWRLERFIIKHRSRIAWTRDRDTAEELLRSG
                     AVARFDGNPIMDLTCDNTIEKVSWGEKDGFRVLLLPGSRRRAYEDVRLLLDAVELLSE
                     RMACSYLMVIAPTLDMEELLASCRGWTSFEEDGRMGIRKGTASISFFFGPLPAVAEEA
                     HLLIGLGGTANQVCAGMGVPVVSIEEKGKFVQKKLLGESELLVPATARDLACGAEKIL
                     SDNELRLR"
BASE COUNT          301 a          570 c          579 g          293 t
ORIGIN      
        1 ccggaggcgg agttcattgt cggagagtat tttctcggcg ccgcaggcaa ggtcccgggc
       61 tgtggccggg acgagaagct ccgattcgcc gagcagcttt ttctgcacga acttcccctt
      121 ctcctctatg gacaccacgg ggacccccat gccggcgcag acctggttcg ccgtgccgcc
      181 caggcctatg agcaggtgag cctcctccgc gacggcggga aggggaccga agaagaagga
      241 gatggaagcc gttcctttcc gtattcccat ccgtccgtcc tcctcgaagg aggtccagcc
      301 ccggcaggag gccagcagct cctccatgtc gagggtgggg gcgatcacca tgaggtacga
      361 gcaggccatc cgttcactca gcagttccac ggcgtcgaga aggagccgga cgtcctcgta
      421 ggccctgcgg cggctgccgg gcaggagcag gacccggaat ccgtcctttt cgccccatga
      481 caccttctcg atagtattat cacatgtcag gtccattata ggattgccgt cgaaccgggc
      541 gacggcgccg gaccggagaa gttcctccgc ggtgtccctg tcccgggtcc aggcgatgcg
      601 gctcctgtgc ttgatgataa accgctcaag ccgccagtgg ccgctgaggt agaccgtctt
      661 ggcggtggcc acaaggacgg ggagctgtcc ctggccccac agggcgtgga gcaggaggta
      721 gacgtccccc acgcagacgg gggtgcggat ggccccccgg agccgcttcc acgcccccat
      781 ctgccgggcg atggagcgga gaaggcccga ccgaaggtcc ctccagaggt cggacaggcg
      841 gtacttgatg accccgccgc tgggggaatc ctcgggcacg gagtccacgg gaatgccttc
      901 tttgaggtaa tgctccccct tgcccacgat ggggaaggag gagacggccg cagcagggaa
      961 ccgctccctg agcttccgcg ccaggagggc tcccatggag tcttcaccgt agccgttgga
     1021 cgccaccacg atcctcggcc gcagagcttc cgccagggcc ttccggaagc ggggttcgtc
     1081 gtcgagctcc gtggagatga gggggacgag gatgtcccgg gaaggtctcc agtcagtggg
     1141 aaggttgtag atgtccttct cggtgcaggt catggcttcc gccccgcacc cggcgaaggc
     1201 ctcctccagc atttccatgt cctcccggcc gtaccggtgg tggtccttga accggtgctc
     1261 cctgacgatg gctattcccc gctgctccag ggatctctgg aaactctccg ggcttccgat
     1321 ggcggaaaag gcgaggacgg cgcggggggc ttcgcagcgc tctgcgctcc agcacccgtt
     1381 ccaccggcgc caggtgggaa ccgagagccg ggagaaaaag actttctcct ccggcacaaa
     1441 gcgccggatt tcatccgcca gggtccgcag cttttcggcc ggaacctggt ccgacttggt
     1501 gatcaccacc agggaggccc gggacagctc ggcgggaggc tccctgagga tgcccgcggg
     1561 agccacccaa ccgttcccga aggggcagca ggcgtccacc agggcgatat ccacatccct
     1621 ggccatccgc cggtgctgga aggcgtcatc ggcgacgatg agcttcacat tcctgtccga
     1681 gagggcttcg atgtcccgga gccggtcctt ggaaacagcc acgggaacgc cccgcagccg
     1741 gga
//
LOCUS       DAOTHD010000498         1739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_81372_length_1739_cov_2.944181, whole genome shotgun sequence.
ACCESSION   DAOTHD010000498 DAOTHD010000000
VERSION     DAOTHD010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81372_length_1739_cov_2.944181"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="P5219_08455"
     CDS             complement(<1..263)
                     /locus_tag="P5219_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HRX26830.1"
                     /translation="MKRLLAFLMLLLAEGSGDRRIPVGGQAVIEGVLMKGPARWGLSV
                     RKPDGMIESESWNNRSWTSSMPWKLPVIRGVVTMVEMMTVGFRA"
     gene            complement(271..972)
                     /gene="thyX"
                     /locus_tag="P5219_08460"
     CDS             complement(271..972)
                     /gene="thyX"
                     /locus_tag="P5219_08460"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HRX26831.1"
                     /translation="MACRVLLIASTPSPDAVVAAAARICYSDASAADLLEAESPEKSG
                     KLLHHLWKSGHYSPFEHASFTFAMDGLSRVASHQLVRHRIASFSQQSQRYVEMGEPDV
                     ILPPSVASNPDFSARFRAMAEEAHRFYAEMVEAGIPREDARFILPHGWSTRLVMTMNA
                     RELHHFFTLRLCRRAQWEIRDLARKMLRLVRRAAPLLFEKAGPSCVISGECREMNPCG
                     KPYASIEALLDEENS"
     gene            complement(1110..1346)
                     /gene="rpmE"
                     /locus_tag="P5219_08465"
     CDS             complement(1110..1346)
                     /gene="rpmE"
                     /locus_tag="P5219_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRX26832.1"
                     /translation="MKKDIHPRYEKCTVTCACGNTFETRSTDKEIRVGVCSSCHPFYT
                     GKKGSRVLTEAGRLEKFQRKYQGINYGQRETSDQ"
     gene            complement(1486..>1739)
                     /locus_tag="P5219_08470"
     CDS             complement(1486..>1739)
                     /locus_tag="P5219_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HutP family protein"
                     /protein_id="HRX26833.1"
                     /translation="NYVHLFLYLLRVVEKKRNEMHALMHAALEALDGFLVVGMLEASV
                     GAKIAIVRNSKWISVAVMGDTAYHAVAHHERCGLGVMHI"
BASE COUNT          389 a          520 c          458 g          372 t
ORIGIN      
        1 gcccggaaac ccacggtcat catctccacc atggtgacca cccccctgat gacgggaagc
       61 ttccacggca tggatgacgt ccaggaacgg ttgttccagc tttcgctctc gatcattccg
      121 tcaggcttcc tcaccgaaag cccccaacgg gcagggccct tcatcaggac accttcgatc
      181 accgcctgtc cgcctacggg tatccttctg tccccggatc cttccgcaag cagcagcatg
      241 agaaatgcaa gcagtctttt cattttcacc tcaggaattt tcttcgtcta aaagcgcttc
      301 aatggaagcg tagggtttcc cgcaggggtt catctccctg cattctccgg aaatgacgca
      361 tgacggacct gctttttcga aaagaagggg ggcggcccgc ctgaccagcc ggagcatctt
      421 ccttgccaga tcacggatct cccactgggc ccgcctgcac aggcgaaggg tgaaaaaatg
      481 gtgaagctcc ctggcattca tggtcatgac gagccgggtg ctccatccgt gggggagaat
      541 gaagcgggcg tcctccctgg gaatgccagc ctcaaccatt tcggcgtaaa agcgatgagc
      601 ctcctcagcc attgcccgga accgggccga aaagtccggg ttcgacgcga cggaaggggg
      661 aagaatgaca tcgggctcgc ccatctcaac gtatctctgg ctctgctggc tgaaggacgc
      721 gatcctgtgc cgaacgagct gatgggatgc caccctgctg agcccgtcca tggcaaaggt
      781 aaaggaggcg tgttcaaagg gtgaatagtg gccgcttttc cagaggtggt gcagcagttt
      841 cccgcttttt tcgggcgatt cggcctcgag aaggtcggcg gctgaggcgt cgctgtagca
      901 tatccttgcc gcagcggcta ccacggcgtc cggagacggc gttgaagcaa tcaaaagaac
      961 acggcatgcc atgaaatcgt tctccccttt ccgcttcgaa tccggcggcg aaggccgacc
     1021 cgaaatgaaa aaacagccag cgtcgcatca cgatgcggct ccggctgaaa gcggactctc
     1081 aaaaaaaggg gaccgcagcc ccctttcccc tactgatcgg aagtctctct ctggccgtag
     1141 ttgatgccct gatacttcct ctggaacttc tcgagccgtc ccgcctccgt cagcacacgg
     1201 ctgcctttct tgccggtgta gaaaggatgg caggaagaac agacgcccac cctgatctcc
     1261 ttgtcggtgg agcgggtttc aaacgtattg ccgcaggcgc acgtcaccgt gcatttctcg
     1321 tagcgagggt ggatatcctt tttcatctat gagacacctc cgaagaaaat cgtgacccgt
     1381 aaattgtcac caaacaaaca gaaactctat cacaggacgc ttccctgtgc aagagtcatt
     1441 tttcttcttc cgttatatga aaacagcgtc gaagcaggct taccgtcaaa tatgcatgac
     1501 cccgaggccg caccgttcgt gatgtgccac ggcatgatag gctgtatccc ccatgacggc
     1561 cacggaaatc cacttgctgt tcctgacgat ggcgattttt gcgccgacgc ttgcttcgag
     1621 cattcccacc acaaggaaac catcgagagc ttcaagagcc gcatgcatga gagcatgcat
     1681 ttcgttccgc ttcttttcca ccacacgcaa caaatacagg aataaatgta cgtaattgt
//
LOCUS       DAOTHD010000499         1733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_81814_length_1733_cov_2.687128, whole genome shotgun sequence.
ACCESSION   DAOTHD010000499 DAOTHD010000000
VERSION     DAOTHD010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81814_length_1733_cov_2.687128"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="P5219_08475"
     CDS             complement(<1..223)
                     /locus_tag="P5219_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HRX26834.1"
                     /translation="MAILDILKDYSELRDLSREDISALSGELRERITSVVLKNGGHLA
                     SSLGAVELILSLLRSFDPSEDRILFDVGHQ"
     gene            complement(225..1127)
                     /locus_tag="P5219_08480"
     CDS             complement(225..1127)
                     /locus_tag="P5219_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRX26835.1"
                     /translation="MNRGEFKLQYDRARVFIEESLEGLCSSPVSLVPPRLMESMAYSL
                     RAGGKRLRPVLCIQAGETFGLDREKTVPLALALEMIHTASLIHDDLPAMDNDTLRRGK
                     PTNHVLFGDALAILAGDALMAWAFEYPLAVLPDTGLPCDRICRSMHVLANALGPAGIC
                     GGQVLDTDGQSMDDSPDFPWKVTRQKTAVLLRASILSGAILAGADGKSLAALGAYGDH
                     LGSAFQIVDDILDVTSTAEELGKTPGKDESQNKRTFVAAYGLEKARELAARESRLASV
                     ALEGVRGDTAFFSDLSDYLCERTK"
     gene            complement(1124..1351)
                     /gene="xseB"
                     /locus_tag="P5219_08485"
     CDS             complement(1124..1351)
                     /gene="xseB"
                     /locus_tag="P5219_08485"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRX26836.1"
                     /translation="MKFSEKMAEIENIVGRLEKEALPLEEALSLFEKGVARIRECQAY
                     LREAKRRVTLLSSDGKEVPFSDGAANEEDGE"
     gene            complement(1348..>1733)
                     /locus_tag="P5219_08490"
     CDS             complement(1348..>1733)
                     /locus_tag="P5219_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26837.1"
                     /translation="GHDFAPLLRYISIRIRPNGRILAQRGAGSKCFSSGKRKNRGKNM
                     KAGKKDEFPKGKVPPSIPLFSLYCRRSLFLFSNERYTVNACMARSPKDGLKGVRLFFC
                     GHVVVYIFNRFYCIFSVILQGGASV"
BASE COUNT          382 a          500 c          460 g          391 t
ORIGIN      
        1 cctggtgccc cacatcgaag agaatccggt cttccgacgg gtcaaaggaa cgaagaaggg
       61 agaggatgag ttcaaccgct ccaagggaag atgcaagatg ccccccgttt ttcagaacga
      121 cggacgtgat ccgctcccgc agttcgccgg agagcgcact tatgtcttcc ctggaaaggt
      181 ccctgagttc gctgtagtct ttcaaaatat caagaatcgc catgtcactt cgtccgttcg
      241 cacagatagt ccgagagatc ggagaaaaag gccgtgtcgc cccgaacgcc ttcaagggcc
      301 accgaagcga gccggctctc gcgggcagca agctcccggg ctttttccag gccgtatgcg
      361 gcgacaaagg tccgcttgtt ctgcgactcg tcctttcccg gtgtttttcc cagctcttcc
      421 gccgtggacg tgacgtcgag gatatcatct actatctgga aggcggagcc gagatgatct
      481 ccgtaagcgc cgagggcagc cagagatttc ccgtcggctc cggcaaggat ggcccccgaa
      541 aggatggagg cccgaagaag gaccgcggtc ttctgccggg ttaccttcca ggggaaatcc
      601 ggtgaatcgt ccatgctctg cccgtcggta tcaagaacct gtccgccgca tattcccgcc
      661 ggacccaggg cattcgccag gacgtgcatg gatctgcaga tcctgtcaca gggcagcccc
      721 gtgtcgggaa ggacggcaag aggatactcg aaagcccagg ccatgagagc gtctcctgca
      781 agaatggcga gagcgtcacc gaaaagcaca tgattggtgg gctttcccct cctgagagtg
      841 tcattgtcca tggcaggcag gtcgtcatgt atcagcgacg cggtatggat catttcgaga
      901 gcgagagcga ggggcaccgt cttttccctg tcgagcccga aagtctctcc tgcttgtatg
      961 cacagaaccg gtctcaggcg cttccctccc gcccgaaggg aatacgccat ggattccatg
     1021 agcctcgggg gaaccaggga gaccgggctg gagcacaggc cttccaggct ttcttcgata
     1081 aaaaccctgg cccggtcata ttgaagcttg aattctcccc tgttcattcc ccgtcctcct
     1141 cgttcgccgc cccatcggaa aaaggcactt cctttccgtc ggatgaaaga agagtcaccc
     1201 gccgttttgc ctccctgagg taggcctggc attccctgat ccgggccact cccttttcaa
     1261 acagggacaa cgcttcttca aggggaaggg cttccttttc aagccgcccg acgatattct
     1321 cgatttcggc cattttttcg ctgaatttca tacgctcgct cctccctgaa ggattacaga
     1381 aaatatgcag taaaaccgat tgaaaatgta taccaccaca tgaccgcaaa aaaacaaccg
     1441 gacccctttc agtccgtctt ttggggaacg ggccatgcaa gcgtttacag tatatcgttc
     1501 atttgagaaa agaaaaaggc ttcttctgca gtatagggag aacaagggga tggagggggg
     1561 caccttccct ttcgggaact catccttttt cccggctttc atgtttttcc ctctgttttt
     1621 tctttttcct gaagaaaagc atttgctccc tgctcccctc tgtgctagaa ttcttccgtt
     1681 tggtcgaatc ctaatcgaaa tatatcgcaa caagggggcg aaatcatggc cag
//
LOCUS       DAOTHD010000500         1730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_81979_length_1730_cov_5.404179, whole genome shotgun sequence.
ACCESSION   DAOTHD010000500 DAOTHD010000000
VERSION     DAOTHD010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81979_length_1730_cov_5.404179"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="P5219_08495"
     CDS             complement(<1..544)
                     /locus_tag="P5219_08495"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRX26838.1"
                     /translation="MKERGGMFYGWWIVGATFTVLFIGLCSGFYTVSVFLEPIEQTFG
                     WNRTRISLGFTIAALLVGLLSPVVGIAVARLGARAVQLFGALVTGFGLILASFIQTLW
                     QYYIIYIVVAVGLASVSLVPSQTVISHWFEKRRGTAMGIIMTGIGLGGMAMVFIASMM
                     NEAYGWRWAYRFLGILVLAVV"
     gene            complement(676..1203)
                     /locus_tag="P5219_08500"
     CDS             complement(676..1203)
                     /locus_tag="P5219_08500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HRX26839.1"
                     /translation="MGLKGAAVQEITGRIWVFGDNIDTDVITPGAYVDAPLEEMTMHV
                     LESVCPDFPSRFEKGDIIVAGTNFGCGSSRETAPEALKAMGVGVIIAGSFARIFFRNA
                     VALGIPVLQVERSPLGFTQGDVIRVNIEEALVMNLTTGETIDGTPLYGDILDILRAGG
                     IIPLMKDIVEKRKRP"
     gene            complement(1194..>1730)
                     /locus_tag="P5219_08505"
     CDS             complement(1194..>1730)
                     /locus_tag="P5219_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HRX26840.1"
                     /translation="RGGFEPVYPDPDADYEKVLEVDINGLLPQVARPPRVDDVVPVDN
                     LDEISIDQAYIGSCTNGRLDDLAAAASILKGRKVHERVRLLVTPASYEVYKQAMARGI
                     LETIIDAGGCICHPGCGACFGGHMGILAPGETCISTTNRNFIGRMGSPDARIYLANPY
                     VVAASAVAGRIVPPETWA"
BASE COUNT          380 a          532 c          484 g          334 t
ORIGIN      
        1 ccacgacggc aagcacgagg attcccagga aacgataggc ccatcgccag ccgtaggcct
       61 cgttcatcat gctggcgatg aacaccatgg ccatgccccc cagcccgatg cctgtcatga
      121 tgatgcccat cgctgtgccc ctgcgcttct cgaaccagtg tgagatcacg gtctggctcg
      181 gcaccaggct cacgctggcc aggccgacgg ccacgacgat gtagatgatg tagtactgcc
      241 agagcgtctg gatgaagctt gccaggatca gtccgaatcc tgtcaccagg gcgccgaaga
      301 gctgcacggc cctggccccg agcctggcca ccgctatacc caccacggga ctcagcagtc
      361 ccaccaggag ggcggcaatg gtgaagccga gcgatatccg cgttctgttc cagccgaagg
      421 tctgctcgat aggctcgaga aagaccgaga ccgtgtagaa accagaacac aggccgataa
      481 acaacaccgt gaaggtggcg ccgacgatcc accacccgta gaacatgccc cctctttcct
      541 tcatggcaat cctccccgga taaaaaatgc aagtatttcc ttctgcaaag aataatcccg
      601 gggagaagga ttgcaaggaa ggtgctcatg gaagaaacga ggtgataggc atgaaacctt
      661 gaatcgaccg ggttattatg gtcttttcct tttctcgaca atgtccttca tcaaaggaat
      721 gatgcctccg gcccggagga tatccaggat atccccatac agaggtgttc cgtcgatggt
      781 ttctccggtg gtcaggttca ttacgagggc ttcttcaatg ttcacccgga tgacatcgcc
      841 ctgggtgaag cccagaggag agcgctccac ctgcagtacc gggataccca gagccacggc
      901 gttccggaag aagatgcggg caaaagatcc ggcgatgatg acgcccaccc ccatggcctt
      961 taatgcctcg ggggcggtct cccgtgaaga cccgcagccg aaattggttc cggccacgat
     1021 gatgtcgccc ttctcgaacc tggatgggaa atccgggcag acagattcca gtacgtgcat
     1081 ggtcatctct tccagaggag cgtccacgta ggccccgggt gtgatcacgt cggtatcaat
     1141 gttgtcgccg aagacccaga tgcggccggt gatttcctgc actgcagctc ctttcaggcc
     1201 catgtttcag gaggaacgat cctgcccgcc actgccgagg ccgccaccac gtacgggtta
     1261 gcaagataga tccttgcatc gggacttccc atacggccga tgaagttgcg gttggtggtt
     1321 gagatgcacg tctcgccggg cgccaggatt cccatgtgac ctccgaagca ggccccgcag
     1381 ccagggtggc aaatacaccc gccggcatcg atgatggtct cgaggatgcc ccgggccatg
     1441 gcctgtttgt agacctcata ggacgccggc gtcacgagga gcctcacccg ttcgtgcacc
     1501 ttcctcccct taaggatcga ggctgcagcg gccaggtcat ccaaccttcc gttggtacac
     1561 gacccgatat aggcctggtc gatggaaatc tcatccaggt tatccacagg caccacgtca
     1621 tccacacggg gagggcgggc tacctgtggc aggaggccgt ttatatccac ctcaagaact
     1681 ttttcatagt ccgcatccgg gtcaggatac accggctcaa agccccccct
//
LOCUS       DAOTHD010000501         1730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_81987_length_1730_cov_4.064478, whole genome shotgun sequence.
ACCESSION   DAOTHD010000501 DAOTHD010000000
VERSION     DAOTHD010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81987_length_1730_cov_4.064478"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="P5219_08510"
     CDS             <1..491
                     /locus_tag="P5219_08510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRX26841.1"
                     /translation="LDVGSGGGLPGLVWAICRPDLEVTLLDSVKKKCGALEEMAAALE
                     LPNVRVVWGRCEEYALRERETFSLAGARAVAGTGILLEYLAPFVAVGGTVLAMKGPLY
                     AEELEPLQGRWNRLGLAAPSIVPYGGDEKRRFLLLWKKNAPCSSRFPRKTGMAEKTFW
                     WR"
     gene            499..1269
                     /locus_tag="P5219_08515"
     CDS             499..1269
                     /locus_tag="P5219_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRX26842.1"
                     /translation="MLILTVSNQKGGVGKTTTCVNLAAELGRLGYSVLAVDIDPQANC
                     TSGLGISPEDFELSLYDVLLGEAEAKDAVLPTQWEGVSILPATIDLAGAEIELASVIS
                     RETRLRRHLRSLDSFDLAIVDCPPSLGMLTINALVASDKLIIPIQCEYYALEGVGQLS
                     KTVGLVRDSLNPELEISGVLLTMYDSRTRLSNEVAEEVRRQFGSVAFSSVIPRNVRLS
                     EAPSHAAPIGYYDPSSTGAQAYGDLAREVSGLWLKRAH"
     gene            1248..>1730
                     /locus_tag="P5219_08520"
     CDS             1248..>1730
                     /locus_tag="P5219_08520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRX26843.1"
                     /translation="MAKARALGKGLGALFTPPAESPLQPGGGQGEITSLPVDRIRPNP
                     AQPRRDMDEAALDSLAESIRNHGVVQPLIVRPLQEEGTYEIVAGERRWRAARSAGLTQ
                     VPVRVMEGSDRELREISLVENIQREDLSPLEIASAITELIQHHSLTQEEVAERIGWSR
                     T"
BASE COUNT          356 a          556 c          515 g          303 t
ORIGIN      
        1 tcctcgacgt gggctctgga ggcggacttc ccggcctggt ctgggccatc tgccgtcccg
       61 atctcgaagt gactctcctt gacagcgtga aaaagaaatg cggcgccttg gaggagatgg
      121 cagcggcgct ggagcttccc aatgtccggg tcgtctgggg aaggtgcgag gaatacgccc
      181 ttcgggaacg ggaaaccttc tccctcgcag gcgccagggc agtagccgga acgggaattc
      241 tcctcgaata tctcgccccc ttcgtcgccg tgggcggaac cgtactcgcc atgaagggcc
      301 ctctgtatgc cgaagaactc gagccgctcc agggacggtg gaacaggctc ggcctcgccg
      361 caccgtccat tgttccctac ggcggcgatg aaaaaaggag atttcttctt ctctggaaga
      421 aaaacgcccc ctgttcctcc cggttccccc ggaagacggg catggcggaa aaaacattct
      481 ggtggaggtg acgcccccat gctcattctc acggtttcaa accaaaaggg cggagtagga
      541 aaaacgacca cgtgcgtcaa tcttgcggct gaactgggcc gcctcggcta ttccgtcctg
      601 gctgtggaca tcgaccccca ggcgaactgc accagcggcc tgggcatatc gcccgaagac
      661 ttcgaactca gcctctacga cgtgcttctc ggcgaggccg aggccaagga cgccgtcctt
      721 cccacacagt gggaaggggt ctccattctt cccgccacca tcgaccttgc gggagcggaa
      781 atagaactcg ccagcgtcat cagcagggag acgaggctga ggcggcacct ccggtccctc
      841 gacagtttcg acttagccat cgtggactgc cctccctccc tcggcatgct caccatcaat
      901 gccctggtcg cctccgacaa gctcatcata cccatccagt gcgaatatta cgccctggaa
      961 ggagtcggac agctttcgaa aaccgttggt ctcgtccggg acagcctcaa tccggaactc
     1021 gaaatcagcg gagtgctcct gaccatgtac gattcgagaa ccaggctttc caacgaggtg
     1081 gccgaggagg ttcgccgcca gttcggcagc gtggccttct cctccgtcat tccgaggaac
     1141 gtcaggctgt ccgaagctcc gagccatgcg gcccccatag gttactacga cccctcctcc
     1201 accggggcgc aggcatacgg agatcttgcc agggaggtgt caggactgtg gctaaagcgc
     1261 gcgcactagg caagggactc ggtgccctct tcacaccacc ggctgaatct ccccttcagc
     1321 ccggaggcgg gcagggagaa atcacgtccc ttccggtaga cagaatccgt ccgaacccgg
     1381 cacaacccag acgggacatg gatgaagcgg ccctcgattc cctcgcggaa tccatacgga
     1441 atcacggagt cgtccagccg ctcatcgtca ggcccctcca ggaagaagga acctacgaga
     1501 tcgtggcggg cgagagacgg tggcgggcgg cccgctccgc cgggctcacg caggtaccgg
     1561 ttcgggtcat ggaaggaagt gaccgggaac tgcgggagat atccctggtg gagaatatcc
     1621 agagggaaga cctgtccccc ctggagattg cctcggccat tacggagctt atccagcacc
     1681 actccctcac ccaggaggaa gtggccgaac gcatcggctg gagcaggacc
//
LOCUS       DAOTHD010000502         1727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_82205_length_1727_cov_3.215311, whole genome shotgun sequence.
ACCESSION   DAOTHD010000502 DAOTHD010000000
VERSION     DAOTHD010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82205_length_1727_cov_3.215311"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..875)
                     /locus_tag="P5219_08525"
     CDS             complement(117..875)
                     /locus_tag="P5219_08525"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HRX26844.1"
                     /translation="MSGARVFERLAETFGKRLRNDCPYLLEEPLTISCLNVDQAIGNP
                     VPFTDFALQRGEERLVEAAFRGAKGQAFTSSPSSWQGTVADLLDLPPENDRNRAFFTA
                     GVNAVGRHLGWGEKTVHCRDASPKRCGETMGKHLAGELGPGERVALVGYQPAILGGLA
                     AALGSGRVCVLDLNPENIGRTVSGVTVLDGEKDLPAAASRSDLALATGSALTNGTIDS
                     ILEAFGGKRVIFFGTTIAVPAALLGFERLCFESE"
     gene            complement(872..1606)
                     /gene="modA"
                     /locus_tag="P5219_08530"
     CDS             complement(872..1606)
                     /gene="modA"
                     /locus_tag="P5219_08530"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRX26845.1"
                     /translation="MFKAARLFLLFLFLFCFSFPAFSSDDVYVAVSPGIAPCVEDIYS
                     LFAGAGGGPIVFVKEATGPLAQKMDAGAPFDLLVAADPEWPAWLAERGKLAGLQKCAD
                     GHLVLWAENKESCSEEKLPDLRIAAPDPETTSHGILAAGYLTSAGLWEEGLASKKILI
                     SRNALHAVLAVQAGSADAALIPESLAIGTKGAYIRLAVPPISTVAGLNTAGASETAGA
                     FFRFLLSPEAAGVWKKWGFEPAGAPK"
BASE COUNT          338 a          535 c          546 g          308 t
ORIGIN      
        1 gacaggatct cggcgatccg ggtgctggac gacctgggga actgacggtc cggtctcaca
       61 tgaagcggcg gcacggttcc gtgaagggac cgcgccgccg ttcagtttta ttccgttcac
      121 tcagactcga aacagagccg ctcgaaaccg aggagcgccg ccgggacggc gatggtggtc
      181 ccgaaaaaga tgacgcgctt ccccccgaag gcctccagga tgctgtcgat ggttccgttt
      241 gtcagcgccg acccggtcgc aagggccagg tcgctgcggg aggcggcggc aggaaggtct
      301 ttttccccgt cgaggacggt cactccgctg acggtccggc ctatgttctc cgggttcaga
      361 tccagaacac acacgcgacc gctgccaaga gccgccgcca gcccccccag gatggcgggc
      421 tggtagccga cgagggcaac cctttcgcct ggtcccagct ccccggcaag atgttttccc
      481 atcgtttcgc cgcatcgctt gggggaggca tccctgcagt gtactgtttt ttcgccccat
      541 ccgagatgtc tgccgacggc gttgaccccg gcggtgaaaa aggcgcggtt ccggtcgttt
      601 tccggcggca ggtccagaag atcggcgaca gtaccctgcc aggaagaggg ggaggatgtg
      661 aaggcctgcc ccttcgcccc ccggaaggcc gcctctacga gacgctcttc gccgcgctgg
      721 agggcgaagt cggtgaaggg tacaggattc cctatggcct ggtcaacgtt gaggcagctt
      781 atggtgagag gttcctccag aagatagggg cagtcattcc gcagacgctt tccgaacgtc
      841 tccgcaagac gttcgaacac ccgtgccccg ctcatttcgg agcccctgcg ggttcaaagc
      901 cccatttttt ccacactccg gcggcctcgg gggagagaag gaaacggaag aacgccccgg
      961 ccgtttccga cgctcccgcc gtattgagcc cggctacggt ggaaatcggc gggacggcaa
     1021 gccggatata ggcgcctttc gtcccgatgg ccaggctctc cggaatgagg gctgcatcgg
     1081 cgcttcccgc ctgaacggcc agaaccgcgt ggagggcgtt tctcgaaatg aggatcttct
     1141 tcgacgccag cccctcttcc cagagccccg ccgacgtgag atacccggcc gcgagaattc
     1201 cgtgggacgt ggtttccggg tccggggcgg ctatgcggag atcggggagt ttttcctcgg
     1261 aacaggactc tttgttttca gcccagagaa caagatggcc gtcggcgcat ttctgcagac
     1321 ctgccagttt cccccgttcc gcgagccagg cgggccattc cgggtcggcg gccaccagga
     1381 ggtcgaacgg tgcgccggca tccatcttct gcgccagggg gccggtggct tccttcacga
     1441 aaacgatggg gcctcccccg gctccggcga acaggctgta aatgtcctcg acgcagggcg
     1501 ctatgccggg ggatacggca acgtagacat catcggaaga gaaagcggga aaggagaagc
     1561 agaagagaaa caggaacagc agaaaaagac gggctgcctt gaacacggtg cacacacctc
     1621 catattttgc aacagagttg cattttttca agaataccac ccgccaaaac tatacgcaac
     1681 ccagttgcaa aacaacgcag tccgcgggag gatgaatctc cctgcgg
//
LOCUS       DAOTHD010000503         1716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_83003_length_1716_cov_2.452739, whole genome shotgun sequence.
ACCESSION   DAOTHD010000503 DAOTHD010000000
VERSION     DAOTHD010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83003_length_1716_cov_2.452739"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="P5219_08535"
     CDS             <1..373
                     /locus_tag="P5219_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HRX26846.1"
                     /translation="TPEKVLEIAKLPAETPRDVSIKKIEGVNDEILKEDLKTYFAANP
                     AQAPAPVKDVPANPDELKGSFTLDEVAAGYGLDGTEILKEAGWPADTPRSISLKEAGT
                     PLGREPSDIRTAVKELLKRKQ"
     gene            complement(416..1255)
                     /locus_tag="P5219_08540"
     CDS             complement(416..1255)
                     /locus_tag="P5219_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRX26847.1"
                     /translation="MFRNCIFSDPPVEDRSFPPSWSALTLRSEGCDLYGVMYHPAGKG
                     PHPTMVLFHGFPGTEKNGDLAQVFRRAGFNTIVFSYRGAWGSQGSFSFSHVVEDGRNV
                     LRFLRSPEARETFAVDRDRIIAAGHSMGGFAAVKAAEECPFVKDILFLSGWNVGLDGK
                     RASADPSLGQKLRGLLEECAPPLAGTDADALWNEIMDKREDFDLLNSAAAFAGRNVLL
                     VGASRDAITPPDVHHSSLLEALRKDGRAVVRDVMLDDDHGYSASRVALAEALLDHLEK
                     RGY"
     gene            complement(1263..>1716)
                     /locus_tag="P5219_08545"
     CDS             complement(1263..>1716)
                     /locus_tag="P5219_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRX26848.1"
                     /translation="QGKAIDHPDYRYKHRFLDRMKSLFEREYLVSEKIVWVGDMNVAP
                     TDMDVTSPEKKRDHPCFAPDIQEHFEDVKRWGFADLFRRYRPEPGEFSFFDYRVKNSL
                     EKNIGWRIDHVLVTPALEKLALDCWIDREPRSWEKPSDHTPVVAVFDL"
BASE COUNT          337 a          530 c          542 g          307 t
ORIGIN      
        1 gaccccggag aaggtgctgg aaatcgcgaa gctgcctgcc gaaactccca gggatgtgtc
       61 gatcaagaaa atcgagggcg tgaacgatga aatcctgaag gaagacctga agacctactt
      121 cgccgcaaac ccggcacagg ctcctgcccc ggtcaaagat gtgccggcga acccggacga
      181 actcaagggg agcttcaccc tggacgaagt ggctgcagga tacggcctgg acggcaccga
      241 gattctgaag gaggcgggct ggcccgccga taccccccgc agcatctctc tcaaggaggc
      301 gggaacgccc ctggggaggg agccttcgga catccggacg gcagtgaagg aactgctgaa
      361 gagaaaacag tagagtatcg aagggggctc ccggaggagc ccccttttcg tcctgtcagt
      421 accctctctt ctccaggtgg tccagtaacg cttccgccag ggccaccctg gatgctgaat
      481 atccgtggtc atcgtccaac atgacgtcgc ggacgacggc tctgccgtct ttccggaggg
      541 cctccagcag ggacgaatgg tgcacatcgg gcggggtgat ggcatccctc gaggcgccga
      601 cgaggaggac gttccggccc gcaaaggcag cggctgaatt gagaaggtcg aagtcctccc
      661 tcttgtccat tatctcgttc cacagggcat cggcatccgt tcccgccagg ggcggagcgc
      721 actcctccaa aagcccccgc agcttctgcc cgagggacgg gtcggcggag gcccgttttc
      781 cgtcaagccc cacgttccac cccgagagaa agagaatgtc cttcacgaaa ggacattcct
      841 ccgccgcctt gactgcggcg aagcccccca tgctgtgccc tgccgcgatg atgcggtccc
      901 tgtccacggc gaaggtttcc cgcgcctccg gactgcggag aaagcggagg acgttcctgc
      961 cgtcctcgac cacatgggag aaggagaagc tcccctggct gccccacgca ccccggtagg
     1021 agaaaacaat agtgttgaag cccgccctgc ggaacacctg ggcgagatcg ccgttcttct
     1081 ccgtgccggg gaagccgtgg aagagcacca tggtggggtg ggggcctttc cccgccgggt
     1141 ggtacatgac gccgtagagg tcgcacccct ccgagcggag cgtcagggcg ctccaggagg
     1201 gcggaaagga ccggtcttcc accgggggat ccgaaaagat gcagttcctg aacatggcgg
     1261 acctacaggt cgaacacggc caccacgggc gtgtggtccg agggcttctc ccagctcctg
     1321 ggctccctgt cgatccagca gtccagggcg agcttttcga gggccggcgt gacgagaacg
     1381 tggtcgatcc tccagcctat gtttttctcc agggagttct ttaccctgta gtcaaagaag
     1441 gagaactctc ccggttcggg tctgtacctg cggaacaggt cggcgaagcc ccagcgtttc
     1501 acgtcttcga agtgctcctg gatgtccggg gcaaagcagg gatggtcccg cttcttttcg
     1561 gggctggtta cgtccatgtc cgtgggagcc acgttcatgt ctcccaccca gacgatcttc
     1621 tcggacacga ggtactccct ctcgaaaaga gatttcatgc ggtcgaggaa acggtgcttg
     1681 taccggtaat ccgggtggtc gatggccttt ccctgg
//
LOCUS       DAOTHD010000504         1713 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_83178_length_1713_cov_3.307600, whole genome shotgun sequence.
ACCESSION   DAOTHD010000504 DAOTHD010000000
VERSION     DAOTHD010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83178_length_1713_cov_3.307600"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..549
                     /locus_tag="P5219_08550"
     CDS             187..549
                     /locus_tag="P5219_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HRX26849.1"
                     /translation="MNIRRFRKTIDAVLESLPPELFRQLNGGILVRPETKEDGDALIL
                     GEYVEDPDLGSLVLLYYGSFAEALEGASADEWEEEIEETVIHELRHHVESLAGVDYLA
                     REEEMELFQDADGEDEDD"
     gene            763..>1713
                     /locus_tag="P5219_08555"
     CDS             763..>1713
                     /locus_tag="P5219_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX26850.1"
                     /translation="MGAQILIVDDSSTDRMVIGNMLADFEILTACDGIEALQVIETHP
                     DLDLVILDLHMPNMDGFQLLEKLKAGKTPVKTPFLILTNYDELDKEIRGLKLGAEDYI
                     RKPVNLESLRVRVETHLELNRMQRLVEERLKKSTTLLQTILEQSPIGISLSFSKDPFR
                     PGEDLPPIINPALEKITGRTREDLLGLGWARITHPDDVEKNLEYYAALQAGEIKSYSM
                     EKRYIRPDGSVVWVDMTVAGLRLGDAGGNGHISLMQDITARKTAEIALRESERSKSVL
                     LSNLPGMAYRCRYDRDWTMEFVSDGCRELTGYGPDSLVGNR"
BASE COUNT          389 a          495 c          509 g          320 t
ORIGIN      
        1 atcatggccc tggccgatgt ctatgatgcc ctccgttcga accgaccgta caaggaggct
       61 ttcacccatg aacggagtgt cgggatcatc atcgaaggaa agggaagcca tttcgacccc
      121 gccgtggtgg acgccttcct ggcccgcgcg gcggaattcg ccgccatctg acaggaaggt
      181 ggaagcatga acatccgccg gttcaggaaa acgatcgacg ccgttctgga atctcttccg
      241 ccggaactgt tccggcagct caacgggggg atcctcgtcc gaccggaaac gaaggaggac
      301 ggggatgccc tcattctggg ggaatatgtc gaggaccccg atctcggcag cctggtcctg
      361 ctctattacg gcagttttgc cgaggcgctg gagggggctt ccgcggacga gtgggaggaa
      421 gagatcgagg aaacggtgat ccacgaacta cggcaccacg tggaatctct cgccggagtt
      481 gactacctgg cccgggagga ggaaatggag ctttttcagg acgctgacgg agaggatgag
      541 gatgactgat tttccgtgtg gaccggatgg ctgatttgca gtagactgac attgaggttg
      601 agggactttt ccttccgggg cagtccggct ttcggccgga cgaaggaaaa cgtttccctt
      661 gacgtgactt ttttctgaac cggtgtcccc ggcgcacaac gggcttcgct gtcgcttccc
      721 atgaatgaat atgcctggca gacgcacaaa ggggagatat caatgggcgc acagattctg
      781 atcgtcgacg actcatccac agaccgcatg gttatcggga acatgctggc cgattttgaa
      841 atcctcaccg cctgcgacgg catcgaggcc ctccaggtga tcgaaacgca ccccgatctc
      901 gatctcgtca tcctggacct ccacatgccc aacatggacg gcttccagct cctcgagaag
      961 ctgaaagccg gaaaaacccc cgtgaagact ccctttctta tcctgaccaa ttacgacgag
     1021 ctcgacaagg aaatccgggg cctgaaactg ggtgcggagg attacatccg gaaacccgtc
     1081 aaccttgaat ccctcagggt cagggtggaa acccatctcg aactgaacag aatgcagcgg
     1141 ctggtcgagg aacgactgaa gaaaagcacc accctgcttc agaccatcct cgaacagtct
     1201 cccatcggta tttccctctc cttcagcaag gatcccttcc gtcccgggga agaccttccc
     1261 cctatcatca accccgcgct tgaaaaaata acgggcagga ccagggagga cctcctcggc
     1321 ctcggatggg cccggataac ccatccggat gatgtggaaa aaaacctgga gtattacgcc
     1381 gcccttcaag ccggggagat caagagctat tccatggaaa agcggtacat cagaccggac
     1441 gggtccgtgg tctgggtgga tatgaccgtg gccgggctgc ggctcggcga tgctggcgga
     1501 aacggtcaca tcagcctgat gcaggacatc acggcccgga agacggccga gatagccctg
     1561 agggaaagcg agcggagcaa gtccgtcctc ctctccaacc tcccgggcat ggcctaccgg
     1621 tgcagatacg acagggactg gaccatggag ttcgtctcgg acggatgccg cgaacttacg
     1681 ggctatggcc ccgacagcct cgtcgggaac agg
//
LOCUS       DAOTHD010000505         1712 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_83252_length_1712_cov_3.346409, whole genome shotgun sequence.
ACCESSION   DAOTHD010000505 DAOTHD010000000
VERSION     DAOTHD010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83252_length_1712_cov_3.346409"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="P5219_08560"
     CDS             complement(<1..784)
                     /locus_tag="P5219_08560"
                     /inference="COORDINATES: protein motif:HMM:NF013112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRX26851.1"
                     /translation="MMNGLEGKKIWIYSLGCRSNQYEGEALANAFTDAGAELVDSPED
                     CDGAVLVSCTVTAMADKKCRQAVRRARRQSRNALVAVCGCWAQKITRTEAHSLGISIV
                     VGNRRKGELPALLAGAFREETPSFVEDRVDVLRSREWDGLFLSRPLLHTRAFVKIQDG
                     CNHFCSYCAIPYVRGFPVSRDPGDILSEVEQIAGSGCSEVVLTGVHLGLYGKYGSVSL
                     AEIVRRIASVEGLRRIRFGSLEPFGLDDELLEALAAEPRFCRH"
     gene            complement(762..1649)
                     /locus_tag="P5219_08565"
     CDS             complement(762..1649)
                     /locus_tag="P5219_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRX26852.1"
                     /translation="MTNTTDGFWWYVTLEGGPGSEEVLASLAEMSGSIGSEESAAGNA
                     VRVRAYFRASQDLGFWVERLEEALSPWPEIKIADMGKIENKQWHTDWKDAFPPLPVGE
                     RLVVLAPWHKGTEPEGRTPLYIYPGSAFGTGYHESTQIVLTLMERLSLDGAEGADIGT
                     GSGILAIAAVKMGAARVRARDLDPAVLSEVRHNLELNDIGPGSVELQEGNLLDGMAGP
                     VDFLTANIIIEPLLDMLPAVPGVLGEGGRALFSGLLAKERERFAAALGEQGLRIVDEL
                     VINDWWGVAAERNDERSRR"
BASE COUNT          318 a          562 c          506 g          326 t
ORIGIN      
        1 ggtgccggca gaaccggggc tcggccgcca gggcttcgag cagttcgtca tcgaggccga
       61 agggttccag ggatccgaag cgtatccgcc gcagcccttc cacggaggca atgcgccgca
      121 cgatttccgc cagggagacg gagccgtact ttccgtacag gccgaggtga acgccggtga
      181 gcaccacttc ggaacagccc gatcccgcga tttgttcaac ttcggagagg atgtcgccgg
      241 gatcccggct tacgggaaac ccccggacat aaggaatggc gcagtaggaa cagaaatggt
      301 tgcacccgtc ctggatcttc acgaaggccc gggtatgaag gagcggccgg ctcaggaaca
      361 gtccgtccca ttcccttgaa cggaggacat ccacccggtc ctccacgaag gagggggtct
      421 cctcccggaa agccccggcg agaagggcgg gaagttcgcc tttgcgccgg ttgccgacca
      481 cgatcgaaat cccgagggaa tgggcttctg tcctggtgat tttctgggcc cagcatccgc
      541 agacagccac cagggcgttc cgggactgcc gccgggccct gcggaccgcc tgtcggcatt
      601 ttttgtccgc catggcggtg acggtgcagc tcacaagcac cgccccgtcg cagtcctccg
      661 gcgaatccac aagttccgcg ccggcgtcgg taaaggcgtt tgcgagggct tcgccctcat
      721 actggttcga acggcagccc agggaataga tccagatttt cttaccttcg agaccgttca
      781 tcattccgtt cagcggcaac tccccaccag tcgttgatca ccagttcgtc cacgatccgc
      841 agcccctgtt cccccagggc ggcggcgaac cgttccctct ccttcgccag gagtcccgaa
      901 aagagagcac ggccgccctc ccccagaaca ccgggtaccg ccggaagcat gtcaagaagg
      961 ggttcgatga tgatgttcgc tgtaaggaaa tccaccggcc ccgccatgcc gtccaggagg
     1021 tttccttcct ggagctccac gctccccggg ccgatgtcgt tgagctccag gttgtgccgg
     1081 acttccgaca aaacggcggg gtccaggtcc ctggcccgca ccctcgcggc ccccattttg
     1141 acggccgcta tggcgaggat gcccgacccc gtgccgatgt cggctccctc ggctccatca
     1201 agggaaagtc tttccatgag ggtgaggacg atctgggtgc tttcatggta gcccgtcccg
     1261 aaggccgatc ccggatagat atacaggggg gttcttccct ccggctcggt gcctttgtgc
     1321 cacggggcga ggaccaccag cctctccccc accggaagcg gagggaaagc gtctttccag
     1381 tccgtgtgcc actgcttgtt ttctatcttt cccatgtcgg ctatttttat ctcgggccag
     1441 gggctgaggg cttcttcaag gcgctccacc cagaacccca ggtcctggga ggcccggaaa
     1501 taggccctca cgcggacggc gtttcccgcc gcactttcct ccgaaccgat gcttcccgac
     1561 atttcagcga gggatgcgag cacttcctca ctcccggggc ctccttccag ggtgacatac
     1621 caccagaaac cgtccgtcgt atttgtcatg gaaaatctcc tccggggata tgaagtaagg
     1681 gaggcttaag cctcccttaa acagaaaaag tg
//
LOCUS       DAOTHD010000506         1711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_83338_length_1711_cov_2.694444, whole genome shotgun sequence.
ACCESSION   DAOTHD010000506 DAOTHD010000000
VERSION     DAOTHD010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83338_length_1711_cov_2.694444"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="P5219_08570"
     CDS             complement(<1..209)
                     /locus_tag="P5219_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HRX26853.1"
                     /translation="MNNMLHVVVLALLVLSAFFALWFKDLISSVISLAAFSVMTALEF
                     YILQAPDVAIAEAAIGAGLSTTIYI"
     gene            complement(206..577)
                     /gene="mnhG"
                     /locus_tag="P5219_08575"
     CDS             complement(206..577)
                     /gene="mnhG"
                     /locus_tag="P5219_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HRX26854.1"
                     /translation="MFLIVGIFIIIGLGFNLLGAFSLYRFPDVYTRLHGATKCTTFGT
                     LFTMFAVVAYSLGRLVSTGEARFAVFIIHGAVAALVLLITNATGAHAIARAAHRSGTL
                     PARAVVDRLEEKEEAKGGTPR"
     gene            complement(582..842)
                     /locus_tag="P5219_08580"
     CDS             complement(582..842)
                     /locus_tag="P5219_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HRX26855.1"
                     /translation="MTIYIFLFAAALVGLLAVAVLGRLVAGPTIADRAVALDTMNTLV
                     VGLMMILAAVYDSVVMVDIAIVYAGLSFVGTMFIARFIEGEV"
     gene            complement(839..1324)
                     /locus_tag="P5219_08585"
     CDS             complement(839..1324)
                     /locus_tag="P5219_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HRX26856.1"
                     /translation="MFIFVVSFLMYLLLVWSGGAIPLIEVLIALILASVVTVAFRSWH
                     RGRKISTRGLDPKRWFGFFHYLFGPFAVGLAKANIDVAKRVITGNIRPGIVKVNPGLT
                     NDVAKTLLADSITLTPGTLTVDVDEEGSFYIHWIYVEDENPTEEQLYGSFGHWARRLA
                     E"
BASE COUNT          421 a          459 c          470 g          361 t
ORIGIN      
        1 atgatgtaaa tcgtggtgct cagtccggct ccgatggcgg cctcggcgat ggccacgtca
       61 ggcgcctgga ggatgtagaa ctccagagcc gtcatgacgc tgaaggcggc cagggagatc
      121 accgaggaaa tgaggtcctt gaaccagagg gcgaagaaag cggacagcac aaggagagcc
      181 agtactacca cgtgcagcat gttgttcatc tgggcgttcc ccctttcgct tcttcttttt
      241 cttcgaggcg gtccaccact gcacgggcag ggagagtgcc gctcctgtgg gcggcccggg
      301 cgatggcatg ggcacccgtg gcgttggtta taagaaggac cagggcagcc acggcaccgt
      361 gaatgataaa gacggcaaaa cgggcttctc ctgttgagac aagcctgccg agagagtacg
      421 caaccactgc gaacatggtg aaaagcgtcc cgaatgtggt gcacttcgtg gcgccgtgaa
      481 gcctcgtgta gacatcggga aacctgtaga gagagaaggc tcccagaagg ttaaagccca
      541 ggccgatgat gatgaagatg ccgacgatga gaaacacgcc gctacacctc cccttcgatg
      601 aaccgggcga tgaacatggt tccgacgaaa gaaagcccgg catagactat ggcgatatcg
      661 accatgacca cggaatcgta gaccgctgca aggatcatca tcaggccgac tacaagggta
      721 ttcattgtgt cgagagctac agctctgtcg gcaatggtgg gcccggcaac aaggcggccc
      781 agcacggcga cggccaacag gccgaccagc gccgcggcga acagaaagat atagattgtc
      841 actccgccaa cctcctcgcc caatggccaa atgatccgta gagctgctct tcggtgggat
      901 tttcatcctc cacgtagatc cagtggatgt agaaactccc ttcttcgtca acgtccacgg
      961 tgagtgttcc cggggtaagg gttatggaat ccgcaagaag cgtctttgcc acatcgttcg
     1021 tcaggccggg gttcaccttg acgatgccgg gccggatatt tcccgttatg acccttttcg
     1081 ccacgtcgat attggctttt gccagaccga cagcgaaggg tccgaacagg tagtggaaaa
     1141 atccgaacca ccttttcggg tcaagtcccc tcgtgctgat tttccttccc ctgtgccagg
     1201 agcgaaaggc cacggtgaca accgacgcca ggatcagtgc gatgagcact tcgatcaagg
     1261 gtatggcccc tcccgaccag acaaggagaa ggtacatcag aaaggagacc acgaaaatga
     1321 acacatactc cacctcccgt cttgattttt tgaatattgc gaatttcgga gacccggaga
     1381 ccaaaaaaac atcggataca ttatactacg gttcgatcca ttgtaaatga aagttaatat
     1441 cctcacaaaa tgtaaacgac gtgcaaaact tccttataaa cagaacggaa aagtggtata
     1501 ctctcccgag ctattttcaa caggagtgaa aaacatggtg ctttctgacg gaatgaagat
     1561 atttctcgcg gatttttctc ttatgcttac cgccctattc tgggggctcg gctttgttgc
     1621 catgaaagat gcccttgaca gtttcccgac cttctggctc ctcgtgctcc gattctcttc
     1681 cggtgctgcg ctcatggcga tagtgttccg g
//
LOCUS       DAOTHD010000507         1711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_83344_length_1711_cov_2.394324, whole genome shotgun sequence.
ACCESSION   DAOTHD010000507 DAOTHD010000000
VERSION     DAOTHD010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83344_length_1711_cov_2.394324"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..321
                     /locus_tag="P5219_08590"
     CDS             100..321
                     /locus_tag="P5219_08590"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRX26857.1"
                     /translation="MLGRNEAIRRTSGRNIPKELSAKGISVMAEKAGTLGEEAPEAYK
                     DVSAVVEVVHGAGLARKVARLRPLAVMKG"
     gene            complement(339..1406)
                     /locus_tag="P5219_08595"
     CDS             complement(339..1406)
                     /locus_tag="P5219_08595"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRX26858.1"
                     /translation="MTITESLFSSNNARDDGGAIDNHFSEISVRRCTFSENTAENGGG
                     IRNYQGKMTVEACTFSGNAAVGDFYGSDIRGGGIYNQGSNTDAVIVNCTFYGNSADFG
                     GGAANWGKMLLINSTLSENSAAHGEELADGKDYLTEVVNCILWHGYPGDEIYTDQSYP
                     VDVRYSIIKGGYPGEGNLDVSPLLGTPSWNGGPTRTCALLPGSPAIDSGTSAGAPEKD
                     QRGIDRPQKSGFDRGAYEVSVRKFLVMRAPFPGVFRFSTATEQFESGEGTAWGFPEGI
                     PVTVTFLPQAGKRLKDVLVDRRSVGAVSSYTFPNLDRDHEIEAVFMETPSSSGGGGCS
                     AGSGFFSFLLLIPLLLIGRRR"
BASE COUNT          358 a          507 c          456 g          390 t
ORIGIN      
        1 tcaacggact tcctgtgctg atccccggaa gcatgggcac ggcaagctac gtcccgaagg
       61 tttccctttc cgcccccggc gtgccacggt gccggaaggg tgctcgggag gaacgaagcc
      121 atccggagaa cctcgggaag gaacatcccg aaggaactct ccgccaaagg catatccgtc
      181 atggccgaaa aggccggaac attgggagaa gaagccccgg aagcctacaa ggacgtttcc
      241 gcggttgtgg aagttgtcca cggtgcgggc ctcgcccgga aagtggcccg tctccggcct
      301 cttgcagtca tgaaagggtg acggacaggt cccccggttc accttctcct gccgatgagc
      361 agcaggggaa tcagcagcag aaaagagaag aatccgctgc cggcggaaca cccgcccccg
      421 ccggaggagg acggggtttc cataaacacg gcttcaatct catggtcccg gtccaggttt
      481 ggaaatgtat aggaagaaac agctccgact gaacgcctgt ccaccagaac atccttcagt
      541 ctttttcccg cttgggggag gaaagttacc gtgacaggaa tcccttccgg gaatccccat
      601 gccgtaccct ctccgctctc gaattgctcc gtggctgtac tgaatctgaa cacaccggga
      661 aagggggctc tcatcacgag aaatttccgg accgacacct cgtaggctcc gcggtcgaac
      721 ccgcttttct gggggcggtc aattcccctc tggtcctttt caggggcgcc ggcagatgtc
      781 cccgagtcta tggcgggact gccgggcaga agtgcgcagg tcctcgtggg gcctccgttc
      841 caggaaggtg ttcccagaag gggggataca tcgaggttgc cttctccggg gtatcctcct
      901 ttaataatgg aatacctcac atcgacggga tatgattgat ccgtataaat ttcatctccc
      961 ggatatccgt gccacaagat gcagttcaca acctccgtga gataatcctt tccgtcggcc
     1021 agttcctctc cgtgagctgc tgagttttcg gaaagcgtgg agtttatcag cagcatcttc
     1081 ccccaattcg ccgcccctcc gccgaaatct gcgctgtttc catagaatgt gcagttaaca
     1141 atcactgcgt ctgtattgga gccctgattg taaattcctc cgccgcgaat gtcgcttccg
     1201 taaaagtcgc ccactgccgc attgcctgag aaagtacagg cttctacagt catttttccc
     1261 tggtaattac gaatgccgcc tccgttttcg gcggtatttt cggaaaaggt acacctcctg
     1321 acggaaattt cactgaaatg gttgtcaatg gcgcctccgt catccctggc attgttggaa
     1381 gagaaaaggc tttctgtgat ggtcatttca ctttcagctg caacaccagt ccccccgacg
     1441 tttgtccagt tatagatgct gccaccgctc ataatggccc tgttcgcgga aaaagtgcat
     1501 ttccggatac gggttacgcc tgacgcgttc cataccgctc ctcccctaaa cccctggttc
     1561 ccggaaaagg tggtttgccg gacttctacc cgtgcgttct ctatgtgaag gcccccggtc
     1621 tggattaccg gatcctctgc ttttatcggg gactctccgt ggagggatgc gtaacatatc
     1681 acctccgaat tcccgccggt gataaccagt c
//
LOCUS       DAOTHD010000508         1710 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_83393_length_1710_cov_3.100906, whole genome shotgun sequence.
ACCESSION   DAOTHD010000508 DAOTHD010000000
VERSION     DAOTHD010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83393_length_1710_cov_3.100906"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1710)
                     /locus_tag="P5219_08600"
     CDS             complement(<1..>1710)
                     /locus_tag="P5219_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26859.1"
                     /translation="YGWPIPDFTVDIDFLQLDFEMSGFITIPGRIETFSGGKGEGGLG
                     FAFGFKLDPFCINTIGGSLELTESHLCILPMTQYNPVAAEWNALGFKISDICDPSKLS
                     IQGNIGFHLVDIAYALDKFAAATGLNLLEGNVAFTLAPAGGVSFSGDVALLRYLPLGD
                     ARISIGWGHPGLSLEGNVYWWGGLFHGVHKGSLSYLNEKIEQTFITEGRIEIPRNIPL
                     IGGYQFANVSAEGLLRGSQSGIEKAWLGARVSFHVVFFDYSVSVGVDFTHPDHPAFFV
                     NGIRVPFSVSAAATEAVARQTVLLDETVPFAYFVVRSEKGLPRANLIFPDGTEHPWET
                     TPTFGPGENPPSAPVCRRENHAIGESYFLVGEPSPGRYTVEIENAQELGTFDIQLILP
                     DNPPEVAFTGLREAITWNGTPLPVEWTVSDDFHPSPKVSLYYGTKEDGSDMRLLSNDP
                     EDRGIYHWDIGAMHIPTGTYRLFAAASDENNPRTIIRSTGTVTVRHPDAPPAPVLAPA
                     DVSPGDGFVEVRWNSVEGGREYIVELHDDRNGVAEIHRVRSVLFSDRDPESGLHSAVI
                     EGLR"
BASE COUNT          343 a          469 c          529 g          369 t
ORIGIN      
        1 cgcagtcctt cgatgacggc tgaatgcagg cccgattccg gatccctgtc cgagaagaga
       61 acgctcctca cacgatggat ttccgcgacc ccgttccggt catcgtggag ttccacgatg
      121 tactcccgcc ctccttccac ggaattccac cgtacttcca cgaatccgtc gccgggagag
      181 acatcggcgg gggcaaggac gggagccggc ggtgcgtccg gatggcggac cgtcacggtg
      241 ccggtgctcc gtatgatggt tcgggggttg ttttcatcgg aggcggcggc aaaaagtctg
      301 tatgttcccg tggggatgtg catcgccccg atatcccaat ggtaaattcc cctgtcttca
      361 ggatcgttcg agagaagccg catgtccgaa ccgtcctctt ttgtgccgta atacagggat
      421 accttcggcg acgggtggaa atcatcgctc acggtccatt ctacggggag gggggttccg
      481 ttccaggtta ttgcctcccg cagtcccgtg aaggcaactt ccggcgggtt gtccgggagg
      541 atgagctgaa tatcgaaggt tccaagttcc tgtgcgtttt ctatctccac cgtgtatctg
      601 ccgggcgaag gctcaccgac gaggaaatag ctttcaccga tggcatgatt ttccctgcgg
      661 catacggggg ctgagggggg attctctccc gggccgaagg tcggcgtcgt ttcccacgga
      721 tgttccgtgc cgtccggaaa aatgaggttg gccctgggta atcctttttc gctccggacg
      781 acgaaatagg cgaaggggac cgtctcatcg agcaggactg tctgcctggc aacggcttca
      841 gtggctgcgg ctgatacgct gaacggaacg cggatgccgt ttacaaagaa cgccgggtgg
      901 tcaggatgtg tgaaatcgac tcccaccgat acgctgtagt cgaaaaagac cacgtgaaag
      961 gagacccggg cccccagcca cgccttttct attccggact gagaaccgcg gagcaggcct
     1021 tccgcgctga cgttggcgaa ctggtatccc ccgatcaggg ggatgtttct ggggatttcg
     1081 atccttccct ccgttatgaa tgtctgctcg attttttcgt tcagatagga gagcgatccc
     1141 ttatggacac cgtgaaaaag gccgccccac cagtacacat tcccctccag ggagagcccg
     1201 gggtgtcccc atcctatgga gatcctcgcg tcgccgagag ggaggtagcg gagcagagca
     1261 acatcgcctg agaatgagac tccgcctgcc ggagcgagcg tgaaggccac gttgccttcg
     1321 agaaggttga gccccgtcgc cgcggcgaat ttgtcgaggg cgtacgcgat gtccacgagg
     1381 tggaatccta tattcccctg gatgctgagc tttgagggat cgcagatgtc gcttatttta
     1441 aacccgagag cgttccattc tgcggcgacg gggttgtact gggtcatagg aaggatgcag
     1501 aggtgtgact ctgtgagttc aagggatccg ccgatggtgt tgatgcagaa ggggtccagc
     1561 ttgaaaccga aggcgaaacc aaggcctccc tcaccttttc ctccggaaaa agtctcgatc
     1621 cggccgggga tggttatgaa gccggacatt tcgaaatcaa gctgaagaaa gtcgatgtcc
     1681 accgtgaagt cgggaatggg ccagccgtat
//
LOCUS       DAOTHD010000509         1710 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_83420_length_1710_cov_2.201208, whole genome shotgun sequence.
ACCESSION   DAOTHD010000509 DAOTHD010000000
VERSION     DAOTHD010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83420_length_1710_cov_2.201208"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1549)
                     /locus_tag="P5219_08605"
     CDS             complement(<1..1549)
                     /locus_tag="P5219_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26860.1"
                     /translation="MRRGLIFLFAAAFFACTFSSPSGAEPKGDIILDADRVTFDQASG
                     MAEAEGTVRLRQDNVRIFSHRMEYDTVNQHAVATSRPGEVVTLLYGGNRFTGKTLEYD
                     MDAGEGVLTDATGDLPAGTRGGTVYIRGKNLEVVPLDSALEKKWIKKRHTRRVKEEDQ
                     QVAKWTDASLTTCPLPKTHYRLMTKRLVVIPGVRVIAKNPRVYIAEKFLFSYPFDYVV
                     PLHGEKDYLLGIFTPSIVYDSDKGVGYALTGPYAWDTGEVTMAFRYWSNIDFEARAGL
                     RQRFGRNLSVFANMDYSWEELEEDEEGRSIGEKKHRPSWGAEYFRQGWSAKILWSQRE
                     NLDLEKRNQVIDGVQQEKNYRNMLHREPEVTVTSPWFSVGGMKDFSWRVTGLWGEYET
                     SRTRPGEARIGTRSVWDVQAQYIVKTGDIRPFWRGQYRKFSYSGFDNPTGRLQDHQET
                     TSMWLGFRTRLGMFDFANAWHIQNVTGRSPLGWDRAGDSEVFYTELGFPVGRDLYLSA
                     MSTYNIKT"
BASE COUNT          311 a          546 c          484 g          369 t
ORIGIN      
        1 gggttttgat gttgtaggtg gacatggcgg aaagatacag gtcgcgcccc acgggaaaac
       61 ccagctccgt gtagaagact tcgctgtcgc cggcccggtc ccaccccagg ggggagcggc
      121 ccgtgacatt ctggatgtgc cacgcgttgg cgaagtcgaa catgccgagc cgcgtccgga
      181 atccgagcca catgctcgtg gtctcctggt gatcctggag cctgccggtg ggattgtcga
      241 agccggaata ggaaaacttc cggtactgcc ccctccagaa aggacggatg tccccggttt
      301 tgacgatata ctgggcctgc acgtcccaga cagacctggt gcctattcgg gcctcacccg
      361 gccgcgtcct ggacgtttcg tactctcccc acagccccgt gaccctccag gagaaatcct
      421 tcattcctcc cactgaaaac cacgggcttg tcacggtgac ttcaggctcc cgatggagca
      481 tgttccggta gtttttctcc tgctggacac cgtcgatcac ctggttccgc ttctcgaggt
      541 ccaggttctc ccgctgggac cagagaatct tggcgctcca tccctgccgg aagtactccg
      601 ctccccagga cggccggtgc ttcttctcgc cgatgctcct tccctcttcg tcctcctcca
      661 gctcctccca tgaatagtcc atgttggcga agacggagag gttccttccg aaacgctgcc
      721 gcaggccggc tcgggcttcg aagtcgatgt tgctccagta gcggaaggcc atggtcacct
      781 cgcccgtatc ccaagcgtag ggaccggtaa gggcataccc gacgcccttg tcggaatcat
      841 agacgatgct cggggtaaag atgccgagca ggtaatcctt ctctccgtgg aggggcacca
      901 catagtcaaa ggggtaggag aagagaaatt tctcggcgat atacacccgg ggatttttcg
      961 ctatgaccct gacgcccggg atcaccacaa ggcgcttcgt catgagacgg taatgggtct
     1021 tcggcagggg gcaggtggtc agggacgcgt cggtccattt cgccacctgc tggtcttcct
     1081 ccttgacccg gcgggtgtgg cgcttcttga tccacttttt ttccagggcc gaatccaggg
     1141 ggacaacttc caggttcttc ccccggatgt agaccgttcc tccccgggtc cccgcaggca
     1201 gatccccggt agcgtcggtt agaacgccct ctccggcatc catgtcgtat tcgagggtct
     1261 ttcccgtgaa gcggttgcct ccgtagagca gcgtcaccac ttctccgggc ctggacgtgg
     1321 ccacggcgtg ctggttcacc gtgtcatact ccatccggtg ggagaaaatc ctgacgttgt
     1381 cctgcctgag acgcaccgtc ccctccgcct cggccattcc gctggcctgg tcgaaggtga
     1441 cccggtcagc atcaaggata atgtcccctt tgggttctgc cccggaaggg gaggaaaagg
     1501 tacaggcgaa gaaagctgcc gcgaacagaa agatcaagcc tcgtctcatg gtattttctc
     1561 cccccggtcg aaccacttca cggtggctcc cctttcagct tccataatcc cgccgggaat
     1621 cagcgtcata tacccggcct cgaatgcacc gagatccccg gaggcctcaa accccttcgg
     1681 gaacacaagg tagtttgttt tctgctccca
//
LOCUS       DAOTHD010000510         1709 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_83489_length_1709_cov_2.671705, whole genome shotgun sequence.
ACCESSION   DAOTHD010000510 DAOTHD010000000
VERSION     DAOTHD010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83489_length_1709_cov_2.671705"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="P5219_08610"
     CDS             complement(<1..723)
                     /locus_tag="P5219_08610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRX26861.1"
                     /translation="MTLHDPMEQVLEIQALSKNFGRVEVLKGISLSLCSGEILALVGD
                     NGAGKSTLLKCVTGTITPDGGVIRLRDKEYLSLSPRRSMAEGVSAVYQDLALVEDLDV
                     ATNLFLGAEPLKAGFLVDRGRMHSEAARRLKELGIDLPSTRARIADLSGGQRQAVAIA
                     RAVMQASGKDRAGIVILDEPTAAMGVRESELVLGIIRGLRERKMAVLFVSHNLPQVFR
                     IADRICVLRSGEMLWCGKTGDVS"
     gene            1035..>1709
                     /locus_tag="P5219_08615"
     CDS             1035..>1709
                     /locus_tag="P5219_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26862.1"
                     /translation="MNRLFRAMKILLLSAVVFPGVPLYSDLLSADEPVVLTRDLKKGL
                     VILVKFPGLDPGIGREEIARRFRRLDRYVKEMSYGKAAVETDLTEWYGMPLPVGKYAI
                     SPSNLKVDKSRVSRLIQDAVDAAGRDHVFSRYTFVVLFLGARFREYGMIGLCGYPGML
                     GWSRSAMLKTSGGQPVPGGVAIFTSQAHLGTLFHDCAHVWGGVRDGKRVLPCLYDHDR
                     QEKYPTR"
BASE COUNT          350 a          506 c          497 g          356 t
ORIGIN      
        1 agaaacatcc ccggttttcc cgcaccagag catctctccg gaacgcagaa cacaaatcct
       61 gtcggcgatc cggaaaactt ggggaaggtt gtgggagacg aagagaacgg ccatttttct
      121 ctccctcaat ccccgaatga tcccgaggac cagttcgctt tcacgaacac ccatggctgc
      181 cgtcggttcg tctaggatga cgatgccggc ccggtccttc cccgatgcct gcatcaccgc
      241 acgggcgatg gcgaccgcct ggcgctgccc tccggaaagg tcggctatcc gcgccctggt
      301 cgaaggaagg tctattccca gttctttgag tctgcgggca gcctccgaat gcatgcggcc
      361 gcggtccaca agaaaccccg ccttcagcgg ttcggcaccg aggaagaggt tcgtcgccac
      421 atcaaggtct tccaccaggg caaggtcctg gtagaccgcc gacactccct ccgccatcga
      481 tctccgggga gagagggaga gatactcttt atcccgcagt cgaataacgc ctccgtccgg
      541 cgtaatcgtt cccgtgacgc atttgagcag cgtcgatttt ccggcgccgt tgtctccgac
      601 aagggcaaga atttctcctg aacaaagaga aagggaaata cctttcagaa cctctaccct
      661 gccaaagttc tttgagagtg cctgtatttc gaggacctgt tccattgggt catgtaaagt
      721 caaagccgac atcaatcctc cgtgccgctc aggaccaaat cccggaacgg cctgctttac
      781 aattgcgaat gaaaattata acaagaaaag atgaaatgga aaaggagacc ggcaacgctc
      841 ctttctcttg cacaaccgcc tttttttctt tttcccgggc gaaatcccag gcctcttctg
      901 tggtatcttt tccctgacgg gccgttccgt tctcaggcga agcctttttc cctcttcgga
      961 aggggagagg atacgctgtt tcgggaagag agggagcggc agggtgattg ggactcaaag
     1021 ggggaggtga cggaatgaac cgtcttttcc gggcaatgaa gattctgctt ctttccgcgg
     1081 tggttttccc gggagtgcct ctgtattccg atctgttgtc cgcagatgaa ccggtggttc
     1141 tcacccggga cctgaagaag ggactcgtga tcctggtgaa gttccccggc ctcgacccgg
     1201 ggatcggcag ggaagagatc gcgaggcggt tccggaggct cgaccgctac gtgaaggaga
     1261 tgtcctacgg aaaagccgct gtggagacgg acctcacgga atggtacggg atgcccctgc
     1321 ccgtggggaa atatgccatt tccccctcga acctgaaggt ggacaagtcg agggtgagca
     1381 ggctcatcca ggatgccgtg gacgccgccg gcagggatca tgttttttcc cgctacactt
     1441 tcgtcgtcct gttcctgggg gccagattcc gggagtacgg catgatcggc ctctgcggat
     1501 atccgggaat gctcgggtgg agccggtccg ccatgctgaa gacgagcggc ggccagcccg
     1561 tccccggcgg agtggccatc ttcacctccc aggcccacct ggggaccctc ttccacgact
     1621 gcgcccacgt ctggggcgga gtgcgggacg gaaaacgggt tcttccctgc ctgtacgacc
     1681 acgaccgcca ggagaaatat cccacccgg
//
LOCUS       DAOTHD010000511         1709 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_83490_length_1709_cov_2.633615, whole genome shotgun sequence.
ACCESSION   DAOTHD010000511 DAOTHD010000000
VERSION     DAOTHD010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83490_length_1709_cov_2.633615"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="P5219_08620"
     CDS             <1..743
                     /locus_tag="P5219_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX26863.1"
                     /translation="RAVDGFSFGVTEGSITGIIGPNGAGKTTVFNLVTGIYRPTEGTI
                     LFEEEEMGGLRPDAIVRKGIARTFQNIRLFNRRTCLENVLTPLLQREPCSFLGALLGT
                     PGTRRMERELHDRAMDLLASLGLERYADWQANTLPYGLQRKLEIARALATEPKLLLLD
                     EPAAGMNPEETRSLAALVSEVREKFSVTILLIEHHMDLVMNICSPIVVMNFGALLAAG
                     TPDEVRANPGVVAAYLGKGKKSHDRTAG"
     gene            724..1431
                     /locus_tag="P5219_08625"
     CDS             724..1431
                     /locus_tag="P5219_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX26864.1"
                     /translation="MTVLRVDSLHVHYGTIHAVQGISFSLEEREIVSIVGSNGAGKST
                     VMWTLAGVAERSGGTVELFGRPLPVKPHEVVARGLALVPERRRLFSALTVEENLVMGA
                     YRRKKDERVAEDMERCFRLFPILKQRLKQRSGTLSGGEQQMLAISRALMGSPRILLLD
                     EPSLGLAPIVVDTLMETILQIRDGGMTVLLVEQNATQALEISDRGYILEAGRFIKSGS
                     GPELAGDPEIIRAYLGG"
     gene            complement(1409..>1709)
                     /locus_tag="P5219_08630"
     CDS             complement(1409..>1709)
                     /locus_tag="P5219_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26865.1"
                     /translation="YGTIAALRKQSPDFISVTYGAWGSSRERTGEIASATRGYPEGDV
                     RRFIELLKKFRTGKEGDGEGFSALFGKGPGISGKDCAAGSDLRRRFFSFSPRGRP"
BASE COUNT          316 a          549 c          538 g          306 t
ORIGIN      
        1 tccgggcggt ggacggattt tccttcgggg tcactgaagg ctccatcacg ggaatcatcg
       61 gccccaacgg cgcggggaag accacggtgt tcaatcttgt cacgggcatc taccgcccca
      121 ctgaggggac catcctcttc gaagaggagg agatgggagg gctccgtccc gatgccatcg
      181 tcagaaaggg catcgccagg acgttccaga atatccgcct cttcaacagg cgaacctgcc
      241 ttgaaaatgt cctcaccccc ctcctccaga gggagccgtg cagtttcctc ggcgccctcc
      301 tggggactcc gggaacccgg cgcatggaaa gggagctcca cgacagggcc atggatctcc
      361 tggcgtccct gggcctcgaa cggtacgcgg actggcaggc gaacaccctg ccttacggcc
      421 tccagcgcaa gctggagatc gcccgggccc tcgcaacgga gccgaagctt ctgcttctgg
      481 acgagcctgc ggccggcatg aaccccgagg agacccggag tctcgccgcc ctcgtctccg
      541 aggtccggga gaaattcagc gtgaccatcc tgcttatcga gcaccatatg gacctggtga
      601 tgaacatctg ctcacccatc gtggtgatga acttcggtgc cctcctcgcc gcgggaacgc
      661 cggacgaggt ccgggcgaac cccggtgtgg tggcggcgta cctcgggaaa ggaaagaaga
      721 gccatgaccg tactgcgggt tgacagcctc cacgtccatt acggcaccat ccatgccgtc
      781 caggggatct ccttctccct cgaggagcgg gagatcgtgt ccatcgtcgg ctccaacggg
      841 gccggaaaat ccaccgtcat gtggaccctg gcgggggtgg cggaacggtc cgggggtacc
      901 gtggagcttt tcggcaggcc ccttccggtg aagccccatg aagtggttgc ccgggggctg
      961 gccctcgtgc ctgaaaggcg ccgtctcttc tcggccctca cggtggagga aaacctggtc
     1021 atgggggcgt accggagaaa gaaggacgaa cgggtcgcgg aggacatgga acgttgtttc
     1081 cgcctctttc ccatcctgaa gcagcgtctg aagcagcgtt ccggcaccct ttccgggggg
     1141 gagcagcaga tgctggccat ctcgagggcc cttatgggaa gtcccaggat cctccttctg
     1201 gacgaaccct ccctcggcct ggcgcccatc gtggtggaca ccctcatgga gaccattctg
     1261 cagatccggg acggggggat gacggtgctg ctcgtggagc agaacgccac ccaggcgctg
     1321 gaaatcagcg acaggggcta catcctcgaa gcggggcggt tcatcaagag cggcagcgga
     1381 ccggaactcg ccggggatcc cgagatcatc agggcctacc tcgggggctg aacgaaaaaa
     1441 agcgccgccg gagatcactc ccggcggcgc agtctttccc tgaaatcccc ggccccttcc
     1501 cgaaaagagc cgaaaatcct tctccatccc cttctttccc tgtccggaat tttttcagca
     1561 gttctatgaa gcggcggacg tctccttccg gatagcccct cgtggctgag gcgatctccc
     1621 cggtcctctc cctgctgctt ccccaggcac cgtaggtgac gctgatgaaa tcgggggact
     1681 gcttcctgag ggcagcgatg gtgccgtag
//
LOCUS       DAOTHD010000512         1709 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_83491_length_1709_cov_2.616082, whole genome shotgun sequence.
ACCESSION   DAOTHD010000512 DAOTHD010000000
VERSION     DAOTHD010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83491_length_1709_cov_2.616082"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="P5219_08635"
     CDS             complement(<1..186)
                     /locus_tag="P5219_08635"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRX26866.1"
                     /translation="MSLINVSNLTFGYEGGLEKIFENVSFRVDTDWKLGFTGRNGRGK
                     TTFLNLLLGRYDYQGSIT"
     gene            complement(259..1128)
                     /locus_tag="P5219_08640"
     CDS             complement(259..1128)
                     /locus_tag="P5219_08640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRX26867.1"
                     /translation="MDSDFSLLIGYQDFEPGRRSFNALARKTFGLDFEPWYRSGYWTK
                     QYIPYSLSRDGEVVSSVSVNFMPMTLEGTGLTMVQIGTVMTDPAFRGQGLSRFLMETV
                     LDEWSSKVDEVYLFANDEVLDFYPRFGFRKSGEVQCTASVSTPFPARAEKVDMEQPKH
                     REKVERAIRATRGVSRFSMNNAGLAMFYLTGPMQDRVRYDPETGAYLVAAVESDLLRL
                     DDVFCEEAVDLDCAVAAFGPEIRRVVFGFSPCMDKGLDRTACEEEDTTLFVLKNAVDF
                     RAKGLRFPFLSRA"
     gene            1269..>1709
                     /locus_tag="P5219_08645"
     CDS             1269..>1709
                     /locus_tag="P5219_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26868.1"
                     /translation="MITASREWRTSFPGACFGVLVLEGVDNSRECPALEERKSAFETA
                     LRSRHEGKSRKEIGLEEPFGSYGRYYKRFGQGYPVLHQVETVALKGKPLFSPSPLVAA
                     MFTAELKNGFLTAGHDLDTVSLPLFLDVSKGEEGYTAMGGRERIL"
BASE COUNT          362 a          524 c          491 g          332 t
ORIGIN      
        1 cgtgatggaa ccttggtagt cgtaccggcc gagcagcagg ttcaggaacg tggtctttcc
       61 ccggccgttg cggccagtga agcccagctt ccagtccgtg tcgacccgga agctcacgtt
      121 ctcgaaaatc ttctccagtc cgccttcgta gccgaaggtg aggtttgaaa cattgatcag
      181 agacatgcat atcctccgaa aaacaggaaa aaagcactcc ggcggtccgg agtttgcatt
      241 cccttccgcc gggctcgttc aggcccggga caggaagggg aatctcagcc cttttgcccg
      301 gaagtcgacg gcgttcttca ggacgaacag ggtagtgtcc tcctcttcgc aggccgtccg
      361 gtcgagcccc ttgtccatgc agggcgaaaa tccgaagacc acccttcgga tctccggacc
      421 gaaggcggcg acggcacaat ccaggtcaac cgcttcttcg caaaaaacgt cgtccagcct
      481 gagaagatcg ctttccacgg ccgcgacgag ataggctccc gtttccgggt cgtaccggac
      541 ccggtcctgc atcggccctg tgaggtaaaa catggcgagt cccgcattgt tcatgctgaa
      601 cctgctcact ccacgggtcg cccgtatggc ccgctccacc ttttcccggt gtttgggctg
      661 ttccatgtcc accttttcag cccgggcggg aaaaggtgtc gagacggagg cggtacactg
      721 cacttccccg ctcttccgga agccgaaccg gggatagaaa tccagcactt cgtcgttggc
      781 gaagagatag acttcatcca ccttcgacga ccattcgtcc aggacggtct ccatgaggaa
      841 ccggctgagc ccctgccccc ggaaggcggg atcggtcatc accgtgccga tctgcaccat
      901 ggtgaggcct gtcccttcca gcgtcatggg catgaagttc accgacacgc tggagaccac
      961 ctccccgtcc cgggacaggg aataggggat gtactgcttc gtccagtacc ccgacctgta
     1021 ccatggctcg aagtcgaggc cgaaggtctt ccgggccagc gcattgaagg agcgccgtcc
     1081 cggctcaaaa tcctggtagc ctatcaggag agaaaaatca ctgtccatag ccttattctc
     1141 cttgatgtcc cgactcgacc ggtccccggc ctgtcatcct gtccggggca aaaacatgct
     1201 gtccggaaaa aatcctcatt ctcaggtatc ataaacgaaa acgcacagga aaaggagttg
     1261 atcacatcat gatcaccgca tcaagagagt ggaggacgag ctttcccggc gcctgtttcg
     1321 gtgtgctcgt gctggaggga gtggacaatt cgcgggaatg ccccgccctc gaggagagga
     1381 aaagcgcctt tgaaaccgcc ctcaggagcc gccacgaagg aaagagcaga aaagagatcg
     1441 gccttgaaga gcccttcggg tcctacgggc ggtactacaa aaggttcggc cagggatatc
     1501 ccgtgctgca ccaggtggaa accgtggcgc tgaagggaaa gcccctcttt tcgccgtccc
     1561 ccctcgtggc ggccatgttc acggcggaac tgaagaacgg ctttctgacc gccggccacg
     1621 acctggacac tgtttccctg ccccttttcc tggatgtctc gaagggggag gaaggctata
     1681 cggccatggg aggcagggaa cggatcctc
//
LOCUS       DAOTHD010000513         1706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_83706_length_1706_cov_2.340400, whole genome shotgun sequence.
ACCESSION   DAOTHD010000513 DAOTHD010000000
VERSION     DAOTHD010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83706_length_1706_cov_2.340400"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="P5219_08650"
     CDS             complement(<1..264)
                     /locus_tag="P5219_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRX26869.1"
                     /translation="MDIRLAGVTKVFEPDIVALEDIYLSIDKGEFVYLVGTTGSGKTT
                     LLRLISREYLPTRGQITVGDVNLRKIGRGRLPFYRRDVGVVFQD"
     gene            complement(268..1218)
                     /locus_tag="P5219_08655"
     CDS             complement(268..1218)
                     /locus_tag="P5219_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRX26870.1"
                     /translation="MPAPERSTRDAYGQALIDLAAADPSVVAVDGDVGSATRAARFAA
                     LHPDRVFNVGMAEQDMILTAAGLALGGKNVYVSSYASFLVGRGYEQIRSAVAIPGLPV
                     KIVGSHCGVTVGEDGASHQMLEDLALMRVFPDMTVLVPADYTSALALLKNAVSSRGPV
                     YVRLGRAPVPDVYADGDEGFLPGGGRVLREGTEVTVCCCGIMVSEALRAAEVLARQNI
                     SAEVIDCYSVSPLPAQSILDSVHRTGCCVTAEEHFARGGLGEGVAALVSGAYPVPVKQ
                     VAVFDKFGQSGSPAELQEYYGLTASQIVSAAVQAWTMRRR"
     gene            complement(1222..>1706)
                     /locus_tag="P5219_08660"
     CDS             complement(1222..>1706)
                     /locus_tag="P5219_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRX26871.1"
                     /translation="GGSLGQGLGLANGLALALRDRASVPSVFCLVGDGELQEGAFWES
                     AMTSAHFSLDNLLLLVDRDGRQMEGATEDIMSLEPVRDKFLAFGWAVEECDGHDFASM
                     EEARARLGSRRGKPGCIVANTVLGKGVSFLENDPSGGRMVLGRDSMDKALRELEKGGD
                     "
BASE COUNT          315 a          579 c          520 g          292 t
ORIGIN      
        1 atcctggaag acgaccccca cgtccctcct gtagaaggga agcctgcccc gtccgatttt
       61 tcgcagattc acgtcaccca cggtgatctg gcccctggtg ggaaggtatt cccgggagat
      121 gagcctgagg agggtggtct ttccagagcc tgtggtcccc acaaggtaga cgaactcccc
      181 cttgtcgatg gagagataaa tatcctcgag ggcgacgatg tccggttcga ataccttggt
      241 gactcctgcc aggcgaatat ccatttctta cctcctgcgc atggtccagg cctgcactgc
      301 ggcactcacg atctgggatg ccgtcaggcc gtaatactcc tgcagctccg ccggagaacc
      361 gctctggccg aacttgtcga atacagcgac ctgcttcacc gggacggggt aggcgccgct
      421 cacgagggcg gcgacacctt ctcccaggcc tccccgggca aaatgctcct cggcggtgac
      481 gcagcacccc gtcctgtgga cggagtcgag aatgctctgg gccggaagag gggatacgct
      541 gtagcagtct atcacctcgg cgctgatgtt ctgccgcgcc agcacttccg cagcccggag
      601 cgcctcggag accatgatac cacaacagca gaccgtcact tccgtcccct cccggagcac
      661 ccttcctcct ccgggaagga agccttcgtc accgtcagcg taaacgtccg gaacgggggc
      721 cctgccgagc ctgacgtaca cgggccccct ggaggagaca gcgtttttca gcagcgcaag
      781 ggcagatgtg taatcagccg ggacgaggac cgtcatgtcc gggaagactc tcatgagagc
      841 caggtcctcg agcatctggt gggacgcccc gtcctccccc acggtaaccc cgcagtggct
      901 ccccacaatc ttcacaggga ggccggggat ggccacggcg gaccggatct gctcgtaccc
      961 ccgccctacg aggaaggaag cgtaggagga cacgtagacg ttctttcccc cgagggccag
     1021 tccggcagcg gtgaggatca tgtcctgctc cgccatgccc acgttgaata ccctgtcggg
     1081 atggagggcg gcaaaccgcg ccgcacgggt ggcggatccc acgtcgccgt ccacggccac
     1141 caccgaaggg tctgccgcgg cgaggtcaat aagggcctgc ccgtaggcgt ccctggtgct
     1201 tctttcagga gccggcatcg ttcagtcccc tcccttttcg agttcccgga gggccttgtc
     1261 catggaatcc cgccccagga ccatccgccc tccggaggga tcgttttcga ggaaggaaac
     1321 accctttccg agcacggtgt tcgccacgat gcaccccggt tttcccctgc gggacccgag
     1381 gcgggcacga gcctcctcca tggaggcgaa gtcatggccg tcacactcct ccacggccca
     1441 gccgaaggcg agaaacttgt cccggaccgg ctcgagggac atgatgtcct ccgtggcccc
     1501 ctccatctgc cgtccgtccc tgtccaccag gagcagcagg ttgtccaggg agaagtgggc
     1561 ggaggtcatg gcagactccc agaaggctcc ctcctggagc tcaccgtcgc ccacaagaca
     1621 gaagaccgat ggaacggatg cccggtccct cagggcaaga gcgaggccgt tggcgaggcc
     1681 gagcccctgg ccgagggatc ctccgg
//
LOCUS       DAOTHD010000514         1704 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_83817_length_1704_cov_2.685870, whole genome shotgun sequence.
ACCESSION   DAOTHD010000514 DAOTHD010000000
VERSION     DAOTHD010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83817_length_1704_cov_2.685870"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="P5219_08665"
     CDS             complement(<1..427)
                     /locus_tag="P5219_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRX26872.1"
                     /translation="MSKAIAARSLCKSYRGRTVVSEVDLDVHMGEIVGLLGPNGAGKT
                     TTFYMIVGLVKPDSGAVSINSRELTALPVYRRARLGVGYLPQEASIFRNLTVRENLEL
                     VLQENPPPEEPAKNVVDRLIEDFGLQKITNIPGYSLSGGE"
     gene            complement(424..1161)
                     /locus_tag="P5219_08670"
     CDS             complement(424..1161)
                     /locus_tag="P5219_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26873.1"
                     /translation="MKYVRFFVFFVLLALLAVSPLFAEDLELTSDKMRYESETGDFWA
                     DGNVKVTRGTIMATSKTADGNMNRQIFTMKGGVHVFGSWQKEKVDMKGQTLSGVFTEP
                     QEYIFDGGVKGFWGSRQVDADKLRMKGDYFWGTKLRRYADTKEGYVLVCDALEGKLEN
                     GEISEFTAAGNVYFLSSPKKGDPTEIRGSKAVYSKARGTLVVSGGVTALQAARSLKSE
                     TLIFFPDRNRVEASGKPRLIFKTGGSK"
     gene            complement(1225..>1704)
                     /locus_tag="P5219_08675"
     CDS             complement(1225..>1704)
                     /locus_tag="P5219_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRX26874.1"
                     /translation="DGSGRCVVEEVISDSDVAKIAVLGVPDVPGVAARLFSDLAAAGV
                     GAEMIVQSVMRGQMNDIAFIVKQSLLGDAIAICRSFASGVGAQGVTFDAEVAKVALKG
                     DLLSGCPEIPSQMFSVLAGCRINIDMIAAASTIIACIVASPDMEGAASALSQSFLRS"
BASE COUNT          331 a          532 c          498 g          343 t
ORIGIN      
        1 gctctcctcc gctgagggaa tatccgggga tgttggtgat cttctggagg ccgaaatcct
       61 ctatcagcct gtccacaacg ttcttcgccg gttcttcggg gggagggttc tcctggagca
      121 ccagttcaag gttttccctc acagtgaggt tccggaagat ggacgcctcc tgggggagat
      181 agcccacgcc gagacgggcc ctgcggtaga ccggcagggc cgtcagttcc cgggaattga
      241 tcgagaccgc tcccgaatcg ggcttgacga gcccgacgat catgtagaat gtggtggttt
      301 ttcccgcacc gttcgggccg agcaggccca cgatctcgcc catgtggacg tcgaggtcaa
      361 cttcagaaac caccgtgcgg cccctgtagg acttgcagag ggaccgggcg gctatcgcct
      421 tgctcatttt gatccgcccg tcttgaaaat aagccggggt ttgcccgaag cctccacccg
      481 gttccggtcg gggaagaata tcagggtctc cgatttcagg gacctggcag cctggagggc
      541 ggtcacgccg ccggacacca ccagggttcc cctcgccttt gaatagacgg ccttgctccc
      601 ccggatctcc gtggggtcac cctttttcgg cgaggaaaga aaatagacgt tcccggcagc
      661 ggtgaattcg gatatttcgc cgttctcaag cttcccctcc agggcgtcgc agacgagaac
      721 gtatccttcc ttcgtgtcgg cgtaccggcg gagtttcgtt ccccagaagt agtcgccctt
      781 catcctgagc ttgtccgcgt caacctgacg ggacccccag aagcccttca cgccgccgtc
      841 gaaaatatac tcctggggct cggtgaacac gcccgagagg gtctggccct tcatgtccac
      901 cttctctttc tgccaggagc cgaagacgtg gacaccgccc ttcatggtga agatttgccg
      961 gttcatgttc ccgtccgccg tcttggaggt ggccatgatg gtaccccggg tgaccttcac
     1021 gttcccgtcg gcccagaaat cccccgtttc cgactcgtac cgcatcttgt cggaggtgag
     1081 ctccaagtcc tcggcaaaca ggggggaaac ggcgaggaga gccagcagaa caaagaaaac
     1141 gaaaaagcga acatatttca tgggacagcc tcccatacgg tatttttccc tattgtacag
     1201 gaattttact ctccgccata ggggtcagga gcggagaaac gactgtgaaa gggcggaagc
     1261 ggctccctcc atgtcggggg aagccacgat acaggcgatg atcgtggagg ccgccgctat
     1321 catgtcgatg ttgatcctgc atcctgccag gacggagaac atctgggacg gaatctccgg
     1381 gcagccggag aggagatccc ccttcagagc cacttttgcc acctccgcgt cgaaggtaac
     1441 cccctgggcg ccaacgccgg aagcgaagga cctgcagatg gcgatggcat ccccgaggag
     1501 gctctgcttc acgatgaagg caatgtcgtt catctgcccc ctcatgacgc tctggacgat
     1561 catttccgct cccacccctg cagcggcaag gtcggagaag agccgggccg ctactcccgg
     1621 aacgtcgggc acgccgagaa ctgcgatctt cgccacgtcg ctgtcgctga tcacttcctc
     1681 cacgacgcac ctgccggaac cgtc
//
LOCUS       DAOTHD010000515         1697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_84295_length_1697_cov_3.165043, whole genome shotgun sequence.
ACCESSION   DAOTHD010000515 DAOTHD010000000
VERSION     DAOTHD010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84295_length_1697_cov_3.165043"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="P5219_08680"
     CDS             complement(<1..216)
                     /locus_tag="P5219_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HRX26875.1"
                     /translation="MRVLVALGGNAILQRGQKGNAAEQRDNVQKTVDQIVRMIRAGHE
                     VVVTHGNGPQVGAILIQNELGSASVPAM"
     gene            complement(276..1121)
                     /gene="speE"
                     /locus_tag="P5219_08685"
     CDS             complement(276..1121)
                     /gene="speE"
                     /locus_tag="P5219_08685"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HRX26876.1"
                     /translation="MDTRPKRFNELWITEEQSPDMKLSLRVSDVLLNVKSPYQDILLV
                     DTGEYGRMMILDGAIQITERDEFCYSEMMAHVALSSHPDPKRVLIVGGGDGGVLREVL
                     RHKNVEKATLIDIDEDVINASKRFLPTISAALEDPRADVKPMDAMVYIKAAKEEFDIA
                     IVDSTDPVEFAAGLFESPFYRDIHTALKKDGMVVAQTESPFTDRNVVRDAFREMSSVF
                     PVVRMYWGAMPTYPSGMWTYTVGSKTADPSVPLRPAPEGTKYYTSEIHRASFVLPPFV
                     IDLLK"
     gene            complement(1218..>1697)
                     /locus_tag="P5219_08690"
     CDS             complement(1218..>1697)
                     /locus_tag="P5219_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HRX26877.1"
                     /translation="NYISPTKETSVYGTEIIRFLKEDFGENLPEILLEPGRFMAGDAG
                     ILITEVVLISRKSEANQYSWVYLDAGKFGGLIETLDESIKYPIYSEKTGPIQETILAG
                     PTCDSMDILYENEKYSLPASLSEGDRLYFFTTGAYTQSYSSVCFNGFPPLRAYVLED"
BASE COUNT          336 a          444 c          483 g          434 t
ORIGIN      
        1 catggcggga accgaagcac tgcccagttc gttctggatg aggatagctc cgacctgggg
       61 gccgttgccg tgggtgacca caacctcatg accggcccgt atcatcctga cgatctggtc
      121 cactgttttc tgtacattgt cccgctgttc cgctgcattg cctttctggc ctctctgcag
      181 aatggcgttg cctccaaggg cgaccaaaac tctcatctct ttcaattccc ctcctgaatg
      241 ttaatgataa accaatatat tctaaactaa gcagcttatt tcagcaagtc aatgacaaag
      301 ggaggaagaa cgaaactagc ccggtggatt tcggacgtgt agtatttcgt tccctcaggg
      361 gccgggcgaa ggggtaccga cgggtctgcg gtttttgacc ccacggtgta ggtccacatt
      421 ccgctgggat aggtgggcat ggccccccag tacatcctga cgacggggaa gacggaactc
      481 atttcccgga aggcgtcccg gacgacgttc cggtccgtga agggcgattc tgtctgggca
      541 acgaccattc cgtccttttt cagggcggtg tggatatcgc ggtagaaggg tgattcgaag
      601 agcccggcgg caaattccac gggatcagtg ctgtccacga tggcgatgtc gaattcttcc
      661 tttgcggctt tgatgtacac catggcgtcc attggtttta catcggccct ggggtcctca
      721 agggcagcgc tgatagtcgg gaggaaccgt tttgaggcgt tgatcacatc ttcatcgata
      781 tcgatcaggg tcgccttttc cacgtttttg tggcggagga cttcccgcag gacgcccccg
      841 tcaccgccgc cgacgatgag cactcttttc ggatcagggt gcgatgagag cgccacgtgg
      901 gccatcattt cggaatagca gaattcatcc cgttccgtta tctggatggc tccgtcaaga
      961 atcatcattc tgccgtattc tcctgtgtcc acgagcagga tgtcctggta ggggcttttc
     1021 acgttcagaa gcacatctga tacgcggagg ctcagcttca tgtccgggct ctgttcttcc
     1081 gtgatccaga gttcgttgaa tcgtttcggg cgggtatcca ttgggcattc tcctttccct
     1141 ggaaagagcg gagggaacaa gtcgttcaac ctgttccctc cgctgtctga ctttcattca
     1201 gtatagaatg tcctgcttta atcttcaagc acgtaggcgc ggagcggcgg gaagccgttg
     1261 aagcagaccg aggaatagct ctgcgtgtag gctccggtgg tgaagaaata gaggcggtct
     1321 ccttccgaaa gtgatgcggg gagggagtac ttttcgtttt cgtacagaat gtccatgctg
     1381 tcgcaggtgg gaccggccag gatcgtttcc tggatggggc ctgttttttc cgagtagatc
     1441 gggtatttta tggactcgtc gagcgtttcg ataagcccac cgaatttacc tgcgtcaagg
     1501 tagacccagc tgtactggtt ggcttccgac ttccgggaaa tgagcaccac ttccgtaatg
     1561 agaattccgg cgtccccggc catgaacctg ccgggttcaa gcagaatttc cggaagattt
     1621 tctccgaaat cctccttgag gaaccggatg atctccgttc cgtagactga ggtctccttc
     1681 gtgggagaga tgtagtt
//
LOCUS       DAOTHD010000516         1697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_84313_length_1697_cov_2.621803, whole genome shotgun sequence.
ACCESSION   DAOTHD010000516 DAOTHD010000000
VERSION     DAOTHD010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84313_length_1697_cov_2.621803"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="P5219_08695"
     CDS             complement(<1..294)
                     /locus_tag="P5219_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRX26878.1"
                     /translation="MDEQQHETAVTGFLEKLKNRGLRLTAQREVLFRVIAENIGIPST
                     VQDIWGKTRDLDPSIGIATVYRTINLLAEMGMVNVIYLNEGQFRLEMPEQKLHV"
     gene            397..657
                     /locus_tag="P5219_08700"
     CDS             397..657
                     /locus_tag="P5219_08700"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HRX26879.1"
                     /translation="MPARNGTGPLGMGPMTGWGRGWCAGGRGMGRGFAGYGRFGMGRG
                     FGGVWGSPVTPEMEEQVLRQEMEMLKARMDELQARLGKDSKE"
     gene            654..1016
                     /locus_tag="P5219_08705"
     CDS             654..1016
                     /locus_tag="P5219_08705"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HRX26880.1"
                     /translation="MTKAAIALDGSVVSEHFGKVREFLFLTFENGREISREVLPAPTA
                     DHAPGVFPNWVKSMGADVVMAGGMGVKAKQFFETLGVQVLTVPPMDVEEGVKALLLGT
                     VRTVETDCGHGSDHDCGK"
     gene            1013..>1697
                     /locus_tag="P5219_08710"
     CDS             1013..>1697
                     /locus_tag="P5219_08710"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRX26881.1"
                     /translation="MKIAVASGKGGTGKTSISASLVLARPENLALDLDVEEPNLSILL
                     GAFEGGVLPVSMPIARIEGSKCVECGKCARSCVFGAIAWMGAGFPVINDSLCRGCGLC
                     PRVCPTEAIYEEDQTVGEVRSGTCGGIPFLEGRLRVGSVNTVRVIEETVKKAKAFNRP
                     DWLMDCPPGTACPVAASLRHADMALLVTEPTPFGRSDLEGMLELTADMNIPTALVINK
                     TGIGDLSLDD"
BASE COUNT          342 a          440 c          554 g          361 t
ORIGIN      
        1 cacgtggagc ttctgctcag gcatctcaag ccggaactgt ccctcgttca ggtagatgac
       61 attcaccatg cccatctcgg cgagaaggtt gatggtccgg tagacggtgg cgatgccgat
      121 ggacgggtcg agatccctcg tctttcccca gatgtcctgt accgtggaag gaatcccgat
      181 attttcggcg atcacccgga aaaggacctc ccgttgggcc gtgaggcgga gtcccctgtt
      241 cttcagtttt tcaagaaagc cggtcacagc ggtttcgtgc tgttgttcgt ccatttgttt
      301 cctcctgggg cagatcgtgt ttttcctctt gacataatgg cacaaagggg tctattatgc
      361 aactgataat cagtatcatt aaaggaggaa tgaataatgc ctgcacggaa cggaacggga
      421 cccctcggaa tggggcccat gacaggttgg ggacgaggat ggtgtgccgg aggcagaggt
      481 atgggccgcg gtttcgccgg atacggcagg ttcggcatgg ggcgtggttt cggcggcgta
      541 tggggatctc ccgtcactcc tgaaatggaa gagcaggttc ttcggcagga gatggagatg
      601 ctgaaagcca ggatggatga gcttcaggct cggctcggaa aggacagcaa ggaatgacga
      661 aggcggccat cgctcttgac ggcagcgttg tttccgagca tttcggaaag gtccgggagt
      721 ttcttttcct tacctttgaa aacggaaggg agatttccag ggaggttctt cctgccccca
      781 cggctgatca tgcgccgggc gtttttccca actgggtgaa atccatgggg gcggacgtgg
      841 ttatggcggg gggcatgggc gtcaaggcca agcagttttt cgagaccctc ggagtccagg
      901 tcctcacggt gcctcccatg gatgttgaag agggtgtgaa agcccttctt ctgggaacag
      961 tccggacagt ggaaaccgac tgcggtcacg gttcggatca cgactgcggc aaatgaagat
     1021 agcggtggcc agcggcaagg gaggcacggg aaagacaagc atctcggcct cccttgtgct
     1081 tgcacgcccg gaaaacctcg cgcttgatct cgatgtggag gagccgaacc tgagtattct
     1141 cctcggtgcc ttcgaggggg gcgtgttgcc cgtctcgatg cccatcgcca ggattgaagg
     1201 aagcaagtgc gttgaatgcg ggaaatgcgc ccggagctgt gtcttcggag ccatagcgtg
     1261 gatgggtgcg ggattccccg tcatcaacga ttcgctgtgc cggggatgcg gcctttgtcc
     1321 cagggtttgc cccacggaag ccatatatga ggaggatcag accgtcgggg aagtccggtc
     1381 cggaacctgc ggcgggatcc cttttctcga agggcggctc cgggtcggga gcgtcaacac
     1441 agtccgcgtc atcgaggaaa cggtaaaaaa ggcgaaggcg ttcaaccggc ccgactggct
     1501 gatggactgc cctccgggaa cggcctgtcc tgtcgcggca tcactccgcc acgcggacat
     1561 ggcccttctg gtgacggagc ctacaccgtt cggacggtcc gatctggagg gaatgcttga
     1621 attgacggcg gacatgaaca ttccgacggc tctcgtgatc aacaaaacgg gaatcggcga
     1681 tctctctctc gacgacc
//
LOCUS       DAOTHD010000517         1695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_84460_length_1695_cov_2.525610, whole genome shotgun sequence.
ACCESSION   DAOTHD010000517 DAOTHD010000000
VERSION     DAOTHD010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84460_length_1695_cov_2.525610"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..1658)
                     /locus_tag="P5219_08715"
     CDS             complement(138..1658)
                     /locus_tag="P5219_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HRX26882.1"
                     /translation="MSERHAEPGAVNRKIVANCIDPEETRVAIIEDGRLADLFVERMW
                     ERQKAGEIYKARVESVLPGIHASFVNLGDGRNAFLYLNDARGLNLQPNQELLVQVTKT
                     ARKNKGARVTSRISLPGRYVVLVPHGQESGVSKRITDENERRRLKTVARELRGEDYGI
                     IVRTAAEGVDEESLAHDVEVLLALWREIEHNGRIQSAPCLLYRDLGLLGRVLRDELTD
                     AVSEIVVDGREEYENVNNSLSLFSGTDHPEVSLYGGTTPLFEFYGIEKEIEAALERKV
                     WLQSGAYLIIDHTEALTVIDVNTGKFIGKTDLRHTVLETNLEAASEIARQLRLRAIGG
                     IVVIDFIDMDYEEDRTLLLKRLEEVFLPDRYRARIFGVSQLGLVEITRKRARPDIRST
                     LTRSCPFCSGNGWVHREDTISMTIKRFLRKVSASNRSEALLLEANPAVAHYIYETYLS
                     LWEEELGRRIFLAAVPEFAWSKFRLDAQGGLEQVERKMEQMEKWEARLVVYRTSSS"
BASE COUNT          288 a          548 c          507 g          352 t
ORIGIN      
        1 tctggaatcc cccgtcgcca agcacccacc ggagggcgtc gagaaggttg ctctttccgc
       61 tgccgttggg ccccacgatg gcggtgagcc ccggcgagaa caccaggtcc tgggaagcac
      121 cgaaactttt gaacccttta agatgacgat gttcgatata caacgagacg ggcctcccat
      181 ttttccatct gttccatttt ccgctccacc tgctcgaggc ctccctgggc gtccagccgg
      241 aacttgctcc aggcgaactc cggaaccgcc gcgaggaaga tcctgcggcc gagttcctcc
      301 tcccagaggg agagataggt ttcgtaaatg tagtgggcca ccgcggggtt cgcctccagc
      361 agaagggcct ccgagcggtt cgacgccgac actttcctga ggaaacgctt gatggtcatg
      421 gagatggtgt cctcacggtg aacccacccg ttgccgctgc agaagggaca gctcctggtg
      481 agggtggacc tgatgtcggg ccgggcccgc ttcctggtga tctccacgag cccgagctgg
      541 gatactccga aaatgcgcgc ccggtaccgg tcgggcagaa agacctcttc caggcgcttc
      601 aggaggaggg tccggtcctc ctcgtaatcc atgtcgatga agtcgatgac cacgatgccg
      661 ccgatggccc gcagccggag ctgacgggcg atctcggacg cggcctcgag attggtctcc
      721 aggaccgtat ggcggaggtc cgtcttgccg atgaactttc cggtgttcac gtcgatgacc
      781 gtcagcgcct cggtgtggtc gatgatgagg taggctcccg actggagcca caccttccgc
      841 tccagggcgg cctctatctc cttttcgatg ccgtagaact cgaaaagtgg cgtggtgccg
      901 ccgtagagac tcacttcggg atggtccgtt ccggagaaga gggacaggct gttgttgacg
      961 ttttcgtatt cttccctgcc gtccaccacg atctccgaca cggcgtcggt gagctcgtcc
     1021 cggagaactc tcccgagaag gcccaggtcc cggtagagca ggcacggggc ggactggatc
     1081 ctcccgttgt gctcgatctc cctccagagc gcgaggagaa cctccacgtc gtgggcgaga
     1141 ctttcctcgt ccaccccctc ggcggctgtc cggacgatga tgccgtaatc ctctccccgc
     1201 agttcccggg cgacggtctt cagtctcctc cgctcgttct cgtcggtgat cctcttggaa
     1261 actcccgact cctggccgtg gggcacgagg accacgtacc gtcccggaag ggagatccgg
     1321 gaagtcaccc tggctccctt gttctttcgt gccgtcttgg tgacctggac gagaagctcc
     1381 tggttcggct gaagattgag cccccgggcg tcgttcaggt acaggaaggc gttccttccg
     1441 tcaccgaggt tgacgaagga ggcatggatg cccgggagga cactctccac ccgggccttg
     1501 taaatctccc cggctttctg gcgctcccac atccgctcca cgaacaggtc ggccaggcgg
     1561 ccgtcttcga tgatggcgac cctcgtctct tccggatcga tacagttggc cacaatcttc
     1621 cggttcacgg cgcccggctc cgcatgtctt tcactcacgg cgacgccctc ctcccgaaga
     1681 aaactgattt ttttc
//
LOCUS       DAOTHD010000518         1694 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_84530_length_1694_cov_2.647956, whole genome shotgun sequence.
ACCESSION   DAOTHD010000518 DAOTHD010000000
VERSION     DAOTHD010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84530_length_1694_cov_2.647956"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1597
                     /gene="atpD"
                     /locus_tag="P5219_08720"
     CDS             164..1597
                     /gene="atpD"
                     /locus_tag="P5219_08720"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRX26883.1"
                     /translation="MEHKPASSQKGIIQSVRGSVVDIRFEEDIPGIYTMLRTGNEGEV
                     VIEVLSQRSGNVVRGISLTPTQGLSRGMPVENTGRTLQAPVGKGILSRMFDVLGNTID
                     QEGPLPDVQWRSIHNDPPPLARRSTKSEIFETGIKAIDVLVPLERGGKSGLFGGAGVG
                     KTVLLTELIHNMVGREEGVSLFCGIGERSREGEELYREMKAAGVLANMVMVFGQMNEP
                     PGARFRVGHAALTMAEYFRDDEHRDVLLLIDNIFRFIQAGSEVSGLLGQMPSRLGYQP
                     TLGTELSKLEERIANTDTGAITSIQAVYVPADDFTDPAAVHTFSHLSASIVLSRKRAG
                     EGLYPAIDPLQSSSKMATPGIVGERHYLLAQEIRKTLAQYAQLRDIIAMLGIEQLSVE
                     DRNVVARARRIERFLTQPFFTTEQFTGLKGRLVSREDALTGCERILNDEFTEYPENAL
                     YMIGSVDEAKEKRKSIQTSGKSVPAPE"
BASE COUNT          371 a          487 c          486 g          350 t
ORIGIN      
        1 tatcatacat agtatttctc gacggccccg atatacggtt gtttcaaacc gttgcctgca
       61 gaaaaaatac tactcttttt acagctctat tctgaaccaa gcttgcagtt tccctgcaaa
      121 tccatcaggc gtatccggac gtcgcgccgg aaaggaaaac tcaatggagc acaagcctgc
      181 ttcatctcaa aaaggaatta ttcaatcagt acggggaagc gtggtggaca tccgctttga
      241 agaggacatt cccggcatct ataccatgct tcggaccgga aacgagggag aggtcgtcat
      301 cgaggtgctc tcccagcgaa gcgggaatgt cgttcggggc atttccctga cgcccacgca
      361 gggactctcc cggggaatgc ccgtagagaa tacgggcagg actcttcagg ctcctgtggg
      421 aaagggaatc ctttcccgga tgtttgatgt cctcggcaac accatcgatc aggaagggcc
      481 gcttccggac gttcagtggc gttcgattca caatgatccc ccacccctgg cccgccgttc
      541 gacaaagtcc gagattttcg agacggggat caaggccatc gacgtcctcg ttcccctgga
      601 gcgggggggg aaatccggtc tcttcggcgg tgccggagtc gggaagacgg tcctcctcac
      661 ggagctcatc cacaatatgg tcgggcggga ggaaggtgtc agcctcttct gcggaatcgg
      721 cgaacggagc agggagggag aggaactcta cagggagatg aaggcggcag gagttcttgc
      781 caacatggtg atggtcttcg ggcagatgaa cgagcccccc ggggcccggt tccgggtggg
      841 tcatgccgcc ctgaccatgg cggagtattt tcgggacgac gagcatcggg acgtgctgct
      901 cctcatagac aacattttcc gctttatcca ggcgggctca gaggtctcgg ggctgctcgg
      961 gcagatgccc tcccgcctgg gctatcagcc caccctcgga acggagcttt ccaagctcga
     1021 ggagcggatc gccaacaccg acacgggagc catcacctcg atccaggcgg tctatgttcc
     1081 ggcggacgac ttcaccgacc cggccgccgt ccacacgttc tcccatctct ctgcgtccat
     1141 tgttctctcc aggaagcggg cgggcgaggg gctgtacccc gccatcgacc ccctccagtc
     1201 aagttcgaag atggccactc ccggaatcgt gggtgagcgc cattatcttc ttgcccagga
     1261 gatacggaag acccttgccc agtatgccca gctcagggat atcatcgcca tgctcggcat
     1321 cgagcagctt tccgtcgagg accggaatgt tgtggcccgg gcccgccgga ttgagcgttt
     1381 cctcacccag cctttcttca ccaccgaaca gttcaccggt ttgaagggca ggctcgtgag
     1441 ccgggaggat gcgctgaccg gctgtgagcg tatactgaat gacgaattca cagaataccc
     1501 ggaaaatgcc ctctacatga tcggatccgt ggatgaggca aaagaaaaga ggaaatcaat
     1561 acaaacttcc gggaaaagtg ttcctgctcc tgaataaaga gaatctgtcc ggagttcttt
     1621 tcccccggac ggaaaggagg agcctatgaa gcagactgtc atgaccatga aaattcttct
     1681 tcccttccag gttt
//
LOCUS       DAOTHD010000519         1690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_84804_length_1690_cov_3.034862, whole genome shotgun sequence.
ACCESSION   DAOTHD010000519 DAOTHD010000000
VERSION     DAOTHD010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84804_length_1690_cov_3.034862"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="P5219_08725"
     CDS             <1..269
                     /locus_tag="P5219_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26884.1"
                     /translation="ARWRIAAEVIRSFKGGYVPGEALEYFRTTETEFPPPEKGSRHIV
                     SFVLEEDRPVIPDEGYHFPWSAELGEQVAAELARPAQLGENYPD"
     gene            complement(351..839)
                     /locus_tag="P5219_08730"
     CDS             complement(351..839)
                     /locus_tag="P5219_08730"
                     /inference="COORDINATES: protein motif:HMM:NF039481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HRX26885.1"
                     /translation="MNIPTTLKHKPVITVENYENVDGRQAYNSDAKGLSLGLAQWNDR
                     GKVEISAKVWRYTGEKWSRQSEEMPLHRVLDLAILVCKGKQHFQEAYRHPKLYDETNP
                     RIDRVGLQGDAMTVEICTGNPRIDEDIQLFSQALSRDDELLSERLHLLAELLRDMGYG
                     RG"
     gene            1086..>1690
                     /locus_tag="P5219_08735"
     CDS             1086..>1690
                     /locus_tag="P5219_08735"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRX26886.1"
                     /translation="MEAVLRTSSLVFLDRIVYPDMEVRKGEIIFLQGPSGSGKSSFLR
                     LLNGTVSPSGGTVLYGGKDILEIDPLLLRREVILAGQSAYLFPGTVRENFLQYHQYRD
                     SVPPDSESMQACLDACRVPFSLDASCDTLSGGEKQRVFLAIALSFRPAVFLLDEPTSA
                     LDGATAGALMANLTEYCRNSGVAAVIVSHDRHLTERFAERTV"
BASE COUNT          308 a          503 c          495 g          384 t
ORIGIN      
        1 acgcccgatg gagaattgcg gcggaagtga tccgctcctt caagggcggg tacgtcccgg
       61 gggaggccct ggaatatttc cggaccacgg agacggagtt tccgcccccg gaaaagggca
      121 gccgccacat cgtcagtttt gttctggagg aggaccgtcc cgtcatcccg gacgagggct
      181 accatttccc ctggtctgcg gagctcgggg agcaggtggc ggcggaactc gcccgccccg
      241 cgcagctggg ggagaactat cctgattgag cacggcgggg cggccaatac cttcgacacc
      301 ttccgggctg aggggttcat ccgggcggcg gagcagccgc ccggtttctt tcagcctctg
      361 ccgtacccca tgtcgcggag cagttcggcc agaaggtgaa gccgctccga cagcagctcg
      421 tcgtcccggc tgagggcctg ggagaagagc tggatatcct cgtcgatcct ggggttgccc
      481 gtgcatattt ccacggtcat ggcgtccccc tggagcccca cacggtcgat gcgggggttt
      541 gtttcatcgt agagtttcgg gtggcggtag gcctcctgga aatgctgctt ccccttgcac
      601 acaaggatgg ccaggtcgag cacccggtgg aggggcatct cctccgactg gcgtgaccat
      661 ttttcccccg tatagcgcca caccttggcg gaaatttcca cctttcccct gtcattccac
      721 tgggcaagcc cgagggacag ccccttggcg tcggaattgt acgcctgccg cccgtccacg
      781 ttctcgtagt tctccacggt gatgaccggc ttgtgcttca gcgtcgtcgg tatgttcatt
      841 ttttcctcct gaatatcctt tgataattac taaattactt atttactaaa taagtacgca
      901 gacaagcata ctccaattct ttttcctgtc aagctgttcc ctggaaaaaa tccgggggcc
      961 cccgcttttc cggtgacacc gctttcggtc cttttttcag gagggtatgt cccgaatggt
     1021 caggaaacat gagataatat tattccagaa tgcctgatat gcaggcagga aggcaaggtg
     1081 gagtcatgga agcagttctt cgcacgtcat ccctcgtctt tctggacagg attgtttatc
     1141 ctgacatgga agtgcgcaaa ggtgagatca tttttcttca ggggcccagc gggagcggga
     1201 aaagctcctt tctgcggctt ttgaacggta ccgtttcccc ttcgggcggc acggtgctgt
     1261 atgggggcaa agatatcctt gagatcgacc ctctccttct ccggagggag gtcatcctcg
     1321 ccggacagtc ggcctatctt ttccccggca cggtgaggga aaactttctt cagtaccacc
     1381 agtacaggga ttccgttcct ccggacagcg aatccatgca ggcgtgcctt gacgcctgcc
     1441 gggtcccctt ttcccttgac gcatcctgcg acacactctc cggcggcgag aagcagcgtg
     1501 tattccttgc catagcgctg agcttccgcc cggcggtgtt tttgctggac gagcccacct
     1561 cggccctcga cggagccact gcaggggcat tgatggccaa cctgacggag tactgcagaa
     1621 acagcggcgt cgctgccgtt atcgtgagcc acgacaggca cctgacggaa cggttcgccg
     1681 aacggaccgt
//
LOCUS       DAOTHD010000520         1689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_84865_length_1689_cov_3.298654, whole genome shotgun sequence.
ACCESSION   DAOTHD010000520 DAOTHD010000000
VERSION     DAOTHD010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84865_length_1689_cov_3.298654"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..1596)
                     /locus_tag="P5219_08740"
     CDS             complement(88..1596)
                     /locus_tag="P5219_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HRX26887.1"
                     /translation="MKQKGRLLVVSNRLPVTLSCEEGKWKGFPASGGLVSAMNPVLKN
                     RGGFWAGWPGTGSGARLADIRDILGPVSEESGYRFLPVMLTEDDVHDFYHGYSNSVLW
                     PLFHDLQSRCDFSPHYWDGYIRSNAKFARVVLRHAAEDDFIWVNDYQLIPLGAMLREK
                     QPGLRTSFFLHIPFPSVDVFMKMPRRREILDQMTRYSFVCFQTGRDRKNFLDCLKVFH
                     PECRVSGRGHIVKVHLGDRSFSLGSLPISIDYQAYSSLGGTRHAVARAKEIRQEIFGE
                     KFILGVDRLDYSKGIPEKLRGFRRCLELYPELRERITLHQVVVPSRETVEQYQALKEE
                     IELLISRINGEFSTTRWVPVNWHYGSVSREELAAMYRAADMALVTSLKDGMNLVCKEY
                     CACKGREKGVLILSEFAGAALQLARGAVLVNPYDVDAVAGAVRRAFFMDDREKKERMR
                     FLRKSIARQDVFWWVDNFLRAAAGKKLEDFPEQAIPSLWPGVADGEQNDPGL"
BASE COUNT          345 a          534 c          464 g          346 t
ORIGIN      
        1 cgaatcagcg tatttatatt gtagtctgtg ccccctgaga agtaaactcc cgcatgagcg
       61 cctcaatccg catacagcgg gaagcggcta caagcctggg tcgttttgct ccccgtccgc
      121 cacaccgggc cagagggagg gaatggcctg ctcgggaaaa tcttccagct tttttcctgc
      181 ggcagcccga agaaaattgt ccacccacca gaaaacatcc tgccgggcga tggacttcct
      241 gagaaaccgc atcctttcct ttttttccct gtcatccatg aagaaggctc tcctgaccgc
      301 ccctgcaacg gcgtccacgt cataggggtt caccaggacc gctccacggg caagctgaag
      361 ggctgctccg gcaaactcgc tgagaatgag gactcccttt tcccgcccct tgcaggcgca
      421 gtactccttg cagacaaggt tcattccgtc cttgagggac gtcacgaggg ccatgtccgc
      481 cgcccggtac atggccgcga gttcttccct tgacacggag ccgtaatgcc agttcaccgg
      541 aacccaccgg gtggtggaaa actctccgtt gatccggctg atgagcagct cgatttcctc
      601 cttgagggcc tggtactgct ccaccgtctc cctgctgggg accacgacct ggtgcagggt
      661 aatccgctcg cggagttcag gatacagttc caggcacctc cggaaccccc tgagcttttc
      721 cggtatgccc ttggaatagt ccagcctgtc gacgccgagg atgaactttt ccccgaagat
      781 ctcctgccgt atttctttcg ccctggccac tgcgtgcctt gttcccccca gggaggagta
      841 ggcctggtaa tcaatgctga taggaagact ccccagggag aacgagcggt cgccgagatg
      901 aactttcacg atgtgccccc ggccggaaac cctgcattcc gggtggaata ccttcaggca
      961 gtcgaggaaa ttcttcctgt ctctgccggt ctggaagcac acgaaggagt accgggtcat
     1021 ctggtcgagg atttccctgc gcctgggcat cttcatgaac acatcgacgc tggggaaagg
     1081 aatgtggaga aaaaaagacg tcctgaggcc cggctgcttc tcccggagca tggcccccag
     1141 ggggatgagc tggtaatcgt tcacccatat aaaatcatcc tccgcggcat gcctcaggac
     1201 aacccgggcg aacttcgcgt ttgagcgaat atagccgtcc cagtagtggg gggaaaaatc
     1261 gcaccggctc tggagatcgt gaaaaagagg ccacagcacg gagttggaat atccgtggta
     1321 aaagtcatgc acatcgtcct ccgtgagcat gactggaaga aagcggtacc cgctctcctc
     1381 cgacaccggg ccgaggatgt cccgaatgtc cgccaggcgg gcacctgaac cggttccggg
     1441 ccacccggcc cagaaaccgc ccctgttctt cagcaccggg ttcatggcgc tgaccagtcc
     1501 ccccgacgcc gggaagcctt tccattttcc ctcttcacag gagagggtca caggaaggcg
     1561 gttggacacc accagcagtc ttcctttttg tttcatggcg ttccccccct tccggctgta
     1621 agggcgccaa gccaccttcg aaggaattcc ggcacttcct ccggcaggat cctcacaccg
     1681 gcggcactg
//
LOCUS       DAOTHD010000521         1688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_84951_length_1688_cov_3.056950, whole genome shotgun sequence.
ACCESSION   DAOTHD010000521 DAOTHD010000000
VERSION     DAOTHD010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84951_length_1688_cov_3.056950"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="P5219_08745"
     CDS             <1..1084
                     /locus_tag="P5219_08745"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HRX26888.1"
                     /translation="WGRGVLDNKGPVIIGLFAAKAIFDAGLKLKRRVRIIFGTNEEQG
                     SKCMKRYVELGEDLPAAGFTPDAEYPIIHAEKGIVTYTVSLPFAPSGEMKITSLEGGV
                     AANVVMAEVTAVIEDSRAECRQRITAAASAWKGPEGSSLTADDDGSRVTLVMKGHPAH
                     GSTPEKGINALACLADFMAGLKLKGEQGVFFSAFSRLAGFETDGKSLGVAMADEVSGP
                     LTVCVGVVKAENGRASFTINMRYPVTAKEEAVTGPIEETFRKNGLTVEKVSKANPLYM
                     PPESRLIQALQRVYTEETGQEATLLAIGGGTYAKTMPNVVAFGPVFPGQDYTIHEEDE
                     RWSVEDIMKNAHIMAKVLVELAETKE"
     gene            complement(1161..>1688)
                     /locus_tag="P5219_08750"
     CDS             complement(1161..>1688)
                     /locus_tag="P5219_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRX26889.1"
                     /translation="RLVADRLSRRILGAQIMGSGVVDKRLDALVAAVSSGMTVDLLAD
                     GDFAYAPPFSTALDPNTHAANALKNKMDGLVKTYQPRELRERMQGANPPLLLDVRTPG
                     ELDLQGRLPYEFVNIPLGKLRERVGELPRDREIVAFCKISVRGWDALATLKGAGYEDV
                     ALLEGGVMAWPYELK"
BASE COUNT          327 a          530 c          540 g          291 t
ORIGIN      
        1 ctggggccgg ggcgtcctcg acaacaaagg accggtcatt atcggtcttt tcgccgcgaa
       61 ggccatcttc gacgccggtc tgaagctgaa gcgcagagtc cggatcatct tcggcaccaa
      121 cgaggagcag ggcagcaagt gcatgaaacg ctatgtggag ttgggggagg atctccccgc
      181 cgcggggttc acccctgacg ccgagtaccc catcattcat gcagaaaagg ggatcgtcac
      241 ctacacggtc tcccttccct tcgctccctc gggcgaaatg aagataacct ccctcgaggg
      301 aggcgtggcc gccaacgttg ttatggccga ggttacggcc gtgatcgagg attccagggc
      361 cgagtgcagg cagcgcatca ccgctgccgc ctccgcatgg aaggggcccg agggcagctc
      421 ccttaccgca gatgatgacg gcagcagggt caccctggtg atgaagggcc accccgccca
      481 cggaagcaca cccgaaaagg gaatcaatgc cctcgcctgc ctggcggact tcatggcggg
      541 gctgaagctg aagggcgagc agggggtgtt tttctccgcc ttctcccggc tcgcgggttt
      601 cgagaccgac gggaaatccc tgggcgtcgc catggccgac gaagtgtccg ggccgctgac
      661 ggtctgcgtg ggcgtggtga aggctgagaa tggcagggct tctttcacca tcaacatgcg
      721 gtaccccgtc acggcgaagg aagaggctgt cacgggcccc atcgaggaga ccttcaggaa
      781 gaacggcctg acggtggaga aggtgtccaa ggcgaacccc ctgtacatgc ctcctgaatc
      841 caggctcatt caggccctcc agagggtcta cactgaagaa acaggccagg aagccacgct
      901 gctggccatc ggcgggggca cctatgccaa gaccatgccc aacgtggtgg ccttcggccc
      961 cgtcttcccc gggcaggatt acacaatcca cgaggaagac gaacggtggt ccgtggagga
     1021 catcatgaag aacgcccaca tcatggcgaa ggtcctggtg gagcttgccg agacaaagga
     1081 atagaccggg agaaacagaa aggggctgcc gcagggatcc ctgcggcagc ccctttctgt
     1141 ttctcctgtg gtccggagga ttatttcagc tcgtagggcc aggccatgac gcctccctca
     1201 aggagagcca cgtcctcgta gcctgcacct ttcagggtcg ccagggcatc ccagccccgg
     1261 acggagattt tgcagaaggc cacgatctcc ctgtcccggg gcagttcacc cacccgttcc
     1321 cggagcttcc cgagagggat gttcacgaac tcgtagggga gacggccctg gaggtccagc
     1381 tctcccggcg tcctcacgtc cagcagcagc ggaggattcg ccccctgcat cctttccctg
     1441 agttcccttg gctggtacgt tttcaccagg ccgtccatct tgttcttcag cgcgttggcg
     1501 gcgtgggtat tcgggtcgag ggccgtggag aagggcgggg cgtaggcgaa gtcgccgtcc
     1561 gccaggagat ccaccgtcat cccggaggag acggccgcca cgagggcgtc aagacgcttg
     1621 tccaccaccc ctgaacccat gatctgggcg cccaggatgc gtcggctcag cctgtcggcc
     1681 accagccg
//
LOCUS       DAOTHD010000522         1683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_85312_length_1683_cov_3.412162, whole genome shotgun sequence.
ACCESSION   DAOTHD010000522 DAOTHD010000000
VERSION     DAOTHD010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85312_length_1683_cov_3.412162"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /gene="pyrH"
                     /locus_tag="P5219_08755"
     CDS             <1..678
                     /gene="pyrH"
                     /locus_tag="P5219_08755"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRX26890.1"
                     /translation="SNGFGFDFDAVRRIGEEIVEVARSGIQIAMVVGGGNMLRGRELE
                     KLGVERAQADYMGMLATVMNALCLQDVLEKFGVPTRVQTAIEMRQMAEPYIRRRAIRH
                     LEKGRIVIFAAGTGSPYFSTDTAAALRAAEMEADCLLKATKVDGIYNADPKKHPDAVL
                     LHRLTYMEALRRQFEVMDAAAFSLCMENAIPIVVLNILQKGNLKNFLVKGTDTGTIVS
                     AQEDGGA"
     gene            734..1291
                     /gene="frr"
                     /locus_tag="P5219_08760"
     CDS             734..1291
                     /gene="frr"
                     /locus_tag="P5219_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRX26891.1"
                     /translation="MPKEELKVLRERMEKVIEHLKGEFLAIRTGRAHPGLVSDIKVDY
                     YGAPTPIKQIATISVPEGRQIAISPFDRSALKLVEKAILASSLGVTPQNDGEIIRVNL
                     PELTRERRVELTKLVGKYAEESRIALRNLRRDSNEVLKKKEKNSEISEDDLKKYTKDV
                     QDVTDEFIKKVDETLKAKEKEIMEE"
     gene            1359..>1683
                     /locus_tag="P5219_08765"
     CDS             1359..>1683
                     /locus_tag="P5219_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HRX26892.1"
                     /translation="MTLPKAYVTRRIQDAGLNLLPGRVDFEVWEKTSPVEREVLLEKA
                     LEVEGLLCTLSDRIDEELLSRSPGLKVVSNYAVGYDNIDVDAATRRGVLVTNTPDVLT
                     NATADI"
BASE COUNT          434 a          421 c          502 g          326 t
ORIGIN      
        1 tccaacggat tcggatttga tttcgacgcc gtgcgccgca taggggaaga gatagtcgag
       61 gtggcccggt cgggaatcca gatcgccatg gtcgtcgggg gcggtaatat gctccggggc
      121 cgtgaactgg aaaagctcgg agttgaacgg gcccaggcgg actatatggg aatgctcgcc
      181 acagtgatga atgccctctg tctccaggat gtccttgaga aattcggcgt tcccaccagg
      241 gtccagaccg ccattgaaat gcgccagatg gccgagccgt atatccggcg ccgtgccatt
      301 cgccaccttg aaaaggggag aatcgtaatt tttgccgctg ggacggggtc cccctatttt
      361 tctaccgata cggccgcagc gctgcgggct gccgaaatgg aagcggattg cctgttgaaa
      421 gccaccaagg tagatggtat atataatgcg gaccccaaga aacaccctga cgcggtgctt
      481 ctgcacaggt tgacctacat ggaggcgctt cgccggcagt tcgaggtgat ggacgcggca
      541 gctttctccc tttgcatgga aaacgccata ccgatagtgg tattgaacat actccagaaa
      601 ggaaacctga aaaactttct ggtgaagggt accgacacgg gaaccatagt atccgcacaa
      661 gaggacggcg gggcctgaaa aagcgggacg ggcgtccgcc tttccattct tcatcaagag
      721 gaggaattcc ggtatgccga aagaagagct gaaagttctg cgggaacgca tggaaaaggt
      781 cattgaacac ctgaagggcg aatttctggc catcaggacg ggacgggctc atccggggct
      841 ggtgagtgac atcaaggtgg attattacgg agcccccact cccatcaagc agattgccac
      901 catctcggtt cctgagggaa ggcagattgc aatttctccc ttcgacaggt ccgctctcaa
      961 gcttgtcgag aaggccatac tggcttcatc ccttggggtt acgccccaga atgacgggga
     1021 aattatccgg gtgaacctgc ctgaactcac cagggaacgg cgtgtggaac tgacgaagct
     1081 cgtgggcaaa tacgcggagg aaagcaggat tgccctgagg aaccttcggc gggattcgaa
     1141 tgaagtcctc aaaaagaagg aaaagaactc tgaaatcagc gaagacgacc tgaaaaaata
     1201 cacgaaggac gttcaggacg taaccgacga attcatcaaa aaagtcgacg aaacgctgaa
     1261 ggccaaggaa aaggagatca tggaggagta aacaggtact ccggattttt caggaagcgg
     1321 ggggagtacc cccgcttctt ttttaaggag ggatggacat gacgctgccg aaagcgtacg
     1381 taacccgcag gattcaggac gcggggctca accttcttcc cggccgggtt gattttgaag
     1441 tgtgggaaaa aacctcccct gtggagcggg aagtgcttct tgaaaaagcc ttggaagttg
     1501 aagggctcct gtgcaccctt tcggacagga tagatgaaga gcttctctcc cgctcaccgg
     1561 ggctgaaagt ggtgagcaac tacgccgtgg gatacgacaa catcgacgtt gatgcagcca
     1621 caagaagagg agtcctggtc accaacactc ccgacgtgct gaccaatgca accgccgata
     1681 tcg
//
LOCUS       DAOTHD010000523         1682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_85437_length_1682_cov_2.016595, whole genome shotgun sequence.
ACCESSION   DAOTHD010000523 DAOTHD010000000
VERSION     DAOTHD010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85437_length_1682_cov_2.016595"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="P5219_08770"
     CDS             <1..1166
                     /locus_tag="P5219_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRX26893.1"
                     /translation="SSHAAVLKVRPELKWPADMYLEGSDQHRGWFQTSLLTSVGMYGR
                     PPYEQVLTHGFIVDGDGKKMSKSLGNVVAPQEVINEYGADILRLWVASTDYRNDIRIS
                     QGIIKNLSESYRRIRNTARYLLANLKGFDPEHHAVPVEKMTEMDRWILQKLNGVIERA
                     NEAYDDYEFHVPTFAIHQFCVNELSSFYLDSSKDRMYADGTDSLSRRSGQTAMWLVLS
                     AVIRMLAPVLSFTAEEVWQEMRKIDAKLPESIFLSDWPAPFAVADGKKLEEKWAKVLS
                     VRAAVSRVLEQARSAGIIGHSLDARVEVERKGAPEAADLFTDEDLRVFSIVSGFRWVE
                     RIDDMAVVRDDEETGLRIGVDKARGEKCPRCWQYTEEGDENGLCPRCSEVLSA"
     gene            1185..>1682
                     /locus_tag="P5219_08775"
     CDS             1185..>1682
                     /locus_tag="P5219_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HRX26894.1"
                     /translation="MMEDLPPVGSSSCEDDDGGFPDSPENGTETIAAGEEDRGIRLDV
                     FLSSRLGITRTFARKLVEDGNVSLLPHRKAKPGMKMVPGISAEVTLPPPESLDLEPEE
                     VPFGVVYEDEWLIVIDKPSGVVVHPAPGNRHGTLVHGLLHRFRHFGSFNNVLRPGIVH
                     RLDAST"
BASE COUNT          365 a          480 c          517 g          320 t
ORIGIN      
        1 gatcaagcca cgccgccgtg ctgaaggtca ggccggagct gaaatggccc gccgacatgt
       61 atcttgaggg aagcgaccag caccggggct ggttccagac gtccctcctc acctccgtgg
      121 gaatgtacgg aaggcccccc tatgagcagg tgcttaccca cggattcata gttgacggcg
      181 acggcaagaa gatgtcgaaa tccctgggga acgtggttgc cccccaggag gtaatcaacg
      241 agtacggtgc ggacatcctg aggctgtggg tggcttccac ggactaccgg aacgatatcc
      301 gcatctccca gggcatcata aagaacctga gcgaatcata ccggagaatc cggaataccg
      361 cccggtatct cctcgccaac ctgaagggat ttgacccgga gcatcatgcg gttcccgtgg
      421 aaaaaatgac ggagatggac cggtggatcc ttcagaagct caacggggtc atcgaaaggg
      481 cgaacgaagc ctacgacgac tacgagttcc acgtgccgac ctttgccata caccagttct
      541 gcgtgaacga actcagctcc ttctacctgg attcgagcaa ggacaggatg tacgccgacg
      601 gcaccgattc actgtcgagg agaagcggcc agacggccat gtggcttgtc ctttcggcag
      661 ttatccggat gctggccccg gtcctcagct tcaccgccga ggaagtctgg caggaaatgc
      721 gaaagatcga tgcgaagctc ccggaaagta ttttcctttc agactggccc gcaccctttg
      781 ccgtggccga tgggaagaag cttgaagaga aatgggcgaa ggtcctctcc gtgcgggcgg
      841 ccgtgagtcg ggtcctcgag caggcgagaa gcgccggaat catcggccac tcgctggacg
      901 ccagggtcga ggtggagcgg aaaggcgctc ccgaggcggc cgatcttttc acagacgaag
      961 accttcgggt cttttccatc gtctcgggct tccgttgggt tgagaggatc gacgacatgg
     1021 cggtggtgcg tgacgatgaa gaaacaggtc tccggatcgg agtggacaag gccaggggcg
     1081 agaagtgccc caggtgctgg cagtataccg aggaaggcga cgaaaacggg ctctgtccaa
     1141 ggtgctcgga agtactttcc gcctgagcct gccggtcgtt cttcatgatg gaggatcttc
     1201 cccccgtcgg ttcttcttcc tgtgaagacg acgacggggg gttccccgac agtcccgaaa
     1261 acggaacgga aaccattgct gccggagagg aagacagggg catccgcctc gatgtctttc
     1321 tttcctccag gctggggatt acccgaacct tcgcgagaaa actcgtggaa gacggaaatg
     1381 tcagccttct ccctcacagg aaagccaaac ccggaatgaa aatggtcccg ggaatatcgg
     1441 cggaggtgac gcttcctcct ccggagtctc tcgatctgga gccggaggag gtccctttcg
     1501 gcgtagtcta cgaagacgaa tggctgatcg tgattgacaa gccgagcggc gtggtggttc
     1561 accccgctcc gggtaaccgt cacggaaccc ttgtgcatgg tctgctccat cgtttccgcc
     1621 atttcggttc cttcaacaac gtcctccgcc cggggatagt gcaccggctc gacgcatcca
     1681 ca
//
LOCUS       DAOTHD010000524         1678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_85699_length_1678_cov_3.004313, whole genome shotgun sequence.
ACCESSION   DAOTHD010000524 DAOTHD010000000
VERSION     DAOTHD010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85699_length_1678_cov_3.004313"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="P5219_08780"
     CDS             <1..245
                     /locus_tag="P5219_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HRX26895.1"
                     /translation="FPPGSLAENLVAEGLPEELPVGARLAVGSSVLLEVVEKGKKPGE
                     PHSYDYRGWCLLPSAGYFLRVVRGGEVRKGDPVTME"
     gene            352..>1678
                     /locus_tag="P5219_08785"
     CDS             352..>1678
                     /locus_tag="P5219_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRX26896.1"
                     /translation="MIRNFKIGAKLALAFGVLLLIFAGVGALSWFNMGEVSREARSLA
                     DEYVPEMVVANRVQSTVQELMYEIRGYGYTYDRTFLEAGRRAAESARAALGEAAALAG
                     KYPALLTLRADAAKALSGLDEYVKLMDDTEKTVEIMNALRNRGTDGERAFFENAEKYV
                     QSQEQALEREFGESTLQSELSDRLQKIVLGNALIGLGNLVRVANYRGQAQRNPVLLEE
                     GLQHFSEVESTLERLKAMTFQRVNIEQLDAVMKAGTEYRSVMAEILAAWRRLDEINAR
                     RVETGRSILALADEVVKAGAANSQKIADHAVGSLASTISVILLSTAAAVLLGAAIAFA
                     MTRSLTRPLNRVTVLAGMAKEGDLSIEREDFRIVTRDELGNMADALADMVRGQREMVR
                     ELKEKSVHLSALSEETAASTEEVTSTTNEVAESNAHLAEQTRKGRENSIE"
BASE COUNT          349 a          470 c          579 g          280 t
ORIGIN      
        1 ccttccctcc gggatccctg gccgagaacc tggtggcgga agggcttccc gaagaactgc
       61 ccgtgggtgc ccgcctggcg gtggggagtt ctgtcctcct tgaggttgtg gagaagggga
      121 aaaaacccgg ggaaccccat tcctacgatt accggggctg gtgccttctg ccctcggcgg
      181 ggtactttct ccgggtggtc cggggcggag aggtacgaaa gggagatccg gtcacgatgg
      241 agtgacccgg agaaaggggg gtgtccgccg gtgcgccatg tttcccgtgg cgggcagccg
      301 ccgtgaaaga gctttttggg tacatattct caagaggaga gaggtggaag aatgatcagg
      361 aatttcaaaa taggggcgaa gcttgccctg gctttcggcg tcctgctgct cattttcgcc
      421 ggggtggggg cgctgagctg gttcaacatg ggggaagtga gccgggaagc ccgttccctc
      481 gccgacgagt atgttccgga aatggtggtg gccaacaggg ttcagagcac agtgcaggaa
      541 ctcatgtatg aaatacgggg ctatggctac acctacgacc ggacctttct cgaagcgggc
      601 cgccgggccg ctgaaagtgc ccgggcagcc ctgggggagg cggctgccct cgccgggaag
      661 taccccgccc tgctcaccct cagggcggac gccgccaagg ccctgtcagg cctggacgag
      721 tacgtgaagc tcatggacga taccgaaaag accgtggaga tcatgaacgc actcaggaac
      781 aggggaactg acggcgagag ggcctttttc gaaaacgcgg agaagtacgt tcagtcccag
      841 gaacaggccc tcgagaggga gttcggcgag agcacccttc agtcggagct ttcggaccgg
      901 ctgcagaaga tcgttctcgg caacgctctc atcggtctgg ggaacctcgt gcgggtggcc
      961 aactacaggg gacaggccca gaggaacccc gtgctccttg aagagggact ccagcacttc
     1021 agtgaagtgg agtccaccct ggagcgcctg aaggccatga ctttccagag agtgaacatc
     1081 gaacagctcg acgcggtgat gaaagccgga acagaatacc ggtccgtcat ggcggaaata
     1141 ctggccgcct ggagacggct tgacgaaatc aacgcccgga gggttgagac gggaagaagc
     1201 attctcgcgc tggccgacga ggttgtgaag gcgggggcgg cgaattccca gaaaatcgcc
     1261 gatcatgccg tcggcagcct ggcgtccacc atttccgtca ttcttctctc caccgcagcg
     1321 gcggtgctgc tcggcgctgc catagccttc gccatgaccc ggagcctcac ccgtcccctg
     1381 aacagggtga ccgtcctggc cgggatggcg aaggaagggg atctctccat cgagcgggag
     1441 gatttccgga tcgtcacccg ggatgaactg ggaaacatgg cggatgccct ggcggacatg
     1501 gtccgggggc agcgggagat ggtccgggag ctgaaggaaa agtcggttca cctgtcggcc
     1561 ctgtccgagg aaacggccgc gtccacggaa gaggtcacca gcaccaccaa cgaggtggcc
     1621 gagagcaacg cccacctggc cgagcagacc aggaagggcc gggaaaattc catcgagg
//
LOCUS       DAOTHD010000525         1676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_85823_length_1676_cov_2.927205, whole genome shotgun sequence.
ACCESSION   DAOTHD010000525 DAOTHD010000000
VERSION     DAOTHD010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85823_length_1676_cov_2.927205"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="P5219_08790"
     CDS             complement(<1..225)
                     /locus_tag="P5219_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRX26897.1"
                     /translation="MVKVSGGIRVREAQCRGCANCIKSCPTEAIRVIDGLIRIIPDLC
                     IDCGECIRSCRDKAITVNEDEWDLLRSREGL"
     gene            complement(275..709)
                     /locus_tag="P5219_08795"
     CDS             complement(275..709)
                     /locus_tag="P5219_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX26898.1"
                     /translation="MGQEPYVEEYTIQAGDFTAIGEASTKFRATLKMLGIPSEVVRRA
                     AVVAYEAEMNAMIHAGGGTIRMTVAPDHLEILASDQGPGIPDVALAMQEGYSTASDEI
                     RELGFGAGMGLPNIERNSDGMNIETEVGVGTVLTARINFPGQ"
     gene            complement(713..1078)
                     /locus_tag="P5219_08800"
     CDS             complement(713..1078)
                     /locus_tag="P5219_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HRX26899.1"
                     /translation="MKLRDVVQNIRAEVLYGEELLDSIDIECAYGADLMSDVLAFARP
                     GSLLLTGLTNIQIVRTAQMLDLPAVVFVRGKKPQEAAVKLASQIKMPVLLCQMSMFET
                     CGILFAKGILPCHIPDRGV"
     gene            complement(1120..>1676)
                     /locus_tag="P5219_08805"
     CDS             complement(1120..>1676)
                     /locus_tag="P5219_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26900.1"
                     /translation="FWLAGAGPDSFRMIEDEAISDGVSVLVSWAGSMVVRDIWRAVRR
                     KEGAFRIWYGGEPGRILLSFNGVLAADQEVPLERDRSFVTLGREIWRRTRAVVRDASA
                     AGRAYAACGWIFEGFRRAGSPEPELLAKAMESAADFALGSFPLTVNPATHRPFAREVA
                     ILEVKDRAFRPIDFFTVTGPGYLP"
BASE COUNT          359 a          546 c          427 g          344 t
ORIGIN      
        1 cagcccttcc ctggaacgga gaagatccca ttcatcctcg ttcacggtga tggctttgtc
       61 cctgcagctt cgtatgcatt ccccgcagtc aatgcagaga tccgggatga tgcgtatcag
      121 cccgtcgatt acccggatcg cctcggtagg gcaggatttg atgcagttgg cgcaccccct
      181 gcactgggcc tcacgaaccc tgattcctcc ggacaccttc accactcctt cgtgtcattc
      241 cagtactgaa tgccgttcat gacgggtgga aagctcactg tcccggaaag ttgattctcg
      301 ctgtgagaac cgtgccgacc cccacttccg tctcaatgtt catcccgtct gaattccgtt
      361 caatattcgg cagtcccatg cccgctccga aaccaagctc ccggatctcg tcggaagcgg
      421 tggagtatcc ttcctgcatg gccagggcca cgtcgggaat acccggcccc tggtccgaag
      481 ccaaaatttc gaggtgatcc ggagctactg tcatgcggat tgttcccccg cccgcatgaa
      541 tcatggcgtt catctcggcc tcataggcca ccaccgccgc cctgcggacc acttccgacg
      601 gaattccgag catcttgagg gtcgcccgaa atttcgtaga ggcttcgccg atagcggtaa
      661 aatcccccgc ctggatggtg tactcttcga cgtacggctc ctgccccacg ctctacaccc
      721 ctctgtctgg aatatgacag ggcagaattc ctttcgcgaa cagaatgccg cacgtctcga
      781 acatgctcat ctggcacaga agcacaggca tcttgatctg ggatgcaagc ttcaccgccg
      841 cctcctgggg cttctttccg cggacgaaga ccacagcggg aagatcaagc atctgggcgg
      901 tcctgacgat ctggatgttc gtcagcccgg tgaggagtag ggatcccgga cgggcgaagg
      961 ccagcacatc gctcatgagg tcggcgccat aagcgcactc gatgtcgatg gagtcaagaa
     1021 gttcctctcc gtacagcact tccgcccgta tgttctgcac cacgtcccgc aatttcatat
     1081 cgtcatcctc ctcaatggta aaaacagcga caattatttt cagggtaaat atcccgggcc
     1141 cgtaaccgta aaaaaatcaa tcggcctgaa ggcccggtcc ttcacctcga gaatggcgac
     1201 ttccctggca aagggccggt gagtagcagg gttgacagta aggggaaaag agccgagggc
     1261 aaaatccgcg gctgactcca tggctttcgc caggagttcc ggttcaggtg aacccgccct
     1321 tcggaaaccc tcaaaaatcc acccgcaggc agcgtacgcc ctgccggcgg cgcttgcatc
     1381 cctgacgaca gccctggtgc gcctccaaat ttcccgcccc agggtgacaa aggacctgtc
     1441 cctctccagg ggaacctcct gatctgcggc cagaactccg ttaaaggaaa gaaggatccg
     1501 gccgggctcc cctccgtacc agatccgaaa ggctccttct tttctccgga cagctctcca
     1561 gatatcccgg acaaccatgg atcctgccca tgacacaaga acggacacac catcggatat
     1621 ggcctcgtcc tcgatcatcc tgaaagaatc cggtcccgcc cccgccagcc agaaat
//
LOCUS       DAOTHD010000526         1673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_86023_length_1673_cov_3.344870, whole genome shotgun sequence.
ACCESSION   DAOTHD010000526 DAOTHD010000000
VERSION     DAOTHD010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86023_length_1673_cov_3.344870"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="P5219_08810"
     CDS             <1..483
                     /locus_tag="P5219_08810"
                     /inference="COORDINATES: protein motif:HMM:NF038085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_6728 family protein"
                     /protein_id="HRX26901.1"
                     /translation="DFFRSAEVLDVRRLGKQRVEALQLLNALSPEYALRGWRNHPART
                     MWSGHSSALAFYGVVVCRRWTGLGYRDTCRDKILGALNIMGGDGDEDRLEQLYREALA
                     GKGRNFLPPWFGDETFHRSHRSNLLRKDPEWYGRFGWDDGPDLPYVWPLPAAEEMKRS
                     "
     gene            490..1485
                     /locus_tag="P5219_08815"
     CDS             490..1485
                     /locus_tag="P5219_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HRX26902.1"
                     /translation="MKPFTDPAYAGIATFLRAPAITPDDLREGDIAIAGIPFDTTCGS
                     RQGARFGPRGIREGSLHFVYQMDALNKGDKTMINAATGETVRFPFRNILRDLGDFTVY
                     PADVERTSASVRDGVEDIVLRGAFPVLLGGDHYVTFPAVEGFERAWKKKKNAPKLGYI
                     HIDSHLDLTDDTEAWGRLYHGSTARRVSELECFSPSNMVWIGASGGFVSRDRWDFLQN
                     SGTVQFSMADIESLGVGEVVRRAAAIASAGCDSLYVTIDIDVVDHAFAPGTGSYVYGG
                     ITSFQFLQLMDALRTVPAGAVDLVEVAPPLDPTGTTSRLAATGLIRLLLPRLSGQ"
     regulatory      1516..1623
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT          310 a          522 c          530 g          311 t
ORIGIN      
        1 gattttttcc gctccgcgga ggtgctggac gtccggagac tcggcaagca gcgggtggag
       61 gccctccagc ttctgaacgc actctctccg gagtacgccc tccggggatg gcggaaccat
      121 cccgcccgga ccatgtggag cggccactcc tcggccctgg ccttctacgg cgtggtggtc
      181 tgccgccgat ggaccgggct gggctaccgg gatacctgca gggacaaaat tctgggcgca
      241 ttgaatatca tgggcggcga cggcgacgag gaccggctgg agcagctcta ccgggaggcc
      301 ctcgccggaa agggacggaa tttcctgccc ccctggttcg gcgacgagac cttccaccgg
      361 tcccaccggt cgaatcttct ccgaaaggat cccgaatggt acggccggtt cggctgggat
      421 gatggcccgg acctgcccta cgtatggccc ctcccggcgg ctgaagaaat gaaaaggagt
      481 tgactgaaag tgaaaccctt taccgacccg gcctatgccg gcattgccac gttcctgaga
      541 gcccccgcaa tcaccccgga cgacctgcgg gagggggata tcgccatcgc aggtattccc
      601 ttcgacacca cctgcggctc ccgccagggg gcccgtttcg ggccgagggg catccgcgag
      661 ggaagcctgc acttcgtcta ccagatggac gccctgaaca agggggacaa gaccatgatc
      721 aacgccgcca cgggagagac ggtccggttc cctttcagga acatcctccg ggatcttggg
      781 gacttcaccg tgtatcccgc cgacgtggag cgcacctccg cttccgtgag ggacggagtt
      841 gaggatatag tcctgagggg cgcctttccc gtgctgctcg gcggcgatca ttacgtcacc
      901 tttccggccg tcgaggggtt cgagcgggcg tggaagaaga aaaaaaacgc tccaaaactg
      961 ggctacatcc acatcgattc ccacctcgac ctgaccgatg acacggaggc ctggggcagg
     1021 ctgtaccacg gctccacggc gaggcgggtg agcgagctcg agtgcttctc gccttccaac
     1081 atggtctgga tcggcgcgag cggaggcttc gtcagcaggg acaggtggga tttcctgcag
     1141 aattcgggga ccgtccagtt ctccatggcc gatatcgaat ccctgggcgt cggcgaagtg
     1201 gtccgccggg cggcggccat cgcttccgca ggatgcgatt cactctacgt caccatcgat
     1261 atcgacgtgg tggaccatgc ttttgccccg gggacgggat cctatgtcta cggaggcatt
     1321 acctccttcc agttccttca gcttatggat gccctccgca ctgtgcccgc cggagccgtt
     1381 gacctggtgg aagtcgctcc tcccctggac ccgacgggaa caacgtcacg ccttgcggcc
     1441 accgggctca tccggcttct tcttccccgg ctctcgggac aatgaccgga aaggaagagt
     1501 ggagtataat aggcttattc gcatcggggg agtccggaaa ggactgagag ggggcatcac
     1561 cgccccgacc cctggaacct gatccgggtc atgccggcga agggaaggtg cccgcttcaa
     1621 ggacattcct ccgatgcaaa tctgcaaagg aggatttttt tatggccggt gat
//
LOCUS       DAOTHD010000527         1673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_86035_length_1673_cov_2.855995, whole genome shotgun sequence.
ACCESSION   DAOTHD010000527 DAOTHD010000000
VERSION     DAOTHD010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86035_length_1673_cov_2.855995"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1673)
                     /locus_tag="P5219_08820"
     CDS             complement(<1..>1673)
                     /locus_tag="P5219_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26903.1"
                     /translation="DPRDFAGLDRAILSLGERASALIRDNVSKGIDARLKLIGDARTA
                     AEKDGERRKLLSELEETFKAYVEKGVDFPPKAHETMIALADALKVTVLNIPASELERM
                     KRYLDLAAEKPGSLDLVLGMAWRLFEGAYTTADGAIDAFNNWFDYLDNHTVARLRAMN
                     PDVFLPGPGGKTRAVTIPIGRINDRLNASILGAAGNSLPFKAVNLGQELYAYVSGFRE
                     GKDMSESVSNVATQLFRSRVPGGDIVEAVVMENYTRAAIGVVYLLFPTLAVPEALYGM
                     GTAAAEWYVGTWQQWQYDEMVDELYEGTAFEPTREGTWRVKSITYSLPGGGAVTVGRD
                     EAFTLPELVPKISNILVPQVRTHPSIAMYHELLARKELSDGQSGIPVWPRRYLNLSLY
                     GERLYEKYVSEVKTVTRQYFADVIAELEKRKAFDQGTNEKKLREIGEELGCGGSLLEK
                     TGSGREPQEAVIADWEEWLKVHGALMEFRERWSAFFILVRKPSCSPESIRIVLEEDRK
                     DLGEAKKALREAAVAVEEIVGRNGADEKTLQPAVRTRAGALIYPSGTPE"
BASE COUNT          261 a          558 c          522 g          332 t
ORIGIN      
        1 ctccggagta ccggaggggt agatcagggc ccccgccctg gtgcggacgg cgggctgcag
       61 ggtcttttca tcggccccgt tccggccgac gatctcctcc acggcaacag ccgcctcccg
      121 gagggccttt ttcgcctccc cgaggtcctt ccggtcctct tcgaggacga tccggatcga
      181 ttcgggagag caggagggct tccgcaccag gatgaaaaag gcgctccacc gctcccggaa
      241 ctccataagg gcgccatgga ccttcagcca ctcctcccag tcggcgataa ccgcctcctg
      301 gggttcccgg ccgcttccgg tcttttcgag aagagagccg ccgcagccca gttcctcacc
      361 tatctcccgc agtttctttt cgttcgtccc ctggtcgaag gccttccgct tttccagctc
      421 ggcgatgacg tcggcgaagt actgcctggt caccgtcttc acctccgaaa cgtacttctc
      481 atacagcctc tccccgtaca ggctgaggtt caggtatctc cggggccaga cggggatgcc
      541 gctctgcccg tcggagagct ccttccgtgc caggagttcg tggtacatgg cgatggacgg
      601 gtgggtcctc acctggggca cgagaatgtt ggagatcttc ggcaccagct ccggcagggt
      661 aaaggcctcg tcccgcccca cggtgaccgc tcctccgccg ggaaggctgt acgttatgga
      721 cttcaccctc cacgttccct cccgtgtcgg ctcgaaggcc gttccctcgt acagttcatc
      781 caccatttcg tcgtactgcc actgctgcca cgtgcccacg taccactcgg cggcggcggt
      841 tcccatgccg tagagggcct cggggacggc gagcgtgggg aagagaaggt agaccacgcc
      901 gatggccgcc cgggtgtagt tctccatgac cacggcttca acgatatctc ctcccggaac
      961 ccgggaccgg aagagctggg ttgccacgtt ggacaccgat tcggacatgt ccttcccttc
     1021 ccggaagccg cttacgtagg cgtagagctc ctggcccagg ttgacggcct tgaagggaag
     1081 ggagttcccc gcggcgccga ggatggacgc gttgagccgg tcgttgatcc tgccgatggg
     1141 tatggttaca gcccgggtct ttcccccggg gccgggaaga aacacgtccg ggttcatggc
     1201 ccggagacgg gccaccgtgt ggttgtcgag gtagtcaaac cagttgttga aggcgtcgat
     1261 cgccccgtcg gcggtggtgt aggctccctc gaagaggcgc catgccatgc cgaggaccag
     1321 gtcgagggaa ccgggctttt cggcggccag gtccaggtag cgtttcatcc gctcaagctc
     1381 cgacgccgga atgttgagca ccgtcacctt cagtgcgtcg gcaagggcga tcatggtctc
     1441 gtgggccttc ggggggaagt ccactccctt ctccacgtag gccttgaagg tctcctcgag
     1501 ctccgagagc agtttcctcc gctccccgtc tttttccgcg gctgttcggg catcgccgat
     1561 gagcttcagc cgggcgtcta tgcccttcga cacgttgtcc cggatcaggg cggaagcccg
     1621 ctcccccagg gagaggatcg cccggtcgag gccggcaaag tcccggggat ccc
//
LOCUS       DAOTHD010000528         1672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_86099_length_1672_cov_3.191095, whole genome shotgun sequence.
ACCESSION   DAOTHD010000528 DAOTHD010000000
VERSION     DAOTHD010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86099_length_1672_cov_3.191095"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="P5219_08825"
     CDS             <1..326
                     /locus_tag="P5219_08825"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRX26904.1"
                     /translation="RAAEEFPLAMKRCLPGCSSFLKEGPLLRIRTMRSRWGSCTGKGV
                     VTLNTLLVKASPACIDYVLIHELCHTVHHDHGKGFYSLLESILPGWKDRKRELLEQAK
                     CRHFI"
     gene            454..>1672
                     /locus_tag="P5219_08830"
     CDS             454..>1672
                     /locus_tag="P5219_08830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRX26905.1"
                     /translation="MSQTPSGQPGDKAGNSLEEQSALLEQLFNNAPLPIQRCSIEGQI
                     LRANQASEEVFGYTCEEMRGRFVDDLFAAPDTYDEAVKNTRDVLEGKTVFFEGRRRRK
                     NGEAIYVKGTAFPIVLKGVQIGTYSIYTDQTAVKDAEIRLSQSEERYRSLVDDIPAMI
                     CAFDDRGILSFVNREFCTFFSLPAENLLGTSLLRFIPPEHHPAIVSHLISLSETKPVI
                     TTEIPTLPPGSAPRWHRWTVRLVSERENGRRLFRAAGQDITDSRLVQTTLAENLAAME
                     RTFSEAIEVLASATDAKDPYTAEHQRRVARLSAAMAEQLGYDSAFIRGVEQAAMVHDI
                     GKIEIPGEILSKPGRLSPLEFELIKGHSRAGAKILEKIHLPWPLHTIVAQHHERLDGS
                     GYPDGLSGWQILPE"
BASE COUNT          367 a          540 c          457 g          308 t
ORIGIN      
        1 cccgggccgc cgaggagttt cccctcgcca tgaaacggtg ccttcccggg tgctcttctt
       61 ttctgaagga gggacctctc ctccggatcc ggaccatgcg ctcccggtgg ggaagctgca
      121 ccggaaaggg cgtggtcacc ctgaacaccc tgctggtgaa agcatcgccg gcctgcatcg
      181 actacgtctt gatccacgaa ctctgccata ccgtccacca cgaccacggc aagggttttt
      241 actccctgct ggaaagcatc ctccccggat ggaaggaccg gaaaagagaa ctcctggaac
      301 aggcgaaatg ccgccatttc atctgaaaaa ttcttctttt ccgtccaaaa atacccctcc
      361 ggcttattgt gaaagaacat ctttctgcta cactttatag gtatacgccg atgataaccg
      421 tttacttttt cttcttgaag ggaggggcgg tccttgagtc aaacaccgtc cggtcagccc
      481 ggggacaagg cggggaacag cctggaagag cagtccgcgc tgctggagca gctcttcaac
      541 aacgctcccc tccctataca gcgctgcagc atcgaaggac agattctcag ggcaaaccag
      601 gcatcggaag aggtgttcgg ctacacctgc gaggaaatgc gtggccgttt cgtggatgac
      661 ctcttcgctg cgccggatac ctacgatgag gcggtgaaaa acacccggga cgtcctggag
      721 ggaaagaccg tcttcttcga gggcaggcgg cggcggaaga acggcgaggc catctacgtc
      781 aagggaacgg ccttccccat cgtcctgaaa ggggttcaga tcgggaccta cagcatctac
      841 accgaccaga cggccgtaaa ggacgcagag atccgcctca gtcagagcga ggagcggtac
      901 cgttcgctgg tggacgacat ccccgccatg atctgcgcct tcgacgaccg gggcattctt
      961 tccttcgtca accgcgaatt ctgcaccttt ttctccctgc ctgcggaaaa cctcctcgga
     1021 acgtcccttc tccggttcat cccgccggaa catcaccctg caatagtcag ccacctcatt
     1081 tccctttcgg aaaccaagcc ggtcatcacg acggaaatac caaccctccc acccggcagc
     1141 gctcccaggt ggcaccggtg gacagtacgg ctggtctccg aacgggaaaa cgggagacgc
     1201 ctgttccggg ctgcagggca ggacatcacc gattctcggc tcgtccagac caccctggca
     1261 gagaacctcg ccgccatgga gcggaccttc agcgaggcta tagaagtcct tgcatcggcc
     1321 acggacgcga aggaccccta cactgcggaa caccagcgga gggtcgcccg cctttccgcc
     1381 gccatggccg aacagctcgg atatgacagc gccttcatcc gcggagtgga acaggccgcc
     1441 atggtccatg acatcggcaa gatagaaatc cccggtgaaa tcctcagcaa acccggcagg
     1501 ctctcccccc tggaattcga actcatcaag ggacattccc gggctggtgc gaaaattctg
     1561 gagaaaatcc acctgccgtg gcccctccac accatcgtgg cccagcacca cgagcggctg
     1621 gacggaagcg gctaccccga cggtctttca ggatggcaga ttcttcccga gg
//
LOCUS       DAOTHD010000529         1671 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_86173_length_1671_cov_3.226485, whole genome shotgun sequence.
ACCESSION   DAOTHD010000529 DAOTHD010000000
VERSION     DAOTHD010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86173_length_1671_cov_3.226485"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="P5219_08835"
     CDS             <1..400
                     /locus_tag="P5219_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRX26906.1"
                     /translation="MVGKHIPLLPDPSFNPVRPGSPLFFSELPAEEKAALIADNPNYG
                     EIICRCEKITKQEILDAISNPLGARTLVSIKYRARASMGRCQGGFCVPRITRILRDEF
                     GWKPQDFLKRSAGSPLFVGNVRPEKGGAVR"
     gene            397..1659
                     /locus_tag="P5219_08840"
     CDS             397..1659
                     /locus_tag="P5219_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRX26907.1"
                     /translation="MNGAKQSIPAVHRDVVVIGGGPAGLAAAIAAKEAGCGDVLLIER
                     DRILGGILNQCIHDGFGLHTFKEALSGPEYAARFIDRFRELGIAAMEETIVLSLSSDR
                     VLEVSTRGTFQRIEAGAVILAMGCRERTRGAISIPGHRPSGVYTAGAAQNFMNLENIM
                     VGKKVCILGSGDIGLIMARRMTLEGAKVEAVFEILPYSSGLPRNIQQCLNDYGIPLHL
                     NTTVTDIVGKGRLEGIRVAQVGEDRSPIPETERYIPCDTLLLSVGLIPENELTRDAGV
                     EMDPVTGGAVVDDSFMTSVPGIFACGNVLHVHDLVDWVSVEAAEAGKYAACFVREGSC
                     ALPSPIGVKPGSGVRYTLPQSVSGERDVTLSLRVSAPWRDRCVVVKDEGREIARKREM
                     RLHPAEMIRIDLKKETVSGCSSLEVTVE"
BASE COUNT          322 a          505 c          551 g          293 t
ORIGIN      
        1 aatggtgggg aagcacattc ctctcctccc cgatccctcc ttcaatcccg tgcggcccgg
       61 cagccccctg ttcttctccg agctgccggc ggaagagaaa gcggccctca tcgcggacaa
      121 ccccaactac ggcgagatca tctgccggtg cgaaaagata accaagcagg agattcttga
      181 cgccatcagc aatcctctcg gagcccggac gctggtgagc atcaaataca gggccagggc
      241 ctccatgggc cgctgccagg gcggattctg cgttccccgc attacgagga tcctccggga
      301 cgagttcggc tggaagccgc aagacttcct gaagagaagc gccggttcgc ccctgttcgt
      361 gggcaatgtc cggccggaga agggaggagc tgtccgatga acggtgcgaa gcaatcgatt
      421 cccgcggtgc acagggacgt ggtggtcatc ggcggcgggc ctgccggact cgcggcggcc
      481 atagcggcga aggaggcggg ctgcggcgac gttctgctca tcgagcggga ccgcattctc
      541 ggaggcatcc tgaaccagtg catccatgac gggttcggcc tccatacatt caaggaagcc
      601 ctttcgggcc cggagtatgc cgccaggttc atcgaccgct tcagggaact cggcatcgcc
      661 gccatggagg agactatcgt cctctccctg tcatcggaca gggtcctgga ggtgagcacc
      721 cggggaacat tccagcgcat cgaggccggg gccgtgattc tcgccatggg atgcagggag
      781 cgtaccaggg gggccatctc cattccgggg caccgtccct cgggggtgta caccgcaggc
      841 gcggcccaga acttcatgaa tctcgagaac atcatggtgg ggaaaaaggt ctgcatcctc
      901 ggctccggcg acatcggcct catcatggcc cgcaggatga ccctggaagg tgcgaaggtg
      961 gaggcggtct tcgagatact gccctattca agcggccttc cccggaacat ccagcagtgc
     1021 ctcaacgact acggcatccc tctccacctc aacaccacgg tgacggacat cgtgggcaag
     1081 gggcgccttg aaggcatcag ggtcgcccag gtgggcgaag accggtcccc cattcccgag
     1141 acggagcggt atattccctg cgacaccctg ctgctgtccg tgggtcttat ccctgaaaac
     1201 gagctgaccc gggatgccgg tgtggaaatg gatcccgtga ccggcggggc ggtggtggac
     1261 gattccttca tgacgtccgt tccgggtatt ttcgcctgcg gcaacgtcct ccacgtgcat
     1321 gacctcgtgg actgggtctc cgtggaagcg gcggaagcgg gaaaatacgc cgcctgtttc
     1381 gtcagggagg gaagctgcgc cctgccgagc cccatcgggg tgaaaccggg gagcggtgtc
     1441 cggtacaccc ttccccagtc cgtgtcggga gaaagggacg tgaccctgtc cctccgggtt
     1501 tccgcgccgt ggagggaccg gtgcgtggtg gtaaaggacg aaggcaggga gatcgccaga
     1561 aagcgggaaa tgaggcttca cccggcggag atgatccgga tcgacctgaa gaaggaaact
     1621 gtttcgggct gttcctccct ggaggtgacg gttgaatgag cacgagagaa t
//
LOCUS       DAOTHD010000530         1668 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_86401_length_1668_cov_2.556727, whole genome shotgun sequence.
ACCESSION   DAOTHD010000530 DAOTHD010000000
VERSION     DAOTHD010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86401_length_1668_cov_2.556727"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="P5219_08845"
     CDS             complement(<1..263)
                     /locus_tag="P5219_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26908.1"
                     /translation="MRPVHLVYVSAGNGHRMAARAIRESLDHRGVPNVVMDLLDFSSD
                     LFKWSYSDVYAFVSEHSHLACRIMYDLTDKNREESAALRLFEK"
     gene            complement(393..518)
                     /locus_tag="P5219_08850"
     CDS             complement(393..518)
                     /locus_tag="P5219_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26909.1"
                     /translation="MFCEKAESLADILPSLAEKYGENFTSYVIPQGGIVLPMEDA"
     gene            complement(518..>1668)
                     /gene="larA"
                     /locus_tag="P5219_08855"
     CDS             complement(518..>1668)
                     /gene="larA"
                     /locus_tag="P5219_08855"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HRX26910.1"
                     /translation="SCAVKTKLKWGREDIFLSIPDKNVLAVLEPSGGEPVADLAAEVA
                     GLVKRPTAGPSLEEIVSTHKPETAAIIVNDMTRSTPTAEMLPPLLEELERLGVPSSGI
                     AIVVATGTHRPMSDDETRQTVGDAVFSKYRVENHDCDSPDLVDMGTLSTGNRLMINRT
                     VAEADLRLAIGEVLLHYYAGFAGGRKSILPGVAGRETVMTNHRMMTAPGVGIGIVDGN
                     VLSEEMVEAVRDFCPLHFILNCVSDSSKHIVRVVGGHWYDAWREGIVTFRKFNFAPIA
                     KKADVVFLSAGGFPKDINMYQAHKALYMASRAVRPGGPWSSSPSWRKGTATRSSRSGR
                     SGVSASTAPSRPSRPISGSEPTSCTTWPGWRRNAPSCCTPGPAGRTAN"
BASE COUNT          299 a          525 c          518 g          326 t
ORIGIN      
        1 atcttttcga aaagcctgag ggccgcgctc tcctcccggt ttttgtccgt gaggtcgtac
       61 atgatgcggc aggcaagatg ggagtgttcg cttacaaagg cgtagacgtc gctgtaggac
      121 cacttgaaga ggtcgctgga gaagtcgaga aggtccatga ccacgttggg caccccccgg
      181 tggtcaaggg actcccggat ggctctcgcg gccatccgat ggccgtttcc ggcgctgaca
      241 taaacgagat gaacgggtcg cacggagttt cccccctggg ctttccggaa gcgtccgggc
      301 gggcggactt cgcgagtcgc ccgcccggga aaggcatcct gataatgagc tgcccttcat
      361 tgtaccccat gaagggcaaa gggggaactc tttcaggcat cctccatggg gaggacgatg
      421 cccccctggg gaatcacgta ggacgtgaaa ttctcgccgt acttctccgc cagtgagggc
      481 aggatgtccg ccagggactc cgccttctcg cagaacatca gttcgctgtc ctccctgccg
      541 gacctggagt acagcaggat ggtgcattcc ttcgccagcc gggccaggta gtacagcttg
      601 tgggctccga accggaaatc ggcctcgaag gcctcgatgg cgccgtcgat gccgagacgc
      661 cgcttcgccc actcctcgaa gaccttgtgg ccgtaccctt ccgccagctc ggcgaagaag
      721 accagggtcc cccggggcgg acggccctcg aggccatgta gagggccttg tgggcctggt
      781 acatgttgat gtccttgggg aatccccctg ccgacaggaa caccacgtca gcctttttcg
      841 caatgggggc gaaattgaac ttcctgaagg tgacgatgcc ctcccgccag gcgtcgtacc
      901 agtggccccc caccacccgg acgatgtgct tcgagctgtc ggacacgcag ttgaggataa
      961 agtggagcgg acagaaatcc cgcaccgcct ccaccatttc ctcgctgagg acgttgccgt
     1021 caacaatgcc gatgcccact ccgggggcgg tcatcatgcg gtggttcgtc atcaccgttt
     1081 cccggccggc cactccgggg aggatactct tcctcccccc cgcaaacccg gcatagtagt
     1141 gcaggagcac ttcgccgatg gcgagcctga gatcggcctc tgccacggtc ctgttgatca
     1201 tcagccggtt gccggtggag agggttccca tgtccacgag atcgggggaa tcgcagtcgt
     1261 ggttctccac ccggtatttg gaaaagacgg catccccgac ggtctgcctc gtttcgtcgt
     1321 cgctcatggg acggtgggtg cctgtggcca ccactatggc gatccccgag gagggaacgc
     1381 ccagccgctc cagctcctcc agcaggggag gaagcatctc ggccgtgggg gttgaccggg
     1441 tcatgtcgtt gacgatgata gccgccgtct ccggtttatg ggtggagacg atttcctcca
     1501 gggacggccc cgccgtgggg cgcttcacaa gcccggcaac ctcggcggcc aggtcggcca
     1561 cgggctcccc tcccgagggt tcgagcaccg ccagaacgtt tttgtcgggg atggaaagga
     1621 agatatcctc ccttccccat ttcagtttcg ttttcacagc acatgacc
//
LOCUS       DAOTHD010000531         1667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_86463_length_1667_cov_3.590571, whole genome shotgun sequence.
ACCESSION   DAOTHD010000531 DAOTHD010000000
VERSION     DAOTHD010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86463_length_1667_cov_3.590571"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /gene="kynU"
                     /locus_tag="P5219_08860"
     CDS             complement(<1..1272)
                     /gene="kynU"
                     /locus_tag="P5219_08860"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HRX26911.1"
                     /translation="MDERFSTYRARAEEMDRWDPLKSFRDQFHIPPETAYMDGNSLGL
                     PPRAAGDALEKTFSEWRDLAIGGWLEGTPPWFHLAEEAGRKMAPLMGASPDEVVMSGS
                     TTVNLHALVSTFYKPSGTRTKIVADELNFPSDIYALESQIRLKGFDPKEHLVLVRSRD
                     GRTLDEDDIIAAFDDRAALALLPGVLYRSGQLLDMERLVKEAHNRGILIGFDCAHSAG
                     AVPHRLSDWDADFAFWCSYKYLNGGPGSPAFLYVNRRHFDLEPGLAGWFGCDKSRQFD
                     MDLEFSHARCAGGWQIGTPPVLSASPLLPSLDIFARAGMERLRHKSLALTGFLAEIIE
                     GSLSSAPYGYSSGTPREDSRRGGHLAVEHPEAWRICQALKQRKIIPDFRPPQVIRLAP
                     VPLYNSFGDVLRVAEALKEIVDGKEYEAFSPV"
BASE COUNT          306 a          490 c          522 g          349 t
ORIGIN      
        1 gacgggggaa aaagcctcgt actccttgcc gtccacgatc tccttcagag cttccgcaac
       61 cctgaggacg tccccgaagg agttgtagag aggaacgggt gcaaggcgga tgacctgtgg
      121 cggcctgaaa tcagggatga tcttccgctg cttcagagcc tggcatatcc tccatgcctc
      181 cgggtgctcc acggccaggt ggccgccccg acggctgtcc tccctggggg tccccgagga
      241 gtagccgtag ggagcggagg agagcgatcc ttcaatgatt tcggcgagaa agcccgtaag
      301 ggcgagggat ttgtgccgca gccgctccat gccggccctg gcgaatatgt cgagggacgg
      361 aagcaggggg gaggcggaga gcaccggcgg ggtgccgatc tgccatcctc cggcgcaccg
      421 ggcatggctg aactccagat ccatgtcgaa ctggcggctc ttgtcgcatc cgaaccagcc
      481 cgcaagtccc ggctccaggt cgaaatgtct ccggttgacg tacaggaacg cggggcttcc
      541 ggggccgccg ttcaggtact tgtagctgca ccagaaggcg aaatcggcgt cccagtctga
      601 aagcctgtgg ggaacggccc ctgcggagtg ggcgcagtcg aagccgatga gaatgccccg
      661 gttgtgggct tctttcacga ggcgttccat gtccaggagc tgaccgctcc ggtagagcac
      721 ccccggaagg agggccaggg ccgcccggtc gtcgaaggcg gcgatgatgt catcctcgtc
      781 gagggtgcgt ccgtcccggg agcggaccag cacgaggtgc tccttcgggt cgaatccttt
      841 cagacggatt tggctttcaa gagcgtagat gtccgaggga aagttcagct cgtcggccac
      901 gatcttcgtc cgtgttcccg agggcttgta gaaggtgctt accagggcgt ggaggttcac
      961 cgtggtggag ccggacatga ccacttcatc gggggatgct cccatgaggg gcgccatctt
     1021 ccgtcccgcc tcttccgcga gatgaaacca tggaggggtt ccctcgagcc agccccctat
     1081 ggccaggtcc cgccattcac tgaaagtttt ctcaagggcg tctccggcag cccggggcgg
     1141 cagcccaagg gagttgccgt ccatgtaggc cgtttccgga ggaatgtgga actggtcccg
     1201 gaaggatttc agaggatccc acctgtccat ttcctccgcc cgggcccggt acgtcgagaa
     1261 tcgttcatcc ataatccgcc accctttcgt gtgtttcatt ttttcgagaa tagcacaccg
     1321 gggcggaaat atggagggta ttctcccata aatgtattct cccataaatg ttgacaggcc
     1381 cttaatgggg aaatatgatc gacagggctg taacgcccga ctgtttccac tccttcgaga
     1441 atacggatca cgatgccttt ttcattccac tgcagaactg acgatgaagg gagctgcaaa
     1501 cattcatgtc ttcttccggc cctgctgtac gttttctccg gtatttcctt ccattattcc
     1561 tcagtctttg tgtccttgca ttttcaatcg ctccggcccc ggctgcggtc tggcatgtga
     1621 ggccgtccgg caccggaggc gggtcggact gggacaacgc gtctgcg
//
LOCUS       DAOTHD010000532         1666 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_86553_length_1666_cov_2.816263, whole genome shotgun sequence.
ACCESSION   DAOTHD010000532 DAOTHD010000000
VERSION     DAOTHD010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86553_length_1666_cov_2.816263"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="P5219_08865"
     CDS             <1..853
                     /locus_tag="P5219_08865"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRX26912.1"
                     /translation="LAPGDPAEVMLGPSATQEEITNLREQLGLTKPLYVQYGIFLTNA
                     LKGDLGRSIKSNAPVVDEIMERFPATLTLAVLGMIFAIAIGLPLGILAALKQNTWVDA
                     LCSFFALVGFSIPNFWVGLMLMLLLSIYIPILPSSGFDSWKNFIMPTIVLGIQTMAVI
                     ARMTRSSMLEVIRQDYVRTARAKGISDRLVLTRHILGNVLIPVVTIAGIYFGHQLGGV
                     VITETIFSIPGLGRLIVDAIRAQDYPVVQGGVLFFALCVGIVNLLVDLLYAVLDPRIK
                     AQYRAAK"
     gene            873..1666
                     /locus_tag="P5219_08870"
                     /pseudo
     CDS             873..1666
                     /locus_tag="P5219_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164791.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
BASE COUNT          293 a          553 c          498 g          322 t
ORIGIN      
        1 cctggcgccg ggagaccccg ccgaggttat gctcggcccc agcgccaccc aggaggaaat
       61 taccaacctg agggagcagc tgggactcac caagccttta tatgtccaat acggcatctt
      121 tctcaccaac gccctcaagg gtgatctcgg ccggtccatc aagagcaacg ccccggtggt
      181 ggacgagata atggaacggt ttcccgccac cctgaccctc gcggtgctcg gcatgatctt
      241 cgccatcgcc atcggtctgc cgctgggaat tctggcggca ttgaaacaga acacctgggt
      301 ggacgcctta tgcagcttct ttgcccttgt gggcttctcc ataccgaact tctgggtcgg
      361 cctgatgctc atgctgctgc tctcgatcta cattcccatc cttccgtcgt cgggtttcga
      421 ctcctggaaa aatttcatca tgcccaccat cgtcctcgga atccagacca tggcggtcat
      481 cgcacggatg acccggtcga gcatgcttga ggtcatccgg caggactacg tgcgcaccgc
      541 cagggccaag ggcatatccg accggctggt gctcaccagg catattctcg gcaacgtgct
      601 gatccccgtg gtgaccatcg cggggattta tttcggccac cagctcggcg gcgtggtcat
      661 caccgagacc atcttttcca ttccgggcct cgggcggctt atcgtggacg ccatccgcgc
      721 ccaggattac cccgttgtgc aggggggcgt gctgttcttc gccctctgcg tggggatcgt
      781 gaacctcctg gtcgacctgc tctacgccgt actggacccg cggatcaagg cccagtaccg
      841 ggcggcgaaa taagggagag ggggataacg tcatgaacaa tgaaaacgga accctgttcg
      901 ctccctcatc cggccggacg aaggaaagag aaaagcgccg gagcccctgg tgggaagtct
      961 ggcggcgcct gcggcgcaac aaggccgccg tcgcgggaat gtcggtggtc atcctgcttg
     1021 tggtggtggc ggtccttgcc cccgtcctgg caccctacga cccgaaccag accaacataa
     1081 aaatgcgcct ccagggaccg tcgtcggaac accccctggg aacggacaac ttcggccggg
     1141 acatgctgag ccggatcatc tacggcagca gaatctctct ttatgtcggc ttcgttgccg
     1201 tcggcatcgg cgccgtcttc ggcggcatcc tcggagctat cggcggctac tacggcggcc
     1261 ggctcgacaa cttcatcatg cggtgcatgg acgtgctgct ggccataccc cagatcatcc
     1321 tcgccatcgc catcgtcggt gccctgggaa ccagcctgct gaacctcatg atcgccgtgg
     1381 ggatcagcca gcttccccgg tatgcccggg tggttcgggc atcggccctc accgtccggg
     1441 ggcaggagta cgtggaggcg gcccgggcgg tgggagcgag cgacgccagg atcatcctgg
     1501 agaacatcct ccccaactgc atggctccca tcatcgtcca gagcaccctg ggagtggccc
     1561 aggccatcct ttcggcggcg gccctctcct tcctcggcct cggcatacag cccccacggc
     1621 ggagtggggc tccatgctca gctcggggcg gcagttcctc cggaac
//
LOCUS       DAOTHD010000533         1661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_86915_length_1661_cov_2.608344, whole genome shotgun sequence.
ACCESSION   DAOTHD010000533 DAOTHD010000000
VERSION     DAOTHD010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86915_length_1661_cov_2.608344"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1180
                     /locus_tag="P5219_08875"
     CDS             164..1180
                     /locus_tag="P5219_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRX26913.1"
                     /translation="MEEKTPSCLEIRKLGPGDAFKGIGVVQKLNRRSDKNGRPFWEVT
                     LSDATGSFDGKVWGDAAWWDLSKGEKSAIDPTQEAFSRNLRGKSLGVQGKMVEFRGQP
                     QVNVTALYVLDQVKYPPSLFVQASPISLEELEKSFRELVDSEAGRFAPFLRHVFSGEF
                     WERFKQSPAAVTHHHAYAHGLLEHTVSVAGLALAMGHAASGNPHADVDLETVVAGALL
                     HDLGKVESYTLSPVPEMTLPGAVLDHVGPGFARFVRLAEAWGLDERSTLALGHIVLSH
                     HGQKEFGAAVLPATPEALIVSSADELDFRLFCWGDATGNLLEGDEVSDFHYSAQRRFW
                     KWQE"
     gene            1255..1488
                     /locus_tag="P5219_08880"
     CDS             1255..1488
                     /locus_tag="P5219_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255349570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1285..1287,aa:Sec)
                     /transl_table=11
                     /product="Rdx family protein"
                     /protein_id="HRX26914.1"
                     /translation="MKIRLEYCTAUGYLERALGVAGTILGKYKNQVESFELVPSGGGV
                     FEFSVNGELVYSKKATGLHTDPDFLLGEMERFL"
BASE COUNT          344 a          463 c          528 g          326 t
ORIGIN      
        1 ttccctttat cctccagagt tcctggtgca ggccggacga ggtgctcgcg ttcggcgaac
       61 gcatggtggc gcgcggagtt caagggaaga tcccggctca caccctcatg ggactgatgc
      121 tggcgcacgg gctgcggctg gccaaattcg gcgcttgatc gagatggaag aaaagactcc
      181 cagctgcctg gagattcgca agcttggccc cggggacgcg ttcaagggaa tcggcgtcgt
      241 ccagaaactg aacaggagaa gcgacaagaa cgggcgcccg ttctgggaag tcactctctc
      301 cgacgccacg gggagtttcg acggcaaggt ctggggagac gccgcgtggt gggatttgtc
      361 aaagggtgaa aaatccgcca ttgaccccac ccaggaagcc ttctccagga acttgcgcgg
      421 caagtctctc ggggtgcagg gcaagatggt ggagtttagg ggccagcccc aggtcaacgt
      481 gacggccctc tatgtgctcg accaggtaaa gtacccgccg tccctgtttg tccaggcctc
      541 tccgatctcc ctggaggaac tggaaaagtc cttcagggaa ctggtcgact ccgaggcggg
      601 gcgtttcgct cccttcctgc ggcatgtctt ctcaggggaa ttctgggaga ggttcaagca
      661 atccccggca gcggtcacgc accaccacgc ctatgcccac ggattgctgg aacacaccgt
      721 ttccgtggcc gggctggctt tggcgatggg ccacgcggca tcgggaaacc cgcatgcgga
      781 cgtggacctg gaaacggtgg tggcgggggc cctgctccac gacctgggaa aagtcgagtc
      841 ctacaccctt tccccggtcc ctgaaatgac cctgccggga gcggtgcttg accacgtcgg
      901 cccagggttt gcccggttcg tccgcctggc ggaggcctgg ggccttgatg aacgctcgac
      961 cctggccctt ggccatatcg tgctgagcca ccatgggcag aaggaattcg gtgcggccgt
     1021 tttgcccgcg acacccgagg cactgatcgt ctcttccgcc gacgagctcg atttccggct
     1081 tttttgctgg ggggacgcga caggcaacct gctggaaggg gacgaggtca gcgattttca
     1141 ctactctgcc cagagacgtt tttggaaatg gcaggagtag cacaggatat tcttatatga
     1201 tgtgataaaa tgacagagtg aaagaacccg gggttcgagg aggtgcaagc catggtgaaa
     1261 ataaggctcg agtactgcac agcctgaggg tacctcgaaa gggctctcgg cgttgccggg
     1321 acaatacttg gcaagtacaa gaaccaggtc gaatcctttg aacttgtgcc ttccggcgga
     1381 ggagtcttcg agttttccgt caacggggaa ttggtctaca gcaagaaggc gaccgggttg
     1441 cacacagacc ccgatttcct cctgggggag atggagcggt tcctctgaaa agacaggctc
     1501 cggccgataa cgcggcgaaa ggatgtttcg ccgcaatttt tttgttcagg aggcatccag
     1561 accgtatgac atacaccttc aagacatctg aaaacgactc gggccgcagg ctcgaccgaa
     1621 tattaaggac acgctggcct caggtgccgc tgggaaggat a
//
LOCUS       DAOTHD010000534         1658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_87094_length_1658_cov_3.367436, whole genome shotgun sequence.
ACCESSION   DAOTHD010000534 DAOTHD010000000
VERSION     DAOTHD010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87094_length_1658_cov_3.367436"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1367)
                     /locus_tag="P5219_08885"
     CDS             complement(<1..1367)
                     /locus_tag="P5219_08885"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HRX26915.1"
                     /translation="MAEAVQKKSRVPHVFALMFIITILMAVLTWLIPAGEYERVKEGA
                     RTVVVANSFKVVDSNPQGFWGVFDAVVKGWIQSASMIFMVFFVGGAIKILEETGTIRV
                     GMNRIVHKLKGKELWAVAIIMLLMSIGGATGVFANPVVALIPLGILLAKGLGYDSVVG
                     FAMIYLGSYAGFNVGWGNVFTVGIAHSIAELPMFSGFGVRVFFHVVNLLLTFGFVYLY
                     IRKIQADPTKSLVYSAEEAAQQTNHFEEHESMDLRHMLCTAIVVISFGAIIYGSLQLK
                     WGINHYSAIFLMMAVFCGLIGGLGLNGTAQAFVKGCGSMAYAALVIGMARAISVVMSD
                     GKIIDTVVYYLSLPIAKYGPVIGANLMFFANVVINFFIPSGSGQAVTVMPIMVPLADL
                     TGITRQVAVQAFQFGDGFTNCFIPTASVVMGCLGLSGIAYEKYVKWIFPLILTQVVLA
                     MVALTV"
     gene            complement(1389..>1658)
                     /locus_tag="P5219_08890"
     CDS             complement(1389..>1658)
                     /locus_tag="P5219_08890"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRX26916.1"
                     /translation="TERIARAAEDVLGEDRVIRLAAPMMGSEDFAYFIQGVPAGVIFR
                     LGVGGEIPVSLHNPGFNFPDEALPLGVAVFVRYIFDVLAPEGAKS"
BASE COUNT          352 a          461 c          544 g          301 t
ORIGIN      
        1 actgtgaggg caaccatggc cagtacgacc tgggtgagga tgagggggaa gatccacttc
       61 acgtacttct cgtaggcgat gcccgaaaga ccgaggcatc ccatgacaac gctcgccgtg
      121 gggatgaagc agttggtgaa gccgtcgccg aactggaaag cctggacggc cacctgccgg
      181 gtgattcccg tgagatccgc caggggcacc atgatgggca tgacggtaac cgcctgtccc
      241 gagcctgagg ggatgaagaa gttgatgacc acgttggcga agaacatgag gttcgcgccg
      301 atgacggggc cgtacttggc gatgggcagg gagaggtagt agaccaccgt gtcgatgatt
      361 ttgccgtcgc tcatgaccac ggagatggcc cgggccatgc cgatgacgag agctgcgtag
      421 gccatggaac cgcatccctt gacgaaggcc tgggcggtgc cgttcaggcc gaggccgccg
      481 atgagaccgc agaagacggc catcatcagg aaaatggcgg agtagtggtt gatcccccac
      541 ttgagctgca gggaaccgta gatgatggcg ccgaaggaga tgaccacgat ggccgtgcag
      601 agcatgtgcc ggaggtccat ggactcgtgc tcttcaaaat ggttggtctg ctgggccgct
      661 tcttcggcgg agtacaccag gctcttggtg gggtcggcct ggatcttcct gatgtagaga
      721 taaacgaatc cgaaggtgag aaggagattc acgacgtgga agaagacccg gacgccgaag
      781 ccggagaaca tgggaagttc ggcgatgctg tgggcgatgc ccacggtgaa gacgttgccc
      841 cagccgacgt tgaagccggc gtaggagccg aggtagatca tggcgaagcc caccacgctg
      901 tcatagccga ggcccttggc aaggaggatg cccaggggga tgagggcaac cacggggttg
      961 gcgaagacgc cggtggcgcc gccgatggac atgagaagca tgatgatggc cacggcccag
     1021 agctctttgc ccttcagctt gtggacgatc cggttcatgc ccacccggat ggtgcctgtc
     1081 tcctcaagga tcttgatggc cccgccgacg aagaaaacca tgaagatcat ggaggcggac
     1141 tggatccatc ccttgacgac ggcgtcgaaa acgccccaga agccctgggg gttggaatcg
     1201 accactttga aggagtttgc tacgacgacg gtcctggctc cttctttcac cctttcgtac
     1261 tctcccgcag gaatcagcca ggtcagtacc gccataagga tggtgatgat gaacatcagc
     1321 gcgaatacgt gtggaaccct gctctttttc tgcactgctt ctgccattgc tctctctcct
     1381 tttcgtttct aggatttcgc cccttcgggg gcgagcacgt cgaaaatata ccggacgaag
     1441 accgccacac ccaggggcag tgcctcgtcg ggaaaattga agcccgggtt gtggagggac
     1501 accgggatct ctccgcccac tccgagacgg aagatgaccc ctgcgggcac gccctggatg
     1561 aagtaggcga agtcctccga gcccatcatg ggggcggcga gacggatgac cctgtcctcg
     1621 ccgaggacgt cctccgccgc ccgtgcgatc cgttccgt
//
LOCUS       DAOTHD010000535         1658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_87111_length_1658_cov_2.758578, whole genome shotgun sequence.
ACCESSION   DAOTHD010000535 DAOTHD010000000
VERSION     DAOTHD010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87111_length_1658_cov_2.758578"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="P5219_08895"
     CDS             complement(<1..497)
                     /locus_tag="P5219_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRX26917.1"
                     /translation="MTLSNKLFYFFVKWFFYISLKVYNRISIRWLSPLPRRNYIVIAN
                     HCSNLDPVVMGAIFPGRLRFLAKAELFEPFIFGSMVRALGATPVQKQDSQSAGAALRA
                     FLKLLDDGENVLLFPEGGRSLNGKLQPLEGGAALIALKSGAPVVPAFVAGTFEAMPPG
                     SKFVKP"
     gene            complement(481..1173)
                     /gene="cmk"
                     /locus_tag="P5219_08900"
     CDS             complement(481..1173)
                     /gene="cmk"
                     /locus_tag="P5219_08900"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRX26918.1"
                     /translation="MTDQPLIVAIDGPAGAGKSTIAKLVAGFLGIPYLDTGALYRALA
                     LFLDARGIPPVESDALFSALQEASLEITEKEILLEGKNVGAEIRTPRIDAVVSAYASL
                     PMVRNRLLDLQREQGKRNGLVADGRDMATVVFPDARVKIFLTASDEERARRRFLELRA
                     RKESTGYEDVLEQIRERDRTDSSRSTAPLRKADDAVELSTDGLSIGEVTEKVLNLIRE
                     ILDKENHYDAVQ"
     gene            complement(1170..>1658)
                     /locus_tag="P5219_08905"
     CDS             complement(1170..>1658)
                     /locus_tag="P5219_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRX26919.1"
                     /translation="KTRKALPEEAYVGGLLHDIGKVVLNDYVRFGYGIIVKLVEEQQI
                     PFLDAEVQVLGFDHAAVGTLLVEKWGLPDPFRFAVAHHHTPNALQEPKYQSLVDVVHI
                     ANSLCLMLGVGLGADGLQSYLYPESLERLGITDYETLMSELVDFVAQAEAELSEMEEA
                     FQ"
BASE COUNT          369 a          460 c          458 g          371 t
ORIGIN      
        1 ggtttgacga attttgagcc gggaggcatg gcctcgaacg taccggcgac aaaggctgga
       61 acgaccggtg cgcctgactt cagggcaata agggcggctc ctccttcaag aggctggagt
      121 ttgccgttca gggatcttcc tccttcggga aaaagaagca cattttcacc gtcatcgagc
      181 agcttcagaa aagccctcag ggcggctccg gcgctctggc tgtcctgctt ctgcaccgga
      241 gtggcaccca gggcccggac catgctcccg aaaataaagg gctcaaacag ttccgctttg
      301 gccagaaagc gcagtcttcc ggggaatata gctcccatga ccacggggtc aagattgctg
      361 cagtgattgg ctatgacgat gtagttccgg cggggaagcg gagagagcca tcggattgaa
      421 atcctgttgt acaccttgag agagatgtaa aagaaccact ttacaaaaaa ataaaacaat
      481 ttattggaca gcgtcataat gattctcctt gtccaggatt tcccttatca gattgagcac
      541 cttctctgtc acttccccga tggagagtcc gtcggtactc agttccacgg cgtcatcggc
      601 ctttctcaga ggagccgtgg agcggctgct gtctgtcctg tccctctccc gtatctgttc
      661 cagcacatcc tcataaccgg tgctttcctt tcgcgccctg agctcgagaa aacggcgacg
      721 tgccctttcc tcgtctgacg cggtaagaaa aattttgacc ctggcgtcag gaaatacaac
      781 ggtcgccatg tcacggccgt ccgctacgag accatttctc tttccctgtt cccgctggag
      841 gtcaaggagc cggttcctga ccatgggcag cgatgcgtag gcggaaacaa cggcatcgat
      901 acggggagta cggatctcgg ccccgacgtt ttttccctca agcagtattt ctttttcggt
      961 tatctccagg gatgcctcct gaagggcgga aaacagagca tccgattcca ccggcggaat
     1021 gcccctggcg tcaagaaaaa gggcaagagc cctgtacaag gctccggtgt caaggtaggg
     1081 aatccccagg aatccggcga ccagtttagc gatggtgctt ttcccggctc ccgccgggcc
     1141 gtcgatggcc acaatgagcg gctgatccgt cattggaaag cttcctccat ttccgacagc
     1201 tcggcctctg cctgggcgac gaaatccaca agttcggaca tgagagtttc gtaatccgtg
     1261 atgccgagcc tttcaaggga ttcgggatag agatagctct ggagaccgtc ggcaccgagt
     1321 ccaacgccaa gcatgaggca gagggaattg gctatatgga ccacgtcgac cagggactga
     1381 tatttcggtt cctgcagagc gttgggggtg tggtgatgag ccaccgcgaa tctgaagggg
     1441 tcgggaagcc cccatttttc aaccagcagg gttcccacgg ctgcgtggtc gaaaccaaga
     1501 acctgcactt ccgcgtcaag gaaaggaatc tgctgttcct ccaccagttt gacgatgatg
     1561 ccgtatccga aacggacata atcgttcagc acaacttttc ctatatcgtg aagaagcccg
     1621 ccgacatagg cttcttccgg aagggctttc cttgtctt
//
LOCUS       DAOTHD010000536         1658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_87117_length_1658_cov_2.632564, whole genome shotgun sequence.
ACCESSION   DAOTHD010000536 DAOTHD010000000
VERSION     DAOTHD010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87117_length_1658_cov_2.632564"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="P5219_08910"
     CDS             complement(<1..554)
                     /locus_tag="P5219_08910"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRX26920.1"
                     /translation="MNRTIKKLAWLFCAALIVLTATVPTAAEPEKRLRTASFDGVGVS
                     YLDEGPGDALPVVFIHGWACTGDFWRFQTSALRDRYRVIALDLPGFGKSGKPHDRAYT
                     LRFFARAVKAVIDHAGVKRPVLAGHSMGYGVAVQFLADYPGEARGLCNADGAFFWLPD
                     DPGEVPPWEAEMKGLSDGLSGPDR"
     gene            complement(709..>1658)
                     /locus_tag="P5219_08915"
     CDS             complement(709..>1658)
                     /locus_tag="P5219_08915"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRX26921.1"
                     /translation="KGQLMAHILAVRAFRETATDIPCSVKFVFEGEEESGSTSLAPFV
                     EQNRERLACDIALAVDGPMLSGDVLNVCLGCRGVLNFEIEMETARFDCHSGRGGDIIP
                     NAGWELLRLLRTMKDEQGSILVEGFMDGVRTPTEKDMEIIAGLPFDRENLKNLYGVPS
                     FGLDKNEYYTKLNLLPTLTINGITCGYQGKGSKTINPAKASVKMDARLVVDQNPLDVA
                     EKIKGHVARHAPGARVTVHGFMYPSKTDGDLPVCRTVVSALEKVYTDKVALSLGLGGS
                     LPSYVWTDILGVPALGIPYANQDSGGHAPNENFRLDLPQ"
BASE COUNT          278 a          539 c          522 g          319 t
ORIGIN      
        1 tcccggtccg gacctgaaag cccgtcggaa aggcccttca tctccgcctc ccatggcggg
       61 acctccccgg ggtcgtcggg aagccagaag aaggccccgt cggcattgca gaggccccgg
      121 gcctcgccgg gatagtcggc gaggaactgg acggccaccc cgtagcccat gctgtgcccg
      181 gcaagcacag gccgcttcac gccggcatgg tcgataacgg cctttaccgc ccgggcgaag
      241 aaccggaggg tgtaggccct gtcatggggc tttccgctct tcccgaagcc gggaagatcc
      301 agggcgatca cccggtaccg gtcccgcagg gcggacgtct ggaaacgcca gaaatccccg
      361 gtgcaggccc agccgtggat gaagaccacc ggcagggcgt ccccggggcc ttcgtccagg
      421 tagctcaccc cgacgccgtc gaaggaggcc gtccgcaggc gcttctccgg ttcagccgcc
      481 gtagggacgg ttgccgtcag cacgatcagg gcggcacaga acagccacgc cagttttttt
      541 attgtccggt tcattgatac cagcctccgc gattcaaagt tttctttccg ggccttggat
      601 ggccctattc tacatccgcc ggaggaagaa ggggaaactc ccctcccctc tttccgccgg
      661 aaggaactat ttcatgtcgt gcagtatctg ggctgaggca tggaccgcct actgcgggag
      721 atccaggcgg aagttctcgt tgggtgcgtg gcccccgctg tcctggttgg cgtagggaat
      781 ccccagggcc ggaacgccga ggatgtcggt ccatacgtag ctcggcaggc ttcctccaag
      841 gcccagggag agggcgacct tgtcggtata cactttttcc agggctgaga ccaccgttct
      901 gcacaccgga agatctccgt cggtcttcga cgggtacatg aagccatgga ccgtcacccg
      961 cgctccgggg gcatggcggg cgacatgccc cttgattttc tccgcgacgt cgagggggtt
     1021 ctggtccacc acgagccggg cgtccatctt caccgaggct ttcgccggat tgatggtttt
     1081 ggaacccttc ccctggtagc cgcaggtaat cccgttgatg gtaagcgtgg gcaggaggtt
     1141 gagcttcgtg tagtactcat ttttgtcgag gccgaaggag ggcacgccgt agaggttttt
     1201 gaggttctcc ctgtcgaagg gcagcccggc gatgatctcc atgtcctttt ccgtgggcgt
     1261 ccggaccccg tccatgaacc cttcgaccag gatgctgccc tgctcgtcct tcatggtccg
     1321 gagcaggcgc agaagctccc atccggcgtt gggtatgatg tccccgcccc ggccggaatg
     1381 gcagtcgaac cgggcggttt ccatctcgat ctcgaagttc agcacacccc tgcaccccag
     1441 gcagacgttg agcacgtccc cggaaagcat gggcccgtcc acggcgagag cgatgtcgca
     1501 ggccagcctc tccctgttct gttccacgaa gggggcgagg ctggtgctcc ccgactcctc
     1561 ctctccctcg aagacgaact tcacgctgca gggaatgtcc gtcgccgtct cccggaaggc
     1621 ccggacggcc aggatgtggg ccatgagctg ccccttgt
//
LOCUS       DAOTHD010000537         1653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_87469_length_1653_cov_3.265957, whole genome shotgun sequence.
ACCESSION   DAOTHD010000537 DAOTHD010000000
VERSION     DAOTHD010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87469_length_1653_cov_3.265957"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="P5219_08920"
     CDS             <1..726
                     /locus_tag="P5219_08920"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRX26922.1"
                     /translation="RAFSILIHLAQEKRPLGITEIASAVSLPKATVYRILDSLEAERA
                     VLNRGGEYELGPVTLLLADAYRSQVGFAEAARPHLLSLRNETKETVHLFVYERGEFYY
                     LDKIESPYQVRMHSRIGGRASLFRLSAGKALLAGLSPDDLKELHEPVPGEVAAEFPGI
                     LSRGYAVDDEQNEQGLRCVGAAIHDGAGRPRGAVSVSAPVYRFPAELLEKYGSLVARA
                     AEAIGREIACFEECPCSGGRSAG"
     gene            763..>1653
                     /locus_tag="P5219_08925"
     CDS             763..>1653
                     /locus_tag="P5219_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRX26923.1"
                     /translation="MIKTNKLVMIPGPTPVVRSIQDQMARETVAFGDAAFVKDFSEVI
                     ADLKAMWRCDGEVFVVAGSGTMGMEMAIANTTKRGDNVLICSNGYFGDRFIDMCTRKG
                     LNTDVLSAEWGKSVTPEMVEKKLAEKKYQVVTVTHVETSTGVEAPIAAIGEVMKKHPE
                     IIYIVDGVAASGGADQYMDTMGIDVVLSCTQKAFGVAPGLTMVWASAKAMEKRKSLGA
                     IPESYIDFDKWLPVMHDPSKYWGTPAINLVWALKESVRIMKEEGLEERYARHRRQAAM
                     FDAALEAIGFRVAAEKAFRAP"
BASE COUNT          328 a          523 c          521 g          281 t
ORIGIN      
        1 cgggcttttt ccatactgat tcacctcgcg caggaaaaac ggcctctcgg aatcaccgag
       61 atcgcgtccg ccgtctccct gcccaaggcc accgtgtacc gcattctcga tagcctcgag
      121 gccgagcggg ccgtcctgaa ccggggcggc gagtacgagc tcggtcccgt gaccctgctt
      181 ctggcggatg cctaccgcag ccaggtcggc ttcgccgagg ctgcccggcc tcacctcctc
      241 tccctcagga acgagaccaa ggaaacggtc cacctcttcg tctacgaacg gggagagttc
      301 tactatctcg acaagatcga aagcccctac caggtgcgta tgcactcccg catcggaggc
      361 agggcttccc tgttccggct ctccgccgga aaggcccttc tcgccggcct gtcccccgac
      421 gacctgaaag aacttcatga accggttccc ggcgaagtcg ccgccgagtt ccccggcatc
      481 ctttccaggg ggtatgccgt ggacgacgag cagaacgagc aggggctccg gtgcgtggga
      541 gcggccatcc atgacggtgc gggacgcccg aggggtgccg tgagcgtctc cgccccggtg
      601 taccgcttcc cggccgaact cctggagaaa tacggttccc tcgtcgcccg ggcggcggag
      661 gccatcggca gggagattgc ctgtttcgaa gagtgcccgt gttccggcgg gcggtccgcc
      721 ggttgaaaaa agaaaaagag aaaacagaag gaggaagaca ctatgatcaa gaccaacaag
      781 ctcgtcatga tacccggacc cacccctgtg gtccgctcca tccaggacca gatggccagg
      841 gagaccgtgg ccttcggcga tgccgcattc gtgaaggatt tttccgaggt catcgccgac
      901 ctgaaggcca tgtggcggtg cgacggcgaa gtgttcgtcg ttgcgggctc gggcaccatg
      961 ggcatggaaa tggccatcgc caacaccacg aagcggggcg ataacgtcct tatctgctcc
     1021 aacggctact tcggcgacag gttcatcgac atgtgcaccc ggaagggact caacaccgat
     1081 gttctctccg ccgaatgggg aaaatcggtg accccggaga tggtggagaa gaagctggcg
     1141 gagaagaagt accaggtggt gacggtgacc cacgtggaga cctccaccgg cgtggaggcg
     1201 cccatcgctg ccatcggcga agtgatgaag aagcaccctg agatcatcta catcgtggac
     1261 ggagtggctg cctccggcgg cgcagatcag tacatggaca ccatgggcat cgacgtggtg
     1321 ctttcctgca cccagaaggc cttcggcgtg gcgcccgggc tgaccatggt gtgggccagc
     1381 gcgaaggcca tggagaagag gaagagcctc ggtgccattc ccgagtccta catcgacttc
     1441 gacaagtggc tccccgtcat gcacgatccc tcgaagtact ggggaacgcc tgccatcaac
     1501 cttgtctggg cgctgaagga gtccgtacgg atcatgaagg aagagggcct cgaggagcgc
     1561 tatgcccgcc accgccgcca ggcggccatg ttcgatgccg ccctggaggc catcggcttc
     1621 agggtcgccg ctgaaaaagc cttccgtgcg ccc
//
LOCUS       DAOTHD010000538         1653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_87486_length_1653_cov_2.579474, whole genome shotgun sequence.
ACCESSION   DAOTHD010000538 DAOTHD010000000
VERSION     DAOTHD010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87486_length_1653_cov_2.579474"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            542..>1653
                     /locus_tag="P5219_08930"
     CDS             542..>1653
                     /locus_tag="P5219_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26924.1"
                     /translation="MKKILLALLVLFFLRGNSRAELRIGLPDLERELTSSPEVLAAAA
                     SLSESRAVEERQKMTGGPRLFGELSLGGLNEPASRSETSALEGYTKVWARGGISIPLF
                     GTWQKERIRELENRIISLGEELRLFAVRKANLTALRKAYALLWTIQERNALLERFLAL
                     EERIMPLLAKRTKEGFLLERDRLEMESWFSFARREAAAGSLLKEECLALIRKATGRKG
                     LFGLEALFPRLEPLFFSGDVLARAAGEENRELQILAKAAETKREIARRVPGAQYDAYL
                     RAGYGFSSEFPGRNGTEAFLSIAFDVPIREGRAASAAGRAALAGAEKAGHEMEARRLA
                     IVGDIRGGIARFDSAAAQRTFAMANVRTAAESVRAD"
BASE COUNT          338 a          429 c          521 g          365 t
ORIGIN      
        1 gccggctgga gccttctatc cggaacctgc ggccttgcgg gggtcttcat cggggcggcg
       61 ttcttcaagg gcatggatcc cctgctgccc gggctggccc tctcggggct ggttgccctc
      121 gcggggtttg ttgcaggaaa gaggaggccc ggtttcccgg aaagataaga ggcttccaga
      181 cgccatgtcc ggatcggaag gaccgcctgt cgggaaaaaa aggaatcccc gggtttcctg
      241 ggattggcga ggatttcctg tccagccatt cccagggatc tctttttgta ggaaaacata
      301 cgactttcac ggagaaagcg aatctgaata cgaacaggaa aattcaggga attattcgat
      361 gatccgtcac tcattttcga cgtatccata actgacagtt cgcccattga cacggtgtgt
      421 tttgtaatgt tataataatt tgagaaatgt ttcagggtgg tattttttaa agcctgattt
      481 gtgggaatgt tatgtgtatt taaagaaagt gcattgttca tttttgacgg ggagttttga
      541 catgaaaaaa atactgcttg cccttctggt gctttttttt ctccggggaa acagccgggc
      601 tgaactccgg atcggtcttc ccgacctcga gagggaactg acatcctctc ccgaggttct
      661 tgcggctgcg gcttccctgt ccgaaagcag ggccgttgaa gaacggcaga agatgaccgg
      721 gggtcccagg cttttcggcg aactctccct cggagggctg aatgagcccg cttcaagaag
      781 cgaaacgtct gccctggagg gctacacaaa agtgtgggcc aggggcggca tttccattcc
      841 tctcttcggc acctggcaaa aggagcgtat aagggagctg gaaaaccgga taatatccct
      901 tggggaggaa cttcgccttt ttgcggtccg gaaagcgaac ctcaccgctc tcaggaaggc
      961 ctatgccctg ctgtggacga tccaggagag aaatgcgctc cttgagcgct ttctcgctct
     1021 cgaggagcgg atcatgccgc tcctcgcaaa gaggacaaaa gaggggtttc ttctcgagag
     1081 ggatcgcctc gagatggaga gctggttttc cttcgcccgc agagaggctg ccgccggctc
     1141 tttgctgaaa gaggagtgcc ttgcccttat caggaaggcg acgggccgga aggggctttt
     1201 cggccttgag gcccttttcc cgcgccttga gcccttgttc ttttccgggg acgttcttgc
     1261 cagggccgca ggggaggaga accgggaact gcagattctc gcaaaggcag cggagacaaa
     1321 acgggagata gcgcgccggg tacccggggc ccagtacgat gcatacctca gggcgggtta
     1381 cggtttttcc tcggagtttc cggggaggaa cggtacggag gcatttctgt ccatagcctt
     1441 cgatgtcccc atcagggaag gtcgggcggc atccgccgca ggacgggccg ccctggccgg
     1501 ggcagagaaa gccgggcatg aaatggaagc gaggcgtctc gccatagtgg gggacatccg
     1561 gggaggaatt gcccggttcg actccgccgc tgcccagcgg acctttgcca tggccaacgt
     1621 ccggacagcc gcggagtccg tccgggcgga cag
//
LOCUS       DAOTHD010000539         1653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_87494_length_1653_cov_2.318523, whole genome shotgun sequence.
ACCESSION   DAOTHD010000539 DAOTHD010000000
VERSION     DAOTHD010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87494_length_1653_cov_2.318523"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="P5219_08935"
     CDS             <1..430
                     /locus_tag="P5219_08935"
                     /inference="COORDINATES: protein motif:HMM:NF016252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HRX26925.1"
                     /translation="PVKYPRTMAFAYRMRWLNPKEPLHSLGRAAATRMAAGNQEGTVR
                     FVLDFEGGELQDLPADAGLTSVVTLDDGARLLEKQLHKNGATGGWRLVLTVALDPEGK
                     FKTLFPTAPSRPRIRLSALLKKGENLPDPLTETWTYDLHP"
     gene            414..806
                     /locus_tag="P5219_08940"
     CDS             414..806
                     /locus_tag="P5219_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26926.1"
                     /translation="MTSIPETGMSTPAGSPDEWRYSRDRVLLYVRALDLPPFRGLDLA
                     VESLKAAGPASVSEAMRKLRELLAENGLDRGILDEEGRHISSMPSLNRGVMVAEPLDR
                     VPWKSALGRFLRRWRRNLFGPWETNGHA"
     gene            799..>1653
                     /locus_tag="P5219_08945"
     CDS             799..>1653
                     /locus_tag="P5219_08945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRX26927.1"
                     /translation="MHDAGTANPSWNRAASWRRILLLTLVFLPTLAAARTMAGLLPSR
                     GGTALEILLVAVFAVLFAWISVGFWTASMGFVLLLFKNNRFSVSRACEGMDLTIAGTD
                     ARTAVLIPIYNEDVRRVTAGLRTVWTSLGKTGSSGLFDFFLLSDTTDPDIWVEEEEAW
                     HRFCREENASGRVFYRRRSSNSRRKSGNVADFCRRWGKNYRYMIVFDADSVMAGTTMV
                     RMVQSMEARPDIGILQTPPAGVNRETLIARAQQFANHVYGPMFAAGLHFWQLGDAQYW
                     GHNAIIRTE"
BASE COUNT          293 a          563 c          515 g          282 t
ORIGIN      
        1 ccccgtgaag tatccccgga cgatggcctt cgcctaccgg atgcgctggc tgaacccgaa
       61 ggagcccctc cactccctcg gcagggctgc ggcgacccgg atggccgccg ggaaccagga
      121 agggacggta cgtttcgtcc tggatttcga gggcggagag ctccaggacc tgccggcgga
      181 cgcaggactg acgagcgtgg tcaccctgga cgatggggcc aggcttctcg agaagcagct
      241 tcacaagaac ggggccaccg gtggatggcg gctcgtcctc accgtcgccc tggatccgga
      301 ggggaaattc aagacccttt tccccactgc cccgtcgcgg cctcgcatcc gcctttccgc
      361 cctgctgaaa aagggagaga acctccccga ccccctcacg gagacgtgga cctatgacct
      421 ccatccctga gaccggaatg agcacccccg ccggatcccc cgacgagtgg cgctactcca
      481 gggacagggt gctcctctac gtccgcgccc ttgacctgcc gccctttcgg gggctcgacc
      541 ttgccgtgga gagtctcaag gcagccggac ccgcgtccgt gtcggaggcc atgagaaaac
      601 tcagggaact cctggcggaa aacggcctcg accgggggat cctcgacgag gagggaaggc
      661 acatctcctc catgccttcc ctgaacaggg gcgtcatggt ggcggagcct ctcgacaggg
      721 ttccgtggaa atccgccctt gggcgcttcc tccgccggtg gaggcggaac ctgttcggac
      781 cctgggagac aaacggccat gcatgacgcc ggaacggcca acccttcctg gaatcgcgcc
      841 gccagctgga ggcgcatcct gctcctgacc cttgtcttcc ttcccactct cgcagcggca
      901 cgcaccatgg ccggactgct tccctcccgg ggcggcaccg cactggagat cctcctcgtg
      961 gcggtgtttg ccgtgctttt cgcctggatt tccgtgggat tctggacggc atccatgggc
     1021 ttcgtcctcc tgctgttcaa aaacaaccgg ttctccgtgt cccgggcctg cgaagggatg
     1081 gatttgacca tcgccggcac cgatgcccgg accgccgtcc tcattcccat atacaacgag
     1141 gatgtcagac gggtgacggc ggggctccgc accgtctgga cctccctcgg gaaaacaggc
     1201 tcctccggtc tgttcgactt tttcctgctg agcgacacca ccgaccccga catctgggtg
     1261 gaggaggagg aagcctggca ccggttctgc cgggaagaga acgcctcagg gcgggttttc
     1321 taccggaggc gctccagcaa ctcgaggcgg aagagcggca acgtggccga cttctgccgc
     1381 cggtggggaa agaactaccg ctacatgatc gtcttcgacg ccgacagcgt catggcggga
     1441 accaccatgg tccgcatggt gcagagcatg gaagcccggc cggacatcgg catcctccag
     1501 acgcctccgg cgggtgtgaa cagggagacc ctcatcgcca gggcccagca gttcgcgaat
     1561 cacgtctacg gccccatgtt cgccgcaggc ctccatttct ggcagctcgg cgacgcccag
     1621 tactgggggc acaacgccat tatcaggacg gaa
//
LOCUS       DAOTHD010000540         1652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_87558_length_1652_cov_2.822167, whole genome shotgun sequence.
ACCESSION   DAOTHD010000540 DAOTHD010000000
VERSION     DAOTHD010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87558_length_1652_cov_2.822167"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1384
                     /gene="rny"
                     /locus_tag="P5219_08950"
     CDS             <1..1384
                     /gene="rny"
                     /locus_tag="P5219_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRX26928.1"
                     /translation="SKREKLTEAKEEIFRLRQEIEREGKERRNELQRAERRLEQKEEN
                     LDRKLDRVAHREEELKGRQEELEARLLEVEETKRKQMERHEEIAGMTRETARDLLLRQ
                     TEEDAQHILGLRLKELEEKAKREAERKAREIIVTAVQRCAVEHTSEAAVSVVDLPSDE
                     MKGRIIGREGRNIRTFETLAGVDLIVDDTPEAVTLSSFDPVRREIARLSLERLVTDGR
                     IHPARIEELIEKATRDVEEQIVEAGESALLETGIKNMHSELVHTLGQLKFRFSYGQNA
                     LQHSLEVAYISGMIAAELGLDEEIARRAGLLHDLGKAVDHQIEGPHALIGADLARRYN
                     ESPEIVNAIASHHEDQEAKSIYDVIVAAADAVSASRPGARRESLDAYVKRLEKLEELA
                     KGFNGVSKAYAIQAGREVRVVVSPNISDDGVVHKIAFDIARKIEEEMKYPGQIKVTVT
                     RETRATEYAK"
     gene            1392..>1652
                     /locus_tag="P5219_08955"
     CDS             1392..>1652
                     /locus_tag="P5219_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HRX26929.1"
                     /translation="MRILFIGDIMGSPGRKAVAAVLPLLRERCGGFDFVIANCENAAA
                     GKGITRKIADELFGLGIDGDFPGDSLPNCENEVKTAAPLPEEG"
BASE COUNT          408 a          483 c          531 g          230 t
ORIGIN      
        1 gagcaagcgg gaaaagctca ccgaggccaa ggaagagatc ttcaggctcc ggcaggagat
       61 cgagagggaa ggcaaggaac gccgcaacga actccagagg gcagagcggc ggctggaaca
      121 gaaggaagag aaccttgacc gcaagctcga ccgggtagcc caccgggagg aagagctcaa
      181 aggaaggcag gaggagctgg aagcccgcct gctcgaagtc gaggagacga agcggaagca
      241 gatggaacgc cacgaggaaa tcgccggcat gaccagggaa acggcccggg atctccttct
      301 ccggcagacc gaggaagatg cccagcacat tctcggactc aggctcaagg agctcgagga
      361 gaaggcgaag cgcgaggccg aacgaaaggc ccgggaaatc atcgtcaccg ctgtgcagcg
      421 ctgcgccgtg gaacatactt ccgaggcggc tgtgagcgtc gtcgatcttc cctccgatga
      481 aatgaaggga agaatcatcg gcagggaagg gcggaacatc aggaccttcg agaccctcgc
      541 cggagtggac ctcatagtgg acgacactcc cgaggcggtg accctcagca gctttgaccc
      601 cgtccggagg gaaatcgcca ggctttccct ggaacgcctc gtgaccgacg gcaggatcca
      661 ccctgcccgc attgaggaac tcatcgaaaa ggccaccagg gacgtggagg aacagatagt
      721 ggaagcgggc gaaagcgccc tgctcgaaac gggaatcaag aacatgcatt ccgagcttgt
      781 ccataccctc gggcagctca aattccggtt cagctacgga cagaacgcgc tccagcacag
      841 cctcgaggtc gcctacattt cgggcatgat cgccgcagag ctggggcttg acgaggaaat
      901 cgcccgccgc gccggactgc tgcacgacct cggcaaggcg gtggaccatc agattgaggg
      961 gccccatgcc ctcatagggg cggacctcgc cagacggtac aatgaatctc ccgaaatcgt
     1021 gaacgcaatc gcctcccacc acgaagacca ggaggcgaag agcatctacg acgtcatcgt
     1081 agccgccgcc gacgcggtca gcgcgtcccg cccaggggcc cgcagggaaa gcctcgatgc
     1141 atacgtcaaa cgccttgaaa agctcgagga gctggccaag ggcttcaacg gcgtgagcaa
     1201 agcctatgcg atccaggcgg gcagagaggt ccgggttgtg gtatccccga acatctccga
     1261 cgacggcgtg gttcacaaga ttgccttcga cattgcccgg aagatcgagg aggaaatgaa
     1321 gtatcccggc cagatcaagg tcaccgtcac gcgggagacc agggcgacgg aatacgcgaa
     1381 ataggcgaaa aatgaggata ctctttatcg gagatatcat gggaagcccc gggcgcaagg
     1441 ctgttgccgc ggtgcttccc ctcctccggg agcggtgcgg cggttttgac ttcgttatcg
     1501 ccaactgcga gaacgccgcc gccgggaagg gaatcaccag gaaaatcgcc gacgaactct
     1561 tcggcctcgg gatcgacggc gattttcctg gtgattccct tcccaactgc gagaacgaag
     1621 tcaaaaccgc cgcaccgctc ccggaggagg gg
//
LOCUS       DAOTHD010000541         1650 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_87728_length_1650_cov_2.415674, whole genome shotgun sequence.
ACCESSION   DAOTHD010000541 DAOTHD010000000
VERSION     DAOTHD010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87728_length_1650_cov_2.415674"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="P5219_08960"
     CDS             <1..1322
                     /locus_tag="P5219_08960"
                     /inference="COORDINATES: protein motif:HMM:NF018168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HRX26930.1"
                     /translation="GTWDLSDGVNAASSAASDLTLTDLQGFLTTSFGGEITATLNTAG
                     TQMTLSSPLNHLLSITDIKGGLVSALGMENSAPSVSIEVVEEDTLETIKNKINSAFSD
                     GENGLSRPEDWLHASIELDAATNTYFLSLESNVAGEAHRINVLGDENGSLQIAKRLGF
                     LNGDGSTNYREYARDAAFTFDGKLYLSESNMVRDARRVPVLNDYSAGTLEEVSPGIRL
                     ELRAVGESDITVKHHVTGGAIRGALEARDDIILNFLDVLDEMAYGLVKEMNALHYGGY
                     GVGESESVTGVAYFDPIAVQYGASRLFEINREIDEDRSLIAAASGDGNGFSNGSGDGS
                     NALRMAQLKQTKVLQSRSADFNEFYESFIASLGAQGDRAATMQKNQDTLISQITNQRQ
                     SVMGVNIDEEMMSIIKFQQAFNAMARYITTVDEMLDRIINGMGLVGR"
     gene            1335..>1650
                     /locus_tag="P5219_08965"
     CDS             1335..>1650
                     /locus_tag="P5219_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein 3"
                     /protein_id="HRX26931.1"
                     /translation="MQRVSNPMIQNLMLSDMHNNLSRLLEYQHQLATGKKHSRPSDNP
                     IDVVRELSLETAILENGQYIRNQDDAITWLSNTDTAFNQMMDIAHRIRELTIYAGNGA
                     LGE"
BASE COUNT          376 a          558 c          457 g          259 t
ORIGIN      
        1 ccggtacgtg ggatctctcc gacggcgtca atgcggcgtc gtccgctgcc tccgacctta
       61 cactgaccga cctccagggg ttcctcacca cgagtttcgg cggcgaaatc accgcgacgc
      121 tcaacactgc cggcacccag atgacccttt ccagtccgct gaaccacctt ctctccataa
      181 cggacatcaa gggcggcctc gtctccgccc tcggaatgga aaactccgct ccttccgttt
      241 ccatcgaagt ggtggaggag gataccctgg aaaccataaa gaacaagatc aacagcgcct
      301 tttccgacgg tgaaaacggc ctttccaggc ccgaggactg gctccatgca tccatcgagc
      361 tggacgccgc cacgaacacc tacttcctgt ccctcgaaag caacgtggcc ggggaggctc
      421 accggatcaa cgtcctcggt gacgaaaacg gcagcctcca gattgccaag cgcctcgggt
      481 tcctcaacgg cgacggcagc accaactaca gggaatatgc ccgggacgcc gccttcacct
      541 tcgacggaaa actctatctt tcggaaagca acatggtgcg ggacgccagg cgcgttccgg
      601 tcctcaacga ctactccgca ggcaccctcg aggaagtctc tccgggcatc cggctggagc
      661 ttcgggccgt gggcgaatcg gacatcaccg tgaagcacca cgtcacggga ggggccatca
      721 ggggcgccct cgaggcccgg gacgacatca tcctcaactt cctcgacgtc ctcgacgaaa
      781 tggcctacgg tctcgtcaag gagatgaacg ccctccatta tggcggctac ggagtggggg
      841 agagcgaatc cgtcacaggg gtggcctact tcgaccccat cgcggtccag tacggcgcga
      901 gccgcctgtt tgaaatcaac agggagatcg acgaggaccg cagtctcatc gcggccgcct
      961 cgggcgacgg aaacggcttc tccaacggct ccggggacgg cagcaacgcc ctccgcatgg
     1021 cccagctcaa gcagaccaag gtcctccaga gcagaagcgc ggacttcaac gagttctacg
     1081 agtccttcat cgcgagcctc ggcgcccagg gagaccgggc cgccaccatg cagaaaaacc
     1141 aggataccct catcagccag atcaccaacc agcgccagtc ggtcatgggg gtcaacatcg
     1201 acgaagaaat gatgagtatc atcaagttcc agcaggcctt caacgccatg gcacggtaca
     1261 taaccacagt cgacgagatg cttgaccgca tcatcaacgg catgggcctc gtgggccggt
     1321 agggagggag aaccatgcag cgcgtcagca accccatgat ccagaacctc atgctgtcgg
     1381 acatgcacaa caacctgtcc cggctgctgg agtaccagca ccagctcgcc acgggcaaga
     1441 agcattcgcg gccgtcggac aaccccatag acgtggtccg ggagctttcc ctcgagaccg
     1501 ccatactgga aaacggccag tacatccgaa accaggacga cgccatcacg tggctctcca
     1561 acaccgacac ggccttcaac cagatgatgg atatcgccca ccgcatccgg gagctcacca
     1621 tctacgccgg caacggtgcc ctcggggaag
//
LOCUS       DAOTHD010000542         1649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_87789_length_1649_cov_2.670640, whole genome shotgun sequence.
ACCESSION   DAOTHD010000542 DAOTHD010000000
VERSION     DAOTHD010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87789_length_1649_cov_2.670640"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..738)
                     /locus_tag="P5219_08970"
     CDS             complement(115..738)
                     /locus_tag="P5219_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26932.1"
                     /translation="MTFERGNEEKWTVGEILSAMAGTEHPRYIQKIVTQLQQWMEESL
                     TSETAEHDGDPLSALFDSVLEADGEKLFTQLEWVDDYADLLPNLKKPSGHEDAVVLNV
                     GPTDFEDSLRMAIDYASLFNRKLCRRVWMISDTFIIGDVYRYISHIRALGSQGIAFRF
                     LLVTPWGWTEIPLAADGTPGNRMIWNNRKGGAAPGEDERKRDDRLTR"
     gene            complement(828..1511)
                     /locus_tag="P5219_08975"
     CDS             complement(828..1511)
                     /locus_tag="P5219_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HRX26933.1"
                     /translation="MMLQAQKTGTAPLYEIRDLVCAYGDEPVLHIDRLDISSDGPTAF
                     VGHNGSGKSTLLKALAFLLKPAAGTIRFLGNSTEEREGELRREVTLLLQEPYLLRRSV
                     FENVAYGLRARGATDRLAERVAEALEMVGLSPDFGKREWFRLSGGESRRVALASRLAL
                     RTKVLLLDEPTANVDEENALLMARVIGEAREKWGTVSLIASHDIQWLDGVCGRRILMR
                     RGKIESISA"
BASE COUNT          299 a          476 c          526 g          348 t
ORIGIN      
        1 ctccgacctc ctggtcccga accaggcgcg cttaccagac tgcgctacgc cccgaaacac
       61 cgcgctgccg gagatggtat tccggcaacg gcgaagattg taacaccccc gggcctacct
      121 cgtcaaacgg tcgtcccgtt ttctttcgtc ttctcccggt gccgctccgc ctttccggtt
      181 gttccatatc atcctgttgc cgggcgtgcc gtcggcggcg aggggtattt ccgtccagcc
      241 ccagggggtg accaggagga accggaaggc gatgccctgg gagccgaggg cccggatgtg
      301 ggagatgtac cggtagacgt cgccgatgat gaaggtgtcg ctgatcatcc acaccctgcg
      361 gcagagcttc cggttgaaga gggatgcgta gtcgatggcc atgcggaggc tgtcctcgaa
      421 atcggtggga cccacgttga ggacgacggc atcctcgtgg cctgagggtt ttttcaggtt
      481 gggaaggaga tcggcgtagt catccaccca ctcgagctgg gtgaagagtt tctccccgtc
      541 cgcctcgagg acgctgtcaa agagcgcgga gagcggatcg ccgtcgtgtt cggcggtctc
      601 ggatgtgagc gattcctcca tccactgctg cagctgggtc actattttct ggatgtaccg
      661 tgggtgttcc gttcccgcca tggctgaaag aatctcccct accgtccatt tttcttcatt
      721 tccgcgttcg aatgtcatgg aatcctccgg aacttctatt atgggggaag cataacatat
      781 acccgttccc gccacaacaa aaagcaggga aagaaaatgg gcggttttca ggcggaaatg
      841 ctctcgattt ttccccgcct catgaggatg cgcctgccgc agacaccgtc gagccactgg
      901 atatcgtggg aggcaatgag ggaaactgta ccccattttt cccgggcttc cccgatcacc
      961 cgggccatga gaagggcgtt ttcttcgtcc acattggcgg tgggttcgtc gaggagaaga
     1021 accttcgtcc ggagggcaag tcgggaggcc agggccactc tcctggattc cccccccgag
     1081 agcctgaacc attctctttt tccgaagtcc ggggacaggc cgaccatttc gagggcttcg
     1141 gcgacccgtt cagcgagacg gtccgtggcc ccccgggccc gaaggccgta ggccacgttc
     1201 tcgaagacag agcgccggag gaggtaaggt tcctggagga ggagggtcac ttctctgcgg
     1261 agttcccctt cccgttcttc cgttgagttt cccagaaatc gtatggtgcc tgccgcaggt
     1321 ttcagaagga aggcaagggc cttcaggagg gtgctcttgc cgctgccgtt atggccgacg
     1381 aaggcggtgg gaccgtcgga ggaaatgtcc agccggtcga tatgcagtac cggttcgtct
     1441 ccataggcgc agacgaggtc cctgatctcg tagagagggg cagttccggt tttttgggcc
     1501 tgcagcatca tgatgccgtc ctcctgcgga gggccgccag ggaccagttg agaacgaagg
     1561 agagaccgag gagcaggacg ccgagggcga tgccggtgga gaattctccc ttgcccgtct
     1621 ccagggtgat ggcggtggtg atggtccgg
//
LOCUS       DAOTHD010000543         1648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_87859_length_1648_cov_2.558694, whole genome shotgun sequence.
ACCESSION   DAOTHD010000543 DAOTHD010000000
VERSION     DAOTHD010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87859_length_1648_cov_2.558694"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="P5219_08980"
     CDS             <1..464
                     /locus_tag="P5219_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRX26934.1"
                     /translation="TAKGDEVDRIVGLELGADDYLPKPFNPRELLARIRAILRRKESP
                     AAPNTPERLVSGDIELDPARRTIRAGGTDIDLTTVEFKLLEALLRTPGKVVSRDALSR
                     AALDRPLSPFERSLDVHMSNLRKKLGPCPDGSPRIRTLRSEGYLLACEATH"
     gene            446..>1648
                     /locus_tag="P5219_08985"
     CDS             446..>1648
                     /locus_tag="P5219_08985"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRX26935.1"
                     /translation="MRSYTLKLFWWFWLTAAVAVVASHMIGKLAEEEQFRNRGETMRE
                     IAALYGEKASSTLARNGREAALDYIAGLQRTIRITGYLFDDDGKEMLGQPVPDYIHAI
                     LDEARKKNGPASTFERGRPVFAVPLESFPGHYFAGALPNLFREKSSAATLYRLLAAVF
                     AAALLSWILAVRLTRPLKGLGKTMRKFAAGALDAREERAARRKDDIGTLARDFNAMAA
                     RIEELLKGQRRLLADVSHELRSPLTRLGVALELARRHIPEEAAGEYERMENELARMNA
                     LIGSLLQLSRLDALDRPENRQTMDLSALVGEIAEDGDFEGAPHGRGVSADILTGVSYS
                     GDPALLRSAVENLVRNALRYSPEGGTVDVSLSRQGGAVRILVEDRGPGVPDEELEHIF
                     TPFHRVEQA"
BASE COUNT          323 a          574 c          508 g          243 t
ORIGIN      
        1 tcaccgccaa gggggacgaa gtggacagga tcgtgggcct cgaactgggg gcggacgact
       61 atctgccaaa gcccttcaac ccccgggagc tcctggcccg catccgggcc atcctccgcc
      121 ggaaagagtc ccccgccgcc ccgaacaccc cggaacggct cgtctccggt gacatcgaac
      181 tggatcccgc ccggcggaca atccgtgcgg gaggaacgga catagacctc accacggtgg
      241 aattcaagct gctggaggcc cttctccgca ccccgggcaa ggtggtctcc cgggacgccc
      301 tctcccgggc cgccctggac cgccccctct cccccttcga gcggagcctg gacgtccaca
      361 tgagcaacct tcggaaaaaa ctcggcccct gccctgacgg atcgccccgg atcaggaccc
      421 tcaggagcga aggatacctc ctggcatgcg aagctacaca ctgaagctct tctggtggtt
      481 ctggctcacc gcagccgtgg cggtcgtcgc cagccacatg ataggcaagc tcgccgagga
      541 ggagcagttc cggaaccggg gggagaccat gagggaaatc gccgccctct atggcgaaaa
      601 agcctcctcc accctcgccc gaaacggcag ggaggcagcc ctggactaca tcgccgggct
      661 gcagcgcacc atacgcatca ccggctatct cttcgatgat gacgggaaag agatgctggg
      721 gcagcctgtc ccggactaca tccacgccat cctcgacgag gcccggaaga agaacggacc
      781 ggcgagcacc ttcgaacggg ggcggcctgt cttcgctgtt ccgctggagt ccttcccggg
      841 acactacttc gccggggccc tgccgaacct cttcagggaa aagagttccg ccgccactct
      901 ctaccgcctt ctcgcagctg tatttgccgc cgcccttctc tcgtggatct tggcggtcag
      961 gctcacccgg ccgctcaaag gactgggaaa aaccatgagg aaattcgccg ccggggccct
     1021 tgacgcccgg gaagagcggg cagcccgacg gaaagacgac atcggcaccc tggcacggga
     1081 cttcaacgcc atggccgcca ggatcgaaga actgctgaag ggacagcgga ggctccttgc
     1141 cgacgtgtcc cacgaactgc gctcccccct cacccgtctc ggcgtggccc tggaactcgc
     1201 gagaaggcac atcccggagg aagcggccgg agaatatgag agaatggaga acgagctggc
     1261 gcggatgaat gccctcatcg gctccctgct tcagctctcg aggctcgacg ccctcgacag
     1321 gcccgaaaac cggcagacca tggacctctc ggccctcgtg ggggagatcg ccgaggacgg
     1381 cgatttcgag ggggcccccc acggaagggg agtatccgcg gacatcctga ccggagtatc
     1441 ctattccggc gaccccgccc tgctccggag cgccgtggaa aacctggtcc gcaacgccct
     1501 ccgctactct cccgaagggg gaacggtgga cgtatccctt tcgagacagg gcggcgccgt
     1561 ccgcatcctc gtggaggaca ggggaccggg agtgcccgat gaggaactgg agcacatctt
     1621 cacccccttc cacagggtgg aacaggcc
//
LOCUS       DAOTHD010000544         1645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_88072_length_1645_cov_3.251572, whole genome shotgun sequence.
ACCESSION   DAOTHD010000544 DAOTHD010000000
VERSION     DAOTHD010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88072_length_1645_cov_3.251572"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="P5219_08990"
     CDS             complement(<1..687)
                     /locus_tag="P5219_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRX26936.1"
                     /translation="MRISTNLMSLASFTALSDVSNRLSKVSKKLATGLRINSASDDAS
                     GLAVSQKMRAQIRGLDRSAKNAQDGISLLQTAEGGIQEITSLLQRMRELSVQGANDVL
                     TSEDRGHIQGEIDELSSQVDAIANQTQFNRKKLLNGDMSALWSTSTSHLSVVMPGSGR
                     AGGTEEAAAVPSGNFRILFSTLDEGRENIQKSNILTLRNGTAGTGAVMDTGGAGLAAM
                     SALNLAEGSWR"
     gene            complement(944..>1645)
                     /locus_tag="P5219_08995"
     CDS             complement(944..>1645)
                     /locus_tag="P5219_08995"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRX26937.1"
                     /translation="SSLTAEKEKEIRFFRVNGISCAFLAYTTATNGLKAPEGKGHYVS
                     LYTPEKARNDVARAQKKADVVIVSMHWGSEYVFAPTAEQKKIAEHLASLGVSIVLGHH
                     PHVVQPVAMVKSTLVVYSLGNFLSSQKELHKLVGLLVSLDVVKTEMRGETRISFENVT
                     GTLLYNPRRSPLGRYVVVPFDMLREDILKNFSAVYKKYAAIVTGGGRVVSLRAPASPP
                     PAKPRSSGKKGRTSK"
BASE COUNT          309 a          488 c          498 g          350 t
ORIGIN      
        1 cctccagctc ccttcggcca ggttcagggc ggacatggcc gcaagcccgg cccccccggt
       61 gtccataacc gcaccggtcc ccgccgtccc gttcctgagg gtgaggatgt tgcttttctg
      121 tatgttttcc ctcccctcgt ccagggtgct gaagagaatc ctgaagttgc ccgacgggac
      181 ggcggcagcc tcttccgttc cgccggccct gccgcttccg ggcatcacta cggaaagatg
      241 ggatgtggac gtgctccaga gcgcggacat atcgccgttc agcaattttt tcctgttgaa
      301 ctgggtctga ttcgctatgg cgtccacctg ggaggagagt tcgtcgatct cgccctggat
      361 atgccccctg tcctcactgg tgagaacatc gttcgccccc tggacggaca gctctctcat
      421 ccgctgcaga agggaagtta tctcctgtat ccctccctcc gcggtctgaa ggagggatat
      481 gccgtcctgg gcattcttgg ccgaccggtc caatccccgt atctgggcgc gcatcttctg
      541 cgagacggcc aggcctgaag cgtcatcgga ggcactgttg atgcgaaggc cggtagccag
      601 tttttttgaa accttcgaca ggcggttact gacgtccgac agggccgtaa aagaggccag
      661 tgacataagg ttcgtggaga tccgcacttc cgggcacccc ccagaaagga ttcacagcgt
      721 atccgcaggg catgcccggc ctgaggaaaa gcctgcggga aagaacctgc atattggtat
      781 cggccggcag aagaaaaaac ctcagttttt ttggaaaaag aaaatacggg ccggcacatg
      841 agaattccct cccccggtac acccggggaa ggacaggagg gtacaaccgt ctggtaacag
      901 tgttttcagc ggccgggcag cagtccggac aatgctcttc agctcatttt gatgtccggc
      961 cttttttccc ggaagaccgg ggtttcgccg gaggcgggga agccggcgcc ctcagagaga
     1021 caaccctgcc gccgcccgtc acgatggcgg cgtacttttt atacaccgct gaaaagttct
     1081 tcaggatatc ctccctgagc atgtcgaagg gaaccaccac gtaccgcccc aggggagacc
     1141 ggcggggatt gtagagcagc gtcccggtga cgttctcgaa ggagatccgg gtctcccccc
     1201 tcatttccgt cttgaccacg tcgagggaga cgagaaggcc cacgagcttg tggagctcct
     1261 tctgggacga aaggaaattg ccgagggaat agaccacgag ggtgctcttc accatggcca
     1321 cgggctggac cacgtgggga tggtggccga ggacgatgga aacgccgagg gaggcgaggt
     1381 gctccgcgat ttttttctgc tccgcggtgg gtgcgaagac atattcgctt ccccagtgca
     1441 tggagacgat caccacgtcg gcttttttct gcgcccgggc cacgtcgttc ctggcttttt
     1501 ccggcgtgta gaggctgacg taatgtccct ttccctccgg ggccttcagt ccgttggtgg
     1561 cggtcgtgta ggccaggaag gcgcaggaaa tgccgttgac ccggaagaac ctgatttctt
     1621 tttccttctc cgcggtcagg ctgct
//
LOCUS       DAOTHD010000545         1645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_88076_length_1645_cov_3.157862, whole genome shotgun sequence.
ACCESSION   DAOTHD010000545 DAOTHD010000000
VERSION     DAOTHD010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88076_length_1645_cov_3.157862"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="P5219_09000"
     CDS             <1..323
                     /locus_tag="P5219_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26938.1"
                     /translation="TNVVIERFPEGGGSRFFVILRSVFCDDRAASPKGEELGVAFDFA
                     QLRDPSHSLGMTIPEGAFCPIASPKGKELGVDSEDPKTRSFTSFRMTNLLSSRGGASP
                     EGRI"
     gene            551..>1645
                     /locus_tag="P5219_09005"
     CDS             551..>1645
                     /locus_tag="P5219_09005"
                     /inference="COORDINATES: protein motif:HMM:NF025774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HRX26939.1"
                     /translation="MPLQDLLNQAVEVRPGDHLVALYAEEKEIEDYVTSFIHSALLRN
                     ERCLYITGDVDSSAVLQRVERLSAGSGASGELLVLEKSDVYSKGGKFSPDKLISLIQS
                     TVETAVREGYSGLAITGEISWVLDYEDGEDLIIEYEWKLNEYVFDRYPVSALCRYNVN
                     RFSHEMIRNIIQLHPLILWRSRIHENPYYIPPEGFKNNAIAQYQVDTWLKNIFSFTNT
                     KSRFQSIVDRKQEEMRLLHKNMTNGIIMAFLKLLETHDPYTKDHCSNVASLAFRLAES
                     LEISEEFSTKIHYASLVHDIGKTIVPHGILNKPGNLTGEEYGYIKMHPEHGANALDQM
                     GQVREIAQAVRHHHERYDGRGYPDGLSGDEI"
BASE COUNT          412 a          429 c          452 g          352 t
ORIGIN      
        1 taacgaacgt agtgatcgag agattccccg aagggggagg atctcggttt tttgtcatcc
       61 tgaggagcgt cttctgcgac gatcgcgcag catccccgaa gggggaggag ctcggggttg
      121 cctttgattt tgctcaactg cgagatccct cgcattcgct cgggatgaca atccctgagg
      181 gtgctttttg cccgatcgcc tccccgaagg ggaaggagct cggggttgat tctgaagacc
      241 ctaagaccag atccttcact tcgttcagga tgacaaatct cctgtcatcc cgaggcggag
      301 catccccgga ggggaggatc tgatttaaag gtcttaaaga ccgaaccgag attcttcggg
      361 cttcgccctc agaatgacac ctcctcccga agaggaagca tctgcagttg agacaacatc
      421 agagcgcgag agacccataa tcagaaaatt tcctgtatta tggtggtgtt tcataacgtc
      481 acgttccctt gacgagaaat cggatataat cactgaaatc tgagtgaaga gagaacgggg
      541 ggaagtcttc atgcccttgc aggatctgct gaaccaggcc gtggaagtgc ggccggggga
      601 ccatctggtc gcactctacg ccgaagagaa ggaaatcgag gactatgtga cttcattcat
      661 ccattcggcc ctcctgcgga atgagcggtg tctttacatc acgggggacg tggattcttc
      721 cgccgtgctt cagcgggttg aaagactctc agccggttcc ggcgcctccg gagaacttct
      781 cgtgctcgaa aaatcggacg tgtattcgaa aggcggcaaa ttttctccgg acaagctgat
      841 ctcgttgatc cagtcgacgg ttgaaaccgc agtcagggaa ggctacagcg ggcttgccat
      901 aactggcgag atcagctggg ttctggatta cgaagacggc gaggatctca tcatcgagta
      961 cgagtggaag ctgaatgagt acgtctttga cagataccct gtttccgccc tgtgccggta
     1021 taacgtaaac aggttttccc acgagatgat ccggaacatt atccagctcc atccccttat
     1081 cctctggcgg agcagaattc acgaaaaccc ctattacatc ccgccggaag gcttcaagaa
     1141 caacgccata gcacagtacc aggttgatac ctggctgaaa aacattttca gttttaccaa
     1201 tacgaaaagt cggttccaga gcatcgtgga cagaaagcag gaggaaatgc gcctgcttca
     1261 caagaacatg acgaacggca tcatcatggc cttcctgaag ctcctggaaa cccatgaccc
     1321 ctacacaaag gaccactgtt cgaacgttgc ctccctggcg tttcggctgg cggaaagtct
     1381 cgaaatatcc gaggagttca gtacgaaaat ccactacgca tcccttgtcc atgatatcgg
     1441 gaagaccatt gtcccccacg gcatcctgaa caagcccgga aatctgacgg gggaggaata
     1501 cgggtacatt aagatgcacc cggaacacgg agcgaacgct ttggatcaaa tgggccaggt
     1561 gcgggaaatc gcgcaggcgg tccgacatca tcatgaacgg tatgacggcc ggggataccc
     1621 cgacggactg tctggggacg agatc
//
LOCUS       DAOTHD010000546         1642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_88352_length_1642_cov_2.001260, whole genome shotgun sequence.
ACCESSION   DAOTHD010000546 DAOTHD010000000
VERSION     DAOTHD010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88352_length_1642_cov_2.001260"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..238
                     /locus_tag="P5219_09010"
                     /pseudo
     CDS             1..238
                     /locus_tag="P5219_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012778787.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            269..1513
                     /gene="guaB"
                     /locus_tag="P5219_09015"
     CDS             269..1513
                     /gene="guaB"
                     /locus_tag="P5219_09015"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: protein motif:HMM:NF012690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRX26940.1"
                     /translation="MSIEKQAREVDVVKRSESGVIVDPFFLHPEDMVSEAIGLMEHFH
                     ISGVPIVDGGKKLVGIITNRDLRFVTDYEQPISRVMTKENLITAPEGTTLADAQSILM
                     KYKVEKLPIVDGNGTLKGLITIKDIQKAKDFPNAAKDGGGRLRVGAAVGVGTDLAERA
                     AALVKSGVDVIVVDTAHGHSKKVLDSVRMLRTSYPDLVIIGGNIATAEAGEALIEAGV
                     DAVKVGVGPGSICTTRIIAGIGVPQLAAIYNVAQAAHKKGKTVIADGGIRFSGDIVKA
                     LAAGADSVMIGSLFAGTEESPGELIIYRGRSYKSYRGMGSLGAMKDGCSKDRYFQEGA
                     GNDKLVPEGIEGLAAHKGALSGVVFQLTGGLRSGMGYVGAKDIPDLQEKARFVRISAA
                     SVKENHPHDVVVTKEAPNYWVE"
BASE COUNT          394 a          453 c          505 g          290 t
ORIGIN      
        1 cgacctgctc tcagctgaag tcaacgagaa gcaggcgcgc tcaatcaaat accagctcac
       61 catcgccaag ctgccgctcg ccaaagacct caccgacttc aagttcgccg gcacgccgat
      121 caacgagacc ctggtcaagg acctcgccgg cggcggcttc atcgcccagc aacgcaatgt
      181 tgtcctgatc ggcggaaccg gaactggcaa gacgcacctg gccatcgcca tcgccaggga
      241 gggaggaata ggcatcgtcc acaggaatat gtccatcgag aaacaggcca gggaagtgga
      301 cgtggtgaag cggtccgaat cgggcgtcat cgtcgatccc tttttccttc atccggagga
      361 catggtgagc gaggccatcg gtcttatgga gcatttccat atttccggcg tccccatcgt
      421 tgacggaggg aaaaaactcg tgggcatcat caccaacagg gatcttcgct tcgtcaccga
      481 ctacgaacag cccatttcaa gggtgatgac caaggaaaac ctcatcaccg ctccggaagg
      541 gaccaccctg gccgatgcgc agtccatcct catgaaatac aaggtggaga agctccccat
      601 cgtggacgga aacggcacgc tgaaaggcct tatcaccata aaggacatcc agaaagcgaa
      661 ggacttcccc aacgcggcca aggacggagg gggaaggctc cgggtcggcg ccgccgtcgg
      721 cgtgggcact gacctggcgg aaagagcagc cgcccttgtg aagagcggag ttgacgtcat
      781 cgtggtcgat acagcccacg gtcattcaaa aaaagttctc gattccgtca ggatgctccg
      841 gacatcctat ccggatcttg tgattatcgg gggcaacatc gcaacggcgg aagcggggga
      901 agccctcatc gaggctggag tagacgccgt caaggtcggc gtcggacctg ggagcatctg
      961 caccaccagg atcattgcgg gaatcggcgt tccccagctt gcggcaatct acaacgttgc
     1021 ccaggctgcc cataaaaagg gaaagacggt aatcgccgac ggcggaatcc gcttttccgg
     1081 agacatcgtg aaagcgctcg ctgcgggggc agactcggtc atgatcggct cgctttttgc
     1141 ggggactgaa gaaagcccgg gggaactgat catttaccgg gggcggtcct acaagagcta
     1201 cagggggatg ggatccctcg gggctatgaa ggacggttgc agcaaggaca ggtatttcca
     1261 ggaaggggca ggaaatgata aactggtacc cgaggggatc gagggacttg ccgcccacaa
     1321 aggagccctg tcgggagtgg tcttccagct tacagggggt ctgcggtccg gtatggggta
     1381 cgtgggcgcg aaggatatcc cggaccttca ggaaaaggcc aggtttgtga ggatttcagc
     1441 agcctccgta aaggagaatc atccccatga cgtggtggtg acaaaggaag ccccgaacta
     1501 ctgggttgag taatcccccg gccggaaagg aggggatctt tccatgcagg gagcgaaggt
     1561 ttttgaacgg aacatattcg gggacgaaca ctcccagcca ggagtgttcg tccccgaata
     1621 tgttccgttc aaaaaccttc gc
//
LOCUS       DAOTHD010000547         1641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_88417_length_1641_cov_2.659521, whole genome shotgun sequence.
ACCESSION   DAOTHD010000547 DAOTHD010000000
VERSION     DAOTHD010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88417_length_1641_cov_2.659521"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="P5219_09020"
     CDS             <1..493
                     /locus_tag="P5219_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HRX26941.1"
                     /translation="GISDGGMNTYPDLNAKAKIIENAVACYHKIGVPQPRIAALAAVE
                     AVNPDMQATLDAAALAKMNERGQIRGCIVDGPLALDNAVSEESAKIKGISSPVAGEAD
                     MLLVPDIESGNFVGKVLLYMTGGKGAGVILGARKPIVLTSRFDSMETKLLSIALGAVV
                     ARS"
     gene            530..1459
                     /locus_tag="P5219_09025"
     CDS             530..1459
                     /locus_tag="P5219_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HRX26942.1"
                     /translation="MDQIRSLSQLLEYAKKIGAEKGPKKLSVAMAEEAGLLSAIEEAR
                     QAGFAEAILVGNADKIKAAADQAGVNLANYEVIDERNEPAMGMTAVSQVSSGKADIYM
                     KGQIHTNNFLRAMLNKEVGLRKGKNTISHCYFHSVEGYDRIFFVSDAAFNMYPDLAAK
                     ADITQNTVNLARAFGVAEPKVAVLAAVEVVNPDMPATLDAAALTQMNRRGQIKNCVVD
                     GPFALDNAVSEESAKTKGIVSPVAGKADVFIVPNIDGGNMLAKAIVYFSKNETAGLIL
                     GAAAPIVLTSRADSARAKLLSIAAAVTLSDFQK"
     gene            1475..>1641
                     /locus_tag="P5219_09030"
     CDS             1475..>1641
                     /locus_tag="P5219_09030"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520896.1"
                     /note="catalyzes the phosphorylation of 2-butanoate to
                     butanoyl phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HRX26943.1"
                     /translation="MKVLALNPGSTSTKVALFEDGTELWSETQRYDTDVIGKFASIPD
                     QEGFRLDEIRK"
BASE COUNT          358 a          529 c          493 g          261 t
ORIGIN      
        1 cggcatctcc gacgggggga tgaacaccta ccccgacctg aacgccaagg cgaagatcat
       61 agaaaacgct gttgcgtgct atcacaagat cggcgttccc cagcccagaa tcgccgccct
      121 ggcggcagtg gaggccgtca accccgacat gcaggccacc ctcgatgcgg ccgctcttgc
      181 gaagatgaac gagcgggggc agatccgggg ctgtatcgtg gacggccccc tggcgcttga
      241 caacgccgtc agcgaggagt cggcgaagat caaggggatc agctccccgg tggccggaga
      301 agccgacatg ctcctggttc ccgacatcga gtccggaaac ttcgtcggaa aggtgctgct
      361 ctacatgacg ggaggcaagg gcgcaggggt catcctgggg gcccggaagc ccatcgtcct
      421 caccagccgt ttcgacagca tggagaccaa gctgctttca atcgccctgg gcgccgtggt
      481 ggccaggagc tgacacattc agacccaatt tcagaggagg aattgcatta tggatcagat
      541 ccgttcactg tcacagcttc ttgagtacgc gaagaaaatc ggcgccgaaa aggggccgaa
      601 gaagctttcc gtggccatgg ccgaagaggc cgggctgctg tccgccatcg aggaagcccg
      661 gcaggcaggg tttgccgagg ccattctcgt gggcaacgcc gacaagatca aggccgccgc
      721 tgaccaggcc ggcgtgaacc tcgccaacta cgaggtcatc gacgagagga acgaacccgc
      781 catgggcatg accgccgtct cccaggtctc atcgggaaaa gccgacatct acatgaaggg
      841 gcagatccac acgaacaact tcctccgggc catgctcaac aaggaagttg gtctccggaa
      901 gggcaagaac accatctccc actgctactt ccacagcgtt gaaggatacg acaggatctt
      961 cttcgtgtcc gacgccgcct tcaacatgta ccccgatctg gctgccaagg ccgacatcac
     1021 ccagaacacg gtgaacctcg ccagggcatt cggcgtggcc gagcccaagg tggccgttct
     1081 cgccgccgtg gaagtggtga accccgacat gcccgccacc cttgacgccg cggcccttac
     1141 ccagatgaac cgccggggcc agataaagaa ctgcgtcgtg gacggcccct tcgcccttga
     1201 caacgccgtg agcgaagaat cagcgaagac caagggcatc gtctcgcccg tggccggcaa
     1261 ggcggacgtc ttcatcgtcc ccaacatcga cggggggaac atgctcgcca aggccatcgt
     1321 ctacttctcg aagaacgaga ccgcagggct catcctcggc gcggccgcgc ccatcgtgct
     1381 caccagcagg gccgactccg ccagggcgaa gcttctctcc atcgccgccg ccgtcaccct
     1441 gtccgatttc cagaagtaag acggcctgcc ggtcatgaag gttcttgcgc tcaaccccgg
     1501 ctccacgagc acgaaggtcg ccctcttcga ggacggtacg gagctgtgga gcgaaacaca
     1561 gcggtacgac acggacgtca tcgggaaatt tgcctccatt ccagaccagg aggggttccg
     1621 gcttgacgag attcggaaat g
//
LOCUS       DAOTHD010000548         1640 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_88506_length_1640_cov_2.436593, whole genome shotgun sequence.
ACCESSION   DAOTHD010000548 DAOTHD010000000
VERSION     DAOTHD010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88506_length_1640_cov_2.436593"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="P5219_09035"
     CDS             <1..312
                     /locus_tag="P5219_09035"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRX26944.1"
                     /translation="FLALMDTEELCVCDVGDFLGLAPATVSRHMSILRQAGLIESEKR
                     GRWVYYRMGTAVDPRLLGWIRSSVGNDGFPAQDREKIREIVRLHRESCLCDSGERKTT
                     V"
     gene            309..752
                     /locus_tag="P5219_09040"
     CDS             309..752
                     /locus_tag="P5219_09040"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HRX26945.1"
                     /translation="MKNKLKVLFLCTGNSCRSQMAEGWTRHLHGDVVEAWSAGIETHG
                     LNPNAVAVMKEAGVDISGHRSKNVAELLDVPFDFVITVCGDADERCPFFPGNARIIHR
                     GFDDPPKLALGKETEEEKMDCYRRVRDEIRAFLEDLPGFLENLPE"
     gene            772..>1640
                     /gene="arsM"
                     /locus_tag="P5219_09045"
     CDS             772..>1640
                     /gene="arsM"
                     /locus_tag="P5219_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HRX26946.1"
                     /translation="MDDIRNYVKERYARAIKNNTSCCGSGGCCCGTTGASDILSMTRG
                     NYDGTVLEKTPPQMADQSFGCGNPFTEADIHPGETVLDLGSGAGLDLFLASEATGPSG
                     KVIGLDMTDEMLETAAENLRGLDNVTLVKGYIEEMPLESGTVDVIISNCVINLSPDKG
                     KVLKEAFRVLRPGGRFCVSDTVFLRPVTERAVKNLAAWSGCIAGALQETEYADLLKEA
                     GFIEIEVRRTKVYDVPDALAAMAFPELSAEERKEINGALASALILAKKTRTVPLGKNG
                     PGKSNRKRGLGDEG"
BASE COUNT          368 a          467 c          529 g          276 t
ORIGIN      
        1 ttcctggcgc ttatggatac ggaagaactg tgcgtctgcg acgtggggga cttcctcgga
       61 cttgcgcccg ccacggtttc ccgccacatg agcatcctcc gccaggcagg gctcatcgaa
      121 tcggaaaaaa gggggcggtg ggtttactac cgcatgggaa cagccgtcga tccccggctg
      181 ctcgggtgga tacggagctc cgtggggaat gacggctttc cggcgcagga cagggagaaa
      241 atcagggaaa tcgtcaggct gcacagggag agctgcctct gtgactcagg ggagaggaaa
      301 acgaccgtat gaagaacaag ctgaaggttc tgtttctctg taccggaaac tcgtgccgca
      361 gccagatggc ggaaggctgg acccgccatc ttcacggaga cgtcgtagag gcatggtcag
      421 cggggattga aacccatggc ctgaacccca atgccgtggc ggtgatgaaa gaagccggag
      481 tggacatctc cgggcacagg tcgaaaaacg ttgccgaact gcttgacgtt cctttcgact
      541 tcgtcatcac cgtatgcggc gacgccgatg aacggtgccc cttcttcccg ggaaacgcac
      601 ggatcatcca ccggggattc gacgaccctc cgaagctcgc cctggggaag gaaacggagg
      661 aggaaaaaat ggactgctac cggcgggtcc gggacgaaat acgcgcgttt ctagaggacc
      721 ttcccggctt tctggaaaac cttcctgaat agaaaaggag ctggactgac aatggacgac
      781 atccgcaact acgtgaaaga acggtacgcc agggccatca aaaacaatac ttcctgctgc
      841 ggctcgggag ggtgctgctg cggcacgacg ggcgcatccg acatcctgag catgacccgg
      901 ggcaactacg acggcacggt gctcgaaaag acgcccccgc agatggcgga ccagtccttc
      961 gggtgcggca atcccttcac cgaagccgac atccatccgg gggagaccgt cctcgacctt
     1021 ggaagcggcg cgggtctcga tcttttcctg gcctcggaag ccacgggacc ctccgggaag
     1081 gtcatcggcc tcgacatgac cgacgagatg ctcgagaccg ccgccgaaaa tctcaggggg
     1141 ctggacaacg tcaccctcgt gaagggctac atcgaggaaa tgccgctgga gagcggcacc
     1201 gtggacgtga tcatatccaa ctgcgtcatc aacctctccc ccgacaaggg gaaggtgctg
     1261 aaggaggcct tccgggtgct ccgaccgggc ggccggttct gcgtctccga cacggtgttt
     1321 ctccgtcctg tgacggagcg ggccgtgaag aacctcgccg cgtggtcggg gtgcatcgcc
     1381 ggggctctgc aggagacgga gtacgccgat cttctgaagg aagcgggatt cattgagatc
     1441 gaagtccggc gcacgaaggt ctacgatgtg cctgacgctc tggccgccat ggcatttccc
     1501 gaactctcgg ccgaagaacg gaaggagatc aacggtgcac tggccagcgc cctgattctg
     1561 gcgaaaaaaa ctcggactgt ccccctgggg aaaaacgggc ccgggaaaag caataggaaa
     1621 agaggtctgg gagatgaagg
//
LOCUS       DAOTHD010000549         1639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_88581_length_1639_cov_2.472854, whole genome shotgun sequence.
ACCESSION   DAOTHD010000549 DAOTHD010000000
VERSION     DAOTHD010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88581_length_1639_cov_2.472854"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="P5219_09050"
     CDS             <1..294
                     /locus_tag="P5219_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HRX26947.1"
                     /translation="HPLQAVLDYPAAFGVLGLAGFFAGRPLAGMVLSGLLRFGCHVLS
                     GVIFFGSFAPEGSNVWLYSSVYNGSFLLPTLVVSAVLVHLILPRLPAFGTGRR"
     gene            complement(251..1354)
                     /locus_tag="P5219_09055"
     CDS             complement(251..1354)
                     /locus_tag="P5219_09055"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRX26948.1"
                     /translation="MTKKKLFDSRRDGEQATSALLDWYDLVRRPFPWRGTTDPYKIWI
                     SEVMLQQTQTSRAADYYLRWTGLFPTAEALARAPEEDVLKAWEGLGYYGRARNLLAAA
                     KVIAGTLGGRLPESYEGLLALPGVGPYTAGAVASIAFGLPVPAVDANGKRVFSRLLDL
                     ENPVDRSAGEETIREAFALLLSHDRPGDFNQAVMELGATLCLPRSPKCGKCPLSGICR
                     ARERGTVLLRPVFSGKEKGETAEGKMIVLLREGKAFLRKRPSRGLWAGFEEFPWEAPP
                     AEPPLPSALSHAEGADFGLIRCSFTRWRITLRVVIVPAQDGMEQNTPGGRWAGGDELA
                     SLPLPSGSARVRKMLFRAGLIACPSRRPEAGAE"
     gene            1454..>1639
                     /locus_tag="P5219_09060"
     CDS             1454..>1639
                     /locus_tag="P5219_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26949.1"
                     /translation="MTGAEKTEYDFSLFGEMDSYLFREGNHCRLYEKLGAHVTEHGGK
                     RGTYFAVWAPNAEEVSVT"
BASE COUNT          283 a          502 c          523 g          331 t
ORIGIN      
        1 catcccctcc aggcggtgct cgactatccg gcggccttcg gcgttctcgg cctggccgga
       61 tttttcgcgg gcaggcctct ggccggaatg gttctttcgg ggcttctgcg gttcggatgc
      121 catgtccttt ccggcgtgat ctttttcggc agctttgccc ccgagggaag caacgtgtgg
      181 ctctattcct cggtctacaa cggctccttc cttcttccca ccctggttgt gtctgccgtg
      241 ctggtgcatc tcattctgcc ccggcttccg gccttcggga cgggcaggcg ataagccccg
      301 ccctgaagag cattttccgt accctggcgc tcccggaggg gagaggaagg gaggcgagtt
      361 cgtcgccccc ggcccatctc cctccgggag tgttctgttc catgccgtcc tgagcgggga
      421 cgatcaccac ccgaagggtg atgcgccacc gggtgaagga gcaccggatc aggccgaaat
      481 cggccccttc cgcatgagaa agcgcagagg gcaggggagg ttccgccggg ggtgcttccc
      541 aggggaactc ttcgaaaccg gcccagagcc cccgggatgg gcgcttccgg agaaaggctt
      601 ttccttccct gaggaggacg atcattttcc cttccgcggt ctctcccttt tcttttcccg
      661 aaaagaccgg gcggagcagg accgtccccc gttcacgggc ccggcagatc cctgaaaggg
      721 ggcattttcc gcacttgggc gaccggggga ggcagagggt ggctcccagt tccatcaccg
      781 cctggttgaa gtcaccgggg cggtcatggg agagtaggag ggcaaaggcc tcccggatgg
      841 tctcttctcc ggcggaacgg tccacggggt tttcgaggtc gagaaggcgg gagaacaccc
      901 tttttccgtt ggcgtccacc gccggaacgg gaaggccgaa ggcgatgctc gccaccgctc
      961 cggcggtgta ggggccaacg ccgggaaggg caagaagtcc ttcgtaggac tccggaagcc
     1021 tgcctccgag tgtgccggcg atcaccttcg ccgccgccag gaggttccgt gccctgccgt
     1081 aatagcccag gccttcccac gccttgagaa cgtcctcctc tggggcccgg gcgagggctt
     1141 ccgccgtggg aaaaaggccg gtccaccgga ggtagtagtc cgccgcccgg gatgtctggg
     1201 tctgctgcag catcacctcg gaaatccata ttttgtacgg gtcagtggtc cccctccagg
     1261 gaaaggggcg ccggaccagg tcataccagt ccagaagggc tgatgtcgcc tgctctccgt
     1321 ctcgccggga atcgaagagt ttctttttcg tcatgaaata actccttcgg tgattttggg
     1381 aaaacggttc tctccgccgt catagggtac aataactttc agattgatgc accacctggg
     1441 ggaggagaga accatgaccg gagcagaaaa aacggagtat gatttttctc ttttcggaga
     1501 gatggactcg tatctcttcc gggaggggaa ccattgccgc ctctacgaaa aactcggcgc
     1561 ccacgtcacg gaacacggcg ggaagcgggg aacatacttt gccgtctggg ctcccaacgc
     1621 cgaggaagtg agcgtgacc
//
LOCUS       DAOTHD010000550         1635 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_88860_length_1635_cov_3.479114, whole genome shotgun sequence.
ACCESSION   DAOTHD010000550 DAOTHD010000000
VERSION     DAOTHD010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88860_length_1635_cov_3.479114"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="P5219_09065"
     CDS             <1..819
                     /locus_tag="P5219_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HRX26950.1"
                     /translation="LWTSVPAFLISLAVYGYVGSNASGEIDLTSVNEILGGISKTFKM
                     TPLVLVPPLAVLVLAYRRYPTLPVLWIAIVLAVPLAMMQGYDLTTIVKSMASGPKIVT
                     EVASLDKLLSRGGLAFMSGSVVVVFFAYIFAGQLEYTGTFRKICSSLKESFIGNSKGR
                     FVVSASVTGILTGLGTGNSYLSEIVPGTMYKDLADEMDISRRVLSRTLEDSGTVVVPL
                     IPWSAAGIYMSTVLGVSVFEYVPWAFLCYLGFLTAWFYGFTGIAIWPSDKKKAA"
     gene            955..>1635
                     /locus_tag="P5219_09070"
     CDS             955..>1635
                     /locus_tag="P5219_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HRX26951.1"
                     /translation="MLLIRNADLYDPEHKGLCDILMAAGRILSVYSAGTADPGALSSL
                     SADFRTVDAGGNLTMPGILDRHVHFNGAGGEGGPANRTPPLQLSSFVRAGVTSAVGML
                     GTDGTCRALEELLMKAKGLEEEGISTWILTGSYGLPSPTLTGSVARDLCLIDKVIGLK
                     LAVSDHRSSHPSVEEIRRAASNVRVGGILSGKAGCLCVHMGSEASGLTPLLDAMEGTD
                     IPITQFAPT"
BASE COUNT          270 a          552 c          486 g          327 t
ORIGIN      
        1 ctctggactt cggtccccgc attcctcatc agcctcgcgg tgtacggcta cgtgggatcg
       61 aacgcctccg gcgagatcga ccttacgtcc gtcaatgaaa ttctcggcgg catttccaag
      121 accttcaaga tgacccccct cgtgctggtt ccgcccctgg cggtgctggt gctcgcctac
      181 cggcgctatc ccacccttcc tgtgctgtgg atcgccatcg tccttgccgt gccccttgcc
      241 atgatgcagg gctatgacct caccaccatc gtgaagtcca tggcctccgg tccgaagatc
      301 gtcaccgagg tggcgagcct ggacaagctc ctgagcagag gcggtctggc cttcatgtca
      361 ggttcggtgg tggtggtctt cttcgcctac atcttcgccg gccagcttga gtacacgggc
      421 accttccgga agatctgctc ctccctgaag gagagcttca tcggcaacag caagggacgg
      481 ttcgtcgtgt ccgcgtccgt cacgggcatt ctcacgggcc tcggcacggg aaattcctac
      541 ctgagcgaaa tcgtcccggg caccatgtac aaggaccttg ccgacgagat ggacatctcc
      601 cggcgggtgc tctccagaac cctcgaggac tccggcaccg tggtggtgcc cctcattccc
      661 tggtccgctg cgggcatcta catgtccacc gttctcggag tgtccgtctt cgagtacgtg
      721 ccctgggcct tcctgtgcta cctcggcttc ctgacggcct ggttctacgg cttcaccggc
      781 atcgccatct ggccgtctga caaaaagaaa gccgcctgat ccatccggat ccccggggag
      841 ccccggctcc ccggggaccg ttgcccttcc ccgtaccttc cggtacactg acttgaggag
      901 aataagctta tcttcccccg ccaaggcggg acggaggaaa ggagaaccta ttgcatgctt
      961 ctgattcgga acgccgacct gtatgaccct gaacacaagg ggctgtgcga cattcttatg
     1021 gccgccggcc gcattctttc agtatattcc gccggaacgg cggatccggg ggccctgtca
     1081 agcctctccg cggatttcag gacggtggac gccgggggaa acctcaccat gccgggtatc
     1141 ctcgacaggc acgtccactt caacggtgcc gggggcgaag ggggaccggc caacaggacc
     1201 ccgcccctcc agctttcctc ctttgtccgg gcgggggtca cctcagccgt ggggatgctg
     1261 gggaccgacg gcacctgccg tgccctggag gagctcctga tgaaggcaaa ggggctcgag
     1321 gaagaaggca tttccacgtg gatcctcacg gggagctacg gacttccctc gccgaccctc
     1381 acgggttccg tggcgaggga cctctgcctc atcgacaagg tgatcggcct caagctggcc
     1441 gtgtcggacc atcggagttc ccacccctct gtggaggaga tccggcgggc cgcctccaac
     1501 gtccgggtgg ggggcatcct ctccggcaag gcaggctgcc tctgcgtcca catggggagt
     1561 gaggcctcgg ggctgacgcc tcttctcgac gccatggagg gaacggacat ccccatcacc
     1621 cagtttgctc ccacc
//
LOCUS       DAOTHD010000551         1629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_89322_length_1629_cov_3.970775, whole genome shotgun sequence.
ACCESSION   DAOTHD010000551 DAOTHD010000000
VERSION     DAOTHD010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89322_length_1629_cov_3.970775"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="P5219_09075"
     CDS             <1..180
                     /locus_tag="P5219_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26952.1"
                     /translation="AGEKLGLPVLYAGVHPDLLNEAAGILEGSSISVPLWPLSRYMML
                     PWEDGAMWSSGAAAE"
     gene            225..440
                     /locus_tag="P5219_09080"
     CDS             225..440
                     /locus_tag="P5219_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRX26953.1"
                     /translation="MAKGRIEVLEQYCKSCGLCVAACPVKVLRISEKTNAKGYRPVEQ
                     FKEGCIGCAMCATTCPDAAIEVFKIQE"
     gene            467..1528
                     /gene="vorB"
                     /locus_tag="P5219_09085"
     CDS             467..1528
                     /gene="vorB"
                     /locus_tag="P5219_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HRX26954.1"
                     /translation="MGRVLMKGTEAIAEAAIQAGCKYFFGYPITPQNEIPEYMSAHLP
                     EVGGVYVQAESEVASINMVLGGGATGYRVMTSSSSPGISLMSEGISYIAGCEIPAVIV
                     NVMRAGPGLGGILPGQADYLQATKGGGNGDYNLIVLAPSTLQECVDLTQLAFDLAQKY
                     RNPVMVAADGFMGQMMEAVEIKPSPTKDQPADPTWALGFMEERGGKRSHLHSLFLAPE
                     PLEEHNRKLQKKYGAMKAAEQRCECYHMEDAELLIAAYGTTARISRSAVNNLRAEGLK
                     VGMIRPVTLWPYPYDAFKTIPSTVKNILVAEMNAGQMVDDVKVATGCRYPVSFYGRTG
                     GFAPSVKEIEDECRRILAL"
BASE COUNT          340 a          481 c          511 g          297 t
ORIGIN      
        1 gccggggaga aactcgggct gcccgttctc tacgcggggg tacacccgga tcttttgaac
       61 gaagcggcag ggattcttga agggagttca atttccgtcc ccctctggcc cctttccagg
      121 tacatgatgc ttccctggga agacggggcg atgtggtcca gcggggcggc ggccgagtag
      181 gtgtttgttg catttcattt ctttctggaa ggggtgctaa ggcaatggcg aaaggacgca
      241 tcgaagttct cgagcagtat tgcaagagct gcggactgtg tgtggcggcc tgtcccgtga
      301 aggtcctccg gatttcggag aagaccaacg cgaagggcta tcggcccgtg gagcagttca
      361 aggaggggtg tatcgggtgc gccatgtgcg cgacaacgtg tcccgatgcc gctattgaag
      421 tgttcaaaat ccaggaatag tcctggtttt cggaggtgaa atcgacatgg gacgcgtatt
      481 gatgaaagga acggaagcca tagcggaagc cgccatacag gcaggctgca agtatttctt
      541 cgggtatccg atcactcccc agaacgagat tcccgaatac atgtccgccc atcttcccga
      601 ggtggggggc gtctatgtgc aggccgagag tgaagtggcc tccatcaaca tggtcctcgg
      661 cggcggcgcc acgggctacc gggtgatgac ctcctcgtcg agccccggca tctccctcat
      721 gtccgagggt atttcctaca tcgcaggatg cgagatcccc gcagtcatag tgaacgtcat
      781 gcgggccggc cccgggctcg gaggcatcct tcccggccag gcggactacc tccaggccac
      841 aaagggcggc ggaaacggcg actacaacct tatcgtcctc gcccccagca ccctccagga
      901 gtgcgtcgat cttacccagc tcgccttcga ccttgcccag aaataccgga accccgtcat
      961 ggtggctgcc gacggcttca tgggccagat gatggaagcc gtggagatca agcccagccc
     1021 cacaaaggac cagcccgccg atcccacatg ggccctcggt ttcatggagg agcggggcgg
     1081 caagcggagc cacctgcaca gcctcttcct tgctcccgaa cctcttgagg agcacaaccg
     1141 caagctgcag aagaagtacg gcgccatgaa ggccgccgag cagcggtgcg agtgctacca
     1201 catggaagac gcggagcttc tcatcgccgc ctacggaacc actgcgagaa tctcccgctc
     1261 ggcggtgaac aacctgcggg cggaaggcct caaggtcggc atgatccggc ccgttaccct
     1321 gtggccctat ccctacgacg ccttcaagac cattccttcc acggtgaaga acatcctcgt
     1381 ggcggaaatg aacgcagggc agatggtgga tgacgtgaag gtcgccaccg ggtgccggta
     1441 tccggtctcc ttctacggcc gcacgggcgg attcgccccc tccgtgaagg aaatcgagga
     1501 cgagtgcagg cgcattcttg ccctgtaaga gggagggaaa aatcatgagc gaagtgaaag
     1561 tgtacgaacg gccgaagagc tggatgccga acgtgagcag ccactactgc ccgggctgcg
     1621 gtcacggca
//
LOCUS       DAOTHD010000552         1629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_89357_length_1629_cov_2.507624, whole genome shotgun sequence.
ACCESSION   DAOTHD010000552 DAOTHD010000000
VERSION     DAOTHD010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89357_length_1629_cov_2.507624"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="P5219_09090"
     CDS             complement(<1..625)
                     /locus_tag="P5219_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRX26955.1"
                     /translation="MKAILAGALETGLGTVLSNVTVFVENGKIADIVEGLDPKNADEV
                     MDASEMVVTPGLIDAHTHIGTYCEGFPESMEDANDMVTPVVPHLRILDAIYQDDTAFA
                     DALAGGITCVQTLPGSGNVIGGQGAIIKIATASGGRKKTADEMVVKAPSSMKAALGEN
                     PIRVYKEKQKLPNTRMGNAALLRQALVEAENYRNKMIQARSKNEPAER"
     gene            843..1250
                     /gene="tsaA"
                     /locus_tag="P5219_09095"
     CDS             843..1250
                     /gene="tsaA"
                     /locus_tag="P5219_09095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HRX26956.1"
                     /translation="MECDDMRVEAIGKVHSPLKKREDCPRQGFEDAPEAEIEIFPAFL
                     EGLDGLEAGRELLVLTWLHLADRSVLQVHPRRNPEAPLKGVFATRSPARPNPIGLHRV
                     TLLEREGGRLRVAPLEALDGTPVADIKPVIDER"
     gene            complement(1260..>1629)
                     /locus_tag="P5219_09100"
     CDS             complement(1260..>1629)
                     /locus_tag="P5219_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRX26957.1"
                     /translation="DYDAVPACVFMNPEVGTVGLTEEEALARGGEIVSAKAYFAANGM
                     ALSLGESDGFVKVVARASDKTLLGVHIMGPEAASVLGEAALAVSRKLTAGDVARSVHA
                     HPTLCECFRDACLRVLEKEK"
BASE COUNT          270 a          461 c          544 g          354 t
ORIGIN      
        1 tccgttcagc gggctcgttc ttgctcctgg cctggatcat cttgttcctg tagttttccg
       61 cctccacgag agcctggcgg agcagggcgg cgttgcccat gcgggtgttg gggagcttct
      121 gcttctcctt gtacacccgg atggggttct ccccgagggc ggctttcatt gaggaggggg
      181 ccttcaccac catctcgtcc gccgttttct tccttcctcc cgatgccgtg gcgatcttga
      241 tgatggctcc ctggccgccg atgacgttgc cgcttcccgg gagggtctgg acacaggtga
      301 tgcctccggc gagggcgtcg gcgaaggcgg tgtcgtcctg gtagatggcg tcaaggatcc
      361 tgaggtgggg aaccacgggg gtcaccatgt cgttggcgtc ctccatgctc tcggggaatc
      421 cttcgcagta ggtgccgata tgggtgtggg catcgatgag cccgggggtg accaccattt
      481 ccgatgcgtc catgacctcg tccgcgtttt tcgggtcgag tccttccacg atatcggcga
      541 ttttgccgtt ttcaacgaaa acggtcacgt tggacagtac ggttccgagc cccgtttcga
      601 gggctcctgc gagtatggct ttcattcctg ggtgcctcct tgatggttgg ttgtggagtt
      661 tcgtttcttt caccggcatt gtacatcatg gcagagcctg ccggaatcgg tcttttgaat
      721 aaaaagaggg tgaagaattt gatgtatgct gatcaaaaaa tgtgctataa aatggtgcag
      781 cgccggcaga aacggatatg ccggagtatg ggcgtatcat gatgacggag gaggtctggg
      841 atgtggaatg cgatgacatg agggtggagg ccataggaaa ggttcattcg cccctgaaaa
      901 aacgggagga ctgcccgaga cagggattcg aggacgctcc cgaagcggag atcgagattt
      961 ttcctgcctt tctggaaggg ctggacggcc tggaggcggg gcgggagctg ctggtcctga
     1021 cctggctgca cctggctgac aggtcggttc ttcaggtgca tcccaggagg aatcccgaag
     1081 cgcccctgaa gggtgttttc gccacacgat cccccgcgag gccgaacccc atcggccttc
     1141 accgggtgac cctgctggag agggagggcg gccgtctccg ggtggctccc ctggaggccc
     1201 tggacggcac gcccgtggcg gacatcaagc cggtgatcga cgagcggtag acctgccgcc
     1261 tatttctcct tctccagaac gcgcaggcag gcatcccgga agcattcgca cagggtcggg
     1321 tgggcgtgga ccgagcgagc cacgtccccg gcggtaagtt tcctggagac ggcgagggcg
     1381 gcttccccga ggacggaggc tgcctccggg cccatgatgt ggactccgag gagggttttg
     1441 tccgaagccc tggccaccac cttcacgaag ccgtcgcttt ctccgaggga gagggccatg
     1501 ccgttggcgg cgaaataggc tttcgccgag acgatttccc cgccccgggc aagggcttcc
     1561 tcttctgtca gccccacggt ccccacctcg gggttcatga agacgcaggc cgggacggcg
     1621 tcgtagtcg
//
LOCUS       DAOTHD010000553         1628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_89442_length_1628_cov_2.375715, whole genome shotgun sequence.
ACCESSION   DAOTHD010000553 DAOTHD010000000
VERSION     DAOTHD010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89442_length_1628_cov_2.375715"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="P5219_09105"
     CDS             complement(<1..1257)
                     /locus_tag="P5219_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HRX26958.1"
                     /translation="MKKLVALVAVAVLVAFAAPALAANPFMDVPMNHWAYDAISQLSA
                     QGIVSGYPDGTFKGNAPMTRYEMASIVARALAVVDMNKASKQDVEMLKRLVVEFKDEL
                     DALGVKVDELDSRVAVLEENLGGWKFWGEFRFDAKFSDDDLYTSDEVDFTQSRYRLWY
                     SKVVDDKVTFIGRVTGSDLAYSRYYIDVKLPYDFTMWAGLWFYDWEDAKGLYNDNDAW
                     FGDQALEGFYFTRPFSMGNFSFYVAHADNKTLLEVVMPGDDDDDDDIVESYAGDYFRY
                     GLDVNFNPTENLRVGMGGTWFAFESLGTEGFDVTDFLDIDGSVYQADLTYNFTPDIAF
                     KGAYYMENLPGDVWDGPLDDSPSAWKAILDISQKAFGFTSVWIEYASFDPEFFLVDFA
                     GCDSDGNGPWDNYGAAITPGLGFTYSW"
BASE COUNT          384 a          435 c          429 g          380 t
ORIGIN      
        1 ccatgaatag gtgaaaccca ggccgggagt gatggccgca ccgtaattgt cccatgggcc
       61 gttgccgtcg ctgtcacagc cggcaaagtc taccaggaag aattccgggt cgaaggaggc
      121 atattctatc cagacgctgg tgaagccgaa agctttctgt gaaatatcaa ggatcgcttt
      181 ccatgcgctg gggctgtcat caaggggacc gtcccagaca tcgccgggca ggttctccat
      241 gtaatatgca cccttgaaag ctatatcggg agtgaaattg taggtcaggt ccgcctggta
      301 gaccgaaccg tcgatatcga ggaaatcagt aacgtcaaac ccttcggtgc caagggattc
      361 gaaggcgaac caggtgccgc ccattccgac cctgaggttt tcagtgggat tgaagtttac
      421 gtccaagcca tagcggaaat aatcgccggc gtaggattca acaatgtcat catcatcgtc
      481 atcgtctccg ggcatcacga cttccagaag ggtcttgttg tcagcatggg ccacatagaa
      541 ggagaagttg cccatggaga agggcctggt gaagtagaag ccttcgagag cctgatcgcc
      601 gaaccatgca tcgttgtcgt tgtagaggcc cttggcatct tcccagtcat agaaccacag
      661 accggcccac atggtgaagt cgtaaggaag cttcacatcg atgtagtaac ggctgtaggc
      721 aaggtcagaa ccggtcacac ggccgatgaa ggtgaccttg tcgtccacta ccttgctgta
      781 ccacagacgg taacggctct gggtgaagtc cacctcgtcg gaggtgtaaa ggtcgtcatc
      841 ggagaacttc gcatcaaaac ggaactctcc ccagaacttc catccgccaa ggttctcctc
      901 gagaaccgct acgcggctgt caagctcgtc taccttcact ccaagtgcgt caagctcgtc
      961 cttgaactcc actacgagtc tcttcagcat ctccacgtcc tgcttgctgg ccttgttcat
     1021 gtccaccacg gcaagagcgc gggctacgat ggaagccatc tcgtaacggg tcatgggtgc
     1081 attgcccttg aaagtgccat caggatatcc ggatacgatc ccctgggcgg aaagctggct
     1141 gatagcgtca taggcccagt ggttcatggg cacgtccatg aaaggattgg cggcaagtgc
     1201 cggtgcggca aatgcaacga gtactgctac tgctactaat gctacaagtt tcttcattgt
     1261 tgtctgcaac ccccttggtt gtttttttat ataacggcct tctcataacg gacagggggc
     1321 tctgcggaag tttcctcgtt cccttccgtt cctcccggta tccgttccta tgatcgggtc
     1381 cgccttcaac acccgctcta cccttttggc tgggggtcgt cggcacctcc tttcctgcct
     1441 tcgggtatga ccagcgacgt gttcaatgga aatgattgaa gcgcgaatta acaagatctc
     1501 atcggaggtg gagcatatta aattaacatt ataattgata aactgcaaaa tacaatatcg
     1561 ccttctccca aaaccctgtc gggatgacaa tgtgatcatg gtcataaagg tctgcctgga
     1621 aggaagga
//
LOCUS       DAOTHD010000554         1623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_89777_length_1623_cov_3.896046, whole genome shotgun sequence.
ACCESSION   DAOTHD010000554 DAOTHD010000000
VERSION     DAOTHD010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89777_length_1623_cov_3.896046"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            275..658
                     /locus_tag="P5219_09110"
     CDS             275..658
                     /locus_tag="P5219_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX26959.1"
                     /translation="MNEKNYSVLVCDDSAMIRKTLGGILGEWGIKNIFEAGNGNEAVD
                     MYREHHPDLVFMDIVMPEKTGLEALVEIRSEDPSARVIMASSTGTKKNLMTAIDAGAF
                     DFIQKPFEKDVIHTLLRKILKGELD"
     gene            658..1536
                     /locus_tag="P5219_09115"
     CDS             658..1536
                     /locus_tag="P5219_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26960.1"
                     /translation="MFSRHFGQFLLQEGIVTPQTLSRALARLSDARPLVGILALAKGF
                     MTPAQVREVNRIQKREDKRFGQIAIEKGYLTAEKLEELLSAQKNEHVLLGQILVEDRV
                     LSHEGLLRALELYREKSGLSPEGYEAMTGGDTDRAVASVLAGQPGGKNSLVRIWGELF
                     VRNVIRFVDPAAALDPLAEKGNGGLVTFLQPMKGDRELTVFISAEEPVLADLARRFSD
                     LASGMFDEMAREAVGEFLNVSNGLFTVNCSDKGIELDLLPQEEVPGNDPRLRRKPDGL
                     LPLLLPEGILWAGILC"
BASE COUNT          364 a          489 c          433 g          337 t
ORIGIN      
        1 cggaccaaat cccccaccgc cgacagatag tctttaaagt gctcatcaat atgcctgtgg
       61 acctgctcca ttctctcgtt cccctttccg gtacatgctg tttgggccta ttctattgta
      121 tcttccatga tgctgtccaa aacttccggc ctgttcggtg cgggaaggga aggcctaaat
      181 ttcccgaaaa aaacgatcat ttcctttgaa tggtcatgct ataattcaga caatatcctc
      241 tcccccggga gaattccatc agggaggttt tcccttgaac gaaaaaaact attccgtgct
      301 tgtctgcgac gactccgcca tgatccggaa aacactcggc ggcatactgg gcgaatgggg
      361 catcaaaaac attttcgaag ccgggaacgg caatgaggca gtggacatgt accgggagca
      421 ccaccccgat cttgtcttca tggatattgt catgcctgaa aaaaccggtc tcgaagccct
      481 ggtggaaatc cggagcgaag atccttccgc ccgggttatc atggcctcct ccacgggcac
      541 gaagaaaaat ctcatgaccg ccatagatgc cggagctttc gatttcatcc agaaaccttt
      601 cgagaaggat gtcatccaca ccctccttcg gaaaattctg aaaggagaac tggactgatg
      661 ttttcccggc atttcggtca gttcctcctc caggagggaa tcgtcactcc gcagactttg
      721 tcccgggcgc tcgccaggct gagcgatgcc cgccccctgg tgggaatcct cgccctggcc
      781 aaaggcttca tgaccccggc ccaggtcagg gaggtcaaca ggatccagaa gcgggaggac
      841 aagcggttcg gtcagatcgc catcgagaag ggctacctca ccgcggaaaa actcgaagaa
      901 cttctttcgg cccagaagaa cgaacacgtc ctcctgggac agattctcgt ggaggaccgg
      961 gtcctctccc acgaagggct gctcagggcc cttgaactgt acagggaaaa atcgggcctt
     1021 tctccggaag gctacgaggc aatgacaggg ggcgacaccg acagggccgt ggcatcggtc
     1081 ctcgcaggac agcccggcgg taagaattcc ctcgtcagga tctgggggga actcttcgtc
     1141 aggaacgtca tccgcttcgt ggaccccgcc gctgccctcg atcccctggc cgagaaagga
     1201 aacggcggac tcgtcacctt tctgcagccc atgaagggcg acagggaact gaccgtcttc
     1261 atctccgccg aggaacccgt cctggcggac ctggcccgga ggttctccga tctcgcgtcc
     1321 ggcatgttcg acgaaatggc ccgcgaggct gtcggggaat tcctcaacgt cagcaacggc
     1381 ctcttcacag tcaactgctc ggacaaaggg atcgaactcg atctccttcc ccaggaagag
     1441 gtgccgggaa acgatccccg cctccggaga aaacccgacg gcctcctgcc cctgcttctg
     1501 cccgaaggga tcctctgggc gggaattctc tgctgaaaaa cgaacaaacc tgcacggggg
     1561 cgctttctgc acatgcaggg agcgcccccg ttttttgatt tctgcgtttt ctcttgacaa
     1621 ata
//
LOCUS       DAOTHD010000555         1621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_90001_length_1621_cov_1.750958, whole genome shotgun sequence.
ACCESSION   DAOTHD010000555 DAOTHD010000000
VERSION     DAOTHD010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90001_length_1621_cov_1.750958"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1398)
                     /locus_tag="P5219_09120"
     CDS             complement(<1..1398)
                     /locus_tag="P5219_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRX26961.1"
                     /translation="MTKSPRLGDILVLSGAISDEQLQSALKEQKSSGLKLGELLLHKK
                     IITEGKLMEALATQLRLQIYSLSRYRPMPEALRMVPQGVAKRLQVVPLAVEDGNTLLV
                     AMADPLDVLAQDEIRMISGMELRIGIVSPTELERNLERLYSIQESLEGANLEFMDSSP
                     RDVDLTQAMGATTPDDAPVIQLVNQVIEQAVREGASDIHIEPFEKNTRVRYRIDGQLF
                     NAFDFPKHLHPAVSSRMKIMSGMDISEKRRPQDGRILIKVLGRRVDLRVSSLPTIYGE
                     KIVIRILDQGNAMVGLTKLGLDADDKMLVDQVCNAPYGIVLVTGPTGSGKSTTLYSVL
                     EQINKPEVNVVTVEDPVEYTIFGIGQVQVHERAGLTFNSTLRSILRQDPDKIMVGEIR
                     DGETAQLAIRAALTETGLAMPVAHLPTFVWLADVYDSVRQASVAEVVRALAVCHEFQI
                     PKAVLGENRSRNRTVI"
BASE COUNT          300 a          431 c          520 g          370 t
ORIGIN      
        1 tatgacggtt cggtttctgg agcggttctc acccaagacc gctttgggaa tctgaaattc
       61 gtggcagacg gccagcgcac ggacgacttc ggccaccgag gcttgccgga cgctgtcgta
      121 tacatccgcc agccagacga acgtggggag gtgcgccacc ggcatagcca atccggtttc
      181 ggtcagggcg gcccggatgg cgagctgggc cgtctctccg tcccggattt ctcccaccat
      241 tatcttgtcg gggtcctggc ggaggatgga ccggagggtg ctgttgaagg tgagtccggc
      301 ccgttcgtgc acctggacct ggccgatgcc gaagatggtg tattccacgg ggtcctccac
      361 ggtgaccacg ttgacttccg gcttgttgat ctgttcgagg accgagtaga gggtggtgga
      421 ttttccgctc cccgtggggc cggtgacgag gacgatgccg tagggtgcgt tgcagacctg
      481 gtccacaagc atcttgtcgt cggcgtcgag gcccagcttg gtgaggccca ccatggcgtt
      541 gccctggtcg aggatacgga tgactatttt ttctccgtag atggtgggca gggaggagac
      601 ccggaggtcc acccggcgcc cgaggacctt gatgaggatt cggccgtcct gggggcgccg
      661 tttttccgag atgtccatgc ctgacatgat tttcattcgg gaggagacgg cggggtgcag
      721 gtgcttcggg aagtcgaagg cgttgaagag ctgaccgtcg atgcggtagc gcacccgggt
      781 gttcttttcg aagggctcga tgtggatgtc cgaggcgcct tcccttaccg cctgttcgat
      841 gacctggttt acgagctgga tgaccggggc atcgtcgggg gttgttgcgc ccatggcctg
      901 cgtgaggtcg acgtcgcgcg gggaggaatc catgaattcg aggttggcgc cttcaagcga
      961 ttcctgtatg gagtacagac gctcaaggtt tcgctccagc tccgtgggcg agacgatgcc
     1021 gatgcgcagc tccatcccgg agatcatccg gatttcgtcc tgcgcaagaa catcaagggg
     1081 atccgccatg gcgaccagaa gcgtattccc gtcttcgacc gcgagaggaa caacttgaag
     1141 acgttttgcc acgccttgcg gtaccattcg taacgcttcg ggcattggcc ggtagcggga
     1201 aagcgagtat atttggagcc tgagctgcgt cgccaacgct tccatcaact tgccttcagt
     1261 gatgattttt ttatgaagca gcagctcccc aagcttcaaa ccggaacttt tctgctcctt
     1321 gagcgcggat tgcagctgct cgtcggagat tgctccggac agtacaagaa tatctcccag
     1381 tcgaggcgat ttggtcatgc tctctcccct ttatctcgat gacatgtttg atgctgcggg
     1441 attgtatggg acgataatag cgtaaaaggg gaaaatgtac aacgagaggg gaagaaccgc
     1501 tccggatttg tcagcgctta tagaagcaaa atgcgaatac gcaggaagcc gtcctgaggg
     1561 ttaactctgg acggcttcct gtgttctttc aaaaggattt ccggcggcga cctgctctcc
     1621 c
//
LOCUS       DAOTHD010000556         1610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_90825_length_1610_cov_3.090032, whole genome shotgun sequence.
ACCESSION   DAOTHD010000556 DAOTHD010000000
VERSION     DAOTHD010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90825_length_1610_cov_3.090032"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="P5219_09125"
     CDS             <1..365
                     /locus_tag="P5219_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26962.1"
                     /translation="PEIGLHMGRKFLPVSGWDMEFYGEWILSANDFSLESRWGIRWSP
                     WKNILAGVERAFPGNVTWYRLWIEGGVRAPYLWWRLSEDGDHHVGLGYRLNQRISLEI
                     HYDGRDEDKISIRALSDL"
     gene            393..1460
                     /gene="lpxD"
                     /locus_tag="P5219_09130"
     CDS             393..1460
                     /gene="lpxD"
                     /locus_tag="P5219_09130"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRX26963.1"
                     /translation="MNGERILLGDIARKLGGAVVGDSAAEVTRPASPTGARAGDICAV
                     WEVRDFEKIRVGVHVLASPDAFASRPELTGISVDDPRAAFPALLSLFQPVRKGRGVHP
                     SAVISPLAALSPDAWIGPLCVVEDGAVIGEGVRLRANVFVGEGCVLGAGTVVEPGAVL
                     LEKVRTGQNCLIHGGAVLGSDGFGILPAGEVSGPVKIPQLGGVVLGDEVEIGACTTVD
                     RGTLDDTVVGDFTKVDDHVHIAHNTVIGKNCIVVAMTGIAGSAVLEDNVIMAARSGVK
                     DHTRIGRGATVAANAGAIKDVPPGAVVSGFPARDHRENFRVQAAQQRLPELLLRVRKL
                     EQAVFPSDEKLPDRKQGGEGH"
BASE COUNT          295 a          457 c          550 g          308 t
ORIGIN      
        1 acccggagat cggtctccac atgggacgga agtttctgcc cgtttccggg tgggacatgg
       61 agttctacgg tgaatggatc ctgtccgcca acgatttttc cctcgagagc aggtggggca
      121 ttcgatggag cccctggaaa aacatcctgg ccggtgttga gcgggctttc cccggaaacg
      181 tcacctggta ccgtctctgg attgaagggg gcgtacgggc cccgtacctc tggtggcggc
      241 tcagcgagga cggagaccac cacgtggggc tcgggtaccg gctgaaccag cggatttccc
      301 tggaaattca ttacgacggc cgtgacgagg acaagatcag catcagggcg ctgagcgacc
      361 tctgagtgaa gccggaaaaa cggagaaaaa ccatgaacgg tgaaagaata ctactgggtg
      421 acattgcccg gaaacttgga ggagcggtcg tcggcgattc tgccgccgaa gtcacccgcc
      481 cggcttcgcc gacgggggct cgtgcggggg atatctgcgc ggtgtgggaa gtccgggatt
      541 ttgaaaaaat ccgggtcggt gtccatgtcc ttgcttcgcc tgatgctttt gcctcccggc
      601 cggaattgac cggcatttcg gtggacgatc cccgggccgc ctttcctgcc cttctttctc
      661 ttttccagcc cgttcgaaag ggcaggggag tccacccctc ggcggtgatt tctcccctcg
      721 cagccctatc ccccgacgca tggatcggtc cactctgcgt ggtggaagac ggtgccgtta
      781 tcggcgaagg agtccggctc cgggccaatg tcttcgtggg agagggatgt gtccttggcg
      841 cgggaacggt ggtcgaaccc ggcgcggtcc tcctcgaaaa ggtccgcacg ggacagaact
      901 gcctcatcca cggcggagcg gttctcgggt cagacggttt cggtatcctt cccgccggtg
      961 aggtgagcgg ccccgtcaaa atcccccagc tcggcggtgt ggttctcggt gacgaagtgg
     1021 agatcggggc ctgcacaacc gtggaccggg gaaccctgga cgatactgtt gtgggggatt
     1081 tcaccaaggt ggatgaccac gtccatatcg cccacaacac cgtcatcgga aagaactgca
     1141 tcgtggtggc catgacgggc atcgcaggaa gcgccgtcct tgaggacaat gtcatcatgg
     1201 ccgcaaggag cggtgtgaag gaccatacgc ggatcggcag gggagcaacc gtggccgcca
     1261 atgccggggc aatcaaggat gtgcctccgg gggccgtggt gtccgggttt cccgcccggg
     1321 accacaggga gaacttccgt gtccaggcag cccagcagcg ccttccggag ctcctcctcc
     1381 gggtccggaa gctcgagcag gcggtgttcc cttcggacga aaagctgccg gacaggaaac
     1441 agggagggga aggacactga tggaataccg ccggttgacc ggggagattg ttttttccgg
     1501 aaaggggctc cacacggggg aagactgccg cgtgatcctg tctccgggcg gtccggggga
     1561 gggtgtgacg gtggaccggg ggtgtggttt gtcccctctt gatgcctgca
//
LOCUS       DAOTHD010000557         1609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_90901_length_1609_cov_3.072716, whole genome shotgun sequence.
ACCESSION   DAOTHD010000557 DAOTHD010000000
VERSION     DAOTHD010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90901_length_1609_cov_3.072716"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="P5219_09135"
     CDS             <1..665
                     /locus_tag="P5219_09135"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRX26964.1"
                     /translation="EKAGGVRSDALAAAVEEQLLILLDEGFQDIILSAKSTSVHETVR
                     ANSLLALKYPGYPFHIGITESGCGRDGLVKSAAGLALLLGQGIGNTIRISLTESPLEE
                     VRAGYSLLRALDLRQRGGSLISCPTCGRKRIEVRTIVDLVTPFLESLPDGVTVAVMGC
                     EVNGPREAMDADFGIAGSPRGAVVFSKGTVLKEVPLEMVPELLEQMAEETRRQKGNEE
                     DN"
     gene            699..>1609
                     /gene="argF"
                     /locus_tag="P5219_09140"
     CDS             699..>1609
                     /gene="argF"
                     /locus_tag="P5219_09140"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRX26965.1"
                     /translation="MPVNLKGRSFLTLMDFSKDEIYYLLDLAADLKAKKRAGIRGNAL
                     QGKNIALLFEKASTRTRCAFTVACIDEGAHPEFLGKNDIHLGGKEDVKDTARVLGRMF
                     DGIEYRGFKQSMVEELAEYAGVPVWNGLTDVDHPTQILADFLTLQENFGRILKGVRLV
                     YCGDGRNNMSNALMIGCAKLGMHYVVASPASLFPDAGLLEQCRALAAVSGGSVTVFED
                     PFEAVKGADAVYTDVWVSMGEEEKKKERESLLRPFQVSMNLMKSSGKDGTIFLHCLPA
                     VKGQEVTEDVFEAPFSKVFDEAENRMHT"
BASE COUNT          372 a          404 c          505 g          328 t
ORIGIN      
        1 tggagaaggc aggaggagtc cgttccgacg cccttgcggc agcggtggaa gaacagctcc
       61 tgattctcct ggatgaaggc tttcaggata taatattgtc cgccaagtcc acatccgtac
      121 atgaaacggt ccgggcgaac tctcttctcg ccctgaaata tcccggctat ccgtttcaca
      181 tagggatcac ggaatcggga tgtggacggg acggtttggt gaaaagcgct gcggggttgg
      241 ccctgcttct cggtcagggt atagggaata ccatccgcat cagccttacg gaatctcctc
      301 tcgaggaggt ccgggcggga tattccctgc tcagggccct ggacctgaga cagaggggcg
      361 gaagccttat ttcatgcccg acctgcgggc ggaaaaggat tgaggtcagg acgatcgtcg
      421 acctggtgac gcccttcctt gagagtctgc cggacggtgt gaccgtggcc gtgatgggct
      481 gtgaagtaaa cggacctcgg gaagccatgg acgccgattt cgggattgcc ggatctcccc
      541 ggggtgcggt ggtcttttcg aaggggaccg ttttgaagga ggttcccctg gagatggtac
      601 cggaacttct ggaacagatg gccgaggaaa cccgacggca aaaaggaaat gaagaggaca
      661 attaataaac tgggtaaatg gaaaggggaa agagcacaat gccggtgaat ctgaaaggaa
      721 gaagctttct tacgcttatg gatttctcga aggatgaaat ctattacctg cttgaccttg
      781 ctgcggacct gaaggcgaaa aagagggcgg ggatacgggg caatgcactc cagggaaaga
      841 atatcgcgct gctgtttgaa aaagcgtcca cccgaacccg ctgcgctttt actgtcgcct
      901 gcatcgacga gggggcccac ccggaattcc tcggcaaaaa cgacatccac ctcggtggca
      961 aggaagatgt aaaggacacc gccagggtcc tggggaggat gtttgacggc atcgaatacc
     1021 ggggcttcaa gcagtccatg gtggaagaac ttgcggaata cgccggagtt cccgtctgga
     1081 acggactgac cgacgtggac cacccgaccc agattctcgc cgacttcctc acccttcagg
     1141 agaatttcgg caggattctg aagggagtgc gtctcgtgta ctgcggcgac ggaagaaaca
     1201 acatgtccaa cgctctgatg atcgggtgcg ccaaactggg aatgcattac gtggtggcct
     1261 ccccggcgtc tctcttcccg gatgcgggcc ttctggaaca gtgcagggcc cttgccgcgg
     1321 tttccggcgg ctcggtcacg gtttttgaag accccttcga ggccgtgaag ggagccgatg
     1381 cggtgtacac cgacgtatgg gtttccatgg gcgaagagga aaagaagaag gagcgggaat
     1441 cccttctgag gcccttccag gtttccatga atctcatgaa gtcttcggga aaggacggaa
     1501 ccatattcct tcactgcctt cctgcggtta aaggacagga ggtcacggaa gacgtgttcg
     1561 aagctccttt ctcgaaggta ttcgatgagg cggagaacag gatgcacac
//
LOCUS       DAOTHD010000558         1607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_91040_length_1607_cov_3.353737, whole genome shotgun sequence.
ACCESSION   DAOTHD010000558 DAOTHD010000000
VERSION     DAOTHD010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91040_length_1607_cov_3.353737"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /gene="rplL"
                     /locus_tag="P5219_09145"
     CDS             complement(<1..221)
                     /gene="rplL"
                     /locus_tag="P5219_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870240.1"
                     /note="present in two forms; L12 is normal, while L7 is
                     aminoacylated at the N-terminal serine; the only multicopy
                     ribosomal protein; 4:1 ratio of L7/L12 per ribosome; two
                     L12 dimers bind L10; critically important for translation
                     efficiency and fidelity; stimulates GTPase activity of
                     translation factors; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRX26966.1"
                     /translation="MTRDEMIQAIETMTVLELSELVKALEDKFGVSASAPAMAMPMMA
                     MAGAPAAAAEEEKTEFDVVYKAPGANKINV"
     gene            complement(285..818)
                     /gene="rplJ"
                     /locus_tag="P5219_09150"
     CDS             complement(285..818)
                     /gene="rplJ"
                     /locus_tag="P5219_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRX26967.1"
                     /translation="MPAQVKFEQVEELRERLSKTQAVFVAEYRGMTVAQITALRAQVR
                     AAGGEMKVAKNTLMKIAMEQEGLTAFPDDMAYGPNVFALAYGDPVAVAKALRDFSKEK
                     TNKAFLLKGGALGHSILGVDQVSALADLPSKDVLIGQVVRTIAAPISGFLSVLNGPIR
                     GLATCLNQIKEKKEQAA"
     misc_feature    complement(841..984)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(1007..>1607)
                     /gene="rplA"
                     /locus_tag="P5219_09155"
     CDS             complement(1007..>1607)
                     /gene="rplA"
                     /locus_tag="P5219_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRX26968.1"
                     /translation="FDESIEVHFRLGVDPRHADQQVRSTVVLPHGTGITKRVLVLAHG
                     EKMKEAEEAGADFVGGEDLVTKISGGWLDFDAVIATPDMMKSVGRLGKLLGPRGLMPS
                     AKTGTVTFELADAIREIKAGRVEFRVDKFGIIHNGVGKKSFTEEQLYDNVRTIFQAIL
                     KARPSSVKGTYVKSFSVASTMGPGIKIDPSAAQKEVAGE"
BASE COUNT          322 a          468 c          433 g          384 t
ORIGIN      
        1 acgttgatct tgttggcacc gggggccttg tagaccacgt caaactcggt cttctcttct
       61 tcggcggcgg cggcgggagc accggccatg gccatcatgg gcatagccat cgcaggagcg
      121 gaagcggaca cgccaaactt gtcctcaagt gctttcacga gctcagaaag ctcgagaacg
      181 gtcatcgttt caatagcctg gatcatttca tcacgggtca ttctttttat cctccttgga
      241 tatgtatcag caatttgtgt gcgccgcagc gcggcctcac gctgctatgc agcctgttcc
      301 ttcttttcct tgatctggtt gaggcaggtc gccagtcccc ggatcggccc gttgagcacc
      361 gaaaggaatc cggagatcgg cgctgcgatg gtacggacaa cctgtccgat aaggacgtcc
      421 ttgctgggaa gatcggcaag ggcgctgacc tggtcgactc ccagaatgga atgtcccagg
      481 gcgcctccct tcaaaaggaa agccttgttg gtcttttcct tggaaaaatc acggagtgcc
      541 ttggccaccg ctacggggtc accgtaggca agggcgaaaa cgttgggacc gtatgccata
      601 tcatcgggga aagctgtaag cccttcctgt tccatggcga tcttcatgag ggtgttcttg
      661 gccaccttca tttccccgcc agcggcgcga acctgggccc gcagggcggt gatctgtgcg
      721 acggtcattc cgcggtattc ggccacaaag accgcctggg tctttgacag cctttcgcga
      781 agttcttcaa cctgttcaaa ttttacttgt gccggcatgt actcacctcc ttgtctcaca
      841 gcattaaaca gaaaatcccg ggcacaccgg gcccgggata aaaaacagct ttacagccgc
      901 atttcagccg gaacctcggc gggcggaacg tatccattga cccttttggg ggcccgctgt
      961 ctgcagttca cgcagtattt cgtttgtcaa atgccgtatt tcctgcttat tctccggcaa
     1021 cttccttctg ggcagccgag ggatcgatct tgattccggg gcccatggtg gaagctacag
     1081 aaaagctctt tacataggtt cccttgacag aggaagggcg ggccttcaga atcgcctgga
     1141 atattgtcct gacgttatca taaagctgct cttccgtaaa gctcttctta ccgacaccgt
     1201 tgtggatgat tccgaacttg tcgaccctga actctaccct gcctgccttg atctccctta
     1261 tggcgtccgc gagctcgaaa gtcacggttc cggtctttgc gctgggcatg agtcccctgg
     1321 ggccaagaag ctttccgagg cggcccaccg acttcatcat gtcgggggtg gcgatgacag
     1381 catcaaaatc aagccagccg ccggagattt tcgtgacgag atcttctcct ccgacaaaat
     1441 cagcgccggc ttcttcggct tctttcatct tctcgccgtg ggccaaaacg agcactctct
     1501 tggtaatgcc cgtaccgtgg ggaagaacaa cggtactcct gacctgctgg tccgcatgcc
     1561 ggggatcgac cccgagccga aaatggacct cgatggactc gtcaaac
//
LOCUS       DAOTHD010000559         1607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_91056_length_1607_cov_2.757732, whole genome shotgun sequence.
ACCESSION   DAOTHD010000559 DAOTHD010000000
VERSION     DAOTHD010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91056_length_1607_cov_2.757732"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="P5219_09160"
     CDS             <1..392
                     /locus_tag="P5219_09160"
                     /inference="COORDINATES: protein motif:HMM:NF024208.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRX26969.1"
                     /translation="CIGCFSCMYACSRMVRNAGGSGKAALRVKGYAGVEGAFSIRVCA
                     RCEDPDCAKACPTGALTPAPNGGVRLKKDLCISCGACVKACAISALQWDEEERQPIPC
                     IFCGQCVKYCPNDVLAMTEKPEGEDRR"
     gene            389..>1607
                     /locus_tag="P5219_09165"
     CDS             389..>1607
                     /locus_tag="P5219_09165"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRX26970.1"
                     /translation="MTARTLHVDLGSGTGRILDDGPAFSEWLGGSAAATELLFRHGDP
                     KGDPLAPESPVIFAIGPFSALFPVATKTAALFKSPLTGELGESHAGGRLAMSLREAGI
                     DALVITGRADRPSYLAIENDTVAVKSASTFWGQSATATDRILREAESKTGRKISVVRI
                     GPAGERLSPMACATVDGSRHFGRLGLGAVLGSKNLKAIVVSGTKTLPLANPKEFKTVY
                     DVLYDRVVRSKEMKKYHDLGTAANVKPLSMIRGLPTRNFSQGNFEGADAISGERFAEK
                     FLVQHTACAHCQCGCIHLAELREEFAPYHYSTNRVSYDYELIYAMGSMLSLSSPEDIM
                     KLLLATEKQGWDVISLGCTLAWATESFLRGNLSLEETGGLSLSFGDGAAYLEVLERTA
                     KGQGEFYRDLEKGC"
BASE COUNT          324 a          529 c          487 g          267 t
ORIGIN      
        1 agtgcatcgg ctgcttcagc tgcatgtacg cctgctcccg gatggtccgg aatgccggag
       61 gcagcggcaa ggctgccctc agggtcaagg gctacgccgg tgtcgagggg gccttctcca
      121 tcagggtctg cgcccgctgc gaggatccag actgtgcaaa ggcctgcccg acgggggccc
      181 tgacccccgc cccaaacggc ggcgtacggc tcaaaaaaga cctctgcatc tcctgcggcg
      241 cctgcgtaaa ggcctgtgcc atcagcgccc tccagtggga cgaggaggag aggcagccca
      301 ttccgtgcat cttctgcggc cagtgtgtga aatactgccc caacgacgtc ctcgccatga
      361 ccgaaaaacc ggaaggagag gacaggagat gacggcaaga accctgcacg tcgacctggg
      421 ctccgggaca ggccggatac tcgatgacgg accggcgttc tcagaatggc tcggcggatc
      481 ggcggccgcc acggaactcc ttttccgcca cggcgacccg aagggagatc ccttggcccc
      541 ggaaagccct gtgatcttcg ccattggccc cttcagcgcc ctctttccag tcgcgacaaa
      601 aacagcggct ctcttcaaat cccctctcac aggggagctc ggggaatccc acgccggagg
      661 acggctggcc atgtctctcc gggaggcggg catcgacgcc ctcgttatca ccggcagggc
      721 ggaccgcccc tcctacctgg ccatcgaaaa cgacaccgtc gccgtcaaat cggcctcgac
      781 cttttggggg cagtcggcca cggccaccga ccggattctg cgggaggcgg agagcaaaac
      841 gggacggaag atttccgtgg tccgcatcgg ccccgcaggg gagcggctct cccccatggc
      901 ctgcgccacg gtggacggtt cccgccattt cggccgcctg ggactcggag ccgtcctggg
      961 gagcaaaaac ctcaaggcca tcgtcgtcag cgggacgaaa accctgcccc tggcaaatcc
     1021 gaaggaattc aagacagtct atgacgttct ttacgaccgg gtcgtccggt cgaaggaaat
     1081 gaaaaagtac cacgacctgg gcaccgcggc caacgtgaag cccctctcca tgatacgggg
     1141 cctccccacc cggaacttct cccagggcaa cttcgagggg gccgacgcca tctccgggga
     1201 acggtttgcc gaaaaattcc tggtgcagca cacggcctgc gcccactgcc agtgcggctg
     1261 catccacctc gccgaactgc gggaggaatt cgctccctac cattacagca cgaacagggt
     1321 ctcctacgac tacgagctga tctacgccat gggaagcatg ctcagccttt cctcacccga
     1381 ggatataatg aagcttctcc tggccacgga aaaacaggga tgggacgtca tttccctcgg
     1441 gtgcaccctt gcgtgggcca cggaatcctt cctccgggga aatctttctc tcgaagaaac
     1501 gggagggctc tctctttcct tcggagacgg tgcggcctac ctcgaggtgc tcgaacgcac
     1561 ggccaaagga cagggagaat tctacaggga cctggagaag gggtgtg
//
LOCUS       DAOTHD010000560         1606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_91130_length_1606_cov_2.916183, whole genome shotgun sequence.
ACCESSION   DAOTHD010000560 DAOTHD010000000
VERSION     DAOTHD010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91130_length_1606_cov_2.916183"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="P5219_09170"
     CDS             complement(<1..329)
                     /locus_tag="P5219_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_083777221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(84..86),aa:Sec)
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRX26971.1"
                     /translation="MAEEKVSCAVPASTVEGSSNEPAPAAEEKKEAVRMGVTVGMKAP
                     DFTAPAYYKGKFTTVKLSDFAGKWVSLCFYPGDFTFVUATEVSAVADRYAEFQALGVE
                     VISVSVD"
     assembly_gap    485..494
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            554..>1606
                     /locus_tag="P5219_09175"
     CDS             554..>1606
                     /locus_tag="P5219_09175"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRX26972.1"
                     /translation="MGIATDLIIVVLAGLFGGLAARRLNQPLILGYILAGIVVGPYTG
                     GITLSDVDQVESLAEIGVALLLFSLGLEFSLRGILPIRAVALGGTGIQIALTVGLGWG
                     IGQFMGWETVPSLWFSVAIVSSSTAVILKTLASRGQLGTLSSRVMLGMSIVQDILVIP
                     LMVLLMNLDSTGFSVSGILMPVGKVVLFVAAMFYIGARYIPLVLKAVAKWESRELFLL
                     TVTAIGLGIGYLTYLFELSFAFGAFMAGLVLSESDYGRRALSDLIPVRDIFGLLFFVT
                     IGMLLDPFFLASRFGTVLGLFAAAVFGKGLILAAVTRLFGYGRVIPLAVFFGMIPVSE
                     IAFIVLQHGLESGALDQ"
BASE COUNT          296 a          465 c          433 g          402 t
ORIGIN      
        1 ctgtcaacgc tcacggatat cacttcaacc ccgagagcct ggaattccgc ataacggtcg
       61 gcgaccgccg acacttcggt agctcagacg aaggtgaaat caccgggata gaagcagaga
      121 gaaacccact ttccggcgaa atcggagagt ttgacggtgg tgaacttgcc cttgtaatat
      181 gccggagcgg taaaatccgg cgccttcatg ccgacagtga cacccatgcg aacagcctcc
      241 ttcttttcct ccgcggcggg cgcgggttca ttactcgatc cttcaacggt ggacgcggga
      301 acggcgcatg atactttttc ctcagccatg aaacctcctc ctttcaagat accctgaaat
      361 atacgacaca tagctggaat tatttatatc agtatactat aacaagaacg ttacgagcca
      421 tctttttcat ttttattata tggtaatcat tttaaatgtc aaggattttt gtgtcttttt
      481 tcacnnnnnn nnnnttttgc actttccgcc ggaacatgta aaaataggcc atcgtcagga
      541 aagggatgag tttatgggca ttgcaacaga cctcatcatc gtcgtcctcg ccggcctttt
      601 cggcggtctt gccgccagga ggctgaacca gccgctcatc ctcggctata tcctcgccgg
      661 aatcgtggtc ggaccctata ccgggggaat caccctgagc gatgtggacc aggttgagag
      721 cctggccgag atcggtgtgg cccttcttct tttctccctc ggcctcgagt tttccctcag
      781 gggcattcta cccatcaggg cggtggccct gggcgggacg ggaatccaga tagccctcac
      841 agttggcctc ggctggggca tagggcagtt catgggatgg gagaccgttc cgtccctgtg
      901 gttttctgtg gccatcgtct cctcaagcac tgccgtcatt ctgaaaaccc tcgcatccag
      961 ggggcagctc ggtacccttt caagcagggt gatgctgggc atgtccatcg tccaggacat
     1021 cctcgtgatc cccctcatgg tgcttctgat gaacctcgac tccacgggct tctcggtttc
     1081 gggaatcctg atgccggtgg gaaaggtcgt gcttttcgtg gccgccatgt tctacatcgg
     1141 ggcccggtat atccccctgg tgctgaaagc ggtggcgaaa tgggagtccc gggagctttt
     1201 cctgctcaca gtcaccgcca tcggactcgg catcggctac ctgacctacc tgttcgagct
     1261 ttccttcgcc ttcggggcct tcatggcggg actcgtgctg agcgaatcgg actacggcag
     1321 aagggctctc agcgacctta tccccgtgag ggacattttc ggcctcctct ttttcgtcac
     1381 catcggcatg ctcctcgatc ccttcttcct cgcttcaagg ttcggcacgg tgctcggcct
     1441 gtttgccgcg gccgtcttcg ggaaggggct gatccttgcg gcggtcacca ggcttttcgg
     1501 ctacgggagg gtcattcccc tggcggtgtt cttcggcatg attcccgtgt cagaaatcgc
     1561 cttcatcgtg ctccagcacg gtctggagtc gggagctctg gaccag
//
LOCUS       DAOTHD010000561         1605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_91240_length_1605_cov_2.476774, whole genome shotgun sequence.
ACCESSION   DAOTHD010000561 DAOTHD010000000
VERSION     DAOTHD010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91240_length_1605_cov_2.476774"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="P5219_09180"
     CDS             <1..827
                     /locus_tag="P5219_09180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRX26973.1"
                     /translation="HRLEGDILWGRGTVDAKGPLCAFAAAGGRVSLPPDWAITLVAAT
                     GEEADSRGALHRIPRHRPAACIIGEPSGTDGVTIGYRGNLRAVLAASDGGAHRSGNAG
                     PITGVLRCAADILDLVDSLDDPALPVIRRPSGAVVSMEGKEQGERSGTAELDIRLPEG
                     SSPGEWMEFLRGSAARRGVSISFRGVTPAHVEDKNNPVARALRLAIRKNGNTPRVLAK
                     GGTADFNLASAWKCPMAAYGPGDSTLDHTSEERISLADFSASVAVLDGALPLILAG"
     gene            complement(881..>1605)
                     /locus_tag="P5219_09185"
     CDS             complement(881..>1605)
                     /locus_tag="P5219_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HRX26974.1"
                     /translation="PTPGMCKGHVQGNLVILPKDLAYDFLVFAQRNPKPCPILDVTEP
                     GNPEPKIVAPGADLSTDLPRYRVWVNGECVEEPTDVKKYWRDDLVGFLLGCSFSFEGA
                     LLEAGIPVRHIEQNRNVPMYITNIQCAPAGALKGPMVVSMRPIPAAQVPKAVLCTGRF
                     PAVHGSPVHIGDPSLIGIADVNKPDMGDPVDINPGEVPVFWACGCTPQAAIMDVKPPF
                     CITHAPGHMFIADPKDADYAVF"
BASE COUNT          265 a          519 c          562 g          259 t
ORIGIN      
        1 aacaccgcct tgaaggggac attctctggg ggcggggaac ggtggacgcc aaggggcccc
       61 tgtgcgcctt cgccgccgcg gggggacggg tttccctccc cccggactgg gccatcaccc
      121 tcgtggccgc caccggagag gaggccgact cccggggcgc cctccaccgg attccccggc
      181 atcgtcccgc cgcctgcatc atcggggaac cctccggaac ggacggggtg actatcggct
      241 accggggcaa cctccgggcg gtgctcgccg catccgacgg aggagcccac aggagcggta
      301 acgcggggcc catcacgggg gtcctccggt gtgccgccga catcctggac ctggtggaca
      361 gcctggatga ccctgcgctg ccggtcatcc ggcgtccctc aggcgccgtg gtctccatgg
      421 agggaaagga acagggggaa cggtccggaa cggcggagct ggacatccgc cttcccgagg
      481 gcagcagccc cggggagtgg atggagtttc tccgggggag cgccgcacgg cggggcgtgt
      541 ccatttcctt ccggggcgtg acacccgccc acgtggagga caagaacaac cccgtggccc
      601 gggccctcag gctggccatc cggaaaaacg ggaacactcc ccgggttctt gccaaaggcg
      661 gcacggcgga cttcaacctc gcgtcggcgt ggaagtgccc catggcagcc tacgggcccg
      721 gcgacagcac actggaccat acgtccgaag aacggatctc ccttgccgat ttctctgcct
      781 ccgtggcggt cctcgacggt gcccttcccc tcatactggc aggataaaaa aaccggaggg
      841 gcattcgctc cctccggttt cgttgtgccg tacatgggga tcagaagacg gcgtagtcgg
      901 cgtccttcgg atcggcgatg aacatgtgcc ccggcgcgtg ggtgatgcag aaggggggct
      961 tcacatccat gatggcggcc tggggcgtgc agccgcaggc ccagaagacg ggcacctctc
     1021 ccgggttgat gtccaccggg tcgcccatgt cgggcttgtt cacgtcggcg atgccgatga
     1081 gagacgggtc gccgatgtgg acgggagacc cgtggaccgc cgggaagcgt cccgtgcaca
     1141 ggaccgcctt gggaacctgg gcggcgggga tggggcgcat gctcaccacc atggggccct
     1201 tcagggctcc ggcgggagcg cactggatat tggttatgta catggggacg ttccggttct
     1261 gctcgatgtg gcgcaccgga atgcccgcct cgagcagggc cccctcgaag gagaaggagc
     1321 accccaggag gaagcccaca agatcgtccc tccagtattt cttcacgtcc gtgggttcct
     1381 cgacgcactc gccgttcacc cacacccggt accggggaag gtccgtggag agatcggccc
     1441 ccggggccac aatcttaggc tcgggatttc ccggctcggt gacgtccagg atgggacagg
     1501 gtttcgggtt cctctgggcg aagacgagga aatcgtaggc caggtctttg gggaggatca
     1561 ccaggttccc ctggacgtgt cccttgcaca tgcccggtgt gggga
//
LOCUS       DAOTHD010000562         1604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_91297_length_1604_cov_2.894771, whole genome shotgun sequence.
ACCESSION   DAOTHD010000562 DAOTHD010000000
VERSION     DAOTHD010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91297_length_1604_cov_2.894771"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="P5219_09190"
     CDS             complement(<1..705)
                     /locus_tag="P5219_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRX26975.1"
                     /translation="MNHPYQDIPICLPDSFSWDASRRIPVEECGEPLVSAGYLPEKIL
                     VSPQYYVHGIEGAIPECFVRRSVLLRLSRAADMLPRGCRFVLFDSWRSRVVQTSLFQK
                     FRSELRERMPLLDDQEITTLASQFVALPSISPTHPSPHVTGGAVDLTIVDETGLCLPM
                     GTEFDETTEKSATDFFEKKLAAKERLTSSDEESLVNRRLLYAVMIRAGFTNYPDEWWH
                     FDYGNQNWALLHGEKAA"
     gene            complement(722..>1604)
                     /locus_tag="P5219_09195"
     CDS             complement(722..>1604)
                     /locus_tag="P5219_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRX26976.1"
                     /translation="AGLTAGSALIGPIIPPSIFMIIYATMTNTSIGGLFAAGILPGLL
                     LGGAFMAMNYVYSHRYQVPVADFGEVRKRKKEILTRSIVALFAPVIIVGGIVTGIVTP
                     TESGALAVAYCVLAGFFYTGQLGLRVLAESFYETVRLSSSIFLIMGAATVIGWLLKWE
                     QVPQRFATLILQAGLLDKPWLLMIVLSAIIFPVGMFMEEVSTLALLTPIFAPIALKAG
                     IDPFHFGVVMTLNVTIALITPPMGACNYIVAAVGRVKLADVFVHIWPFIAVALLVLLV
                     IISFPPLTTLVPRLLNL"
BASE COUNT          374 a          432 c          486 g          312 t
ORIGIN      
        1 tgccgccttt tctccatgaa gcagggccca gttctggttt ccgtagtcga aatgccacca
       61 ttcgtcggga taattcgtaa aaccggccct gatcatgacg gcataaagca gtctcctgtt
      121 gacgagggac tcctcgtctg aagaggtcag ccgctccttc gccgccagtt tcttctcgaa
      181 aaaatccgtg gccgattttt ccgtggtctc gtcaaattcc gtccccatag gaaggcagag
      241 tcccgtttca tccactatgg tcagatccac cgctccccct gtgacatgag gggacgggtg
      301 ggtcggcgat atggagggca aggctacgaa ctgggatgcg agcgtggtga tttcctggtc
      361 gtcgagcagg ggcatccttt ccctcagttc ggacctgaat ttctggaaga gggacgtctg
      421 gaccaccctg gacctccagg aatcgaacag gacaaagcgg cacccccggg gcagcatatc
      481 cgccgccctg ctgaggcgaa gaaggactga acggcggacg aaacactcgg gaatcgcccc
      541 ctcgatgccg tgaacataat actgggggct cacaagaatc ttttccggca gatatcctgc
      601 ggaaacgagg ggttcaccgc attcttccac ggggatacgc cgggaggcgt cccaggaaaa
      661 ggaatccggc agacagatag gtatatcctg atagggatgg ttcattttcc tgctccttcc
      721 atcagaggtt cagcagccgc ggaacgagcg tggtcagcgg cgggaacgaa atgatgacca
      781 gcagcacaag aagggcgacg gcaatgaagg gccagatgtg aacgaatacg tcggccagct
      841 tcactcttcc cactgccgca acgatgtaat tgcaggctcc catgggaggc gttataaggg
      901 ctatggtgac gttgagggtc atgacgacgc cgaaatgaaa ggggtcgatc ccggccttca
      961 gggcaatggg ggcaaaaatc ggcgtgagaa gggccagggt ggaaacttct tccatgaaca
     1021 tgccgacggg aaagatgatc gcgctcagca cgatcatgag aagccatggc ttgtcaagca
     1081 gccccgcctg gagaatgagg gttgcgaacc tctggggtac ctgctcccac ttcagcagcc
     1141 atcctatgac ggtggcggcg cccataatga ggaaaattga actggaaagg cgaaccgttt
     1201 catagaagga ctcggcaaga accctgagac cgagctgccc ggtgtagaaa aaaccggcca
     1261 gaacgcagta ggcgactgca agagctcccg actccgtggg ggtgacgatt cccgtgacta
     1321 ttcccccgac gatgatgacc ggggcgaaaa gagccactat ggaacgggtc agaatctctt
     1381 tcttccgttt ccgcacttct ccgaagtcgg ccacgggaac ctggtacctg tgggaataga
     1441 cgtagttcat ggccatgaag gctccgccga ggagcaggcc gggcaatatt ccggcggcga
     1501 acagccctcc gatggaggta tttgtcatgg ttgcgtatat gatcatgaag atgctcgggg
     1561 gaatgatggg cccgattagg gcgcttcctg ccgtgagccc tgcc
//
LOCUS       DAOTHD010000563         1602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_91470_length_1602_cov_2.965740, whole genome shotgun sequence.
ACCESSION   DAOTHD010000563 DAOTHD010000000
VERSION     DAOTHD010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91470_length_1602_cov_2.965740"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="P5219_09200"
     CDS             <1..615
                     /locus_tag="P5219_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRX26977.1"
                     /translation="WVCSPNNPTGTLPLKEELLRLLSACDGTGTLLALDEAYVNFAPP
                     GSSALPLMRPGLLLLRSMTKDYGLTGLRLGYIMGDPRLVGLLASLQPPWSVNACAQAA
                     GLAALRARGYYEAQWAGLRSLASGLARDLAEAGFPPLPSAGNFLLFRAGDSERLQAFL
                     WKRRILVRDCSSFGLPGYVRVGVKSGDENRRLVECLAAYRKEAS"
     gene            615..1160
                     /gene="cobU"
                     /locus_tag="P5219_09205"
     CDS             615..1160
                     /gene="cobU"
                     /locus_tag="P5219_09205"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF004469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HRX26978.1"
                     /translation="MGRLTLVLGGSRSGKSEFAENLVREAECAGKKVVYIATCESRPE
                     DREMAERIDRHRKRRPSSWRTEEEALALEETLLGLESGTVALVDCLGLWVSNLLFSLP
                     EQQEGKEEEILRRAEAFCRAASASAADVVAVSSETGMGVVPPAPLGRIYRDLLGLANQ
                     AAARSAGEAWLVVAGLPLKLK"
     gene            1229..>1602
                     /locus_tag="P5219_09210"
     CDS             1229..>1602
                     /locus_tag="P5219_09210"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HRX26979.1"
                     /translation="MTTLEQAIARIRPLCAPSMEEARRRQDSLTKPRGSLGKLEDLSV
                     QLAGIQRTSSPAIRHKVILTMAGDHGVVDEGVALYPREVTVQQVLNFLGGGGGVNVLA
                     RHVGARVVLVDMGVAHDFEPREG"
BASE COUNT          270 a          501 c          544 g          287 t
ORIGIN      
        1 tgggtgtgtt ccccaaacaa ccccacggga acccttcccc tgaaagagga gcttctccgc
       61 ctgctctccg cctgcgacgg gacgggcacc ctgctcgccc tcgacgaggc ctacgtgaac
      121 ttcgcccccc cgggatcgtc ggcattgccg cttatgaggc ccggccttct tcttctccgg
      181 tccatgacca aggactacgg cctgacgggg cttcgtctgg gctatatcat gggagatccc
      241 cggcttgtgg gtctcctcgc ctccctgcag cctccctgga gcgtgaacgc ctgtgcccag
      301 gccgccggtc ttgcggccct gcgggcccgg ggatactatg aagcccagtg ggccggtctc
      361 cggagccttg cctccggact ggcccgggat cttgcggagg ccggtttccc tcctcttccg
      421 tccgcgggga attttctgct tttccgggcc ggggattccg aaaggctcca ggcgtttctc
      481 tggaaacggc gcattctggt gagggactgt tcctccttcg gtcttcccgg atatgtccgg
      541 gtgggcgtga agagcgggga cgagaacagg cggctcgtgg agtgcctggc tgcgtaccgg
      601 aaggaggcgt cctgatgggc aggctgaccc ttgtcctcgg aggaagcagg agcggcaaga
      661 gcgaattcgc cgagaatctg gtccgggagg ccgagtgcgc ggggaaaaag gtggtctaca
      721 ttgccacctg cgaatccagg cccgaagacc gggagatggc ggagcggatc gaccggcaca
      781 ggaagcgtcg gccgtcatcg tggagaaccg aggaggaggc cctggccctc gaagagaccc
      841 ttctggggct tgaaagcggt accgtggccc tggtggactg cctcggcctc tgggtctcga
      901 accttctctt ctccctgccg gaacagcagg aagggaaaga ggaggagatt ctcaggagag
      961 cggaggcgtt ctgccgggcc gcctccgcct ccgccgccga cgtggtggcc gtctccagcg
     1021 aaacaggcat gggggtggtt ccccccgccc cgctcggaag gatctaccgg gatctccttg
     1081 gactggccaa ccaggcggcc gcacgctctg caggggaagc ctggctcgtg gtggccggac
     1141 tgcctctgaa actaaaatag agaatacgtt ccaggcggtc cacggcggcc gttcatccaa
     1201 cccttcatcg ggaaaaccag gaggcatcat gacgacacta gaacaggcaa tcgcccggat
     1261 ccgcccgctc tgcgcacctt ccatggaaga ggcgagacgg cggcaggact ctctcaccaa
     1321 gccgaggggc agcctcggta agctcgagga tctttccgtg cagctcgcag gaatccagcg
     1381 cacctccagc cccgctatcc ggcacaaggt gatcctcacc atggcggggg atcacggggt
     1441 ggtggacgag ggagtggccc tttatccccg ggaggtcacg gtgcagcagg tgctcaactt
     1501 tctgggcggc ggcgggggag tgaacgttct tgcccgtcac gtgggagcca gggttgtcct
     1561 ggtggacatg ggggtggccc atgacttcga gccccgggaa gg
//
LOCUS       DAOTHD010000564         1601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_91584_length_1601_cov_2.078266, whole genome shotgun sequence.
ACCESSION   DAOTHD010000564 DAOTHD010000000
VERSION     DAOTHD010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91584_length_1601_cov_2.078266"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="P5219_09215"
     CDS             complement(<1..948)
                     /locus_tag="P5219_09215"
                     /inference="COORDINATES: protein motif:HMM:NF019824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HRX26980.1"
                     /translation="MKALFGLEERILDTKRDMEAREALLASRNLAVPGGESLVLGFFR
                     DDRLVATGALVGNILQGIAVAGEIEGEGAAAAVTSALLKKAVERGMRKVFLYSKPQEA
                     RIFRELGFSLLAEVVVERSGLGAALMEWGAEGIEDWKAGMAKFAEGKPRGAGAAVVNC
                     NPFTLGHRKLLEHAAERSPWLYVLVVEEDRSLFPFDVRLRLVREGISDLENVTVLPGG
                     PYVISSATFPSYFTRVPAGGDSRKITELYAALDLEMFRRHVAPALGVGIRFVGTEPYC
                     PVTSVYNDLMKRILPAGGEGIPPVEVREIPRFERDGSPVS"
     gene            1152..>1601
                     /locus_tag="P5219_09220"
     CDS             1152..>1601
                     /locus_tag="P5219_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRX26981.1"
                     /translation="MPLLKNVPDLRTKPMRDIIYEHLRKAIVRGELKADSTFTDGEIA
                     EEFGVSRTPVREAVQKLEADGYIERVPMKGNRVCGISPYELAHSMAIRKALETLAVRY
                     AALRITADELLRLEELLAQADSVFTQYIGDELLERFFPIVKSFNHTVF"
BASE COUNT          346 a          532 c          424 g          299 t
ORIGIN      
        1 gctcacggga gaaccgtccc tctcgaaccg gggaatctcc cggacctcca cgggcgggat
       61 cccctcccct ccggccggaa gaatccgttt catcaggtca ttataaacgg aggttacggg
      121 gcagtagggt tccgttccca cgaaccggat ccctacgccg agggcgggag ccacgtgccg
      181 gcggaacatc tccaggtcga gagcggcata gagttcggtg atcttccggg aatcgccccc
      241 agcgggaacc ctggtgaaat aggacgggaa tgtggcggac gaaatgacgt agggacctcc
      301 cggcagcacc gtcacgttct ccaggtccga aatcccctcg cggacgaggc gcaggcggac
      361 gtcgaagggg aaaagtgacc ggtcctcctc aactaccagc acgtacagcc acggactcct
      421 ttccgcggca tgctcaagga gtttcctgtg gcccagggtg aaggggttgc agttcaccac
      481 agccgcacct gctccacggg gttttccttc ggcgaatttc gccattccgg ccttccagtc
      541 ttcgataccc tcggcacccc attccataag ggcagctccc aggccggacc gttcaaccac
      601 cacctccgcg aggagagaaa aacccagttc ccggaatatt cgggcctcct ggggtttcga
      661 gtagagaaag accttcctca ttccacgctc aacggccttt ttcagcaggg ccgacgtcac
      721 agcggcggcg gctccttcgc cctcaatttc accggcgacg gcgatgccct ggagaatgtt
      781 ccccaccagc gcacccgtgg ctaccagccg gtcatcccgg aaaaaaccga ggaccaggct
      841 ctctcctccc ggcaccgcaa ggttccttga agcaagcagt gcctcccgcg cctccatgtc
      901 cctcttcgta tcgagaatgc gctcctccag cccgaacagg gccttcaccc cctttccgga
      961 cagctttcgc cggcggcccg gagcgaattt cccgccgaac cggcctgcgc atgtcctgaa
     1021 aatatacaaa aaaatgggaa tcaaaccact ttacactttg cctttccggg gatattatac
     1081 gacatggatg ctggatgttg gatccaactt ttatcggaaa aaataaaatt tttatttccg
     1141 gaggaacgac catgcctttg ctgaagaacg ttccggatct ccgcaccaag cccatgagag
     1201 acatcatcta cgaacacctc cgcaaggcca tcgtccgggg cgaactcaag gccgactcca
     1261 ccttcaccga cggcgaaatc gccgaagagt tcggcgtgag ccgcacacct gtcagggaag
     1321 ccgtacagaa gctcgaggcc gacggctaca tcgagcgggt tcccatgaaa gggaacaggg
     1381 tctgcggcat ctccccctac gagctggccc actccatggc catccggaag gccctggaaa
     1441 ccctggctgt ccgctacgcc gccctccgga tcaccgccga tgaactgctc cggctggaag
     1501 aactcctggc ccaggctgac agcgtcttta cccaatacat cggcgatgag cttctcgaac
     1561 gttttttccc catagtgaag agtttcaacc ataccgtctt c
//
LOCUS       DAOTHD010000565         1598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_91771_length_1598_cov_3.344783, whole genome shotgun sequence.
ACCESSION   DAOTHD010000565 DAOTHD010000000
VERSION     DAOTHD010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91771_length_1598_cov_3.344783"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1598
                     /gene="dnaE"
                     /locus_tag="P5219_09225"
     CDS             <1..>1598
                     /gene="dnaE"
                     /locus_tag="P5219_09225"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRX26982.1"
                     /translation="VDPEGHTTRDKRNRNHHLILLAENDEGYHNLVKLTSIANTDGFY
                     GKPRIDHSLLARYKSGLIASSACLAGEIPSLILEGREKEALDRAVLYRDIMGKENFFL
                     EIMYNAIPEQAVVNRAIVRMAREHGFPLIATNDAHYLSKDDYDWHEILLCVQTKSSLE
                     DENRMSFSSNDFYFRSPEEMDMLFGAELPDALDNTVAIAERCNVRLPLGERDYKLPNL
                     KLPEGETLESNLEKEARQGLAERMKGNVPEEYAQRLEYELGVINSMGFAGYFLIVAGI
                     IGAAKKRGIPIGPGRGSAAGSVVAWSLKITELDPIRYNLLFERFLNPERISMPDIDTD
                     VSDKGRDELLHYISEHYGSDRVSQIVTFGRMKSRAAVTDVGRVLGMAVRDVNAITRLI
                     PPMGVHSIGEAVEQVPELADLKKNNPMVARVLDIASNIEGLARHCSQHAAGVVITPMP
                     VTDLVPVRRIGDGQIVTQYPMEPIEKLGLVKMDFLGLRTLSVIEEALQNISLSGKPVP
                     DMEHIPLDDEASYEMLQKADTLGV"
BASE COUNT          379 a          465 c          454 g          300 t
ORIGIN      
        1 cgtcgatccc gaagggcata ccaccaggga caaaagaaac aggaaccatc atctcatcct
       61 ccttgccgag aacgacgagg ggtaccacaa cctggtgaag ctcacgtcca tcgccaatac
      121 cgacggattc tacggaaaac cgaggataga ccattctctc ctcgcaaggt acaagtcggg
      181 gctcatcgcc tcttccgcat gtcttgcggg tgagattccc tctctcatcc tcgagggcag
      241 ggagaaggaa gctctcgacc gggcggtact ctacagggac attatgggga aagagaattt
      301 cttcctcgag atcatgtaca acgccattcc cgagcaagcc gtcgtgaaca gggctatcgt
      361 ccgcatggca agggaacacg gctttcccct catagcaaca aacgacgctc attatctctc
      421 gaaagacgat tacgactggc atgaaatcct cctctgcgtc cagacgaaga gcagtcttga
      481 ggacgagaac aggatgtcct tctcaagcaa cgatttctac ttccgctctc cggaggaaat
      541 ggacatgctt ttcggagcgg agctgcccga cgcgctggat aacaccgtgg ccatcgccga
      601 acggtgcaat gtccgtcttc cgcttggaga gagggattac aagctcccga atctcaagct
      661 tcccgaaggg gaaacgctgg agagcaatct tgaaaaggag gcccggcagg gccttgcgga
      721 gcgcatgaaa ggcaatgtcc cggaagaata cgcacaacgg ctggaatatg agctcggcgt
      781 tataaactcc atggggtttg ccggatactt cctcatcgtt gccggaatca tcggagccgc
      841 gaaaaagcgg ggaataccta taggaccggg cagaggctcg gcggcggggt cagtcgtggc
      901 gtggagtctc aagatcaccg agctggaccc catacggtac aatcttctct tcgaacggtt
      961 cctgaacccc gagcggatca gcatgcccga tatcgacacc gacgtatcgg acaaggggcg
     1021 ggatgaactg ctccactaca tctcggaaca ctatggctcc gacagggttt cccagattgt
     1081 caccttcggc cggatgaaaa gcagggccgc agtgacggac gtgggaaggg tcctgggaat
     1141 ggctgtccgg gatgtgaacg ccatcacccg gctcattccc cccatggggg tccattccat
     1201 cggcgaggcg gtggaacagg ttcccgaact cgccgatttg aaaaagaaca atcccatggt
     1261 ggccagggtt ctcgacattg cctccaacat cgagggcctt gcccggcact gctcccagca
     1321 cgccgccgga gtggtcatca cccccatgcc ggtgacggat ctcgttcctg tgaggaggat
     1381 cggcgacggg cagattgtta cccagtaccc catggagccc atcgagaagc tcggtcttgt
     1441 aaagatggac ttcctcggcc tcaggacgct ttccgtcatc gaggaggcgc tgcagaacat
     1501 ctcgctcagc ggaaaaccgg tcccagacat ggagcatatc cccctggacg atgaggcctc
     1561 ctacgaaatg ctccagaagg cggacaccct cggcgtgt
//
LOCUS       DAOTHD010000566         1597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_91864_length_1597_cov_2.972763, whole genome shotgun sequence.
ACCESSION   DAOTHD010000566 DAOTHD010000000
VERSION     DAOTHD010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91864_length_1597_cov_2.972763"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="P5219_09230"
     CDS             complement(<1..418)
                     /locus_tag="P5219_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26983.1"
                     /translation="MKNVVNGMKGYFDRGMEFLFRLVDECPDDLWTKKGGGFFYWQQV
                     YHAFYCVDYFLLPPDGEMADEPYGRAVAMFSEVPPVPPSKETVREFGAVMKKKADEWI
                     ASLDDGALGLRHEGMTARKKAETCNGAVFASLCGHNM"
     gene            653..>1597
                     /locus_tag="P5219_09235"
     CDS             653..>1597
                     /locus_tag="P5219_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRX26984.1"
                     /translation="MKMRTACALILAVLCLATAVPGMAAARPVTLVDFSWDSVQVHNR
                     IAAYIIEKGYGRETDFIFAESLPGFMGMERGDADFSMEGWVDNLLEYWEKTTKSGKMV
                     SLGTNFPDAPQGWYVPTYMIKGDEKRGIKPVAPDLKSVADLPKYWELFKDPENPKKGR
                     LLNGPAGWKVTSINEMKIRGYGLDKQYDAFSAGSETALSAAIKRAYDRGEPVLAYYWE
                     PTWVMGLLDMTKLEEPPYDEKLWNDENLYACAIPSVRVLVVANTKFVEANPDIAAFLR
                     NYTTTLEQNNKVLGFMFNEKADTQTAAVWFLKNYEDVWK"
BASE COUNT          381 a          459 c          412 g          345 t
ORIGIN      
        1 acatgttgtg gccgcacagg gaggcaaaga ccgccccgtt gcaggtttca gcctttttcc
       61 tggcggtcat tccctcgtgc ctgagtccga gggccccgtc atcaagggag gcgatccact
      121 cgtcggcctt ctttttcatg accgcgccga actctctcac ggtttccttc gaaggaggta
      181 ccggaggcac ttcgctgaac atggcaacgg ccctcccgta gggctcgtcc gccatttccc
      241 cgtccggcgg gagaaggaag taatccacgc agtaaaaggc atggtacacc tgctgccagt
      301 aaaaaaaccc gccgcctttt tttgtccaga gatcgtccgg acattcgtcg accagtctga
      361 aaagaaattc catccccctg tcaaaatacc ccttcatgcc gttcacgaca tttttcattg
      421 aaacgccccc tttgacttgc agattaacta aatagtatat ccccatggcc cctgttcttc
      481 cactggaacg gcttaaaata tacattacag aattgtctat cttgacaaat agtgcgcctt
      541 gagttttaat cagataacat aaatttcctg ccagggaaac agtcaactta tttctgcgga
      601 tgtaatcctc gcacaacatc caagattcta tcttattcca ggaggtgttt gcatgaaaat
      661 gcgaaccgca tgtgcgctta ttcttgccgt cctgtgcctg gccacggccg tgcccggcat
      721 ggctgccgcc cgcccggtga cgctggtcga cttcagctgg gacagcgtcc aggtccataa
      781 ccgcatcgcc gcctacatca tcgaaaaagg atacggcaga gaaacagatt tcatctttgc
      841 ggaatccctt ccgggtttca tgggcatgga gcgcggcgac gccgattttt ccatggaggg
      901 ttgggtcgac aaccttctcg agtactggga gaagacgaca aaaagcggga agatggtgag
      961 cctcgggacg aatttcccgg acgctcccca ggggtggtac gttcccacct acatgatcaa
     1021 gggggatgaa aagcggggga tcaaaccggt cgccccggat ctcaagtccg tcgcggatct
     1081 tccgaagtac tgggaactct tcaaagaccc cgaaaacccc aaaaaaggac gccttctcaa
     1141 cggtccggcc gggtggaagg tgacctccat caacgagatg aaaatcaggg gctatgggct
     1201 ggacaagcaa tacgacgcct tttccgcggg ctcggaaaca gccctttcgg ctgccataaa
     1261 gagagcctat gaccgcggcg aaccggttct ggcctattac tgggaaccca cgtgggtcat
     1321 gggtcttctg gacatgacca agctcgagga gcccccctac gacgagaagc tctggaacga
     1381 cgaaaacctg tacgcatgcg ccattccttc cgttcgcgtc ctcgtggtcg ccaatacgaa
     1441 gttcgtggag gccaatcccg acatcgcggc atttttgagg aactacacaa ccaccctcga
     1501 gcagaacaac aaggtcctcg ggttcatgtt caacgaaaag gccgatacgc agacggcggc
     1561 cgtctggttc ctgaaaaact acgaagatgt gtggaag
//
LOCUS       DAOTHD010000567         1595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_92023_length_1595_cov_3.634416, whole genome shotgun sequence.
ACCESSION   DAOTHD010000567 DAOTHD010000000
VERSION     DAOTHD010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92023_length_1595_cov_3.634416"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="P5219_09240"
     CDS             complement(<1..361)
                     /locus_tag="P5219_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRX26985.1"
                     /translation="MAGIIERNAWFSAMGVSHEFPDVLRTSGPDNPAVTAVTSRDQTA
                     PLVGDNGLFRVYPLFPSDGSVPFELFSVEMERGAFSFSDSHDPGTIEYIHVFEGWITV
                     KTGQEEYYLSPGDSLRHR"
     gene            598..1557
                     /locus_tag="P5219_09245"
     CDS             598..1557
                     /locus_tag="P5219_09245"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HRX26986.1"
                     /translation="MNDILQRIGELGIVPVVKLEKAEDAVSLGRALLEGDLPIAEVTF
                     RTDAAEESIRTLSKELPELLVGAGTVLTKEQVRRAVDAGAKFIVTPGFNPSVVDYCVE
                     NHIPITPGINSPSQIEMALERGLNVVKFFPAEQSGGLPMLKAMSAPYGSVKFIPTGGV
                     DTKNMLSYLAFNKILAVGGSWMVKPEMVAAGQFDEIARITREAVYTMHGFELAHLGVN
                     CSDGQEAADGAEKIASLFGMAVKDGNSSIFAGKAFEFMKSPYLGEKGHVAISCNDVAR
                     ALAFLKSKGIGSLPETEKKKDGRTAAIYLDLHVGGFIFHLLQK"
BASE COUNT          347 a          458 c          482 g          308 t
ORIGIN      
        1 cccggtggcg gagagagtcc cccggggaga gatagtattc ttcctgcccc gttttcactg
       61 tgatccaccc ttcgaagaca tggatgtact ctatcgtgcc ggggtcgtgg gagtcggaaa
      121 aggagaaggc tccccgctcc atttccacgg aaaacagctc gaagggaacg gatccgtcgg
      181 agggaaaaag agggtagacc cgaaaaaggc cgttatcccc taccaggggg gctgtttgat
      241 ccctggaggt gaccgccgtc acggcaggat tgtccggacc ggaggttcgg aggacatccg
      301 gaaattcatg ggacactccc atggccgaaa accaggcgtt ccgttctatt atgccggcca
      361 ttgcttctca cccccggaga ggacgggtca tgaacgctct tccctgcagt cctccaaagc
      421 cgaagtcttg cctgttcggt tctttcggaa acactctatc actccgggga ataacggtca
      481 acggaggctt tttacccgaa acatcgtttt aatcagtgaa aagccctctt gacggaagga
      541 agccctattg tctattcttg gtgcggattt ccattacgga aaggagacag gcatttcatg
      601 aacgacatac tgcagcgcat cggcgagctg ggcattgtcc cggtcgtgaa gctcgaaaag
      661 gcggaggacg ccgtttccct gggcagggct ctcctggagg gagacctccc cattgcggag
      721 gtgaccttcc gcaccgacgc cgccgaagag tccatcagga ctctttcgaa ggagcttccg
      781 gagctcctgg tgggcgcggg aacggtcctg acgaaggaac aggtcaggcg ggcggtggac
      841 gcgggagcga aattcatcgt cacccccggg ttcaatccct ccgttgtgga ctactgcgtg
      901 gagaaccata tccccatcac cccggggatc aacagccctt cccagatcga aatggccctc
      961 gagcgggggc tcaatgtggt gaaattcttt cccgccgaac agtccggcgg acttcccatg
     1021 ctcaaggcca tgagcgctcc ctacggttcg gtgaagttca tccccaccgg gggcgtcgac
     1081 acgaagaata tgctttccta ccttgccttc aacaagatcc tcgcggtggg cggaagctgg
     1141 atggtgaagc cggaaatggt ggcggcgggc cagttcgacg agatcgcccg gattacccgg
     1201 gaggcggtct ataccatgca cggcttcgag ctcgcccatc tcggagtgaa ctgttctgac
     1261 gggcaggaag cggcggacgg cgccgagaag atcgcttccc tcttcggcat ggcagtcaag
     1321 gacggcaaca gctccatctt cgccggaaag gccttcgagt tcatgaagtc accctacctc
     1381 ggcgagaagg ggcatgtggc catatcctgc aacgacgtgg cccgagctct cgccttcctg
     1441 aaatccaaag gcatcggctc ccttcccgag acggagaaga agaaggacgg aagaacagcg
     1501 gcaatctacc tggacctgca cgtgggcggg ttcatcttcc acctgctgca gaagtagatt
     1561 cctgaggaaa cgaaacgggg gaggcccttc aaggg
//
LOCUS       DAOTHD010000568         1593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_92217_length_1593_cov_2.733420, whole genome shotgun sequence.
ACCESSION   DAOTHD010000568 DAOTHD010000000
VERSION     DAOTHD010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92217_length_1593_cov_2.733420"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="P5219_09250"
     CDS             complement(<1..1145)
                     /locus_tag="P5219_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26987.1"
                     /translation="MEMKLMAPAVFLLSFFLSSVLPAAGESDPFFAKIWEKALPKLEK
                     SLEVFDRRKDLPDSSFFGEDKQSNTEKYEEMLDEVFAILIGSELQNARQEFNFLEMKI
                     EQAGDEITVLLKERIGAPEETWNPLKKTRKKIDEKIADLREDIEGLTARKTDLKRSMR
                     EALAKMGVKVEERQLDVLLSSVSGDDVMHLLLAADSIKKINQQILSLLQGGQGSVQSA
                     RKYTGVHMVLVDLYLHANLDVLERIGTEYIPRLEDIMKTARRLGEEARQMLAKAGRDE
                     KNRQVLEANVKANERTVHTAELYRQYLRGQENALRGASEKLKRDYFVARNTYDTVKTG
                     SDLISLIQVSMSTLEGVFGFVVPDLALVYDQELMKEFERITERIRTG"
     gene            1285..>1593
                     /locus_tag="P5219_09255"
     CDS             1285..>1593
                     /locus_tag="P5219_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase beta chain"
                     /protein_id="HRX26988.1"
                     /translation="MKKVSILNHVIGPVMRGPSSSHTAGPWRIGRMAANLLEAPPDRA
                     VFTFHPSSSLAVCFRDQGSDLAFAAGLLRRPLTDPDFPRILSLAPGHGLDIRFEKAPF
                     P"
BASE COUNT          333 a          482 c          367 g          411 t
ORIGIN      
        1 ttccctgtcc ggatgcgctc agtaattctt tcaaattcct tcatgagttc ctgatcgtac
       61 acaagagcaa gatcggggac cacaaatccg aagaccccct caagggtact catggaaacc
      121 tggataaggg aaatgagatc actgcctgtt tttacagtat cgtaggtgtt ccgcgcaacg
      181 aaataatctc ttttcagttt ttccgaggcc cccctgaggg cgttttcctg tcccctcagg
      241 tactgccgat agagctccgc cgtatgcacg gtacgttcgt tcgctttgac gttggcttcc
      301 aggacctgcc tgtttttttc gtcccgcccg gccttcgcca gcatctgtct cgcttcttct
      361 cccagtctgc gggcagtttt cattatatct tcaaggcggg gaatatactc tgtcccgatt
      421 ctttccagca cgtcgaggtt ggcatgaagg tagagatcca ccagcaccat gtggaccccc
      481 gtatatttcc tagccgactg gacgcttccc tggcctccct ggagaagact caggatctgc
      541 tggttgatct tcttaatgct gtctgcggcc aaaagaaggt gcatcacgtc gtcaccgctg
      601 acgctggaga ggagcacatc aagctgccgc tcttcaactt tgactcccat tttcgccagg
      661 gcctcacgca tggatctttt caggtccgtt tttcttgctg tcaggccttc aatgtcttcc
      721 ctgagatcgg cgatcttttc atcgattttt ttcctggttt tcttcagggg attccacgtc
      781 tcttcaggcg cacctatcct ctccttcagg agcaccgtga tttcatcccc cgcctgttct
      841 atcttcattt cgagaaaatt gaactcctgg cgggcgttct gaagctccga cccgataagg
      901 atcgcaaaga cctcgtcaag catctcctcg tacttttcag tgttcgactg cttatcctca
      961 ccgaagaagc ttgaatccgg aagatctttc ctgcggtcaa acacttcgag ggatttttcc
     1021 agcttgggga gagccttctc ccagattttc gcaaaaaagg gatcgctctc ccccgccgcc
     1081 ggaaggacgg aagaaaggaa aaaactcaaa agaaaaactg ccggggccat caatttcatt
     1141 tccatcacct cataacagta atctatcata aatgcctccc aaaaggaaaa gaaaattact
     1201 gatacaccgc cttacagacc cttaaaaatg ggggtgatat actttctgaa gatcatattc
     1261 tcctccccgg aggtgacggc ggcaatgaaa aaggtgagca ttctgaatca cgttatcggg
     1321 ccggtgatga gaggtccctc gagctcccac accgcaggcc cgtggagaat cggccgcatg
     1381 gcggcaaacc tgctcgaagc gcccccggac cgggccgttt tcacattcca tccctccagt
     1441 tccctggccg tctgtttccg tgaccagggc agcgacctgg cctttgcggc gggactcctc
     1501 cgccggcccc tcacggatcc cgattttccc cggatcctct ccctggcccc cggccacggc
     1561 cttgacatcc gcttcgagaa ggcacctttt ccc
//
LOCUS       DAOTHD010000569         1592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_92298_length_1592_cov_2.557580, whole genome shotgun sequence.
ACCESSION   DAOTHD010000569 DAOTHD010000000
VERSION     DAOTHD010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92298_length_1592_cov_2.557580"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..715
                     /locus_tag="P5219_09260"
     CDS             221..715
                     /locus_tag="P5219_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26989.1"
                     /translation="MSEPVHALDLSHEEWIDVSTKRWGLPKYRADQLCQWIYDKKVFN
                     VHEMTNLSKELRDKLVYDLMILPPIPVTEETSKDGTRKFLWQLYDGERIESVLLTHGN
                     HTTACISSQVGCPLNCSFCATGQSGFRRDLTAGEITGQLLAMEKRLGTNIGNVVFMGM
                     GEPS"
     gene            763..1254
                     /locus_tag="P5219_09265"
     CDS             763..1254
                     /locus_tag="P5219_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26990.1"
                     /translation="MRNLGARHITISTSGIVPGILDLAEFPLPVRLSVSLHAPNDALR
                     SKIMPVNRKYPLGPLVDALRTYQQKTGERITLEYVMIQKVNDEPELAFEMAALLSGLG
                     VYVNLIPYNPVKDVYQRSTPERIKAFAAVLTRLGIENEIRREKGTDINAACGQLRRRE
                     SEN"
     gene            1251..1541
                     /locus_tag="P5219_09270"
     CDS             1251..1541
                     /locus_tag="P5219_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRX26991.1"
                     /translation="MNPPRHPGGPAVALRDLPNGAFGRIAEMPAKDRCAERLEALGLR
                     VGKVVEKVSGMPFHGPVTLQLDGRQIALGWKISSSVLVVPLSPAGGAAMGHE"
BASE COUNT          361 a          484 c          441 g          306 t
ORIGIN      
        1 ctgggccgag gaattcgccg ggtttattct tgacttccac gaggaacaac atatcctttc
       61 ctgagtatcc tccgttcttc ccgggagagc gccggtaccg ttccacgccg tccgggggca
      121 taggccgccc ttttcccatt gacacatatg ccggcttcat gacatcatat attgttattt
      181 caaggggaat tgtccccgaa atgccaccag gaggcacaca atgagcgaac cggtacacgc
      241 cctcgatctt tcccatgaag aatggatcga tgtcagcaca aaaaggtggg gcctcccgaa
      301 gtatcgggcg gaccagctat gccagtggat ttacgacaaa aaagtcttca acgtccacga
      361 aatgaccaac ctctcgaagg aactgaggga caagcttgtt tatgacctga tgatcctccc
      421 tcccatcccc gtcacggagg aaacctcgaa agacggcacc aggaaatttc tctggcagct
      481 ctacgacggg gagagaatcg aatcagtgct tctcacccac gggaaccata ccacggcctg
      541 tatttcaagc caggtggggt gcccgctgaa ctgctccttc tgcgccacag ggcagtccgg
      601 cttccgccgg gatctcacgg caggggagat cacgggacag cttctcgcca tggagaaacg
      661 cctcgggacc aatatcggca atgtggtctt catgggaatg ggagagccct cctgaacatc
      721 cgggcggtct tcaaggccat tgccatcctg aattcgccga aaatgagaaa tctgggtgcc
      781 cggcatatca ccatctccac ctccggcatc gtgccgggga ttctcgacct ggcggaattt
      841 cccctgccgg tccgtctttc ggtctccctc catgcgccca acgacgcgct ccggagcaag
      901 atcatgcccg tgaacaggaa atatcccctg gggcccctgg tggacgccct caggacgtac
      961 cagcagaaga ccggggaaag gatcaccctc gagtacgtca tgatccagaa ggtgaacgac
     1021 gaaccggaac tggcctttga aatggcggcc ctcctgtcgg gcctcggcgt gtacgtgaac
     1081 cttatcccct acaatccagt gaaggacgtg taccagcggt ccacccccga aaggatcaag
     1141 gccttcgccg cggttctcac caggctcgga atcgaaaacg aaatccgccg ggaaaaggga
     1201 acggatatca atgctgcatg cggccagctt cgaaggaggg aatcggagaa ttgaaccctc
     1261 cccgtcatcc gggaggtccc gcagttgccc tgagggatct ccccaacggc gcctttggcc
     1321 gcattgcgga gatgccggca aaggaccgct gcgccgaacg tctcgaagcc ctgggtctcc
     1381 gggtcggcaa agtggtggaa aaagtctccg gaatgccctt tcacggtcct gtgaccctcc
     1441 agctcgacgg gagacagatc gccctcggct ggaaaatatc ctcttcggtc ctcgtcgttc
     1501 ccctttctcc cgccggagga gctgccatgg gccatgaatg aggcagcagg gaaaaaactg
     1561 cttctggtgg gcaacccaaa cgtggggaaa ag
//
LOCUS       DAOTHD010000570         1591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_92399_length_1591_cov_2.543620, whole genome shotgun sequence.
ACCESSION   DAOTHD010000570 DAOTHD010000000
VERSION     DAOTHD010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92399_length_1591_cov_2.543620"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /gene="eno"
                     /locus_tag="P5219_09275"
     CDS             complement(<1..170)
                     /gene="eno"
                     /locus_tag="P5219_09275"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522203.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRX26992.1"
                     /translation="MSSIIGVHGREIIDSRGNPTVEVEVWLDSGASGRAAVPSGASTG
                     TYEAVELRDGGPR"
     gene            complement(242..520)
                     /locus_tag="P5219_09280"
     CDS             complement(242..520)
                     /locus_tag="P5219_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HRX26993.1"
                     /translation="MRLDKYLKLARLVKRRTVAQEMVTLGAVRINGRQCKPAAEIRTG
                     DVLDIAYPSRILTVEILCDDENVLKRGAPGGAYGVREERRADPAEKPW"
     gene            complement(598..>1591)
                     /locus_tag="P5219_09285"
     CDS             complement(598..>1591)
                     /locus_tag="P5219_09285"
                     /inference="COORDINATES: protein motif:HMM:NF024755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRX26994.1"
                     /translation="LILVEGYMDAIRLHLGGFEETAASLGTALTEEQAALIRRLADRC
                     CICYDSDLAGQEAAMRGMYVLQKAGLDVRVVELPRGKDPDDLLSLPEGRELFEKALAS
                     ARPLILHHVFLRGEMLRSPDKRRKAADEILSGLGSLSPVDVAPYLPRVAASMGIFPHE
                     LSALLEEYRKRGGHREHSAADTERPVAPRREDEGDEVEAAVCALLWKDDHIRRTADEI
                     QVLSLVEDERIQTVAAALLSAPSREDVEARWHSLGETFPLRAIARGGDWCEGLGDARA
                     AWDVVLDILGKRKARREYERIREKLSRGEADREELLRFREVARLLKSSPAEKAK"
BASE COUNT          258 a          541 c          446 g          346 t
ORIGIN      
        1 cgcggaccgc cgtcccgaag ctccaccgcc tcgtaggtgc cggtggacgc gccggagggc
       61 actgcagccc ttccggaagc cccgctgtca agccagacct ccacttccac cgtcgggttt
      121 ccccgggagt ctattatttc tcttccgtgc actcctataa tactgctcac cggtctcagc
      181 tccttcgttc tgttccttcg ttctgttcct tcgtcatccg tccagggatg atgtccttcc
      241 gctaccacgg tttttccgcc ggatccgccc gccgttcctc ccggacgccg taggctcctc
      301 cgggagcgcc ccgcttgagc acgttttcgt catcacagag gatttccact gtcaggattc
      361 tcgaggggta ggctatgtcg aggacgtccc ccgtccggat ctcggcagca ggtttgcact
      421 gccttccgtt gatcctcaca gctccgaggg tgaccatttc ctgcgccacg gtgcgcctct
      481 tgacaaggcg ggcgagtttg agatatttgt ccagtctcat gattcttttt cctccggtag
      541 taaagccatc gaaaagtata tcaaacctgg tccgtatttt tcaattcttt cacaaagtca
      601 ttttgccttt tccgcagggc tgcttttcag cagccgggca acctcccgga accgcaggag
      661 ttcttccctg tccgcctcac cccgggaaag tttttccctg atacgttcat attcccgccg
      721 ggctttcctt ttgccgagaa tgtccaggac aacatcccag gccgcccgtg cgtcaccgag
      781 cccctcgcac cagtctcctc cgcgggcgat ggcccggagg gggaatgtct ccccgaggga
      841 atgccagcgg gcttccacat cctccctgga cggggcggaa agaagggctg ccgccaccgt
      901 ctggatacgc tcgtcctcga caagggaaag aacctgtatt tcgtccgcgg tcctgcggat
      961 atggtcatcc ttccacagca gggcacacac ggcggcctcc acttcgtcac cttcgtcctc
     1021 cctcctcggg gcgacgggcc gttccgtgtc cgcagcggaa tgttcccggt gtcccccccg
     1081 cttccggtat tcttccagca atgcgctcag ttcgtgggga aagatgccca tggaggcggc
     1141 aaccctgggc aggtaggggg ccacgtccac aggggacagg ctgcccaggc ccgatagaat
     1201 ctcgtcggcg gccttgcggc gcttgtcggg agacctgagc atctcgcccc ggaggaagac
     1261 atgatggaga atcagcggcc tggccgaggc cagggccttc tcgaaaagct ccctgccctc
     1321 gggaagggag aggagatcgt cggggtcctt tcccctggga agttccacga ccctgacgtc
     1381 cagccccgct ttctggagca cgtacattcc cctcatggcc gcctcctgcc ccgcgagatc
     1441 ggaatcgtag cagatgcagc accggtccgc caggcgccgt atgagagccg cctgctcttc
     1501 ggtgagggcc gttcccaggg acgcggcggt ctcctcgaat cctcccaggt ggagccggat
     1561 ggcgtccata tagccttcca cgaggatcag c
//
LOCUS       DAOTHD010000571         1590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_92499_length_1590_cov_1.940717, whole genome shotgun sequence.
ACCESSION   DAOTHD010000571 DAOTHD010000000
VERSION     DAOTHD010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92499_length_1590_cov_1.940717"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /gene="dnaJ"
                     /locus_tag="P5219_09290"
     CDS             complement(<1..1022)
                     /gene="dnaJ"
                     /locus_tag="P5219_09290"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRX26995.1"
                     /translation="MYGRGETDDLYQVLGVSRDASAAEIKKAYRQLVRKYHPDANPGN
                     KEAEEHFKKINQAYEVLSDTQKRAQYDQFGTVGDMPGGSPFDGFGGGVGDIFGDIFDN
                     LFGGAGGRRRSNPNAPRRGADLEMETEVTLLEAAEGVTREFQIPRWEPCGECGGSGAK
                     PGTSPETCPSCGGRGQVETKQQTPFGQFVTVNTCPRCNGAGKVVKEKCPSCGGAGRTR
                     KTHRVEVKIPAGVDTGTRLRIAGEGEPGINGGSSGDLYLLVAVAEHPDFKRDGADLHT
                     RLVLSFPQAALGCTVQVAVLSGEPESLSVPAGTQPGRVFTLRGKGIPRLRGARGTEDL
                     HVHVVV"
     gene            complement(1035..>1590)
                     /locus_tag="P5219_09295"
     CDS             complement(1035..>1590)
                     /locus_tag="P5219_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRX26996.1"
                     /translation="VKLGTFVLDGIPPAPRGIPQIEVTFNIDVNGILNVAAKDKGTGK
                     AQNISIHSSNLSKDDIERMKTDAETFEEEDKKKREGAETRNEADSAAYGAERLLSENG
                     DKMTAEEKGAITSAMEEVRRLLPGEDFDAIRSAKSKLEKAVQDFSTRLYSQASAQGDG
                     GNDGGAAAPEGETVDAEFTDQGQA"
BASE COUNT          218 a          550 c          475 g          347 t
ORIGIN      
        1 tccaccacca catggacgtg aaggtcttcc gttcctctgg ccccccggag ccgtggaatt
       61 ccctttcccc tgagggtgaa caccctgccc ggctgtgttc ccgccggaac ggaaaggctc
      121 tccggttcgc ccgaaagaac cgcaacctgg acggtgcagc ccagggccgc ctgggggaag
      181 gagaggacca gcctcgtatg gaggtcggct ccgtctcttt tgaagtcggg gtgctccgcc
      241 acggcgacca gcaggtacag gtctccggag gaaccgccgt tgatgccggg ctctccttct
      301 ccggcgatgc ggagccgtgt gcccgtatcc accccggcgg ggattttcac ctccaccctg
      361 tgggtcttcc gggtcctccc ggcaccgccg caggaggggc atttctcctt gaccaccttt
      421 cccgcgccgt tgcaccttgg gcaggtattc accgtcacga actggccgaa gggagtctgc
      481 tgcttcgttt ccacctggcc cctgccgccg caggacgggc atgtctccgg ggatgtgccg
      541 ggcttcgctc cggagcctcc gcactcaccg cagggctccc accggggaat ctggaactcc
      601 ctggtcacgc cctccgccgc ttccagaagg gtgacctccg tttccatctc gaggtccgct
      661 cccctccggg gggcgttggg gttggatcgt ctccggcccc cggcgccccc gaagagattg
      721 tcgaaaatat cgccgaagat gtcgcccacg ccgccgccga atccgtcgaa aggacttcct
      781 ccgggcatgt cgcccacggt accgaactgg tcgtactggg cccgcttctg ggtatcgctc
      841 aggacttcgt aggcctggtt gatcttcttg aaatgctctt ccgcttcctt gttaccgggg
      901 ttggcatcgg gatggtactt ccgcaccagc tgccggtacg ccttcttgat ctcagccgcg
      961 gaggcgtccc gcgaaacccc gagtacctgg tacagatcgt cggtctctcc ccggccgtac
     1021 atcttcggtc actcctacgc ctggccctgg tcggtgaatt cagcgtccac ggtttccccc
     1081 tccggggcgg ccgctccgcc gtcgttccca ccatccccct gggccgatgc ctgggagtag
     1141 agacgggtcg agaagtcctg taccgccttc tcgagcttcg acttcgccga ccgtatggcg
     1201 tcgaagtcct cgccgggcag gagacggcgc acctcttcca tggcggaggt gatggccccc
     1261 ttctcctcgg cggtcatctt gtcgccgttc tccgaaagga gacgttctgc gccgtaagct
     1321 gcgctgtccg cctcgttccg ggtctccgcc ccttcccttt tctttttgtc ctcttcctcg
     1381 aaggtctcgg cgtcggtctt catccgctcg atatcgtcct tcgacaggtt ggacgaatgg
     1441 atggagatgt tctgggcctt gccggtaccc ttgtccttgg cggccacgtt caggatgccg
     1501 ttcacgtcga tgttgaaggt cacctcgatc tggggtatcc cccggggtgc tggtggaatg
     1561 ccgtcgagaa cgaaggtgcc gagcttcacg
//
LOCUS       DAOTHD010000572         1588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_92616_length_1588_cov_2.961513, whole genome shotgun sequence.
ACCESSION   DAOTHD010000572 DAOTHD010000000
VERSION     DAOTHD010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92616_length_1588_cov_2.961513"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..1003
                     /locus_tag="P5219_09300"
     CDS             152..1003
                     /locus_tag="P5219_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRX26997.1"
                     /translation="MIVDCHVHVYPPEIVRDAEKIARTEEHFALLIGGKVHKWAAAED
                     VVEQMDRDGVDVSWIFGFAFKDPGLCVLCNDYVIDAVRRFPGRFRGLAVVPPMARGMD
                     REIIRCREAGLVGVGEIFPQGQNLDISDIRQTWRLAGTVHELGMFLMFHSAEPVGHDY
                     AGKGNVGPREAAEFCVHHPETRVIFAHFGGGLWMYELMPEMKLYLSNARYDSAAWPWL
                     YEPAVFSAMAAAGVGDKILYGSDFPILSFPRYKKLLDASGASDEAGAQFLSGNALSFL
                     REDHPLS"
BASE COUNT          328 a          410 c          460 g          390 t
ORIGIN      
        1 aggaagaggc cgaagaagtc aagaagaagc tcgtcgaggc cggcgccgac gtcgaagtca
       61 agtagtttct ctttttcggt tttcagctat actgaaggcg gagcattcat tgctccgtct
      121 tattttttta cccttttgcc ggaggtttgc catgatcgtc gactgccatg tccatgtgta
      181 cccgccagaa atcgtccggg acgcggaaaa aatagcccgg acggaggaac atttcgcact
      241 gctcatcggc gggaaggtcc ataaatgggc tgcggccgaa gacgtggttg agcagatgga
      301 cagggatggg gtagacgtat catggatatt cggatttgct ttcaaggatc cggggctgtg
      361 tgtcctgtgc aatgactatg tcatcgacgc agtacgcagg tttccaggcc gtttccgggg
      421 cctcgcggtg gttccgccca tggcgagggg gatggaccgc gaaatcatca ggtgccgcga
      481 agccggtctt gtaggcgttg gcgagatttt tccccaggga cagaatctcg acatttccga
      541 catacgccag acgtggcggc ttgctggtac agtccacgag cttggtatgt tcctgatgtt
      601 ccattcagct gagccggtag gccatgacta tgcgggaaag ggcaacgtcg gcccccggga
      661 ggctgcggaa ttctgtgttc accatcccga aacccgggtg atcttcgccc atttcggcgg
      721 cggattgtgg atgtacgaac tcatgcccga gatgaagctc tatctctcca acgcccggta
      781 tgattccgcc gcgtggccct ggctctacga gccggcagtt ttttccgcca tggctgccgc
      841 cggggtgggg gataagattc tctacggttc cgactttccc attctctcct ttccccgata
      901 caaaaaactc cttgatgctt ccggcgcctc cgatgaggcc ggagctcaat ttctttccgg
      961 aaacgccctt tcttttctca gagaagacca cccactttca taggctgatt catgccctca
     1021 tgcttctgct gaatagaaga cagctgctct gatctcttgt ccgggcggag gagggctatt
     1081 tacgaaaata aagatggata ctatactgca aataatgaat atagaataag gtgtgtcatt
     1141 agggttgtat ccagagagta ttgtgagcag agagggtggg taaaatggca accagaaagg
     1201 catcaggcgc tttcctgtcc ctttttattc tcctgggctt ccttgtcctt tcgctttttt
     1261 cacggcacgc agaaggggcg gtcttccgtg taacccagaa cggggccggg ctgcgcgacg
     1321 gctcgtcgtg ggcaaatgca tgtgacgcag ggagattccc gcagcttctc tcaggcgcat
     1381 ccccgggaac ggagatatgg atagccgaag ggagatacag gccgggcagt ccgaaccgcc
     1441 cctattcagc gttcatactt cctccgggtg tatccttgta cggaggtttt gcaggaaatg
     1501 aagtttcggt ggagcagagg gatccccttg ctcacgtctc agttcttacc ggggatctga
     1561 gccttgatga cactgtcaat tcccatgg
//
LOCUS       DAOTHD010000573         1583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_93036_length_1583_cov_3.454188, whole genome shotgun sequence.
ACCESSION   DAOTHD010000573 DAOTHD010000000
VERSION     DAOTHD010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93036_length_1583_cov_3.454188"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..1343
                     /locus_tag="P5219_09305"
     CDS             30..1343
                     /locus_tag="P5219_09305"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRX26998.1"
                     /translation="MAAAAAISFGVPGTAAAAATVEDGYPAKLKGHPKQAMLTALYSS
                     VTGDLISTLLLVFVAVPMARVALHFGPVEFFALYVFALMMISLLVVGEMKKGIAAAAI
                     GLFIGTIGMDPMMGSVRLSFGISSLRGGIPLIPLLVGVFAVSELMIQFGRECASRSAK
                     KVAEDAKKAEDLGIYDPAKDRMSLKIYRSTFKATLIGSGMGTFIGALPGAGSSLAAFM
                     SYGLARKMSRKPEEFGTGTLEGVAAPEAGNSATAGASLIPLFAFGIPGSATAALFGAA
                     LIMQGINPGPMMIEENKVIIYTLFVIIIYATFINLVLSHGLIPLYAKLAQIKACYLVP
                     TVFALALLGTFASRNSLFDVWVLLAAGVLGIYLRKNDFPLGPLVLGFIVGPGAEKALR
                     QALLMGRGDWMFLLKSPIALGFYALCVISLLLIHFSVKKKLVEED"
     gene            1349..>1583
                     /locus_tag="P5219_09310"
     CDS             1349..>1583
                     /locus_tag="P5219_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX26999.1"
                     /translation="MDTAKTTERKTEPLFRNPSRVSGLLLMAAAAFFFSFTVTGKWSS
                     GAGIGARLFPQLSLAAMFLSGAAIFGRSGEAERN"
BASE COUNT          283 a          525 c          447 g          328 t
ORIGIN      
        1 tcagcttcgc agggtagccg tcctccacgg tggcggcggc ggcggcgatt tccttcggcg
       61 ttccgggcac cgccgccgcc gccgccaccg tggaggacgg ctaccctgcg aagctgaagg
      121 ggcatccgaa acaggccatg ctcacggcgc tgtactcatc cgtcacggga gacctcatca
      181 gcaccctgct cctggtcttc gtggccgttc ccatggccag ggtggccctg cacttcggcc
      241 ccgtggaatt tttcgccctc tatgttttcg cccttatgat gatttcactg cttgtggtcg
      301 gcgaaatgaa aaaaggcatc gccgccgcgg caatcggcct ttttataggg accatcggca
      361 tggaccccat gatgggatcg gtgcggcttt ccttcggcat ttcgagcctc cgggggggca
      421 ttcccctgat tccccttctc gtcggagtgt tcgccgtttc ggaacttatg atccagttcg
      481 gcagggaatg cgcgtcccgc tcggcgaaaa aagtggccga agacgccaaa aaagcggaag
      541 acctcggaat ttacgatccc gcgaaggaca ggatgagcct caagatctac cggtccacct
      601 tcaaggcgac cctcatcggt tcgggaatgg ggacctttat cggcgctctt cccggggcgg
      661 gatcatccct cgcggccttc atgagctacg gtcttgcgag aaaaatgtcc cgcaagccgg
      721 aggaattcgg aacgggaacc ctcgagggag tcgccgcgcc cgaggcggga aacagcgcca
      781 cggcgggggc ctcccttatc ccccttttcg ccttcggcat tcccggcagc gcgacagccg
      841 cccttttcgg agcggcgctc atcatgcagg gcatcaaccc cggacccatg atgatcgaag
      901 aaaacaaggt catcatctac accctgttcg tcatcatcat ctacgccacg ttcatcaacc
      961 tggtcctctc ccacgggctc atacccctgt acgccaaact ggcccagatc aaggcgtgct
     1021 atctcgtccc caccgtcttc gccctggccc tgctcggaac cttcgcctcc cgcaactccc
     1081 tcttcgacgt ctgggtgctc ctggccgcag gagtactggg gatatacctg aggaagaacg
     1141 actttcccct cggtcctctc gtcctgggct tcatcgtcgg cccgggagcg gaaaaggcgc
     1201 tgcgccaggc tcttctcatg ggccggggag attggatgtt cctgctgaaa agccccatag
     1261 cgctcggctt ctatgccctg tgcgtcattt ccctgctgct catccatttt tccgtgaaga
     1321 aaaaactcgt ggaggaggac tgagactcat ggacacggca aaaaccaccg aacggaagac
     1381 ggagccgctg ttccggaatc cctcccgggt ctccggcctg ctgctcatgg ccgccgccgc
     1441 cttctttttc tccttcaccg tgaccggaaa atggagttcc ggcgcaggca tcggcgcccg
     1501 gctctttccc cagctctccc tggccgccat gttcctctcg ggagcggcca tatttggccg
     1561 gagcggggag gctgaaagaa aca
//
LOCUS       DAOTHD010000574         1582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_93097_length_1582_cov_8.022921, whole genome shotgun sequence.
ACCESSION   DAOTHD010000574 DAOTHD010000000
VERSION     DAOTHD010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93097_length_1582_cov_8.022921"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..1425)
                     /locus_tag="P5219_09315"
     CDS             complement(100..1425)
                     /locus_tag="P5219_09315"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HRX27000.1"
                     /translation="MSLKQARKLCVLERLCEGRMSNREAASNLNLSVRQTQRVKSRFS
                     KLGPESLVHGNTGGKPPNYVAEHLRDFVALQAMTRWEGASSSHMSELLLAEKGLHLSA
                     KTMTRILKEHGIKSPFTHKGPKRHRRRERKKRLGEMVQVDASPFDWLGNGSMLALHGA
                     IDDATGKILALWLEPTECLFGYFHVLDRMIRTFGVPGILYSDAHTIFFSPKSGKLSVE
                     EELNGCTAALTQFGKALDTLNILPLKASSAQAKGRVERLWGTLQHRLVVDMRLAGVST
                     IGEANAFLATYPDTHNARFAVEPEREEAAFLPAPSPNDLTYILCKRAFRKASGDSTIS
                     WKGRKLMLLDEKHRQKLLKRGSAIEVLTLLDESDVALYDGVIHHLDVPKDADDRKASL
                     KDTAKKTQKDTEEKTPVIPAPDHPWRRWYGNKPNPRELIHSATSAAKVG"
BASE COUNT          288 a          446 c          477 g          371 t
ORIGIN      
        1 ggagctttta agtcaccggt ctttcgatca acccccatcg gctgtcaacg gacagggaaa
       61 atgtcatggg taaaaggcct gtattggaca gggaaaatgt caccccacct tcgccgcaga
      121 agtagctgaa tgaatcagtt ccctcgggtt tggcttattg ccataccacc tcctccaggg
      181 atgatccgga gcgggtatga cgggagtttt ctcctccgta tccttttggg tcttctttgc
      241 ggtatccttc aaggaggcct tcctgtcgtc ggcgtccttc gggacgtcga ggtggtggat
      301 cacgccgtcg tacagagcga cgtcggattc atccaaaagc gtcaggacct cgatggcgga
      361 cccccgcttg aggagtttct gtcggtgctt ttcatccagg agcatgagct tcctgccctt
      421 ccaggagatg gtggaatctc ctgaggcctt gcggaacgcc cttttgcaga ggatgtacgt
      481 caggtcattt ggggaaggag ccggcaggaa ggccgcttcc tccctttcgg gctccacggc
      541 gaaacgtgca ttgtgtgtgt cggggtatgt agcgaggaag gcgttggcct ccccgatggt
      601 tgaaacgccc gccaggcgca tgtcgacgac gagccggtgc tgcagggtcc cccagaggcg
      661 ttccacccgc cctttcgcct gcgcagaaga ggccttcagc ggcaggatgt tcagggtatc
      721 cagggccttc ccgaactgtg tgagcgccgc agtgcagccg ttgagctcct cctcgacgga
      781 aagcttcccc gattttggag agaagaagat cgtatgggcg tcggagtaga ggatccccgg
      841 gacgccgaag gtgcgtatca tgcggtccag gacgtggaaa tagccgaaga ggcattccgt
      901 cggttccagc cacagggcga gaatcttccc cgtggcgtcg tcgatggccc cgtgcagggc
      961 gagcatggag ccgttgccga gccagtcgaa gggagaggcg tcgacctgaa ccatctcccc
     1021 gagtcgcttc tttcgctccc tgcggcggtg tcttttcggc cccttgtgtg tgaaagggct
     1081 tttgatcccg tgttccttca ggatccgggt catcgtcttc gccgaaaggt gaaggccctt
     1141 ttccgcgaga agcagttccg acatatggct ggagctggct ccttcccacc gggtcattgc
     1201 ctgcagggcg acaaaatccc ggaggtgttc ggccacgtag ttcggcggtt tgccgcccgt
     1261 attgccgtgc acgagcgatt cggggcccag cttgctgaac cgagatttga cccgttgtgt
     1321 ctgccgtaca gagaggttca ggttcgacgc cgcctcccgg ttggacatcc ggccctcaca
     1381 gaggcgttcc aggacacaga gcttccgcgc ttgcttcaat gacatcttga ggtcacccgc
     1441 attcttcttc atgttcggta ttctgactca taggggtgac attttccctg tccccttaaa
     1501 gggtgacatt atcgctgtcc ggtcacacga ggctatggat cagtatgaca attttgccaa
     1561 aaagtgttat tctcttatgc ta
//
LOCUS       DAOTHD010000575         1582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_93140_length_1582_cov_2.860511, whole genome shotgun sequence.
ACCESSION   DAOTHD010000575 DAOTHD010000000
VERSION     DAOTHD010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93140_length_1582_cov_2.860511"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="P5219_09320"
     CDS             complement(<1..261)
                     /locus_tag="P5219_09320"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRX27001.1"
                     /translation="MNERTRKLVDCFFAENTGLEPVRDQVLEAAHVLVRRFLEGGTLL
                     VCGNGGSCADSDHIVGELMKGFLLKRPLPPGERERFSALFGPE"
     gene            complement(279..1436)
                     /locus_tag="P5219_09325"
     CDS             complement(279..1436)
                     /locus_tag="P5219_09325"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRX27002.1"
                     /translation="MKEPGTDGEAGIPAPGADGQAFPFFGVLAAVASLALAVVFPLIK
                     PLAWSVLLSFSVYPLFEKMREGPFRRRSDGFSAAAATGMIVLFAVIPCIFVLVLALRE
                     GVRLHGFVLEVLAAMESTGEGALATLLPEWLVEHIRPWMLRYPAIRGWVRETAARGAV
                     EAVSAAGDAALFLYYVSIITVSCFFLLRDGKYIVSYLREIVPLPPDQAAGFFDRGNRT
                     LQAVVYGVLLTALVQGAAGGLGWLIAGLPNPVLFGALMTFFALIPFLGTAFVWLPGGV
                     YLLAVSDWTGGLILLGWGVLVVGTIGRVLKALFISGGGKIHFFVVFVGIVGGLAAWGI
                     LGLFIGPPVMSLFLFLLENYRITRKTRILHMEAHSGADLASSGAGVYDQEN"
BASE COUNT          375 a          509 c          461 g          237 t
ORIGIN      
        1 ttccgggccg aacagggcgg aaaaccgctc ccgctccccg ggcggcaggg gacgcttcag
       61 aaggaacccc ttcatgagct cccccacgat atggtcactg tcggcacagc ttcccccgtt
      121 gccgcagaca agaagcgtac cgccctccaa aaatcggcgg accagcacat gggcggcctc
      181 gagcacctga tccctaacag gttccaggcc ggtgttttca gcaaaaaaac agtcaacgag
      241 cttccgggtc cgttcgttca tgccttcctc cgggaacgtc agttttcctg atcatacacc
      301 ccggcaccgg aagaggccag atccgctccg gaatgggcct ccatgtgcag aatccgggtc
      361 tttcgcgtga tcctgtagtt ttcaagaaga aaaagaaaga ggctcatcac gggcggtccg
      421 atgaaaagac cgaggattcc ccaggcggca aggccgccca cgatgcccac gaagaccacg
      481 aagaagtgga tctttcctcc gccggaaatg aaaagggcct tcaatacccg gccgatggtt
      541 cccaccacca gaacgcccca tccaagcaga atgaggccgc cggtccagtc cgaaactgcc
      601 agcaggtaaa ccccaccggg aagccagaca aaagctgtcc cgaggaaggg aatcagggca
      661 aaaaacgtca tgagggcgcc gaagagcacc gggttgggaa ggcccgcgat gagccacccg
      721 agtccaccag ccgcaccctg caccagggcc gtgagcagga caccgtacac caccgcctgg
      781 agcgtccgat ttccccggtc gaaaaagccc gcggcctgat ccgggggaag ggggacgatt
      841 tcccgcaggt aggagacgat gtactttccg tcccggagaa gaaaaaagca ggatacggtg
      901 atgatggaaa cgtagtagag aaacagcgcc gcgtctcccg ccgccgacac cgcctccact
      961 gcgccccggg ccgccgtctc ccgtacccac ccccggatcg ccggataccg gagcatccac
     1021 gggcggatat gctccacgag ccactcggga aggagggtgg ccagggcccc ttcaccggtg
     1081 gattccatgg cggccagaac ttcgaggaca aatccgtgga gacgtacacc ttcccgcagg
     1141 gccaggacga ggacaaagat gcagggaatg acggcgaaca ggacgatcat ccccgtggca
     1201 gcggcggcgg aaaagccgtc ggaccgcctg cggaacggcc cctccctcat tttttcgaac
     1261 aggggataga ctgaaaagga aaggagcacc gaccaggcga ggggcttgat cagcggaaag
     1321 accacggcaa gggcaaggga ggccactgcc gctaaaacgc cgaaaaaggg aaaagcctgt
     1381 ccgtccgccc cgggtgccgg tattccggcc tcgccgtccg ttcccggttc tttcatgaac
     1441 gccacatcct ttccgtgaaa gaatgaaaaa gcctcccgga ttcctccggg aggcttcact
     1501 ataccatttc ttcattccct cggggggatg gattctagta catatcctcc atgccgccca
     1561 ttccgcccct gggcatggcg gg
//
LOCUS       DAOTHD010000576         1582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_93158_length_1582_cov_2.434840, whole genome shotgun sequence.
ACCESSION   DAOTHD010000576 DAOTHD010000000
VERSION     DAOTHD010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93158_length_1582_cov_2.434840"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="P5219_09330"
     CDS             <1..657
                     /locus_tag="P5219_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRX27003.1"
                     /translation="ADVVGGIPWIEYTDADMQEHIDRMFALAKEFNKDVSMLIDDAGD
                     PGLRSLEMLAVKTLKEGWQGRVTAQHCRAMAMYREPYFRKILALLQKASIGLVSDPQT
                     GPLHARVRDLYDSGVAVALGQDDIADAYYPFGRNNMLEVAFLAVHLLWMTTFSDMEII
                     YDLITTNAAKAMGIRGHVLEPGGNADLVVLNANDVYHAIWEHEAPFRVIRKGQDVTLK
                     "
     gene            complement(964..>1582)
                     /locus_tag="P5219_09335"
     CDS             complement(964..>1582)
                     /locus_tag="P5219_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HRX27004.1"
                     /translation="LRLLRDRPGLSSRELSRRIVSEALVREPHEHAGDDVTCAVMYFR
                     SPRKLLVLSGPPFDGQRDREYAEMLGKFPGRKAICGGTTAEIVARELGRPLVTDLATA
                     RGGMPPLSSMDGVDLITEGILTLTLVSKILDGKEIVREKNSAARLASLLLESDRISFV
                     VGTRVNEAHQDPTLPVELEMRRNIIRTIVRTLEEKYLKEVTVSFI"
BASE COUNT          313 a          524 c          484 g          261 t
ORIGIN      
        1 gccgacgtcg tgggcggcat tccgtggatc gagtacaccg acgccgacat gcaggagcac
       61 atcgacagga tgttcgccct ggccaaggag ttcaacaagg acgtctccat gctcatcgac
      121 gacgccggcg acccgggcct caggtccctc gagatgctgg cggtgaaaac gctgaaggaa
      181 ggctggcagg gacgggtcac cgcccagcac tgccgggcca tggccatgta ccgggagccc
      241 tacttccgca agatcctggc cctgctccag aaggcctcca tcggcctggt gagcgatccc
      301 cagaccggcc ccctccacgc ccgggtccgg gacctctacg actccggcgt ggccgtcgcc
      361 ctcggccagg acgacatcgc cgacgcctac taccccttcg gcaggaacaa catgctcgaa
      421 gtggccttcc tggcggtcca cctgctgtgg atgaccacct tcagcgacat ggagatcatc
      481 tacgacctca tcaccaccaa cgcggcgaag gccatgggca tcaggggaca cgtcctcgag
      541 cccggcggga acgccgacct ggtggtgctg aacgccaacg acgtctacca cgccatctgg
      601 gagcacgagg cccccttcag ggtcatccgg aagggacagg acgtcaccct gaaataaccc
      661 ctccgaagca cggcatcccc ctcccgaagg agggggatgc tttttcaggc cttttttaaa
      721 aaacagcggg agtgttgccg ggcataaggt gaattcgcca actccagggg cgaagagaca
      781 atcccctggg ggacagcgcc atgccgagaa accgacaagg atgtcggttt cacaagcagg
      841 caaccggcga acgaaggtag ccgagttgat cgcttgagca gttgtcacgg atgacaatgt
      901 gcgccggcgg cccaagcgag caccggtaac gctccctgct ggaaaaaact gcggattttc
      961 ccttcaaatg aacgaaacgg tcacttcctt caggtacttt tcctccaggg tgcggacgat
     1021 ggtccggatg atgttccggc gcatttccag ctccaccggc agggtggggt cctggtgggc
     1081 ctcgttcacc ctcgtcccca ccacaaagga aatccggtcg ctctccagca gcagggaggc
     1141 cagcctcgcc gccgagttct tctcccggac gatctccttt ccgtccagga tcttggacac
     1201 cagggtcagg gtgaggatcc cctcggtgat gaggtccacc ccgtccatgg aggacagggg
     1261 cggcattccc ccccgggccg tggcgaggtc cgtcacaaga gggcgtccca gctccctggc
     1321 cacgatttcg gcggtggtgc cgccgcagat ggccttcctt ccagggaatt tcccgagcat
     1381 ttcggcgtac tccctgtccc gttgcccgtc gaagggaggg cctgaaagca ccaggagctt
     1441 ccggggcgac cggaaataca tgacggcgca ggtcacgtcg tcaccggcat gctcatgggg
     1501 ctccctgacc agtgcctcgg agacgatgcg cctcgacagc tcccgggaag agagcccggg
     1561 cctgtcccga agcagacgca gg
//
LOCUS       DAOTHD010000577         1580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_93324_length_1580_cov_2.537705, whole genome shotgun sequence.
ACCESSION   DAOTHD010000577 DAOTHD010000000
VERSION     DAOTHD010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93324_length_1580_cov_2.537705"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="P5219_09340"
     CDS             complement(<1..855)
                     /locus_tag="P5219_09340"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRX27005.1"
                     /translation="MHGFLVILPVVLVMAAGRMLARRGVVSPGAFAQINKAIYWTAIP
                     ALILRMTSMADMSALADRNMVVAVYLSFLAAPPAAWLIGKLAGQERRRTASSTLMVIR
                     SNTVFVGLPVVTVAVGTRGVEVLSLYLAFTFIGYQIISISWAQLALSGGISWNTVKGT
                     LKNMAKNPLVLSSLAGFTLAVTGLNSFPVWLDETLKLLGNIASGTALLSLGATLKLEA
                     LTSMLPRAWRDVILKLIFLPALTWGLFLLFPVNPLVFRTVVLIAAMPVAVDCFILSQV
                     LEMDPDQSA"
     gene            complement(860..>1580)
                     /locus_tag="P5219_09345"
     CDS             complement(860..>1580)
                     /locus_tag="P5219_09345"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRX27006.1"
                     /translation="AEAGSVVIDNSSAFRMDPAIPLVVPEINGDLLKGYRSGIVANPN
                     CSTIQMVLALYRVHERYGIRSIVVSTYQSVSGAGKKGMNELEDQMKGHIAPKKFVKQI
                     HLNVVPQIGSLLEDGFTDEEMKMVNETRKILRDPDISVWPTTVRVPVLYCHSESILTE
                     TRKPFDLQGIEAALEAGEHVALSRDMFTPLDAAGTDLTYVGRVRAFDDTRFLMWNVAD
                     NIRVGAATNAVRILLKHGELN"
BASE COUNT          300 a          489 c          519 g          272 t
ORIGIN      
        1 cgctgactgg tcagggtcca tttccagcac ctgggacagg atgaagcagt ccactgccac
       61 gggcatggcg gcgatgagga ccaccgtgcg gaagaccaac ggattcaccg ggaacaggag
      121 aaaaaggccc caggtaaggg ccggcaggaa gatcagcttc agaatcacgt ccctccaggc
      181 cctgggcagc atggaggtca gcgcctcgag cttcagggtc gccccgaggg agaggagggc
      241 cgtgccgctg gcgatgtttc ccaggagctt cagggtctcg tccagccaca cgggaaaaga
      301 gttcagcccc gtcaccgcca gggtgaaccc tgcgagactc gacagcacca gggggttctt
      361 cgccatgttc ttcaaagtgc ccttcacggt gttccaggag atgccccccg acagggcgag
      421 ctgggcccag gagatggaaa tgatctggta gccgatgaag gtgaaggcca ggtagaggga
      481 cagcacctcc accccccggg tccccaccgc cacggtgacc accggaaggc ccacgaacac
      541 agtgttggac cggatgacca tcagggtgga cgacgcggtg cgcctccgct cctgcccggc
      601 gagcttcccg atcagccagg ccgctggcgg ggcggcaagg aaggagaggt atactgccac
      661 caccatgttc ctgtccgcaa gggcggacat gtcggccatg ctggtcatcc ggaggatgag
      721 ggccggtatg gccgtccagt agatggcctt gttgatctgg gcgaaggctc cgggagacac
      781 gaccccgcgg cgggccagca tccttccggc agccatgacc agcaccaccg gaaggatgac
      841 gagaaagccg tgcatggacc tagttcagtt ccccgtgctt caggaggatc ctcaccgcgt
      901 tggtggccgc tcccacccgg atgttgtccg ccacgttcca catcaggaat cgggtatcgt
      961 cgaaggccct gacgcgcccc acgtacgtga ggtccgtacc ggccgcgtca aggggggtga
     1021 acatgtcccg tgaaagggcc acgtgttcgc ccgcctccag cgccgcctcg attccctgaa
     1081 ggtcgaaggg ctttcgtgtc tccgtgagga tgctctcgga gtggcagtag agcaccggaa
     1141 cacgcacggt ggtgggccag acggagatgt cggggtcccg gaggatcttc cgggtctcgt
     1201 tcaccatctt catttcctcg tcggtgaagc cgtcctcgag cagggagccg atctggggca
     1261 ccacgttgag gtggatctgc ttcacgaact tcttcggggc aatgtgcccc ttcatctgat
     1321 cctcaagctc gttcatgcct tttttgcccg ccccggagac ggactggtag gtgctcacca
     1381 cgatggaccg gatgccgtac cgctcatgga ccctgtacag ggcgaggacc atctggatgg
     1441 tggagcagtt cgggttggcc acgatgccgc tgcggtagcc tttcaggagg tcgccgttga
     1501 tctcgggcac caccaggggg atggccggat ccatgcggaa ggcggacgaa ttgtcgatga
     1561 ccacgctccc cgcctccgca
//
LOCUS       DAOTHD010000578         1577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_93519_length_1577_cov_3.861367, whole genome shotgun sequence.
ACCESSION   DAOTHD010000578 DAOTHD010000000
VERSION     DAOTHD010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93519_length_1577_cov_3.861367"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="P5219_09350"
     CDS             <1..369
                     /locus_tag="P5219_09350"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRX27007.1"
                     /translation="GILSDSFAAAVCFRLNVPEKKLDYCAAGINGFLYGSGAGLKKII
                     TPGSLLGISGTALFGSGSLPVEPGEYFLFLSDGFLDQQSVRENEHLSFSGAYDRLFQV
                     GYTGAARDDQSALCVLFRDS"
     gene            complement(366..>1577)
                     /locus_tag="P5219_09355"
     CDS             complement(366..>1577)
                     /locus_tag="P5219_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX27008.1"
                     /translation="QEDQARRITELEEALRRAEESSRNKSAFIANLSHEIRTPLNSII
                     GVANLFQGTRLDLEQQKFVEMLSTSARDLHRLVEELLDLSLIETGRLTLQSDLFPVRS
                     TCSRTVRPLAVSVQERRLSLELHVDPAVPELLLGDSARLGQILRNMVTNAIAFTPKGT
                     ISVRVFSGEEKESAVILHITAGHPGGGAGTEKRRDTDYSNMERKDFSASFQGAGMGLL
                     IARGIAEAMGGKLWTELSGSGDRVIHFRASLGKIPPGETFRKKDGDSGKELPADGAPL
                     PEKISILVVDDNRFNRSLARTILRKMGGPGWDVFLAESGSEALQLLEEKHYDLVFMDV
                     QMPDMDGLECTRLARAKEAVRGLRSVIIAMTAYAMEGDRRMCLDAGMDDYIAKPVEAE
                     VLRSVISRNLA"
BASE COUNT          281 a          482 c          464 g          350 t
ORIGIN      
        1 ggcattctct ccgattcttt tgctgcggcg gtctgcttcc ggctgaacgt tcccgaaaaa
       61 aagctggact actgcgccgc cggaatcaac ggtttcctgt acggcagcgg agccggactg
      121 aaaaagatca ttactcccgg atcgctgctg gggatttccg ggactgccct tttcggttcg
      181 ggcagcctcc ccgtggaacc tggcgagtac tttcttttcc tgagcgacgg attccttgac
      241 cagcagagtg tgcgggaaaa cgagcatctt tccttttccg gggcctacga ccgcctgttc
      301 caggtcggct acacgggggc ggcccgggac gaccagtccg cactctgcgt gctgttcaga
      361 gacagctaag caaggtttct ggagatgacg gagcggagca cctcggcctc gaccggcttg
      421 gcgatgtagt catccattcc cgcgtcgagg cacatccgcc ggtctccctc catggcgtag
      481 gccgtcatgg caatgatgac ggatctgagg ccccggacgg cctctttcgc cctggccagc
      541 cgcgtgcatt cgaggccgtc catgtccggc atctggacat ccatgaagac aagatcgtaa
      601 tgcttttcct ccaagagctg cagcgcctcg gagccgcttt ccgccaggaa cacatcccag
      661 ccgggaccgc ccattttcct gagaatggtc ctggcgagac tccggttgaa cctgttgtca
      721 tccaccacca ggatggaaat cttctccgga aggggggctc cgtctgcagg aagttccttt
      781 ccggaatctc cgtctttttt cctgaatgtc tccccgggag gaatctttcc gagggaagcc
      841 cggaaatgaa tgacccggtc gccggagcct gacaactccg tccaaagctt tccgcccatg
      901 gcttcagcta tcccgcgggc aatgagaagc cccatgccgg ccccctggaa ggatgcggaa
      961 aaatccttcc gttccatgtt cgaatagtcc gtgtcccgcc ttttctccgt cccggcccct
     1021 ccgccgggat ggccggcggt gatgtgaagg attactgccg attctttttc ctccccggag
     1081 aataccctga ccgaaattgt tcctttgggt gtaaaggcga tggcgttggt caccatgttc
     1141 ctgaggatct gtcccagccg cgcgctgtcc cccaggagca actccggaac ggcggggtca
     1201 acgtgcagtt ccagggaaag ccgccgttcc tggaccgaaa cggccagggg ccgaaccgtc
     1261 ctggaacagg ttgaccggac gggaaagaga tcgctctgca gcgtgagcct tcccgtttca
     1321 atgagggaaa ggtcgagcag ttcctcaacg aggcggtgca gatccctcgc cgatgtggag
     1381 agcatttcga cgaatttttg ctgctccagg tccagcctgg tcccctggaa gaggtttgcc
     1441 acgccgatga tggagttgag aggtgtgcgt atttcgtggc tgagattggc gatgaaggcg
     1501 ctcttgttcc tgctgctctc ttcggccctg cggagggcct cctcgagttc cgtgatccgc
     1561 cgggcctgat cttcctg
//
LOCUS       DAOTHD010000579         1576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_93642_length_1576_cov_2.606838, whole genome shotgun sequence.
ACCESSION   DAOTHD010000579 DAOTHD010000000
VERSION     DAOTHD010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93642_length_1576_cov_2.606838"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1243)
                     /locus_tag="P5219_09360"
     CDS             complement(<1..1243)
                     /locus_tag="P5219_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27009.1"
                     /translation="MQKEATPRFRGGTCVFPRHLRTLLERIPDHPLTVVEAPSGFGKT
                     TAVREFLGEPVRVNSRRNWYTCFGERASKAWEGFCRLFGNVDMEAARKLKKLFPPTVE
                     ILPDIAAILSQCRCDVETYLVIDNYQLFETDIPCGLIGAFSAHGNENLHIVFITQPLP
                     SFGGDFRHSGHIHRLDTRDFFFDRESTARLCRLSGANVSESDVDYIQSVSEGWVSAIL
                     LQASNFRETGSFARVPDMEGLLETAVWDRMSGENRTFLMGVSLLDAFTPRQGALAGGW
                     TTLPEDVTRMLRSSFFISYIADKGAYSIHSILREYLRKQFENQPPALADLLVRRAGDA
                     CRDASDYFEAAKFYMKLRDCDAILSLPFSGTYLNEQKEKEIISFLERLVSDCSEETLR
                     RYPFTLLLFAFHFLKSGMRDRF"
     gene            complement(1269..>1576)
                     /locus_tag="P5219_09365"
     CDS             complement(1269..>1576)
                     /locus_tag="P5219_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27010.1"
                     /translation="MDLSHYKTPEAMAAEDRRTLDVLAGLRPAETKTIDADARRAAAL
                     VLMVFREFGIEKGTVLERTDSSSILGRRVESGTSYATILYGVAEPKEAVSAGKTSLF"
BASE COUNT          311 a          449 c          481 g          335 t
ORIGIN      
        1 agaaccggtc ccgcatgccg cttttcagga aatggaaggc gaagagaagg agggtgaagg
       61 gatatctccg gagcgtctct tcggaacagt ccgaaacgag ccgttcaagg aagctgatga
      121 tctctttctc cttctgttca ttgaggtatg tgcccgagaa aggcagggag aggatggcat
      181 cgcagtcccg gagcttcatg tagaatttcg ccgcctcgaa gtagtccgag gcatccctgc
      241 aggcatcccc ggcccttcgg accaggagat ccgcaagggc tggaggctgg ttctcgaact
      301 gtttccggag gtactccctc agaatgctgt gaattgaata ggcgcccttg tcggcgatgt
      361 aggagatgaa aaagctgctt ctcagcatcc tggtcacgtc ctcgggcagg gtggtccagc
      421 cgcccgcgag ggccccctgc cggggtgtaa aagcatcgag cagggacact cccatgagga
      481 aagtgcggtt ctcacctgac attctgtccc atacggccgt ttcgagaagg ccctccatat
      541 ccggcacccg ggcgaaagac cccgtttccc tgaagtttga tgcctggagc aggatggcgg
      601 agacccatcc ttcggaaaca ctctggatgt agtccacgtc gctttccgag acgttcgctc
      661 ccgacagtcg gcagaggcgg gccgtggact cccggtcgaa gaagaaatcc cttgtatcca
      721 gccggtggat gtgcccgctg tggcggaagt ccccgccgaa agaaggaaga ggctgggtga
      781 tgaacactat atggaggttt tcattgccat gggccgagaa ggcccctatg aggccgcagg
      841 ggatgtccgt ttcaaagagc tggtagttgt cgatcacaag gtaggtttca acgtcgcagc
      901 ggcattggct gagaatagcc gcgatatccg gaagaatttc caccgtgggg ggaaagagtt
      961 tcttcaactt ccgggcagcc tccatgtcca cgttcccgaa aagccggcag aatccttccc
     1021 atgctttcga agccctttcg ccgaaacagg tgtaccagtt ccgcctgctg ttcacccgaa
     1081 ccggctcccc caggaattca cggacggcgg tggtttttcc gaatccggag ggcgcctcca
     1141 ccaccgtgag gggatggtcg ggaattcttt cgagcagtgt tctgaggtgc cttgggaaga
     1201 cacaggtccc gccccggaac cggggggttg cctctttctg cattggtggc cctccaatct
     1261 tgcctttttc agaacaggct tgtttttccg gcggagactg cttcctttgg ttccgccacc
     1321 ccatagagta tggtggcgta gctcgttccg gactctaccc tgcgtcccag gatggatgac
     1381 gaatctgtgc gctcgaggac agtccctttt tcaatcccga actcgcggaa gaccatcagc
     1441 accagggcag cggcacgacg ggcatccgcg tcgatggtct tcgtttcggc cgggcgaagg
     1501 ccggccagga cgtccagggt cctccggtcc tcggcggcca tggcctccgg tgtcttgtaa
     1561 tgggacaggt ccatgc
//
LOCUS       DAOTHD010000580         1575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_93757_length_1575_cov_2.285526, whole genome shotgun sequence.
ACCESSION   DAOTHD010000580 DAOTHD010000000
VERSION     DAOTHD010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93757_length_1575_cov_2.285526"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="P5219_09370"
     CDS             complement(<1..752)
                     /locus_tag="P5219_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27011.1"
                     /translation="MKDLFEKTNFLLDGGVLVLLSEYLADRISRGLIARPDGNDLAVL
                     DAFGTPMNSLFLEGAAGVVDSAFPSPPLRTSFDVLTAPLSGEWPWAVRLRGMGWRFYI
                     LLGEEPTAEILEEMTSFAGLINLWQLHQKTAGVEERLSRLAYMVLATKNTLASIFEPM
                     GVNYFGAFLHDVVQESLFPSRFSLLLDNGETLSLLEGEEMLLPPRKGIFARDILSPVP
                     VRMEESQRALLGDSVFDSLADDWSALLPIPGGT"
     gene            906..1322
                     /locus_tag="P5219_09375"
     CDS             906..1322
                     /locus_tag="P5219_09375"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRX27012.1"
                     /translation="MFLKGRKENPGSVPLPGEEKRRFFRVECVLRTEFRLRDHGSWVP
                     AEVLDLSVAGMKVRFDPFFRGKTLSPERFEWADSIFRFPLNGSFFQIEGCFLRVYLRE
                     AGRFTAGVEFSDPSPEEQFRLVELYGMFRRRSAGIL"
     gene            1384..>1575
                     /locus_tag="P5219_09380"
     CDS             1384..>1575
                     /locus_tag="P5219_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27013.1"
                     /translation="MNTVRGRILASFLALGLLLLGGVSFVALRQSERGLLELAEQEGA
                     ALTRTLTEGFDQYLTASADE"
BASE COUNT          338 a          421 c          499 g          317 t
ORIGIN      
        1 gtccctccgg ggatggggag aagggcggac cagtcatcgg caagggagtc gaaaacgctg
       61 tccccaagga gcgccctttg tgattcttcc atccgcacag ggaccggcga aagaatgtcc
      121 cgggcgaaaa tacccttcct cggtggcagg agcatttcct ccccttcgag gagcgacagg
      181 gtttcgccgt tgtcgagcag cagggaaaaa cgggagggga agaggctttc ctgcaccacg
      241 tcgtggagga aggcgccgaa gtagttcacc cccatgggct cgaagatgga ggcgagggta
      301 ttcttcgtgg ccaggaccat gtaggccagc cgcgagagcc gctcctccac gccggcggtt
      361 ttctggtgga gctgccacag gttgatgagc ccggcgaagg aggtcatctc ctcaagaatc
      421 tccgcagtcg gctcctcccc aaggaggatg tagaagcgcc accccatccc cctgagccgt
      481 accgcccacg gccattctcc cgaaagaggc gcggtgagca catcgaagga tgtccggagg
      541 ggaggggacg gaaaggccga atcaaccacc ccggcggctc cttcgaggaa caggctgttc
      601 atgggtgtgc cgaaggcatc gagcaccgcc aggtcattcc catcaggacg ggctatcaat
      661 ccacgggaga tccggtcggc gagatattcc gaaagaagca caagaacgcc gccgtccagt
      721 aaaaaattgg tcttttcgaa aaggtctttc ataagcggtc aactcccctt tataaaggga
      781 aggaatgagt cttacccttc cctatagcgg aaaaactgct tcaatggtat cgtaaagaca
      841 gaaaaggcgc cactacaatt tgatgacgtc acagggcgtt gtccggatct gcggggtgtt
      901 tgtctgtgtt tttgaagggc agaaaagaga atccggggtc cgtccccctt ccgggtgagg
      961 agaagaggcg cttttttcgg gtggagtgcg tcctccggac ggaattccgg cttcgggacc
     1021 acggatcctg ggtgccggcc gaagtgctgg atctcagcgt ggcgggaatg aaagtccggt
     1081 tcgatccctt cttcaggggg aaaacccttt cccccgaacg gttcgaatgg gcggacagca
     1141 tttttcgttt ccccctgaac gggagttttt tccagattga aggctgcttt ctccgggtct
     1201 acctgagaga ggcgggccgc ttcaccgcag gcgtggaatt ttcggatccc tcgccggagg
     1261 agcagttcag gcttgtggag ctttacggga tgtttcggag gagaagcgcc ggcatcctgt
     1321 gaaaggaacg gaaccgttca atagattgca ctgttgttca acttcgcgaa ggaggtcgta
     1381 ataatgaaca cggtcagagg acggattttg gcttcgtttc ttgcacttgg gctgctgctt
     1441 ctcggagggg tgagcttcgt ggcgctccgc cagtccgaac ggggacttct ggaacttgct
     1501 gaacaggagg gagcggccct tactcggacg ctcacggaag gattcgacca gtacctcacg
     1561 gccagcgccg acgag
//
LOCUS       DAOTHD010000581         1574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_93799_length_1574_cov_3.512837, whole genome shotgun sequence.
ACCESSION   DAOTHD010000581 DAOTHD010000000
VERSION     DAOTHD010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93799_length_1574_cov_3.512837"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="P5219_09385"
     CDS             complement(<1..742)
                     /locus_tag="P5219_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRX27014.1"
                     /translation="MGESSAAQAAPWETSGENVRYVPLDDLCHYRGRVAEDILSEDGA
                     LLLPRGSDIPAILESMPGITKTLRRWNREFIPVQVSSDITEKEFEAVLRAIEPKTRTL
                     DPNLAMRAIHQVEEVYSRISEDGNSREGIETLAREADRLAREVVQTPQILLCLGKVKD
                     SDEYTFIHSLNVALLSGYLASILHPGDEELVKTMTFGGLLHDLGKAKVPPEILNKPGR
                     LTSEEFEVMKTHAIQGMVSAVASGVDDRR"
     gene            complement(745..1566)
                     /locus_tag="P5219_09390"
     CDS             complement(745..1566)
                     /locus_tag="P5219_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRX27015.1"
                     /translation="MIHQLGSAYFANTLMNSFMAQLDEVLVNRVKAVEGEIAETSERF
                     VQLSRILGDLQAEFDRSGRETRETVEEINRMNGDLSAELQRSGTDLDGMSENVSRTVD
                     ATTGTLESFLEIEKMSKEIQRIAKQTNLLALNASIEAARAGEHGRGFAIVAKNVQELA
                     AETKAASESIGAKVGEISGSVERAMEDIRGISDMFDMIRRSLTTFTRFLDTNKAFMER
                     MENTMADAGEKVNRSSGEMESSVAVMENASRKFGSMAATISAIVLAQKNLKKIRL"
BASE COUNT          252 a          504 c          462 g          356 t
ORIGIN      
        1 tccgccggtc gtccactccc gaggccacgg cggagaccat tccctggatg gcgtgggtct
       61 tcatcacttc aaactcctcg gaggtcagcc tgccgggctt gttcagtatc tccgggggaa
      121 ccttcgcctt tccgaggtcg tggagcaggc cgccgaaggt catggtcttc acgagctcct
      181 cgtcgccggg gtggaggatc gaggccaggt accccgacag gagggccacg ttgagggagt
      241 ggataaaggt gtattcgtcg gaatccttca cctttccgag acagagcagg atctggggcg
      301 tttgaacaac ttcccgggca agcctgtcgg cctccctggc aagggtttcg attccctccc
      361 gtgaattccc gtcctccgaa attctggagt atacttcctc gacctggtgg atggctctca
      421 tcgccaggtt tgggtcaagc gtgcgtgttt tcggctcgat ggcccggaga acagcttcga
      481 attccttctc ggtgatgtcc gaagatacct ggacggggat gaattccctg ttccagcgtc
      541 ggagcgtctt cgttatgccg ggcatggact cgaggatcgc cgggatgtcg cttccccggg
      601 gcaggagcag cgccccgtcc tcggagagga tgtcctcggc caccctgccc ctgtaatggc
      661 aaaggtcgtc caggggaacg tatctcacgt tctcaccgga agtttcccat ggtgccgcct
      721 gcgccgcgga tgactctccc atgttcagag ccgaatcttc ttgagattct tctgggccag
      781 gacgatggcg gagatggtgg ccgccatgga gccgaacttc ctggacgcgt tctccatcac
      841 cgccacggag ctctccatct ctcccgagct cctgttgacc ttttccccgg cgtccgccat
      901 ggtgttctcc attctttcca tgaaggcctt gttggtgtcg aggaaccggg tgaaagtcgt
      961 cagggagcgg cggatcatgt cgaacatgtc gctgatcccc cggatatcct ccatggcccg
     1021 ctccaccgaa ccggagatct cccccacctt ggcgccgatg gactcggaag ccgccttggt
     1081 ctcggcggcg agctcctgga cgttcttcgc cacgatggcg aatccccttc cgtgctctcc
     1141 cgccctggca gcctcgatgg aggcgttcag ggccaggagg ttggtctgct ttgctatcct
     1201 ctggatttcc ttcgacatct tctcgatctc aaggaaagat tccagggtgc cggtggtggc
     1261 atccaccgtc ctggacacgt tctcgctcat gccgtcaaga tcggtgccgg agcgctgaag
     1321 ctccgccgac aggtcgccgt tcatccggtt gatctcctca accgtttccc tggtctcccg
     1381 gccgctccgg tcgaactcgg cctggagatc tcccaggatg cggctgagct gcacgaaccg
     1441 ctccgacgtc tcggctatct ccccctccac cgccttcacc cggttcacca gcacctcgtc
     1501 gagctgggcc atgaaggagt tcatcagcgt gttggcaaag taggcactgc cgagctggtg
     1561 gatcacttcc gaat
//
LOCUS       DAOTHD010000582         1573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_93924_length_1573_cov_2.722003, whole genome shotgun sequence.
ACCESSION   DAOTHD010000582 DAOTHD010000000
VERSION     DAOTHD010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93924_length_1573_cov_2.722003"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="P5219_09395"
     CDS             complement(<1..173)
                     /locus_tag="P5219_09395"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HRX27016.1"
                     /translation="MEIYRGITVSIAGSDSGGGAGIQADLKTCAALRVFGMTVITALT
                     AQNSREVSAVWNVP"
     regulatory      complement(247..354)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(375..1055)
                     /gene="pssA"
                     /locus_tag="P5219_09400"
     CDS             complement(375..1055)
                     /gene="pssA"
                     /locus_tag="P5219_09400"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HRX27017.1"
                     /translation="MVTSGNLLCGMMALFLLFHGRFVPAAWLVFMAVFFDCMDGKVAR
                     SLGGGTQFGLEFDSLADVVSFGVVPAMLMYVSYADALGLWGVLAASFFALCGALRLAR
                     FNVVHMPGPFQGLPIPAGGLFLASFILAPVPLHPAAAAFLCFGTGALMISSVPYGSLK
                     GLRKGQGNRKKFLLLAGILALTFVSLQSAAPLAVMAVYVVSGLIRFDWEKWLSRETGE
                     GERAEKKA"
     gene            complement(1096..>1573)
                     /locus_tag="P5219_09405"
     CDS             complement(1096..>1573)
                     /locus_tag="P5219_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HRX27018.1"
                     /translation="PDGPGFLSPADGKVVEVMETDHPFTGKAVKVGIFMNPLDVHVNR
                     VPCDGTVEWMEYIPGRKWMAFEPKASELNERMCVGLATGYGPVLLVQVAGFLARRIVC
                     RLRKGDRLVRGDRFGMIKMGSKVDVYLPAGVQMKTSVGKKVFAGRTVLGGMAGEKS"
BASE COUNT          334 a          518 c          432 g          289 t
ORIGIN      
        1 gggacattcc agaccgcgct tacctcccgg ctgttctggg ccgtcagggc cgtaatgacg
       61 gtcatgccga acacccggag ggcggcacag gttttcaggt ccgcctgtat tcccgcccct
      121 ccgccggaat cgctcccggc gatggagacg gtgattcccc tgtagatttc catgattcca
      181 cctccaaata aatcaggagc cccttcattc cgcggggctc ctgtcttttt tcgtcctccc
      241 ggaatcccgt tccggtgatg acactccctc cgccggcatt acccggatca ggttaggggt
      301 cgaaggccca atcagccttc ctctcagccc gcagcagctc cccccggcat tcagttgtca
      361 ggcggtgcag gacctcaggc tttcttttcc gccctttcgc cctccccggt ttcccgggaa
      421 agccactttt cccagtcgaa ccggatcagt ccgctgacca cgtagacggc catgaccgcc
      481 aggggcgcag cggactggag ggagacaaag gtcagcgcca ggattccggc gagaagaagg
      541 aattttttcc tgttcccctg accctttctc agtcctttca ggctgccgta gggcacactg
      601 gagatcatga gggctccggt tccgaagcac agaaacgcgg ccgctgcagg gtgcaggggt
      661 acaggggcga gaataaagga tgcgagaaag agtccccccg ccgggatggg aagtccctgg
      721 aaaggaccgg gcatgtgaac cacgttgaac ctggccagcc gcagggcacc gcacagggcg
      781 aagaacgacg ccgcaagaac gccccacagg ccgagagcat cggcgtagga aacgtacatg
      841 agcatggcgg gaaccacgcc gaagctgacc acgtccgcca ggctgtcgaa ctcaagtccg
      901 aactgcgttc cgccgccgag gctcctggct accttcccgt ccatacagtc aaaaaaaacg
      961 gccatgaaga caagccacgc agcgggaaca aaccgcccgt gaaacagaag aaatagtgcc
     1021 atcatgccgc acagcaggtt cccgctggtc accatattgg gcgcgatctt ccggaaagga
     1081 agactacccc gctttctacg atttctcacc ggccatccct ccaagaacgg ttctgcctgc
     1141 gaaaaccttc tttcccacag aggtcttcat ctgtactccg gcaggcaggt acacgtcgac
     1201 tttcgacccc atcttgatca tgccgaaccg atcgccccgg acaagccggt cgcctttccg
     1261 gagcctgcac acaatgcggc gggcaaggaa tcccgctacc tggacgagca gcaccggtcc
     1321 gtaaccggtg gcaaggccca cgcacattct ctcgttaagc tccgaggcct tgggttcaaa
     1381 ggccatccat ttcctcccgg gaatgtactc catccattcc accgttccat cgcagggaac
     1441 acggttgacg tgaacgtcga gagggttcat gaaaatgccc accttgaccg cctttccggt
     1501 aaagggatgg tccgtttcca tcacctccac cactttgccg tcggcgggag agagaaagcc
     1561 gggcccgtcg gga
//
LOCUS       DAOTHD010000583         1571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_94078_length_1571_cov_2.908971, whole genome shotgun sequence.
ACCESSION   DAOTHD010000583 DAOTHD010000000
VERSION     DAOTHD010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94078_length_1571_cov_2.908971"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="P5219_09410"
     CDS             <1..388
                     /locus_tag="P5219_09410"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRX27019.1"
                     /translation="DGAPLPVSFNSNWSERHVAFVCGLGTFGLSKGLITSLGVAGRFG
                     SVITSFPLVPDNVVPEDLYGNCTLCGECAAKCPAGAISPEKGKDHVLCSEYLGLMKEK
                     HSPRYGCGKCQTGVPCEKGIPAKRHR"
     gene            complement(458..>1571)
                     /locus_tag="P5219_09415"
     CDS             complement(458..>1571)
                     /locus_tag="P5219_09415"
                     /inference="COORDINATES: protein motif:HMM:NF037527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27020.1"
                     /translation="AHSRELCVSLFMPAFRAGRETEQNPIRFKNLLKEAENRLSASGA
                     RPPEIQELFKPARSLVDDPLFWQHQGDGLAVFLSADYFKKYRLPLTFQESVTLARRFH
                     FTPLLPYFSGEGRFYVLALSRNMIRLLEGSRHSVDEVDVESLLPSLGDAFRFDRFEKQ
                     LQFHTGTAAGRSGGRAAMFHGHDPSDEEKGRLLRWFGKIDGDLPEILRGSRSPLVLAG
                     VEYLLHLYREANSYPFLLEEGIPGNPDELRPEELHARAFPIAEKALSRAAGQALEKYA
                     GLSGSESVSSNVEKILEGAHHGRVDVLFVDSGARMTGCFDPEENRVLTGESCRGEEED
                     LLNLAAVQTLVHGGTVYGVSSGSLPGGEPLGAVFRF"
BASE COUNT          316 a          497 c          456 g          302 t
ORIGIN      
        1 cgacggcgct cctctgccgg tatcgttcaa cagcaactgg tcggagcggc atgtggcctt
       61 cgtctgcggc ctcggcacct tcggcctctc caaagggctc attacttccc tgggtgtcgc
      121 cgggaggttc ggcagcgtga tcacttcttt cccgctggtt cccgacaacg tcgtcccgga
      181 ggatctgtac ggcaactgca cgctgtgcgg ggaatgcgcc gccaagtgtc ccgccggcgc
      241 catctccccg gaaaagggca aagatcacgt gctctgctcg gagtaccttg gcctcatgaa
      301 agaaaagcac tccccgcggt acggatgcgg gaagtgccag acaggagtgc cctgcgaaaa
      361 aggcattccc gcgaaacgcc acagataaag ccccgggccg cctgaacgtc aaaggggtgc
      421 agggacagtc cctgcacccc tttgactggt tgtctcttca gaaccggaag acagccccca
      481 gaggttcgcc tcccggaagt gatcccgaac tgacgccata gaccgttcct ccatggacaa
      541 gagtctggac agccgcaaga ttgagaaggt cctcctcttc tccccggcac gattccccgg
      601 tcagaaccct gttctcttcc gggtcgaaac atcccgtcat acgggctccg ctgtccacga
      661 aaagcacgtc caccctcccg tgatgggcac cctcaaggat tttttctacg tttgaagaaa
      721 cactctccga gcccgaaagg ccggcgtact tctccagagc ctggccggcc gcccgggaca
      781 gggctttttc cgcaatgggg aaagcccggg cgtgcagctc ttcgggacga agttcgtcgg
      841 gatttcccgg tataccctcc tcgagcagga aggggtagga attggcctcc ctgtagaggt
      901 ggagcaggta ttccactcct gccagaacca ggggggatct gctcccgcga agtatttccg
      961 ggagatcccc gtctattttc ccgaaccacc ggagcagccg ccccttctct tcatcactcg
     1021 ggtcgtggcc gtggaacatg gccgccctgc cccccgacct tcccgccgcc gttcccgtat
     1081 ggaactgaag ctgcttttca aaacggtcga aacggaaggc atctccgagg ctcggaagca
     1141 gggattccac gtccacctcg tccacggagt gcctggaacc ctccagcagc ctgatcatat
     1201 tcctgctcag ggcaagcacg tagaacctcc cttctccgga gaaataggga agcaggggtg
     1261 tgaagtgaaa gcggcgggca agagtgaccg attcctggaa tgtgagggga agcctgtact
     1321 ttttgaagta gtcggcagac aaaaaaaccg ccaggccgtc tccctggtgc tgccagaaca
     1381 ggggatcgtc cacaaggctc cgggcaggct tgaacagttc ctggatctcc gggggccgtg
     1441 ccccggaggc ggaaagcctg ttctccgcct ccttgagcag gtttttaaag cggatagggt
     1501 tctgctccgt ctcccttccg gcccggaatg cgggcatgaa aagggacacg cagagttccc
     1561 tcgaatgagc g
//
LOCUS       DAOTHD010000584         1570 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_94180_length_1570_cov_2.933333, whole genome shotgun sequence.
ACCESSION   DAOTHD010000584 DAOTHD010000000
VERSION     DAOTHD010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94180_length_1570_cov_2.933333"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="P5219_09420"
     CDS             complement(<1..622)
                     /locus_tag="P5219_09420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRX27021.1"
                     /translation="MVTSIRENLELVREEIRQAAARSGRREEDILLVAVTKTRPVEDV
                     VEAVRCGVTSVGENRVQELLEKRALWPDGDEPEWRLIGHLQRNKARKALSAASAVDSV
                     DSPELAAVLSRIASEEGRTLRVLIEVNTSGEASKHGVAPEEAENLLHGILENCPALRP
                     EGFMTIGPLAGGEKEVRKAFALLRTICETARDNTGLALPGPSMGMSG"
     gene            complement(616..>1570)
                     /locus_tag="P5219_09425"
     CDS             complement(616..>1570)
                     /locus_tag="P5219_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27022.1"
                     /translation="KRDGAGDVLKEFVSALFRRFLYFFLILVIAVGTVYIYFLWWERR
                     ESLNPEVVSAVPFTHTDRIPARAILLWREELVRSRWSGTVTFPFPGPRRVPRGETLAV
                     VDASSGKMAVRADRTGYFIPALDGMEGRWAYSAFWNGMAPLPSAPPAQFLGPGAFVEK
                     GRPVGKLVPQPQDLRCILYADLTPSLERDIKAGFVRVKTKETEWPAKAEVRVARFFES
                     KVKLYLTLPFFPASVITSREMDLLLEAGERSGVSVPESAVLLREGRLGVLLADGNMVH
                     FRAVRGVPVEGGRFFITEGLKPGQIVVLHAQSGKEGKIRLW"
BASE COUNT          303 a          542 c          448 g          277 t
ORIGIN      
        1 cgccgctcat acccatggac ggcccgggaa gagccagccc cgtgttgtcc cgcgccgttt
       61 cgcagatggt ccggagaagg gcgaaggcct tccgtacttc cttctctcct cccgccagag
      121 gcccgatggt catgaaacct tccgggcgga gggcgggaca gttctcgaga atgccgtgaa
      181 gcaggttttc cgcctcctcc ggagcaaccc cgtgcttgga ggcttcgccg gacgtgttca
      241 cctcgatgag cacccgcaga gtcctgccct cttccgaggc aatcctcgaa agaacggccg
      301 ccagctccgg tgagtccacc gaatccacgg cggaggcggc cgatagggct ttcctggcct
      361 tgttccgctg aagatggccg atgagccgcc actcaggctc gtccccatcg ggccagaggg
      421 cccgcttctc aaggagttcc tggaccctgt tctcaccgac ggacgtcacg ccgcagcgca
      481 ccgcttcgac gacatcctcc accgggcggg tcttcgtcac ggcaaccagg agaatatctt
      541 cttccctcct gccgctccgg gcggcggcct gccgaatttc ttcccggacc aactccaggt
      601 tctcccgaat agacgttacc ataaacggat tttcccttcc tttccgctct gggcatggag
      661 cactacgatc tgccccggct tcagcccctc tgtaatgaaa aaacgcccgc cttcaacggg
      721 aaccccccgg acggcccgga aatggaccat gttgccgtcg gccagaagca cacccagccg
      781 cccctcccga aggagcaccg ctgattcggg aacggagact ccgcttctct cccccgcttc
      841 gaggagaaga tccatctccc tggaggtgat cacggaggcg ggaaaaaacg ggagcgtcag
      901 gtagagtttc actttcgatt caaaaaaacg ggcaacccga acctcggctt tcgcgggcca
      961 ttccgtctcc ttcgtcttca cccggacgaa gcccgccttt atgtcccgtt ccagggacgg
     1021 cgtcaggtcc gcgtagagga tgcaccgcag atcctggggc tggggaacga gcttgcccac
     1081 aggccgccct ttctccacga aggctcccgg gccgaggaac tgcgctgggg gtgcggacgg
     1141 cagcggagcc atgccgttcc agaaggccga gtaggcccat cgtccctcca tgccgtcaag
     1201 agccgggata aaatatcccg tccggtcagc cctgaccgcc atcttccccg aggatgcgtc
     1261 caccaccgcg agggtctccc ccctggggac ccgcctcggt ccggggaaag ggaaggtcac
     1321 cgtgccgctc caccgggatc tcaccagttc ctcccgccag agaagaatgg cccgggcagg
     1381 tatcctgtcg gtgtgggtga agggaacggc ggatacaacc tccgggttca gggattcccg
     1441 gcgctcccac cacagaaagt agatgtacac cgtcccgacg gcgatgacaa ggatcaggaa
     1501 aaaatacaaa aacctgcgga acagcgctga gacaaattcc ttcaagacgt ctcccgctcc
     1561 gtcccgcttt
//
LOCUS       DAOTHD010000585         1570 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_94216_length_1570_cov_1.898350, whole genome shotgun sequence.
ACCESSION   DAOTHD010000585 DAOTHD010000000
VERSION     DAOTHD010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94216_length_1570_cov_1.898350"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="P5219_09430"
     CDS             <1..1040
                     /locus_tag="P5219_09430"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRX27023.1"
                     /translation="YSLMQVPVGILADTAGPRLTAAWGMAAAGAGALLFASAGSPFLL
                     FAGRILMGAGVAGAFVCTMKFLADNFEGERFATLSGITSLIGNAGGMTAQAPLALLVA
                     ALTWRWSFVLLGCVSLLLSLLCFLVLPRRGRRSFSLPMLQKGLRNVFSTGAMYSLSLN
                     YLASQACFLALSGTWGISYLRAVHRIDGAPLMTLLAAGVMAGAVAAGKLSDLWRSRKK
                     PLCLFALAHFFMWGLLVYLPGPFPRPGLAVLLGGLGFFAGALVIPWSVARELNFPEHT
                     GLSIAVMNTVAFLAVAVLTSSMGRVLDSAAAMPPADAWRHVLLLPLAVSAAGLVGALL
                     TPETFGRKKTG"
     gene            1205..>1570
                     /locus_tag="P5219_09435"
     CDS             1205..>1570
                     /locus_tag="P5219_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27024.1"
                     /translation="MILDVRTVFIITFLYGLLTGAGLVFASRSYSGTLRRSMNVMGAG
                     AVFLMSGVLAAGLQGIWLTSFSSVLLANFLMLWGAGEYYQALRLFDGQVTNRWFTFAV
                     AAAVTGVNLFFYAVVPSLST"
BASE COUNT          205 a          529 c          477 g          359 t
ORIGIN      
        1 tctattccct gatgcaggta cccgtgggca tcctcgctga caccgccggt ccgcggctca
       61 ccgccgcatg ggggatggcc gcggccgggg cgggagccct tctcttcgca tcggcgggaa
      121 gccctttcct cctctttgcc gggaggatcc tcatgggtgc cggcgtggct ggagccttcg
      181 tctgcaccat gaagttcctg gcggacaact tcgaggggga gcggttcgcc accctctcgg
      241 ggatcacgtc cctcatcggc aacgctggag gcatgacggc ccaggcgccc ctggccctgc
      301 tggtggccgc cctgacctgg agatggtcct ttgtcctgct cggctgcgtt tccctcctgc
      361 tctccctgct ttgcttcctg gtcctccccc ggcgcggaag acgttccttt tccctcccca
      421 tgctgcaaaa ggggctcagg aatgtgttct caacgggggc catgtattcc ctcagcctga
      481 actacctcgc cagccaggcc tgtttcctcg ccctctccgg cacgtggggg atctcctacc
      541 tgagggccgt tcaccggatc gacggggcgc cgctcatgac ccttctggcg gcaggcgtca
      601 tggcgggcgc cgtggccgcc ggaaagcttt ccgacctgtg gcggagcaga aagaagcctc
      661 tctgcctctt tgcccttgcc catttcttca tgtggggcct ccttgtctat cttcccggtc
      721 ccttcccccg gcccggcctc gccgtccttc tcggcggcct cggctttttc gccggagcgc
      781 tggtcatacc gtggtccgtg gccagggagc tcaatttccc ggaacatacc ggcctgtcca
      841 tcgccgtgat gaacaccgtc gccttcctcg cggtggcggt gctcacctcc tccatggggc
      901 gggttctcga cagtgcggcc gccatgcccc cggcagatgc ctggcggcac gtccttcttc
      961 tgcccctggc cgtttccgcg gccgggctgg tcggggcgct cctgacgccg gagaccttcg
     1021 ggcggaagaa aacggggtga ccggttcccc tcgctgagcg gcctgaccgg tacggcacgg
     1081 accttccgca ccccgggaaa gagatgatat aatccaaaag attcaccggt gcagagtatt
     1141 atgtgcataa tattccgggg agagccaaag agcgctcttc cttcccgaaa ggtcgttttg
     1201 cgccgtgatc cttgatgtcc ggacggtttt tatcatcaca ttcctttacg gtctgctgac
     1261 cggtgcgggg cttgtcttcg cgtcgaggtc ctactccgga accctccgga ggagcatgaa
     1321 cgtcatgggc gcgggcgcgg tgttcctgat gtcgggggtt ctcgccgcag gccttcaggg
     1381 gatatggctg acgtccttct cctcggtgct tctggccaat ttcctgatgc tctggggtgc
     1441 gggagagtat taccaggccc tccgcctctt cgacgggcag gtaaccaacc gttggttcac
     1501 ttttgcggtc gctgccgcag tgacgggggt gaacctgttt ttttacgccg tcgttccctc
     1561 cctgtccaca
//
LOCUS       DAOTHD010000586         1569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_94278_length_1569_cov_2.878468, whole genome shotgun sequence.
ACCESSION   DAOTHD010000586 DAOTHD010000000
VERSION     DAOTHD010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94278_length_1569_cov_2.878468"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="P5219_09440"
     CDS             <1..434
                     /locus_tag="P5219_09440"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRX27025.1"
                     /translation="GKHATGMEIYLMALPTDKDAMQLALIENREIAEGSNGESAKAAD
                     KKTRLLLSILGDMQQNAKINESTGFAEYLFKEGSQGGLKMRRVAQAPFFVLRGAAMPA
                     VLIETGFLTEKSEARMLNDTKYQQKMASSLAGGIADYLTRN"
     gene            474..1190
                     /locus_tag="P5219_09445"
     CDS             474..1190
                     /locus_tag="P5219_09445"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HRX27026.1"
                     /translation="MPRKYDDYDDGFEVRRREPEEEPFPLREERRPVRRRRTEEPETK
                     KAPLLIRIIAWLAVVAFCFVAGYVGTSFALRMLNKKDILMRKDVVSDRQEAKGVLEDG
                     SAEIRVNARKVAFTVYFPREGAIASEKTDILSGIMEDDIRHVFGKILSLVPGSFSPDM
                     KVLNVFRSGDTLFLNLNGPFISSLTKLGAKESTLFITTVVRTITENFPPLTKVRFLVN
                     GKVAGEGTPVDLTVPWQLPQ"
     gene            1187..>1569
                     /gene="rph"
                     /locus_tag="P5219_09450"
     CDS             1187..>1569
                     /gene="rph"
                     /locus_tag="P5219_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HRX27027.1"
                     /translation="MSRDSMVLRVDGRRNDELRPVTFQRNFTRYAEGAVLASFGETRV
                     LCTATVEEKVPSFLRGSGRGWVTAEYSMLPRSTSVRVSRSTSRGGPDGRSTEIQRLVG
                     RSLRACVDMESLGERTIILDCDVLQA"
BASE COUNT          358 a          446 c          453 g          312 t
ORIGIN      
        1 cgggcaaaca tgccacggga atggaaatct atctcatggc ccttcccacc gacaaggacg
       61 ccatgcagct cgcccttatc gaaaacaggg aaatcgccga gggcagcaat ggagagtcgg
      121 cgaaggctgc agacaaaaaa acgcggctgc tactgagcat cctcggcgat atgcagcaga
      181 acgcgaagat aaacgaaagc accggctttg ccgaatacct cttcaaggag ggatcccagg
      241 gcggcctcaa gatgcgccgg gtggcccagg ctcccttctt cgtccttcgt ggagccgcta
      301 tgcctgcggt gctcattgag accgggtttc tcacggaaaa atccgaagcc cggatgctga
      361 acgacacaaa ataccagcaa aaaatggcat cctcccttgc cggaggaatc gccgattacc
      421 ttacacgcaa ctgaccctga cgggaaccct gaccgaggga ggagaatgca gccatgcccc
      481 ggaaatacga tgactatgac gatggttttg aagtgagacg gagggaaccg gaagaggaac
      541 ccttcccctt gcgggaagaa aggcggccgg tgagaaggag gaggaccgag gagccggaaa
      601 cgaaaaaggc tcccctcctt atacgtatca ttgcctggct ggccgtcgtg gccttttgtt
      661 tcgtggccgg ctatgtcgga acgtctttcg ccctgcggat gctcaacaaa aaagatattc
      721 tcatgcggaa ggacgtggtc tccgaccggc aggaagccaa gggagtcctc gaggacggaa
      781 gcgctgaaat ccgggtcaat gcacggaaag tagcctttac ggtgtatttc cccagggaag
      841 gtgccatcgc ttcggaaaaa acggacatcc tctcgggtat catggaggat gacattcgcc
      901 atgtgttcgg caagattctc tcccttgttc cgggcagttt ttcccccgac atgaaagtgc
      961 tcaatgtctt ccggagcggc gataccctct ttctcaacct aaacgggcct ttcatctcct
     1021 ctcttacgaa actcggtgca aaggagagta ccctgttcat aaccaccgtg gtgaggacca
     1081 taacggaaaa ctttccccct ctaaccaagg tgcgtttcct ggtcaacggc aaggttgccg
     1141 gggaagggac ccccgtggac cttacggttc catggcagct cccccaatga gccgggattc
     1201 catggtgctg cgggttgacg gacggcggaa cgatgaactg cgtcccgtca ccttccagag
     1261 gaactttacc cgttatgcgg agggggctgt cctggcgtct ttcggcgaaa cccgggttct
     1321 ttgcaccgcc actgtggagg agaaagttcc ttcctttctc cggggatcgg gaaggggctg
     1381 ggtaaccgcc gagtattcca tgcttccgag gtccacgtcc gtccgggttt cccgctcgac
     1441 ttccaggggc ggacccgacg gaagaagcac cgagatccag cgccttgtcg gccgttctct
     1501 gcgggcctgc gttgatatgg aatcccttgg tgaaagaacc atcattctcg actgcgacgt
     1561 cctccaggc
//
LOCUS       DAOTHD010000587         1567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_94408_length_1567_cov_3.829365, whole genome shotgun sequence.
ACCESSION   DAOTHD010000587 DAOTHD010000000
VERSION     DAOTHD010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94408_length_1567_cov_3.829365"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="P5219_09455"
     CDS             <1..271
                     /locus_tag="P5219_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27028.1"
                     /translation="HIAELSRSQASASEEMAGAIDQVSHATIDVVQRVDAIRSASGET
                     AAASEGVAREASAMADRAEHMRNLLSRFILSKDTEKEEAGLVPAE"
     gene            complement(312..1070)
                     /locus_tag="P5219_09460"
     CDS             complement(312..1070)
                     /locus_tag="P5219_09460"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRX27029.1"
                     /translation="MNETRKDLIRRLTDLVSQGNVVTGGKLPPERELAALLGTTRPLL
                     REGLIALEALGLLDIRDRQGIFLAEGNPEEIKRVLGQAQIWPMEVLSQVMEIRQLLDP
                     GAAALAALRRKDRDLEKMDECISMLEKIHTEQDPHEAPLGAYWNTVLHATIFRATGNT
                     LLSRLYESLLEMSEKGISAMRMEVLDSAAPDRTEKILDQHRTLVAAIRDRDLLRAREA
                     SRLHLKFTIDTLVELSRVTPLSNFFAQRMDSVLK"
     gene            1246..>1567
                     /locus_tag="P5219_09465"
     CDS             1246..>1567
                     /locus_tag="P5219_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRX27030.1"
                     /translation="MVQEYDVLVIGGGVVGCAIARELSKFRVRTAVVEKEMDVGLGTS
                     CRNSGVLHAGFNYAPGTLRAILDVKGNQMMDDLCRDLKVKIKRIGKLTVALDDEDEAT
                     LRRLK"
BASE COUNT          298 a          430 c          513 g          326 t
ORIGIN      
        1 ccacatcgcg gagctctcca ggtcccaggc ttcggccagc gaagaaatgg ccggggccat
       61 agaccaggtc tcccatgcca ccatcgacgt ggtccagagg gtggacgcca tccggtccgc
      121 ctcaggggag acggcggcgg cgtcggaggg ggtcgcccgg gaggcgtcgg ccatggcaga
      181 cagggccgag cacatgcgga atctcctgtc ccggttcatc ctgtcgaaag acacggagaa
      241 ggaagaggcg gggctcgtcc cggccgagta aactgatggg gcggaccggt ttttttccgg
      301 tccgccgttt tttatttcag gacggaatcc atcctctggg cgaagaagtt ggagagagga
      361 gtaaccctgc tgagttccac gagggtgtcg atggtgaact tcaggtggag cctggatgcc
      421 tccctggcgc ggaggagatc ccggtcccgg atggccgcca cgagggtcct gtgctggtcg
      481 agaattttct ccgtccggtc cggtgccgcc gagtcgagga cttccatcct catggcggag
      541 attccctttt cggacatttc gaggagggac tcatagagcc ttgaaagaag ggtgttcccc
      601 gtggccctga aaatggtggc gtggaggacg gtgttccagt aggcgccgag aggcgcttcg
      661 tgaggatcct gctctgtgtg gattttttcg agcatggaga tgcattcgtc cattttctca
      721 aggtcccggt ccttccgccg cagggcggcc agcgccgcgg cgcccgggtc caggagctgc
      781 cgtatctcca tgacctggga gagcacttcc atgggccata tttgggcctg gccgagaact
      841 ctcttgattt cctccgggtt cccctcggcg aggaagatgc cctgcctgtc cctgatatcc
      901 agcaggccga gggcttcaag ggcgataagg ccttccctca gaaggggcct ggtggtgccg
      961 agcagagcgg caagctcccg ttccggaggc agcttgcccc cggtcaccac gttcccctgt
     1021 gacacaaggt ccgtcaagcg cctgatcagg tcctttcgcg tttcgttcat gggggcctcc
     1081 caacggttca gaatggtttt cgggcctgac aaaaagttaa aaaaagaact tgaaattttc
     1141 tgttgattcc ccttgtctct cttcctgcca ccatgataat ataaaatcgg tttgattgga
     1201 ataccttggt cgaccagatg ttttcccgaa agcgggtgtt acagggtggt tcaggagtac
     1261 gacgttcttg tcattggagg aggagtggta ggctgcgcca tcgccaggga gctttcgaag
     1321 ttccgggtga ggacggccgt cgtcgagaag gagatggacg taggcctggg gacgagctgc
     1381 agaaacagcg gcgtgctcca cgctgggttc aactacgctc ccggaacgct ccgggcgatt
     1441 ctcgacgtga agggcaatca aatgatggac gatctctgcc gggacctcaa ggtgaagatc
     1501 aagcgtatcg gcaagctgac ggtggcgctg gacgacgagg atgaagccac cctccgcagg
     1561 ctgaagg
//
LOCUS       DAOTHD010000588         1565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_94614_length_1565_cov_2.772848, whole genome shotgun sequence.
ACCESSION   DAOTHD010000588 DAOTHD010000000
VERSION     DAOTHD010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94614_length_1565_cov_2.772848"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="P5219_09470"
     CDS             <1..589
                     /locus_tag="P5219_09470"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HRX27031.1"
                     /translation="FRAVLPSLPFPKLSGERGGFITVSAEANFLRPLNAGVLTVSDKG
                     SRGEREDTSGPALAERLRGIGCDTVASSVVPDEHEAIVTTLQDWTDRHDLHLILCTGG
                     TGFSPRDITPEALEAIAERKVPGIGEAMRQASLKITPKAMLSRGNAVIRGETLIMSLP
                     GSARAATECFDAIAPALRHGVEILRGWDGECGSPS"
     gene            638..1525
                     /gene="rapZ"
                     /locus_tag="P5219_09475"
     CDS             638..1525
                     /gene="rapZ"
                     /locus_tag="P5219_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HRX27032.1"
                     /translation="MDSEFQGVKKCVILTGMSGAGKSTALKVLEDQGMFAIDNIPPAL
                     LSQLMVILGKHRAAVRNGVAAVVDVRGDTLLDDFPTAVSALKTQIPEVSILFLDASDR
                     ALLARFEATRRRHPLHYEGTFLDGISRERIRLRPILDLSDTVLDTSGLSLPQIRKKLL
                     SEVSGPALQDVLIFTSFGFKYGPPADCDFLFDVRFLSNPYYIPELQDLSGQDGEVQGH
                     IWNSGEAEEFWKRLVSFLEHVIPLYFSTGKSHLHIGIGCTGGRHRSVAVAERLAAYFR
                     PFGGMCSLRHRDIAREQGW"
BASE COUNT          344 a          447 c          442 g          332 t
ORIGIN      
        1 ttttcgggct gttctgccgt cccttccctt tccgaaactc agcggggaga gagggggatt
       61 catcactgta tccgcagaag cgaatttttt acgtcctctc aatgcagggg tactcactgt
      121 cagcgacaag ggaagcaggg gagaaagaga agacacttca ggccccgccc tggccgaacg
      181 gctgagggga atcggctgcg ataccgtcgc ctcttccgtc gtccccgacg aacatgaagc
      241 gatcgtgacg acactgcagg actggacaga ccgacacgac ctccacctga tactgtgcac
      301 cggaggaacg gggttctccc cgagggacat cacacctgaa gccctggagg ctattgcgga
      361 aagaaaagtc cccggaatcg gcgaggcaat gcgtcaggca tccctgaaaa taacgcccaa
      421 agccatgctg agcaggggca atgccgtcat ccggggtgaa actcttataa tgtccctccc
      481 tggaagcgcc agggccgcga cggaatgttt cgatgccata gccccggcac tgaggcatgg
      541 tgtagagata ctccgcggat gggacgggga gtgcggctct ccttcataag aaagcgaacc
      601 ttcaggtttc cggcagaagg ggaacggggg tagtttcatg gacagtgaat tccagggcgt
      661 gaaaaaatgc gtcattctca ccggcatgtc cggagcaggc aaatccactg ctctgaaggt
      721 gcttgaagat cagggaatgt tcgccatcga caatatccct ccggcgttgc tttcccagct
      781 tatggtcatc ctcggaaaac accgggccgc cgtgaggaac ggggtagcag ctgtagttga
      841 cgtccgtgga gacactcttc tcgacgattt tccgacggcg gtctcggcat tgaagaccca
      901 gattcccgag gtcagcattc tctttctcga cgcctcggac agagcgttgc ttgcccgttt
      961 cgaagcgacc cgcagacggc atcctctcca ctacgaaggc acctttctcg acgggatctc
     1021 cagggaacgg attcgcctaa ggccgattct tgacctttcc gacacggtac tcgacacgtc
     1081 cggcctctct cttccgcaga taaggaagaa acttctttcc gaagtgagtg gtcctgccct
     1141 gcaggatgtt ctcatcttta cctctttcgg tttcaagtac ggacctcctg cggactgcga
     1201 cttccttttc gacgtccggt tcctctcaaa cccatactat ataccggaac tccaggatct
     1261 ctcaggacag gacggagagg tccaaggcca tatctggaat tcaggggaag ctgaggagtt
     1321 ctggaaacga ctggtttcct ttctcgagca cgtcattccg ctctatttca gcacgggaaa
     1381 atcccacctt cacatcggca tcggctgtac gggaggccgc cacagatcgg tggccgtggc
     1441 cgaaaggctg gcggcgtatt tccgaccttt cgggggcatg tgctctctca ggcacaggga
     1501 tatcgcgaga gaacagggct ggtgagcatg gacagcatcc tggcctacat cctcggttta
     1561 atgac
//
LOCUS       DAOTHD010000589         1565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_94638_length_1565_cov_2.201325, whole genome shotgun sequence.
ACCESSION   DAOTHD010000589 DAOTHD010000000
VERSION     DAOTHD010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94638_length_1565_cov_2.201325"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="P5219_09480"
     CDS             complement(<1..563)
                     /locus_tag="P5219_09480"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27033.1"
                     /translation="MKKTLALVLTVVFLFAGAQGALAQDVKWRLVTHAMPGTEQQRIA
                     EVFCETVKTLSGGKFVIEPYAAGVLFPVFESFDGVANGVVDAAMVYSAYWTGKDPLFT
                     LTTQPGSPLGTFAEGAYLVEKLEPWFEKLYAKHRIKYLGHAMTSPINEQLMSVTPINS
                     IEDVKGKKIRSSGFGALFYRALGATTVS"
     gene            complement(768..>1565)
                     /locus_tag="P5219_09485"
     CDS             complement(768..>1565)
                     /locus_tag="P5219_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRX27034.1"
                     /translation="GLFAGGLSAGIFLAVLYIGYVLIRGLLDPDFCPSLPRDERATWK
                     EKLISMKSVFFPGLLIMVVLGSILLGVATPTEAAAFGAAGALLIGLLKGRLTWEIVYT
                     SCFETLGVSAMVGWTMIGAGAFGSVFSGLGGNTLITNIAMNMPGGPNMVFLVSALFIF
                     ILGMFLEPGAIIFLAVPIIAPILSKLGFDPLWVGLAFNVLLQTAYLSPPFGFSLFYLK
                     GCTPPDVDIVEIYKASVPFMLLQVVCITSIFMFPDIVMWLPRYIMGM"
BASE COUNT          343 a          442 c          484 g          296 t
ORIGIN      
        1 gacaccgtgg tggctccaag agccctgtag aaaagggcgc cgaaaccgga ggagcggatc
       61 ttctttccct tcacatcctc gatggagttg atgggcgtga cggacatgag ctgctcgttg
      121 atggggctgg tcatggcatg gccgagatac ttgatgcggt gcttggcata gagcttctcg
      181 aaccagggct cgagcttctc caccaggtag gcgccctcgg caaaagtacc gagaggggaa
      241 ccgggctggg tggtgagggt gaagagggga tccttgcccg tccagtaggc gctgtagacc
      301 atggcggcat ccaccactcc gttggccacg ccgtcaaagg actcgaacac ggggaaaagc
      361 acgccggccg catagggctc gataacgaac ttgccgccgg acagggtctt taccgtctcg
      421 cagaacactt cagctatgcg ctgctgctcc gttcctggca tagcatgggt caccaggcgc
      481 cacttcacgt cctgggcaag ggccccctgg gctccggcaa aaagaaatac aacggtcagt
      541 acaagtgcaa gggtcttttt cattgggtca aatttcctcc ttttctgggt ttgggtgtcg
      601 cttttcatga acactccggt gcgcctttgc ttcccatact ctttgactgt tttcctgctt
      661 tcctggcggc gccgttcgca ctctcccgaa tagctccccc cctttcgctc cggtttttcc
      721 tgtttcagag ttcaaggttc acaaggggct tcggtacctg ccgcccccta catgcccatg
      781 atgtatctcg gaagccacat cacgatgtcg gggaacatga aaatggacgt gatgcagacg
      841 acctgcagca gcatgaaggg aacgctcgcc ttgtaaattt ccacgatgtc cacgtcggga
      901 ggagtgcatc ccttcaggta gaagaggctg aaaccgaaag gaggcgacag gtaggcggtc
      961 tgcagcagca cgttgaacgc gagacccacc cacagggggt cgaagccgag ttttgaaaga
     1021 atgggggcaa tgatcggaac ggcaagaaag atgatggctc cgggttccag gaacatgccg
     1081 aggatgaaga taaacagggc ggacaccagg aagaccatgt tgggtccccc gggcatgttc
     1141 atggcgatgt tggtgatgag ggtgttgccc ccgaggccgg agaaaacgga tccgaaggcg
     1201 cctgcgccga tcatggtcca ccccaccatg gcggacaccc caagggtctc gaagcaggag
     1261 gtatagacaa tctcccaggt gaggcgcccc ttcagcaggc cgatcaggag ggctcccgcc
     1321 gctccgaagg ccgccgcctc cgtgggggtg gccactccca aaaggatgga gccgaggacc
     1381 accatgatga gcagccccgg gaagaaaacg ctcttcatgg aaatcagctt ttccttccac
     1441 gtggcccgtt cgtcccgagg cagggacggg cagaaatccg ggtcgagaag tcccctgatg
     1501 aggacgtacc cgatgtacag tacggcgagg aagataccgg cggaaaggcc gccggcgaaa
     1561 aggcc
//
LOCUS       DAOTHD010000590         1553 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_95654_length_1553_cov_2.714286, whole genome shotgun sequence.
ACCESSION   DAOTHD010000590 DAOTHD010000000
VERSION     DAOTHD010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95654_length_1553_cov_2.714286"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="P5219_09490"
     CDS             complement(<1..401)
                     /locus_tag="P5219_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HRX27035.1"
                     /translation="MKIAEPTVERLVQYHRLLEQLVAEGQKVVSSQEMGEMLSFKASQ
                     VRKDLSYFGEIGKRGVGYHVDRLHKHISRILASPRKWSIGLVGVGKLGEALLGYKAFQ
                     SEKFRIEALFDVDTDKIGKDIQGIPCYSSDA"
     gene            complement(457..837)
                     /locus_tag="P5219_09495"
     CDS             complement(457..837)
                     /locus_tag="P5219_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRX27036.1"
                     /translation="MKKIISTTAAPAAVGPYSQAVRAGNFLFCSGQIPIDPATGNFVE
                     GGVEQQAEQVLKNVRAVLEGAGYSLSDVIKATVFAVSMGDFAVVNGVYARYFDKEPPA
                     RSFVEVSALPKGALVEIEVVAWKE"
     gene            complement(858..>1553)
                     /locus_tag="P5219_09500"
     CDS             complement(858..>1553)
                     /locus_tag="P5219_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRX27037.1"
                     /translation="YSTAIGDEGGFAPDLKSNREAIDLILEAVEKAGYAPGKDVSLAL
                     DPAASEFFKDGKYHFTGENKVFSAEEMVDYWEELCRDYPILSIEDGMAEDDWAGWSVM
                     TSRLGKKIQIVGDDLFVTNPEKLTRGIEEGVANAVLIKLNQIGTLSETLNVIALAKRY
                     GYGTIISHRSGETDDSFISDLAVATGAGQIKTGAPARIDRVAKYNQLLRIAEDLERAP
                     YARLSQFRGNPYR"
BASE COUNT          282 a          475 c          449 g          347 t
ORIGIN      
        1 atggcatccg aggaatagca cggaatgccc tgtatgtcct tgccgatttt gtccgtgtcc
       61 acgtcgaaca gagcttcgat gcggaatttt tcgctctgga acgccttgta tcccagcagc
      121 gcttctccga gctttcccac tccgacgagc ccgatggacc atttcctcgg cgaagccagt
      181 attcggctta tatgcttatg gagacggtcc acgtggtatc cgacgcctct cttgccgatc
      241 tccccgaaat aggaaagatc ttttctcacc tggctggcct tgaaagaaag catctccccc
      301 atttcctggg aggaaaccac tttctggcct tcggcgacga gctgctccag caggcggtgg
      361 tactgtacaa gccgttcaac ggtgggttca gcaatcttca tttccgcact tcctccagtg
      421 gagtgaaggg gcggagaaac gtctccgccc cgggagctat tctttccagg cgacgacttc
      481 gatttccacg agggcgccct tggggagggc gcttacttcc acgaaggatc tcgcaggcgg
      541 ctccttgtcg aagtaccgcg cgtacactcc gttgacaacg gcgaaatcgc ccatgctcac
      601 agcgaaaacg gtggccttga tcacgtcgga aagggagtag cccgcccctt cgagaaccgc
      661 cctgacgttc ttcagaacct gctccgcctg ctgctccact ccgccctcga cgaaattccc
      721 cgtggcagga tcgatgggaa tctgtcccga gcagaaaagg aaattccctg cccgaacggc
      781 ctggctgtaa ggtccgacgg ctgccggggc cgccgtggtg gaaattatct tcttcatcgt
      841 gtgtcctcct tgtgatgtta tctgtacggg ttgccccgga actgggaaag ccgggcatag
      901 ggtgcccgct ccaggtcttc ggcgatgcga agaagctggt tgtacttggc gaccctgtcg
      961 atccgcgccg gagctcccgt cttgatctgg cccgctcccg tggcgacggc caggtcgctg
     1021 ataaaggagt cgtccgtctc gcccgagcgg tgggagatga tggtcccgta gccgtaccgc
     1081 ttcgcgaggg cgatgacgtt aagggtctcg gagagggtac cgatctggtt gagcttgatg
     1141 agaaccgcgt tcgcgacccc ttcctcaatg cccctggtga gcttttccgg gttggtgacg
     1201 aacaggtcgt ccccaacgat ctggattttc ttcccgagtc ttgaggtcat gaccgaccag
     1261 cctgcccagt cgtcctccgc cattccgtcc tcgatagaaa ggatgggata gtcacggcag
     1321 agctcctccc agtagtccac catctcctcg gccgaaaaga ccttgttctc gccggtgaaa
     1381 tgatacttgc cgtccttgaa gaactcggag gcggcagggt cgagagccag gctcacgtcc
     1441 ttgccggggg cgtatccggc cttctccacc gcctcgagaa tgaggtcgat ggcctcgcgg
     1501 ttgctcttca ggtcgggggc gaaaccgccc tcgtcaccta tggcggtgct gta
//
LOCUS       DAOTHD010000591         1551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_95794_length_1551_cov_3.650401, whole genome shotgun sequence.
ACCESSION   DAOTHD010000591 DAOTHD010000000
VERSION     DAOTHD010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95794_length_1551_cov_3.650401"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="P5219_09505"
     CDS             <1..1095
                     /locus_tag="P5219_09505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX27038.1"
                     /translation="HEIRNPLNVLAATFDRLAGTCGDEGAAEGLRVIGTAKDHILQIL
                     NDILDLSKIEAGWMDVSVSPFSPGELAGECAALFSPQAELKGLTFSTGVSPDVPEHAL
                     GDPLRIRQILFNLLGNAVKFTSRGGVAFSVDAAPDLSGSPRLLFSVSDTGPGIPPEQE
                     ESLFEPYSRGTRPSPGDPGGTGLGLSISRRIARLLGGELTAGSTPGKGAVFTLSLPLI
                     TGGPGGEDVPKGKEEIPPVPERLTILAADDHPVNRALLSKMLSLPGWQVILASNGKEA
                     LELAKREIPDVVLLDLEMPVLDGLEAARAIRNLTPVDDPRPLLLGLTGHDRDRALPLC
                     REAGMDGCFTKPFSAGDILGEIARRFGKSC"
     gene            1211..>1551
                     /locus_tag="P5219_09510"
     CDS             1211..>1551
                     /locus_tag="P5219_09510"
                     /inference="COORDINATES: protein motif:HMM:NF013935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HRX27039.1"
                     /translation="MSPKIAPTRGNMVKFASSLKLAEKGHDLLEQKRTILLMELMGKI
                     REAKEIQRSLEDVFSEAYFSLQMAGLSLGIENVEELSYSVPETDGFTVRLHSVMGVEI
                     PKVDPVNRPPEP"
BASE COUNT          288 a          521 c          456 g          286 t
ORIGIN      
        1 cacgagatcc gcaaccccct gaacgtgctg gcagccacct tcgaccgcct ggccggaacc
       61 tgcggtgatg aaggagccgc agagggactc cgcgtcatag gcacggcgaa ggaccatatt
      121 ctccagatcc tgaacgatat cctggacctc tcgaagatcg aggcgggatg gatggacgtt
      181 tcggtgtccc ccttttcgcc cggggagctg gccggagaat gcgcggctct cttctccccc
      241 caggcggaac tgaaggggct gacgttcagc accggggtga gcccggacgt tcctgaacac
      301 gccctcggag accccctccg catccggcag atcctgttca acctcctggg aaatgcggtc
      361 aagttcacct cccggggcgg cgttgcattt tccgtggatg cggccccgga cctttccggt
      421 tcgccccggc tgctcttctc ggtctcggac acgggaccgg gaatcccacc ggagcaggaa
      481 gaaagccttt ttgaacccta ctcccgggga acccgtccct ctccgggaga ccccggcgga
      541 acggggctcg gcctttccat cagcaggcgc atcgcccggc tcctgggcgg agagctgacc
      601 gccggcagca ctccgggaaa gggggcagtc ttcaccctgt cgcttcccct catcaccggc
      661 ggtcccggcg gagaagatgt cccaaaaggg aaggaagaga tcccgcccgt gccggaacgg
      721 ctgaccatcc ttgccgccga cgaccacccg gtgaaccggg ccctcctgtc gaaaatgctc
      781 tctctccccg gctggcaggt cattcttgcg tcaaacggaa aagaagccct ggagctggcg
      841 aaaagggaga ttcccgacgt ggtccttctc gacctggaaa tgcccgtcct ggacggactg
      901 gaagccgccc gggccatcag gaacctgacg ccggtggatg atccgcgccc cctccttctc
      961 ggcctcacgg gccatgatcg ggacagggcc cttcccctct gccgggaggc cggcatggac
     1021 ggatgcttca ccaagccttt ttccgccggg gacatcctcg gcgagatcgc ccggaggttc
     1081 ggaaaaagct gctgagctgt cttccccgag ctttcctttt cctgtctttt tttcctcccc
     1141 tctccacgtc cgccgcattt gacattttcc tggaacaggt ctattctccc ctctacggag
     1201 gaatcggatc atgagcccga aaatcgcccc cacccgggga aacatggtga agttcgcatc
     1261 atcgctgaaa ctggccgaga agggacacga ccttctcgag cagaaacgga ccatcctcct
     1321 tatggagctg atgggaaaga tcagggaggc caaggaaatc cagcgctccc tggaggacgt
     1381 cttctccgag gcctacttca gcctccagat ggccgggctt tcccttggca tcgagaacgt
     1441 ggaggagctc tcctattctg tccccgaaac ggacggattc accgtgaggc ttcactccgt
     1501 catgggcgtg gagatcccga aggtggaccc cgtgaacagg ccgcccgagc c
//
LOCUS       DAOTHD010000592         1550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_95920_length_1550_cov_2.936455, whole genome shotgun sequence.
ACCESSION   DAOTHD010000592 DAOTHD010000000
VERSION     DAOTHD010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95920_length_1550_cov_2.936455"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="P5219_09515"
     CDS             complement(<1..472)
                     /locus_tag="P5219_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27040.1"
                     /translation="MREEDRIWGAPCPGADVQQEKEMTMVKQRFLKLSVSFFVLFVLM
                     LSPLSEQTGFAQGTQTEAAEKAPFMYSLAATWEGKGIVRYRIAVQPAGDTVLEGLKIV
                     AAVPAKGKVSVVTAGAAAAEDKVALSVDKTTPGKVPMLEYTVLTDAAGPDAVKPV"
     gene            901..>1550
                     /locus_tag="P5219_09520"
     CDS             901..>1550
                     /locus_tag="P5219_09520"
                     /inference="COORDINATES: protein motif:HMM:NF014754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HRX27041.1"
                     /translation="MKIRCIDGERYRNAFLAGTAALERRREHLNSINVFPVPDGDTGD
                     NMVFTCRSIAEGTRVSRSLAETASSMAEAAFSGARGNSGIIFCQYIHGLARAFGPYPR
                     VDAPAFAESLLSAVPFAEEALHRPVEGTILTVMREWSHAVRRISETETDFAVLLPLSL
                     EDARKSLRETPARMDLLAREKVVDAGAQGFVDFLEGVVEFLGRGSLRHFSLRSATAA"
BASE COUNT          317 a          443 c          469 g          321 t
ORIGIN      
        1 aaacgggttt cacggcatcg ggcccggcgg cgtcggtgag gacggtgtat tccagcatgg
       61 gaaccttgcc cggggtcgtc ttgtccaccg agagggccac cttgtcctcc gctgcggcgg
      121 ctccggcggt caccacggac acttttccct tcgccggtac ggccgcgacg attttcaggc
      181 cttcaagaac ggtgtccccg gcgggctgaa cggcgatgcg gtaccggaca attcccttgc
      241 cttcccaggt ggccgccagg gaatacatga agggggcttt ttcggctgcc tcagtttgcg
      301 taccctgggc aaatcctgtc tgctcggaca ggggcgaaag catcaggacg aaaagaacga
      361 agaatgaaac gctgagcttc agaaatcgct gcttcaccat ggtcatctcc ttttcctgct
      421 gtacgtcggc gcccgggcag ggcgctcccc aaatacggtc ttcctcgcgc atggagccgg
      481 aattttccgc agacctcctt tccccggcgt tggaacaaag ggctgtagtg tcgacgtgtg
      541 atacaagcaa tagttggcaa tgactaatta tatcgggcac tgaataataa atatcatgca
      601 cggtgtattt tgtcaaaaaa tggtgtccca ttttctgttg taattcagaa gacaagagag
      661 gatggccggg tcagtttatg ctgtcgctgc agggatattc tccttgcggc ggtggtttgc
      721 aaaatgtcac tcccaggtga cataggcgga gaaaaaaact ataatgcaaa aaagcagcaa
      781 tacaatccgc tgcagagtat caattccttg ccgtcaatac ccggtccttc cgtgagaggg
      841 cgacatcgtc cgctgtcctt ttcgggaaga ctgacaacgg aggttgttca ccccttgaag
      901 atgaaaattc gatgtatcga cggagagaga tacaggaacg ccttcctggc gggaaccgcc
      961 gccctcgagc gccggaggga gcacctcaac agcatcaacg tctttccggt ccctgacggg
     1021 gacacggggg acaacatggt cttcacctgc cgctccatcg ccgaagggac acgggtttcc
     1081 aggtctctgg cggagacggc ctcctccatg gcggaggccg ccttttcggg ggccagggga
     1141 aactcgggga tcatcttctg ccagtacatc catggactcg ccagggcgtt cgggccctat
     1201 ccgagggtgg acgccccggc attcgccgag agcctgctct ccgccgttcc cttcgccgag
     1261 gaagcgctcc acaggccggt ggagggcacc atcctgacgg tcatgaggga gtggtcccac
     1321 gcagtccgca ggatcagcga aacagagacg gacttcgccg ttctgcttcc cctgtcgctg
     1381 gaggatgcca ggaaatcgct gcgggaaact ccggccagaa tggacctcct ggcccgggag
     1441 aaggtggtgg atgccggcgc ccagggcttc gtcgactttc tggaaggggt agtggaattc
     1501 ctggggcgtg gaagcctgag gcatttttcc ctgcgttcag ccaccgccgc
//
LOCUS       DAOTHD010000593         1549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_96003_length_1549_cov_3.291834, whole genome shotgun sequence.
ACCESSION   DAOTHD010000593 DAOTHD010000000
VERSION     DAOTHD010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96003_length_1549_cov_3.291834"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /gene="sucC"
                     /locus_tag="P5219_09525"
     CDS             complement(<1..207)
                     /gene="sucC"
                     /locus_tag="P5219_09525"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF020031.2"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HRX27042.1"
                     /translation="MKLYEFQGKALFREAGIPVPRGAVVTASDRSGLFAPSAVKAQVL
                     SGGRGKAGGVLLVSTQAEAEKAAGS"
     gene            complement(204..761)
                     /locus_tag="P5219_09530"
     CDS             complement(204..761)
                     /locus_tag="P5219_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRX27043.1"
                     /translation="MNTKQTSIRFCGFGGQGIILSSVILGEAAVTKEGIYAAQSQSYG
                     SEARGGQCQSEVVLSKNPIGTPTNEQNDILVAMFQAAYMSHKHELKKGGVLFVDSGLV
                     PDLGDVDPSVTVWKVPATETAIRLGSKMAGNMVMLGFIQEKTGLVSRDSLLAAIRESV
                     KEKYVEMNTAAFEEGIRMAKEDTGA"
     gene            complement(761..>1549)
                     /locus_tag="P5219_09535"
     CDS             complement(761..>1549)
                     /locus_tag="P5219_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRX27044.1"
                     /translation="HFFCPGCGCGQVVSAFLRAVEALGLDLGSMVGIGGVGCTARIPV
                     YINMDCLHGVHGRTLAWATGIKLHKPDTKVVVFAGDGDALSIGGNHFIHAARRNLDVT
                     FVVVNNFNFAITGGQVAPMTPDGSVTMTTPYGSSEPPFDMCKLAVTAGATYVARTLTS
                     NPAQMTTFLKNALNHKGFSVVEILSQCPTHFGRYALGSGSPKGLVEWIKSHTVPASAA
                     EKMSPEETAGKYLVGEFVNVEKPVFRGSTVYTPSTCGCGGKEAK"
BASE COUNT          259 a          481 c          513 g          296 t
ORIGIN      
        1 ggagccggcg gccttttcag cttccgcctg ggtggagacg agcagaacgc cgcccgcctt
       61 tccccgcccc ccggagagaa cctgggcctt caccgcagag ggggcgaaga gacccgaccg
      121 gtcggaggcg gtgaccacgg cgccccgggg taccggtatg cccgcttcgc ggaacagagc
      181 cttgccctgg aattcgtaga gcttcatgcc cccgtgtcct ctttcgccat gcggatgccc
      241 tcttcaaagg cggcggtatt catctccacg tacttctcct tcaccgactc ccggatggca
      301 gccaggagac tgtccctgga gacgaggccc gttttttcct ggatgaagcc cagcatgacc
      361 atgttgcccg ccatcttgct gccgaggcgg atggccgtct ctgtggcggg aaccttccac
      421 acggtgacgg agggatccac gtcgccgagg tcgggtacga ggccgctgtc cacgaaaagg
      481 acgccgccct tcttcagttc atgtttgtgg ctcatgtagg ccgcctggaa catggccacg
      541 aggatgtcgt tctgctcgtt ggtgggggtg ccgatggggt tcttcgacag gaccacctcg
      601 gactggcact ggccgccccg ggcctcggag ccgtaggact gggactgggc ggcatagatg
      661 ccttccttgg tcaccgccgc ctcgccgaga atgacggagg acaggatgat cccctggccg
      721 ccgaagccgc agaaccggat gctcgtctgt ttcgtgttca ttacttggcc tccttcccgc
      781 cgcagccgca ggtcgacggg gtgtacacgg tgcttccccg gaagacgggt ttttcaacgt
      841 tgacaaactc gcccacaagg tacttccctg ccgtttcctc gggagacatt ttttcggcgg
      901 cggacgccgg aacggtatgg ctcttgatcc actccaccag gcccttgggg ctgccgcttc
      961 cgagggcgta ccggccgaaa tgggtcgggc actgggacag aatttcaacc acggagaatc
     1021 ccttgtggtt cagggcattc ttcaggaagg tggtcatctg ggcgggattg gaggtcaggg
     1081 tcctggccac gtaggtcgca cccgccgtta ccgccagctt gcacatatca aagggaggct
     1141 cgctgcttcc gtaaggggtg gtcatggtga ccgagccgtc gggagtcatg ggcgccacct
     1201 ggccgccggt tatggcgaag ttgaagttgt tcaccaccac gaaggtcacg tcgaggttgc
     1261 gccgggcggc gtggatgaaa tggtttccgc cgatggagag ggcgtcgccg tccccggcga
     1321 agaccactac cttggtgtcg ggcttgtgga gcttgatccc cgtcgcccat gcgagggtcc
     1381 tgccgtggac gccgtggagg cagtccatgt tgatgtagac gggaatccgg gcagtgcagc
     1441 ccacgccgcc gatgcccacc atggagccga ggtcgaggcc gagggcttcc acggcccgga
     1501 gaaaggcgct cacaacctgg ccgcagccgc agccggggca gaagaaatg
//
LOCUS       DAOTHD010000594         1548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_96072_length_1548_cov_4.785666, whole genome shotgun sequence.
ACCESSION   DAOTHD010000594 DAOTHD010000000
VERSION     DAOTHD010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96072_length_1548_cov_4.785666"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="P5219_09540"
     CDS             complement(<1..327)
                     /locus_tag="P5219_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27045.1"
                     /translation="MVRDDKHFHLLQRMLSFNLRRSLSLETKKNRCVPGKKKTFNILL
                     REEVTVFAREDLPPAENVEIRHRTVGQHTGRVVFASEPKRVEGRAERDCLSVYGEFQA
                     VSGEEEE"
     gene            complement(504..1523)
                     /locus_tag="P5219_09545"
     CDS             complement(504..1523)
                     /locus_tag="P5219_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRX27046.1"
                     /translation="MKQVRVFSLLLGIAFVVSGMFVCAGPLAAQEYTLQLAGAYPSTG
                     ETPRALATEKIKELIEKKSNGKIKVQIYLDNQLGGDREILEGCQLGDIAMVSQTTAPQ
                     VGFIPELAIFDIPMLYENLDVARKALSGTFRAKLEEWYDRAGLKLLLFEPIYYRETTT
                     SKPINTVADFGGVKIRTMENEYHMAFWKALGANPTPLSFAELYVALQQGTVDAQENPY
                     EIIWSSKFYEVQKYLVNTHHIAFILSININKGIYDGMSDEYKKIIDESMKEASEYLFD
                     LAKAKNDAMLASLKQSGMTIIDPDPALQAELKKAAAETEKLIRKNVGDAVVDEVLKSA
                     EEAKK"
BASE COUNT          354 a          414 c          377 g          403 t
ORIGIN      
        1 ctcttcctcc tctccggaaa cggcctggaa ctcaccgtaa acggagagac agtcccgctc
       61 cgctctccct tcgactcgct tcggttcaga ggcgaacacg acgcgtcctg tatgctgacc
      121 gacggtccgg tgacggattt caacattttc tgcaggaggg agatcctcac gtgcgaatac
      181 cgtcacctct tctcgaagaa ggatattgaa cgtctttttc ttcccgggga cacaacgatt
      241 cttctttgtc tccaaggaga ggctacggcg gagattgaag gaaagcatcc tctgaaggag
      301 atggaaatgc ttgtcgtcac ggaccactct tggggacagc cgttgaccct tcaatacggg
      361 gagaaggcac gccttctttt cattacttta acaaaacgct aaaaacgtgt aaggaaaaac
      421 tccatgcgct gaaatagctg aaagaagaag cccggatccc aacgacccgg gcttcttctt
      481 tcagaaaaac aaggagcgaa tcgctatttt ttcgcttctt cggctgactt caacacttca
      541 tcgacgacag cgtcccctac atttttacgg atcaactttt cggtttccgc agcagccttt
      601 ttcaattccg cctgaagggc gggatcagga tcgatgatgg tcatcccgct ctgtttcagg
      661 gaagccagca tcgcgtcatt tttcgccttg gcaaggtcaa aaagatactc cgacgcttcc
      721 ttcatacttt cgtcgatgat ctttttgtat tcatcggaca tgccatcata gatacccttg
      781 ttgatgttga tgctcagaat aaacgctatg tgatgggtat tgacgaggta tttttgaact
      841 tcgtagaatt tgctggacca tatgatctcg tagggatttt cctgagcatc gacggttccc
      901 tgctgaagag cgacgtatag ctccgcgaag ctcagagggg taggattggc ccccaaagct
      961 ttccagaaag ccatgtggta ttcgttctcc atggtgcgga tcttgactcc gccaaagtcg
     1021 gcaacggtat tgatgggttt gctggtggtg gtttcccggt aatagatggg ttcgaaaagg
     1081 aggagcttga gtcccgccct gtcgtaccat tcctcgagct ttgcccggaa cgtcccggaa
     1141 agcgctttcc tcgctacatc caggttttca tacagcatgg ggatatcgaa aatggcgagc
     1201 tcaggtatga atcctacctg gggagctgtc gtctgagaga ccatggcaat gtcgcccagc
     1261 tggcatcctt cgagtatctc ccggtcgccg ccaagctgat tatcgaggta aatctgcacc
     1321 ttgatcttgc cgttcgattt cttctcgata agctccttga tcttttccgt agccagcgct
     1381 ctgggagttt ctccggtgga tggataggcc ccggccaact gcagcgtata ttcctgggcc
     1441 gccagcggac ctgcacacac gaacattccc gacacaacaa aagcaattcc caatagaagc
     1501 gaaaacaccc tgacctgctt catttcatac ccctcctttt tcaagttg
//
LOCUS       DAOTHD010000595         1544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_96454_length_1544_cov_2.647414, whole genome shotgun sequence.
ACCESSION   DAOTHD010000595 DAOTHD010000000
VERSION     DAOTHD010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96454_length_1544_cov_2.647414"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="P5219_09550"
     CDS             <1..284
                     /locus_tag="P5219_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide transporter"
                     /protein_id="HRX27047.1"
                     /translation="AGLNPEILRQLVIASVFGAALQFAGGAKKALGILFATGLLIRNP
                     IYGAGLLVALLIRLPLEKNFKPELEIYGGGLVAGDGIYGFVNALIRSFM"
     gene            284..967
                     /locus_tag="P5219_09555"
     CDS             284..967
                     /locus_tag="P5219_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HRX27048.1"
                     /translation="MTKQRIGLIRVLTTADEKLLNLHGELVMQYFPCFDVVSRCIPDQ
                     PEGIHDAETERIAVPKVLALAQEMEREGMDAVIVSCAGDPAVEEASRSLRIPVIGAGR
                     AAASAALVTGLPVGVLGITKVVPEAMSRILGKNLAADAVPEGVESTLDLMKPEGMQAL
                     VVAGRFLAGRGARMLVLACTGMSTIGAAGILRKKTGIPVIDPVRAQAAAAWTILGMRG
                     GDSRWQKEP"
     gene            949..>1544
                     /locus_tag="P5219_09560"
     CDS             949..>1544
                     /locus_tag="P5219_09560"
                     /inference="COORDINATES: protein motif:HMM:NF017812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 family protein"
                     /protein_id="HRX27049.1"
                     /translation="MAKRTITKETGEAAILGGAVLGGGGGGSMEKGRLNLRGALEAGE
                     VVLADIDDISPDTVLITGSAVGAPAAAEARVEPKDYLRVVEILKENGCPAPGGFIPNE
                     CGGSSITNGWVPAALMGLPVVDALCNGRAHPTGVMGSMGLHRDPGYVSRQAAAGGDRE
                     KGLYLELHVAGKLGSASSMIRAAADKSGGLVAVARNPVA"
BASE COUNT          286 a          457 c          548 g          253 t
ORIGIN      
        1 aggcggggct gaacccggaa attctcaggc agctcgtcat cgccagtgtc ttcggtgcgg
       61 cgctgcagtt tgcgggaggg gcgaaaaagg ccctggggat cctcttcgcc acgggactgc
      121 tcatccgcaa tcccatttac ggcgccggtc tcctggtggc ccttctcatc aggcttcccc
      181 ttgaaaagaa cttcaagccg gaacttgaaa tctacggcgg cggcctcgtg gcgggcgacg
      241 gcatttacgg gttcgtgaac gccctcataa ggagcttcat gtaatgacaa aacagcggat
      301 cggcctcatc cgcgtgctga ccacggcgga tgaaaagctc ctgaaccttc acggcgagct
      361 cgtgatgcag tatttcccct gcttcgacgt ggtctcccgg tgcatcccgg accagcctga
      421 gggaattcat gacgccgaga cggagcggat cgccgttccc aaggtgctcg ccctcgcgca
      481 ggaaatggag cgggaaggga tggatgctgt tattgtgagc tgtgcaggcg acccggcggt
      541 ggaggaggcc tcccggtccc tccggattcc cgtcatcggc gcaggaaggg cggccgcctc
      601 ggccgccctt gtcacaggac tgccggtggg cgtcctggga atcacgaagg ttgtgccgga
      661 ggccatgagc cgcattctgg ggaaaaacct cgcggcggac gcggtgcccg aaggggtgga
      721 gtccacgctg gaccttatga agcccgaagg gatgcaggct ttggtcgtcg caggacggtt
      781 tcttgccgga aggggagcga ggatgctcgt tcttgcctgc accggcatgt ccaccatcgg
      841 ggcggccggg atcctcagga aaaaaaccgg cattcccgtc atcgatcccg tgagggccca
      901 ggccgctgcg gcgtggacca tcctgggcat gagaggagga gacagccggt ggcaaaaaga
      961 accataacga aagaaacggg agaagccgcc atactgggcg gagcagtcct gggaggcggc
     1021 ggagggggca gcatggagaa ggggcgcctc aacctcaggg gcgccctgga ggccggagag
     1081 gtggtcctgg ccgatatcga cgacatttct ccggacacgg tgcttatcac cgggtcggcc
     1141 gtcggggccc ccgcggcggc ggaagcgcgg gtggagccga aggactatct ccgggtcgtg
     1201 gaaatactga aagaaaacgg atgtccggct ccgggagggt ttatccccaa cgagtgcggc
     1261 ggaagctcca tcaccaacgg atgggttccc gcggccctca tgggcctgcc ggtggtggat
     1321 gccctctgca acggccgggc ccaccccacg ggagtcatgg gctccatggg gctccacagg
     1381 gaccccgggt acgtctcccg gcaggcggct gccggaggag accgggagaa ggggctctac
     1441 ctggaactgc acgtggcggg gaagctcggc agcgcgtcct ccatgatccg ggcggcggcg
     1501 gataaatccg gaggcctcgt tgccgtggcc cggaacccgg tggc
//
LOCUS       DAOTHD010000596         1543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_96499_length_1543_cov_4.367608, whole genome shotgun sequence.
ACCESSION   DAOTHD010000596 DAOTHD010000000
VERSION     DAOTHD010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96499_length_1543_cov_4.367608"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="P5219_09565"
     CDS             complement(<1..659)
                     /locus_tag="P5219_09565"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRX27050.1"
                     /translation="MRQRCAVLAVFLLLGLLFLPAAWGAVELKLGHFGPENHPATVAA
                     ARFAENVEKRTNGEVRIVVYPNNALGSPPEVLEQVIMGVIDMSLSGQDQIAKHVKLFD
                     SVAIPFSMQGYDHADRILDGPFKEWAAPELEKIGLVYINSWEWGFRQITNSKRPILSP
                     DDVKGLKIRTPPAMAYQAAIEALGGNVQTIAFGELVMAMRQGVVDGQENPIGVIYDLK
                     L"
     gene            1024..>1543
                     /locus_tag="P5219_09570"
     CDS             1024..>1543
                     /locus_tag="P5219_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRX27051.1"
                     /translation="MLEKIKELAASSKSDTAAFRHHLHTHPEIAWQEVETTKAVVEEL
                     KKIGCDIVKIGFGGTECGVVADLRGGKPGKRVALRGDMDALPMNEDNDLPYRSQKDGV
                     MHACGHDAHTAMLLGAAKVLSEIRDDIPGTVRFLFQPAEESGLKSGAKAMVEEGALDG
                     VDAVAGIHCWSSA"
BASE COUNT          343 a          457 c          445 g          298 t
ORIGIN      
        1 tagagcttca ggtcgtagat aacgccgatg gggttctcct gcccgtccac cacgccctgg
       61 cgcatggcca tgacaagctc gccgaaggcg atggtctgca cgttgccgcc aagggcctcg
      121 atggccgcct ggtaggccat ggccggcgga gtgcggatct tcagcccctt cacgtcgtca
      181 ggactcagga tgggacgctt ggagttggtg atctgccgga acccccattc ccagctgttg
      241 atgtacacaa ggccgatctt ttcgagctcc ggggcggccc attccttgaa gggaccgtcg
      301 agaatcctgt cggcgtggtc gtacccctgc atggaaaaag gtatggccac cgagtcgaag
      361 agcttcacgt gcttggcgat ctggtcctgg cccgaaaggc tcatgtcgat gacgcccatg
      421 atgacctgct ccagcacttc gggggggctt cccagggcgt tgttcgggta gacaacgatc
      481 ctgacctcgc cgttggtcct cttttccacg ttctcggcaa aacgggcggc cgcgacggtg
      541 gcgggatgat tctccggacc gaaatggccg agcttcagct ccaccgctcc ccacgccgcc
      601 ggaagaaaca ggagccccag cagaagaaac accgcaagaa ctgcacatct ttgcctcaac
      661 acaaacccct ccttcgggtt acatatcgcg gccctcggcc gcggaaaagc ccttttctga
      721 caaaacggcg gaaaatcccg ccccggagct gatgtttcat ccaataaaac attatttcaa
      781 atatagccgc ttaattttcg taaaagtcga aagctttgtc aataccccgg gggaatactt
      841 acttgactta acaggctttt ccgtcattgc acaagcctgc ggcggctttc cagcacaggc
      901 tttccatgaa aaaaacattc acggcggcac gtcagaattc tcttgatttc ccccaaaaag
      961 ggtgatagtg tgtattttgc attttcgtca gaccaacttt gagtgaacga ggtgaccttt
     1021 tcaatgctcg aaaagatcaa ggagctagcg gcatcatcca agagcgacac tgcggcgttc
     1081 aggcatcatc ttcatacaca ccccgaaatc gcctggcagg aagtggaaac aacgaaggcg
     1141 gtagtcgaag aattgaaaaa aatcggatgc gacatcgtga agatcggctt cggcggcacc
     1201 gaatgcggcg tggtggccga cctcaggggc ggaaagccgg gaaagcgggt ggccctccgg
     1261 ggcgacatgg acgcccttcc catgaacgag gacaacgacc ttccctacag gtcgcagaag
     1321 gacggcgtca tgcacgcctg cggccacgat gcccacacgg ccatgctcct cggggcggcg
     1381 aaagtgctgt cggaaatcag ggatgacata ccgggcacgg tgcgcttcct gttccagccc
     1441 gccgaggaga gcggcctgaa atcaggcgcc aaagccatgg tggaggaagg ggccctcgac
     1501 ggcgtggacg ccgtggcggg catccactgc tggtcctccg ccc
//
LOCUS       DAOTHD010000597         1542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_96623_length_1542_cov_2.901816, whole genome shotgun sequence.
ACCESSION   DAOTHD010000597 DAOTHD010000000
VERSION     DAOTHD010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96623_length_1542_cov_2.901816"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..769)
                     /locus_tag="P5219_09575"
     CDS             complement(80..769)
                     /locus_tag="P5219_09575"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRX27052.1"
                     /translation="MKCSQCGADLPDGANFCHACGAEQETPAGSGKGKGSRVGYSEKI
                     HDPAFQKYLKNTNRWSSIFSAGLAVIAVAGFFIAGETGAGGMENPESLYIGFGIGGMF
                     LAIAFFQILGRKRSTTWDGVVTDRTVKQKRRKQSTGGDKNDYYWVNYTEYTVEISSDG
                     GKKHFIVVDDDDTLYNYYRVGDRVRHHGGLKTYEKYDKSGDSIIFCNACATKCDIGDD
                     VCFRCGCPLLK"
     gene            1121..>1542
                     /locus_tag="P5219_09580"
     CDS             1121..>1542
                     /locus_tag="P5219_09580"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRX27053.1"
                     /translation="MKLHYTRFIAACLLFGSNGIVASHIALGSHEIVLWRTLIGSLFL
                     LAVFVLTGGRFGGLRSRKHLTFLLFSGVAMGASWMFLYEAYRRIGVGPATLAYYCGPV
                     IVMVLAPVIFREKLAFSRILGFGAVLLGMVFVNGDEVIR"
BASE COUNT          284 a          472 c          446 g          340 t
ORIGIN      
        1 aaaaatccag gattcaggaa cgttccggta gaagaagtga gcccgccgga cctccgacgg
       61 gctcgttgcc gttctctttc tatttcagca gcgggcagcc gcaccggaag cagacatcat
      121 cgccgatatc gcatttcgtg gcgcaggcgt tgcagaagat gatactgtcc ccggacttgt
      181 cgtatttctc gtacgttttc agcccgccgt ggtgccgcac cctgtccccc acccggtagt
      241 agttgtagag ggtgtcgtcg tcgtcgacga caatgaaatg cttctttccg ccgtcggagc
      301 tgatctccac cgtgtattcc gtgtagttca cccagtaata atcgttcttg tcgccgccgg
      361 tgctctgctt gcggcgcttc tgcttcaccg tcctgtctgt gaccacgccg tcccaggtgg
      421 tgctcctctt tctgcccaaa atctggaaga aggcgatggc gagaaacatg cccccgatcc
      481 cgaagccgat gtagagggac tcggggtttt ccatgccccc ggcgccggtc tccccggcga
      541 tgaaaaagcc ggccacggcg atgacggcaa gacctgccga aaagatggag gaccaccggt
      601 tggtgttctt caggtatttc tgaaacgcag ggtcgtggat cttttccgag taccccaccc
      661 ggctgccttt tcccttcccg gaacccgcag gagtttcctg ttccgccccg caggcatggc
      721 agaaattcgc cccgtccggc agatccgctc cgcattgact gcacttcatg gacatccctc
      781 ctccgttttc ctctgtctga ccggttgccc agagacgacg attcccttac gatgcgcgga
      841 tttcattgta ccgtaaaccg aacggaggcg gaatcgtccc ttcgaagtaa agccagccgg
      901 atcggactct cccgcccgca gtttctgccg cggagctttc ttctttcagt caggccacgg
      961 ctggtataaa attacattct ggttccggag aaagagaacg tcccggaaag agccgaacag
     1021 accggcaggg attcctgtac ccccacacgg ccgacacctt accggatgga ggcggggacg
     1081 aggagccgcc ggactgccgc aaggagagag aacagaaaaa atgaagctgc attacaccag
     1141 gtttatagcc gcgtgcctgc tgttcggctc caacggcatc gtggcaagcc atatagcact
     1201 gggcagccac gagatcgtcc tgtggaggac cctcatcggc agtctctttc ttctggcggt
     1261 gttcgtgctg accggggggc ggttcggagg gctgcgcagc aggaagcatc ttacattcct
     1321 gcttttttcc ggcgtggcca tgggcgcgag ctggatgttc ctgtacgagg cctaccggcg
     1381 gatcggcgta gggcccgcca ccctcgcgta ctactgcggc ccggtcatcg tgatggtcct
     1441 cgcccccgtg atattcaggg agaagctggc cttttcgagg atcctcggct tcggggccgt
     1501 ccttctcggg atggtcttcg tcaacgggga cgaggtgatc cg
//
LOCUS       DAOTHD010000598         1540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_96786_length_1540_cov_3.060606, whole genome shotgun sequence.
ACCESSION   DAOTHD010000598 DAOTHD010000000
VERSION     DAOTHD010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96786_length_1540_cov_3.060606"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="P5219_09585"
     CDS             <1..345
                     /locus_tag="P5219_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="HRX27054.1"
                     /translation="SFIAKGTYMDMMYMPDALAAVVKLMEADPSKLVHRNAFNISAMS
                     FEPEQIAASIRKIMPGFTMEYDVDPLRQGIAESWPDSLDCTAAREEWGFAPEYDLDRM
                     TEDMLRRLKEKN"
     gene            complement(718..1356)
                     /locus_tag="P5219_09590"
     CDS             complement(718..1356)
                     /locus_tag="P5219_09590"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRX27055.1"
                     /translation="MKRIFLVRHGETDWNREGRFQGQMDIPLNGTGLVQARSVAEALK
                     DVSLDRIVASPLSRARETARPLAELKGLRAELSEGLIEIGHGLWEGRTSGEVEAEWPG
                     RLEAWHTRPETVVMPGGESLEDVQNRAWPALLRIAEGKGENIAVFTHDAVLKVLLMNV
                     LDCPLSSFWKFQLANGSITLLEQSEKGWRIPLMGEAGHLGSTFGIRAEQKGL"
     gene            complement(1356..>1540)
                     /locus_tag="P5219_09595"
     CDS             complement(1356..>1540)
                     /locus_tag="P5219_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRX27056.1"
                     /translation="TILGEAGSNIANFALGRKNGSGLAVGALQIDNDIPLKVIDTFKA
                     DADLLWAVKVNFSEAL"
BASE COUNT          308 a          485 c          452 g          295 t
ORIGIN      
        1 agcttcatcg cgaaggggac ctacatggac atgatgtaca tgcccgacgc cctcgcggcc
       61 gtggtgaagc tcatggaggc cgacccgtcg aagctggttc accggaacgc cttcaacata
      121 tccgccatga gcttcgagcc cgagcagatc gccgcgtcca tccgcaagat catgcccgga
      181 ttcaccatgg agtatgacgt ggatccactc cggcagggca tcgccgaatc ctggcccgac
      241 tcgctggact gcaccgcggc ccgggaggag tgggggttcg ccccggagta cgacctggac
      301 cggatgacag aggacatgct ccgccgactg aaggaaaaga actaagccaa aagaagaagg
      361 gcagcccccg gaaatccggg ggctgccctt cttcttttcc ctaaaagccc cgcccggacg
      421 acacaatgca tgaatgatat gcttttaccg tcagtttata ctgaaaacag agcaggagag
      481 cctcttcatc gtgcaggcca gacgcaacga tgcagtacgc acaagttttt gcatagtaaa
      541 aactatgaga gcaacgggta ttgggatttt tggcaaaaac aaagcagtat tccgggacag
      601 gactgcgaaa ccatctgcgg cctgctcgac agtatcaggg gcaaactgga cgacgacggc
      661 ggaagcgaat ccgacagcgg ctcgggacag gtggattgcc ccgtcctttt cctcacctca
      721 cagccccttc tgttccgccc gaattccgaa agtgctgcca aggtgtcccg cctcgcccat
      781 cagggggatg cgccacccct tttcgctctg ctccagcagg gtgatgctgc cgttggcgag
      841 ctggaacttc cagaagcttg agagaggaca gtcaagcacg ttcatcagca ggactttcag
      901 cacggcgtcg tgggtgaaaa cagcgatgtt ttcacccttt ccttccgcaa tccgaagcag
      961 ggcgggccag gcgcggttct gcacgtcctc gaggctctcc cctccgggca tgaccaccgt
     1021 ttcgggccgg gtgtgccagg cttcgagcct tcccggccac tccgcctcca cttcgccgga
     1081 ggtgcggcct tcccagaggc cgtgccctat ttctatgagc ccttcggaca gttccgcccg
     1141 cagtcccttc agttcagcaa gggggcgggc ggtctcccgg gccctggaga gggggctggc
     1201 cacgatcctg tccagggaaa cgtctttcag cgcctccgcg acggaacggg cctggaccag
     1261 ccccgttccg ttcaggggga tgtccatctg gccctggaac cgtccttccc tgttccagtc
     1321 cgtttccccg tgccgcacga gaaagatccg cttcatcaca gggcctcgga gaagttcacc
     1381 ttcacggccc agagaaggtc cgcgtctgcc ttgaaggtat cgatgacctt caggggaatg
     1441 tcgttgtcga tctgcagggc tccaacggcg agaccgcttc cgttcttcct gcccagggcg
     1501 aagttggcga tgttggaccc ggcctcgccg aggatggtgc
//
LOCUS       DAOTHD010000599         1537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_97056_length_1537_cov_3.413630, whole genome shotgun sequence.
ACCESSION   DAOTHD010000599 DAOTHD010000000
VERSION     DAOTHD010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97056_length_1537_cov_3.413630"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..1214
                     /locus_tag="P5219_09600"
     CDS             276..1214
                     /locus_tag="P5219_09600"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRX27057.1"
                     /translation="MKAFRTLSSALLVLLLLAFAAGAETAKTDDPAPEVKAPAAEAKA
                     PVDPETVLARVGETEIRMKDVREILGKLDPQRASMYDNEMGHRAILEEMVNMELFLLL
                     GKELEVEKDPGFAEMLESVKEDIIRRFAVDRVMKDVKVTPEDIAGYYEKNREHFAVPE
                     SVRASHILVSGDQEMQKVMEDLKAGMSFEDAAKKHSTCASKEQGGDLGYFSKGDMVKE
                     FNDAVFAMKVGEVTAEPVKTKFGLHLIKLVEKKEASVRPLDEVKEELREALENDKKAK
                     VYQDELSRLREKYKVEIVGEKKEEKKEEEKAGEAGK"
     gene            complement(1287..>1537)
                     /locus_tag="P5219_09605"
     CDS             complement(1287..>1537)
                     /locus_tag="P5219_09605"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRX27058.1"
                     /translation="TIVVPSPPSRESVAGLAERILRRVAKKALLQGKEISVTASIGIS
                     LFPDDGTTVQDLMKHADVAMYTAKDQGKNRFAFRDRLP"
BASE COUNT          351 a          386 c          484 g          316 t
ORIGIN      
        1 gatgctccgc cgtcctggtt ctgctggggt tcagaagaat ccgcctggga agtactctca
       61 agggtgttat tgatatcgtg ggagcagggg ccattgccat tatgggatgg tcactgggca
      121 acccctgacc ccgctttttc ccttttcccc cgcgtgcgga ttgcccccga gggggggcgg
      181 ttcagcattt ctgccgtttt tttcccctcc gtttgtggta cacttttccg gatttgatgc
      241 tgtttactat gttcaccatc cagggaggaa tttctatgaa agcgtttcgt actctgagct
      301 ccgcactgct cgttctgctt cttctggcct ttgccgccgg cgccgaaacg gcaaagaccg
      361 acgacccggc ccccgaggtc aaggcccccg ctgccgaagc aaaggctccc gtcgatccgg
      421 agaccgttct ggcccgggtg ggggagaccg aaatccggat gaaggatgtg agggaaatcc
      481 tgggcaagct cgatccccag agagcctcca tgtacgacaa tgaaatggga cacagggcta
      541 tccttgagga aatggtcaac atggagcttt tccttctcct cggaaaagaa cttgaggtgg
      601 agaaagaccc cggtttcgcc gagatgctgg aaagcgtgaa agaggatatt atccgcaggt
      661 ttgcggtcga cagggtgatg aaggacgtga aggtcactcc tgaagatatc gccgggtact
      721 atgaaaagaa cagggagcat tttgccgttc ccgagagcgt gagagcctcc cacattctgg
      781 tgagcggcga ccaggagatg cagaaggtca tggaagacct gaaagcggga atgtccttcg
      841 aggatgcggc gaagaagcat tccacctgcg cgtcgaagga gcagggaggc gacctcggat
      901 atttctccaa gggggatatg gtgaaggaat tcaatgatgc cgtattcgcc atgaaggtcg
      961 gagaggtcac ggcagaaccg gtgaaaacca agttcggcct tcacctgatc aagcttgtcg
     1021 agaagaagga agcctcggtc cggcctctgg acgaggtgaa ggaagagctc agggaagccc
     1081 ttgaaaacga caaaaaggca aaggtctacc aggacgagct ttccaggctg agagagaagt
     1141 acaaggttga gatcgtcgga gagaaaaagg aagaaaagaa agaggaagaa aaagcgggag
     1201 aagccggaaa gtaggtattc ctccggctga ggtaaagaga aagggggagg gcccggtccc
     1261 gggccttccc cctttctctt tcggtctcag ggcagcctgt cccggaaagc gaacctgttt
     1321 ttgccctggt ccttggcggt gtacattgcg acatcggcgt gtttcataag gtcctggacc
     1381 gtggttccgt catcggggaa caggctgatt ccgatgctgg cggtcacgga aatctccttc
     1441 ccctggagga gcgctttttt cgccaccctc cggaggatcc gttccgcgag gccggccacg
     1501 gactcccggg aaggcgggga cggtacgacg atggtga
//
LOCUS       DAOTHD010000600         1535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_97219_length_1535_cov_4.020270, whole genome shotgun sequence.
ACCESSION   DAOTHD010000600 DAOTHD010000000
VERSION     DAOTHD010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97219_length_1535_cov_4.020270"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="P5219_09610"
     CDS             <1..322
                     /locus_tag="P5219_09610"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRX27059.1"
                     /translation="PGRGWYVAGGLFYGIVMGMSFPIVLALMPDIFPDHLRPKGISLC
                     LFAIDLGFILSPFFLGYGGQLFGLGNTLSVTGVLGLLGGSLLHLAWKAKKKEGTPKGG
                     CSVI"
     gene            466..1188
                     /locus_tag="P5219_09615"
     CDS             466..1188
                     /locus_tag="P5219_09615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRX27060.1"
                     /translation="MNDRTISWYDEHAEGLASRYDQADAESLHSLLRKWIPSGSSVLE
                     IGCGSGRDALFLASMECSVTATDGSDAMLQAARQNLRKKAERNVSFEPALFPLPAGHP
                     LLRRTFDAITAIAVLMHISDSDLFTFASQVKILLKDQGIFFCSFSFGERKEKDDRFFE
                     NRTPEEVQHLFEQSGFRLLARVETKDGLGRDILWMTMVFSLRENELSDPYGEECHGLL
                     EKTSPETGRPNSENKDEFTGTG"
     gene            complement(1172..>1535)
                     /locus_tag="P5219_09620"
     CDS             complement(1172..>1535)
                     /locus_tag="P5219_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27061.1"
                     /translation="ATVGFLLGGVFLRVMDRVPEEAKIAAVLHDVVEDSTVSIEDLRA
                     EGFPERVVEAVEALTKRPGETYALFILRAASNDIARTVKLADLFDNCDLSRIPDPGPE
                     DYERLKKYREAMDLIRSL"
BASE COUNT          329 a          445 c          426 g          335 t
ORIGIN      
        1 tcccgggcgg ggctggtatg tagctggagg gctgttctac ggcatcgtca tggggatgtc
       61 ctttcccata gtgctcgccc tcatgccgga catctttcca gatcaccttc gccccaaggg
      121 gatctccctc tgtctcttcg ccatcgacct gggatttatt ctctccccgt tcttcctggg
      181 atacggcggg cagcttttcg gcctcggaaa caccctctca gtcaccggcg tgcttggcct
      241 tctgggcggt tccctgctgc acctcgcatg gaaagcaaaa aagaaagaag gcacccccaa
      301 gggaggatgc tccgtgatct gagagcggag aacacgtaag ttgagaagaa ttccggcagc
      361 attggagggt tctttttcaa tcatccccga atcttttcag gccgcagaaa ccgggcgttt
      421 caatgacctg tacgactggt ttcaggcacg gcaggaggcc cgattatgaa cgaccggacc
      481 atctcatggt acgacgaaca tgctgagggg ctcgcttccc ggtacgacca ggcggatgct
      541 gaatcccttc attcccttct ccgaaaatgg atcccatccg gatcatcggt tctggaaata
      601 ggctgcggct ccggcaggga tgccctcttc ctcgcatcca tggaatgttc ggttactgcc
      661 acagacggca gcgatgccat gctgcaagcc gcccggcaga accttcggaa gaaagcggaa
      721 agaaacgtct ccttcgagcc ggccctcttc ccgctgccgg cgggtcaccc cctgctgcgc
      781 cgcaccttcg acgcaataac ggctatagcg gtgctgatgc atataagcga ttccgatcta
      841 ttcaccttcg ccagccaggt gaaaatcctc ctgaaggatc agggaatctt tttctgctcg
      901 ttctctttcg gggaacggaa agaaaaagat gatcgttttt ttgaaaaccg gacgccggaa
      961 gaagtgcagc atctcttcga acagtctggt ttccggctgc tggccagggt ggaaacaaaa
     1021 gacggtcttg gaagggatat tctctggatg accatggtgt tttcgcttcg ggaaaatgag
     1081 ctgtcagacc catacgggga ggaatgccac ggcctccttg aaaaaacttc cccggagacc
     1141 ggcaggccaa acagtgaaaa caaggatgaa ttcacaggga ccggatgaga tccatcgctt
     1201 cccggtattt tttcagccgt tcataatcct ccggtccggg gtcggggatg cgggagaggt
     1261 cgcagttgtc gaaaaggtcg gcaagcttga cggtccgggc aatgtcgttg gatgccgccc
     1321 ggaggatgaa cagcgcatag gtttcgcccg ggcgtttcgt aagggcctcc acagcctcga
     1381 ccaccctttc gggaaagcct tcggccctca gatcctcaat cgaaactgtt gagtcttcta
     1441 ccacgtcatg aagcacggcg gcaatttttg cctcttcggg gacccggtcc atgacccgca
     1501 ggaacacgcc ccccagcagg aaaccgaccg tcgcc
//
LOCUS       DAOTHD010000601         1535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_97253_length_1535_cov_2.761486, whole genome shotgun sequence.
ACCESSION   DAOTHD010000601 DAOTHD010000000
VERSION     DAOTHD010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97253_length_1535_cov_2.761486"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="P5219_09625"
     CDS             <1..688
                     /locus_tag="P5219_09625"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRX27062.1"
                     /translation="SGMEALLRWHAPDRVIPPWEFIPLAEETGLILPLGEWVFREVCR
                     QVSQWASEGHPPPEVAVNLSARQLHQEGLPAFLKGVMDEYAVDPSRIGVEITESGIME
                     DLVDSVSVLSGIKDLGMTVYVDDFGTGYSSLNYLKRLPIDVLKIDKSFVDGVLTDRND
                     AAITRAVIVLGRSLGMSVLAEGVETRDQLEFLRQNGCDGIQGYLLSPPLPPEDTLRFR
                     KTGHTPLFPA"
     gene            795..>1535
                     /locus_tag="P5219_09630"
     CDS             795..>1535
                     /locus_tag="P5219_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRX27063.1"
                     /translation="MQHIRSLSLFDQGDHRFIMLGWEEQEEEVAVQTNQYVISSGGEV
                     VLLDPGGAHVFPRVLANVAELFELKNIRHIFYSHQDPDVSSGITLWLSMAEKASVHIS
                     GLWVRFLPHFGIYDVKRISPIPDKGETLSLANGKKLVILPSHFLHSTGCFSLYDPASK
                     ILFSGDIGAAIFPRGGRYPVAEDLDSHLKYMEGFHRRYMASNAACRRWVKMVSALDVE
                     HIAPQHGAFIKGRQNVKKFLSWFENLACG"
BASE COUNT          282 a          458 c          471 g          324 t
ORIGIN      
        1 gtccggcatg gaggccctgc tccggtggca tgctcctgac cgggtgattc cgccctggga
       61 attcatcccc ctggcggagg agacggggct tatccttccc ctgggcgaat gggtcttcag
      121 ggaggtttgc cgccaggttt cccagtgggc gtcggagggg catcctcctc ccgaggtggc
      181 ggtgaatctg tctgcccggc agctccacca ggaggggctg cccgccttcc tgaagggggt
      241 catggatgaa tatgccgtgg atccctcacg gatcggcgtc gagatcaccg agtcggggat
      301 catggaggac ctggttgatt cggtgtctgt cctctccggg atcaaggacc tggggatgac
      361 ggtctacgtg gacgacttcg ggaccgggta ttcctccctg aactatctca agcgccttcc
      421 catcgacgtg ctgaagatcg ataagtcctt cgtcgacggc gtgctcaccg acaggaatga
      481 cgcggccatc accagggccg tcatcgtttt gggtcgttcc ctgggcatgt cggtgctggc
      541 ggagggggtg gaaacgcggg atcagctcga attcctgagg cagaacgggt gcgacggaat
      601 ccaggggtat ctgctgagcc ctcccctgcc tccggaggat accctgcggt tcagaaaaac
      661 ggggcatacc cctttgtttc cggcataggt caggatataa tcacagcata acgttccggg
      721 cgacaccttc tccccggggc tatgacagcc gacaatgaaa tccaattgtt tatttcggga
      781 aggagttggg ttatatgcag catatacgtt ccctttccct gttcgaccag ggcgatcacc
      841 ggttcatcat gctcggctgg gaggagcagg aagaagaggt ggccgtccag acgaaccagt
      901 acgtcatttc ttccggaggc gaggtggtgc ttctcgatcc gggcggagcc cacgtcttcc
      961 cccgtgtcct ggccaacgtc gcggaactgt tcgaactgaa aaacatcagg cacatcttct
     1021 attcccacca ggatcccgac gtgtcctcgg gaatcaccct gtggctctcc atggcggaaa
     1081 aggcctcggt ccacatctcc gggctgtggg tgcgcttcct gccccatttc ggcatctatg
     1141 acgtgaagcg catctcgccg atccccgata agggggagac cctttctctc gccaacggga
     1201 aaaaactggt tatcctgccg tcccactttc ttcactccac agggtgtttc tccctctacg
     1261 atccggcgtc gaaaatcctc ttcagcggcg acatcggcgc ggccatcttt cccagggggg
     1321 gccggtaccc cgtggcggag gacctggact cccacctgaa gtacatggag ggattccaca
     1381 ggaggtacat ggcgtccaac gccgcgtgcc gccgatgggt gaaaatggtg tccgcactgg
     1441 acgtggagca catcgccccg cagcacggcg ccttcatcaa ggggcggcag aacgtgaaga
     1501 agttcctttc ctggttcgag aacctcgcct gcggg
//
LOCUS       DAOTHD010000602         1532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_97486_length_1532_cov_4.008802, whole genome shotgun sequence.
ACCESSION   DAOTHD010000602 DAOTHD010000000
VERSION     DAOTHD010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97486_length_1532_cov_4.008802"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="P5219_09635"
     CDS             complement(<1..239)
                     /locus_tag="P5219_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRX27064.1"
                     /translation="MAERKLHQLTVDELTFRISSLLLQDPVLQSVVVKGELAELKKHT
                     SGHVYFTLLGGESRISCALFRNYIPYVPQWPRNGD"
     gene            complement(205..705)
                     /gene="nusB"
                     /locus_tag="P5219_09640"
     CDS             complement(205..705)
                     /gene="nusB"
                     /locus_tag="P5219_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRX27065.1"
                     /translation="MIRTFLPQKRHRSREMALQLLYSLDLRRGQTPEQALELFPAEDD
                     PEIQEYARILTCGAWERRREIDDLIREHVTGWRPERMLAVDRAALRMAIFESVLSRMI
                     PVPVAISEAVELAKTFGAEESGKFVNGVLGRIVRALYPEGTDGGEQTKKQELPDGGTE
                     TPPADS"
     gene            complement(710..1192)
                     /locus_tag="P5219_09645"
     CDS             complement(710..1192)
                     /locus_tag="P5219_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27066.1"
                     /translation="MYLFWKRNTLGNVSVSAAGMRQFVRRYLTKPFSCDLVSLPAGED
                     CFYVMLSYPKGETPPGMETSERRIRGEMEKLGFSVRISWVEVEKTPFDPVGELIALAR
                     KPLAWALLGAAIAVLFMVGFKTFFWTLFWGAAFYFGTVFLFSEKGERILEKLKGIAGR
                     "
     gene            complement(1262..>1532)
                     /locus_tag="P5219_09650"
     CDS             complement(1262..>1532)
                     /locus_tag="P5219_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27067.1"
                     /translation="IKKALEQYTGYDVKAVNINVQGIYFQDKPIPAPAPEPAAEGEKP
                     AVSEIPVSVTEPFVDEEDDVEPEMTGKTGNEEKVEKPIPPEVEQL"
BASE COUNT          335 a          486 c          391 g          320 t
ORIGIN      
        1 tcgtcgccgt tccggggcca ttggggaaca tacggtatat aattccggaa gagagcgcag
       61 gaaatgcggc tctctccacc caggagcgta aagtagacgt ggccgctcgt atgtttcttc
      121 agttccgcaa gttcgccctt cacaaccacc gactgcaaca ccgggtcctg aagcaaaaga
      181 gacgaaatgc ggaacgtcag ttcgtcaact gtcagctggt ggagtttccg ttccgccatc
      241 gggcagctcc tgtttcttcg tttgctctcc tccgtcggtg ccttccgggt acagtgcccg
      301 gacaatccgt cccagcacgc cgttgacaaa tttcccggac tcctcggccc cgaaggtctt
      361 ggcaagttcc acggcctccg aaatggccac aggaacggga atcatccgtg agaggaccga
      421 ctcgaaaatg gccatcctca gggcggccct gtccacagcc agcatccgct cagggcgcca
      481 ccccgtgacg tgttcccgga taagatcgtc gatctccctt cgccgttccc acgcgccgca
      541 tgtcaaaata cgagcatatt cctgaatctc cgggtcatcc tccgcgggaa aaagctccag
      601 cgcctgttcc ggggtctgcc ccctgcgcag atccagcgaa tacaacagct gaagcgccat
      661 ctcccgggac cgatgacgct tctgcggcaa aaacgtgcga atcaatccat tacctcccag
      721 caatgccctt cagtttctcc agaatccgct cgcctttctc ggaaaaaaga aaaacggtac
      781 caaaataaaa agccgctccc cagaaaaggg tccagaaaaa ggttttgaat cccaccataa
      841 acaggacggc aatagccgcc ccgagcaaag cccatgccag gggcttcctg gcgagggcga
      901 tcagttcgcc cactggatcg aagggagtct tctccacctc gacccacgat attctcacgg
      961 agaagcccag cttctccatc tccccgcgta ttctcctctc ggacgtttcc atgccgggag
     1021 gggtctcccc cttggggtag gaaagcatga cataaaaaca atcctcgcct gcgggaagag
     1081 agaccaggtc acagctgaaa ggcttcgtca ggtaccgcct gacgaactgc cgcattcccg
     1141 ccgcagagac cgacacgttg ccgagggtat tccgcttcca gaatagatac atatccacca
     1201 cccctataag gagaagggcc ggtcacgtag gatggagggc cggccctctc aaggtccttc
     1261 gctaaagctg ttccacctcg ggcggaatcg gtttctcgac tttttcttca ttcccggttt
     1321 tcccggtcat ttccggttcc acgtcgtctt cttcatcgac gaagggttcc gtcacagaca
     1381 cgggaatctc ggacaccgca ggtttttccc cttcggccgc cggttccggt gcgggggccg
     1441 ggataggctt gtcctggaag taaataccct gaacgttgat gttcacggcc ttgacatcat
     1501 aacctgtata ctgctcgagg gccttcttga tg
//
LOCUS       DAOTHD010000603         1530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_97667_length_1530_cov_3.199322, whole genome shotgun sequence.
ACCESSION   DAOTHD010000603 DAOTHD010000000
VERSION     DAOTHD010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97667_length_1530_cov_3.199322"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1530
                     /locus_tag="P5219_09655"
     CDS             <1..>1530
                     /locus_tag="P5219_09655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRX27068.1"
                     /translation="LYGRIIGRIDSLPSVAAGDISLFASDLSEILKREMGIKSVAICL
                     NSGESRVPSPGEEKRSGGLSLSAFRCPIVFGGRNFGCFRLEYSDGDCSRRDEDALFLS
                     EVAGKAGMVLLNRERLDALEKLKESELFLKKAQEVARTGHWRIDIPGNRILWSEETYK
                     IFGVPSGSPVTLETFYSLVHPSDMTTVKEGWARTLRGEPYFLRHRILRGNESRWVEER
                     AEVEFDSGGVPVAGLGTVRDITLQVNSEQRLELYRQNLENLVAYRTADLENAMKRLDM
                     ERSFLTSILKSFSYPFCVIDASDYSLVIQNDAAAAFRPGARTCHDLFFGAEDPCSPSE
                     RVCPLREVVSTGLPFQEETSQGRNDAYYRRYCHPVFDRDGRVTHVIMYIIDISTDRML
                     REQLTHETARANELKERAEAANRAKSAFLSNMSHEIRTPLNAVIGFAHLLKNSPLDTV
                     QRDHADRLLDASRHLLRLVSDILDLSKIEANRMEPEIRDFEPSRHIDQVCALVAEDAA
                     G"
BASE COUNT          332 a          454 c          454 g          290 t
ORIGIN      
        1 cctctacggc aggatcatag ggcgcatcga ctcgctgccg tccgtggcgg cgggggatat
       61 ttcccttttt gcatcggatc tttcggaaat actgaaacgg gagatgggca taaaatcagt
      121 tgccatctgc ctgaacagcg gagaatcccg ggtgccttct ccgggagagg aaaaacgttc
      181 cggcgggctt tccctgtctg cctttcgatg ccccatcgtc ttcggcgggc ggaactttgg
      241 ctgtttccgg ctggagtaca gtgatggaga ctgttcccgg agagatgagg acgctctctt
      301 tctcagcgaa gtcgcaggaa aggcggggat ggtcctcctg aacagggaac ggctcgatgc
      361 cctcgagaag ctgaaagaga gcgaactgtt cctgaaaaag gcccaggaag tggcacggac
      421 aggccactgg cggattgaca ttcccggaaa caggattctg tggtccgagg aaacctacaa
      481 aatttttggc gtcccttccg gaagtcccgt cacgctcgag accttttatt ccctggtgca
      541 tccttcagac atgacaacag taaaggaagg atgggcaaga accctccggg gagagcccta
      601 ttttctccgc cacaggatcc tccgcggaaa cgagagccgg tgggtggagg agcgggcgga
      661 ggtggagttc gacagcggcg gggttcccgt cgccggtctg gggaccgtga gggacatcac
      721 cctccaggtg aattccgagc agcgcctcga actgtaccgc cagaacctgg agaacctcgt
      781 ggcctacaga acggcagacc tggaaaatgc catgaaaagg ctcgacatgg aacgaagctt
      841 cctcacttcc atactcaaat ccttttctta tccgttttgc gtcatcgacg catcggacta
      901 ttccctcgtg atacagaacg acgccgcggc ggccttccgc ccgggcgccc ggacctgcca
      961 cgacctgttc ttcggcgcgg aggatccctg ttccccttcg gagagggtct gtcctctgcg
     1021 ggaggtggtg tcgacgggac tgcccttcca ggaggagacc tcccagggca ggaacgacgc
     1081 ctactacagg agatactgtc acccggtctt cgaccgggac ggcagggtga cccacgtgat
     1141 tatgtacatt atcgacattt caacggacag aatgctcagg gagcagctca cccacgagac
     1201 cgcccgggca aacgagctga aggagcgggc agaggcggcg aacagggcga agagcgcctt
     1261 tctctccaac atgagccacg agatccgtac tcccctgaac gccgtcatcg gctttgccca
     1321 ccttctgaaa aactcgccgc tggacacagt ccagcgggac cacgccgaca gacttctcga
     1381 tgcctcccgc caccttctcc gacttgtcag cgacatcctc gacctttcca agatcgaggc
     1441 aaaccgcatg gaaccggaga tacgggattt cgagccttcc aggcacatcg accaggtatg
     1501 cgccctcgtg gcggaggacg cggccggaaa
//
LOCUS       DAOTHD010000604         1530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_97698_length_1530_cov_2.488814, whole genome shotgun sequence.
ACCESSION   DAOTHD010000604 DAOTHD010000000
VERSION     DAOTHD010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97698_length_1530_cov_2.488814"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..1247)
                     /locus_tag="P5219_09660"
     CDS             complement(117..1247)
                     /locus_tag="P5219_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27069.1"
                     /translation="MTKRWILFSAAFLCIISLFFWRRSPEIVSVALLFDGRDTTGAVV
                     SRQALQSVVLAMEHFNAFSSSWKFRPVLLSETDPSSALKAAAKKNVSAVVGGISVPFP
                     SLLSQASNRFGIPVISLAPGSFLAKGDDLVFRPRPESGGRTLGAEAKRRGMTAYSAIV
                     SGFESGYVQEFIRDFEAGAGAPPRRTMVFSGDLNKQIDDFERIAAGMDAMLLVLPDWL
                     AAIALRELRLRKPGIAVYASNRAVSHRTPLLTGDTGEGMVTAAALLRDWFPGGSGFPR
                     FVAETYGEHIPPITLSIGYDTVAMLGAALDRAGTTDPGAVARAFSGLERIESSAGPAS
                     LDAGGDLSLTQGTFSLTKEGWIPVTRPTASLTVSGVSNKAVP"
BASE COUNT          345 a          433 c          500 g          252 t
ORIGIN      
        1 ctgcagctgg gacctgatcc ggaagtaggc ggtgagccct ccctcgagga ggatgacggc
       61 caggaagaac aggaggatga ccgagaaaaa accgccgagg gagatgatcc tccgcttcat
      121 gggacagcct tgtttgaaac cccggagacg gtcagggagg cggtcggccg ggtaacggga
      181 atccagccct ctttcgtcag ggagaaggtt ccctgggtca gggacaggtc gccgcctgca
      241 tcgagggatg ccggtccggc tgacgattcg atacgctcca gtccggagaa ggcccgggcc
      301 acggccccgg gatccgtcgt tcctgcccgg tccagggcgg cgccgagcat ggctacggta
      361 tcgtacccga tggagagtgt gatgggcggg atgtgctctc cataggtttc agccacgaaa
      421 cgggggaaac cgcttccccc gggaaaccag tcccggagaa gggcggcggc ggtgaccatt
      481 ccttctccgg tatcccccgt gaggaggggg gtccggtggg atacggccct gttggaagca
      541 tacacggcga tgccgggttt tctcaggcgg agttccctca gcgctatggc ggcaagccag
      601 tcgggcagga cgaggagcat ggcgtccatg ccggcggcta tccgttcgaa atcgtcgatc
      661 tgcttgttca ggtcgccgct gaacaccatg gttctccggg gaggagcccc ggcccccgct
      721 tcgaagtcgc ggatgaattc ctgcacgtag ccgctctcga aaccgctcac gatggcggaa
      781 taggctgtca ttccccgccg cttcgcctcg gcgccgaggg tacggccgcc gctctccggc
      841 ctgggccgga agacaagatc gtcccccttg gcgaggaacg atccgggtgc aagggagatg
      901 acgggaatcc cgaagcggtt cgacgcctga gagagaaggg aagggaacgg aacggaaatt
      961 ccccccacca cggcggaaac attctttttg gcggctgctt tgagggcgga agagggatca
     1021 gtctccgaaa ggaggacggg ccggaatttc caggaagagg agaaggcatt aaagtgctcc
     1081 atggccagta caacggactg gagagcctgc cgggaaacca ccgcgccggt agtgtccctc
     1141 ccgtcgaaga gaagggccac ggaaacaatt tcgggcgacc tgcgccagaa aaaaagtgaa
     1201 attatgcaca aaaaagcagc ggaaaaaaga atccagcgct tcgtcactgc cccgcctccc
     1261 gggtggatgg tgcccctctc caggaaaaaa gtacaggatc catcgggagg gaccgctaca
     1321 gaccacggta ttttatcatt tcatgaaaca gcatttcaat caaaaggaaa aaagcacttt
     1381 gatttcagca gaaaaggacg gttatccgtc cagcgaatac ccttttttca aaaacaggga
     1441 gacgcagcag tccacgcagt cttcgtccag agcttctgcc cggaggcgga ggatcacgtc
     1501 cagggcggcg ccgatgccca gggcaggacg
//
LOCUS       DAOTHD010000605         1528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_97878_length_1528_cov_2.658520, whole genome shotgun sequence.
ACCESSION   DAOTHD010000605 DAOTHD010000000
VERSION     DAOTHD010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97878_length_1528_cov_2.658520"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="P5219_09665"
     CDS             complement(<1..263)
                     /locus_tag="P5219_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRX27070.1"
                     /translation="MVELTKENFDAEVKESALPVLVDFWGPKCGPCMALLPNVHKMAE
                     EYEGKVKFCSVDVSENRRVAIANKVMGLPTFLFWKDGAEVARI"
     gene            complement(263..703)
                     /locus_tag="P5219_09670"
     CDS             complement(263..703)
                     /locus_tag="P5219_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HRX27071.1"
                     /translation="MSCRRLLVTNNPLLRNAVPSCDFIDGNPLAVLLRSRDYVHLGWI
                     LLSHPLYGNLRPHQHPYRSILLERPDGEPRPTLDLQSLEYVENALGVYSAEKERILSD
                     KGMPEGVRDDFAVIDAELMKESLSRYGLWPRESLKTDLLKGGEG"
     regulatory      complement(801..908)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(1046..>1528)
                     /locus_tag="P5219_09675"
     CDS             complement(1046..>1528)
                     /locus_tag="P5219_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HRX27072.1"
                     /translation="PGLTFVTLPSVFAKMAGSSIWSALFFLLLFIAALTSAISLLEVV
                     AAYVIDKGWARPKAAVIMGLLIFALGVPSAMSLTGAPKIAGKDFLDAMDFLSSNVLLP
                     LGGVFIALFVGWFWTADARREVTNEGTHSFGMMEPWIWVCRVIAPLGILYIFITGLKW
                     "
BASE COUNT          350 a          426 c          455 g          297 t
ORIGIN      
        1 ctgatcctgg cgacttccgc tccgtccttc cagaagagga acgtgggaag acccatgacc
       61 ttgttcgcga tggcaactct ccggttctcg gaaacatcca cggaacagaa cttcaccttg
      121 ccttcgtact cctccgccat cttgtggaca ttggggagca gcgccatgca ggggccgcac
      181 ttggggcccc agaagtcaac aagcacgggg agcgcgctct ccttgacctc ggcgtcaaaa
      241 ttctctttcg tcaattcaac cattatcctt cacctccttt caatagatct gtcttcaaag
      301 attcccgggg ccacaggccg tatcgggaaa ggctttcctt catgagctcg gcgtcgatga
      361 cggcaaaatc gtcccggaca ccttcgggca ttcccttgtc tgagagaatc cgctccttct
      421 cggcggaata caccccgaga gcgttttcga cgtattccag ggactggagg tcgagagtcg
      481 gccggggttc accgtcaggg cgctccagga gaatggagcg gtacggatgc tggtggggac
      541 ggaggttccc gtagagggga tgggacagca gtatccatcc gagatggaca tagtcccttg
      601 accgcagaag aacagcaaga gggtttccgt cgatgaaatc gcaggacggg acagcgttcc
      661 gcaaaagggg attgttggtt acaagaagcc tgcgacagga cattcaactg cctccctata
      721 cctataggta agacactctc tgtcgcatag ccccggagag gagagaaacc gcatcggggg
      781 agctattcat ttcagagatc cgtccggccg caatcctttc gcctgagagt ttccgcccgc
      841 cctgtacggc gggccttgcc ccttcggcgc tctcctgtgt gctggaaagt ctctcttgcg
      901 gcgttcatcc ggagatcatg tttattttac acaatgaaaa aaaggagtca agcctttttg
      961 tgaacggaac aacaaaatga ttttcggggc cggcccggga ataaaaagag ggcccccgaa
     1021 ggggccctca aaaaagctgg tgccgtcacc acttgaggcc ggtgatgaag atgtacagta
     1081 ttccgagagg agcgatgacc ctgcagaccc agatccacgg ctccatcatg ccgaaggagt
     1141 gggtaccctc gttggtgacc tctctccttg catccgccgt ccagaaccag cccacaaaga
     1201 gggcgatgaa cacgccgccc aggggcagaa ggacgttgga tgacaggaag tccatggcgt
     1261 cgaggaagtc tttccccgcg atcttcggcg ctccggtgag ggacatggcg gaaggcacgc
     1321 caagggcgaa gatcaggagt cccatgatga ctgcggcctt gggccgtgcc catcccttgt
     1381 cgatgacgta ggccgccacc acttcgagaa gagagatggc cgatgtcagg gcggcgatga
     1441 agagaagcag gaagaacagg gccgaccaga tggaagatcc cgccatcttg gcgaagacgg
     1501 agggaagggt gacgaaggtc agtccggg
//
LOCUS       DAOTHD010000606         1526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_98030_length_1526_cov_3.707682, whole genome shotgun sequence.
ACCESSION   DAOTHD010000606 DAOTHD010000000
VERSION     DAOTHD010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98030_length_1526_cov_3.707682"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="P5219_09680"
     CDS             <1..207
                     /locus_tag="P5219_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012009788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HRX27073.1"
                     /translation="DRVLVGANAVILEGVRVGAGAVVAAGAMVTKDVPPRTVVAGSPA
                     RVVKDVDEKTESKTEIVQDLRNLK"
     gene            221..1426
                     /locus_tag="P5219_09685"
     CDS             221..1426
                     /locus_tag="P5219_09685"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRX27074.1"
                     /translation="MIVQKYGGSSVATAEKIRGVAERIRQRVDGGEKLVVVVSAMGKT
                     TNSLIALAEQVSPEPSPRELDMLLATGEQVTSALLAMALCDMGIPALSYNAFQLGLTT
                     TGSFNNARIMDLNLSRLRMQLERRSVLVVTGFQGITPEGDVTTLGRGGSDTSAVAIAA
                     KCGCPCEIYSDVPGVFTCDPNRVPGARKLDYITYDEMLELSSLGAKVLHSRAVEIAKK
                     YSVDLYCGSTFSDERGTCVVNSLPEWLEQPVVSGVTADSNQMRVNISRLPRSMEALSA
                     LFTGLAEQGVNVDMISTVNENGHSHLTFTVVDATEKAVLRTVRDVLAPWEGWIVSEDR
                     NVVKVSAVGVGMKSAPGVAARFISALSRKGIAVLGTTTSEIKISALVAREDGDMALRA
                     LVEEFGLGE"
BASE COUNT          281 a          441 c          534 g          270 t
ORIGIN      
        1 gacagggtcc tcgttggggc caacgccgtc atcctcgaag gcgtcagggt cggcgccggg
       61 gcggtggtgg cggcgggcgc catggtgacg aaggacgttc ctcccaggac ggtggtggcg
      121 ggttctcccg ccagggtggt caaggacgtg gacgaaaaga cggaatcgaa gacggagatt
      181 gtgcaggatc tccgaaacct gaagtaggag aggaccggac gtgatcgtac agaagtatgg
      241 cgggtcgtcg gtggccacgg cggagaagat ccggggcgtg gccgagagaa ttcggcagag
      301 ggtggacggg ggagagaagc ttgtggtggt ggtctccgcc atggggaaaa ccaccaactc
      361 cctcatagct cttgcggagc aggtttcgcc ggagccgtcc cccagggagc tggacatgct
      421 ccttgccacg ggggagcagg tcacgtccgc cctcctcgcc atggcgctgt gcgacatggg
      481 gataccggcc ctctcctaca acgctttcca gctcggcctg accaccacgg gcagcttcaa
      541 caacgcccgg atcatggacc tgaacctctc caggctcagg atgcagctcg agcggcggag
      601 cgtcctcgtg gttaccgggt tccagggcat taccccggaa ggggacgtta ccaccctggg
      661 caggggaggc tcggacacgt cggcggtggc catcgccgcc aagtgcggct gtccgtgcga
      721 gatctacagc gacgtgccgg gggtgttcac ctgcgacccg aacagggttc ccggagcccg
      781 gaagctcgac tacatcacct acgacgaaat gctggaactg tcgtccctgg gagccaaggt
      841 gctccattcc agggctgtgg aaatcgcgaa aaaatactcg gtggacctct actgcggctc
      901 caccttctcc gatgaaaggg ggacctgcgt agtgaattcc cttcccgagt ggctcgagca
      961 gcccgtcgtt tcgggcgtga ccgccgacag caaccagatg cgggtgaaca tctcccgcct
     1021 tccccggtcc atggaggccc tgagcgccct cttcacgggg ctggcggagc agggagtgaa
     1081 cgtggacatg atctccacgg tgaacgagaa cggccattcc cacctcacct tcaccgtggt
     1141 ggacgctacg gaaaaggcgg tgctcaggac agtgagggac gtccttgctc cctgggaggg
     1201 atggatcgtg tccgaggacc ggaacgtggt gaaggtctcc gccgtgggag tggggatgaa
     1261 gtcggccccg ggggtggccg cgcggttcat ctccgccctg agccgcaagg ggattgccgt
     1321 gcttggaacc accacgtcgg agatcaagat ttccgccctt gtggccaggg aagacggcga
     1381 catggctctc cgggccctgg tggaagagtt cgggctgggg gaatgacatt catgctgtct
     1441 cccgtctatt ctccctgggg tcttgcagtg gagcgtgccg agggaatgaa ggtctatacg
     1501 gacaggggcg tctttctgga tgcctt
//
LOCUS       DAOTHD010000607         1526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_98036_length_1526_cov_3.426241, whole genome shotgun sequence.
ACCESSION   DAOTHD010000607 DAOTHD010000000
VERSION     DAOTHD010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98036_length_1526_cov_3.426241"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="P5219_09690"
     CDS             complement(<1..339)
                     /locus_tag="P5219_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202360.1"
                     /note="catalyzes the interconversion of 3-phosphoglycerate
                     and 2-phosphoglycerate; this enzyme does not require the
                     cofactor 2,3-bisphosphoglycerate as a phosphate donor;
                     BPG-independent PGAM; aPGAM; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HRX27075.1"
                     /translation="MKRMELLRRLARDNGKKMILLVMDGIGGLPGPDGKTELEAARTP
                     NLDALAEKSELGLLDMVDSGITPGSGPGHLSLFGYDPLEFSIGRGILEAMGVGALVGP
                     GDVCARGNFCT"
     gene            477..>1526
                     /locus_tag="P5219_09695"
     CDS             477..>1526
                     /locus_tag="P5219_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRX27076.1"
                     /translation="MKFSERVRALQSSPVRRLLPYAEEARAKGKKIYPLNIGQPDIKT
                     PPSFLKAIRDFSQDVIAYATSQGNNDLRDAMSGYYKNFNIPLEREDIIVTNGGSEALM
                     FAFMAMCDPGDEVLIPEPFYANYNAFAGSVNVKVRPVTTKAEEGFHLPPREEIEALVT
                     PKTRAILVANPGNPTGTIYTRDEMDMLASLALKHELFIVADEVYREFVYDGLQYTSFG
                     HLPEIADRVIIVDSVSKRYSACGARIGCIACRNRDFMAQVLKIAQGRLCVPTLEQVGA
                     TALYSTPKSYLEEVNKEYQSRRNTLYKALKEMPGVVCAEPKGAFYVMVKLPVDDAEKF
                     IIWMLQNFDVNGETVM"
BASE COUNT          349 a          464 c          397 g          316 t
ORIGIN      
        1 ggtgcagaaa tttcccctgg cgcagacgtc acccgggccc accagagctc cgacgcccat
       61 ggcctcgaga atgcccctcc cgatggagaa ctcgagagga tcgtagccga acagtgaaag
      121 atggcccggg ccgcttcccg gagttattcc cgaatccacc atgtcgagga gaccgagttc
      181 gctcttttcc gccagagcat cgaggttcgg cgtacgggcg gcttcaagct cggtttttcc
      241 gtccggcccc gggagtccgc cgatgccgtc catgacgagc aggatcattt ttttaccgtt
      301 gtcgcgggca agtctccgca gaagttccat tcttttcatg tgtctgattc cacctccggg
      361 taatttccag gaacaaattt gtagtaagta ctcgtttttt tctgcaaaaa ccactgcatt
      421 ttatgatatc attttctcca aaggtccatc cagtttacat caagggggta atacaggtga
      481 agttttccga acgagttcgt gcgcttcagt cgtcgcctgt ccgccgtctt cttccctatg
      541 cggaggaagc ccgggcgaaa ggcaaaaaga tctatcccct caatatcggc cagccggaca
      601 tcaagactcc tccatcgttc ctcaaggcca tacgggactt ttcccaggac gtgatcgcct
      661 acgccacctc ccagggaaac aacgatctcc gtgacgccat gagcggctac tacaaaaact
      721 tcaacattcc cctcgaacgg gaggacatca tcgtcacaaa cggcggaagc gaagccctca
      781 tgttcgcctt catggccatg tgcgatcccg gggacgaagt gctcatcccc gagcccttct
      841 acgccaatta taacgccttt gccggttcag tgaacgtaaa agtgcgcccc gtcaccacaa
      901 aagccgagga agggttccat cttcccccca gagaagaaat cgaagctctc gtcactccga
      961 agaccagggc gatcctcgtg gcaaatcccg gcaaccccac cggaaccatc tatacgagag
     1021 acgaaatgga catgcttgcc tcccttgccc tgaagcacga gcttttcatc gtcgccgacg
     1081 aggtatacag ggagttcgtc tatgacggac tgcagtacac gagcttcggc catctccccg
     1141 agatcgccga ccgagtcatc atcgtggact ccgtgtccaa gcgctacagc gcctgcggag
     1201 cccgtatcgg atgcatcgcc tgccggaaca gggacttcat ggcccaggtt ctgaagatcg
     1261 ctcagggaag actgtgcgtt cccaccctgg agcaggtcgg cgctaccgcc ctgtacagca
     1321 cgccgaagtc ctacctcgag gaagtaaaca aggagtacca gtccaggcgg aataccctgt
     1381 acaaggcgct gaaggagatg cccggagtgg tgtgcgccga gcccaaggga gccttctacg
     1441 tgatggtgaa gctgcctgtg gacgatgccg agaagttcat catctggatg ctccagaact
     1501 tcgacgtaaa cggtgaaacc gtaatg
//
LOCUS       DAOTHD010000608         1524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_98270_length_1524_cov_2.309054, whole genome shotgun sequence.
ACCESSION   DAOTHD010000608 DAOTHD010000000
VERSION     DAOTHD010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98270_length_1524_cov_2.309054"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="P5219_09700"
     CDS             <1..187
                     /locus_tag="P5219_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRX27077.1"
                     /translation="QLASLENDIQMSRRYYNGAARDYNISIATFPAVLIAQKFGYEKA
                     DYFEAAEEEKTAPKVSF"
     gene            217..>1524
                     /locus_tag="P5219_09705"
     CDS             217..>1524
                     /locus_tag="P5219_09705"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HRX27078.1"
                     /translation="MVLFRKLAPALLALALVLLPRPSGAEESILLFSSSASVRGDASL
                     EVREDITVRVEGVRIRKGIFRDFPTTYTDSSGKTVRVGFSVEEALLNGKRVPYSTESR
                     SNGVRVYLGDPKGNAPLGEQTYTLVYVTTGQLGFFDEHDELYWNVTGNDWVFPIMKAR
                     FSASLPGNVPFSSVDIYTGPQGARGQDARILPDNSVETTRRLAPGEGLTVAYTWPKGI
                     VEKPKPPIRYALFDRFGTVFFWSVPLLLLLYYALAWIRWGKDPPRKPVIPLFSPTAGN
                     GPGFLRFVRRMGMDNSCFTAEILNLAVRGFLKIEEMSLEEAMERQGEIGKKSLLRGVV
                     SLASKLAGKRYALVSLPREGWKTTPSKEESRLFNTLFGGGRKELHLVQDNHEILGKAK
                     ETLEASWSDASKSLFSKNTLIWLAGFLIPVLIYALLAWGGQEDG"
BASE COUNT          306 a          503 c          415 g          300 t
ORIGIN      
        1 gcagctcgcc tccctcgaga acgacatcca gatgtccagg cgctactaca acggcgccgc
       61 gagggactac aacatctcca tcgccacctt cccggcagtg ctgatcgccc agaagttcgg
      121 ctacgaaaag gccgattact tcgaggctgc ggaggaagag aagaccgccc cgaaagtctc
      181 gttctaggct ccaccgaaga cggaggaggc gcatccatgg tgttgttccg caaacttgcg
      241 ccggcgcttc tcgcgctggc cctggtgctc ctcccccggc cttccggggc tgaggagtcc
      301 atacttcttt tttcctcttc ggcttccgtc cgcggggacg cttccctcga agtacgggaa
      361 gacatcaccg tccgcgtgga aggagtgcgt atccggaaag gcattttccg ggactttccc
      421 accacctaca cggacagttc cggcaaaacc gtccgggtcg gattttccgt tgaagaagcc
      481 cttctcaacg gaaaacgggt tccctacagc accgaatccc gctccaacgg cgtccgggtt
      541 tacctcggag acccgaaagg aaatgccccc ctcggcgagc agacttacac tctcgtctac
      601 gtgaccaccg gccagctcgg ttttttcgat gaacacgacg aactctactg gaacgtcacc
      661 ggaaacgact gggtcttccc cataatgaaa gcccgctttt ccgcctcgct gccgggcaat
      721 gtcccctttt cgtccgtcga tatctataca ggcccccagg gagcccgtgg acaggatgcc
      781 cgcatcctcc ccgacaactc ggtggagacc accaggcgtc ttgcccccgg tgaaggcctg
      841 accgtggcct atacctggcc caagggcatc gtggaaaagc cgaaaccgcc catccgctat
      901 gccctcttcg accgcttcgg cactgtattt ttctggagcg ttccccttct gctgctcctg
      961 tactacgccc tcgcctggat ccgctggggg aaggatcccc cgagaaaacc ggtgatcccc
     1021 ctcttcagcc ccacggcagg caacggaccg ggcttcctcc gcttcgtcag gcggatgggc
     1081 atggacaact cctgcttcac cgctgaaatc ctgaacctgg cggtcagggg cttcctcaaa
     1141 atcgaggaga tgtccctcga ggaggccatg gagcggcagg gcgaaatagg gaaaaagagc
     1201 ctgctgcggg gcgtggtgtc cctggcttcc aagcttgccg ggaaacggta cgcccttgtg
     1261 tcccttccca gggaaggatg gaagacaact ccctcgaagg aggaatcacg gcttttcaac
     1321 actcttttcg gcggaggaag aaaagagctc catctcgtcc aggacaacca tgaaatcctc
     1381 ggcaaggcga aggaaaccct ggaagcctcc tggtcggacg cctccaaatc tcttttctcg
     1441 aagaacaccc ttatctggtt ggcgggattc ctcatccccg tcctgatcta cgcccttctt
     1501 gcctggggcg gccaggagga cggc
//
LOCUS       DAOTHD010000609         1523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_98376_length_1523_cov_2.184605, whole genome shotgun sequence.
ACCESSION   DAOTHD010000609 DAOTHD010000000
VERSION     DAOTHD010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98376_length_1523_cov_2.184605"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="P5219_09710"
     CDS             <1..810
                     /locus_tag="P5219_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27079.1"
                     /translation="PESFSAALDELFEVFPFLVEVLAASPAIAFDTPMDEMEYQMQEV
                     GKQLNKEFGAVMDGFSRAAQTVQNTASSIGRGLKNAVGWVADKVAAGHHKIGSVVGQK
                     NWATAMAGTKFVTAITLAGVGLVVVISAPAVSTAGALTAVAVHTAANVGACVSFANDM
                     SQIHGGRGAGDVETATTRINQATSLIGVVSGDPKEITLAVLGATGDEIANTTGRRTLS
                     PEELAGYLNAPDRQAFLESLNARRDYQSPADMGSGGGEGGNGGSGGGCSCP"
     gene            complement(859..>1523)
                     /locus_tag="P5219_09715"
     CDS             complement(859..>1523)
                     /locus_tag="P5219_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX27080.1"
                     /translation="SQLSGGQQQRVALARAVVMEPSLLLFDEPLSNLDAKLREQMRID
                     FRKLQQQFGITSIYVTHDQIEAMSISDRVVVMNRGKIEQTGAPRELYSKPANRFVASF
                     IGKAAFLPGEKTAAGWTLLGRPWNPEAGGYLPQGKYDVMIRPEGIRLSPAGSGLGHLK
                     GTVIRSTYLGSLMEYEVDLPGTEPLTVHIPNPFERNPLAPGAAAELTLIPEGLHFLPA
                     EK"
BASE COUNT          298 a          423 c          490 g          312 t
ORIGIN      
        1 ccggagagtt tttcggcagc cctggacgag ctttttgaag tcttcccgtt cctcgtggag
       61 gtgctcgccg cttctccggc aattgctttt gataccccga tggacgagat ggaataccag
      121 atgcaggagg taggaaaaca gctgaacaag gagttcggtg ccgttatgga cggtttttcc
      181 agggcggcgc agacggtcca gaatacggct tccagcatcg gcagaggact gaagaacgcc
      241 gtgggatggg tcgctgacaa agtcgccgca ggacatcaca aaatcggcag cgttgtcgga
      301 cagaagaact gggcgacggc catggcggga acgaagttcg tcacggcgat tacccttgcg
      361 ggtgtaggtc ttgttgtagt catttcagcc cccgccgtat cgactgcagg agccctgacc
      421 gccgtggctg tccacacggc cgcgaatgtc ggggcctgtg tttcctttgc caacgacatg
      481 agccagatcc atgggggaag aggcgcgggg gatgtcgaaa cggcgacgac ccgaataaac
      541 caggctacgt ccctgatcgg agtggtgagc ggtgacccga aggaaatcac cctggccgtt
      601 ctcggagcca ccggggacga gattgccaat acgacaggga gaagaaccct ttcaccggag
      661 gaactggccg ggtacctgaa cgcccccgac aggcaggctt tcctggaaag cctgaacgca
      721 cggagagatt accagtctcc tgcagatatg ggaagcggcg gaggagaggg aggcaacggg
      781 ggcagcggag gcggatgcag ctgtccgtaa aagggaatgt tttccgggga gagccttatg
      841 aaaggctctc ccctttttct atttttccgc cggaagaaaa tggaggcctt ccggtatcag
      901 cgtgagctcc gccgcggccc ccggagcgag gggattccgt tcaaaagggt ttgggatatg
      961 tactgtgagc ggttccgtgc cggggaggtc cacttcgtat tccatgaggc tgccgaggta
     1021 ggtggaccgg ataaccgttc ccttgaggtg tcccagcccg cttcctgcgg gagagagccg
     1081 gattccctcc gggcggatca tgacgtcgta ttttccctga ggcaggtatc ctcccgcttc
     1141 cggattccac ggccttccga gaagagtcca tcctgccgcc gtcttttctc ccgggaggaa
     1201 ggcggccttc ccgatgaagg aggccacgaa gcggttcgcc ggttttgagt acagctccct
     1261 tggggctccc gtctgttcga tttttccccg gttcatcacc accaccctgt cggagatgct
     1321 catggcctcg atctggtcgt gggtgacgta gatgctggtg atcccgaact gttgctggag
     1381 cttccggaag tcgatcctca tctgctccct gagcttggcg tccagattcg acaggggttc
     1441 gtcaaagagg agcagggaag gttccatcac caccgccctt gcaagggcga cccgctgctg
     1501 ctgtcccccc gagagctggc ttg
//
LOCUS       DAOTHD010000610         1518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_98801_length_1518_cov_3.084757, whole genome shotgun sequence.
ACCESSION   DAOTHD010000610 DAOTHD010000000
VERSION     DAOTHD010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98801_length_1518_cov_3.084757"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="P5219_09720"
     CDS             complement(<1..267)
                     /locus_tag="P5219_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27081.1"
                     /translation="MNRSDRRMAVVVLSAVLLFFPAVPAAAFVLAAPLEGTVGIAMER
                     FAGDVLERADREQATLVVFRLDTPGGLVSSMRGITTAILESKVPV"
     gene            complement(264..1508)
                     /gene="radA"
                     /locus_tag="P5219_09725"
     CDS             complement(264..1508)
                     /gene="radA"
                     /locus_tag="P5219_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRX27082.1"
                     /translation="MEKEPDAPVPGASLRSSRASRVDVASVVPPERTPTGLDEFDRVL
                     GGGWVPGGVFLLGGKPGIGKSTLLLQTCGLMASAGHRVLYVSGEESESQVALRARRLG
                     SASAGLDLFCDSDLSAALSCLDGHRFFVVDSVQAMRADGAEGWAGSPGQVRASAQMCI
                     AAAKERSIPAVLVGHITKEGRIAGPMLLEHMVDGVLTFSGDDYSPYRMLRASKNRFGS
                     TDELGVFEMGENGLYPVKDVSGLYWNKAEKSVPGVAMTVVLEGTQPLVAEIQSLAAAT
                     AFPYPKRTGRGIDLNKIQLFTAVLEKRCGTGCGLFDIYVNVAGGLALREPGADLALCA
                     ALASAVRDVPLPEKCVFLGEVGLAGEVRPVVRLARRLHEAARLGFTRAVVSSREEPAG
                     KKAPIDIVRAATLNDALKAVMK"
BASE COUNT          312 a          542 c          412 g          252 t
ORIGIN      
        1 caccggaacc ttcgattcga gtatcgccgt ggtgattccc ctcatggaac tcaccagccc
       61 gcccggggtg tccagccgga agaccaccag cgtcgcctgc tcccggtcag ccctttccag
      121 gacatccccg gcgaaacgtt ccatggctat ccccacagtc ccctcaaggg gagcagcaag
      181 aacaaaagcc gccgcaggga cagccggaaa aaacagcagg accgccgaaa gaacaacgac
      241 cgccatccgc cgatcacttc ggttcatttc atcaccgcct tcagggcatc attcagtgtc
      301 gccgccctca caatgtctat gggggctttc ttccccgccg gttcctccct ggagctgacc
      361 accgccctgg taaatcccag ccgggccgct tcatggagcc ttctggcaag ccgtaccacc
      421 ggccggactt ctcccgccag acctacctcc ccgaggaaaa cgcacttttc agggagggga
      481 acatcccgta ccgccgaagc gagagccgcg cagagcgcaa ggtccgcccc gggctcccgg
      541 agagcgagac ctcccgccac gttcacataa atgtcgaaaa gaccgcatcc tgttccgcac
      601 cgcttttcca gcacggcggt gaagagctgg atcttgttca ggtcgattcc cctgccggtc
      661 ctcttcggat agggaaaagc cgttgcggcg gccaaagact gtatttccgc cacaagaggc
      721 tgggtaccct cgagaaccac tgtcatggcc acaccgggaa ccgacttctc ggccttgttc
      781 cagtacaggc cgctcacgtc cttcaccgga taaaggccgt tttcccccat ttcaaagaca
      841 ccgagctcgt cggtgctgcc gaaacggttc ttgctcgccc ggagcatccg gtatggagaa
      901 tagtcgtccc ccgagaaggt cagcacgccg tccaccatgt gctccagcag catggggccc
      961 gcaattctgc cttccttggt tatatgcccc accaggactg cggggatact ccgctccttg
     1021 gcggcggcga tgcacatctg ggccgacgcc cggacctggc cggggctgcc tgcccatcct
     1081 tccgctccgt cggccctcat ggcctgtacg ctgtccacca caaaaaaacg gtggccgtcc
     1141 agacatgaaa gggcggcaga cagatcgctg tcacagaaaa gatccaggcc ggccgaggcc
     1201 gaacccagcc tgcgggccct cagggccacc tgggattccg actcttcgcc ggaaacatag
     1261 agaaccctgt gtcccgccga ggccatcaaa ccgcaggtct gcaaaagcag ggtggacttc
     1321 ccgataccgg gttttccccc gaggagaaaa acacctccgg gaacccatcc tccgcccaga
     1381 acccggtcga actcatccag gcccgtgggc gtcctttccg ggggaaccac cgaggcaacg
     1441 tcaaccctgg acgcccggga cgaacgaagg gacgccccgg gaacgggagc gtcaggttcc
     1501 ttctccaggg ccccccac
//
LOCUS       DAOTHD010000611         1513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_99282_length_1513_cov_2.342936, whole genome shotgun sequence.
ACCESSION   DAOTHD010000611 DAOTHD010000000
VERSION     DAOTHD010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99282_length_1513_cov_2.342936"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="P5219_09730"
     CDS             <1..1165
                     /locus_tag="P5219_09730"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRX27083.1"
                     /translation="LEKVEATGKYEVTLTFKAPFAPWKNLLAFMNGGPVIYPKDVASG
                     AEKTPITPEQYIGTGPYKFAERNPGRYIKLVRNEEYSARTEAADGYAGKREALFDEIR
                     FIPVPDVGTRVNGVKAGDYDYAEQILGDLFDSLKEDPSVVTTVNQGANQGMMFFNSRA
                     GVLKDNSKLRRALQAAVNMEPVLQSAIGPAELWKASGSFMPESTLWYSKAGLDRFSAN
                     NPELAKKLAKEAGYNGEKITLMASTSYKFHYDSTLVIVKQLQAAGFNIDHQIYDWATL
                     ISKRGDPAQWDIFFTHHGFVPDPILYTFMSESYPGWWTTETKRALAAEFAGTLDAAKR
                     KAVWDRIQALVYEEVPVMKTGDHFTYDIYSPKVQGLSASSLIWPKFWGVSFGK"
     gene            1229..>1513
                     /locus_tag="P5219_09735"
     CDS             1229..>1513
                     /locus_tag="P5219_09735"
                     /inference="COORDINATES: protein motif:HMM:NF040248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRX27084.1"
                     /translation="MFSYIVRRIGSMIPSLLLASIIVFSFIHLIPGDPAQIMLGDQAT
                     PEQVQGLREAMGLDRPLYVQYGRWLSGVVKGDFGTSIFFHQPVLHVILDRA"
BASE COUNT          336 a          460 c          421 g          296 t
ORIGIN      
        1 tctggaaaag gtggaagcca cgggcaagta cgaagtgacc cttaccttca aggctccctt
       61 cgctccgtgg aagaacctcc tggccttcat gaacggcggt cccgtgatct acccgaagga
      121 tgtggcgtcc ggcgccgaga aaacgccgat cacccccgag cagtacatcg gcacggggcc
      181 ctacaaattt gccgagagga acccggggcg ctacatcaag ctggtccgca acgaagaata
      241 ttccgccagg acagaggccg ccgacggtta tgccggcaag cgggaagccc tctttgacga
      301 aatccgcttc attcccgtcc ccgacgtggg aacgcgggtc aacggcgtca aggctggaga
      361 ttacgattat gccgagcaga tcctgggcga cctcttcgac tccctgaaag aagatccttc
      421 ggtggtcacc accgtcaacc agggcgcaaa ccaggggatg atgttcttca actcaagagc
      481 gggagtgctg aaggataatt ccaagctccg ccgggccctc caggctgccg tgaacatgga
      541 gccggtgctc cagtcggcca tcggtcctgc ggaactgtgg aaagcttccg gctcctttat
      601 gcccgagagc actctctggt attcgaaggc cgggctcgac aggttcagcg ccaacaatcc
      661 cgaactcgca aaaaaactcg cgaaagaggc ggggtacaac ggcgagaaga ttaccctcat
      721 ggcctccacg tcctacaagt tccattacga cagcactctc gtgatcgtga agcagctcca
      781 ggcggcaggc ttcaacatcg accaccagat ctacgactgg gcgaccctca tctccaagcg
      841 cggcgacccg gcacagtggg atattttctt cacccaccac ggttttgttc cagatcccat
      901 cctgtataca tttatgagcg agtcctaccc agggtggtgg acaacggaaa cgaagagggc
      961 ccttgccgcc gaattcgccg gtaccctgga cgcggcaaag cgcaaggcag tatgggacag
     1021 gatccaggca cttgtctacg aagaagtacc cgttatgaag acgggagatc actttaccta
     1081 cgacatctac agcccgaagg ttcagggact ctccgcttca tccctcatct ggcccaaatt
     1141 ctggggcgtt tcgtttggga agtagcctga aaacaggggg agggacagtt ccctccccct
     1201 cagcatccga cgaaaggggg gagcctccgt gttttcttat atcgtcaggc gaatcgggag
     1261 catgattccg tctctccttc tcgcttcgat catcgtgttt tcattcatcc acctgattcc
     1321 gggagatccc gcccagatca tgctgggaga ccaggcgacg ccggaacagg tccaggggct
     1381 ccgggaagcc atgggcctcg accgcccttt atacgtgcag tacggccgct ggctctctgg
     1441 agtggtgaag ggagatttcg gcacatccat atttttccac cagcctgtgc tccacgtcat
     1501 cctcgaccgg gct
//
LOCUS       DAOTHD010000612         1509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_99644_length_1509_cov_2.881706, whole genome shotgun sequence.
ACCESSION   DAOTHD010000612 DAOTHD010000000
VERSION     DAOTHD010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99644_length_1509_cov_2.881706"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="P5219_09740"
     CDS             <1..1057
                     /locus_tag="P5219_09740"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRX27085.1"
                     /translation="SAKRGADYAAEHGIATAGTSVEEAVSDPAVDAVYISTTNEKHFD
                     QAMAAIAAGKHVISEKPFTGYFGVPGDVQPVGKVCKRKMYDAVLEGMERTAGIVRKSG
                     RLFCYAEDWVYAPAVAKASEILKKTGDKILFLKAEESHSGSHAWHAAEWAQTGGGSMI
                     RQGCHPLSAVLHLKMTEAKARGEAFSVESVVCDTGVVTERLTKEERRFIAANPVDVED
                     WSMLSLTFGDGTKATIFSGDFVTGGVRNLIEFNTNTGTLVCNIAPNNGMMTYAADASV
                     LKDVYLTEKVETTAGWQYVLLDEESARGYLQEAQDFMECVATGRQPLSGLDLAYETIK
                     ITYAGYLSAEKGERVKL"
     gene            1077..>1509
                     /locus_tag="P5219_09745"
     CDS             1077..>1509
                     /locus_tag="P5219_09745"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HRX27086.1"
                     /translation="MNILVFDTPDELAAAAAKRGAELLRKTVEEKGRARLMLSTGASQ
                     FGTLRELVSHPVDWSKVEMFHLDEYVGLPESHGASFRRYLQERFVSKVNPGKVHFVNW
                     EGGAEQNMKALSEEISKAPVDVAFIGIGENGHIAFNDPPADF"
BASE COUNT          314 a          491 c          479 g          225 t
ORIGIN      
        1 ctcggcaaag cgtggtgccg actatgcggc cgagcatggc atcgcgaccg ctggcaccag
       61 cgtcgaggag gcggtgtccg atccggccgt cgacgccgtc tacatctcca ccaccaacga
      121 gaagcatttc gatcaggcga tggcagcgat cgccgccggc aagcacgtga tctccgaaaa
      181 gcccttcacc ggctacttcg gcgttccggg ggacgtgcag cccgtgggga aggtttgcaa
      241 gcggaagatg tacgacgccg tcctcgaggg aatggagcgg accgccggca tcgtccggaa
      301 aagcggcagg ctcttctgct acgccgagga ctgggtctac gcccccgccg tggccaaggc
      361 ctcggaaata ctgaagaaaa cgggggacaa gatcctcttc ctcaaagccg aggaaagcca
      421 cagcggctcc cacgcctggc acgccgccga gtgggcccag accggcggag gctccatgat
      481 ccgccagggg tgccaccccc tgtcggcggt ccttcacctc aagatgacgg aagcgaaggc
      541 caggggagaa gccttctccg tggagagcgt ggtgtgcgac accggcgtgg tcacggaaag
      601 gctcacaaag gaagagcgga ggttcatcgc cgccaatccc gtggacgtgg aggactggtc
      661 catgctctcc ctcaccttcg gcgacggcac caaggccacc atcttctcgg gcgacttcgt
      721 caccgggggc gtgcggaacc tcatcgagtt caacaccaac acgggaaccc tggtctgcaa
      781 catcgccccc aacaacggca tgatgaccta cgccgccgac gcctccgtgc tgaaggacgt
      841 ctacctcacc gaaaaggtgg agaccaccgc gggctggcag tacgtcctgc tggacgaaga
      901 atccgcccgg gggtacctcc aggaggccca ggacttcatg gagtgcgtgg ccacgggcag
      961 gcagcccctc tccgggctcg acctcgccta cgaaaccata aagatcacct acgcgggcta
     1021 cctctccgcc gaaaaaggcg agagggtgaa gctctagcgc cgccggggga gaaaaaatga
     1081 acattctcgt tttcgacacg cccgacgaac ttgccgccgc ggcggcgaag cggggcgccg
     1141 aactgctccg gaaaaccgtg gaggaaaagg gccgggcccg gctgatgctc tccacgggag
     1201 cgtcccagtt cggcaccctc cgggagcttg tctcccaccc cgtggactgg tcaaaggtgg
     1261 agatgttcca cctggacgag tacgtgggcc tgcccgaaag ccatggggca agcttccgcc
     1321 gttaccttca ggagcggttc gtctcgaagg tcaaccccgg gaaggtccat ttcgtcaact
     1381 gggagggcgg cgcggaacag aacatgaagg ccctgtcgga ggaaatatcc aaagcccccg
     1441 tggacgtggc cttcatcggc atcggggaga acggccacat cgccttcaac gacccccccg
     1501 ccgattttg
//
LOCUS       DAOTHD010000613         1507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_99852_length_1507_cov_2.626033, whole genome shotgun sequence.
ACCESSION   DAOTHD010000613 DAOTHD010000000
VERSION     DAOTHD010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99852_length_1507_cov_2.626033"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1485)
                     /locus_tag="P5219_09750"
     CDS             complement(<1..1485)
                     /locus_tag="P5219_09750"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HRX27087.1"
                     /translation="MENPLPDSVSELQSLVAEVHHKLVGTEREKDRVEHENNILREQI
                     RLLRHQLYGRKSEKLTSPEEMGRLLFPELFEQQDTTQETPQEEVEVKPHTRAKTGRKP
                     FPEDLPRHRVEHDIPEEEKVCGCGARMDCIGEESSEQLDIVPATVQVIQHVRFKYACS
                     HCHGEQSDEKAVKIAPPPAQILPKCMVSPGMLAHIMTGKFTDALPFHRQENMLSRYGV
                     EVSRASMCNWAMALYERCLPLAELLELSALDGEMLGIDETTVSFLGGKKDKKQKANSA
                     YMWVIRGGPPEKPVVSFHFAASRAQSVPMELLSGYQGIVMSDGYAGYKFLDQDKRIIH
                     AGCWAHARRLFHDVNKGLKKGHSDRYADHALKVIKELYAIESEVKGQAPELIQAARQE
                     KAKPVLDAFERWLNEHGHRFAPKTNLGKAFGYSLSQLPKLLRYLEDGRIPIDNNPTEN
                     AIRPFVVGRKNWLFVNSLKGARASAFFYSLIETAKANGLEPFSYL"
BASE COUNT          272 a          444 c          457 g          334 t
ORIGIN      
        1 caggtagctg aagggctcaa ggccgttggc cttggcggtt tcgatgaggc tgtaaaagaa
       61 ggcgctggcc ctggccccct tgaggctgtt gacgaagagc cagttcttgc gcccgaccac
      121 gaagggcctg atggcgtttt cggttgggtt gttgtcgatg gggatgcggc catcctccag
      181 gtagcgcaac agtttgggca gctgggagag gctgtagcca aacgccttgc ccaggtttgt
      241 cttgggagcg aaccggtgcc cgtgctcatt cagccagcgc tcgaacgcat ccaggacagg
      301 cttggccttt tcctgccgtg ccgcctgaat aagctccgga gcctggccct tgacttcgct
      361 ttctatggcg tagagttcct tgatgacctt gagggcatgg tctgcataac gatcggagtg
      421 ccccttcttc agacctttgt tcacgtcgtg gaacaggcgc cgggcatgcg cccagcaccc
      481 tgcatggatg atgcgcttgt cctggtccag gaacttgtag ccggcatatc catcgctcat
      541 gacgatgccc tggtatcccg acaaaagttc catggggaca gactgggccc gggaagcggc
      601 aaagtgaaat gagacaacgg gcttttcggg tgggccaccc cggataaccc acatgtaggc
      661 ggaattggcc ttctgcttct tgtccttctt gccccccaga aaggaaacgg tggtttcatc
      721 aatgcccagc atttccccat ccagggcgga aagctccaga agctccgcca gcgggagaca
      781 gcgctcgtac agggccatgg cccagttgca catgctggcc cggctcacct caacgccata
      841 acggctcagc atgttttcct gacggtgaaa gggcagggca tcggtaaatt tgcccgtcat
      901 gatgtgggcc agcataccgg gactgaccat gcacttggga agaatctggg cgggaggggg
      961 ggcaattttg acggcctttt catcgctctg ctcgccatgg cagtgggagc aggcgtactt
     1021 gaaccgcacg tgctgaataa cctgaacggt ggcgggaacg atgtccagct gctcactgct
     1081 ctcctcgccg atgcagtcca tccgggcgcc gcagccacag accttctcct cttcgggaat
     1141 gtcgtgttcc acgcgatgac ggggcagatc ctccggaaaa ggtttgcgac cggtcttggc
     1201 ccgggtatgg ggctttacct ccacttcctc ttgcggggtc tcctgcgtcg tgtcctgctg
     1261 ctcgaagagc tcgggaaaca acagacgccc catctcctcc ggagacgtca gcttctcgga
     1321 cttgcgtccg tacagctggt ggcggagcag acggatctgt tcgcgcagga tgttgttctc
     1381 gtgctcgacc cggtcctttt cacgctccgt ccccaccagc ttgtggtgga cttccgccac
     1441 aaggctctgg agctctgaaa cgctgtctgg gagcgggttt tccatgactc cattatacca
     1501 cgcggct
//
LOCUS       DAOTHD010000614         1505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_100017_length_1505_cov_3.497241, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000614 DAOTHD010000000
VERSION     DAOTHD010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100017_length_1505_cov_3.497241"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="P5219_09755"
     CDS             complement(<1..429)
                     /locus_tag="P5219_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HRX27088.1"
                     /translation="MMKMTKGRIVLISGILAAASFVLKEMIGYEKITIFLMLATTVIA
                     GTPVFRKAIGALRYRIVGIDALVTIAVTGAVIIGEYWEAAAVTFLFMLGDYLEARTIE
                     KTRSSIKALLDMAPDKARVRRNGAETEIDPEDVVKGDLVVV"
     gene            complement(449..664)
                     /locus_tag="P5219_09760"
     CDS             complement(449..664)
                     /locus_tag="P5219_09760"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HRX27089.1"
                     /translation="MTNRTFQLETVSCPSCIAKIEGMLKKTGGISSSEVLFNSSRVKV
                     SFDDSAVTAEEIRAKIESLGYRVLSEK"
     gene            complement(934..>1505)
                     /locus_tag="P5219_09765"
     CDS             complement(934..>1505)
                     /locus_tag="P5219_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRX27090.1"
                     /translation="IEAARAGEAGRGFAVVAEEVRKLAEESNEAAKKIAELAGMITKD
                     LDQVVSSSEKSAKNSHASAGLAEGTRETIAKMMEALSRISSSTQDLAAVSEEQAASSE
                     EIAGAVQTIASRVSASAASSDMVRGQMKEVAESAERVAQGSEELASLSAELRKLVGYF
                     RFEHEGEQRGLVPAKVNTAQKRNNPSKGR"
BASE COUNT          278 a          479 c          372 g          376 t
ORIGIN      
        1 caccaccacg aggtcgcctt tcaccacatc ttccggatcg atctccgttt cggcgccgtt
       61 tcttcgcact cttgccttat ccggcgccat atcgagcaga gccttgatgg aagacctcgt
      121 cttttcaatg gtcctggcct caaggtagtc gcccagcatg aacagaaagg ttaccgcggc
      181 cgcctcccag tactcgccga tgataacggc tccggtaacg gcaatggtta ccagggcgtc
      241 gatcccgacg atgcgatacc tcagcgcccc gatggccttc ctgaaaacag gggtgcctgc
      301 aattacggtt gtggcaagca tcagaaatat cgtgatcttt tcgtatccga tcatctcttt
      361 cagcacgaag gatgcagcgg ccagaatccc cgagatgaga acaatccgcc ctttcgtcat
      421 tttcatcatt gcagacaccc ccttcttcct acttttcgct caggacccgg tagcccaggc
      481 tttcaatttt cgccctgatc tcttctgcgg tgacagcgct gtcgtcaaag ctcaccttta
      541 ctcttgatga attgaacagc acctctgaac tgctgatgcc tccggttttt ttcagcatac
      601 cctcgatttt cgctatgcag ctcggacagg aaacggtttc cagctgaaac gttctgtttg
      661 tcatgaatat gacctccctt tatgtgtggt aggctgagga tacaggaggc ccggatttcc
      721 ttccttgacg caggtcaaaa ttttaaaaaa cctcaaattg tggtcattcc gaagaggcca
      781 gtttaaagga tagcgggatc tgatttttcc tctcattccc gaggagcgaa aaagagagat
      841 ccggaacagg gtaccgccct gatccggatc tctctttttg cttgccggaa cgtcccgagt
      901 agtatggacg gactgccggc cgattcttct tacctatctt cccttggagg gattgtttct
      961 tttctgggct gtatttacct ttgccggtac aagcccccgt tgttcccctt cgtgctcgaa
     1021 ccggaagtac cccaccagtt tccggagttc cgccgacagg ctggcgagtt cttctgagcc
     1081 ctgggccacg cgctccgccg attcggcgac ttccttcatc tgcccccgga ccatgtccga
     1141 ggacgccgcc gaggcactga cccgggacgc gatggtctgc accgccccgg cgatctcctc
     1201 gctggacgcc gcctgctcct cggagacagc cgcaaggtcc tgggtcgagg acgaaattct
     1261 tgacagggct tccatcatct tcgcaatggt ctcccgggtt ccttccgcga gccctgcaga
     1321 ggcgtgggag ttcttcgcgc tcttttccga cgaggagacc acctggtcca gatcctttgt
     1381 aatcatcccc gcaagctccg cgatcttctt cgccgcctcg ttgctttcct cggcgagctt
     1441 ccgcacttcc tccgccacca cggcgaagcc ccgtccggcc tctccggccc gggcggcctc
     1501 aatgg
//
LOCUS       DAOTHD010000615         1505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_100044_length_1505_cov_2.791034, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000615 DAOTHD010000000
VERSION     DAOTHD010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100044_length_1505_cov_2.791034"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="P5219_09770"
     CDS             <1..671
                     /locus_tag="P5219_09770"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF036996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HRX27091.1"
                     /translation="VIFTAATLPSDSRGSDASINLYQSVLASFSRECWGMGVLVCLQD
                     QIFAASEITEAASQRKNSFSAPDRGPVAQFIGERVDILRQKRRAKVLEEKGSPARDVE
                     LLFASLGANDKMVEFLASDEKRELDGLVIAGFGNGNVPPSWIPHIKKLVKDNIPVVIT
                     SRCPKGHTRGMPYAFEGNMARLLEIGVLDGGGLRPIQARLKLAVALGSGLSRSELQSY
                     LLEE"
     gene            868..1293
                     /locus_tag="P5219_09775"
     CDS             868..1293
                     /locus_tag="P5219_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HRX27092.1"
                     /translation="MKYAATDEMIALRLSEGDDLGDSIAHVCRTCDVDSAVIVSAAGM
                     VSSVTFGWYNGRDYNTETVKDIMELASLSGNVSYRGGTLYPHLHGVFNKPDHGAVSGH
                     ILQAIVFNNAEIFLKPLSTVMLGRAFDGAFEALAPEKRL"
     gene            complement(1324..>1505)
                     /locus_tag="P5219_09780"
     CDS             complement(1324..>1505)
                     /locus_tag="P5219_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRX27093.1"
                     /translation="QVGGVPPVGYPEEIPALLDEDLFLYPVVWAAAGSDHAFFPVSPE
                     DLQNMTGGRKCPVRK"
BASE COUNT          353 a          417 c          421 g          314 t
ORIGIN      
        1 cggtcatctt cacggccgcg actcttcctt cagacagcag gggatctgac gcatccatca
       61 acctgtacca gtccgtcctg gcttcctttt ccagggaatg ctggggaatg ggagtccttg
      121 tatgccttca ggaccagatt tttgcagcat ccgaaatcac ggaggctgcg agccagcgga
      181 agaattcctt ttccgctccc gacagaggcc ccgtcgccca gtttatcgga gaaagggttg
      241 acatcctgcg acagaagcgc cgcgccaagg tcctcgaaga aaaaggcagc cccgcgaggg
      301 acgtagaact gcttttcgca tccctcggcg caaatgacaa aatggtggaa tttctcgcct
      361 ccgacgagaa aagggaactt gacgggctgg tcattgccgg gttcggaaac ggaaacgtcc
      421 ctccttcctg gattccccac ataaaaaagc tcgttaaaga caacataccg gtagtgataa
      481 cctcccggtg ccccaagggc cataccaggg gaatgccgta tgcgtttgaa ggaaacatgg
      541 cccggctgct ggaaatcggg gtacttgacg gagggggact cagaccgatc caggcacggc
      601 tgaaacttgc cgttgccctt ggctccgggc tttcacggtc tgaactgcag tcttatctcc
      661 tggaggagta aacctgtccg ctccctgcga ctctgcagga gaggatgttc ccggatttca
      721 ccgggaacat cctctctttt tttatcgtgt cccaaggaaa gagttggagg aaaacccgcc
      781 aagtggtaga atattcgtgc aatatcccgt gcccaaagga tggaatgcag tttaccgcag
      841 gcaaagatcc gaaaggggga atagacagtg aaatatgccg ccactgacga aatgatagcg
      901 ttgcgccttt ctgaagggga tgatctcgga gactccatag cccacgtgtg ccggacctgc
      961 gatgtggatt ccgcggtaat cgtcagcgca gccggaatgg tctcctccgt gaccttcggg
     1021 tggtacaacg gcagggacta caacacggag accgtgaagg acatcatgga gctggcatcc
     1081 ctcagcggaa acgtcagcta caggggaggg accctctacc cccatctcca cggagtcttc
     1141 aacaaacccg accatggagc ggtttctggg catatcctgc aggccattgt tttcaataac
     1201 gcggagattt ttctcaagcc tttatcgaca gtcatgctcg gaagagcctt tgacggagcc
     1261 ttcgaagccc ttgccccgga aaaaagactg taagacggaa ggccttaaca ggccttccgt
     1321 cttttatttc cggacggggc attttctgcc tccggtcatg ttctgaaggt cctcgggcga
     1381 aacggggaag aatgcgtgat cgctccccgc tgcagcccaa actacgggat agaggaaaag
     1441 atcctcgtcc aggagggcgg gaatctcctc cgggtagcct accggaggaa ctcctccgac
     1501 ttgga
//
LOCUS       DAOTHD010000616         1503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_100216_length_1503_cov_3.447514, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000616 DAOTHD010000000
VERSION     DAOTHD010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100216_length_1503_cov_3.447514"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1267)
                     /locus_tag="P5219_09785"
     CDS             complement(<1..1267)
                     /locus_tag="P5219_09785"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HRX27094.1"
                     /translation="MFRKIRSFALPLLLLFLLAAAGEACTTILVTKGASADGSVMVSH
                     SDDNDLMDQRIIYVPAQKHAPGAVREIFCTAAAMGEFPQYNTFSYPRMVTSKRGPGYD
                     TKGPATIPLGTIPQVPETFAYFDGSYGIMNERQLMFGECTNGAKAAPGPEPGRRIMYS
                     AELSRIALERCTTAREAIRLIGALIEEYGLYATGETLPVADTEEGWILEMAPSPEGTG
                     GLWVAKKVPDGEIFVGANQFRIREVDPDDPDMMFSENLHAVAQKHGWWKPEDGKLDWL
                     KTVSLGEYNHPYYSLRRVWRVLSLAAPSKNFSPWVKDGFTKDYPFSVKPDKKLGVRDV
                     MRLHRDHYEGTEFDLTKGTAAGPFGYPDRYYGPYDGAGDVGDPKRKLGGAWERPLSVT
                     YCGYVFVNQARGWLPDAIGGRMWLGLDKPS"
BASE COUNT          262 a          450 c          493 g          298 t
ORIGIN      
        1 cggagggctt gtcgagaccg agccacatgc gcccgccgat ggcgtccgga agccagcccc
       61 tggcctggtt gacgaagacg tacccgcagt aggtcaccga caggggccgc tcccatgcgc
      121 ctcccagctt ccgcttggga tcgcccacgt caccggcccc gtcgtaggga ccgtagtacc
      181 ggtcggggta cccgaaggga ccggcagccg ttccctttgt caggtcgaac tccgtcccct
      241 cgtagtggtc cctgtggagc cgcatcacgt ccctcacgcc cagtttttta tccggtttca
      301 cggagaatgg gtagtccttc gtgaagccgt ccttcaccca gggggagaag ttcttcgacg
      361 gcgcggccag ggagagaacc ctccagaccc tccgaaggga gtagtaagga tggttgtatt
      421 cccccaggct caccgttttc agccagtcga gctttccgtc ctccggcttc caccagccgt
      481 gtttttgggc cacggcgtgg aggttttcgg agaacatcat gtccgggtcg tccggatcca
      541 cctcccggat cctgaactgg ttggcaccca cgaaaatttc accgtcggga actttcttcg
      601 ccacccacag ccctcccgtc ccttcggggg agggggccat ctccaggatc catccctctt
      661 ccgtgtccgc cacggggagg gtctcgccgg tggcgtagag gccgtattct tctatcaggg
      721 cgccgatgag ccggatggcc tccctggcgg tggtgcaccg ctcgagggcg atccgggaga
      781 gctcggccga gtacatgatc cgcctgcccg gctcgggccc cggggcggct ttcgcgccgt
      841 tggtgcactc gccgaacatg agctgccgct cgttcatgat gccgtagctt ccgtcgaaat
      901 aggcgaaggt ctccgggacc tgggggatag ttcccagggg gatggtggcc ggtccctttg
      961 tgtcgtaccc ggggccccgt tttgatgtga ccatccgggg ataggagaag gtgttgtact
     1021 gggggaattc ccccatggcg gctgcggtgc agaaaatctc cctcacggcg ccgggagcgt
     1081 gtttttgtgc ggggacgtag atgatccgct ggtccatgag gtcattgtcg tccgagtggg
     1141 agaccataac ggagccgtcg gcggaggcgc ctttcgtcac caggatggtg gtgcacgctt
     1201 ctccggcggc ggccaggaga aaaagaagga gcagcggcag ggcgaaggac cggatttttc
     1261 ggaacatggg aaccctcctt gaagtgtggg gataactgcc tgtttcggtc tgattataga
     1321 ccccaggacg gaggccgcaa ggtttttccg aaaagagccg accctttcgc gcaggataca
     1381 ttttccgggg aatccgctat actggcagga aaaggaccct gctcccggga acgggttctc
     1441 cggcagccgg gaaagcgggg cagaaaaggg agagtgtcat ggagaaaaca gggagcattt
     1501 ccg
//
LOCUS       DAOTHD010000617         1501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_100452_length_1501_cov_2.401798, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000617 DAOTHD010000000
VERSION     DAOTHD010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100452_length_1501_cov_2.401798"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="P5219_09790"
     CDS             <1..531
                     /locus_tag="P5219_09790"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRX27095.1"
                     /translation="AKAMEDGGALVEEGQPKGMEDSYEIMMGLLSADGGASIRRLLRE
                     SGTERHSIPWLGLGSPMESAAFDALIMRWYGFRSSMLSFLENRDVILSPVNALPAQPH
                     GAVRDDLRAFSYTMAYNLTGWPVTGVRAGTSSSGLPIGVQIAARPWREDVSLAAAAWV
                     ERELGAFPPPDLSRER"
     gene            complement(606..1247)
                     /locus_tag="P5219_09795"
     CDS             complement(606..1247)
                     /locus_tag="P5219_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27096.1"
                     /translation="MTDLELLAGLETEIPGWVLQNRLRENGYVNVWQKIEGLVSDGYI
                     ERLKRGWYCLGSLVRRREPSLRYLTCNICGPAAVSGNLVLFEAGIIPDVVASVTAVSS
                     KRRSSLMTPYGNIDWERLPSNLVFPGTCRVEETPGYIRATKEKALLDQLYITRYTPQN
                     YSLWKAFVFDDLRMDEDALAGLDFALMQELARLFTSAKITKYMHWFLKYFGTS"
BASE COUNT          311 a          426 c          423 g          341 t
ORIGIN      
        1 gcgaaagcga tggaggacgg cggcgccctg gtcgaggagg ggcagccgaa gggtatggaa
       61 gactcgtacg agatcatgat ggggctgctg tccgccgacg gaggggcctc catacggagg
      121 ctgctgcggg agtcgggtac ggaacggcac tccattccgt ggctcggcct tgggtctccc
      181 atggaaagtg ccgctttcga cgcactgatc atgagatggt acggcttccg cagttccatg
      241 ctttcgtttc tggagaaccg ggacgtgatt ctttcgccgg taaacgcgct gccggcacag
      301 ccccacgggg cggttcggga cgatcttcgg gcgttcagct acaccatggc ctacaatctc
      361 accggctggc ccgtcacggg ggttcgtgcc gggacgtcat cgtcgggcct cccaatcggg
      421 gtgcagatcg cggcccgtcc ctggagggaa gacgtgtctc tggccgccgc cgcatgggtg
      481 gaacgggagc tcggggcatt cccgccgccc gatctttccc gggagaggtg atttccggct
      541 aaactcgcct ctgtgcgccg ccatgagccg gaaagagctg gtcgtcattc gggcagtctc
      601 ccgattcagg acgtgccgaa gtacttcaga aaccagtgca tgtattttgt tatcttcgca
      661 gacgtgaata gccgtgccag ttcctgcatc agggcaaaat ccaggccggc cagggcgtct
      721 tcgtccatcc tcagatcgtc aaagacaaaa gccttccaga gagagtagtt ctgcggggta
      781 tacctcgtta tgtatagctg atcaagcagg gccttttctt tcgttgccct gatatatccg
      841 ggcgtttctt ccaccctgca ggtgcccgga aagaccagat ttgatggaag cctctcccag
      901 tcgatgtttc cgtaaggagt catcaggctg gacctccgct tcgaactgac ggcagtgacg
      961 gatgcaacga catcaggtat aatacctgcc tcaaagagca cgaggtttcc ggatactgcc
     1021 gccgggccgc agatattgca ggtaagatac cgcagggagg gttctcttct gcgcacgagg
     1081 gatcccaggc aataccatcc gcgcttcagc cgttcgatgt acccgtccga taccaggccc
     1141 tctatcttct gccatacatt gacgtatccg ttctcgcgaa gcctgttctg caagacccag
     1201 ccggggatct ccgtctccag gcccgcaagc agttccagat cagtcatgtc gtcatcatcc
     1261 cgtcttcatt ttctatttta tgtatgttaa catctaaaaa aaagatttta cgctacataa
     1321 aaagaaaatt ccaggccatc acaccctgag gtttcaggag gatccttcct gtggaatttt
     1381 gtgtgaaagg agaaatcgtg cagctgcatc aacgagtttt tccttctgct gaaggaacat
     1441 ctctgtactc catgcagcgg aactcctctt ttcgagtgca tcagtcataa aaccgccgac
     1501 a
//
LOCUS       DAOTHD010000618         1497 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_100780_length_1497_cov_2.843273, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000618 DAOTHD010000000
VERSION     DAOTHD010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100780_length_1497_cov_2.843273"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="P5219_09800"
     CDS             <1..1110
                     /locus_tag="P5219_09800"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRX27097.1"
                     /translation="KGSLEQIGEIVSSYAPTSVAFLVDGNVRKNGGADRILELLAVQG
                     AELHVFSDLPVEPETGQVKDLFGKLSGLGVDLVVAMGGGSVLDSAKMVAVLLRNPAYL
                     DNLLDAQLIRERGVPLIAIPTSAGTGSEATPNSIVVVPEQKLKVGVVHPFFIPDCVVL
                     DPSVTLTLPPNVTAATGLDAFCHAIECFISKKRNPFSDLYALEAIRLISRSLMKAYKD
                     GSDLQAREDLLLAAFYGGMCIASSSTVAVHALSYPLGGRYRIPHGISNAILLPWVMEF
                     NRDTVTDRFTEVASAMGIPPGADDGETSKKTVEGIFSLVRSLGIPSDLRELGVRKEDL
                     DEIVAAAMKVTRLLDNNPKPVTPEDARNIYLKLLP"
     gene            1158..>1497
                     /locus_tag="P5219_09805"
     CDS             1158..>1497
                     /locus_tag="P5219_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRX27098.1"
                     /translation="MIDIHGVIPALVTPMNPDESVNEKELRNQIRRQIAAGVHGLFVF
                     GTNGEFYALSEAEKLRVLEITVEENNGRLPVYAGTGCPGTADTVRLSRECEKIGADAL
                     SVITPYFAALS"
BASE COUNT          304 a          483 c          442 g          268 t
ORIGIN      
        1 aaaggaagcc tggaacagat aggggagatt gtttcttcct acgctccaac gtccgtcgcc
       61 ttcctcgtgg acggaaacgt gcggaaaaac ggcggagcgg acaggatcct ggagctgctg
      121 gccgttcagg gtgcggaact ccacgttttc agcgatcttc ccgtggagcc cgaaacaggc
      181 caggtgaagg accttttcgg aaaactgagc ggcctcgggg tggacctggt ggtggccatg
      241 gggggcggaa gcgtcctcga cagcgccaag atggtcgccg tgctcctccg gaatccggcc
      301 tacctggaca acctgctgga cgcacagctc atcagggaac ggggcgttcc cctgatcgcc
      361 atacccactt ctgccggaac gggttccgag gcgacaccga actccatcgt ggtggtgccg
      421 gaacagaagc tgaaagtggg ggtggtccat cccttcttta ttccggactg cgtcgtcctc
      481 gacccctccg tcacgctgac cctcccgccg aacgtcaccg ccgccaccgg cctggatgcc
      541 ttctgccacg ccatcgaatg cttcatctcg aaaaaaagga atcctttttc tgacctctat
      601 gccctggagg ccattcggct catcagccgc agcctgatga aggcctataa agacggatcg
      661 gatctccagg cacgggaaga cctgcttctc gccgccttct acgggggcat gtgcatcgcc
      721 tcctccagca ctgtggccgt ccacgccctc tcctatcccc tgggcgggcg gtaccgcata
      781 ccccacggca tctccaacgc catcctcctt ccctgggtca tggagttcaa ccgggatacg
      841 gtgacggaca ggttcacgga ggttgcctcc gccatgggca tccctccggg ggccgatgac
      901 ggggagacgt ccaaaaagac cgtggaaggc atcttctccc tggtgcgctc cctgggcatc
      961 ccgtcggacc tcagggaact gggggtccgg aaagaagacc tggacgagat cgtcgcagcg
     1021 gcgatgaagg taacccggct gctcgacaac aacccgaagc ccgtcacgcc ggaggacgcc
     1081 cggaacatct acctgaaact gctgccgtag gggacctttc ggtcctgtac aagaccacat
     1141 tgaatggaga gaagcccatg attgatattc acggagtcat accggcactg gtcacaccca
     1201 tgaacccgga cgaaagcgtc aatgaaaaag agctccggaa ccagatccgc cgccagatcg
     1261 cggcgggagt tcacggtctc ttcgtcttcg ggacgaacgg cgagttttac gccctgagcg
     1321 aggccgaaaa gctccgggtg cttgaaatca ccgtggaaga gaacaacggg cgccttcccg
     1381 tctacgccgg aacgggctgc cccggaacgg cggatacggt gcgcctctcc agggagtgcg
     1441 aaaagatcgg cgccgacgcc ctttcggtca tcacgcccta cttcgccgcc ctctccc
//
LOCUS       DAOTHD010000619         1496 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_100850_length_1496_cov_3.472588, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000619 DAOTHD010000000
VERSION     DAOTHD010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100850_length_1496_cov_3.472588"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="P5219_09810"
     CDS             complement(<1..834)
                     /locus_tag="P5219_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRX27099.1"
                     /translation="MNEEYRGVWTLSEVREGRIHPVSYELLAWGRDLADTLGVPLSAV
                     LLGSGVTEQAPELIVHGADQVYVVDDPNLAHFKADSFSKTLAELVNRHRPEIFIASAT
                     SSGRTVMPVLYADLETGLTADCTELGIDTKERLLLQTRPAIGGNVMADIKTPLRRPQM
                     CTVRPRSKKPLPPDRSRTGRIIAEKAVPGSLSSVMKLLEFLPEKNVGAPLQDAEVIVA
                     GGKGMKKGANFALLEELAGLLGGSVGASRTAVDLNWAPYSSQVGLSGKSVTPKLYIAC
                     GI"
     gene            complement(847..>1496)
                     /locus_tag="P5219_09815"
     CDS             complement(847..>1496)
                     /locus_tag="P5219_09815"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRX27100.1"
                     /translation="RLGEGTEITVISMGPPQAQKAVRAAIAMGCDRGLLLSDRRFAGS
                     DTLATARTLAAGVQKAGGFDLLLSGERATDGETGQVGPAVGQILGLSVLTFVSAVESA
                     AKGSLVVRRAVEGGHERVEAPLPAMLSVVKEINTPRLCTLGGKIRAKKTEIPVLGADD
                     LPIPPEHLGLPGSPTKVVKVSYPQITRSGKKVAAADSLNGAVEEILSVLRGRGIV"
BASE COUNT          241 a          498 c          465 g          292 t
ORIGIN      
        1 gatgccgcag gcgatgtaga gcttcggcgt caccgatttg cccgacagcc ctacctggga
       61 ggaatagggc gcccagttca ggtcgacggc ggtccgcgat gctcccaccg agcctccgag
      121 gagaccggcc agctcttcca gcagggcgaa attcgccccc ttcttcatcc cctttccgcc
      181 cgccacgatg acctcggcgt cctgcagggg tgcgcccacg ttcttctccg gcaggaactc
      241 caggagcttc atcaccgacg aaagggagcc gggaacggcc ttctcggcaa tgatcctgcc
      301 ggtccgcgac cggtccggag gaaggggttt cttcgacctc ggcctcacgg tgcacatctg
      361 gggccttctg aggggcgttt tgatgtccgc catcacgttt cccccgatgg cgggcctggt
      421 ctggagcagc aggcgttctt ttgtgtcgat gcccagttcg gtgcagtctg ccgtcagccc
      481 cgtctcgagg tccgcgtaga gcactggcat cacagtcctg ccggaggagg tggccgaggc
      541 gatgaaaatt tccggcctgt gacggttcac cagctcggcc agggttttgg aaaaactgtc
      601 cgctttgaaa tgagccaggt tcgggtcgtc caccacgtac acctggtcag ctccgtggac
      661 gatcagttcc ggagcctgtt ccgtaacgcc ggagccgaga agcaccgccg acaggggaac
      721 gccaagggtg tctgcaaggt cccttcccca ggcgagaagt tcgtatgaaa cgggatgtat
      781 cctgccctcc cgcacttcgc tgagggtcca gactccgcgg tattcttcgt tcatggcggt
      841 ttcctcctac acgatcccgc ggccccggag cacgctgagg atttcctcaa cggcgccgtt
      901 caggctgtct gcggcggcta ctttctttcc cgaccgggtg atctggggat aggacacctt
      961 caccactttc gtcggcgagc ccggaaggcc gaggtgctcc ggaggaatgg gaagatcgtc
     1021 tgccccgaga acggggatct cagtcttctt cgcccggatc tttcctccca gggtgcacag
     1081 ccggggggta ttgatctcct tcaccaccga gagcatggcc ggaaggggtg cctcgacccg
     1141 ctcgtgacct ccctccaccg cccgccggac tacaagggag ccctttgcgg ccgattcaac
     1201 ggcgctcacg aaggtgagca ccgacagccc gaggatctgc cccacggcag ggccgacctg
     1261 ccccgtttcg ccgtcggtgg cccgttcgcc cgagagcagg aggtcgaagc cgccggcctt
     1321 ctgtactccc gctgcgaggg tcctggcggt ggccagggta tcggacccgg caaaccgcct
     1381 gtcggacaga agcagtcccc tgtcgcaccc catggcgatg gcggcccgta ccgctttctg
     1441 tgcctggggc ggccccatgg agattactgt tatctccgtt ccttctccga gacggt
//
LOCUS       DAOTHD010000620         1495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_100962_length_1495_cov_2.812500, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000620 DAOTHD010000000
VERSION     DAOTHD010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100962_length_1495_cov_2.812500"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="P5219_09820"
     CDS             complement(<1..363)
                     /locus_tag="P5219_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HRX27101.1"
                     /translation="MDPVGLILIFCARIVDVSCGTVRILFLVRGKRALAAAIGFVEVM
                     VYLTALGHILGGGREMNLLQMVVYCAGFSSGNFIGSLLEERLLNAYVLLELIMDRNPE
                     TQEMIDNIRQDGYGATVLY"
     gene            550..>1495
                     /locus_tag="P5219_09825"
     CDS             550..>1495
                     /locus_tag="P5219_09825"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRX27102.1"
                     /translation="MDKDRTIMLLEERVSFLSLERQAALRAFETALDLNAFTASLTGL
                     DSLQEIFRETASKLRSMERFGPLAFFLAGEEDGALYPAFSDPPGEEKILERELVPLIE
                     DRTVAWTLRRKRPILVSRSDGQGKLFLHSLSSPSKILGLFLAVLGNNAEDTNYEPSFP
                     DFLSIVLTTAAGLADNLLLRARIDELNMNLQEKVHHLEISKEELSIYRDNLEREVEIR
                     TRELETANEELRKEIEERRRMEAEVRYRAYHDALTGLANRDLFSARLQETLDRNEPVA
                     VLFMDLDGFKGVNDTLGHDVGDRLLQKIARRLTLAVREG"
BASE COUNT          369 a          438 c          400 g          288 t
ORIGIN      
        1 gtacagcacc gtggccccat agccgtcctg ccggatgttg tctatcattt cctgcgtctc
       61 cggattcctg tccatgatca gttcaaggag cacgtaggcg ttcaggagac gttcctccag
      121 aagggacccg ataaaattgc ccgacgagaa ccccgcgcag tagaccacca tctggagaag
      181 attcatttcc cttccgcccc ccaggatatg cccaagggcc gtaaggtaca ccatcacttc
      241 cacgaaaccg atggcggcag cgagggctct cttccccctg acaagaaaca ggatccggac
      301 cgtgccgcag ctcacatcca ctatcctggc gcagaaaatg agaatgagcc ctaccggatc
      361 catgaaatcc gtccggaaca aaatgttgtc ttcattgaaa aacctccttg caaaacatcc
      421 atattatcct accatagagc gaaaaaagaa aaagggttcc ggtacggaaa ggattgcgcc
      481 atcgtataat ggaaccgttt ttttcaacct gagacagaca ctcacccccg agaggtgtga
      541 tgatgcacaa tggataaaga ccggacgatt atgcttcttg aagaacgggt gtcctttctt
      601 tcactggaaa ggcaggcggc cctccgcgcc tttgagacgg cccttgacct caacgctttc
      661 acggcgtcgc tcacgggact cgattccctc caggaaatct tccgggagac ggcatcaaaa
      721 ctgcggtcca tggaacggtt cggtccgctg gccttttttc tggcgggaga agaggacgga
      781 gcgctctacc ccgctttctc cgatcctccg ggggaagaga aaatcctgga gcgggagctt
      841 gttcccctta tcgaggaccg gacggtggcc tggactctca gaagaaaaag acccattctt
      901 gtctcccggt ccgatggaca gggaaaactt ttcctccatt ccctgtcgtc cccttcgaaa
      961 atcctcgggc tgtttctcgc cgtactcgga aacaacgccg aggacacaaa ctacgagcca
     1021 tccttcccgg attttctatc catcgtcctc accactgcag cggggctggc ggacaatctt
     1081 cttctcaggg caaggatcga cgaactgaac atgaacctcc aggaaaaagt gcaccatctc
     1141 gaaatatcga aagaggaact ctccatctac cgggataacc tcgaacggga agtcgagatc
     1201 cggaccaggg aactggaaac ggcaaacgag gagctgcgga aggagataga ggaacggcgg
     1261 cgcatggaag ccgaagtgag gtacagggcg taccacgacg ccctgaccgg gctcgcgaac
     1321 agggatctct tctccgcccg cctccaggag accctcgaca gaaatgagcc cgtggccgtg
     1381 cttttcatgg acctggacgg attcaagggc gtcaatgaca ccctcggcca cgacgtgggg
     1441 gaccggctgc tgcagaaaat cgcccggagg ctgacgctgg cggtaaggga aggcg
//
LOCUS       DAOTHD010000621         1495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_100975_length_1495_cov_2.516667, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000621 DAOTHD010000000
VERSION     DAOTHD010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100975_length_1495_cov_2.516667"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="P5219_09830"
     CDS             <1..377
                     /locus_tag="P5219_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27103.1"
                     /translation="NFAGKLVGHLRGLFKSVDREEGERTIVRPPFSFFGALTIDSTAI
                     EDLVRHVVSLSAQVDAVKDIRVRSSEDEISLEITVNVTPGKFNFRQLGKVQQKRVASA
                     VRYFTGMDIGSVDISIAEVVLP"
     gene            374..1003
                     /locus_tag="P5219_09835"
     CDS             374..1003
                     /locus_tag="P5219_09835"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRX27104.1"
                     /translation="MTLFQSSADREEAWAELQKKVECCTRCGLCSSRKKTVFGQGHRN
                     TPLVFIGEGPGAEEDEQGVAFVGRAGRLLTQILASVGIRREQVFISNVVKCRPPDNRV
                     PAPDEMMACSPYLEAQLALLRPRIVVCLGNTPTKWLLRTSEGITALRGRWFPWRGALL
                     LPMFHPSFLLRNESRKKGSPKELTWRDINAVREKLSELQARLPEEEGNP"
     gene            1000..>1495
                     /gene="uppS"
                     /locus_tag="P5219_09840"
     CDS             1000..>1495
                     /gene="uppS"
                     /locus_tag="P5219_09840"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HRX27105.1"
                     /translation="MKAYPVPSPFHVALIMDGNGRWAKKRFLPRLLGHRAGVKALERT
                     VRAAGNMGVTHLSVYAFSTENWKRPEMEIKGLMSLFRFSLRRKVDEIKRENVRLRFAG
                     KRDNLPPDVADLMDWAEKETGMNTGLTLVACLNYGGRQEILDGVNELIAEGRSRPLTE
                     EDLDR"
BASE COUNT          336 a          369 c          487 g          303 t
ORIGIN      
        1 aaaatttcgc aggcaagctg gtggggcacc tgagaggtct tttcaaaagc gtggacaggg
       61 aagaggggga gcggaccatc gtgaggcctc ccttcagctt tttcggggcg cttaccatcg
      121 actcgaccgc tatagaggat ctggtgcgcc atgtggtatc gctgagtgcc caggttgatg
      181 ccgtgaaaga cattcgcgtg cgctcgtcgg aggatgaaat ttctttggaa attactgtga
      241 acgtgacacc gggaaagttc aatttccggc agctgggaaa agtgcagcaa aagcgggttg
      301 cttccgctgt ccggtatttc acgggtatgg atatcggaag cgttgatatc agcatagcgg
      361 aggtggtgtt gccgtgaccc tttttcagag cagcgcggac agggaggaag cctgggcgga
      421 gctgcagaaa aaagtggaat gctgcaccag gtgcggcctg tgctcctcaa gaaaaaaaac
      481 cgttttcgga caggggcatc ggaacactcc cctcgtattc atcggtgaag ggccgggggc
      541 ggaggaagat gaacaggggg tcgctttcgt cggaagggcg ggacgtctgc ttacccagat
      601 tcttgcctcg gtgggcatac ggagagagca ggtctttatt tcgaacgtgg tgaaatgcag
      661 accgccggac aaccgggtgc ctgcccctga tgaaatgatg gcctgcagtc catacctgga
      721 agcccagctg gctcttcttc gtccgcgaat cgttgtctgt ctcgggaata ctccgacgaa
      781 gtggctcctg aggacttcag aagggatcac ggctcttcgg gggagatggt tcccctggag
      841 gggagccctt ctgcttccca tgttccatcc gagttttctg ctgagaaacg aatcacggaa
      901 aaagggaagt ccgaaggaac tgacgtggag ggacatcaat gccgtccggg agaagctctc
      961 tgaactccag gcccggctgc ctgaagagga ggggaatccg tgaaggctta ccctgtccct
     1021 tccccttttc atgtggccct catcatggac ggcaacgggc ggtgggcgaa gaaacggttt
     1081 cttccccgcc ttctgggcca cagggccggt gtaaaggccc tcgaaagaac cgtgcgggcc
     1141 gcaggaaaca tgggagtgac ccacctttcg gtgtatgcct tttccacgga aaactggaag
     1201 cggccggaaa tggagatcaa gggactcatg agcctcttcc gcttttcact ccgccggaag
     1261 gttgatgaaa tcaagcggga gaacgtccgg ctcaggtttg caggaaagcg ggacaacctg
     1321 cctcccgatg tggcggatct catggactgg gcagagaagg agaccggcat gaatacaggg
     1381 ctcactctcg ttgcctgcct gaattacgga gggcggcagg aaatccttga cggagtgaat
     1441 gagctgattg cggaaggcag gagcaggccg ctcacggagg aagacctgga cagga
//
LOCUS       DAOTHD010000622         1493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_101131_length_1493_cov_3.755216, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000622 DAOTHD010000000
VERSION     DAOTHD010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101131_length_1493_cov_3.755216"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..>1493)
                     /locus_tag="P5219_09845"
     CDS             complement(24..>1493)
                     /locus_tag="P5219_09845"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HRX27106.1"
                     /translation="RPIHEEYEITAASGVRKWVLEIGQGVYDHDGNVEALEGIIIDIS
                     SRREYERQLQFMGEHDILTGLYNRAFLEKILARDAGEEDRTVVMVDLNRLNALSLSYG
                     YAFSEQIVREAATRLLALATDERKLFQISPERMAFYLKNSTDAGEAARLCDSIISALS
                     GIQLIHAAGCGIGILRLDETCRDGDAILQNASIAAEYAARKGAFQVCPFEKELEERAK
                     REKDIKEELLETLGNEGSRDIYAEFQPIVDLWTGRIKGFEALARMRSPRLGPVSPLEF
                     IPLSEELQLVVPLGRRITGLVCDFLEEIRRAGHDDVYVSVNVSAIELVRDRFLDDFFG
                     LLEEKETAPSRICLEVTESLLADNFDLINRKLGELQKRGVSVAMDDFGTGYSSLFRAR
                     ELNVNYLKIDRHFIDKLVHLPPEAAITGDIISMGHRLGRLVVAEGVELEEQKFYLQRH
                     RCDLMQGYLFSRPLPPEAALELLGKNTPVEPQTERGERP"
BASE COUNT          266 a          454 c          442 g          331 t
ORIGIN      
        1 tgcccttgca ggtccggata gtctcatggc cgctcgcccc tttcagtctg cggttccaca
       61 ggggtgtttt tccccagcag ttccagcgcc gcctccggcg ggagtggcct gctgaagaga
      121 tacccctgca tcaggtcaca ccggtgccgc tgcaggtaga acttctgctc ttccagctcc
      181 acgccttcgg cgaccacgag gcggccgagc cggtgtccca tggagatgat gtcgccggtt
      241 atggctgcct ccgggggaag gtggacgagc ttgtcgatga agtgcctgtc gatcttgaga
      301 tagttcacat tcagctccct tgcacggaac agggacgaat agccggtgcc gaaatcgtcc
      361 atggccactg agacgcccct tttctgcagc tcgcccagct tgcggttgat caggtcgaag
      421 ttgtccgcca ggagtgattc ggtgacttcc aggcagatcc tggagggagc ggtttccttc
      481 tcttcgagaa gaccgaagaa atcgtcgagg aatctgtccc tcaccagctc gatggccgac
      541 acgttcacgg agacatagac atcgtcatgg cccgccctgc gtatttcttc gagaaaatcg
      601 cagaccagtc ccgtgatcct ccgtccgagg ggaacgacga gctggagttc ctccgacagg
      661 ggaatgaatt ccaggggaga cacgggaccg agcctggggc tcctcatgcg ggccagggct
      721 tcaaatccct tgatcctgcc cgtccacagg tcgacgatgg gctggaattc agcgtaaatg
      781 tcccggctcc cctcgtttcc aagggtttcg agcagctctt ccttgatgtc cttttcccgc
      841 ttcgcccttt cctccagctc cttttcaaag ggacagacct ggaaggctcc cttccgggcg
      901 gcatattccg cggcgatgga cgcgttctgg aggatggcgt caccatcccg gcatgtttca
      961 tccagccgga ggatgccgat gccgcagcct gcggcgtgga tgagctggat cccggagaga
     1021 gcggagatta tggagtcgca gaggcgggcc gcttcgccgg cgtccgtgga gttcttcagg
     1081 tagaaggcca tccgttcagg tgaaatctgg aagagcttcc tttcatcggt ggccagggca
     1141 aggagccggg ttgccgcttc cctgacgatc tgttcgctga aggcgtagcc gtaggagaga
     1201 ctcagggcgt tgaggcggtt caggtccacc atcacgacgg ttctgtcttc ctcccccgcg
     1261 tccctggcca ggattttttc taaaaaggcc cggttgtaca ggccggtcag aatatcgtgt
     1321 tcgcccataa actgcagctg ccgttcatat tccctgcggc tcgagatgtc gatgatgatc
     1381 ccttcaagag cctccacgtt gccgtcgtgg tcgtagaccc cctgcccaat ctccagcacc
     1441 catttccgca cccccgacgc ggcagtaatc tcgtattctt cgtggatggg gcg
//
LOCUS       DAOTHD010000623         1493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_101140_length_1493_cov_3.262865, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000623 DAOTHD010000000
VERSION     DAOTHD010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101140_length_1493_cov_3.262865"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1493
                     /locus_tag="P5219_09850"
     CDS             <1..>1493
                     /locus_tag="P5219_09850"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRX27107.1"
                     /translation="IESLGRHPEKENLFVASVRDGIGTAVVVTAAPNLSEGDRVPYGR
                     PGSVLADGTVLGTREFGSVSSEGMLLSAEELGVPEAADEFGILRLPGDSPVGGDVVRL
                     FGLDDAILDLSITPNRGDLLSLLGVAREVYALFPGAEWKKNPADVAPPERTDDWPVPF
                     GGISLRDDGCSKYCLGLATGLKPVPSPLKTRIALTFLGMRPISGMVDATNIAMLTLGQ
                     PTHAFDAGRLAAPEITVRSAKPGEEITTLDGKRHTLEDSDLLITSAGEAIGLAGVMGG
                     ENSDILPETQTVFIESASFDAIRVSRTSRRLGINSEAAFRYARTVDSNLSELALNFIA
                     SLLKEWGAAETGYVFKTAVSVEPARREVVLTEKNLRKILLTGDMDGASAILERLGLRQ
                     TASEEGKRVFSVPSWRPDIIIEEDLIEEVGRIRGYNETLVPRLPQALFGRGDIGEITR
                     VKGGIRTILLSRGYVELVNYSFLAPSFVGLLRLPPEDRRARPLELAN"
BASE COUNT          291 a          455 c          458 g          289 t
ORIGIN      
        1 cattgaaagc ctcggccgcc atcccgaaaa ggaaaacctg ttcgtcgcaa gtgtgcggga
       61 cggcatcgga accgccgttg tggtcaccgc ggccccgaac ctttcggagg gcgacagggt
      121 tccctacggg cgtcccggct ccgtcctggc tgacggaact gttctgggga ccagggaatt
      181 cggcagcgtc agcagtgagg gaatgctgct ttccgccgag gaactgggcg tacccgaggc
      241 tgcggacgag ttcggcattc tccgccttcc cggagactct cccgtgggcg gcgacgtggt
      301 caggctgttc ggcctggatg atgccatcct tgatctttcc atcaccccga accggggcga
      361 tctcctgagc ctcctcgggg tggcgaggga agtgtatgcc ctgttccccg gggctgaatg
      421 gaagaagaac cctgcggatg tcgctcctcc cgagaggact gatgattggc ccgtgccctt
      481 cggcggcatc tcccttcggg atgacggctg cagcaaatat tgtcttggcc tggcgacggg
      541 gctgaaaccc gttccgtccc ccctgaagac ccggatcgca ctgaccttcc tcggcatgcg
      601 gcccatttcc ggcatggtgg acgctacaaa catcgccatg ctgaccctcg gccagccgac
      661 ccacgccttt gatgccggaa ggcttgccgc tccggaaatc acggtccgtt cggcaaaacc
      721 gggagaagag ataaccaccc ttgacgggaa acggcatacc ctggaagatt ccgacctcct
      781 cataaccagc gccggagagg ccataggcct tgccggtgtc atgggcggcg aaaacagcga
      841 tatccttccg gaaacacaaa cggtgttcat cgaatctgca tctttcgatg cgatccgtgt
      901 cagcaggacg tcccgcaggc tgggcatcaa ctcggaggcg gccttccgct atgccaggac
      961 ggtggactcg aacctgtcgg aactggccct gaactttatc gcttcccttc tgaaagagtg
     1021 gggggcagcg gaaacgggat atgtcttcaa gacggccgtg tccgtggagc ctgcccggag
     1081 ggaagtcgtc ctgacggaaa agaaccttcg gaaaatcctc cttaccggcg acatggacgg
     1141 cgcctcggcg atccttgaaa gactcggact gcggcagact gcctctgaag aagggaaacg
     1201 ggtgttttca gttccctcct ggcgtcccga catcatcatc gaggaagatc ttatcgaaga
     1261 ggtgggcaga atccggggat ataacgaaac gctggttccc cggcttcccc aggccctttt
     1321 cggccggggg gacatcggtg agatcaccag ggtgaagggc ggcatacgga ccatcctcct
     1381 ctcaagggga tatgtggagc ttgtgaacta cagcttcctg gctccatcct tcgtgggtct
     1441 cctcaggctt cctcctgagg acaggagagc ccgccccctg gaactggcga atc
//
LOCUS       DAOTHD010000624         1492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_101236_length_1492_cov_3.033403, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000624 DAOTHD010000000
VERSION     DAOTHD010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101236_length_1492_cov_3.033403"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="P5219_09855"
     CDS             complement(<1..1153)
                     /locus_tag="P5219_09855"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRX27108.1"
                     /translation="MKDVTDVPEAKTGRVARVDLTERRVEIRDTPDFYGNIGGRGFGV
                     FELLRGVTPGTDPLSAENLLVFAAGSLTGTSFPGSSRIALVTKNVLTGGISTSSGGGN
                     LGPKFRQAGFDALVVSGRSSSPCWILLKDGQVEIRDGSDLWGLSVSAAADAVRRVTGD
                     PGAETASIGIAGERLAGVSCVMIGKAHALAWGGSGAVMGFKNLKAVAASGRVYPAAAD
                     GDAFLEESRRYSWVLRASSASAALRTGGTHGMAGVGGWSGKVPTSVRNLQEEFWDGEK
                     SARINEAAFRPLEKKRTACWNCPLSCLHLYETERDGERIEVEGMHANSVRGLGSNLDL
                     DDPAALLEAHRLCNESGLDVDGVAAAVGWAIECFERGILGPGDTDGLELR"
     gene            complement(1254..>1492)
                     /locus_tag="P5219_09860"
     CDS             complement(1254..>1492)
                     /locus_tag="P5219_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27109.1"
                     /translation="EELSPYGNRGVGEHSMISPAPAMDNALFDALGIRIHSYPLGRER
                     VYKAIQAKKKGETDLWEYPYALEQSYKNAIKEWK"
BASE COUNT          266 a          495 c          434 g          297 t
ORIGIN      
        1 agcggagttc caggccgtca gtatcccctg ggccgaggat cccccgctcg aaacactcga
       61 tggcccaacc cactgcggcg gccaccccgt ccacgtcaag gcccgactcg ttgcaaagcc
      121 tgtgggcctc gaggagagct gccggatcgt ccaggtcgag gttggatccc aggccccgga
      181 ccgagttggc atgcataccc tccacctcga tcctctcccc gtcccgttcc gtttcgtaga
      241 ggtggaggca ggagaggggg cagttccagc aggcggtccg tttcttttca aggggacgga
      301 aggccgcctc gttgatgcgg gcggattttt cgccgtccca gaattcctcc tggagattcc
      361 tcacggaagt ggggactttt ccgctccatc cgcccactcc ggccattccg tgggttcccc
      421 cggtccgaag ggcggccgat gcggatgacg cccgaagaac ccaggaatat ctccggctct
      481 cctccaggaa ggcatccccg tctgcggcgg cgggataaac cctgccggag gcggccaccg
      541 ccttaaggtt cttgaatccc atgacggcgc cggaacctcc ccacgccagg gcgtgggctt
      601 tgccgatcat gacgcaggac acgccggcaa gccgttcccc cgcgatgccg atggaggccg
      661 tttcggcccc tggatcgccg gtcactctcc ggaccgcgtc cgccgcggcg gagacggaaa
      721 gaccccaaag atccgaaccg tcccgtattt ccacctgccc gtccttcaga agtatccagc
      781 agggagagga agatcgtccc gacaccacca gggcgtcgaa cccggcctgc cggaacttcg
      841 gcccaaggtt cccgcctccg ctggaggttg atatgcctcc ggtcagaacg tttttcgtca
      901 ccagtgcgat ccgggaactt ccaggaaagg aggtcccggt caggcttccc gccgcgaata
      961 cgagaaggtt ttctgcggac aggggatccg tcccgggggt gacgccccgg agaagctcga
     1021 atacaccgaa tccgcgacca ccgatatttc cgtagaaatc aggagtgtcg cggatttcca
     1081 ccctccgctc cgtcaggtcc acccgggcga ctcttcccgt tttagcttcc ggcacatccg
     1141 tcacatcctt catactgtcg ctctttttct gtcttcaata agttccgggt gcaaccggac
     1201 ggcttttggg cgaccggttg cacaaaaaag attctatcac aggcccagga gaattacttc
     1261 cactccttta tggcgttctt gtacgactgc tccagggcgt agggatattc ccagaggtcc
     1321 gtttccccct ttttcttcgc ctggatggcc ttgtagaccc gctccctgcc gaggggatag
     1381 ctgtggatcc ttattcccag ggcatcaaag agggcgttgt ccatggcggg tgcgggagag
     1441 atcatggaat gctcgcccac ccccctgttg ccgtaggggg aaagctcctc gg
//
LOCUS       DAOTHD010000625         1489 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_101569_length_1489_cov_2.554393, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000625 DAOTHD010000000
VERSION     DAOTHD010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101569_length_1489_cov_2.554393"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="P5219_09865"
     CDS             <1..952
                     /locus_tag="P5219_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HRX27110.1"
                     /translation="VLDNTTNVTASMGVEQIMHALEKGADVIIAGRACDDAVLAAYPI
                     FKGFDRGISLHMGKAAECASLVCWPQKVKESIIGTVYEDHFTIEPIHPDQQATPHSVA
                     AHSMYERTNPFIQALPGGILDMHASKYEAQTDRICKVTGSLFVPSPDGSYKVKLEGAG
                     EAGHRVYHLVGIRDTRAIAHLDQILADTRKKVSEIIGPARDDQYELFFHPYGKNAVMK
                     ENEPVEITQSHEVAIVIEVISKDLELATSVAKCAKFRFFYMSYPGQMNSSGGSVALIT
                     DEPLFPKNKCYQWTVDHLLTLKDPLDPGIFRYTFETVSGV"
     gene            956..>1489
                     /locus_tag="P5219_09870"
     CDS             956..>1489
                     /locus_tag="P5219_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HRX27111.1"
                     /translation="MGAQGTKTLLTELADFYASLGPGDIPGTVLEKARRVLMDFLCET
                     AAGFHEGELASIAIEYARETGGREEATILCDGSKVPAPWAALATGIMAHSIELDDGHR
                     WGTSHPAVAVIPAVLAMGEKMKSSFPDILKGIAVGYDAMLRAARSINPSHLKRGFHST
                     GTCGSIGAAAGCASILGL"
BASE COUNT          311 a          507 c          439 g          232 t
ORIGIN      
        1 ggtgctggac aacaccacga acgtcaccgc gagcatgggt gtggagcaga tcatgcatgc
       61 gctcgaaaag ggagccgacg tcattatcgc cggacgtgcc tgcgacgacg cagtgctcgc
      121 agcatacccc atattcaagg ggttcgacag gggaataagc ctccacatgg gaaaggccgc
      181 cgaatgcgcc tccctcgtct gctggcccca gaaggtgaag gaatccatca tcgggacggt
      241 gtacgaggac cacttcacca tcgagcccat acaccccgac cagcaggcca ccccccacag
      301 cgtggcggcc cactccatgt acgaacggac caaccccttc atccaggccc tccccggcgg
      361 catcctcgac atgcacgcca gcaaatacga ggcccagacg gaccggatct gcaaagtcac
      421 gggcagcctt ttcgtcccct cccccgacgg cagctacaag gtcaagctcg agggggccgg
      481 agaggccggt catagagtat accatctcgt gggcatccgg gacacgcggg ccatcgccca
      541 cctcgaccag atcctcgccg acacgcggaa gaaggtctcc gagatcatcg ggcccgcccg
      601 ggacgaccag tacgaactct tcttccaccc ctacggaaag aatgccgtca tgaaggagaa
      661 cgagcccgtg gagatcactc agtcccacga ggtcgccatc gtcatcgaag tcatctcgaa
      721 ggacctcgag ctggccacct ccgtcgcaaa gtgtgccaag ttccggttct tctacatgag
      781 ctaccccggc cagatgaact cctcgggagg atcggtggcc ctcataacgg atgaacccct
      841 cttcccgaag aacaagtgct atcagtggac ggtggatcac ctgctcaccc tgaaggatcc
      901 actggatccg ggcatcttcc gttatacctt cgagaccgtt tcgggggtgt agaccatggg
      961 agcacagggg acgaagacgc tgctcacgga actggcggac ttctacgcct ccctcggccc
     1021 cggggacata ccggggaccg tcctggagaa ggcccggagg gtgctcatgg acttcctctg
     1081 cgagaccgcc gccgggtttc acgaaggaga gctggcgtcc atcgccatag agtacgccag
     1141 agagaccggc ggcagggagg aagccaccat tctctgcgac ggaagcaaag ttccggcgcc
     1201 ctgggcggcc ctggccacgg ggatcatggc ccactccatc gagctggacg acgggcaccg
     1261 gtggggcacg tcccaccccg ccgtggcggt catccccgcc gtgctggcca tgggggagaa
     1321 gatgaaatcc tccttccccg acatcctgaa ggggatcgcc gtcggatacg acgccatgct
     1381 cagggccgca cggtccatca acccctccca cctgaagcgg ggctttcact caaccggaac
     1441 ctgcggctcc atcggggccg ccgccgggtg cgcctccatc cttggcctg
//
LOCUS       DAOTHD010000626         1488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_101673_length_1488_cov_2.479414, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000626 DAOTHD010000000
VERSION     DAOTHD010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101673_length_1488_cov_2.479414"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="P5219_09875"
     CDS             <1..443
                     /locus_tag="P5219_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27112.1"
                     /translation="PLADDLGGLRKPDGSGGKIREARTGFRRLACAELPFAVSDHPTT
                     RYSLAEIRPQSGRRRQIRRHFKHIFHPIVGDTTYGEGRHNRFFRERFGCARLMLAAVK
                     LSFRHPFTGGRAEIFCPPEENFLSVAGALGWEEAALRNPESGLR"
     gene            complement(455..799)
                     /locus_tag="P5219_09880"
     CDS             complement(455..799)
                     /locus_tag="P5219_09880"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRX27113.1"
                     /translation="MITRNLADFGTMENAHGVDARNIYDTDDAVITVITLKPGQSLKR
                     HITPVDVAFYVLEGKGTVEIGEEKQEVSKDTLIESPKNIMHCWYNTSGADLRFMVVKA
                     PKPNRKTIFPGS"
     gene            complement(796..>1488)
                     /locus_tag="P5219_09885"
     CDS             complement(796..>1488)
                     /locus_tag="P5219_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019677824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX27114.1"
                     /translation="ETAVDALKGVDMAVSPGEVVGLIGPSGSGKTTLLKCLGAVIEPT
                     AGRMTLGNDVIYDGKWRIKDLRALRRDRIGFIFQAPYLIPFLNVTDNVALLPMLAGHS
                     DGESRTRAGELLEALDVAHRASAMPSQLSGGEQQRVAIARALANRPPVILADEPTAPL
                     DSERALSVITILNAMAKQYETAIIVVTHDEKIIPTFRRIYRIRDGRTEETAGEGRSTE
                     KPETGKPEEERA"
BASE COUNT          228 a          462 c          457 g          341 t
ORIGIN      
        1 accccctggc ggatgatctc gggggtctcc ggaagcctga cggcagcgga gggaagatcc
       61 gggaagcccg gaccggcttt cggcgccttg cctgcgcgga attgcccttc gccgtgtcgg
      121 atcatcccac cacccggtat tccctggcgg aaatccggcc gcagagtggg aggcggcggc
      181 agatccggag gcacttcaag catattttcc acccgatcgt gggggatacc acctacgggg
      241 aggggcgtca caaccgtttt ttccgggagc ggttcggctg tgcccggctt atgctggcgg
      301 cggtgaagct gtcgttccgc catcccttca ccggggggag ggctgaaatt ttctgccctc
      361 cggaggagaa tttcctctcc gtcgcggggg ccctcggatg ggaagaggcc gccctccgga
      421 acccggaaag cggcctccgc tgattaacgg cgggtcatga gcccgggaag atggtcttcc
      481 ggttcggttt gggagccttc accaccataa accggagatc ggcgccgctg gtgttgtacc
      541 agcagtgcat aatgtttttc gggctttcga tgagggtgtc tttgctcact tcctgttttt
      601 cctcccctat ttccactgtc ccttttcctt cgaggacgta gaacgccacg tccaccgggg
      661 tgatgtgccg cttcagcgac tggcccggct tcagggtgat caccgtaatg acggcgtcgt
      721 cggtgtcgta gatgttccgg gcgtccactc cgtgagcgtt ttccatggtg ccgaaatctg
      781 caaggtttct cgtaatcatg ctctctcctc ctctggtttt cctgtttcag gtttttccgt
      841 ggaacggcct tcgccggccg tttcttccgt cctcccgtcc cggatacggt aaattctccg
      901 gaaggtggga atgatctttt catcgtgggt gaccacgatg atggccgttt catactgttt
      961 cgccatggcg ttcagtatgg tgatcaccga cagggcccgc tcgctgtcca ggggtgccgt
     1021 gggctcgtcg gccaggatca ccgggggtct gttcgccagg gccctggcta tggctacccg
     1081 ctgctgttcc ccccccgaaa gctgggaggg catggcggat gccctgtggg ccacatccag
     1141 ggcttccagg agttctccgg cccgggtcct tgactccccg tcggaatggc ccgcgagcat
     1201 gggaagcagg gccacgttgt ccgtcacgtt cagaaaaggt atgaggtagg gggcctggaa
     1261 aatgaacccg atcctgtccc tccgcagggc cctgagatcc tttatcctcc actttccgtc
     1321 atagatgaca tcgtttccca gggtcatccg cccggctgtt ggttcgatca ccgcgccgag
     1381 acatttcagg agggtggtct ttccggaccc cgagggtccg atgaggccca ccacctcgcc
     1441 gggagacacc gccatgtcca cccctttcag ggcatccacc gccgtctc
//
LOCUS       DAOTHD010000627         1486 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_101867_length_1486_cov_2.252970, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000627 DAOTHD010000000
VERSION     DAOTHD010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101867_length_1486_cov_2.252970"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..>1486)
                     /locus_tag="P5219_09890"
     CDS             complement(6..>1486)
                     /locus_tag="P5219_09890"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HRX27115.1"
                     /translation="FRAKLPGREFGEGFSAVEFYTGRTGEKGQDARPLPDGSVESAVP
                     FAPGEGLTVVYTWPKGIIAQPSEAAPILERWSRSPYRAVHLAMPVVLLAVMLLFWIRW
                     GKDPAPRPVIPLFSPPAGVEAGFARYVRTMTADDKTFAAMILGMAVKGVLAIEESTLA
                     GEAAKRAGTLPSGAGTALKLLSKLAGTSYRLKLNREKLAGTELTEDERRISDDLFGST
                     RTEIHLSSADRPVIQDAFRRLGLGFRERGRPLLKTNLGKWIAGGVLFELYAVLMLILM
                     ILSGQPRFEPIFALISGPFLLLPLSLPLPSGKGGFIAKFFVRFFFPAIFLFVAAGAGF
                     AGASSGLDVDLLSLPGPLLCVVVLLVFRSLLRVRTEEGARLNESIEGLRMFITAAEKH
                     RLEMMNSPAETPRLFETLLPYAFALDAAETWANRFQSVLAAANYTPSWYRGDLNTFTT
                     AAGVAAFTSGFSGAVSSGTRSSGSGGSGSSGGGGGGGGGRGW"
BASE COUNT          305 a          482 c          461 g          238 t
ORIGIN      
        1 gccggctacc aacccctgcc gcctccgccg cctccgccgc cgcccgatga gccgcttccg
       61 ccgctgccgg acgaccgtgt ccccgaggaa acggctccgg aaaatcctga cgtgaacgcc
      121 gctactcccg cggcggtggt aaaggtattc aggtcgcccc ggtaccagga aggcgtgtaa
      181 ttggccgcgg caagcacgct ctggaaccgg ttcgcccacg tctccgccgc atccagggca
      241 aaagcgtagg gaaggagggt ctcgaagagc cggggcgttt cagcggggga gttcatcatc
      301 tcgaggcggt gcttctccgc cgccgtgatg aacatccgca gcccttctat gctttcgttg
      361 agcctggccc cttcttccgt ccggaccctc aggagagacc tgaacaccag cagcaccacc
      421 acgcacagga gcggtcccgg aagagacagg aggtccacgt caaggccgct cgacgctccc
      481 gcgaaaccgg cgccggccgc gacaaacagg aagatggcgg gaaaaaagaa ccggacgaag
      541 aatttcgcga taaagccgcc ctttccggag ggaaggggca atgagagagg aagaagcagg
      601 aatgggccgg agatcagcgc aaaaatgggt tcgaagcggg gttgtcccga gagaatcatg
      661 agaatgagca taagcactgc gtacaattca aaaagaaccc cgccggcaat ccattttccg
      721 agattggtct tcagcagcgg cctgccccgt tcgcggaagc cgagaccgag acggcggaat
      781 gcatcctgga tgaccggcct gtcggcgctc gaaaggtgaa tttccgtccg tgtggaaccg
      841 aagagatcgt cggagatcct ccgttcgtct tccgtcagct ctgtccccgc aagcttctcc
      901 ctgttcagct tcaaccggta ggacgtcccc gcaagttttg aaaggagctt cagggccgtt
      961 cccgcccccg agggcagcgt cccggcccgt ttcgccgctt ctcccgcgag ggtgctctcc
     1021 tctatcgcaa gcacaccctt gacggccatg ccgaggatca tggcggcgaa ggtcttgtcg
     1081 tccgccgtca tggtgcggac ataccgggca aacccagcct ccacaccggc aggaggtgag
     1141 aaaagaggga tgaccggcct gggtgcgggg tccttccccc accggatcca gaaaaggagc
     1201 atcaccgcca ggaggacgac cggcatggcg aggtggaccg ccctgtaggg ggaacggctc
     1261 caccgttcca gaatgggcgc agcctccgaa ggctgggcga tgattccctt gggccaggtg
     1321 tacactacgg tgagcccctc tcccggtgca aagggaacag cggattccac ggagccgtcg
     1381 ggaagcggcc gggcatcctg tcccttctct cccgtccgtc cggtatagaa ctccacggcc
     1441 gaaaacccct cgccgaattc ccttccgggg agcttcgccc gaaagg
//
LOCUS       DAOTHD010000628         1485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_101925_length_1485_cov_3.229371, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000628 DAOTHD010000000
VERSION     DAOTHD010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101925_length_1485_cov_3.229371"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="P5219_09895"
     CDS             <1..1287
                     /locus_tag="P5219_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27116.1"
                     /translation="GSAAEVGKLWGETNRESILKAFNAFLSRADGKEEQLRSFAKLSI
                     DLSQKIGCSYWIDELNAIADTAGIDRELYIAFTFGRYRDLALLYKGVGCTTFAVTPPA
                     TKNGRIIFHKTRETAPDLQSGYLKRITGVQGTDRQPYKFFGEMGTADTGISFFVNEKG
                     LAGGADVPSQWQNRECYVGSYKGTLPFVTPPKYDGFMNHYVPRYIAEHCRDVDEAKET
                     LHSFAEKGYLASGKWGTNYLFVDARGKILQIADDCYKIIEEQMDPALEKDGKSWKGIY
                     FTVHRENDYGTPEDALVSAYGKITAELVNSPGVAKHPAMWNFPRAQSSATIVIDPEFP
                     ETLTTIFVTLPAYSYSIPFFMGANATPKALADGTVYSVQKDSYRYSEFYEAGINDLWR
                     AFAYDIRERVKKGEDVTKDLNGFFGQMVDQVLTMNK"
BASE COUNT          368 a          436 c          405 g          276 t
ORIGIN      
        1 ggaagcgcag cggaagtggg aaaactctgg ggagagacga acagagaatc gatcttaaag
       61 gctttcaacg cgtttctttc ccgggccgac ggcaaggaag aacaattgag gagcttcgcg
      121 aagctctcca ttgatctctc ccaaaagatc ggatgctcct actggatcga tgagctgaac
      181 gccattgcag ataccgccgg catcgaccgg gaactctaca tcgccttcac cttcggaagg
      241 tacagagatc ttgctctcct gtacaagggt gtgggatgca cgacctttgc cgtcacccca
      301 ccggcaacga aaaacggccg gatcattttt cacaagaccc gagagacggc ccctgacctc
      361 cagtcaggct acctcaaacg gatcaccgga gtccaaggaa cggacaggca gccctacaag
      421 tttttcggtg agatgggcac cgcggacacc ggcatttcct tcttcgtcaa cgaaaagggc
      481 cttgccggag gcgccgacgt cccctcccag tggcagaaca gggaatgcta cgtcggctcc
      541 tacaagggga cccttccctt cgtcaccccg ccaaaatatg acggattcat gaaccactat
      601 gttccccgct acattgccga gcactgcagg gacgttgatg aagcgaaaga aaccctacat
      661 tcttttgcgg aaaagggata cctcgccagc gggaaatggg gcacgaacta cctgttcgtg
      721 gatgcaaggg gaaaaatcct ccagattgca gacgactgct acaagatcat cgaggagcag
      781 atggacccgg ctctggagaa ggacggaaaa tcctggaagg gaatttactt caccgtccac
      841 agggagaacg actacggtac ccctgaggat gcgcttgtat cagcatacgg caagataacc
      901 gctgagcttg taaactctcc aggggtggca aaacaccccg ccatgtggaa tttcccgagg
      961 gcgcagtcca gcgccaccat cgtcatcgac cccgagttcc ccgaaacgct gaccaccatc
     1021 ttcgtgaccc ttccggccta cagctactcc atccccttct tcatgggggc gaatgccaca
     1081 ccgaaagccc ttgccgacgg gacggtatac tcggtccaaa aggacagcta caggtacagc
     1141 gaattctacg aggcggggat aaacgacctg tggagagcct tcgcctacga catcagggag
     1201 agggtcaaga agggcgagga tgtgacgaag gatctgaatg gtttcttcgg ccagatggtg
     1261 gaccaggtcc tcacaatgaa caaatagccc agataggcgc gaagcggctc tccctgccga
     1321 agggggagcc gcttcaaggt cttccgtgcc cttctatttt accgtgaact cgatggcccc
     1381 cagggccccg gcgtcataaa cgtaggatcc gggttttccg gggagcatgt tcccaagggc
     1441 tcccgtcagg agcacatccc ccttctccag agtccagcca tgctc
//
LOCUS       DAOTHD010000629         1484 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_102046_length_1484_cov_2.156053, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000629 DAOTHD010000000
VERSION     DAOTHD010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102046_length_1484_cov_2.156053"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="P5219_09900"
     CDS             <1..610
                     /locus_tag="P5219_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRX27117.1"
                     /translation="GVALPTLNKYTFLLDVGATVRSKPENLFQFAHMGNVYSKKILGV
                     SEPQVALLANGSEEIKGDEVIAGAKALIKASALNFTGYVEGDDIPYSRADVVVCDGFS
                     GNVVLKFAEGLMSAVYSILKEEMGHRIMPKFGMVFMIPMMKDLWARFNYEKHGGTPLL
                     GVNGIVVKAHGRSKAPAITNALRVARNFVLQNGVELIREELG"
     gene            625..>1484
                     /locus_tag="P5219_09905"
     CDS             625..>1484
                     /locus_tag="P5219_09905"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRX27118.1"
                     /translation="MGFFSGITVSIKGTGMYVPEKVLTNNDLSQMVETSDEWIRERTG
                     ICRRHIAAEGELTSEVAAKAARNALENSGTDPLELDMIIVATNSPDTLFPGVGPKVQG
                     ILGAEKAGAFDVQSGCTSGVYALSLAAGGISSGIWKKVLVIGAEVLSPLIDWTDRNTC
                     ILFGDGAGAMVLGVSAEEEGRFISADLRSDGTRHDQITLSGGLVEHPASHETLENKLH
                     FVSMKGNDVFRFVNRIIPPFLRQFCLDSGMEPEEITWWFFHQANQRIIERSAERLGIS
                     PEKVVINIDE"
BASE COUNT          355 a          385 c          431 g          313 t
ORIGIN      
        1 tggtgtggcg ctgccaaccc tcaacaagta caccttcctg ctcgacgtgg gggcgactgt
       61 acggagcaag cccgaaaacc tgttccagtt cgcccatatg ggcaacgtgt attcaaaaaa
      121 aatactcggc gtttcagaac cccaggttgc ccttcttgcc aacggatccg aggagatcaa
      181 gggtgacgaa gtcattgcag gagcgaaagc tctcataaag gccagcgccc tgaattttac
      241 aggatatgtt gagggtgacg acattcccta tagccgggca gacgtggtgg tatgcgacgg
      301 cttttcggga aacgtggtgc tcaagtttgc ggaaggcctc atgtcggctg tatattccat
      361 actcaaggaa gagatggggc acagaataat gcccaagttc ggtatggttt tcatgatccc
      421 gatgatgaag gacctctggg cgcggttcaa ttacgaaaaa cacggaggaa cgcccctcct
      481 tggagtgaac gggatcgttg tgaaagctca cgggcgatcg aaggccccgg caatcacaaa
      541 cgccctgaga gtcgcacgga atttcgttct gcagaacggt gtcgaactta taagagaaga
      601 actggggtaa aggaggtttc ccctatgggt tttttttccg gtattaccgt ttccataaag
      661 ggcacgggaa tgtacgtccc cgaaaaggtg ctgacaaaca acgatctttc gcagatggtg
      721 gaaaccagcg acgaatggat ccgggagaga acgggcatct gccgccgcca cattgccgcg
      781 gagggggaac tgacaagcga ggtggctgcg aaagccgccc gaaacgccct cgagaattcc
      841 ggcaccgatc ctctggagct ggatatgatt atcgttgcga cgaacagccc tgataccctc
      901 ttccccggag tgggccccaa ggtccaggga atcctcggag cagaaaaggc gggcgccttt
      961 gacgtccagt cggggtgcac cagcggtgtg tacgcccttt ccctggccgc cggcggaatt
     1021 tcgtcgggga tctggaaaaa agtcctcgtt atcggagcgg aagtcctttc tcccctgatc
     1081 gactggaccg acagaaacac gtgcattctc ttcggcgacg gtgcgggggc catggtcctg
     1141 ggggtgtcgg cagaagagga aggccgtttc atttcggcag acctgaggtc agacggaaca
     1201 cggcacgacc agatcacctt gtccggcgga ctcgtggaac accctgcatc ccatgagacc
     1261 ctagaaaaca agcttcattt cgtctcaatg aaaggaaacg atgttttccg tttcgtcaac
     1321 aggatcattc cgcccttttt gcgccagttt tgccttgatt caggcatgga gccggaagag
     1381 atcacctggt ggtttttcca ccaggccaat cagaggatta tcgaacggtc agcggagcgt
     1441 cttgggattt ctcccgagaa agtcgtcatc aacatcgacg aata
//
LOCUS       DAOTHD010000630         1482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_102180_length_1482_cov_3.272600, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000630 DAOTHD010000000
VERSION     DAOTHD010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102180_length_1482_cov_3.272600"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="P5219_09910"
     CDS             complement(<1..1147)
                     /locus_tag="P5219_09910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRX27119.1"
                     /translation="MYARIEELAGRMTEKLVSCRRDFHKYAESGWFEMRTASLIARRL
                     TDMGYEVLAGRDVCQDESRMGLPSDEELEKNCIRAKEQGADTEYLEKVKGGFTGVMGI
                     LRCGEGPTVALRFDIDALGVVEDASPEHRPFREGFSSVNPGMMHACGHDGHAAIGLGV
                     AEILMAEKDGLRGTVKIIFQPAEEGVRGAKSIVDKGHLDGVDYVLACHVTGLETDEDR
                     SSDITAGSGGSLATSKMDAFYFGKSAHAGSNPQDGRNALAAAVTAVQNLLAIPRNSKS
                     DTRVNVGTLQAGSGRNVIPEFAKMELEVRGKTSEGNIYMEQWARRILRTAAEMHDCTL
                     EVKKMGEAFLLESDQGLMELIRDVCEKNLQTVRVHPRRGGAADDLCGF"
BASE COUNT          284 a          457 c          411 g          330 t
ORIGIN      
        1 cgaaaccgca cagatcatcg gcggccccgc cgcgccgggg atggacccgg accgtttgca
       61 gattcttttc gcatacatcc cggatgagtt ccatgaggcc ctggtcgctt tcaagcagga
      121 aagcctctcc catcttcttt acctcaagag tgcagtcatg catttcggcg gccgtccgga
      181 ggatccgccg ggcccactgc tccatgtaga tgtttccctc gctggtcttt ccccgaactt
      241 ccagctccat tttggcgaat tcggggatga cgttccgacc cgacccggcc tgaagggtac
      301 ccacgttgac ccgggtgtcg gacttgctgt tccgggggat ggccagcagg ttctggacgg
      361 cggtgacggc agctgcaagg gcgttccgcc catcctgggg gttgcttccg gcgtgggcgg
      421 atttgccgaa atagaatgca tccattttgg acgtggccag ggaaccgccg gaacctgcgg
      481 tgatgtccga cgacctgtcc tcgtcggttt ccagcccggt gacatggcag gcgaggacgt
      541 agtccactcc gtcgaggtgc cccttgtcca caatgctttt ggcaccccgc actccttcct
      601 cggctggctg gaaaatgatt ttgacggtac cccgcagccc gtctttctcc gccataagga
      661 tctctgccac gccgaggccg atggctgcat ggccgtcatg gccgcaggca tgcatcatgc
      721 cgggattcac ggaactgaag ccctcccgga acggacgatg ttccggggag gcgtcttcca
      781 ccacgcccag ggcgtcgatg tcgaaccgga gggccaccgt tggcccttcc ccgcagcgga
      841 gtatacccat aacacctgtg aagccgccct tgaccttttc gagatactcc gtatccgcac
      901 cctgctcctt cgcccggatg cagttcttct ccagctcttc atcagagggc agtcccatgc
      961 gggattcgtc ctggcagacg tcccggcctg ctaaaacctc ataccccatg tcggtgagac
     1021 gcctggcgat gagagaggcc gtcctcatct cgaaccagcc gctttccgca tacttgtgaa
     1081 aatccctgcg gcaggagacc agcttctccg tcatcctgcc cgccagctct tctatacgtg
     1141 catacatagg catccctccg gctgatcatt tctaccccgt attatggaga agggagggcg
     1201 tcttgtccaa tgcactatcc ctaaaagatg actttttccc gatattttcc caaaaagctg
     1261 tatactggga ttgacggaag cagaaatgcc atgaggaaga aagaacgggg cggaacacaa
     1321 ccgccgcccg gggcaggtga ggtttttgaa agattcaagg ggctctgcag ggaagaaaaa
     1381 agaaaggctc tgcttcctga tcgcggatat tgccgcgatg atcctgtttt ccacagcttt
     1441 gtgcatgtgt atcgagattt tcatcgcgaa gctgaccttc tt
//
LOCUS       DAOTHD010000631         1482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_102193_length_1482_cov_2.861948, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000631 DAOTHD010000000
VERSION     DAOTHD010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102193_length_1482_cov_2.861948"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="P5219_09915"
     CDS             complement(<1..400)
                     /locus_tag="P5219_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HRX27120.1"
                     /translation="MNRRYRAVLCGYYGFGNLGDELLASALVEAFEKIGVPRDELAIL
                     SASPADTERSLSLRAVNRWKTTEVFRLLRESDTLLLGGGGLFQDVTSVRSSAYYWGMI
                     RLASLAGALPWAVGQSVGPFRTLSGMFFRPG"
     gene            complement(397..>1482)
                     /locus_tag="P5219_09920"
     CDS             complement(397..>1482)
                     /locus_tag="P5219_09920"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HRX27121.1"
                     /translation="LLRAVKERSVRLLVFRPSAMESSADPYGTFLGELGNLAAGLSAH
                     GIAVSWPEPFSPWRTGLVGAAALALAFVYSLLQYLRRFFLFSGADDHGRTGRVSRKGA
                     LLLGAVVLVTALAVRFVPPAARIIGALAAVFVVTEASFLALDGWRRPWAGLVGSFLFA
                     VAGGLAIAAFFSEPAYMLRLRAFSGVKATLFLPPLLVLLADLRRREHPESLGDIFRRP
                     PLWGELFLLGILLAGAGLVLFRSDNVQFVPAFEVRMREFLERILVARPRNKEIFLGYP
                     ALLLWYYVRKADLWPRYREVLRMATVLGFSSAVNSFCHFHTPLYFILFRQFNGLWTGV
                     LVGMMGIVLLRFLILPLWEKYGRMVTE"
BASE COUNT          342 a          470 c          488 g          182 t
ORIGIN      
        1 gccctgggcg gaagaacatc ccggagaggg tccggaacgg tcccaccgac tgtcctaccg
       61 cccagggcag tgcccccgca agggacgcga gacggatcat tccccagtaa taggcggacg
      121 accggacgct cgtgacgtcc tgaaagagcc cccccccgcc gagaagcagg gtgtcgctct
      181 ccctgaggag gcggaaaact tccgtcgttt tccatcggtt caccgcccgc agagacagag
      241 accgctcggt atccgcagga gacgctgaga gtatggccag ctcatccctc ggaacgccga
      301 ttttttcgaa cgcctccacc agagccgagg caagaagctc gtccccgagg ttgccgaacc
      361 cgtagtagcc gcaaagcacc gcgcggtagc gacggttcac tccgtcacca tcctcccgta
      421 cttctcccac aggggcagaa tgaggaacct gagaagcacg attcccatca tacccacgag
      481 gacgccggtc cacaatccgt taaattgcct gaagagaatg aaatagagag gcgtatggaa
      541 atgacagaag ctgttcaccg ccgacgaaaa accgaggacg gtggccatgc ggagcacttc
      601 cctgtaccgg ggccacaggt cggccttgcg gacgtaatac cagaggagga gcgccgggta
      661 cccgaggaag atctccttgt tcctgggccg ggccacgagg atgcgctcaa ggaactccct
      721 catgcggacc tcaaaggcag gcacgaactg gacgttgtcg ctgcggaaga gaaccaggcc
      781 cgctcccgcc agcagaatgc cgaggagaaa aagctccccc cagagagggg gacggcggaa
      841 gatatctccc agggactcgg gatgttccct ccgcctcagg tcggcgagca gcaccaggag
      901 cggcggaaga aagagcgtcg ccttcacgcc ggagaaagcc cggaggcgga gcatgtaggc
      961 gggctcgctg aagaaggcgg cgatggccag cccccccgcc acggcgaaga ggaaacttcc
     1021 cacgagcccc gcccaggggc gcctccaccc gtcgagggcg aggaaagacg cctccgtaac
     1081 aacaaacacc gccgcgaggg ccccgatgat cctggccgcc ggcggaacga aacggacagc
     1141 gagagccgtg acgaggacga ctgccccgag aagaagggct cctttccggg aaaccctgcc
     1201 cgtgcgcccg tggtcgtctg cgccggagaa aaggaaaaac cgccggagat actggaggag
     1261 ggagtagaca aaggcgaggg ccagggccgc agcgccgacc agtcccgtcc tccagggcga
     1321 gaagggttcg ggccaggaaa cggctatgcc gtgggccgaa agccccgccg cgaggttccc
     1381 aagctccccg aggaaggtgc cgtaggggtc ggcggaggat tccatggcgg agggacggaa
     1441 gacgagcagg cggacggacc gttccttgac cgcccggaga ag
//
LOCUS       DAOTHD010000632         1479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_102514_length_1479_cov_2.440309, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000632 DAOTHD010000000
VERSION     DAOTHD010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102514_length_1479_cov_2.440309"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="P5219_09925"
     CDS             <1..465
                     /locus_tag="P5219_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRX27122.1"
                     /translation="LFGLGRGQSREDHRRIAGERPALFRPILDRSAPLGEPAAALTEA
                     LLPASGKWSPTFRRAFRETLEASFAAHAEGIREQQTALAECPSLEGRLASFAMPVLVV
                     EGSEDRLIPPGEGEILAGGIPGALLRILSGGHGLVYEAPGELAVAVAEFLGR"
     gene            complement(490..1278)
                     /locus_tag="P5219_09930"
     CDS             complement(490..1278)
                     /locus_tag="P5219_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27123.1"
                     /translation="MNLNRTALAALLLFLLPAFPARAGEHPFWAADRRENFRILLPGG
                     WTVRRHLSQELALRARSRDGTAYLEVHVLREPFPTEAEAADGFGDRDDFLSLSPVEFW
                     RGRIVSVGGEEGIVRRYKVSVDGRPAEASAACFGRDGTWFLVLAVVSSDRAGIYASDL
                     EQSLRSLTLLTAPSAPGDPIEGRWRWHNGRILTLGRHGRVAPSSLVSSWRRKPPTPEE
                     AAGAYLLSIPGPAGGEEEAVLSPDGKKLFRVSGGERIQIAARLE"
BASE COUNT          259 a          458 c          479 g          283 t
ORIGIN      
        1 ctctttggcc tcggccgtgg gcagagccga gaagaccacc ggcggatcgc cggggagcgg
       61 cccgctctgt tccggccaat tctcgacagg agcgctcctt tgggggagcc ggccgctgcc
      121 ctcacggagg ctctgcttcc ggcttccgga aaatggagcc cgacgttccg cagggccttc
      181 cgggagaccc tagaggcctc cttcgccgcc catgctgaag gaatccggga acagcagacc
      241 gccctggcgg aatgtccgtc cctggagggc aggcttgctt cgttcgccat gcccgtcctg
      301 gtggtagaag gctcggagga ccggctgatt cccccgggtg agggggagat cctcgcaggg
      361 gggattccgg gggccctgct gaggattctt tcgggaggac acgggctggt ctacgaggcc
      421 cccggggaac ttgccgtggc ggtggcggag tttctcggcc ggtaggatct ttctctttcg
      481 ccgtcctttt tattccagcc gtgcggcgat ctgtatcctt tcgccccctg agacccggaa
      541 aagcttcttc ccgtcggggc tgaggaccgc ttcctcttct ccgcccgccg gaccgggaat
      601 ggaaaggaga taggcaccgg cggcctcttc cggcgtagga ggcttccttc tccaggagct
      661 taccagggat gacggagcga cccttccgtg cctcccgagg gtgagaatgc gtccgttgtg
      721 ccacctccac cggccttcga tggggtcccc gggagcggaa ggcgccgtca ggagagtaag
      781 gctcctgaga ctctgttcaa ggtcggaggc gtaaatacct gccctatctg aggagaccac
      841 ggcgagaaca aggaaccatg tcccgtcccg gccgaaacag gcggccgacg cctccgcggg
      901 ccggccgtca acgctcactt tgtatctcct cacaatccct tcctctccgc cgactgaaac
      961 aattctccct ctccagaact cgacgggaga gagggagaga aaatcatccc ggtccccgaa
     1021 gccgtctgcg gcttcagctt ccgtcggaaa aggttccctg aggacatgca cctcaagata
     1081 tgctgttccg tcccgggagc gggccctcag ggccagttcc tgtgacaggt gccgcctcac
     1141 cgtccatccg cccggaagga ggatgcggaa attttccctc ctgtcagcag cccagaatgg
     1201 atgctccccg gcccgggccg ggaaagccgg cagaaggaag agcagcaggg cggcaagggc
     1261 agtccggttc aggttcatgg gtttctcctt cctgtttttt tgcagtgctg tttgagtatc
     1321 ggccttcggg cggaaaggaa aaaggcggac cggagtccgc cttcgcttca ggggagaatg
     1381 gcgaagaccc gggcggggaa gcccgagccg aagaagggct tggggaaagt cctctttcat
     1441 cttcgtaata cctttgaaac aaatgcggag ggcctgttg
//
LOCUS       DAOTHD010000633         1476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_102769_length_1476_cov_3.239972, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000633 DAOTHD010000000
VERSION     DAOTHD010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102769_length_1476_cov_3.239972"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="P5219_09935"
     CDS             complement(<1..1245)
                     /locus_tag="P5219_09935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRX27124.1"
                     /translation="MRRTRSRLSLPARKGRFDPFRRTFIFFLLLAAGAVAVLSVLDGR
                     AVARQEEIVNDQQFLSATLARLAAEDRFSSVDASAGFLLNHVFSRTLDGTMTVAEAQN
                     YLQPMVYNMPEVLGFYFLPNVPGKDFPPVTAFRSTPAGNDGRVFAASTADRLGPGLFS
                     QDDAVALSGSVMVTPQQRMALFCHGFSGGGSLRGVLLTVVDLSAVLSRYVVPTARGRS
                     GFSMVLSGDGTPVWFNDPSQRGPEGSFGRESPELESVRSLVSGAVSGKSSLEIPGAKG
                     GSRLLVAWNTLRMGGKRLAVVLASPAEEAEAALSGLRTQRNLSVGSLLLLVVLGSAFF
                     TGKQRQEEIKRREAGFRAVFDNASSGIAILSVEGRFLSCNATWETMTGLSQERLRQKT
                     IFDLAAPGQGRDELGRILGENGG"
BASE COUNT          316 a          441 c          442 g          277 t
ORIGIN      
        1 tcccccgttc tctccaagga tccggccgag ttcatcccgt ccctggccgg gagcggcaag
       61 gtcaaaaatg gttttctgcc gcagccgttc ctgcgaaagg cccgtcatgg tctcccaggt
      121 ggcgttgcat gagagaaatc tcccttcgac gctgaggatg gcgattccgg aggaggcatt
      181 gtcgaaaacg gccctgaaac cggcttccct tctttttatc tcctcctgcc gctgttttcc
      241 cgtgaaaaag gcgctcccca gaacgacgag aaggaggagg gagcccacag acaggtttct
      301 ctgggttctc aggccgctga gagccgcttc cgcttcttcc gcgggagaag ccagcaccac
      361 ggcgagacgc tttccaccca tccggagggt gttccatgcc acgagcagtc tgctgccgcc
      421 cttagcgccc ggtatttcca ggctgctttt tccggaaacg gccccggaaa cgaggctccg
      481 gacactctcc agttcagggg attccctgcc gaagctccct tcggggccgc gctgcgaagg
      541 atcgttgaac cacaccgggg tgccgtctcc ggaaagcacc atggaaaatc cgctccttcc
      601 cctggccgtg ggaacgacgt accgggacag caccgcagag aggtccacca ccgtgagaag
      661 cactccccga agggagcctc ctccggaaaa tccgtggcag aaaagggcca tccgctgctg
      721 gggtgtcacc atcacggatc cggaaagggc cacggcatca tcctgggaaa agaggccggg
      781 accgaggcgg tccgccgtcg aggcggcgaa gacccggccg tcatttcccg ctggcgtgga
      841 acggaaggcc gtgaccggcg gaaaatcctt tcccggtacg ttgggaagga aataaaagcc
      901 gagtacctcc ggcatgttgt ataccatggg ctgaagatag ttctgggctt ccgccacggt
      961 catggttccg tcgagggtac gggaaaagac gtggttcaga aggaacccgg cagaggcgtc
     1021 gacagaggaa aaccggtcct cggcggcaag gcgcgcgagg gtcgcggaga ggaactgctg
     1081 gtcgttcaca atttcttcct gccgggcaac cgcacgaccg tcaaggaccg acagaacggc
     1141 tactgctccg gcagccagaa ggaggaaaaa aataaaagtg cgcctgaacg gatcgaaacg
     1201 ccccttccgc gccggaaggg acaatcggct gcgtgttcgt ctcaagaccc tgttctcctg
     1261 attcttttgg atagatgcaa tgggtacatt atatcaaaaa aaaagcctat tcccttttac
     1321 ggcaccttat gccctctttt cttttcgccc gcaggatgct aacatttcgt tatcacacaa
     1381 cgggcggagg ttgtcatgga gactttggga aagctcgcgg tgctcgccga aggagcgaaa
     1441 tacgacgtgt cctgctcgtc cagcggaagc gccggg
//
LOCUS       DAOTHD010000634         1476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_102801_length_1476_cov_2.308234, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000634 DAOTHD010000000
VERSION     DAOTHD010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102801_length_1476_cov_2.308234"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="P5219_09940"
     CDS             <1..823
                     /locus_tag="P5219_09940"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRX27125.1"
                     /translation="LVIAVLPLLENTFDVLSPLRLMELSHPSNPLLKKLQIEAPGTYH
                     HCLMLGTLAEVVADKLGMNSNLLKAGAYFHDIGKLRRPQFFVENQMGNENIHDELKPS
                     LSALVIIAHIREGLELAEEYHLPEMIRAFIAEHHGTTCLSYFYRKARSMGLSVPRDQF
                     CYPGPRPRTRETGLLMLVDSIEAAVRAEMRASTSVPDLEKTIEGVVEAKMSEGQLDDV
                     DFTIRDLAVIRQTLLYAFQSMYHTRKVKEIQDKDQLEQKLKKQEEESIEGSLASR"
     gene            798..1322
                     /gene="ybeY"
                     /locus_tag="P5219_09945"
     CDS             798..1322
                     /gene="ybeY"
                     /locus_tag="P5219_09945"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRX27126.1"
                     /translation="MKVHLRLDNSEDADPASRAPLGDMLSAADGVLSAALEKLARPGT
                     LPPAAEISVTLLSLEEMEEQNRLHRGLPEPTDVLSFPLWEEDGEFRPEKGMPVLPLGD
                     IVICPDYVRKNTPTEQAFLEEMALMIAHGFLHLLAWDHDTDERRTAMEAAQETIRRAL
                     LDAAAESGKGGVEQ"
BASE COUNT          315 a          469 c          421 g          271 t
ORIGIN      
        1 cctggtcatc gcagtgctcc ccctgctgga gaacaccttt gacgtcctct cgcccctccg
       61 cctcatggag ctgagccatc cgtcgaaccc cctcctgaag aagctccaga tagaggcccc
      121 ggggacctac catcactgcc tcatgctcgg gaccctggca gaggtggtgg cggacaagct
      181 cggcatgaac tcgaacctgc tcaaggccgg ggcatatttt cacgacatcg gcaagctccg
      241 gcggccccag tttttcgtgg agaaccagat gggcaacgaa aacatccatg acgagctcaa
      301 gccgtccctt tctgccctgg tgatcatcgc ccacatccgg gaagggctgg aactggcgga
      361 agagtaccac ctgcccgaga tgatccgcgc cttcatcgcg gaacaccacg gcaccacctg
      421 cctgtcctat ttttacagga aggcccggag catggggctc tcggtccccc gggaccagtt
      481 ctgctatccc ggcccccgtc cccggacgag ggaaacgggg ctgctgatgc tcgtggactc
      541 catcgaggcg gcggtgaggg ctgagatgag ggcgtccaca tcggtgcccg acctggagaa
      601 aaccatcgag ggcgtggtgg aggccaagat gtcggaagga cagcttgacg acgtggattt
      661 caccatccgc gaccttgcgg tgatacggca gacgctgctg tacgccttcc agtccatgta
      721 ccatacgagg aaggtcaagg aaatacagga caaggaccag ctcgaacaga aactgaaaaa
      781 acaggaggaa gaatcaattg aaggttcact tgcgtctcga taacagcgaa gacgccgatc
      841 ccgcaagtag agcccccctg ggggacatgc tttcagcagc ggacggcgtt ctctcggccg
      901 ccctggagaa actcgcccga cccggaaccc ttccgccggc ggcggaaatt tcggtcaccc
      961 ttctctccct cgaggaaatg gaagagcaga atcgcctcca caggggactt cccgaaccta
     1021 cggatgtcct ttcctttccc ctgtgggagg aagacggaga gttccgccct gaaaagggaa
     1081 tgccggttct cccccttggg gacatcgtca tctgccccga ctatgtccgg aagaacaccc
     1141 ccactgaaca ggccttcctt gaagagatgg ccctcatgat tgcccacggc tttcttcatc
     1201 tcctggcgtg ggaccatgac accgacgaac ggcgaaccgc catggaagcc gcccaggaaa
     1261 cgatccgccg tgccctcctc gacgccgccg cggagtccgg caaaggggga gtggagcagt
     1321 gaacgtgatg attacagaat cgatcctcct gctgatcctg ctgcttgtcc tctctgcgtt
     1381 tttcagtgcc tccgagacgg ccatcacgtc ttccggccgg ggaaagatcc tggccctcat
     1441 ggagcgctat ccataccaga gacggttttt cgaatg
//
LOCUS       DAOTHD010000635         1476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_102807_length_1476_cov_2.214638, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000635 DAOTHD010000000
VERSION     DAOTHD010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102807_length_1476_cov_2.214638"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="P5219_09950"
     CDS             complement(<1..1017)
                     /locus_tag="P5219_09950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRX27127.1"
                     /translation="MGILKKANVWIWIIALAAGLGVLGYKVVMPSGAQQKPPAPPAGI
                     TVTVYEVQPRIWEIWRSFYGTVRSAQVQRVNSNTREVIEDIAVEVGDHVKKGDILISL
                     ASQSQAAVLSARQAAYEDARSRYESLQKLYRAGGTSKQELDRANVQLKDESAKLRDAR
                     TTVSRTQIRSALNGVVVRRSAEKGEFAEPGRELLAVADFDRREVEMLVSPTIRGLIIP
                     GMDVLVKAPWGGETMGKIFRMDPEADAATGFFRAIVRLPAECRLIPGDYVRMEVRMVR
                     RENTIAVPYESILREDGNPFVFVVSGEGASKRPIVTGEGSGGYVEVSEGLSPGEKVVK
                     TGAGS"
     gene            complement(1131..>1476)
                     /locus_tag="P5219_09955"
     CDS             complement(1131..>1476)
                     /locus_tag="P5219_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27128.1"
                     /translation="APHLGETSGLKGVILAILLGASSAGPLYIAFPVAEVMIRKGTSL
                     RNAFIFLGAWSTMRVPMILFEIQNLGAIFALSRYGASLAGVLLMGAFMERVLGPKDRE
                     DILARFAEHRAG"
BASE COUNT          297 a          481 c          415 g          283 t
ORIGIN      
        1 gctgcccgct ccggtcttca cgaccttttc gcccggggag agcccttcgg aaacctccac
       61 gtaccctccg ctcccctcgc cggtgacgat gggccttttg gaagccccct caccggaaac
      121 aacgaagacg aaggggtttc cgtcctcacg gaggattgac tcatagggca ccgcaatggt
      181 attttcccgg cggaccatcc ggacctccat ccggacataa tccccgggaa tcagcctgca
      241 ctcggcaggc aggcggacga tggcccggaa gaatcccgtg gcggcgtcag cctcggggtc
      301 cattcggaaa attttaccca tggtctcccc tccccaggga gccttgacca ggacatccat
      361 tcccgggatg atgagacccc ggatcgtcgg ggaaacaagc atctccactt ccctgcggtc
      421 gaagtcggcc acggcgagaa gttcccgccc cggttcggca aattccccct tttccgcgga
      481 ccgccgcacc accacgccgt tcagggcgga ccggatctgc gtccgggaca cggtggtccg
      541 ggcatcccga agctttgcgg actcgtcctt gagctgcacg ttggcccggt caagctcctg
      601 cttcgaggtt cctccggccc tgtacagctt ctgaagactc tcgtagcggc tcctggcatc
      661 ctcgtaggcc gcctgccggg cgctgaggac agccgcctgg ctctggctgg cgagggaaat
      721 gaggatatct cccttcttca catggtcgcc cacttccacg gcgatatcct cgatgacttc
      781 ccgtgtgttg gagttcaccc tctggacctg ggccgaccgg acggtaccgt aaaagctcct
      841 ccagatctcc cagatccggg gctgcacctc gtagacggta acggtgattc ccgcgggggg
      901 cgcaggaggc ttctgctggg cgcccgaagg catcacgacc ttgtacccga ggactcccag
      961 tcctgcggcg agagctataa tccagatcca cacgtttgct ttcttcagaa ttcccatggg
     1021 gtacctctct tcatgaaccg gagtaaaaaa tacggatttt attctatcct atgaaggagc
     1081 gccggggata caaaaaaata ctgaaaaaac aacacattgc ctttcggaat tcaccccgcc
     1141 cggtgttccg cgaagcgcgc cagaatgtcc tccctgtcct tcggcccgag caccctttcc
     1201 atgaaggccc ccatgagaag aacgccggcg agagaggccc cgtaccggga aagggcaaat
     1261 atcgccccca ggttctgaat ttcaaagaga atcatgggaa cccgcatggt ggaccatgcg
     1321 cccaggaata taaaggcgtt cctgaggctc gtccccttcc ggatcataac ttccgccacg
     1381 ggaaaggcga tgtacagtgg accggcggag cttgccccga gaaggatcgc gaggataacc
     1441 cccttgaggc cggaagtctc tccgagatga ggggcg
//
LOCUS       DAOTHD010000636         1474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_102978_length_1474_cov_2.780832, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000636 DAOTHD010000000
VERSION     DAOTHD010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102978_length_1474_cov_2.780832"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="P5219_09960"
     CDS             complement(<1..1096)
                     /locus_tag="P5219_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HRX27129.1"
                     /translation="MKQIRDDAMKIGLKAIETVLPENAVRAALQGGEFTARLSGGGEI
                     VLVAIGKAAWRMAKAASETLGEKLSGGTVITKYGHSMGPIEGLEIFEAGHPFPDGNTL
                     TATARALEKVKNLSDKDTVLFLVSGGGSALFEKPRDGVSLEDLVSVTDQLLACGADIV
                     EINTIRKRLSSVKGGRFARCVQPARVFAVVLSDVLGDRLDSIASGPAHPDGSTARESL
                     RIVDKYGLSLRPGLLDALGEETPKELENVSTVIAGSVSALCAAAESSAAGLGYRTLLL
                     TTTLNCEAREAGSFLASIARQIRACGTPLVPPCAVVLGGETVVHLKGKGRGGRNQELA
                     LAAAPGIRGLKDTVLLSLGSDGTDGPTDAAG"
     gene            complement(1093..>1474)
                     /locus_tag="P5219_09965"
     CDS             complement(1093..>1474)
                     /locus_tag="P5219_09965"
                     /inference="COORDINATES: protein motif:HMM:NF028352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide-binding domain containing protein"
                     /protein_id="HRX27130.1"
                     /translation="VLLASSLEERQVEAGRNADAGERISAVLADAVSRVLAACGTGGV
                     FVTGGEAAIHVVRALGGRGTSLVGEVEAGIPLVRLLGGPLEGLPLITKAGGFGGPETM
                     ERCIAVLSSCRGKKNSKNEVTAGS"
BASE COUNT          247 a          513 c          458 g          256 t
ORIGIN      
        1 ctcccgccgc gtcggtgggt ccgtcggtac catcggaccc cagggagaga agaacggtgt
       61 ccttcagccc cctgattccc ggcgcggccg ccagggccag ttcctggttc cggcctcctc
      121 tgcccttccc cttgagatgc actaccgtct cccctccaag aaccacggcg catggaggca
      181 ccaggggggt gccgcaggcc cggatctgcc gggcgatgga ggccagaaaa gagcccgctt
      241 cccgggcctc gcagttcagg gtggtggtga gcagcagcgt ccggtacccc agtccggcgg
      301 cggatgattc cgcggctgcg cagagggcgg agacgcttcc cgcaatcacc gtggaaacat
      361 tttccagttc cttcggcgtc tcctccccga gggcatcgag aagtcccggc cgcagagaga
      421 gaccgtactt gtccacgatc cggagtgact cccgcgccgt ggatccgtcg ggatgggccg
      481 gtccggaagc gatgctgtcg aggcggtctc ccagcacgtc ggagagcacc acggcaaaaa
      541 cacgggcggg ctgtacacaa cgggcgaatc tgcccccctt gacggaggag aggcgcttcc
      601 ggatcgtgtt gatctccacg atatcggcgc cgcaggcaag gagctggtcc gtcaccgaca
      661 ccagatcctc cagggacacg ccgtcccggg gcttttcaaa cagggcggag cctccccccg
      721 acacaaggaa aagcactgtg tccttatccg agagattctt cactttctcc agtgccctgg
      781 ccgtggcggt gagggtgttt ccgtcgggga aaggatgccc cgcttcgaag atttcaaggc
      841 cttctatggg gcccatggag tggccgtact tggtgatcac cgttccgccc gagagcttct
      901 ccccgagcgt ttcggatgcg gccttcgcca tccgccacgc agcttttccg atggcgacga
      961 ggacaatctc ccctccgccg gagagcctgg cggtgaattc cccgccctga agggcggctc
     1021 gtaccgcgtt ttccggcagg accgtctcta tggccttcag gccgatcttc atggcgtcat
     1081 cccgaatctg tttcatgatc cagcggtcac ctcgtttttc gaatttttct ttccccggca
     1141 ggaggacagg acggcgatgc agcgctccat ggtctccggc ccaccgaatc ccccggcctt
     1201 ggtgatcagg ggaagcccct ccagggggcc tccaaggagc cgcaccaggg gaatgcccgc
     1261 ctccacttct cccaccaggg aggtaccccg cccgccgagg gcccggacca catgaatggc
     1321 ggcctcgccg ccggtgacga agacaccgcc tgtcccgcac gccgccagga cgcgggaaac
     1381 cgcgtccgcc agcacggcgg agattcgctc ccctgcgtcg gcgtttcggc ccgcctccac
     1441 ctgccgttcc tccagggagg aagccaggag gaca
//
LOCUS       DAOTHD010000637         1473 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_103062_length_1473_cov_3.275740, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000637 DAOTHD010000000
VERSION     DAOTHD010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103062_length_1473_cov_3.275740"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="P5219_09970"
     CDS             <1..478
                     /locus_tag="P5219_09970"
                     /inference="COORDINATES: protein motif:HMM:NF017833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HRX27131.1"
                     /translation="PVSIPGVSTVFGAAILLIGISRLMGRNLWLPGRIARKTLPAENI
                     RNAIRRGSRWLSSLEKISRPHRMSGFVTAPMADRINSLAMIAGAVLLMAPFGLVPFSN
                     TLPALALLFLAIGLLQKDGFHILCGHLLNVATVVYFTVLVVGGGVAVRELFRRLAG"
     gene            716..>1473
                     /locus_tag="P5219_09975"
     CDS             716..>1473
                     /locus_tag="P5219_09975"
                     /inference="COORDINATES: protein motif:HMM:NF013178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HRX27132.1"
                     /translation="MNDCFLVLEDGECFGGISFGCRAPKAGELGFPPAGGAGAGEVVF
                     NTAMGGYHEMLTDPSYAGQIVALTCPHGGNYGCSDDWSERGPDEPGLPEIKLSGLVVR
                     SLYSGPVPKGRIPLSEFLRRNGVPGIEGVDTRALTLRLRDRGSCAGLLVSSSSGSALS
                     ASEMDRALAFLKAVPSMEGRNLLPSVGTRSLKCRDVPGNRRFAVVDCGIKGNILRELS
                     LRGVSSTVFPWTATVEEILRSSPDGVLFSNGPGDP"
BASE COUNT          256 a          431 c          478 g          308 t
ORIGIN      
        1 tcccgtgtcc attccggggg tcagtaccgt gttcggggct gcgatcctcc tgatcggcat
       61 cagccgcctc atgggaagga acctgtggct tccggggcgg atagcgagga agacccttcc
      121 agccgagaac atacggaacg ccatcaggcg gggatccagg tggctcagct ctctcgaaaa
      181 aatcagccgg ccccacagga tgagcgggtt cgtgaccgct cccatggctg acaggataaa
      241 cagccttgcc atgatcgcgg gggcggttct gctgatggcg cccttcggtc tcgttccgtt
      301 cagcaatacg ctcccggctc tcgcgctcct gttcctggcc atcgggctgc tccagaagga
      361 cgggttccat atcctctgcg gccacctgct taatgtcgcc accgtggtct atttcacggt
      421 gctcgtcgtc ggcgggggag tggccgtccg ggagctgttc cggaggctcg ccgggtaggc
      481 ccgggcacgg tcccggagaa aaaacgaggc agccgcgtcc atcccacgga tggatgcggc
      541 tgcttttttc atgggcggcc atgctctccc aagtgccggg gcacctcctc agggcacctc
      601 ctcaggattg acaaatttcg tttcctcctg taccgtgagt gcaacaaaag tttatttcat
      661 atatatttat gtactttatt ttatatttgt tggcaggaag gaagatcagg agaaaatgaa
      721 tgactgtttt ctggttctgg aggacgggga atgcttcggg ggaatatcat tcgggtgcag
      781 ggctccgaag gcgggagagc ttggttttcc gccggccggc ggggcaggag cgggagaagt
      841 ggtgttcaac acggccatgg gaggctacca cgagatgctg accgatccgt cctacgccgg
      901 gcagatcgtg gctctcacct gtccccacgg ggggaactac gggtgttccg atgactggtc
      961 ggaacggggc cccgacgagc cgggccttcc cgagatcaag ctttccggcc tggtggtgcg
     1021 gtccctgtac tccgggccgg tgccgaaagg acggataccc ctctccgaat ttctccgaag
     1081 aaacggcgtg ccggggatag agggggtcga tacccgggcg ctgaccctcc ggctgcggga
     1141 caggggcagc tgtgccggac tccttgtgag ttcctcctcc ggcagcgccc tttccgcttc
     1201 ggagatggac cgtgccctcg ccttcctgaa ggccgttccg tccatggagg gacggaacct
     1261 cctcccgtcg gtggggacgc ggtcactgaa atgccgggat gtgcccggaa accgcagatt
     1321 tgcagtagtg gactgcggga tcaagggaaa catcctccgg gaactctccc tccggggagt
     1381 gtcttcaacg gtgtttccct ggacggcaac ggtggaggaa attctccggt cttccccgga
     1441 cggggtcctt ttttccaacg gaccggggga ccc
//
LOCUS       DAOTHD010000638         1473 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_103069_length_1473_cov_3.021862, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000638 DAOTHD010000000
VERSION     DAOTHD010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103069_length_1473_cov_3.021862"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            336..707
                     /gene="queD"
                     /locus_tag="P5219_09980"
     CDS             336..707
                     /gene="queD"
                     /locus_tag="P5219_09980"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HRX27133.1"
                     /translation="MLLRKEFTFDAAHNLVEYHGKCERLHGHTYRLAVVLKGSPDREG
                     MIADFCEVSSLVKERVISRLDHAYLNDILPQPTAEYIARWVWEQVEEPLRRPNCRLWE
                     VEVWETATSCAVLTAEDMDHA"
     gene            700..>1473
                     /gene="folE2"
                     /locus_tag="P5219_09985"
     CDS             700..>1473
                     /gene="folE2"
                     /locus_tag="P5219_09985"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HRX27134.1"
                     /translation="MRDVQSERDSRRIPIDRVGVGGVSYPIVVMDQKENIQHTVASVS
                     MSVSLPHEYRGTHMSRFIEVLHEFQGKVTLPNMEHIAEDLRTVLSAECAEIVFSFPYF
                     ISRKAPVTGLTSFTRYDVSFTAVKGENVFDLVTSVTVPVQTLCPCSREISEAGAHNQR
                     AFVTIAVRMKGLVWIEELVAVAEKNASAPVYTLLKREDEKFVTEQAYNNPRFVEDVLR
                     DVAVDLEAENRITWYSIRVASSESIHNHDAFASLEREKEG"
BASE COUNT          332 a          424 c          427 g          290 t
ORIGIN      
        1 gtaaaataaa aaaacgatgc cactttatag cactctaccc gtacattagt caatagaaaa
       61 ggtaaaaagt gctgcataaa aaacaaaaac agcgttttta tgacactcca tcttcttttt
      121 aggcgggaac ttctcgaaaa aacgaacata gtcggttgtc ggtacgagag gcggatgtcc
      181 agtgttcatt cttccccggg gccggagact gcgggggcct gccggccgtt tctcttccgg
      241 ggatttggtg taaaatcaat gaggaaatga tttttgcccc tgttccgggc cggtccttac
      301 cggcgcccat ttttctcgaa aggcggggat tccccatgct gctccggaag gaattcacct
      361 tcgacgccgc ccacaacctg gtggaatacc acggaaagtg cgagcggctt catggacata
      421 cataccggct ggcggtggtg ctgaaaggct ctcccgacag ggagggaatg atagcggact
      481 tctgcgaggt ctcttccctg gtgaaggagc gggtcatctc caggctcgac catgcctacc
      541 tgaacgatat acttccccag cccacggcgg agtacatcgc ccgatgggtc tgggaacagg
      601 tggaagaacc cctccgccgg ccgaactgca ggctctggga ggtggaggtc tgggagaccg
      661 ccacgagctg cgcggtgctc accgccgagg acatggacca tgcgtgacgt tcagagcgaa
      721 cgggattccc ggcgcattcc catcgaccgg gtgggcgtcg gcggcgtgtc ataccccatc
      781 gtggtcatgg accagaaaga gaatatccag cacaccgtgg cttccgtctc catgtccgtg
      841 tcccttcccc acgagtacag gggaacccac atgagccgct ttatcgaagt gctccacgag
      901 ttccagggga aggtcaccct gcccaacatg gagcatatcg ccgaagacct caggacggtg
      961 ctctccgccg agtgcgcgga gatcgtcttc tccttcccct acttcatttc gaggaaggcc
     1021 cccgtaacgg ggctgaccag cttcaccagg tacgacgtct cctttacggc cgtcaaggga
     1081 gaaaacgttt tcgacctggt gacctccgtc accgtaccgg tacagaccct gtgcccctgt
     1141 tcccgggaga tctccgaggc cggtgcccac aaccagagag ctttcgtgac catcgctgtc
     1201 cggatgaagg ggcttgtgtg gatagaggaa ctagttgccg ttgcggagaa aaatgcctcc
     1261 gcaccggtgt atactttact gaagcgggag gacgagaagt tcgtcaccga acaggcatac
     1321 aacaaccccc gcttcgtgga ggacgttctg cgggacgtgg cggtggacct ggaggccgag
     1381 aacaggatca cctggtacag catccgggtc gccagcagcg aaagcatcca caaccacgac
     1441 gccttcgcct ccctggaaag ggagaaggaa gga
//
LOCUS       DAOTHD010000639         1472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_103184_length_1472_cov_2.949894, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000639 DAOTHD010000000
VERSION     DAOTHD010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103184_length_1472_cov_2.949894"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="P5219_09990"
     CDS             complement(<1..937)
                     /locus_tag="P5219_09990"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HRX27135.1"
                     /translation="MNTLRGRLLGIFLVLSLGGLAIIGFLAVNSSSRALVSSAEKEGL
                     ALGKGLAVETDMFFRERLNFLNLQSERNVVRTMKWETQKPGLAEAANLYDDLVDIWVV
                     SPDGSENHLTGTLAGFEGKEGLRKVLSDGKTYVSPVTVLKSAGKNVVVFGVPVESDGQ
                     RVGVLSAAFDAAGLSKFIDPVRWGTTGYAYATDRNGVILAHQNKGHIGVLDTSKAAGA
                     ITPELAEAMNKGLAGQQGLAGYSFEGSDKYVAYYPIPTIGGVLGMTSTVAEFLAPVRA
                     IRNTVIIAAAVVALLVIAVSLWMAGSIASPVRRVAE"
BASE COUNT          301 a          457 c          363 g          351 t
ORIGIN      
        1 tttcggccac ccggcggaca ggggaagcaa tgctgcccgc catccacagc gacacggcaa
       61 tgacaagcag tgccactaca gccgccgcaa tgatcaccgt attccggatc gcccggacag
      121 gggcgaggaa ctccgcaact gttgaggtca tgcccaggac accgccgatg gtgggaatgg
      181 ggtagtaggc cacgtacttg tcgcttccct cgaaggagta tccggcgagt ccctgctgcc
      241 cggccagccc cttgttcatg gcctccgcca gttcaggtgt gatggccccg gcggctttcg
      301 atgtgtccag cacgccgatg tgccccttgt tctgatgggc aaggatgacg ccgttcctgt
      361 cggtggcgta ggcgtacccc gtggttcccc agcggacggg atcgatgaat ttgctgagtc
      421 ccgccgcgtc aaaggcggcc gaaaggacac cgacccgctg tccgtccgat tcgacgggaa
      481 cgccgaaaac cacgacgttc tttccggcgc tcttcagaac cgtcacggga gaaacatagg
      541 ttttgccgtc ggaaagcact ttccgcagtc cctcttttcc ttcgaacccg gccagcgttc
      601 cggtgagatg gttttccgaa ccgtcgggag agaccaccca gatgtcgacc aggtcgtcgt
      661 agaggttcgc cgcttccgca agaccaggct tctgggtctc ccacttcatg gtcctgacga
      721 cgttccgttc agactgcaga ttgaggaaat tcagccgctc ccggaagaac atgtccgttt
      781 caaccgccag ccccttgccc agggcgagcc cttccttctc cgccgacgat accagcgccc
      841 ggctcgagct gttcaccgcc aggaaaccga tgatcgccag ccctccaaga ctcaatacaa
      901 gaaaaattcc aagcagcctt cctctcaggg tgttcatctc tatcctctcc ctttttcctc
      961 tacggatttt cgattctgca gaatgttcga ttttagtact ttatcacacc acagcccaca
     1021 tcatcaatat ggttatatat ttatcttacg ttctcttttg agaattgagg ttttgctcat
     1081 tttgtttatt ttcactccgc ttcacttttt atttttttat gcatttaccc ctctccggac
     1141 cgtaccgtgg aaaacacgag atgaccggag aacagtccgg ccgcagatat gcccgggaca
     1201 tcttttctcc tcccccacat cgccccgccg gtctttctgt accaaatttt tcgtttcttg
     1261 agacttcatt ccggtccctt gattattgtg tgatagtgat tacaataaca aatgcatagt
     1321 accctacgtt caaaaaatat cgaattcagg aggaaatccc ctatggtcac gaagaaagcc
     1381 ccccgggaag acgtccaggt tcttgattcc gtccagttca tccgcgaatc catcgatgcc
     1441 gcggtggcca ggacccgggc ggcccagcgc cg
//
LOCUS       DAOTHD010000640         1470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_103382_length_1470_cov_3.373145, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000640 DAOTHD010000000
VERSION     DAOTHD010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103382_length_1470_cov_3.373145"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..1029)
                     /gene="meaB"
                     /locus_tag="P5219_09995"
     CDS             complement(34..1029)
                     /gene="meaB"
                     /locus_tag="P5219_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRX27136.1"
                     /translation="MDILINKALAGDMRAIARIISLIENGDPIADAVMRRIYPLTGKA
                     MVIGITGSPGAGKSTLTDKLIESFRKKGKTVGIIAVDPSSPFSGGAILADRIRMQRHT
                     GDEGVYIRSMGTRGSLGGLSRGTREAVLLLDACGKDVIIIETVGVGQSEVDIIKIADT
                     VCVVLVPGMGDDIQIMKAGIMEIANVFAINKADKPGVDRVAAEVRLMLELVRDKSWTP
                     PVHLTVAENGTGVEEFAESIVKHHEFIKNSPEGARFEFRRLSSEVEDILLKNLTRKAE
                     KVWRSQKSGKLMEDLAARKTNPYTVASDILRKLFPQADYDEGIKEANTSHVTPDN"
     gene            complement(1035..1451)
                     /locus_tag="P5219_10000"
     CDS             complement(1035..1451)
                     /locus_tag="P5219_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HRX27137.1"
                     /translation="MDERKIRVVVAKPGLDGHDRGAKVVARALRDAGMEVVYTGLRQT
                     PEQIVETVLQEDADAVGISILSGAHTFYFTKIIGLLKEKDAGDVIVFGGGVIPDKDIP
                     GLLEAGAGAVFGPGTPTSVCVEWLEKAVADKRAKEQ"
BASE COUNT          269 a          455 c          368 g          378 t
ORIGIN      
        1 tcgtgcttct tcagaagctc ctcgcacaac tcctcaattg tccggtgtga catgtgatgt
       61 gtttgcctcc tttatcccct cgtcgtagtc cgcctgcggg aacagcttcc tgagtatgtc
      121 cgaggcaacg gtgtaggggt ttgtttttcg tgccgcaagg tcttccatga gcttcccgct
      181 cttttgcgac cgccagacct tttccgcctt ccgcgttaaa ttcttcagaa gaatatcctc
      241 cacctcggag gataggcgcc tgaattcgaa acgggcccct tccgggctgt ttttgatgaa
      301 ttcatggtgt ttgacgatgc tctccgcgaa ctcttccacc ccggtgccgt tctccgcaac
      361 cgtaaggtga acggggggcg tccatgactt gtccctgaca agttcaagca taagcctcac
      421 ttcggcggcg accctgtcta cgccgggctt gtcggccttg ttgatggcaa agacattggc
      481 gatttccata atgcccgcct tcattatctg gatgtcgtct cccatgcccg ggaccaggac
      541 aacgcacacc gtatcggcta ttttgatgat atccacttcc gactggccga cgccgacggt
      601 ttcgatgatg atgacatcct tgccgcaggc gtcaagaagc agcactgctt ccctggtccc
      661 tctgctcagg ccgccgaggg accccctggt tcccatgctc cgtatataga cgccttcgtc
      721 tcccgtgtgg cgctgcattc tgatgcgatc tgcaagaatg gctccgcccg agaaggggct
      781 cgagggatcg acggcgatga tacccacggt cttccccttt ttgcgaaaag attcgatcag
      841 cttgtcggtc agcgtgcttt tccctgcgcc tggacttccg gtaatgccga taaccatggc
      901 ttttccggta agggggtaaa tacggcgcat cacagcgtcg gcaatagggt caccgttttc
      961 tatcagactg atgatcctgg ctatggcgcg catatcgccc gccagggctt tattgataag
     1021 aatatccaca gcgtctactg ttctttggcc cttttgtccg ctacagcttt ttcaagccat
     1081 tcaacgcaaa cagacgtggg ggttcccggt ccgaataccg ccccggctcc cgcctcgaga
     1141 agtcccggga tatccttgtc cggaatgact ccgccgccga agacgataac gtctcccgca
     1201 tccttttcct tgaggagacc gatgatcttc gtaaaataaa acgtgtgggc gccggaaaga
     1261 atgcttattc ccacagcatc ggcatcctcc tgaaggactg tttccacgat ctgctccggg
     1321 gtctgcctga gtcccgtata gaccacttcc atgccggcgt cccgcagagc tcttgccacg
     1381 actttcgctc ccctgtcgtg accgtcgagt cccggttttg ccacaaccac cctgattttt
     1441 ctctcgtcca cggaatatcc ctccagtgtg
//
LOCUS       DAOTHD010000641         1469 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_103464_length_1469_cov_3.770863, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000641 DAOTHD010000000
VERSION     DAOTHD010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103464_length_1469_cov_3.770863"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="P5219_10005"
     CDS             complement(<1..486)
                     /locus_tag="P5219_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27138.1"
                     /translation="MMKKSVPAAAVVFLVLLLPGILMCQPLEQVIDQKIILPGGDKTP
                     YSVRELFMDIIVGSVSYQLNDGGLSSFALSSPGLAKGAALFHFEQQRKIAYKNYERSL
                     EYGSEADRKYYEGVIQRLEAAKVWISTGDGAPLRAALQNKQKGAVPQKKSPPSTVFKG
                     SI"
     gene            694..990
                     /locus_tag="P5219_10010"
     CDS             694..990
                     /locus_tag="P5219_10010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HRX27139.1"
                     /translation="MIIVSASFSAKPGMRDAIAEISRKAVELTRKEKGNISYTLFKSS
                     DDDVSMMYFEEWESMDDLRAHLKTDHIREAREARKDMLDGPVRVRVFESKEVEL"
     gene            1012..>1469
                     /locus_tag="P5219_10015"
     CDS             1012..>1469
                     /locus_tag="P5219_10015"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRX27140.1"
                     /translation="METGMYDAIVVGGGLAGLSAAIEAANRGLGVLLICKSSAGRSGN
                     TLVSGAALSVMNAGAGDSNSLLEEDILSSGCGMSDPDICRVFSKESASALCELKESGV
                     AFKEPGGIPMVKQPPGHSVPRMFPSEYRKFPYKNRGLALTLPLVKRAEKLG"
BASE COUNT          327 a          378 c          423 g          341 t
ORIGIN      
        1 gattgaaccc ttgaaaacag tggagggcgg agattttttt tgaggaaccg cccctttctg
       61 tttattctgc agggcagccc tcaaaggagc gccgtctccc gtggaaatcc atacctttgc
      121 ggcctcgaga cgctgaatga ctccttcgta gtacttccgg tcggcctcgc ttccatattc
      181 cagagaccgt tcataatttt tgtacgctat ttttctctgc tgttcgaagt gaaagagggc
      241 cgcgcctttt gcaaggccgg gagacgacag ggcgaagctc gacagtccgc cgtcgttcag
      301 ctggtagctt acggaaccca cgattatgtc catgaagagt tcccggacgc tgtacggagt
      361 cttgtcaccg cccggaagga tgatcttctg atcgataacc tgttccaggg gctggcacat
      421 gagaattcca ggaagaagca ggaccaggaa aacaactgcc gctgcaggaa cacttttttt
      481 catcatctcc cacctccatt tttcggagtc tttgtccacc ttaccattaa acgcaaaaaa
      541 ttaaaacctt ttcagagtgt ggtaagggtt tgagtcttcg ctgtatttac cctctgtccg
      601 gtaggggtat aataagtcag cagggcagaa tgcactttcg gtgcagaagc catgcctgtc
      661 cgctacatta tccctttcgg aggaggaaga atcatgatta tcgtatccgc atccttttct
      721 gcgaaacctg gaatgaggga cgccatcgct gagatttccc gaaaggccgt cgaactcacg
      781 agaaaggaaa aggggaacat cagctacacc ctcttcaaaa gcagcgatga cgacgtctcc
      841 atgatgtatt tcgaggagtg ggagagcatg gacgacctga gggcccacct gaagacggac
      901 catatccgcg aggccaggga ggcccggaag gatatgctgg acggcccggt ccgggtacgg
      961 gtgttcgagt cgaaggaagt ggaactttag acaggagctg gtttattttt tatggaaacc
     1021 gggatgtacg atgcgattgt cgtcggcgga ggccttgcag gcctgtctgc tgccattgaa
     1081 gcggcgaata gagggctcgg cgttctcctg atctgcaaga gcagtgcggg acggagcggc
     1141 aataccctcg tctccggcgc cgccctttcg gtgatgaacg ccggggccgg ggattccaac
     1201 tccctgctgg aggaggatat tctctcgtcg gggtgcggaa tgagcgatcc ggatatttgc
     1261 cgcgtttttt caaaagaatc ggcttcggca ttgtgtgagc tgaaggaatc cggtgtcgcg
     1321 ttcaaggagc cgggggggat tcccatggtg aagcagcctc ccggacacag cgttccccgc
     1381 atgttcccgt cggaataccg gaagtttccc tacaagaacc gcggcctcgc cctgacgctt
     1441 cctcttgtga aacgggcgga gaaactcgg
//
LOCUS       DAOTHD010000642         1469 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_103487_length_1469_cov_2.901697, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000642 DAOTHD010000000
VERSION     DAOTHD010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103487_length_1469_cov_2.901697"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..788)
                     /locus_tag="P5219_10020"
     CDS             complement(132..788)
                     /locus_tag="P5219_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX27141.1"
                     /translation="MPLHIGAIDDDRSILYTLEAMASTEGWRMLTTSEPEECLRWVKL
                     DEVDILLVDYHMPVMNGLHIIRKARELSAKLVLIALTVEDGEELASRLTLAGADDFIS
                     KPIRLADFTARIRLHEKLCAHREQLNWEERKKGVSKDTMRKVLEKLKEFPSPRDCDDV
                     AAACGLAYVTAHRYLEYLADRGLIVRSAGVQDGRPGRPKTYYAISGPSALAEEKKENG
                     "
     gene            complement(788..>1469)
                     /locus_tag="P5219_10025"
     CDS             complement(788..>1469)
                     /locus_tag="P5219_10025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRX27142.1"
                     /translation="DRENKMARLREQSIASRSLVEMQQLVHDLKRPLTTILGLGDVIV
                     SGRGGGNTAKYGTVICEAARTMEEMVSEILHEDFRRPVLLGDLVEYVFTQISPFSWRE
                     TVSLEADRPVLMETVEVNVVRFSRALVNLLDNAHKANAQSGGGKITLSAELDERSVII
                     SVADEGPGFPPDWKAGSGWNSTGLGLQYVTMVVCNHGGTFRTENLPGGGARTELTLGR
                     AGKGKDEQ"
BASE COUNT          272 a          446 c          414 g          337 t
ORIGIN      
        1 cgaaaggagc ggcaagggcg tcttctggag ttcccgctgg aggccggttg ccccgggaac
       61 ggggaatcac gacgtcccgg ggaggcgtcc agggtacggt ctttcttccc aggggtatat
      121 gcagcatccg ttcagccatt ctctttcttc tcctctgcaa gggccgaagg gcccgatatg
      181 gcgtagtagg tcttcggacg tccggggcgt ccgtcctgaa ccccggcgct cctcacgatg
      241 agtccccggt cggcgagata ctccagatac ctgtgggcgg tgacataggc aaggccgcag
      301 gccgccgcaa cgtcatcaca atctcttggg gaaggaaact ccttgagttt ttccagtacc
      361 ttccgcatgg tgtccttgct cacccctttt tttctttcct cccagttgag ctgctccctg
      421 tgggcacaga gtttctcgtg aagcctgatg cgggctgtga agtccgcgag ccgtatgggc
      481 ttggagatga aatcatcggc tcccgccagg gtaagtctgg aagcaagctc ttccccgtct
      541 tccacggtaa gggcgataag aacaagtttt gccgaaagct cccgggcttt gcgaatgatg
      601 tggagcccgt tcatgacagg catgtggtag tccacgagaa gaatgtcaac ctcgtcgagc
      661 ttcacccacc ggaggcactc ctccggttcg ctcgtggtga gcattctcca cccttccgtg
      721 gaggccatgg cttcgagagt gtagagaatg gacctgtcat cgtcaattgc cccgatatga
      781 agcggcatca ctgctcatcc tttccttttc cagccctgcc aagggtcagc tccgtccgtg
      841 caccgccccc ggggaggttt tcagtccgga acgttcctcc gtgattgcat accaccattg
      901 taacgtattg gaggccaaga ccggtagagt tccaccccga tcctgctttc cagtccggcg
      961 ggaaaccggg gccttcgtcg gcaactgaaa tgatgacgga acgttcatca agttctgcgg
     1021 acagggtgat ttttcctccc cccgactggg cgttcgcctt gtgggcgttg tcaagaaggt
     1081 tcaccagggc cctggaaaag cgcaccacat tcacttccac cgtctccatc aggacaggac
     1141 ggtctgcttc aagcgaaacc gtctctctcc acgaaaaggg gctgatctgg gtaaagacat
     1201 attccacgag atcccccaga agcaccggtc tcctgaaatc ttcgtggagg atttcagaga
     1261 ccatttcttc cattgtccgt gccgcctcgc agatgacggt tccgtacttc gccgtgttcc
     1321 ctcctccccg cccggacaca atgacgtccc cgaggccgag aatggtggtg aggggccgtt
     1381 tcaggtcgtg gacaagctgc tgcatttcca caaggctccg gctggcgatg ctctgctccc
     1441 ggaggcgggc cattttgttc tccctgtcc
//
LOCUS       DAOTHD010000643         1467 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_103720_length_1467_cov_2.539660, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000643 DAOTHD010000000
VERSION     DAOTHD010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103720_length_1467_cov_2.539660"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="P5219_10030"
     CDS             complement(<1..275)
                     /locus_tag="P5219_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HRX27143.1"
                     /translation="MIRLSTVSALVFLSILHFFSFSGEGAPLPPEEIAGKYRNASPVQ
                     WGEHVSGTASRIAAKGKEIALTFDACGGKKGSAVDRDLLDFLRQERIP"
     gene            337..819
                     /locus_tag="P5219_10035"
     CDS             337..819
                     /locus_tag="P5219_10035"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRX27144.1"
                     /translation="MGHPVRRKDREITEGAEMYEILDRAFTGHLALCSGGEPYVVPLC
                     FGVMNGAVYFHCAREGRKLDILARNPRGCFQAQCDEDIVRSEERPCGWGMFYKSVMMS
                     GPVTVVEDEQEKAAALLAIMKKYAGEDFSHEFSPAECASVTVLRLDPEERSGKARRPA
                     "
     gene            832..>1467
                     /locus_tag="P5219_10040"
     CDS             832..>1467
                     /locus_tag="P5219_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27145.1"
                     /translation="MFRIRRIFDDLSPANETALVRIGEILKEQFPGARPGEAESIPEK
                     LRNQLKYGFQTILFAAEKGAGKVHGFALALYDPLLKFTFLDYIAVPGAEAGGGLGGIL
                     YERVRKEALDLGCLGLFFECLPDDPELSRDEETRKQNADRLRFYERYGAVPVTGTKYE
                     TPLEPGGDNPPYLVFDGLARGEPLRREKARLIVAAILGKKYGDLCPPEYIRM"
BASE COUNT          294 a          423 c          481 g          269 t
ORIGIN      
        1 ggaatccgct cctgtctcag aaaatccagg agatcccggt ccacggccga tcccttcttt
       61 cccccgcagg cgtcgaaggt cagggcaatt tcctttccct tggcggcgat tcttgaggct
      121 gttccggaaa catgctctcc ccactggacg ggggatgcgt tccggtactt tccggcgatc
      181 tcctccggcg gaaggggcgc tccctcgcct gaaaagctga aaaagtgtag aattgacaga
      241 aacacgaggg cggagacggt ggacaaacgg atcatctctc aggtacctcc atgaggttca
      301 gtatatcatt tcattataac ggaggaggat ttgactatgg gccatccggt gcgcagaaaa
      361 gacagggaga tcactgaggg cgcggaaatg tacgaaattc tggacagggc gttcaccggc
      421 catctcgccc tctgcagcgg cggggagccc tacgtggtgc ccctctgttt cggggtgatg
      481 aacggcgccg tttatttcca ctgcgcccgg gaggggcgga agctcgatat cctcgcccgg
      541 aacccccggg gatgcttcca ggcccagtgc gacgaggata ttgtgcggtc tgaggagcgg
      601 ccctgcgggt gggggatgtt ctacaaaagc gtcatgatgt cgggaccggt aacggtggtg
      661 gaggacgaac aggagaaggc cgccgccctc ctggcgatca tgaaaaaata cgcgggggag
      721 gacttctccc acgaattctc cccggccgaa tgcgcctccg tgacggtgct ccgcctcgac
      781 cccgaggagc gttccggcaa ggccaggcgt ccggcatagg gggcggtgcg ggtgttccgt
      841 atccggagga tcttcgacga cctttccccg gcaaacgaaa cggcgcttgt ccgcataggg
      901 gaaattctga aagagcagtt tccgggtgcc cgccccggcg aggccgaatc catcccggag
      961 aagctgcgga accagctcaa gtatggcttc cagaccattc tcttcgcagc ggagaagggg
     1021 gccgggaagg tccatggttt tgccctggcc ctgtatgacc cgctgctgaa gttcactttt
     1081 ctggactata tcgccgttcc gggggccgaa gcgggcggag gtctgggagg catactctat
     1141 gagagggtgc gcaaggaagc cctggatctc ggatgcctgg ggcttttctt cgaatgcctt
     1201 cccgacgacc cggaactcag ccgggatgag gaaacccgga agcagaacgc cgaccgcctg
     1261 cgattctacg agcggtacgg cgccgtgccc gtgacgggga cgaaatacga gacgcccctg
     1321 gagccgggag gggacaaccc cccgtacctg gtcttcgacg gcctggcaag aggggagccc
     1381 ctgaggaggg aaaaggcccg gctcatcgtg gccgccatcc tggggaagaa atacggagat
     1441 ctctgcccgc cggagtatat ccgcatg
//
LOCUS       DAOTHD010000644         1463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_104160_length_1463_cov_2.433239, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000644 DAOTHD010000000
VERSION     DAOTHD010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104160_length_1463_cov_2.433239"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="P5219_10045"
     CDS             <1..465
                     /locus_tag="P5219_10045"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="catalyzes the interconversion of 3-phosphoglycerate
                     and 2-phosphoglycerate; this enzyme does not require the
                     cofactor 2,3-bisphosphoglycerate as a phosphate donor;
                     BPG-independent PGAM; aPGAM; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HRX27146.1"
                     /translation="VATYPMYRGLAKLVGMEVVDAGETLDHLFDTVGRLWNDYDYFYV
                     HVKYTDSRGEDGDFDGKRKVVETVDELLPKLLALSPGVLVVTGDHSTPSVMSAHSWHP
                     VPFLLSGSFVRSGDSKAFSERECRLGSGGRMEARKLLGLMLANAGRLEKYGA"
     gene            477..>1463
                     /locus_tag="P5219_10050"
     CDS             477..>1463
                     /locus_tag="P5219_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRX27147.1"
                     /translation="MTIRGDGVEEQRLSKTGEIRRMSKDERFKVLGVVSRTSVRKDKN
                     GKNYWDIALMDEEGVIEGKIWGNSRWWDKSGESQTEVGDPAESPIFSELVGKTLGLAG
                     QVTEFKGQPQYNFSGVYYMNQEKFPPHQFVQRSPIGQDTLEKEFLDLLESCGGQVEAF
                     LRYVFFERKLWDRFKDWPAAVAHHHAYVGGLLEHTVAVARTARSLAGTYSESGYEINV
                     PVAVAGALLHDLGKMDSYRLVPAPEMTVVGTVIDHIVLGYSTFSSLAGDFGLEERLFL
                     AIGHILVSHHGCREFGSPVLPATPEAMVVSAADELDFRLFCWKNSVEKMEDGK"
BASE COUNT          331 a          380 c          451 g          301 t
ORIGIN      
        1 gtggcgacct accccatgta ccgggggctg gcgaagctcg tcgggatgga ggtcgttgac
       61 gccggcgaga ccctggacca tctcttcgac accgtcggcc gcctctggaa cgactatgat
      121 tacttctacg tccacgtgaa gtacacggac agcaggggag aggacggaga cttcgacggc
      181 aagcggaaag tggtggagac ggtggacgaa cttctgccga agctccttgc cctgtctccc
      241 ggcgtgctgg tggtgaccgg tgaccacagc acccccagcg tcatgagcgc ccactcctgg
      301 catcccgtac ctttccttct ttcgggctct tttgtgcgct caggtgattc aaaggcattc
      361 agcgagcggg aatgccggct cggatcgggg ggaagaatgg aggcgaggaa actgctcgga
      421 ctgatgctcg ccaacgccgg acggcttgaa aaatacggcg cgtaaggcca ttatcaatga
      481 ctattcgggg tgacggtgtg gaagagcagc ggctgagcaa aactggagaa atccggcgga
      541 tgtccaagga tgaacggttc aaggtccttg gagttgtttc gagaacctct gtgaggaagg
      601 acaaaaacgg aaagaattat tgggacattg cccttatgga tgaagagggc gtcattgaag
      661 gcaaaatctg gggaaattca aggtggtggg acaagagcgg cgagagccag actgaagtcg
      721 gcgatcctgc cgaatcaccc atctttagtg agcttgtggg caaaacactg ggactggcgg
      781 ggcaggtcac ggaattcaaa ggacagcccc agtataactt cagcggggtc tattacatga
      841 accaggagaa attcccccca caccagtttg tccagcgttc tcccatcggc caggataccc
      901 tggagaagga attcctcgat cttctggaga gttgcggcgg acaggtggag gcatttctcc
      961 gctatgtatt cttcgagaga aaactctggg accggttcaa agactggccg gcagcggtgg
     1021 cccatcatca cgcctacgta ggggggcttc tggagcatac cgttgccgtg gcgagaacgg
     1081 ccagatctct tgcaggaaca tactctgaat cggggtatga aatcaatgtc cccgtcgcag
     1141 tagctggtgc cctgctgcac gatctcggaa aaatggattc ctacaggctc gttccggctc
     1201 ctgaaatgac ggttgtcggt actgtcatag accatatcgt cctgggatac agcacattct
     1261 cctccctggc aggggatttc ggccttgagg aacggctttt tctcgccatc ggccatatac
     1321 tggtaagcca ccacggctgc cgggaattcg gatcccccgt tctgcccgcc actcccgagg
     1381 ccatggtagt gagcgcggcc gacgaactcg acttcaggct tttctgctgg aagaattctg
     1441 tggagaagat ggaggacgga aag
//
LOCUS       DAOTHD010000645         1461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_104391_length_1461_cov_1.741110, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000645 DAOTHD010000000
VERSION     DAOTHD010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104391_length_1461_cov_1.741110"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="P5219_10055"
     CDS             <1..793
                     /locus_tag="P5219_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YidE/YbjL duplication"
                     /protein_id="HRX27148.1"
                     /translation="AGTYTGALTSSPGLGAALEATGGNPDVTIGYSVAYPFGVMAVVL
                     FVQLIPAIFGIDVAKEKEKLAAEKKTASPEEVEKRSESPAFSLLSFVFCILGGSLLGK
                     VSVPLGGLGSIALGTTGGALLFALGAGSFERIGPFPMRMDKKVLSAIRSISLGFFLAI
                     VGLNAGGGVVKAFLEHGVLLIAVGIGAALAAEMTGFFLGYYLWKINWIILAGAICGAM
                     TSTPGLGAAIDATGTDEVSAGYGGAYPVAIVCMVLFTTMLHSFFH"
     gene            complement(871..>1461)
                     /locus_tag="P5219_10060"
     CDS             complement(871..>1461)
                     /locus_tag="P5219_10060"
                     /inference="COORDINATES: protein motif:HMM:NF023990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3754 domain-containing protein"
                     /protein_id="HRX27149.1"
                     /translation="IGGLFSVVNKVIPALVVIGTVITALVLGHTINWSDFQDKLFPIL
                     AAFSVVGMIAFKVFAKYKNTKEKHQARLMKTLYFHNLDNNGGVFDFLVNEAEEEECKE
                     IILAYYFLLAERNASGLPFTREELDDRIEEWMQEKFGVAMDFEVRDAVRKLEEKGILE
                     QKDGRYSVPSIRKTLETLDGLWDNFFPYNNEATAES"
BASE COUNT          241 a          455 c          446 g          319 t
ORIGIN      
        1 cgccggaacc tacacgggag ccctgaccag ctctcccggc ctgggggctg ccctggaggc
       61 cactgggggg aatcccgacg ttaccattgg gtattccgtg gcctatccct tcggcgtcat
      121 ggcggtggtg ctctttgtcc agctcattcc ggccatcttc gggatcgacg tggcgaagga
      181 gaaggaaaag ctcgccgcgg agaaaaaaac cgcctcccct gaggaagtgg agaagcggtc
      241 cgaatccccg gccttttccc tgctctcctt cgtcttctgc atcctcgggg gaagcctgct
      301 cggcaaggtg tccgttcctc tcggcggcct tgggtccatc gccctcggaa ccacgggagg
      361 ggcgctcctc tttgccctcg gggcggggag cttcgagcgc atcggtccct tccccatgag
      421 aatggacaag aaggtcctct ccgccatccg gagcatctcc ctcgggttct ttctcgccat
      481 cgtggggctg aacgccgggg gcggagtggt gaaggccttc ctggagcacg gggtcctgct
      541 cattgccgtg ggcatcggag cggctctcgc ggcggagatg acgggttttt tcctggggta
      601 ttacctctgg aagatcaact ggatcatcct cgccggggcc atctgcgggg ccatgaccag
      661 cacgcccggc ctcggggcgg ccatcgacgc cacgggcacc gacgaggtga gcgccggcta
      721 cggcggagcc taccccgtgg ccatcgtctg catggtgctc tttaccacca tgctccattc
      781 cttcttccac tgagcttttt ttgcgcaaaa aggcagcccc ttccccggac gctccggaga
      841 aggggctgct cttctttgta agccgctgcc tcaggattcc gccgttgcct cgttgttgta
      901 ggggaagaag ttgtcccaca ggccgtccag ggtttccagg gtcttccgga tggacggcac
      961 ggaataccgc ccgtccttct gctcgaggat tcccttttct tccagtttcc ggacggcgtc
     1021 cctgacctcg aagtccatgg cgacgccgaa cttttcctgc atccattcct cgatccggtc
     1081 gtccagttcc tcccgggtga agggaaggcc cgaagcattc cgctcggcga ggaggaagta
     1141 gtaggccagg atgatttcct tgcattcctc ttcctccgcc tcgttcacaa gaaagtcgaa
     1201 caccccgccg ttattgtcca gattgtggaa atagagggtt ttcatgagac gggcctggtg
     1261 cttttccttg gtgttcttgt acttggcgaa gaccttgaag gcgatcatgc ccaccacgga
     1321 gaaggcggcc aggatgggga agagcttgtc ctggaaatcg ctccagttga tcgtatgccc
     1381 gaggaccagg gcggtgatga cggtgccgat gaccaccagg gccggaatca ccttgttcac
     1441 cacgctgaag agtccgccga t
//
LOCUS       DAOTHD010000646         1460 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_104442_length_1460_cov_3.348043, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000646 DAOTHD010000000
VERSION     DAOTHD010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104442_length_1460_cov_3.348043"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1460)
                     /locus_tag="P5219_10065"
     CDS             complement(<1..>1460)
                     /locus_tag="P5219_10065"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRX27150.1"
                     /translation="ELVVNPTEEQMLVSELDLLVAGHLDGVTMVECGSREVSEEILVD
                     ALDLAQSEIRKIVGLFNEMRASVGREKLVLPVPPTFPDIDRWIADNLGAEIREAVQTH
                     EKKPRGDKLSAARNKAQEHFAEEYPDSGDYIAAFIDEMVKKTMRSLIVDERVRVDGRA
                     MDELRPITCETGILPRVHGSALFTRGETQALVVTTLGMMGVDDQILDGLKQDEPAKRF
                     ILHYNFPPYSVGEVRPMRGPGRREIGHGALAERALRSMIPEEADFPYVMRVVSDILES
                     NGSSSQASICGGSLALMNAGVPMKKHVAGIAMGLIKEGDKVAVLTDIQGLEDHFGDMD
                     FKVAGTRDGVTALQMDNKAGGITRGILEQALGQAKAARMAILEKMEAAIPAPAQLSPN
                     APRIFMTTIDPEKIRDVIGPGGKVIRGITQKTGVKINVEDNGEIYIGGSSQDKVDEAL
                     AIIRGLTKDLEAGEVYYGTVTRLMAFGVFIECLPGK"
BASE COUNT          255 a          442 c          442 g          321 t
ORIGIN      
        1 tccttgccgg ggaggcattc gatgaacacg ccgaaggcca tgagcctggt gaccgtgccg
       61 taatacactt cgccggcctc gaggtccttc gtgaggccgc ggataattgc aagggcctcg
      121 tcaaccttgt cctgggaaga tccgccgatg tagatttcac cgttgtcttc cacgttgatc
      181 ttcacaccgg tcttctgggt gattccccgg atgacctttc cgccggggcc gataacgtcc
      241 cggatctttt cgggatcaat ggtggtcatg aagatcctgg gggcattggg agaaagctgt
      301 gccggcgcgg gaatggcggc ctccatcttc tcgaggatgg ccatccgtgc cgcctttgcc
      361 tgcccgaggg cctgctccag gatacccctg gtgatgcccc cggccttgtt gtccatctgc
      421 agggccgtga ctccgtccct ggttccggcc accttgaagt ccatgtcgcc gaagtggtcc
      481 tcgaggccct ggatatccgt caggacggca accttgtccc cttccttgat gaggcccatg
      541 gcgatgccgg caacgtgttt cttcatggga acgccggcgt tcataagggc aaggcttccg
      601 ccgcagatgg aagcctggga gcttgaaccg ttggattcga ggatgtcgga gacaacccgc
      661 atgacgtaag ggaaatcggc ctcttcgggg atcatggagc ggagcgccct ttcggcaagg
      721 gctccgtgtc cgatttctct tctgcccggc cccctcatgg gacggacttc gcctacggaa
      781 tagggtggga agttgtaatg aagaatgaac ctcttggcgg gctcgtcctg cttcagcccg
      841 tccagtatct ggtcatccac gcccatcatc ccgagagtgg tcaccacgag ggcctgggtt
      901 tcgccccggg taaagagagc agaaccgtga accctgggga gaatccctgt ctcgcaggtg
      961 atggggcgaa gctcgtccat ggccctgccg tcaacccgga cccgttcgtc aacgatgaga
     1021 gagcgcatgg tcttcttgac catctcgtcg atgaaggcgg cgatatagtc tccgctgtca
     1081 ggatactctt ccgcaaaatg ctcctgggcc ttgtttcttg ccgcggacag cttgtcgccc
     1141 cggggtttct tttcatgggt ctggacagcc tcccggatct cggcgccgag attgtcggca
     1201 atccaccggt cgatgtcagg gaaggtcgga ggcaccggga gaacgagttt ttccctgccg
     1261 acggatgccc gcatctcgtt gaagagaccg acgatcttcc tgatctcgct ctgggcgagg
     1321 tcaagagcat ccacgaggat ctcctcggaa acttccctgg atccgcactc caccatggtg
     1381 acgccgtcga ggtgtcccgc cacgaggaga tcgagctccg agacgagcat ctgctcctcc
     1441 gtggggttca ccacgagttc
//
LOCUS       DAOTHD010000647         1459 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_104578_length_1459_cov_2.542023, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000647 DAOTHD010000000
VERSION     DAOTHD010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104578_length_1459_cov_2.542023"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..>1459
                     /gene="mrdA"
                     /locus_tag="P5219_10070"
     CDS             99..>1459
                     /gene="mrdA"
                     /locus_tag="P5219_10070"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRX27151.1"
                     /translation="MPDFRPVLNLRLKLWRAAVLLSMGILVGGLYFFQVVHADTYIRL
                     ASNNRLRLIRFPPVRGEIYDRNGALLAVNVTTFDIMGYPMDIEKNDMPERLAELLSAH
                     GIPYTAEDLSRSIKRQSWAPYRVVRLVGNITLAQMADLVADPEFPRELFPLPVWRRTY
                     PAGSLASNITGYVGEISENELRQFSEGNYAAGDQIGKNGIERQYEDVLRGTPGEESVE
                     VDARGRKIRTIDTRPAGRGEDLHLTLDLGAQRLAADLMKGYRGAIVVMDAHTGEVLVL
                     YSSPSYDNNPLAWGVSLREWNALVKDPEKPMLDRSISGQYPPASTFKALMGLAALEEK
                     ETTTALTAHCSGAFTVGNRTFRCWKRSGHGTVNLLRALQESCDVYFYQVGLKLGISRI
                     LKWCSLFGLGSPTGIDLPGESAGVTAGPDWKRARFKESWYQGDTVNYSIGQGFLLTTP
                     LQIA"
BASE COUNT          289 a          442 c          441 g          287 t
ORIGIN      
        1 gctggggatc ttcaccacct attttctcca gcagagctac ctgcttccac tggtgctgtt
       61 cgcctctctc atatacggat ggaggctgaa gaactccgat gcctgacttc cggccggttc
      121 tcaatctccg cctgaagctg tggcgggcgg ccgtcctgct ttccatgggg atcctcgtcg
      181 ggggcctgta ttttttccag gtggttcatg ccgataccta catccggctc gcgtcgaaca
      241 accgcctccg gctcatccgc tttccgcccg tcaggggaga gatttatgac cggaacggcg
      301 ccctgcttgc ggtgaacgtg accaccttcg acatcatggg ctaccccatg gatatcgaga
      361 agaacgatat gccggaacgg cttgcggagc ttttgtccgc ccacggcatc ccctatacgg
      421 cggaggatct ttccaggtcc atcaaacggc agtcatgggc tccgtacagg gtcgtaaggc
      481 ttgtggggaa catcaccctt gcccagatgg cggacctggt ggccgatccc gaatttccca
      541 gggagctctt cccccttccg gtctggagga ggacctaccc ggcgggaagc ctcgcatcga
      601 acatcacggg ctacgtcgga gaaataagcg agaatgaact ccggcagttc agcgaaggaa
      661 actacgccgc cggggaccag ataggaaaga acggcatcga gcggcagtac gaagatgttc
      721 tccggggaac ccccggggag gaatcggtgg aagtggacgc aaggggccgg aaaatcagga
      781 ccatcgatac caggccggcc gggcggggag aggacctgca cctcaccctc gacctggggg
      841 cccagcgcct cgccgcggat ctcatgaagg ggtacagggg ggccatcgtg gttatggacg
      901 ctcacaccgg cgaggtgctt gtcctgtatt cctccccttc ctacgacaac aatcccctgg
      961 cgtggggcgt ctctctcagg gaatggaacg ccctggtgaa ggatcccgaa aaacccatgc
     1021 tcgacaggag catctcgggc cagtatcctc cggcttccac cttcaaggcc ctcatgggac
     1081 tcgccgccct ggaagaaaaa gagacgacca cggcccttac tgcacactgc tcgggggcct
     1141 tcaccgtggg gaaccggaca ttccgctgct ggaaacggtc gggccatgga acggtgaatc
     1201 ttctgcgggc cctccaggaa tcctgtgatg tatacttcta ccaggtgggg ctcaagctcg
     1261 gaatttcaag gattttgaaa tggtgttccc tcttcggtct cggttccccc acgggaattg
     1321 acctccccgg cgaatcggcg ggggtgacgg cgggacctga ctggaagcgg gccaggttca
     1381 aggaatcgtg gtaccagggc gacacggtga attactctat tggacagggg ttcctgctca
     1441 ccacgcctct ccagatcgc
//
LOCUS       DAOTHD010000648         1459 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_104581_length_1459_cov_2.514245, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000648 DAOTHD010000000
VERSION     DAOTHD010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104581_length_1459_cov_2.514245"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="P5219_10075"
     CDS             complement(<1..857)
                     /locus_tag="P5219_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRX27152.1"
                     /translation="MIVVSRLRVVIASLLLVAVMAGGAFASGELNAYTIMPEKYASQV
                     FEAFTAETGIKVNFMRFSSGEALARVVAEKNNPQVDMILGGPADTYEAGIKEGVFEAY
                     RPAGADGIPDKFRSKENFWTGIGIIPLVFLTNTKFLADKGLEAPASWNDLLDPAYKNG
                     LQMADARTSGTATERIYSLVKILGEDEAFAYQKKLHQNIQMYTKSGAGGAMPVATGQA
                     ASGIFYIVDALDIQQQGHPVVISYPKEGVSFGIEATGVLKGARNLENAKKFMDWASSK
                     TFAQLLVDR"
     gene            complement(1068..>1459)
                     /locus_tag="P5219_10080"
     CDS             complement(1068..>1459)
                     /locus_tag="P5219_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27153.1"
                     /translation="HEVAAYAVDPAFQNGTCEYFPLVVNPDRWNDLPQEYKDIVIIAR
                     DRIRYLSAMVYVHEIKAREKWKEMPNVTVVRWSPEDEAKARSVGHKLVVEECGKSAEG
                     KEFLEIYRTVLWDLGYKDEAKELGFSK"
BASE COUNT          266 a          429 c          424 g          340 t
ORIGIN      
        1 ttcctgtcca ccagcagctg ggcgaaggtt ttcgaggaag cccagtccat aaactttttg
       61 gcgttctcca gatttctggc gcccttcagg acaccggtag cctcgatgcc gaaggaaact
      121 ccctccttcg ggtagctgat cacgacaggg tgtccctgct gctggatgtc cagggcgtcc
      181 acgatgtaga aaatgccgga ggccgcctgt cccgtcgcca cgggcatggc gccgccggcg
      241 ccgctctttg tgtacatctg gatgttctgg tggagtttct tctggtaggc gaaggcctcg
      301 tcctcgccca gaatcttcac aagggagtag attcgctccg tggccgtgcc ggaggtcctg
      361 gcgtcggcca tctggagacc gttcttatag gcggggtcga ggaggtcgtt ccacgacgcg
      421 ggagcttcga gccccttgtc cgccaggaac ttcgtgttgg tgaggaagac cagcgggatg
      481 atcccgatgc cggtccagaa gttctctttc gagcggaact tgtccgggat gccgtcagcc
      541 cctgcgggcc tgtaggcctc gaagacccct tcctttatgc ccgcctcgta ggtgtcggcg
      601 gggccgccga ggatcatgtc cacctgggga ttgttcttct ccgccactac cctggcgagc
      661 gcctcgccgg aggaaaaccg catgaaattc accttgattc ccgtttcggc ggtgaaggcc
      721 tcaaaaacct gggaggcgta cttctccggc atgatggtgt aggcgttcag ttctcccgaa
      781 gcgaacgctc ctccggccat gacggcgaca aggagcagtg aagcgatgac gactctgagt
      841 ctggacacaa cgatcatctc cttctttttt tgcatgcaat gtaaacgctt tgttcctgca
      901 cctttattat ggccccggtg ctcctgtttc gcaacatgga cctgaaaaga aagaaaaaag
      961 agaaaagagg ccgatctgct ttcctctctt ctccatgcga aaatacagcg ggatccgccc
     1021 ggaggcggat cccgctgtat tttctttgcc tgttttctgc cggcggatta ctttgagaag
     1081 ccgagttcct tcgcttcgtc cttgtagcca agatcccaca ggaccgtacg gtagatctcg
     1141 aggaattcct tgccctctgc agacttgccg cactcttcga ccacgagctt gtgtcccaca
     1201 ctgcgggcct tggcttcgtc ctcggggctc cagcggacca cggtgacgtt gggcatttcc
     1261 ttccacttct cccgggcctt gatctcatgg acgtagacca tggcagagag atagcggatg
     1321 cgatcgcggg cgataatcac aatatccttg tactcctggg gaaggtcatt ccacctgtcg
     1381 ggattgacca ccagggggaa gtactcgcag gtcccgttct ggaaggcggg gtccacggcg
     1441 taggcggcca cttcgtgga
//
LOCUS       DAOTHD010000649         1458 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_104665_length_1458_cov_2.987883, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000649 DAOTHD010000000
VERSION     DAOTHD010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104665_length_1458_cov_2.987883"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="P5219_10085"
     CDS             complement(<1..600)
                     /locus_tag="P5219_10085"
                     /inference="COORDINATES: protein motif:HMM:NF014766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRX27154.1"
                     /translation="MPAISDVRIFRAVSPLSRPIADATHSLTEISFYVVEVETESGVT
                     GQGYLLSFHYSPHAISGALKDIRPLVLGLEPHETGVLAKRAAAEHEYFGCEGLQKWAL
                     AAANVAMWDCRGKMLGAPVWKLLGSTVSRVPVYGSGGWLSYTIEELLGEVTEYMRRGF
                     NAVKIKVGGGLEKDLERLRKVREAVGPGLKIMMDANQGMN"
     gene            complement(675..>1458)
                     /locus_tag="P5219_10090"
     CDS             complement(675..>1458)
                     /locus_tag="P5219_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRX27155.1"
                     /translation="DIVTAMPYTSCGKCWSCLCGRPNACKNNETLGVQREGSLTEFIV
                     LPWEKIVKGGTLGLRELAIVEPLAIGIHAGKRCDPKEGDCMLVFGCGMIGIGAVAEGI
                     RAGAKVIAVDVDDSKLEMMRSIGAAFTVNSKKEDLRERVLEITGGHGPAVVLEAIGLP
                     ETFVQAVDLAAYSGRVVFVGYAKAPVTFDTKYFILKEISIRGSRGSGRPDFEQAVALL
                     ESGAFPVDRLISAEFPFEKAGEAMEQWNANPGAVSRMMISLD"
BASE COUNT          252 a          504 c          412 g          290 t
ORIGIN      
        1 gttcatcccc tggttggcat ccatcatgat cttcagtccc ggcccgacgg cctcccggac
       61 cttccggagc cgttcgaggt ctttctccag gcctccgccc accttgatct tcacagcgtt
      121 gaacccccgg cgcatgtact ctgtgacctc gccaaggagt tcttcgatcg tgtaggaaag
      181 ccaccctccg ctgccgtaga cgggaacccg ggacacggtg ctccccagca gcttccacac
      241 cggcgccccg agcatctttc cccggcagtc ccacatggca acgttcgccg ccgcgagggc
      301 ccacttctgg agtccttcgc agccgaaata ttcatgctcc gccgcggccc gtttggcgag
      361 cacgcctgtt tcgtggggct ccagaccgag caccagggga cggatgtcct tcagggcccc
      421 ggagatggca tggggagaat aatggaagga cagcaggtac ccctgccccg ttacgccgct
      481 ctccgtctcc acttccacca cgtaaaacga tatttccgtc agcgagtggg tggcgtcggc
      541 aatggggcgg ctcagcgggg ataccgcccg gaatatgcgc acatcagaaa tggccggcat
      601 tctcttcttc ctttccttct ttctgcggga aggggccgtt tcttctcctc cccgcaggct
      661 gtttcgaaac tccgttagtc gagggagatc atcatccggg atacagctcc cggattggca
      721 ttccactgtt ccatggcctc gccggccttt tcgaagggga attcggccga aatgagcctg
      781 tccacgggga aggcgccgga ctcgagcaac gccacggcct gctcgaaatc gggacggccc
      841 gaccccctgg aaccccggat ggagatctct ttcaggatga aatacttggt gtcgaaggtc
      901 acgggagctt tcgcgtatcc cacgaaaacc acccgtcccg agtaagcggc gagatccacc
      961 gcctggacga aggtctccgg caggccgatg gcctccagca ccaccgccgg tccgtgtccg
     1021 ccggtgatct ccagtacccg ctcccgcagg tcctccttct tcgagttcac cgtgaaggcc
     1081 gcgccgatgg agcgcatcat ctcgagcttg gaatcgtcca cgtccacggc gataaccttc
     1141 gccccggccc ggattccctc tgcgacggca ccgatgccga tcatgccgca gccgaagacg
     1201 agcatacagt ccccttcctt cgggtcgcac cgcttgcctg catggatgcc gatggcaagg
     1261 ggctccacta tggccagttc ccggaggccg agcgtcccgc ccttcacgat cttttcccat
     1321 ggcaggacga tgaactccgt cagggatccc tccctctgca ccccgagggt ctcgttgttt
     1381 ttgcatgcgt tagggcgccc gcagaggcac gaccagcatt tcccgcagct cgtgtagggc
     1441 atggcggtga cgatgtcc
//
LOCUS       DAOTHD010000650         1455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_105007_length_1455_cov_2.833571, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000650 DAOTHD010000000
VERSION     DAOTHD010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105007_length_1455_cov_2.833571"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /gene="asrB"
                     /locus_tag="P5219_10095"
     CDS             complement(<1..779)
                     /gene="asrB"
                     /locus_tag="P5219_10095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HRX27156.1"
                     /translation="MKENPYRPFAAKITSLTPETDLEFTFTVEASVDMTPGQFFQVSL
                     PKIGEAPLSISAFGSDWIQFTVRAVGRLTNALQDLRKGEDLFIRGPYGVGFPLDVLQD
                     SNLVIVAGGSGTAPVRPLIRKALDGALPLRSLRVIAGFKNRESILFSGEVEEWRKRGD
                     VLVTIDKPQEGWNGPVGLVTEHIRKLALSDPGDVQFVVVGPPVMMKFAAAEVRLLGVP
                     EENIWVSFERLMSCGLGKCGHCKIDSTYICLDGPVLNFTKAS"
     gene            complement(776..>1455)
                     /locus_tag="P5219_10100"
     CDS             complement(776..>1455)
                     /locus_tag="P5219_10100"
                     /inference="COORDINATES: protein motif:HMM:NF028488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRX27157.1"
                     /translation="GGAADPYYRRRREKLYFFMIECRESFENCFCVSMGSNATDNYAA
                     AFRFGEDSVEVEVKDSSFVEFFAPGTPSSFAPEFVKENVKKVTVPSVEEMPPELFVSP
                     LWDEYDSRCIACGRCNTSCVTCTCFSSLDLFYSDSRNCGERRRVWDGCHLDGFSDMAG
                     GHSFRKKKGERMRFKTFHKIYDFRKRFGYDMCVGCGRCDDVCPEYISFSECIDRVSEA
                     LKGGEVQ"
BASE COUNT          304 a          449 c          442 g          260 t
ORIGIN      
        1 gaggccttcg tgaagttgag caccgggccg tcaaggcaga tgtaggtgga gtcgatcttg
       61 cagtggccgc acttgccgag gccgcaggac atgagccgct cgaaggagac ccagatgttt
      121 tcctccggta ccccaaggag acggacctcc gcagcggcaa acttcatcat cacgggaggg
      181 cccaccacca cgaactgcac gtctccaggg tcggaaaggg caagcttccg tatgtgctcc
      241 gtgaccagcc ccacgggacc gttccacccc tcctggggct tgtcgatggt gacgagcaca
      301 tccccccgct tccgccactc ctccacctct ccggaaaaga gaatgctctc ccggtttttg
      361 aatccggcga tcacccggag ggaccggagg ggaagggcgc cgtccagggc cttccggatc
      421 agggggcgga caggggccgt tccggagcct cccgccacga tgacgaggtt ggaatcctgc
      481 agcacgtcca gagggaaccc cacgccgtag ggcccccgga tgaagagatc ctctcccttc
      541 cgcaggtcct gcagggcgtt cgtcaggcgg cccacggccc ggacggtgaa ctggatccag
      601 tcggagccaa aagcgctgat ggagagaggg gcttcgccga tcttcggcag ggagacctgg
      661 aaaaactggc ccggcgtcat atcgaccgag gcttccaccg taaaggtgaa ctcgaggtcc
      721 gtctcgggag tgagggaagt gatcttcgcc gcgaacggcc ggtagggatt ctctttcatt
      781 ggacctctcc tcccttcagg gcttccgaga cccggtcaat gcactcagaa aaggagatat
      841 attccgggca gacatcgtcg caccggccgc accccacgca catgtcgtag ccgaagcgct
      901 tccggaaatc gtagatcttg tggaaggtct tgaaccgcat gcgctcccct ttcttcttcc
      961 ggaaggaatg gcctccggcc atgtcggaaa agccgtcaag atggcatccg tcccagaccc
     1021 ggcggcgctc accgcagttt ctgctgtctg aataaaacag gtcgagagag gagaagcagg
     1081 tacaggtgac gcaggaggtg ttgcacctgc cgcaggcgat gcaccggctg tcgtactcgt
     1141 cccagagagg agagacgaac aattcggggg gcatctcctc cacggaggga acggtcacct
     1201 ttttgacatt ctccttcacg aattccggcg caaaggagga cggggtaccc ggggcgaaga
     1261 attccacgaa actcgaatcc tttacttcca cctctacaga gtcctccccg aaccggaagg
     1321 cggctgcata gttgtccgtc gcattgcttc ccatggagac gcagaagcag ttctcgaagg
     1381 actcccggca ctcgatcata aagaagtaga gcttctctct cctgcggcgg taataggggt
     1441 cagcggcgcc gccgt
//
LOCUS       DAOTHD010000651         1455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_105042_length_1455_cov_2.025000, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000651 DAOTHD010000000
VERSION     DAOTHD010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105042_length_1455_cov_2.025000"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="P5219_10105"
     CDS             complement(<1..889)
                     /locus_tag="P5219_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRX27158.1"
                     /translation="MAKRELTAEQRSALEEIFAKARAAEKIIENYDQERVDRMCRCVA
                     WAAGNPRDFDRLCKMGVEESGAGDWSGRFGKRHKILGVLRDALRQKSVGVIEVLPEKG
                     LVRYGKPAGVITSLIPMTNPELTPIVTAIYALKARDVVVFSPHPRTKKTTFEVVEKMR
                     RGLRSIGEPEDFLQCISEVNMAVVEELMTMGDLIMATGGPAMTRAAHSSGKPAYCSGA
                     GNATMIFDETTDIEVAARNTRTSKTSDFGSGCSADGNLIIYGGIYDRMVAQLQKEGGY
                     LATDEQREMLKKAMWDDEGH"
     gene            complement(933..>1455)
                     /locus_tag="P5219_10110"
     CDS             complement(933..>1455)
                     /locus_tag="P5219_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRX27159.1"
                     /translation="PGGLATMGYGPSAARGVKVACPDKKVVALIGDGGMGANVSPFAT
                     AAVDDIAVVWVVMNNCAFGTIAGLENQHYQHQFGTLFEKDGKPYSPDFAAIGEAYGIK
                     GYTVKSASEFKPVLEEALALNKPCVLDVRMENAPVVTEGCWNINDIFRKRGEEKPWRV
                     WAWEETEPWRMEK"
BASE COUNT          231 a          471 c          431 g          322 t
ORIGIN      
        1 ggtgcccctc gtcgtcccac atggcttttt tcagcatctc ccgctgctcg tcggtggcaa
       61 ggtagcctcc ttccttctgg agctgggcaa ccattctgtc gtatattccg ccgtagatga
      121 tgaggttgcc gtcggcggag cagccggagc cgaagtcgga cgtcttgctg gtgcgggtgt
      181 tgcgggcggc cacctctatg tcagtggtct cgtcgaagat catggtggcg ttaccggccc
      241 ccgagcagta ggccggcttc ccggagctgt gggccgccct tgtcatggcc ggcccgcccg
      301 tggccatgat gaggtcgccc atggtcatga gctcctcgac gacggccatg ttgacctcgg
      361 aaatacactg gaggaaatcc tcgggctcac cgatggaccg gagcccccgg cgcatcttct
      421 ccaccacctc gaaggtggtc tttttcgttc tcggatgggg ggagaaaacc accacgtcac
      481 gggccttcag cgcataaatg gccgtgacga tgggagtgag ctccgggttg gtcatgggaa
      541 tgagggaggt aatgaccccc gcaggcttgc cgtaccggac aagccccttc tccgggagaa
      601 cctcgattac gccgacgctt ttctggcgca gggcatccct caggacaccg aggatcttgt
      661 gccgcttgcc gaacctgccg ctccagtcgc ccgccccgct ctcctccaca cccatcttgc
      721 agagcctgtc aaagtccctg gggttgcccg cggcccaggc cacgcagcgg cacatgcggt
      781 ccacccgttc ctggtcgtag ttttcgatga tcttctccgc ggcccgggcc ttcgcaaaga
      841 tctcttccag tgcgctcctc tgttcagccg tcagttccct tttcgccatg tatgtccatc
      901 tccttttctt tgtaagctcc cggaaaggag agctactttt ccatcctcca gggttcggtt
      961 tcttcccagg cccagaccct ccagggcttt tcctctcccc gcttcctgaa gatgtcgttg
     1021 atgttccagc atccttcggt aactaccggg gcgttttcca tccggacgtc gaggacgcag
     1081 ggtttgttca gggcgagggc ctcttcgagg accggtttga attcagaggc gctcttcacg
     1141 gtatatccct tgatcccgta ggcctcgccg atagcggcga agtcagggct gtagggcttg
     1201 ccgtccttct cgaagagagt gccgaactgg tgctggtagt gctggttctc gagcccggcg
     1261 atggttccga aagcacagtt gttcatcacc acccacacca cggcaatatc atccaccgcg
     1321 gcggtggcga agggggagac gttggcgccc atgccgccgt caccgatgag agcgaccacc
     1381 ttcttgtccg ggcaggccac cttcacgccg cgggcggcgg aaggaccgta ccccatcgtg
     1441 gcaagtccgc cgggg
//
LOCUS       DAOTHD010000652         1454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_105082_length_1454_cov_3.331665, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000652 DAOTHD010000000
VERSION     DAOTHD010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105082_length_1454_cov_3.331665"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="P5219_10115"
     CDS             complement(<1..642)
                     /locus_tag="P5219_10115"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HRX27160.1"
                     /translation="MKKLTALVLALVLFAGSAGAAFAAGNPMDYRGTIRIASQNVNET
                     IILAWMAKLLIDGHTGLDTTINTEFAGSAVLHQAMAADEIDLYPSWTGTQLTGILRYE
                     GESKPSDETFAMVKEGFEKNFNMTWTRPVGFNNTYVMAVKAERAEKYGLKKASDLADH
                     GPEWKLGGDENFDTRLDGYPGWSERYGITFRDVLPMQYAMMYMALAQEGVDVIA"
     gene            complement(672..1370)
                     /locus_tag="P5219_10120"
     CDS             complement(672..1370)
                     /locus_tag="P5219_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRX27161.1"
                     /translation="MMEQRPLWDLFITYVGRNYMRILNLTGEHIMISLIAVGITVMVC
                     VPLGIYLTRNEKAAPYVIGLANVFQTIPSLALLGFLIFVFGIGNDNAVCALFLYAMLP
                     VIQNTYTGIRSVPRSYVQAARGMGMTDFQILLKVQLPLARPVLVAGIRVAMVWTIGTA
                     TLASAIGGGGLGRLIFSGLSSIRNEIILAGAIPATLLALFADWILKRAQDYFNPSARA
                     VRLAARHGAADDTD"
BASE COUNT          262 a          428 c          470 g          294 t
ORIGIN      
        1 cgcgatgacg tccactcctt cctgggcgag ggccatgtac atcatggcgt actgcatggg
       61 aagcacgtcc ctgaaggtga tgccgtagcg ctccgaccag ccggggtatc cgtccagccg
      121 ggtgtcgaag ttttcatcgc cgcccagttt ccattccggg ccatggtccg ccaggtcgga
      181 ggcctttttg agaccgtatt tttcggccct ctccgccttc acggccatga cgtaggtgtt
      241 gttgaacccc accgggcggg tccaggtcat gttgaagttc ttctcgaagc cttccttcac
      301 cattgcaaag gtttcgtccg aaggcttcga ctcgccctcg taccggagaa ttcccgtgag
      361 ctgggtcccc gtccacgagg gatagaggtc gatctcgtcc gcggccatgg cctggtggag
      421 cacggcggaa ccggcgaact cggtgttgat ggtggtatcg aggcccgtgt gcccgtcgat
      481 gaggagcttg gccatccagg cgaggatgat cgtctcgttc acgttctgcg aggcgatgcg
      541 gatcgtcccc ctgtagtcca tggggttgcc ggccgcgaag gcggcgcctg cggatcctgc
      601 gaagagaaca agggcgagaa ccagtgctgt cagttttttc atggtgtcac tctccttttt
      661 tgaaattcgt ttcagtcggt gtcatccgcc gccccgtgcc ttgcggcgag ccgtaccgcc
      721 cgggcggagg ggttgaaata atcctgggcc ctcttgagaa tccagtcggc gaagagggcg
      781 agcagggtcg ccggtatggc gcccgcgaga atgatctcgt tcctgatgga ggaaaggccc
      841 gagaagataa gtctccccag cccgccgccg ccgatggccg aggcaagggt tgccgtgccg
      901 atggtccaga ccatggccac ccggatgccc gccacgagga cgggacgggc caggggaagc
      961 tggactttca gcagaatctg gaagtcggtc atccccatcc cccgggctgc ctgcacgtag
     1021 gatctcggaa cgctccggat gcccgtgtag gtgttctgga tcaccgggag catggcgtac
     1081 aggaagaggg cacagacggc gttgtcgttg ccgatgccga agacgaaaat gaggaacccc
     1141 aggagcgcca ggctcggaat ggtctggaac acgttggcga gcccgatgac gtagggagcc
     1201 gccttttcgt tcctcgtgag gtatattccc aggggaacgc agaccatgac cgtaatgccc
     1261 acggcgatga gggagatcat gatgtgttct ccggtgaggt tcaggatgcg catgtagttt
     1321 cttcccacgt aggtgatgaa aagatcccac agggggcgct gctccatcat gactgcttcc
     1381 cctcctccct gccgcttctg agccgttcca ccgccaggcg ggcaagggcg ccggacgtga
     1441 tgacgcccac aaag
//
LOCUS       DAOTHD010000653         1453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_105172_length_1453_cov_3.402003, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000653 DAOTHD010000000
VERSION     DAOTHD010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105172_length_1453_cov_3.402003"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            310..1155
                     /locus_tag="P5219_10125"
     CDS             310..1155
                     /locus_tag="P5219_10125"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRX27162.1"
                     /translation="MENMRVPARIVSGRRTTDGAGVSLVRVFGYHDVKDFDPFLMLDA
                     FDSKDPADYVKGFPWHPHRGIETITYLISGEMEHGDSLGNRGIIRDGDCQWMTAGSGI
                     IHQEMPKPSERLLGAQLWLNLPAKDKMCPPKYNDLVSGNIPRITEGTAEVRVLSGTYR
                     GVEGPMKAHYVQPRYLDVSVRGGGRWTVETPPEATVFVYIVEGEGAFGPGNGTEVKEK
                     NAVLFSRGKGFSVQAAGGGIRFLYLEGMPLGEPVAWGGPIVMNTKEELDQAFRDLDNG
                     TFIRK"
     gene            1192..>1453
                     /locus_tag="P5219_10130"
     CDS             1192..>1453
                     /locus_tag="P5219_10130"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRX27163.1"
                     /translation="MDVVIQAGILVFPDVEELDFVGPFETLLAANVVKPGSMDVKILA
                     FQDGPIRAANGLRFLPDGGPDLCPQLDVLILPGGQGRRIAVRD"
BASE COUNT          317 a          394 c          464 g          278 t
ORIGIN      
        1 gggcatccac cgtcgggact ccagtggggc ctcctcccag cacctggtgc cggtacccca
       61 cccaggtgat gaatgccaga aaaccgaaca tgaccgcata ccggacccgc ttccacgtga
      121 acacggcaat cgcctcctcc gaaaaagtgg aaagagaata acacccatgg gacaggaata
      181 ctattggaag attatacccc tcaggagtac gtatttctgc tgatcttctg cgggttgacc
      241 tggccttgac cgatctcctt cctttgaata gtataacgta ataaagagat gaggaggtgt
      301 tctttaaaaa tggagaatat gcgggttccg gccagaatcg tttccggccg caggacgacg
      361 gatggagcag gagtcagcct cgtgcgcgtc ttcgggtatc atgacgtgaa ggacttcgat
      421 cccttcctca tgctcgacgc ctttgactcg aaagaccccg ccgactatgt aaagggcttt
      481 ccgtggcatc cccacagggg catcgagacg atcacctacc ttatcagcgg agaaatggaa
      541 cacggggaca gcctcggcaa caggggaatc atccgcgacg gtgactgcca gtggatgacc
      601 gccggctcgg gcatcatcca ccaggagatg ccgaagccgt cggaacggct tctcggcgcc
      661 cagctctggc tcaaccttcc ggcgaaggac aaaatgtgcc cgccgaagta caatgatctt
      721 gtcagcggga atattccacg gataacggag gggacagcgg aggtccgggt cctgtccggg
      781 acgtacaggg gagtggaggg ccccatgaag gcccactacg tgcagccacg gtatctcgac
      841 gtttccgtcc gtgggggcgg gagatggacg gtggaaactc ctcctgaggc cacggtgttc
      901 gtgtacatcg tggaggggga gggtgccttc ggtccgggaa acggaactga ggtgaaagag
      961 aaaaacgccg ttcttttctc ccgggggaag gggttttcgg tgcaggctgc cgggggggga
     1021 atccggttcc tctacctgga gggcatgccg ttgggggaac ctgtcgcctg gggcggcccc
     1081 atagtgatga acaccaagga ggagcttgac caggccttcc gggacctgga caatggaacg
     1141 tttatccgga agtgaaacgg actccgggag tacacattcg gaagagagag ggtggatgtc
     1201 gtgatacagg caggaatact tgtttttccc gacgttgagg agcttgattt tgtcggcccc
     1261 ttcgagacgc tgttggcggc gaacgtggtg aagccgggca gcatggacgt gaaaatactc
     1321 gccttccagg acggacccat cagggcagcg aacggtcttc gcttcctgcc ggacggaggg
     1381 cccgatctct gcccgcagct tgacgtgctc atccttccgg gagggcaggg ccgccggatc
     1441 gccgttcggg aca
//
LOCUS       DAOTHD010000654         1452 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_105274_length_1452_cov_3.255548, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000654 DAOTHD010000000
VERSION     DAOTHD010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105274_length_1452_cov_3.255548"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..114)
                     /locus_tag="P5219_10135"
     CDS             complement(<1..114)
                     /locus_tag="P5219_10135"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX27164.1"
                     /translation="MVKEAVENSLDAGATEVRVSLYEGGKVRIVVEDNGEGI"
     gene            complement(167..>1452)
                     /gene="mutS"
                     /locus_tag="P5219_10140"
     CDS             complement(167..>1452)
                     /gene="mutS"
                     /locus_tag="P5219_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRX27165.1"
                     /translation="WMDAYLEKIRTETGISKIKAGYNKVFGYYLEVSKGSLGSVPDFF
                     IRKQTLVNAERFITEEMKVFEERMESASAEISRREGELYDRLVGETLDRVEDLQALGE
                     TLAVLDVLASLAETAREKGYVRPAVNSGFDLVVKNGRHPVVEDVLTDSPFVPNSVALE
                     GDGKRIALITGPNMAGKSTYLRSAALLVILAQMGSFVPAEAAEIGLCDRIFTRIGARD
                     DLARGSSTFMVEMVETANILHNVTDRSLVILDEVGRGTSTYDGMSIAWAVLEYLADGC
                     GASPKVLFATHYHELTCLEDRFPCMENLSMAVKESEGGVFFLHQVVKGSADRSYGIEV
                     ARLAGLPRPVLRRAFDLLQRFEKEERTGNFPKKKREVSGARQMDLFEAERHGIVEELA
                     AIEPDGLTPFRALELLYKLSEKSREVLRPENHASS"
BASE COUNT          244 a          513 c          415 g          280 t
ORIGIN      
        1 gattccttcg ccgttgtcct ccaccacgat gcggaccttc cccccctcat agagggagac
       61 cctcacttcc gtggcgccgg cgtcgaggga gttctccacc gcctccttga ccacggacgc
      121 gggacgctcc accacttccc ccgccgcgat gcgggagaat acatcctcag gaagaagcgt
      181 gattttcagg acgaagcacc tccctgctct tctcgctcag cttatacagc agctccaggg
      241 ccctgaaggg cgtcaggcca tcgggctcga tggcggccag ttcctccacg atgccgtgcc
      301 gctcagcttc gaacaggtcc atctgccggg ctcccgaaac ttcccgcttc ttcttcggga
      361 aattccccgt ccgctcttct ttctcgaacc gctgcagaag gtcgaaggcc ctccggagca
      421 cgggacgggg cagcccggca agccgggcca cttcgatgcc ataggaccgg tcggcgctcc
      481 ccttcaccac ctggtgaagg aagaagacac ccccttcgct ctccttcacc gccatgctca
      541 ggttctccat gcaggggaac cggtcctcga ggcaggtcag ctcgtggtaa tgggtcgcga
      601 aaagcacctt cggcgaggcg ccgcacccgt cggccaggta ttccagcact gcccaggcga
      661 tgctcatgcc gtcgtaggtg gaggttcccc gccctacttc gtccaggatc accaggctcc
      721 ggtccgtgac gttgtggagg atgttggccg tctccaccat ctccaccatg aaggtgctgc
      781 tgccccgggc gaggtcgtcc ctggcaccta tgcgggtgaa gatccggtcg cacagcccga
      841 tctccgccgc ctcggccgga acgaagctcc ccatctgggc caggatcacc agcagggcgg
      901 cgctccgcag gtaggtggac ttccccgcca tgttcggccc ggttatcagg gcgatgcgct
      961 tcccatcccc ttccagcgcc acggagttgg gaacgaaggg cgaatcggtg agcacatcct
     1021 ccaccacggg gtggcggccg ttcttcacaa caaggtcgaa tccggagttc actgcaggcc
     1081 ggacgtaccc cttctcccgg gcggtctccg ccagggaggc cagcacgtcc aggaccgcga
     1141 gggtctcacc gagggcctgg aggtcctcca cccggtccag ggtttccccg accagccggt
     1201 cgtagagttc cccctccctc cgggagattt cggcggaggc tgactccatc ctctcctcga
     1261 agaccttcat ctcctccgtg atgaaccgct cggcgttcac cagggtctgt ttcctgatga
     1321 agaaatccgg caccgagccc agggagccct tgctcacctc gagatagtag ccaaagacct
     1381 tgttgtaccc cgctttgatt ttggaaatgc ccgtttcggt ccggatcttt tccaggtagg
     1441 cgtccatcca gg
//
LOCUS       DAOTHD010000655         1450 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_105470_length_1450_cov_3.234409, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000655 DAOTHD010000000
VERSION     DAOTHD010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105470_length_1450_cov_3.234409"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="P5219_10145"
     CDS             <1..363
                     /locus_tag="P5219_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRX27166.1"
                     /translation="HERHGRPVCLPVIEIRPRGGFYDYGSKYTPGGSEYRVPAPLEES
                     VRKAVEQAALGAYEALGCRAYGRVDLRLDDDGTPVVLELNTVPGMTATSLVPKAAAAA
                     GISFGEFLGMVVESSLSR"
     gene            complement(370..>1450)
                     /gene="pfp"
                     /locus_tag="P5219_10150"
     CDS             complement(370..>1450)
                     /gene="pfp"
                     /locus_tag="P5219_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HRX27167.1"
                     /translation="VSRIHSDGGSILKTSRFNPTKSDEHLRRVVDTLIELGVTHLVTI
                     GGDDTAYAASRIAGYAKNSLGLTISFAHVPKTIDNDLPLPEGIPTFGFETARSLGAQL
                     VSNLMEDAKTTGRWFLVVAMGRVAGHLALGIGKSAGATITIIPEEFSPNEKIPLSLVV
                     DIITGSIIKRLASGRNYGVAVTAEGLIERIRIEDLEAADCLEYDDHGHIRYAEINFSD
                     VLKKELLANLNSLGIRMTVNNKEVGYEVRCAPPNAFDIEYTRNLGYAAFDFLRSGGTN
                     ALITIQNNMIVPISFDDILDPVTKKTRIRTVNTESIQYRIAREYMIRLEKRDFQGGID
                     FEKLAVAAKLPPDEFRRRFQYVTEY"
BASE COUNT          232 a          425 c          498 g          295 t
ORIGIN      
        1 catgagcggc acgggaggcc cgtctgcctc ccggtcatcg agatccgccc caggggcgga
       61 ttttatgact acggatcgaa atacaccccc ggggggagtg aataccgtgt tccggccccc
      121 ctggaagaat ccgtcaggaa ggccgtggag caggctgccc tcggagcgta cgaggccctg
      181 gggtgcaggg cctacggcag ggtggacctc cgtctcgacg acgacggcac gcccgtggtg
      241 ctggagctca acacggtacc ggggatgacc gccacaagcc tggtgcccaa ggcggcggcg
      301 gcggcgggca tctccttcgg ggagttcctc gggatggtgg tggaaagctc cctgtcccgc
      361 tgaggctcct cagtattcgg tcacgtactg gaatcgcctg cggaactcgt cgggggggag
      421 cttcgcggcc acggccagct tctcgaaatc gatgcctccc tggaagtccc gcttctccag
      481 ccggatcatg tactccctgg cgatcctgta ctggatcgat tccgtgttca ccgtgcggat
      541 gcgcgtcttt ttcgtcaccg ggtcgaggat atcgtcgaag gagatgggga cgatcatgtt
      601 gttctgtatg gtgatgaggg cgttggtgcc gccgctccgg aggaagtcga aggcggcgta
      661 gccgaggttc cgggtgtact cgatgtcgaa ggcgttgggc ggggcgcacc ggacctcgta
      721 gcctacctcc ttgttgttga cggtcatcct gatcccgaga gaattgaggt tcgccagcag
      781 ctcctttttc agcacgtcgg agaaattgat ctccgcgtag cggatgtgcc cgtggtcgtc
      841 gtattccagg cagtcggccg cctccaggtc ctcgatccgt atcctctcga tgagaccctc
      901 cgccgtcacg gccacgccgt agttccttcc cgaggcgagg cgcttgatga tggagcctgt
      961 gataatgtcc actaccaggg aaagggggat tttttcgttc ggggaaaatt cctcggggat
     1021 gatggtgatg gtggcgccgg cgctcttgcc gatgccgagg gcgagatggc ccgccaccct
     1081 tcccatggcc accacgagga accacctgcc ggtggtcttg gcgtcctcca tcaggttcga
     1141 cacgagctgg gcccctagtg agcgggcggt ctcgaaaccg aaagtcggga tgccctccgg
     1201 gaggggaagg tcgttgtcga tggtctttgg gacgtgggcg aagctgatgg tgagtcccag
     1261 gctgttcttc gcgtagcccg cgatgcggga cgcggcgtag gcggtgtcgt cgcccccgat
     1321 ggtcaccagg tgggtgaccc cgagctcgat gagggtgtcc accacccggc gcagatgttc
     1381 gtcgctcttg gtgggattga aacgggaggt cttcaggatg ctgcccccgt cggagtgaat
     1441 ccggctcacc
//
LOCUS       DAOTHD010000656         1448 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_105668_length_1448_cov_3.164393, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000656 DAOTHD010000000
VERSION     DAOTHD010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105668_length_1448_cov_3.164393"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1448)
                     /locus_tag="P5219_10155"
     CDS             complement(<1..>1448)
                     /locus_tag="P5219_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRX27168.1"
                     /translation="LNMGNLVAGTKYRGEFEERMRKLVKELSEARDVILFIDEIHTIV
                     GAGGAEGAVDAANILKPSLSRGEFQVIGATTLDEYRKHIEKDAALERRFQPVHVQEPS
                     VDDSILILEGLRDRYESHHRAKIQDDALEAAARLSARYITDRHLPDKGIDLIDEAAAR
                     ARINAMETPESLKEMEKKLEEIRREKEAAVDSQEFEKAARLRDDERKLSEDLEEERRK
                     WRSRRNSYQPVVTGEDIADVVAEWTGIPVFQLTGEESERLLRMEEEIEKRLIGQNEAV
                     VSVARAIRRARSGLKDPRRPIGSFLFLGPTGVGKTELARRLAEFLFGSQDTMIRLDMS
                     EFMEKHEVSKLIGAPPGYVGYEEGGKLTEAVRRRPYSVVLFDEIEKAHPDIFNILLQI
                     LEDGRLTDGQGRTVDFRNTVILMTSNVGAREAARGSTLGFSGGDEKDPFDWSRLKAAI
                     MDEVNRTFRPEFINRLDEMIVFRPLNRENMIS"
BASE COUNT          258 a          460 c          379 g          351 t
ORIGIN      
        1 tggatatcat gttctccctg ttcagcggcc ggaagacgat catttcatcg agacggttga
       61 tgaactcggg ccgaaatgtc ctgttcacct catccattat cgcggccttg agcctgctcc
      121 agtcgaaggg gtctttctcg tctcccccgg aaaacccgag agtcgacccc ctggccgcct
      181 ccctggcccc gacgttgctg gtcatgagga tgaccgtgtt ccggaaatcc acagttctcc
      241 cctgaccgtc cgtcagccgt ccgtcttcca gaatctggag cagtatgttg aatatgtcgg
      301 gatgggcctt ttcgatttcg tcgaaaagca ccaccgagta gggtctccgc cggaccgctt
      361 ccgtgagctt gcctccctct tcgtagccaa cgtatcccgg gggggcgccg atgagtttgg
      421 aaacttcgtg tttttccatg aattcgctca tgtcaagacg gatcatggta tcctgcgaac
      481 cgaagagaaa ctcggccagc cgcctggcaa gttccgtctt gcccactccg gtgggcccga
      541 ggaagaggaa gctgccgatg ggccgcctgg ggtctttgag cccgctcctg gcacgccgta
      601 tggccctggc gacggaaacc acagcttcat tctggccgat gagccgcttc tcaatctcct
      661 cttccattcg gaggagccgc tccgattcct ctcccgtaag ctggaaaacc ggtattccgg
      721 tccattcggc caccacgtcg gcaatatcct cacccgttac cacaggctgg taggagtttc
      781 tccgggaccg ccacttccgc cgctcttcct caagatcctc cgaaagcttc ctttcatcat
      841 ccctgagccg ggcagccttt tcaaactcct gggaatcaac ggcagcttcc ttttccctcc
      901 tgatctcctc gagtttcttc tccatctctt tcagggattc aggggtctcc atggcattga
      961 tccttgccct ggcggccgcc tcgtcgatga gatctatccc cttgtccgga agatgacggt
     1021 cggtgatgta ccgggcggaa agccgtgcgg cagcctcaag ggcatcgtcc tgtatcttcg
     1081 ccctgtgatg ggattcatac cggtcacgaa ggccttcaag gataagaatg gagtcgtcaa
     1141 cgctcggttc ctggacatga acgggctgaa aacgtcgctc aagggcggcg tccttttcga
     1201 tatgcttccg gtattcgtcc agcgtagtgg ccccgatgac ctgaaactct ccccgggaaa
     1261 gacttggctt gagaatgttg gcggcatcaa cggccccttc tgcccctccc gctccgacaa
     1321 tggtgtggat ctcgtcgatg aagaggatga cgtccctggc ctcggacagt tccttcacca
     1381 gttttctcat gcgctcctcg aactctcccc ggtacttggt tcctgccaca aggttcccca
     1441 tgttgagc
//
LOCUS       DAOTHD010000657         1447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_105762_length_1447_cov_3.860632, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000657 DAOTHD010000000
VERSION     DAOTHD010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105762_length_1447_cov_3.860632"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..358)
                     /locus_tag="P5219_10160"
     CDS             complement(131..358)
                     /locus_tag="P5219_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27169.1"
                     /translation="MDWGRVPADTMVVESKNITLRDVVNAAANGVDTAEDLMEHLGLE
                     EGEAGTEQLQPILDVFLPAIERLRSGSCGGG"
     gene            complement(505..1311)
                     /locus_tag="P5219_10165"
     CDS             complement(505..1311)
                     /locus_tag="P5219_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27170.1"
                     /translation="MKRLCCPLFLAMFLLFTSAAAAADFTGIWAFSGKDGTEYSFDLL
                     QNGSVLEGTFSAYDSGLDKYREAGIEGSAGGRIATVAVNGGRGILTMDEEGLSVEWTL
                     TSVGKEKILPPSGTVLPRDFAAIGFRILSGEKLFVLEDAETEKEILKLLGKPEKKTKA
                     EEWGADGLIHQKWLYGKMGIAVDFVREGTSGTGKADSVTLSAPSRKKTSRGIGIGSLQ
                     KEVLEAYGAEFNREESLLPDTIIAGSVFGGLVFTMKDGRVESIFIGAAAE"
BASE COUNT          291 a          450 c          364 g          342 t
ORIGIN      
        1 cggaaggagc ccccgcccct tccgattgaa gtgaagggcg aaaaaaaaag cccggaagtc
       61 cccttgaagg gtgcttccgg gcttttttcc cgtctttgat cagcagctaa cagctgccgg
      121 agcagccggc tcacccgccg ccgcagcttc cgctccggag gcgttcgatg gccggaagaa
      181 acacgtcaag aatgggctgc agctgttccg ttcccgcttc gccttcctcg agtccgaggt
      241 gctccatgag atcctcggct gtatcgacgc cgttggccgc ggcattgacc acgtccctga
      301 gggtgatgtt cttgctctcc accaccatgg tgtcggcagg cactcttccc caatccatga
      361 gtaattcctc cttccgttcg aaaatcggcc cttgttccga aaaagggtcc tccggaagaa
      421 aagccggagg tattataccc tatttcggta tttccgccat cggttctttc gcagtccgaa
      481 atttcttgtc aggaaactca atccctactc cgccgccgct ccgataaaga ttgattccac
      541 gcggccgtcc ttcatggtga agacgagtcc cccgaagacc gaaccggcga tgatcgtatc
      601 cggcagcagg ctctcttccc ggttgaactc cgcaccgtag gcttccagta cctctttctg
      661 cagactcccg atccctatgc cgcggctagt cttttttcgg gacggcgctg aaagggtcac
      721 cgaatcggcc ttgccggtac ctgacgttcc ttccctgacg aaatcgacgg cgatccccat
      781 tttcccgtac aaccatttct gatgaataag gccgtcggcg ccccactctt cggctttcgt
      841 ttttttctca ggtttgccga ggagcttcag gatttccttt tccgtctctg catcttccag
      901 aacgaagagt ttctccccgg aaaggatcct gaagccgatg gcggcgaagt ccctgggcaa
      961 tacggtgccg gacgggggca gaattttttc ttttcccacg ctcgtgagcg tccattcgac
     1021 cgaaaggcct tcttcatcca tcgtcagaat tcccctcccg ccgttcactg caacagttgc
     1081 gattctcccc cctgctgatc cttcaatgcc ggcttcccgg tatttatcca gcccggaatc
     1141 gtaggcggaa aaagtacctt cgagcactga accgttctgg agcaggtcaa aggagtactc
     1201 cgtcccgtct ttcccggaaa aggcccatat tcccgtaaag tctgcggctg cggccgcaga
     1261 ggtgaaaaga agaaacatcg cgaggaaaag cggacagcat aagcgtttca aagtgtattc
     1321 ctcccaggag aatgagttct gaaaaaccgg aagagttctg cagaggcttc tcccgacgat
     1381 cgaattatac tcctgttttc cggcccggct cacaccgtgc cgtttcctgc actggggatt
     1441 ccggagc
//
LOCUS       DAOTHD010000658         1447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_105792_length_1447_cov_2.870690, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000658 DAOTHD010000000
VERSION     DAOTHD010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105792_length_1447_cov_2.870690"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="P5219_10170"
     CDS             <1..380
                     /locus_tag="P5219_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRX27171.1"
                     /translation="TGHGKRHDAYEILYCPDGEITEAAHSTFFLYKGGTLVTAPLSRV
                     LKGTTRDIILQLAREKGMKVEERCPLLSELPEADEAFITGSVKEVVPVVRIGDQAVGS
                     GEPGPVTRMLHHTFLEEIIRWLE"
     gene            complement(361..>1447)
                     /locus_tag="P5219_10175"
     CDS             complement(361..>1447)
                     /locus_tag="P5219_10175"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: protein motif:HMM:NF013991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HRX27172.1"
                     /translation="EFEEGHPRGCLADGDLMDSLRGETGRKSLGLPPRRCLVCGRPAA
                     ECVAGRTHGTGEVRASVDRLLSVPGGVPGERPVSAIGETARKAVLFETVAFPKPGLVD
                     PLSRGAHGDMDCFTFLASSAALAPVWERFARLGFRFGGDDPAGLLPSLREEGIRAERM
                     MFSATGGINTHKGLIFSLGILCASAGMIASEGIPLSPEVCASRGAAIVRGITGRDFAS
                     LKGTADGRPLTAGERFYLSDGVTGIRGEAERGFPSVTGTGLPSLRSSLAKGLSLNDAM
                     VDGLLALMTVVEDTNILSRGGREGERIVRENAVYALELGGMGSKEGKEAVREMNGLFT
                     RCNLSPGGSADLLAVTVFLHLLTPASG"
BASE COUNT          286 a          491 c          440 g          230 t
ORIGIN      
        1 acacggggca cggcaagcgc cacgacgcct acgagatcct ttactgcccc gacggggaga
       61 tcaccgaggc ggcccacagc acgttcttcc tttacaaggg aggaacgctg gtcacggccc
      121 ccctgtcgag ggtgctcaag ggaaccacga gggacatcat cctgcagctg gcacgggaaa
      181 agggcatgaa ggtggaggag cggtgccccc tgctctccga gcttccggaa gccgacgagg
      241 ccttcatcac cggatcggtg aaggaagtgg tgcccgtggt gcggatcggc gaccaggcgg
      301 ttggctccgg ggaacccggc ccggtcacca ggatgctcca tcataccttc ctggaggaaa
      361 tcatccgctg gctggagtga gcaggtgaag aaacaccgtc acggccaaaa gatccgcgct
      421 tccgccggga ctgagattgc agcgagtgaa gaggccgttc atttcccgga cggcctcttt
      481 tccttccttc gaccccatgc cgcccagttc gagggcatag accgcattct ccctgacgat
      541 ccgctcccct tccctgcccc ccctggagag aatgttcgta tcctccacca cggtcatgag
      601 ggcaagcagt ccgtccacca tggcgtcatt cagggaaagg cccttcgcca gtgatgaacg
      661 gagagaggga agacccgttc cggtcaccga gggaaagcct ctttccgcct ctccccggat
      721 gcccgttacc ccatcgctca ggtagaaccg ctcacccgcc gtgagagggc gcccgtcagc
      781 cgttcccttc agggaggcga aatcccgccc ggtgatgccc cggacgatgg cggctcccct
      841 ggaggcgcac acctccggcg aaaggggaat tccttcagaa gcgatcatgc ctgccgacgc
      901 acagagaatc cccagggaaa aaatcagccc cttgtgggta ttgattcccc ctgtggcgga
      961 gaacatcatc cgctcggccc gaattccttc ctcccggaga gagggaagaa gcccggcagg
     1021 gtcatcccct ccgaaacgga atcccagccg ggcaaagcgc tcccataccg gggcaagggc
     1081 cgccgaggac gccaggaatg taaagcagtc catatccccg tgggctcccc tggacagggg
     1141 atccacgagt ccgggcttgg gaaaggcaac cgtttcgaac agcaccgcct tccgggccgt
     1201 ctcgccgatg gcggagacgg gtctttctcc cggaacgccg ccgggaacgg aaagaagacg
     1261 atccaccgac gcccggactt ccccggtgcc gtgagtcctg cccgcaacac attccgcagc
     1321 cgggcgtccg cagacgaggc accgtctcgg cggaaggccg aggcttttcc tcccggtctc
     1381 gccgcggaga gagtccatga ggtcgccgtc ggccaggcac cccctggggt gcccttcttc
     1441 aaactcc
//
LOCUS       DAOTHD010000659         1447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_105813_length_1447_cov_2.410920, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000659 DAOTHD010000000
VERSION     DAOTHD010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105813_length_1447_cov_2.410920"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="P5219_10180"
     CDS             <1..871
                     /locus_tag="P5219_10180"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRX27173.1"
                     /translation="LRIFTYHDRHTYPVLPSGLGDGWVHYQGLKIYLDGSLGGRTAAL
                     SAPYSDDPWEEGCLNWADGDVLELLREARRRGVQTMLHAIGDRGLDQGLRCIGAVDGE
                     SGVPALRDRINHVMVCRPDQRRRLASLGLFCDIQPAFVPSDMNMAESRLGRERLSWAY
                     GWRSLLDEGLVISASSDAPVESVNPWLSVRHLLDRAGNGGRDVFRPEERFSLEEALPL
                     FTSNPWQAIGRGTFSGRLASGYAPTSPSSTGTSRGPTAVSWERPPPSSSLREESCQKE
                     NWTAFPGFPADCR"
     gene            1151..>1447
                     /locus_tag="P5219_10185"
     CDS             1151..>1447
                     /locus_tag="P5219_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27174.1"
                     /translation="MMIRKSRRGCVSFLMVFSCLLAFTSFAEGAVKRVTVNGGIAPRD
                     GNSWEKAFSVEEFRINHAMMPVDTEFWLAAGVYAPGTAGNRDAAFYLASGTSIYG"
BASE COUNT          264 a          428 c          458 g          297 t
ORIGIN      
        1 cttaaggatc ttcacctacc atgacaggca cacctatcct gttctgccct cgggtctggg
       61 agacggctgg gtccattacc aggggctcaa gatctacctc gacggaagcc tcggcgggag
      121 gacggccgcc ctttcggccc cctattccga cgatccctgg gaggaggggt gcctcaactg
      181 ggctgacggc gacgtgctgg aactgctccg ggaggcacgg aggcggggcg tccagaccat
      241 gctccacgcc atcggcgacc ggggcctcga ccaggggctc cggtgcatcg gggccgtgga
      301 cggcgaatcc ggtgttccgg ctctccggga caggatcaac cacgtgatgg tctgccgtcc
      361 tgaccagcga cggaggctcg cctccctcgg gctcttctgc gacatccagc cggcctttgt
      421 ccccagcgac atgaacatgg cggaaagccg cctgggcagg gagcggcttt cctgggccta
      481 cggctggcgg tccctgctgg acgaggggct cgttatttcc gcgtccagcg acgccccggt
      541 ggaatcggtg aacccatggc tctccgtccg gcacctcctg gaccgtgcgg gcaacggcgg
      601 acgagacgtc tttcgtccgg aagagaggtt ttccctggag gaggcgctgc ctcttttcac
      661 ctccaaccca tggcaggcaa tcgggagggg aaccttctcc ggcaggctcg cttcgggata
      721 tgccccgacg tcaccgtcct cgaccgggac atctcggggg ccgacggcag tgtcgtggga
      781 gaggcctccc ccctcttcgt ccttgcggga ggaatcctgt caaaaggaga actggacggc
      841 gtttcccggt tttcccgctg actgcagata gaactggaag tcccggagcc gccgagcggt
      901 tccggggctt tttcgcatct cccggccggg atggagtgcg ctgaaaggat caaaggggtg
      961 agttcccagt accctcattg tttgcggtgt atcaaaaatc cgtgcggaga cgggttttga
     1021 gagattattt ttccagcaaa gaggttcttc tcaccctatt tacaaaaacg atacagacaa
     1081 tttattcttc agtcagcaac ggctcccggt tgttttcgac gcgacgaatt ccgcagaagg
     1141 aggtacagca atgatgatca gaaagagcag acggggatgt gtttcgtttc tgatggtgtt
     1201 ttcgtgcctt ctggccttca cttctttcgc cgaaggggcg gtaaagcggg tcacggtgaa
     1261 cggggggatt gcccccaggg atggaaattc ctgggaaaaa gcctttagtg tcgaagagtt
     1321 ccggatcaac cacgccatga tgcctgtcga cacagaattc tggctggccg ccggcgtcta
     1381 tgcaccaggg acggcgggaa acagggacgc cgctttttac cttgcctccg gcacgtccat
     1441 ctacggg
//
LOCUS       DAOTHD010000660         1447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_105822_length_1447_cov_2.200431, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000660 DAOTHD010000000
VERSION     DAOTHD010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105822_length_1447_cov_2.200431"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="P5219_10190"
     CDS             complement(<1..933)
                     /locus_tag="P5219_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRX27175.1"
                     /translation="MVSRGELQRRHRLLREAMESEGIDALVAGASAQLDQRGLLRWLF
                     DYYLPVFEEYAVIPLDGPAVFFAHNGGSASHAAQCFLVNDVRVIPPKDWNFDRGRLPA
                     DFIRNHGFKKVGIAGTAGISAVFFRSFLARLSVLPADFSDRAAESRMVKSDEEIRLIK
                     KTIELNEATLKVYLDHAEAGKPECEAMNAASCFAAEAGAEDQFWMEGTGTDGPPSPSP
                     GALRTGRCRQKGDYAVVVIEHSAPGGYFGEVVQTIRTGKRDSSLEEAHSAVIEAIRSA
                     SGMIRPGNRVGDVAAAAERTLIERGYPVSPTGDGS"
     gene            complement(946..>1447)
                     /locus_tag="P5219_10195"
     CDS             complement(946..>1447)
                     /locus_tag="P5219_10195"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRX27176.1"
                     /translation="TALFRIFVDASVSSAIIMFIMGCAGAFTWILTTSAVAAKLSAAL
                     LSVSANNTVMMLIIMVIFMIAGCFVDSASGFYLLLPIILPIVKKTGYPMYAFGVLATV
                     NFAIGQITPPVGSNLFVACNIADVTMRDLVSKVWPFLIAGIVCLLLMTFFPQIITCLP
                     SLLGLS"
BASE COUNT          315 a          420 c          429 g          283 t
ORIGIN      
        1 ggaaccgtct ccggtggggg acacagggta tcccctttcg ataagggtcc gttcagccgc
       61 cgccgccaca tctcccaccc tgttccccgg gcggatcatt ccggaagcag agcggatggc
      121 ctcaatcacg gcggaatggg cctcctccag gctgctgtcc cttttcccgg ttctgatggt
      181 ctgtacgacc tcaccgaagt accctcccgg tgcggaatgt tctatgacca cgacggcata
      241 gtcgcctttc tgccggcatc tcccggtccg cagtgccccg ggagaggggg aaggaggtcc
      301 gtccgttcct gtcccctcca tccaaaactg gtcctcggcg cctgcttccg ccgcaaagca
      361 ggatgccgcg ttcatggcct cacattcagg ctttccggcc tccgcatgat cgagatagac
      421 ttttagggtc gcctcgttca gttcgatggt tttttttatc agcctgattt cctcatcgct
      481 ctttaccatc ctgctctccg ccgccctgtc ggaaaagtcc gctggaagga cggaaagacg
      541 tgcgagaaag gacctgaaga atacggccga aatccctgcc gtacccgcga ttcccacttt
      601 tttgaagcca tggttccgga tgaaatccgc aggaagccga ccccggtcaa aattccagtc
      661 tttcggcgga atgacgcgga cgtcgttcac caggaagcat tgtgccgcat gggaggcgct
      721 tcccccgttg tgggcaaaaa aaacagccgg tccgtccagg gggatgaccg catattcttc
      781 aaagaccggg aggtagtaat cgaacagcca tcgtagcagt ccccgctggt cgagctgggc
      841 cgatgcccct gcgacaaggg catcgatacc ttccgattcc atggcctccc tgaggagcct
      901 gtgccgccgc tgcagttctc ccctgctgac catcggggag cctcctcagc tgagcccgag
      961 aagggagggc aggcaggtga ttatctgcgg gaagaaagtc attaggagca gacagacgat
     1021 tcccgcaatg aggaagggcc ataccttcga cacaagatcc cgcatggtca cgtcggcaat
     1081 attgcaggcg acgaacagat tggacccgac gggaggggtg atctggccga tggcgaaatt
     1141 gaccgtcgcc aggacgccga aggcgtacat ggggtacccg gtcttcttga ctatgggcag
     1201 gatgatggga aggaggaggt aaaaaccgga agcggagtcg acgaagcatc ctgcgatcat
     1261 gaaaatcacc atgatgatga gcatcattac agtattattg gccgatacgg agagaagagc
     1321 ggccgagagt ttggcggcca cggcggacgt ggtgagtatc cacgtgaagg ccccggcaca
     1381 tcccatgatg aacatgatga tagccgacga cacggaggcg tccacaaaaa tgcggaaaag
     1441 ggcggtt
//
LOCUS       DAOTHD010000661         1445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_106001_length_1445_cov_2.441727, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000661 DAOTHD010000000
VERSION     DAOTHD010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106001_length_1445_cov_2.441727"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="P5219_10200"
     CDS             complement(<1..262)
                     /locus_tag="P5219_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27177.1"
                     /translation="MAVWTFDGTFNGFLCLIHESSRRKERPEGILGGGKNQPFLFETV
                     PTETDEGLAASVRTALAARLSRRVLTEGYYAFLSAMPGREMAV"
     gene            complement(265..>1445)
                     /locus_tag="P5219_10205"
     CDS             complement(265..>1445)
                     /locus_tag="P5219_10205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HRX27178.1"
                     /translation="CSSSGSAGRRGAVGSTSLGGICHSWSDDGRCISLLKVLFSNDCV
                     FDCAYCVNRRSADVPRATFTPRELADLTIQMYRRNYIEGLFLSSAVVGSPDDTMIQLV
                     RTATILREEHRFGGYIHAKAIPGASASLLGALGLLADRMSVNIELPSETSLRALAPQK
                     SRNSILRPMAFIGGTIEERKKWRRSGRSGPAFVPAGQSTQLMVGASPENDFTILRLSE
                     GLYRSFGLKRVYYSAYIPVADNPLLPARATKPPMLREHRLYQADWLLRFYGFRAEEVL
                     TPEQPDLDVALDPKTGWALGHPDFFPVDVNRASYEEILRVPGIGVRSAQRILTARRGH
                     LLDGDDLGKLGVVMKRARFFVACKSPAFPAFPAGGDLRRLLSSPPRGKEDSRQLRLEF
                     "
BASE COUNT          254 a          425 c          505 g          261 t
ORIGIN      
        1 agaccgccat ctcccttccg ggcatggcgc tgagaaaggc gtagtagccc tcggtgagga
       61 ctcttcgcga cagcctggcg gccagggcgg tgcgtacgga agccgccagt ccctcatccg
      121 tttccgtcgg caccgtttca aaaaggaagg gctgattttt gcccccgccg aggatcccct
      181 ccggccgttc ctttctccgc gaggattcgt ggataaggca gagaaaaccg ttgaaggttc
      241 cgtcaaaggt ccagacagcc atggtcagaa ttccaggcgg agctgccggg aatcctcctt
      301 cccccgggga ggggaggaga gcagtctcct gaggtcgccg ccggcaggaa aagcgggaaa
      361 tgccgggctt ttgcaggcga cgaagaaccg ggcccgcttc atcaccacgc ccagttttcc
      421 caggtcgtcg ccgtcgagga ggtgccccct cctggcggtg agaatccttt gtgcggacct
      481 gacgccgatt ccgggaacgc ggagtatttc ttcgtaggat gccctgttca cgtccaccgg
      541 gaagaagtcc ggatggccca gggcccagcc cgtcttgggg tcgagggcca cgtcgaggtc
      601 aggctgttcc ggggtcagga cttcttcggc ccggaagccg tagaaccgga ggagccagtc
      661 cgcctggtag agtcggtgtt ccctgagcat gggaggcttc gtggcgcggg ccggaaggag
      721 ggggttatcc gccacgggta tgtaggcgga gtaatagacc cgcttgagcc cgaagctccg
      781 gtagagtcct tcggacaggc gcagaatggt aaagtcgttc tccggggagg cccccaccat
      841 gagctgggtg ctctgtcccg cgggaacgaa ggccggtccg gaccggccgg aacgcctcca
      901 tttttttcgt tcctcgatgg ttccgccgat gaaggccatg gggcggagga tggagttcct
      961 gctcttctgg ggggcgaggg cccggaggct tgtctcggaa ggcagctcga tattcacgct
     1021 catcctgtcg gcgaggagcc cgagagcgcc gagaagggac gccgaggcgc cggggatggc
     1081 cttggcgtga atgtagcctc cgaaacggtg ctcctcccgg aggattgtag ccgtccggac
     1141 gagctgtatc atggtgtcgt ccggcgaacc gacgacggcg gagctgagaa agagcccttc
     1201 gatatagttt ctccggtaca tctggatggt caggtccgcg agttcccggg gagtgaaggt
     1261 ggcccgcggg acgtcggcgc tccgcctgtt cacgcagtag gcgcagtcga aaacgcagtc
     1321 gttggagaac aggaccttga gcagggagat gcaccgtccg tcgtcggacc agctatggca
     1381 gatgccgccg agggaggtgc tgcccacggc gccccgcctt ccggcgcttc cgctggacga
     1441 gcagg
//
LOCUS       DAOTHD010000662         1444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_106089_length_1444_cov_2.673146, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000662 DAOTHD010000000
VERSION     DAOTHD010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106089_length_1444_cov_2.673146"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="P5219_10210"
     CDS             complement(<1..959)
                     /locus_tag="P5219_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27179.1"
                     /translation="MKRVLTILAFLLLPLSVWAMTPISDSDLSNVTGQAGVNINVDLL
                     MNVRLGTMAWGDSDGIAAGGWPGWMNGTGTALLSPSDDGGYVGIKDFNLNNLRIKARD
                     TNDGFGEYTTANVGYNTGFMKPLTIDVAQDVTFYGGKTFVRIGLGSLKISMDALTMTV
                     ALQPYDTFAGDLTEEMGTVNVGDITVYISPSSYIDITTPSNTTEGGQGVKLAMNITLD
                     EVTLGYVSWGDSDGLPAGSTGYEDSPTTGATTVVWMAPGAGNAAGYIGLEDINFGIVK
                     INGAVVINVIHSLQGVYSHGGLTPVTVCHIRFQGPLGYFNVDVA"
     gene            complement(1205..>1444)
                     /locus_tag="P5219_10215"
     CDS             complement(1205..>1444)
                     /locus_tag="P5219_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27180.1"
                     /translation="TLRLGTPSMTGPITADFVSFSGRIASGGVAAVEGHRLNLGTRTI
                     TSTDSEFYMQLSTVAVDPGQSPGWWFSWDNATITP"
BASE COUNT          310 a          418 c          408 g          308 t
ORIGIN      
        1 gccacatcca cgttgaagta acccaacgga ccctggaacc ggatatggca cacggttacc
       61 ggtgtgaggc caccatgaga ataaacaccc tggagagagt gtatcacatt gatgacaact
      121 gcaccattga tcttgacgat gccgaagttg atgtcctcaa gaccgatgta acctgccgcg
      181 tttcctgcac caggagccat ccagacgacc gtggtggccc ctgtggtggg gctatcctcg
      241 tagccggtgc tgcccgcggg caggccgtcc gagtcacccc atgacacgta gcccagcgtg
      301 acctcgtcca aggtaatgtt catggcgagc ttcacgccct ggccaccctc ggtagtatta
      361 ctcggtgtgg tgatgtcgat ataggagctc gggctgatgt acacggttat gtcgccgacg
      421 ttcacagtac ccatctcttc ggtcaggtca cctgcgaatg tgtcgtaggg ctgcagggcc
      481 acggtcatgg tcagggcgtc catggagatc ttcagggaac cgagaccgat gcggacaaag
      541 gtcttgccgc cgtaaaaagt gacgtcctgg gcaacgtcga tggtgagggg cttcatgaac
      601 ccggtgttat atccgacgtt ggcagtagta tactctccga agccgtcatt cgtgtccctt
      661 gccttgatcc tgaggttgtt gaggttgaag tccttgatcc ccacataacc gccgtcatcg
      721 gacggggaaa ggagggctgt accggtgcca ttcatccagc caggccaccc gcctgcagca
      781 atgccgtccg agtcgcccca ggccatggtg ccgaggcgga cgttcatgag caggtccacg
      841 ttgatgttca cgccggcctg accggtgaca ttggaaagat cggaatcaga aatgggggtc
      901 atggcccaaa cggaaagggg cagaagcaga aaagccagaa tcgtcaatac tctcttcata
      961 acgttgttgc ctccaatttt tttatttttt taccgttacg ttctctactg cataacctcg
     1021 ctgacattgt aacatctgat catccacgcg cggtctccca ggcatcacct ccttttatcc
     1081 ttactatctg gaaaagcaca gggcagggag aataaccatg gggcgaacgt ctcccgtaca
     1141 tgaaacgtca ttccatgaaa gaacatgcac ctgtgtgcct tcataatccg ttcatactcc
     1201 ccgctcacgg cgtgatcgtg gcgttgtccc acgagaacca ccagcccggc gactggccgg
     1261 gatccaccgc tacggtggag agctgcatgt agaactccga gtcggtgctc gtgatggtcc
     1321 gcgtacccag gttcagccga tggccttcca ccgccgccac accaccgctc gcgatgcggc
     1381 cgctgaagga gacgaaatct gcggtgatgg ggccggtcat ggagggcgtg cccagccgca
     1441 gggt
//
LOCUS       DAOTHD010000663         1444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_106127_length_1444_cov_1.804176, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000663 DAOTHD010000000
VERSION     DAOTHD010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106127_length_1444_cov_1.804176"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            571..>1444
                     /gene="dctP"
                     /locus_tag="P5219_10220"
     CDS             571..>1444
                     /gene="dctP"
                     /locus_tag="P5219_10220"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRX27181.1"
                     /translation="MKHWKLASIGVAAAVLFGVLFIGGIEQASAADVFNWKFQSHHTP
                     GSLAVESVIKPFIERVEKMSGGRLKISLHYAGELVDYAEVDKALQANMIQISNTSSLF
                     FRGAIPMGWVTAPNMPPFVTRSNDEFNELFHHRGLDELVAEALNERGIKYLGCHSVGN
                     TYFWSKKPIKTLEDLKGFKVRFFGSMSDFVEHFGASPVMLPHPETYMAIAMGTLDGSG
                     TAWWLYRDLKLYEVCKYFVGPAFQVPQGMELWASKKAWDELPADLQAIVETAAHAFNT
                     DYMDAVTMQERDMFN"
BASE COUNT          320 a          394 c          417 g          313 t
ORIGIN      
        1 ccctttccgt gaaatgccgt atttcaggct gtccgggagg gcggaaagaa gagccggagc
       61 gaattcccgg cagagcaatg ttccggcggg ccctcgcgga caccggatat accggtccga
      121 tgaccgggga atcgggaaca tccgcccggc ttgacttcgt aattgagagt ttacactact
      181 gacgaaacag agaaaacggc gaaataattc agcttcctgg caatccgaga aaagtttctt
      241 tggaagtcgt atccagttat gcactctctt gaatacaatc cgggaggtcg gcggaaaaaa
      301 ggaagaaacg ccaatttgtc tgatgtcctc tgatgaaggg cttccggcac ggcggttcgt
      361 tatccccgga gctgttttcc gtgtctggac caggcggttt gttctttagc ggggcaggct
      421 tttcccgctg aaaaggcatc tatcttttca ggcgtccggc cgacggtctc cggagcgttt
      481 ccgtcaggtc cgtccgctca ggcagggagg tgatatgttc tccgaaaagg acgatggggc
      541 cggatcagaa aatctgaagg aggtgctttg atgaaacatt ggaaactggc ttcgattgga
      601 gttgcagcgg cggtactttt cggcgttctg tttatcggcg gcattgagca ggcctcggcg
      661 gcggatgttt tcaactggaa gttccagagc caccacaccc cggggtccct cgcggttgaa
      721 tccgtcatca agccttttat tgaaagagtg gagaagatgt ccggcggcag gctgaaaatc
      781 agccttcact atgccggtga actggtggac tacgctgaag tggacaaagc cctccaggcg
      841 aatatgatcc agatctccaa cacgagttcc ctgttcttca ggggagccat ccccatggga
      901 tgggtcaccg cgcccaacat gcctcccttt gtcacccggt ccaacgacga gttcaacgaa
      961 ctcttccatc acagggggct cgacgagctt gtggctgagg ctctgaacga gcggggcatc
     1021 aagtacctcg gatgccacag cgtgggcaac acgtatttct ggagcaagaa accgattaaa
     1081 acccttgaag acctgaaggg attcaaagtc cgtttcttcg gctccatgtc cgatttcgtg
     1141 gagcacttcg gtgcctcccc cgtcatgctt ccccacccgg aaacctacat ggccatcgcc
     1201 atgggcaccc tcgacggcag cggaacagcc tggtggctgt accgcgacct gaagctctat
     1261 gaagtctgca agtacttcgt cgggccggcc ttccaggttc cccagggaat ggaactctgg
     1321 gcttccaaga aggcgtggga cgagcttcct gcggatctcc aggccatcgt cgagacggcg
     1381 gcccacgcgt tcaacaccga ttacatggac gcagtgacca tgcaggaacg ggatatgttc
     1441 aaca
//
LOCUS       DAOTHD010000664         1443 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_106166_length_1443_cov_3.289625, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000664 DAOTHD010000000
VERSION     DAOTHD010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106166_length_1443_cov_3.289625"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="P5219_10225"
     CDS             complement(<1..197)
                     /locus_tag="P5219_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27182.1"
                     /translation="MLEDVPQQQGYGEKEQLGTYLPRRQGTGMSNDVFLLEVLSPISG
                     RQNKQMIPYETVLVNRRIMPQ"
     gene            210..>1443
                     /locus_tag="P5219_10230"
     CDS             210..>1443
                     /locus_tag="P5219_10230"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRX27183.1"
                     /translation="MAAIIVGRYVGEAAFAAIGVGGTVMNLFIFVLTGCCTGVSIILA
                     SLFGKGDLHNLRKECFLAVVFGAGFTLFVSLASIALLPSLLEAIHTPAEIFEGTRQYL
                     NIILGGLLATFFYNLSAASLRAVGNTRYALVFLVTAVMLNAALTFVLVALFGCGIAGA
                     AWATVFSQACSALFCMAYIRKKLPFLVPTKEDMRYDGALLKKTILFASLSALHQSSLY
                     IGKLLVQGAVNLLGTAAIAAYTATGRIEGFSLAVGESGAESISVFVAQNTGAGNHTRA
                     LKGFLEGLASLALLSISVSLLLFVTARPGVILFVGANQGEAVAEGVAYMRALCFFYIF
                     SYIGSSFVGFYRGTGRINIPFIGTTLQISVRVVLSYWLAPLLGLKAVGLATGIGWISI
                     VTFQLLVFSSIRRRERREE"
BASE COUNT          232 a          441 c          425 g          345 t
ORIGIN      
        1 tcctgcggca tgattctacg gttcactaat actgtttcgt agggtatcat ctgtttgttt
       61 tgtctgccgg atattggtga aagtacttcg aggaggaaca catcattact cattcccgtt
      121 ccctgacgtc ggggaagata ggttcccagc tgctctttct cgccataccc ctgctgctgg
      181 ggaacatctt ccagcaactg tacaatttcg tggcagccat catcgtcggc cggtacgtag
      241 gagaggcggc cttcgccgcc ataggcgtcg gcgggacggt gatgaacctg ttcatctttg
      301 tcctcaccgg ctgctgcacc ggcgtgtcca tcatcctcgc ctccctgttc ggaaaagggg
      361 atttgcacaa tctgcggaag gagtgcttcc tggccgtggt cttcggggca gggttcaccc
      421 ttttcgtgag tctcgcctcc atcgcccttc tcccctccct gctcgaggcg attcacaccc
      481 cggcggagat attcgagggc accaggcagt acctgaacat catcctggga gggctcctgg
      541 cgaccttttt ctacaatctc tccgccgcct cgctcagggc cgtcggaaat acccggtacg
      601 ccctggtttt cctggtgacc gccgtgatgc tcaacgcggc tctgaccttc gtgcttgtgg
      661 ccctgttcgg ctgcgggatc gcgggggccg cgtgggcgac ggtcttttcc caggcctgct
      721 ccgccctgtt ctgcatggcg tacatcagga agaagcttcc cttccttgtg ccgacgaagg
      781 aggatatgcg gtacgacggg gccctgctga aaaagacgat ccttttcgcc tccctttcgg
      841 ctctgcacca gtcgagcctg tacataggca agctgctggt gcagggggcc gtgaacctgc
      901 tgggcacggc ggccatcgca gcctacaccg ccacgggaag gatcgaggga ttctccctcg
      961 ctgtgggtga aagcggggct gaatccatct ccgtcttcgt tgcccagaat accggggcgg
     1021 gaaatcatac aagggccctg aaggggttcc tggaggggct cgcctccctg gccctcctca
     1081 gcatttccgt ctcgctgctg ctgtttgtca ccgcccgccc gggcgtgatc ctgttcgtgg
     1141 gggcaaacca gggagaagcg gtcgccgagg gagtggccta catgcgggcc ctctgtttct
     1201 tctacatatt ctcctacatc ggcagttcct ttgtgggatt ctacaggggc accggccgga
     1261 tcaacattcc cttcatcggc acgaccctcc agatttctgt tcgggtggtc ctttcgtact
     1321 ggctcgctcc cctgctggga ctgaaagccg tggggctggc caccgggata ggatggatct
     1381 ccatcgtgac cttccagctc ctggtttttt ccagcatacg gagaagggag cggcgggaag
     1441 agg
//
LOCUS       DAOTHD010000665         1443 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_106190_length_1443_cov_2.753602, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000665 DAOTHD010000000
VERSION     DAOTHD010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106190_length_1443_cov_2.753602"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="P5219_10235"
     CDS             complement(<1..405)
                     /locus_tag="P5219_10235"
                     /inference="COORDINATES: protein motif:HMM:NF017078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HRX27184.1"
                     /translation="MSLRKKVFLYILVTILFLILLMSLTSNFLAMKAFEGVERRFFEQ
                     DMARVRSRLAEELHIVGRYAFDWGAWDSMYLFMERKEPSRFQEMLDPMSLRALGLDIL
                     AVVDTDLSPVVAYSVTETGGEKKLSPETIAGLK"
     gene            complement(547..1155)
                     /locus_tag="P5219_10240"
     CDS             complement(547..1155)
                     /locus_tag="P5219_10240"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRX27185.1"
                     /translation="MTVPGEVLLFFRGAILGFSIAAPVGPIGILCIRRTLDGGFRSGF
                     LSGLGAATADGLYGLAAALGLAALSSFLLEWNGPLRTGGGLFLLYLGLRALLDRSAPP
                     AEGQPARTAPGTTDYFSTLFLTLTNPMTILSFSAIFTGLGPILSPWTLVAGVFSGSAA
                     WWLILCGTADRLGTMISPAVLLWIRRGSGILLASFGLSVLLF"
     gene            complement(1152..>1443)
                     /locus_tag="P5219_10245"
     CDS             complement(1152..>1443)
                     /locus_tag="P5219_10245"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HRX27186.1"
                     /translation="AAAGFGLTAFVEVTLGHPSSREAFLDLAASLPEIRECHHVTGSS
                     DYLLKVACSGTAHLEELISERLKGCPGVTATRTSVVLSTPRDRPESPLPDLP"
BASE COUNT          347 a          400 c          467 g          229 t
ORIGIN      
        1 cttcaaaccg gcgatggttt ccggggaaag ctttttctct ccccccgtct cggtgacgga
       61 gtaggccacg accggtgaga ggtccgtatc gaccacggcg aggatgtcga gaccgagagc
      121 ccggaggctc atggggtcga gcatttcctg gaagcgggag ggttcctttc tctccataaa
      181 caagtacatg ctgtcccagg ccccccagtc gaaggcatat ctgccgacga tgtggagctc
      241 ttcggccagc ctgctgcgca cgcgggccat atcctgctcg aaaaaccgcc gctccacgcc
      301 ttcaaaagcc ttcatggcca aaaagttgga ggtgaggctc atcagcagga tcaggaacag
      361 aatggtgacc aggatgtaca gaaacacctt tttccgcaag gacatggcgg cctctcaaaa
      421 gtaaatttat cgccaattta aaagtagtat accttgaaaa agagtgaggg aaagactttc
      481 gtccattttg ttcactggaa aaaagaggaa aatgcaaaaa accctttccg gatcacctgg
      541 ggtgtatcag aaaaggagaa cggacaggcc gaaggaagcg agaagaatgc cggatcccct
      601 gcggatccac agcaggactg ccggagagat catggtcccc agacggtccg cggttccgca
      661 gaggatgagc caccatgcgg ccgagccgga aaacaccccg gccacaagcg tccaggggga
      721 cagtatgggc ccgaggcccg tgaagatggc agagaagctc aggatggtca tggggtttgt
      781 gagggtgagg aaaagagtgg aaaaatagtc tgtcgtcccc ggagcagtcc gggcaggctg
      841 tccttccgcg gggggagcgg agcggtcgag gagagcccga aggccgagat agagcaggaa
      901 gagcccccct ccggtccgga gaggtccgtt ccactccagg agaaaggacg acagggcggc
      961 cagcccgaga gccgctgcca gaccgtaaag accgtctgcg gtcgccgcgc ccagacccga
     1021 gagaaatccg gagcggaatc ctccatcgag ggtgcgccgg atgcacagga tgccgatggg
     1081 gcctacggga gccgcgatgg aaaaacccag tatcgctccc cggaagaaca gcaggacctc
     1141 gcccggaaca gtcatggcag gtcaggaagg ggagattcgg ggcggtccct gggagtggac
     1201 aggaccacgg atgtcctggt ggccgtcacc ccgggacatc ccttcagcct ttcggagatc
     1261 agctcctcca ggtgagcggt gcctgaacag gcgaccttca gcaggtaatc ggagctgccc
     1321 gttacgtggt ggcattcccg aatttcagga agggatgccg ccaggtccag gaacgcctcc
     1381 cgggaagaag gatgtccgag ggtgacttcc acgaaggccg tcagcccgaa tccggcggcg
     1441 gcg
//
LOCUS       DAOTHD010000666         1443 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_106216_length_1443_cov_2.346542, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000666 DAOTHD010000000
VERSION     DAOTHD010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106216_length_1443_cov_2.346542"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="P5219_10250"
     CDS             complement(<1..372)
                     /locus_tag="P5219_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27187.1"
                     /translation="MKELTEILNKKPPMSKADQKTWRKLTDELHTISKSNGETLRNIA
                     EKAPEIGGKITKQQVRTNPDGTVTDLKTGKTMTRSQFADRTAPGTKKTVPKVIKPELP
                     KKPAVPTPPPPSKLVKVGTGII"
     gene            complement(369..1367)
                     /locus_tag="P5219_10255"
     CDS             complement(369..1367)
                     /locus_tag="P5219_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27188.1"
                     /translation="MKTAGTRHYGKFFSLFFLFTLLALHLFSFGGQALGASLEEEMLS
                     ELGKVRDSFAASEPQRGQFSDEGSYSSARKKWLNDFQKASDGVRTTYKNRDGRFTEIK
                     DAIKSAGMEKEFLNSGSAPKTPSSDIDLTEMKPGSAKKLANQLNSKGYSIRPDPNVPG
                     RYVDPAKKLVIWETPPNLRTGSPEWRNWVTSRAGAEDTFSTAGGLFETSGGKVGAKDP
                     AGAVLDNVKKAMEAGIGKNPAGGEIDSKTVGKSVKKAMEWTGTTPKKGEAAEFARQAE
                     NLRKGRSWEEAGITSPNDPPEVKNKKIRQWLDKPRTPYRSPTRRPRGRARRSRTEK"
BASE COUNT          201 a          434 c          392 g          416 t
ORIGIN      
        1 aataatgccc gttccgacct tcaccagctt cgacggggga gggggcgtcg gaaccgccgg
       61 ttttttggga agctcgggct tgatcacctt cgggactgtt ttttttgttc ccggcgctgt
      121 cctgtccgca aactggctcc tggtcatagt cttgcccgtt ttcaggtccg ttaccgtgcc
      181 gtcggggttt gtccgcacct gctgtttcgt gatctttccc ccgatttccg gggccttttc
      241 cgcaatgttt ctcagggtct caccgttgct tttcgagatt gtatggagct cgtccgtcag
      301 ttttctccac gtcttctggt ccgctttgga catggggggc tttttgttca gtatttccgt
      361 gagttccttc acttttccgt ccgtgatctt ctggctctgc ctctgggcct tctggtagga
      421 cttcgatagg gagtcctggg cttatcgagc cactgccgga tctttttgtt tttcacctcc
      481 gggggatcgt tggggcttgt tatacccgct tcctcccagc ttcttccttt ccgcaggttc
      541 tccgcctgcc tggcgaattc ggccgcttca ccctttttcg gagtcgtgcc cgtccattcc
      601 atggctttct tgacgctttt gccgacggtc ttggaatcta tctccccgcc tgcggggttc
      661 tttccgatcc ctgcctccat cgcttttttg acgttgtcga ggaccgctcc ggcggggtct
      721 ttcgcgccca ccttgccgcc tgaggtctca aagagaccgc ccgcggtgga aaaggtatct
      781 tccgcgcctg ccctggaggt cacccagttc cgccattccg ggctcccggt ccgcaggttc
      841 gggggggtct cccagatcac gagcttcttt gccggatcca cgtaccgtcc gggcacgttc
      901 gggtccggtc tgatggagta gcccttcgag ttgagctggt tcgccagctt tttcgcgctc
      961 ccgggcttca tttccgtaag gtcgatgtcg ctcgacgggg ttttcggagc gcttcctgag
     1021 ttgaggaatt ccttttccat gcctgcggac ttgatggcgt ccttgatctc ggtgaaccgg
     1081 ccgtcccggt ttttgtaggt tgtccgaacg ccgtcggaag ccttctggaa atcattcagc
     1141 cattttttcc gtgcggagct gtaacttcct tcatcggaaa actgacccct ttggggttca
     1201 gaggccgcaa aactatcccg taccttgccc agttcgctca gcatctcctc ctcgagggat
     1261 gccccgagcg cctgtccgcc gaaggagaag agatgaaggg cgagcagggt gaacaggaaa
     1321 aagagagaga agaatttccc gtaatgcctc gttcccgctg tcttcatttc cttcacttcc
     1381 cttcggtcca ttcagcaaga actgaacgcg cataggaagc cttttccgtg tcctgggaaa
     1441 acg
//
LOCUS       DAOTHD010000667         1442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_106286_length_1442_cov_3.284066, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000667 DAOTHD010000000
VERSION     DAOTHD010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106286_length_1442_cov_3.284066"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="P5219_10260"
     CDS             <1..766
                     /locus_tag="P5219_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRX27189.1"
                     /translation="APVVVGFIFGGDMLGGLLPKKTKRRRMKVSEARTMLAAEEAEKL
                     VDMDAVVREALDKAQEEGIVFLDEIDKVVARGGTGGPDVSREGVQRDLLPIVEGSPVQ
                     TKYGTVNTDHILFITAGAFSSVKPSDLVPELQGRFPIRVELQPLTRDDLARILVEPEN
                     SLLRQYTALLATEGVEIDFTPEAVAEMARLAERMNGEMENIGARRLHAMIEHLLEDIS
                     FGAPEEGQGPVPVTPEFVRERLEPLISDSDIRRYLL"
     gene            828..>1442
                     /locus_tag="P5219_10265"
     CDS             828..>1442
                     /locus_tag="P5219_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HRX27190.1"
                     /translation="MAAKKIKASEEIKATEPHVIDEDPGLKDLLEKTRQVGRALQSRR
                     EGTRPDYNKLAKLLCEFSTANVYLINREGKILGHSWISEYHSEEVSNFIDKGYMPEIF
                     VEKMNQHRETMLSETDGYLFDDASAEAPEKHMLYIPIYGAAERLGTLLLVRFFQPFSM
                     RDLVLAEYLATLVGIEILHERTKNIEERSRERLIVQMAMRALSYS"
BASE COUNT          324 a          414 c          456 g          248 t
ORIGIN      
        1 cgcgccggtg gtggtcggtt tcatcttcgg aggggacatg ctcggcggcc tgctgcccaa
       61 aaagacgaag cgccgcagga tgaaggtgtc ggaggcccgg accatgcttg ccgccgagga
      121 ggcggagaag cttgtggaca tggacgccgt cgtccgggag gccctcgaca aggcccagga
      181 agaaggaatc gtctttctcg acgagatcga caaggttgtt gcccggggcg gcacgggcgg
      241 tcccgacgtg agccgggagg gcgtgcagcg ggaccttctg cccatcgttg aaggctcccc
      301 cgtccagacg aagtacggca cggtaaacac ggaccacatc ctgttcatca ccgcaggggc
      361 cttttcgagc gtgaagcctt cggacctggt gcctgaactg cagggcaggt ttcccatccg
      421 ggtggagctt cagcccctta cgagggatga cctggcccgg attctcgtgg agccggagaa
      481 cagcctgctg aggcagtata cggccctcct cgccacggaa ggcgtcgaga ttgacttcac
      541 cccggaggcg gtggccgaaa tggcccgcct ggcggagcgg atgaacggtg aaatggaaaa
      601 catcggggcc aggaggctcc atgcgatgat agagcacctt ctcgaagaca tcagtttcgg
      661 cgcccccgag gaggggcagg gccccgttcc cgtgacgccc gaattcgtgc gggagagact
      721 ggagcccctt atttcggaca gcgatatcag aagatatctt ctgtagatca cattacataa
      781 tccggtacgg acagtacgaa cggataccat atcaggaggg attttgagtg gctgcaaaga
      841 aaatcaaggc aagcgaagaa atcaaggcga cggaaccgca cgtgatcgat gaagatccgg
      901 gactgaagga tctcctcgag aagacccggc aggtgggcag ggccctccag agccgcaggg
      961 agggaaccag acccgactac aacaagctcg ccaagctgct gtgcgagttt tccacggcca
     1021 acgtgtacct gatcaacagg gagggcaaga tcctgggtca ttcctggata tcggagtatc
     1081 attccgagga agtctccaac ttcatcgaca agggatacat gcccgagatc ttcgttgaga
     1141 agatgaacca gcaccgggag accatgctga gcgagaccga cgggtacctg ttcgacgacg
     1201 cgagcgccga ggctcccgag aagcacatgc tctacatacc catttacggg gccgccgaga
     1261 gactcggcac gctgcttctc gtccggttct tccagccctt ctccatgcgg gacctcgtcc
     1321 tggcggagta cctggccacc ctcgtgggca tcgaaattct ccacgagagg acaaagaaca
     1381 tcgaggaacg ctccagagag cggctcatcg tccagatggc catgagggct ctttcctact
     1441 cc
//
LOCUS       DAOTHD010000668         1442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_106307_length_1442_cov_2.788032, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000668 DAOTHD010000000
VERSION     DAOTHD010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106307_length_1442_cov_2.788032"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1442
                     /gene="nhaC"
                     /locus_tag="P5219_10270"
     CDS             <1..>1442
                     /gene="nhaC"
                     /locus_tag="P5219_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HRX27191.1"
                     /translation="ERGRKPSFGEALFVLVVDALIISYGVLDIHFESGATFGLALSAH
                     VPLFIAAVFTAIVGVFFIGKKWRDVEEGMINGITVALQAVLILMTIGGLIGAWIQSGV
                     VPTLIYYGLNLLSPQYFLLASLLICSIVSLSTGSSWSTSGTVGIALMGIGAGLGVPAP
                     VTAGFVISGAYFGDKMSPLSDTTNLAPAVAGSDLFSHIRAMLWTTGPTLLIVAGIAVY
                     MGGSYSGAELDSSRISVIQQMMSSEFAISKIGFLPPLLVLLLAALKVPALPGITAGML
                     LAAGMSLFQGVGLGDVLGALHSGYTPGLISQVAESADIGAVNELLKGAVVFQPENFET
                     VKEVASMLNDLLARGGLTGMLETVALILVALVLGGIMETCGFLEVLLERLMAKVKSVF
                     GLVGAVISSCVFTNMFLGDQYLALVMPGRMFRPAFEDFRKDGKGLDPKLLSRSLEDSG
                     TLTSVLVPWNTCGAYNSGVLGVPTLSYLPY"
BASE COUNT          260 a          435 c          398 g          349 t
ORIGIN      
        1 tgaaagggga agaaaaccca gcttcggcga agccctcttt gttcttgttg tcgatgccct
       61 tatcatcagc tacggagttc tggacatcca tttcgagtca ggcgccactt tcggactggc
      121 tctgtcggct catgtgcctc ttttcatagc ggcagtcttc accgccattg tcggagtgtt
      181 ttttatcggc aaaaagtggc gcgatgttga agaaggcatg atcaacggca ttactgttgc
      241 cctccaggcc gtcctgatcc tcatgaccat cggaggcctc atcggggcct ggatccagag
      301 cggagtcgtt cccaccctca tctattacgg cctgaacctc ctttctcccc agtatttcct
      361 cctggcgtcg cttctcatct gctcgatcgt gtccctttcc acgggcagct catggagcac
      421 aagcggcacc gtgggcatcg ccctcatggg aataggtgct gggctcggcg tccctgcccc
      481 agtaaccgca ggattcgtca tctccggggc ctatttcggc gacaagatgt cacccctgtc
      541 tgataccacg aatcttgccc ccgcagtggc cggaagcgac cttttcagcc acatcagggc
      601 catgctgtgg acaaccggcc ccactcttct gatagtggcc ggaatagccg tgtatatggg
      661 cggaagctac agcggagccg agctcgatag ttcccgcata tcggtcatcc agcagatgat
      721 gtcgtcagaa tttgccattt ccaagatcgg attcctgccg ccccttctcg ttcttcttct
      781 tgcggcgctg aaagtccccg ccctgccggg gataaccgca ggaatgctgc ttgccgcagg
      841 catgtccctc tttcagggag taggactggg cgatgtgctg ggagctcttc attccggcta
      901 taccccgggc cttatcagcc aggttgccga atccgccgac ataggagcgg tgaatgaact
      961 cctcaagggg gcggtcgttt tccagccgga gaacttcgag accgtaaagg aagtggcctc
     1021 catgctgaac gaccttcttg ccaggggcgg cctgacgggc atgctcgaaa cggtggccct
     1081 catcctggta gcccttgtgc tgggcggcat catggagacc tgcggcttcc tcgaggtgct
     1141 ccttgaaagg ctcatggcga aggtgaagtc cgtgttcggt ctcgtggggg cggttatttc
     1201 ctcctgcgtt ttcaccaaca tgttcctcgg ggaccagtat cttgccctgg tcatgcctgg
     1261 gaggatgttc cggcctgcct ttgaagattt ccgaaaagac ggcaaggggc tggatccgaa
     1321 acttctttcc cgatcccttg aggattccgg aacgctcaca tccgtcctcg tcccctggaa
     1381 tacatgcgga gcgtacaaca gcggcgttct tggcgtaccg accctgtcat accttcccta
     1441 tg
//
LOCUS       DAOTHD010000669         1441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_106397_length_1441_cov_3.611833, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000669 DAOTHD010000000
VERSION     DAOTHD010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106397_length_1441_cov_3.611833"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="P5219_10275"
     CDS             <1..393
                     /locus_tag="P5219_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27192.1"
                     /translation="NIKTNNVSEVGYRLILDHDCSRWEFIFRDDQIGEDDWMTLRFMV
                     KAFPETPLAFGDKRLSNPFPNQGEFKGKTPEGVPSRRPVMEDDWGDDGLTYKSDPTQL
                     KETGENPVPSTEADSEEPSGEDGNLLNP"
     gene            complement(361..822)
                     /gene="greA"
                     /locus_tag="P5219_10280"
     CDS             complement(361..822)
                     /gene="greA"
                     /locus_tag="P5219_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRX27193.1"
                     /translation="MTQKGYEKLMAELVELRSTGRAEISRQLEEARSFGDLSENAEYA
                     AAKEEQAKLETRISYLEFQLSKAKILDSSTIDNSSVTLGTTVTIQDINNKAEFSYTIV
                     GSEESDPKASKISSSSPVGQALLNKKVGDEVHVKVPRGMRHLRIKEISVLA"
     gene            1008..>1441
                     /locus_tag="P5219_10285"
     CDS             1008..>1441
                     /locus_tag="P5219_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRX27194.1"
                     /translation="MARDSRSRKKKTSAAKKDGKLRYIPLGGLGEVGKNMYALEYEDS
                     ILVVDCGLMFPDDEMFGIDFVIPDITYLEERKDKIVGIVITHGHEDHIGGLPFNLPKI
                     PAPLYGTKLTLGMVGNKLTEWAPAFSPDYREIRAGDRVEMGP"
BASE COUNT          306 a          403 c          422 g          310 t
ORIGIN      
        1 aacatcaaaa ccaataacgt gagcgaagtc ggctaccggc tgattctcga ccacgactgc
       61 agccggtggg aatttatttt ccgggatgac cagatcgggg aggatgactg gatgaccctc
      121 cggttcatgg tgaaagcctt cccggagacg cccctggcct tcggcgacaa aaggctctcg
      181 aaccccttcc ccaaccaggg cgagttcaag gggaaaacgc ctgaaggcgt tccgtcccgc
      241 aggcctgtca tggaagatga ctggggcgac gacggcctga cctacaagtc cgaccccacg
      301 cagttgaaag agacagggga gaaccctgtc ccttcaaccg aagcagattc ggaggagccc
      361 tcaggcgagg acggaaatct ccttaatccg taggtggcgc attcccctgg gtaccttgac
      421 gtgcacttcg tcgcctacct tcttgttcag aagggcctgc ccgaccgggc tggacgagga
      481 aatcttgctc gccttcgggt cggattcttc ggaacctacg atggtatatg agaattcggc
      541 tttgttgttg atgtcctgga tggtgaccgt ggttccgagg gtgacgctgc tgttgtcgat
      601 ggtggaggag tcgaggatct tggccttgct gagctggaat tcgaggtagg atatgcgcgt
      661 ctcgagtttt gcctgctctt ccttcgccgc ggcgtattcg gcgttctcgc tgaggtcccc
      721 gaaggacctg gcctcttcga gctggcggga tatttccgct cgtcccgtgc ttcggagctc
      781 aacgagctcc gccatgagct tctcgtatcc cttctgtgtc atggtggagg ggctgtcgct
      841 ggtgtgattg ctcaaacttt ctacctcccg gtaacgtcct ctgagtgagg caaggttcat
      901 attacgtttg atatggaatt ctaacaaaga atgttctgtt cggcaatacg gaaggagaga
      961 ttcctccttc ccgccggaac tgaatcggag aaaggcaagg tgtaccaatg gcgagagatt
     1021 cacgaagccg caagaagaaa acctctgcag cgaagaagga cggcaagctg cggtatatcc
     1081 ccctgggcgg cctgggggaa gtgggcaaga acatgtacgc cctcgagtac gaggactcca
     1141 tcctcgtcgt agactgcggt ctcatgttcc ccgacgacga gatgttcggc atcgacttcg
     1201 tcatcccgga tatcacctat cttgaagagc gaaaagacaa gattgtcggc atcgtcatca
     1261 cccacggcca cgaggaccac atagggggcc ttcccttcaa tctcccgaaa attcccgccc
     1321 ccctctacgg cacgaagctc accctgggca tggtggggaa caagctcacg gaatgggccc
     1381 ccgccttttc acccgactac cgggaaatac gggccggaga ccgggtggaa atggggccct
     1441 t
//
LOCUS       DAOTHD010000670         1440 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_106537_length_1440_cov_2.259928, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000670 DAOTHD010000000
VERSION     DAOTHD010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106537_length_1440_cov_2.259928"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="P5219_10290"
     CDS             complement(<1..611)
                     /locus_tag="P5219_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRX27195.1"
                     /translation="MDPMAFDLPGIDLSWCPGCGDFKILDSIKKALAELGLGPLDVVM
                     VSGIGQAAKTPHYMKAHFFNGLHGRALSNATAIKAANPALEVIAVGGDGDMYGEGGNH
                     FTHTIRRNPGITNLVFNNMVYGLTKGQASPTSPRGFRTSVQVDGVHAQPFNPLAAALV
                     QGATFVARAFAGNGDETKEIIKRAVRHKGYALVDIFQPCVSFNS"
     gene            complement(611..>1440)
                     /locus_tag="P5219_10295"
     CDS             complement(611..>1440)
                     /locus_tag="P5219_10295"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRX27196.1"
                     /translation="SPRPGPATGMATRTAQADLDLALYSGHGEFPRALYAPGTMESAF
                     RLTQEAFEIALKYQTPAIVLTDQYFMNAFYNLDADGLEWLDEPPSVRETEEDYRRYED
                     TPDGCSPFGVPGWGKGIVGADSHEHDEAGHVYEDFDLRTRMQDKRMRKLAGMTDRALP
                     PVLVGPESFSNLVICWGSTLPIVREALGRLGRDDTALLAFEQVWPLAPSAGDLLRKAS
                     FTAVVEGNSTGQFAGLLRKTFGVAADRTVLKYNGLQFSVEEVTDRLGEILPGKGGGR"
BASE COUNT          252 a          441 c          467 g          280 t
ORIGIN      
        1 gagttgaagg agacgcaggg ctggaagata tccaccaggg cgtatccctt gtggcggacg
       61 gccctcttga tgatctcctt tgtctcgtcg ccgtttccgg caaaggcccg ggccacgaaa
      121 gtcgctccct ggaccagggc ggcggcaagg ggattgaagg gctgggcatg gaccccgtcc
      181 acctgaacgg aggtccggaa tcccctgggg ctcgtgggcg aggcctgccc cttggtgagg
      241 ccgtagacca tgttgttgaa caccaggttg gtgattcccg ggttccgacg gatggtatgg
      301 gtgaaatggt ttcctccctc gccgtacatg tcgccgtccc ctcccacggc gataacctcc
      361 agggcggggt tcgccgcctt gatggccgtt gcgttggaga gggcccgtcc gtggaggccg
      421 ttgaagaaat gggccttcat gtagtggggg gtctttgccg cctggccgat ccccgacacc
      481 atgaccacgt cgaggggtcc gaggccgagc tcggcaaggg ccttcttgat ggagtcaaga
      541 attttaaagt ccccgcagcc ggggcaccac gaaaggtcaa tgccgggaag atcaaacgcc
      601 atggggtcca tcatctgccg cctcctttgc cgggaagaat ttcgccgagc ctgtcagtga
      661 cctcttccac agagaactgc aatccgttgt atttcagcac tgtgcggtcc gccgccacgc
      721 cgaaggtttt tcgaagcagc ccggcgaact ggccggtgct gttcccctcc accacggcgg
      781 tgaaggatgc cttccgcaga aggtccccgg ccgagggcgc gaggggccat acctgctcga
      841 aggccagcag ggccgtatcg tcccgcccga ggcgtccgag ggcttccctg acgataggga
      901 gagtggaacc ccagcatatc accaggttcg agaaggattc ggggcccacc agcaccgggg
      961 gaagtgcccg gtccgtcatt ccggcgagct tccgcatccg cttgtcctgc atcctggtcc
     1021 ggaggtcgaa gtcctcgtag acatgtcccg cctcgtcgtg ttcgtggctg tcagctccca
     1081 cgatgccctt tccccagccg ggaacgccga agggtgaaca gccgtcggga gtatcctcgt
     1141 agcggcggta gtcttcttcg gtttccctga cggacggcgg ttcatccagc cactcgaggc
     1201 cgtccgcatc caggttatag aaggcgttca tgaaatactg gtccgtcagc acgatggccg
     1261 gcgtctggta cttcagggcg atttcaaagg cttcctgggt cagcctgaag gcactctcca
     1321 tggttcccgg agcgtagagg gcccggggaa attcaccgtg tcctgaataa agggcaagat
     1381 ccaggtcggc ctgcgccgtc cgggtggcca tccccgtggc agggcccggt ctcggagaaa
//
LOCUS       DAOTHD010000671         1434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_107180_length_1434_cov_2.773749, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000671 DAOTHD010000000
VERSION     DAOTHD010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107180_length_1434_cov_2.773749"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..>1434
                     /locus_tag="P5219_10300"
     CDS             236..>1434
                     /locus_tag="P5219_10300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D C-terminal
                     domain-containing protein"
                     /protein_id="HRX27197.1"
                     /translation="MRILHTSDWHLGRTLYGRKRYEEYEAFLEWLAQAIRDNGIDVLV
                     VAGDVFDTTAPGNRAQELYYRFLCRAASLCCRHVVVISGNHDSPSFLDAPKELLKALE
                     VHVTGSPSGNPEDEVLVLRNGQGVPELIVCAVPHLRDRDIRTAEAGESIEDKERKLLD
                     GIRDHYASVAACAEGKRRELGGAIPLVATGHLFAAGGRTVEGDGVRELYVGSLAHVDA
                     GIFPPCFDYVALGHLHVPQKVSGSEMVRYSGSPLPMGFGEAKQQKSVCVAEFGGRETS
                     VRLVPVPVFQQLEQIRGGWDRISGRIFELASQGSRAWLEVVYDGNEILGDLRERLEEA
                     VGNTAMEVLRVKNTGAVERALAQTLEEETLDDLDEDDVFQRCLEANRVPEDQHAELFA
                     VYREAVLS"
BASE COUNT          262 a          449 c          490 g          233 t
ORIGIN      
        1 gcaccggcgg cccaagcgag caccggtgac gctccccgct gcctgcggat ttaggggagt
       61 caccgggaat tgacggcccg gcggaatagg ccgacaatgg ggggaacctt accggaggtg
      121 cccccatgaa aagaaagaaa tcctcgccct acagtctctt ccggaccccg gctggatccc
      181 tttgccccgg ggatacacct tttcgagccc gcgaaggggc tctcacacgc cggaaatgag
      241 aatcctccac acgtcggact ggcatctcgg ccgcaccctc tacggccgaa aacggtacga
      301 ggaatacgaa gcctttctgg agtggctggc ccaggcgatc cgggacaacg ggatcgacgt
      361 cctggtggtg gcgggcgacg ttttcgacac cactgcgccg ggcaaccggg cgcaggagct
      421 gtattaccgc ttcctctgcc gggcggcttc cctctgttgc cgccacgtgg tggtgatctc
      481 cggaaaccat gattccccct ccttcctcga cgccccgaag gaactgctga aggccctgga
      541 ggtccatgtc accggcagcc cgtccggaaa cccggaggac gaggtgctgg tgctccggaa
      601 cggccaggga gtccccgagc tgatcgtctg tgccgtgccc catctccggg accgggatat
      661 ccgcacggcc gaggcgggcg agagcatcga agacaaggag cgcaagctcc tggacggcat
      721 ccgggatcat tacgcctcag tcgccgcctg tgccgaagga aagcggaggg agctcggggg
      781 cgccatcccc ttggtcgcaa cgggccatct gttcgccgca ggaggccgga cagtggaagg
      841 cgacggggtc cgggagcttt acgtaggctc tctggcccac gtggatgccg gaatcttccc
      901 gccctgtttt gactatgtgg cgctgggtca cctccacgtt ccccagaaag tgagcggctc
      961 tgaaatggtc cgttacagcg gatcgcccct gcccatgggc ttcggcgagg cgaagcagca
     1021 gaagagcgtc tgcgtggcgg agttcggcgg aagggagacc tcagtccgcc tggtcccagt
     1081 tccggtgttc cagcagctcg agcagatcag ggggggctgg gaccgcatct ccggacggat
     1141 cttcgagctg gcctcccagg ggtcccgggc atggctcgaa gtggtctacg acgggaacga
     1201 aatcctgggt gacctccggg agcggctgga agaggcagtg gggaataccg ccatggaggt
     1261 gctccgggtg aagaacaccg gggccgtcga gcgggcgctg gcgcagaccc tggaggaaga
     1321 aaccctggac gacctggacg aggacgacgt cttccagcgg tgtctcgagg cgaaccgggt
     1381 tcccgaagac cagcatgccg agctgttcgc ggtctaccgg gaagcggtcc tctc
//
LOCUS       DAOTHD010000672         1433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_107270_length_1433_cov_2.787373, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000672 DAOTHD010000000
VERSION     DAOTHD010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107270_length_1433_cov_2.787373"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="P5219_10305"
     CDS             <1..249
                     /locus_tag="P5219_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HRX27198.1"
                     /translation="SVQDAERLGIAHGEEIRVASRRGEVRIKANVSKRIAPGMVWMSF
                     HFWETNANHLLSGDLKHFDPDTMTPSFKACAVRIEKIA"
     gene            249..848
                     /locus_tag="P5219_10310"
     CDS             249..848
                     /locus_tag="P5219_10310"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit E
                     family protein"
                     /protein_id="HRX27199.1"
                     /translation="MYFGPFSSYEEYLKELERFHGKKAPGGVLALHMVSLARELLPEG
                     ILMDVICETRKCLADAIQLLTPCTVGNHWMKIVDTGRFAAVFYDKQTGEGVRISLGME
                     RLKEYPAVNEWFLKLVPKKDQDLDAILEGINRAGAALFDSSPVMVDPKVLQVRPKVPP
                     VICPVCGEAYPPYHGPVCRGCRGFTEAYFREPAGARTEK"
     gene            complement(926..>1433)
                     /locus_tag="P5219_10315"
     CDS             complement(926..>1433)
                     /locus_tag="P5219_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27200.1"
                     /translation="AGLPYLQLHKFVMALIMGIVFYETGFLEHKVLDKANAMGYAMFF
                     CLIFIWVSLPQATPELVRSLVYPLVVCFGVALVGIVTSTFFTSKILGYSWAMATAIAV
                     TCLFGFPATFIITDEVTTAGCADPKEKEYCMSHLLPKMLVGGFTTVTIGSVILAGYLG
                     QIIETLAK"
BASE COUNT          291 a          415 c          478 g          249 t
ORIGIN      
        1 tccgtgcagg acgccgagcg gctgggcatc gcccacggcg aggagatccg cgtggcgtcg
       61 cggcgcggcg aggtacgcat caaagcaaac gtctcaaagc gtatcgcccc ggggatggtc
      121 tggatgtcct tccacttctg ggagaccaac gccaaccacc tgctgagcgg agacctcaag
      181 catttcgatc cggacaccat gacgccctcc ttcaaggcgt gcgccgtgag gatcgagaag
      241 atcgcctgat gtacttcggt cccttttcct cctacgagga atatctgaaa gaactggagc
      301 gcttccacgg aaagaaagcc cccggaggcg tcctggcgct ccacatggtt tcgctggccc
      361 gggagctgct gcccgaaggc attctcatgg acgtgatctg cgagacccgg aagtgtctcg
      421 cagacgccat ccagctgctc accccctgca ccgtggggaa ccactggatg aagatcgtgg
      481 acacgggcag gtttgccgcc gtgttctacg acaagcagac cggcgagggc gtgcgtatct
      541 ccctcggcat ggaacggctg aaagagtacc cggccgtgaa cgaatggttc ctgaagcttg
      601 ttccgaaaaa ggaccaggac ctggacgcca tcctggaggg catcaacagg gcgggggccg
      661 cgcttttcga ctcgagcccg gtgatggtgg acccgaaggt gctccaggtc cgccccaaag
      721 tgcccccggt gatctgcccg gtctgcgggg aagcctaccc gccgtaccac ggtcccgtct
      781 gccgggggtg ccggggattc acggaggcct acttcaggga accggcgggg gctagaacgg
      841 aaaagtaaca gcgtcatttc accccaacgt aagagggagg tccggacatg tccggacctc
      901 cctcttacgt tggggtatgg agcgcttatt tcgccagggt ctcgatgatc tggccgaggt
      961 acccggcgag gatgacggag ccgatggtga ccgtggtgaa ccctcccacg agcatcttgg
     1021 gcaggagatg gctcatgcag tactccttct ctttcgggtc ggcgcatccc gccgtggtga
     1081 cctcgtcggt gatgatgaag gtcgccggga agccgaagag gcaggtgacg gcgatggccg
     1141 tcgccatggc ccaggagtat ccgaggatct tcgaggtgaa gaaggtggaa gtgacgattc
     1201 cgacgagggc gacgccgaag cagaccacca gcgggtagac cagggagcgt accagttccg
     1261 gtgtggcctg gggaaggctg acccagatga agatcaggca gaagaacatg gcgtagccca
     1321 tggcgttggc cttgtcaagg accttgtgct ccaggaagcc ggtctcgtag aacacgatgc
     1381 ccatgatgag ggccatgacg aacttgtgga gctgcaggta aggcagtccg gcg
//
LOCUS       DAOTHD010000673         1433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_107292_length_1433_cov_2.274311, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000673 DAOTHD010000000
VERSION     DAOTHD010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107292_length_1433_cov_2.274311"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="P5219_10320"
     CDS             <1..728
                     /locus_tag="P5219_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRX27201.1"
                     /translation="DDAFYRALRGRDLLDVKLRAIDNCARFNLGVVLVPTLVPGINTG
                     EIGNLIRFAVSRTPAVRGVHFQPVSHFGRIPALPSDSMRFTLDELLAAIEEQAGDLVP
                     KDSIIPSRCNHPLCGFHADYVVTSGGRLHPLHREGGQCCGKVVSADQNRSFIATKWER
                     PEPPESASRNDNDGEKGCCTADLKSFDGFLLFARRYSFTLTAMAFQDAGNLDLERLRR
                     CSLHVFDDGRFVPFCSYYLTAQG"
     gene            739..>1433
                     /locus_tag="P5219_10325"
     CDS             739..>1433
                     /locus_tag="P5219_10325"
                     /inference="COORDINATES: protein motif:HMM:NF026042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HRX27202.1"
                     /translation="MEKRIRDIKDRCLEKEDPFCASACPFRLDVREFIGRMQRGAFNS
                     AFRLFSNTVGFPAIVAALCDQPCRNVCPRGRVDAPISLNLLEKAAIAYAANTGPNSYN
                     LPPKQGRIAVIGAGISGLACALRLASKKYAVDVYERSGRVGGALWNVLDSAVFLEDIR
                     LQFSNEEYGLFLDHQVSGLAPLLEKYDGVYIATGRGGDSFGLERQAGGDEGVPLATGL
                     PGVFLGGSLTGATL"
BASE COUNT          257 a          454 c          443 g          279 t
ORIGIN      
        1 ctgatgacgc cttctacagg gcccttcgcg gacgggatct cctcgacgtc aaacttcggg
       61 ccatcgacaa ctgcgcacgg ttcaacctgg gcgtggtgct cgtcccgacc ctcgttccgg
      121 ggatcaacac gggggaaata ggcaacctca tcaggttcgc cgtttcccgc acccctgcgg
      181 tacggggcgt ccatttccag ccggtgagcc atttcggacg aattccagca cttccgtcgg
      241 acagcatgcg ctttactctc gacgagctgc tcgccgccat cgaggaacag gcgggggatc
      301 tggtgccgaa ggattccatc attccttccc gctgcaacca ccccctgtgc ggatttcatg
      361 ccgactatgt tgtcacgagc gggggcaggc tgcacccgct gcacagggag ggagggcagt
      421 gctgcggcaa ggtcgtttcg gccgatcaaa accggtcctt catcgccacg aaatgggagc
      481 ggcctgaacc tccggaatct gcttcccgga atgacaacga cggggagaag ggctgctgca
      541 cagcggacct gaaatctttc gacggttttc tcttgtttgc ccggaggtac agcttcacgc
      601 tgaccgccat ggcgttccag gatgcgggaa acctcgacct tgagcgcctg cgcaggtgca
      661 gtctccatgt ttttgacgac ggccggttcg ttcccttctg ctcctattac ctgacggccc
      721 aggggtgacg gggtccagat ggaaaaaaga atccgggaca tcaaggaccg ctgcctggag
      781 aaggaagacc cgttctgcgc ttcggcctgc ccgttccggc tggacgtcag ggaattcatc
      841 ggccggatgc agcggggcgc cttcaattcc gccttccgcc ttttctccaa cacggtgggt
      901 ttccccgcca tcgttgccgc cctgtgcgac cagccctgca ggaacgtttg ccccaggggg
      961 cgggtggatg cccccatctc gctgaacctg cttgaaaagg ccgccatcgc ctatgcggca
     1021 aacaccgggc cgaacagcta caacctgccc ccgaaacagg gcaggatcgc cgtgataggg
     1081 gcggggatca gcggccttgc ctgcgccctg cgcctggcga gcaaaaaata cgccgttgat
     1141 gtgtacgagc ggagcggccg cgtaggggga gccctgtgga acgtcctcga cagcgccgtt
     1201 ttcctcgaag atatccggct gcagttctcg aatgaggaat acggcctctt cctcgatcat
     1261 caggtctccg gactcgcccc tctgctggaa aagtacgacg gggtctatat cgccaccgga
     1321 agaggtgggg acagcttcgg cctggaacgg caagcgggcg gcgatgaagg cgttcctctg
     1381 gcgaccggcc ttccgggggt ctttctcggc ggttccctca cgggggcgac cct
//
LOCUS       DAOTHD010000674         1430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_107652_length_1430_cov_2.328727, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000674 DAOTHD010000000
VERSION     DAOTHD010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107652_length_1430_cov_2.328727"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /locus_tag="P5219_10330"
     CDS             <1..1398
                     /locus_tag="P5219_10330"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRX27203.1"
                     /translation="GEAAAFLARWAETASTRTSPPVSPAMAVHQGTPPVLTSAAVWVF
                     LGATAPHWPGQVRESPLLNDDRKGILHDSLGLGRSHLPLVPEKRKQREALFRRLAACA
                     RELCLFVRPLADGGGRPLPPSPFAAEAEAGPAPWLIPAETSYERSLGDLLPPAVGETV
                     CVRGVEIPAECSPRKGLFRRGHLTAPLPLPEKIFHLSGLDDFAACPFLYYCRRMGLEP
                     AAEELFRRDLAGNGFHRLWELAWKEHLSSGGSLEELADRFFDAAYGDGYPGLLSDQRL
                     FRHRADQREKNRRLGALQDAMERSGLGEARSEQRREFPLPELEIGGTVFRGRCDRMDI
                     LRDGSALLFDYKSGRSGKYTKSLQLAAYSIALREGPERRSASAAVFLGLGDGSAAGAK
                     HDGAPLWLDAGKISLAELESQAAEVMKRAADSIASGDFPPEYDSDRCRYCSFSSLCRR
                     RDFRAEEEDNGEGEE"
BASE COUNT          231 a          470 c          503 g          226 t
ORIGIN      
        1 ggagaggctg cggccttcct ggccaggtgg gctgagacgg cctccacgag gacttcgccg
       61 ccggtctccc cggccatggc ggttcaccag ggaacgccgc cggtgctgac ttcggcggcg
      121 gtgtgggtct tcctcggcgc cacggccccc cactggccgg gccaggtgcg ggaatccccg
      181 cttctgaacg acgaccggaa ggggatcctc cacgacagcc tcggtttggg gcggagccac
      241 ctccccctgg tgccggagaa gcggaagcag cgggaagccc tcttccggag acttgccgcc
      301 tgcgcacggg aactgtgcct tttcgtccgc cccctggccg acggtggcgg gcggcctctg
      361 ccgccgtccc cattcgcggc ggaagcggag gccggaccgg ccccctggct tatcccggcg
      421 gaaacttcct atgaacggtc cctgggggat cttctccccc cggcggtcgg agaaacggtg
      481 tgtgtcagag gcgtggaaat tcctgccgaa tgcagccccc gaaagggcct ttttcggagg
      541 ggtcacctca ccgcacccct ccccctgccc gagaagatct tccatctgag cggcctggac
      601 gacttcgccg cctgcccctt cctgtactac tgccggagga tgggcctcga gcctgcagcg
      661 gaggagctgt tccgccgcga cctggcgggg aacgggttcc accgtctctg ggagctggcg
      721 tggaaggagc acctttcctc cgggggaagc ctggaggagc tggcggaccg ctttttcgac
      781 gccgcctacg gggacgggta ccccgggctg ctctcggacc agaggctctt ccgccacagg
      841 gctgaccagc gtgagaaaaa ccgccgcctc ggggcgcttc aggacgccat ggagaggagc
      901 ggcctcgggg aggcccggag tgaacagcgg cgggaattcc ccctgcctga gctggagatc
      961 ggggggacgg tcttccgggg acggtgcgac agaatggaca tcctccgcga cggttccgcc
     1021 ctcctcttcg actacaagag cggcaggtca gggaaataca caaaaagcct ccagctcgcc
     1081 gcctacagca tcgccctccg ggaaggcccg gaacggcgga gtgcctccgc ggcggtcttt
     1141 ctcggactgg gcgacggctc ggcggcgggc gcgaagcatg acggcgcgcc cctctggctg
     1201 gacgcgggaa aaatatctct tgcggaactg gagtcccagg cagcggaggt catgaagcgg
     1261 gcggcggact ccatcgcttc gggggacttt ccgccggagt acgattcgga ccgatgcaga
     1321 tactgctctt tctcctccct ctgcaggcgc agggatttcc gggcggagga agaggataac
     1381 ggggaagggg aggaataggc catggcggac ggcaacctga tgaccggacg
//
LOCUS       DAOTHD010000675         1429 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_107722_length_1429_cov_3.299127, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000675 DAOTHD010000000
VERSION     DAOTHD010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107722_length_1429_cov_3.299127"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="P5219_10335"
     CDS             <1..688
                     /locus_tag="P5219_10335"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRX27204.1"
                     /translation="FASILSTLESMRLGVPALLARSAQYIMLALILDGLDGNLARWLK
                     GSTDFGAELDTFVDFSAFGAAPAVLMYALLLHGPSPFWRTILPSAIVFSGALRLSKFR
                     LKDPLRGQGGFIGLPITVNSSWIALSAFAAQVLYPDDHILVTGPVSLVFQFLILVMIS
                     LQLSNLRYPKPSNKVKYFAPAALVVLLLWILSDTYAAWIASILVLGCLVYVLTGPMIH
                     KRSAPKRVEQ"
     gene            696..>1429
                     /locus_tag="P5219_10340"
     CDS             696..>1429
                     /locus_tag="P5219_10340"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRX27205.1"
                     /translation="MKAVGVDLGGHKIAAALVEDGHILRRISEPTSGREPDGVLSQIA
                     AMAKSLGACSGCPVGVGIPGVLDAARETALLLPNFSGWNGLPLRKMLSSKIGLPVAME
                     NDANCHALGEGWGGAAVGMDDFLLLALGTGIGGAIVIGGRILKGFHGMAGEPGHLVVG
                     SGEPCGCGSHGHLEGISGADALERQATAMGLQPDLKYLWTRRTDPAVAPLWDRGLDHL
                     AKGIASAIHLLDPQAVILGGGLSRGE"
BASE COUNT          224 a          515 c          429 g          261 t
ORIGIN      
        1 cttcgccagc atcctttcca ccctggaatc catgcggctc ggcgtgccgg ccctcctggc
       61 ccgctccgcc cagtacatca tgctcgccct catcctggac ggactggacg gcaacctcgc
      121 ccggtggctc aagggctcca ccgacttcgg cgccgaactc gacaccttcg tagacttttc
      181 ggccttcgga gccgccccgg cggtgctgat gtacgccctg ctgctccacg ggcccagccc
      241 cttctggaga accatcctgc catcggccat cgtcttctct ggggccctgc ggctttccaa
      301 gttccgtctg aaggaccccc tccggggcca gggaggcttc atcggcctcc ccatcaccgt
      361 caactcgtcg tggatcgccc tttccgcctt tgccgcccag gtactgtacc cggatgacca
      421 catcctggtc acgggtccgg tctccctggt tttccagttc ctgatcctcg tcatgatctc
      481 cctgcagctc tcgaacctcc gctaccccaa gccgtcgaac aaggtgaagt acttcgcccc
      541 tgcggccctg gtggttcttc tgctgtggat tctcagcgac acgtacgcgg cgtggatcgc
      601 ctccattctc gtgctgggat gccttgtgta cgtccttacg ggacccatga tccacaagcg
      661 ttccgcaccg aagcgggtgg agcagtagac cggccatgaa agcggtggga gtggacctcg
      721 gaggacacaa gatagccgcc gccctcgtgg aggacggcca tatcctgcgg aggatctccg
      781 agcccacttc cgggcgggaa cctgacgggg ttctctccca gatcgccgcc atggcgaagt
      841 cccttggtgc ctgcagcggc tgcccagtgg gagtcggcat cccgggggtg ctcgacgccg
      901 cccgggagac ggccctgctc cttcccaact tttcgggctg gaacggcctt cccctccgga
      961 aaatgctctc ctcgaaaatc ggcctccccg tggccatgga aaacgacgcc aactgccacg
     1021 ccctcggcga gggatggggc ggagccgccg tcggcatgga cgattttctt ctcctggccc
     1081 tcggaacggg catcggcggg gccatcgtca tcggcggaag gatcctgaag ggctttcacg
     1141 gcatggcggg agaaccggga cacctggtgg tggggtcggg cgaaccctgc ggctgcggct
     1201 cccacggcca cctcgagggg atcagcggag ccgacgccct cgagcgccag gccacagcca
     1261 tggggctcca gcctgacctg aaatacctgt ggacccgcag gaccgaccct gcggtagccc
     1321 ccctctggga caggggactc gaccacctcg ccaagggcat cgcctcagca atccacctcc
     1381 ttgaccccca ggccgtcatc ctcggcggag gcctgagcag gggagagga
//
LOCUS       DAOTHD010000676         1428 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_107827_length_1428_cov_4.400583, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000676 DAOTHD010000000
VERSION     DAOTHD010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107827_length_1428_cov_4.400583"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="P5219_10345"
     CDS             complement(<1..198)
                     /locus_tag="P5219_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRX27206.1"
                     /translation="MKRFLVVLAVLSVMASSLPAAAAPEMVLRLGETHVADYPTTKGD
                     MEFARLVEERTGGRIKIEVYHS"
     gene            complement(301..>1428)
                     /gene="uxaC"
                     /locus_tag="P5219_10350"
     CDS             complement(301..>1428)
                     /gene="uxaC"
                     /locus_tag="P5219_10350"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF002794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HRX27207.1"
                     /translation="IVRNPLYHWTHLELQRVFGVNELLNPGTAGAIYSHCTALLGKKE
                     FSARSIIRKFRVEALCTTDDPSSSLEEHRQIRESGFETIVAPAFRPDRALEGHLPERF
                     NPWLDRLAELAEVKIDSFGRFVECLWKRHRFFHDNGCRLSDYGIETCYSSDWTSEEVE
                     SSFAVLRSGRALSGEPLLRFRSAVLYELLSMDASQGWTQQLHLGALRSTNSRMLAKLG
                     PDTGYDAMGDFEQGRPLARLLDRLERTGKLARTIVYSLNPRDNDMIPSILGCFQDGQV
                     PGKMQMGSAWWFTDTKDGMTRQIEALSSVGVLSGFVGMLTDSRSLLSYPRHEYFRRIL
                     CNVIGGDVERGELPEDYALLGGMVRDICWNNAKEYFRLHSA"
BASE COUNT          276 a          420 c          448 g          284 t
ORIGIN      
        1 gctgtggtag acctcgatct tgatcctgcc cccggtgcgc tcctcaacga ggcgggcgaa
       61 ttccatgtcg cccttggtgg tggggtaatc cgccacgtgg gtttcgccca gacggagcac
      121 catctccgga gccgccgccg ccggaaggga agaggccatt accgaaagaa ccgcaagaac
      181 cacaaggaac cgtttcatcc aatctcctcc tttaaatttc gaaatagaaa cctttctcat
      241 tgtagtcggc ttattccgaa aacgcaatca gaaaccggct gtgaatgaaa gtgtgttttg
      301 tcaggcggaa tgaagccgga aatactcctt cgcgttgttc cagcagatgt cccggaccat
      361 gcctccaagg agggcatagt cctcgggaag ctcgcccctt tctacgtcgc cgccgatgac
      421 attgcagagg attctgcgga agtactcgtg tctcgggtat gagagaaggc tccgggagtc
      481 ggtgagcatc cccacgaatc ccgagagcac gccgacggag gagagagcct cgatctgccg
      541 ggtcatgccg tccttcgtgt cggtgaacca ccaggcggat cccatctgca tcttccccgg
      601 cacctggccg tcctggaagc atcccagtat ggaggggatc atgtcgttgt ccctgggatt
      661 gagggaatac acgatggtcc tggcgagctt tccggtccgc tccagcctgt cgagcagccg
      721 ggcgaggggc cgtccctgtt cgaaatcgcc catggcgtcg tatcctgtgt ccggcccgag
      781 ctttgccagc atcctcgagt ttgtgctgcg cagcgctccc aggtggagct gctgggtcca
      841 tccctgggag gcgtccatgg acagcagctc gtagagcacc gcggaccgga acctgaggag
      901 gggttccccg gagagggcgc ggccgctcct caggacggcg aaggaggatt ccacctcctc
      961 cgatgtccag tcggaggaat agcatgtttc gatgccgtag tcggatagac ggcagccgtt
     1021 gtcatggaag aaccggtgcc tcttccagag gcattcgacg aaccggccga aggagtcgat
     1081 cttgacttcg gccagttccg ccagtctgtc gagccagggg ttgaaccgtt cgggaagatg
     1141 cccctccagc gccctgtcgg gccggaaagc tggagccacg atggtctcga agccgctctc
     1201 ccggatctgc cggtgctctt cgagggagga tgaaggatca tcggtggtgc agagagcctc
     1261 caccctgaac ttcctgataa tggaccgggc cgagaattcc ttcttcccga ggagggcggt
     1321 gcaatgactg taaatcgccc ccgcggtgcc ggggttcagg agctcgttca cgccgaagac
     1381 cctctggagc tcgaggtggg tccagtggta caggggattg cggacgat
//
LOCUS       DAOTHD010000677         1427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_108001_length_1427_cov_2.420554, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000677 DAOTHD010000000
VERSION     DAOTHD010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108001_length_1427_cov_2.420554"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..235)
                     /locus_tag="P5219_10355"
     CDS             complement(17..235)
                     /locus_tag="P5219_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27208.1"
                     /translation="MGTETKRNPRKAQRPSALAGQIRIIFYTVFICITIVLLAVSLDL
                     MLNPSKNWTDALAIRWISERRNMLPFLK"
     gene            336..>1427
                     /locus_tag="P5219_10360"
     CDS             336..>1427
                     /locus_tag="P5219_10360"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRX27209.1"
                     /translation="MQNFTFYAPTKIIFGRDTIPQIGEALAADGISRAVLVAGGGSIF
                     KNGVHEAVTESLKKHGIHSAEIGGVRSNPRLSKLRDVITLLKGEKAEAVLAVGGGSVF
                     DTAKAAAMGALYDGDVWDFFCGKAAVKEALPVYGVLTASGTSSEMNNTAVVTREEDES
                     KWAIASPVLVPKVSVIDPSVQATLPESQTVSGGIDAITHVLEAYVISCPGLDFVQDYC
                     ESLIRAMMRRIPQLVKNPGDYEARAELAWCCTLAHNGLSNVGRPARGDFASHRIEHSL
                     SAIFDVPHGTGLAIIMPAWMEYVYRDAEPVFERLAANVFGVRDGEGRALRGIHALREY
                     FRSLGSPVTLREVGVTKADLPGIADNAAKL"
BASE COUNT          285 a          427 c          452 g          263 t
ORIGIN      
        1 gggcgggaaa aagaggctat ttcaaaaacg gcagcatgtt ccgacgttcg gatatccagc
       61 ggatggcaag ggcatcggtc cagtttttcg acgggttgag catgagatcg agggagacgg
      121 cgaggagcac gatggtaatg cagatgaaaa ccgtgtaaaa gatgatgcgg atctgccccg
      181 caagggctga gggccgctgc gctttccgcg ggttcctttt cgtttccgtt cccatgatat
      241 catctccttc gttctacgcc gccattatac atcctccggc ttccgggggt acaatactgt
      301 ccatgccggg atttctccgg cgaaaggagg aaaacatgca gaatttcacc ttttatgctc
      361 cgacaaaaat catcttcggc agggatacca ttccccagat cggcgaggcc cttgcggcgg
      421 acggaatatc ccgggcggtt cttgtcgccg gagggggctc catcttcaaa aacggcgttc
      481 acgaggccgt gacggagtca ttgaagaaac acggcatcca cagtgccgaa atagggggcg
      541 tccggtcgaa ccccaggctg tccaagctcc gggacgtcat caccctgctg aagggggaaa
      601 aagccgaagc cgtgctcgcc gtgggcgggg ggagcgtctt cgacactgcg aaggcggccg
      661 ccatgggggc tctctacgac ggggacgtct gggacttctt ctgcgggaag gcagcggtga
      721 aggaagccct gcccgtctac ggagtgctca ccgcgtcggg cacctcctcg gaaatgaaca
      781 acactgccgt ggtgaccagg gaggaggatg agagcaagtg ggcgatagcg tcccctgtcc
      841 tcgtccctaa agtgtccgtc atcgacccgt cggtgcaggc gactctcccg gagagccaga
      901 cggtgagcgg cggtattgac gccatcaccc acgtcctcga ggcgtacgtg atctcctgcc
      961 cggggctcga tttcgtccag gactactgtg aatccctcat tcgggcgatg atgcgccgga
     1021 ttccccagct cgtgaagaat ccaggagact acgaggccag ggcggaactt gcctggtgct
     1081 gcaccctggc ccacaacggc ctgagcaacg tgggccggcc cgcccgggga gatttcgcct
     1141 cccaccgcat cgaacactcc ctgagcgcca tcttcgacgt accccacgga acggggcttg
     1201 ccatcatcat gcccgcctgg atggaatatg tgtacaggga cgcggagccc gtgttcgaac
     1261 ggctcgccgc caatgtcttc ggcgtccggg acggcgaagg ccgggccctg agaggcattc
     1321 atgcgctgcg ggagtacttc cgctccctcg ggtctcccgt gaccctccgg gaagtagggg
     1381 tgacaaaggc cgatctgccc gggatcgccg acaacgcggc caagctc
//
LOCUS       DAOTHD010000678         1427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_108020_length_1427_cov_2.036443, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000678 DAOTHD010000000
VERSION     DAOTHD010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108020_length_1427_cov_2.036443"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..>1427)
                     /locus_tag="P5219_10365"
     CDS             complement(165..>1427)
                     /locus_tag="P5219_10365"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRX27210.1"
                     /translation="GDPRIFTRDDLDDLVPSNPVILSRVCGHVAVLNSMALEILGITA
                     DSRFSGGVVDLDDTGRPTGVIRETAVEWAHSRTPNPDGDKLRRLVARAGAAAAAAGLT
                     SIHSDDLGSVGGDWGAILDLYLGLDGEGKMPVRITEQLLLRNRAALDRFLASGWRTGD
                     GSPAFHIGPLKILTDGSMGGRTAFLREEYSDMPGVYGVPIYTAGELNDLVWTAHSAGM
                     QVALHAIGDGALDMCLDALEKARERNLRKARHYIVHCQTGDMEQYHRMARLGVGAAIQ
                     PPFVPSDRPMAVRRIGEDRARRGYAWKTLLDLGIFLSGGSDCPVETFDPLWGIYTAVT
                     RKDRDGVPEGGWNPAQKLTVEEAVDLYTRGGAYVSFEEHRKGTLQAGKLADLVVLDRD
                     IFSVPEEGIPAAKPVLTMMGGKITFRDI"
BASE COUNT          218 a          484 c          471 g          254 t
ORIGIN      
        1 cggttcgccg gccatatccc cccgtccatg tgggacaaag aggcgggatg cctccaccgg
       61 ggcatcctgg ccttcggcgc cggcgggacg ttcctggccc ggaacaggga cctccctctc
      121 ccggggggag gggaggtccg gttccatctt cccctgtccg gaggctagat gtcccggaag
      181 gtgatctttc cgcccatcat ggtcaggaca ggcttcgcgg cgggaatccc ctcttcgggg
      241 acggagaaga tgtccctgtc gagcacgaca aggtcggcga gcttcccggc ctgaagggtt
      301 ccctttctgt gctcttcgaa ggaaacatag gcccctcccc tggtgtagag atccaccgct
      361 tcctccacgg taagcttctg ggccgggttc caccctcctt cgggaacacc gtcccggtct
      421 ttgcgcgtga cggccgtata tatgccccag aggggatcga aggtctccac ggggcagtcc
      481 gagcctcccg agaggaaaat gcccaggtcc aggagggtct tccaggcgta ccccctgcgg
      541 gcccggtcct ccccgatcct ccggacggcc atggggcggt ccgaggggac gaagggaggc
      601 tggatggcgg ctcccacgcc gaggcgggcc attctgtggt actgctccat gtcccccgtc
      661 tggcagtgga cgatgtagtg gcgggccttc ctcaggttcc gctccctggc cttttccagg
      721 gcgtcgagac acatgtccag ggcgccgtcg ccgatggcgt ggagggctac ctgcatcccc
      781 gcgctgtggg cggtccagac gaggtcgttc agctccccgg cagtatagat gggcacgccg
      841 tacactcccg gcatgtctga gtattcctcc cgcaggaagg ccgttcttcc ccccatggac
      901 ccgtcggtga ggattttcag gggaccgatg tggaaagcgg gactgccgtc cccggtcctc
      961 caccccgaag ccaggaaccg gtcaagggcc gccctgttcc tgaggaggag ctgctcggtg
     1021 atcctgacgg gcatctttcc ttccccgtcc aggccgagat acaggtcgag aatggctccc
     1081 cagtcccccc ccacggaccc caggtcgtcg gaatggatgg aggtcagtcc agccgccgcg
     1141 gccgccgcgc ctgccctggc cacaagacgc cggagcttgt ccccgtccgg gttgggcgtc
     1201 ctggaatggg cccattcaac ggcggtctcc cggatcaccc cggtggggcg tcccgtgtcg
     1261 tccaggtcca ccactccgcc ggaaaaacgg ctgtcggcgg tgatccccag gatctccagg
     1321 gccattgagt tgagcaccgc cacgtggccg cacacccggg acaggatcac ggggttggag
     1381 gggacgaggt cgtcaaggtc gtcccgggtg aagatccggg ggtcccc
//
LOCUS       DAOTHD010000679         1426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_108110_length_1426_cov_2.313640, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000679 DAOTHD010000000
VERSION     DAOTHD010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108110_length_1426_cov_2.313640"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1426
                     /locus_tag="P5219_10370"
     CDS             <1..>1426
                     /locus_tag="P5219_10370"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRX27211.1"
                     /translation="IGRSRSCDIVLDDPSVSRHHCRVEVFPGHIRLVNEKSRNGTTVD
                     HQPVEEAILALNQSFGVGGHEFFYKEGDVSEFRLSSELAWMSGHLLRARPGGQSEAET
                     REGGEKYSVLLDELSAKALLCETGESFLKAVSNSLAGLFPQSALLLENGPGRTVILDQ
                     TLRDTVSLGTQTLSAEPEGSLRDESGALHFRRYHPSTSPAGYSLVLLHELRLPHRGGP
                     GDLFFMKLAELILFNSSLSAAPPDLRPETVYQGQDLTIIGRSPGLRQICAMMQKIAPK
                     ASFILIMGENGSGKELFARMIHQLSGRKRYVAVNCAAIPTTLLESELFGHEAGAFTDA
                     KKRRIGRMEEASGGTLFLDEIGDMPPEIQAKLLRTIQERALCRLGGSEMIPLDLRIIA
                     MTNRDLYRLVEEGRFRQDLFFRLRVHEFTVPPLRERPEDIVPLVSHFTRLYAQRNGIR
                     PAGFSESAGACLQAYLWPGNVRELE"
BASE COUNT          258 a          467 c          442 g          259 t
ORIGIN      
        1 atcggtcgaa gccggagctg cgacattgtc ctcgacgatc cgtcggtctc ccggcaccac
       61 tgccgggtgg aggtctttcc gggacacatc cggctggtga acgagaagtc gagaaacggc
      121 accaccgtgg accaccagcc ggtggaggag gcgattctgg ccctgaatca gtccttcgga
      181 gtcggcggac acgagttctt ttataaagag ggggacgtct cggagtttcg cctcagttcc
      241 gaactggctt ggatgtcggg acacctgttg cgtgcacgac cgggcggcca gtcggaggcc
      301 gagacccggg aaggaggcga gaaatacagc gtcctgctgg acgaactgag cgccaaggcc
      361 ctgctctgcg agactggcga gtccttcctg aaagccgtgt ccaactccct ggccgggctc
      421 tttccccaga gtgccctgct gctggagaac ggaccggggc gaaccgtcat cctggaccag
      481 acccttcggg acacggtctc tctgggaacg caaacactgt ccgccgagcc cgaaggctca
      541 cttcgcgacg aatccggagc actgcatttt cggcgctatc acccttcgac ctctcccgct
      601 ggctattccc tggtgctgct gcacgaactg cggctgcccc atcggggggg accgggggac
      661 ctttttttca tgaagctggc cgagctcatc ctgttcaatt caagcctgtc ggcggctcct
      721 cccgatcttc ggccggaaac ggtttaccag gggcaggact tgaccatcat cggccgcagt
      781 cccggtctgc ggcagatctg tgccatgatg cagaagatcg cccccaaggc cagtttcatc
      841 ctgatcatgg gtgagaacgg cagcggcaag gaactcttcg cccgcatgat ccaccagctt
      901 tcgggacgca aacgctatgt ggccgtcaac tgcgccgcca ttccgaccac cctgctggag
      961 agtgaactct tcggccacga ggccggagcc ttcaccgacg ccaaaaagcg ccgcatcggc
     1021 cgcatggagg aggcgtcagg gggaaccctg ttcctcgacg agatcggcga catgcccccc
     1081 gaaatccagg ccaagctgct gcggaccatc caggagcggg ccctgtgccg cctggggggg
     1141 agcgagatga tccccctgga cctgcgcatc atcgccatga ccaaccgcga cctctaccgc
     1201 ctggtggagg agggccgttt ccgtcaggac cttttctttc gcctgcgggt gcacgagttc
     1261 accgtgccgc cgctgcggga acggcccgag gatatcgtac ctctggtttc ccacttcacc
     1321 cgtctgtacg cgcagcgcaa cggcatccgt ccggcgggat tctccgagag cgccggcgcc
     1381 tgtctgcagg cctacctctg gccgggaaac gtccgggaac tggaga
//
LOCUS       DAOTHD010000680         1424 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_108362_length_1424_cov_2.578524, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000680 DAOTHD010000000
VERSION     DAOTHD010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108362_length_1424_cov_2.578524"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="P5219_10375"
     CDS             complement(<1..216)
                     /locus_tag="P5219_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HRX27212.1"
                     /translation="MKKVFGLLLVAVMLFGLCAPVLAGSGKVAIVTNTVSQNEEEYRS
                     AQEMVTKYGDRIVHVTWPDNFMAEQEQM"
     gene            600..>1424
                     /locus_tag="P5219_10380"
     CDS             600..>1424
                     /locus_tag="P5219_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HRX27213.1"
                     /translation="MINRKRLEALAEELKKLELDAMYLGPSTDLEYISGLDTHPDERV
                     RGLMVSKDGRCFAMTPLLYKEEMVNAFGDVPFYAEWNDHEGFTGAFRRGCEHLGVVGG
                     KIAFNDGVRAVDMLAVRDTMDLRMCNGAGILDPLRSRKDEDELAVMRAASRIVDAVVG
                     KLLDFIRPGMTERQIAKKIPEFFEEAGSNEISFSPIVASGPNGSMPHYGGDRRVIEEN
                     DVIILDLGGRYKSYCSDTTRTFFTGTPTEEQKKVYEIVRQAQAAGEAAVQPGATGQD"
BASE COUNT          347 a          402 c          391 g          284 t
ORIGIN      
        1 catctgctcc tgctccgcca tgaagttgtc gggccacgtc acgtggacga tcctgtcgcc
       61 gtatttcgtg accatctcct gggcggaacg gtactcttcc tcgttctgag aaacggtgtt
      121 ggttacaatc gcaaccttcc cggagccggc aagaaccggt gcgcaaaggc cgaagagcat
      181 gactgcaacc agcagcaaac caaatacctt cttcattcat ttccctccct ttcacatgca
      241 aaagttccgg ctgtttctgc agaaataacc ggcagtgctg caatcatatt cctgacccat
      301 tctttcgtca acactactgg aatttcccat aaagcatacc atcattcata tatctttaaa
      361 aaaaataaag aaatagatat aattcatggc atacaataaa ggatttttaa aatttcagtc
      421 ggtccgttac cagttctatc cttacggtgg tatgcctttc gtatggatta tgaataagag
      481 aagtattatt atggcgagag aaaaaaaccg gacgggggca aatcatcccg ttccggaaat
      541 tccgctccgc aggcagggaa tgggctttct gcgggcaaaa ccacttcagg aggatatcca
      601 tgatcaatcg gaagagactg gaagcgcttg cagaagaact gaagaaactg gagctcgacg
      661 ccatgtacct cggcccttcc acggacctgg agtacatcag cgggctggac acccaccccg
      721 acgaaagggt cagggggctc atggtgtcga aggacggacg gtgtttcgcc atgacgcccc
      781 tgctgtataa ggaggagatg gtcaacgcct tcggagacgt ccccttctac gccgaatgga
      841 acgaccacga gggattcacg ggcgccttca ggcggggctg cgaacacctg ggcgtcgtcg
      901 ggggaaagat cgccttcaac gacggcgtca gggcagtgga catgctcgcc gtacgggaca
      961 ccatggatct ccggatgtgc aacggggcag gcatcctcga ccctctccgc tcccggaagg
     1021 acgaggatga actggccgtc atgcgggcgg cgtcccgcat tgttgatgcc gttgtgggca
     1081 agctgctgga tttcatccgg ccgggcatga cggaacggca gatcgccaaa aagattccgg
     1141 aattcttcga ggaagcgggc tccaacgaaa tatccttctc tcccatcgtg gccagcgggc
     1201 ccaacggctc catgccccac tacggcggcg accggagggt catcgaagaa aacgacgtga
     1261 tcatcctcga cctcggcgga cggtacaagt cctactgctc cgacactacc cggaccttct
     1321 ttaccggcac ccccacggag gagcagaaga aggtgtacga gatcgtcagg caggcccagg
     1381 ccgcgggaga agccgccgtg cagcccggcg ccacgggaca ggat
//
LOCUS       DAOTHD010000681         1421 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_108757_length_1421_cov_2.074671, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000681 DAOTHD010000000
VERSION     DAOTHD010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108757_length_1421_cov_2.074671"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="P5219_10385"
     CDS             <1..745
                     /locus_tag="P5219_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HRX27214.1"
                     /translation="LVKDGNLELYDGRIRLKSPRGRITDEFDDKDYCAHIGEHVEEWS
                     YLKFPFYRPLGFPHGSYRVGPLGRINACDDIATPEASAELAAFRKSTDDGMVHYTLYY
                     HYARLIEILYSMERLEELLNDPDVLGSDLRITGNVTNREGVGVVEAPRGTLIHHYEVD
                     GKGAIERANLIVATGHNNYAMNRGVEQVARDYITGSDVKEGALNRMEHVIRCYDPCLS
                     CSTHAVGKMPLKLELRNSRGDVVRTVTKE"
     gene            742..1206
                     /locus_tag="P5219_10390"
     CDS             742..1206
                     /locus_tag="P5219_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HRX27215.1"
                     /translation="MKVLVCGYGNPFRTDDGVGHLLAPAVAELIASRGGEAEVRLEHQ
                     LLPEMAEEFGKYDLLAFTDARVPGTGSGKGYDIREISPDPALEGLNIHSVGPEWILAL
                     AARLWGKVPPSVLVSVEGESFDFGEGLTAACNERMNCALAAFDEWLRDRNLQ"
BASE COUNT          304 a          428 c          470 g          219 t
ORIGIN      
        1 cctcgtgaag gacggaaacc tggagctcta cgacggccgc atccgcctca agagccccag
       61 ggggcggatc accgacgagt ttgacgacaa agactactgc gcccacatcg gggaacacgt
      121 ggaggagtgg agctacctga agttcccctt ctaccggccc ctgggcttcc cccacggctc
      181 atacagggtg ggccccctgg gacgaatcaa cgcctgcgac gacattgcca cccccgaggc
      241 atcggcggag ctggcagcct tcaggaagtc caccgatgac ggcatggtcc actacaccct
      301 ctactaccac tacgccaggc tcatcgagat cctctattcc atggagcgtc tcgaggagct
      361 cctgaacgac ccggatgtcc tcggcagcga cctcaggatt accgggaacg tcacgaaccg
      421 cgagggcgtc ggtgtggtgg aggcccccag gggaaccctc atccaccact acgaggtgga
      481 cggcaaggga gccatcgagc gggcgaatct catcgtggcc accgggcaca acaactatgc
      541 catgaaccgg ggcgtggaac aggtggccag ggactacatc acgggctccg acgtgaagga
      601 aggggcactg aaccgcatgg agcacgtgat ccgctgctac gacccgtgcc tctcctgctc
      661 tacccacgcc gtcggaaaga tgcccctgaa gctcgagttg cggaacagcc gcggcgacgt
      721 ggtgaggacg gtcaccaagg agtgaaggtc ctcgtctgcg gctacggcaa ccccttccgg
      781 accgacgacg gtgtggggca tcttctcgcc ccggccgtgg ctgaactgat cgcctcccgt
      841 ggaggagagg cggaggtccg cctggagcac cagcttctgc ccgaaatggc ggaggagttc
      901 gggaagtacg atctgctggc cttcaccgac gcacgggtgc ccggtaccgg gagcgggaaa
      961 ggatacgaca tccgggagat ttctcccgac cccgcccttg agggactcaa catccactcg
     1021 gtaggcccgg agtggattct cgccctcgcc gcgagactat ggggaaaagt gccaccgtcg
     1081 gtactcgtca gcgtggaggg ggaatccttc gatttcggcg aaggcctgac tgcggcatgc
     1141 aatgaacgga tgaactgcgc cctcgccgct ttcgatgaat ggctgagaga cagaaattta
     1201 cagtagcagg cagcgcagaa cccccggcgg aagccggggg tttttttctg tgaagccggg
     1261 gggggggata ctccggcgga tgaatatcgg aatataatga atcaaaagac aggccccttc
     1321 cgcagaaggc ggcgggagga aaacaggggc ggtccggcag acggcgcgaa aggcggcgaa
     1381 gggcatggga agagcggcaa aaggggttgc ggcggtcctg a
//
LOCUS       DAOTHD010000682         1419 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_108978_length_1419_cov_2.233871, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000682 DAOTHD010000000
VERSION     DAOTHD010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108978_length_1419_cov_2.233871"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="P5219_10395"
     CDS             <1..412
                     /locus_tag="P5219_10395"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRX27216.1"
                     /translation="VAEVVPHLTESQALSLRRMEDEQLEAAEKGLAEQGSRYELPNMN
                     FELAITSLLDNPVIWEMHKKVESLWKKTFRRLSTTPFPARERYNHHMEIIRTMESGNI
                     KAAVKAMAFHNSILADYIDEEIRKLDSRRDEACG"
     gene            472..861
                     /locus_tag="P5219_10400"
     CDS             472..861
                     /locus_tag="P5219_10400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HRX27217.1"
                     /translation="MQFISTENAPRPGGHYSQAVVHRGIVYAAGQLPIVPETGEKCLG
                     TVEEQTERALMNLEAVLEAAGSGRDRVLRVTVYVSDISLWNRVNAVYAEFFGDHRPAR
                     TVVPTRDLHYGFLVEIDAVAATNSGTD"
     gene            889..>1419
                     /locus_tag="P5219_10405"
     CDS             889..>1419
                     /locus_tag="P5219_10405"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRX27218.1"
                     /translation="MKDLYSSLDTPSLLVDRTILLRNITGMQDLADRAGVALRPHTKT
                     HRTPAIAAMQVKAGAKGITVAKIGEAEVMAENGMDDIFIANEIYGDLKFARLRNLAAK
                     TRLSVGVDNREQVTAISRFFEGAREPANVLIEVETGEVRSGMLPGPELVELAKFIAAT
                     PNVRLKGIFSHEGHTYG"
BASE COUNT          333 a          398 c          455 g          233 t
ORIGIN      
        1 cgtggcggag gtggtccccc atctgacgga atcgcaggcc ctttccctcc ggcggatgga
       61 ggacgagcaa ctcgaggcgg ccgaaaaagg cctggccgaa cagggatccc ggtacgagct
      121 gcccaacatg aatttcgagc tggccatcac ctccctcctt gacaacccgg tgatctggga
      181 aatgcacaag aaagtggaat ctctgtggaa aaaaacattc cggaggctgt ccactacgcc
      241 ctttccggca agagagcggt acaaccacca catggagatc atcaggacca tggaaagcgg
      301 caatataaag gccgccgtga aagccatggc tttccacaac agcatcctgg ccgactacat
      361 tgacgaagag atcagaaagc tggattcacg gcgggacgag gcctgcggct aggatcagga
      421 aaagcgggac ggaacatcga cttctattct gaacggaggt gccggtatag tatgcagttc
      481 atttcgacag aaaacgctcc ccggccggga ggccattatt cccaggcagt ggtgcaccgg
      541 ggtatagtgt atgcggcggg gcagcttccc atagtgccgg aaacaggcga gaaatgcctg
      601 ggcacagtgg aggaacagac ggaacgggcc ctcatgaacc tggaggctgt cctggaggct
      661 gcgggaagcg gcagggacag ggtgctccgg gtcaccgtct acgtctcgga catttcgctc
      721 tggaacaggg tgaatgccgt gtatgccgaa ttcttcgggg atcatcggcc ggcgcggacc
      781 gtggtgccga cacgggatct gcactacgga ttccttgtgg aaatcgacgc cgtcgcggcg
      841 acgaattcag ggaccgactg atgcctgaag aacggaggaa cgacagcaat gaaagacctg
      901 tacagcagcc ttgatactcc ctccctcctt gtggacagga ccattctgct ccgcaatata
      961 acgggaatgc aggatctggc tgacagggcg ggtgtcgccc tgagacccca cacgaagacc
     1021 cacaggaccc ccgcgatcgc ggccatgcag gtgaaggccg gagcgaaggg catcacagtg
     1081 gccaagatcg gcgaggcgga agtcatggcc gaaaacggca tggacgacat tttcatcgcc
     1141 aacgagatct acggcgacct gaagttcgcc cggctccgga acctggccgc aaaaacgcgg
     1201 ctcagcgtgg gggtggacaa ccgggagcag gtgacggcca tctccaggtt cttcgaggga
     1261 gcccgggaac ccgccaatgt cctgatcgag gttgagaccg gcgaagtgcg gtcgggcatg
     1321 cttcccggac cggagctggt ggagcttgcg aaattcatcg ccgccacgcc gaacgtgcgg
     1381 ctgaagggca ttttttccca cgaggggcac acctacggg
//
LOCUS       DAOTHD010000683         1417 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_109138_length_1417_cov_3.905286, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000683 DAOTHD010000000
VERSION     DAOTHD010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109138_length_1417_cov_3.905286"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1366
                     /locus_tag="P5219_10410"
     CDS             <1..1366
                     /locus_tag="P5219_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRX27219.1"
                     /translation="NVFEDAQAHAPSIIFIDEIDAIAPKREDMGGEKQVERRVVAQLL
                     ALMDGLESRGQIVVIGATNIPNTLDPALRRPGRFDREISIPIPDRKGRHEILTIHTRG
                     MPLAADVDLKKVAEMTHGYVGADLEALAKEAAMASLRELLPEIDMEMEDIPYDHLLAM
                     DVRMDHFLEAFKEVEPSAIREVFVEVPDVTWRDIGGLDEIKEELRDAVQWPLQHGDIF
                     EKFDITPPKGIMLHGVSGTGKTLLAKALARESGVNFISVKGPSLMSKYVGESERAIRD
                     VFRTAKQASPSILYFDEIESLVPVRGKESGSGASFTERVISQFLAEMSGIEELKGVVV
                     LATTNRLDLVDPSLLSGGRFDLILELPRPDAQAREKIFEIHLRKKPLSSEVRIATLAR
                     LTDGRTGGDIAFVCRKAAMLAVRDHLKTGGDGELLMEWHHFESALRELKELPSVCRQN
                     EGTK"
BASE COUNT          322 a          408 c          424 g          263 t
ORIGIN      
        1 gaatgtcttc gaggatgccc aggcccatgc tccttccatc attttcatcg acgagatcga
       61 cgccatagcc ccgaaacggg aagacatggg cggcgaaaag caggtggaac ggcgggtcgt
      121 ggcccagctc ctggccctca tggacggcct cgagtccagg ggacagatcg tggtcatcgg
      181 tgcgacgaat atccccaaca ccctcgatcc ggccctccgg agaccaggac ggttcgacag
      241 ggaaatctcc atccccattc ccgacaggaa gggacggcat gaaatcctga ccatccacac
      301 ccgggggatg cccctggcgg ccgacgtgga tctcaagaag gtggccgaaa tgacccacgg
      361 ctacgtggga gccgacctgg aagccctcgc aaaggaagct gccatggcaa gcctcaggga
      421 acttcttccg gagatcgaca tggagatgga ggacatcccc tacgaccacc tccttgccat
      481 ggacgttcgg atggaccatt ttctcgaggc cttcaaggaa gttgagccct cggccatacg
      541 ggaagtcttc gttgaggttc ccgacgttac ctggagagat atcggaggcc tggacgagat
      601 caaggaagag cttcgtgacg cggtccagtg gcctctccag cacggtgaca tcttcgagaa
      661 gttcgacatc acgccgccca agggaatcat gctccacggc gtctccggaa cggggaaaac
      721 tcttttggcg aaggccctgg cccgggaaag cggagtgaat ttcatttccg tgaagggccc
      781 ctctctcatg tcaaaatacg tgggtgaaag cgaacgggcc atccgggacg ttttcaggac
      841 agctaagcag gcttcccctt ccattctgta ttttgacgag atcgagtcgc tggtgccggt
      901 gcggggcaag gaaagcggca gcggggcgag tttcacggaa cgggtgatca gccagtttct
      961 cgcggaaatg agcggcatcg aggagctcaa gggtgtggtg gttcttgcca cgacaaaccg
     1021 gctcgacctt gtggacccct ctctgctttc aggaggccgg ttcgatctga ttctcgaact
     1081 gccccgtcct gatgcccagg caagggagaa aattttcgaa atccacctgc gaaaaaaacc
     1141 cttgtcctcc gaggtccgca tcgcgacact ggcccggctt accgacggca gaacaggagg
     1201 cgatatcgcc ttcgtttgca ggaaggccgc catgctcgcc gtgagagacc atctgaaaac
     1261 gggaggagac ggggagctcc tcatggaatg gcaccacttc gaatcagccc tcagggaact
     1321 caaggagctg ccttctgtct gcaggcagaa cgagggaacc aaatagaaaa aaagaaggag
     1381 ccccgtgcta ttcctgcggg gcccctgttt tatttcc
//
LOCUS       DAOTHD010000684         1417 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_109229_length_1417_cov_1.785609, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000684 DAOTHD010000000
VERSION     DAOTHD010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109229_length_1417_cov_1.785609"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="P5219_10415"
     CDS             <1..556
                     /locus_tag="P5219_10415"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRX27220.1"
                     /translation="LMSKSVFPGKIGGDLQKHYSVLARELAGVHGRRILELGTGSGSA
                     VNFLHSDNEYVGTDISPGLLKRAAKRFLRAGFPAPEFYVASGDDLPFETGGFNLCLCI
                     LSLNFIGKAEKVFQEVHRLLISGGMFVCSVPVPERNRLRSTIRGVLHSEKELGGMCRE
                     SGFTFEPLPCDNGALLYFRATKQD"
     gene            661..1395
                     /locus_tag="P5219_10420"
     CDS             661..1395
                     /locus_tag="P5219_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRX27221.1"
                     /translation="MAAYTVLTITKDLGFTKSVIHPAAIRDESGIILFDAGYPDQADD
                     IEQALAEMGGTVADITAIFLSHHDHDHIGSLAELKRRNPKIRVVSGAKEADYISGKKQ
                     SLRLLQALEYNKTLSGEEKEFGERFARYLETVEHCPVDDVVQDLDYLCDGVRVIGTPG
                     HTPGHISLLLEEEKLLLAGDALAIEDGKLAKANPKFTLDAEEALKSVEKIKNLHLERI
                     SCYHGNMYTGPVEEELARILAEERNA"
BASE COUNT          336 a          406 c          414 g          261 t
ORIGIN      
        1 ccttatgagc aaatccgtct ttcccgggaa aatcggcggg gatctccaga agcactattc
       61 cgtcctcgcc cgggaattgg caggggttca cggcaggcgc atccttgaac tgggtacggg
      121 aagcggaagc gcggtcaact tcctccatag cgacaacgag tacgtgggaa cggatatcag
      181 ccccggactg ctgaaacggg cggcaaaacg gtttcttcga gccggcttcc cggccccgga
      241 attctacgtg gcaagcgggg acgacctgcc cttcgaaacc ggcggattta acctctgcct
      301 gtgcatcctg tcgctgaatt tcatcgggaa ggcggaaaag gtgtttcagg aagtccatcg
      361 cctcctcatt tccggcggca tgttcgtctg cagcgttccc gtcccggaaa gaaacaggct
      421 ccggagtacg atccggggcg tgctccattc ggaaaaagag ttgggcggaa tgtgccggga
      481 gagcggtttt acctttgaac cccttccctg cgataacggc gcgctcctgt atttcagggc
      541 aacgaaacaa gactgagaag tggagcggcc tgacgagggg ttccggggct cctctgcgga
      601 attgacctcg cccccggtaa gccgtgcgaa aaaataaaca ttacggagtt gagaattgaa
      661 atggctgcgt ataccgtcct gacgatcacg aaagatctcg gtttcacaaa atcagtcatc
      721 catcctgccg ccatcaggga tgaaagcggc atcatcctct ttgacgcagg ctacccggac
      781 caggcggacg acatagagca ggccctggcg gagatgggtg gtaccgtggc ggacataacc
      841 gccatcttcc tgtcccacca tgaccacgac cacatcggct cgctggcgga actcaagagg
      901 cgaaacccga agatcagggt tgtctcgggc gcgaaggaag cggactacat ttcgggaaag
      961 aaacaatcgc tgcggctgct ccaggccctg gagtacaaca aaaccctctc aggagaagaa
     1021 aaggaattcg gcgagcggtt cgcccgctac ctggagacgg tggaacactg ccctgtcgat
     1081 gacgtcgttc aggacctgga ctatctctgc gacggcgtga gggttatcgg gacccccgga
     1141 catacccccg ggcacatctc cctccttctt gaggaagaaa agcttctgct ggccggagac
     1201 gccctggcca tcgaggacgg aaaactcgcc aaagccaatc cgaagttcac ccttgatgca
     1261 gaagaagccc tgaaatccgt ggaaaagata aaaaatctgc acctcgagcg gatttcctgc
     1321 taccacggaa atatgtacac gggcccggtc gaagaagaac tggcccgcat cctggcggaa
     1381 gaacggaacg cctgacgccg ttctgctgcc gagtcgg
//
LOCUS       DAOTHD010000685         1414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_109525_length_1414_cov_2.738043, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000685 DAOTHD010000000
VERSION     DAOTHD010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109525_length_1414_cov_2.738043"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="P5219_10425"
     CDS             complement(<1..347)
                     /locus_tag="P5219_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27222.1"
                     /translation="MKPACLRRLEFRSILEMAAEGASTEAGRGRVMSLEPAGDRETSR
                     TLMLETLHASDLIGSSLEPALSSLGTAIETAGSLEDGSIALDPVLLRAAGSFMSDLID
                     FIGRADAAREEIPG"
     gene            complement(344..>1414)
                     /locus_tag="P5219_10430"
     CDS             complement(344..>1414)
                     /locus_tag="P5219_10430"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HRX27223.1"
                     /translation="EVLGVDRNASPEEIKKAYRAAAMKNHPDRNPGDKKAEERFKEAA
                     RAYEILSSPSRREAYDRFGTTGEPGSSGMGGMEDIFGGFGLDDALRAFMENFGFSGGG
                     SSRGTVRGDDVTVRVDLELEEVVLGGRREIQVERNSACEACGGTGADSEAGSESCRHC
                     GGAGRISTTRNTLLGSFRTVKECPVCRGAGRVPKKMCPGCRGRGYEKKKTSIAVDLPP
                     GLTRGHYIRLRGQGDHPGQGGTPGDLRLLVNDIDYGPFSREEDDLVFPLTVSYPQAVL
                     GADFEIPAPDGEPVSVQVPQGTPAGSRIRVRNRGIGRLDRRGRGDLLVEVGIHVPGKV
                     KRKEKELLKELEESPSFRNPGK"
BASE COUNT          237 a          486 c          390 g          301 t
ORIGIN      
        1 cctggaattt cttccctggc cgcgtcggcc cttccgatga agtcaatcag atcactcatg
       61 aaactccccg ctgcacgaag cagcacggga tccagggcta tggagccgtc ctcgaggctt
      121 ccggccgttt caatggcggt acccagcgaa gaaagagccg gttccagtga agaaccgatg
      181 agatccgatg cgtggagggt ctccagcatc agtgtcctgg aggtctccct gtccccagcc
      241 ggttccaggg acatgacgcg accccggcca gcctccgtgg aagccccctc ggccgccatt
      301 tccagtatgc tcctgaactc aaggcggcga agacaggccg gcttcatttc ccgggattcc
      361 tgaaggaagg ggattcctcc agttccttaa ggagctcctt ctctttcctt ttcaccttgc
      421 ccggaacatg gataccgacc tccaccagca ggtctccccg cccgcgtctg tccagacggc
      481 cgatgcccct gttccttacc cggatcctgc ttccagcagg cgttccctgg ggaacctgaa
      541 cggaaactgg ttcaccgtcg ggagcgggta tctcgaaatc ggctcccaga acagcctgtg
      601 gatatgaaac ggtaaggggg aagacaaggt cgtcctcctc cctggagaag ggcccgtagt
      661 ctatgtcgtt caccagaagc ctcaggtcac ctggagttcc gccctgtccg ggatggtccc
      721 cctggccccg aagccgtatg tagtgccccc tggtaagccc cgggggcagg tccacagcaa
      781 tggaggtctt cttcttctcg tagcccctgc ccctgcagcc ggggcacatc tttttaggga
      841 ccctgcccgc gcccctgcag accgggcatt ccttcaccgt tctgaagctt cccaggaggg
      901 tgttcctggt ggtggatatc cttccggctc cgccgcagtg cctgcagctc tcggacccgg
      961 cttcgctgtc ggctccggtc cctccacagg cttcgcaggc tgaattcctc tcaacctgta
     1021 tctcccgtcg cccgcccagg accacctctt ccagctcaag gtcaacacgt acggttacat
     1081 cgtcgcccct gacggttccc cttgaagagc cgccgccgga aaagccgaaa ttctccatga
     1141 acgccctgag ggcgtcgtcg aggccaaagc ctccgaatat gtcctccatg ccccccattc
     1201 ccgaggaacc gggttccccg gttgtcccga aacggtcata ggcttccctc cgggaggggc
     1261 tgctgaggat ctcgtaggcc cttgcggcct ccttgaaacg ctcttcggcc tttttgtcgc
     1321 cggggttgcg gtcgggatgg ttcttcatcg ccgcggcccg gtaggccttc ttgatctctt
     1381 cgggggacgc gttcctgtcc acacccagta cttc
//
LOCUS       DAOTHD010000686         1412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_109744_length_1412_cov_2.574060, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000686 DAOTHD010000000
VERSION     DAOTHD010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109744_length_1412_cov_2.574060"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="P5219_10435"
     CDS             <1..835
                     /locus_tag="P5219_10435"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRX27224.1"
                     /translation="GYHCSPGRMYVPATDLYRGLRYGVLATTFGCPFRCEYCASRLLE
                     PSYRRRDPGLVLEDAARQLSGGGAKDLAFYDDALLVEKERHFYPLLEALNKRHPGTRF
                     HSPNGLHVREIDDECAEMLKKRGFETLRLSFESSDPEMQRRGSDKTSDEEYVAALASL
                     RKAGFSGGQLETYILAGVPGQSVESVERSIRFVLEHGGNPKIAEFSPVPGTPSFRRAA
                     ELLPGLTDEPLLSNKTVYSSYLAGNMSPPELQALKDLAKGQREGRSKKPKFAKRTAYY
                     P"
     gene            896..>1412
                     /locus_tag="P5219_10440"
     CDS             896..>1412
                     /locus_tag="P5219_10440"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRX27225.1"
                     /translation="MKDIPEQFTKQIRSFNRYYTGVIGAVNQSVLNSPFSLAEARVLY
                     EIKHNESPTASVINVQLGLDPGYLSRIIRRFEHDELLEKERSPLDGRAYILRLTPRGE
                     HVLTALEKASDQRASELLTPMPKTDVEALLRSMETIRGILSNDPRDVTVRTAKPGELG
                     IVASQHMDFYMK"
BASE COUNT          332 a          458 c          385 g          237 t
ORIGIN      
        1 tggatatcac tgcagcccgg gccggatgta cgtgccggcc accgacctct accggggact
       61 gcggtacggc gtcctcgcca ccaccttcgg ctgccccttc cggtgcgaat actgcgcctc
      121 ccgccttctc gaaccgtcct accggagaag ggatcccggt ctcgtcctgg aggatgcggc
      181 ccggcagctc tcgggcggcg gggcaaagga cctcgccttc tacgacgacg ccctcctcgt
      241 ggaaaaggag cggcattttt atcccctcct tgaggcgctg aacaaacggc accccggaac
      301 caggttccac agccccaacg gactccacgt ccgggaaatc gacgacgaat gcgccgaaat
      361 gctgaaaaaa agaggcttcg agaccctccg cctcagcttc gagagctccg accccgaaat
      421 gcagcgcagg gggtcggaca aaacatcgga cgaggaatac gtggcggccc tggcatctct
      481 cagaaaggca ggattttccg gcgggcagct tgagacctac atcctcgcgg gagtaccggg
      541 acagtctgtc gagtcggtgg aaagatccat ccggttcgtc ctggagcacg gaggaaatcc
      601 caaaatagcc gagttttcgc ccgttcccgg aacaccctcc ttcaggcggg cggccgaact
      661 gcttcccggc ctgactgacg aacccctgct ctccaacaag acggtctact cctcctatct
      721 tgccggtaat atgtcacctc cggaactcca ggcactcaag gatctggcga aaggccagag
      781 ggaagggcgg agcaaaaagc ccaaatttgc aaaaagaact gcatattatc cttgacatca
      841 gcaataaatt ggaatactat aaggagaaag agtcatcgaa aggagggcaa aacaagtgaa
      901 ggacattcct gaacagttta cgaaacagat ccgcagtttc aaccgatact acaccggagt
      961 cataggagcg gtcaaccagt cggtgctcaa cagccccttc tccctcgccg aggcgcgggt
     1021 gctctacgag atcaagcaca acgagtcccc cacggccagc gtcatcaacg tccagctcgg
     1081 ccttgatccg gggtacctca gccggatcat ccgccggttc gagcatgacg aacttctcga
     1141 aaaggaacgg tctcccctgg acggcagggc ctacatcctc cgcctcaccc cccggggaga
     1201 acatgttctc accgccctcg aaaaagcgtc ggaccagagg gcgtcggaac tgctgacccc
     1261 catgcccaag actgacgtgg aagccctcct gaggtccatg gagaccatcc gcggcattct
     1321 gtccaacgat cccagggacg tgaccgtccg cacggcaaag cccggagaac tcggcatcgt
     1381 cgcctcccag cacatggact tctacatgaa gg
//
LOCUS       DAOTHD010000687         1412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_109746_length_1412_cov_2.557111, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000687 DAOTHD010000000
VERSION     DAOTHD010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109746_length_1412_cov_2.557111"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..246
                     /locus_tag="P5219_10445"
     CDS             4..246
                     /locus_tag="P5219_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27226.1"
                     /translation="MIVGAMVVGAVDSIRPFGEPGASPMDDYFIASALKDRSSENVVT
                     SIVFDYRGFDTIGEAAVLFTALCAITALFREGRKKL"
     gene            243..725
                     /locus_tag="P5219_10450"
     CDS             243..725
                     /locus_tag="P5219_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HRX27227.1"
                     /translation="MRPLSVVVRTGCDLFAWFLIVFGANVIIHGDVTPGGGFQGGAVA
                     ATFITLLLVAYGGKKVFSWVRPGIFNGMLIFGLMAFFLLGLMGFPNSFFYNFIAVPHE
                     VVQATGHGVIPPSGTIALMDIAVGIEVAGALSIIVLSMFRGIRLFREGPMEEESGHDR
                     "
     gene            715..1062
                     /locus_tag="P5219_10455"
     CDS             715..1062
                     /locus_tag="P5219_10455"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HRX27228.1"
                     /translation="MIGNAPFFVVGVLFLMGLVSLFLSKNLIKIAIAISIIGSAVNIF
                     LVSLGYRAGGTIPVHFLAGEGSTMVLPTPQAMTLTAIVIALATTALLLSLIMLVWRHY
                     GTLDIDEIRRLRG"
     gene            1059..>1412
                     /locus_tag="P5219_10460"
     CDS             1059..>1412
                     /locus_tag="P5219_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HRX27229.1"
                     /translation="MNWNEHFPALLLIIPLFGAFLAPVCSLGGKMARNILLVAASFLT
                     LVVAFLLWRSVLANGIQYYVMGGENFRLALPSGMALPVRIILEVDAFSAFMALCGSIA
                     AFAGALFSVNYMNRFS"
BASE COUNT          244 a          407 c          393 g          368 t
ORIGIN      
        1 gcgattatcg tcggagccat ggtcgtgggg gcggttgact ccatacgccc ctttggagag
       61 cccggtgcct cacccatgga tgactacttc atcgcaagcg ccctgaagga ccggtcatcg
      121 gagaatgtgg tgacctccat cgttttcgac tacagggggt tcgacaccat aggagaggca
      181 gcggtcctct tcacggctct ttgcgccatc accgccctct tccgtgaagg gaggaaaaag
      241 ctgtgaggcc gctttctgtc gtcgtcagaa cgggctgtga tcttttcgcc tggttcctga
      301 tcgtctttgg cgcgaatgtc atcatccacg gagatgttac cccgggagga ggcttccagg
      361 gaggggcagt cgccgcaacc ttcatcaccc ttcttcttgt cgcctacggc gggaaaaaag
      421 tcttttcctg ggttaggccc ggcatcttca acggcatgct cattttcgga ctgatggcgt
      481 ttttcctgct cggcctcatg ggattcccga actcgttctt ttacaacttc atcgccgttc
      541 ctcacgaagt tgttcaggcg acgggccacg gtgttatccc tccgtcggga accatcgccc
      601 ttatggatat cgccgtcggc atcgaggtcg cgggagctct ttccattatc gtgctttcca
      661 tgttcagagg catccggctt ttccgtgagg ggcccatgga ggaggaatca ggccatgatc
      721 ggtaacgcac cgtttttcgt cgtaggagtc ctgttcctca tgggactcgt gagccttttc
      781 ctttcgaaaa atctcattaa gatcgccatt gcgattagca tcatcggaag tgccgtcaat
      841 atttttcttg tatctctggg gtacagggcg ggggggacca ttccggtcca ttttctcgct
      901 ggtgagggca gcaccatggt gcttcccacg ccccaggcga tgaccctgac cgccattgtc
      961 atcgccctgg cgaccacagc ccttctgctg tcgcttatca tgctcgtctg gcgtcattat
     1021 ggaacccttg acatagacga gatccggagg ttgagaggat gaactggaac gaacattttc
     1081 ccgcgctgct tctgataatt cctctcttcg gcgcattcct cgcgccggtc tgctccctgg
     1141 gaggaaagat ggccaggaat atcctgctgg tcgcggcgtc cttcctcacc ctggtagttg
     1201 cgttcctgct ctggcggagt gttctcgcca acgggataca gtactatgtc atgggcggcg
     1261 agaatttcag gctcgccctg ccgtcgggaa tggcccttcc cgtgaggatc attctcgaag
     1321 tggatgcttt cagcgccttc atggccctgt gcggatccat cgcagctttt gccggagcgc
     1381 tcttctcggt gaactacatg aacaggttct ca
//
LOCUS       DAOTHD010000688         1411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_109850_length_1411_cov_2.770649, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000688 DAOTHD010000000
VERSION     DAOTHD010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109850_length_1411_cov_2.770649"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..>1411)
                     /locus_tag="P5219_10465"
     CDS             complement(57..>1411)
                     /locus_tag="P5219_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27230.1"
                     /translation="VDGTVTVKGPLSNPAVTVKASSPKVSLQGMAVTDVALGLSGNTK
                     EMKIEEFRSKFGGGTLGATGNVKLGAAPDVTVDISGKDLDLAALTAGMPDAKELRIGG
                     KVNASFKGRFAGASGKGEGSITSQSLTVMGLKASALNYPLVLEGNKLSGKGGSAGFYG
                     GKVSGSGSLDISTMKFSHSAELSGVDVNGVLQDFTGGLGGKVTGLAQGSANVSGTLAP
                     KFALSGKGSAKIGEGAVSGFTGLDIVTKLHGVSGIRYTEVTAPFRLETGRIILEKGTK
                     ATAPKNDPLYRFLTAEGPVGPKGALKLLVAGNVNIQIINALAGGALGGLTAGSLEDAL
                     KGVLGGAQKGMEKADFRDISLSVGGTVEKPSVSNLKVAPGAQQPAAPAQKPAEQPQAP
                     KTPEQAILEKIIKPAEKPAPAPVPAPAPTPAPAPKPKPEEPKKPEDILKEKLLESIFK
                     "
BASE COUNT          221 a          432 c          452 g          306 t
ORIGIN      
        1 cagggggtgt gcaggaaagg gggaggcttt cgcctccccc ggtggtctgt tgtctattac
       61 ttgaagatgg attcgaggag tttttccttc aggatgtcct ccggtttttt cggctcctcg
      121 ggcttgggct ttggggccgg ggccggagtg ggagcgggag cgggcacggg agcaggagcc
      181 ggtttttccg caggcttgat tatcttttca agaatggcct gttcgggagt cttcggcgcc
      241 tggggctgtt ccgccggttt ctgggccggg gcggcgggct gctgcgcacc cggggccacc
      301 ttcaggtttg aaacggaagg cttctccacg gtaccgccca cggacaggga gatatcccgg
      361 aagtccgcct tttccattcc tttctgggca cctccgagaa cacccttcag ggcatcctcc
      421 agggatcccg ccgtcaggcc gcccaaggct ccgccggcaa gggcgttgat gatctggata
      481 ttcacgttcc ccgccaccag gagcttgagg gctcccttgg ggccgacggg tccctccgcg
      541 gtaaggaacc tgtaaagggg gtcgttcttg ggtgccgtcg ccttcgttcc cttttcgaga
      601 atgatgcgtc ccgtttcgag acggaaggga gcggtgacct ccgtgtatct gatcccgctg
      661 acgccgtgga gcttcgtcac gatatccagt cccgtgaacc cggagacggc cccttcgccg
      721 atcttggcgc tccccttgcc cgacagggcg aacttggggg cgagggttcc ggagacgttg
      781 gccgatccct gggcaagacc ggtgactttt ccgccgagac ctccggtgaa gtcctggagg
      841 actccgttga cgtcaacgcc gctgagttcg gcgctgtggg agaacttcat ggtcgaaatg
      901 tccagggagc cgctgccgga gaccttcccg ccgtagaatc cggcgcttcc tcccttaccg
      961 gaaagcttgt ttccctcgag gaccagggga taattcagtg cagaggcctt gaggcccatg
     1021 accgtcaggg actgggatgt gatggatcct tcgcccttgc cggaggcgcc ggcgaaccgc
     1081 cccttgaagg aggcgttcac cttgcccccg atgcggagtt ccttggcatc gggcattccg
     1141 gcggtgaggg cagcgaggtc gaggtccttc cccgagatat ccaccgtcac atcgggagcc
     1201 gctcccagct tgacgtttcc cgtcgctccg agagtgcctc ccccgaactt tgaacggaac
     1261 tcctctatct tcatttcctt cgtatttccg ctgagcccaa gggcaacatc ggtaacggcc
     1321 attccctgaa gagagacctt cggcgaggat gccttgaccg tcacggccgg gttcgacaag
     1381 ggccctttga cggtgaccgt gccgtccacc a
//
LOCUS       DAOTHD010000689         1410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_110017_length_1410_cov_1.936531, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000689 DAOTHD010000000
VERSION     DAOTHD010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110017_length_1410_cov_1.936531"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="P5219_10470"
     CDS             <1..849
                     /locus_tag="P5219_10470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRX27231.1"
                     /translation="ASVRAASARTSETGARKEYAQAQSKRYDNLLKSGAVSVDAAEAK
                     RQERLVTAAAWASANADLQASRAELERAKADLAALVSQKENLDLLAPAGGLVSARAAE
                     PGTTAVAGQAVVEIIDTGSIRVSVRFDQAAASGLRAGLPADIVLRSGNGRVHRGAILR
                     VEPLADPVTEEMIAKAVFGEIPDPLPPLGELAEVTVHLPPLPGTPVVPDGALQRYNGR
                     PGVWVIENGSIAFRPVKTGRRDLAGTVQILEGLEEGERVVVYSARALTPKSRVKVLET
                     LEGMKP"
     gene            846..>1410
                     /locus_tag="P5219_10475"
     CDS             846..>1410
                     /locus_tag="P5219_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRX27232.1"
                     /translation="MISLAGRDILHGWGKFALTGLGLGLLIGVTLTMGGVYRGMVDDA
                     MVLLDNSGADLWVVQQETLGPYAESSSIFDDTWRSVSGMPGVEEAANVTYLTTQVRRG
                     TEDVRAMVVGVDPGMPGSPGQPGYLTAGRHITRSHYEAVADGATGFRPGDEIRIRRNT
                     YTVVGLTRRMVSSGGDPMIFIPLKDAQE"
BASE COUNT          253 a          416 c          508 g          233 t
ORIGIN      
        1 gcttctgtgc gggctgcttc cgccaggacg tcggagaccg gcgcgaggaa ggagtacgcc
       61 caggcccagt caaagcggta cgacaacctc ctgaaatcgg gggcggtgag cgtggacgcc
      121 gccgaggcga agcgccagga gcgcctggtc acggcggctg catgggcctc ggccaatgcg
      181 gatctccagg cctcccgggc ggagctggag cgggcgaagg ccgatctcgc ggcgctcgtg
      241 tcccagaagg aaaatctcga tctcctggct cctgccggag ggctggtgtc ggcccgggcg
      301 gccgaaccgg gtacgacggc ggtggcgggc caggccgtgg ttgaaataat agacaccggg
      361 agcatcaggg tttcggtccg ctttgaccag gccgccgcct cggggctccg ggccggtctt
      421 cctgcggata tcgtgctccg gtcagggaac ggtcgggtgc acaggggagc catcctgcgg
      481 gtggagcccc tggcggatcc tgttaccgag gaaatgatcg ccaaggcggt cttcggcgaa
      541 ataccggacc ctcttcctcc cctcggggag cttgcggagg tgaccgtaca tcttcctcct
      601 ctccccggaa cgccggtcgt ccccgacggt gccctgcagc ggtacaacgg caggccgggg
      661 gtgtgggtga tcgagaatgg ttccattgcc ttccggcctg taaagacggg acggcgtgac
      721 ctggccggaa cggtgcagat ccttgaagga ctcgaagagg gcgaacgggt ggtggtgtac
      781 agcgccaggg ccctgacgcc gaagagcagg gtgaaggttc tcgaaaccct ggaggggatg
      841 aaaccatgat cagcctcgcg ggcagggaca ttctccacgg atgggggaaa tttgccctca
      901 ccggcctggg gctggggctt ctcatcggag tgaccctcac catgggcggc gtttaccggg
      961 gcatggtgga cgatgccatg gtgctcctcg acaacagcgg cgccgatctc tgggtcgtcc
     1021 agcaggagac cctgggtccc tacgcggagt cgtcaagcat tttcgatgac acctggagga
     1081 gtgtctcagg aatgccgggt gttgaggaag cggcaaacgt aacatatctt acgacccagg
     1141 tccggagggg cacggaagac gtccgggcca tggtggtggg ggtggacccc ggcatgccgg
     1201 gatctccggg acaaccgggg tacctgacgg ccggacggca tatcacccgc agccactatg
     1261 aagcggtagc cgacggggcc acgggatttc gtcccggtga cgaaatccgc ataaggagga
     1321 atacctacac ggtggtgggc cttacgcgcc gcatggtctc ctccggaggg gaccccatga
     1381 ttttcatccc cctgaaggat gcccaggagg
//
LOCUS       DAOTHD010000690         1409 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_110062_length_1409_cov_3.337518, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000690 DAOTHD010000000
VERSION     DAOTHD010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110062_length_1409_cov_3.337518"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1405
                     /locus_tag="P5219_10480"
     CDS             <1..1405
                     /locus_tag="P5219_10480"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRX27233.1"
                     /translation="PLASRNGRKARYYRAFSLYSLKKYGEALELWSYLARKGTSYAES
                     SIRRISILAGRAQKDRALRVLREVAASREGAIKARALYSLSTHASGKEKRELEEKVIA
                     LIPDSAFTTRILYNRGWDRWNSGDIKGAVQIWEKSIASGMDGNWRPRVLYWLAKGYGR
                     LKLQEKRAAALEKIRKNHPLSIYAFLSGGDKLSIVPDVPRHLVQDSPSDLERWGFITY
                     ARWNLLARGDAKSLFRASRLAEWSGDHLAAYSAMGRIAGEITRGPSFFRKGMEFLYPR
                     PFLEEVKKAAERFKVEENLVWAIMRQESAFNPNATSWVGAAGLMQLMPSTARSEAKAL
                     KMKDYNIYNAGQNIVLGTAHIARLLKSFGNVEQSVAAYNGGGGSARRWLAGRKDVPLD
                     EWIESVRFEETNDYVRKVMANLHIYRALYGTSGSGGTVPAAPGEVPGDDLESSADGPD
                     PEDEDNEAPLLLDDGGK"
BASE COUNT          340 a          393 c          430 g          246 t
ORIGIN      
        1 tcccctcgcg tcccgcaacg gaagaaaggc gagatactac cgtgcctttt ccctctatag
       61 cctgaagaaa tacggtgaag ccctcgagct ctggagttac ctggcgcgga aaggcacctc
      121 ctatgccgaa tcctccatac ggagaatttc catcctcgca ggaagagccc agaaggacag
      181 ggccctcaga gtgctcaggg aagttgcggc ctcccgggag ggagcgataa aggcgagagc
      241 cctgtattcc ctctcgaccc acgcttcggg aaaggaaaaa cgggaactcg aggaaaaggt
      301 catcgccctc ataccggatt cggccttcac cacccgtatc ctctacaacc gtggatggga
      361 ccggtggaac agcggggaca tcaagggtgc ggtgcagata tgggaaaaga gcatcgcctc
      421 aggaatggac ggaaactggc gtccaagggt tctctactgg ctcgcaaagg ggtatggacg
      481 gctgaagctg caggaaaaga gggcagcggc tcttgaaaag atacggaaaa accatcccct
      541 ttccatctac gcttttctca gcggagggga caagctgtcc atcgttcctg acgttccccg
      601 ccacctggtg caggacagcc cctcggacct tgaacggtgg gggttcatca cctatgcgag
      661 gtggaatctg ctcgcaaggg gcgacgccaa atccctcttc cgcgcatccc ggctggcgga
      721 gtggagcggt gaccacctgg ccgcatattc ggcgatgggc cgcatagccg gagagatcac
      781 ccgggggcct tcgttcttca ggaagggaat ggagtttctc tatccccggc cctttctgga
      841 agaggtcaaa aaggctgcgg aacggttcaa ggtggaggaa aacctggtat gggccatcat
      901 gcggcaggaa agcgccttca atcccaatgc cacaagctgg gtgggcgccg ccgggctcat
      961 gcagctcatg ccctccacgg cacggagcga agcgaaagca ctgaaaatga aggattacaa
     1021 tatctacaac gccggacaga acatcgttct cggaacggcc catatagccc gtctcctgaa
     1081 atccttcggg aacgtggagc agtccgtggc tgcgtacaac ggaggaggcg gctcggcaag
     1141 acgctggctc gccggaagga aggacgtacc cctggatgaa tggattgaat cggtccggtt
     1201 cgaggaaacc aacgactatg tcagaaaagt gatggccaac cttcacattt acagggcgct
     1261 gtacggaact tcgggatcgg gaggaacggt tccggccgcc cctggagagg ttcccggaga
     1321 cgaccttgaa tcgtccgccg atggcccgga tccggaggac gaagataatg aagcccctct
     1381 tctgctggac gacggaggga aataaaaca
//
LOCUS       DAOTHD010000691         1409 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_110065_length_1409_cov_3.323486, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000691 DAOTHD010000000
VERSION     DAOTHD010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110065_length_1409_cov_3.323486"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /gene="dapA"
                     /locus_tag="P5219_10485"
     CDS             complement(<1..678)
                     /gene="dapA"
                     /locus_tag="P5219_10485"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRX27234.1"
                     /translation="MFGGTGTAVVTPFKEGGVDFESFGRFLNWQIEGGVEFLVVLGTT
                     GESPTVTADERAEIITFAVRTAAGRVPVVIGTGSNSTAGAVALSQQAEALGADGVLVV
                     TPYYNKPTQEGLYQHFKAVAAAIKIPLIVYNVPGRTGTNILPETVHRLSRLLNVAGIK
                     EAAGDVAQIDTLVRLIKKDRPDFAILSGNDDQAFHLVNSGGDGVISVLSNVMPRETSD
                     MIRLALAG"
     gene            complement(1103..>1409)
                     /locus_tag="P5219_10490"
     CDS             complement(1103..>1409)
                     /locus_tag="P5219_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HRX27235.1"
                     /translation="PASRPATVNRLDEIGAARRPWVGAVSCNPAEQARLLDEAGSQFS
                     IVLGLCVGHDSIFYRHSRAPVTTLVVKDRVLGHNPAAVLYSQYLRKNLKRAPGERTE"
BASE COUNT          263 a          466 c          400 g          280 t
ORIGIN      
        1 cccggcgagg gcaaggcgga tcatgtccga cgtctcccgg ggcatgacgt tggacagcac
       61 ggaaatgact ccgtcgccgc cggaattcac gagatggaaa gcctggtcgt cgttcccgga
      121 gaggatggcg aaatcgggac ggtctttctt tataagccgg accagggtgt cgatctgggc
      181 aacgtctccg gccgcctcct ttattcccgc cacgttgagc agtctgctca gccggtggac
      241 cgtctcggga aggatgttcg tccccgtgcg gcccgggacg ttgtagacga tgagagggat
      301 ctttatcgcc gccgcgactg ctttgaaatg ctggtacagc ccttcctggg tgggcttgtt
      361 gtaatacggg gtcaccacga gcacgccgtc ggcccccagg gcctcggcct gctgggacag
      421 ggcgaccgca cccgccgtgg aattgcttcc cgtcccgatg accaccggaa ctcttcccgc
      481 cgccgtcctc acggcgaagg tgatgatctc cgcccgctcg tcggcggtaa cggtggggct
      541 ttctcccgtg gtccccagga ccacgaggaa ctcaacaccg ccctcgatct gccagttcag
      601 gaaacgcccg aaactctcga aatccacgcc gccctcttta aacggggtca ccactgcagt
      661 acctgttcca ccaaacatct ccgcacctcc tgttgttgtc gggatagaaa aaacggcccg
      721 cccccgaagg gacgagccgt aactcgcggt accaccctaa ttggacatgg aagaaattca
      781 tgtccctctc gggcccgatg acgggagccc acaccgggaa tgtactccgg aaacctcctg
      841 tttttaggag atcctttcca ttccggctca ccggcctttt cgcccttttc ccccggcggg
      901 gacgcctctc agccgctgaa cgcccctctc tgtcgccgaa cgaccggaaa agaacatacc
      961 ggttcatcgc cttttcagat atatttggag attttatgca aagcggctct ttttgtcaat
     1021 agaaaaatca ctgaatgccc ccggggacat tcccttctcc ggaaatttcc ggaaaagagg
     1081 atccgcggcc tgggggctgc attcattcag tgcgctcccc gggagcccgc ttcaggttct
     1141 tcctgagata ctggctgtag agcaccgccg cggggttgtg ccccagcacc ctgtccttga
     1201 ccaccagggt cgtgaccgga gccctggaat ggcggtaaaa aatgctgtca tggcccacgc
     1261 acagcccgag aacgatgctg aattgagacc ccgcctcgtc gagcagccgg gcctgttccg
     1321 cggggttgca cgagactgcc cccacccagg ggcgcctcgc cgcgccgatc tcgtccaacc
     1381 tgttcaccgt cgccgggcga ctcgccgga
//
LOCUS       DAOTHD010000692         1409 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_110083_length_1409_cov_2.665436, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000692 DAOTHD010000000
VERSION     DAOTHD010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110083_length_1409_cov_2.665436"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..615)
                     /locus_tag="P5219_10495"
     CDS             complement(7..615)
                     /locus_tag="P5219_10495"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HRX27236.1"
                     /translation="MKASWNGAREWKDRIAGVFSPPADPPWEKVTADGPGKSAVLVPF
                     FPGPDGPELLFLRRSATLRHHAGEICFPGGMREPGDFGPVSTALRETAEETGIRPECV
                     ETLGILEPEYAVVSGVAVFPVVGLVSRFKPADLDLSSGETEGACFAGVDRFSDVPVTK
                     TVEVRGTLHEYPEYRLENGWIIWGVTARILRRVLGSLKGGMC"
     gene            complement(612..>1409)
                     /locus_tag="P5219_10500"
     CDS             complement(612..>1409)
                     /locus_tag="P5219_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HRX27237.1"
                     /translation="LRSVETMKRIVARAESEIELWERPLHSTSKTMGVPDAVSSASVL
                     VARQMKAAAIISMTQSGSTAQMVSKHRPPCPILGATPSVRTWRELALYWGVIPLMKEE
                     MTDQNTAVDSAIASCISAGLIREGDLVVVTAGVPLGAAGTTNMLQVHIAGTILLKGLS
                     LLKREARGPVCIAGSAAEANEKMTRGAVLVVGATDREYVPAMKLASAIVAEEGGLTSH
                     AAIVSLELGIPCVVNAEKALSLLSDGMIVTVDGYRGMIYHGRVKLTV"
BASE COUNT          261 a          461 c          425 g          262 t
ORIGIN      
        1 gggccatcag cacatcccgc ctttcagact cccgagcacg cggcggagta tccgggcggt
       61 aacgccccaa ataatccagc cgttttcgag gcggtattcc gggtattcat gcagtgttcc
      121 ccggacttcg acggtcttcg tcacgggaac gtccgaaaag cggtcaacgc ctgcgaagca
      181 ggcgccctcg gtctctcctg aggagaggtc gagatcggcg ggcttgaacc gggaaaccag
      241 acctaccacg ggaaacactg ccacacctga aacgacggca tattccggtt ccaggatgcc
      301 gagggtttcc acacactccg ggcggatgcc cgtttcctcc gcagtttccc tgagagccgt
      361 ggaaaccggg ccgaaatccc ccggctctct catgcccccg ggaaaacaga tctcccccgc
      421 atgatgcctg agtgtggcag accggcggag aaaaaggagc tccggcccgt cgggtcccgg
      481 aaagaacggg acaagcacag cgctctttcc cgggccgtca gcagtcactt tctcccacgg
      541 aggatccgcg ggaggtgaga aaaccccggc gatgcggtct ttccattccc gggcaccgtt
      601 ccacgaggcc ttcatacggt cagtttcacc cggccgtggt agatcatccc ccggtagccg
      661 tcaacagtaa cgatcatgcc gtcagaaagc agggaaaggg ctttttcggc gttcaccacg
      721 cagggtatgc cgagctccag ggaaacgatg gcggcgtggg acgtgagccc tccctcctcc
      781 gccacgatgg ccgaagccag cttcatggcc ggaacatact ctctgtccgt cgcccccacg
      841 acgagaacgg ctcccctggt cattttttca ttggcctctg cggcactccc ggcgatgcac
      901 acgggccccc tggcctcccg cttcaggagg gaaaggccct tgagaagaat tgtgcccgca
      961 atgtggactt ggagcatgtt cgtggtgcct gcggcaccca ggggcactcc cgccgtcacc
     1021 acaacgaggt cgccctccct gatcagtccg gcgctgatgc aggaggcgat tgctgagtcc
     1081 actgccgtgt tctggtcggt catctcctct ttcatgagag ggatgacacc ccagtacagg
     1141 gcaagttccc gccaggtcct gacggatgga gtcgccccga ggatggggca cggaggcctg
     1201 tgcttgctca ccatctgggc ggtgctgccg ctctgggtca tggagatgat ggcggcagcc
     1261 ttcatctggc gggccaccag gacggaggcg ctgctcacgg catccgggac acccatggtt
     1321 tttgatgtgc tgtgcaacgg gcgctcccag agctctattt cgctttccgc ccgggccacg
     1381 atgcgcttca tggtctccac cgaccggag
//
LOCUS       DAOTHD010000693         1407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_110302_length_1407_cov_2.717456, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000693 DAOTHD010000000
VERSION     DAOTHD010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110302_length_1407_cov_2.717456"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="P5219_10505"
     CDS             complement(<1..448)
                     /locus_tag="P5219_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX27238.1"
                     /translation="MLEDLSHHILDIAENGVNAGADEISISIDDSGDMLLLSVRDNGR
                     GMTEETVKAVVNPFYTTRTERRVGLGLPFLKQLAELCGGVFSVSSEPGRGTQVTASFL
                     KTSIDVPPLGDVPSTLMTLFIGYPGIAWKYIHRRNEKEFSADSVELR"
     gene            complement(451..930)
                     /gene="nuoE"
                     /locus_tag="P5219_10510"
     CDS             complement(451..930)
                     /gene="nuoE"
                     /locus_tag="P5219_10510"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRX27239.1"
                     /translation="MVVGTQQDARSLVREITAPWKGKQGGVIPVLQETQKQIGYLSRE
                     AMEAISEELSVPLAELYGVATFYAQFHLKPRGRHIIRVCRGTACHVRGSAKIMERIMS
                     MLGIEENGTTEDLRFTLEPVACLGCCGLAPVVMIDEDTHGRLTDDPAKLQAFLDNYS"
     gene            complement(1192..>1407)
                     /locus_tag="P5219_10515"
     CDS             complement(1192..>1407)
                     /locus_tag="P5219_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27240.1"
                     /translation="TILLGPTASLFPGETADKMLILADALEIADHTEEAMVKAESRED
                     WGVLSREERIAAALKILSEGSYLPLTD"
BASE COUNT          324 a          380 c          348 g          355 t
ORIGIN      
        1 ctctgagttc cacgctgtcc gcagagaatt ccttttcgtt ccgcctgtgg atatatttcc
       61 acgcaatgcc gggataacct atgaataagg tcatgagcgt agagggaacg tcaccgagag
      121 gcggcacatc gattgaagtc ttcaaaaatg aggccgtcac ctgcgttccc cttccgggtt
      181 cggaggaaac ggaaaaaact cctccgcaca gttccgcaag ctgtttgaga aaaggaagac
      241 caagtcctac ccttcgttcc gtcctcgttg tgtagaaagg gttcactacg gctttgacag
      301 tttcttccgt cattccccgt ccattgtctc tgacggacag aagaagcatg tcaccggaat
      361 catcgatgga aatggaaatt tcatcggctc cggcgttcac gccgttttcc gcgatatcga
      421 ggatgtggtg ggaaaggtcc tcgagcatga tcaggaataa ttgtcgagga atgcctgaag
      481 tttcgcagga tcgtctgtaa gccttccgtg agtgtcctcg tcgatcatca cgaccggagc
      541 caaaccgcag catccaaggc aggcgacagg ttcgagggtg aagcgaaggt cctcggttgt
      601 tccgttctcc tcgatgccga gcatgctcat gattctttcc attattttgg cgctcccccg
      661 tacgtggcag gcggttcccc tgcagaccct gatgatatgt cttcccctgg gcttcagatg
      721 gaactgggcg taaaacgttg ctactccata cagttccgca agaggcaccg aaagttcttc
      781 ggatatggcc tccatagcct cgcgggaaag gtaccctatc tgcttttggg tctcctgcag
      841 aacaggtatg acgccgccct gctttccttt ccacggcgcc gttatttccc gcaccaaaga
      901 ccgcgcgtcc tgctgtgttc cgacaaccat tgcccgacct ccactcttat gtgaaattta
      961 taacattata aatacaaaac tacctgcctt tatttgtttt ccctgccagg catggcctga
     1021 acgggaaaat ttcacggggg aaactcattt tttgttcatg tttaaaaaaa gtgataaaaa
     1081 atcaaccttt tccacacgca taggatatca tcacggcttt tttccgacaa attttgaata
     1141 gcgaaagccg caatttaaaa aagcacaccc tgaatatccc ccccagcggt atcagtcggt
     1201 cagcgggaga tatgagccct cggaaaggat tttcagcgcg gcggcgatac gttcttccct
     1261 tgaaagaact ccccagtctt ccctgctttc ggccttgacc atggcttctt cggtatggtc
     1321 ggcgatctcg agggcatcgg cgaggataag cattttgtcc gccgtttccc cggggaaaag
     1381 ggacgctgtg ggccccagga ggatagt
//
LOCUS       DAOTHD010000694         1403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_110684_length_1403_cov_4.149852, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000694 DAOTHD010000000
VERSION     DAOTHD010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110684_length_1403_cov_4.149852"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            420..>1403
                     /locus_tag="P5219_10520"
     CDS             420..>1403
                     /locus_tag="P5219_10520"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HRX27241.1"
                     /translation="MFTIKEFLRTEVKPALGCTEPGAVALAVARAKEELGGPVDAVSV
                     TVSDSIYKNGVDVGIPGTEGLRGNSVAAALSAFCGRSAYGLEVLKDCTEDHIASAKAM
                     LGKNAVQINPDLDRHGVYVEASVRSGEDRAVCRIEGSHTGISEVTLNGKTTFSAEKTP
                     SSAPNGPEANVSDEIARMSFPDLVSLVETMDGEDMDYVMSGVEMNMTIAEYGFDTSHP
                     TGLDIGKTLKKLSGAAFNSEDLSLRIKAYAAAASDARMAGAPLAVMSSAGSGNHGVTA
                     ILPVAVCAQHHGKSREETARAVAFSHLATSYIKSRTGRLTPTCGCTVAAGAG"
BASE COUNT          329 a          442 c          397 g          235 t
ORIGIN      
        1 ccagtccgac gctggcggta gttcccactc cgccgcatcg ggcaacatgc ccggcaaggt
       61 tcggcccgga aatggcgacc cccatccctc cctggatcag cggatacagg ggctggtgtt
      121 tccctatctt caataccgga agttgactca aagggctctc tcctcttcaa aatgaactgc
      181 cggcgctccc ggggcttgcc atcccggagg gaccggtgcg gacggtgcgc cgtaccgtgc
      241 tattctatcc cacattcaag cattcaatca aggaagaaat actgaaggtc aaaaaaacgg
      301 ggtcaactat cacgaatgcc tcaatcccta ctccttttct cttcattgac tggaagaaaa
      361 gcggagtata ccattgacgg aaaggcagat aactctcgat tcgcattgga gggaaaaagg
      421 tgttcactat caaggaattc cttcggactg aagtcaaacc cgcgctcgga tgtaccgagc
      481 ccggagcggt ggcgctggcc gtcgcccggg caaaggaaga actcggcgga cctgtggatg
      541 ccgtttcggt gaccgtcagc gacagcatct acaagaacgg cgtcgatgtc ggcattcccg
      601 ggacggaggg gctgagggga aacagcgtgg cggcggccct ttcggccttc tgcggccgct
      661 ccgcctacgg cctcgaggtg ctcaaggact gtacggaaga ccatatcgca tcggcaaagg
      721 ccatgctcgg gaaaaacgca gtccaaatca accccgacct cgaccgccat ggagtctacg
      781 tagaagcgtc cgtccgttcc ggagaagaca gggcggtctg ccgcatcgaa ggctcccaca
      841 caggcatttc ggaagtaacc ctcaacggaa agaccacctt ctcagccgaa aaaaccccgt
      901 cctcggcgcc gaacggcccc gaagcaaatg tttcagatga aatagcccga atgtcctttc
      961 cagaccttgt ctccctggtt gaaaccatgg acggtgagga catggattat gtcatgagcg
     1021 gcgtggaaat gaacatgacc atcgcggagt acggattcga cacctcccac ccgacggggc
     1081 tcgacatcgg caaaaccctg aaaaaactca gcggagcggc cttcaactcc gaggacctca
     1141 gcctcaggat caaggcatac gccgcggcag catcggacgc ccgcatggca ggcgctcccc
     1201 tcgccgtcat gagcagcgcc gggagcggca accacggagt gacggccatc ctccccgtcg
     1261 ccgtctgcgc gcagcaccac ggaaaaagcc gggaggaaac cgccagggcc gtagccttca
     1321 gccaccttgc aacaagctat atcaaaagca ggaccgggcg gctcaccccc acgtgcggct
     1381 gcaccgttgc cgccggcgcc gga
//
LOCUS       DAOTHD010000695         1403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_110748_length_1403_cov_2.236647, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000695 DAOTHD010000000
VERSION     DAOTHD010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110748_length_1403_cov_2.236647"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="P5219_10525"
     CDS             complement(<1..662)
                     /locus_tag="P5219_10525"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRX27242.1"
                     /translation="MATLLLASMLMLNNAELYYEVHGVDGPPLLLVAGLASDVSSWQT
                     VLPVLSEHFRVIVVDNRGIGRSLPEDAPVSVDLMADDCAALIDHLGYGPVYVLGHSMG
                     GFTALNLAARYPEKVRKLVIAASGEKSSSRNTLLFSDLADRYEKSEDLAGFFRTVFYW
                     IFSPAFFEDEGTVKGAVEFALSYPFPQAPVSFRNQVNAIAAFDGRGLAASISVPALVL
                     AGT"
     gene            complement(750..>1403)
                     /locus_tag="P5219_10530"
     CDS             complement(750..>1403)
                     /locus_tag="P5219_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27243.1"
                     /translation="NSVAGSDQRTSHELSHTAPSGKPANKAAYHHYKVSILASTNKQT
                     RFSLKIIQRGNNKNLDREYLERAKKQLDVLAKAISNERKRLSGAMNQQAERIKGIDEH
                     QKSWKARLDTERAELARMADAIRSEQNKAARSSMVQTYKFRQADFNAKVEAFNRENAK
                     RRALYNDWKGVREQRDTLDSFAKSILEAWKQNDIDRCVLIANGSRLAKSLGWRFMKR"
BASE COUNT          261 a          425 c          407 g          310 t
ORIGIN      
        1 gtgccggcca gaaccagcgc gggaacgctg atggaggcgg caaggccccg gccgtcgaag
       61 gccgcgatgg cattcacctg gttccggaac gagacgggag cctgggggaa gggataggag
      121 agggcaaatt cgacggcgcc tttcaccgta ccctcgtcct cgaaaaaggc gggagaaaaa
      181 atccagtaaa agaccgtccg gaagaatccg gccaaatcct cgcttttctc ataccggtcg
      241 gccaggtcgg agaaaagcag ggtgttcctc gaagagcttt tctcgcccga tgcagcgatt
      301 acaagttttc tcaccttttc gggataccgt gcggcaaggt tcagggcggt gaatcccccc
      361 atggaatggc ccagcacgta gacagggccg tacccgagat ggtcgatcag ggcggcgcag
      421 tcgtccgcca tgagatccac ggaaaccggc gcgtcctccg ggagcgagcg gccgattccc
      481 cggttgtcca caacgatcac ccggaaatgc tccgacagga caggcaaaac cgtctgccag
      541 ctcgaaacat ccgacgccag accggcaacg agcagcaatg gaggcccgtc gacgccgtgc
      601 acctcgtaat acagttccgc gttgttcagc atcagcatgg aggccaaaag cagcgtagcc
      661 attccacaac ccctttcggc aggataggga catcttaaca ccgttgcctg cccggaaaaa
      721 gggcgaatat gccgccgctg tttttccggc taccgcttca tgaacctcca gccgagggac
      781 ttggccagcc ggctcccgtt ggcgatgagg acgcagcggt cgatgtcgtt ttgcttccag
      841 gcctctaata tggatttcgc aaagctgtcc agtgtgtccc gctgctcccg tactcccttc
      901 cagtcgttgt agagcgccct ccgttttgcg ttttcccggt tgaaagcctc cactttcgcg
      961 ttgaaatcgg cctgcctgaa cttgtacgtc tggaccatgg acgaccgtgc cgccttgttc
     1021 tgttcgctcc ggatcgcgtc cgccatcctg gcgagctcgg cccgttcggt gtcgagccgg
     1081 gctttccagc tcttctgatg ttcgtctatg ccttttatcc tctcagcctg ctgattcatt
     1141 gcgccgctca ggcgcttccg ctcgttcgat atggcctttg ccaggacatc cagctgtttt
     1201 ttggcccgct ccaagtattc cctgtcgagg tttttgttgt tcccccgctg gatgattttc
     1261 agcgaaaacc gggtctgctt gttcgtcgac gcgagaatgc tgaccttgta gtgatggtag
     1321 gcggctttgt tggccggttt cccggagggg gccgtgtggc tcagctcgtg ggacgttctc
     1381 tggtcactac cagccacgga att
//
LOCUS       DAOTHD010000696         1400 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_111054_length_1400_cov_2.855762, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000696 DAOTHD010000000
VERSION     DAOTHD010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111054_length_1400_cov_2.855762"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="P5219_10535"
     CDS             <1..230
                     /locus_tag="P5219_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27244.1"
                     /translation="PSEAVFAGKGTEHFVWKISGTDPLKVTAVPVLVSGFRADMAEVK
                     GELAPGDRIVTAGVSFLQEGDPVTLYEGPRR"
     gene            243..>1400
                     /locus_tag="P5219_10540"
     CDS             243..>1400
                     /locus_tag="P5219_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRX27245.1"
                     /translation="MNFADFSLRKSTITWFLTVLVVIGGVWAYGRLGKLEDPSFTIKT
                     AAISTTYPGASPREVEEEVTALIESAAQKLGQTDKVRSLSKEGVSIVYVDIRDTYTAK
                     DLPQIWDELRRKISDIQSSLPPGAGPSVVNDDYGDVFGVYFALTGEGYTFRELEDHGD
                     FLRRELLLVPGVANVEIAGIRQEVIYLEMDRTRMAALGISLDEISNLLSAQNVVTGAG
                     SAAVGPELLRIAPTGYFSSLDVLENLLLRAGSGQTLRLGDIASVSRGYMEPPARAMRF
                     NGKPALGIGVSNVDGGNVITIGEAVKQRLRELLPRTPVGMEMHLIYYQADTVKESIDN
                     FVTNLLEALAIVIGILLVFMGLRSGLLIGAMLLLTILATFVAMKLAAIDLHS"
BASE COUNT          279 a          442 c          418 g          261 t
ORIGIN      
        1 tgccttccga agcggtcttc gccggaaagg gaacggagca tttcgtgtgg aaaatatcgg
       61 gcacggatcc cctgaaagtc acggcggttc cggtgctggt ttccggcttc cgggccgaca
      121 tggcggaagt gaagggagag ctggcgccgg gagacaggat cgtcacggct ggagtgtcct
      181 ttctgcagga gggcgacccg gtcaccctct acgaaggacc acgccggtag cggaggggca
      241 ccatgaattt cgccgatttt tccctgcgga aaagcaccat cacatggttt ctcaccgtcc
      301 tggtcgtcat aggaggcgtc tgggcctacg ggaggctcgg aaaacttgaa gacccgtctt
      361 tcaccatcaa aacggcggcc ataagcacga cctaccccgg tgcctcaccg cgggaggtag
      421 aggaagaggt aacggctctc atcgaatccg ccgcacagaa actcggccag accgacaagg
      481 tccggtccct ctcgaaagaa ggagtgtcca tagtttacgt ggacatcagg gatacctaca
      541 cggcgaagga tcttccccag atatgggacg aacttcgccg gaaaatatcg gatatccagt
      601 ccagcctccc cccgggagcg ggcccgtcgg tggtgaacga tgactacggc gatgtcttcg
      661 gcgtgtattt cgccctcacc ggggagggct ataccttccg ggaactggag gaccacggtg
      721 actttctgcg ccgtgagctc cttctcgttc cgggcgtggc caacgtggaa atcgccggca
      781 tccggcagga ggtcatctac ctggagatgg accgcacccg catggctgcc ctggggatct
      841 ccctggacga aatctcgaac cttctttccg cccagaacgt ggtcacagga gcgggttcgg
      901 cagccgtggg gccggagctg cttcgcatag cccccacggg atacttttcg tccctggacg
      961 tcctggaaaa cctcctgctc cgggcaggct ccgggcagac cctccggctc ggcgacatcg
     1021 cctcggtatc cagaggctac atggaacctc cggcaagggc catgcggttc aacggcaaac
     1081 ccgccctcgg cataggcgtc tccaatgtgg acgggggcaa cgtgatcacc atcggcgaag
     1141 cggtgaagca gaggctccgg gaactcctcc cccggacccc cgtggggatg gagatgcatc
     1201 tgatctacta ccaggcggac acagtcaaag agtccatcga caacttcgtc acgaaccttc
     1261 tcgaggccct cgccatagtc atcgggatcc ttctcgtctt catgggcctt cggagcggcc
     1321 tgctcatcgg cgccatgctc cttctcacca tcctcgccac cttcgtcgcc atgaaactcg
     1381 cggccatcga cctccacagt
//
LOCUS       DAOTHD010000697         1400 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_111069_length_1400_cov_2.483271, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000697 DAOTHD010000000
VERSION     DAOTHD010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111069_length_1400_cov_2.483271"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="P5219_10545"
     CDS             <1..250
                     /locus_tag="P5219_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HRX27246.1"
                     /translation="GGPNPNAGKLWLNYILSSEGQGLFAKGFVRPILPGFEMPADVAD
                     KFLPASDYGRAKDVDWVKAQKVQKEAASLWGSKVLGEN"
     gene            256..>718
                     /locus_tag="P5219_10550"
     CDS             256..>718
                     /locus_tag="P5219_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27247.1"
                     /translation="MERRSGTWLLLLPLAAVLGLFLLWPLALVLWESLFIEGRFSLDN
                     YLSLFVRKLYRESLTTSLVLSASTAVLGTAVGLPLSYFVHTTRGRAKNILLALTAVPL
                     TLSGLVVGFAFIVLLGTSGFITIILRELFGINPLEFSAFLFTWRGLVIAYLY"
     assembly_gap    719..728
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <729..1103
                     /locus_tag="P5219_10555"
     CDS             <729..1103
                     /locus_tag="P5219_10555"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRX27248.1"
                     /translation="YFLIPRMILTMTAAWSNANWSLVEAAVSLGAGPVTVLRKVLFPM
                     LAPAILAGSSLLFAVSMGAFGTAFALTGTAVKILPLVIYTHVSELSADIAKADALAIV
                     LTLVTTGVILAYEKFFTSPSSR"
     gene            complement(1107..>1400)
                     /locus_tag="P5219_10560"
     CDS             complement(1107..>1400)
                     /locus_tag="P5219_10560"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HRX27249.1"
                     /translation="NSAGPIMSLYFLIAGFPKLPFIATAAWFFFTMNLLKVPLFLNLG
                     LISGQSLRVNLLTIPSVALGALLGYWIIGRISQEKFNRIVLLLAFVAALRLVF"
BASE COUNT          254 a          446 c          411 g          279 t
ORIGIN      
        1 gggcggcccc aaccccaacg cgggaaagct ctggctgaac tacatcctct catcggaagg
       61 gcaggggctc tttgcgaaag ggttcgtccg gcccattctc ccggggtttg agatgccggc
      121 ggacgtggcg gacaagttcc tcccggcgtc ggactacggc cgggcaaagg acgtggactg
      181 ggtgaaggcc cagaaggtgc agaaagaggc agcctctctc tggggcagca aagtgctggg
      241 ggagaactag agaccatgga gcgccggagc ggaacctggc ttttgctgct gcctctcgcg
      301 gcggtgctgg ggctgtttct gctctggcct ctcgcgctcg ttctgtggga gagcctcttc
      361 atcgagggga ggttctccct tgacaactac cttagcctgt tcgtcaggaa actctaccgg
      421 gaatccctga ccacgagcct cgtcctctcg gcgagcacgg ccgtcctcgg aactgccgtc
      481 ggcctgcccc tctcctactt tgtccacacg acccgcggcc gggcgaaaaa tatcctcctt
      541 gccctcacgg ctgttcccct gaccctgagc ggtctcgtag tgggcttcgc tttcatcgtg
      601 ctcctgggga ccagcggctt cattaccatt attctcaggg aactcttcgg catcaacccc
      661 ctcgagttct ccgccttcct tttcacatgg agagggctcg tcatcgccta cctttactnn
      721 nnnnnnnnta tttcctcatc cccaggatga tcctcaccat gacggcggca tggagcaacg
      781 ccaactggag cctcgtggaa gccgccgtat ccctcggcgc cggacccgtg acggtcctcc
      841 gcaaagtcct cttccccatg cttgccccgg ccatcctcgc cggctcgtcc ctgctcttcg
      901 ccgtgagcat gggagccttc ggcacggcct tcgccctcac cgggacggcc gtgaaaatcc
      961 ttcccctcgt catctacacc cacgtttcgg aactgtcggc ggacatagcg aaagcggacg
     1021 ccctggccat cgtgctgacc ttggtgacca ccggcgtcat tctcgcctac gaaaaattct
     1081 tcacgtcccc gtcgtcccgg tagcggtcag aacaccagcc gaagggcggc gacgaaggcc
     1141 aggaggagga cgatcctgtt gaacttctcc tgggaaatcc tgcctattat ccagtaccca
     1201 agaagcgccc cgagggcgac cgacggaatg gtgagcagat tgacccggag gctttgcccg
     1261 ctgatgagcc cgaggttgag aaatagcggg accttcagca ggttcatggt gaagaaaaac
     1321 catgcggcgg tggcgatgaa gggcagcttg gggaaacccg cgatgaggaa atagaggctc
     1381 atgatggggc cggcggaatt
//
LOCUS       DAOTHD010000698         1399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_111152_length_1399_cov_3.159970, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000698 DAOTHD010000000
VERSION     DAOTHD010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111152_length_1399_cov_3.159970"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..744
                     /locus_tag="P5219_10565"
     CDS             166..744
                     /locus_tag="P5219_10565"
                     /inference="COORDINATES: protein motif:HMM:NF036878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRX27250.1"
                     /translation="MKKYIYIDYENLPGVQVENIEDAKILIFLGENQKKIPTETIRKT
                     QPMGDRVEWVTISGVGRNALDFHIAYYLAKNHTDPSSCHYIVSMDAGYDPLIKHVALF
                     GQKVKRIITLEDIKEKPTLSKDLAPKYAKVLEILKKQDKTRRPKSRKTLSSSIDTLFQ
                     GQATKEDIELIVEHLFRERFIEEKNKRIAYLD"
     gene            complement(824..>1399)
                     /gene="dgoD"
                     /locus_tag="P5219_10570"
     CDS             complement(824..>1399)
                     /gene="dgoD"
                     /locus_tag="P5219_10570"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HRX27251.1"
                     /translation="MARVLMKELEPFRLMFIEEPVLVEHREAFRELRRHTVTPIATGE
                     RNFSRWDFKDLITGGGVDILQPDLSHAGGIWEVRKIAAMAEAYDIAMAPHCPLGPIAL
                     AACLQLDFCTPNAFIQEQSLEIHYNRETDLLDYLEDPSVFRYRDGFVSLPEGPGLGIK
                     VNERKVLEKSREGHNWHNPVWRNADGSVAEW"
BASE COUNT          313 a          420 c          400 g          266 t
ORIGIN      
        1 cacggaagac ctgctgttct ccgcctccgc cggacgggcg ctggcacaaa atctttcccg
       61 gggaaagtac ctggccattg aaggagccgc ccactccatc cacgcggaaa aaccgaagga
      121 attcgccgaa gcggtgcttt cctttctgaa agagggagac aatccatgaa gaagtatatt
      181 tacatcgact acgagaatct gccgggagtg caggtggaga acatcgagga cgccaagatc
      241 ctcattttcc tcggggagaa ccagaagaag attcctaccg aaaccatccg gaagacccag
      301 cccatgggcg accgggtgga gtgggtaacc atcagcggcg tggggcggaa cgctctggat
      361 ttccacatcg cctactatct tgcgaagaac cacaccgacc cgtcctcctg ccactacatc
      421 gtctcaatgg acgccggata cgacccgctg atcaagcacg tggccctctt cggccagaag
      481 gtcaagcgca tcatcaccct ggaggacatc aaggaaaagc ctaccctcag caaggacctg
      541 gcgccgaaat acgccaaggt ccttgaaatt ctcaagaaac aggataaaac ccgccgcccc
      601 aagtccagaa agaccctttc ctcgagcatc gacaccctgt tccaggggca ggccacgaaa
      661 gaggacatcg aacttatcgt tgagcacctc ttccgggagc ggttcatcga ggagaaaaac
      721 aagcgcatcg cctatctcga ctgaagagag ctcccggcaa acccgttttt tcgcgggatg
      781 agagtgacgg aaagaagaag cccccggcaa ccgggggctt cttttaccat tctgcaaccg
      841 acccgtcggc gttccgccag accgggttgt gccagttgtg tccttccctg cttttttcca
      901 gcacctttct ctcgtttact ttaattccca gtcccggccc ttccggaagg gagacaaaac
      961 catcccggta ccggaagacg gagggatctt ccaggtaatc gagcaggtcc gtctccctgt
     1021 tgtagtgaat ttccaggctc tgctcctgga tgaaggcgtt gggggtgcag aaatcgagct
     1081 gaaggcacgc cgccagggct atggggccca gggggcagtg gggggccatg gcgatgtcgt
     1141 aggcctctgc catggcggcg attttccgga cctcccagat gcctcccgcg tggctcaggt
     1201 cgggctggag gatatcgacg ccccctccgg tgatgagatc cttgaagtcc cagcggctga
     1261 agttccgctc tccggtggcg atgggggtga cggtgtgccg ccggagttcc cggaaggcct
     1321 cccggtgttc cacgagcacg ggctcttcga tgaacatgag gcggaagggc tcgagctcct
     1381 tcatgagcac ccttgccat
//
LOCUS       DAOTHD010000699         1399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_111159_length_1399_cov_2.947173, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000699 DAOTHD010000000
VERSION     DAOTHD010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111159_length_1399_cov_2.947173"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="P5219_10575"
     CDS             <1..963
                     /locus_tag="P5219_10575"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HRX27252.1"
                     /translation="IARSVAGISAILGGHSHTLMEREEFVEGPGGWVTVIGQAGSYAR
                     YLGRMDLVLSEGMADREGTSWRAIPLEKDIPEDPRVALLIEPFRERLKEKLDIPLAPQ
                     PEDFDARRETLRTGEAPLGNFLADAFRWKAGADVAFIAGGSIRGDRIYPAGSATYATL
                     TNMMPFGGSLWKGSLSGSDLLLVLETSASGYALGEENYDAAMRVPTGGFLQVSGLRFS
                     IDPGRQPLLIDNNGIVRTRGSRLVKAEVRQPDGSWAPVDPKRIYTVATTDWTAGGGDK
                     HYVLKKNGSAFSSMEQMYLEAAADYIRFLKEMGGKAEGRITILR"
     gene            978..>1399
                     /locus_tag="P5219_10580"
     CDS             978..>1399
                     /locus_tag="P5219_10580"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRX27253.1"
                     /translation="MERTTVGYEKLRNYVADMLEKGLGYSRETAETGAWVLVEADARG
                     HASHGTARVELYHNLIAKGKADPHGKPEIVHETPVSLVLSGNNGSGFSAAKMAMERTI
                     EKARQSGTCMTTVRDSGHFGMAGVWAEMAADAGMMGVAL"
BASE COUNT          288 a          419 c          466 g          226 t
ORIGIN      
        1 atcgccaggt ccgtggcggg tatttccgcc atcctcgggg ggcacagcca caccctcatg
       61 gagcgggaag aattcgtgga gggccccggc ggctgggtca ccgtcatagg ccaggcagga
      121 tcctatgcca gataccttgg gaggatggac cttgtccttt cggaaggcat ggcggacagg
      181 gagggaacat catggcgggc gattcctctg gagaaggaca tccccgagga tccccgggtc
      241 gccctgctga tcgagccctt ccgggagcgg ctgaaggaga agctggacat ccccctcgcc
      301 ccccagcccg aagacttcga cgcccggagg gagaccctgc ggacagggga agcccccctg
      361 gggaatttcc ttgccgacgc cttccggtgg aaggccggag ccgacgtggc ctttatcgcc
      421 ggaggcagca tccgggggga ccggatctac ccggcaggat cggccaccta cgccaccctc
      481 acgaacatga tgcccttcgg cggttccctg tggaaggggt ccctctccgg cagcgacctg
      541 ctcctggtgc ttgaaacgtc cgcttcgggc tacgccctgg gagaagagaa ttacgacgcg
      601 gccatgaggg ttcccaccgg gggcttcctc caggtctcgg ggctgaggtt ctccatcgac
      661 ccgggaaggc agcccctgct catcgacaac aacggaatcg tccgcacccg ggggtccaga
      721 ctcgtgaagg cagaggtgcg gcagcccgac ggctcgtggg ctcccgtgga cccgaaacga
      781 atctacaccg tggccaccac cgactggacg gcggggggcg gtgacaagca ctacgtcctg
      841 aagaaaaacg gttccgcctt ctcctccatg gaacagatgt acctcgaggc ggcggcggac
      901 tacatccgtt ttctgaaaga aatgggcggg aaagcggaag gcagaatcac cattcttcga
      961 tgacgggggt gaatttaatg gaaagaacga cggtcggata cgaaaaactc agaaactacg
     1021 tggcggatat gctggagaag ggtctcggct attccaggga gacggcggag acaggagcct
     1081 gggtcctcgt ggaggccgac gcccgtggac atgcgtccca cggtacggcc cgggtcgaac
     1141 tgtatcataa tcttatcgcc aaaggaaagg ccgaccctca cgggaagccg gaaatcgtgc
     1201 atgaaacacc cgtttcgctg gtcctgagcg gcaacaacgg ctccgggttc tccgcggcga
     1261 aaatggccat ggagaggacc atcgaaaagg cccggcagag cggcacctgc atgaccaccg
     1321 tcagggattc cggccacttc ggcatggcag gagtctgggc tgagatggcc gccgatgcgg
     1381 gtatgatggg ggttgccct
//
LOCUS       DAOTHD010000700         1398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_111270_length_1398_cov_3.198809, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000700 DAOTHD010000000
VERSION     DAOTHD010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111270_length_1398_cov_3.198809"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..464)
                     /gene="mce"
                     /locus_tag="P5219_10585"
     CDS             complement(63..464)
                     /gene="mce"
                     /locus_tag="P5219_10585"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HRX27254.1"
                     /translation="MKPTVVDHIGIAVKSIDDALAFWQSALGVKCTGVEEVEEQKVKT
                     AFLPIGDTEVELLEATSDESPVAKFIEKKGEGIHHLAIRVENLEAALAELKEKGIRLI
                     DETPRYGAGGAKIAFVHPKSTGGILLEISER"
     gene            691..1074
                     /locus_tag="P5219_10590"
     CDS             691..1074
                     /locus_tag="P5219_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27255.1"
                     /translation="MNLVTCKLCGRLFSATRGKICVTCLDEIDDLYPKVREFLRDHSK
                     ESFNVEQIADGMDLDIRYVQALVELGYLDRGVKANPEEEEEKRRKEALARQLQASLEN
                     SASAKASENAGKMYGQQRYGTGKKK"
     gene            complement(1125..>1398)
                     /locus_tag="P5219_10595"
     CDS             complement(1125..>1398)
                     /locus_tag="P5219_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRX27256.1"
                     /translation="IARKKLDYYFRKTKEDLPELENVEYKMVVKEGNPSDIIMSYARE
                     NDFNLIVIGYRGQSTIERLVVGSTASNVARYAHCSVLIYRPGRQII"
BASE COUNT          331 a          366 c          359 g          342 t
ORIGIN      
        1 tcgtgcttct tcagaagctc ctcgcacaac tcctcaattg tccggtgtga catgtaccgc
       61 acctaccttt cactgatttc caacagaatg cctccggtgg acttggggtg cacaaacgcg
      121 atcttcgcac ctccggctcc gtaccggggc gtttcgtcaa taagcctgat gcccttttcc
      181 ttcagttcag caagagcggc ctccaggttt tccaccctga tggcgagatg gtggattccc
      241 tctccttttt tctcgatgaa cttggccacg gggctttcgt ctgaagtggc ctcaagaagt
      301 tccacttccg tatcgccaat ggggagaaag gcagtcttga ccttctgttc ctccacttcc
      361 tcaacgccgg tgcacttcac gccgagggcc gactgccaga aggcgagagc gtcgtcaatg
      421 gatttgacgg caattccaat atgatctact actgtaggct tcatttaaaa ttcccctttc
      481 tattcgataa ttttcacttg atgaaacgat gaaagaaagt cagacgggca atatcttatc
      541 agaaaagcgt aaggaaggac tttcaaaaag catagaatac tgtatacacc aatcgtaccc
      601 tcctgtatct ccttcctgtt ctttcgtgcc gtctttatca gcctggggaa aaatgatata
      661 gttatccgga tagggaggga ggatgacgca gtgaacctgg taacctgtaa gctgtgcggg
      721 cggcttttca gcgcgacccg cgggaaaata tgcgtaacgt gcctcgatga aatcgacgac
      781 ctttacccca aggtccggga gttcctcagg gatcattcaa aagaaagttt taacgtggaa
      841 caaatagcag atggcatgga tctggacatc cgctatgttc aggcccttgt cgaattgggc
      901 tacctggaca ggggcgtcaa ggcgaatccc gaagaggaag aggaaaagag acggaaagaa
      961 gccctggcgc ggcagctcca ggcctccctg gaaaattcgg cctccgcgaa agcgtcggaa
     1021 aatgcgggga agatgtacgg tcagcagcgc tacggcacag gaaagaaaaa atgaaatcag
     1081 ccggggggga gaagggtgca ccccttcccc cccccacttc tttttcagat tatctgccgt
     1141 cccggtctgt agataagcac ggagcagtgg gcgtaccggg caacgttgga ggcggtgctt
     1201 ccgacgacga gacgttcgat ggtgctttgt cccctgtagc ctatgacgat gaggttaaaa
     1261 tcgttctccc tggcgtagct cataatgata tccgagggat tgccttcctt gaccaccatt
     1321 ttgtactcca cgttttcaag ctcaggaaga tcctctttcg tcttcctgaa gtagtagtcc
     1381 agcttcttcc tggcgatt
//
LOCUS       DAOTHD010000701         1396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_111517_length_1396_cov_2.963460, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000701 DAOTHD010000000
VERSION     DAOTHD010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111517_length_1396_cov_2.963460"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="P5219_10600"
     CDS             complement(<1..1077)
                     /locus_tag="P5219_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRX27257.1"
                     /translation="MILKIIGAAGEVTGSSYLIECGASRVLVDCGIFQGKDDDRKNSE
                     PFPFAAGSLDAVLLTHAHMDHSGRIPLLVKEGFKGKVFATLPTLELVKVLWYDSANLM
                     KEEAEWKTKKNSRKGLDPVEPLYSAEHVDQAIRHLSASSYDDRISVAPGVSVRFRDAG
                     HILGSAILEVWLTEDEKEVKVVFSGDLGPQETVMERNPAIITDADYVIMESTYGDRLH
                     RNNAETRDEFRDVFTRVLKNKGKVFIPSFVVDRAQRVLYELSLLKDEGILGETVPIFF
                     DSPMGVKATEIYRKHMNLLSSEIQQYVSEGKDPFSPGKLKYVSSVEDSRAINEVRHGV
                     VVAGSGMVNGGRIVHHLKHGIWDPK"
     gene            complement(1123..>1396)
                     /locus_tag="P5219_10605"
     CDS             complement(1123..>1396)
                     /locus_tag="P5219_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRX27258.1"
                     /translation="SNAVRHGQATEMRAEVFRKDGSVVFEFQDNGKGFEPDMNYETLR
                     VEGHRGLANMLERMMLVGGRLEVESRPGNGALVRCVMPDVQEAAAV"
BASE COUNT          266 a          404 c          371 g          355 t
ORIGIN      
        1 tttgggatcc catatgccat gtttcaggtg gtggacaatc cttcccccgt tgaccattcc
       61 gctgccggca acgaccaccc catgccggac ttcgttgatg gcccgtgaat cttcgacgct
      121 ggaaacatat ttcagtttcc cgggggaaaa ggggtctttc ccttcgctca cgtactgctg
      181 gatttccgag gaaagaaggt tcatgtgctt tctgtagatt tccgtcgctt tgactcccat
      241 gggagaatcg aagaatatgg gaaccgtttc cccgagaatc ccctcatcct tgagaaggga
      301 gagctcgtag aggaccctct gggccctgtc cacgacgaaa ctggggataa agaccttccc
      361 tttgtttttc agtaccctgg tgaagacgtc ccggaattca tcccgggtct ccgcattgtt
      421 cctgtggagc ctgtcgccgt aggtggattc catgatcacg tagtcggcgt ccgtaatgat
      481 cgccgggttt cgttccatga ccgtttcctg ggggccgagg tcgccgctga agacgacttt
      541 tacttctttt tcgtcttcgg tgagccagac ttcgaggatt gcactcccga ggatgtgtcc
      601 agcatcccga aaccggacgg agactccggg ggccacggat atcctgtcgt catagctgga
      661 ggccgaaagg tggcggatcg cctggtccac gtgctcggca ctatagagag gctccacggg
      721 gtcaagccct tttctggaat tcttcttcgt cttccattca gcctcttctt tcatgaggtt
      781 ggcggaatca taccagagca ctttcacaag ttcgagggtg gggagggtgg caaagacctt
      841 tcccttgaac ccctccttca caaggagagg tatcctgccg gagtggtcca tgtgggcatg
      901 ggtgaggaga accgcatcaa ggcttcctgc agcgaagggg aagggttcgc tgttttttct
      961 gtcgtcgtcc tttccctgga aaatgccgca gtctacgagc accctgctgg ctccgcattc
     1021 gatgaggtag ctggatccgg tcacttctcc ggcggcgcca atgattttaa gtatcatcga
     1081 aaattcctcc ttttgaatac aacgctccat cctattttcg ggtcacaccg ccgctgcttc
     1141 ctgaacatcc ggcatgacgc accgtacaag ggccccgttt ccgggccggg attccacttc
     1201 gagacggcct cccacgagca tcatgcgctc gagcatattg gcaagtcccc tgtgtccttc
     1261 cacccgcagg gtttcataat tcatgtcggg ttcaaatcct tttccgttgt cctgaaactc
     1321 gaagactacg gatccgtcct tccggaaaac ctccgcccgc atttccgtcg cctggccatg
     1381 ccggacggca tttgaa
//
LOCUS       DAOTHD010000702         1396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_111542_length_1396_cov_2.571961, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000702 DAOTHD010000000
VERSION     DAOTHD010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111542_length_1396_cov_2.571961"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="P5219_10610"
     CDS             <1..746
                     /locus_tag="P5219_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27259.1"
                     /translation="GFEVRFYSSPDEISSSGTAPGDLFLYIHFCGFPNRPVEEALRSL
                     PAESRPVIIEDCVHALFTRGVGTFGDFALYSFRKFLPVPDGGLISSRLPLDGRLAPPL
                     ENFISLKTAGLMAGEPAFLRQGEAVLDSDDGTREPSAMGGLLLDRLPWEHIPAARREN
                     YSFLAGRLGLPALQDGTVPLGVPFRVESGKAELERRLRGAGFEPPLSWDAPPGAPSDE
                     AEKLVILPCDERMNLKDLEELAAVCVSGL"
     gene            802..1221
                     /locus_tag="P5219_10615"
     CDS             802..1221
                     /locus_tag="P5219_10615"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRX27260.1"
                     /translation="MPEICSQITFFYYKDLAKAADFYERIMGFELADDQGTCRIYRVR
                     GSAFLGIVDERRGHCSARKSESTVLATFVVDDVREWHEHLRKNGVTITSELLSKPEIQ
                     IEAFFFEDPEGYALEAQSFLNPALRPVFGHGGAPAGR"
BASE COUNT          265 a          444 c          450 g          237 t
ORIGIN      
        1 gagggttcga ggtgagattc tactcttccc ccgacgaaat ctcctcttca ggaacagcgc
       61 cgggagatct cttcctgtac atccatttct gcggcttccc caaccggccg gtggaggaag
      121 cccttcgctc cctgcccgcg gaaagcaggc ccgtcatcat tgaagactgc gtccacgccc
      181 tttttacccg gggggtggga accttcggcg atttcgccct gtacagcttc cggaaatttc
      241 ttcccgtgcc ggacggcggc ctgatctcct cccggcttcc cctcgacgga aggcttgccc
      301 cgcccctcga aaactttatc tccctcaaaa cggcggggct gatggcgggg gagccggctt
      361 ttctccggca gggggaggcc gtgctcgaca gcgacgacgg gacacgggag ccctcagcca
      421 tgggaggcct gcttctcgac aggcttcctt gggaacacat accggccgcc aggagggaaa
      481 actattcctt cctcgccggg cggctcggcc tgccggccct tcaggacgga accgtgccgc
      541 tgggagttcc cttccgggtg gaaagcggaa aggccgaact ggagcggcgc ctccgggggg
      601 cagggttcga gccgcccctg tcgtgggacg cccctcccgg agcgccttcg gacgaggcgg
      661 aaaaactggt gatccttccc tgcgacgagc ggatgaacct caaggacctg gaagagctgg
      721 ccgccgtctg cgtgagcggg ctataatgaa gcgggggacc ggcggtctct caaccgtcag
      781 acatgcgagg aggccggtac catgccggaa atctgttctc agatcacgtt tttttactac
      841 aaagacctgg cgaaagccgc ggatttctac gagaggatca tggggttcga actggcggac
      901 gaccagggaa cctgccggat ctacagggtc cggggaagcg cattcctggg gatagtggac
      961 gagcggcgcg gccactgctc cgcgcgaaag agcgaaagca ccgtcctggc caccttcgtg
     1021 gtggacgacg tgagggaatg gcatgaacac ctccggaaga acggcgtcac gatcacctcg
     1081 gagcttctct ccaaaccgga aatacagatc gaggccttct tcttcgagga tccggaaggg
     1141 tacgccctgg aagcccagtc attcctgaac cccgccctca ggccggtctt cggccatggg
     1201 ggggctccag ccgggcggta attcccgaag ccgcttccgg cgaaggtcct atcttcttcc
     1261 gccggaagga agcagcagcg acaccaggta gaccgccgac gccaccagga tgatcaccgc
     1321 ccccgcggtg aggttgaacc gcacggccag ggcgagcccc ccgagggaga aggcggcgct
     1381 gaggaggcag gagacc
//
LOCUS       DAOTHD010000703         1392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_112031_length_1392_cov_2.383695, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000703 DAOTHD010000000
VERSION     DAOTHD010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112031_length_1392_cov_2.383695"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..560
                     /locus_tag="P5219_10620"
     CDS             120..560
                     /locus_tag="P5219_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27261.1"
                     /translation="MSIRRNAGIFWGAVLLAVLVVFCGTVSAAEKPYTPQPGNPVRKA
                     VLDGLRNWVRENHEVRVVFVVRYLKVAEGWAWAETAPQSPDGAEKYEGLMALLRRTGE
                     GWKVMHVPSGEEDAPPVDGTYFEMLLEEAPGIPRGIFPWERKVN"
     gene            complement(553..>1392)
                     /locus_tag="P5219_10625"
     CDS             complement(553..>1392)
                     /locus_tag="P5219_10625"
                     /inference="COORDINATES: protein motif:HMM:NF015056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HRX27262.1"
                     /translation="VVFNTVMLMAGNLSPDMTFDDMLALRTEMKEQGTGVYAFTGPFR
                     INGAQPGDTLEVRIRRLVPGKYAVTHIYPDHMNIGGLPEGFSRGFLKPLCLSEDRKTI
                     EFAPGVTLRTRPFLGTMAVAPRPGEVLPPAVPGYFAGNMDNKELAEGSTLYIPVNVPG
                     ALFMAADAHAVQGDGEVSIAAAETYFEEAELQFVVRKDMKLHYPLAETPTHWIVMGFH
                     PDLDEAMKMAIREAVSFLMREKGLSREEAYSLCSLAADFRVTQVVDGDKGIHGMIPKS
                     IFS"
BASE COUNT          248 a          392 c          475 g          277 t
ORIGIN      
        1 cccctccttc cccggagggg cttttttttg gccgcttctt ctataatgga gcggggagtg
       61 ttcactgctt ttctgctccg gccggtacgg aatacgggca gggaaaggag acggtagcga
      121 tgtccatcag gaggaacgcc ggtatttttt ggggagctgt acttctcgct gttctcgtcg
      181 ttttttgcgg cacagtttcc gcagcggaaa aaccctacac gccccagccg ggaaacccgg
      241 tcaggaaagc ggttctggac gggcttcgga actgggtccg ggagaaccac gaggtcaggg
      301 tcgtcttcgt ggtccgttat ctgaaggtgg ccgaaggatg ggcctgggcg gaaacagcgc
      361 cccagtcgcc ggacggcgcc gagaagtacg aagggctcat ggccctcctc aggcggaccg
      421 gggaagggtg gaaagtgatg cacgtcccgt ccggggagga ggatgctcct cccgtggacg
      481 ggacgtattt tgaaatgctc ctcgaggaag cccccgggat tccgaggggc atattcccct
      541 gggagcggaa ggtcaactga agatcgactt ggggatcatg ccgtggatgc ccttgtcgcc
      601 gtccaccacc tgggtgacgc ggaagtcggc ggcgaggctg cacagggagt aggcctcctc
      661 cctggagagt cccttttccc gcataaggaa ggagaccgct tctcggatgg ccatcttcat
      721 ggcttcgtcc aggtccggat ggaaccccat gacgatccag tgggtcggcg tttcggcgag
      781 ggggtaatgg agcttcatgt ccttccggac cacgaactgc agttccgctt cctcgaagta
      841 ggtttccgcg gcggcgatgc ttacctcccc gtctccctgg acggcatggg cgtcggcagc
      901 catgaagagg gcccccggga cgttcacggg aatgtacagc gtggatcctt ccgcgagttc
      961 cttgttgtcc atgtttccgg cgaagtagcc cgggacggcg ggaggcagca cctcgccggg
     1021 acggggggcg acggccatgg tgcccaggaa gggcctggtc cgcagggtca ccccgggggc
     1081 gaattcaatg gtcttcctgt cctccgagag gcagagaggt ttcaggaagc ccctgctgaa
     1141 gccctcgggc agaccgccga tgttcatgtg gtccgggtag atgtgggtta cggcgtattt
     1201 cccgggaaca aggcgcctga tccggacctc cagggtgtcg ccgggctggg ctccgttgat
     1261 gcggaagggg ccggtaaagg cataaacgcc ggtcccctgt tccttcatct ccgtgcggag
     1321 ggcgagcata tcgtcgaagg tcatgtccgg agaaagattc cccgccatga gcatgaccgt
     1381 gttgaaaaca ac
//
LOCUS       DAOTHD010000704         1388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_112491_length_1388_cov_2.465866, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000704 DAOTHD010000000
VERSION     DAOTHD010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112491_length_1388_cov_2.465866"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..493)
                     /gene="rpsK"
                     /locus_tag="P5219_10630"
     CDS             complement(101..493)
                     /gene="rpsK"
                     /locus_tag="P5219_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRX27263.1"
                     /translation="MAKRTVRKGKRKERKNISYGVAHIYSTFNNTIVCVSDKGGNILA
                     WASGGNVGFKGTRKSTPFAAQIAATQVAKAVQEHGVQEIDVVVKGPGPGRESAIRSLQ
                     AAGLQVNMIRDTTPIPHNGCRPPKRRRV"
     gene            complement(531..908)
                     /gene="rpsM"
                     /locus_tag="P5219_10635"
     CDS             complement(531..908)
                     /gene="rpsM"
                     /locus_tag="P5219_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRX27264.1"
                     /translation="MARIAGVDIPRDKRVEIALTYIFGIGLPTSRKILQVTGVNPDTR
                     VKDLTEEETQKLRREIEDHHKVEGDLRREVSMNIKRLMDIGCYRGQRHKLGLPVRGQK
                     TKTNARTRKGPRRTVAGKKMATK"
     gene            complement(917..1042)
                     /gene="rpmJ"
                     /locus_tag="P5219_10640"
     CDS             complement(917..1042)
                     /gene="rpmJ"
                     /locus_tag="P5219_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRX27265.1"
                     /translation="MKVKPSVKPMCEFCRVIRRKGVVRIICSRNPRHKQRQGARR"
     gene            complement(1227..>1388)
                     /gene="infA"
                     /locus_tag="P5219_10645"
     CDS             complement(1227..>1388)
                     /gene="infA"
                     /locus_tag="P5219_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRX27266.1"
                     /translation="AMFRVELDNGHRILAHVSGKIRLHFIRILPGDKVLVEISPYDLA
                     RGRIIYRYK"
BASE COUNT          249 a          416 c          366 g          357 t
ORIGIN      
        1 ccttcaggaa gagcttggtt ccctcagcct tgcactgacg gcacgaaggc cctatatatc
       61 tgctcataag aaatacattc cctccttgac tgaccttttc ctacactctg cgccgcttcg
      121 gaggacggca gccgttgtga ggaatggggg tggtgtcccg tatcatgttc acctgcagcc
      181 ctgcggcctg gagcgacctg atggcggact cgcggccagg gccggggccc ttcacgacca
      241 cgtcgatttc ctggacgccg tgttcctgga ccgccttggc gacctgggtc gcggcgatct
      301 gggcggcgaa gggggtggac tttctcgttc ccttgaagcc cacgttcccg ccggaagccc
      361 atgcgaggat gttgccgccc ttgtcgctca cgcagacgat ggtgttgttg aacgtcgagt
      421 agatgtgagc gacaccgtag ctgatattct ttctttcctt gcgtttccct ttacggaccg
      481 tacgcttggc cacttaatcc agcctccttg tttttcctgg gttcagaagg ctacttcgta
      541 gccatcttct tgcccgcgac agtgcgccgg ggtcccttgc gggtgcgggc attggtcttg
      601 gtcttctgtc cgcggacggg aagaccgagc ttgtgacgct ggcccctgta gcagcctatg
      661 tccataagcc gcttgatgtt catggacact tccctgcgaa gatcgccttc caccttgtgg
      721 tgatcttcga tctccctgcg gagcttctgg gtctcctcct cggtgagatc cttcaccctg
      781 gtgtcggggt tgacccccgt gacctggaga atcttcctgg aagtgggaag tcctattccg
      841 aaaatatacg tcaacgcaat ttccacacgc ttgtctctgg gtatgtctac tccggcgatt
      901 cgggccatag ccagccttac ctccttgcac cctgtctctg cttatggcgc ggatttctgc
      961 tgcagataat ccgcacgaca cccttgcgcc ggatgacccg gcagaactcg cacatcggtt
     1021 tgaccgaagg tttcactttc atccattcca cgctcctctg gcaaagaact tgaaaacaga
     1081 tgcccgcacc cgggcttctg taaataccgc attttctcca agcgttaaat tatacagcag
     1141 tatcggtcag aagaaaacac ttttccgtcg ctgacggttc atcttttttg aaaaacgctc
     1201 ccggtacagt ctgctccgtg cttcccttat ttgtatctat agattatccg gccccgtgcc
     1261 aagtcgtagg gggatatctc caccaggacc ttgtcgcccg gcagaatgcg gatgaaatga
     1321 agccggattt ttccggaaac atgggcgaga atcctgtgcc cgttgtcgag ttccacccgg
     1381 aacatggc
//
LOCUS       DAOTHD010000705         1388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_112519_length_1388_cov_1.755439, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000705 DAOTHD010000000
VERSION     DAOTHD010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112519_length_1388_cov_1.755439"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="P5219_10650"
     CDS             complement(<1..433)
                     /locus_tag="P5219_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRX27267.1"
                     /translation="MEKVLLISPEKCIGCGSCEMACSIENEGEFRPAVSRISVHRFEG
                     GQNVPMSCLQCEEAACMAACKTGALSRNEQGVVVVEKAKCIGCRMCVMACPFGNISYS
                     RVTKTAFKCDQCGGDPKCVKWCPTKAIEFLPADTVNLQRKRA"
     regulatory      complement(565..675)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(689..>1388)
                     /locus_tag="P5219_10655"
     CDS             complement(689..>1388)
                     /locus_tag="P5219_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRX27268.1"
                     /translation="PGTTVLEAARSAGIEVPTLCAHEGLAPEGNCRLCSVEVTERGKT
                     RLAMSCMYPVQHDGIEVRTDTERVRAARCFVLQMLADRNPKAPALRELCEEYGVTPSV
                     PDGVEPELCIRCGRCVRACEANGTSALALAGRGWEREVTPPFHEASPDCVGCLSCADV
                     CPTGRITWEEKGGKRAIWGRTFDLVECERCRDRYATVEQLRLSGIDPETARLCPRCRR
                     TAQQERMKLGSREI"
BASE COUNT          212 a          478 c          425 g          273 t
ORIGIN      
        1 acgcgcgctt gcgctgcagg ttgacggtgt ccgccggcag gaactcgatg gccttcgtcg
       61 ggcaccactt gacgcacttg gggtctcccc cgcactggtc gcatttgaag gcggtcttcg
      121 tgacgcggct gtagctgatg ttgccgaacg ggcaggccat gacgcacatg cggcacccga
      181 tgcacttggc cttttccacg accacgacac cctgctcgtt ccggctcagg gctcccgtct
      241 tgcaggccgc catgcacgcg gcctcctcgc actgcaggca cgacatcggg acgttctgcc
      301 cgccttcgaa gcgatggacc gagatccgtg aaaccgccgg ccggaattcc ccttcattct
      361 cgatggagca tgccatttcg cagctcccgc acccgatgca tttttcgggt gagataagca
      421 gcaccttttc catgatgagc gattcccccc ttttcgtttt gttttcccga gcccctgaac
      481 gtgttggaca ttcctccggc gaatccctcc ctatcccctg cgggcctggt cttgtaaagc
      541 acgaaagggg atccctcccc ggaatacggg aaggaatccc cttcttcgca aatacggcag
      601 gcagaaggat cgctccttcc acccgatgcc cgggcggtct cctggagatc agccgggtcg
      661 aagggtcctg tctgtcgttt cggccgtgct agatttcccg gctgcccagc ttcatccttt
      721 cctgttgagc ggtcctgcgg catcgtgggc agagccgggc cgtttcaggg tcgatcccgg
      781 agaggcgaag ctgctccacg gtggcgtacc ggtcgcggca gcgctcgcat tccaccaggt
      841 cgaaggtccg gccccatatg gcccgcttgc cgcccttctc ctcccaggtg atccggcccg
      901 tcgggcagac gtcagcgcag gacaggcagc cgacgcagtc gggggaggcc tcgtggaagg
      961 gcggggtcac ttccctttcc caaccccggc ccgcaagcgc gagcgcgctc gtcccgttcg
     1021 cctcgcaggc ccggacgcag cgtccgcaac ggatgcagag ttcgggttcg acgccgtccg
     1081 gaaccgaggg ggtcaccccg tattcttcac agagctcccg cagggctggc gccttggggt
     1141 tccggtcagc cagcatctgc aggacgaagc accgggccgc cctgacacgt tccgtgtcgg
     1201 tcctgacctc tatcccgtca tgttggacgg gatacatgca ggacatcgcc aggcgcgttt
     1261 tcccgcgctc ggtcacctcg acggaacaga ggcggcagtt gccttcaggg gcgagtcctt
     1321 cgtgcgcgca gagagtcggg acctcgatcc cggcacttcg cgcggcctcc agtacggtcg
     1381 ttccgggc
//
LOCUS       DAOTHD010000706         1387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_112585_length_1387_cov_2.709459, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000706 DAOTHD010000000
VERSION     DAOTHD010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112585_length_1387_cov_2.709459"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="P5219_10660"
     CDS             complement(<1..442)
                     /locus_tag="P5219_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27269.1"
                     /translation="MKILRKSILAFLAVLVLAAAAFASGGRVTLLSLNDIHGHIYPED
                     GKGGLAKAATVIEAIRGENPGNTFLFEIGDVNEGPLFFYFHGHAEMRGLSLLGTDGGT
                     LGNHEFDLGEDVLFETVSRARFPIVVSNLRYRDGRPAPFPTHGIK"
     gene            complement(481..>1387)
                     /locus_tag="P5219_10665"
     CDS             complement(481..>1387)
                     /locus_tag="P5219_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRX27270.1"
                     /translation="SGEAMSVLWGALKSCLLPGDRLLAVSNGVFGRGFGEMGQALGME
                     VRIVEAPDGDFPDPEVIRREALAFRPRMITAVHCETPGGLLNPVAPLGGIAEEAGALL
                     CVDYVASAGGADVRTDGWGIDIGLLGSQKVLSLLPDLSMAAVSPKAWKAMERVNYQGY
                     DALLPWRDGVRNRYLPYTFNWQALSALRLSLLRLLGEGVEASFERHERTARYCRERLA
                     AMGVSLYPKREELCSPTVTAALVPEGWSWKMLDGALRERGMAVGGSYGPLAGKVFRIG
                     HMGSQADMELVKNGMDVLEDILGEK"
BASE COUNT          228 a          462 c          445 g          252 t
ORIGIN      
        1 tttttatgcc gtgggtgggg aagggggccg ggcggccgtc cctgtacctc aggttggaaa
       61 ccacaatggg gaaccgggct cgggacaccg tttcgaacag cacgtcctcg ccgaggtcga
      121 actcgtggtt gccgagggtg cccccgtcgg tgccgagcag ggagagcccc ctcatctcgg
      181 catggccgtg gaagtagaaa aagaggggtc cttcgttcac gtctccgatt tcaaaaagga
      241 aggtgttgcc ggggttttcc ccccggatcg cttcgatcac cgtggcggcc ttggcgaggc
      301 cgcctttgcc gtcctcgggg tagatatggc cgtggatgtc gttcagcgac agcagggtga
      361 ccctgccgcc ggaagcgaag gccgccgccg cgagaaccag gaccgccagg aacgcaagga
      421 tgcttttccg caggattttc atggccgtac ccctttcttt ccgtccggaa cgcccgattt
      481 ttacttttcg ccgaggatgt cctccaggac atccatgccg tttttcacca gctccatgtc
      541 agcctgggat cccatgtgcc ctatgcggaa cacttttccc gccagggggc cgtagctgcc
      601 tcccacggcc attccccgtt ctctgagggc gccgtccagc atcttccagg accacccttc
      661 cggcacgagg gcggcggtta ccgtggggga gcagagttcc tcccgcttcg ggtagaggga
      721 cacgcccatt gccgccagcc gctccctgca gtaccgggcg gtccgttcat ggcgctcgaa
      781 ggaggcttcc acgccctccc cgagcagcct gagcagggag agcctgaggg cgctcagggc
      841 ctgccagttg aaggtgtagg gaagataccg gttcctcacg ccgtccctcc aggggaggag
      901 ggcgtcgtat ccctggtagt tcacccgctc catggccttc caggctttcg ggctcaccgc
      961 cgccatggag aggtcgggaa gaagggaaag gactttctgg ctcccgagga ggccgatgtc
     1021 gatgccccac ccgtcggtgc gcacatcggc gcctccggca ctggccacgt agtccacgca
     1081 gagaagggcc cccgcctctt ccgcgatccc gcccagggga gcgacggggt tcagcaggcc
     1141 tccgggcgtt tcgcagtgga cggcggtgat catacggggc cggaaggcca gggcttccct
     1201 cctgatgacc tccggatcgg gaaagtcacc gtcgggagcc tccacgatcc ggacctccat
     1261 tccgagggcc tgccccattt cgccgaaccc ccgcccgaag acgccgttgg atacggccag
     1321 cagcctgtct ccaggcagga ggcagctttt cagggccccc cagagaacgg acatggcctc
     1381 gccgctc
//
LOCUS       DAOTHD010000707         1387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_112610_length_1387_cov_2.296547, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000707 DAOTHD010000000
VERSION     DAOTHD010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112610_length_1387_cov_2.296547"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="P5219_10670"
     CDS             complement(<1..204)
                     /locus_tag="P5219_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27271.1"
                     /translation="MKKSLAAALLFAVFFLLPSAARAGVGRTAPSGTVVFALREQSAG
                     EKGTLSARSLLAGAGIFSSGIQRL"
     gene            complement(224..1243)
                     /locus_tag="P5219_10675"
     CDS             complement(224..1243)
                     /locus_tag="P5219_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HRX27272.1"
                     /translation="MKTYELFKIKDLVLKNRIVMPPMCMYSAPDSIPTEFHFLHYGAR
                     ALGGAGLIIVEATGVVPEGRISDNCLGLWSDDQVAPLARLADHVHGLGAKIGIQLNHA
                     GRKCGAAVGKIYGPSALNYSDDGKYPDPVEMPGEDIGFVAGAFASAAGRALKAGFDVV
                     EIHGAHGYLVNQFLSPLSNKRHDGYGVNFEGRSRFLQDVVRAVRTEWPEEKPLFLRVS
                     AEDYAEGGMTPTEMARIIDRVTRDVDVVHVSSGGVTPVPPPVFPGYQIPFAEMIIVAC
                     EVPTIAVGLITTLEMVEEALCNHRADLVALGRELLRNPFFPLLEARKRGIELPWPEQY
                     KRAFS"
BASE COUNT          268 a          409 c          451 g          259 t
ORIGIN      
        1 gagtctttga attccggagg aaaaaatgcc cgctcccgcg agaagggatc gggcggaaag
       61 cgttcctttt tcgccggcag actgttcccg gagggcgaac accaccgtgc cggagggggc
      121 ggtacgcccc accccggcac gggcggcgga aggaagcagg aaaaataccg caaaaagcag
      181 cgcagcggca aggctttttt tcatgggtgc ccggcggcgt acctcagctg aaggcccgct
      241 tgtactgttc cggccagggc agctcgatcc cccgcttccg ggcttcaaga agggggaaaa
      301 aggggttcct gaggagttcc cggccgaggg ccacgagatc ggcccggtga ttgcacaggg
      361 cttcctcaac catttccagg gtggtgataa gccctacggc gatggtgggc acttcgcagg
      421 ccactatgat catctcggcg aaggggatct ggtatccggg aaacacgggg ggaggaacgg
      481 gcgtcacccc gccggagctg acgtggacca catccacgtc ccgggtcacc cggtcgatga
      541 tgcgggccat ctccgtgggg gtcatgcctc cttcggcata gtcctcggcg gagacccgca
      601 ggaacagagg tttctcctcc ggccattctg tgcgcacggc ccggacgacg tcctgcagga
      661 accgtgaacg gccctcgaag ttgacgccgt atccgtcatg ccgtttgttg ctcagagggg
      721 agagaaactg gttcaccagg tagccgtggg cgccgtggat ctccaccacg tcgaatcctg
      781 ccttcagggc ccgcccggca gccgaggcaa aggccccggc gacgaagccg atgtcctccc
      841 cgggcatttc gacggggtcg ggatattttc cgtcgtcgct gtagttcagt gcgctggggc
      901 cgtaaatttt cccgacggct gctccgcact tccggccggc atggttgagc tggatgccga
      961 ttttcgcccc caggccgtgc acatggtccg ccagccgggc gagaggagcg acctggtcgt
     1021 cgctccagag gccgaggcag ttgtcggaga tgcgtccctc cggaacgact cccgtggctt
     1081 ccacgatgat gagccctgcg cccccgagag cccgggcacc gtagtggagg aaatgaaatt
     1141 ccgtgggaat cgaatccgga gcgctgtaca tgcacatggg gggcatgacg atccggtttt
     1201 tgagaacaag atccttgatt ttaaaaagtt cgtaggtctt cacgaagaac aggcctcctt
     1261 tatgacagca tggtatggtg agagtagggt atcacgaaag gttccttttt gcattgaaaa
     1321 aaacgccgcc tccggtccgg aggcggcgca gcagtcgttc gcgttttctg taaccctagg
     1381 accagcc
//
LOCUS       DAOTHD010000708         1382 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_113231_length_1382_cov_2.095705, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000708 DAOTHD010000000
VERSION     DAOTHD010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113231_length_1382_cov_2.095705"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1382)
                     /locus_tag="P5219_10680"
     CDS             complement(<1..>1382)
                     /locus_tag="P5219_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27273.1"
                     /translation="AALCVTWSAPERLDEYRPLFEKALDGLREKGKTHLLATELGEIL
                     GLPVENPVARNLPLHGILPRRENWMLALSALSALGETGGGEEKRARRILWEADWECDD
                     DGIIRSVSFTPLEQVLQPSGNWSKGRPVALKRLRNDLVREDWMTRQDTAAAGAVRETS
                     GYEGWYYRRWFEFDQAKILKALAGHPAVVRADDGRPVEIVAEPPLVETETLPSGVLLR
                     MAPHPSEGPFQGLVAVEEGPERIRAVVFEEKHLEIARILGEKGIPVPPEGKGEALRAL
                     EALAPLVPVSSGLDGPGDLPGVPADERIYIQLAPGGEGFTAAFSVRPRGPGTPAGRPG
                     TGGKTVFGLSGGKRMKALRSLDEEIRRADEVRLLCPALGEGEQTDDYRWILDSPELCL
                     DFLLQAGELGDRAVIEWPRGDRLSIRGKAGASSVRASVRSVKDWFALTGEIRVDEETV
                     VSLREACRMI"
BASE COUNT          211 a          480 c          455 g          236 t
ORIGIN      
        1 agatcatgcg gcaggcctcc cggagggaga ccaccgtctc ctcgtccacc cggatctccc
       61 cggtcagggc gaaccagtcc ttcaccgacc ggaccgaagc cctgacggac gaggcgccgg
      121 cctttccccg gatcgacagc ctgtctcccc ggggccattc gatcacggcc cggtcaccga
      181 gctctccggc ctggaggagg aagtcgaggc agagctcggg ggagtcgagg atccaccggt
      241 agtcgtccgt ctgctctcct tcccccaggg cggggcagag cagacggacc tcgtcggccc
      301 tccggatctc ctcgtcgagg gaacggagcg ctttcatcct ctttcccccg gaaaggccga
      361 acaccgtttt ccctcccgtt cccggcctgc cggccggagt ccccggtccg cggggccgga
      421 cggaaaaggc ggcggtgaag ccctccccgc cgggggcgag ctggatgtag atccgctcgt
      481 ccgcaggaac tccgggaagg tcccccgggc cgtccaggcc ggaactcacg ggaacgagag
      541 gcgccagggc ctcgagggcc cggagggctt cccctttccc ttcgggggga acggggattc
      601 ctttctcgcc gagtatccgg gctatctcca ggtgcttttc ctcgaatacg acggcacgga
      661 tgcgctccgg cccttcctcg accgccacaa gaccctggaa gggaccctcg gacgggtggg
      721 gcgccatgcg gagaagcact cctgagggga gggtttcggt ctccaccagg ggaggctcgg
      781 ccacgatctc caccggccgg ccgtcgtcag cccggacgac ggccgggtgt ccggcgaggg
      841 ccttcaggat cttggcctgg tcgaactcga accatcgcct gtaataccat ccctcgtatc
      901 cgctcgtctc ccggaccgca ccggcggcgg ccgtgtcctg gcgggtcatc cagtcctccc
      961 ggacgaggtc gttccgaagg cgcttcaggg ccacgggccg tcccttgctc cagttgcccg
     1021 agggctgcag gacctgctcc aggggagtga aggagacgga ccggatgatt ccgtcgtcat
     1081 cgcactccca gtcggcttcc cagagaattc tcctggcccg cttttcctcc ccgcccccgg
     1141 tctctccgag ggccgacagt gcggagaggg caagcatcca gttttccctg cggggaagaa
     1201 ttccgtggag ggggagattc ctggcgacag ggttttccac cgggagtcca agaatttctc
     1261 cgagctccgt ggccaggagg tgcgttttgc ccttttcgcg aaggccgtcc agggcctttt
     1321 cgaaaagagg ccggtactcg tccagccttt cgggggcact ccaggtgacg cagagggccg
     1381 cg
//
LOCUS       DAOTHD010000709         1374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_114121_length_1374_cov_2.775588, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000709 DAOTHD010000000
VERSION     DAOTHD010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114121_length_1374_cov_2.775588"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="P5219_10685"
     CDS             <1..511
                     /locus_tag="P5219_10685"
                     /inference="COORDINATES: protein motif:HMM:NF014225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27274.1"
                     /translation="VDPVLGPEMRSTGEVMGTGATVGEAFAKSQAAGGTMLPVKGRVF
                     ISVNDFDKATILPVAKKLADLGFSLAATRGTAGYLFERGLFPEVVLKVHEGRPNVVDY
                     MRAGKIQMLINTPFGSNSLSGGREMRIEAVRRKIPYTTTTSAAEAAVDAIAELQKGGF
                     SVRSWAEEK"
     gene            611..1141
                     /locus_tag="P5219_10690"
     CDS             611..1141
                     /locus_tag="P5219_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1010..1012,aa:Sec)
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRX27275.1"
                     /translation="MGVSKTREGWNPYLLGGLAGLLSVWSTYYTGKFFGASTSFVRAA
                     AFVEDKVIPERAATLEYLVKNAAKMDWQMMFIIGIFIGALLSATLFGDFKFQAVPDMW
                     KRHFGESAVSRGLVAFLGGAVAMFGARLADGUPSGHGLSGTLQLAASGFLALICFFVA
                     GAIVARMIYGGGQNGS"
     gene            1131..>1374
                     /locus_tag="P5219_10695"
     CDS             1131..>1374
                     /locus_tag="P5219_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27276.1"
                     /translation="MDLDQIIVHLNEILVSLKGIANAVDLQLRFGLVTGMVFGVLLQR
                     AKVLRYDKQLAALRFQDFTILKFMMSTIIVGMIGTYF"
BASE COUNT          279 a          356 c          429 g          310 t
ORIGIN      
        1 cgtggatccg gtcctgggcc ccgaaatgcg gtccaccggg gaagtgatgg gaacgggagc
       61 cacggtgggg gaagccttcg ccaaaagcca ggcggcgggg ggaaccatgc ttccggtgaa
      121 gggaagggtg ttcatttcgg tgaacgattt cgacaaggca acaatcctcc ctgtggcgaa
      181 gaagcttgcg gatctcgggt tttcccttgc ggccaccagg ggaaccgccg ggtatctttt
      241 tgagcggggg ctgtttcccg aggtggtcct caaggtccac gaaggacggc cgaacgtggt
      301 ggattatatg cgggcgggga aaatccagat gctcatcaac actcctttcg gctccaacag
      361 cctttcggga ggcagggaga tgcgcatcga ggcagtccga aggaaaattc catatacgac
      421 gacgacttca gccgccgaag cggctgtgga tgccatcgcc gaactgcaga agggaggctt
      481 ctccgtccgt tcctgggcgg aggagaagtg accgccgcgg aaaaagaagt tgccttttcc
      541 gttattgact gtgcttcgct ttagtaatac cataaaatgg taccgcagaa tcatcatcgg
      601 caaaggaggg atgggtgtga gcaaaacgcg ggaaggatgg aatccttatc ttctcggcgg
      661 actggccgga ctgctttccg tatggtcgac ctactatacc ggaaagttct tcggcgcgtc
      721 cacatccttc gtgagggctg cggccttcgt ggaggataag gtcattccgg aacgggcggc
      781 gacacttgaa tacctggtga agaacgccgc aaaaatggac tggcagatga tgttcattat
      841 cggcattttt atcggggccc tgctttctgc cactctgttc ggcgatttca agttccaggc
      901 cgtgccggac atgtggaaga ggcatttcgg tgaaagtgca gtatccaggg gactggtggc
      961 ctttctcggc ggtgcggtgg ccatgttcgg cgcccgcctc gccgacgggt gacccagcgg
     1021 tcacgggctg agcgggacgc ttcagcttgc tgccagcgga tttttggctc tcatctgctt
     1081 tttcgtggca ggtgccatag tggccagaat gatttacggg ggtggacaga atggatcttg
     1141 atcagatcat tgtccatttg aatgaaattc tcgtgtctct caagggcatc gcgaacgccg
     1201 tcgatctcca gctccgtttc ggcctggtca ccggaatggt attcggcgtt cttctccaga
     1261 gggcaaaggt gctccggtac gacaagcagc ttgccgccct ccgcttccag gatttcacca
     1321 tcctgaagtt catgatgtcc accatcatcg tcggcatgat cggaacgtac ttcc
//
LOCUS       DAOTHD010000710         1368 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_114885_length_1368_cov_2.413557, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000710 DAOTHD010000000
VERSION     DAOTHD010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114885_length_1368_cov_2.413557"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..508
                     /gene="gpt"
                     /locus_tag="P5219_10700"
     CDS             23..508
                     /gene="gpt"
                     /locus_tag="P5219_10700"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HRX27277.1"
                     /translation="MFGVSSERYHKTYPISWEQIHRDCKALAWRLVGVAKWERIIGIA
                     RGGLVPAAIIARELNIRLVDTVCVSSYTLRTQGDISILKEIEGDGEGWLIIDDLVDTG
                     KTARVVREMLPKAHFAAVYAKPEGRAFVDTFITEVSQDTWILFPWDAETSYAKPIAEQ
                     Q"
     gene            complement(573..734)
                     /locus_tag="P5219_10705"
     CDS             complement(573..734)
                     /locus_tag="P5219_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27278.1"
                     /translation="MEGISAASRGSASQDVQLAVQYSVLKQVTDLQKDMLSQMMQSMG
                     VGQNIDLLA"
     gene            852..1316
                     /locus_tag="P5219_10710"
     CDS             852..1316
                     /locus_tag="P5219_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27279.1"
                     /translation="MHYFNYAFFLLIWGWVLSGGYVRYIVPLIGSVFSTLESMEGSGQ
                     SGPRALAFLVKIILTVAQTYVLGIWSAYCVLRTMVFLQEPGTNGWLYYISAFLICEGV
                     LGIVAKREPYRGLLSIMHSAMAMGFFVVFALNPYFLASVYPWLPPLMKFPIG"
BASE COUNT          265 a          400 c          385 g          318 t
ORIGIN      
        1 aatttttcgg cgacgaggag gaatgttcgg cgtgtccagc gaacggtacc acaagacata
       61 ccccatttcg tgggagcaga tccacaggga ctgcaaggcc cttgcctggc ggctggtggg
      121 ggtggccaaa tgggagcgga tcatcggcat cgcccgggga ggccttgtcc ccgcggccat
      181 tattgccagg gagctgaata tccggctggt ggacaccgtg tgcgtgtcca gctacactct
      241 gcggacccag ggagatattt ccatcctgaa ggagatcgag ggtgacggag aagggtggct
      301 tatcatcgac gacctggtgg acacggggaa aacggccagg gtcgtccggg aaatgcttcc
      361 gaaggctcat ttcgccgccg tctacgcgaa gcccgaggga agggccttcg tggatacctt
      421 tatcaccgag gtcagccagg acacgtggat cctcttcccg tgggacgccg aaacctccta
      481 cgcgaaaccc atagctgaac agcagtgaga aaacgaaagg ccggggggaa atgcggcatc
      541 cccccggcct tctttcatta atgtcttcct tctcaggcga gaaggtcgat gttctgtccg
      601 acgcccatgc tctgcatcat ctgggaaagc atgtccttct gaaggtcagt cacctgtttc
      661 aatacagagt actgaacggc aagctgcaca tcctgcgatg cagaaccccg ggacgcagcg
      721 gaaattcctt ccatgtgatc ccacctcccg cttttctttc gcctgattat accacagcgg
      781 agtgcccggc gaaaaaaaag agggtataat caatccgcat ccatattact ataccggaag
      841 gcggggtccc tgtgcattat ttcaactacg cgtttttcct tcttatctgg gggtgggtcc
      901 tttccggggg gtatgtccgc tacatcgttc ccctcatagg gtctgtcttc tccaccctcg
      961 aatccatgga aggaagcggc cagtccggtc ccagggctct cgcctttctg gtgaaaatca
     1021 tccttaccgt tgcccagacc tatgtactcg gcatctggtc ggcctactgc gtgctccgca
     1081 ccatggtctt tctccaggaa cccggcacga acggctggct gtattacatt tccgccttcc
     1141 tcatctgtga aggcgttctc gggatcgtgg cgaaacggga gccctacagg gggctccttt
     1201 ccataatgca ttcggccatg gccatggggt ttttcgttgt cttcgcgctg aatccctatt
     1261 ttctcgcttc ggtttacccc tggcttccgc cgctgatgaa gttccccatc ggctgaaccc
     1321 cgggcggcgg atcaggcttt cggtttgcct gccggatctc cctcgggt
//
LOCUS       DAOTHD010000711         1366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_115119_length_1366_cov_2.419527, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000711 DAOTHD010000000
VERSION     DAOTHD010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115119_length_1366_cov_2.419527"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..>1366)
                     /locus_tag="P5219_10715"
     CDS             complement(202..>1366)
                     /locus_tag="P5219_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HRX27280.1"
                     /translation="VAIPDRSSAFLEEREEFRTVHVICDVVSTERNTFDQYPRSVAER
                     TAAFLMEKNLADRAMMLVELEYYVFESVEYSTGLDHAGYSVGSSEGLGEEYSSVPRFG
                     PHKGYHKLPPEDRYLDFRNRTVHAMEQAGIPVKYHHHEVGASQLEIELDFMDLVRAAD
                     SVCVAKWIIRTVAEEMGLSVTFMPKPLYKMPGSGMHVHQFLEKGGKSLFPGDGLHGLS
                     REALAYTAGLLEHSLTGSLLAFTNPGTNSYRRLVPGYEAPISATFAKGSRSAAVRIPG
                     YLKSDEVRIEYRTGDASANIHYMLSAMVLAGADGILRNADPVAAGFDSPEAKEDKVFP
                     LNLFSVLEGLRKDNGYLTPAFPGKLLELWTKRKTEEAEYVYNAPTPQEYELYF"
BASE COUNT          225 a          389 c          472 g          280 t
ORIGIN      
        1 ccgtgggtgc gaacgtttcg gggcagatcg gctccgtgct cgctggaggg ctgttgctgg
       61 cgttcctggg gtagaactga ttcccggcag aggggagcgg caacactccc tctgcattga
      121 agaaacagat caggagaaat agaaaggggc cgtttccggc ggttgtgccg gagccggccc
      181 ctctcttttc tgtccggggg atcagaagta cagctcgtac tcctgcgggg tgggggcgtt
      241 gtagacgtat tccgcctcct cggtcttcct tttcgtccac agctcgagca gcttcccggg
      301 gaaggcgggg gtgaggtagc cgttgtcctt ccgcagcccc tcgaggacag agaagaggtt
      361 cagagggaag actttgtcct ccttcgcctc ggggctgtca aatcctgcag ccacggggtc
      421 ggcgtttctg aggatcccgt cggccccggc caggaccatg gcggagagca tgtagtggat
      481 gttcgccgag gcatcgccgg ttcggtattc tatccggacc tcatcgctct tcaggtaccc
      541 cggtatgcgg accgcggcgc ttctggagcc cttggcgaag gtggcgctga tgggggcttc
      601 gtaccccggg acgaggcggc ggtagctgtt ggtgcccggg ttggtgaaag cgaggaggct
      661 tcccgtgagg ctgtgttcga ggagccccgc cgtataggcc agggcttccc gggagagccc
      721 gtggaggccg tcgcctggga agagggattt ccctcccttt tcgaggaact ggtggacgtg
      781 cattccgctg ccgggcattt tatagagggg tttgggcatg aaggtgacgg agagtcccat
      841 ctcctccgcc acggtccgga tgatccactt ggccacgcag acgctgtcag cggccctgac
      901 gagatccatg aagtcgagct cgatctcgag ctgggaggcc cctacctcgt ggtgatggta
      961 tttcacgggg attcccgcct gctccattgc gtggacggtg cggttgcgga agtcgaggta
     1021 ccggtcttcc ggcgggagtt tgtggtatcc cttgtggggg ccgaagcggg gaacggaaga
     1081 gtactcctct ccaaggccct cggaggatcc gactgaatag ccggcgtggt ccagcccggt
     1141 ggaatactcc accgactcga agacgtagta ttcaagctcc acgagcatca tggcccggtc
     1201 ggccaggttt ttctccatga ggaaggcggc ggtccgctcg gccaccgacc gggggtactg
     1261 gtcgaaagtg ttccgctccg tggagaccac gtcgcagatc acgtgcaccg tgcggaattc
     1321 ctcccgctcc tcgaggaaag cggacgacct gtcaggaatg gccacc
//
LOCUS       DAOTHD010000712         1364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_115332_length_1364_cov_3.239878, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000712 DAOTHD010000000
VERSION     DAOTHD010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115332_length_1364_cov_3.239878"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="P5219_10720"
     CDS             complement(<1..750)
                     /locus_tag="P5219_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27281.1"
                     /translation="MHLTVSVLAALILFSMPLQGASATEEMVTVEEEFILEENSPEDD
                     RPDFYTGQDSDIFVSAEDLFGAEQEQEEFQNFDMDYPPHPEEEKALAAAMETPDEPNV
                     LSLAILRARKASSLLDSLKSGERKTPSQEGLDNPLYPALANALSAAELVPDEPDFLFL
                     VAQIYAAFGPNPFTRELAEEAAEKTLELDPSYAEARLLMGILQHQNRSFSPAIDNLER
                     AVSEKPALMDFRTASLLGAAYFLENSLPRGEQ"
     gene            complement(899..1261)
                     /locus_tag="P5219_10725"
     CDS             complement(899..1261)
                     /locus_tag="P5219_10725"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRX27282.1"
                     /translation="MAHEAGHIQLGHYNSSVGRSLLWGLLFHALDGNTAGEIAGGVGM
                     ALAESGFSREQEVAADDYGIRTSAAAGYSPWGLVRAMEKMKAAGYKTSPNGFNSHPPT
                     ERRLLRLRNNASAIEQRK"
BASE COUNT          277 a          368 c          405 g          314 t
ORIGIN      
        1 ctgctcaccc ctgggaagag agttttcgag aaaataggcg gcaccgagaa gggatgccgt
       61 acggaaatcc atcagagccg gcttttccga aaccgccctt tctaggttgt cgatggcagg
      121 gctgaatgat ctgttctggt gctgcaggat tcccatcaaa agccttgctt cggcatatga
      181 ggggtcgagt tcaagcgttt tttcagccgc ctcctctgct agctcccggg tgaaggggtt
      241 tggaccaaag gcggcgtaga tctgggcgac aaggaaaagg aagtcaggtt catccgggac
      301 aagttctgcc gctgaaaggg cgttggccag ggcaggataa agagggttat ccaggccttc
      361 ttgtgaagga gtctttcttt cgccggactt cagagaatcc aatagggatg atgctttgcg
      421 cgcccgaagt atggcgagag aaagcacgtt cggctcatca ggggtttcca tggccgccgc
      481 aagggctttc tcttcctcgg gatggggagg ataatccatg tcgaaatttt gaaattcttc
      541 ctgctcctgt tctgccccga acaggtcctc tgcggaaacg aagatgtcgc tgtcctgtcc
      601 tgtgtaaaaa tcaggcctgt cgtcctctgg gctattctcc tcgaggatga attcctcttc
      661 cacggtcacc atttcctctg ttgcggacgc cccctggagc ggcattgaaa agaggatcag
      721 agccgcaagg accgacacag tcaaatgcat tgcgaccacc tcgctttcac ccggaaaggg
      781 tacgataaga cgtctgaaaa aatcgagctg aagattggcc atattataca gcggaagcgg
      841 gatcctgcag cacaggctcc cgcttctgca aaagtggcga tggtttctgg ttattccttc
      901 atttccgctg ttctatagcc gaagcgttgt ttctcaggcg gagtagtctt ctttcagtgg
      961 gagggtggga attgaatccg ttcggcgatg tcttgtaccc ggcggccttc attttttcca
     1021 tggcccggac gagaccccag ggactgtagc cggcggcggc ggaggtcctg atcccgtagt
     1081 cgtctgcggc cacttcctgt tccctgctga agccggattc cgccagggcc atgccgacgc
     1141 ccccggcgat ttcccctgcg gtattgccgt caagggcatg gaaaagaagt ccccaaagca
     1201 ggctgcgccc cacggagctg ttgtagtgtc ccagctggat atgccccgct tcgtgggcca
     1261 tatccacgac gaggcctccc ggatggtgac gctggtggag gatatcatcc gcctgtccca
     1321 gctggacgag ggggatgcca tgcccacgga gacggtggac ctgc
//
LOCUS       DAOTHD010000713         1363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_115507_length_1363_cov_2.296636, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000713 DAOTHD010000000
VERSION     DAOTHD010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115507_length_1363_cov_2.296636"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..555
                     /locus_tag="P5219_10730"
     CDS             157..555
                     /locus_tag="P5219_10730"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HRX27283.1"
                     /translation="MAGKGKKERISVEADASPLGIPLGALLGRETPPEDPPAGKVRPE
                     AVPSTAEGKLPGRAVLSRETKGRGGKTVTRISFRDGTPPDTAALAKTLRTAMGCGGTV
                     EGDDILLQGDQIERTAEWFAARRVKVTRAH"
     gene            complement(577..939)
                     /locus_tag="P5219_10735"
     CDS             complement(577..939)
                     /locus_tag="P5219_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27284.1"
                     /translation="MAQYNVRINSEEKDGVSKEHENASGTSSSGTTQTQNTSGTPASS
                     GAGEASTENNGTCSNCGATLASGASVCGKCGTVVESASAKLLRELTESGEMANSTDHG
                     ISALSVQAALSDQEEEKL"
BASE COUNT          286 a          401 c          358 g          318 t
ORIGIN      
        1 cggcagtctc gcagtgattt acagcttctc gattgtaccg tatttcgggc tgtgagtaat
       61 ctggttgaaa attggttttg caacaggctc tattctagaa tattttcggg aaaagggata
      121 cctccaacta cccggcccgc aaaggagacg tgcgtcatgg caggaaaagg aaaaaaggag
      181 cgcatttcag tggaagcgga tgcttctccc ctcgggattc ccctgggtgc cctcctgggg
      241 agggaaaccc cgccggagga cccccccgcc gggaaggtac gccccgaagc agtcccttcc
      301 actgctgaag ggaaactgcc cggacgggcc gtcctttcca gggagacgaa gggacgggga
      361 ggcaagaccg tcacccgcat ttcctttcgg gacggaactc cgccggacac ggcggcgctg
      421 gcgaaaacac tccgcacggc catgggctgc ggggggaccg tggagggaga cgacatcctg
      481 cttcagggcg accagatcga acggaccgcc gaatggtttg ccgcccgaag ggtgaaggtc
      541 acgagggcac actgacgtct ccctgacgtt cgaattctac agtttttcct cctcctgatc
      601 tgacagggct gcctgcacgg agagcgctga tattccatga tccgtactgt tcgccatctc
      661 tccgctctcg gtcagctccc taagcagctt ggccgaagcg gactctacca ccgtaccgca
      721 cttgccgcag acggaggctc ccgacgcgag agtggccccg caattgctgc atgtgccgtt
      781 gttttcggtt gatgcctcgc ccgcacctga cgaagctgga gttcccgatg tattctgcgt
      841 ctgcgttgtt ccggacgaag aggtccccga agcgttttca tgctccttcg agactccgtc
      901 cttttcctcc gaattgatgc gcacgttgta ttgagccatc ccctgcgaaa ttccccggat
      961 ctccattgat attcctatcc cccccttccc tattgaaacg caggacggaa cacttccctc
     1021 cctccggata cgacaccgcg cttcttgttt tcttttcggc taccccgggg aaaaatagca
     1081 tcaagaaaca cagctctttt acgaagaagt gcaaagaaaa acaaagcaat gctctctctt
     1141 ccgtcgccct tgctatactt tgaagagtgt ttggatgcag ggaaaggaag aaaaacaatg
     1201 cgacccattt tacctaaatc cgcaggttcg ttccctcaag taaagggata gttccccgga
     1261 cggggaaatg aactgttttc gtttctccgt tccgtttctt tatttccctt ttcaggtatt
     1321 tcaggtcttt ccccttttgc gggtacactc catctgcacg gat
//
LOCUS       DAOTHD010000714         1361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_115717_length_1361_cov_3.013017, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000714 DAOTHD010000000
VERSION     DAOTHD010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115717_length_1361_cov_3.013017"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(242..583)
                     /locus_tag="P5219_10740"
     CDS             complement(242..583)
                     /locus_tag="P5219_10740"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRX27285.1"
                     /translation="MKTFDMETVEKFTGRPGFIGKFIHSEGFTFAQWEIKKGTELVFH
                     DHPHEQTTLLLAGKMELGTADKKVTLTPGQGIVFAGGEAHGGFALEDCLVIDVFCPVR
                     EDFKKLQQEQK"
     gene            972..>1361
                     /locus_tag="P5219_10745"
     CDS             972..>1361
                     /locus_tag="P5219_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRX27286.1"
                     /translation="MRKGILHSYERFFPLTDRTPRLSLCEGDTPLVPLRNIGRELGVE
                     LYGKYDGLNPTGSFKDRGMVLAVSKALEEGAKALICASTGNTSASAAAYGAAAGIPCF
                     VLLPAGKVAVPTGPPREFPASSCCRRGR"
BASE COUNT          250 a          384 c          430 g          297 t
ORIGIN      
        1 tttcgctcag gatgactagc aagggcggga tccctcgcat tcgttcggga tgacaacccc
       61 ttctgtcatc ccgaacgagc gtagcgagga gggacctgca tttgattctg tactgcctca
      121 agaccgagat tccccccctc cggggaggct tctcgatccg gcctcgccgt tcggaatgac
      181 caaaatagga gcgagatcct tcgcttcgct caggatgaca agcaaggacg aggagtcgat
      241 gctatttctg ctcctgctgc agcttcttga agtcctcccg cacggggcag aagacgtcga
      301 tgacgaggca gtcctccagg gcgaagcctc cgtgggcctc gcctccggcg aagacgatgc
      361 cctgccccgg ggtgagggtg acctttttgt cggcagtgcc gagttccatt tttccggcga
      421 gcagcagggt ggtctgctcg tgggggtggt catggaaaac aagttctgtt ccctttttga
      481 tctcccactg ggcgaaggtg aagccctcgg agtggatgaa tttcccgatg aagcccggtc
      541 ttccggtgaa cttttcgacg gtttccatgt caaatgtctt catgtctgca tgcatccttt
      601 ccatttggta gtgtactcgt cctgcccctc ctggggagaa ctgggcaccc ttgacacaat
      661 ccggtctcgc gagtataagt aggaatcaat ttcgtttcca ataatatgcc atttgcgcga
      721 tgaagggaag agtacctttt tccggcggcg gacagagagg gaggacccag gctgagaatc
      781 ctcctccgga gcgggaaagg gaagaccacc cccgagcggg cgccgaagtc ctgagggagg
      841 gaaggcgccg gggtccgccc catacagcgg gatcgagcag ccgattattt cggaagtgga
      901 gtgggaccgc gatgagcctg catcgtctcc agcatgtgga cgatgcaggc tttttttcag
      961 gagggagatt catgagaaaa ggcattctgc attcctacga gcgttttttt cccctgacgg
     1021 accggactcc ccgtctttcc ctgtgcgaag gcgacactcc ccttgttccg ctgcggaata
     1081 ttggccggga gcttggtgtg gaactgtacg gcaagtatga cggcctgaac cccacgggat
     1141 ccttcaagga ccgggggatg gtacttgctg tgtcgaaggc cctggaggag ggtgcgaagg
     1201 ccctcatctg cgcgtccacg gggaacacgt ccgcctcggc tgcggcctac ggggccgccg
     1261 cgggaattcc ctgcttcgtc ctgctgccgg cggggaaggt ggctgtgcct acggggccgc
     1321 cgcgggaatt ccctgcttcg tcctgctgcc ggcggggaag g
//
LOCUS       DAOTHD010000715         1357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_116164_length_1357_cov_4.046851, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000715 DAOTHD010000000
VERSION     DAOTHD010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116164_length_1357_cov_4.046851"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="P5219_10750"
     CDS             <1..504
                     /locus_tag="P5219_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27287.1"
                     /translation="KGKVKQVRISPATSDNVVTYPVIISVANPDLKLKPGMTANVSIV
                     TDRRKDVLKVPMAALRFSPPPEDAAPQTAAATSSPFSPSMPRRRPGGGAGQANGNGNA
                     GRTGTVSSVWTVRDGVLGEKIQFRAGIGDGSFVEVITSKELKEGDLLAVSFSEQPKES
                     LWGKIFK"
     gene            501..1193
                     /locus_tag="P5219_10755"
     CDS             501..1193
                     /locus_tag="P5219_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX27288.1"
                     /translation="MSLIDVRDIVKIYRTGDVELRALDGVTFTVDRGEFVSIMGHSGS
                     GKSTMMNILGCLDVPDEGSYSLDGSEVGSLPKDALAGIRNRKIGFVFQGFNLLPRATA
                     LENVELPLIYAGVPGKTRKDRGKAALERVGLAERMTHRPSQMSGGQQQRVAIARALVG
                     ETPLILADEPTGNLDTKTSEEIMNLFVELNREGKTIILVTHEPDIAEYSSRTLMFRDG
                     KLTGDERRAPRA"
     gene            1252..>1357
                     /locus_tag="P5219_10760"
     CDS             1252..>1357
                     /locus_tag="P5219_10760"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRX27289.1"
                     /translation="MLGIIIGVAAVITMVAIGAGASVNIQKFISGVGSN"
BASE COUNT          275 a          456 c          417 g          209 t
ORIGIN      
        1 aaagggaagg tgaagcaggt gcgcatctcc cccgccacat cggacaacgt ggtaacctac
       61 cccgtcatca tctccgtagc caaccccgac ctgaagctca agcccggaat gacggccaac
      121 gtgtctatcg tcaccgaccg ccggaaggac gtcctgaagg tccccatggc ggccctgagg
      181 ttttcccctc ccccggagga tgccgctccg cagactgccg ccgccacgtc gtcccccttc
      241 agtccttcca tgccccgacg ccgacccgga ggaggagccg gccaggccaa cgggaacggc
      301 aacgcgggca ggacgggaac ggtgtcctcc gtctggacgg tacgggacgg tgtcctcgga
      361 gagaagatcc agttccgcgc cggcatcggc gacggctcct ttgtggaggt catcacctcg
      421 aaggagctga aggagggtga cctcctggct gtctccttct cggagcagcc aaaggaatcc
      481 ctgtggggga agatcttcaa atgagcctca tcgatgttcg ggacatcgtg aaaatctacc
      541 gcaccggcga cgtggagctc cgggccctgg acggagtgac cttcacggtg gaccgggggg
      601 aattcgtctc catcatgggg cactccggct cgggcaagtc caccatgatg aatatcctcg
      661 gctgcctgga cgtgcccgac gaaggctcct actccctcga cggcagcgag gtggggtccc
      721 tgccgaagga cgccctcgcc ggaatccgga acaggaagat cggcttcgtc ttccaggggt
      781 tcaacctcct tccccgggcc accgccctgg agaacgtgga gctgcccctc atctacgcgg
      841 gggtcccggg aaaaaccaga aaggatcgtg ggaaggccgc cctcgagcgg gtgggcctcg
      901 cggaaaggat gacacaccgt ccaagccaga tgtcaggagg ccagcagcag cgggtggcca
      961 tcgcccgggc cctggtggga gaaacgcccc tcatcctggc ggacgagccc acgggcaacc
     1021 tggacaccaa aaccagcgag gagatcatga atcttttcgt ggagctgaac cgggagggca
     1081 agaccatcat cctggtcacc cacgaacccg acatcgccga atacagctcc cggaccctca
     1141 tgttccggga cggcaaactc accggcgacg aaaggagggc tccccgtgct tgagaccctc
     1201 cgcaccgccg tgcgctccct gctctccaac aaaatacggt cggccctcac catgctgggc
     1261 atcatcatcg gcgtcgccgc agtgatcacc atggtggcca tcggcgccgg ggccagcgtc
     1321 aacatccaga agttcatctc cggcgtgggg agcaaca
//
LOCUS       DAOTHD010000716         1357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_116206_length_1357_cov_2.704301, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000716 DAOTHD010000000
VERSION     DAOTHD010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116206_length_1357_cov_2.704301"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="P5219_10765"
     CDS             complement(<1..542)
                     /locus_tag="P5219_10765"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HRX27290.1"
                     /translation="MASYDPEQLVFEQEVVSSIKPVLGIEDRPKTFLETLFYAWQVTL
                     VDFTPFIWAGMFVTLAGLPENVLPVMISTCFLAMGVGTLIQTTVGNRLPIVQGPSASV
                     LSAMGPVTAMYGFPAMWGSVLVGGLLETLLGFSRVLGKIRKFIPPVVTGSVVATIGFV
                     AARISLTWIFGSGKQLHLILA"
     gene            complement(644..>1357)
                     /locus_tag="P5219_10770"
     CDS             complement(644..>1357)
                     /locus_tag="P5219_10770"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HRX27291.1"
                     /translation="EGVLEAAKTVGRGSEQFAMHVKGVGLNEQGVRSHKAWGFGMAVS
                     ARGGGHLNGSPQTENRQISSWTGQWLFGNENAGVPGSYGGKGRLVAWYEIYKAIVDSV
                     GMCYFTSGWYDPALADIGPLSRALGAFGCEGLTPEELWKRGRWIVEAEKAFNTVHAGF
                     GRKDDILPQRVMEEPLTFGPYAGAVMDRLSFDGMLDEFYRERGWDPETGLQTPEGLME
                     IGAQDIAEYLRKGRWQETL"
BASE COUNT          272 a          432 c          377 g          276 t
ORIGIN      
        1 gcgaggatca ggtgaagctg cttgccgctg ccgaagatcc acgtgaggga gatccttgcg
       61 gcgacaaagc cgatggtggc caccacggaa ccggtcacta cgggagggat gaatttcctg
      121 atctttccga gcaccctcga gaatccgagc agggtttcca gcaggccgcc cacgagcacc
      181 gatccccaca tggcgggaaa gccgtacatg gcagtcacgg gccccatggc ggagagcaca
      241 gaagcggacg gtccctggac gatgggaagt ctgtttccca ctgtagtctg gataagtgta
      301 cccacaccca tggcgaggaa gcatgtgctg atcatgacgg gcagcacgtt ctcgggaagc
      361 cccgcaaggg tgacgaacat tcctgcccag atgaaggggg tgaaatcaac cagggtcacc
      421 tgccaggcgt aaaacagggt ttcgagaaag gtcttcggcc ggtcctcgat gccgagaacc
      481 ggcttgatgg aactgaccac ttcctgttca aatacgagct gttccggatc ataggaagcc
      541 attctctctt ttcctccttg ctattttttc gttggccccg gctgaccggg cagagccagg
      601 atccgggaga agaagccacc gggggaccct tcgcatcgaa atcttacaat gtttcctgcc
      661 atctcccctt ccggagatac tccgcgatgt cctgagcgcc gatttccatc agcccttcgg
      721 gggtctggag gcccgtttca gggtcccatc cccgttcgcg gtagaattca tcaagcatcc
      781 cgtcaaagga caacctgtcc atcaccgccc cggcataggg accgaaggtg aggggttctt
      841 ccatgacccg ttggggcaga atgtcatctt tcctgccgaa ccccgcgtgg accgtattga
      901 aggctttctc cgcctccacg atccaccgcc cccttttcca cagctcttca ggggtgagcc
      961 cttcgcagcc gaaggccccc agggcccggg acaggggtcc aatgtccgcc aatgcaggat
     1021 cataccatcc ggaagtgaaa tagcacatcc ccaccgagtc aacgatggcc ttgtagattt
     1081 cgtaccatgc gacgagcctg ccttttcccc catatgatcc gggaactccg gcgttttcat
     1141 tgccgaagag ccattgtccg gtccaggagg aaatctgcct gttttccgtc tggggcgagc
     1201 cgttgaggtg tcctcctccc ctggcgctta ccgccatgcc gaacccccat gccttgtggg
     1261 accggacgcc ctgctcgttc agcccgacac ccttgacgtg catggcgaac tgttcgctgc
     1321 cccggcccac agttttcgcc gcctcgagaa caccttc
//
LOCUS       DAOTHD010000717         1357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_116232_length_1357_cov_2.393241, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000717 DAOTHD010000000
VERSION     DAOTHD010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116232_length_1357_cov_2.393241"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="P5219_10775"
     CDS             complement(<1..742)
                     /locus_tag="P5219_10775"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HRX27292.1"
                     /translation="MILILGAFDGFHLGHRRLLETAGAMAERLSAKNEGDWGVVTFSP
                     HPGNVVKGREIPVLFTEPERDVLARFLHIPRTLKIPFTPEIAGMSPADFLDYLEELLP
                     VTGIVVGKDFRFGRNREGDTSFLRKRSEAKGWLFEEAEPLSIDGEKVGSSSIRSHVAH
                     GSVALAKRLLGYPFFFEGTVVPGDGRGTRLGFPTANVHYPTEKVMPRRGVYCVSVFVD
                     TVWMPGALNVGVNPTFRQEERGVRAETHI"
     gene            complement(739..>1357)
                     /locus_tag="P5219_10780"
     CDS             complement(739..>1357)
                     /locus_tag="P5219_10780"
                     /inference="COORDINATES: protein motif:HMM:NF013660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27293.1"
                     /translation="RIAALLPSFLGTRLQTPPDISAVRVNGKRAHRIARSGESPVISS
                     RPVHISSIRLTGEEDEGRSFALRIRCRKGTYVRSIVRDIGRALGCGAYVLSLVRRSIG
                     RFRQEDALAFDRLESGDEDLSRNLRPLSELAAQYYSFSCGPGTDAVLLAGKNVPLPGL
                     VPLSPGDGDPGGKAVILGEKLFSWGNLLPDGLFEPKINILLEGNP"
BASE COUNT          294 a          415 c          381 g          267 t
ORIGIN      
        1 taatgtgcgt ttccgcccgt actccccgtt cttcctgccg gaaggtgggg ttcactccca
       61 cgttgagggc ccctggcatc cacacagtat ccacaaacac cgaaacacag tatacccctc
      121 tcctcggcat gaccttttca gtcggataat gcacgtttgc cgtggggaac ccgagcctgg
      181 ttcctcttcc gtctccaggc accacagttc cttcaaagaa aaaagggtac cccagaaggc
      241 gctttgccag ggctacggag ccatgggcaa cgtggctccg aatcgagctg cttccgacct
      301 tctcaccatc aatcgaaagc ggctccgctt cttcgaagag ccaccccttc gcttccgacc
      361 tcttccggag gaaggatgta tcgccttccc tgtttctgcc gaaccggaaa tcctttccca
      421 ccacgatgcc cgtcaccgga agcaactctt caagatagtc caggaaatcc gcaggggaca
      481 tcccggctat ttcaggggtg aagggaattt tcaatgtcct tggaatatgg aggaagcggg
      541 caaggacatc ccgctccggc tcagtgaaga gaaccggtat ttccctcccc ttcaccacat
      601 ttccggggtg gggggaaaat gtgaccactc cccagtcgcc ttcatttttc gcggaaagcc
      661 gctccgccat ggcacccgca gtttcgagaa gccggcggtg accaaggtga aagccgtcaa
      721 aggcaccgag gatcaggatc atggattccc ctccagaaga atgtttatct tcggttcgaa
      781 gagaccgtct gggagcaggt ttccccagct gaacagcttt tctccgagaa tgacggcctt
      841 acccccggga tccccgtctc ccggggagag ggggacaagg cccggcagag gaacgttttt
      901 ccccgcgaga agaacggcgt ccgtcccggg gccgcaggaa aaagaataat actgggctgc
      961 aagttcggac agaggcctga gattccttga gagatcctca tctcctgact cgagcctgtc
     1021 aaaggccagg gcatcttcct gccggaagcg cccgatggac cgccgcacga gggagagaac
     1081 atatgccccg catccaagcg ccctgccgat gtcacggacg atgctccgaa cgtaggtacc
     1141 cttccggcac cgtatgcgga gggcgaaaga gcggccctcg tcctcttccc cggtgaggcg
     1201 gatggacgat atatgcacag gcctggagga aatgacgggg ctttcccctg agcgggcgat
     1261 cctgtgggcc cgtttcccgt tgacccggac tgcggatatg tccggtggag tctgaaggcg
     1321 ggttccaaga aaagaaggaa gaagtgccgc tatcctg
//
LOCUS       DAOTHD010000718         1355 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_116512_length_1355_cov_2.006154, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000718 DAOTHD010000000
VERSION     DAOTHD010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116512_length_1355_cov_2.006154"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="P5219_10785"
     CDS             complement(<1..1346)
                     /locus_tag="P5219_10785"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRX27294.1"
                     /translation="MKGLKIGCVQPRAFDVPEWKEALERALELADDAVAGGAELVLLP
                     EALFPAYYLGGGEDSAASWRKSDPRTVFSGYAAGKGIYLAAGLVLEDEGALYNGAMLW
                     GPDGRELLRTFKSNLWHFDGRYVLPGNSFPLAVTEFGPVGMMVCADGRIPEISRILAL
                     KGAKLILDLTNLTSTGRNRDTLSNPQLEYMLPARAAENGVWIAVADKVGLEAETVLNC
                     GGSRLIDPLGNVVASLGSSAEGVLVAEADLEIPCPPMPERTPKLYSILTAETVSTGAY
                     LNQFDPLSAAEDEVFSALCRFSFSGMEDYLEKAARFLARALDQGNRLVCLPPMEEEGC
                     SEVAEKLSGLLAGSDVVAVLNGPAGEGRDTVVFSANGVIGRFSGDVLDPLDTPAGRIG
                     TVWGDDGWNPEPVRCLMLGGSEAVVWYGDGKRLRDISVKVARTRAAENRIFLLYFHG"
BASE COUNT          270 a          460 c          372 g          253 t
ORIGIN      
        1 ccgtggaaat acagcagaaa aatcctgttc tccgcagccc ttgtcctggc aacttttacc
       61 gatatatccc ggagacgctt tccgtccccg taccagacca ccgcttccga tcctccgagc
      121 atgaggcagc gcaccggctc cgggttccag ccgtcgtccc cccacaccgt cccaatcctt
      181 cccgcaggag tgtccagggg gtcgagcaca tctccggaga aacggccgat aacaccgttt
      241 gcggaaaaca cgacggtgtc cctgccctca cctgccgggc cgttcaggac ggcgacaacg
      301 tcgcttcccg ccaggagccc cgacagtttt tccgcaactt cggagcatcc ttcctcctcc
      361 atggggggca ggcagacaag cctgtttccc tggtccagtg cacgggcaag aaatcgggcc
      421 gctttttcca gatagtcctc catgccggag aaactgaacc tgcacagggc ggagaaaact
      481 tcatcctccg cagcggacag gggatcgaac tgattgagat aggctcctgt ggaaaccgtc
      541 tccgccgtca gaatggaata aagtttcggg gtacgttcag gcataggcgg acagggaatc
      601 tccagatcgg cctcggccac caggacccct tccgccgatg aaccaaggga cgcaactacg
      661 tttcccagcg gatcgatcag gcggctgccg ccgcagttga gcactgtttc cgcctcaaga
      721 cccaccttgt cggccacggc gatccacaca ccgttttccg ccgcccgggc gggcagcatg
      781 tactcgagct ggggattgga cagggtatcc ctgtttcgcc cggtggaagt aaggttcgta
      841 aggtcgagga tcagtttcgc ccccttcagg gcgagaatac ggcttatttc gggaatcctt
      901 ccgtcggcgc acaccatcat gcctaccggc ccgaattccg taaccgccag aggaaaggag
      961 tttccgggaa ggacataccg tccgtcgaaa tgccagaggt tcgacttgaa ggtcctcagc
     1021 agctcccgtc cgtccggtcc ccagagcatt gccccgttgt acagggctcc ctcgtcctcc
     1081 agcacgagcc ccgcagcgag ataaatcccc ttcccggcgg cgtatcctga aaaaaccgtc
     1141 cgggggtcgg acttccgcca cgatgctgcg gagtcctccc cgcctccaag gtagtaggcg
     1201 gggaagaggg cctccggcag gagcacgagt tccgcacctc ccgccacagc atcgtccgcc
     1261 agttccaggg cccgttcaag ggcttctttc cactccggca cgtcaaaggc gcggggctgg
     1321 acacacccga tcttcaggcc tttcaccttc cttca
//
LOCUS       DAOTHD010000719         1352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_116881_length_1352_cov_2.309946, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000719 DAOTHD010000000
VERSION     DAOTHD010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116881_length_1352_cov_2.309946"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1352
                     /locus_tag="P5219_10790"
     CDS             <1..>1352
                     /locus_tag="P5219_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRX27295.1"
                     /translation="VRPIAASLREFFGSGQLSQFMDQTNPLAEVTHRRRLSALGPGGL
                     SRERAGFEARDVHYTHYGRVCPIETPEGPNIGLVTSLATYARLNEYGFLITPRRKVEN
                     GRVTGEIVFLSADEEDGVYVGMANTPVDADGRITEEQCFTRYQGNIVTVPREEVTYLD
                     VSPKQIVSASTALIPFLEHDDANRALMGSNMQRQAVPLIAPEAPVVGTGVEHRIAKDS
                     GSCVVAMKDGVVEYVDSTRIEIRKGAESHIYPLVKFRRSNQGTVIHQKPIVHKGETVE
                     KGQIIADGQASEGGELALGRNVIVAFMPWEGFNFEDAILLSERLVKEDFYSSIHIEEY
                     EIEARDTKLGPEEITRDIPNVGEDMLKNLDEDGIVRVGAEVNAGDILVGKVTPKGESD
                     QTPEEKLLRAIFGEKAREVRDTSLKVPHGARGKIVAVKQMTRADSPDALSPGVNKVVK
                     "
BASE COUNT          288 a          436 c          416 g          212 t
ORIGIN      
        1 cgtgcgcccc atcgctgcct ccctccggga gttcttcggc tccggccagc tctcccagtt
       61 catggaccag acgaaccctc tcgccgaggt gacccacagg cgcaggcttt ccgcccttgg
      121 tcccggaggg ctttccaggg aacgggccgg gttcgaggcg cgggacgttc actataccca
      181 ctacggccgg gtctgcccca tcgagacgcc tgaaggtccc aatatcggcc tcgtgacctc
      241 ccttgccacc tatgcccgcc tcaacgagta cggattcctc atcaccccca ggcgcaaggt
      301 ggaaaacggc agggtgaccg gggagatcgt cttcctgtcc gccgacgagg aggacggggt
      361 gtacgtgggc atggccaaca ctcccgtgga cgccgacggc agaatcaccg aggagcagtg
      421 cttcaccagg taccagggca acatcgttac ggtgccccgg gaggaagtga cctacctcga
      481 cgtctccccc aagcagatcg tctccgcctc cacggcgctg attcccttcc tggagcacga
      541 cgacgccaac cgggccctca tgggctcgaa catgcagcgc caggcggtac ccctcatcgc
      601 ccccgaggcc cccgtggtgg gcacgggggt ggagcaccgc atcgccaagg attcggggtc
      661 ttgtgtcgtg gccatgaagg acggtgtggt ggagtacgtg gattccaccc ggatcgagat
      721 ccgaaagggt gccgaatcgc acatttaccc ccttgtgaaa ttccgccggt ccaaccaggg
      781 cacggtgatc caccagaaac ccatcgtcca taagggggaa acggtcgaaa agggacagat
      841 catcgcagac ggccaggcct ccgaaggagg agagctcgcc ctcgggagaa acgtcatcgt
      901 cgctttcatg ccctgggaag gcttcaactt cgaggatgcc atcctgctca gcgaacggct
      961 tgtgaaggag gatttctatt cctccatcca tatagaagag tacgagatcg aggcccggga
     1021 caccaagctc ggccccgagg aaatcaccag ggatattccc aacgtgggcg aggacatgct
     1081 gaagaacctc gacgaggacg gcatcgtccg cgtgggcgcc gaagtcaacg ccggcgacat
     1141 cctcgtcggg aaagtcaccc ccaagggaga atccgaccag acgccggaag agaagcttct
     1201 tcgggccatc ttcggagaaa aggcccggga agtccgggac acgtccctca aggtgcccca
     1261 tggagcacgg ggcaagatcg tggcggtaaa gcagatgacc agggccgaca gccccgacgc
     1321 actcagcccg ggcgtaaaca aggtggtcaa ga
//
LOCUS       DAOTHD010000720         1352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_116887_length_1352_cov_2.176561, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000720 DAOTHD010000000
VERSION     DAOTHD010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116887_length_1352_cov_2.176561"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..628
                     /gene="thiW"
                     /locus_tag="P5219_10795"
     CDS             107..628
                     /gene="thiW"
                     /locus_tag="P5219_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HRX27296.1"
                     /translation="MKSLLKGNGKARIRKLTFAGLLVAAGILLSGVSFPMGPTRCYPF
                     QHTVNVLAGAALGPWWAAGAAFVTSLVRNMTGTGTLFAFPGSIPGALAAGLAFRYFHS
                     SWAALAEPLGTGFLGAAVSAWILGPAVGKTAGLSVLMTAFLASSVPGAVLGMVLVHVL
                     GKTSLSPFFQGGK"
     gene            711..>1352
                     /locus_tag="P5219_10800"
     CDS             711..>1352
                     /locus_tag="P5219_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27297.1"
                     /translation="MTAVQQIIPLNILKCYLSIRRSKTEKITAFFYEILSPAGVCTSS
                     IGFAGSGEDDETAVSVESEIRDFIAHTETLSGRRKETGKKLPATLLASLEGILGEVLT
                     PEAVRESLDKGEGGNRTLEERIREEMAARLGYRATAYIKTERKSSSELEDLLGPDFLA
                     GGTAAAEQDESGGAISFLIPCTPSIDPVRGVPAGALEKGDYIVVTLPSGESPLR"
BASE COUNT          285 a          386 c          404 g          277 t
ORIGIN      
        1 acgaggctct ggttcggccc ggacaccagg ctcgacgccg cccgctggaa aacctttgcg
       61 gatttcgctc ttcagtgggg aatgatcgaa aaggaagtga acgtctatga aatcgttgct
      121 gaaaggtaac gggaaagccc ggattcgaaa gcttaccttc gcggggctgc ttgtggcagc
      181 cgggattctt ctgtcagggg tttcctttcc catggggccc acacggtgct accccttcca
      241 gcacacggta aacgttctcg ccggagccgc cctgggtccg tggtgggccg ccggggcagc
      301 cttcgtcacc agtctcgtcc ggaacatgac gggaacgggc actctcttcg ccttcccggg
      361 gagcatcccc ggcgccctgg cggcaggact ggccttcagg tattttcatt catcatgggc
      421 agcccttgcg gaacctctcg gaacgggatt tctcggagcg gccgtttcag cctggattct
      481 cggccctgcc gtcggaaaga ccgcaggact gtcagtgctt atgactgcct ttctcgcgag
      541 cagcgttccc ggggcggtcc ttggcatggt gctggtccac gtgctgggga agacatccct
      601 ctccccgttt ttccagggag gaaaataacc cggcggaaat tacccttcac cgggagaaca
      661 aactccttta taatccttgt actacgcgcc atgaagagga gattttccag gtgaccgctg
      721 tacagcaaat cataccgttg aatattctga agtgctatct ttccatcagg agatccaaga
      781 cggaaaagat cacggctttt ttctacgaaa tcctctcccc ggccggagtc tgcacttcta
      841 gcataggatt tgcaggctca ggggaagatg acgagacggc cgtttctgta gaatcagaga
      901 tcagggactt tatcgcccat acggaaacac tgtcgggccg ccggaaggaa acggggaaaa
      961 aacttccggc gaccctcctt gcatctcttg agggaattct cggggaagtg ctcactcccg
     1021 aagcggtgag ggaaagcctc gacaaggggg aggggggaaa caggaccctg gaggagcgga
     1081 tcagggaaga gatggccgcc cgcctcggct acagggcaac ggcatacatc aagaccgaaa
     1141 gaaaaagctc ctcggaactt gaggacctgc tcggcccgga tttccttgcc ggtgggacgg
     1201 ctgcggcaga acaggacgaa tccggcgggg ctatcagttt cctgattccc tgtactccca
     1261 gtatcgaccc cgtacgaggc gtgccggcgg gagccctgga aaagggagat tatattgtcg
     1321 tcactctccc ctccggggag tcacccctca gg
//
LOCUS       DAOTHD010000721         1351 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_116936_length_1351_cov_3.881944, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000721 DAOTHD010000000
VERSION     DAOTHD010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116936_length_1351_cov_3.881944"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="P5219_10805"
     CDS             complement(<1..942)
                     /locus_tag="P5219_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRX27298.1"
                     /translation="MKKIVALLIAVAVLCSVSVPAFAAYKDEYKLDIVPSLTTAWGMG
                     AQYFADLVKERSGGKINIKVYPGSQLTTGKQTNAFLLVRNGTIDFAVQSSINYSPQLV
                     ELNLFALPFFIANQPDRYAALDAVKAGKAGEMVIKAIEDKGVKFLAFGENGFRELTNS
                     KKAIRTPDDLTGMKIRVVGSPLFLDTFRALGANPVNMNWSDTMAAIQQGVVDGQENPI
                     STFYPVKMHDYHKFMTDWHYVVDPTLYVVNPAVWKSFSAEDQKMIQAAAVEAGKYMIG
                     LARIGLDDGTAQKYLESIGKVPEITDWYGTLKSVGMEV"
     gene            complement(1065..>1351)
                     /locus_tag="P5219_10810"
     CDS             complement(1065..>1351)
                     /locus_tag="P5219_10810"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HRX27299.1"
                     /translation="AVYLEPLRMEGCIELITVAGIIGEDDRGELSVHLHGVLAGSDMK
                     PVAGHLADRGENRVLATAEVVINGFSGAEFVRSFDEETGFVLFKVRPTGP"
BASE COUNT          251 a          390 c          417 g          293 t
ORIGIN      
        1 cacttccatg ccgacgctct tcagggtgcc gtaccagtcg gtgatctcgg gaaccttgcc
       61 gatgctctcg aggtacttct gggcagtgcc gtcatcgaga ccgatgcggg cgaggccgat
      121 catgtacttg cccgcttcca cggcggcagc ctggatcatc ttctggtcct cggcggagaa
      181 ggacttccat acggcgggat tcacaacgta cagggtggga tcgaccacgt agtgccagtc
      241 ggtcatgaac ttgtggtagt cgtgcatctt cacgggatag aaggtgctga tggggttctc
      301 ctggccgtca acgacgccct gctggatggc cgccatggta tcgctccagt tcatgttcac
      361 ggggttggcg ccgagagccc tgaaggtatc gaggaacagg gggctgccca caacccggat
      421 cttcatgccg gtgaggtcgt cgggggtcct gatggccttc ttggaattgg tgagctcacg
      481 gaatccgttc tcgccgaagg caaggaactt cacgcctttg tcctcgatgg ccttgatgac
      541 catttcgccg gcttttccag ccttgacggc gtcaagggcg gcataccggt cgggctggtt
      601 ggcgatgaag aaaggaaggg caaagaggtt cagctcgaca agctggggcg aatagttgat
      661 ggaagactgg acggcgaagt cgatggtgcc gttccggacg aggaggaaag cgttggtctg
      721 ctttccggtg gtgagctggg atccgggata gaccttgatg ttgatcttgc cgccgcttct
      781 ctctttcacg agatcggcga agtactgggc gcccattccc catgcggtgg tcaggctcgg
      841 cacgatgtcg agcttgtact cgtccttgta ggcggcaaac gccggcacgg acaccgaaca
      901 caggactgca acagcgataa gcagtgctac gatctttttc attccctcaa cctcctgagt
      961 atgtggtatg aaaccttctc ttgactacag cgcgttgtgg tgcttgttcg ccctacggga
     1021 ccgatcacct cccgccgcaa caggatgacc cccgcccggc ccgctcaggg accggtgggg
     1081 cggaccttga acagcacgaa ccctgtttct tcgtcgaaag agcggacgaa ttccgctccg
     1141 gaaaaaccat tgatcacaac ttccgcggtg gcaagcaccc ggttttctcc cctgtcggcg
     1201 agatgtcccg ccacgggttt catgtcgctt cccgcgagaa cgccgtgcag gtggacggag
     1261 agttctcccc tgtcatcctc accgataatg cccgcgacgg tgatcagttc gatgcagccc
     1321 tccatgcgga gcggttcgag gtagaccgcc c
//
LOCUS       DAOTHD010000722         1351 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_116982_length_1351_cov_2.682870, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000722 DAOTHD010000000
VERSION     DAOTHD010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116982_length_1351_cov_2.682870"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /locus_tag="P5219_10815"
     CDS             complement(<1..1332)
                     /locus_tag="P5219_10815"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HRX27300.1"
                     /translation="MKRTRTGFLFLLSFLFLLPGVSGAGVESRINSLSQEEKVGQIMM
                     CYFEGPSLSANLKRMIADLKIGGVILYSSRGNIVSTEQTADLCRSLRSFASSSGAWPL
                     FIGIDQEGGLIQRITEGVTRFPGNMALGAAQDEMLAAEAAAVMARELSALGINLNFAP
                     VADVNNNPFNPVIGVRAFGSSPELVSRLSGAMASAFAKEGVIPSAKHFPGHGNTETDS
                     HSGLPLISSTREELEKTEFPPFRTLVASGVPMVMTAHVLVPALDPELPATLSPSILGM
                     LRKEMGFSGVIITDSMGMGALKKGRTIADAAVEAFNAGADILLFGADRGYEEQEHFVI
                     YEALLEACRNGTIPEQRLDDAVEKILDLKTEAGLFREDKERHNARVPCPEGEMIAHRV
                     AAESVTLVRSWREASEKIKMGEKVPLVWPEEQKAAAEILVGVCPFFDLITLP"
BASE COUNT          293 a          383 c          363 g          312 t
ORIGIN      
        1 cggcagggta ataaggtcaa agaacggaca gactcccaca agaatttctg ctgcggcttt
       61 ctgttcttcg ggccagacca gcgggacctt ttcccccatt tttatttttt cggaagcctc
      121 cctccagctg cggaccagcg taacgctttc ggctgcgact ctgtgtgcga tcatttcccc
      181 ttcaggacag ggaacacggg cattgtgcct ttctttgtcc tccctgaaga gaccggcctc
      241 tgtcttgaga tcgagtattt tctccacagc gtcatcaagc ctttgttctg gaattgttcc
      301 attccggcat gcttccagga gtgcttcata aatgacgaaa tgctcctgtt cctcgtaccc
      361 cctgtccgct ccgaaaagaa gaatatccgc accggcgttg aacgcttcta cggccgcatc
      421 ggcaatggtc cttccctttt tcagggcccc catacccatg gagtccgtga tgataacgcc
      481 tgaaaagccc atttcctttc tgagcatgcc gaggatggaa ggggagaggg ttgccggcag
      541 ttcagggtcg agagccggta caagcacatg ggcagtcatg accatgggca ctcctgacgc
      601 cacaagggtg cggaagggcg gaaattctgt tttctcaagc tcttctctcg tggaggaaat
      661 caggggcagc cccgaatgag agtctgtttc cgtgttgccg tggcccggaa aatgttttgc
      721 cgacgggatg actccttctt ttgcaaaggc tgacgccatc gccccggaga gtctggatac
      781 aagttcagga gaggaaccga aggcgcgcac accgatgacg ggattgaagg ggttgttgtt
      841 cacgtccgct acgggagcga aattcagatt gatgcccagg gcggaaagct cccgcgccat
      901 cacggccgct gcctccgctg cgagcatttc atcctgagcc gctccgaggg ccatgttgcc
      961 cgggaaacgg gtgactccct cggtgatacg ctgtataagt cccccttcct ggtcaatccc
     1021 gataaacaga ggccatgcac cggaagacga agcaaaagag cggaggctcc ggcacagatc
     1081 tgctgtctgt tccgttgaca cgatgtttcc cctgctcgaa tagaggatca ctccccctat
     1141 cttcaggtcg gcgatcatcc ttttgaggtt tgccgatagt gacggcccct cgaaatagca
     1201 catcatgatc tgtcccactt tttcttcctg cgagaggctg ttgattctgc tttcaacccc
     1261 cgccccggaa acaccgggga gaagaaaaag aaaagaaaga aggaaaagaa aaccggtacg
     1321 ggtccgtttc attccgaggc tcctccaagg t
//
LOCUS       DAOTHD010000723         1350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_117153_length_1350_cov_1.905792, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000723 DAOTHD010000000
VERSION     DAOTHD010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117153_length_1350_cov_1.905792"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>409)
                     /locus_tag="P5219_10820"
     CDS             complement(<1..>409)
                     /locus_tag="P5219_10820"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRX27301.1"
                     /translation="ILFAFIAVLEDSGYMARGAFVMDRVMHLMGLHGKSFIPMLMGFG
                     CNVPSMMATRILERPRDRMITLLILPFMSCSARLPVFVLFSGAFFGPHAGTAVFSLYI
                     LGIVVAVGAAKILGSTLFKGESSQLVMELPPYHI"
     assembly_gap    410..419
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<420..>1350)
                     /locus_tag="P5219_10825"
     CDS             complement(<420..>1350)
                     /locus_tag="P5219_10825"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HRX27302.1"
                     /translation="LPGIYSLGAASLDEEIAADFLLRSRPDLAIVVADGSNLERSLYL
                     AVQMLETGTPLVLALNMMDAAAEKGITIDVEKLSALLGIPVVPTVARRKEGIEDLKKR
                     VLDELKNRTAPLNSFALPYGERLGPLFDEMEAFLTTKPELIPGLPARTAAVKFTEGDS
                     VVLAAAESAGLLPALESILSGSGAAVESALGYDLQTAVIERRWGFVSGVAAEAVTRDL
                     SLKTRLSLSDRIDRVVTSRTLGLPLFFLVTWAIFRLTYALGDPLVEIFGGLFESLGER
                     SAGFLAAAGLSDAMVSFIQDGLIGGVGSVVVFFP"
BASE COUNT          274 a          416 c          438 g          212 t
ORIGIN      
        1 gatgtggtag gggggcagct ccatgacgag ctgggacgat tctcccttga agagggtact
       61 ccccagtatc ttggcagccc cgacggccac cacgatcccc aggatgtaga gggagaagac
      121 ggccgttccg gcgtgagggc cgaagaaggc gccggagaag agaacgaaca ccggcagccg
      181 ggccgagcag ctcatgaagg ggaggatgag cagggtgatc atcctgtccc ggggccgttc
      241 gaggatccgg gtggccatca tggacggcac gttgcacccg aagcccatga gcatgggaat
      301 gaagctcttg ccgtggagcc ccatgagatg catgaccctg tccatgacaa aggcgcccct
      361 ggccatgtac cccgagtcct cgaggacggc gatgaaggcg aagaggatgn nnnnnnnnng
      421 ggaagaacac caccacggat cccacgcccc cgatgaggcc gtcctggatg aaggagacca
      481 ttgcatcgga gagtccggcg gcggccagga atccggcgga ccgttcgccc agggattcga
      541 agagtcctcc gaagatctcc accaggggat cccccagggc ataggtgagc ctgaagatgg
      601 cccatgtcac cagaaagaac aggggaaggc cgagggtcct cgacgtcaca acccggtcga
      661 tcctgtcgga aagggacagc cgtgtcttca gcgagagatc ccgggtcacc gcttcggcgg
      721 ccacgccgga gacgaaaccc cagcgccgct cgatcaccgc cgtctggagg tcatatccga
      781 gggcggattc caccgccgcg ccgctgcctg agagaatgga ctccagggcg ggcaggagcc
      841 ctgcggactc cgccgccgcg agaaccacgg agtctccttc ggtgaacttc accgccgccg
      901 tcctggcggg aagcccggga atcagctccg gtttcgtcgt cagaaaggcc tccatctcgt
      961 cgaaaagagg accgagccgc tccccgtagg gcagggcaaa ggaattcaga ggcgccgtcc
     1021 tgtttttcaa ctcatcgagc accctcttct tgaggtcttc gattccctcc ttcctccggg
     1081 ccaccgtggg gaccaccgga attccgagga gagcggacag tttttcaacg tctatggtga
     1141 tccccttttc cgccgccgcg tccatcatgt tcagggccag gacaaggggc gtgccggtct
     1201 ccagcatctg gacagccagg tagaggcttc tctcgaggtt cgatccgtcg gccacgacga
     1261 tggccaggtc gggcctgctc cggagaagga aatccgccgc gatctcctcg tcgagtgagg
     1321 cggcgccgag actgtatatc cccgggaggt
//
LOCUS       DAOTHD010000724         1347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_117491_length_1347_cov_3.288700, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000724 DAOTHD010000000
VERSION     DAOTHD010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117491_length_1347_cov_3.288700"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="P5219_10830"
     CDS             complement(<1..342)
                     /locus_tag="P5219_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HRX27303.1"
                     /translation="MKKSFVFVLGVMMVLLVASGALAEAKFHIGICTGTVSQSEDDLR
                     GAETMIAKYGDVSSGGMIKHITYPDNFMQEMETTISQIASLADDPLMKVIIVNQAIPG
                     TTEGFRRVREKR"
     gene            429..788
                     /locus_tag="P5219_10835"
     CDS             429..788
                     /locus_tag="P5219_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27304.1"
                     /translation="MLSPVTSSPEKIESFPGTAGSKKTQHHCNPYTAANKYGLERRGT
                     ATAGKRQNKESRRALVSARLNINHKPPHSRKCFHPTASIIFSKKGEIFPCKKMTDSVP
                     WELNRGLKTTIHAYLRV"
BASE COUNT          383 a          348 c          284 g          332 t
ORIGIN      
        1 ccttttctcc cgtaccctgc ggaaaccttc cgttgttcct ggaatagcct ggttgacgat
       61 gatgactttc atcaggggat catcggcaag ggaggcaatc tgggaaatgg ttgtttccat
      121 ctcctgcatg aaattatcgg ggtacgtaat gtgcttgatc attcccccgc tggaaacatc
      181 cccatatttc gcaatcattg tctcagcgcc gcgaaggtcg tcttccgact gtgacacggt
      241 acctgtgcag atcccgatgt ggaacttagc ttcagccagt gctcctgaag ctaccaacag
      301 aaccatcatg actcccagaa cgaaaacaaa tgactttttc atgaatttcc ctccttaaag
      361 gataagacgg ccagcggcca aaaccgccac aatttcccat gcaggaacgc tcgacaccct
      421 atggacagat gctctccccg gtcacctcct ctcctgaaaa aatcgaatcc tttccaggta
      481 ccgccggatc gaagaaaaca cagcatcatt gcaatccgta cactgcagca aataaatacg
      541 gtctggaaag acgcgggaca gcaacggcgg gaaaaagaca gaacaaagag agcagaaggg
      601 cgcttgtttc ggccaggctg aatataaacc ataaaccgcc acattcgaga aagtgttttc
      661 atcccactgc ctccatcata ttttcaaaaa agggggaaat ttttccatgc aaaaaaatga
      721 ctgattcggt gccctgggag ctaaacaggg gccttaaaac aacaattcac gcatatctta
      781 gagtgtgaat tttaatatat tttttgctaa catttctttc ttatatagca ggattcaata
      841 aaaaaatact cctcagcctg ttttttgtca aacccgcatt ccccaaaatt tcatgcatga
      901 cctgcactcc atttcgtttc caagaggctc acattatgga aatctcggcc ggcacacttt
      961 ccgccctcgg acgaactgta tttaccagaa aatactaacg gaaccttcct ttatttcata
     1021 tttttcttcg tattgacaaa tcatcaatct tccggataat agatatgatg taatggaggg
     1081 aagatcttga gtggaccccc ggaacggggt ccactctttt ttgagggcga aagacggagg
     1141 cgtcgatgcc cttcccttct ttcgaatctc aaggaggaac agaaattaat gaatgacaac
     1201 ggattcgcga gctacgaact gcgggaagaa ctgcttctcg ctctgaaaaa gaaaggattc
     1261 tccatcccta ccccggtaca ggaaaaagtc ctagcccttg acaatttcga gaccgacctc
     1321 atcgtccggg ccaagacggg ctcggga
//
LOCUS       DAOTHD010000725         1339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_118588_length_1339_cov_2.640966, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000725 DAOTHD010000000
VERSION     DAOTHD010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118588_length_1339_cov_2.640966"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="P5219_10840"
     CDS             complement(<1..193)
                     /locus_tag="P5219_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27305.1"
                     /translation="MRKHLLLALMIVVTLLTAAGFLFSLYAFRAQEEAMDSMMRSYVL
                     DLADTFSPGIHFTGRGQMQR"
     gene            complement(190..840)
                     /locus_tag="P5219_10845"
     CDS             complement(190..840)
                     /locus_tag="P5219_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRX27306.1"
                     /translation="MIRILLADDHPLTRSGLAAWLEKEEGVELAALASDGESAWQSIL
                     ELRPDLALLDIEMPGAGGIDIAARIRKNGLKTNVLMLTAYSAHQYVMASLRAGAKGFI
                     LKSAPLEELRKAVFDTARGVFYLDPSVSLTGADEPGEELSPREREVLILAGQGLPASE
                     VASLLSITERTVAAHLTSIYGKLGARNKTEAILLALKKGIVLLDELRFLDGERGTP"
     gene            1028..>1339
                     /locus_tag="P5219_10850"
     CDS             1028..>1339
                     /locus_tag="P5219_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27307.1"
                     /translation="MKKMNGTIAAVVCAAVLLTAGSALAWGGRGGKAMGGQQGMMNQQ
                     NAQTQFQPGQRMMNPGTNPRMSGPMANRQMTGRRGNFGGRCVAGNQQQMMGRRGNFGN
                     SG"
BASE COUNT          276 a          399 c          429 g          235 t
ORIGIN      
        1 gtctctgcat ctgccctctc ccggtaaagt ggatccccgg cgagaaggtg tcagcgaggt
       61 caaggacgta ggagcgcatc atggagtcca tggcctcctc ctgggcacgg aaggcataca
      121 gagaaaaaag gaatcctgcc gcagtgagga gagtgacgac aatcatgagc gcaagaagaa
      181 gatgctttct catggcgtcc ccctttctcc atccaggaac cggagttcgt ccagcagcac
      241 gattcccttc ttcagcgcca gcaggatggc ttccgtcttg ttccgggcgc cgagctttcc
      301 gtaaatagac gtcaggtggg cagccaccgt ccgctcggtg atggagagga gagacgccac
      361 ttcggaagcg ggcagcccct gcccggcaag gatgagtacc tccctctccc tcggggaaag
      421 ttcttccccc ggctcgtcgg ctccggtgag tgacacggag gggtcgaggt agaagactcc
      481 ccgggccgtg tcgaataccg ccttccgcag ttcctccagg ggggctgact tcaggatgaa
      541 gcccttcgcc cctgcccgga gagaagccat gacatactga tgggcgctgt aggccgtgag
      601 catcagtacg ttggtcttca ggccgttttt ccgtatccta gcggcgatgt cgatgccgcc
      661 ggcgcccggc atttcgatgt ccagcagggc caggtccggc cgaagctcca ggatggactg
      721 ccaggcggac tccccgtcgg aggcgagcgc ggccagctcc accccttcct ccttttccag
      781 ccaggcagcg agtcccgacc gcgtgagggg gtgatcatcg gcaagcagaa tccgtatcat
      841 gttccttcct ccttcccgac agcgctttcc ctgtccgggg aagcgatccc atgggccgat
      901 tgtagcatga cggaccggac cggaattgtc atttcttaca atagggaaac cggcagatcc
      961 gaccatgcgg ccttcccccg gggagagtat cctttaccca tcacaagctg aaaggcggga
     1021 ttgaacgatg aagaaaatga acggaacgat agcggcggtc gtgtgtgccg cagtgcttct
     1081 tacggcaggc agtgcccttg cctggggagg acggggcggc aaggccatgg gcgggcagca
     1141 gggcatgatg aaccagcaga acgcccagac ccaattccag ccggggcaga gaatgatgaa
     1201 ccccgggacc aatccccgga tgtcaggccc catggcgaac cggcagatga cgggacgcag
     1261 ggggaacttc ggcggcaggt gcgttgcagg caatcagcag cagatgatgg gccggagggg
     1321 gaacttcgga aacagcggc
//
LOCUS       DAOTHD010000726         1338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_118726_length_1338_cov_2.670304, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000726 DAOTHD010000000
VERSION     DAOTHD010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118726_length_1338_cov_2.670304"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="P5219_10855"
     CDS             complement(<1..209)
                     /locus_tag="P5219_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27308.1"
                     /translation="MKKLFAVTALVALVVLSAAAAFAGTYAMEKAGIQFTLPDTWEPA
                     DSKLGTMFVSPKGDMVAFFGEVSGGG"
     gene            526..>1338
                     /locus_tag="P5219_10860"
     CDS             526..>1338
                     /locus_tag="P5219_10860"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRX27309.1"
                     /translation="MNSLLIAGGTLWNGNKFTRGEALLLAGGVIAAAGPEKDVRAAAP
                     ASCAELRLSGESVLPGVTDGHIHLTTWAKQRSLLDLSSARSQAEALAMVRAESEKLPP
                     GRWVRGWGYNESRWPEGRSVTKDDLDGLGIPNPVLLQRVCTHLNVADSKALALSGLES
                     ADGVLPERDAIPALRAMERNVFSRSAVKKALKDALFELASHGITCAHPCGADDYGMEE
                     DLSLYGELRDEGSLPLRIFTYHDRHSYPVLPSGLGDGWVHYQGLKIYLDGSLG"
BASE COUNT          310 a          393 c          391 g          244 t
ORIGIN      
        1 ccgccgccgc tgacttcgcc gaagaaggcg accatatcgc ccttcgggga gacgaacatg
       61 gttccgagct tggagtcagc aggctcccac gtatcgggaa gagtgaactg gatcccggcc
      121 ttttccatgg cataggtgcc ggcaaaggcg gcagcggcgg aaagaacgac aagtgcgaca
      181 agagcggtga cggcaaacag ctttttcata aaatggaacc ctcctgagat gtgatgatta
      241 ccaccggatg aacggtgaag aagcctgaat agaactaacg tggatatatt tcgagaaaca
      301 ttattgcaaa aaactaaaaa tatatttgac gcactatctt aatcataaat agtttcaagg
      361 atagggtgtc ttttggaaag aatagcactc ttttgacggg cctgcaatag agtctcagcg
      421 agagaagtgt aaaaaaacat caaggaatga agcgccatta tacctcctcc gaccctataa
      481 taagaatacg gaaacaatgc tgatcatcca agcgggggtg tgtacatgaa cagtctgctc
      541 attgccggag gtactctctg gaacgggaat aaattcaccc ggggggaagc cctgctcctc
      601 gccggaggcg tcatcgccgc cgcgggaccc gagaaggacg tccgtgcggc ggccccggcc
      661 tcctgcgccg aactccggct gtcgggagaa tcggtactgc ccggcgtcac cgacggtcat
      721 atccacctca ccacctgggc aaaacagaga tcgcttctgg acctttcttc cgcccggtct
      781 caggcggaag ccctggccat ggtgagggcc gagtcggaga aactgccccc cggccggtgg
      841 gtgcgggggt ggggctacaa cgaaagccgc tggcccgaag gccgctccgt gacaaaggat
      901 gacctcgacg gtctcggcat accgaacccc gtgctgctcc agagggtgtg cacccacctg
      961 aacgtggcgg acagcaaggc cctggccctt tccgggctgg aaagcgccga cggcgttctg
     1021 cccgaacggg acgccatccc tgccctcagg gccatggaac ggaatgtctt ctcccggtcc
     1081 gccgtgaaga aagccctgaa ggacgccctt ttcgagctgg cgtcccacgg gataacctgc
     1141 gcccatccct gcggcgccga cgactacggt atggaggagg acctgtccct ctacggtgag
     1201 ctccgggatg aaggatccct gcccctccgg atcttcacct accatgacag gcactcctat
     1261 cccgtgctgc cttcgggact gggcgacggc tgggtccatt accaggggct caagatctac
     1321 ctcgacggaa gcctcggc
//
LOCUS       DAOTHD010000727         1338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_118732_length_1338_cov_2.634451, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000727 DAOTHD010000000
VERSION     DAOTHD010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118732_length_1338_cov_2.634451"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="P5219_10865"
     CDS             complement(<1..1053)
                     /locus_tag="P5219_10865"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HRX27310.1"
                     /translation="MSPKGALLLPKGADFAHIEPPGRRNLAATLARQGVDRVAVQERA
                     EQSLDEILDIFRTGKRPSSGDERNLFEATLQFVTSYCKDVLFGSPGNIHVFPLLRIGR
                     FLAESVSANPEILLCLVRERPRDYFLLSHSLSVGLLCAFLAHRLFPEQNEVITCAAVG
                     GLLHDIGKQFVPDAVLNKPGRLTEGEFALIKRHPEVGEALLKRAGIDDPLVLKIVRHH
                     HEKMNGSGYPDGLEGRSISMAARIAAVADAYDAITSERSYKKRLPGYKGISEIIASAG
                     NHLDPLVVRTFVNAFGMYPPGTEVELSDGRCGVVAAPGELSILKPRVCLRTGRDGSHY
                     SHPVLLDLAAEKNLFIQ"
BASE COUNT          296 a          394 c          381 g          267 t
ORIGIN      
        1 ctggatgaac agattctttt ccgccgccag gtccaacagc accggatggg agtaatggct
       61 tccgtctctg ccggtccgca ggcagaccct cggcttcagg atgctcagct cgccgggagc
      121 ggccaccacg ccgcacctgc cgtcggagag ctcaacctcc gtccccgggg ggtacatgcc
      181 gaaggcgttg acgaaggtcc gcaccaccag gggatccagg tggttccccg cgctcgcgat
      241 gatctccgaa attcccttgt acccgggcag acgcttcttg taggaccgtt ccgacgtgat
      301 ggcgtcgtag gcgtcagcca cagccgcaat ccttgcggcc attgaaatgg aacgcccctc
      361 gagcccgtca ggataccccg agccgttcat cttttcgtgg tgatggcgca cgattttcag
      421 gaccaggggg tcgtcgatcc ccgccctttt cagcagcgcc tcccccactt ccggatgtct
      481 tttgatgagg gcgaattctc cttccgtcag ccttcccggc ttgttcagaa cggcatccgg
      541 aacgaactgc ttccctatgt cgtgaagcag accgcccact gccgcgcaag taatcacttc
      601 gttctgttcg ggaaaaagcc ggtgggcgag aaaagcgcag agcaacccga cgctgaggga
      661 atgggagagg aggaaatagt ccctcggacg ttcgcggaca aggcagagca atatttcggg
      721 gttggcggac accgactcgg caagaaaccg gccgatgcgc agcaggggaa aaacatggat
      781 gttgccgggc gaaccgaaga ggacatcctt gcagtagctc gtcacgaact ggagggtggc
      841 ctcgaaaagg ttcctctcgt ccccggagga agggcgcttc ccggtgcgga atatatccag
      901 gatctcatcc agagattgtt cggcccgctc ctggacggca acacgatcca ctccctgcct
      961 ggcgagggta gcggccaggt ttctgcggcc cggaggttcg atatgcgcaa aatcggcgcc
     1021 cttgggaagg agaagagcgc ctttcggaga caccacatcc tctgctatcc ttccgcagaa
     1081 tgtggagaga tcccagacag gaagtcggaa tacgaaggta tcgaacatga aggcccccgg
     1141 ccgatgaatt tccgcgcttc atgagtatag catcctgaac tgcattcaac atgaatgaaa
     1201 gcaccactgt ttctgtgaca aaaggttcag gaaagaagag aaaaagttcc tttaaacctt
     1261 tcgttgcggc ctgcctcttc tgtcttctcc tgtcctttcc gcagggcata taacaaaaag
     1321 aaatattaac ccttgaca
//
LOCUS       DAOTHD010000728         1338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_118758_length_1338_cov_2.257989, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000728 DAOTHD010000000
VERSION     DAOTHD010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118758_length_1338_cov_2.257989"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="P5219_10870"
     CDS             <1..1312
                     /locus_tag="P5219_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27311.1"
                     /translation="GALSLVSRDGRKITLPETGRTSGEAVITLPPGELPDARVTLEIG
                     GDGQDSLTSLPFLLTHPGEASPADIVFLSEGGRDFHAVPVSGQGAVAGEYVYGTAGFE
                     ELNEYGNLVRSYRPAVFSLGDRTLRKAPLGEEGEEWARTDLDFRYTSYCSLGRTVFLA
                     GQSTGGWVYAYDTGEGVLRRLTRLPEELRESNLGSPATAAGGKYFYLAGGFILGQSLD
                     LSDRTIDSVYRLDLETLEWRRWGTLSEKRFAAAAAIEDGKLFVAGGKHYELFTVGTSS
                     STVEIPTSSVNVFSLENGSRQDAVLPFSTWKGTLAFDGERETFLFFGGLVYFGDLSMA
                     SPLAAWTSPDGRGGEWELASPRFPFISGDGGWAFSTGGTCHLLSRGWGDQYDRGYQLF
                     SLPAPPPSPGPVPPDNPSAAGCSGGVSPWGALLLVPLALLLKKQ"
BASE COUNT          244 a          436 c          413 g          245 t
ORIGIN      
        1 tggagccctc tccctcgtct caagggacgg ccggaagatt actcttcccg agaccgggcg
       61 gacctccggc gaagcggtca tcaccctgcc gccgggagaa cttccagacg ccagggtgac
      121 ccttgaaatc ggaggggacg gacaggacag cctgacatcc ctccccttcc tgctcaccca
      181 ccccggagag gcttccccgg cggacatcgt gtttctttca gaaggcggca gggatttcca
      241 tgccgttccc gtgtcagggc agggcgccgt ggcgggagaa tacgtctacg gcaccgccgg
      301 attcgaggaa ctgaacgagt acggcaacct tgtgcgcagc taccggccag ccgttttctc
      361 cctcggagac agaaccctgc ggaaggcccc cctcggggaa gaaggggagg aatgggcccg
      421 aaccgacctc gacttccgct acacgagcta ctgctccctg ggaaggacgg tgttcctggc
      481 ggggcagtcg acaggcggat gggtttatgc ctacgacacg ggagagggtg tgctccgccg
      541 tcttacccgc ctgcccgaag aactcaggga aagcaatctc ggctccccgg ccacggccgc
      601 gggagggaaa tacttctatc tggccggagg cttcatcctg ggccagtccc ttgatctttc
      661 ggacagaacc attgactcag tctaccgcct cgacctggaa accctcgagt ggcggcggtg
      721 ggggaccctg tcggagaagc ggttcgccgc ggcagccgcc attgaggacg ggaagctctt
      781 cgtcgccggc gggaaacact acgaactgtt caccgtcgga acctcctcgt cgacggtgga
      841 aattcccaca tcatcggtga acgtcttttc cctggagaac ggatcacggc aggacgccgt
      901 ccttcccttc agcacgtgga agggaacgct ggccttcgac ggagaacggg agacctttct
      961 ctttttcgga ggattggtat atttcggcga cctcagcatg gcctcgcccc tggccgcatg
     1021 gaccagcccc gacggaaggg gcggtgaatg ggaactggcc tccccccgtt ttccttttat
     1081 ttccggggac ggaggctggg ccttctcgac aggagggacc tgtcatctcc tttcgagagg
     1141 gtggggcgac cagtacgaca ggggatacca gctcttctcc ctgcctgccc cgcccccctc
     1201 accgggtccc gttcccccgg acaatccctc cgcggcgggg tgctccgggg gagtctctcc
     1261 ctggggagcc ctgcttctcg tcccgctcgc actcctgctg aaaaaacaat aaaaaaagcg
     1321 gcccccggga aatttcgg
//
LOCUS       DAOTHD010000729         1337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_118826_length_1337_cov_4.040562, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000729 DAOTHD010000000
VERSION     DAOTHD010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118826_length_1337_cov_4.040562"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="P5219_10875"
     CDS             complement(<1..942)
                     /locus_tag="P5219_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HRX27312.1"
                     /translation="MTIRTAKAVIFLIAGGCAAVAGLLFKEHREYREAVVVSPYITEV
                     KKLSDYSDSVKDSVNDANVYIFDSGVPGGTAVLIGGTHPEEPVANMSAQIFTENVKPL
                     QGKVIVINRINRSASLGTRMGEAYPRFYHVKTPWGTKKWRYGDRVASPLDSWPDPEVY
                     IHYPSGQNLAYMDIRNANRTWPGRPDGLLAERTCFAAMELIRKEKADVVMDFHEAELE
                     YAVENTIVVHEKGQPVAAMASMMLTSQSFDVPIGMEFSPKALHGLSHREIGDHSHAVS
                     YLAEVAEPMLDRIRGITDEELLMSGKDRFVMKAGEHKL"
     gene            complement(959..1129)
                     /locus_tag="P5219_10880"
     CDS             complement(959..1129)
                     /locus_tag="P5219_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27313.1"
                     /translation="MDLIMINSVAMILYYFITAFFLLALAKNFLATKSLQEAALYGII
                     MIPFVLRLLRLK"
     gene            complement(1134..>1337)
                     /locus_tag="P5219_10885"
     CDS             complement(1134..>1337)
                     /locus_tag="P5219_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HRX27314.1"
                     /translation="AWSVMWPLGDCLPPTAVVGRATVMELGYTGDYFRGFVRTSLVPM
                     AAILLLCSLFMVYSSELSGVIGG"
BASE COUNT          276 a          353 c          384 g          324 t
ORIGIN      
        1 gagcttgtgc tctccggctt tcatgacgaa acggtccttt ccggacatga gaagttcctc
       61 gtcggttatg ccccgtattc tgtcgagcat tggttcggca acttccgcga gataggacac
      121 cgcgtgggaa tggtctccaa tctccctgtg ggagagcccg tggagggcct tgggtgaaaa
      181 ctccatgccg atagggacgt cgaagctctg ggaggtgagc atcatcgagg ccatggcggc
      241 taccggctgg cccttttcgt ggacaacgat ggtgttttcc acggcgtatt cgagttcagc
      301 ctcgtggaaa tccataacaa cgtcggcctt ttctttccgg atcagttcca tggcggcaaa
      361 gcatgtcctt tcggcaagaa ggccatccgg ccggccgggc catgttctgt ttgcgttcct
      421 gatgtccatg taggcgaggt tctgtccgct ggggtaatga atgtacactt cggggtcggg
      481 ccatgaatcg agagggcttg caacccggtc accgtagcgc catttttttg tgccccaggg
      541 tgttttgaca tggtagaacc gtggataggc ttctcccatc ctggttccga gagaagcgct
      601 ccggtttatc ctgttgatga cgatgacttt tccctgaagg ggtttcacgt tctccgtgaa
      661 gatttgggct gacatgttcg ctaccggttc ttcgggatgg gttcccccga tgagcactgc
      721 agttcctccc ggaactccgc tgtcaaaaat gtacacgttg gcatcgttta ccgagtcctt
      781 tacggaatcg ctgtaatcgc tgagtttttt cacttccgtg atgtagggac tgacgacgac
      841 ggcttccctg tattccctgt gctccttgaa gagtaaaccg gccacggcgg cacagccgcc
      901 tgcgataagg aagatgaccg cttttgcggt cctgatggtc atggtctctt cctcccttct
      961 acttcagccg caaaagccgg aggacgaagg ggatcatgat gatcccgtaa agagccgcct
     1021 cctgcaggct tttggtggca agaaaatttt tcgcaagggc aagaagaaag aatgccgtga
     1081 tgaagtagta caaaatcatt gcaacggaat tgatcataat taagtccatg ctgtcagcct
     1141 ccgatcacgc cggacagttc gctgctgtac accatgaaaa gggagcacag aaggagaatt
     1201 gcggccatgg ggaccaggga tgtccggacg aagccccgga aatagtcccc cgtgtagccg
     1261 agttccatta cggttgccct gcccacgacc gccgtgggtg gaagacagtc tccaaggggc
     1321 cacatgacgg accatgc
//
LOCUS       DAOTHD010000730         1334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_119241_length_1334_cov_2.850665, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000730 DAOTHD010000000
VERSION     DAOTHD010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119241_length_1334_cov_2.850665"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..>1334
                     /locus_tag="P5219_10890"
     CDS             111..>1334
                     /locus_tag="P5219_10890"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRX27315.1"
                     /translation="MQALLIFFAGLITAFAQMAGVFSVFPFINVAMDPASIQDSPWLT
                     LAYTAFSFSSPREFMAALGIAVVLLVILSNGITALTMWAKTRFVLNRNYTLSNRLLAV
                     YLSRPYAFFLINNTSDLGKNVLSEINQLTSSFLMPLFEAMINILILVVIVFMLFSVNP
                     AVTLVTLGFLGTVYGTLNYFLKVKLKQKGAERMEANKKRFRAATEALSSIKISRVIGA
                     ESFFLDRFAENSRKFADLNVFAKVVGQLPSYLMESIVFGGLILFVVLALVRGGEVSAV
                     VPMVSLYAFAGKRLIPALQQIYSSVTQIYYNKAILDRLYQDLVSDEQPDLFPKEGGEK
                     EELPFRHRIVLENIRFRYPEGERNVIDGLDLEIPKESVVGLVGSTGSGKTTLVDIILG
                     LHIPKEGRMLIDDTPV"
BASE COUNT          299 a          440 c          314 g          281 t
ORIGIN      
        1 aagaacatcc cggcaccgtt gagagaaaac cgacaggatc atcacccgaa actgccgcag
       61 aaaaaaccat agtgcggaag ctctatgaaa tcttcacacc tcaggaacgg atgcaggccc
      121 tgctgatctt cttcgccggg cttataaccg cctttgccca gatggcgggt gttttctccg
      181 tgttcccttt catcaacgtc gccatggacc cggcatccat acaggacagc ccgtggctca
      241 ccctcgcgta caccgctttt tccttctcat ccccacggga attcatggcc gccctgggaa
      301 tcgccgtcgt cctcctcgtc atcctctcga acgggatcac cgccctcacc atgtgggcaa
      361 aaacccggtt cgtcctcaac aggaactaca ccctctccaa ccggctgctg gctgtctacc
      421 tctccaggcc ctacgctttc ttcctgatca acaacacgag cgacctcggc aagaacgtcc
      481 tgtcggaaat caaccagctg accagcagtt tccttatgcc ccttttcgag gcaatgatca
      541 acattctgat acttgtggtc atcgtcttca tgctcttctc cgtaaacccc gccgtcaccc
      601 tcgtcaccct cggcttcctt ggaacggtct acggcacgct gaactatttc ctcaaggtga
      661 aactcaagca aaagggcgca gagcggatgg aggccaacaa aaaacggttc cgcgcagcca
      721 ccgaagccct ctccagcatc aagatcagca gggtcatcgg tgcggagtcc ttctttctgg
      781 accggttcgc cgagaattcc agaaaattcg cggacctcaa cgtgttcgcg aaagtggtgg
      841 ggcagctgcc ctcctacctc atggagagca tcgtcttcgg agggctcatc ctcttcgttg
      901 tccttgccct cgtccgtgga ggtgaagttt ccgccgtcgt gcccatggtc agcctctacg
      961 ccttcgccgg caagcgcctg ataccagcac tccagcaaat ctactcctcc gtcacgcaga
     1021 tttactacaa caaggccata ctcgacaggc tctaccagga cctggtttcc gacgaacaac
     1081 ctgacctctt ccccaaagaa ggtggagaaa aggaagaact acccttccgg caccgcatcg
     1141 ttctggagaa catccgcttc cgctaccctg agggagaacg gaacgtcatc gacggacttg
     1201 acctcgagat ccccaaggaa agcgtggtcg gcctcgtcgg gtccaccgga tcgggaaaga
     1261 ccaccctggt ggacatcatc ctcggactgc atattcccaa agaagggagg atgcttattg
     1321 acgacacgcc cgtt
//
LOCUS       DAOTHD010000731         1334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_119256_length_1334_cov_2.560594, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000731 DAOTHD010000000
VERSION     DAOTHD010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119256_length_1334_cov_2.560594"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="P5219_10895"
     CDS             <1..197
                     /locus_tag="P5219_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27316.1"
                     /translation="TGEEGPGEAGWTVSPGAGEVPRGLSCAGTPVDGSAAGSGAWGVF
                     SGFCTTALSQGRSSACRRNQ"
     gene            complement(842..>1334)
                     /locus_tag="P5219_10900"
     CDS             complement(842..>1334)
                     /locus_tag="P5219_10900"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709743.1"
                     /note="catalyzes a two-step reaction, first charging a
                     glutamine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HRX27317.1"
                     /translation="KFFRLSPGTEVRLKHAYLVTCTEVVRNGDGEITELRCTYDPQSR
                     GGEAPDRRRVKGTLHWVSVPHAVKAQVRLYENLFTLRDMSAMEEGKTFRDYLNPDSLK
                     VLENCFVEPGLAQAGPLETFQFLRQGYFCAAPDSTPERPVFNRTVALKDSWAKEVRKE
                     KAQ"
BASE COUNT          272 a          399 c          383 g          280 t
ORIGIN      
        1 gcacgggaga ggaaggtccc ggggaggccg gctggactgt ctcccccggg gccggggaag
       61 tgccccgggg actttcctgc gccgggacgc cggtggacgg gtctgcggcg gggtccggag
      121 cctggggagt tttttccggc ttctgcacta cggcactgtc ccagggcaga tcctcagcct
      181 gccgaaggaa ccagtagact ccgcctccta tggaagccag aaagaccaga agaatcataa
      241 taaggggaat ggcgctcttc cgatcatcct tacgccgcct cgaccgggct acagcgttga
      301 actcccggaa actcgcctca gtcctgtcgg cgtttgtccg gaaccccctc ccctgttcag
      361 gttcatttcc attgtccgaa aacctggtac tgcgtctctg aaggggctgt tccctggaaa
      421 ccggctcctg tcgggaaacc gccattccgg gacgaatttg aacctgggcc gtcacttcag
      481 ggattgccgt tccgcaggcc ggacagaacc ggaacttggc gcttacctct tccccgcatt
      541 cggggcattt tcttccattt tcccctccgg caggggtaaa accattttca tcaaaataag
      601 ccatatcggg cgaacctcct ctgcgtatat ccgaagattt cccataggga actgcatagt
      661 aatgtttttt tattgtagca cttctcttcg ttcagcttct cagcacttct cggtaaaaag
      721 agaaaggaat atcctccggg gaaataaagc ttgaagaaga accggaaaaa aacggcctga
      781 agcggtacgg taccgcagaa gggcggacgg aaaaagggag ggggcaaccc cctccctgcg
      841 ttcactgtgc cttttccttc cggacctcct tcgcccagga atccttcagg gcgacagtcc
      901 ggttgaatac gggcctttcc ggggtggaat ccggggcggc gcagaaatac ccctgccgca
      961 ggaactggaa tgtctccagc ggtccggctt gtgccagccc gggctcgacg aagcagttct
     1021 ccagcacctt cagggaatcg gggttcaggt aatcccgaaa ggtcttcccc tcctccatgg
     1081 cggacatgtc ccgaagggtg aacaggtttt cgtagaggcg cacctgagcc ttcaccgcat
     1141 gggggaccga aacccagtga agggttccct tgacccggcg cctgtcgggg gcctctccgc
     1201 cgcggctctg cggatcatag gtgcagcgaa gttctgtgat ctctccatcc ccgttcctga
     1261 cgacttcggt gcatgtgaca aggtaggcat gcttcagccg cacttccgtc ccaggagaaa
     1321 gacggaaaaa tttc
//
LOCUS       DAOTHD010000732         1334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_119259_length_1334_cov_2.526192, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000732 DAOTHD010000000
VERSION     DAOTHD010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119259_length_1334_cov_2.526192"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="P5219_10905"
     CDS             complement(<1..462)
                     /locus_tag="P5219_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HRX27318.1"
                     /translation="MAVVKRTSSNVLLDTALKNFYAAAEEMGLEEGLIDILSHSERRT
                     CVSIPVEMDDGTVRVFDGYRVLHSSAIGPGKGGVRFHPEVNLDECEALGMMMTWKCSL
                     AGIPYGGGKGGVSCEPLELSKKEKERIARTWAARMAPVIGAWTDVPAPDVNT"
     gene            complement(963..>1334)
                     /locus_tag="P5219_10910"
     CDS             complement(963..>1334)
                     /locus_tag="P5219_10910"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HRX27319.1"
                     /translation="GQGILAVRIDPDGKDALTRYRLLAGGNDASLVEIDLVTGRSHQA
                     RVHMASIGYPVLGDIRYGSEETNGKWRKRGIRRPLLHSYRIFFGELGGEIEDLSGMRI
                     TAPPAGDMESLCVSRGWRVPG"
BASE COUNT          294 a          412 c          318 g          310 t
ORIGIN      
        1 ggtgttgacg tccggtgcgg gaacgtcggt ccatgcgccg atcacgggag ccatgcgggc
       61 agcccaagtc ctggcgatcc gttctttttc cttcttggaa agctcgagag gttcgcagga
      121 aacgccgccc ttgcctccgc cgtagggaat tccggcgagg gagcatttcc aggtcatcat
      181 cataccaaga gcttcgcatt cgtcgaggtt cacttcaggg tggaatcgga ctccgccctt
      241 gcccggcccg atggcggatg aatggagaac cctgtacccg tcaaaaaccc ggaccgttcc
      301 gtcatccatt tccacgggga tggaaacgca tgtccggcgc tcggaatggc tgaggatgtc
      361 gatgagtccc tcttcgagcc ccatctcctc agctgctgca tagaaattct tgagtgctgt
      421 gtcaaggaga acgtttgagg aagtacgctt tactacggcc attaaacaca cctcctgtat
      481 ttttacggct tccgccgttt gagactgttc atatcataac atttttttcc caaaagcacc
      541 aagaaaagag aagaaatagg tcaaaatagt tccaaatacc taaaacaaaa tattacaatt
      601 cattcgccgc aatcggagca atccgcacaa aaagagccgt tcccggagtc aacactcgtg
      661 accatgaggt aggcagacca caaaaagccc cgctgcacca cttctatcat ttcattcctg
      721 aacttcagaa atgaaggaac ccagagagtc actccatcct gctcgatttt gcggtactcg
      781 ccctccgacg gaggggcgcc gtcttcaacc ctgaagactc tccttgctcc tcacccaagg
      841 ccgagagtcc gctggatgac aaatgcctcc ccctttttcc ggagcttttc cataacgctt
      901 cgttccgcga ctagtttcag ttccattttc atcctcctct cctcacctgc agctcttctt
      961 ttttatcccg gaacccgcca tccccgggag acacagaggg attccatgtc tccggcagga
     1021 ggagcggtaa tcctcatgcc ggacaaatcc tcgatttctc cccccagttc cccgaaaaaa
     1081 atcctgtagc tgtgaagcag cgggcgccgg attccccgtt ttctccattt cccgttggtt
     1141 tcctcgctcc cgtaccgtat gtcgcccagg acagggtatc cgatggatgc catgtggacc
     1201 ctcgcctggt gtgatcgtcc cgtcacaaga tcgatctcaa ccagagacgc gtcgtttccc
     1261 cccgcgagaa gcctgtacct ggtcagggca tccttgccgt ccggatcgat ccggacggca
     1321 aggatgccct gacc
//
LOCUS       DAOTHD010000733         1332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_119558_length_1332_cov_2.039154, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000733 DAOTHD010000000
VERSION     DAOTHD010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119558_length_1332_cov_2.039154"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..1208)
                     /locus_tag="P5219_10915"
     CDS             complement(150..1208)
                     /locus_tag="P5219_10915"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRX27320.1"
                     /translation="MRMIITKRAPLPGWVQVLVPVGAILVTLLLSAVPILFAGGDLWL
                     SYTSLFKGALGTRYNFLETCVKAAPLTFTGLAVAFAFRAKFWNIGAEGQLLAGAIAAT
                     WVGISAPALPKVAVLILVCAAGFLAGGLWATVPALLKTKYRVDDVVTTLLLNYVMWHI
                     MGYLLFGPLQMPNSSWPRSPAILEIARFPVLLARSRFHLGIVLAVAAVLIVWFINSKT
                     VFGYQARAVGVNPRAAAFGGINVNSVIIRTALLSGGLAGMAGVGEVAAIHFHLLMDIS
                     PGFGYSGIVIAMLGRLHPLGTALAAFFFSVIIVGAQSMSRLTGVPTYIAEVIQGMALI
                     VMLIALLLTEYRIKAVKD"
BASE COUNT          258 a          385 c          476 g          213 t
ORIGIN      
        1 catgagcatg atcccctcga tgccgaggtt gaggacgccg gcccgctcgc tgaggatttc
       61 ccccagggtg ccgaagatga tgggggtggc catgcgcatc atggcggcga ggagcccggt
      121 gatgaatgta atggtgaata cctgttccat cagtctttca ccgcctttat gcggtattcg
      181 gtgaggagga gggcgatgag catcacgata agggccatgc cctggatcac ctcggcgatg
      241 taggtcggga ccccggtgag gcggctcatg gactgggccc ccacgatgat gacgctgaag
      301 aagaaggcgg ccagggccgt ccccagggga tggagcctgc ccagcatggc gatgacgatt
      361 cccgaatacc cgaagccggg ggagatgtcc atgaggagat ggaagtggat ggccgccacc
      421 tcgccgacgc ccgccattcc ggcgagcccg ccggagagga gggcggtcct gatgatcacg
      481 gagttgacgt tgatgccgcc gaaggccgcc gcccggggat tgactcccac ggccctcgcc
      541 tggtatccga agacggtctt cgagttgatg aaccagacga tgaggacggc cgcgacggcg
      601 agcacgatgc ccaggtggaa ccgtgacctg gccagcagca cggggaaacg ggcgatctca
      661 agtatcgcgg gagacctcgg ccagctcgag ttgggcatct ggagggggcc gaagagcagg
      721 tagcccataa tgtgccacat gacgtagttc aggaggaggg tggtgaccac gtcgtccacc
      781 ctgtattttg tcttcagcag ggcggggacg gtggcccaga gtcctccggc gaggaagccc
      841 gcggcgcaga cgaggatgag cacggcaacc ttgggaaggg cgggggcgct gatgcccacc
      901 caggtggcgg cgatggcccc cgccaggagc tgcccttccg cgccgatgtt ccagaacttg
      961 gcccggaagg cgaaggccac ggccagcccg gtgaatgtga ggggagcggc cttcacgcag
     1021 gtttcgagga agttgtaccg ggtgcccagg gctcccttga agagggacgt gtaggagagc
     1081 cagaggtctc cgccggcgaa cagtatcggg acggcggaaa gcaaaagggt caccaggatg
     1141 gctcccacgg ggacgagcac ctggacccat cccggaaggg gggcccgctt cgtgatgatc
     1201 atgcgcatgg ttcgttcacc ccgctcatcc agagtccgat ctgctccctg gacgccgagc
     1261 cgccgtcggc gatgcccatg atgcggcctt cgtacatgac ggcgatccgg tcgctgagct
     1321 ggaatatttc gt
//
LOCUS       DAOTHD010000734         1331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_119642_length_1331_cov_3.237461, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000734 DAOTHD010000000
VERSION     DAOTHD010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119642_length_1331_cov_3.237461"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="P5219_10920"
     CDS             <1..533
                     /locus_tag="P5219_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012799628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_except=(pos:270..272,aa:Sec)
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HRX27321.1"
                     /translation="SGVMDLTADGYCTAHGGYDATYADQDADRVLPVDVLRDMEKEGV
                     FKKLHNKFYTTVGNGTAVASAKKFGAEIARRLIADGVTAVILTSTUGTCTRCGATMVK
                     EIERAGLPVVHVCTIVPISLTVGANRIVPAIAIPHPLGDPTRTMEEEKEIRRHLVQKS
                     LKALQTEISDQTVFAD"
     gene            612..>1331
                     /locus_tag="P5219_10925"
     CDS             612..>1331
                     /locus_tag="P5219_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HRX27322.1"
                     /translation="MATAGIKGYAYCLNHAPETGFHYGNTPFIERETKGETEFLRELP
                     SFMQKYEEARDYAPNQAYIGGIGLEALEKSPQPWYVNRMTGSNRYGAYGEIMPEDEFL
                     GLMDICDVFDIIWLETSFAASVREKLAASPVMNDKLLARLEGGHSADEIAAETAHKKA
                     LPLYFNGAVVGCARNGHEVDDCLFAYVLLENLACKAGGVLSLLHLLKNTGMAPEEVDF
                     VIECSEEGAGDMNQRAGGNFAK"
BASE COUNT          300 a          423 c          382 g          226 t
ORIGIN      
        1 tctccggcgt gatggacctc accgccgacg gctactgcac ggcccacggc ggctatgacg
       61 ccacctacgc cgaccaggac gccgacaggg tcctccccgt ggacgtgctt cgcgacatgg
      121 agaaggaagg cgtcttcaag aagctccaca acaagttcta taccaccgtg ggcaacggaa
      181 cggccgtggc cagcgccaag aagttcggcg ccgagatcgc ccggaggctc atcgccgacg
      241 gagtgaccgc cgtcatcctc acttccacct gaggaacctg cactcgttgc ggcgcaacga
      301 tggtcaagga aatcgagcgt gcaggccttc ccgtagttca cgtatgcacc atagtcccca
      361 tctccctcac cgtgggagcc aacaggatcg ttcccgcaat tgccattccc catcctctcg
      421 gtgatcccac gaggaccatg gaagaagaga aggagatccg gcgccatctg gtccagaaat
      481 ccctgaaggc gcttcagacc gaaatttccg atcagacggt gttcgccgac tgacggtttt
      541 tcatgcgggg gcccggagat tttccgggcc ccacagccca agagagaaca gcacaaggag
      601 gttggacccc catggccacc gcaggaatca aaggatatgc ctactgcctg aaccacgccc
      661 cggagacggg attccactac gggaacacgc cgttcatcga acgagagacg aagggtgaga
      721 cagagttcct ccgggaactg ccgtccttta tgcagaaata cgaagaagcc cgggactatg
      781 cccccaacca ggcctacatc ggcggcatcg gcctcgaggc cctggaaaaa tccccgcagc
      841 cctggtatgt caacaggatg accggttcaa accgctacgg ggcttacggt gagatcatgc
      901 ccgaggacga gttcctcggc cttatggata tatgcgacgt cttcgacatc atctggctgg
      961 agacctcttt tgccgcctcc gtcagggaaa agctcgccgc gagtccggtg atgaacgata
     1021 agctgctcgc ccggctcgaa gggggccatt cagccgacga gatcgccgcc gagacggccc
     1081 acaaaaaagc ccttcccctg tatttcaacg gagcggtcgt gggctgcgcc cggaacggac
     1141 acgaggtgga cgactgcctc ttcgcctatg tcctcctcga aaaccttgcc tgcaaggccg
     1201 gaggagtgct ctccctcctt cacctgctga agaacacggg catggcgccc gaagaagttg
     1261 attttgtcat tgaatgctcc gaggaaggcg caggagacat gaaccagagg gccggcggca
     1321 acttcgccaa g
//
LOCUS       DAOTHD010000735         1331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_119647_length_1331_cov_3.075235, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000735 DAOTHD010000000
VERSION     DAOTHD010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119647_length_1331_cov_3.075235"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="P5219_10930"
     CDS             complement(<1..227)
                     /locus_tag="P5219_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27323.1"
                     /translation="MEYGSLLLLFDEENPCWTEKLVQLADEDPALLADIVRDGLAERK
                     GEICVLTGEGREAFRKEAAESYLSAEPGIPGL"
     gene            complement(386..>1331)
                     /locus_tag="P5219_10935"
     CDS             complement(386..>1331)
                     /locus_tag="P5219_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HRX27324.1"
                     /translation="LAIFATIAGLATSLGLGTLQINSGLKYLFDMPQNVTLQLVIIAA
                     LAFIYTGTAVTGIERGIMFISNLNLFLACLLSMALFFIGPTMAIVESLMTGLGDYLSN
                     VVNQSFHMAPYGGEYKGWLGGWTLYYWAWWIAWAPFVGSFIARISRGRTIREFVSGVL
                     VVPALGSFTWFAIFGTSGLNLEVTGVAKIAEKVAADISVGVFEVYKYYPLGGVMSVIM
                     VALISTFFITSANSGTFVLSMYSTQGDMNPPKVRMGIWGILMAALAFVLLMSGGLQSL
                     QIASIAAAAPFAVIMVVACWCLWKALVKDEATFAELAD"
BASE COUNT          294 a          392 c          414 g          231 t
ORIGIN      
        1 aggccgggga ttcccggttc agccgaaagg tagctttcag cggcctcctt ccggaacgcc
       61 tccctcccct cgccggtcag gacgcaaatc tctcccttcc tctccgccag accgtcccgg
      121 acgatgtccg ccagaagcgc cggatcctca tcggcaagct gcacaagctt ttcggtccag
      181 caggggtttt cctcgtcaaa caacagcaga aggcttccgt attccatgct cttttcctcc
      241 tctcacggtg cgcggcactc cctgcagacg acgcccccgg gcgaaaaagc gccgggggcg
      301 tcgtctatcg ggaaaatcgc aagcttcaat tcactttcgg gcaccctcct gaaggatagc
      361 cgtaaatgac ggtttcttcc aggggtcagt ccgcaagttc ggcaaaggtg gcttcgtcct
      421 tcacgagggc cttccacagg caccagcagg ccaccaccat gataaccgcg aagggggcgg
      481 cggcagcgat agaggcaatc tgcagcgact gcagcccgcc gctcataagg agcacaaagg
      541 caagagccgc catcaggatg ccccagatgc ccatgcgcac cttcggaggg ttcatgtcgc
      601 cctgggtgga atacatggag aggacgaagg ttcccgagtt ggccgacgtg atgaagaagg
      661 tggagatcag cgccaccatg atgacggaca tgacgccgcc cagggggtag tatttgtaga
      721 cttcgaaaac gcccacggaa atgtccgccg ccactttctc cgctatttta gccacgccgg
      781 tgacctcgag gttcagcccg gaggtgccga agatggcgaa ccacgtaaag cttcccaggg
      841 ccggtacaac cagtacgccg gagacgaatt cccggatggt gcgccccctg gaaatgcggg
      901 cgatgaacga accgacaaag ggagcccagg cgatccacca tgcccagtag taaagggtcc
      961 agcctccgag ccatcccttg tactcgccgc cgtagggagc catgtggaag ctctggttca
     1021 ccacgttgga aagatagtcg ccaaggcccg tcatcaggga ttcgacgatg gccatggtgg
     1081 ggccgatgaa gaagagggcc atgctcagca ggcaggccag gaaaagattg aggttcgata
     1141 tgaacatgat gccccgctcg atgccggtga ccgcggtccc ggtatagatg aaggcgagag
     1201 cggcgatgat gacaagctgg agggtgacgt tctggggcat gtcgaagagg tacttcaggc
     1261 cgctgttgat ctggagagtt ccaaggccga gggacgtggc gaggccggcg atggtggcga
     1321 agatggcgag a
//
LOCUS       DAOTHD010000736         1331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_119683_length_1331_cov_2.466301, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000736 DAOTHD010000000
VERSION     DAOTHD010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119683_length_1331_cov_2.466301"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="P5219_10940"
     CDS             complement(<1..898)
                     /locus_tag="P5219_10940"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRX27325.1"
                     /translation="MKKRHLLVLLVLAVSLACAGALSAAENYPAKPVVLVYHSQAGSG
                     GDIFLRNMGKAIEKIIGQPIIVENRTGGGGSNAWMYSKNAKPDGYTLLGISSSVIAGP
                     LQTKMAVSYADFKPVAQVFFDPTVIFVPAKSPFKTFDDVIKDARANPGKQNWGAGNPG
                     SAETMCIEKVAQMADMKITVVPFEGGADVMVEIIAGRIDAAIGEYAEIASQVEAGNIR
                     IIACLNTERMEEAPDVPTLKESGVDFVFEKIRGIVAPKDVPDSVVKVWTDSLAKIYDD
                     PEFKKYYRENMLVPRFVPAEKMQ"
     gene            complement(1029..>1331)
                     /locus_tag="P5219_10945"
     CDS             complement(1029..>1331)
                     /locus_tag="P5219_10945"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRX27326.1"
                     /translation="AEKEGISVEGPFPSDTLFIRAFEGKYDGVVTMYHDQGQIAIKLR
                     GFHHCVTVSAGLPHPITTPAHGTAYDIAGKGICTTSAFEDAYALAARMALGDITER"
BASE COUNT          232 a          405 c          382 g          312 t
ORIGIN      
        1 tctgcatttt ctcggcggga acaaatctcg gaacgagcat gttctcccgg tagtatttct
       61 tgaattcggg atcgtcgtaa atcttggcaa gagagtccgt ccacaccttg accacggaat
      121 ccggtacatc cttgggggca acaatgccgc ggatcttctc gaagacgaag tccacgccgc
      181 tctccttgag ggtggggacg tcgggagctt cctccatcct ctcggtgttc aggcaggcga
      241 tgatgcggat gttccccgcc tccacctggc tggcgatctc ggcatattcg ccgatagccg
      301 catcgatccg tccggcgatg atctccacca tgacgtcggc gccgccctcg aaaggcacca
      361 cggtgatctt catgtcggcc atctgggcga ccttctcgat gcacatggtc tcggcgctgc
      421 cggggtttcc ggcgccccag ttctgctttc cgggattcgc cctggcatcc ttgatcacgt
      481 cgtcaaaggt cttgaacgga ctctttgcgg ggacgaaaat aaccgtggga tcgaagaaga
      541 cctgggctac aggcttgaag tcggcgtagg aaaccgccat ctttgtctgg agcggaccgg
      601 cgatgacgga ggacgaaatg ccgagcaggg tatagccgtc gggcttcgcg ttcttggaat
      661 acatccaggc gttggagccg ccgccgcccg tcctgttttc cacgatgatg ggctggccga
      721 tgatcttctc gatggccttt cccatgttcc tcaggaagat gtcccctccg gaacctgcct
      781 gggaatggta cacgagcacg accggcttcg cgggatagtt ctccgcggcg gaaagggcgc
      841 ccgcgcacgc cagagacacg gccagaacca gaagtaccag aagatgtctt tttttcattt
      901 tcgtgtaacc ctccttattt ttgttattcc tgcttctctc cacgctgctg ccgcaaggtt
      961 tctttcttca tccatccctc ccttcgccat ccggaagaac cggccgccgt tcctgccggc
     1021 tctttctctt atcgctccgt gatatctccc agggccattc tggcggcgag ggcataggcg
     1081 tcctcaaagg ctgacgtggt gcatatcccc tttcccgcga tgtcgtaggc cgttccgtgg
     1141 gcgggcgtgg tgatcgggtg gggcaacccc gccgagacgg tgacgcagtg atggaatccc
     1201 ctgagcttga tggcgatctg tccctggtcg tggtacatgg tgaccacgcc gtcgtatttt
     1261 ccttcaaagg cccggatgaa cagggtgtcc gacggaaagg gcccctccac ggaaatgccc
     1321 tctttttccg c
//
LOCUS       DAOTHD010000737         1331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_119689_length_1331_cov_2.382445, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000737 DAOTHD010000000
VERSION     DAOTHD010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119689_length_1331_cov_2.382445"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..807)
                     /locus_tag="P5219_10950"
     CDS             complement(7..807)
                     /locus_tag="P5219_10950"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HRX27327.1"
                     /translation="MKKRSIFTTGLLAAAVLVLLGGALAWGAPGRWRSGEPSFRAELF
                     RAVNPLDFTEEQWAKADGILEKLREQKWTAARVLFGLRGEARMLFSGAEPFDGKKAGE
                     ALAAARPGLLEVAEGALRAAADLYGMLDEKQRAKVENVLSLLEKRAEQHIADFPGKHP
                     HFRRDFLDLTKEQREKAENLFMASVPAMQERMGRLLAVLKEGRAALRDGKMNDEMIGK
                     TAEKAVDILSEGALAMAGTYGEFRGMLTPEQLEKADRMWSRHGRRPRK"
     gene            1042..>1331
                     /locus_tag="P5219_10955"
     CDS             1042..>1331
                     /locus_tag="P5219_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX27328.1"
                     /translation="MHTLLLLDDDRELCDLLTDYLKPEGFEILTAHDGNTGLAAALNF
                     PSLDLILLDVMLPGKNGFDVLRELRAHSGVPVLMLTAKGDEVDRIVGLELGA"
BASE COUNT          234 a          464 c          362 g          271 t
ORIGIN      
        1 cgtccgttat ttcctcgggc gccggccgtg gcggctccac atcctgtcgg ccttttcaag
       61 ctgttcgggg gtcagcatcc cgcggaattc gccgtaggtt cccgccatgg cgagggcgcc
      121 ttcggaaagg atgtccacgg ccttttcggc ggtcttcccg atcatctcgt cattcatttt
      181 tccatcccgg agggcggccc ggccttcctt cagcacggca agcagcctgc ccatccgctc
      241 ctgcatggcg gggacggaag ccatgaagag attttcggcc ttttcccgct gctcttttgt
      301 caggtccagg aaatccctcc ggaagtgagg atgtttcccg ggaaaatcgg ctatgtgctg
      361 ctcagcccgc ttctccagga gggaaaggac attttccacc ttggcgcgct gtttttcgtc
      421 cagcataccg tacaggtccg cagcggcccg gagggctccc tccgccacct ccaacagtcc
      481 ggggcgcgca gccgcaaggg cttccccggc cttttttccg tcgaagggct cagcaccgga
      541 aaacagcata cgggcctccc cccgaagacc gaacagaacc ctggccgcag tccatttctg
      601 ctcccgcagc ttctccagta tgccgtccgc ctttgcccac tgctcttcag tgaaatccag
      661 cgggttgacc gcccggaaca gttccgccct gaaggacggc tctccgctcc tccaccgccc
      721 cggagccccc caggccagcg ctcccccgag aagaaccagg accgccgctg ccaacagccc
      781 cgtggtaaat atcgatcgtt ttttcatgtg ttctgcctcc ctccgcaaga tgccggggta
      841 ttccccggta cggcaggatc atacaccgga gagcatgaag gtgcccttca gccaatccgc
      901 aggaacatca agaaaagtaa acctcttctc ccccttcttt accaaccttt accccccgtc
      961 ccttcccgct gtcgtataat ggcttcagcg ccgggaaggt tattgtcccg gccgattcat
     1021 gcacgagagg aggcgatggc cgtgcatacc cttcttctgc tcgacgacga cagggaactc
     1081 tgcgacctgc tgacggacta cctgaagccc gagggatttg aaatcctcac cgcccacgac
     1141 ggcaacacgg gactggcggc cgccctgaac ttcccgtccc tggacctcat tctgctcgac
     1201 gtgatgctcc ccggaaagaa cggcttcgac gtcctccggg agctgcgggc ccattccggg
     1261 gttcccgtcc tcatgctcac cgccaagggg gacgaagtgg acaggatcgt gggcctcgaa
     1321 ctgggggcgg a
//
LOCUS       DAOTHD010000738         1331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_119690_length_1331_cov_2.368339, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000738 DAOTHD010000000
VERSION     DAOTHD010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119690_length_1331_cov_2.368339"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..>1331
                     /locus_tag="P5219_10960"
     CDS             159..>1331
                     /locus_tag="P5219_10960"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRX27329.1"
                     /translation="MSVRLEEKIAHRFSGDPSQPVLWWKGVWEDAAETERKTALCAET
                     LRRGGFQPGSRLAAFLPNSPLVLHLSMAAWRLGGAIVPLNPRGGTEWILKILHHVDPF
                     GVVLGDGMEALGEAVRAEGIPAVEASPDGTVPPLTGRRDRVEQDPDVAVVFATSGTTG
                     APKAVPLTHGNLYDNTKGVHETVEGFDRGRVLMNVLPNFHSFGYTVCGVLPLVWGLPE
                     ALLPGFLPLKNFFEGLRESKTGTLIAVPTMLPFLLGVASKGETLPPELRYILTGGGKL
                     DPALEKRFRDQLGVITFEGYGLTECSPVVSSNPSDALRKTGTVGPALPGYSVEVRDSE
                     GKALPRGEEGVLWLKGPSVARGYFRDPVLTAERFKDGWLNTDDMARVDEDGYITILD"
BASE COUNT          240 a          429 c          429 g          233 t
ORIGIN      
        1 gcagtcaaga tacagggtct tcatcagata cctccggttt tcgcgtccgg ccgggggggg
       61 ccgtttttcc ccattctatc atccccgggg ccgaaaaaag gggggagaat gtgctatcct
      121 tatccggaaa gttcctgaga aaaaaggagg atccgtccat gtccgttcgc ctcgaggaga
      181 agattgcgca caggttttcc ggggatccgt cccagccggt gctctggtgg aaaggtgtct
      241 gggaggatgc cgctgagacg gagagaaaga ccgccctctg cgcggaaacc ctccgccgcg
      301 gaggattcca gcccggcagc cgcctcgcgg cgtttctgcc caacagtccg ctggtgcttc
      361 atctgtccat ggcggcctgg cggctcggcg gcgccatcgt tccgctgaat cccagaggag
      421 gaacggaatg gatcctgaaa attctccacc atgtggaccc gttcggcgtg gtcctcggtg
      481 atgggatgga agccctgggg gaggcggtcc gggcagaggg gatacccgcc gtggaagcgt
      541 cccccgacgg aacggtaccg ccgctcaccg gacgacggga ccgggtggaa caggatcccg
      601 atgtggcggt ggtcttcgcc acctccggca ctacgggtgc gccgaaggcg gttcccctga
      661 cccacggcaa tctctacgac aacacgaagg gcgtccacga aaccgtggag ggctttgaca
      721 gaggccgggt cctcatgaac gtgctgccca acttccattc cttcggctac acggtctgcg
      781 gtgtcctgcc cctcgtctgg ggactccccg aggcccttct ccccggattc ctccccctga
      841 agaatttctt cgagggactg agagaatcga agacgggcac cctcatcgcc gttcccacca
      901 tgctcccctt ccttctcggc gtggcctcca agggggaaac cctgcccccg gagctgcgct
      961 acatcctcac gggcgggggc aagcttgatc cggccctgga aaagcgcttc cgggaccagc
     1021 tcggcgtcat caccttcgag gggtacggcc tgacggaatg ttcccccgtc gtatcgagca
     1081 accccagcga cgccctccgc aagaccggta ccgtggggcc cgccctgccg ggctactcgg
     1141 tggaggtccg ggacagcgag ggaaaggccc tcccgagagg ggaagaagga gtcctctggc
     1201 tgaaagggcc ctccgtggcc cgggggtatt tccgggatcc cgtcctcacg gcggagcgct
     1261 tcaaagacgg ctggctgaac accgacgaca tggcgcgggt ggacgaggac ggctacatca
     1321 ccattctcga c
//
LOCUS       DAOTHD010000739         1330 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_119812_length_1330_cov_2.555294, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000739 DAOTHD010000000
VERSION     DAOTHD010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119812_length_1330_cov_2.555294"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..1272
                     /locus_tag="P5219_10965"
     CDS             115..1272
                     /locus_tag="P5219_10965"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRX27330.1"
                     /translation="MKFAERYAGLQPSGMLKIFQAAAADPDMVNLGEGEPDFDTEHDI
                     IDAAAKAAKEGYTHYGPIMGFEDVRRSVCGYWDRRYGLKSSPDEVMIMAGGVQSVHLA
                     LQALLDPGDEVITAEPCFSPYFEQIYQHRGTAVHLPTTEEDGFVPTAEALERAVTPKS
                     KVLMICSPSNPTGRVMTRRQMEDIAAVAERHDLTVLSDEIYDSLVFKGRHVPFATIPG
                     MKERTLTMGGLSKSHCMTGWRIGYAIGPAPLVRLMALIGANQTYGVNVPTQMASKYAL
                     DNHDSKLEERAGIFRQRLGYVAERLNAMPGIRCSEPEGAFYLFPDIRGTGLSSEGFAW
                     GLLEKGKVATLPGSAFGACGEGYVRLACTRSLEVLAEGMDRMEAFVKSLKG"
BASE COUNT          263 a          424 c          437 g          206 t
ORIGIN      
        1 catgctgaag gaatacgagt agagaggaca ttccccgccc gcaccctccc ggggtgcggg
       61 catttttctt cccccggcgg ggaaatacga ctcagagaac aaggagaggg tagcatgaag
      121 ttcgcagaac ggtacgcggg actgcagccc tccggcatgc tgaagatctt ccaggccgcc
      181 gcggccgacc ccgacatggt caacctggga gagggagagc cggatttcga cacggagcac
      241 gacatcatcg acgccgccgc gaaggcggcg aaggaaggat acacccacta cggccccatc
      301 atggggttcg aggacgtccg ccggtctgtg tgcggctact gggacaggcg gtacggcctg
      361 aaatcatccc ccgacgaggt gatgatcatg gcgggaggag tccagtcggt ccacctggcc
      421 ctccaggccc tactcgaccc gggcgatgag gtgatcacgg cggagccgtg cttttctccc
      481 tatttcgagc agatttacca gcaccgggga acggcggtcc accttccgac gacggaggag
      541 gacggcttcg tccccacggc ggaggccctg gaacgggcgg tcacgccgaa gtcgaaggtg
      601 ctcatgatct gctcccccag caaccccacc ggaagggtca tgacccggcg gcaaatggag
      661 gacatcgccg ccgtggctga acggcacgac ctcacggtgc tctccgacga gatctacgac
      721 tccctggttt tcaagggacg gcacgtgcct ttcgccacca tccccggcat gaaggagagg
      781 accctgacca tgggggggct ctccaagagc cactgcatga cgggatggcg catcggctac
      841 gccatcggcc cggcgcccct ggtccggctc atggccctta tcggcgcgaa ccagacctac
      901 ggagtcaacg tcccgaccca gatggcctcg aagtacgccc tggacaacca tgacagcaag
      961 ctcgaggagc gagccgggat cttccggcag aggctcggct acgtggcgga acggctcaac
     1021 gccatgcccg gcatccggtg ctccgagccc gaaggggcct tctacctctt cccggacatc
     1081 cggggcacgg ggctttcgag cgagggattc gcctgggggc tcctggagaa gggaaaggtg
     1141 gccacccttc cgggatcggc cttcggcgca tgcggcgagg gctacgtccg tctggcctgc
     1201 acccggtccc tcgaggtgct tgccgaagga atggaccgca tggaggcctt cgtgaagtct
     1261 ctgaagggat agaagcgttt tttcaaaaaa agaacaggag cggcccctcc ccggcatggg
     1321 agagcgggcc
//
LOCUS       DAOTHD010000740         1328 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_120057_length_1328_cov_3.698350, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000740 DAOTHD010000000
VERSION     DAOTHD010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120057_length_1328_cov_3.698350"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..>1328)
                     /locus_tag="P5219_10970"
     CDS             complement(172..>1328)
                     /locus_tag="P5219_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27331.1"
                     /translation="QACVLPAPAAKEEWVTYGNARFGYSVEYPDIFTVLEEPDNGDGI
                     WLKTEGDRYALTISGGFNVLGEDGETRLQARIREAAHIVPGSDGAGPGWYRVIYSDDG
                     GRKGKERRFHEYGILDGENWASFILVYPLEEVARFSAAAARMEKSLALPSSGEGEDGG
                     AFRPDAYTLRDGRVFLDEKALDCEVHEIPAGLDNGITHWAVTGKEFSEDVTQEETGVW
                     FFGPEGMVVTFIPLDSGNEYRDLLWSPAGDRLVLARGSGTRPDLFFDVFGEGMEKIAE
                     FSGLRGEAAWLDDGMRFVFTRIDDTREGAVFPGLSYGLKVSAVLYDSAVEETIPLKEA
                     DETRNYTFLSVSPDGMDIRIVETSVLSPGHWADTEKWKEREITVPVPAAG"
BASE COUNT          261 a          441 c          347 g          279 t
ORIGIN      
        1 tcatgccggc ataatactgt ccgggccttt cgccgttccc tcccggcacc cgtttcagaa
       61 accgcaggga ggcacacaac cttgtcctgc ttccctgaaa caataccatg aaggagccat
      121 tcgcggccaa tattcttcag acaatttcat actatcaggc agaattcacc cttatcccgc
      181 cgccggcacg ggaacggtta tctcccgttc cttccatttt tccgtgtctg cccagtggcc
      241 cggagacagg acggacgttt cgacaatccg gatgtccatt ccgtcggggg agaccgaaag
      301 gaaagtgtag ttccttgtct catcggcttc cttcagggga atcgtctcct ccaccgctga
      361 atcgtagagg acggcggaga ctttcaggcc gtaggaaagg ccggggaata ccgctccctc
      421 ccgggtatcg tcgatccggg tgaaaacaaa gcgcatgccg tcgtcaagcc aggcggcttc
      481 tccccgcaaa ccgctgaatt ccgctatttt ctccattcct tccccgaaca cgtcgaagaa
      541 cagatcgggc cgtgttccgc ttcccctggc gaggacaagg cgatccccgg cggggctcca
      601 gaggaggtcc cggtactcgt tcccgctgtc caggggaatg aaggtcacaa ccattccttc
      661 gggcccgaag aaccagacgc ccgtttcctc ctgtgtcacg tcctccgaga attccttccc
      721 tgtcacggcc cagtgggtga ttccgttatc cagcccggcc ggaatttcat gcacttcgca
      781 gtcgagagct ttttcatcaa gaaacacccg gccgtcccgg agagtatagg catccggccg
      841 gaatgctccg ccgtcttccc cctcgccgga agacggaagg gcaagacttt tctccattcg
      901 ggcggccgcg gcggaaaagc gggccacctc ctcaagggga tagacgagga taaaggatgc
      961 ccagttctcc ccgtcgagaa taccgtactc gtggaagcgg cgctcctttc ctttcctgcc
     1021 cccgtcgtcg ctgtagatga ccctgtacca tcccggaccc gcgccatctg atccgggcac
     1081 gatatgagcc gcctcccgaa tccgggcctg cagcctggtt tccccgtcct ctcccagcac
     1141 gttgaagccg ccggagatgg tcagggcata cctgtcccct tccgttttca gccatatgcc
     1201 gtctccgttg tccggttcct ccaggacagt gaagatatca ggatattcca cagagtaacc
     1261 gaaacgggca ttcccatagg tgacccactc ttccttcgcc gccggagcgg ggagaacaca
     1321 tgcctgga
//
LOCUS       DAOTHD010000741         1324 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_120697_length_1324_cov_2.085106, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000741 DAOTHD010000000
VERSION     DAOTHD010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120697_length_1324_cov_2.085106"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="P5219_10975"
     CDS             <1..662
                     /locus_tag="P5219_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRX27332.1"
                     /translation="FSGMEDYLEKAARFLARAADQGNRLVCLPPMKTEGCSGAAEKLS
                     GLLAGSDVVAVLNGPAGEDRETVVFSAGGVIGRFSGDVLEPLDTPAGRIGAVWGDEGW
                     NPEPVRCLMLGGAEMVVWYGDGKRPREISVKVARTRAAENRIFLLYYHGGEDALVAGP
                     SGAVLAETLDEGDQAVSAMIRRSESRGKTVVPGTNIVSGRLPSAYGGLVGEKGANGSH
                     D"
     gene            655..1125
                     /gene="tadA"
                     /locus_tag="P5219_10980"
     CDS             655..1125
                     /gene="tadA"
                     /locus_tag="P5219_10980"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HRX27333.1"
                     /translation="MIDRDERFMVLALEEARAAALRGDVPIAALVVRDGEVVSAGSNR
                     KERDPTAHAEIIALRRAAEAAESWNLRGCTLYVTVEPCPMCAGALVLARVDRLVYGCA
                     DPRAGACGTLYDIVRDSRLNHRCAVRRGVLERECARLLTDYFLERRKSRFPSGL"
     gene            1141..>1324
                     /locus_tag="P5219_10985"
     CDS             1141..>1324
                     /locus_tag="P5219_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRX27334.1"
                     /translation="MKFLSYGHQYIDEEDIRAVADVLRGDWLTQGPSVDAFEKAFAEY
                     VGTRHAVAFANGTAALH"
BASE COUNT          244 a          355 c          473 g          252 t
ORIGIN      
        1 gtttctccgg catggaggac tatctggaaa aagcggcccg atttcttgcc cgtgcggctg
       61 accaggggaa caggctggtc tgtctcccgc ccatgaagac tgaagggtgt tccggagctg
      121 cggaaaagct gtcgggtctc ctggcgggaa gcgatgttgt cgccgtcctg aacggcccgg
      181 caggtgagga cagggaaacc gtcgtgtttt ccgcaggcgg tgttatcggc cggttctccg
      241 gagatgtgct cgagcccctg gacactcctg cgggaaggat cggggcggta tggggggacg
      301 aaggctggaa cccggagccg gtgcgctgcc tcatgctcgg aggagcggaa atggtggtgt
      361 ggtacggtga cggaaagcgt ccccgggaga tatccgtaaa agttgcccgt accagggctg
      421 cggagaacag gattttcctg ctgtattacc acggcggtga agatgccctt gttgcggggc
      481 cgtccggcgc ggtgctggcg gaaactcttg atgaaggaga ccaggctgta tcggccatga
      541 tccggagatc ggaaagccgc ggaaagactg tggtgccggg gacgaacatc gtgtccggca
      601 ggctgccctc cgcctatggc ggccttgtcg gcgaaaaagg cgctaacggt tctcatgatt
      661 gaccgggatg aacggttcat ggtcctggcg ctggaggagg cgcgggcagc ggccctgcgg
      721 ggagatgtcc ccatcgccgc cctggtggtg cgggacggag aggttgtttc cgcgggaagc
      781 aaccggaagg aaagagaccc gaccgcccac gctgaaatta tcgcccttcg gagagcggct
      841 gaggcagcgg agtcctggaa cctgcggggg tgtactcttt acgtcaccgt tgaaccttgt
      901 cccatgtgtg ccggtgcgct tgttctggca cgggtggacc gcctcgttta cggatgcgcc
      961 gatcccaggg ccggggcgtg cggcaccctg tacgacatcg tcagggattc gaggctcaat
     1021 caccggtgcg ctgtacggag gggggttctc gagagggagt gcgcccggct cctcaccgac
     1081 tattttcttg aacgcagaaa gagccgcttc ccgagcggcc tgtaagaaag ggtgaaggaa
     1141 atgaaattcc tgtcctatgg tcaccagtat atcgatgaag aggatatccg ggccgtcgcg
     1201 gacgtgctca ggggtgactg gcttacccag ggaccgtcgg tggacgcttt tgagaaagcc
     1261 ttcgcggagt acgttggaac ccgacatgcg gtggccttcg ccaacggaac ggccgccctc
     1321 cacg
//
LOCUS       DAOTHD010000742         1323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_120792_length_1323_cov_2.809937, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000742 DAOTHD010000000
VERSION     DAOTHD010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120792_length_1323_cov_2.809937"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..>1323)
                     /locus_tag="P5219_10990"
     CDS             complement(185..>1323)
                     /locus_tag="P5219_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HRX27335.1"
                     /translation="LDVTPKSGEFHVYRLENADAKVVAEQLSKILSAQASQAATPDGR
                     IPTTVVPDIPSNSLIFAAPPHHFQSLVEIIQKIDTLPRQVLLRGLIAEVNLTKLNNAG
                     IDWATWGGSVAGDAVIAGQLNMGGSGVPGTIIDWFQNLSRTEEEYRRDDGTYGTRTNY
                     KGMGLVYAYLDLLKKFDAVNVLSMPRIMCTDNLPSELQVGQVIPQLKGKLSDMSNPNA
                     VQSSYEYKDTGLILNVTPSIRSGNLVALDVEQVIEDVLTAMTAETPVTGKRKIKTSVL
                     VSSGNTVILGGLIKEVEKSIRSRVPGLSYIPLIGGLFTRSVKQREKIELMIFLTPYIL
                     ETPGEAEQITREIVSGDHGMSPPEEKLWKRLEKEYRESVKQQSK"
BASE COUNT          198 a          414 c          435 g          276 t
ORIGIN      
        1 gcaaggacct gggcagcggg aaagcactcc ggcgaagcca gggcgaccac aagccgctcc
       61 ccgtcgtccc ggaggggcag gatgcccgcg ttcttcaggg catcgaggct gacccttccc
      121 cggatgaggg gggcgatttc ttcaacgtcg ggaagccgtg gggccattct tccgtctcct
      181 cccgtcattt cgactgctgt ttcaccgact cccggtactc cttctcgagg cgcttccaga
      241 gcttctcctc cggggggctc atgccgtggt ccccggagac gatctccctg gtaatctgct
      301 ccgcctcacc gggcgtctcg agaatgtagg gggtcaggaa gatcatgagc tcgatcttct
      361 cccgctgctt caccgaccgg gtgaagagcc cgccgatgag ggggatgtag gagagcccgg
      421 gaaccctgct ccggatgctc ttctcaactt ccttgatgag cccccccagg atcaccgtgt
      481 tgccggagct caccaggacg ctggtcttga tcttccgctt ccccgtcacc ggcgtctcgg
      541 cggtcatggc ggtgagcacg tcctcgatga cctgctccac gtcgagggcc acgaggttgc
      601 cgctgcggat ggagggagtg acgttgagga tcaggcccgt gtccttgtac tcgtagctgc
      661 tctgcacggc gttcgggttg gacatgtccg agagcttgcc cttgagctgg gggatcacct
      721 gtcccacctg gagctcgctc gggaggttgt cggtgcacat gatccggggc atggagagca
      781 cgttcaccgc gtcaaacttc ttgagcaggt cgaggtaggc gtagacgagg cccatgccct
      841 tgtagttcgt ccgcgtcccg taggttccgt cgtcccttct gtattcctcc tcggtgcggg
      901 agaggttctg gaaccagtcg atgatcgtcc ccggcactcc gctgccgccc atgttcagct
      961 ggcctgcgat aacagcgtcc ccggcgacgg atcctcccca cgtcgcccag tcgatgccgg
     1021 cgttgttcag cttggtcagg ttcacctcgg cgatgagccc ccggaggagc acctgccggg
     1081 gcagggtgtc gatcttctgg atgatctcca ccagggactg gaaatgatgg gggggtgcgg
     1141 cgaagatgag gctgttggac gggatgtcgg gcaccaccgt ggtggggatc ctcccatcgg
     1201 gcgtggcggc ctgggatgcc tgggcggaga gtatcttgga gagctgctcg gccaccacct
     1261 tggcgtcggc gttctccagc ctgtagacgt ggaattcccc cgacttcggg gtcacgtcca
     1321 ggt
//
LOCUS       DAOTHD010000743         1321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_121084_length_1321_cov_2.676145, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000743 DAOTHD010000000
VERSION     DAOTHD010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121084_length_1321_cov_2.676145"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="P5219_10995"
     CDS             <1..297
                     /locus_tag="P5219_10995"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRX27336.1"
                     /translation="LLKEPPALLKDIPTAHESGCPELILNTLYACLAPRGTSPEIVRA
                     LNAAMMDISANSEEYKKEVNRYNLQEPWTLSVEDSVQELKNQRELFMKYSKFLQ"
     gene            412..1119
                     /locus_tag="P5219_11000"
     CDS             412..1119
                     /locus_tag="P5219_11000"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRX27337.1"
                     /translation="MSDRRRALAKELFEHLNSGKIQVNPEGKLPSERDMAAALGVTRP
                     LLRQAIAVLEAFGLLEIRDRQGIFMSRHELPELIFSGDWPPSMMLEIFQVRMMIEPEA
                     ARIVAENRTPEDLRKIGESVAQMGAIIESGREDMESLLSKWNRIFHAQIMAATGNQFL
                     CRIYETVSQAYETASTSLIFRREELRFDEIFSEHRHILDAIREKNGERAAKLVLHHIQ
                     ASVERASESLVSSGFKI"
BASE COUNT          308 a          405 c          376 g          232 t
ORIGIN      
        1 ctcctgaagg aaccccccgc actgctgaag gacattccca cggcccacga gtcgggctgc
       61 cccgaactca tcctcaacac tctctacgcc tgtctcgctc ccaggggaac ctcccctgag
      121 atagtcaggg ccctcaatgc cgccatgatg gacatctctg ccaacagcga agaatacaag
      181 aaggaagtca acaggtacaa cctgcaggag ccgtggaccc tgagcgtcga ggattccgtc
      241 caggagctga agaaccagcg ggagctcttc atgaagtact caaagttcct gcagtaggcc
      301 gttcgaacgg gagaaagaat accttataat aagcacaaca ggacaaattc agccggggcg
      361 gttccaccgc cccggctcct acagatccgg acaaacgggg cagggatcac gatgagtgac
      421 agaagacgag cgctggcaaa ggaactcttc gagcatctga attcggggaa gatacaggtg
      481 aacccggagg gaaaactccc ttcagaacgg gacatggccg cagccctggg cgtcacgagg
      541 ccccttctcc gacaggccat cgccgtgctc gaagccttcg ggctgctgga aatacgggac
      601 aggcagggaa tcttcatgtc ccggcacgaa ctgccggagc tcatcttttc gggtgactgg
      661 ccgccttcca tgatgctgga gatctttcag gtccgcatga tgatcgagcc tgaagccgcg
      721 agaatcgtgg cggagaaccg tactccggag gacctgcgga agatcgggga gtccgtcgct
      781 cagatggggg ccatcatcga gtccggcagg gaagacatgg aatcgctcct ttccaagtgg
      841 aacaggatct tccatgccca gatcatggcg gcgaccggaa accagtttct ctgccgcatc
      901 tacgaaacgg tctcccaggc ctacgagacg gcgagcacat cactgatctt tcggcgggag
      961 gaactccgct tcgacgagat tttcagcgag cacaggcaca tcctggacgc catcagggag
     1021 aagaacggcg aaagggccgc aaaacttgtg ctccaccaca tccaggcctc ggtggaacgg
     1081 gcttcggaaa gccttgtgag ctccgggttc aagatctgaa agcacgaaag cctctccggc
     1141 attgaaccgg ggaggctttt ttcacctggg aagcattcgt ctgcattccc cttcccggag
     1201 gagcatcggt tctgcttacg cgccgccccc gtcctgcagg gagacaaaag tccccccgca
     1261 caccgccgcc aggtcaccgg gggctatttc catatccagc cccctttttc ccgcgctgac
     1321 g
//
LOCUS       DAOTHD010000744         1321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_121142_length_1321_cov_1.873618, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000744 DAOTHD010000000
VERSION     DAOTHD010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121142_length_1321_cov_1.873618"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="P5219_11005"
     CDS             <1..700
                     /locus_tag="P5219_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27338.1"
                     /translation="NEDRLVVITDGAEQMHLLAFWRDEIPAGGERLAGSIPVTCGFDE
                     LPEWIQSEQSVVVSGYGAFVVNNLPGTVDPDLPGKNKILQVALMGPAYPTSYGVERYE
                     WNPENDEWSSLWARPDVSSTSMIPVHSQSGGMVLVNGYTPGNGWEITGLDWNTGRTVH
                     QTIFGRSNHGNGAYAILQYLEDGELLFNAFSGPIRVQYDTGGSGGCSAGGSFAPLPVL
                     LLVPLALLAAGRKK"
     gene            complement(779..>1321)
                     /locus_tag="P5219_11010"
     CDS             complement(779..>1321)
                     /locus_tag="P5219_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRX27339.1"
                     /translation="TVSALNGDSVSEAEREIKAILRQRHRLSERETDDFDVRNMAEML
                     DAAAQSTKVMSLLLGAVASVSLLVGGIGIMNIMLVSVTERTREIGIRMAIGARGSDIR
                     TQFLMEALLLSVAGGIIGIVLGTSASKIITGILGWATVVSTGSIVLAFGFSVFVGIFF
                     GFYPAWKASLLNPIEALRYE"
BASE COUNT          260 a          391 c          437 g          233 t
ORIGIN      
        1 aaacgaggac aggctggttg tcataaccga cggtgctgaa cagatgcatc ttctcgcctt
       61 ctggcgggat gagattccgg ccggagggga gcggctggcg ggcagcatac ccgtgacctg
      121 cggttttgac gagcttccgg aatggataca gtcggagcag tcggtggtag tatcgggata
      181 cggggccttt gtggtgaaca acctgcccgg gacggtggac cccgacctgc cggggaaaaa
      241 caagatcctc caggtcgcgc tcatggggcc cgcctaccct acatcctacg gtgtggagcg
      301 ctatgaatgg aaccctgaaa acgacgaatg gtccagtctc tgggcacgcc ccgacgtctc
      361 ttccacgagc atgattcccg tccacagcca gtctggggga atggtactcg tcaacgggta
      421 cacccccggg aacggttggg aaataacggg gctcgactgg aacacgggca ggacggtgca
      481 ccagaccata ttcggccggt ccaaccacgg aaacggcgcc tatgccatcc tgcagtacct
      541 cgaagacgga gagcttctct tcaatgcctt cagcggcccc atacgggtcc agtacgatac
      601 ggggggaagc ggcgggtgct ctgccggcgg atccttcgcc ccgttgccgg tgttgctcct
      661 tgtgccgctg gcccttctcg cggcgggccg gaaaaagtag accgcctgcg gaactgagcg
      721 accatgacaa aggagctccc ctcaattgag aggggagctc ctttcatttc ggttttgcct
      781 attcgtaccg cagggcttcg atggggttaa ggagggaggc cttccaggcg gggtagaaac
      841 cgaagaagat gcccacgaaa acggagaagc cgaaggccag cacgatggat ccggtggaga
      901 cgacggtggc ccagccgaga atcccggtga tgatcttcga tgccgacgtg ccgaggacga
      961 tgccgatgat cccccccgcc acggagagga gcagggcctc catgaggaac tgggttcgga
     1021 tgtccgatcc ccgggcgccg atggccatgc ggatgccgat ctccctggtg cgttccgtca
     1081 cggagacgag catgatgttc atgatgccga tgccgcccac gagcagggag acggaggcca
     1141 ccgccccgag gagcagggac atgaccttgg tggactgggc cgccgcatcg agcatctccg
     1201 ccatgttccg cacgtcgaag tcgtcggttt ccctttcgga gagccggtgc cgctgccgga
     1261 ggatggcctt gatctcccgt tccgcctcgg acacggagtc gccgttcagg gccgagacgg
     1321 t
//
LOCUS       DAOTHD010000745         1319 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_121361_length_1319_cov_2.875000, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000745 DAOTHD010000000
VERSION     DAOTHD010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121361_length_1319_cov_2.875000"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /gene="purD"
                     /locus_tag="P5219_11015"
     CDS             <1..817
                     /gene="purD"
                     /locus_tag="P5219_11015"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRX27340.1"
                     /translation="SMEEAIQAARRMIEEGILGESGRKVIVEDALEGEELTVLAVTDG
                     RTWRMLPPSQDHKRVFDGDAGPNTGGMGAYAPVPWAGKSLLDRIQKMILEPTFLGLKA
                     EGVPYCGILYAGLMIGPDGMPRVIEYNVRFGDPEAQVVLPLLEGDFGEMIIACCEGRL
                     SDFSYREPLRWAADVVLASGGYPGPFEKGKIISGLDKAAKMQNFVVFHGGTALDPEGR
                     LITSGGRVLSAVGLGNTLEEAIAKAYEGASEICFENIHFRKDIGRKAFLRGRN"
     gene            820..>1319
                     /gene="purE"
                     /locus_tag="P5219_11020"
     CDS             820..>1319
                     /gene="purE"
                     /locus_tag="P5219_11020"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRX27341.1"
                     /translation="MSDERSTPEIGILLGSKSDLALVKKVEDVFSYFGVTWEISVASA
                     HRTPEDVARYASRARERGIRVLVAAAGLSAALPGVVAAHTTLPVIGIPVNAGSVGGID
                     ALLAVAQMPPGVPVASVGIDGVKNAALFAVRILGLHREDIAARLGEWSEKEKEGVRSV
                     RKDLGGL"
BASE COUNT          295 a          346 c          424 g          254 t
ORIGIN      
        1 ttccatggaa gaagcgatac aggccgcccg gagaatgatc gaagagggca tccttgggga
       61 atcgggcagg aaggtcatcg tggaagacgc cctggaggga gaagaactga ccgtcctcgc
      121 cgtcaccgac gggcggacct ggaggatgct tcccccaagc caggatcaca aaagggtttt
      181 tgacggcgat gccgggccga acaccggcgg aatgggtgcc tacgcccccg taccctgggc
      241 cggcaaatcc ctgctcgaca ggattcaaaa gatgattctc gagcccacat tccttggcct
      301 gaaggcggaa ggcgtaccgt actgcggcat tctctacgcg ggattgatga taggtccgga
      361 cggaatgccc agggtcatcg agtacaacgt ccggttcgga gaccccgaag cccaggttgt
      421 gcttcctctt cttgaaggag atttcgggga gatgatcatc gcctgctgcg agggaaggct
      481 gagcgatttt tcgtaccgtg agccccttcg ctgggctgcc gacgtggttc tggcttcggg
      541 aggatatccc ggtccattcg aaaaaggaaa aataatatca gggctggaca aagcggcgaa
      601 aatgcagaat tttgtcgttt tccacggggg taccgccctg gatccggaag gacgcctcat
      661 cacctccgga ggcagggttc tcagcgccgt aggactggga aacactctgg aggaagccat
      721 cgcaaaggcc tacgaaggag cttcagaaat ctgtttcgag aacatccatt tcagaaagga
      781 catcgggcga aaggcatttc ttcgagggag gaattgaaga tgagtgacga gagatcaaca
      841 cccgaaatcg gcatcctgct cggatcgaaa tccgacctgg cactggtgaa gaaggtcgag
      901 gacgtttttt cctacttcgg cgtgacgtgg gaaatttccg ttgcgtcggc ccacaggacc
      961 ccggaagatg tggcgcgcta cgcctccaga gcgagagaac ggggcatacg ggtcctggtg
     1021 gcggcagcgg ggctttccgc cgcccttccc ggggttgtcg cagcccatac tactcttccc
     1081 gtgatcggaa ttccggtgaa tgcggggtcc gtaggaggaa ttgacgccct ccttgctgtg
     1141 gcccagatgc cacccggcgt tcccgtcgca tcggtgggaa tcgacggggt aaagaacgcc
     1201 gcccttttcg ctgtaaggat actgggcctt cacagggagg atatcgcggc caggctcgga
     1261 gaatggtctg aaaaggaaaa ggaaggggtc cgctcggtaa ggaaagatct cggtggtct
//
LOCUS       DAOTHD010000746         1318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_121496_length_1318_cov_2.939826, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000746 DAOTHD010000000
VERSION     DAOTHD010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121496_length_1318_cov_2.939826"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1318)
                     /locus_tag="P5219_11025"
     CDS             complement(<1..>1318)
                     /locus_tag="P5219_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRX27342.1"
                     /translation="KAELSRHEVFTTARTRHPMRVRIKTGVKKDGTITALEMNDIMNT
                     GAYGAHALTVLSNAGSKVLPMFNKIPNVSFTGRAVYTTLPVGGAYRGYGATQGYFAFN
                     QHVDSICRKLGLDFVDYVKKWHIREGETSEVFKAIGEGTEGVSQIIKSCKLSECLDRG
                     AEEIGWSRLRGKRITSGSWVKGVGAAVSMQGSGIPKVDMGSASMKMNDDGSFNLYVGA
                     TDIGTGSDTILSQIAAETLKVPVEMILILSSDTDLTPFDTGAYASSTTYVSGQAVLRC
                     AGKIRDQILSAAAVLMGASADSLSLGDGGVVVNRKSGEEAPFSKIACFAMYSHDQFQI
                     QAGASYTGDESPAPFMAHFAEVDVDLETGKVRLVKFVSAGDCGRPINPVLAEGQIEGA
                     TMNGISYALTEQYLFDPRGKMTNSSFWDYKIFGTLDMPEMKTILAES"
BASE COUNT          254 a          392 c          376 g          296 t
ORIGIN      
        1 cgattccgcc aggatggtct tcatttccgg catgtccagg gtaccgaaaa tcttataatc
       61 ccagaaggag ctgttcgtca ttttccccct cgggtcgaag agatactgtt ccgtgagggc
      121 gtagcttatg ccgttcatgg tggccccctc gatctgaccc tcggcgagga cggggttgat
      181 cggcctcccg cagtctccgg ccgagacgaa cttcaccagg cggacctttc ctgtttccag
      241 gtccacgtcc acctcggcaa agtgagccat aaagggcgcc ggcgattcat ctcccgtata
      301 ggaggcgcct gcctggatct ggaactggtc gtggctgtac atggcgaaac aggcgatttt
      361 cgagaagggg gcttcctctc cggatttcct gttcaccacg acccctccgt caccgaggga
      421 aagggaatca gccgaggcac ccatgagaac ggcggcagcg gacagaatct ggtccctgat
      481 ctttccggcg cagcggagca ccgcctggcc cgaaacgtag gtggtagagg aagcgtatgc
      541 cccggtgtca aagggcgtaa gatccgtgtc ggaggaaaga ataaggatca tttccacggg
      601 aaccttcagc gtttccgccg cgatctggct gaggatggtg tcagaccccg tgccgatatc
      661 cgtagccccc acatacaggt tgaaagaacc gtcgtcgttc atcttcatgg aagcgcttcc
      721 catgtccact ttcgggatgc ccgaaccctg cattgaaacg gcggcgccca cacccttgac
      781 ccacgagccg gaggtgatcc tctttccccg gagcctgctc cagccgattt cctcggcccc
      841 gcggtccagg cactccgaaa gcttgcacga cttgatgatc tggctgaccc cttcggttcc
      901 ttctccgatg gccttgaaaa cttcggaggt ttcaccctcg cggatatgcc acttcttcac
      961 gtagtcaacg aaatccaggc cgagttttct gcagatgctg tccacgtgct gattaaaggc
     1021 aaaatacccc tgggtggcgc catatccccg ataggctcct ccaaccggga gggtggtgta
     1081 gaccgctctt ccggtgaagg agacattggg tatcttgttg aacatgggca gaaccttcga
     1141 tcccgcgttg gagagcaccg tcagggcatg ggcaccgtag gcgccggtat tcatgatgtc
     1201 gttcatttcc agggcggtta tggtgccgtc cttctttacg ccggtcttta tgcgtaccct
     1261 catgggatgc ctggttctcg cggtggtgaa gacttcatgg cgcgaaagct cggccttt
//
LOCUS       DAOTHD010000747         1317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_121630_length_1317_cov_2.641838, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000747 DAOTHD010000000
VERSION     DAOTHD010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121630_length_1317_cov_2.641838"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="P5219_11030"
     CDS             <1..261
                     /locus_tag="P5219_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27343.1"
                     /translation="TYVPGAEIYVTPPEKSPFAVEYVRFLPGTVFPESMHYDVHVAAK
                     VDSIEKYAALADEIIVPKMDGGACWLCFVRKDGAIIELMEEK"
     gene            complement(330..>1317)
                     /locus_tag="P5219_11035"
     CDS             complement(330..>1317)
                     /locus_tag="P5219_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRX27344.1"
                     /translation="LSYSVYMGLAFFFILFTAFVALDLARLVAWLADTLFSTDLRRLL
                     PSPKSRTLGAVGLALLVCAYGWFEALNVHPRHVTIETERLPAGTDRIRVAHITDVHLG
                     WIIQEERLDRILSVVRAAEPDLVVSTGDLVDDNMEFRDEEIALLRGLTPPLGTYAVTG
                     NHEYHVGVRQAVEFKERAGMKVLRNEHHSVGGLVLVGMDDPSARYYGEGIPPEGDILA
                     SLPDDRFVVLLKHQPRVEPESVGLFDLQLSGHTHGGQIWPFYWLTRMVYEYRPGLRAL
                     APSPRGGEAYADENSRESSIYVSNGAGTWGPPLRFFAPPEVTIFNIVRKPGS"
BASE COUNT          249 a          403 c          416 g          249 t
ORIGIN      
        1 acctatgtcc ccggggcgga gatctacgtc actccgcccg aaaagtcacc cttcgccgtg
       61 gaatacgtcc gtttccttcc cgggacggtc ttccccgagt ccatgcacta tgatgtgcac
      121 gtggccgcga aggtcgattc catcgagaag tacgcggccc tggccgacga gatcattgtc
      181 ccgaagatgg acggaggcgc ctgctggctc tgcttcgtcc ggaaggacgg cgccatcatc
      241 gagcttatgg aggagaaata gcggcgccgg gacggagtcc tctcttcggc cgaaggggct
      301 gtccggaggc tgaaagggct gttttccctt cagctccccg gttttcttac gatgttgaaa
      361 attgtcactt ccggcggagc gaagaaccgg aggggcggcc cccaggttcc agcaccgttg
      421 cttacgtaaa tggagctttc cctggaattt tcatccgcgt aggcttctcc tccccttgga
      481 gacggtgcca gggcccgcag gccgggacgg tattcataga ccatgcgggt gagccagtag
      541 aagggccaga tctgccctcc gtgggtgtgg cccgagagct ggaggtcaaa gagccccacc
      601 gattcgggct ccactctcgg ctggtgcttc agcagcacca cgaatcggtc gtcgggcagg
      661 gaagcaagaa tatccccctc ggggggaatc ccttcgccgt aataccgtgc cgaggggtcg
      721 tccatgccca cgaggaccag tccgcccacg gaatggtgtt cgttccgcag gactttcatg
      781 ccggcccgct ccttgaattc aacggcctgc cggacgccca cgtggtactc atggttgccg
      841 gtgactgcat aggttccgag gggcggggtc aatcccctga ggagggcgat ctcctcgtcc
      901 cgaaactcca tgttgtcgtc cacgaggtct ccggtgctga ctaccaggtc cggctccgcc
      961 gcccggacca cggagaggat gcggtcgagg cgctcctcct ggatgatcca tcccaggtgc
     1021 acgtcggtga tatgggctac gcggatccgg tccgttccgg ccggaagccg ttccgtttcg
     1081 atggtgacat gcctcggatg tacgttgagg gcttcaaacc agccgtaggc gcagaccaga
     1141 agagctaagc ccactgcccc caatgtcctg ctcttcggag agggaagcag ccgcctcagg
     1201 tccgtggaga acagggtgtc ggccagccat gccacaagcc tggccagatc gagagcgacg
     1261 aaggccgtga agagaatgaa gaagaaggcc agccccatgt acacggagta ggacagc
//
LOCUS       DAOTHD010000748         1314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_122068_length_1314_cov_2.049245, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000748 DAOTHD010000000
VERSION     DAOTHD010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122068_length_1314_cov_2.049245"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1314
                     /locus_tag="P5219_11040"
     CDS             <1..>1314
                     /locus_tag="P5219_11040"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRX27345.1"
                     /translation="IVNSLLRGFIKAEGKAPAGFLLFGDAGIGKTRILAQFMEKIRQF
                     PGNPPVILLARAKKFGPAPFSLINELLLSYLHAQQRTSGRSMEDELLAGLGLDAETVS
                     RFLSLLAGGALPDDESGSFVVLYLILVAILRKHRESPYAPLLAIDNCHNIDPQSLDFF
                     RFFLKSTEIHPFLLLAGRNENRVIQDVFASLSVIDVPPLGAGESHELFKALWEEEEAQ
                     GMEEKILEYAAGNPLFIEEYVKYAEEHADIGLLPDTIQNIYLSAIEKLESGTRDILRK
                     VSVFIHNFTFDDAAYIEEHTEGDVGTLGERLERLVGERYLYKENGFFHFKSDAMKRAL
                     YNALLNHNKKILHRLVSERLKGQKAPNILRLIHHLIRAEEYTEAGEALVRDPGYIQKM
                     EYLPYLDSLIAHARGKSDSDFFRFMFTKSGILFNNGKKGESEEVL"
BASE COUNT          297 a          381 c          362 g          274 t
ORIGIN      
        1 gatcgtcaat tccctgctcc ggggcttcat caaggcggag ggcaaggctc ccgcggggtt
       61 ccttctcttt ggcgacgcgg gaatcggaaa gacccggatc cttgcccagt tcatggagaa
      121 aatccggcag tttccgggca acccccccgt tattctcctt gcccgggcga agaaattcgg
      181 ccccgcgccc ttttccctga taaacgagct gctgctgagc tatcttcacg cccagcagcg
      241 gaccagcggc cgctccatgg aggatgagct cctggccggt ctggggctgg acgcggagac
      301 ggtgagcagg ttcctctccc ttctggccgg gggggccttg cctgacgatg aatccggcag
      361 cttcgtcgtc ctctatctca tcctggtggc gattctccgg aagcatcggg agagtccgta
      421 tgcccccctt ctcgccatcg acaactgcca caatatcgat ccccagagcc tcgatttttt
      481 ccggttcttc ctgaaaagca cggaaattca tccctttctc ctcctggcag ggaggaacga
      541 gaaccgggtg atacaggacg tcttcgcctc gctgtccgtc atcgatgtcc ccccccttgg
      601 cgcgggagag agtcacgagc ttttcaaggc tctgtgggag gaagaggaag cgcagggaat
      661 ggaagagaag attctcgaat acgcagccgg gaatcccctt tttatagagg aatacgtgaa
      721 atacgcggaa gagcacgccg atatcggcct tctgccggac acgatccaga acatctatct
      781 ttccgccatt gaaaaactgg aatcaggaac ccgtgacatt ctaagaaagg tgtcggtatt
      841 catccacaat ttcaccttcg atgacgccgc gtacatcgaa gagcataccg aaggagatgt
      901 cgggacgctc ggggaacggc tggaacggct tgtcggggaa cgatatctct acaaggaaaa
      961 cgggtttttc catttcaaga gcgacgccat gaaaagggcc ctctataatg cccttctcaa
     1021 tcacaacaag aagatccttc accggctggt atcggagcgg ctcaaggggc agaaagcccc
     1081 caatattctg cgcctcatcc atcatcttat cagggcggag gaatacaccg aggccgggga
     1141 ggcccttgtc cgggaccccg gatacatcca gaagatggaa tatctcccct atctcgacag
     1201 cctcatcgcc catgcccggg ggaagagcga ctccgatttt ttccggttca tgttcaccaa
     1261 atcggggata ctgttcaaca acggaaagaa gggggaatcc gaagaggttc tcga
//
LOCUS       DAOTHD010000749         1309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_122706_length_1309_cov_5.078150, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000749 DAOTHD010000000
VERSION     DAOTHD010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122706_length_1309_cov_5.078150"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="P5219_11045"
     CDS             <1..766
                     /locus_tag="P5219_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRX27346.1"
                     /translation="MLLAANLLFIRFLPGTAGARLSFLSVALWWALFSVPVFLFVDEP
                     PPSPEGKDLSGTALFLKGLERLRMTFREIRHYRNLFVFLLAFWLYNDGIGTIIRMAAI
                     YGATLGIPMSHLVGSLLVTQFVGIPFSLAFGSLASKIGSKRSILAGLFFYLCITFAAI
                     FVREIWHFWALAIAVGMVQGGTQAMSRSFFASMIPPGRTAEFFGFYDISSKFSGIFGP
                     AVFGLVTQATGSSRLAIAVLSYTFILGILFLRKVRG"
     gene            796..>1309
                     /locus_tag="P5219_11050"
     CDS             796..>1309
                     /locus_tag="P5219_11050"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRX27347.1"
                     /translation="MIPLVALLLCAISPGLEGATVLVSGSSLVRTVAITFDDGPHDIL
                     TPTLLDVLDTLGVKASFFLVGSMAEKKPDLVREIRSGGHTVANHSQTHRSCLVLSAEE
                     LEDEIRKCSAVLESVTSVPVRFFRPPGGKFDRKTVERVRKNGLKLVLWDINSRDYTGV
                     SPSYIANRAVR"
BASE COUNT          249 a          388 c          333 g          339 t
ORIGIN      
        1 gatgctcctt gcggcaaatc ttctcttcat ccgctttctt ccgggaaccg ccggggcacg
       61 gctttccttt ctctccgtcg cactgtggtg ggctcttttt tccgtccccg tctttctttt
      121 cgtggacgaa ccgccccctt cgccggaagg gaaggacctg tccggaacgg ccctgttcct
      181 gaagggcctt gaaaggctca ggatgacttt cagggagatc aggcactaca gaaacctttt
      241 tgtttttctc ctggctttct ggctgtacaa cgacggcata ggcaccatca tcagaatggc
      301 cgcaatctat ggggccaccc tgggcattcc catgtcccac ctggtcggct ctcttctggt
      361 cacccagttc gtgggtattc ccttttccct cgctttcggc tctctcgctt cgaaaatcgg
      421 cagcaaacga tcgatcctcg cagggctttt cttctacctc tgcattacct tcgcagccat
      481 tttcgtccgt gaaatatggc atttctgggc ccttgccatt gcggtgggaa tggttcaggg
      541 aggtacccag gcaatgagcc ggagcttttt cgcttcgatg atacctccgg gaagaacagc
      601 cgaatttttc gggttctacg atatttccag caaattttcc ggcatattcg gccctgcagt
      661 cttcggactc gtcacccagg ccaccggatc aagcaggctt gccatcgccg ttctctccta
      721 cacctttatt ctcggcatcc ttttcctcag aaaagtgaga ggatgatatc cttgccgttc
      781 cgctgcagac tgcagttgat tccgcttgtc gcccttctcc tttgcgccat ttcacccggt
      841 cttgaggggg cgacggtgct cgtatccgga agcagccttg tcaggacagt ggccataacc
      901 ttcgatgacg gcccccacga catacttacg cccacgctgc tcgatgttct cgacaccctc
      961 ggcgtgaagg caagcttttt tcttgtgggt tccatggccg aaaaaaagcc tgacctcgtc
     1021 cgggagattc gctcaggagg gcatactgta gcgaatcatt cccagactca caggagctgc
     1081 cttgtccttt cggcggaaga gctcgaggat gaaatccgga aatgctcggc cgtactcgaa
     1141 agcgttacgt ccgttccggt ccgttttttc agaccaccgg gcggaaaatt cgacaggaag
     1201 acggtggaaa gagtcagaaa gaacggtctg aaactggtgc tctgggacat caacagccgg
     1261 gactacacgg gagtgtctcc ctcctatata gctaacaggg cagtccgta
//
LOCUS       DAOTHD010000750         1308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_122846_length_1308_cov_4.185156, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000750 DAOTHD010000000
VERSION     DAOTHD010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122846_length_1308_cov_4.185156"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="P5219_11055"
     CDS             complement(<1..241)
                     /locus_tag="P5219_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRX27348.1"
                     /translation="MKKIQTLFSVLTGICLAVVFAVTFGQVIQRYVFQLPMPWATDII
                     RIFFVYSVFFGMAVGVFKKAHLNIDVLVQVLPPRLK"
     gene            complement(307..1296)
                     /locus_tag="P5219_11060"
     CDS             complement(307..1296)
                     /locus_tag="P5219_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRX27349.1"
                     /translation="MKKSGVFLAAVAVCMILLTGTAFAAVEFKFAHSGSLEHQYQIGA
                     EQFKKLVEEKSGGEMKVTIFPQAQLGGERDLAEGVRMGTIEMSSVAAGNMAGFVPELQ
                     VFGVPFLFQTREQVYAVLDGPVGKDLADIMLGKGFVNLSIWEVGFRNITNNTRPVKTP
                     EDMKGLKIRVQESKIWIEFMKRLGAIATPIPFGELYTALQQKVVDGQENPVATIYSMK
                     FYEVQKYLSLTGHTYEPALVVANPKWFNGLDPKHQAILKEAAMEAAAFQRQKLADLDR
                     ERFDVIRKAGVEVEENPDKAAFAKATEDLHKVLADSVPEALVQKIKDEVAKVK"
BASE COUNT          259 a          404 c          375 g          270 t
ORIGIN      
        1 gcttgagccg cggaggcagg acctggacca gcacgtcgat gttgagatgg gcttttttga
       61 acacccccac cgccatgccg aaaaagacgg agtagacgaa gaaaatcctg ataatgtccg
      121 tcgcccaggg catggggagc tggaacacat accgctggat cacctggccg aaggtcacgg
      181 caaagactac cgcaagacag attcccgtga gaacggaaaa cagcgtctgt atttttttca
      241 cttgactcca cctcgagctc tcgaaaaaaa agctccgggg gaaagctccc ccggagccgc
      301 ggggcactac ttcacctttg cgacttcatc ctttatcttc tggacaagag cctcgggaac
      361 ggagtcggcc agcaccttgt gaagatcctc ggtagccttg gcgaaagccg ccttgtcggg
      421 attctcctcc acttccacgc cggccttcct gatgacatcg aaacgttccc tgtcgaggtc
      481 ggcaagtttc tgccgctgga aggcagccgc ttccatggcc gcttccttca ggatggcctg
      541 gtgcttcggg tcgaggccgt tgaaccactt gggattggcc accacgaggg cgggctcgta
      601 ggtgtggccc gtcagggaga ggtacttctg cacctcgtag aacttcatgg agtaaatggt
      661 ggccacggga ttctcctggc cgtcgacaac cttctgctgc agggcggtgt acagttcacc
      721 gaaggggatg ggggtggcga tggcaccaag gcgcttcatg aattcgatcc agatcttgga
      781 ctcctggacc cggatcttca ggcccttcat gtcctcggga gtcttcacgg gccgggtgtt
      841 gttggtgatg ttcctgaagc ccacctccca gatggagagg ttcacgaaac ccttgccgag
      901 catgatgtcc gccaggtcct tgcccacggg gccgtcaagg acggcataga cctgctccct
      961 ggtctggaag aggaaaggca cgccgaaaac ctggagctcg gggacgaatc ccgccatgtt
     1021 ccccgccgcc acggaactca tctcgatggt gcccatgcgg acgccttcgg ccaggtcacg
     1081 ttcgcccccg agctgggcct gggggaagat ggtgaccttc atctcgccgc cggacttctc
     1141 ttcgaccagc ttcttgaact gctccgcgcc gatctggtac tgatgctcca ggcttcccga
     1201 gtgggcgaac ttgaactcca ccgccgcgaa cgccgtcccg gtcagcagaa tcatgcacac
     1261 cgcaaccgcc gcaagaaaca ctccgctttt cttcattttc ttcccctc
//
LOCUS       DAOTHD010000751         1308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_122900_length_1308_cov_2.574621, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000751 DAOTHD010000000
VERSION     DAOTHD010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122900_length_1308_cov_2.574621"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="P5219_11065"
     CDS             complement(<1..278)
                     /locus_tag="P5219_11065"
                     /inference="COORDINATES: protein motif:HMM:NF014601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HRX27350.1"
                     /translation="MKIVFVGLGGVGGYYGGKTARAFEGGSEHRVMFLARGEHLRTIS
                     EKGLKVYAQDEEFVARPFLATDDPGEMGTADLVVFAVKGYDLRAAARAA"
     gene            complement(315..872)
                     /gene="msrA"
                     /locus_tag="P5219_11070"
                     /pseudo
     CDS             complement(315..872)
                     /gene="msrA"
                     /locus_tag="P5219_11070"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
     gene            complement(888..1019)
                     /locus_tag="P5219_11075"
     CDS             complement(888..1019)
                     /locus_tag="P5219_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27351.1"
                     /translation="MKMFEAVIAAAVVFFAGRACAGEIPPLDRQIPSELRTAAFALG"
BASE COUNT          266 a          415 c          347 g          280 t
ORIGIN      
        1 gccgcacggg cggccgcacg gaggtcgtac cccttgacgg caaacaccac aagatccgcc
       61 gtccccattt ccccaggatc gtcggtggcc aggaaaggcc gggcgacaaa ttcctcgtcc
      121 tgggcataca ctttcagacc cttttccgat atggtcctga ggtgctctcc ccttgcgaga
      181 aacatcaccc ggtgctcact gccgccttcg aacgcccggg cagtcttgcc gccatagtac
      241 ccgcccactc cgccgagacc cacaaacacg attttcatcc tgttctttcc tctccccttc
      301 ctgacgaatg cctcttattt cccccttgta cggagtcttt tctgtgcctc ctccgaaaga
      361 cccagcagag gaaggaatgt ttccagttcc tcctttgagg ccagtcccgc aataattccg
      421 ttggccttcg cggccgaggg ggaactcaca aaaccgtccc gctccgggaa catttcattg
      481 aactccctca tgagaacata gtccctgcgg aggtaatact tctggtgata cccttccgcc
      541 gggtaaaacc gggccagggg ttcaatggcc gtgaagattt ttccgcctat gtccgcctca
      601 atcttcgcgg ccgaacgttc cgcccttttc cgctggtcat cgtccgccgg aaagatgacg
      661 gagcggtact gggtcgacca tgggcgcgca cctggattgt ggctcgtcca gaagacgtcc
      721 aggagttcgt cgtaggagat cttttccggg tcgtaatcaa cctggacggt ctcggcatgg
      781 tctccgatgt catcataggt gggatcgggc gttgttccgc cggcataccc cacccgggtg
      841 cgcaccactc cgtcgagaag cccgaaccgg gcatcagggc cccagaatca gccaagggca
      901 aaggcggccg tccggagttc cgaagggatc tgccggtcca ggggaggaat ctccccggca
      961 caggcccttc ccgcgaaaaa taccaccgca gcggcaatga cagcttcaaa cattttcatt
     1021 ctctccacct cccgaaaatt tccttatcat tattttacac taccgtcatg tttctttacg
     1081 gaacggattg tgtccgggag atttccctgc tgtacaatgg aaaccataca tgcacctgaa
     1141 ggaggcgcgg cacacgatat gaaggaaaca tcagtggatt tctacttttt cagcggaacg
     1201 ggaaacaccc tgctcgcagc ccgggcagtg acccgcaggc tgcgggaggg aggaaaggac
     1261 gtccggctcc ggcggctgga aaaggggttc gttcccttcc gtgacggc
//
LOCUS       DAOTHD010000752         1308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_122957_length_1308_cov_1.899441, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000752 DAOTHD010000000
VERSION     DAOTHD010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122957_length_1308_cov_1.899441"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="P5219_11080"
     CDS             <1..295
                     /locus_tag="P5219_11080"
                     /inference="COORDINATES: protein motif:HMM:NF013962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRX27352.1"
                     /translation="LEEVESRYYLRVMVADRPGVFAALASVLGKHGISISSALQKEPP
                     SADGTSVPVPIVITTHHVREGALMAAIRELNELSCVTDKCAFIDILEEQEETI"
     gene            417..>1308
                     /locus_tag="P5219_11085"
     CDS             417..>1308
                     /locus_tag="P5219_11085"
                     /inference="COORDINATES: protein motif:HMM:NF012563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HRX27353.1"
                     /translation="MKEEGCEQKVWSREGHDENSLAWGFAEHLKYSLGVDNYTATDHD
                     RFMSLAYAIRDRLINQWIKTQQTHHDKHAKRVYYLSLEFLIGRSMGMNVLNLGLEDEV
                     EKALESLGYTWEELREQEVDAGLGNGGLGRLAACFLESMATRELPAFGYGLPYDYGIF
                     RQSIENGYQVEQPDDWLRQGNPWEIERTDISPIVRFGGRVEIISRNGKLDAHWLDAQQ
                     VMGIAYDMPVVGYGGRTVNTLRLWSAKATEEFDLQDFNAADYAEAVRSKVNAENLTKV
                     LCPNDRLSLGKELRLKQQYFF"
BASE COUNT          293 a          388 c          402 g          225 t
ORIGIN      
        1 cctggaggag gtggaaagcc ggtactacct ccgggtgatg gtcgccgatc ggccgggagt
       61 tttcgccgcc ctggcgtctg tcctgggaaa acacggcatc agcatctcct ccgccctgca
      121 gaaggaaccc ccctcggcgg acggaacttc cgtccccgta cccatcgtga taacgaccca
      181 tcacgtgcgg gaaggggcgc tgatggcggc gatacgggaa cttaacgagc tttcatgcgt
      241 aacggacaaa tgcgcgttca tagacatcct cgaggagcag gaagaaacca tctgaggccc
      301 gccggccgcg aattccgccg gcagatacga ttgcgcgaag ctgtccgctg gattatcatt
      361 gtatctgcgc cgtcgcacat gccctggcaa cggcgactcc tgatatggag gtggatatga
      421 aagaggaagg ctgcgaacag aaagtgtggt cccgcgaggg gcatgacgaa aattccctgg
      481 cgtggggctt tgcggaacac ctgaaatact ccctgggcgt ggacaattac acggcgaccg
      541 atcacgaccg gttcatgtca ctggcctatg ccatcaggga ccgcctcatc aatcagtgga
      601 taaagacaca gcagacacat cacgacaagc acgcgaaacg ggtctattac ctttccctgg
      661 aattcctcat cggccgatcc atggggatga acgtgctcaa cctcgggctg gaggatgagg
      721 tggaaaaagc cctcgaatcc ctcggctaca cctgggagga actcagggag caggaggttg
      781 acgcggggct ggggaacggc ggcctgggga gactcgccgc ctgcttcctc gaatcgatgg
      841 ccacgcgcga gcttcccgcc ttcggctacg gcctcccgta cgattacggc atattcaggc
      901 agtcgataga gaacgggtat caggtggaac agcctgacga ctggctccgt cagggaaacc
      961 cctgggaaat cgagcggacg gacatctctc ccatcgtccg tttcggaggg cgggtggaaa
     1021 tcatctcccg gaacggcaag ctcgacgccc actggctcga cgcccagcag gtgatgggaa
     1081 tcgcgtatga catgccggtg gtcggctacg gcggcaggac ggtcaacacc ctccgcctct
     1141 ggagcgccaa ggccaccgag gaattcgacc tgcaggactt caacgccgcc gactacgccg
     1201 aggcggtgag gtcaaaggtg aacgcggaaa acctcaccaa ggtcctctgt ccgaacgacc
     1261 gcctctcact gggaaaggag ctgcggctca aacagcaata tttcttcg
//
LOCUS       DAOTHD010000753         1307 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_123051_length_1307_cov_2.678914, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000753 DAOTHD010000000
VERSION     DAOTHD010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123051_length_1307_cov_2.678914"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1216..1303)
                     /locus_tag="P5219_11090"
     tRNA            complement(1216..1303)
                     /locus_tag="P5219_11090"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1267..1269),aa:Ser,seq:gga)
BASE COUNT          272 a          307 c          360 g          368 t
ORIGIN      
        1 ccatcttttt tccctgccgg gcagacagtc gggtgttgaa aatgtccatg agttcggtga
       61 gatgttcctt ggttatttcg gaaacttttc tgccttggcc gaaatagcga agaatgaatt
      121 tgaatttcga ctcaaactct cctcgggtct tttcggtcca gcgaacttcc ttccgggcct
      181 ttgatgtagt cttctcggca ataatttcct gtacgagcgt tgcgatttcc gggtcttcat
      241 agtccggtcc tgcagtggga aggggtgtag tattattcac cgaaactgcc ggcggcacta
      301 ccggatgttc ggatctgtag gcaatttgta cggattccgg cggaagagct ccgttgagtt
      361 gcatcagggc acgatcgcaa ttcgtcagtg ccccgcaggc gaatttttgg gcgatcaacg
      421 ggaaatgcct gtttgtgtgg gggatgagtt tccggacgtt gtaaaggtcg gtaaaagctc
      481 tggccttgag tatgttccgg aaattcttct tccggacgag gagataaagg gtcaggtcct
      541 caacctccat ggatattccg agattgtacc cttcgactgt acggtggtcg gatgccagga
      601 gttttttttc aagaaaggcc cagtcattag cgatgagaag ctccaccatt aagtcgatct
      661 ctcgctggga gaactccgtt tcaggtgatt tttcggaggt gacggggatg attcttcttt
      721 ttccctcgaa aagctcccaa aagttattgt gctgctttgt gtcaccgtac acctcgttga
      781 tcgcttcctt ttccgtctct tcgtcgggag tcagatgaag ggagtggtca cgggccaggg
      841 caaccgattc gacggagtcg tcccaggaag ggttgtactt gtcggtaaga agcagggctt
      901 cctcatgaag ccatttggct ctttgagctc tttcttcagg agtgagatcc atgggcagaa
      961 aaatcaagcc agcgcgaagg tattctcctc tttttcgcgc gagtttgagg tagggggttt
     1021 tgagggagag ccggatttcc gatttgttcc ataggaatcg ctccgttttg ggtatgaccc
     1081 agcggaaata ataaatattc ccgcggcgct cgatgaatgg aacaccagtt tgtaacacca
     1141 agggtgctgt acgcttttgg cgcacagcgt gtataccgtt tttctcttct gtttccagta
     1201 ttaaggcata attaatggcg gagaaggagg gattcgaacc cacggaacct cgcggttcag
     1261 acgctctcca agcgcccgcc ttcgaccact cggccacttc tccgcat
//
LOCUS       DAOTHD010000754         1305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_123320_length_1305_cov_3.176000, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000754 DAOTHD010000000
VERSION     DAOTHD010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123320_length_1305_cov_3.176000"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="P5219_11095"
     CDS             <1..428
                     /locus_tag="P5219_11095"
                     /inference="COORDINATES: protein motif:HMM:NF028178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRX27354.1"
                     /translation="GDHFKDRLVDAFSFTTGAPNPILQDISTLQDLVFIPIDGEMRNK
                     LIEKYPFMAKEVIPGNTYRGQDQPVETVAVQAILAVRKELPEDVVYTMTKALFQNLEA
                     VGNAHHKGKSILLSKALDGISIPVHPGAAKFFKEAGLMK"
     gene            1009..>1305
                     /locus_tag="P5219_11100"
     CDS             1009..>1305
                     /locus_tag="P5219_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter permease"
                     /protein_id="HRX27355.1"
                     /translation="MVENNDVKPQQEIFDSTAAEKAAETIDVEKILEKYDRESTTRTL
                     TPSWTGIVKIICILFSLFQLYTAAFGVYEAQIQRSVHLAFVLVLVYLLYPARLSQ"
BASE COUNT          282 a          355 c          345 g          323 t
ORIGIN      
        1 ctggtgacca cttcaaagac cgcctcgttg acgccttctc cttcaccacc ggcgcaccca
       61 accccatact ccaggacatc agcacgctcc aggatctcgt cttcattccc atcgacggcg
      121 agatgaggaa caagctcatt gaaaagtacc ccttcatggc aaaggaagtc ataccgggaa
      181 acacctaccg cggacaggac cagcccgtgg agaccgttgc tgtccaggcg atcctggcgg
      241 tgcggaagga acttcccgag gatgtggtct acaccatgac gaaggccctt ttccagaacc
      301 ttgaggctgt cggcaatgct caccacaagg gcaagagcat ccttctgagc aaggctcttg
      361 acggcatttc cattcccgtg catccgggcg ctgccaagtt cttcaaggaa gcggggctta
      421 tgaaataggc tgagaaccat tttcgggagg ccttcctgag cggaggcctc ccttcgtgct
      481 tttcctgagg ggggagtggg tgttgaacag aaaaatcctc ttgtactgct tttttctcct
      541 ttcctttctt gtcctgttct gttccccggc ccactttctc atgatcagaa gcgcagacgg
      601 ctcccttctc gccgcatatc ccttgagagt cggcgagacg ttttcggtga ggtacattca
      661 ttccgtacag aaaacgcccg tgctggaagt gttccgtctt gatttccggg agggcatcga
      721 gctgagagaa actgtctaca cggatttcgg ggcggggctg ccgtatcttc tggaaggaaa
      781 cgaggttttt gagagcgggg gggggaaatt ccggatatac ggaataggaa ggcatcttcc
      841 ggacattgtt ttccgtgtcg gccggttcgc ggattaccgt cttctgttcc gcgaaaggga
      901 cattcctttc actcaatttg aagagccggg aaaacctctt cgtttcacag gagagaaagg
      961 tccttatatt ttttcttttt tttattgatt ccctgaggag gaacggtcat ggtggaaaac
     1021 aacgatgtga aaccgcagca ggaaattttt gattcaacag cggcggaaaa ggcagcggag
     1081 acaattgatg tcgaaaagat tctcgaaaaa tatgaccggg agtcgactac ccggacgctc
     1141 accccgtcct ggacgggcat cgtcaagatt atctgcattc ttttctccct cttccagctc
     1201 tataccgcgg ctttcggggt atacgaggcc cagatccagc gttccgtgca ccttgccttt
     1261 gttctcgtcc tcgtgtacct tctgtacccg gccaggctga gccag
//
LOCUS       DAOTHD010000755         1305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_123338_length_1305_cov_2.700800, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000755 DAOTHD010000000
VERSION     DAOTHD010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123338_length_1305_cov_2.700800"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="P5219_11105"
     CDS             complement(<1..933)
                     /locus_tag="P5219_11105"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27356.1"
                     /translation="MSSSGNNPTKGAFGQIRKKGFAALFTEQYPLFRASGVLILISFA
                     LSIVLLRWMITDRATGFILGETSPRSYFALYPMSYLDDEGTRVLRSRVGETVAGVLVR
                     DGAAMDRLIVKIEAMAKGDLAPLSLTTGLAELLKTMPEENRKKILTEAARISSDFLKN
                     AGGDGLPIAPTPETIWKEIEKLSLPMEMNNVIFQILDEILQPLTRIDDDLTETLRGEL
                     AESLQPVERSVAPGDVLIEKGQTVTPQVARILKMQGYSQVTFPWKQILFALLALPFWP
                     LWVLLQTSFRPSARQNIPWLYLSFAVGLSWVVEYF"
     gene            complement(905..>1305)
                     /locus_tag="P5219_11110"
     CDS             complement(905..>1305)
                     /locus_tag="P5219_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRX27357.1"
                     /translation="YDAFYDLLSPERFLRYVDKGVIEIAPLAYMRGRTLNDSFIILDE
                     AQNTTPEQMKMFLTRLGFGSKAVITGDITQVDLPSGKLSGLKQVKDILDGIEGIAFFY
                     LTHSDVVRHEIVQQVVKAYERYEQQREQSH"
BASE COUNT          254 a          356 c          434 g          261 t
ORIGIN      
        1 gaagtattcc accacccagc tcagccctac ggcgaaggag aggtacagcc aggggatgtt
       61 ctgccgcgcc gacggcctga aactggtctg cagaagcacc cagaggggcc agaagggaag
      121 ggcgagcagg gcgaagagaa tctgcttcca ggggaaggtc acctgggagt atccctgcat
      181 tttcagtatc cgtgcaacct ggggcgtcac ggtctgtccc ttttcgatga gcacatcgcc
      241 gggcgccacg gaccgctcca ccggctggag ggattcggca agctcgcccc ggagggtctc
      301 ggtgagatcg tcgtctatcc gggtaagggg ctggaggatt tcatcaagaa tctggaaaat
      361 gacgttgttc atctccatgg gaagggagag tttttctatc tccttccaga tggtttccgg
      421 cgtcggcgcg atgggcaggc cgtcgccgcc ggcattcttg agaaaatcgc tgctgatccg
      481 ggcggcttcg gtgaggattt tcttccggtt ttcctcgggc atggtcttca gcagctcggc
      541 gaggccggtc gtcagggaga ggggggcaag gtcgcccttg gccatggcct ctatcttgac
      601 gataaggcgg tccatggcgg ccccgtcccg tacgaggacg cccgccacgg tctcccccac
      661 cctggagcgg agcacccttg tgccctcgtc gtcaaggtag ctcatgggat aaagggcgaa
      721 gtacgaccgg ggagaggttt cgccgagaat aaagccggtg gcgcggtcgg tgatcatcca
      781 gcgcaggagg acgatggaca gggcgaagga gatgaggatg agcacgccgg aagcccggaa
      841 caggggatac tgttccgtga aaagggcggc gaatcccttc tttcggatct gcccgaaggc
      901 gcctttagtg ggattgttcc cgctgctgct catagcgttc gtaagccttc accacctgct
      961 ggacgatctc gtggcgcacc acgtcggaat gggtgaggta gaaaaaggcg atgccctcga
     1021 tcccgtcgag aatatccttc acctgcttga ggcccgacag ttttcccgag ggaaggtcca
     1081 cctgggtgat gtcgccggtg atgaccgcct ttgacccgaa gccgaggcgg gtaaggaaca
     1141 tcttcatctg ctccggggtg gtgttctggg cctcgtcgag aatgatgaag ctgtcgttga
     1201 gggtccgccc cctcatgtag gcaagtggtg ctatttcgat aactcccttg tccacgtaac
     1261 ggaggaaccg ctccggggag aggaggtcgt aaaaggcgtc gtaca
//
LOCUS       DAOTHD010000756         1305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_123359_length_1305_cov_2.279200, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000756 DAOTHD010000000
VERSION     DAOTHD010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123359_length_1305_cov_2.279200"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="P5219_11115"
     CDS             complement(<1..825)
                     /locus_tag="P5219_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRX27358.1"
                     /translation="MILSALLCTASGGFASAPEATPSKYPFTLDQDGIPGLGGERSVI
                     VVISDIHLGMDDRYAETQKNRAPLVDFVRKIGRSPNISELVIAGDFLDEWFIPAGVDT
                     YAGKSQKEFAQAVAANNKEVVDAFNALIREGKVKVTHVPGNHDLLIDSESMQTVFPGI
                     AEVRDVRGLGTYSPEGHPEIAIEHGHRYNFFCAPDPLSNREIAPGSILPPGYFFTRIA
                     TLSVVEGKPEPSKIRPMVTPNSLGESQDLEYLYWKIWEGLMKELPIKEDFEEKIIRT"
     gene            complement(924..>1305)
                     /locus_tag="P5219_11120"
     CDS             complement(924..>1305)
                     /locus_tag="P5219_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27359.1"
                     /translation="EWESSSLHVFAGLRYWNLKNELTIRTASAPLRHFSQSESWLDPL
                     VGIRFSTRLSPNLSFTAIVDAGGFGMGSDFTWGGMADFSWRISERTSLEIGYRYLYVD
                     YEKDGFVMDAYNDGLFIGFTWKLQ"
BASE COUNT          252 a          352 c          408 g          293 t
ORIGIN      
        1 ggtcctgatg atcttctctt cgaagtcttc ctttatgggc agctctttca tcagcccctc
       61 ccatattttc cagtagagat attcgaggtc ctggctttcg ccgagggaat tcggcgtgac
      121 catcggcctg atttttgacg gctcgggttt gccttcgacg acggagagcg tggcgattcg
      181 ggtgaagaag tatcccggag gcaggatgga gccgggggct atctcccggt ttgatagagg
      241 atcgggtgcg cagaagaagt tgtaccggtg gccgtgctcg atggcaattt ccggatgccc
      301 ttccggagag taggtcccga ggccacggac gtcacgtact tcggcgattc ccggaaaaac
      361 ggtctgcatg ctttcggagt cgatcaacag gtcgtgattc ccggggacat gggtcacctt
      421 gacctttcct tcccgtatga gggcgttgaa ggcgtctacc acttctttgt tgttggctgc
      481 gacggcctgg gcgaactctt tctggctctt cccggcgtag gtgtccacac cggcggggat
      541 gaaccattca tcaaggaagt cgccggcgat gacgagctca ctgatgttgg gagagcggcc
      601 gatcttccgg acaaagtcca cgagaggagc ccggttcttt tgagtttctg cgtaccggtc
      661 gtccattccc aggtggatgt cgctgatgac gacgatgacg gaccgctcgc cgccgagccc
      721 cggaatgccg tcctggtcga gggtgaaggg gtacttcgag ggtgtggcct ccggagcgga
      781 agcaaagccg ccggacgcgg tgcagaggag tgctgacagg atcatgatgc tcaaaatgcg
      841 ggctcttctc attgggtctc attccctttc gtgtggagtg tactggacaa catgctgcaa
      901 aaaaagacgc tcacggtgca gtcttactgc agcttccagg tgaatccgat gaacaggccg
      961 tcgttatagg cgtccatcac gaagccgtcc ttttcgtagt cgacgtagag gtagcggtac
     1021 ccgatttcga gggacgtccg ttccgagatc cgccaggaaa agtccgccat acctccccat
     1081 gtgaaatccg agcccattcc gaacccgccc gcatcgacga tggccgtgaa gctgagattc
     1141 ggcgaaagcc gggtggagaa ccggatgccc acgagggggt ccagccagga ctcgctctgg
     1201 ctgaagtgcc gaaggggagc ggacgccgtt cgtatggtca gctcattttt caggttccag
     1261 tatctcaggc cggcgaaaac gtggagcgat gacgattccc attct
//
LOCUS       DAOTHD010000757         1303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_123619_length_1303_cov_2.899840, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000757 DAOTHD010000000
VERSION     DAOTHD010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123619_length_1303_cov_2.899840"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="P5219_11125"
     CDS             <1..578
                     /locus_tag="P5219_11125"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF014347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HRX27360.1"
                     /translation="ILGTGEMGIGNTTPSTAIACAVTGVPVPMATGRGAGLTDEGLKR
                     KIAVIEGALSLHSPDPRDGIDILSKVGGFEIGGIAGAVIGAASLGIPVAVDGFISTAA
                     AVIAVLVCPACRDYMICSHKSVEIGHEHLLRFLEAEPLLDLDMRLGEGTGAALAMSLA
                     EGAAKVLNEMATFESAGVSGPVEPGSGAPGA"
     gene            585..>1303
                     /gene="cobS"
                     /locus_tag="P5219_11130"
     CDS             585..>1303
                     /gene="cobS"
                     /locus_tag="P5219_11130"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HRX27361.1"
                     /translation="MDFLRALGFLTILPCPRYESTPEDLGRSSGWFPLAGALLGVLLA
                     AAHAGLALLLPPLAAASFAAVLWAFLTRGLHLDGLADTFDGIGGGYCRKSRLAIMKDS
                     RLGTFGGIAVASALLMKVSLLAGTERTAALQALFLAPVLGRWAILFAMRFFPSARPGG
                     MGDLFRKGCTNRCLYTGTAAAVLLAAASGGMTGIMLLPLVPLFALLFSRWLASLLGGL
                     TGDSYGAVCELSEILSLCLFTV"
BASE COUNT          179 a          448 c          418 g          258 t
ORIGIN      
        1 acatcctcgg aaccggggag atgggcatcg gcaacaccac gccatcgacg gcgattgcct
       61 gcgccgtcac cggcgtcccc gtccccatgg ccactggccg gggagcgggg cttaccgacg
      121 aggggctcaa aagaaagatc gccgtcatcg agggagccct gtccctccat tcccccgatc
      181 cccgggacgg tatcgacatc ctctcgaaag tgggcgggtt cgagatcggg gggatcgccg
      241 gcgcggtcat cggtgcggcg tccctgggca ttcccgtggc agtggacggc ttcatctcca
      301 cggccgccgc ggtgatcgcc gtccttgtct gtcccgcctg ccgggactac atgatctgtt
      361 cccacaaatc ggtggagatc ggccacgagc acttgcttcg cttcctggag gcggagccgc
      421 tgctcgacct ggacatgcgt ctcggggagg ggaccggagc cgccctggcc atgtccctgg
      481 cagagggcgc cgcaaaggtg ctcaatgaaa tggccacctt cgagagcgcc ggtgtctccg
      541 ggccggtcga acccggatcg ggcgcgccgg gagcgtagaa aaccgtggat tttctccggg
      601 ctcttggctt tctgacgatt ctgccctgcc cgcggtatga gagcaccccg gaggacctcg
      661 gaaggtcctc cggatggttc cccctcgccg gggccctgct gggggtactg ttggccgccg
      721 cacacgcggg gcttgccctt cttcttcccc ccctggcggc ggcttccttc gcggcggttc
      781 tctgggcgtt tctcacccgg gggctgcacc tggacggcct ggccgacacc tttgacggca
      841 tcgggggagg gtactgccgg aaatcccggc tggccataat gaaagacagc cggctcggca
      901 ccttcggcgg aatcgctgtg gcctcagccc ttctgatgaa agtttcactc cttgccggaa
      961 cggaaaggac ggcggccctg caggcccttt tcctcgcccc cgttctgggc cggtgggcga
     1021 ttctctttgc catgcgtttc ttcccctccg cccgcccggg cggcatggga gacctgttcc
     1081 ggaaaggctg cacgaaccgc tgcctctaca ccggaacagc ggccgcagtc cttctggccg
     1141 cagcgtcggg aggcatgacc ggcatcatgc ttctgcctct tgtccccctc tttgccctgc
     1201 tcttttcccg gtggctggcc tccctcctgg ggggactcac cggcgattcc tacggggcgg
     1261 tgtgcgagct gtcggaaata ctctccctct gtcttttcac cgt
//
LOCUS       DAOTHD010000758         1302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_123753_length_1302_cov_3.241379, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000758 DAOTHD010000000
VERSION     DAOTHD010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123753_length_1302_cov_3.241379"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="P5219_11135"
     CDS             complement(<1..368)
                     /locus_tag="P5219_11135"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HRX27362.1"
                     /translation="MRYAARVGYMGTAYSGWQRQAMGTGVQEKIEESLAALEGKPVTV
                     TAAGRTDAGVHACGQVISFELEKQWRPDKLVLAANFRLPPDISVLETARVLPGFDARR
                     HALWREYRFFIWHGNALPPFL"
     gene            complement(368..1177)
                     /locus_tag="P5219_11140"
     CDS             complement(368..1177)
                     /locus_tag="P5219_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HRX27363.1"
                     /translation="MKFFNHLTFGQYVPSESPVHRLDPRCKIIAVLVLLTGVFLAKHP
                     AVFSVWGVLLACLTAAARLPFRLVLRSAKPVLILVLFTASIHIFFTSGTPLLSLGIVT
                     ISREGLVLAVYMGLRLLFLVLFASFLTLTTRPMELADGLERLFSPFARFGFPAHELAM
                     MMTIALRFIPTLLDETDRIMKAQLARGASFDRGGLWKRLKAFLPVLIPLFVIVFQRAE
                     DLAVAMEARCYRGGAGRTRMSPLAWKGTDTAALLLVPAVVILLVTIDRSLA"
BASE COUNT          275 a          352 c          468 g          207 t
ORIGIN      
        1 agaaagggag gaagggcgtt tccgtgccag atgaagaacc ggtattctcg ccagagggcg
       61 tggcgccggg cgtcgaaccc cggcaggacc ctggccgtct cgaggacgga tatgtcgggg
      121 ggcaggcgga agttggccgc cagaacgagc ttgtcggggc gccactgctt ctccagttcg
      181 aaggagatca cctgcccgca ggcatggacc ccggcgtcgg tccggcctgc ggcggtgacg
      241 gtgacgggtt ttccctcgag ggcggccagg ctttcctcta ttttttcctg gacgcccgtg
      301 cccatggcct gccgctgcca tccggaatag gccgttccca tgtagccgac ccgggctgcg
      361 tatcgcatca ggcgaggctc cggtcgatgg tgacgagaag aatgaccacg gcggggacca
      421 ggagaagggc cgccgtatcg gtccccttcc atgcgagggg ggacatgcgg gtcctgcccg
      481 ctccgccccg gtaacaccgg gcttccatgg ccacggcgag gtcttccgcc cgctggaaga
      541 cgatgacgaa gagcggtatg agcaccggga gaaaggcctt cagccgcttc cacaggccgc
      601 cccggtcaaa ggaggcgccc cgggcgagct gggctttcat gatgcggtcc gtttcgtcaa
      661 gaagggtcgg gatgaaccgg agagcgatgg tcatcatcat ggccagttca tgggcgggaa
      721 agccgaaccg ggcgaagggg gaaaagagcc gttccagccc gtcggccagt tccatgggcc
      781 tggtggtcag ggtgagaaag gaggcgaaca gtacaaggaa caggaggcgg agtcccatgt
      841 agactgcaag tacaagtccc tcccgggata tggtgacgat gccgagggag agaagaggtg
      901 tcccagaagt aaagaaaata tgaatcgagg cggtgaaaag caccaggata aggacgggtt
      961 tcgccgaccg caggacgagc ctgaagggaa gccgggcggc ggcggtcagg cacgcgagaa
     1021 ggacacccca tacagaaaag acggcgggat gtttcgcgag aaaaacgccg gtcagcagga
     1081 cgaggacggc aatgattttg caccgggggt cgagtcggtg caccggtgat tccgagggaa
     1141 cgtactggcc gaaggtaaga tggttgaaga atttcatttc tgactcccga agagggcgga
     1201 tttgatttct ttccagtccc aggtgagggg aacatctttt ccccgttccc ggagggcggc
     1261 ggacagggcg gcaatctcgg gaagctgcag tcccggcacg gg
//
LOCUS       DAOTHD010000759         1300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_124074_length_1300_cov_2.539759, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000759 DAOTHD010000000
VERSION     DAOTHD010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124074_length_1300_cov_2.539759"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..866
                     /gene="sdaAB"
                     /locus_tag="P5219_11145"
     CDS             183..866
                     /gene="sdaAB"
                     /locus_tag="P5219_11145"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HRX27364.1"
                     /translation="MGFLDIIGPVMIGPSSSHTAGAARLGKLAGACWGDDPVREVALF
                     LRGSFAFTSKGHGTDKALVAGLLGMEPDDEGIRTALATAAERGFPFRFEREDVDGAHP
                     NSVRFLFSGRDGRTMEIVGASVGGGAVELQEIDGFSVKVSGDLPTLVTFHRDVHGVVA
                     AVAALLADRKINIASMTLHRKTRGGMASLVAEMEMEGNPDPALPGDVEKVHPAIVRVV
                     PLYGRGGPS"
     gene            863..>1300
                     /locus_tag="P5219_11150"
     CDS             863..>1300
                     /locus_tag="P5219_11150"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HRX27365.1"
                     /translation="MRSIADIVEQCRLSGRSFSEQALAMESEETGVAESLLRTGMLSR
                     LKDMEFSVREAVSKEWRGLIVPAEAHLIEQYAGQGGAFSGPYVLSAARIAMAVSTYNA
                     AMGRIVAAPTAGSCGILPGMLFACREQFGTEDDLLLSGLFAAAA"
BASE COUNT          228 a          385 c          440 g          247 t
ORIGIN      
        1 acatggagtt cgaggccatg tatatctgtc ccgtgtgcgg acacaccgtt gccgacgaag
       61 ccccggacaa atgcccggtg tgcggcgtgg cgaaggagaa atacgtccgc ttctaagtgt
      121 cggatgaggg gaagggacga ccgtcccttc ccctcatttc tttttttcgg ggaggaatcc
      181 gcatgggctt tctggacatc atcgggccgg taatgatcgg tccgtcgtca agccacacgg
      241 ccggcgccgc ccggctggga aagctggccg gggcgtgctg gggggacgat cctgtcaggg
      301 aagttgccct tttcctgagg ggaagtttcg ccttcacgtc caaaggacac ggaacggaca
      361 aggccctggt tgccgggctt ctcggcatgg agcccgacga cgagggcatc cggacggctc
      421 tcgcaacggc cgctgagcgg ggctttccct tccgtttcga gcgggaggac gtggacggcg
      481 cccatcccaa ttcggtccgg ttcctctttt ccggcaggga cggaagaacc atggaaattg
      541 tgggggcctc cgtgggggga ggagcggtgg aactccagga gatcgacggc ttttcggtga
      601 aggtcagcgg cgacctgcct acccttgtga ccttccacag ggacgtccac ggagtggtgg
      661 ccgcggtggc cgcccttctt gcagaccgga agatcaacat cgcctccatg acccttcaca
      721 gaaagacccg gggaggaatg gcctccctcg tggcggagat ggagatggaa ggaaatcccg
      781 acccggctct tcccggcgac gtggaaaagg tccatcctgc catcgttcgg gtggttcccc
      841 tctacgggag aggaggtccg tcatgagatc catcgctgac atcgtggagc agtgccggct
      901 gtcaggacgg tctttttccg aacaggccct ggcaatggag tcggaggaaa ccggagtcgc
      961 cgaaagtctc ctccggacag gaatgctctc caggctgaag gatatggagt tctcggtcag
     1021 ggaggccgtg tcgaaagagt ggcggggcct cattgttccg gcggaggcac accttattga
     1081 acagtacgcc ggacaggggg gcgccttctc gggaccctac gtcctttccg ccgcccggat
     1141 cgccatggcg gtttccacct acaacgccgc catggggcgc atcgtggcgg cacccacggc
     1201 ggggagctgc ggcatcctgc ccggcatgct cttcgcgtgc cgggaacagt tcggcacgga
     1261 ggacgacctt cttctttcgg gcctgttcgc cgccgcggct
//
LOCUS       DAOTHD010000760         1299 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_124206_length_1299_cov_2.835209, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000760 DAOTHD010000000
VERSION     DAOTHD010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124206_length_1299_cov_2.835209"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="P5219_11155"
     CDS             complement(<1..680)
                     /locus_tag="P5219_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRX27366.1"
                     /translation="MKKLSKILAFLSIAALFAAGAAFAAPMQLTMGTGGTAGTYYPLG
                     GALSQIISDNSGGTVNITAQSTGASIENINLLAAGDVDLIIVQNDMSDYAYNGTEFFK
                     DRKIENITAIARLYPEHIQVIASKDSGIKSIADFKGKNISVGAPGSGNEANVRQILDA
                     YGITYNDFKAHFISYAETTDHFKDRLVDAFSYTTGAPNPSIQDISTLQDLVFVPIDGE
                     IRDKLIAK"
     gene            complement(830..>1299)
                     /locus_tag="P5219_11160"
     CDS             complement(830..>1299)
                     /locus_tag="P5219_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRX27367.1"
                     /translation="PDVDTVVVPLGGGGLLAGVAAAIKETRPSVRVVAVEPAGIQRYA
                     ESRKAGKPVEVPMGATLADGLMITMTGEHNYPLIDKYVDEIVPASDEFIYKALMEIVF
                     KSKLLVEPSASVGVAAALEGSFKVKPGEKICFFLSGGNIDPDKLASFIAHETA"
BASE COUNT          242 a          336 c          424 g          297 t
ORIGIN      
        1 tacttcgcga tgagcttgtc ccttatctcg ccgtcgatgg gaacgaagac gaggtcctga
       61 agggtgctga tgtcctggat cgaggggttg ggagcgccgg tggtgtagga aaaggcgtcc
      121 acgaggcggt ccttgaagtg gtcggttgtc tcggcgtagg aaatgaaatg ggccttgaag
      181 tcattatagg tgataccgta cgcgtcaaga atctggcgga cgttggcttc gtttccgctt
      241 cccggagcgc ccacggagat attctttccc ttgaagtcgg cgatggactt gatgccgctg
      301 tccttggagg cgatgacctg gatgtgctcg ggatagagcc tggcgatggc ggtgatgttt
      361 tcgatcttcc ggtccttgaa gaactcggtc ccgttgtagg cgtagtcgga catgtcgttc
      421 tggacgatga tgaggtccac gtcccctgcg gcaagaaggt tgatgttttc gatggaggct
      481 cctgtggact gggcggtaat gttgacggta ccgcctgaat tatcgctgat gatctgggaa
      541 agggctccgc cgaggggata ataggttcca gccgttccgc ctgttcccat ggtgagctgc
      601 atgggcgccg caaaggccgc gcctgcggcg aaaagggccg cgatggaaag aaacgccagt
      661 atcttggaca actttttcat gaaattccct ccttcagata gtgttttccg gtgcatatac
      721 atcgtaattt gttttgggga ttccgtcaac cggatatatc agacatggcg tacagcacgg
      781 gacagaaaaa agcccgggaa atcccgggct ttttttgtcc ttttcagtct caggccgttt
      841 cgtgggctat gaacgacgcg agcttgtcag ggtcgatgtt gccgccggac aggaagaagc
      901 agatcttttc gcccggcttc accttgaagc tgccttcgag ggccgccgcc acgcccaccg
      961 atgcggaggg ttccacgagc agcttgctct tgaagacgat ctccatgaga gccttgtaaa
     1021 tgaactcgtc ggaggcggga acgatctcgt ccacgtactt gtcgatgagg ggatagttgt
     1081 gttcgccggt catggtgatc ataagaccgt cggcgagggt ggcgcccatg gggacctcca
     1141 cgggcttccc ggccttccgg ctctcggcgt agcgctggat cccggcgggt tccacggcga
     1201 ccaccctcac ggagggccgg gtctccttga tggccgcggc tacgccggcg aggaggccgc
     1261 cgccgccgag gggaaccaca acggtgtcca cgtcgggga
//
LOCUS       DAOTHD010000761         1299 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_124252_length_1299_cov_2.140675, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000761 DAOTHD010000000
VERSION     DAOTHD010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124252_length_1299_cov_2.140675"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="P5219_11165"
     CDS             <1..875
                     /locus_tag="P5219_11165"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HRX27368.1"
                     /translation="GKPDEAMIISASRRTDIPAFYASWLERRLEDGFVLVRNPRNPAQ
                     VRRISLVPADVDCLVFWTKDARPLFGLLDRLDGAGYPYYFQWTVTPYGSGLEPGIAPK
                     EEILAAFRALAGRLGSHRVVWRYDPVIVSEEYPAERHREEFSRMCRFLEGSTERCVLS
                     FVDIYRKNRGRGFRETSPEIMHGLVRSFAGSAAGRGISLHTCCEAEDFGGEGIPRGAC
                     VDRTLAERLAGHPLDAGKDRNQRPLCGCAASADIGAYDTCPAGCLYCYAVRDLRTAEA
                     NRARHDPDSPFLVP"
     gene            complement(920..>1299)
                     /locus_tag="P5219_11170"
     CDS             complement(920..>1299)
                     /locus_tag="P5219_11170"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRX27369.1"
                     /translation="DGEIGAPVSRYVDTLIAMNQPSLEKFLGSVRPGGIILLNSSLCR
                     WEGKRTDVRVAEIQADAIALEIGSPKVANIVVIGAYIQLSGALSEQEMLDAIEEKLGK
                     RPELLEMNRKALRTGMERVTLKE"
BASE COUNT          227 a          425 c          414 g          233 t
ORIGIN      
        1 ccggtaaacc ggatgaagcc atgatcatca gtgccagcag gcggacggac atccccgctt
       61 tttacgcctc gtggctggag cgccgcctgg aggacggctt cgtcctcgtg cggaaccccc
      121 ggaacccggc ccaggtgagg agaatctccc tcgtccccgc cgacgtggac tgcctcgtct
      181 tctggacgaa ggacgcccgt cctctgttcg gcctcctgga caggctcgac ggggcagggt
      241 atccctatta tttccagtgg accgtcaccc cgtacggttc cggcctggag ccggggatcg
      301 ccccgaaaga ggagattctc gccgctttcc gggccctggc aggaaggctc gggtcccata
      361 gggtcgtctg gcggtacgac ccggtgattg tctcggagga atatcctgcg gaacggcaca
      421 gggaggagtt ctcccggatg tgccgctttc tggaaggcag cacggaacgg tgcgtcctga
      481 gttttgtgga catctacaga aagaaccggg gcaggggctt ccgggaaacc tcgccggaga
      541 tcatgcacgg cctggtccgt tcctttgccg gaagcgccgc cggacggggg atttcccttc
      601 acacctgctg cgaggcggaa gatttcggcg gggagggcat cccccgggga gcctgcgtcg
      661 acaggaccct ggcggaacgg ctcgccggac accccctgga tgccggaaag gacaggaacc
      721 agcgccccct gtgcgggtgt gccgcaagcg ccgacatcgg cgcatacgac acctgtcctg
      781 cggggtgcct ttactgctac gccgtccggg acctccggac agccgaggcg aaccgggccc
      841 ggcacgaccc ggactccccc tttctcgtcc cgtaaaaaaa gcggccccgg aagggccgct
      901 ccagcacaaa ctgacggaat tattccttca gggtcacccg ctccattcct gtccgaagag
      961 ctttcctgtt catttcgagc agttcgggcc gcttgccgag tttttcttcg atggcgtcca
     1021 gcatctcctg ttccgaaagg gccccgctca gctgaatgta ggcgccgatg accacgatgt
     1081 tggccacctt gggcgacccg atctcgaggg cgatggcatc ggcctgaatc tccgcaaccc
     1141 ggacgtccgt ccgcttcccc tcccagcggc agagggagct gttgagcagg atgattcccc
     1201 cgggccggac agagccgaga aacttctcca gggacggctg gttcatggcg atgagggtat
     1261 ccacataccg agacacggga gcaccgatct cgccgtcgg
//
LOCUS       DAOTHD010000762         1298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_124367_length_1298_cov_2.497184, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000762 DAOTHD010000000
VERSION     DAOTHD010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124367_length_1298_cov_2.497184"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="P5219_11175"
     CDS             <1..797
                     /locus_tag="P5219_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRX27370.1"
                     /translation="LSADDLAAAASVEAFSNHPLAKAIASASDERRPVGGVEEITGEG
                     VRGMVDGVEYFVGKPGDPARYDEHLSMGRTVVEVRRAGVLAGFLAVEDPLREDAVEGV
                     KRLSAMGITCVMATGDNEKTARAVAARVGIVEVHAGVRPDGKLDLVRQLQARGGKVLM
                     TGDGMNDAAALKGADIGVAVGSGTDLAIDSADIVIVKGGISRLADAVAISRKTFTVIR
                     QNLFWAFAYNVVAIPLAMAALLHPVIAEGAMAFSSISVILNSMRVK"
     gene            complement(868..>1298)
                     /gene="kduI"
                     /locus_tag="P5219_11180"
     CDS             complement(868..>1298)
                     /gene="kduI"
                     /locus_tag="P5219_11180"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HRX27371.1"
                     /translation="CSPNSPERFFIFQGADLGPEKANVRTINQYIHPEVCSTCQLMMG
                     LTQLENGSVWNTMPCHTHERRMEVYFYFDIPRDGVTFHIFGRPEETRHIVVRNEEAVI
                     NPSWSIHSAAGTSCYSFIWSMVGENQEFTDMDHVPMSVMK"
BASE COUNT          237 a          388 c          426 g          247 t
ORIGIN      
        1 acctttcggc ggacgacctg gcggctgcgg catccgtgga ggccttctcc aaccatcccc
       61 tggccaaggc catcgcatcg gcttccgatg agcgtcgccc cgttgggggc gttgaggaga
      121 tcaccggcga aggtgtccgg ggaatggtgg acggggtgga atacttcgtc gggaagcccg
      181 gagacccggc acggtacgac gaacatctct ccatgggccg caccgtggtg gaagtccggc
      241 gggcaggagt gctcgcaggc tttctcgccg tggaggaccc cctccgggag gacgcggtgg
      301 agggcgtgaa gcgcctgtcc gccatgggca tcacctgcgt catggccacg ggggacaacg
      361 aaaagaccgc ccgggcggtg gctgcacggg ttgggatcgt cgaggtccac gccggggtcc
      421 ggcccgacgg aaagctcgac ctggtgcgtc agctccaggc ccgggggggc aaggtcctca
      481 tgaccggcga cggcatgaac gacgcagcgg ccctcaaggg cgccgacatt ggggtggccg
      541 tggggtcggg caccgatctc gccatcgaca gcgccgatat cgtcatagtc aaaggaggca
      601 tctcccggct ggccgacgcc gtggccattt cccggaagac cttcaccgtg atccggcaga
      661 acctcttctg ggccttcgcc tacaacgtgg tggccattcc cctggccatg gcggccctgc
      721 tccatcccgt catcgccgag ggggccatgg ccttcagctc catctcggtg atcctgaact
      781 ccatgcgggt gaagtgagcc ggaaaactcc gaaaacgaag aagcccggaa gtccccaggg
      841 gagggatttc cgggcttctt gtgccggcta cttcatgacc gacatgggta catggtccat
      901 gtcggtgaat tcctggttct cccccaccat gctccagatg aagctgtagc atgatgtgcc
      961 tgccgcgctg tggatggacc agctcgggtt gatgacggcc tcttcatttc ggacgacgat
     1021 atggcgggtt tcttcaggcc tgccgaagat gtggaacgtc acgccgtccc tggggatgtc
     1081 gaaatagaaa tagacttcca tccgccgttc atgggtatgg cacggcatgg tgttccagac
     1141 gctgccgttt tcaagctggg tgagacccat catgagctgg caggtactgc agacctccgg
     1201 gtggatatac tggttgatgg tgcggacatt ggctttttcc ggccctaaat ccgcaccctg
     1261 aaaaataaaa aacctctctg gactgttcgg agaacagt
//
LOCUS       DAOTHD010000763         1295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_124819_length_1295_cov_2.136290, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000763 DAOTHD010000000
VERSION     DAOTHD010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124819_length_1295_cov_2.136290"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="P5219_11185"
     CDS             <1..268
                     /locus_tag="P5219_11185"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709133.1"
                     /note="catalyzes the formation of D-ribulose 5-phosphate
                     from 6-phospho-D-gluconate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HRX27372.1"
                     /translation="DLMKDLLPSWRDVAATAIRHGIPIPSMSSGLAYFDGYTSEFLPA
                     NLLQAQRDYFGAHMYQRLDAERGRFFHTNWTERGGNTVSGTYVV"
     gene            320..1261
                     /locus_tag="P5219_11190"
     CDS             320..1261
                     /locus_tag="P5219_11190"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRX27373.1"
                     /translation="MPLSAGCAPFGEVPVLKILRTYHPYAFTAIFCWSLAYVFTRLAL
                     GHFSPLSLGFLRCAIASFILGAVGFRTGMKLPRREDVKYFAAAGASGFFLYLWAFNQG
                     ILTVTSATSSIIISSAPVITALLAVLFLGERVGRLQWAAIAVEFAGILILSLMKGAFS
                     VNRGILWLLLAALVLSVYNLVQKGLTGRYSGLQTATFSITAGAAMLAVFAPRAFRELA
                     AAPPAQLFNLAMLSVFSSAIAYVAWSKAFEKTRDTASVSNYMFLTPLISSLLGFLIAG
                     ERPDGATLIGGAVILYGLALFNLGKRQNIGPIQTEKE"
BASE COUNT          214 a          457 c          361 g          263 t
ORIGIN      
        1 ggatctcatg aaggatctcc ttccctcctg gcgggacgtg gccgcgaccg ccatcaggca
       61 cggcatcccc ataccttcca tgagctcggg cctggcctac ttcgacggct acaccagcga
      121 atttctgccc gcgaacctgc tgcaggccca gcgggactac ttcggcgccc acatgtacca
      181 gaggctggac gccgaaaggg gcaggttctt ccacaccaac tggacggagc ggggaggcaa
      241 caccgtctca ggcacctatg tggtctgatt ttcccgtctc cggagccggg aacggaaaac
      301 acgacaccct gcggaaggca tgccgctttc cgcggggtgt gctcccttcg gggaggtgcc
      361 ggtcctgaaa attctccgga cctatcaccc ctacgccttc acggccattt tctgctggtc
      421 tctggcctat gtcttcaccc gcctggccct gggacacttt tcgcccctct ccctcggctt
      481 tctgcggtgc gccatcgcct ccttcatcct cggtgcggtg ggcttccgca caggaatgaa
      541 gctcccccgg agggaagacg tcaagtactt cgcggcggcc ggggcctccg gcttctttct
      601 ttacctgtgg gccttcaacc agggaatcct caccgtcacc tcggccacga gcagtatcat
      661 catttcctcc gctccggtca tcaccgccct ccttgccgtg ctcttcctcg gcgaaagggt
      721 gggaaggctc cagtgggcgg ccattgccgt cgagtttgcc ggaatcctca tcctgtccct
      781 catgaagggg gccttctccg tgaacagggg tatcctctgg cttctcctcg ccgccctggt
      841 gctcagcgtc tacaacctgg tccagaaggg cctcacgggc agatattccg gtctccagac
      901 cgccaccttc agcatcacgg cgggggcggc catgctcgcc gtctttgcgc cccgggcgtt
      961 ccgggagctg gcggcggcgc cgccggccca gctcttcaac ctcgccatgc tttccgtctt
     1021 ctccagtgcc atcgcctacg tagcctggtc gaaggctttc gagaaaacca gggacacagc
     1081 ctcggtgagc aactacatgt tcctgacgcc tctcatctcc agcctgctcg gcttcctcat
     1141 cgcgggggaa cggcccgatg gcgccactct catcggcggg gcggtcatcc tctacggcct
     1201 cgccctcttc aacctgggca agcggcagaa catcggaccg attcagacag aaaaagaata
     1261 aaaaaagggg acggcacccg gccgtcccct ggata
//
LOCUS       DAOTHD010000764         1294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_124883_length_1294_cov_3.335755, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000764 DAOTHD010000000
VERSION     DAOTHD010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124883_length_1294_cov_3.335755"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="P5219_11195"
     CDS             complement(<1..1167)
                     /locus_tag="P5219_11195"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRX27374.1"
                     /translation="MLRQRVRSFAAALCLLALIVLPAAGADVMDLYSGSSRAGQVASE
                     KRGSGIMVSARDMAGVLGLETSEKGDTLIVTAGRSKLQLVAGAAAAWLDAELVPLAAS
                     TVQEGKEWLVESRSALKVFEGLLLRSGKPGNLRWEGTPHAGTPSSSPSSPSPQPPEAR
                     VSAPQPSLPAGPVISAVRWGSDDDKIRAVLDYEGPNAPEIQQGAGSVKVAFLLGKSGV
                     PDLNSPHGEVKVSSVNFGDRVVIEFSSSLPLKEIIPLEGPPRIVIDFSRTGTPVSVKN
                     PKPSKTPPAIQTVPPAVQVRDPNRKKGPKVVVIDPGHGGKDPGAVANGIREKDVNLSI
                     SRLLAQKLKKAGYDARLTRDKDIYLTLQQRTDLANKWNADVFVSIHANALPPGKH"
BASE COUNT          261 a          366 c          396 g          271 t
ORIGIN      
        1 atgtttgccc ggtggaagag catttgcatg gatactgacg aaaacgtcgg cattccattt
       61 gttggccagg tcagttcttt gctggagggt gaggtagata tccttgtccc tggtaagccg
      121 ggcgtcgtat ccggcctttt tcagcttctg ggcgagaagg cgggaaatgg agagattaac
      181 gtccttttca cggatgccgt tggctacggc ccccggatct tttccgccat gccccggatc
      241 gatgacgacg actttcggtc cctttttcct gtttgggtcc ctgacctgaa ccgcaggggg
      301 aaccgtttgt atagcgggtg gcgtttttga gggcttcggg tttttcaccg aaacaggagt
      361 ccccgtcctg gaaaagtcga tgacgattct cggaggacct tccaggggaa tgatctcctt
      421 caggggaagg gaagaggaga actcaataac cacacggtca ccgaagttga ccgaggacac
      481 cttcacttcg ccgtggggag aattgaggtc cgggactccg gatttcccga ggagaaaggc
      541 aaccttcact gaaccggcgc cctgctggat ttcgggtgcg ttcggacctt cgtagtccag
      601 gacggcccgt attttgtcgt cgtcgcttcc ccaccggaca gcagagataa cgggccccgc
      661 tggcagggaa ggctgcgggg cggaaacccg ggcttccggt ggctggggac tgggggagga
      721 aggagatgaa gaaggtgttc ccgcatgggg agttccctcc catcggaggt tccccggttt
      781 tcccgaacgc aggaggagcc cctcaaagac cttgagggcc gaacggcttt ccacgagcca
      841 ttccttcccc tcctggacgg tggaggcggc cagcggaaca agttcggcat ccagccatgc
      901 cgccgcagcg ccggctacaa gctggagctt gctccgcccg gcggttacga tgagagtgtc
      961 tcctttttcc gaggtttcaa gaccgagaac gccggccata tcacgggcgg ataccatgat
     1021 gccggaacct cttttttccg aggccacctg tccggccctg gaggaacctg agtaaagatc
     1081 catcacgtcc gcccctgccg ccgggaggac gatcaatgcc aggaggcaga gggctgcggc
     1141 gaaactacga acccgctgcc gcaacatccc gcaccaccag ggaacaggcc ccggtactcc
     1201 cgtaaaaacg aaaatcgttg ccgatgaggt caatggtgtt cagtatatct ttaaggtttc
     1261 cggcaatggt cattcctgaa actgcacctc ccag
//
LOCUS       DAOTHD010000765         1293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_125037_length_1293_cov_3.224556, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000765 DAOTHD010000000
VERSION     DAOTHD010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125037_length_1293_cov_3.224556"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1243)
                     /locus_tag="P5219_11200"
     CDS             complement(116..1243)
                     /locus_tag="P5219_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRX27375.1"
                     /translation="MALVFLVCLFAVPACAADPIRIGEIATVTGDFAAYGVAEVESVK
                     IAVAEINAAGGILGRPVEVVMYDCRTRQEDMVNAARRLVEQDKVSVVIGPSGSGLCIA
                     AAPVFNRGKVPHIGTLPTNPLVTVDEKGQVRPYNFRICFLDPYQGKMIAAFAAKDLGK
                     KKAAVLYDVSSDYSQGLREFFMKSFKEYGGEIVADEGHRAEDVDFRAQLTVIKQANPD
                     VLVIPTMGKCLPLAVKQARELDLNIEIVGGDGYGDFMWEIAGDAMKDTYWVSHVDKAD
                     PALAGFFKKYEEQTGTECMEFMNAVMAYDSVYWVKDAMERAGSDDPVKVRDALEATKD
                     LKLMHATLTMDEFHNPKDKDGIMLVSDIAGKKAVFFKKIKP"
BASE COUNT          219 a          420 c          389 g          265 t
ORIGIN      
        1 gggttcctgg cggaggacat cgccgcggag ctgggcggac ctgatgcctg atacaggaat
       61 ataaaagagg gcgggggatt tcccccgccc tctgcttatc agccgccctg gctcgttacg
      121 gctttatctt cttgaagaag accgcctttt tgccggcgat gtcggacacg agcatgatgc
      181 cgtccttgtc cttggggttg tggaactcgt ccatggtcag ggtggcgtgc atgagcttca
      241 ggtccttcgt ggcctccagg gcgtcccgta ccttcacggg atcgtcgctg ccggcccgct
      301 ccatggcgtc cttcacccag tagacgctgt cataggccat gacggcgttc atgaactcca
      361 tgcactcggt gccggtctgc tcctcgtact tcttgaagaa accggcgagg gccgggtccg
      421 ccttgtccac gtggctcacc cagtaggtat ccttcatggc gtcgccggcg atctcccaca
      481 tgaagtcgcc gtagccgtcg ccgcccacga tctcaatgtt caggtcaagc tcccgggcct
      541 gcttcaccgc cagggggagg cacttgccca tggtggggat gaccagcacg tcggggttgg
      601 cctgcttgat caccgtgagc tgggcgcgga agtccacgtc ctcggccctg tggccttcgt
      661 cggccacgat ctctccgccg tattccttga aggacttcat gaagaactcc cggagcccct
      721 gggagtagtc gctggacacg tcatagagga cggccgcctt tttcttcccg aggtccttgg
      781 ccgcaaaagc ggcaatcatc ttgccctgat aggggtcaag gaagcagatg cggaagttgt
      841 agggacggac ctgccccttc tcgtccacgg tcaccagggg attcgtgggg agggttccga
      901 tgtggggcac cttgccgcgg ttgaacacag gggcggcggc gatgcagagt ccgcttccgc
      961 tgggcccgat gaccacgctg accttgtcct gctccacaag gcgccgggcc gcgttgacca
     1021 tgtcctcctg gcgggtgcgg cagtcgtaca tgacgacttc cacgggcctg ccgaggatac
     1081 cgccggcggc gttgatctcc gccacggcga tcttcaccga ttccacttcc gccacgccgt
     1141 aggcggcaaa gtcacccgtc accgtcgcga tttcaccgat ccggatagga tccgccgcac
     1201 aggcgggcac ggcaaaaaga cacaccagga acaccaacgc catcgctttc cgaatgctgc
     1261 cgaaactcct cattcttcca acctcctcac atg
//
LOCUS       DAOTHD010000766         1293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_125092_length_1293_cov_2.159128, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000766 DAOTHD010000000
VERSION     DAOTHD010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125092_length_1293_cov_2.159128"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            793..>1293
                     /locus_tag="P5219_11205"
     CDS             793..>1293
                     /locus_tag="P5219_11205"
                     /inference="COORDINATES: protein motif:HMM:NF014281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HRX27376.1"
                     /translation="MLKLQFLRNVSIRVRLFVLTAVLCLFTAGASFVGYQRLVAAERD
                     MADMFRSGVLPVQWMNDSRANMQAIRGNLNAMMLTRDETQNRALLEDIVNRRKSNDEN
                     LENYGKIELEEFEIKRLAEAKKHLMEFRKATEEVIRLAQNNQNQEAYEAFLQKAQKPM
                     NDVMDAL"
BASE COUNT          319 a          366 c          290 g          318 t
ORIGIN      
        1 cccctggcct ccgcatcggc aatgtcgtcc tcgtcgaagc cggggacatg atcctcgtcg
       61 ccggtcacag ggaagacctg acgaagtggg ttgaggaata ataatcgccc tcccggcagc
      121 ccgggaagcc cctgattgtt tgagatcgtt ccaggtttcg gctgatgcct gtcattcaga
      181 gaggactgca tgttcagaaa gtccgccttt cctcaccggg cggctgtctg ccttgcttcg
      241 acagtccctt gcccattcgg caaagtaacg gtcattcaaa cacccacaac cttttcgtcg
      301 cagtttccat tcccttcctc aaaaaacaca ccatttcatc cgttttccat gaaaattatc
      361 cccctcacgc agggactata ttcccactat ctttagtcgt tcggctctcg ataagtattc
      421 tcaataacta tttcatatgc ttcctaagaa tcaatcaaaa tcaaaagcat tttaacgttt
      481 tttcaaaaaa ggcgccggtt atcccccctt tttccacccc gcccgccgtt gacatctcca
      541 tttactgatg ggatgatgct tgaaaacatt tttcgaagaa attttcacca tcctcgaaaa
      601 ggtttcatgc aaccccctgt gaggcatcct gttcatttcc ggattttcct catccagata
      661 ctgagcgaaa gccgttcgtt caaaaaaaca ttttcaaccg ttttcaggct cataccagtg
      721 gtgcgtgggg cactggatct gacgcaggca gccccttcgg gctgcctatt ttttcgtggg
      781 gagtgatttt ttttgctgaa gctgcagttt ctcagaaatg tttccatccg tgtccgcctt
      841 ttcgtcctga cagccgtatt gtgtcttttc accgcaggcg cctcgtttgt aggctaccag
      901 cgccttgtcg ccgccgaaag ggacatggcg gacatgttcc ggagcggtgt attgcccgtc
      961 cagtggatga acgattccag ggccaacatg caggcaatcc ggggaaatct caacgccatg
     1021 atgctgaccc gtgacgagac acaaaaccgc gcccttctcg aagacatcgt caaccggaga
     1081 aagagcaacg acgaaaacct ggaaaactac ggcaaaatcg aactcgaaga atttgagatc
     1141 aagcgccttg ccgaagcaaa aaagcacctc atggaattcc ggaaagccac ggaagaggtt
     1201 attcggctgg cacaaaacaa ccagaaccag gaagcctacg aggcgtttct gcaaaaagcc
     1261 cagaagccca tgaacgacgt catggatgct ctc
//
LOCUS       DAOTHD010000767         1291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_125349_length_1291_cov_2.631877, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000767 DAOTHD010000000
VERSION     DAOTHD010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125349_length_1291_cov_2.631877"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="P5219_11210"
     CDS             complement(<1..1266)
                     /locus_tag="P5219_11210"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HRX27377.1"
                     /translation="MGTGWGVSLPRGVPFCAVMIIALCFFAPAAQGKSASGPGGGDGL
                     YVVTADVVPMFEKPAAKVPRVTDLWELPDFYGIVVYGNHVRLRAVTDSKLRQFAGGWY
                     ALLSPEDGTVLSYIQKKGIEKVPEYTSYKPRSFMVKKDGAELRLQPGKGGSAGHLSIE
                     GFSLVRGETVTAVGEYSEGKENWLLLEFSTDTRFGSGGVGSRYAWGKKADFGSLEGYG
                     PDNARVDKGLLPSKMRYSAMSSFYGDGFRGMDAPEKLTEFLPVTKDMVASLLRKGFYI
                     DDPWAMDEYALQVDDLADYYRYTTDYQADFITTDLFLHAFHLVFDRMLQKFERTYLAP
                     ALGKNMKTALSALSGVEGACASAGAGETWNKARDLFAIPLALLEEKPGTRTKLSKNAS
                     EEVRRILAAKDVTDSAVTGAKMDYTSFRPR"
BASE COUNT          247 a          412 c          371 g          261 t
ORIGIN      
        1 cctgggcctg aaggatgtgt agtccatttt cgccccggtg acggcggagt ccgtcacatc
       61 cttcgcggcg aggatccggc gcacttcttc ggaagcgttc ttcgacagct ttgtccgtgt
      121 tcccggcttt tcctccagga gagccagagg gatggcgaac aggtcccggg ctttattcca
      181 ggtctccccc gctcccgccg aggcgcaggc gccctcgacg ccggagaggg cggaaagggc
      241 cgtcttcatg tttttcccga gggcaggggc aaggtaggta cgctcgaatt tctgcagcat
      301 ccggtcgaat accaggtgga aggcgtggag gaacaggtcc gtggtgatga agtctgcctg
      361 gtaatcggtg gtgtatctgt agtaatccgc caggtcgtcc acctggaggg catattcgtc
      421 catggcccac gggtcgtcga tgtaaaagcc cttcctcagg agggaagcaa ccatgtcctt
      481 cgtcacggga aggaattccg tcaatttttc cggggcgtcc attccccgga agccgtcacc
      541 gtagaaagag gacatggcgg agtaccgcat cttcgacggc agaagccctt tgtcaaccct
      601 ggcattgtcg ggcccatagc cttccaggga gccgaaatcg gcctttttgc cccaggcgta
      661 ccgggagccc acgccgccgc tgccgaacct ggtgtcggtt gaaaactcca agagcagcca
      721 gttttccttt ccttccgaat attcgcccac cgccgtcacc gtctcacccc ggacaaggga
      781 aaacccctcg atggagaggt ggccggccga tccgcctttc ccgggctgca gccgcaattc
      841 agcaccgtcc ttcttcacca tgaaggaacg gggcttgtag cttgtgtact cagggacttt
      901 ttcgatgccc ttcttctgga tgtaagagag caccgtcccg tcttccggcg agagaagggc
      961 gtaccatccg ccggcgaact gcctgagctt gctgtccgtt acggcgcgca gcctcacgtg
     1021 attgccgtag acgacgatcc cgtagaaatc cggaagctcc caaaggtccg tcacccgggg
     1081 aactttcgcc gcgggttttt cgaacatggg caccacatcc gccgtgacga cgtagagccc
     1141 gtccccgccc ccggggccgg aagcactctt cccctgcgcc gccggggcaa aaaagcacaa
     1201 cgcaatgatc atgacggcac aaaacggaac tcccctcggc aacgaaacgc cccacccggt
     1261 tcccatggct tttcttccat tcatcccgat a
//
LOCUS       DAOTHD010000768         1289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_125705_length_1289_cov_1.776337, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000768 DAOTHD010000000
VERSION     DAOTHD010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125705_length_1289_cov_1.776337"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /gene="dctP"
                     /locus_tag="P5219_11215"
     CDS             <1..834
                     /gene="dctP"
                     /locus_tag="P5219_11215"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRX27378.1"
                     /translation="KGKFAITIYPRDELSGGNQAAGIELLQIGATDIHLQDALVWSSI
                     AKKSIIPCFPWLLPTNAEVDKYVMNGEGGKALRQIISDAGCVCLAVGENGYRQVANNR
                     NPIKMPEDMKGLKIRVPGSNVHVNLLKYIGADPLTMNQSEVYTSLQQGAIDACENTLD
                     LLFTQNTLEVVSHLTLWNYSYDPLFLSVSNKLWNSLSDEEKVIFQECAEEAMKHQVKA
                     ARETVESLMKNFGKYPKVQVVTSLTEDQVAAFKKAVAPIYEDYRKTFGEDLFAKFGYT
                     F"
     gene            996..>1289
                     /locus_tag="P5219_11220"
     CDS             996..>1289
                     /locus_tag="P5219_11220"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRX27379.1"
                     /translation="MVIGMTIMVFFNFFNVVCRYLLPQTPFSYTEELVILVFIWVSMF
                     GISYGYRKGSHTILNVFTDRLPFRFQPFVIFFAAASSAVLMLLFAYTGYRMVMN"
BASE COUNT          296 a          377 c          326 g          290 t
ORIGIN      
        1 aagggcaagt tcgccatcac catctatccc agggacgaac tgtccggggg caaccaggcg
       61 gccggaatcg agctgcttca gataggggcc acggatatcc accttcagga cgcccttgtc
      121 tggtcctcca ttgccaaaaa gagcattatc ccctgcttcc cgtggcttct gcccaccaat
      181 gccgaggtgg acaagtacgt catgaacggg gaagggggca aggcactcag gcagatcatt
      241 tccgacgccg ggtgcgtctg cctggccgta ggtgagaacg ggtaccgcca ggtagccaac
      301 aaccggaatc ccattaagat gcccgaagac atgaagggtc tcaaaatacg ggttcccgga
      361 agcaacgtcc acgtgaactt gctgaagtac atcggcgccg accccctgac catgaaccag
      421 agcgaggtgt acacctcgct ccagcagggt gccatcgacg cgtgtgaaaa cacccttgac
      481 cttctcttta cccagaacac cctggaagtg gtatcccacc tgacattgtg gaattactcc
      541 tacgatcccc ttttcctctc ggtcagcaat aagctctgga attcactgag cgacgaagaa
      601 aaggtcattt tccaggagtg cgccgaagag gcaatgaagc accaggtgaa ggccgcccgg
      661 gagaccgtgg aaagcctcat gaagaatttc ggcaaatacc ccaaggtcca ggtcgtgacg
      721 agcctcaccg aagaccaggt tgcggctttc aaaaaggccg tggcccccat ttacgaggat
      781 taccgcaaga ccttcggcga ggaccttttc gcgaagttcg gatacacatt ctagaagcct
      841 atgcaggaag gctgcaggga gagtatggag acatgctctc cctctttttt tcggttcacc
      901 atgccccgtc cctgggatat ccttcccccg gacacgccac ggaggtaaag atgaaaagac
      961 tatggtcttt ccttaacata ttcgaagaat ccatcatggt tatcggaatg acgatcatgg
     1021 tgtttttcaa tttcttcaac gtcgtgtgcc gctaccttct gccccagact cctttttcct
     1081 ataccgagga gctggttatc ctcgtgttca tctgggtcag catgttcggc atttcctacg
     1141 gctaccgcaa ggggtcccac acgatcctca atgttttcac cgacaggctt cctttccgct
     1201 ttcagccctt tgtcatcttt tttgccgcag ccagctcggc cgttctcatg ctgctgttcg
     1261 cctatacggg ataccgcatg gtcatgaac
//
LOCUS       DAOTHD010000769         1286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_126030_length_1286_cov_3.413485, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000769 DAOTHD010000000
VERSION     DAOTHD010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126030_length_1286_cov_3.413485"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="P5219_11225"
     CDS             complement(<1..806)
                     /locus_tag="P5219_11225"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HRX27380.1"
                     /translation="MAKQEHREDISLAGALTALGGILAILAGGTLTFGIHIAVLLALC
                     GVFTTGLFRLKYGFGWRLLFIDGVLPMVSRASGALGILLSVGPLIAAWTLSGTIPYLV
                     FTGLDILTPRIFLPAAFLLCSFASLLTGTSWGTAATFGVALTAIAGGLGIPAAAAAGA
                     IVGGAYFGDKLSPVSDTTVLAAAVAEVDIMDHIRSTLWTTLPGAALSVAVYAAAGSGP
                     GGAMDNAGIGAMTSALSGRFFLGPVLLLPPLVLLVMAWKRFPTLAALWTSV"
     gene            923..>1286
                     /locus_tag="P5219_11230"
     CDS             923..>1286
                     /locus_tag="P5219_11230"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HRX27381.1"
                     /translation="MADFHHSLPPYGVEFGRRKTLQISVHPDGRVTVKAPLFLDRAAV
                     DRAVEEKREWIGKKLDHFRSLPAPAYRPPLGQGSPVPFLGQLYPLHISEGKRFDALIE
                     GGALRITLRKGTEPEASLP"
BASE COUNT          268 a          393 c          445 g          180 t
ORIGIN      
        1 acggaggtcc acagggccgc cagggtggga aaccgtttcc atgccatgac caggaggacc
       61 agggggggaa ggagcaggac ggggccgagg aagaaccggc ccgacagggc cgacgtcatg
      121 gctcctatgc ccgcgttgtc catggcccct cccgggccgg atccggcggc ggcgtagacg
      181 gccacgctca gggccgcgcc gggaagggtg gtccagaggg tggagcggat atggtccatg
      241 atgtccactt cggccacggc ggcggcgagg accgtggtgt ccgaaacggg gctcagcttg
      301 tcgccgaaat aggcgccccc gacgatagcc cccgccgccg ccgcggcagg aatgccgaga
      361 ccgcccgcga tggcggtgag ggccacaccg aaggtggccg ccgttcccca ggacgttccc
      421 gtgagcaggg aggcgaagga gcagagcaga aaggccgcgg gcaggaagat ccggggcgtg
      481 agaatgtcca gccccgtgaa aaccaggtag ggaatggtgc cggagagggt ccatgcggca
      541 atgaggggcc ccacgctgag gagaattccc agggctccgg aggcccgtga aaccatggga
      601 agcacgccgt cgatgaagag gagcctccac ccaaaaccgt atttcagcct gaaaaggccc
      661 gttgtgaaga ccccgcaaag ggccagcagc acggcgatat ggattccgaa cgtcagggtt
      721 cccccggcga gaatggccag gatgccgccg agggcggtga gcgccccggc aagagaaata
      781 tcctcccggt gttcctgttt ggccatggcc gttcctcctc tcccgctttt tcttccggaa
      841 gaacactata ccagagaaag gcgtgccggt aaggccgaaa aaatggtagg atgtcatgaa
      901 gaacagcgaa ggagggtccc ccgtggcaga ttttcaccac agtcttcccc cctatggggt
      961 ggaattcggc aggcgaaaga ccctgcagat ttcggtccac cctgacgggc gggtaacggt
     1021 gaaggctccc ctgttcctcg acagggccgc cgtggacagg gcagtcgagg aaaagcggga
     1081 gtggatagga aaaaaactgg accatttccg ctccctcccc gcaccggcgt accggcctcc
     1141 cctggggcag ggaagcccgg tccccttcct tgggcagctc tatcccctcc atatcagtga
     1201 aggaaagcgg ttcgacgccc tgatcgaggg cggcgccctg cggatcaccc tgcggaaggg
     1261 cacggaaccg gaagcgtccc tcccgg
//
LOCUS       DAOTHD010000770         1284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_126346_length_1284_cov_3.342555, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000770 DAOTHD010000000
VERSION     DAOTHD010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126346_length_1284_cov_3.342555"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="P5219_11235"
     CDS             complement(<1..250)
                     /locus_tag="P5219_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRX27382.1"
                     /translation="MEFQFSLAQLTVLNTSPAEIASMAADCGYDFVSMRQIYMGLPGE
                     PDYDLAKNRALMKETKKVFSDTGIKLLDIELARVFDGMD"
     gene            complement(507..>1284)
                     /locus_tag="P5219_11240"
     CDS             complement(507..>1284)
                     /locus_tag="P5219_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRX27383.1"
                     /translation="FVANQPADGYSILFGAENPNLYKVTGLSQIDYDQFEPVFLMMAN
                     VGVVIVSKDSPYTSYKELIEAAASGKTVTMGSTGPGGLPYVATTMIEKIHGVKFNKMQ
                     FDGEGPCITALMGGHIDAVAVGLLAAANFIHSGNVKGLAMISSERIDSVASVPAVTEV
                     YEEYRPYLPWGAFFGAFVRKETPAEALDVLRSAFTKAFQDPRFDEFAKTMGGVKLGIT
                     GDEAKKYIRQNQSVAAWLLYDAGGAKFSPEEFGIERPQGK"
BASE COUNT          275 a          371 c          375 g          263 t
ORIGIN      
        1 cgtccatgcc gtcgaacacc ctggcaagtt caatatccag gagcttaatg cccgtatcgg
       61 aaaaaacctt cttcgtctct ttcatcagag cccggttctt cgccaggtcg taatccggtt
      121 ctccgggaag ccccatgtaa atctgcctca tgctcacgaa atcgtacccg cagtccgccg
      181 ccatggacgc tatctccgcc ggagacgtat tcagaacggt gagctgcgca agggaaaact
      241 gaaactccat gaacatcata ccccttcctc gtaagtggtg tccgggcact gaacgccctt
      301 tccgggagcg aaagaccgct ccgcccaaaa ttccgcttaa tggaaatgaa atccgcctct
      361 aggaaacatc tccacaataa atactgggat agaatttgtc aatatggatg ccggaaaaag
      421 ggtacttttt tgggggaaat gggaacagaa aaaagcccgc cccgggaagg gacgggccgg
      481 aacaccattc acacggaaaa gcgtctctac ttgccctggg gcctttcgat gccgaactcc
      541 tccggggaga acttcgcccc gccggcgtcg tacaggagcc atgctgccac ggactggttc
      601 tgcctgatgt atttcttcgc ttcgtcgccg gttatgccga gcttgacgcc gcccatggtc
      661 ttcgcgaact cgtcgaacct tggatcctgg aacgccttgg tgaaggcgga ccggagaacg
      721 tcaagagcct cggcgggtgt ctccttcctc acgaaggcgc cgaagaaggc gccccagggg
      781 aggtagggcc tgtactcctc gtagacttcc gtgaccgccg gcaccgaggc cacgctgtct
      841 atcctctcgg aggagatcat ggcaaggcct ttcacattgc ctgaatgaat gaagttcgct
      901 gcggcaagaa ggccgacagc caccgcatcg atatggccgc ccatgagggc ggtgatgcag
      961 ggaccttcac cgtcgaactg catcttgttg aatttcacgc cgtggatctt ctcgatcatg
     1021 gtggtggcca cgtagggcag gcctccgggg ccggtggagc ccatggtgac ggtcttgccg
     1081 gaagcggcgg cctcgatgag ctccttgtag gacgtgtagg gggaatcctt ggagacgatc
     1141 accacgccca cgttggccat catgaggaag accggctcga actggtcgta gtcgatctgg
     1201 gaaaggccgg tgaccttgta gaggttcggg ttctccgcgc cgaagagaat gctgtagccg
     1261 tcggcgggct ggtttgccac gaag
//
LOCUS       DAOTHD010000771         1283 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_126502_length_1283_cov_2.978013, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000771 DAOTHD010000000
VERSION     DAOTHD010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126502_length_1283_cov_2.978013"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="P5219_11245"
     CDS             <1..727
                     /locus_tag="P5219_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase"
                     /protein_id="HRX27384.1"
                     /translation="ALSDPVFVTAYDIPPEEHVEVTGIFARVVDSGISKTVNLPSGAT
                     VDEVKQIYRRCYAMGAKGITIYRDGSRSFQPIETAKKPEEQVPHTSRVKERPGLVVFG
                     KTIKEQTPWGSIYVTLNFDGTEPFEVFATIGKSGSELKAMTEALSRAISIGLRSGGKL
                     EDFIATLKGLSGKEYWLFEFDDKHVARSIPDAIAVLLEKLIGKDGDVHGAEGGVACPE
                     CGAPMEMISGCEYCFSCGYSPCK"
     gene            complement(780..980)
                     /locus_tag="P5219_11250"
     CDS             complement(780..980)
                     /locus_tag="P5219_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27385.1"
                     /translation="MKITREEYRVLFPQGREVQAPQQVRDERKISGRREEERAEENLQ
                     ARETRNRELADLGLGTKLNLEG"
     gene            complement(1021..>1283)
                     /locus_tag="P5219_11255"
     CDS             complement(1021..>1283)
                     /locus_tag="P5219_11255"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HRX27386.1"
                     /translation="SDGRVRLSALRGDRTLERVLDNFQVKAAMHSRYLQFTVQEDADI
                     VQVIVRESNDEEKIIRKIPSDEIVRLIARRNEILGFLFDMVV"
BASE COUNT          240 a          394 c          379 g          270 t
ORIGIN      
        1 cgccctttcc gacccggtct ttgtgacggc ctacgacatt cctccggagg agcacgtgga
       61 ggtgacgggc attttcgccc gggtggtgga cagcggcatc agcaagacgg tgaaccttcc
      121 ctccggagcc actgtggacg aagtgaagca gatctaccgc aggtgctacg ccatgggagc
      181 caagggcatc accatctacc gggacggttc ccggtccttc cagcccatcg agacggcgaa
      241 gaagcccgag gagcaggtgc cccacacaag ccgggtgaag gagcgcccgg gactcgtggt
      301 gttcggaaag accatcaagg agcagacgcc ctggggcagc atctacgtga ccctgaattt
      361 cgacggtacc gagcctttcg aagtcttcgc caccatcgga aagagcggtt ccgagctgaa
      421 ggccatgacg gaggcccttt caagggccat ctccataggc ctccggagcg gaggaaagct
      481 cgaggatttc attgctaccc tgaaagggct ctcaggcaag gagtactggc tgttcgaatt
      541 cgacgacaag cacgtggccc gctcaattcc ggacgccatc gccgtcctgc ttgaaaaact
      601 gatagggaag gacggcgacg ttcacggagc cgaaggcggc gttgcttgcc cggaatgcgg
      661 ggctcccatg gaaatgatct caggctgcga atactgcttc agctgcggct attccccctg
      721 caagtagggt gcaaaaaagg ggcggggatt gttctccccg cccctgcttt ctctctgctt
      781 cagccttcca ggttgagctt cgttcccagc cccagatctg caagctccct gtttcttgtc
      841 tcccgggcct gaaggttttc ttcagcccgt tcctcttcgc gcctgccgga gatcttccgt
      901 tcgtcccgca cctgctgggg tgcctggacc tccctgccct gcggaaagag gacccggtac
      961 tcctccctgg ttatcttcat cggacgctca tcgctccttt ctcccgtggg atcatcccga
     1021 ctaaacgacc atatcgaaga ggaagccgag aatctcgttt cttctggcta tgagccgcac
     1081 tatttcatcg gaaggtattt tgcggatgat cttttcctcg tcgttgctct ccctgacgat
     1141 gacctgcacg atgtcggcgt cttcctggac ggtgaactgg aggtaccggc tgtgcatggc
     1201 ggccttgacc tggaagttgt cgagaacgcg ctccagagtc ctgtcgcccc ggagggcgct
     1261 caggcggacc cggccgtcgg aga
//
LOCUS       DAOTHD010000772         1283 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_126503_length_1283_cov_2.960098, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000772 DAOTHD010000000
VERSION     DAOTHD010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126503_length_1283_cov_2.960098"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="P5219_11260"
     CDS             complement(<1..669)
                     /locus_tag="P5219_11260"
                     /inference="COORDINATES: protein motif:HMM:NF024606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB N-terminal domain-containing protein"
                     /protein_id="HRX27387.1"
                     /translation="MELFLVALAMTVLYRIARALFRLAAGRRERESREKLLKDAETGR
                     PGTAEPARWFGSMETPAVNGYSVPGGLVYVGEKLLDYSGFNDPCLLNPSLRAVSGFSP
                     SVPLPEQRLHYGKMTPEQRGAYLSWLAGGRNDPDAALPYVFLFFYGLERRLMVDAQRG
                     AVSPEERADIVLEILRLLQIYGEHRAFRGHAANLLAMEWVLWGIPDDRPGYIDFTDRY
                     CAEPF"
     gene            complement(704..>1283)
                     /locus_tag="P5219_11265"
     CDS             complement(704..>1283)
                     /locus_tag="P5219_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27388.1"
                     /translation="AGAAALKRYKIPFLVFPVAATLWYMSMDLAPFLFRDADLTWRLR
                     RIVSLWFGLAMILFALRVDFRTRSKGDFAFWLYLFGLAAFWGGLSLLDSGSELGRFFY
                     LCVNLLLIAAGAVLSRRVFVVFGGLGTSGYIGHLAWRVFENSALFPFVLSFVGFAVIW
                     LGILWQRREKQVTAALRSFLPVRVREFLENGR"
BASE COUNT          302 a          396 c          379 g          206 t
ORIGIN      
        1 aaaaggttcc gcgcagtacc tgtcggtgaa gtcgatgtac cccggccggt cgtcgggaat
       61 cccccagagg acccactcca tggcgagaag gttggcggcg tgcccccgga acgcccggtg
      121 ctctccgtaa atctgaagca gccggaggat ttccagcacg atgtccgcac gctcttccgg
      181 ggatactgct ccccgctggg cgtccaccat gaggcgccgc tcgaggccgt aaaagaacag
      241 gaaaacatag ggaagggcag cgtcaggatc attccttccc cctgcgagcc atgacaggta
      301 ggccccccgc tgctccggcg tcatttttcc gtagtggagc ctctgctccg gaagcggcac
      361 cgacggtgag aatccggaaa ccgccctgag cgaggggttg agaaggcagg gatcgttgaa
      421 tcccgaatag tccaggagct tttccccaac gtagaccagc cctccgggaa cggaatatcc
      481 gttcaccgcc ggggtttcca tggagccgaa ccaccgggcg ggttccgctg taccgggcct
      541 gcccgtttcg gcatccttca ggagcttttc gcgggattcc cgctccctcc tgcctgcggc
      601 gaggcgaaac aacgccctgg cgatcctata cagtacggtc atggcgagag cgacaagaaa
      661 aagctccaat agagcaacct cttttcatcg aaatttccac tccctatcgt ccgttttcga
      721 gaaattccct cacccggacg ggcaggaaag acctgagcgc cgccgtcacc tgtttctccc
      781 tgcgctgcca gagtatgccg agccagatca cggcaaaacc gacaaaagaa agaacgaagg
      841 ggaaaagggc gctgttctcg aacaccctcc aggcaaggtg tccgatgtat cccgaggtcc
      901 cgaggcctcc gaagacgacg aaaacccgcc gggaaagcac cgccccggcg gcgatgagga
      961 gcaggttgac gcagagatag aaaaaccgcc cgagttcgct tccggaatcc aggagggaca
     1021 gccctcccca gaaagcggcc agtccgaaaa gatacagcca gaaggcgaaa tctcccttcg
     1081 aacgggtcct gaaatccacc cggagggcga agaggatcat ggcgagaccg aaccagaggg
     1141 acacgatcct acggagcctc catgtgagat ctgcatcccg gaacaggaag ggagcgagat
     1201 ccatgctcat gtaccagaga gtggccgcga cgggaaaaac gaggaagggt attttgtacc
     1261 gcttcaaggc cgcagctcct gcg
//
LOCUS       DAOTHD010000773         1282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_126705_length_1282_cov_2.030155, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000773 DAOTHD010000000
VERSION     DAOTHD010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126705_length_1282_cov_2.030155"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(250..1236)
                     /locus_tag="P5219_11270"
     CDS             complement(250..1236)
                     /locus_tag="P5219_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRX27389.1"
                     /translation="MQTLFRGKHFITLQEWTKEEIDTLLDVSCDLKMQFALDRPTDYL
                     PNKTVFLMFFEQSTRTRNSMEAGITQLGGHAHFLDTSNMQIAHGEVPKDTAVILSRMG
                     HAIACRNCFWKEGNAYLREMAKWSPVPIISMQDDLYHPLQGIADLMTIQEKRGKNTRG
                     LKVSVIWAYATTHKKPISVPLSQALLFPRYGMEVTLAYPKGYEMPDWVIEQAKKNALD
                     NGGTFRITNDQEEAYRDADVVIPKNWGNWVTNESKAVIDSTLEANKHWKCTEQRMALT
                     DKHSLYMHALPADRVNEVEDSVIDGPHSAIYDEAENRLHTAKAVMALTMGGR"
BASE COUNT          214 a          356 c          397 g          315 t
ORIGIN      
        1 cgtggcgttc cagctctggt cgttgatggg gcccgggagg atcagcacca ttttatgatc
       61 cccgggggcc ttcgcgaaag ccggcacccc gtggaaaatc cccgccgaca ccatagccgc
      121 aagcactgca agcagaacga accgcttcat ccttctccct ccttgacgat aatgatgatt
      181 tcagcaggag tatagcatga agaaaaaagg aggatgcccc tgttgtccgg catcctccca
      241 aaaatttttt taccttccgc ccatggtgag ggccatgacg gcttttgccg tatggagcct
      301 gttttccgcc tcatcgtaga tggcggagtg aggcccgtcg atgaccgagt cttccacttc
      361 gttcaccctg tcggcgggga gggcgtgcat gtacagggaa tgcttgtcgg tgagggccat
      421 gcgctgctcc gtgcatttcc agtgcttgtt tgcctcaagg gtgctgtcga tgacggcctt
      481 gctctcgttc gtcacccagt tgccccagtt cttgggaatg accacgtcgg catcccggta
      541 ggcctcttcc tggtcgttgg tgatccggaa ggttccgccg ttgtcaaggg cgttcttctt
      601 tgcctgctcg atgacccagt cgggcatctc gtagcccttc gggtaggcaa gggtgacttc
      661 catgccgtac ctggggaaca ggagggcctg gctgaggggt acggagatgg gcttcttgtg
      721 ggttgtggcg taggcccaga tgacggagac cttgagtccc cgggtattct ttcccctttt
      781 ctcctggatg gtcatgaggt cggcgatgcc ctgcaggggg tggtacaggt cgtcctgcat
      841 gctgatgatg ggcacgggag accacttggc catctcccgg agataggcgt tcccttcctt
      901 ccagaagcag ttcctgcacg caatggcgtg acccatgcgg gacaggatga cggccgtgtc
      961 cttgggcacc tcgccgtggg cgatctgcat gttgctggtg tccaggaagt gggcgtggcc
     1021 gccgagctgg gttattcctg cctccatgga gttcctggtc cgggtggact gctcgaagaa
     1081 catgaggaac acggtcttgt tgggcagata gtccgtcggc ctgtcgaggg cgaactgcat
     1141 cttcaggtcg caggaaacat cgagcagggt gtctatctct tctttggtcc actcctggag
     1201 ggtaatgaag tgtttccccc ggaaaagcgt ctgcatgatt ctctctcctc cttgaatgcc
     1261 cgctctagcg ggcgtgtttt tc
//
LOCUS       DAOTHD010000774         1280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_126907_length_1280_cov_4.217959, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000774 DAOTHD010000000
VERSION     DAOTHD010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126907_length_1280_cov_4.217959"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="P5219_11275"
     CDS             <1..809
                     /locus_tag="P5219_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRX27390.1"
                     /translation="LGALSAFNACQPTYREVDTDGEGMVIADLEKALAEEKRAKLIYV
                     IPDFQNPSGKTWSLERRKAFMETVSQHDILVVEDNPYGELRFEGTTPPSLKSMDTKGQ
                     VVVLGTFSKIFCPGLRVAWVCGEPSFLESFCNLKQAADLHTSTLSQYEIDAFLSEYDI
                     EEHVRKLVSVYRERRDIMIRTMDEAFPAEVTFTRPEGGLFTWAEMPEKISGRDLFMTA
                     LEKKVAFVPGGAFYPNGGHENTMRLNYSNMPPEKIVEGIRRLGEALREAL"
     gene            complement(905..>1280)
                     /locus_tag="P5219_11280"
     CDS             complement(905..>1280)
                     /locus_tag="P5219_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HRX27391.1"
                     /translation="KSILVSISKAVNSSIQLRSKKELIEHFIGTVNASTNVDEDWRKF
                     VREQKETDLNVIIGEEKLKEEETRKFLNNSFRDGTLKTTGTDIDKILPPASRFGGGTR
                     AAKKQSVIARLMQFFEKYLGLV"
BASE COUNT          250 a          407 c          374 g          249 t
ORIGIN      
        1 acctcggagc cctgtcggcc ttcaacgcct gccagcccac ctaccgggaa gtggacaccg
       61 acggcgaggg catggtgatc gccgacctgg agaaggccct ggcggaagag aagcgggcca
      121 agctcattta cgtgatcccc gatttccaga acccctccgg aaagacatgg tctctcgaac
      181 ggcggaaggc cttcatggag accgtttcac agcatgacat cctcgtggtg gaggacaacc
      241 cctacggcga actccggttc gagggaacca cgcccccatc cctgaaatcc atggacacga
      301 agggacaggt ggtggtcctg ggcaccttct ccaagatctt ctgccccggc ctccgggtgg
      361 cctgggtctg cggcgagccg tccttcctcg agagcttctg caacctgaag caggcggcgg
      421 acctccacac ctccaccctc agccagtacg agatcgacgc ctttctgtcg gagtacgaca
      481 tcgaggagca tgtccggaag ctggtctcgg tctaccggga gcggcgggac atcatgatcc
      541 gcaccatgga cgaagccttc cccgcagaag tgacgttcac caggcccgag ggcggcctct
      601 tcacctgggc cgagatgccc gaaaagatca gcggaaggga tctcttcatg acggccctgg
      661 agaagaaagt tgccttcgtc cccggcggag ccttctaccc caacggcggc cacgagaaca
      721 ccatgcgact caactactcc aacatgcccc ccgagaagat cgtggagggc atccggcgcc
      781 tcggggaagc cctacgggaa gccctgtaaa ctgtaaaccc gaaatacaca gagacagcag
      841 gaacccggcc gcggtcaggc atttatgcct gccgaggccg ggttcttttc tttcagcggc
      901 ggctctagac cagccccagg tacttctcga aaaactgcat gagccgggcg atgacgcttt
      961 gctttttggc cgcccgtgtc ccgccgccga agcgcgaagc cgggggcagg atcttgtcga
     1021 tgtccgtgcc ggtggttttc agcgttccgt cccggaagga attgttcagg aatttccgtg
     1081 tctcttcctc cttcagcttt tcctcgccga tgatcacgtt gaggtcggtc tccttctgtt
     1141 cccggacgaa cttccgccag tcctcatcca cattggtgga ggcgttgacg gtcccgatga
     1201 agtgctcgat cagctccttc ttgctgcgga gctggatgct tgagttgacc gccttggaga
     1261 tggagaccag tatgctcttg
//
LOCUS       DAOTHD010000775         1279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_127080_length_1279_cov_3.086601, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000775 DAOTHD010000000
VERSION     DAOTHD010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127080_length_1279_cov_3.086601"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="P5219_11285"
     CDS             <1..394
                     /locus_tag="P5219_11285"
                     /inference="COORDINATES: protein motif:HMM:NF014822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HRX27392.1"
                     /translation="GISLLVAELNRVYRQETALHERDFTPDGFEWCDFSDWEKSVVSF
                     VRKSVSGDMVLAVFNFTPVPRLHYRIPVPCGGFWREILNSDAKEYGGSGFGNYGGLHS
                     EELRWKESGHSLCLTVPPLGMLLLKLER"
     gene            complement(445..>1279)
                     /locus_tag="P5219_11290"
     CDS             complement(445..>1279)
                     /locus_tag="P5219_11290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HRX27393.1"
                     /translation="SRAMTGKVTREALDTFKDRDGVSAAEAMKAFGFDPDRIGEAIRT
                     EGSLKAFIEMHIEQGPILESEGTDLGLVKTIVGISQREVEILGRPDHAGTTPMDMRCN
                     ALLAASEAVLFLDRAAKEAAGGTVGTVGRMEVFPGGANIVPGRVFFTIDVRSADMSKL
                     EQVTGSFAAFLKDLEKKHGVTISLTDKLTVRPVEMSAEILGLFGAEAAKRGYSSKVMQ
                     SGAGHDAMIMASAADVGLVFVPSKGGRSHCPEEWTDYGQIKKGVDVVLGAVLSLAKAA
                     V"
BASE COUNT          199 a          431 c          391 g          258 t
ORIGIN      
        1 ggggatttcc cttctcgtgg cggagctgaa ccgggtgtac cggcaggaaa ccgccctcca
       61 cgaacgggat ttcactcccg atggcttcga gtggtgcgat ttctccgact gggaaaagag
      121 cgtggtttcc ttcgtccgga agagcgtttc cggcgacatg gtcctggccg tgttcaactt
      181 tacccctgtg cccaggctcc attaccgcat tcccgttccc tgcggaggct tctggcggga
      241 aatcctcaac agcgacgcaa aggaatacgg cggatcgggg ttcggcaact acgggggcct
      301 tcactcggag gagcttcggt ggaaagagag cggacactcc ctgtgcctca ccgttcctcc
      361 cctggggatg ctgctgctga agctggaacg gtaaagaacg gggcggaccc ggacgggtcc
      421 gccccttgtc aattgcggtg tttttcagac cgcggccttc gccagggaga gcaccgcgcc
      481 gaggaccacg tccacgccct tcttgatctg gccgtagtcg gtccactcct ccgggcagtg
      541 gctccgccct cccttgctgg ggacgaagac caggcccacg tccgcggccg atgccatgat
      601 catggcgtcg tgccctgctc cgctctgcat gaccttcgac gagtagcccc gcttcgccgc
      661 ctctgccccg aaaaggccga gaatctccgc cgacatctcc acgggccgga cggtgagctt
      721 gtcggtgagg gatatggtga cgccgtgctt tttctccagg tccttcagga aggcggcgaa
      781 ggacccggtc acctgttcga gcttcgacat gtccgccgac cggacgtcga tggtgaagaa
      841 caccctcccc ggaacgatgt tcgccccgcc ggggaagact tccatcctcc ccacggtccc
      901 caccgttccc ccggcggcct ccttcgccgc ccggtcgagg aagagtaccg cctcggaggc
      961 cgcgaggagg gcgttgcacc gcatgtccat gggagtggtc ccggcatggt cgggtctgcc
     1021 gagaatctcc acttcccgct ggctgatgcc cacgattgtc ttgaccaggc cgaggtccgt
     1081 tccctcgctc tcgaggatgg ggccctgctc gatgtgcatt tcgatgaagg ccttcagact
     1141 gccctcggtc cggatcgcct ctccgattct gtcgggatcg aagccgaagg ctttcattgc
     1201 ttccgcggcg gacaccccgt cccggtcctt gaaggtatcc agggcctccc tggtgacctt
     1261 gcccgtcatg gccctgctc
//
LOCUS       DAOTHD010000776         1277 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_127414_length_1277_cov_2.865794, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000776 DAOTHD010000000
VERSION     DAOTHD010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127414_length_1277_cov_2.865794"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..>1277)
                     /locus_tag="P5219_11295"
     CDS             complement(87..>1277)
                     /locus_tag="P5219_11295"
                     /inference="COORDINATES: protein motif:HMM:NF026952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB C-terminal
                     domain-containing protein"
                     /protein_id="HRX27394.1"
                     /translation="ALAAGCADDLDGYSRFLARGGSPESPEAAALLPAELLTGAPAGE
                     AVRERLFRLCGGNPSMTDTGKILAVLGQTPPPKMNRKRAATLADTLGSFNFGMAPDPH
                     IHGFPPSHDGRIALFSPRAVPSGREEFDALTALIRLGSLVAQASGEASEGERRVVEEM
                     VSSSDALSPEEKRSLFALALWAFSVPQGAAGLRGILEPLPEETKRSLGRILGTIAAAD
                     GRVDPGEVRAVGKLYGYLGLDGGQAARDIHAAVSGTASSARSEEKEGFSLDMDLIRLR
                     QEETRHVNGVLREIFSSAEENALPAQEAPERDDILAGLDDPHRKLLSDLLKRETWRRE
                     EYESLCATLSLPPDGALEILNEWAFSSAGAPLAEDGDPVFIDLSLAEEVLSHGQQKDE
                     NPAP"
BASE COUNT          222 a          405 c          430 g          220 t
ORIGIN      
        1 tggtcaaatt gcattcctcc cggacggcat gccgccccgt gcggggggtc acccccgcac
       61 ggagggactg gagaatggcg tcccgctcac ggggccggat tttcgtcttt ttgctgtcca
      121 tgggaaagaa cctcctctgc cagggatagg tcgataaaga cggggtcccc gtcctcggcg
      181 aggggcgccc ccgccgagga aaaggcccat tcgttcagta tttccagtgc cccgtcgggg
      241 gggagggaca gggtggcgca gagagattcg tactcctccc tccgccaggt ttcccgcttc
      301 agaaggtcgg agagcagctt ccggtgcggg tcatcaaggc cggcgagaat atcgtccctc
      361 tccggcgcct cctgtgcggg gagggcattt tcttccgctg aagagaatat ctcccggaga
      421 acgccgttca catgccgggt ttcctcctgc cggaggcgga tgagatccat gtccagggaa
      481 aatccttctt tttcctcact tcgggcggac gaagccgtgc cggatacggc ggcgtggatg
      541 tcacgggcgg cctggccgcc gtcaagaccg aggtatccgt agagcttccc tacggcgcgg
      601 acctccccgg ggtccaccct gccgtctgcg gctgcaatgg tgccgagaat acggccaagg
      661 gaacgtttcg tctcctccgg aaggggttcc aggatccccc tgagacccgc cgcgccctgg
      721 ggcacggaaa aggcccagag cgcaagggcg aagagggacc gcttctcctc cggagaaagg
      781 gcatcactgg aagagaccat ttcctccact accctgcgct ccccttccga cgcctccccc
      841 gacgcctgtg ccaccagact tcccaggcgg atcagcgcag tcagggcatc gaattcctcc
      901 cggccggagg ggaccgcccg gggagaaaag agagcgatcc ggccgtcatg ggacggggga
      961 aagccgtgaa tatggggatc aggggccatg ccgaagttga agcttcccag ggtgtcggcg
     1021 agtgtcgccg cccgtttgcg gttcattttg ggagggggag tctgcccaag gacagcgagg
     1081 atcttccccg tgtcagtcat ggaagggttt cccccacaga gccggaagag cctttcccgg
     1141 accgcctccc cggccggggc gccggtgagc agctccgccg gaagaagggc ggcggcctcg
     1201 ggagattcgg ggcttcctcc acgggcaagg aatctgctgt agccgtcaag gtcgtcggcg
     1261 cacccggcgg cgagggc
//
LOCUS       DAOTHD010000777         1274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_127872_length_1274_cov_3.118950, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000777 DAOTHD010000000
VERSION     DAOTHD010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127872_length_1274_cov_3.118950"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="P5219_11300"
     CDS             complement(<1..920)
                     /locus_tag="P5219_11300"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRX27395.1"
                     /translation="MPVPRTIPTTTSVSDPMMDKKKGERPLDGYRVLEMGQLLAGPFA
                     ASVLGYFGAEVIKIERPDGGDDTRAYPPFQKGESAYFMSLNRGKRSVTLNLKHPRGKE
                     ILKDLAKKSDILIENFKPGTMDKLGVGYEDLRKVNPRLIYAASSGFGQTGPYSSRPAY
                     DLIIQGMGGMMSVTGPDEHTPTKVGSSVADIFAGLFTVIGVLSAVNARNRTGRGQLVD
                     VAMLDCMVAVLENAVSRYLVSGEIPRPIGNRHPSISPFTTLATSDGAMNIAVGNDEIW
                     KRMCAVLGMEEYVNDPRFVDNPARVENFHAL"
     gene            complement(957..>1274)
                     /locus_tag="P5219_11305"
     CDS             complement(957..>1274)
                     /locus_tag="P5219_11305"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRX27396.1"
                     /translation="CVVSTWTLCSIPDLDRALAEIRRVLKPDGQLLFLEHGRAPDAAV
                     ARWQDRLAPVFRGLAGCNPNRQIDASIEAAGFRFTDIERSYFDGPRFISWHFVGKATP
                     DRR"
BASE COUNT          227 a          373 c          411 g          263 t
ORIGIN      
        1 agggcgtgaa aattctccac tctggcgggg ttgtcgacaa agcggggatc gttcacgtac
       61 tcttccatgc cgagcacggc gcacattctc ttccagattt cgtcgttccc cacggcgatg
      121 ttcattgcgc cgtcggacgt cgcgagggtg gtgaaagggg atatggaggg atgacggttg
      181 ccgatggggc gcgggatctc gccgctgacc aggtacctgg agacggcgtt ttcgaggacg
      241 gccaccatgc agtcgagcat ggccacatcg acgagctgcc ccctgccggt ccggttccgg
      301 gcgttcacgg cggaaaggac gccgatgacg gtgaacagac cggcgaaaat atccgccacg
      361 gagctgccga ctttcgtggg ggtgtgctcg tccggtccgg tgacgctcat catgccgccc
      421 atgccctgga tgatgaggtc ataggcgggg cgggagctgt agggtcccgt ctggccgaag
      481 cccgagcttg cggcgtagat gagccgggga ttgaccttcc tgagatcttc gtatccgacg
      541 ccgagtttgt ccatggttcc cggcttgaag ttttctatga ggatgtcgct ttttttcgca
      601 aggtccttca ggatctcctt gcccctgggg tgtttcaggt tcagcgtaac gcttctcttg
      661 ccccggttga ggctcatgaa ataggcgctc tcgcccttct ggaagggagg ataggcacgg
      721 gtatcgtctc ctccgtcggg acgttcgatc ttgatgacct cggcaccaaa atagccaagt
      781 acgctggccg caaaagggcc ggccagcaac tggcccattt cgagaacgcg gtagccgtcg
      841 aggggcctct cgcccttctt cttgtccatc attggatcgc tcactgaggt cgtagtgggg
      901 atggtccgtg gaaccggcat tctgccagtt ccgatgctgc ctgcaaaacc gccaggctag
      961 cgcctgtccg gcgtggcctt gccgacgaag tgccaggaga tgaatcgtgg cccgtcaaaa
     1021 tagctgcgtt cgatgtccgt aaagcgaaat ccggcagctt caatcgaggc gtcgatttgc
     1081 cggttcgggt tgcagccggc cagtccgcga aataccggcg cgagccggtc ctgccagcgt
     1141 gcaacggcgg catcgggagc ccggccatgc tcgaggaaca gcagctgccc gtccggcttc
     1201 agcacacgcc ggatctcggc cagggcacgg tccagatcgg ggatcgagca aagcgtccag
     1261 gtactcacta caca
//
LOCUS       DAOTHD010000778         1274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_127874_length_1274_cov_3.116489, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000778 DAOTHD010000000
VERSION     DAOTHD010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127874_length_1274_cov_3.116489"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="P5219_11310"
     CDS             complement(<1..938)
                     /locus_tag="P5219_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX27397.1"
                     /translation="MDSTAPDRTGNETIMVVDDTPTNLTILVELLGRRGYRVAAFPRG
                     GMALKAAALEAPDLVLLDIMMPDMDGFEICRRLREDERLREVPVLFISALDDPAGKVR
                     AFAAGGVDYVTKPFQEEELAARVRTHLTLVRMRKELERHNHYLEDLVREKVREISESQ
                     LATILALSKLAESRDDATGKHIERTRTYCRLIALTLREDPRFRETVTDEFVENIFHAA
                     PLHDIGKVGIPDRILLKPGKLTPEEYEIMKTHAALGAGTLERVLEEYPGNALVKMGIT
                     LTRYHHEKWDGTGYPEGLAGENIPLGGRIMALADVY"
     gene            complement(931..>1274)
                     /locus_tag="P5219_11315"
     CDS             complement(931..>1274)
                     /locus_tag="P5219_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27398.1"
                     /translation="PVLTDHDREGTPGRDSLVSKEIGAVPRELPGEKISAESTPVPGG
                     DFPSVLPEGIRSGMVRALERGDILRLRELAAEAAASDETAGKRLRDLVAAFDYEQLAR
                     ILGMEEDAFHG"
BASE COUNT          203 a          416 c          374 g          281 t
ORIGIN      
        1 tcatagacat cggccagggc catgatccgc ccccccaggg gaatgttctc cccggcgagg
       61 ccctcgggat agcctgtgcc gtcccatttt tcgtggtggt acctcgtgag ggtgatgccc
      121 atcttcacca gggcatttcc cggatactcc tccaggaccc gttccagggt tccggccccc
      181 agggcggcgt gggttttcat gatttcatac tcctccgggg tcagttttcc cggcttgagg
      241 aggattctgt ccggtatgcc caccttgccg atgtcatgga ggggcgcggc gtggaaaatg
      301 ttctccacga actcgtcggt cacggtctcc cggaaccggg gatcctccct cagcgtgagg
      361 gctatcagtc tgcagtatgt ccgtgtccgc tcgatgtgct ttcccgttgc gtcgtcccgg
      421 gactccgcca gcttcgagag ggcgagaatg gtggcaagct gggattccga tatctccctg
      481 accttttccc ggaccaggtc ctcgaggtaa tggttgtgcc gttccagctc cttcctcatg
      541 cggacgaggg taagatgggt ccttacccgg gctgcgagtt cttcttcctg aaagggtttg
      601 gtgacgtaat ccactccccc tgccgcgaag gcccgaacct tgcctgcagg atcgtccagg
      661 gcgctgatga agagcacggg cacttccctg agcctttcgt cctccctgag cctccggcag
      721 atttcaaaac catccatgtc cggcatcatg atgtcgagca aaaccaggtc cggcgcctcg
      781 agagctgcgg ctttcagggc catgcctccc ctggggaagg ccgcgacgcg gtagcctctc
      841 cgtccgagca gctcgacaag gatggtgagg ttggtgggcg tgtcgtccac caccatgatc
      901 gtttcgtttc ccgtccggtc cggagccgtg ctatccatgg aacgcatcct cctccatgcc
      961 gagaatccgg gcaagctgct cgtaatcaaa ggcggccacg agatcccgga gcctcttccc
     1021 ggctgtttcg tcactggcgg ccgcttcggc ggcaagttcc cggagcctga gtatgtctcc
     1081 ccgttccagc gcccggacca ttccggagcg tattccttcc ggcaggaccg acgggaagtc
     1141 acctccggga accggagtcg attccgcgga tatcttctct cccggaagtt cccgcggaac
     1201 cgccccgatt tctttcgaga cgagggaatc ccggcccggc gttccttccc ggtcatgatc
     1261 cgtcagcacc ggaa
//
LOCUS       DAOTHD010000779         1272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_128147_length_1272_cov_4.071487, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000779 DAOTHD010000000
VERSION     DAOTHD010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128147_length_1272_cov_4.071487"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1272
                     /locus_tag="P5219_11320"
     CDS             <1..>1272
                     /locus_tag="P5219_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRX27399.1"
                     /translation="SRILSRTGADPSALAGRIEQDLTRRARVTGPGVEPGKIRISPRL
                     SRLLLKAEERADRLKDEYVSVEHLFASFFDEGRTTAAGRILAEGGVTEEKFLAALTEV
                     RGRTRVCSDNPESTYEALEKYGRDLVAMARSGKLDPVIGRDEEVRRVIRILSRKTKNN
                     PVLIGVPGVGKTAIVEGLAGRIVNGDVPEGLRDRSIFALDMGALVAGAKFRGEFEERL
                     KAVLNEVKESDGRIILFIDELHTIVGAGAAEGAIDAGNMLKPMLARGELHCIGATTVD
                     EYRKHIEKDAALARRFQPVLVEQPSVEDAVSILRGLKERFQVHHGVKIRDNALVAAAV
                     LSNRYITERFLPDKAIDLVDEACAMIRTEIDSLPAELDTVSRKVMQLEIEEAALTKEK
                     DAASLGRLKNLRAELQEAREEQAALRAGYEAE"
BASE COUNT          254 a          380 c          459 g          179 t
ORIGIN      
        1 tcccggattc tctccaggac gggggcggac ccttcggctc ttgcgggcag gatcgaacag
       61 gacctcaccc ggcgggcgag agtcaccggg cccggcgttg agcccgggaa aatacggatt
      121 tccccccgcc tttccaggct tctcctgaag gcggaggaac gggcggaccg gctgaaggac
      181 gaatacgttt ccgtggaaca tctcttcgca tccttcttcg acgaggggcg gaccacagcg
      241 gcaggccgga tcctggccga gggcggggtc accgaggaga aattcctggc ggccctcacg
      301 gaggtgcggg gccggacgcg ggtatgcagc gacaatcccg agtccaccta cgaagccctg
      361 gagaagtacg gccgggacct ggtggccatg gcccggtcgg ggaagctgga tcccgtgatc
      421 gggagagacg aggaggtacg gagggtcatc cggatcctca gcaggaagac gaagaacaac
      481 cccgtgctca tcggcgtccc cggagtcggg aagacggcca tcgtggaggg gctcgccggg
      541 cggatcgtga acggcgacgt acccgaaggt cttcgggacc ggtccatctt cgccctggac
      601 atgggggcgc tggtggccgg cgccaagttc aggggcgagt ttgaggaacg gctgaaggcg
      661 gtcctcaacg aggtgaagga gagcgacgga aggatcattc tcttcatcga cgagctccac
      721 accatcgtgg gggccggggc ggccgaggga gccatcgacg cggggaacat gctcaagccc
      781 atgctcgcca ggggagagct tcactgcatc ggcgccacca cggtggatga gtacaggaag
      841 cacatcgaaa aggacgccgc cctcgcgagg aggttccagc ccgtgctggt ggaacagcct
      901 tccgtggagg acgccgtctc catcctgagg ggcctgaagg agcgcttcca ggtccaccac
      961 ggggtgaaga tccgggacaa cgccctcgtg gcggcggcgg tgctgtcgaa caggtacatc
     1021 acggaacggt tccttccgga caaggccatc gacctggtgg acgaggcctg cgccatgatc
     1081 cgcacggaaa ttgactccct tccggcggag ctcgacaccg tctcgagaaa ggtcatgcag
     1141 ctcgagatcg aggaggcggc cctgacgaag gaaaaggacg ccgccagtct cggacggctg
     1201 aagaacctcc gggcggagct ccaggaggcc cgggaggaac aggcagccct ccgggcgggc
     1261 tacgaagcgg aa
//
LOCUS       DAOTHD010000780         1271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_128368_length_1271_cov_2.392270, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000780 DAOTHD010000000
VERSION     DAOTHD010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128368_length_1271_cov_2.392270"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="P5219_11325"
     CDS             <1..1002
                     /locus_tag="P5219_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRX27400.1"
                     /translation="IKGITAGAVGVLLSMVGMDPSTGELRYTFGNIYLMAGISFVTAM
                     IGLFGVSEALTQFRNAGTKSPKQNLDRIIPNWATFLKYLPLSIRSSLLGVFIGALPGT
                     GGDVAALLAYDQAKRTVRHPSRPFGTGAYEGIVAPETANKGAIGGAFIPMLTLGIPGD
                     AVTAIIIGALYIHGLKPGPMLMIETPHLFWMIVSCLSIASVALMVLGLVSVKPFARII
                     EIPKEIIMPVVIILSVIGTYAIQNSVVDIFYMIGFGIIGYFLRLYGYATGPMVLGLIL
                     GPMLDANYRRAMQGAENQLLPFLEGFVTSPISLFLTLAIVFLIVSQTSFYRRWRGLE"
     gene            1053..>1271
                     /locus_tag="P5219_11330"
     CDS             1053..>1271
                     /locus_tag="P5219_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRX27401.1"
                     /translation="MRKLGVALFAVCVLAGAAFGAYPEKNIQGYIMWGAGGAMDNVSR
                     AIVPIAQEHLGKTIILQNRTGATGAGSGS"
BASE COUNT          252 a          401 c          358 g          260 t
ORIGIN      
        1 ataaagggga tcaccgcagg agcggtgggc gttctcctga gcatggtggg catggaccca
       61 tccacggggg agctccggta taccttcggc aatatctacc tcatggcagg aatcagcttt
      121 gttacggcca tgatcggcct tttcggcgta tcggaagccc tgacccagtt caggaacgcg
      181 gggacaaagt cgccgaagca gaacctcgac aggatcatcc ccaactgggc aacttttttg
      241 aagtacctcc ccctgtccat ccggtcgtcc cttctcggcg tcttcatcgg tgcccttccc
      301 ggaaccggcg gtgatgttgc cgccctgctg gcctacgacc aggccaagcg gaccgtcagg
      361 catccctccc gtcccttcgg cacaggggcc tacgagggca tcgttgcccc cgagacggcc
      421 aacaagggag ccatcggcgg cgccttcatc cccatgctga ccctgggaat cccgggagac
      481 gccgttacgg ccatcatcat cggcgccctc tacatccacg gcctcaaacc gggccccatg
      541 ctcatgatcg agacgcccca cctcttctgg atgatcgtca gctgcctctc aatagccagc
      601 gtggccctca tggtcctcgg cctcgtgagc gtgaagccct tcgccaggat catcgagatt
      661 ccgaaggaga tcatcatgcc ggtggtgatc atcctctcgg tcatcggaac ctatgccatc
      721 cagaacagcg tggtggacat cttttacatg atcggcttcg gtatcatcgg gtattttctg
      781 aggctctacg gctatgccac gggccccatg gtcctagggc tcatactggg tcccatgctc
      841 gacgccaact acagaagagc catgcaggga gcggagaacc agcttctgcc cttcctcgaa
      901 ggcttcgtca ccagtcccat cagcctcttc ctgacgctgg ccatcgtctt tctgatcgtg
      961 tcccagacgt ctttctacag gagatggaga ggcctggaat agttttcccc tgccggggaa
     1021 gagagaaacg aaaaataagg gaggaatgtt tcatgagaaa actcggtgtt gcgctgtttg
     1081 cggtatgtgt tctggctggg gctgccttcg gtgcctaccc ggaaaagaac atccaggggt
     1141 acatcatgtg gggcgccgga ggcgccatgg acaacgtgtc ccgggccatc gttcccatcg
     1201 cccaggaaca tctcggaaag accatcatcc tgcagaaccg caccggtgcc acgggagccg
     1261 gcagcggcag c
//
LOCUS       DAOTHD010000781         1270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_128459_length_1270_cov_3.655144, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000781 DAOTHD010000000
VERSION     DAOTHD010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128459_length_1270_cov_3.655144"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..496)
                     /locus_tag="P5219_11335"
     CDS             complement(143..496)
                     /locus_tag="P5219_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27402.1"
                     /translation="MLNRVEAAVPSGEFGEIEEEIRRFSGELDAALEKKRKECRLARE
                     QALLRLSEMEAETLRQVEAEWRAREKELELLASSLEKEISSIRDEISGRISGDPVLKS
                     LREEVFAALLGEKEP"
     gene            complement(480..953)
                     /locus_tag="P5219_11340"
     CDS             complement(480..953)
                     /locus_tag="P5219_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRX27403.1"
                     /translation="MKTAAKDIMNFDLTSVLEDDLVQDAIHVLYSHNLQGIPVLDEDW
                     NLVGFLSESDVLKAAVPTYLEVLARSSFLEDEEDLLLSRFRSFGNKKVGDFMNRNPIF
                     VEPETSLMAVADLMIRKSVKRLPVVENGKLAGMISREAFCEFLMDESVSRGAEQG"
     gene            complement(950..>1270)
                     /locus_tag="P5219_11345"
     CDS             complement(950..>1270)
                     /locus_tag="P5219_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HRX27404.1"
                     /translation="AGAVRDSGAKILFVAMGVPRQEIWIRENARLLGDMVAVGVGGAF
                     DVVSGQLRRAPGAWQKLGLEWLFRLLQEPWRWKRDIDLFIFVCRVFLTKLGLRPFRED
                     RSAE"
BASE COUNT          270 a          403 c          319 g          278 t
ORIGIN      
        1 agcgtattca gccgtgcaga ttcgccggct gaaggaccgc ttccctgttc gagatggagc
       61 accccgagcc tgtgaagctc ctctatgatt tctctccgct tgccggtgac cccccagagg
      121 gccagccgga tcattttccg aatcatggtt ccttctctcc aagaagggcg gcgaacactt
      181 cttcccgaag gcttttcagg accgggtcgc ctgaaatccg ccctgaaatt tcatccctga
      241 tggacgaaat ttccttctcc aggctggacg cgaggagttc aagctctttt tccctggccc
      301 gccattcggc ctccacctgt ctcagcgtct cggcctccat ctcggaaagc cgaaggagtg
      361 cctgctctct ggcgagacgg cactccttcc tttttttttc aagggcagca tccagttcgc
      421 ctgagaaacg gcgaatttct tcctctattt cgccgaattc acctgacgga accgcagctt
      481 caaccctgtt cagcaccgcg gctcacgctt tcatccatga gaaactcgca gaaagcctcc
      541 cggctgatca tacctgcgag tttgccgttt tcaacaacgg gaagccgctt caccgacttg
      601 cggatcatca ggtccgccac cgccatgagg cttgtctccg gttccacgaa gatggggttc
      661 cggttcatga aatcccccac cttcttgttt ccgaaagacc ggaatctcga aagaagaaga
      721 tcctcttcgt cctccaggaa agaagaacgg gcaagcactt caagataggt gggaactgcg
      781 gctttcagga cgtcgctttc ggaaaggaat cccacaaggt tccagtcttc gtcgagaacg
      841 ggaatcccct gcaggttatg gctgtacaga acgtggatgg catcctgaac caggtcatcc
      901 tccaggaccg aggtgaggtc gaagttcatt atgtccttcg cggcggtctt cactctgcgc
      961 tcctgtcttc cctgaaggga cgcaggccga gcttcgtgag aaaaaccctg cacacaaaga
     1021 tgaaaagatc gatatcccgc ttccacctcc agggttcctg aagaaggcgg aacagccatt
     1081 caagaccgag tttctgccag gcgcccggtg cccttctgag ctgacccgac accacgtcaa
     1141 aggctccgcc gacccccacg gcgaccatat cgccgagaag ccgggcgttc tcccggatcc
     1201 agatctcctg acggggaaca cccattgcaa cgaaaagaat cttcgcaccg ctgtctcgga
     1261 cggctccggc
//
LOCUS       DAOTHD010000782         1269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_128636_length_1269_cov_3.051895, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000782 DAOTHD010000000
VERSION     DAOTHD010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128636_length_1269_cov_3.051895"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1269)
                     /locus_tag="P5219_11350"
     CDS             complement(<1..>1269)
                     /locus_tag="P5219_11350"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRX27405.1"
                     /translation="LFLRLASRPRLRVTASASFSEAGVSLEEIPPFRGNMDKFRWMGD
                     SWKKDGYSLFLATKNRRILSFGEEASVFPVDGTLSKGFLDPVSRIAYISDLELAGISE
                     KITGGGMLSAPPREWSDRLSEGQLLVHEDYGLCVFRGSEEVSVAGEMMDSLILEFDGS
                     QRLFLPVLQLHKITPLPDHMSGDVRLDSLRGVKWKKSVAKTRERVQQEIRGLLELYAK
                     RELVEGFAFPPPDGLYREFEEAFPHVETKDQLTASAEVAADMESPYPMDRLLVGDVGY
                     GKTEVAVRAAFKAVQGGKQAAVLVPTTILAQQHYMSFTARMTGFPVNVALLSRFSSKK
                     EQRTVLEETASGRVDILIGTARMLQKDVAFKDLGLIVVDEEHRFGVLSKEKLKEAREN
                     VDVLMLSATPIPRTLALSLKGMRSISLLTE"
BASE COUNT          231 a          387 c          418 g          233 t
ORIGIN      
        1 gctcggtgag cagggagatg ctcctcatac ccttcaggga gagggcgagg gtccggggga
       61 tgggggtggc ggagagcatg agcacgtcca cgttctcccg ggcttccttg agcttttcct
      121 ttgacagcac gccgaagcgg tgctcctcgt ccaccacgat aaggccgaga tccttgaagg
      181 ccacgtcctt ctggagcatc cgtgccgtgc cgatgagtat gtccaccctg ccagaagcgg
      241 tttcctccag caccgtgcgc tgctcttttt ttgacgagaa tctggagaga agggccacgt
      301 tcaccgggaa ccccgtcatg cgggcggtga aggacatgta gtgctgctgg gcgaggatgg
      361 tggtaggcac gagcacagcg gcctgcttcc ctccctggac ggccttgaag gcggcccgga
      421 cggccacttc ggtcttgccg tagcccacgt cgcccacgag aagccggtcc atgggatagg
      481 ggctttccat gtcggcggcc acctcggccg aggcggtgag ctggtctttg gtctccacgt
      541 gggggaaggc ctcttcgaac tcccggtaca ggccgtcggg aggcggaaag gcgaacccct
      601 ccaccagctc ccgcttcgcg taaagctcca gcagcccacg gatctcctgc tgcacccgct
      661 ccctggtctt ggccacgctc tttttccact tgacgccccg gagggagtcg aggcggacgt
      721 cgccgctcat atggtccggc aggggagtga tcttgtggag ctggaggacg ggaaggaaga
      781 gccgctggct gccgtcgaac tcgaggatca ggctgtccat catctccccg gcgacggaca
      841 cttcctcgga gccccggaag acacagaggc cgtaatcctc gtggaccagg agctgccctt
      901 cggaaagccg gtcgctccat tcccggggag gagcggagag cattcctcca ccggttatct
      961 tctccgatat ccccgccagc tcaaggtcgg aaatataggc gatgcgggat acgggatcga
     1021 ggaagccctt cgagagggtg ccgtccacgg gaaagacgga agcctcctcg ccgaaggaga
     1081 gaatgcgccg gttcttcgtg gcgaggaaga gagaataacc gtctttcttc caggagtcgc
     1141 ccatccaccg gaacttgtcc atgttgcccc ggaagggggg tatctcttcc aggctcaccc
     1201 cggcctcgga gaaggaggcc gacgcggtga ccctgagcct gggtctcgat gcgagacgga
     1261 gaaagagtt
//
LOCUS       DAOTHD010000783         1268 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_128783_length_1268_cov_3.160758, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000783 DAOTHD010000000
VERSION     DAOTHD010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128783_length_1268_cov_3.160758"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1241
                     /locus_tag="P5219_11355"
     CDS             <1..1241
                     /locus_tag="P5219_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRX27406.1"
                     /translation="AVAYRTKHGLGNDRMGIILMRVAGKWRGRYYYPTMAGVGFSRYY
                     RRWTTRIRSEDGIIRLVFGMGTTSTKRGYARTFALTLPCLRPEGQNPFNIMRHAQEHF
                     QVIDREQKSLVTVDVKRIWKDIFPYHSCFPDYAQIYSPDDDGGYFTPMSRHGSSIKTG
                     EKVCFTFEEFACRSKHFFDRMKKLLALLDREMGVPADVEFAYHPSEDLVEIVQARPLW
                     LSEQTHSVTIPDLENRKVVLKADRMVTHGVKENIPYLVYVDHHVYAQETRFHDVARGI
                     GTVNDTLKGSRFILVAPGRVGSSNPLLGVPVQYNEITRCSCIVEVGFPKEGYMPELSF
                     GTHFFTDLEIDGILYMPVYEGAKNNIFDESFFDSSPYALGSHAGIRIYSGSFSVYTDG
                     DRNFGVVVADRVDEPENGWD"
BASE COUNT          282 a          376 c          374 g          236 t
ORIGIN      
        1 tggcggtggc ctaccggaca aagcacggcc ttgggaacga ccgcatgggg atcattctta
       61 tgcgggtcgc aggcaaatgg cggggcaggt attactaccc caccatggca ggagtgggtt
      121 tctcgagata ttacaggaga tggaccacca ggatccggtc agaggacggg atcatccgcc
      181 ttgtgttcgg catgggcact acaagcacga agcggggata cgcccggacg ttcgccctga
      241 ccctgccgtg cctccggccg gagggacaga accccttcaa catcatgcgc cacgcccagg
      301 aacatttcca ggtgatagac cgggaacaga aatcccttgt gacggtggac gtaaagcgca
      361 tctggaagga cattttcccc taccacagct gtttccccga ttatgcccag atctacagcc
      421 ccgacgacga cggggggtac ttcacgccca tgtcccgtca cggttccagc atcaagaccg
      481 gggaaaaggt ctgctttacc ttcgaggagt ttgcctgccg gtcgaagcat ttcttcgaca
      541 ggatgaagaa gcttctcgcc ctcctcgaca gggagatggg ggttccagcg gacgtggagt
      601 tcgcttatca cccaagcgaa gacctggtgg agatcgtcca ggcccgaccg ctctggctga
      661 gcgagcagac ccactccgta accataccgg acctcgagaa cagaaaggtg gttctcaagg
      721 cggacagaat ggtgacccac ggagtgaagg aaaacatccc ctacctggtg tacgtggacc
      781 atcacgtcta cgcccaggaa acccgcttcc acgacgttgc ccggggcata ggcacggtga
      841 acgacaccct gaagggctcc cggttcattc tcgtggcgcc cgggagggtg ggttccagca
      901 accccctgct cggcgtgccg gtacagtaca acgagatcac gagatgctcc tgcatcgtgg
      961 aggtgggatt ccccaaggag ggatacatgc cggagctttc cttcggcacc cacttcttca
     1021 ccgacctgga gatcgacgga atcctctata tgccggtcta cgaaggggcg aagaacaaca
     1081 ttttcgatga aagcttcttc gactcctccc cttacgccct ggggtcccat gccggaatcc
     1141 gcatctactc cgggtccttc tcggtgtaca ccgacggcga ccggaatttc ggcgtggtgg
     1201 tagctgacag ggtggacgaa ccggagaacg ggtgggacta aaacccggcc tttgaaatac
     1261 aaagacgc
//
LOCUS       DAOTHD010000784         1268 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_128808_length_1268_cov_2.847486, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000784 DAOTHD010000000
VERSION     DAOTHD010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128808_length_1268_cov_2.847486"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="P5219_11360"
     CDS             <1..678
                     /locus_tag="P5219_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRX27407.1"
                     /translation="GVYLSIGVMEREGGTLFCTLLYFGPDGSLLAKHRKLKPTGSERL
                     IWGEGDGSTLAAVNTPYGVMSGLICWENYMPLARAAIYGRNPSIYLAPTADQREGWQC
                     SMRHIALEGRCFVLSCNQFVTKDIYPEDLQCFQDLESQPDILCRGGSAIIDPLGNYLA
                     GPSWDREEILCADLDLAAVVEARYDFDVMGHYARPDVFTLLVNGEPLRGIDYSGDPFL
                     PGEALTE"
     gene            675..>1268
                     /locus_tag="P5219_11365"
     CDS             675..>1268
                     /locus_tag="P5219_11365"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HRX27408.1"
                     /translation="MSGTHSDGKGTPRNGLPGTPFCDGPLLRETAERAIRWLEDLPSR
                     RVGTWTSAEELRKTLGGTLNDEGMAPPDVVAAFSEGAAPGLTGSPGPRYFGFVTGGAL
                     PASLAADWLASAWDQNAVLAVSSPAASAAEEIVSEWILDLLGLPPESSVGFVTGCQMA
                     NFTCLAAARHEVLRRSGHDVEKDGLQGAPRVTVIAGEE"
BASE COUNT          208 a          441 c          416 g          203 t
ORIGIN      
        1 ggggtctacc tgtccatcgg cgtgatggag cgggaagggg gaacactctt ctgcaccctg
       61 ctgtatttcg gccccgacgg ctccctcctg gcgaaacacc ggaaactgaa gcccacgggg
      121 agcgagcggc tcatctgggg agagggggac ggcagcaccc tcgccgcggt gaacaccccc
      181 tacggcgtca tgagcggtct catctgctgg gaaaactaca tgcccctcgc ccgggccgcc
      241 atatacggcc ggaacccctc catatacctc gccccgacgg ccgaccagag ggaggggtgg
      301 cagtgctcca tgcgccacat cgccctggag ggacggtgct tcgtcctgtc ctgcaaccag
      361 ttcgtcacga aggacatcta ccccgaggat ctccagtgct tccaggatct tgaatcccag
      421 ccggatatcc tgtgccgggg gggcagcgcc atcatcgacc ccctcgggaa ctacctcgca
      481 gggccgtcat gggaccggga ggagatcctc tgcgccgacc tcgacctcgc cgcggtggtg
      541 gaagcccgct acgatttcga cgtcatgggg cactatgccc gccccgacgt gttcactctc
      601 ctggtaaacg gagagcccct gcggggaatc gactattccg gggatccctt tctgccgggg
      661 gaggctctga ccgaatgagc gggacccatt ccgacggaaa gggcacgccc cgaaacggcc
      721 tgccggggac gcctttctgc gacggtccgc tgctgcggga aacggcggag agggcgatcc
      781 gctggcttga agaccttccg tccagaaggg tcggcacctg gacgtccgct gaggagctca
      841 ggaagaccct cggcggaacg ctgaacgacg agggaatggc ccccccggac gtggtggccg
      901 ccttttcgga aggcgccgcc cccggcctca ccggttcgcc ggggccgcgg tatttcggct
      961 tcgtcaccgg gggagccctt ccggcgagcc tcgccgcgga ctggcttgcc tccgcctggg
     1021 accagaacgc cgtcctcgcc gtctcctctc ccgccgcaag cgctgcggag gagatcgtct
     1081 cggaatggat cctcgacctc ctcggcctgc cgccggaatc ctccgtcggc ttcgtcacag
     1141 ggtgccagat ggccaacttc acctgcctgg cggccgcacg gcacgaggtc ctcaggcgct
     1201 ccggccatga cgtggagaag gacggcctcc agggagctcc ccgggtgacc gtcatcgccg
     1261 gcgaggag
//
LOCUS       DAOTHD010000785         1268 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_128855_length_1268_cov_2.206925, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000785 DAOTHD010000000
VERSION     DAOTHD010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128855_length_1268_cov_2.206925"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="P5219_11370"
     CDS             <1..352
                     /locus_tag="P5219_11370"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HRX27409.1"
                     /translation="MGAKVTSLERIAPLADRARERLDGLGFDVRVVWSDGSPESRPAG
                     SFDGVIVTAAAPEMEDWWSGVLAPGGRLIVPLSVMSGGQRLLLRKKAAGGEYEDTWHD
                     YCRFVPLLKGRKSG"
     gene            390..875
                     /locus_tag="P5219_11375"
     CDS             390..875
                     /locus_tag="P5219_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HRX27410.1"
                     /translation="MKDLLAPRSLAVSGLVAAAYAVVTILFAPISYGHIQVRISEALT
                     VLPFLFPQAVPGLFIGCLIANFAGGFGILDVVLGSGATLTAAVLTARMPNVWLASVPP
                     VVVNMAVVGGYLSFLLDIPFFLCAFYVGLGQVIACCFLGIPLVLLLERRMKKGGTGPA
                     L"
     gene            complement(879..>1268)
                     /locus_tag="P5219_11380"
     CDS             complement(879..>1268)
                     /locus_tag="P5219_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRX27411.1"
                     /translation="ETAALFSALEEKFRAVMDDDFNTAAATGVLFDGVKTVNTYLKES
                     DLPDPAVLEAAETFFRKLDSVLGILPPEASGEEGESAGIEKLIAERNEARKRKDFSRS
                     DEIRDELAARGIVLEDTPDGTKWKKKG"
BASE COUNT          190 a          366 c          410 g          302 t
ORIGIN      
        1 catgggcgcg aaggtgacga gcctggagcg gatcgccccc cttgcggaca gggcccggga
       61 acgccttgac gggctcgggt tcgacgtccg ggtggtctgg tccgacggca gtccggaaag
      121 ccggcctgca ggctccttcg acggagtgat cgttaccgct gcggccccgg agatggagga
      181 ttggtggtcc ggcgttctgg ctcccggagg gcggctgatt gttccccttt cggtgatgtc
      241 ggggggccag cggcttcttc tgcggaaaaa ggccgccggt ggagaatacg aggatacatg
      301 gcacgattac tgccgtttcg ttccgctgct gaaggggcgg aagagcgggt gaaccggagg
      361 aagggcttat gaggagagca ccgcagaata tgaaggatct tcttgccccg aggtcactgg
      421 ccgtttcggg gctggtggcg gccgcctatg ccgtggtcac catactgttc gcccccattt
      481 cctacggcca tatccaggtg cggatctcgg aagccctcac ggtgcttccc tttctgtttc
      541 cccaggcggt tccgggtctc ttcatcggct gtcttatcgc caatttcgcg ggaggcttcg
      601 gcatcctgga cgtggtgctc gggagcgggg cgaccctgac cgctgcggtt ctcacggcga
      661 ggatgcccaa cgtctggctt gcctccgttc ctcccgtggt ggtgaacatg gcggtggtgg
      721 gtgggtacct ctcttttctc ctggatattc cttttttcct ctgcgccttc tatgtggggc
      781 tggggcaggt cattgcctgc tgcttccttg gcattccgct ggtacttctc ctcgaacgga
      841 gaatgaaaaa gggcgggacc ggtcccgccc tgtaaagact atcccttttt cttccacttg
      901 gttccgtcgg gggtgtcctc aaggacaatt cccctggcgg cgagttcgtc ccgtatctcg
      961 tcggaccgac tgaaatcctt tctctttctt gcttcgttcc tctccgcgat gagcttttca
     1021 attccggcgg attctccttc ttctcccgac gcttcggggg gaaggatacc cagaacggaa
     1081 tcgagcttcc tgaagaaggt ttccgcagcc tcaagcacgg cagggtccgg gagatcgctc
     1141 tccttgaggt aggtgttgac ggtcttgacg ccgtcgaaaa ggacgccggt ggccgctgcg
     1201 gtgttgaagt cgtcatccat gacggcccgg aatttctcct cgagagcgga aaagagggcg
     1261 gccgtttc
//
LOCUS       DAOTHD010000786         1264 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_129457_length_1264_cov_2.119934, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000786 DAOTHD010000000
VERSION     DAOTHD010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129457_length_1264_cov_2.119934"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="P5219_11385"
     CDS             <1..877
                     /locus_tag="P5219_11385"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HRX27412.1"
                     /translation="SIKFHAAKKKADGSLETVLDQNDIARLGEGLRETGLIAPEALER
                     NALSVAAFAEKAKELGAVPEIVGTMALRTARNADAFVKRVKELTGLDLKVIPGEEEAR
                     LSYLAVLSGLPLDGGDLVTFDTGGGSTEFVYGRGAEMLRKFSVPLGAVRITEEFFVDD
                     PVRPGSVDAAMEEIRKSLTGGGVLGSPEVIVGMGGTVTSLASVKFKMETYDPDVVQGS
                     VLTLAELKEMAAMFASMTLEERKGVTGLQPKRADVILAGTCIVCAILELLGASSFTVS
                     DRGLRHGLAYELFRK"
     gene            945..>1264
                     /locus_tag="P5219_11390"
     CDS             945..>1264
                     /locus_tag="P5219_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRX27413.1"
                     /translation="MKRIIAVLCIASLAVLGASAAFAAYPEKPIKVVNYVGPGGLMDV
                     TSRKFVSIAKKFTDATFVVENKQGAGGLVGMQAVLEQPADGYTVFAATTSNIAKVLTS
                     KKNV"
BASE COUNT          259 a          353 c          422 g          230 t
ORIGIN      
        1 ttccatcaag ttccatgcgg cgaagaaaaa agcggacggg tcccttgaga ccgtgctgga
       61 ccagaatgac attgcccgcc tcggcgaggg acttcgggag acggggctga tcgctccgga
      121 agcccttgaa cgaaacgccc tgtccgtggc ggctttcgcg gaaaaagcga aggaactggg
      181 agccgtcccg gagatcgtgg ggaccatggc cctgaggaca gccagaaacg ccgatgcttt
      241 cgtgaaacgg gtgaaggagc ttacgggact cgacctgaag gtcatccccg gcgaggaaga
      301 ggcgaggctg tcctatctcg ccgtcctttc cggccttccc ctcgacggag gagacctcgt
      361 caccttcgat acgggcgggg gaagcaccga gttcgtctac ggcagggggg cggaaatgct
      421 ccggaagttc agcgttcccc tgggggcggt gcgcatcacc gaggaattct tcgtcgacga
      481 tcctgtccgt ccgggatcgg tggacgccgc catggaggag atacggaaga gcctcaccgg
      541 cggaggggta ctcggctccc ccgaggtgat cgtgggcatg ggaggaacgg tcaccagtct
      601 tgcgtccgtc aagttcaaaa tggagaccta cgaccccgac gtggtccagg ggtcggttct
      661 caccctggcc gaactgaagg agatggccgc catgttcgcc tccatgacgc tggaggagcg
      721 gaaaggggtg acgggattgc agccgaagcg ggctgatgtt atccttgccg gaacatgcat
      781 tgtctgcgcc atccttgagc tgctcggggc ctcctcgttc accgtgagcg acaggggcct
      841 gcgccacggg ctggcttacg agcttttccg caaatagggg aatccgcccg ggagaacaga
      901 atccgggcag gggaattcca aaaattctca ggaggtgttt tttcatgaaa cggatcatcg
      961 ccgttctgtg catcgcgagt ctcgctgtgc tcggggcctc ggccgccttc gcggcctacc
     1021 ctgaaaagcc catcaaggtc gtgaactacg tggggccggg aggcctcatg gacgtgacca
     1081 gcaggaaatt cgtctccata gcgaagaaat ttacggatgc gacctttgtc gttgaaaaca
     1141 agcagggggc gggcgggctg gttgggatgc aggcggtgct tgagcagcct gccgacggat
     1201 acaccgtgtt tgcggcaacc acgtccaaca tcgccaaggt gctgacctcc aagaaaaacg
     1261 tgga
//
LOCUS       DAOTHD010000787         1263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_129577_length_1263_cov_2.393212, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000787 DAOTHD010000000
VERSION     DAOTHD010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129577_length_1263_cov_2.393212"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(494..>1263)
                     /locus_tag="P5219_11395"
     CDS             complement(494..>1263)
                     /locus_tag="P5219_11395"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRX27414.1"
                     /translation="SAPGAGDYLKAMFTVVPLTDYLVRSGLDRFFMILPLAAAALSFS
                     LALRLRPARFQGSPGQDQPMEWGTYGDLFRDTPAGKIVFSCFFFGLCDASIVFIGTVL
                     LEKGLVPSWFIFSLGAGALFVRTAGRNLFNRYQRTVFAGPSFLVMALCLLGLAFADSS
                     LGLVFWGFFYGMGMGYGYPAHLALTADLAAPRLRAKASALVHFSMDLSWFILPLYMGF
                     GCTLLGTRQAFLLFTLICIATAVMTTVMWLPGKKGRV"
BASE COUNT          283 a          351 c          427 g          202 t
ORIGIN      
        1 ctgcccatga accttcccta cacaatgacg ccggagatgg cggcggacgc cgccctctcc
       61 ttccggccca aaatcctcta cccctatcac tacgggcaga cggatccgga aaagcttgtg
      121 gacctcctga aggactcggg gattgaagtt cgggtcagaa agatgagcta gggagagccg
      181 gaacggcctt tgcctcccgt caacccgggg cggcattgct gtttctgtca tcctgaggag
      241 cgaacgcgac gaaggattcc ctttggtccg ggatcgtaaa atttcgaggt ccttcgctcc
      301 gctcaggacg acagaggaag agtgtcatcc tgagaccggg tttggggttt gtcatcctga
      361 ggagcgacag cgacgaagga tcttttttgt tccggggcct caatggcgaa atcctcccca
      421 cgctgcgctc gggatgacaa aatagaggaa tcaggaagaa aaaggtggcc gggacattgt
      481 gtcccggctt ttttcatacc ctcccttttt ttccgggcag ccacatgacc gtggtcatca
      541 ccgccgtggc gatgcagatc agggtaaaga gaagaaaagc ctgccgggtt cccaacaggg
      601 tgcagccgaa ccccatgtag aggggaagga tgaaccagct caggtccatg gagaagtgga
      661 ccagcgccga ggccttcgcc cggagacggg gcgcggcgag atccgccgtg agggcgagat
      721 gggcggggta tccgtatccc atgcccatgc cgtagaaaaa tccccagaag accagcccga
      781 gggaagagtc ggcgaaggcg aggccgagaa ggcagagggc catgacgagg aaggaggggc
      841 cggcgaagac ggtcctctgg taccggttga agaggtttct tcccgccgtc cggacgaaga
      901 gggcccccgc cccgagggag aagatgaacc acgaggggac gagccccttt tccagaagca
      961 ccgtgccgat gaagacgatg gatgcgtcgc acaggccgaa gaagaagcat gagaagacga
     1021 ttttccccgc aggcgtgtcc cggaagagat cgccgtaggt cccccattcc atgggctggt
     1081 cctgtcccgg ggagccctgg aaccgggcgg gcctgagccg gagggccagg gagaaggaga
     1141 gggccgccgc cgccagggga agtatcatga agaaccggtc caggccgctc cggacaaggt
     1201 agtcggtgag ggggaccacg gtgaacatgg ccttgaggta gtcgccggcc ccgggggcag
     1261 aga
//
LOCUS       DAOTHD010000788         1260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_129974_length_1260_cov_2.864730, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000788 DAOTHD010000000
VERSION     DAOTHD010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129974_length_1260_cov_2.864730"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="P5219_11400"
     CDS             complement(<1..211)
                     /locus_tag="P5219_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRX27415.1"
                     /translation="MARQFVHLHVHTEYSLLDGAIRCDRLAARAVEYGMPAVAMTDHG
                     AMYGAVEFYDKCLSAGIKPIIGCEVY"
     gene            complement(275..>1260)
                     /locus_tag="P5219_11405"
     CDS             complement(275..>1260)
                     /locus_tag="P5219_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27416.1"
                     /translation="KDVLSSLSLSAAEASFLEEEGPKWPTGELSGMMLFCSRLIPQVR
                     TGLRSDVLSGLLAARILESLQPHDEEKAVPSGGEGEHRSRHPEPAKKTVPVPSAPVGD
                     PSINTAPSERFRRKNPETASEKENPVAEAPSRSHEPVPLLPENWPGFEKVLFARDLLL
                     YSTLAGTSVSLENRTISIHFPEDSPYCFEVLSIERNAFSLASRASEYFGEDVSVILKM
                     GSREKKCAGGGNGAGDDTEWQNPGPTIPLFRIPRDEDESPREESGTAPAALSPGRPLP
                     PVSTGGGPDDPAVPFEGLVNEVLKWGGGEVVLVKREDREGDLPDEIVPPGE"
BASE COUNT          259 a          349 c          384 g          268 t
ORIGIN      
        1 cgtagacctc gcagcctatg atgggcttga tgcccgcgga aaggcattta tcgtaaaatt
       61 ccacggcccc gtacatggct ccgtggtcgg tcatggcaac ggcgggcatt ccatactcga
      121 cggccctggc ggcgaggcgg tcgcaccgga tggcgccgtc gagaaggctg tattcagtat
      181 ggacgtgaag atgaacaaat tgtcgggcca ttgttgacgc tccttgtggg aagatcggta
      241 agaagaacgg gttctgcggc gcctcggaga gaggtcactc tcccggagga acgatttcgt
      301 cgggaaggtc tccttccctg tcctctctct tcacgaggac gacttctccg cctccccatt
      361 tcaggacttc attcaccagc ccttcaaagg gaacagccgg gtcatccggt ccacctcccg
      421 ttgataccgg aggaaggggt ctgcccggag acaaggctgc cggtgccgtt ccgctctcct
      481 cccggggaga ttcatcttca tcccggggaa ttcgaaaaag ggggattgtc gggccgggat
      541 tctgccattc agtatcgtca ccggctccgt ttcctcctcc cgcacatttt ttctcgcggc
      601 tgcccatctt cagaatgacg gaaacatcct cgccgaagta ttcagaagct cttgatgcga
      661 gggaaaaggc gttgcgctct atggagagca cctcaaagca atagggcgaa tcctccggaa
      721 aatgaatgga aatggtgcgg ttttccaggg aaacggaggt tccggccagg gtggagtaca
      781 acagcaggtc tctggcgaag agcactttct caaaccccgg ccagttttcc ggaagcaggg
      841 gaacaggttc atgagaccgt gatggggctt cggccacagg attctccttt tctgaggctg
      901 tttccggatt ttttctccga aaccgctcgg agggagcggt attgatggat ggatccccca
      961 cgggcgccga aggcacagga acagtctttt tggccggctc cggatgacgg gagcgatgtt
     1021 ctccttcccc tccggaaggg actgcctttt cttcatcatg gggctgcagg gattcaagaa
     1081 tccgtgcggc gagaagaccg gagaggacgt cggaccggag tccggtcctg acctggggga
     1141 tgagcctgga gcagaagagc atcatcccgg aaagctcccc ggtgggccac tttggccctt
     1201 cctcttcgag aaaggaggct tcagccgccg aaagagacag ggaggacaga acgtcctttt
//
LOCUS       DAOTHD010000789         1257 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_130448_length_1257_cov_2.664725, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000789 DAOTHD010000000
VERSION     DAOTHD010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130448_length_1257_cov_2.664725"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="P5219_11410"
     CDS             complement(<1..234)
                     /locus_tag="P5219_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27417.1"
                     /translation="MDKDVWSWKKRNAVVLNVSVETTPAGLAVLLYFRPSPFESPSLP
                     KLPDKKETPGRKSATVGIIPKRKERFFFGIMPTV"
     gene            259..702
                     /locus_tag="P5219_11415"
     CDS             259..702
                     /locus_tag="P5219_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRX27418.1"
                     /translation="MAEERKAETSPLAPGEVVTCTVEQIMPYGAFVRLKTGQRAMIHI
                     SELSHSFVKKVEDILSLSQEVQAKVIKIDEKGRIDLSLKKMEAPRPAQSPRASSFGPA
                     APEDTRDSFEKKLSNYLKASEAKIADLNNKLNSSRAAKKRGKPAK"
     gene            699..>1257
                     /locus_tag="P5219_11420"
     CDS             699..>1257
                     /locus_tag="P5219_11420"
                     /inference="COORDINATES: protein motif:HMM:NF016314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HRX27419.1"
                     /translation="MNRSAGKLSGGRIRPRSFFEPSGPRDLRIVSLQMKGRRFDRAVV
                     AGIARRCMFGVPQVIACSPLRDGKPFPTTFWLTCPHLVRLCGVLESEGAVTELERTRL
                     EQPVPWTEYNLFHARLRLLMVRPASARFLRKHRGGVWSTLSRGGTGGIDYLSPSGGGV
                     KCLHLQAASWLALGFHPAGEYLRKAL"
BASE COUNT          266 a          383 c          370 g          238 t
ORIGIN      
        1 gactgtaggc attataccaa agaagaagcg ttcctttctc tttggtataa tgcctacagt
       61 cgcgcttttt cttcccgggg tctccttctt gtccggcagc ttcggcaggg agggggactc
      121 gaatggagat ggacggaaat acagaagcac agctaaacct gcgggggtgg tttctaccga
      181 aacattcagt accacggcgt ttcttttttt ccaggaccat acatccttat ccaatactcg
      241 tggagggtag aaaaccgcat ggcagaagaa agaaaagcag aaacctcccc cctcgctccc
      301 ggggaagttg tgacctgtac ggtggaacag atcatgccct acggcgcctt tgtccggctc
      361 aaaacgggcc agagggccat gatccacatc tcggagctgt cccacagctt cgtgaagaag
      421 gtcgaggaca ttctctccct cagccaggaa gtccaggcga aggtcatcaa gatcgacgaa
      481 aaaggcagga tcgacctttc cctcaagaaa atggaggctc cgaggccggc ccagtccccc
      541 agggcatcat ccttcgggcc tgcggcaccc gaggacacca gggactcctt tgagaagaaa
      601 ctgtcgaact acctcaaggc aagcgaagcg aagatcgccg acctcaacaa caagctcaac
      661 agctcaaggg cggcgaagaa gcggggcaag ccggcgaagt gaaccggtca gccggaaagc
      721 tttcaggggg gcggatccgc ccccgttcct tttttgagcc ctccggtccc cgggacctcc
      781 gtatcgtctc gctccagatg aaggggcgca gattcgaccg ggctgtggtc gcaggaatcg
      841 cccggcgatg catgttcggt gtgccgcagg ttatcgcctg cagtcccctg cgggacggga
      901 agccctttcc gaccacattc tggctcacct gtccccacct cgtgaggctg tgcggggttc
      961 tcgaatcgga gggggcggtg acggaactcg aacggacaag gctggaacag cctgttccct
     1021 ggacggagta caacctcttc cacgcccggc tccgcctgct catggtgcgg cccgcctccg
     1081 cccgcttcct ccggaaacac cgcggtggcg tatggagcac cctgagccgt ggaggtacgg
     1141 gaggaattga ctacctctca ccctccgggg gaggggtgaa atgccttcac ctccaggcgg
     1201 cgtcctggct tgccctcggt tttcaccccg cgggagagta cctccggaag gctctcg
//
LOCUS       DAOTHD010000790         1257 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_130499_length_1257_cov_2.009983, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000790 DAOTHD010000000
VERSION     DAOTHD010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130499_length_1257_cov_2.009983"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /gene="cbiD"
                     /locus_tag="P5219_11425"
     CDS             complement(<1..934)
                     /gene="cbiD"
                     /locus_tag="P5219_11425"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase
                     CbiD"
                     /protein_id="HRX27420.1"
                     /translation="MDGKPFDGLGYTGGLRRGYTTGTCAAAAAKGAARMFLSGAPLSS
                     VTVTLPGGQSLTLPLEECTLSEKLARCAVRKDSGDDPDGTSGMLIVAEARPSSRPGVT
                     LSGGPGIGKVTRKGLPVPPGEWAINPGPRRMIESEVGALLLEGQGVEIAISAPEGEER
                     TKTTWNPRLGIEGGISILGTTGIVEPKSTAAYLASIDLYISSAQAFDSPRPGTVFLVP
                     GYVGEKCLAERFGAPRELIVRMGDHVGHALKECASRGAGPVHLFGHVGKWAKVAAGLF
                     NTHCDFGDARLETLAACAGAEGASKEQVRELLSLP"
     gene            complement(939..>1257)
                     /locus_tag="P5219_11430"
     CDS             complement(939..>1257)
                     /locus_tag="P5219_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HRX27421.1"
                     /translation="GEDGKRAATTRARAAMRKALPLLDGSIVAIGNAPTALFEVCDLV
                     KKGLCKPALVVGIPVGFVGAAESHDETVTLDCPWITAPGPRGGSPVAAAIVNALIRLA
                     EKE"
BASE COUNT          180 a          418 c          461 g          198 t
ORIGIN      
        1 gggggaggga gagaagctcc cggacctgtt ccttcgacgc cccttcggcc ccggcgcatg
       61 cggccagggt ctcgaggcgg gcgtcgccga agtcgcagtg ggtgttgaag aggccggcgg
      121 ccaccttggc ccacttgccc acgtggccga agagatggac cggtcccgct ccccgggacg
      181 cgcactcttt cagggcgtgt cccacgtggt cccccatgcg gacgatgagc tccctgggcg
      241 ccccgaaccg ctccgccagg catttttccc ctacgtagcc ggggacgagg aagaccgtgc
      301 cgggacgggg ggagtcgaag gcctgggccg aggagatgta gagatcgatg gaggccaggt
      361 aggcggcggt ggacttgggc tccacgatgc ccgtggtgcc caggatggag atgcccccct
      421 cgatgccgag gcgggggttc caggttgttt tcgtccgctc ttccccctcg ggggcggaga
      481 tagcgatctc cacgccctgt ccttcgagaa ggagggcccc cacctccgac tcgatcatgc
      541 gccgggggcc ggggttgatg gcccattcgc cgggggggac agggagtccc ttccgggtga
      601 ccttcccgat gccgggaccg ccggagaggg tcactcccgg gcgggaagac ggacgggctt
      661 cggcaacgat gagcatgccg gaggtgccgt cggggtcgtc gcccgagtcc ttccggacgg
      721 cgcaccgggc gagcttttcc gaaagggtgc actcctcaag gggaagggtg agggactgcc
      781 ctcctggaag ggtgaccgtg acagaggaca ggggggcgcc ggaaaggaac atccgggcgg
      841 ctcccttcgc cgccgccgct gcgcaggttc ccgtggtgta gcccctccgc aggcccccgg
      901 tgtatcccag tccgtcgaag ggcttcccgt ccacggtcct actccttttc cgcgaggcgg
      961 ataagggcgt tcacgattgc cgccgccacc gggctgcctc ccctggggcc gggggcggtg
     1021 atccaggggc agtccagagt caccgtctcg tcgtgggatt ccgcggcacc cacgaagccc
     1081 acgggaattc ccaccacgag ggcgggtttg cagaggccct ttttcaccag gtcgcacacc
     1141 tcgaagaggg ccgtgggggc gttcccaatg gccacgatgg agccgtcgag gaggggcagg
     1201 gccttccgca tggccgcccg ggccctggtg gtggcggccc gcttcccgtc ctccccc
//
LOCUS       DAOTHD010000791         1252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_131212_length_1252_cov_3.159566, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000791 DAOTHD010000000
VERSION     DAOTHD010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131212_length_1252_cov_3.159566"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="P5219_11435"
     CDS             <1..619
                     /locus_tag="P5219_11435"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HRX27422.1"
                     /translation="VGLALPLALFQGYGPGEILAAMAAGPRVATGNPAVDELLGRGGL
                     LFLAGVVAVVCAAYVFAGQLEATGTFRRVSSLLRKKFIGRSRGRFVLSASLTGMTVAV
                     ATGNSYLSEILPGTMFRRLADEMDISRRVLSRTLEDSGTVVVPLVPWSAAAVYMSSVL
                     GVSAWEYFPWAVICYSGVPAAWLLAFSGIALWPSDRREEKKRPGR"
     gene            complement(646..>1252)
                     /locus_tag="P5219_11440"
     CDS             complement(646..>1252)
                     /locus_tag="P5219_11440"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRX27423.1"
                     /translation="PGERLLETALARELGMSRTPVREALGQLASTGFLVKSESARGYQ
                     IPVLTPKDIEDVFFMRGILESKAAMLCARSASEETVRELRRINTREEETFKANLKGEY
                     AELNEDFHMLLADSSDNPYMKMYIRQLYWRSNLYVLFFMSFYCLESDYDTKRLSYMEH
                     RKVVDAVEGGDPGAAEAAMKDHILSSYNHLLFPKKPLEGMS"
BASE COUNT          185 a          391 c          408 g          268 t
ORIGIN      
        1 cgtgggcctt gcccttcccc tggccctgtt ccaggggtac ggtcccgggg agatcctggc
       61 cgccatggcc gccggacccc gggtggccac gggaaaccct gcggtggatg agctgcttgg
      121 caggggaggg ctgctcttcc tggcgggagt ggtcgccgtt gtctgcgcgg cctatgtttt
      181 cgccggccag ctcgaggcta cggggacgtt ccgcagggtc agttcccttc tccgaaagaa
      241 gttcatcggc cgcagcaggg gaaggttcgt cctctcggcc tctctcacgg ggatgaccgt
      301 ggccgtcgcc acggggaatt cctacctgag cgaaatcctt cccggcacca tgttccgccg
      361 tctcgccgac gagatggaca tctcccggag ggtcctctcc cgcacactgg aggactcggg
      421 gacagtggtg gtccccctcg ttccgtggtc cgccgcggcg gtgtacatgt cgtcggtgct
      481 tggcgtctcc gcgtgggaat actttccctg ggccgtgatc tgctattccg gcgtgcccgc
      541 agcctggctg ttggcctttt cggggatcgc cctctggccg tcggaccggc gggaagaaaa
      601 aaaacgcccg gggcggtgag ccccgggcgt ttcattcata ccggatcagg acattccctc
      661 caggggtttt ttcgggaaca gcaggtggtt gtagctgctg aggatgtggt ccttcatggc
      721 ggcctcggcg gctcccgggt cccccccttc cacggcgtcc acgaccttcc ggtgctccat
      781 gtagctcagg cgcttcgtgt cgtagtccga ctccaggcag taaaaactca tgaagaagag
      841 gacgtacagg ttcgacctcc agtagagctg ccggatgtac atcttcatgt aggggttgtc
      901 cgacgaatcc gctaggagca tgtggaagtc ctcgttcagc tccgcgtatt cccctttcag
      961 gttggccttg aaggtctcct cctcccgggt gttgattctc cggagctccc ggactgtttc
     1021 ttcggaagcg gagcgggcac agagcatggc ggccttgctc tccaggatgc cccgcatgaa
     1081 gaagacgtct tcgatgtcct tgggcgtgag aactggaatc tggtaccccc gcgcactttc
     1141 ggacttgacg agaaaacccg tggacgcaag ctggcccagg gcctcccgca cgggagtcct
     1201 gctcatgccg agttccctgg cgagggccgt ttcgagaagg cgttcgccgg gg
//
LOCUS       DAOTHD010000792         1249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_131720_length_1249_cov_2.353434, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000792 DAOTHD010000000
VERSION     DAOTHD010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131720_length_1249_cov_2.353434"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="P5219_11445"
     CDS             complement(<1..277)
                     /locus_tag="P5219_11445"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRX27424.1"
                     /translation="MTGPDEAEYIALTAKSLIADGADPAAIAVLYRTNFQSRSLEEAF
                     INMDVPYQLLGTKFFERKEVKDVLSYLRLALNPGSNADLTRIINEPAR"
     gene            complement(255..455)
                     /locus_tag="P5219_11450"
     CDS             complement(255..455)
                     /locus_tag="P5219_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27425.1"
                     /translation="MTPTAKARLKAFIAVVAWGGSLPSIKAAVAEVSFTVRRRGRRDL
                     AGADGSGPGPRWGDRNDRTGRG"
     gene            complement(452..>1249)
                     /locus_tag="P5219_11455"
     CDS             complement(452..>1249)
                     /locus_tag="P5219_11455"
                     /inference="COORDINATES: protein motif:HMM:NF012410.2"
                     /note="molecular chaperone; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HRX27426.1"
                     /translation="WKEFGPVIKEGLASFDGAEKENREAILEICLFPSASSGDGFLSL
                     GEYVEAMKEGQQAIYYITGRKLPVLRNSPLLERCREKGFDVLLLADPVDEVIAPTLPD
                     FRGKKFRSLEREDALPPEEEKTDAPRPEGLASFLREVLSETVKDVKISFRLTESPACL
                     VSDDDGASFNMERILRSMGREVPPLKRILEINPDHPVISKMNRLLASGGGEEKVKEYA
                     FLLHDQCVLAEGGQIADPALFAGRLSSLLSENMDAAGGIFPPAGEGE"
BASE COUNT          269 a          361 c          345 g          274 t
ORIGIN      
        1 cgcgagcagg ttcgttgata atgcgagtca gatcagcatt actgccgggg tttagtgcta
       61 gtcggagata cgacaaaaca tccttaactt cctttcgctc aaagaactta gtaccaagga
      121 gttggtatgg cacatccata ttgataaaag cttcttcaag tgagcgagat tggaaattag
      181 tgcggtagag aacagctatt gcagccggat ctgcgccatc agcaatcagg cttttggcgg
      241 ttaaagcgat atattcagcc tcgtccggtc cggtcattcc ggtcgcccca gcgcggcccc
      301 gggcccgagc cgtcggcacc cgccaggtca cgcctgccgc gacgccgcac ggtgaaggag
      361 acctcagcga ctgcggcctt gatggaaggc agcgagcctc cccatgccac caccgcgata
      421 aaggccttga gccgagcctt tgcggtgggt gtcattcccc ttcaccggca ggcggaaaaa
      481 tgcctccagc ggcatccatg ttttctgaaa gaagagagga cagcctaccg gcgaaaaggg
      541 ccggatcggc gatctgccct ccttccgcga gaacgcactg gtcgtgcagc aggaaggcgt
      601 attccttcac tttttcttcc ccccctcccg aagcgagcag cctgttcatt ttggagatca
      661 ccgggtgatc agggttgatt tccaaaatgc gtttcagtgg aggaacctcc ctgcccatgg
      721 accggaggat gcgctccatg ttgaacgatg ccccgtcgtc gtcgcttacg agacaggccg
      781 gtgattccgt gaggcggaag gatattttga catccttcac cgtttcggag aggacctccc
      841 tcaaaaacga ggcaagtcct tccggccggg gagcgtctgt tttttcctcc tcgggaggca
      901 gggcatcctc ccgttcgagg gaacggaatt tctttcccct gaaatccggt agggtggggg
      961 cgatcacctc gtccaccgga tcggcgagga gaagcacgtc aaagcccttt tccctgcacc
     1021 tttcaagaag gggggagttc ctgagcaccg ggagctttct tcccgtaatg taataaattg
     1081 cctgctgccc ttctttcatg gcctccacgt attctccgag ggaaaggaaa ccatcccccg
     1141 aagaagcaga cgggaaaagg caaatttcca ggatggcctc cctgttttct ttttcggcac
     1201 catcgaagga agcgagcccc tccttgatga ccggaccgaa ttctttcca
//
LOCUS       DAOTHD010000793         1249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_131759_length_1249_cov_1.943049, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000793 DAOTHD010000000
VERSION     DAOTHD010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131759_length_1249_cov_1.943049"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="P5219_11460"
     CDS             <1..1100
                     /locus_tag="P5219_11460"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27427.1"
                     /translation="PFQSLGLNFGIVIGAMSGQVAIAIARYFEMGGISGLLFCFAVAM
                     VAAAFCGYFTGKLYNKTRGQEMIASLIVGFFANGIYQFLFLFVVGVIIAVPATHPMIK
                     PDGIGIRMTVDLVPVKQGGLKYALDNIYQIPFVHALLIVAVGLLLLLAVRYWLNIRKG
                     RSYLNSPLSLVVNGGLCLAIAGVAIHAMVTKSPLMMVRKVPVVTGLLIIGLCVFTVLI
                     MKTKLGQDFRSVGQSQHTAEVSGINVDRTRIIATMISTVLASWGQIIYLQNMGTLNTY
                     NAHTQIGMFSVAALLVGGASTSKAGITHAIFGTILFNAMFIMSPEIGQSLFGQAILGE
                     YFRTFMVYGVIGVALGIHVWKANKKSRIMVE"
BASE COUNT          241 a          395 c          332 g          281 t
ORIGIN      
        1 ccccatttca aagtctcggg ctcaacttcg gcattgtcat cggcgccatg tccggccagg
       61 tggccatcgc catcgcccgg tactttgaaa tggggggcat atcgggcctt ctgttctgct
      121 tcgccgtggc catggtggct gcggccttct gcggctactt caccgggaag ctctacaaca
      181 agacccgtgg ccaggaaatg atcgccagcc tcatcgtcgg gttcttcgcc aacggcattt
      241 accagttcct cttcctcttt gtcgtcggcg tgattatcgc cgttcccgca acccatccca
      301 tgatcaagcc cgacggcatc ggcatacgga tgactgtgga ccttgttccg gtcaaacagg
      361 gtggtctgaa atacgccctg gacaacattt accagatccc cttcgtccat gctcttctta
      421 tcgttgcggt gggactgctc ctgctgctgg ccgtccggta ctggctcaac atacggaaag
      481 gccgctccta cctgaacagc cccttgtccc tcgtcgtcaa cggcggcctg tgtctcgcca
      541 tcgcaggtgt ggcgatccac gccatggtca cgaaatcacc gctcatgatg gtgcgcaagg
      601 ttcccgtggt gacggggctg ctgatcatcg gcctctgcgt gttcacggtt ctcatcatga
      661 agaccaagct ggggcaggat ttccggtcgg tggggcagag ccagcacact gcggaagtct
      721 cggggatcaa cgtggacagg acgaggatca tcgccaccat gatctccacc gtactggcgt
      781 cctgggggca gatcatctac ctccagaaca tgggcaccct gaacacttac aacgcccaca
      841 cccagatcgg catgttttcc gtggcggccc tcctcgtggg aggcgcctcc acctcgaagg
      901 ccggcataac ccacgccatc ttcgggacca tcctcttcaa cgccatgttc atcatgtcgc
      961 ccgagatcgg ccagtccctc ttcggacagg ccatactggg agaatacttc aggaccttca
     1021 tggtctacgg cgtcatcggc gtggcccttg gcatccacgt ctggaaggcc aacaagaaga
     1081 gccggatcat ggtggaatag gcaatctcac cgccggtaca aacagagccc cccgcttttc
     1141 cggcaaggaa aggcgggggg ctctgttaca ttctcgtact ctttcgcgcc acaataagtt
     1201 ttacctctca atcgtttttt caacagtcat gtgaatggaa atcgcagta
//
LOCUS       DAOTHD010000794         1246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_132183_length_1246_cov_2.510495, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000794 DAOTHD010000000
VERSION     DAOTHD010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132183_length_1246_cov_2.510495"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="P5219_11465"
     CDS             <1..529
                     /locus_tag="P5219_11465"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164423.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HRX27428.1"
                     /translation="SERPRTIGRVRSFFGNFGVLVRAYTYIRTLGPDGIREVSEMAVL
                     NANYLAKKLSSVYPLAHRGHCMHEFVLNGGPLKHETGVSSMDVAKALIDAGYHPPTVY
                     FPLIVPEALMVEPTETESRETLDRFAETMEAIAAKARAEGPEAFHDLPVTTPVSRPDE
                     TTAARNPVLRWQFEK"
     gene            622..>1246
                     /locus_tag="P5219_11470"
     CDS             622..>1246
                     /locus_tag="P5219_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRX27429.1"
                     /translation="MYDLVILGGGPGGLRAAELAAAAGLKTVLIEKDRLGGVCLNRGC
                     IPTKALYAHIVGGKGPREGLWSRVEAVMDKLRQGSATALRMAGAKMVRGTGTVTQWEG
                     EKKVSVRKEDGSEEEITGARLLLATGARSVRPSFGGNDLPEVLTGDWAITDPALWDPE
                     RNGEVRTVAVLGAGVIAMEMAMMLQDLGKQVLLLKHSDQILRRLDGDI"
BASE COUNT          248 a          411 c          414 g          173 t
ORIGIN      
        1 cagcgaacgg cccaggacca tcggaagggt ccggagcttt ttcggaaact tcggcgtcct
       61 cgtccgggcc tacacctaca tccgcaccct cggaccggat ggcattcggg aagtgtcgga
      121 gatggcggtg ctgaacgcca attacctggc gaaaaagctc agctcagtct accccctggc
      181 ccacagggga cactgcatgc acgaattcgt cctgaacggc ggccccctga agcacgaaac
      241 gggcgtcagc agcatggacg tggccaaggc cctcatcgac gccgggtacc acccgcccac
      301 ggtgtacttc cctctcatcg tcccggaggc cctcatggtg gaacccacgg agacggaatc
      361 cagggagacc ctggaccggt tcgccgagac catggaagcc atcgcggcga aggcgagggc
      421 ggaggggccg gaggccttcc acgatctccc ggtcacgacg cccgtctcca ggccggacga
      481 gaccactgcg gcccgcaatc cggtcctgcg ctggcagttc gaaaaatagc ccattacgga
      541 aacagggggc gcccgggcgg cgcccccttc gttcaggacc gcctcttcac gggctgaagt
      601 cctgcggaag gagagagaga catgtacgat ctggtaatcc tcggcggagg tcccggaggg
      661 ctccgggccg ccgaactcgc ggcggccgcc gggctgaaga ccgtcctcat agaaaaggac
      721 cgcctgggag gcgtctgcct caaccggggg tgcatcccca ccaaggccct ttacgcccat
      781 atcgtcggcg ggaagggccc gagagaaggg ctgtggtcca gggtggaagc cgtcatggac
      841 aagctccgcc agggttcggc cacggccctc cgcatggccg gggccaaaat ggtccgggga
      901 accggcacgg tgactcaatg ggaaggggaa aagaaagtct ccgtcaggaa ggaggacggg
      961 agcgaagagg aaatcaccgg cgcccggctg ctgctggcca ccggagcccg gtcggtccgg
     1021 ccctccttcg ggggcaacga cctgccggaa gtcctcaccg gcgactgggc catcaccgac
     1081 cccgccctct gggacccgga acggaacggt gaggtccgca ccgtggcggt cctcggagcg
     1141 ggggtcatcg ccatggaaat ggccatgatg ctccaggacc tcgggaaaca ggtactcctt
     1201 ctgaaacact cggaccagat cctccgccgc ctggacgggg acatca
//
LOCUS       DAOTHD010000795         1246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_132241_length_1246_cov_1.602855, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000795 DAOTHD010000000
VERSION     DAOTHD010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132241_length_1246_cov_1.602855"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="P5219_11475"
     CDS             complement(<1..894)
                     /locus_tag="P5219_11475"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRX27430.1"
                     /translation="MKKFLTAAAALILAAGCFGAVLSSGAAEAAWPEKNITVIITHGT
                     GGDTDFNARLMCRLLEQKLGVSVVPTNVTGSNGAIAMSQYKDGTPDGYTFLMTNTAAL
                     TGNEATGLSDFGYDAFEPVSVYGKQSGENIIVPADSPYKTLADLIEASKKNPNTIKFG
                     ISTGGGVYIASVILEQAAGTKFAVIDEGDAASRLTALLGKHVDATIAPYAVVREYIQA
                     GQVRTLGTLLKEPPALLKDIPTAHESGCPELILNTLYVCLAPKGTDPAAVKALNAAMM
                     DISANSEEYKKEVNRYNLQEPW"
     gene            complement(1035..>1246)
                     /locus_tag="P5219_11480"
     CDS             complement(1035..>1246)
                     /locus_tag="P5219_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27431.1"
                     /translation="GFIKAQIPLTPMILGFILGPMFELNLRKVSQLFMMNPLSFAERP
                     IAIGFFIVTAAVVMLNIRANRRALD"
BASE COUNT          212 a          324 c          403 g          307 t
ORIGIN      
        1 ccacggctcc tgcaggttgt acctgttgac ttctttcttg tactcttcgc tgttggctga
       61 gatgtccatc atggcggcat tgagggcctt gaccgccgcg gggtctgttc ccttgggagc
      121 gaggcagaca tagagcgtat tgaggatgag ttcggggcag cctgattcgt gggctgtggg
      181 gatgtccttc agcagtgcgg ggggttcctt caggagtgtc ccgagcgtcc tgacctggcc
      241 ggcctggata tattccctga ccacggcgta gggagcgatg gtggcgtcaa cgtgcttgcc
      301 cagcagggcc gtcagccggg atgcggcgtc tccttcgtcg atgacggcga actttgttcc
      361 cgcagcctgt tcaagaatga cggaagcgat gtacacgccg ccgccggtgg aaattccgaa
      421 cttgatggtg ttgggattct ttttggaggc ttcgatgagg tcggccagag ttttgtaggg
      481 agagtcggcc ggaacgatga tgttctcacc cgactgcttt ccgtagacgc tgaccggctc
      541 aaaggcatcg taaccgaaat cggagaggcc ggtggcctcg ttgcccgtga gggcggcggt
      601 gttggtcatg aggaaggtgt agccgtcagg ggtcccgtcc ttgtactggg acatggcgat
      661 ggccccgttg ctgccagtga cgtttgtggg aacgacggag acgccgagct tctgctcaag
      721 caggcggcac atcaggcgcg cgttgaagtc ggtgtcgccg cctgttccgt gggtgatgat
      781 gacggtgatg tttttctcgg gccaggccgc ttcagcggct cccgatgaaa gaacggcgcc
      841 gaaacaccct gctgccagga taagcgctgc tgctgcggtc aaaaactttt tcacgttttt
      901 tcctcctccc gaattctgct gcgtctgaga tgtttccctt ctgtttttcg tccgcttcgg
      961 gcttgagccc gtttccggtt tcttcggatt cactccattg catcactcct ggcgtgtcaa
     1021 tctgccgtcc ggcgtcagtc cagggctcgt ctgtttgccc tgatgttgag catgaccacc
     1081 gccgcggtca caataaaaaa tccgatggct atgggacgtt ctgcaaagga cagggggttc
     1141 atcatgaaga gctgggatac cttgcgaagg ttgagctcga acataggtcc caggatgaat
     1201 ccgaggatca taggggtgag ggggatctgt gccttgatga atccgt
//
LOCUS       DAOTHD010000796         1245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_132297_length_1245_cov_3.326891, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000796 DAOTHD010000000
VERSION     DAOTHD010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132297_length_1245_cov_3.326891"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="P5219_11485"
     CDS             complement(<1..265)
                     /locus_tag="P5219_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27432.1"
                     /translation="MKRKRILRVSLLFLVSALFLCAAAVSPSCADYSTSGTVGGPHPA
                     TYTIKCNPGDSFTVSVGSNYPTSVDILFMTPAGNEWAYNSVAGS"
     gene            complement(302..685)
                     /locus_tag="P5219_11490"
     CDS             complement(302..685)
                     /locus_tag="P5219_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27433.1"
                     /translation="MPPDVYARMSEQSKIKATAMVESVKTLETTKQSTWKTVVFLLRH
                     PMSEGVPEKFSGTCYSVDHEWQQPPAGGTIYFYPEKGDLVYVTVAADGGSITSYTYLN
                     DVMESLFIDNAEKIRYGMGNAWLDL"
     gene            943..>1245
                     /locus_tag="P5219_11495"
     CDS             943..>1245
                     /locus_tag="P5219_11495"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRX27434.1"
                     /translation="MNGRKGWILLCILSAGILFFTAAQAGASVPSGEELRLSPAPAFV
                     TGAGVNVRTGPSTASRSLGKLDGQASEELVATDGETGEDGKIWYLVLSGKVGEGWIR"
BASE COUNT          287 a          342 c          344 g          272 t
ORIGIN      
        1 cgctaccagc cacggaattg tatgcccatt cgtttcccgc gggggtcatg aagaggatat
       61 ccaccgatgt gggatagttc gaccccacgc tgaccgtgaa actgtcgccg gggttgcact
      121 tgatggtgta ggtggcggga tgggggccgc cgacggtacc cgatgtggaa taatccgcac
      181 aggatggaga aactgccgca gcacacagaa acagcgccga gaccaggaaa agcagggaaa
      241 ctctcaggat tctctttctt ttcatatcgc tctcctttcc ggggttgagc gcaaccgtct
      301 cttaaagatc cagccaggcg ttccccatgc cgtatcgtat cttctcggca ttgtcgatga
      361 acaggctctc cataacatcg ttcagatagg tgtagcttgt gatggaaccg ccgtccgcgg
      421 ccaccgtcac gtagacaagg tcgccctttt cggggtaaaa atagatggtt ccccccgccg
      481 ggggctgctg ccactcgtgg tccaccgaat agcacgtccc ggaaaacttt tccggcactc
      541 cttccgacat cggatgtctc aacaaaaaaa cgacagtttt ccaggtgctc tgtttcgtgg
      601 tctcaagcgt ctttaccgat tccaccatcg ccgtcgcctt gatcttcgac tgttccgaca
      661 ttcgggcgta cacatccggc ggcatcacgc cctccgccga aaaacagaag gctgctaaga
      721 gaaaaacaag cacagaagcg gcaactctgc agtttttcaa ttactccccc tcccctgtgt
      781 tcggattttt cttcacgggc gcactcccgg ataaactttt tctgcccgct tcgggagaat
      841 aaccacggag gaccggaaga taaaacgagg ctactttttg aaattaactc aatgttataa
      901 tatcatccgg tccgttgaag ctcaagggaa gggggctttc cggtgaacgg aagaaaaggg
      961 tggattctgc tctgcattct gtcggcagga attctgtttt tcaccgctgc tcaggccgga
     1021 gcgtcagttc catcaggaga ggaactccgg ctgagtcccg caccggcatt cgtcacggga
     1081 gcgggagtga acgtgagaac cggtccgtcc acagcctcca gaagcctggg aaaacttgac
     1141 ggacaggcat cggaggaact cgtggcgacc gacggcgaaa cgggagagga cggcaaaatc
     1201 tggtacctgg ttctttcggg aaaagtcggt gaagggtgga tcagg
//
LOCUS       DAOTHD010000797         1244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_132475_length_1244_cov_2.328848, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000797 DAOTHD010000000
VERSION     DAOTHD010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132475_length_1244_cov_2.328848"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1186)
                     /locus_tag="P5219_11500"
     CDS             complement(<1..1186)
                     /locus_tag="P5219_11500"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRX27435.1"
                     /translation="MDYQWHFKDELNTLAPKTSYVALFKPWNWIVGTGVYIDDAEKSI
                     AAWKMRFVAAGLLSIAAAGAVSLFLSLRAALLGKKAARAALLDRSLKEKTLELARSES
                     QLALITQIYRNAAEGIMITDADGRILEVNRAFSAITGYGPEEIIGKTPDMLKSDKHGP
                     DFYREMRDALRKDGHWSGEIWDRRKNGEAFPSRLNIFAYTGEERNVSRYIGVYQDLSE
                     LHRSRDLLQHEVNHDGLTGLPNRFLFHDRLALAISRSREKSRILSVIMIGLDRFGAVN
                     KTLGYPVGDGLLQEAGKRLSEAAAEPGAVARFGGDEFVVLLTWGGTYSSCLRVVERML
                     ASLRNPFSVGGQSYRITASAGVTFYPRDGESPEELLQNASLSMERAKREGGNSWRLFT
                     EDL"
BASE COUNT          209 a          383 c          397 g          255 t
ORIGIN      
        1 caaggtcttc ggtgaacagc ctccatgagt ttcccccttc ccgcttggcc cgctccatgg
       61 agagggaggc gttctggagg agttcctcgg gggattcgcc gtcccgggga taaaaggtga
      121 ctccggcgct ggcggtgatc cggtaggact gtcccccgac ggaaaacggg ttcctgagcg
      181 aggcgagcat ccgttccaca accctgagac aggaggaata cgtcccgccc caggtgagca
      241 ggacgacgaa ctcgtcccct ccgaaccggg caacggcccc cggttcagcg gccgcttcgg
      301 agagtctctt tcccgcctcc tggagcagtc cgtcccccac gggatagccc agggtcttgt
      361 tgactgcccc gaaccggtcc aggccgatca tgatcaccga aaggatacga cttttctccc
      421 gggagcggga aatggcgaga gcgagcctgt cgtggaagag aaaccggttc ggcaggcccg
      481 tcagcccgtc gtggttcacc tcgtgctgca gcagatcccg gctccggtgg agttcgctca
      541 ggtcctggta gacgccgata tagcggctca cgtttctctc ctcgccggtg taggcgaaga
      601 tgttcagccg ggagggaaag gcctcgccgt tcttccggcg gtcccatatc tctcccgacc
      661 agtggccgtc cttcctgaga gcgtcccgca tctcccggta aaaatcgggg ccgtgcttgt
      721 ccgatttcag catgtccggc gttttgccta tgatctcttc cgggccgtag ccggtgatgg
      781 ctgagaaagc ccggttgacc tcgaggatgc gcccgtccgc gtcggtgatc atgatgccct
      841 cggcggcgtt ccggtagatc tgggtgataa gggcgagctg gctctcggac ctggcaagct
      901 ccagggtttt ttccttcagg ctccggtcca ggagcgcggc ccgggctgcc ttttttccga
      961 ggagggccgc ccggaggctc aggaagagag agaccgcacc cgcagcggct atcgaaagca
     1021 gcccggcggc cacgaaccgc attttccatg cggcgatgct tttttccgcg tcgtcgatgt
     1081 agactcccgt tccgacgatc cagttccacg gtttgaaaag ggcaacgtag gatgttttgg
     1141 gggccagggt gttcagttcg tccttgaagt gccactggta gtccattatt cccccccctt
     1201 cgggggaaga gacggtggtt tcgatccggt caaagagctg gcgg
//
LOCUS       DAOTHD010000798         1242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_132837_length_1242_cov_2.016849, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000798 DAOTHD010000000
VERSION     DAOTHD010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132837_length_1242_cov_2.016849"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..386)
                     /locus_tag="P5219_11505"
     CDS             complement(162..386)
                     /locus_tag="P5219_11505"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRX27436.1"
                     /translation="MAVTIRLLGLFRQYGPPEPWEVAAGERSIGELLAEIEEKAHAGY
                     IPIVNGERKSKTYVPAEGDEIKIMPLVTGG"
     gene            complement(405..>1242)
                     /locus_tag="P5219_11510"
     CDS             complement(405..>1242)
                     /locus_tag="P5219_11510"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HRX27437.1"
                     /translation="RLGIDTISCGAWIGFLMECWEYGWITPQDTGGLSLKWGDPDVLL
                     ALVEQIGTMTGMGGWFREGIRGAARHIGREAEKITVEVKNMDYPAHDPRAVYALGVNY
                     AVGTRGACHERGNPQTSALGGYFPELGQDGPYDRQSPEYAAKTAVIHQDVSVLMNCMT
                     ICQFMMGGGGLTLTEIRPAYRYLTGKDVPVEELVKYGERAWQLQRITNVRDGLDRKDD
                     TLPLKMTVPAMVGPRAGKTPTPHDRILDDYYELRGWDRNGIPTRERLESLGLGRFAAF
                     LP"
BASE COUNT          200 a          418 c          367 g          257 t
ORIGIN      
        1 caccacgatg aaggtgatga aaaacgcccc gaactgctgg ttgatgggat tgaagcccgt
       61 ggtctgggag tttcccacga caaggatcga gttctccggg tattgcgcat catgcgacat
      121 gtctgaattc taccacggaa ctccggacca tgtctacttt tttatccccc cgtcaccagg
      181 ggcatgatct ttatctcgtc cccttcggcg gggacatagg tcttgctttt ccgttcaccg
      241 ttcacgatgg gaatatagcc cgcgtgggcc ttctcctcaa tttccgcgag aagctcgccg
      301 atggaccgct ctccagccgc aacctcccag ggttccggag gtccgtactg cctgaagagg
      361 cccaggagac ggatcgtcac agccatggta ccgggtcctc cgcctcaggg aaggaaagcc
      421 gcgaaccggc cgagtcccag ggattccagg cgttccctgg tggggatgcc gttcctgtcc
      481 cacccccgga gctcgtagta gtcgtcgaga atccggtcgt ggggggtggg cgtcttcccc
      541 gcccttggcc ccaccatggc ggggacggtc atcttcaggg gcagggtatc gtccttcctg
      601 tccaggccgt cccgcacgtt ggtgatcctc tggagctgcc acgcccgctc gccgtacttc
      661 accagctcct ccaccggaac gtccttcccc gtcaggtacc ggtaggccgg ccgaatctcc
      721 gtcagggtca ggccgccgcc gcccatcatg aactggcaga tggtcatgca gttcatcagg
      781 acgctcacgt cctggtggat caccgccgtc ttcgcagcat actcgggaga ctgcctgtca
      841 taggggccgt cctggccgag ctccgggaag tatccgccga gggcgctcgt ctggggattg
      901 ccccgttcgt ggcaggctcc cctggtgccc acggcgtagt tcaccccgag ggcgtacacc
      961 gcccgggggt cgtgggccgg gtaatccatg ttcttcactt ccacggtgat cttttccgct
     1021 tccctgccga tatgccgggc cgcgccccgg atcccttccc ggaaccatcc gcccatgcct
     1081 gtcatggtgc cgatctgttc caccagggca aggaggacgt ccgggtcgcc ccatttcagg
     1141 gacagcccgc cggtgtcttg gggcgtgatc cagccgtact cccagcactc catgagaaag
     1201 ccgatccagg ctccgcatga gatggtgtct atccccagac gg
//
LOCUS       DAOTHD010000799         1241 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_132930_length_1241_cov_2.974705, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000799 DAOTHD010000000
VERSION     DAOTHD010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132930_length_1241_cov_2.974705"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="P5219_11515"
     CDS             complement(<1..469)
                     /locus_tag="P5219_11515"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRX27438.1"
                     /translation="MTDHRIPPMDRPWPSKLFVEVTTACNLRCAMCVKQSGPGIPDAY
                     LSDETFEKLVPAFPTLETLILNGIGEPLLHPGLEHYIRTARQNVPADAVIGFQTNGSL
                     LTEQRACSLLSAGTDVISISVDAADPALFRSMREGGDTEDVNRAAAALGRAKER"
     gene            complement(466..>1241)
                     /locus_tag="P5219_11520"
     CDS             complement(466..>1241)
                     /locus_tag="P5219_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRX27439.1"
                     /translation="PDLPFPTSAGFVSLQDLADLKETMGPTQILRKDRQRSITVDANV
                     SGTTVGEAYQVIDSKLKEIPLPPGYRFTYAGEIQSIQDNFGALGFALGLAVLLTFLLI
                     AAILESFVFAFVIMLTVPLSIIGVVPALILTNTPMSIYGLMGVIMLVGLVVNNAIVII
                     DYAEIVRKEGMDPAEAIIEACRVRLRPILMADATSVIAMVPLAMGVGAGGSYRSPMAI
                     VSIGGLVAGGVLALLAIPPVYKLVWRLKQRFGFEAVEQP"
BASE COUNT          255 a          354 c          405 g          227 t
ORIGIN      
        1 tccgttcttt tgccctcccg agagcggcgg cggcccggtt tacatcttca gtatcccccc
       61 cttccctcat ggaacggaag agggccggat cggctgcatc cacggaaatg ctgatgacat
      121 ccgttccggc ggaaaggagg gagcacgccc gctgttccgt caggagcgag ccgttggtct
      181 ggaaacctat cacggcatca gcgggaacgt tctgcctcgc cgtgcggata tagtgttcca
      241 gaccgggatg aagcaggggt tccccgatgc cgttcaggat gagggtttcc agcgtcggga
      301 acgccgggac cagtttttcg aaagtttcat cggaaaggta cgcatccggg attccgggcc
      361 ccgactgctt gacgcacatg gcgcagcgga ggttgcacgc cgttgtcacc tcgacgaaga
      421 gcttggaagg ccagggacgg tccatgggag gaatccggtg atccgtcatg gctgctccac
      481 cgcctcgaag ccgaagcgct gcttcagtct ccagaccagc ttgtacactg ggggaatggc
      541 cagaagggca agaacgccgc ccgccacgag cccgccgatg gaaacgatgg ccatgggaga
      601 cctgtaggat cctccggccc cgacgcccat ggcaaggggg accatggcga tgacggacgt
      661 ggcgtccgcc atgagaatcg gccggagtct gaccctgcac gcctcgatga tggcttcggc
      721 gggatccatc ccctctttcc tgacgatttc ggcgtagtcg atgatgacga tggcgttgtt
      781 caccaccagc cccacgagca tgatcacgcc catgaggccg tagatggaca tgggggtgtt
      841 cgtcaggatg agggccggca ccactccgat gatggagagg gggacggtca gcatgatcac
      901 gaaggcgaaa acgaaggatt cgaggatggc cgcgatgagg aggaatgtca gaagtaccgc
      961 cagcccgagg gcaaagccca gcgccccgaa attatcctgg atggactgga tttctcccgc
     1021 ataggtaaac cggtatcccg ggggaagagg aatctccttc agtttgctgt cgataacctg
     1081 gtatgcttct cccacggtcg taccggagac gttggcgtcc acggtaatgg accgctgccg
     1141 gtccttccgg agaatctggg tgggtcccat ggtctctttc aggtccgcca gatcctgcag
     1201 ggaaacaaag ccggccgagg tgggaaaggg aaggtcggga a
//
LOCUS       DAOTHD010000800         1239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_133243_length_1239_cov_2.880912, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000800 DAOTHD010000000
VERSION     DAOTHD010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133243_length_1239_cov_2.880912"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="P5219_11525"
     CDS             <1..727
                     /locus_tag="P5219_11525"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRX27440.1"
                     /translation="VAQAAGQCVESSAKTTGFTKDVARKLEEVVSDIEAVSARSQENR
                     AKMSALAEAVDSIAGFVGAITGFADQTNLLALNAAIEAARAGEAGRGFAVVADEVRKL
                     AEESNRAASEISNLMSSLSGYAGESIKATEEEEKTLRTVVEKAGILQQTLKASMDELS
                     AMDAELNKVSELARDQSHSNIEIAGAVDNIAQGTSNIVERLEEIRNSAQAAREAFESV
                     TDQSRTLLQGMEEMERQLGQFKV"
     gene            1064..1237
                     /locus_tag="P5219_11530"
     CDS             1064..1237
                     /locus_tag="P5219_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysW"
                     /protein_id="HRX27441.1"
                     /translation="MSTKVQCIVCEGDVILPAEVLEGELLTCPDCGTELEIVSLNPVT
                     VAEAPEVQEDWGE"
BASE COUNT          300 a          325 c          369 g          245 t
ORIGIN      
        1 cgttgcacaa gcggcaggac agtgcgtgga gtcgtcggcg aagacgacag gatttacgaa
       61 agacgtcgcc cgcaagctgg aggaggttgt ctcggatatc gaggcggtca gcgcccgttc
      121 acaggaaaac cgggcaaaga tgtccgccct ggcggaggcg gtcgattcga ttgcgggttt
      181 cgtcggcgcc ataacgggat ttgctgacca gacgaacctg ctcgcgctga acgccgccat
      241 cgaggcggca agggccggag aggcgggacg gggctttgcc gtcgtggcag acgaagtccg
      301 gaagctcgcc gaagaatcca accgcgctgc gagcgaaatt tccaatctta tgagctcgct
      361 ctcgggatat gcgggagagt ccatcaaggc cacggaggaa gaagagaaaa cgctgcgcac
      421 cgtggtcgaa aaggcgggga tcctccagca gaccctgaag gcaagcatgg atgaactttc
      481 cgccatggac gcggagctga acaaagtctc ggaactggcc cgcgaccagt cccattcgaa
      541 tatcgaaatt gcgggggctg tggacaacat cgcccaggga acatccaata tcgttgaacg
      601 gctcgaggaa atcaggaatt ccgcccaggc cgcccgtgaa gcgttcgaat cggtgacgga
      661 tcaatcgcgg acgctcctcc aaggtatgga ggagatggaa cgccagctcg gtcagtttaa
      721 agtttaaccg cttcccgcct ccggatgacg ttcctcctgc gggtcctgta cacggggtga
      781 tttcggggag tgaatttccg ggaatgataa tgaccgcaac ggaggtaccg cacctgacgc
      841 gcggtacctc cggctttttt tgaatacttc catccgaagt cacgggcggc attgtgttcc
      901 cctcatattc caggagcaca ccaaaatcaa ccagcgacag taattcctgc cgttttccgt
      961 aagaaacata cagaaaaaag aaacatttat ttcttacttg acgaaaaagg aacacaggtt
     1021 tacaatcccc ccattgtaaa tatttatttt ggagggattc tttatgtcaa ctaaagttca
     1081 gtgtattgtc tgcgaagggg acgtcattct tcccgccgaa gttctggagg gcgagcttct
     1141 tacatgcccg gactgcggca cggaactcga gatcgtttct ctgaacccgg tcaccgtggc
     1201 cgaggccccc gaagtgcagg aggactgggg agagtagat
//
LOCUS       DAOTHD010000801         1238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_133385_length_1238_cov_3.641589, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000801 DAOTHD010000000
VERSION     DAOTHD010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133385_length_1238_cov_3.641589"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1238
                     /locus_tag="P5219_11535"
     CDS             <1..>1238
                     /locus_tag="P5219_11535"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HRX27442.1"
                     /translation="ERSYGALLLNSYHSGFPWTDGITEGIRSTFESGGLRINLDVEYM
                     DTKRTSSVRAVRAFGEYFRTKYADKRPDILLSSDDDALVFLLRHGKELFPGVPIVFCG
                     LNNPGFIDSGGDARVTGVFEEMDIPGTVELMLRLFPQTRNLALVSDLSISGMGSVSLV
                     RKALAPFLDRLKVVDLLGFSGDELRDELSMLSPDTAVLLLVYFQDDAGEYYSPARSVA
                     LVNSAGPFPVFGLWSMMVEGGALGGSVLIAERHGALAAEMALRILRGTPPSEIPPVAD
                     RHLVPVFNYREMVRFGLSRFDLPAGSVLLSEPETFLYRHWKLLLANALVLSAALVYLL
                     ALFINERMKKAAEKDLKLKKAQWEGLFRNAPEAYVVFDGANDIVTINDGFTRLFGYTR
                     KEVAGKNLDSVVADFPGIRS"
BASE COUNT          225 a          384 c          356 g          273 t
ORIGIN      
        1 cgagaggtcc tatggtgccc ttcttctgaa ttcctaccac agcggctttc cctggaccga
       61 cggcataacg gagggcatcc gctcaacctt cgaatccggc ggcctgagga tcaatcttga
      121 cgtggagtac atggacacca aacgtacgtc atcggtcagg gcggtaaggg ccttcgggga
      181 gtatttccgg accaagtacg cggacaagcg acctgacatc ctcctcagtt ccgacgacga
      241 cgccctcgtc ttcctgctcc gccacggaaa ggaacttttt cccggcgtcc ccatcgtctt
      301 ctgcggcctg aacaacccgg ggtttatcga ttccgggggc gatgcccggg ttaccggcgt
      361 cttcgaggag atggacattc ccggaacggt ggagctgatg ctccgcctct tcccccaaac
      421 gaggaacctc gccctcgtga gcgatctttc catttcgggc atggggtccg tcagcctggt
      481 ccggaaagcc ctggcacctt ttcttgacag gctgaaggtc gtcgaccttc tcggtttctc
      541 aggcgatgaa ctccgggatg aactttccat gctttctccc gacacggcgg tcctccttct
      601 cgtgtatttc caggatgacg ccggagagta ctattccccg gccaggagtg tcgcccttgt
      661 gaacagcgcc ggtcccttcc cggtcttcgg tctctggtcc atgatggtgg agggtggagc
      721 cctgggcgga agcgtcctta tcgctgaacg gcacggggcc ctggctgcgg agatggccct
      781 tcgaattctg cggggaacgc ctccttcgga gatcccgccg gtcgccgacc ggcacctggt
      841 tcccgtattc aactacaggg agatggtccg gttcggcctt tcacggtttg accttcccgc
      901 cggatcggtt ctcctgtccg aaccggagac gtttctctac aggcactgga agcttctcct
      961 cgccaacgcc cttgttctct cggccgccct ggtgtatctc cttgccctgt tcatcaacga
     1021 gcggatgaaa aaggcggcgg aaaaggacct gaaactgaaa aaagcccagt gggagggact
     1081 cttccggaac gctccggagg cctacgtcgt cttcgacggg gccaacgaca tcgtaacgat
     1141 aaatgatggt ttcaccaggc tcttcgggta tacccggaaa gaagtggcag gcaaaaatct
     1201 cgactccgtg gtggccgatt tccccgggat cagatccg
//
LOCUS       DAOTHD010000802         1238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_133434_length_1238_cov_2.658495, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000802 DAOTHD010000000
VERSION     DAOTHD010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133434_length_1238_cov_2.658495"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..1032)
                     /locus_tag="P5219_11540"
     CDS             complement(184..1032)
                     /locus_tag="P5219_11540"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRX27443.1"
                     /translation="MKCRSVFWLLVVLSLLAALPAAGSEEAVLTVGDDKVSGDEILYL
                     LSLELGGNDALAALRLKEMTEEERADFLERVSTALLFSRGAVLKGLHIDPKIAAQLRW
                     NQVNLLANAYIASLEPRLTFGDNELKAAYEKNRQKYVRKGSARIRHIFVTSGEKGRSA
                     LLALLSGEDFYSVAGRLSEDSASSASGSETGWVEEGTLPESLDRLVFSAPLNTVLGPV
                     EAGAGFYIFEVMERSEGRSLSFAEAKKLVASDLQKTVLSGEAASLRKRFPVILRPAAL
                     GNTPLH"
     gene            complement(1048..>1238)
                     /locus_tag="P5219_11545"
     CDS             complement(1048..>1238)
                     /locus_tag="P5219_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27444.1"
                     /translation="HDYPITVDEAADLGLPVVPNDQDSFCNLMSLYPQSTSARPSVQY
                     IPVPYPGRPSAHSRRDIY"
BASE COUNT          288 a          320 c          346 g          284 t
ORIGIN      
        1 gggttttctt cccctttcaa gctccatgta tatccatcct ttctgtttcc gcgatttttc
       61 cgccttcgtt tcagggcgtt ttctcattat aaagagagaa atttcatctg tccataaatt
      121 acactgttca tggagaaaga aaaaagggtg cagcataagc cgcaccccgg aaacagcctg
      181 gaatcagtgc aagggggtat tcccgagagc agctggtctc aagatcacgg ggaaccgttt
      241 ccggagagaa gcggcctctc ccgagaggac tgtcttttga aggtcagagg ctaccagttt
      301 ttttgcttca gcaaaggaga gagaacggcc ctcacttcgt tccatcacct cgaaaatgta
      361 gaatccggcg ccagcttcca cgggcccgag gacagtattg agcggggcgg agaatacgag
      421 cctgtcaaga ctttcaggga gggtcccttc ttctacccac cccgtttcgc ttcccgacgc
      481 ggatgaggct gaatcctcac tgaggcgccc ggcgacggaa tagaaatctt ccccggaaag
      541 aagggcaaga agggccgatc ttcctttttc tccggaggtg acaaaaatat gcctgatcct
      601 ggcgcttcct ttgcggacat atttttgccg gttcttttca tacgcagctt tcagttcatt
      661 gtcaccgaag gtcagccggg gttcgagcga ggcgatatag gcatttgcca ggaggttgac
      721 ctggttccag cgcaactggg cggctatttt cgggtcaatg tggagtccct tgagaacagc
      781 ccccctggaa aagagaagag ctgtggatac tctctcgagg aagtccgctc gttcctcttc
      841 ggtcatttct tttaatcgga gcgccgcaag ggcatcgttt ccaccaagtt ccaggctgag
      901 cagatataaa atttcatccc cggaaacctt gtcatctccc actgtaagga cggcctcttc
      961 agaacctgcc gcgggaaggg ccgccaggag agagagaaca acaagaagcc agaagacgga
     1021 tcggcatttc atggcagaac ctccatttca gtagatgtcc ctgcgggaat gggcggaggg
     1081 gcggccgggg tagggcaccg gtatatattg aaccgaaggc cgtgcggagg tggattgagg
     1141 atacaggctc atcaggttgc agaaggaatc ctgatcgttc ggcaccacag gcagaccgag
     1201 atcagccgct tcgtccacgg tgatcgggta atcatggg
//
LOCUS       DAOTHD010000803         1235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_133941_length_1235_cov_2.418644, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000803 DAOTHD010000000
VERSION     DAOTHD010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133941_length_1235_cov_2.418644"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="P5219_11550"
     CDS             <1..842
                     /locus_tag="P5219_11550"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HRX27445.1"
                     /translation="IVPYVRGRFVSRPPEDILEEMRTLVADGVLEITLLGQNVNSYGS
                     DFSDGYTFANLLRDGALKTGVRLLRFATSHPRDFTPDIVDTMAEHSPVICPSINLPIQ
                     SGSDRILALMNRGYTVERYRETVSVIRSRLPGAGLTTDLIVGHPGETEEDFRESLKVL
                     EEFRFDLVHSAAYSPREGTAAARREDQVPREEKMARLNELNSLQSGIALEINESLVGS
                     LFTVLLDDAAPRGEGLLQGRTPSDKVVLVEAGEEMLGRFVRVEVTRADNWCLSGRIVD
                     NGK"
     gene            856..>1235
                     /locus_tag="P5219_11555"
     CDS             856..>1235
                     /locus_tag="P5219_11555"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HRX27446.1"
                     /translation="MKKGFFSDPKFQGLAFLALGLGCFLLAGLLVFFFSGRFVDRPSG
                     ATAGPALSITAPVDKPSGPPAGEVSPPAPEPPETKEWVLYITGGVASPGVYSLPPGSR
                     VHNLVDAAGGLLPTADPVKVNLAAP"
BASE COUNT          223 a          402 c          384 g          226 t
ORIGIN      
        1 gcatcgtacc ctacgtccgg ggccgcttcg tctcccgtcc tcccgaggac atccttgaag
       61 agatgagaac cctggtggcc gacggtgtcc ttgaaatcac cctgctgggc cagaacgtga
      121 acagctacgg aagcgacttc tccgacggct acaccttcgc gaacctcctg agggacggcg
      181 ccctgaagac gggagtccgg ctcctccggt tcgccacctc ccacccccgg gattttaccc
      241 ccgatatcgt ggatacaatg gcggagcatt ctccggtgat ctgcccctcc ataaaccttc
      301 ccatccagtc gggaagcgac cgcatcctcg ccctcatgaa caggggatat acggtggaac
      361 gttacaggga gaccgtttcc gtcatccgga gccgtctgcc gggagccggc ctcaccacgg
      421 acctcatcgt ggggcacccc ggcgaaacgg aagaggattt ccgggaatcg ctgaaggtgc
      481 tggaggaatt ccggtttgac ctcgtccatt ccgccgccta ctctccccgt gagggaaccg
      541 cggcggcacg ccgggaagac caggtgcccc gggaggagaa aatggcccgg ctgaatgaac
      601 tgaacagcct ccagtcggga atcgcgctgg agatcaatga atctctcgtg ggcagcctct
      661 tcaccgtgct gctggacgac gcggcccccc ggggggaagg actcctgcag gggcggaccc
      721 catcggacaa ggtggttctc gtggaagcgg gggaggagat gctcggccgc tttgtccggg
      781 tggaggtcac ccgggcggac aactggtgcc tctcgggaag aatcgtcgat aacgggaaat
      841 agggagggac gggcagtgaa gaagggcttt ttctccgatc cgaaattcca gggacttgcc
      901 ttccttgccc tggggctcgg gtgttttctc cttgcaggtc tcctggtgtt ctttttctcc
      961 ggacgttttg tggaccgtcc ctcgggagcg acggcaggac ccgccctctc cataaccgca
     1021 cctgtggaca aaccctccgg tcctccggcg ggagaagtat caccccccgc ccccgagcct
     1081 ccggagacga aagagtgggt gctctacatc accggcggcg tggcctcgcc gggggtctac
     1141 tccctccctc cgggaagcag ggttcacaac ctcgtggacg ccgcgggggg actgcttccc
     1201 accgctgacc ccgtgaaggt aaacctggcc gctcc
//
LOCUS       DAOTHD010000804         1234 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_134088_length_1234_cov_2.793045, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000804 DAOTHD010000000
VERSION     DAOTHD010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134088_length_1234_cov_2.793045"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="P5219_11560"
     CDS             <1..603
                     /locus_tag="P5219_11560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX27447.1"
                     /translation="ELAVLLGAAGSGKSTLLNILGGLDVPTGGRLFYREDELTGADED
                     LLTAFRRDSVGFVFQFYNLIPSLTARENVALVTEIAPDPMTPEEALELVGLEDRMDHF
                     PSQLSGGQQQRVAIARAIAKRPEVLLCDEPTGALDVTTGITVLEAVEKVNRELGTLAV
                     VITHNAAIASMADRVIFLSGGKITSIRGNERRMPARSLSW"
     gene            600..>1234
                     /locus_tag="P5219_11565"
     CDS             600..>1234
                     /locus_tag="P5219_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27448.1"
                     /translation="MSAMRALDRKLLRDLSGMKGQMTAIALVMACGLSMMIMARGLVV
                     SLEETERRYYGTSRFADVFCELKRAPLSLESSLAALPGVAAAETRARGVLTLDIPGMR
                     EPVDGMIFSLPEDRPQRLNLLHLKAGRLPVPGSRDEIAVSSGFAGAHGFVPGDELVGT
                     VYGVRRRFRITGIAMSPEFIYETRAGEMLPDPKRFGVFWMNSGELSRALGL"
BASE COUNT          200 a          409 c          405 g          220 t
ORIGIN      
        1 gagctggcgg tgctcctcgg cgctgcggga agcggcaagt ccaccctgct caacatcctc
       61 ggcggcctcg atgtccccac gggggggcgc cttttctaca gggaggacga gctcaccggc
      121 gccgacgagg atctgctcac cgccttccgc agagattccg tgggctttgt cttccagttc
      181 tacaatctca tccccagcct gaccgcacgg gaaaacgtgg ccctggtgac ggaaatcgcc
      241 ccggatccca tgacccccga ggaggccctc gaacttgtcg gactggagga caggatggac
      301 catttcccct cccagctctc cggggggcag cagcagcggg tggccatcgc ccgggccatt
      361 gcgaagcgtc cggaagtcct cctgtgcgac gaacccaccg gggcccttga cgtcacgacc
      421 ggcatcaccg tcctcgaggc ggtggaaaag gtgaacaggg aactggggac ccttgcggtg
      481 gtgatcaccc acaacgccgc catcgcttcc atggccgatc gggtgatctt cctctccggc
      541 gggaagatca cgtccatccg ggggaacgag cgccgcatgc cggcacgttc cctctcctgg
      601 tgagcgccat gcgggctctc gacaggaagc tcctccggga tctttccggg atgaaggggc
      661 agatgacggc catagccctc gtcatggcct gcggtctctc aatgatgatc atggccaggg
      721 ggctcgtggt ctccctggag gagacggagc gaagatacta cggcacgtcc cggttcgccg
      781 acgttttctg cgagctgaag cgggcgcctc tctccctcga atcctccctg gccgccctgc
      841 ccggagttgc cgcggcggaa acgcgggccc ggggcgtttt gaccctggac attccgggaa
      901 tgagggagcc cgtggacggc atgatttttt cccttccgga ggaccgtccc cagcggctga
      961 accttcttca cctgaaggcg ggacggctgc ccgtcccggg cagcagggac gaaatagccg
     1021 tgagcagcgg cttcgcgggc gcccacggct ttgtgcccgg cgacgagctt gtcgggacgg
     1081 tctacggtgt ccgccgccgg ttccggatca ccggcatcgc catgtctccg gagttcatct
     1141 atgaaacccg ggcgggagag atgctccctg acccgaagcg gttcggcgtt ttctggatga
     1201 acagcgggga gctgtcccgg gccctcggcc tgga
//
LOCUS       DAOTHD010000805         1232 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_134505_length_1232_cov_1.847069, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000805 DAOTHD010000000
VERSION     DAOTHD010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134505_length_1232_cov_1.847069"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="P5219_11570"
     CDS             complement(<1..566)
                     /locus_tag="P5219_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27449.1"
                     /translation="MGRFSELDPGKIKTYAASDRVSKVRTAFQAVPPRKGMSVRDFLK
                     GLPDILKAKDLLNVARDIAEAKKKGKPVIAMLGGHVIKCGCAPVLLGLAKKGFISSFA
                     SNGAGAIHDAELAAWGQTSEDVAAQLDDGSFGMASDTAEILNSAAKRACELQKGLGES
                     LGELLEERQCSFREHSLAAGSLRLQIQYT"
     gene            750..>1232
                     /locus_tag="P5219_11575"
     CDS             750..>1232
                     /locus_tag="P5219_11575"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRX27450.1"
                     /translation="MRSILLTGGAGFIGSNLVRHLVRGKGVSVVNYDKLTYAGNLESL
                     RDLEGDPRHVFVHGDIVDREHLGAVLAKYSPDAVMHLAAESHVDRSIDGPGEFISTNV
                     VGTFTLLEAVRGYWNSLDKGAAESFRFLHVSTDEVYGSLGSDGFFTETTPYAPNSPYS
                     A"
BASE COUNT          268 a          349 c          317 g          298 t
ORIGIN      
        1 gtatattgaa tttgaagcct caggcttccc gcggcaaggg agtgttcccg aaaggagcat
       61 tggcgttctt caagcagttc accgagggat tcacccaacc ctttctgaag ttcacatgct
      121 ctctttgccg cactgttcag gatttccgcc gtatccgatg ccattccgaa agaaccgtcg
      181 tcaagctgag ccgcgacatc ttccgatgtc tgtccccagg cagcaagctc ggcatcgtgg
      241 atcgcccccg ctccgtttga agcaaagctg gaaatgaatc ctttttttgc caggccaagg
      301 agcactgggg cacagccgca cttgatgacg tggccgccaa gcattgcgat gaccggtttg
      361 cctttctttt ttgcttccgc tatgtcccgt gctacattga gaagatcttt tgccttcagt
      421 atgtcgggga ggcctttcag aaagtccctg acggacatcc cctttcgtgg cggaacagcc
      481 tgaaatgcag tcctgacttt tgaaaccctg tcggaagctg cataggtttt aatctttcca
      541 ggatccagtt ctgaaaatcg gcccatcagg ggtcacctca gaaaaatgat cttatttcaa
      601 acaaaaaatg tctctcggaa aaccgctaca cactgcaatg taacggttca ttatatcagg
      661 agagggtgat aaaatgaaaa tgatgaaaca ttttattgaa tggtattgag tacttttttg
      721 aaagtaattc catgcaggag gatttaatag tgcgtagtat tctcctcacc ggcggagccg
      781 gtttcatcgg cagcaacctc gtgcggcacc ttgtgcgcgg gaaaggtgtt tcggtcgtaa
      841 attacgacaa gctcacctac gcggggaacc tggagtcgct gcgggatctt gaaggagatc
      901 cccggcacgt ttttgttcac ggcgacatcg tggaccggga gcatcttggc gccgtgctgg
      961 cgaaatacag ccccgacgcc gtgatgcacc tggctgcaga gtcccacgtg gaccggtcca
     1021 tcgacggccc cggcgagttc atctccacca acgtggtggg caccttcacc ctccttgagg
     1081 ccgtccgggg gtactggaac tccctggaca aaggggcggc ggagtccttc cgtttcctcc
     1141 acgtctccac ggacgaggtc tacggctccc tcggttcaga cggcttcttc accgagacca
     1201 cgccctacgc ccccaactcc ccctattccg ca
//
LOCUS       DAOTHD010000806         1229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_134924_length_1229_cov_3.625213, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000806 DAOTHD010000000
VERSION     DAOTHD010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134924_length_1229_cov_3.625213"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="P5219_11580"
     CDS             complement(<1..1189)
                     /locus_tag="P5219_11580"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRX27451.1"
                     /translation="MKTVIVNTLEAPAGYTAPAGMLDLKAAYDTSLPATKPSVEFDEN
                     NILILMYTSGTTGLPKGVVSRYNRNVVDRLRLLAGLILKPESVYYTALPLFHGNALFV
                     TTTQALIAGCTVALSKRFSASRFWEEISKSGASFFNTIGAIIPILMKTPESPYERNHK
                     VTHVFSAGCPADLWEPFEKRFGVIIWEAYAAIDGTGFIANFGDGPKGSIGKSVLSVIR
                     VVDDKGNDVPPMTTGELLFQVPQDKKSTVEYYKNEKATSEKTRGDWEYTGDLVYQDPD
                     GFIYFVGRSTDSMRRRGENVSAFDVEQAILKHPSVVEAAVYAVPSEMTEDEIMASVKL
                     VDGQKLTPQEMWEFLQDKLARFAVPRYIRIVDDFPRTETFRIKKNELKNHGVTADTFD
                     AESS"
BASE COUNT          199 a          369 c          395 g          266 t
ORIGIN      
        1 tgctgctctc ggcgtcgaag gtgtcggccg tcacgccgtg gttcttcagc tcgtttttct
       61 tgatgcggaa ggtctctgtc cggggaaagt catccacgat ccggatatag cgcgggacgg
      121 cgaatctggc gagcttgtcc tggagaaatt cccacatctc ctgcggggtg agcttctggc
      181 cgtcgacgag cttcaccgac gccatgatct cgtcctcggt catctcggac ggaacggcat
      241 acacggccgc ctcaaccacg gaggggtgct tgagtatggc ctgctccacg tcgaaggcgg
      301 acacgttctc tccccgtcgc cgcatggaat cagtgctcct gcccacgaag tagatgaacc
      361 cgtccgggtc ctgatagacc aggtcgcccg tgtactccca gtcacccctg gtcttctcgc
      421 tcgtggcctt ttcgttcttg tagtactcca cggtgctctt cttgtcctgg gggacctgga
      481 agaggagttc gccggtggtc atgggaggga cgtcgttgcc cttgtcgtcc acgaccctga
      541 taacactcag tactgacttt ccgatggatc ccttgggtcc gtcgccgaag ttggcgatga
      601 atcccgtgcc gtcgatggcc gcataggcct cccagatgat gactccgaac cgcttctcga
      661 agggttccca gagatcggcc gggcagcctg ccgagaagac gtgcgtcacc ttgtggttcc
      721 tctcgtaggg gctttccggc gtcttcatga gaatggggat gatggccccg atggtgttga
      781 agaagctggc gcccgacttc gagatctcct cccagaagcg gctcgcggaa aagcgcttgg
      841 acagcgccac ggtgcagcct gcgatgaggg cctgggtggt ggtgacgaac agggcgttgc
      901 cgtggaacag gggaagtgcg gtgtagtaga ccgactccgg cttgaggatc aggcccgcca
      961 ggagccgcag cctgtccacc acgttccggt tgtaccgcga caccaccccc ttgggcaggc
     1021 ccgtggtgcc ggacgtgtac atgaggatga ggatgttgtt ctcgtcgaac tccacggacg
     1081 gcttcgtggc gggcaggctt gtatcgtagg ctgccttcag gtcgagcatg cccgcaggcg
     1141 ccgtgtagcc tgcaggcgct tccagggtgt tgacgatgac ggtcttcacc tggggggtct
     1201 cctccgcgac gctccggtac aggtccacg
//
LOCUS       DAOTHD010000807         1222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_136062_length_1222_cov_2.823479, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000807 DAOTHD010000000
VERSION     DAOTHD010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136062_length_1222_cov_2.823479"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="P5219_11585"
     CDS             complement(<1..944)
                     /locus_tag="P5219_11585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRX27452.1"
                     /translation="MAKAPREQHPLNEGTPLNGTRVYGPGRGISDGDEASCPEILRDI
                     TRNKGKDRWIESLSSTLPGMLFQVRREPSGKEAINCIGGTLAARFGFRAGHATVDREE
                     FFRNVPSGDLPRVVRSFREAVENKDTWRCEFCLELPQGCRFCVEGTASPAEEPDGTPL
                     WFGYLADVTERKELEERLKDQLELQEQIFATIPVPLVLKDHHSRFLQVNEAFADFVNL
                     PRKEIMGKGPYEVFGPDLAELVFREDEEIRRTGETKMDLERRVKDGHGRLMWLKSYKG
                     PIRNSRGDVVGIVGGNMDISAVKDAEEALRESEARWKFA"
BASE COUNT          222 a          396 c          334 g          270 t
ORIGIN      
        1 gcgaacttcc accgggcctc gctctccctt aaggcttcct ccgcgtcctt gacggcggat
       61 atgtccatgt tgccccccac gatgcccacg acatctcccc ggctgttccg tatgggtccc
      121 ttgtaggatt tgagccacat gaggcggccg tggccgtcct tcaccctgcg ctcgagatcc
      181 attttcgtct cccccgtccg ccggatttcc tcgtcttccc ggaagaccag ttcggccaga
      241 tccggaccga agacctcgta gggccccttc cccatgatct ctttcctcgg gaggttcaca
      301 aagtccgcaa aggcctcgtt gacctggagg aaccgggagt gatgatcctt gaggacaagg
      361 ggcaccggga tggtggcgaa gatctgctcc tggagctcca gctggtcctt cagccgctcc
      421 tcgagctctt tccgctccgt tacatcggcg aggtagccga accacagggg agtgccgtca
      481 ggctcctccg caggcgacgc cgtcccttcg acacagaacc ggcagccctg gggaagttca
      541 agacagaatt cgcaccgcca ggtatcctta ttttcaacag cttccctgaa agaccggacg
      601 acccggggaa ggtcgccgga cgggacgttc cggaaaaact cctccctgtc caccgttgca
      661 tggcctgccc gaaatccgaa gcgggcagcc agggttccgc cgatgcagtt gatcgcctct
      721 ttgccggaag gctcccgcct cacctggaag agcatccccg gaagggtgga tgacaggctt
      781 tcgatccacc ggtctttccc tttattccgg gtgatgtccc ggaggatctc cgggcaggat
      841 gcttcatccc cgtccgaaat accccttccg ggaccataga ccctggtccc gttcagggga
      901 gtgccttcat tcaggggatg ctgttcccgg ggcgctttcg ccatggcaca ttcgcctcct
      961 gcaaaggtat ggcagcattc tcgatttctc acaatctgtt atcatagtac aaaatgtcgt
     1021 ttcatggaat tctttttctt atgaaacaga aacttttctc cctttcctgg acctgagcgt
     1081 accccctgtt ccccgggccg ggaagggctg cggaaaaaaa cgtcaacgaa cggagagatg
     1141 gccgtgaaaa cagtttcctc caccctgtgc tgcgatattc tcatcctcgg cggcggttcc
     1201 gccggggctg cggcggcatg gg
//
LOCUS       DAOTHD010000808         1221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_136227_length_1221_cov_3.129503, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000808 DAOTHD010000000
VERSION     DAOTHD010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136227_length_1221_cov_3.129503"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="P5219_11590"
     CDS             complement(<1..390)
                     /locus_tag="P5219_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27453.1"
                     /translation="MRKASGIFLVLFLFLTGFSSVVEAVTFESSGGGAAPVKKNGKPA
                     PSSRGESSKGTVARLDLPKNQKTRLADSLELVRLTGLQASLALRNLEELDTREATGEE
                     MKARLNHAVEACDALEKAGKRLEDSVRI"
     gene            733..>1221
                     /locus_tag="P5219_11595"
     CDS             733..>1221
                     /locus_tag="P5219_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27454.1"
                     /translation="MLPKKMFRCLLLVLLLALPSWGEELTLPPIEELPAGETPPAAPV
                     IPSLPAVPAFDAAPAFETSGSTGQPPAPPAGGDVLDETEFAKINWTGDYVEATGQAVP
                     PPGMENSPQGKLLARRGAVVDLQRNLLEAVQGVKVDSKTIMTDFMASDLVRTEVQGLI
                     KGV"
BASE COUNT          272 a          367 c          301 g          281 t
ORIGIN      
        1 tatcctcact gaatcctcca ggcgttttcc cgccttttca agggcgtcgc aggcctcgac
       61 ggcatggttc agccgggcct tcatctcctc cccggtcgct tctcttgtgt caagctcttc
      121 gagattcctc agcgcaaggc tcgcctgaag gcctgtgagc cgaacaagct cgaggctgtc
      181 cgccagccgc gttttctggt tcttcggcag gtcgagcctg gctaccgttc ccttgctgct
      241 ctccccgcgg gaggacgggg ctggtttccc atttttcttc accggggcgg ctccaccccc
      301 ggaactctca aaagtgactg cctccaccac tgaggaaaaa cccgtgagga aaagaaacaa
      361 aacaagaaaa attccgctcg ctttccgcat tgccgctcca ccgcctttca cataattctc
      421 aactcgtccg caggctcccc gaagcttcct gcgataatga gcccggacag ccgatacagc
      481 accacattcc gtcgcaccgg caaaaactct ctttacatta aaagacgttc aaaaatacaa
      541 taaaaaagga gaatatacag gcaaaaacaa ccaacaccca agggactcaa gactcattca
      601 agttgaaaac acaggcttat tgttttataa ttatacatca aaatgatcca catgattgtt
      661 attttttcct tcttcgcttt gtatgctttt acgttgtgat tttttttcgg acagacgaga
      721 gggggattca agatgcttcc aaaaaaaatg ttccgatgcc tgctgctggt tcttctgttg
      781 gcccttccct catgggggga ggaactgact cttcctccga ttgaggaact gccggcggga
      841 gaaactcccc ccgccgcccc ggtcattccc tcccttcctg cagttcccgc ctttgacgcg
      901 gccccggctt tcgagacaag cggttctaca gggcagcctc ctgctcctcc ggcaggggga
      961 gacgtgctgg atgaaacgga gttcgcaaag atcaactgga caggggacta cgtggaggca
     1021 accggtcagg ctgtgcctcc cccgggaatg gaaaattctc cccagggcaa gcttcttgcc
     1081 cgtcggggcg ccgtggtgga tctccagcgg aaccttctcg aggccgtcca gggagtgaag
     1141 gtcgactcga aaaccatcat gaccgatttc atggcctccg accttgtcag gaccgaggta
     1201 cagggcctga tcaaaggagt g
//
LOCUS       DAOTHD010000809         1215 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_137249_length_1215_cov_3.274138, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000809 DAOTHD010000000
VERSION     DAOTHD010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137249_length_1215_cov_3.274138"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="P5219_11600"
     CDS             <1..895
                     /locus_tag="P5219_11600"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HRX27455.1"
                     /translation="LSLFLPYAAGLSLNQVNPVLSRMLASFLQEGAISVLNYANRVIQ
                     LPLGLFVIAVSQAVLPQLSRCSSDSVDEFREIMRDSLRFALFVVLPVTLGMILVSREI
                     VHLLFFRGAFGTWAWEGTARSLAMYSVGLPGMACSTVVMRGLYARSLPRQALWVTLTS
                     VAATALFSLILMKPMSFGGLALATSLSFSLSGWVGVRLLSRNLGTGLGIFSLSWAGKM
                     AASLAVTAGVVFVYTIGMPYPAEGSLSLRAAWLGGIFLASCAAYGASTGLLRFQEWGW
                     LRGALSRKKSDREAKRSDGEE"
     gene            899..>1215
                     /locus_tag="P5219_11605"
     CDS             899..>1215
                     /locus_tag="P5219_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27456.1"
                     /translation="MTLKKIGALLLLLVFAAAGAAGAQDTDRLLDQRTANLWFEGEAL
                     GDLIIGARAQFAFIYVDGKLVDAAWSDPMAPAWLKNNTSFYGSKETRKKAMFIIRVRT
                     VRNL"
BASE COUNT          198 a          374 c          373 g          270 t
ORIGIN      
        1 gctctccctg ttccttccct acgccgccgg gctctccctc aaccaggtca accccgttct
       61 cagcaggatg ctcgcctcct ttctccagga gggggcaatc tcggtgctga actatgcgaa
      121 ccgggtcatc cagcttcccc tggggctctt cgtcatcgcc gtctcccagg cggtgcttcc
      181 ccagctttcc cggtgctctt cggacagcgt ggacgaattc cgggagatca tgagggattc
      241 tctgcggttt gccctgttcg tggttctccc ggtcaccctc gggatgatcc tggtctcaag
      301 ggaaatcgtc caccttcttt ttttccgggg tgctttcggg acgtgggcat gggaaggaac
      361 ggcccgttcg ctggccatgt attccgtcgg ccttcccggt atggcgtgct ccaccgtggt
      421 catgaggggg ctctacgccc gcagccttcc caggcaggcc ctgtgggtca ccctgacgag
      481 cgtggccgcc acggccctgt tctccctgat cctcatgaaa cccatgtcct tcggggggct
      541 tgccctggcc acttctctct ccttcagtct ttcagggtgg gttggggtga ggctgctctc
      601 cagaaacctc gggaccggcc tcgggatctt ttccctctcc tgggccggaa aaatggcagc
      661 ctccctggca gtgaccgcgg gggtagtctt cgtgtacact attggaatgc cctatccggc
      721 ggagggaagc ctttccctcc gggcggcgtg gctcggaggc atcttcctgg cgtcctgcgc
      781 cgcctatggg gcgagcaccg gattactgcg cttccaggaa tggggctggc ttcggggcgc
      841 cctgtcccgg aaaaagagtg acagggaagc caaacgatct gacggggagg aatgaaccat
      901 gacgttgaaa aaaataggag cgctcctgct tctgctggtt ttcgccgccg ccggtgcagc
      961 aggtgcgcag gatacggacc gcttgctgga tcagcggacc gcaaacctgt ggtttgaagg
     1021 ggaggccctc ggtgacctca tcatcggggc cagggcgcag ttcgccttta tttacgtgga
     1081 cgggaagctc gtcgatgcgg catggtcaga tcccatggcc cccgcgtggc tgaaaaacaa
     1141 cacgagtttt tacggcagca aggaaaccag gaagaaagcc atgttcatca tccgggttcg
     1201 aacggtgaga aacct
//
LOCUS       DAOTHD010000810         1211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_137965_length_1211_cov_3.830450, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000810 DAOTHD010000000
VERSION     DAOTHD010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137965_length_1211_cov_3.830450"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="P5219_11610"
     CDS             <1..980
                     /locus_tag="P5219_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HRX27457.1"
                     /translation="GDCRKILDRTEREFLSFGLPVKRTRKGALLATMKGRNDEEVRLV
                     SAHVDTLGAVVREIKPNGRLRLLQIGGFAWTSFETENLVVRTASGRDVRGSLLPEKAS
                     IHAFSDTVRETLRTDENMEVRLDEPVSSPEDVRALGIEVGDFVFFDPRYEVSESGFVK
                     SRFLDDKACVAFLFGAIKAVKDSGIPPAHTTVFYLTNYEEHGHGISCLPENTAEHLAL
                     DVGIVAPPANSREDAVTIVARDSRTPYDFAFRKFLSDLAAENNVPYRVDTHFRYGSDA
                     SLAAVAGFDANFACFGPGVDASHSYERTTIRAVEASGELLAAYLASERR"
     gene            997..>1211
                     /locus_tag="P5219_11615"
     CDS             997..>1211
                     /locus_tag="P5219_11615"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164794.1"
                     /note="catalyzes the release of the N-terminal amino acid
                     from a tripeptide; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HRX27458.1"
                     /translation="MSSVLDRFLKYVQIPSQAAHGKDTVPSTPGQMTLAVELGEELRA
                     LGLSDVTVDEHAYVTATLPANTDKKIPV"
BASE COUNT          225 a          401 c          362 g          223 t
ORIGIN      
        1 ccggggactg ccggaaaatt ctggacagga ccgaaaggga gtttctctcc ttcggccttc
       61 cggtcaagag aacccgcaag ggggcccttc tcgcgaccat gaaggggcgg aacgacgagg
      121 aagtccgtct cgtctccgcc cacgttgata ccctcggggc ggtggtccgg gagatcaagc
      181 ccaacggacg gcttcgcctc ctccagatcg ggggcttcgc ctggacctcc ttcgagaccg
      241 agaatcttgt cgtccggacc gcctcgggcc gggacgtccg gggaagcctt cttccggaga
      301 aggcctccat ccacgccttt tccgatacgg tccgggagac cctgcggacc gacgagaaca
      361 tggaggtccg cctggacgag cccgtttcct ccccggagga cgtccgggcc ctcgggatcg
      421 aggtgggaga ttttgtcttc ttcgaccccc ggtacgaagt ttcggaatcg ggtttcgtga
      481 agtcacgatt tctcgacgac aaggcctgcg tcgccttcct gttcggggcg ataaaggccg
      541 tgaaggattc cggaattcct ccggcccata cgaccgtgtt ctacctgacc aactacgagg
      601 agcacggtca cggtatctcc tgtttgccgg agaatacggc ggagcacctc gcccttgacg
      661 tggggatcgt ggcccctccg gcaaattcca gggaggacgc cgtgaccatc gtggcccggg
      721 acagccggac cccctacgac ttcgccttca ggaaattcct gtccgacctc gccgctgaga
      781 acaacgtccc gtacagggtg gacacccact tccggtacgg atcggacgcc agcctcgcgg
      841 ccgtggcagg tttcgacgca aatttcgcct gcttcgggcc cggcgtcgac gcgtcccatt
      901 cctatgaacg caccaccatc cgggcggtgg aggccagcgg agagcttctc gcggcctacc
      961 tcgcctcaga acgccgataa aggagattat tgccagatgt cgtcagttct tgaccgcttt
     1021 ttgaagtacg tacagatacc ctcccaggcc gcccacggca aagataccgt ccccagcacg
     1081 ccggggcaga tgacccttgc cgttgagctt ggagaggaac tgcgggccct gggcctttcc
     1141 gacgtgactg tggacgagca cgcctacgtc acggccaccc tccccgccaa cacggacaaa
     1201 aagatccccg t
//
LOCUS       DAOTHD010000811         1207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_138706_length_1207_cov_2.690104, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000811 DAOTHD010000000
VERSION     DAOTHD010000811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138706_length_1207_cov_2.690104"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..>1207
                     /locus_tag="P5219_11620"
     CDS             140..>1207
                     /locus_tag="P5219_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRX27459.1"
                     /translation="MTDTVRVRFAPSPTGALHIGGGHTALFNWLWARHMGGRFILRIE
                     DTDRERSTKEYEETIMSGMRWLGLDWDEGPDAGGDYGPYRQSERLPLYRKYADQLVDE
                     GKAYREGDAVIFKNPEGVKLIFDDVVYGHIEVMSETLKDTVLIKSDGMPTYNYAVVID
                     DHCMGITHVIRGEDHISNTPKQLLIYDALGWDYPAFGHLPMILGKDKKKLSKRHGATS
                     VYEFRDMGYMPDSVFNFLALLGWSAGENNEIFSRDEAVSLFELSRVTKRAAVFDMEKL
                     NFINQEHLKRLDPYTRLDLVEPFWREMGLPVDDHSREYLAQSLVVLCGRGQTAKEAAG
                     YSDYFLTFEAVKGRYDGSDVDD"
BASE COUNT          275 a          343 c          347 g          242 t
ORIGIN      
        1 gaagcagttt tttccccttc atggggatca catcctttat ctttttggtc cttttcggcc
       61 tgactgggtt ataataaccc attgtcggga ttcatgcaca aaagagttct gcactatcca
      121 acgtcaggga ggaacgaaaa tgacggatac tgtaagggtg cgattcgctc cgagccctac
      181 gggggcgctc catatcggcg gaggccacac cgccctgttc aactggctgt gggcccgtca
      241 catgggcgga aggttcattc tcaggattga ggacaccgac cgggagcggt ccaccaagga
      301 atacgaggaa accatcatgt cgggcatgcg ctggctcggc ctcgactggg acgaaggccc
      361 cgacgcgggg ggcgattacg gcccctacag acagtcggag cggcttcccc tgtaccgcaa
      421 gtatgccgac cagctcgttg atgaagggaa agcatacagg gaaggcgacg ccgtcatctt
      481 caaaaacccc gaaggcgtga agctgatctt cgacgacgtg gtctacggcc atatcgaggt
      541 catgagcgaa accctgaagg atacggtgct catcaaaagc gacggcatgc cgacctacaa
      601 ctatgcggtg gtcatcgacg accactgtat ggggatcacc cacgtcatcc ggggcgagga
      661 ccatatctcc aacaccccga agcagcttct catctatgat gccctcggat gggattaccc
      721 tgccttcggc caccttccca tgattctcgg gaaggacaag aaaaaactct ccaagcgcca
      781 tggggcaacc agcgtctacg agttccggga catgggctac atgccggact cggtcttcaa
      841 ctttctcgcc cttctcggct ggtccgccgg ggagaacaac gagattttca gcagggacga
      901 agccgtctca cttttcgagc tttccagggt aacgaagcgg gccgccgtgt tcgacatgga
      961 aaaactgaac ttcatcaacc aggagcacct gaagcgcctc gatccctata ccaggctcga
     1021 ccttgtggaa cccttctgga gagagatggg gcttcccgtg gacgaccatt cacgggaata
     1081 tctcgcccag tccctcgtgg tcctgtgcgg aaggggacag acggcgaagg aggctgcggg
     1141 atactccgat tatttcctca ccttcgaagc ggtgaagggc aggtacgacg gttcggacgt
     1201 ggatgat
//
LOCUS       DAOTHD010000812         1207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_138707_length_1207_cov_2.673611, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000812 DAOTHD010000000
VERSION     DAOTHD010000812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138707_length_1207_cov_2.673611"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..>1207
                     /locus_tag="P5219_11625"
     CDS             120..>1207
                     /locus_tag="P5219_11625"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRX27460.1"
                     /translation="MKKTSPRLFPILVFPPSTVRKMVLLLFFLPAVLLYLPQAGASEA
                     GRLSCDVVVAGGGAGGISAAITAARLGAKVILLEETEWLGGQMTAAGVSTMDDLSGNR
                     TGLYGEFHENIRFHYFMKGKSVSTCYWDGNTMAFEPSVGRDILVRMAKEASSGGKKGK
                     LDIFYRSRVTAVRREGPSVRGVTADLDGRKTAIDCSVLIDATEWGDVIPLAGALYRAG
                     NSLSPMISPKSRIQDITWLAVIKKYPAGIPQELRMQTPPPGYERFLPGFRKAVAKNGN
                     SFRSYPLRMPVDFATHNGYRGLPDSSNPENYDASTPEGWSAITKTGINWANDYPGGEK
                     WEARGGLPAAYLEDPEFRRKADAAALLKT"
BASE COUNT          254 a          374 c          361 g          218 t
ORIGIN      
        1 tcctcctttt ccagccttcc atccctattg tacaggccgg gggccgggat tgaacaggaa
       61 tagggattat gcaataatct gttctgacta taacaaatct gcaagaagga gcgttcgcca
      121 tgaaaaagac ttccccgcgc ctgttcccga tccttgtttt tcctccttca acggtgagaa
      181 aaatggttct tctcctcttt ttccttccgg cggtgcttct ttacctgccc caggcaggcg
      241 cctcggaggc gggacggctc tcctgcgacg tggtggtcgc aggcgggggc gccgggggaa
      301 tcagcgccgc cataacagcg gcacgactcg gtgcgaaggt catccttctc gaggagacgg
      361 aatggctcgg cggccagatg accgcagcgg gggtctccac catggacgat ctcagcggga
      421 accggacggg gctgtacggc gaattccacg aaaacatccg gttccactac ttcatgaagg
      481 gaaagtccgt ttccacatgc tactgggacg gaaacaccat ggccttcgaa ccctccgtgg
      541 gccgggacat cctcgtccgg atggcgaagg aggcttcttc cggaggaaag aagggaaagc
      601 tggacatatt ctaccggtcg agggtcactg ccgtccgcag ggagggaccg tcagtccgcg
      661 gggtcacggc ggatctggac gggcggaaga ccgccataga ctgttccgtc ctcattgacg
      721 ccaccgagtg gggcgatgtt attcccctcg cgggggcact ctaccgggcc ggaaactccc
      781 tgagccccat gatctcgccg aagagccgca tccaggacat cacctggctc gccgtaataa
      841 aaaaataccc ggcgggcata ccgcaggagc tcaggatgca gacccctccc cccggatatg
      901 agcggtttct ccccggattc cggaaggcag tggcgaagaa cggcaactcc ttcaggagct
      961 atcccctccg catgccggtg gatttcgcaa cgcacaacgg ctacagggga ctgcccgact
     1021 cgtccaaccc ggagaactac gacgcctcga cccccgaagg atggtctgcc attaccaaga
     1081 cgggcatcaa ctgggccaac gattaccccg gaggcgagaa gtgggaggcg cggggcggcc
     1141 ttcccgccgc ctacctggaa gatcctgaat tccgcaggaa agcggacgcc gcggccctgc
     1201 tgaaaac
//
LOCUS       DAOTHD010000813         1206 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_138875_length_1206_cov_2.927020, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000813 DAOTHD010000000
VERSION     DAOTHD010000813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138875_length_1206_cov_2.927020"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="P5219_11630"
     CDS             complement(<1..1079)
                     /locus_tag="P5219_11630"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRX27461.1"
                     /translation="MQGDDFAGRFGELFGCDEGTDRVRRALQIISTVFSSAVEGIVVT
                     DPRGIIRMVNPAFSSITGYSADEAAGRNPRILKSDRHPPEFYESFWRTLINEGKWEGE
                     IWNRRKSGEVYPEWLCCSAVHDEKGNVTDYIAVFNDLSEVHFKDAQIELRSNVDQLTG
                     LSSRTVFYSRLEGELERRDRDGRRLALLVLDINRFKNINDSLGHFAGDGVLQQVGARL
                     SRHLGGRDRASRIGGDDFHILIPDAPSVKEIASGAEKILSLLNDPVDAAGNRLFLSAS
                     LGIAVFPDDGRTAETLLKKADMAMHKAKELGISNYRFFTGELGEQASSKLLLENSLRA
                     GLGRGEFRLVYQPKYDLREDRVSGM"
BASE COUNT          222 a          392 c          361 g          231 t
ORIGIN      
        1 tccatgccgg acacccggtc ctcccggagg tcgtatttcg gctggtacac gaggcggaac
       61 tcgccccgtc caagcccggc ccgcagggaa ttctccagca gcagcttcga ggacgcctgt
      121 tctcccagct ctccggtgaa aaaccggtaa ttgctgattc ccagctcctt ggccttgtgc
      181 atggccatgt cggccttctt gagcagcgtt tccgccgtcc ggccgtcatc ggggaaaacg
      241 gcgatgccga gggaagcgct gagaaagagc ctgtttcccg ccgcgtccac gggatcgttc
      301 agcagggaga gaattttctc cgccccggag gcgatctcct tcaccgaagg ggcatcggga
      361 atgaggatgt gaaagtcgtc gccgccgatc cgggacgccc tgtccctgcc gccgagatgg
      421 cgggacagcc tggcgcccac ctgctgcagc acgccgtctc cggcgaaatg gccgagggag
      481 tcgttgatgt tcttgaagcg gttgatgtcc agcaccagga gggcgaggcg ccgcccgtcc
      541 cggtcccggc gctcaagctc cccttccagc cgggaataga acactgtccg gctcgaaagg
      601 ccggtaagct ggtcgacatt ggaccggagc tcgatctggg catccttgaa atggacctcc
      661 gacaggtcgt tgaagacggc gatgtagtcg gtgacgttcc ccttttcatc atggacggcg
      721 gaacagcaga gccactcggg gtagacttct ccgctctttc ttcggttcca gatctcccct
      781 tcccactttc cctcgttgat cagcgttctc cagaagctct cgtagaactc cggcggatgg
      841 cggtccgact tgagtatccg gggatttcgt cccgccgcct cgtcggcgct gtacccagta
      901 atggaggaaa aagccgggtt caccatgcgt atgatgcccc tgggatcggt aaccacgatt
      961 ccttccaccg cgctggaaaa cacggtggaa atgatctgca gggcgcgccg cactctgtcc
     1021 gtcccttcgt cgcagccgaa gagctcgccg aaccttccgg cgaaatcatc accctgcata
     1081 ggcaccatct cctccgtccc cgtcaaggat cagtgacgcc accgcgtcgg ccgcgccggg
     1141 gaaggcgatg gacgaggctg ccctttccat cgacgaaaga acggaccggt cggagaaaag
     1201 gccatc
//
LOCUS       DAOTHD010000814         1203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_139416_length_1203_cov_2.933798, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000814 DAOTHD010000000
VERSION     DAOTHD010000814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139416_length_1203_cov_2.933798"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="P5219_11635"
     CDS             <1..781
                     /locus_tag="P5219_11635"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HRX27462.1"
                     /translation="SVRSSAYYWGMIRLASLAGALPWAVGQSVGPFRTLSGMFFARNA
                     LGKCALRSVRDERSGALLNSWGQKSSRSPDPVFLLGRHSSCRERRDGHFLVNIRPWGG
                     SLPLRTVTKAARLAAEKKLPVIGVGLSREDVVLMERLAGSRIFRPRRIILLSAANWST
                     ESEQLFCNAAGAVSMRYHFALLALLSGVPLTLSAYDPKVEEFAAEWKLPVWKGDGRLP
                     HPALSPEQEKTVYRADLFLEEFSRMWNEARRLAHRKGTGRS"
     gene            778..>1203
                     /locus_tag="P5219_11640"
     CDS             778..>1203
                     /locus_tag="P5219_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRX27463.1"
                     /translation="MTPDLNGLAEHASAVRRDVVRMIGVARSGHLASSLSIVDLLVWL
                     YWEVLSLRTDEPSWEDRDRFVLGKGHGCPALYAVLANRSFFPREELWSYRRLGAMLQG
                     FPEVRRTPGVDAPGGSLGQGLGLANGLALALRDRASVPSV"
BASE COUNT          184 a          387 c          400 g          232 t
ORIGIN      
        1 gagcgtccgg tcgtccgcct attactgggg aatgatccgt ctcgcgtccc ttgccggcgc
       61 cctgccctgg gcggtaggac agtcggtggg cccgttccgg accctctctg gcatgttctt
      121 cgcccggaat gccctgggaa aatgcgccct cagaagcgtc cgggacgaac ggtccggcgc
      181 gctgctgaac tcctgggggc agaagtcctc caggtctccg gacccggtgt ttctgctggg
      241 gcggcattcc tcctgcagag aacggaggga cggccatttc ctcgtgaaca tccgcccctg
      301 gggcggaagc ctgccgctcc ggacggtgac gaaagccgcg agactggccg cggaaaaaaa
      361 actcccggtc atcggggtgg ggctttcccg ggaggatgtg gtcctcatgg agcggcttgc
      421 cggaagccgg attttccgtc cccggaggat tatcctgctc tcggcggcga actggagcac
      481 cgaatcggaa cagttgttct gcaacgccgc cggagcggtg tccatgcggt accatttcgc
      541 ccttctcgcc cttctctccg gtgttcccct gaccctctcg gcctatgacc cgaaggtgga
      601 ggagttcgcc gccgaatgga agctccccgt gtggaagggg gatgggcgcc ttccacaccc
      661 ggccctgtcg ccggaacagg aaaagaccgt ctatcgggcg gatctcttcc tcgaggaatt
      721 cagccggatg tggaatgaag cccggaggct cgcacacaga aaggggactg gtcgttcatg
      781 accccggatt tgaatggtct ggctgaacac gcttccgcag tccggaggga cgtggtcagg
      841 atgatcggcg tcgcccggtc gggacacctg gcgtcttctc tctccattgt ggatcttctt
      901 gtctggctgt actgggaggt tctttccctg cggacggacg aaccgtcctg ggaggacagg
      961 gaccggttcg tcctcgggaa ggggcacgga tgtcccgccc tgtacgccgt cctcgccaac
     1021 aggagcttct ttccccggga agagctgtgg agctaccgcc ggctcggcgc catgctgcag
     1081 ggatttcccg aggtccgcag gacccccggc gtggacgccc ccggaggatc cctcggccag
     1141 gggctcggcc tcgccaacgg cctcgctctc gcgctgaggg accgggcatc cgttccgtcg
     1201 gtc
//
LOCUS       DAOTHD010000815         1202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_139567_length_1202_cov_3.513514, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000815 DAOTHD010000000
VERSION     DAOTHD010000815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139567_length_1202_cov_3.513514"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="P5219_11645"
     CDS             complement(<1..186)
                     /locus_tag="P5219_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27464.1"
                     /translation="MRPNLSGVSRVMGRIEEIERKIRPQKDKKAREEKKDRFVDVLET
                     AERKEQTRPEAEEKSRRP"
     gene            complement(183..638)
                     /locus_tag="P5219_11650"
     CDS             complement(183..638)
                     /locus_tag="P5219_11650"
                     /inference="COORDINATES: protein motif:HMM:NF014144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HRX27465.1"
                     /translation="MNAKVNRFERILKTKVKVRDDERILLSMEKKEEERLLSVLATLG
                     TEKEQALASFGSQKDETFTVQDIWFRRKAIDLLDSRICREGESLCGVRQSIENTEARL
                     LEKHRDVKVMQKYISFLVEDLQDESKKQEQSELDDIAGIRHGSPKEGRR"
     gene            complement(702..>1202)
                     /locus_tag="P5219_11655"
     CDS             complement(702..>1202)
                     /locus_tag="P5219_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscN/YscN/HrcN family type III secretion system
                     ATPase"
                     /protein_id="HRX27466.1"
                     /translation="AIGEPPTTRGYTPSVFEMLPRLLERAGAGERGSITGIYTVLVEG
                     DDMNEPVADTVRGVLDGHVVLSRKIAARNFYPAVDVLESVSRVMPSIVSPEHLSASGR
                     LRELLAVYGEAEDLISIGAYKSGTNPKVDWAVEHLQGVHSFLRQTVGEKVPFEDMEER
                     LVSLVP"
BASE COUNT          189 a          380 c          328 g          305 t
ORIGIN      
        1 agggcgccgt gatttttctt ccgcctccgg ccgcgtttgc tcttttctct ctgccgtttc
       61 caggacatcc acaaagcggt ccttcttttc ttcccgggct tttttgtcct tttggggccg
      121 gatcttccgc tcgatctcct cgatgcggcc catgaccctc gacactcccg aaagattcgg
      181 tctcatcgcc ttccctcctt cggtgaacca tggcggatac cggcgatatc atccagctcc
      241 gactgttcct gttttttcga ttcgtcctgc agatcttcta ccaggaagga gatgtatttt
      301 tgcattacct tgacgtccct gtgtttttcc agcaggcggg cctcggtgtt ctctatggac
      361 tgcctcaccc cgcagaggga ttccccctcc cggcagatac ggctgtccag gaggtcgatg
      421 gcttttcgtc tgaaccagat atcctgcacg gtgaaggttt cgtccttctg ggaaccgaag
      481 gaggcgaggg cttgttcctt ttccgttccg agggtggcaa gaactgagag aagacgctcc
      541 tcctcctttt tttccatgga aaggagaatc cgctcgtcat cccgcacctt gaccttcgtt
      601 ttgagaatac gttcaaacct gttcactttc gcgttcattc cgtcctccgg tcgctgcctc
      661 tatttattca tcggcgggag actgccctgt cttgaccccg gtcagggaac cagggacacg
      721 agccgctctt ccatgtcctc gaagggcacc ttctctccca cggtctggcg gaggaaggaa
      781 tgaaccccct ggaggtgctc caccgcccag tccactttcg ggttggtgcc gctcttgtag
      841 gctccgatgc tgatgaggtc ttcggcttca ccatagacgg cgagcagttc ccggagcctt
      901 cccgaggcgg agaggtgttc cgggctgacg atggagggca tgaccctgct cacgctttcc
      961 agaacgtcca cggcgggata gaaattccgg gcggcgattt tcctcgacag gaccacgtga
     1021 ccgtcgagaa cgcctctcac ggtgtccgcc acgggctcgt tcatgtcgtc cccttccacc
     1081 aggacggtgt agattcccgt gatgctgccc cgctccccgg ctcctgcgcg ctcgagaagc
     1141 cgggggagca tctcgaagac cgacggggtg taccccctgg tggtgggagg ttccccgatg
     1201 gc
//
LOCUS       DAOTHD010000816         1199 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_140115_length_1199_cov_3.866259, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000816 DAOTHD010000000
VERSION     DAOTHD010000816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140115_length_1199_cov_3.866259"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="P5219_11660"
     CDS             complement(<1..1055)
                     /locus_tag="P5219_11660"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRX27467.1"
                     /translation="MKPEHRLLVVMTVSIFLGYMPWYGFSAVSSPMSEEFGLAASDMG
                     IILAVFQLGYVLTVIASGILADRIGNKPVLVGATLATGVFSLLFALFSRGFPSILAFR
                     LLTGLSAGAIYAPGMSLLSSWFPPDRRGMALGAYTAALTASYAGGYGIAAPLASLFSW
                     RWGITAISLPAFAAAVLLWRCVEDRPAFPGEAAPAAVRKEPFHLRAMFPGGPKAPLLL
                     TVAYCGHMWELYAFWGWIGPYLGACALSRGWPLQGASAFSGIAAAVIILFGSVAVVSV
                     GKLSDRAGRRRAILIAGTASVIGEFFFGFLEGIPLLLVLLPAAWIGFWSVADSAVYKA
                     GLADLTAPGMRGTALGI"
BASE COUNT          287 a          343 c          389 g          180 t
ORIGIN      
        1 tggatgccca gggccgttcc ccgcattccg ggggccgtga gatcggcaag ccccgccttg
       61 tacaccgccg aatccgccac cgaccagaag ccgatccacg ctgccggcag cagaacgagg
      121 agaaggggaa tgccttccag aaagccgaaa aagaattctc cgatgacgct cgccgttccg
      181 gcgatcagga tcgcccggcg acgtccggcc cggtcggaga gtttccctac cgacaccacc
      241 gcgacgctgc cgaaaaggat gatgacggca gcggcaatcc ccgagaaagc cgatgctcct
      301 tgaaggggcc acccgcggga gagggcgcat gcgccgagat acggtccgat ccatccccag
      361 aacgcataca gttcccacat gtggccgcaa tatgccacgg tgagcagaag aggtgctttc
      421 ggcccgccgg gaaacatggc gcggaggtga aagggttctt tgcgaactgc tgccggggcc
      481 gcttcaccgg ggaaagccgg tctgtcctca acgcagcgcc aaagaaggac ggcagcggcg
      541 aaagccggaa gggagatagc cgtgatgccc catcgccatg agaacagcga ggcaaggggg
      601 gcggcgatgc cgtatccgcc ggcataggag gccgtaaggg ctgccgtgta ggccccgagg
      661 gccatgcccc gcctgtcggg aggaaaccac gaggaaagga gcgacatgcc gggagcatag
      721 attgcacctg cggagaggcc ggtcagcagg cgaaaggcga gaatgctcgg aaagccccgt
      781 gaaaagagcg cgaaaagaag ggaaaagacg cctgtcgcaa gagtggcgcc cacgagcacg
      841 ggtttgtttc ctatcctgtc ggcaagaatg ccgctcgcga tgaccgtgag gacatacccc
      901 agctggaaaa cggcgaggat gatgcccatg tccgaggcag ccaggccgaa ctcctccgac
      961 atgggcgacg atacggccga aaaaccgtac cagggcatat atcccaaaaa gattgatact
     1021 gtcatgacga caagcagacg atgttcgggt ttcattccga tgacctcatt ttgactgtca
     1081 aaacgatgtt ttgcaggtat gacatcagag tatctcttca gagcccgggt gtcaagtgcg
     1141 gtggtttgca ttgcggtggt ttgcatatgt tacgagacaa aggacgggac ggccggccc
//
LOCUS       DAOTHD010000817         1199 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_140141_length_1199_cov_3.068182, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000817 DAOTHD010000000
VERSION     DAOTHD010000817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140141_length_1199_cov_3.068182"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..609)
                     /locus_tag="P5219_11665"
     CDS             complement(28..609)
                     /locus_tag="P5219_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27468.1"
                     /translation="MEYGMIRFGSGEDYVVEDVLMCLAKFSPPRPMKGYAVNVSDSLR
                     GGLSFMEGVDGGSGFNGGILLPLSRIYVSLGEDGGSLLIAADTMEERARWISGRVVSR
                     SGRNFNGVIHHTYPGTDWTLVGVTTPGWMLLFDEDAPEAEQDLLDGEVRVAYLPADER
                     LMTFLSPDRSTGEALSFLAEYLLSCLPFREEEA"
     gene            complement(633..1127)
                     /locus_tag="P5219_11670"
     CDS             complement(633..1127)
                     /locus_tag="P5219_11670"
                     /inference="COORDINATES: protein motif:HMM:NF018652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27469.1"
                     /translation="MADGYPFYRWSQWFLPRKGFSQYEIASFSLPGRHSRHNTAYWRR
                     NPVLGLGAGGWGFLGEKRYRNEGSLEEYASSVRSCGGSVAGIEPVSGEKAAREAAVLL
                     LRTKWGISFDAFSHKYGQAMLDNIRDTLRREAPCDCLSETSAGLSLTPKGMRVANALW
                     SLIV"
BASE COUNT          264 a          379 c          323 g          233 t
ORIGIN      
        1 aagcagctta ccgtctgaag agaaccttca tgcctcctct tcccggaagg ggaggcatga
       61 aagcagatat tccgccagga aagaaagggc ttcaccggtg cttctgtcag gagaaaggaa
      121 cgtcataagc cgttcatccg ccggaaggta ggctacccgg acctcaccgt cgaggaggtc
      181 ctgttccgcc tccggggcgt cctcgtcgaa aagaagcatc caacccggcg tcgtgacccc
      241 caccagagtc cagtccgttc cgggataggt atggtgaatc acaccgttga agtttcgccc
      301 cgaacgggaa acgaccctgc cgctgatcca ccgggcccgc tcttccatgg tgtctgccgc
      361 gatgagaagg gatcctccgt cctcgcccag ggaaacatag atcctcgaga gaggaagcag
      421 gatcccaccg ttgaatccgc tccctccatc caccccttcc atgaaggaaa gaccgcccct
      481 caatgaatcg gagacgttta ctgcgtatcc cttcatgggc cgtggggggg aaaatttcgc
      541 aagacacatc agcacgtctt ccaccacgta atcttcaccg gaaccaaagc ggatcattcc
      601 gtattccaca ggagcagccc ctttcgtatt tctcagacaa tcaggctcca gagggcgttt
      661 gcgaccctca ttcctttcgg cgtcagcgaa aggccggcag aagtttccga aaggcagtcg
      721 cagggagctt cccgcctcag ggtgtcccga atattgtcaa gcattgcctg accgtattta
      781 tgcgaaaaag cgtcgaagga aataccccat tttgtcctga gcagaagcac ggcagcctcc
      841 cttgcggcct tctccccgga gaccggctcg atcccggcca cgcttccccc gcaggagcgg
      901 acggaggagg catattcctc caggctgccc tcattccgat accgtttctc cccgagaaac
      961 ccccatccac cggctcccag gcccagaacg ggatttctcc gccagtaagc cgtgttgtgg
     1021 cggctgtggc gtccgggcag agagaaactg gcgatttcat actggctgaa gcctttccgg
     1081 ggaagaaacc actggctcca cctgtagaaa ggatacccgt ccgccattcc ggaagggggg
     1141 gaggaattcc accggcaccc ttccggggtg gatctctccg cctatgatct ggaatggtt
//
LOCUS       DAOTHD010000818         1198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_140346_length_1198_cov_2.559055, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000818 DAOTHD010000000
VERSION     DAOTHD010000818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140346_length_1198_cov_2.559055"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="P5219_11675"
     CDS             complement(<1..633)
                     /locus_tag="P5219_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27470.1"
                     /translation="MKKLPLFLLVLMLVSLTASYGQASSVYVVEPVLVNQPAYSGMNF
                     YVYRPYNLPEGWYVTFDGYPVVRTSSGVWVYGSMSGNGLVRTAYVVGSVDPASFPLAR
                     YPVPAAVSSVNHVQVLSFAGVSLNELLQPVPPSMPSSAGMILPTYMPSWAVNPSFNAI
                     GSWSRLVDRMGILDNPRVPIAWKGDRPQALFAWTGKSWYQMTSSSIDIPER"
BASE COUNT          286 a          301 c          363 g          248 t
ORIGIN      
        1 ccgttccgga atatcaatgg atgaggatgt catctggtac cagctttttc ccgtccatgc
       61 gaagagggct tgcggacggt cccctttcca ggcgatgggc actctgggat tatccagaat
      121 tcccattctg tccacgaggc ggctccagga gccgatagcg ttgaaagatg gattcacggc
      181 ccaggaaggc atgtaggtcg gaaggatcat ccccgcggat gagggcattg aaggcggaac
      241 cggctgcaga agctcgttca gggaaacgcc ggcaaaggaa agaacctgaa cgtggttaac
      301 ggaggaaaca gcggcaggaa cagggtaccg ggcaagaggg aaagatgccg gatcgaccga
      361 gccgacgaca tacgcagtcc ggacgagacc atttccgctc attgagccgt aaacccagac
      421 tcctgaggaa gtccggacaa ccggataacc gtcaaacgtg acgtaccatc cttcggggag
      481 gttgtagggc ctgtagacgt aaaaattcat tccggaatag gccggctgat taacgagaac
      541 aggttcgacg acataaacag aagacgcctg tccgtacgat gcggtaagag agaccagcat
      601 caaaacgaga agaaaaaggg gcagtttttt cataacccaa agtcctcctt tcgaagaatc
      661 tcccccctgt gtggaggaga cgccttcata cccttgcatt atatcgggga aaggccggag
      721 gtgaaagaag atcagtccgt tttcccttca gggggagcag gaggaacgta tgccgggccc
      781 aaggtatcag gtgccgctgc ctggggttcc actatatcct tctctgctct ttcttcgttt
      841 tcttccgatt gcggggctat cactatccgt accgggacaa agggatgcaa tttggcgtta
      901 ccccttcctg aatgagcctg tctatgaaca ggagatgtct caggggcggg aggtatctgc
      961 ggttcttttt ccgcagactc gggaggaagc ggcggattgt acaacggggc cttgccctga
     1021 ggggccggaa gctgttccgg ggaaaggatt gtaaatcctc ctgccgctgc cggcaggacc
     1081 tcaatcctcc ccggccgctt gggatcccag agaggttttc ggccgtcttc cgaaggatat
     1141 gggagaatcc ttgaaagagg gggatatccc gggtgggctt caagcgcttt tttgtacg
//
LOCUS       DAOTHD010000819         1198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_140380_length_1198_cov_2.279965, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000819 DAOTHD010000000
VERSION     DAOTHD010000819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140380_length_1198_cov_2.279965"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="P5219_11680"
     CDS             <1..1113
                     /locus_tag="P5219_11680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRX27471.1"
                     /translation="GIKAACEKSAPSEGELEQGRAELETRRAKGSAKVAGYREMLAAK
                     DGAAFSRYEAGQRSNMEKWEKERAALQGSLNRLLAMETYDLPEDVVLRLDRLTMQFGG
                     LKAVNDLSFDVKKGEIFGLIGPNGAGKTTVFNCITQFYKPASGEIYYRNRENKILSLN
                     SYKVHDVIKTGIVRTFQNVEIVGELSILDNLLVAAHTQYKSNIFSQMLHTRGVRQEEA
                     VLHRKALQALEYCGLAHLREVLPVGMPYGVLKRIELARTLMAGANLIILDEPAAGLND
                     QETVELAGLIRKIQAELGVTIFLVEHDMGLVMDVCEHICAISFGKKLAYGTPAEIQAD
                     PVVQEAYLGTSETDPQEAIGGSHGYQSIRQLCERRI"
BASE COUNT          276 a          342 c          387 g          193 t
ORIGIN      
        1 gggatcaaag cggcctgtga aaagtctgcc ccgtccgaag gcgagctgga gcagggaagg
       61 gcggagctcg aaacccggcg ggccaagggg tccgcaaagg ttgccggcta ccgggagatg
      121 ctggcggcga aagacggtgc ggccttctcc aggtatgagg ccggacagcg ctcgaacatg
      181 gaaaagtggg aaaaggagcg cgcggccctg cagggctcgc tcaaccggct gctggccatg
      241 gagacctacg acctgcccga ggacgtcgtg ttgcggctgg accgcctgac catgcagttc
      301 ggcggcctga aggccgtcaa cgacctgagc ttcgacgtga aaaagggaga gatcttcggc
      361 ctgatcggcc ctaacggcgc gggaaaaacc accgtgttca actgcattac ccagttctac
      421 aagcccgcct ccggagagat ctattaccgc aaccgggaaa ataagattct ctccctcaac
      481 tcgtataagg tacacgacgt gatcaaaacg ggcatcgtgc gcaccttcca gaacgtggag
      541 atcgtggggg agctgtccat tctggacaat ctgctcgtcg ccgcccacac gcaatacaag
      601 agcaacatct tctcgcagat gcttcacacc cggggcgtgc gccaggagga ggcggtgctg
      661 cacagaaagg cgctgcaggc gctggagtac tgcggcctgg cccatctcag ggaggtgctg
      721 cccgtaggca tgccttacgg cgtcttaaag cggatcgaac ttgcccgaac gctcatggcg
      781 ggggccaatc tcatcatcct tgacgagccc gccgcgggcc tcaatgacca ggagaccgtg
      841 gagctggcgg ggctcatccg gaaaatccag gcggagttgg gcgtgaccat cttcctggtg
      901 gaacacgata tgggcctggt tatggacgtg tgcgagcata tctgcgcgat ttccttcggg
      961 aaaaaactgg cctacggcac acccgcggag atccaggcgg accccgtcgt gcaggaggcc
     1021 tacctgggca cctccgaaac ggacccccag gaggccatag gaggtagcca tggctatcaa
     1081 tcaatccgtc agctatgcga acgcagaata tgaaacgtcg aaagaattga agtcggtcga
     1141 tgtgaacgac ggcatgggaa gcgcgacgaa cgggacaacc ggcgaagtgc agacgatg
//
LOCUS       DAOTHD010000820         1198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_140423_length_1198_cov_1.872266, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000820 DAOTHD010000000
VERSION     DAOTHD010000820.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140423_length_1198_cov_1.872266"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="P5219_11685"
     CDS             complement(<1..967)
                     /locus_tag="P5219_11685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRX27472.1"
                     /translation="MESPHTEALSLRGITKVFPGVLANDDVNLSARRGEILAILGENG
                     AGKSTLVKILYGMYQPDSGTVSVNGRAVGIRSPKDALALGIGMIHQHFTLVPVHTVWE
                     NVVLGLDSSSGRPSDGKGAALELEKLGSRYGLEVDPLAVVADLSVGMQQKTEILKALY
                     RSVSILVLDEPTAVLTPGEADNLFAFLREFRAAGNTVLFITHKLGEVMEIADRIEVLR
                     GGRNAGSFLQGEVSERELAARMVGGDIPPVGETPVRPAGRDVLEVRDLWVRGGRGHHA
                     VRGVSFTVRAGEILGIAGVSGNGQEELADCVCGLARQERGEILLDG"
BASE COUNT          221 a          392 c          361 g          224 t
ORIGIN      
        1 gcccgtcgag gaggatctct cccctctcct gtcgggcaag gccgcagaca cagtccgcca
       61 gttcctcctg tccgtttccg ctcactccgg cgatgccgag gatctcgccg gcccgtacgg
      121 tgaaggaaac gccccgtacg gcatgatgcc cccggccgcc cctgacccag aggtccctga
      181 cttccaggac gtcccgtccc gcaggccgga ccggggtctc cccgacaggg gggatgtccc
      241 ctcccaccat gcgggcggca agctcccgct cggacacctc accctgaagg aaggatccgg
      301 cgtttctccc gcccctgagg acttcaatcc tgtcggcaat ctccatgact tctccgagtt
      361 tgtgggtgat gaaaaggaca gtgtttcccg cagcacggaa ttccctgaga aatgcgaaga
      421 gattgtccgc ctccccggga gtcagtacgg cggtgggctc gtcgagaacc aggatgctga
      481 cactgcggta cagggccttc aaaatctccg tcttctgctg catacccacc gaaaggtcgg
      541 ccacaaccgc caaagggtcg acttcaagac cgtatcggct gcccagtttt tccagctcaa
      601 gggcagcccc tttcccgtcg gacggacgcc ctgaagaaga atcaaggccg agcaccacgt
      661 tctcccagac cgtatggacc ggaacgaggg tgaaatgctg gtggatcatg ccgatgccga
      721 gggccagcgc atccttcggc gagcggattc cgactgcccg gccgttcacg gagacagtgc
      781 cgctgtccgg ctggtacatg ccgtacagta tcttcacaag ggtggacttg ccggcgccgt
      841 tctcgcccag aatggccagg atttcacccc ggcgggcgga aagatttaca tcgtcgttgg
      901 cgagcactcc gggaaagacc ttcgtgattc cccggagaga aagggcttcg gtgtgcgggg
      961 attccatggt gtacctcccc ctgcagggtg cgcaggaccc ggagggcttc ccggaccctg
     1021 cggcggttca gactgagcgg ggatcagggc ttctccggat aaccggagag gaagaagagg
     1081 acatcccctc ttttcacagc ttcttcggcg gccttcaccg tctcgcggac ttcgggggcg
     1141 atgtcgtcgg agtaggacac cttgacgatg ccttcggcga tcccggcttc gttaacca
//
LOCUS       DAOTHD010000821         1197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_140557_length_1197_cov_2.500000, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000821 DAOTHD010000000
VERSION     DAOTHD010000821.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140557_length_1197_cov_2.500000"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="P5219_11690"
     CDS             <1..631
                     /locus_tag="P5219_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27473.1"
                     /translation="RDAMVVGTGDLSELALGWCTYGVGDHMSHYNVNASLPKTLIQYL
                     VRWAAASEILGKEVSPVLEAVLGTEISPELVPGSAEDGPSQSTQAVIGPYELQDFNLF
                     YTLRYGYLPSKVAFLAQSAWRDAAAGVWPDILPGRRTEYTLAEIKHWLSVFLRRFFTM
                     SQYKRSCVPDGPKVGSGGSLSPRGDYRAPSDGSSAPWEKDLENIPDGEE"
     gene            complement(674..>1197)
                     /locus_tag="P5219_11695"
     CDS             complement(674..>1197)
                     /locus_tag="P5219_11695"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HRX27474.1"
                     /translation="VEETPPGAGGMLWLPYLNGERTPHRNPNARGVLYGLSSMSDRRH
                     VFRAVMEGITYGLRDSFELVRAATPVKRVIMAGGGAKSAVWRKMTASVMGFPVSIPET
                     EEGGAYGAAMLAALGGGLSPDDVRGWTRILETVEPDDAEKKTYDRGYLQFQALYRDLR
                     ERFDATAHNTGEQ"
BASE COUNT          200 a          398 c          373 g          226 t
ORIGIN      
        1 ccgggatgcc atggtggtgg gcaccggcga cctcagcgag cttgccctcg ggtggtgcac
       61 ctacggcgtg ggggatcaca tgtcccacta caacgtgaat gccagtctcc ccaagaccct
      121 catccagtat ctcgtccggt gggccgccgc aagcgaaatt ctcggcaaag aggtgtcgcc
      181 ggtgctggaa gctgtcctcg gaacggagat cagcccggaa ctcgtcccgg gatcggcgga
      241 ggacggcccc tcccagagta cccaggccgt catcggcccc tatgagctgc aggacttcaa
      301 cctcttctat accctgaggt acggttacct cccgtccaag gtggccttcc tggcacaatc
      361 cgcctggcgg gacgccgccg cgggcgtgtg gcccgacatt ctccccggtc ggaggacgga
      421 gtacaccctt gccgaaataa agcactggct ttccgtcttt ctccgccggt ttttcacaat
      481 gagccagtac aagcgatcct gcgttcccga cgggcccaag gtcggttcgg gaggatccct
      541 ttctccccgg ggcgattacc gggcaccgag cgacggcagc tccgccccgt gggagaagga
      601 cctggagaac atccccgacg gggaggagtg acgggaaaaa ataatcgggg cgggctcaca
      661 gcccgcccct gtcttactgt tcgcccgtgt tgtgtgccgt ggcgtcgaac cgctccctga
      721 ggtcccggta cagggcctgg aactgcagat atcccctgtc gtatgttttc ttttccgcgt
      781 cgtccggttc cacagtttcc aggatccggg tccatcccct cacatcgtcc ggggaaaggc
      841 cgccgccgag ggcggcaagc atggccgcac cgtaggcccc tccttcctcc gtctcgggta
      901 tggagacggg aaaccccatc acggaagcgg tcatttttcg ccagacggcg ctttttgccc
      961 ctcctcccgc catgatgacc cgctttacgg gagtggctgc ccggacgagc tcgaaggaat
     1021 cccgcagtcc gtaggtgatt ccctccatga ccgcccggaa gacatgacgc ctgtcgctca
     1081 tggaggacag gccgtagagc acccccctgg cgttggggtt tctgtggggg gtgcgctcgc
     1141 cgttgaggta gggaagccag agcatgcccc ccgccccggg gggggtctct tcgacgg
//
LOCUS       DAOTHD010000822         1193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_141293_length_1193_cov_2.653779, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000822 DAOTHD010000000
VERSION     DAOTHD010000822.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141293_length_1193_cov_2.653779"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="P5219_11700"
     CDS             <1..649
                     /locus_tag="P5219_11700"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRX27475.1"
                     /translation="LVETVGTVVSLRPGGICAVLTLRAPSFGAELVPGQSVAVCGACL
                     TVTALERESFSVDIMPETLKKTKLRSLRPGKRVNLERALRADGRLDGHIVTGHVDGVV
                     PVREISRGRDGVLLTLGLDGETASLVVPRGSVAVDGVSLTVARLAGTSCTVGLIPSTL
                     EATTLGDLRPGDEVNIETDILGKYVRRLLEAGAFTNKNTGASALSPDSLRDAGWM"
     gene            671..>1193
                     /gene="ribB"
                     /locus_tag="P5219_11705"
     CDS             671..>1193
                     /gene="ribB"
                     /locus_tag="P5219_11705"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HRX27476.1"
                     /translation="MTALCGDGRISGEVFSSIEEAVEDIRNGKMVIVVDDDCRENEGD
                     LIIAASLVTTDAVNFMVRHARGLVCVPLGREIVRRLRLEPMVREGTDKHGTAFLVTVD
                     AREGCTTGISAEERAGTARALADPASAPENFYRPGHMFPLAARQGGVLKRAGHTEAAV
                     DLATLAGLPPAGVI"
BASE COUNT          208 a          353 c          416 g          216 t
ORIGIN      
        1 ccttgtagaa actgtgggaa cggtagtctc cctcaggccc ggcgggatct gcgccgtcct
       61 cacgctcagg gcaccatcgt tcggcgcaga gctggttccc ggccagtccg tggcagtctg
      121 cggcgcctgt cttacggtga ctgctcttga aagagaaagt ttttccgtgg acatcatgcc
      181 ggaaacattg aaaaagacga agctccggtc gctccgcccg gggaaacggg tgaacctgga
      241 acgggccctc cgtgccgacg gcaggcttga cggccatatc gtcacgggtc atgtcgacgg
      301 ggtggtcccg gtacgggaga tttccagggg acgggacggg gttctgctca cccttggcct
      361 ggacggggag acggcatctc tggttgtccc caggggctcc gtggcggtgg acggcgtgag
      421 cctgaccgtg gcccggcttg cgggaacctc ctgtaccgtg gggctgattc cttccacgct
      481 ggaggccacc accctcggag acctccggcc aggtgacgag gtgaacatcg agacggacat
      541 tcttggaaaa tacgtaagga ggcttcttga agcgggggcc ttcaccaaca aaaatacagg
      601 cgcttccgcc ctgtccccgg attcgctccg ggatgccggg tggatgtagg cgcatacggg
      661 aggcattttc atgaccgcac tgtgcgggga cggcaggatc agcggcgaag tgttcagttc
      721 catcgaggag gccgtggagg acatccggaa cggcaaaatg gtgatcgtgg ttgacgacga
      781 ctgcagggag aacgagggcg atctcattat cgccgcttcc cttgtcacca ccgatgccgt
      841 caatttcatg gtccggcacg cccggggact cgtctgtgtt cccctgggaa gggagatcgt
      901 ccggcggctt cggctggagc ccatggtcag ggaaggaacg gacaagcacg gcacagcctt
      961 tctcgtgacg gtggacgcac gggaaggctg taccacgggg atttccgccg aggagcgggc
     1021 gggtaccgcg cgggcccttg cggatccggc atcggctccc gagaactttt accgccccgg
     1081 ccacatgttt cccctggcgg cccggcaggg cggggtgctc aagcgggccg gccacacgga
     1141 ggcggcggtg gacctggcaa ccctggctgg cctgcctccg gcgggggtaa tct
//
LOCUS       DAOTHD010000823         1192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_141491_length_1192_cov_2.568162, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000823 DAOTHD010000000
VERSION     DAOTHD010000823.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141491_length_1192_cov_2.568162"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..>1192
                     /locus_tag="P5219_11710"
     CDS             90..>1192
                     /locus_tag="P5219_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27477.1"
                     /translation="MKRRIISLGGFFSVILLFFLAVILLEGGLTAYFRIRSQLQEFTD
                     RGETFTRVGSHFLKQQVRRGEGSVRGLVRGWSSPDRVNGVLLAAVIEDSTIVSVLKGW
                     LPEGMVLPPEILAPGWKMNDLLDQFGRNLLTGIMTVDGKRVFAAMDLDLNELNVAGTP
                     DILPILSTSTGSVVWTGSEENTSGLASHIRARGLVARKASAEGAWSLLRSHYGERVVL
                     RQEPFLYGLRLSLVYPLSSLVRSAMYGAAVSGSATVAALLALFLIWFVWRRGIYSGIR
                     SITSLAEDMSVRLEDIEGGDHIKAAEAMYSLSRRFAGLKETFVLEMNAFTGNLRNLFQ
                     VISQQQEELTAFNEETEAMNQELENVNNRLVMR"
BASE COUNT          213 a          391 c          358 g          230 t
ORIGIN      
        1 tactccctga caaaagaggg ctggattccc attacccggc cgtcctccgc ctccctgccg
       61 gcctcaggcg ctccggacga ggctgcccca tgaagcggag gattatttcc ctcggcggct
      121 ttttctcggt tatcctcctg ttcttcctgg ccgttatcct cctcgaggga gggctcaccg
      181 cctacttccg gatcaggtcc cagctgcagg aattcaccga ccggggggaa accttcacca
      241 gggtgggctc ccacttcctg aagcagcagg tccgccgggg tgagggctcc gtccggggac
      301 tcgtccgggg ctggtcatcc cccgacaggg tcaacggcgt gctcctggcc gccgttatcg
      361 aagacagcac cattgtttcc gtcctgaagg gatggctgcc ggaaggaatg gtcctccccc
      421 ccgaaatcct cgctcccggc tggaagatga acgatctcct cgaccagttc ggccggaacc
      481 ttctcaccgg catcatgaca gtggacggaa aaagagtgtt cgccgccatg gacctggatc
      541 tcaacgagct gaacgtggcc gggacgccgg acatcctgcc gatcctctcc acctccacgg
      601 gatcggtggt ctggacgggc tccgaagaaa acacatccgg cctggcgtcc cacatccggg
      661 cccggggtct cgtggccagg aaggcaagcg ccgagggagc ctggtccctc cttcgatccc
      721 actacggcga acgggtggtt ctccggcagg aaccctttct gtacgggctc cggctgagcc
      781 tcgtctaccc cctgtcgagc cttgttcgca gcgccatgta cggtgccgcc gtttccggct
      841 cggccacggt ggcagcactg ctggcccttt tcctcatctg gttcgtctgg aggcggggga
      901 tttacagcgg catcaggagc atcacgtccc tggccgagga tatgagcgtc aggctcgagg
      961 atatcgaggg aggggaccat atcaaggccg ccgaggccat gtattccctt tcgaggcgct
     1021 tcgccggcct gaaagagacc ttcgttctgg agatgaacgc cttcaccggc aacctgagga
     1081 accttttcca ggtcatctcc cagcagcagg aggaactcac cgccttcaac gaggagacgg
     1141 aggccatgaa ccaggagctt gagaatgtga acaaccgcct cgtcatgcgg ga
//
LOCUS       DAOTHD010000824         1191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_141629_length_1191_cov_3.369718, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000824 DAOTHD010000000
VERSION     DAOTHD010000824.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141629_length_1191_cov_3.369718"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="P5219_11715"
     CDS             complement(<1..247)
                     /locus_tag="P5219_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRX27478.1"
                     /translation="MEEKKGELLFGKVISLPLVEEIKHSYLDYAMSVIVGRALPDVRD
                     GLKPVQRRILYAMMELGLRAGSSYKKSARVVGETMGKY"
     gene            complement(448..975)
                     /locus_tag="P5219_11720"
     CDS             complement(448..975)
                     /locus_tag="P5219_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRX27479.1"
                     /translation="MTIGENHIIKTILGRRSIRKFTADPVSPEIRDRLLECGCAAPSA
                     HNRRPCVFVVIEDRKVLDDLAEAHDSGKMLRQAPLAIAVCAETAGYPEGDRAWVEDCA
                     AALENILLAARGLGLEGVWLKVMDRHPRNERIRPILAVPDSVEIAGIAALGFGAERKP
                     PYEGFDPSKVRFNRW"
BASE COUNT          236 a          359 c          329 g          267 t
ORIGIN      
        1 ggtacttgcc catggtttct cccacaaccc tggccgactt cttgtaggag gagccggccc
       61 ggaggcccag ttccatcatg gcgtacagaa tacgccgctg cacgggtttg aggccgtcac
      121 ggacgtcggg gagagccctc cccacgatga cgctcatggc gtaatcgaga tagctgtgct
      181 tgatctcctc cacaagggga agagagatca ccttgccgaa aagcagttca cctttttttt
      241 cttccatctg tcttcttcaa cctccaaagc cgatcgccgt cagaaaaagc atgctctcgg
      301 cacaaccctg ttattttacc atgttcgtcc ctgcagatcc ggtattttcg tcgggccgga
      361 atcctcaggc aggggagagc gtcaacctgt gcccggcgac actccctcgg ccgggaaaaa
      421 ctgcggattc aggccgagtc cggatgttca ccagcggttg aagcggacct tcgaagggtc
      481 gaaaccttcg tacggcggtt tcctttccgc cccgaagccg agggcggcaa tgcccgcaat
      541 ttccaccgaa tcaggcacag cgaggatagg acggattctt tcgttccttg ggtgtctgtc
      601 catgacctta agccagaccc cctccagtcc cagtcccctt gcggcgagaa gaatgttctc
      661 cagggcggcg gcgcagtcct ccacccaggc ccggtcgcct tcgggataac cggcagtctc
      721 tgcacatacg gctatggcca ggggagcctg ccggagcatt tttcccgagt cgtgggcctc
      781 cgctagatcg tccaggacct tccggtcctc tatgacaacg aagacgcagg gcctgcggtt
      841 gtgggccgag ggcgcggcac atccgcattc gaggagccgg tcacggatct ccggagaaac
      901 cggatccgcc gtaaatttcc tgatgctccg ccgtcccagg atcgttttga tgatgtggtt
      961 ttcccctatt gtcatgatat acccctccgg gtttattttt tgtcattcta tgctagactt
     1021 gcttcaggca cctctttgga agaacccaga aaaaacgaaa ggagtgagct caatgtcgaa
     1081 aaaagtcgca gttctcgtgg aggagaacgt tcacgatctc gagtactggt atccctttta
     1141 ccgcctggcc gaggcaggtt ttgaacctgt cgccgtggga cccgccgcag g
//
LOCUS       DAOTHD010000825         1191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_141648_length_1191_cov_2.963908, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000825 DAOTHD010000000
VERSION     DAOTHD010000825.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141648_length_1191_cov_2.963908"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..465)
                     /locus_tag="P5219_11725"
     CDS             complement(4..465)
                     /locus_tag="P5219_11725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRX27480.1"
                     /translation="MVNLLAERCLGAAKATGAFAPEELDVLEDVLIEWSLHPGKDYVL
                     LSEWNGGNLAGFLVYGPTPMTRFAFDLYWIAVDPAYQKKGIGRILEEKMCSALLEQSP
                     GAVVRVETAGRDDYLGQRHFYLATGYRECGRIPDFYSEGDDLVLYCKKLGK"
     gene            complement(459..>1191)
                     /locus_tag="P5219_11730"
     CDS             complement(459..>1191)
                     /locus_tag="P5219_11730"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRX27481.1"
                     /translation="KTGTPCTGNSAAVLETCLCKDAVSSLLRGKGIPVPEGKTLLPGG
                     FRSLLDDLPLPLFLKPLREDGSVGIDGNSLVTDRDDLPLRVGEKLKFFPGGIRAEEFL
                     PGMEYSVSCIGNGPYIVPAVSVIDYGKWNAGRPFLDYGSKWDPDSPLYSLTPEPAEGT
                     LKERAVQLASEAGKTLGCRGYFRTDLREKDGTLYVIDINPNPDMGSGGGFLRQCREGG
                     MEKEDVAARIVKLAIENGRGGKEQW"
BASE COUNT          223 a          372 c          339 g          257 t
ORIGIN      
        1 ccgttacttt ccgagttttt tgcagtagag gacaagatcg tcaccctccg aataaaagtc
       61 cggaatgcgt ccgcattccc tgtaacccgt tgccaggtag aaatggcgct ggccaaggta
      121 atcgtcccgc cccgcggttt ccacccgcac gactgcgccg ggagattgct cgagcagggc
      181 cgagcacatc ttttcttcca gtatcctgcc tattcccttc ttctggtacg ccgggtccac
      241 ggcgatccag tacaggtcga aggcgaacct ggtcatgggg gtcgggccgt ataccaggaa
      301 acccgccaga ttgcctccgt tccactcgga gaggaggaca tagtcctttc ccgggtggag
      361 ggaccattca atcagaacat cctcgagaac gtccagttct tcgggggcaa atgccccggt
      421 agcttttgcc gcgcccaggc agcgctcggc cagcagattt accactgttc ttttcctccc
      481 cgcccatttt cgatggcaag cttcacgatg cgggccgcca cgtcttcttt ttccatgccg
      541 ccttcgcggc actgtctcag gaacccgccg cccgatccca tgtccgggtt cgggttgatg
      601 tcgatcacgt agagcgtgcc gtccttttcc ctcaggtcgg tccggaaata tcccctgcag
      661 ccgagagtct ttcccgcttc cgatgccagc tgtaccgctc tctccttcaa tgtcccctcc
      721 gccggttccg gggtaaggct gtagagaggg gagtccgggt cccacttcga accatagtcc
      781 agaaaggggc gcccggcgtt ccatttgccg tagtcgatga cggacacggc gggaacgatg
      841 taggggccgt tcccgatgca ggagacggag tattccattc ccgggagaaa ttcctccgcc
      901 ctgatccctc cggggaagaa cttcagtttt tccccgaccc tcaggggaag gtcgtccctg
      961 tcggtcacaa gagagttccc gtcgatgccc acgcttccgt cctcccgcag aggcttcaga
     1021 aagaggggaa gggggaggtc gtccaggagc gaacggaaac cgccgggaag gagggtcttt
     1081 ccctcaggaa cggggatgcc ctttcctctg agcagggagg aaaccgcatc cttacacaga
     1141 caggtttcga gaactgcagc agaatttcct gtgcagggag tgccggtttt c
//
LOCUS       DAOTHD010000826         1191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_141712_length_1191_cov_2.135563, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000826 DAOTHD010000000
VERSION     DAOTHD010000826.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141712_length_1191_cov_2.135563"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1191
                     /locus_tag="P5219_11735"
     CDS             <1..>1191
                     /locus_tag="P5219_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27482.1"
                     /translation="DGTVAVLDRFGKRIRLFDAAGTLVDEWGEKGRGEDGTFAWPTVI
                     AFAPDGSLYVADEGYSVDANIQHFYRKGEFLGKIKADRKTLGEKGMYKPGFLAVTETG
                     KIVTAGTSEIKDGKPRLLVFSPEGKLLLSRDMEFECPMALLPGEKLVLAKPRPGDDRA
                     DMFAVHDLEGNLVKEWSMWGSDLPALPGEEGIYFRVKYVGSDAEGRVYAYDDSEDGIW
                     IYGPDGRFLQVLPARRTFGIMEGMAVLPGGDVLVKDRPSGYGPGEPSVHRMKNAWPAK
                     VVEQPVRETPPVQPPSDVPPPGGGTTPAVSQTGPDDKGLENELARLKEALRLREEAAA
                     FEKEGNRAGAAAKYRESLAFHNDPKVIAYAEGLERMVASSVSGPTAPKETPKETPKET
                     PKET"
BASE COUNT          257 a          326 c          410 g          198 t
ORIGIN      
        1 ccgatggaac ggtggctgtc cttgaccggt tcggcaaacg gatccgtctc ttcgatgccg
       61 cggggaccct ggtggatgaa tggggggaga agggaagggg agaggacgga accttcgcct
      121 ggccgacggt catagccttt gcccctgacg gatcgctcta cgtggctgac gagggatact
      181 ccgtggatgc gaatatccag catttttacc gcaaggggga gttcctgggc aagataaagg
      241 cggacaggaa aactctcggg gagaagggga tgtacaagcc ggggttcctc gccgttacgg
      301 aaacggggaa aatcgtcacc gcgggaacca gcgaaataaa ggacgggaaa ccgaggctcc
      361 tggttttctc cccggaaggg aaactgcttc tctcccggga tatggagttc gaatgcccca
      421 tggcgcttct tccgggggaa aagctcgttc tggcgaaacc ccggccgggt gatgaccggg
      481 ccgatatgtt tgctgtgcat gacctggaag ggaacctcgt gaaggaatgg tccatgtggg
      541 ggagtgacct gcctgccctc ccgggggaag aggggatcta tttccgggtg aagtacgtcg
      601 ggtccgacgc ggaaggacgg gtgtatgcct acgatgattc cgaggacgga atctggatct
      661 acggcccgga cggcaggttt ctccaggttc ttcccgcaag gaggaccttc ggcatcatgg
      721 agggcatggc ggttctgccc ggcggagacg tgcttgtcaa ggaccgtccc agcggatacg
      781 ggccgggaga accgtcggtg cacaggatga aaaatgcctg gccggcgaag gtagttgaac
      841 agcccgtccg ggaaaccccg cccgtccagc ctccttcgga tgtccctccc ccggggggcg
      901 ggaccactcc tgcggtttcg caaaccggtc cggatgacaa aggccttgaa aacgaacttg
      961 cccgcctgaa ggaagccctc cggctgcggg aagaggcggc ggccttcgag aaggagggga
     1021 accgcgccgg tgcggcggcg aaatacaggg aaagcctcgc attccacaat gaccccaagg
     1081 taatagccta cgccgagggg ctcgagcgga tggtcgcctc ctcggtctcc ggccctacgg
     1141 ctccgaaaga gacaccgaaa gagacgccga aagagacacc gaaagagacg c
//
LOCUS       DAOTHD010000827         1188 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_142188_length_1188_cov_3.072374, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000827 DAOTHD010000000
VERSION     DAOTHD010000827.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142188_length_1188_cov_3.072374"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..115)
                     /locus_tag="P5219_11740"
     CDS             complement(<1..115)
                     /locus_tag="P5219_11740"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HRX27483.1"
                     /translation="MDKGKLQHFREKIDTLDKQIAGLIAERAETAREIGRAK"
     gene            489..962
                     /gene="ilvN"
                     /locus_tag="P5219_11745"
     CDS             489..962
                     /gene="ilvN"
                     /locus_tag="P5219_11745"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRX27484.1"
                     /translation="MERTIGVVTEEGRGVFSRISGLMERRGFQVRGVTAGATQRPGMA
                     RYTLVIGGDERQAGQAVRQIRKMVETVTVEDLSAGGCVERCMMLLKFAPSPEEREALF
                     EVMKGYPHSVSEDCGSSLIFEMMGPGVLLDDCLENVRGLGLVESVKSGPLALGTN"
     gene            1003..>1188
                     /locus_tag="P5219_11750"
     CDS             1003..>1188
                     /locus_tag="P5219_11750"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870338.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRX27485.1"
                     /translation="MATVLYDRDANMKVLEGKTVAVLGYGSQGHAHAQNLRDSGVSVV
                     VGLHEGSRSRATAERDGF"
BASE COUNT          230 a          336 c          387 g          235 t
ORIGIN      
        1 ccttggccct gccgatctcc cgggcggtct ccgccctctc ggcgatgagc ccggcgatct
       61 gtttgtccag agtgtcgatc ttctcccgga aatgctgcag ctttcccttg tccatgaaaa
      121 ctccctccag cccttgtatt tttcgaattt tcttttattt ttgagtctct tgacagtccg
      181 gaaaaacccc tgtaggatta cccacaattt ccggcacccg cccagtaatt taagcacagc
      241 gccgggaaac atggtatcac acaacgcaga agcgaaggag ggagttccgg tgaagaaaat
      301 gacccgtacc gtgtgttcgg ccagtgccgt ttccgcaatt atcgccgtca ttattattcg
      361 ctgcggggcc cctcccggag cgcttctcgt cgtgtagccg gacgaaggca cttccgaggg
      421 acccccgcac attcagcggg ggtccctttt tttatccccg catttttcac gaaggaggag
      481 agaggcgcat ggaacggacc ataggcgtag tgacggagga aggacggggg gtattttccc
      541 gcatttcggg gctcatggag cgcaggggat tccaggtccg gggcgtgacc gccggggcga
      601 cccagcgccc gggaatggcc cggtacaccc tggtgatcgg cggggatgaa cggcaggcgg
      661 gccaggccgt gcgccagatc cggaagatgg tggagaccgt gacggtggag gatctgagcg
      721 ccggcgggtg cgtggagcgg tgcatgatgc tcctgaagtt cgctccgtcg ccggaggagc
      781 gggaggccct tttcgaggtc atgaagggct accctcattc ggtgtcggag gactgcggct
      841 catccctcat cttcgagatg atgggccccg gcgtcctgct ggacgactgc cttgaaaacg
      901 tgcggggcct cggcctcgtg gaatcggtga aaagcggccc cctggccctt ggaacgaact
      961 gacggcagca cacaatctca ttacgcgaag gagctggaaa agatggcaac agtactgtac
     1021 gacagggatg cgaacatgaa ggttctcgag ggaaaaacgg tggccgtgct cgggtacgga
     1081 agccaggggc acgcccatgc ccagaacctg cgcgacagcg gggtctccgt ggtggtgggg
     1141 cttcacgagg gcagcagatc ccgggctact gccgagagag acggcttt
//
LOCUS       DAOTHD010000828         1186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_142598_length_1186_cov_2.422635, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000828 DAOTHD010000000
VERSION     DAOTHD010000828.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142598_length_1186_cov_2.422635"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..1000
                     /locus_tag="P5219_11755"
     CDS             68..1000
                     /locus_tag="P5219_11755"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRX27486.1"
                     /translation="MALHRETPLLESVPLGAVSGRRVFLKMENMQPAGSFKLRGIGRV
                     CEEAANAGATRFISPSGGNAGYAAAWSGRELGVCTTVIVPVTTSEEAKKAIASLGAEV
                     IVSGKSWKESNELALKMSGEDPKSKYIHAFDDPVMWDGHGTLIDEAAKQGPKPDAVVL
                     SVGGGGLLCGVVAGMDRNGWGDVPVLACETEGAASFAAAVAAGEIVELEGITSVATSL
                     GARAVAPMALELTRNHRIIPYVAADEAAVSACGRFLDDHRVLVEPACGVSLSAIYDNT
                     PLLGDAGTVLVVVCGGIGISAAKLRDLETRFGLS"
BASE COUNT          215 a          369 c          375 g          227 t
ORIGIN      
        1 gaacgagagc gtcttctgga tcggcccggc agtcatggcg aaatacgccc tcacctggca
       61 gtaagccatg gccctgcacc gggaaacccc tctcctggaa tccgtccccc tgggcgccgt
      121 ctcggggcgg agggtgttcc tgaaaatgga gaacatgcag cccgccggat ccttcaagct
      181 gcgggggatc ggccgtgtct gcgaggaagc ggccaatgcc ggggcgacgc ggttcatctc
      241 cccctccggc ggaaacgccg gctacgccgc cgcatggtcc gggagggagc ttggcgtctg
      301 caccaccgtc atcgtcccgg taaccacgtc cgaagaggcg aagaaagcca tcgcctccct
      361 gggagccgag gttatcgtct ccgggaaaag ctggaaggaa tccaacgagc tggccctgaa
      421 gatgtccggg gaagatccga agtcgaagta catccatgcc tttgacgacc ccgtcatgtg
      481 ggacggccac ggtaccctca tcgacgaagc ggcgaaacag ggaccgaagc ccgacgcagt
      541 cgtgctgtca gtggggggcg gcggcctgct ctgcggcgtg gtggccggaa tggaccgcaa
      601 cggctggggg gatgtaccgg tgctcgcctg cgaaaccgag ggagcggcct cctttgccgc
      661 cgccgttgcg gcgggggaga tcgtggaact ggagggcata acatccgtgg ccacttcgct
      721 gggggcccgt gcagtggccc cgatggctct cgagctgacc cggaaccacc ggatcatacc
      781 ctacgtcgct gccgatgaag cggccgtctc tgcctgcggg cggttcctcg acgaccaccg
      841 ggtcctcgtg gagcccgcct gcggtgtctc cctttccgcc atctacgata atacgcccct
      901 cctcggagac gccggcaccg ttctcgtggt ggtatgcgga ggcatcggga tcagcgcggc
      961 caagctcagg gacctggaga ccaggttcgg cctttcctga gagctgaaat atccgtccca
     1021 taacggcggg gttgtctccg gaacaccgga gagactccgc cgtttttttc gtttttcgaa
     1081 ttcatccggc ctgaaaatat ttcaaaattt ggatttttat aatattatga tacccgttcc
     1141 attgtcatct ctctttcccg gggtacgccc cgatttttct caagga
//
LOCUS       DAOTHD010000829         1184 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_143012_length_1184_cov_2.012400, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000829 DAOTHD010000000
VERSION     DAOTHD010000829.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143012_length_1184_cov_2.012400"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="P5219_11760"
     CDS             complement(<1..644)
                     /locus_tag="P5219_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRX27487.1"
                     /translation="MKICVTGAGGFIGSHLAEKLAEQGHEVRAFLRYNSKNSWGWLEE
                     SEYAKEMDIVSGDIRDFDLVRQAVHGCDRVFHLAALIGIPYSYVSPLAYVKTNVEGTY
                     NVLQAARDCGVERVIHTSTSEVYGTARYVPIDEGHPITPQSPYSATKASADYLALSYY
                     RSFGLPVTVVRPFNTYGPRQSARAIIPTIISQLLDGKEEISLGNLSPTRDLTFV"
     gene            complement(767..1117)
                     /locus_tag="P5219_11765"
     CDS             complement(767..1117)
                     /locus_tag="P5219_11765"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HRX27488.1"
                     /translation="MEILRSAVSQVLPDKAPAGAAVGKLSEVPPLKKTAEPPVKPVSE
                     KQVQDAVDQANKLAVAFDRSLKYEYKKEADVYQVSVIDTSKDEVVRKIPPDEVVRFME
                     NIRELFGALLDVNA"
BASE COUNT          252 a          326 c          340 g          266 t
ORIGIN      
        1 tccacgaagg tcaggtcccg ggttggggaa aggttcccca gggagatttc ctcttttccg
       61 tcgaggagct ggctgatgat ggtggggatg atggcccgcg ccgactgcct cgggccgtag
      121 gtgttaaagg gccggaccac cgtcacggga aggccgaagg agcgataata ggagagtgcg
      181 agataatccg ccgaggcctt ggtggccgaa tagggggact ggggagttat ggggtgtcct
      241 tcgtcaatgg ggacatatcg ggccgttccg tagacttcgc tggtggaggt gtggatcacc
      301 cgctcaactc cgcaatcccg ggcggcctgg aggacgttgt aggtcccctc cacgttggtc
      361 ttcacatagg ccaggggaga aacgtaggaa tagggtatgc cgatgagggc cgctaggtga
      421 aagaccctat cacagccatg aacggcctgc cgaacgaggt cgaaatcacg gatatctccc
      481 gaaacgatgt ccatttcttt ggcgtattcg gactcctcaa gccatcccca ggagtttttc
      541 gagttgtacc gaagaaaggc ccgaacttcg tgcccctgct cggccagttt ttcggccagg
      601 tgtgaaccaa tgaacccccc ggctcctgtg acacatatct tcatgcggct tcccccataa
      661 tggactgttc cggaaaattt taatcccatt ccagtatcgt ccgtttgagg gaaaaaataa
      721 agaggcggcc gcttctgcgg gcgcctctgg cagtacatcg caaatatcag gcattcacat
      781 cgagaagggc gccgaaaagc tccctgatat tctccatgaa acggaccact tcgtcgggcg
      841 gaatcttccg gaccacttcg tccttcgagg tatcgatgac cgacacctgg tagacatccg
      901 cctccttctt gtactcgtac ttcaggctcc ggtcgaaggc caccgccagc ttgtttgcct
      961 ggtcgacggc atcctgcacc tgcttttccg ataccggttt taccggaggc tccgcagtct
     1021 ttttcagcgg aggaacttcg gaaagtttac caacggccgc gcctgccggg gccttgtccg
     1081 gtaaaacctg tgaaacggca gaacgaagta tttccattat tgcacctcct tgtctctagt
     1141 aaaaagaggg gagaaaactc ccctctttct gtagcgtgct agag
//
LOCUS       DAOTHD010000830         1182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_143270_length_1182_cov_3.261757, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000830 DAOTHD010000000
VERSION     DAOTHD010000830.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143270_length_1182_cov_3.261757"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            373..>1182
                     /locus_tag="P5219_11770"
     CDS             373..>1182
                     /locus_tag="P5219_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27489.1"
                     /translation="MQNSVFQISGETVEYLNSFLSDPANGAVSDLRRIVAKFGSVSEI
                     NERAREAGKVENLVKKLERINSPHVKDLEWLAEIRDGGKLVSLSDYRARINPGRPSWD
                     YDHSRAATLEISALQYFPWLLAEVRQCVEKGEVLPGRFIRVRKMKEQEADGGDLPAVH
                     AALKILGASCVETLDTKGVDGSNVHLGGPETITGYFGGIGQPNSHPLRWVEEALYYYV
                     NYGVDEILNINPGTVFLGYLLYRMGVDIRFKISVFMGNDNPYAVLWTLLAAR"
BASE COUNT          258 a          345 c          337 g          242 t
ORIGIN      
        1 accatccctt gtcccgcaaa taccatcaaa atccataaag tataacacca aagattcgaa
       61 cattctgtca gcttccgtgc tccatcgcca atcatcccac cggcatttcg gggttgacag
      121 aaccggaatg ctcgggtact atgctcccta aactttttca ccagccggga aaggaggata
      181 cgcacgtgac acggatttct cagccagtac tttcagccgt tcttaacgcc atgatgatgc
      241 gcagcgaccc tcctggagcc gggggcgcct agtttcgcgc gtcttttgag cttcagccgg
      301 gggtcttcgg aaacatcgaa ggcccccggc tttttttatg gaaaaaaaca cactacgagg
      361 aggttttttg caatgcagaa cagcgtgttc cagatcagcg gagaaacagt tgagtacctt
      421 aattctttcc tttccgatcc cgcaaacggg gcggtttctg atctcaggcg gatcgtggcg
      481 aagttcggct cggtgagcga gatcaacgag cgggcccggg aagcgggaaa agtggaaaat
      541 ctcgtcaaaa aactggaacg gatcaattct ccccatgtaa aggaccttga atggctcgcg
      601 gagatccgcg acggggggaa gctggtctct ctgtcggact accgggcacg gatcaacccc
      661 ggccggcctt catgggacta tgaccattcc cgggcggcga ccctggagat cagcgctctg
      721 cagtatttcc cctggcttct cgccgaagtt cgccagtgcg tggaaaaggg ggaggtgctc
      781 cccggcaggt tcatccgggt acggaagatg aaggagcagg aagcggacgg gggcgacctt
      841 cccgccgtgc acgccgccct gaaaattctc ggcgcctcct gcgtggaaac cctggacacc
      901 aagggcgtgg acggttcaaa cgtccacctc ggcgggcccg agaccatcac cggctacttc
      961 ggcggcatcg gccagcccaa cagccatccc ttgcggtggg tggaggaagc gctgtactac
     1021 tacgtgaact acggtgtgga cgagatcctc aacatcaatc ccgggacggt gttcctgggc
     1081 tacctgctct accgcatggg cgtggacatc cggttcaaaa tctccgtttt catggggaac
     1141 gacaacccct atgcggttct ctggaccctg cttgcggccc gg
//
LOCUS       DAOTHD010000831         1181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_143463_length_1181_cov_3.316163, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000831 DAOTHD010000000
VERSION     DAOTHD010000831.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143463_length_1181_cov_3.316163"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="P5219_11775"
     CDS             complement(<1..618)
                     /locus_tag="P5219_11775"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRX27490.1"
                     /translation="MARIIKERDLMDNHGISSEWLGTYIEGFRGKDGTLHPLLEVKRV
                     HSMKVRDNCALIADGLGWTGSRRDLALTAAVLHDAGRFLQFADYGTFFDGASVDHGDL
                     GRRVLEEAFPWERLEGEGVKETVLEAVRLHNKKALPAGVPPHQRPVAEIVRDSDKIDV
                     FRLVRRHVEENRVNDLLPRLDVNGGYSGELIRELEEDGQGSYRNVR"
     gene            733..>1181
                     /locus_tag="P5219_11780"
     CDS             733..>1181
                     /locus_tag="P5219_11780"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRX27491.1"
                     /translation="MKMKLHRSIPLFLLAAALLALSAPFEASAWRGVQKWSYDAGNSV
                     TGSPAAVNGTIVIGDSEGRLVALDKATGELRWTFSAGGTINGTPALLGSTLFTGGADG
                     SLFAVDGATGREVWHFTPREKYTPGAIWSSPAAGNGRVYFNSADGKV"
BASE COUNT          229 a          380 c          337 g          235 t
ORIGIN      
        1 gcggacattc cggtaggacc cctgcccgtc ttcctcaagc tcccggataa gttcaccgga
       61 ataccctccg ttcacgtcca gcctggggag gaggtcattg accctgtttt cctccacgtg
      121 gcggcggacc aggcggaaaa cgtcgatctt gtcgctgtcc ctgacgattt ccgccacggg
      181 ccgctgatgg ggcggaacac cggcgggaag ggctttttta ttgtgcagcc ggaccgcttc
      241 cagaacggtc tctttcactc cctctccttc aagccgctcc cagggaaacg cctcttcgag
      301 caccctgcgt cccaggtccc cgtgatccac cgaagcaccg tcgaagaagg tcccgtagtc
      361 ggcgaactgg agaaaccgtc cggcatcgtg cagaaccgcc gccgtcaggg ccaggtcccg
      421 acgggagcct gtccacccca ggccgtcggc gatcagggca cagttgtccc tgaccttcat
      481 gctgtgaacc cgtttaacct cgagaagggg gtggagggtt ccgtcttttc cccggaaacc
      541 ttcgatatag gtcccgagcc attccgaaga aatgccgtga ttgtccatga ggtcacgctc
      601 ctttattatc cgggccattc tatccctctc agccatcctt ttcaatcgcc gggggttccc
      661 ggccgttgaa aacaccccgg aatggtataa ttaatccggc tgcaaaactg aaaaacaagt
      721 gaaaagaggt ggatgaaaat gaaattgcat cgatcaattc cgctgttcct tctggcggcc
      781 gcattgcttg ccctatcggc ccccttcgag gcatcggcct ggcggggcgt tcagaaatgg
      841 agctacgacg ccggaaacag cgtcactgga tcccctgccg cggtgaacgg cacaatcgtc
      901 atcggtgaca gcgaagggcg ccttgtcgct ctcgacaagg ccacagggga gctccggtgg
      961 actttttctg cgggcggcac catcaatggg acccccgctc ttctcggctc cacgctgttt
     1021 acgggaggag ccgacggatc cctcttcgcc gtcgacgggg caacgggacg ggaggtctgg
     1081 catttcaccc cccgggaaaa gtacaccccc ggcgccatct ggtcatcccc ggcggcggga
     1141 aacggacggg tctacttcaa cagcgccgac ggcaaggtct a
//
LOCUS       DAOTHD010000832         1181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_143464_length_1181_cov_3.284192, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000832 DAOTHD010000000
VERSION     DAOTHD010000832.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143464_length_1181_cov_3.284192"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="P5219_11785"
     CDS             complement(<1..791)
                     /locus_tag="P5219_11785"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HRX27492.1"
                     /translation="MEFRRVRLARSTTTNPWDNLAREEYLVNLCGDDEAILYLWQNAH
                     TVVVGRNQNPWAECRLDLLESEGGRLARRSTGGGAVYHDLGNLNYSFILPRRAYSMER
                     QLGTVLGALRTLGISAEFSGRNDLVIGGRKFSGNAYQLTRHKGLHHGTLLLSADMAKL
                     SRYLNVDPEKLKTKGVASVASRVMNLRDALPGLSLDDLLDALEKEFLDRYRAPEVLRE
                     TKEPEGEEYASLAERYRSREWRLGRSPDCAVTVRRRFPWGGMEIRL"
BASE COUNT          210 a          341 c          403 g          227 t
ORIGIN      
        1 agccggatct ccatccctcc ccagggaaac cgcctgcgca ccgtcacggc acagtcaggc
       61 gaccggccga ggcgccattc ccggctccgg taccgttccg ccagcgaggc gtattcttcc
      121 ccttccggct ccttcgtctc ccggagaact tcaggcgccc tgtaccggtc aaggaactcc
      181 ttttcaaggg catccagaag gtcgtccagg gagaggccgg ggagagcgtc ccggaggttc
      241 attaccctgg aagccaccga agccacgccc ttggtcttca gtttttcggg gtccacgttc
      301 aggtaccttg agagttttgc catgtcggcg gagaggagga gggtgccgtg atggagccct
      361 ttgtgacggg tgagctggta ggcgttgccg gagaacttcc tgccgccgat gaccaggtcg
      421 ttcctgccgg agaattcggc ggagatgccc agggtgcgga gggctccgag aaccgttccg
      481 agctgccgtt ccatgctgta ggcccgccgg ggcagaatga aggaatagtt gaggtttccc
      541 aggtcgtggt agactgcccc gccgccggtg gagcgccggg cgagcctgcc cccctccgac
      601 tcgaggaggt cgaggcggca ttccgcccag gggttctggt tccgccccac caccacggtg
      661 tgggcgttct gccagaggta gaggatggcc tcgtcgtctc cgcagaggtt cacgaggtat
      721 tcttcccggg cgaggttgtc ccaggggttt gttgttgtgg atcgggccag ccggactctt
      781 cggaattcca cggtcatcct cctttgttct ccggaagggg gaacccccag cccgggaaag
      841 cgggtgatca gggacggcga aaagcggaca agagccttgt ttccgccatg atatccccct
      901 ttggcgaagg ggtcaatggc cgctttttgc caacggcaga agcatatcac aaaataccat
      961 tgaggggtat atgaagaggt ataatgtccg ggaaattttt cagccgggga tgcagcccgg
     1021 ccgtcattgg agagggtgtc atgacggaac ggcggagacg gaaggaatgg aacggcatcg
     1081 aacggccgga cggtacccgg atcaggtggt cggcggggga aggggaacgt cccatccttc
     1141 tcgtccccgg cttcggcggc gacggggact gttgggggac g
//
LOCUS       DAOTHD010000833         1181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_143490_length_1181_cov_2.745115, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000833 DAOTHD010000000
VERSION     DAOTHD010000833.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143490_length_1181_cov_2.745115"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="P5219_11790"
     CDS             <1..584
                     /locus_tag="P5219_11790"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRX27493.1"
                     /translation="KIDLNMIEHALGASEMMRRLTPSAPPFFSSNGSPLFFRMPYTEE
                     ILETFLNRTGADPTLFTLEVTESLLIENLGEVSRKLNRLKEIGISIALDDFGTGYSSL
                     QYINQLPFDYVKLDKSFVARLFDSEKDMRLLRTIINMAGELRLEVIAEGVETQEQLEW
                     LTRAGCGKVQGYLFSKPVPWEDVKRMMGGEERQ"
     gene            581..>1181
                     /locus_tag="P5219_11795"
     CDS             581..>1181
                     /locus_tag="P5219_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27494.1"
                     /translation="MMGSIKKKLPLALLLAAVLFLFPAPLPAAGSTLRIPVLFLGDLH
                     SQLLPVSLKANKTTVLFGGLVNGASLLAKERAAEPSALILQGGDAVSGIMWLHFAGEP
                     EFATLEAAGVQAFLLGNHEFNYGTDHLKKGLGRTSISALASNLYFDDQDLAERVSRYV
                     ILESAKTKVGVFGIASPNLFAQASPGPGVHLNRNVEAVSV"
BASE COUNT          250 a          368 c          333 g          230 t
ORIGIN      
        1 ggaaaatcga cctgaacatg atagagcacg cccttggcgc ctccgagatg atgcggaggc
       61 tcaccccctc cgccccgccc ttcttctcgt ccaacgggtc gccccttttc ttccggatgc
      121 cctacacgga ggagatcctg gagaccttcc tgaacagaac cggggccgat ccgaccctct
      181 ttaccctgga agtcaccgag agcctgctga tcgaaaacct cggggaggtg tccagaaaac
      241 tcaacaggct gaaagaaatc ggaatcagca tcgccctcga cgatttcggc accggctatt
      301 cgtccctcca gtacatcaac cagcttccct tcgactacgt gaagctggat aaatccttcg
      361 tcgccaggct tttcgactcc gagaaggaca tgcgcctgct ccggacgatc atcaacatgg
      421 ccggggagct ccggctggag gtcatcgccg agggagtgga aacgcaggag cagcttgaat
      481 ggctcaccag ggcggggtgc gggaaagttc agggatacct cttctcgaag cccgttccct
      541 gggaggacgt gaaaagaatg atgggaggcg aagaaagaca gtgatgggtt caataaaaaa
      601 gaaattgccc ctcgcgctcc ttcttgcggc agtacttttt ctttttcccg cccctcttcc
      661 ggcagcggga tcaaccctac ggatacccgt cctctttctg ggggacctcc acagccagct
      721 cctgcccgtc tccctcaagg cgaacaagac cacggtcctc ttcggcgggc tggtcaacgg
      781 cgcgtctctc ctggcaaagg agcgggcggc ggaaccttct gccctcatac tccagggagg
      841 ggacgccgtc tcgggcatca tgtggcttca ttttgccggg gagcccgagt tcgccaccct
      901 ggaagccgca ggtgtacagg ccttcctcct gggaaaccat gagttcaact acggcactga
      961 ccacctgaaa aaagggctgg gaaggacgtc catatccgcc ctggcatcaa acctgtattt
     1021 tgacgaccag gatctggcgg aacgggtttc cagatacgtg atcctggagt cggcgaagac
     1081 gaaagtgggc gtcttcggca tcgcctctcc caatctcttc gcccaggcga gcccggggcc
     1141 gggagtgcat ctcaaccgga acgtggaagc cgtctccgtc a
//
LOCUS       DAOTHD010000834         1180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_143696_length_1180_cov_2.536889, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000834 DAOTHD010000000
VERSION     DAOTHD010000834.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143696_length_1180_cov_2.536889"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..602
                     /gene="dut"
                     /locus_tag="P5219_11800"
     CDS             156..602
                     /gene="dut"
                     /locus_tag="P5219_11800"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRX27495.1"
                     /translation="MEVPVKVKIVREGRAKELPLPEYATPFSAGVDLRAADDCVLLPG
                     EWTAVPTGLRIELPEGYEGQVRPRSGLAARHGVTVLNAPGTIDSDYRGEIRVLLINHG
                     KESFTISAGDRIAQLILAPVSRFSWSEEALAGSERGEGGFGSTGRS"
     misc_binding    640..869
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            916..>1180
                     /locus_tag="P5219_11805"
     CDS             916..>1180
                     /locus_tag="P5219_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRX27496.1"
                     /translation="MYELKEPGGKSVCLSSGTAGEALEKLGLKGGAVAAMLNGRAVDL
                     TAEIGESGDITPIAADSEEGLDILRHSAAHLMAQAVSELYPGTR"
BASE COUNT          260 a          330 c          398 g          192 t
ORIGIN      
        1 ggaagaaact atcgccgccc ttgcggctgc ggcaaaagct aatccttcac ccgacaggga
       61 ccgcccttct cacggaggcg ggaggcctga ccggggagac aggagaggag gcggcccgcg
      121 ccgtcattct tccggaggaa gggaataggc ctcccatgga ggttcctgta aaggtaaaga
      181 tcgtccggga ggggagggca aaggagcttc ccctcccgga atatgccacc cctttttccg
      241 cgggcgtcga cctgagggcg gccgatgact gcgttcttct tcccggtgaa tggaccgccg
      301 ttcccacggg gctcaggatc gagcttccgg agggatacga aggccaagtc aggcccagga
      361 gcgggcttgc ggccaggcac ggagtcacgg tgctgaacgc tccagggacc atcgacagcg
      421 attacagggg cgagatacgg gtactcctca tcaaccacgg aaaggaatcc ttcaccatct
      481 ccgccggcga caggatcgcc cagctcatcc tggccccggt gtccaggttt tcctggagcg
      541 aggaggccct ggccggctcg gaacgggggg aaggcggttt cggaagcacc ggcaggagtt
      601 gacagaaacg ccttttcggg gtataagaca caaaaacagg tttatgcgat gatggggaga
      661 gtagaagaat ccctccgaac cagagagggg agcccgaggc tggaaggttt cccgcggaac
      721 ttcttcgaaa accgcccctg agcgggcgcc gacaaacctt cgggaacaag gcgccggggt
      781 tcgcccctta cagcggtcta cgagcgccgt ccttactatg aaagggcgga attggagtgg
      841 aaccacggca tgcccacgcc gtctccagat ggagatggcg tgggcttttt tcattttttt
      901 gaggaggaga gaatcatgta cgaactgaag gaaccgggag gaaaaagcgt gtgcctttcg
      961 tcgggaacgg cgggggaagc actggaaaaa ctcggcctca aaggcggagc cgtagcggcg
     1021 atgctgaatg gaagggcagt ggacctgacc gcagaaatcg gggagagcgg cgacatcact
     1081 cccatagcgg ccgactcgga agaggggctg gacattctcc gccactcggc ggcccatctc
     1141 atggcccagg cggtgtcgga actctatccc ggcacgcggt
//
LOCUS       DAOTHD010000835         1176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_144515_length_1176_cov_1.676182, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000835 DAOTHD010000000
VERSION     DAOTHD010000835.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144515_length_1176_cov_1.676182"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..632)
                     /locus_tag="P5219_11810"
     CDS             complement(210..632)
                     /locus_tag="P5219_11810"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRX27497.1"
                     /translation="MSRTKIFLDTNILVYTMDGHDSEKQARARAIVRSVVEDQIPVIS
                     TQVMQEFYSASTTKLKVDAMIAKNIVHNLGNMEVVPVDLTTIEQGIDISVLFRLSFWD
                     GLIIAAAEQARCAMVFSEDLNDGQTVRGVRIVNPFLHS"
     gene            complement(625..849)
                     /locus_tag="P5219_11815"
     CDS             complement(625..849)
                     /locus_tag="P5219_11815"
                     /inference="COORDINATES: protein motif:HMM:NF039417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HRX27498.1"
                     /translation="MRNITLSIDKEILKRGREYAKRHNISFNSLVRRLVEQTVTTDSS
                     QWLEDTFSLMDRAGASSGGETWTRDELHRV"
BASE COUNT          254 a          324 c          306 g          292 t
ORIGIN      
        1 acaagacgtt ccgcgacaag aaggacggct gcatcaaggt cgtcctgaag ccctagcggg
       61 aaagcaaccg gaatgacaac gcgggccggg gatcatctcc ccggcccgcg ttctttataa
      121 aacccgtttt cgtccgctgt gcggaatttc attccccggc gttcatttcg aaaatccaaa
      181 gggaacctct gccgggaaag gcggaaaact caggaatgaa ggaaaggatt gactatccgt
      241 actccccgga ctgtctgccc gtcgttcaga tcttcagaaa aaaccatggc gcaccgtgcc
      301 tgctctgccg ccgcgattat caggccgtcc cagaacgaca gccggaaaag aacgctgatg
      361 tcgattccct gttcgatggt ggtcaggtca acgggaacca cttccatatt gccgaggttg
      421 tgcacgatat tcttcgcgat catggcgtca accttcagct tcgtcgtgga ggcgctgtag
      481 aactcctgca tcacctgggt ggagatgacg gggatctggt cttccaccac gcttctcact
      541 atggccctgg ccctggcctg tttctcgctg tcatggccgt ccatagtgta caccagaata
      601 ttcgtgtcca gaaatatctt ggtcctagac acggtgcagt tcgtcccttg tccatgtttc
      661 gccgccggag gaggcccccg ccctgtccat gagggagaag gtatcctcca gccattggga
      721 ggagtccgtc gtgacagtct gttcgaccag gcgccggacg agggagttga aggagatatt
      781 gtgccgcttc gcgtactctc ttccgcgttt gagaatttcc ttgtcaatcg aaagggttat
      841 gttccgcata ttcccacctc ctttttacac ataatatgtg cacgcatatc tcttgtcaag
      901 attttgaaaa cgaagccccg ggcaacatgc aacgcgggcc ggggagatga tccccggccc
      961 gcttattttg tctgcgctct tttgccgtcg gtttttcttc ggacactcaa tccctacttc
     1021 ttcaggctat tgagccacgg aaatgctatc ttttcgaatt cctcagtctt gctgaaaaga
     1081 agatggtgtt ctccaccctg tattttaata aatttgtagg gggcaccaga tttgtccagc
     1141 agttgagcca gggtactgga ctgggaaatc ggaaca
//
LOCUS       DAOTHD010000836         1175 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_144618_length_1175_cov_2.553571, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000836 DAOTHD010000000
VERSION     DAOTHD010000836.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144618_length_1175_cov_2.553571"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1175)
                     /locus_tag="P5219_11820"
     CDS             complement(<1..>1175)
                     /locus_tag="P5219_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27499.1"
                     /translation="KPSCSPESIRIVLEEDRKDLEEAKKALREAAAAVEEIVGRNGAD
                     EKTLQPAVRTRAGALIYPSGTPERRRMISEYREYLESLRSRQTPPGVRISGPDTVGEG
                     MEEFFTVVLDRELRSARYDWKFASGGDGEALEGDTTSRIRWTPSSPGKKVLEVHVLAD
                     LPGADWVRAVHPVTVLSAADTPRPRLRLTCPVDSFRAGEIVPLTAETEEFGLGGEGFE
                     WYIWFADGRRIASTAERTISFDGTGYEGRTVEIRVEARTKNRFVSASALSLEVLPPGG
                     ADGDLRVLLEPDITEAAAGTPLRFSGTAFPRKGGGTLKYQWSVNGEFAGDGDALHFDT
                     APWEGKTAEIVFYAAQVLDGTVLFEGSASRKISVVREVPLSVALEPHPSKVGDTETI"
BASE COUNT          189 a          411 c          370 g          205 t
ORIGIN      
        1 gaatcgtttc ggtatcgccc accttcgacg ggtggggttc cagcgccacg gacagcggga
       61 cctccctgac gaccgagatc ttccgggacg ccgatccctc gaagagcacc gtcccgtcga
      121 ggacctgggc ggcgtagaac actatctccg ccgtctttcc ctcccagggg gccgtgtcga
      181 aatgaagggc gtccccgtcc ccggcgaatt ccccgttgac ggaccactgg tatttcaggg
      241 ttcctccccc ctttctcggg aaggcggtgc ccgaaaaccg gaggggtgtt ccggccgcgg
      301 cctcggtgat atcaggttcg aggaggaccc ggagatctcc gtcggctcct ccgggaggca
      361 gaacctccag ggaaagggcg gatgccgaga cgaaccggtt cttcgtccgt gcctccaccc
      421 ggatctccac ggtccgcccc tcgtagcccg tgccgtcgaa ggatatggtc cgttccgccg
      481 tggaggcgat ccgcctcccg tccgcgaacc agatgtacca ttcgaaccct tcgcctccaa
      541 gaccgaactc ctccgtctcc gccgtgaggg gaacaatttc ccccgcccgg aaggaatcca
      601 cggggcaggt cagccgcagc cgcggacggg gcgtatcggc tgcggagagg acggtcaccg
      661 ggtgaacggc ccgtacccag tcggctccgg gaagatcggc gaggacatgc acttccagga
      721 ccttctttcc cggggacgag ggagtccacc ggatgcggga cgtcgtgtcc ccctcaaggg
      781 cctccccgtc gccgccggag gcgaacttcc agtcgtaccg ggccgaccgg agctccctgt
      841 ccaggacgac cgtaaaaaac tcttccatcc cctcgcccac ggtgtccgga ccagaaatcc
      901 gcacgccggg aggggtctgc ctgctgcgga gggattcgag atattccctg tattcgctga
      961 tcatccggcg ccgctccggg gtaccggagg gatagatcag ggcccccgcc ctcgtccgga
     1021 cggcgggctg cagggtcttt tcatcggccc cgttccggcc gacaatctcc tccacggcgg
     1081 cagccgcctc ccggagggcc tttttcgcct cctcgaggtc tttccggtcc tcttcgagga
     1141 cgatccggat cgattcggga gagcaggagg gcttc
//
LOCUS       DAOTHD010000837         1173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_144971_length_1173_cov_3.567084, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000837 DAOTHD010000000
VERSION     DAOTHD010000837.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144971_length_1173_cov_3.567084"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..>1173
                     /locus_tag="P5219_11825"
     CDS             33..>1173
                     /locus_tag="P5219_11825"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRX27500.1"
                     /translation="MDSRGAVLADTESDPSTMDNHLSRPEVKEALSVGEGTALRYSTT
                     TNMHYLYFARKISLDGGRTAVIRSAYPLEHLSAAFAETRNRFLLYLLVAVALIAVFGA
                     GLVRLFFRPLDRVVESAGKIAQGEEVRFPLMAEPELQRLSSALDGMSAQLRGTMEELR
                     SGREDLSRIVTALPVGVVLLDDGRRVRYMNGVAARLLAVRGDVPPGTPVERMLPSGEM
                     YGLILSALEGGEESRVFDLPELDGRCLGIASRKTATGVLLVVTDLTEERRIEQSRRDF
                     VADAGHEFQTPLTAIRAAAEYLLEAPEEETAGQRKKFLSSIIVQQERMSKLVDDLLLL
                     SRMESEPPLRDAEETDLALLLAGVADQCRTHPFASLIAVETELPPR"
BASE COUNT          205 a          372 c          382 g          214 t
ORIGIN      
        1 gggagggaat cctgagaggg agagtcaccc tcgtggattc ccggggtgcg gttcttgcag
       61 acacggaaag cgacccgtcg accatggaca atcacctttc ccgcccggag gtgaaggaag
      121 ccctgtcagt cggcgaggga acggcgttgc ggtacagcac caccacgaac atgcactacc
      181 tgtattttgc ccggaagatt tcccttgacg gggggcggac agcggtcatc cggtccgcct
      241 accccctgga gcatctttcg gcggccttcg ccgagacccg gaacaggttc ctcctctatc
      301 tcctcgttgc cgtggcgctg atcgcagtct tcggggccgg gctggtccgc ctctttttcc
      361 gcccccttga ccgggtcgtc gagtcggcgg gaaaaattgc ccagggagag gaagtccgct
      421 tccccctcat ggccgaaccg gaactgcagc gtctttccag cgcccttgac ggaatgtccg
      481 cccagctcag gggaaccatg gaggagctcc ggtccggccg ggaggatctc tcccggatcg
      541 tcacggccct tcccgtgggc gttgtcctgc tcgacgacgg cagaagggtc cgctatatga
      601 acggtgtcgc cgcccggctg cttgccgtac ggggagacgt gccccccgga acaccggtgg
      661 agcggatgct tccctccggc gagatgtacg gtctcattct ctccgccctg gaagggggag
      721 aggagagcag ggtgttcgac ctgcccgaac ttgacggacg gtgcctcgga atagcctcaa
      781 ggaagacggc gacgggggtg ctcctcgtgg tcaccgatct tacggaggaa cggcggatcg
      841 agcagtcccg cagggatttc gtcgccgatg cggggcacga gttccagacc cccctgaccg
      901 ccatccgggc ggcagcggag taccttcttg aagcgccgga ggaggagacg gcggggcagc
      961 ggaagaaatt cctttcatcc atcattgtcc agcaggaacg gatgagcaaa ctggtggacg
     1021 accttcttct gctttcgagg atggaatcgg aaccgcccct ccgggatgcg gaggaaactg
     1081 accttgccct tctcctcgct ggagtggcgg accagtgccg gacccacccc ttcgcctccc
     1141 tgatcgccgt cgaaacggaa ttgcctccgc ggg
//
LOCUS       DAOTHD010000838         1173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_144995_length_1173_cov_3.042934, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000838 DAOTHD010000000
VERSION     DAOTHD010000838.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144995_length_1173_cov_3.042934"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /locus_tag="P5219_11830"
     CDS             <1..1141
                     /locus_tag="P5219_11830"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HRX27501.1"
                     /translation="GVFGIASPNLFAQASPGPGVHLNRNIRTMSLRMAGELREKGAEV
                     VIALSRLSREENKHLAESVEGIHAILGGSSHEESPEPLFVRGPNGWETLLAESGAYGA
                     FIGKLLLDVRNGRISRQGTSWNLLRVTPEAGSHQEVERIARTFEERLNEALISTVGIF
                     ENYADGRALTVRSGESPLGNFITDALRWRFRTDIAMINGGGIRGDRIFPAGNVSWKTL
                     LEILPYNNPIHIVTLKGSQIRQILELSAGALKGGPDDHYDPNNRPHTGAFLQLSGLRV
                     EYSLRGIPSLVDTTGRLLRWGNRLRSVAVLSGEEWVPLEDGREYTVAVNSWTAGGGDR
                     LFVFAEGTAEKTDIRDIDTVAEYLMSRKDRRVRFEKDGRITITDS"
BASE COUNT          259 a          354 c          365 g          195 t
ORIGIN      
        1 gggcgtcttc ggcatcgcct ctcccaatct cttcgcccag gcgagcccgg ggcctggagt
       61 acacctcaac aggaatatcc ggaccatgtc cttgagaatg gccggcgaac tccgggaaaa
      121 aggagcggaa gtcgtcatcg ccctcagccg cctttcacgg gaggagaaca aacacctcgc
      181 ggagtcggtg gaggggatcc acgccatact cggaggctcc tcccacgagg agagtcccga
      241 gcctcttttc gtccggggcc cgaacggctg ggaaaccctt ctggcggaat cgggcgccta
      301 cggagccttc atcggaaaac tgctgctcga cgtaaggaac ggccggatca gcagacaggg
      361 gacatcatgg aatctcctcc gggtcactcc cgaagccgga agccatcagg aagtggaacg
      421 catcgcccgg actttcgagg aaaggctgaa cgaagccctt atctccacgg tagggatctt
      481 tgaaaactat gccgacggca gggctctcac ggtgagatcg ggggaaagcc ccctcggaaa
      541 cttcatcacc gacgccctgc ggtggcgctt caggacggac atcgccatga tcaacggcgg
      601 aggcatccgg ggcgacagga ttttccccgc gggaaacgtg tcctggaaga cactgctgga
      661 gatattgccc tacaacaacc ccatccatat cgtgaccctg aagggcagtc agatccggca
      721 aatcctcgaa ttgtcagccg gtgcgctgaa aggcggcccg gacgatcatt acgacccgaa
      781 caaccgcccc cacaccgggg cgttcctcca gctctccggc ttgcgggtgg aatactctct
      841 ccgggggatt ccctccctcg tggatacaac cggcaggctc ttacggtggg gaaaccggct
      901 ccggagcgtg gccgtcctca gcggtgaaga gtgggttccc ctggaagacg gcagggagta
      961 caccgtggcg gtgaattcct ggaccgccgg gggaggcgac aggctcttcg tctttgcgga
     1021 aggaacggcg gaaaaaacgg acatcaggga catcgacacc gtggcagaat acctcatgag
     1081 caggaaggac aggcgggtcc ggttcgaaaa ggacgggagg atcaccatca ccgattcctg
     1141 agagcaatac gccgttcgtt gtcatccggt tcg
//
LOCUS       DAOTHD010000839         1173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_145034_length_1173_cov_2.434705, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000839 DAOTHD010000000
VERSION     DAOTHD010000839.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145034_length_1173_cov_2.434705"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="P5219_11835"
     CDS             complement(<1..744)
                     /locus_tag="P5219_11835"
                     /inference="COORDINATES: protein motif:HMM:NF012912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HRX27502.1"
                     /translation="MPVSIVVGLQWGDEGKGKMVDHLAESASAVARFSGGSNAGHTVE
                     AGGGKFALHLLPSGLVRPEMKAMIGAACVMDPVTLLQEVASLEEAGVPVGSRLTISGK
                     TNLTHPAYRLLEKRREEAMGQGRIGTTGRGIGPTYEMKFRRCGLRLEDALEKENFVSM
                     TGSVTDEFAVSLKLTGEEKAILRKEAEVFTEASFKVATWCGDVSRGIGELLARGERVI
                     AEGAQGSLLDPDHGSYPFVTCGQCVAGGAC"
     gene            complement(746..>1173)
                     /locus_tag="P5219_11840"
     CDS             complement(746..>1173)
                     /locus_tag="P5219_11840"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein 8"
                     /protein_id="HRX27503.1"
                     /translation="RPGCTQFINIFTTNRDFYIADLPGYGFAKAPERLRSQWIDWIGW
                     YLRERKPLRGTVLLVDARHPDLENDRSMAEYLMEGGKPYIVALTKSDKLKRGKLAASV
                     RQAGEMGPVIPVSSMDGSGMNELCRWLADTTAAGMPVGS"
BASE COUNT          184 a          404 c          326 g          259 t
ORIGIN      
        1 gcaggccccg ccggcaacgc actgtccgca tgtcacgaag ggatatgagc cgtggtccgg
       61 atccagaagg gaaccctgtg cgccttcggc tatgaccctt tcgcccctcg caaggagttc
      121 tccaataccg cgggagacgt cgccgcacca ggtcgccacc ttgaaggaag cctcggtgaa
      181 gacctccgcc tctttcctga ggatcgcctt ctcctccccg gtcagcttca gggaaacggc
      241 gaattcgtcg gttaccgatc cggtcatcga tacgaagttc tccttttcca gggcgtcctc
      301 cagccggaga ccgcaccggc ggaacttcat ctcgtaggtg ggtcctatgc cccttcctgt
      361 ggtgcctatc ctcccctgcc ccatggcctc ttcccggcgc ttctccagaa gcctgtaggc
      421 agggtgggtc aggttcgtct tcccggatat cgtcaggcgg ctgcccacgg ggacaccggc
      481 ttcctccagg gacgcgactt cctgaagcag cgtcaccggg tccatcacgc aggccgcgcc
      541 tatcattgcc ttcatttcgg gtctcaccag cccggagggg agcaggtgca gggcgaattt
      601 tccaccgccg gcctccacgg tgtggcctgc gtttgatccc ccgctgaatc tggccaccgc
      661 cgaagcgctt tcggcgaggt ggtcaaccat tttgcccttg ccctcgtctc cccactggag
      721 tcccactacg atgcttactg gcattctagc tccccactgg catcccggca gccgtagtgt
      781 cggccagcca tctgcatagt tcgttcattc cgctgccgtc catggaggac acgggaatga
      841 ccggccccat ctcccccgcc tgccttacag aggcggcgag ctttccgcgc ttcagtttgt
      901 cgctcttggt aagggcgact atgtagggct ttcccccctc catgaggtac tccgccattg
      961 acctgtcgtt ctccaggtcg gggtgtctgg cgtccaccag gagcacggtc cccctgaggg
     1021 gtttccgctc cctcaggtac caccctatcc agtcgatcca ctggctcctg agcctttccg
     1081 gagccttggc gaatccgtag cccggaaggt cggctatgta aaaatccctg tttgtcgtga
     1141 atatgttgat gaactgggtg caacccggcc tgg
//
LOCUS       DAOTHD010000840         1173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_145038_length_1173_cov_2.313059, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000840 DAOTHD010000000
VERSION     DAOTHD010000840.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145038_length_1173_cov_2.313059"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="P5219_11845"
     CDS             <1..598
                     /locus_tag="P5219_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27504.1"
                     /translation="VTPNSLGESQDLEYLYWKIWDALMTELPIKEDFEEKIIRTNIDG
                     FTETYAMSDVMPRQAKAGGRIDVNLFKGIQDTWDERQGLNGVDVKIPVREALVKSASA
                     AGTDEQAVVQYFRNPASDKRIVIFGHSHESRMIPSETHDGKKALYVNSGTWIDKNATP
                     TMTFVTVIPKGGERHVGLYQYSHDGSIGTLDSMEVPGF"
     gene            complement(661..>1173)
                     /locus_tag="P5219_11850"
     CDS             complement(661..>1173)
                     /locus_tag="P5219_11850"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HRX27505.1"
                     /translation="ASIGLAALNALVDEGEAGESSNDDLVEMLGITPKDRVGMVGDIM
                     PLLRMIRDHAGHCVVFDEGKNEEKGITSTDLEGEELPGCSVVLLSATSLLNGTFDDVL
                     SMASGAREICVIGPSAPLLPDIFRDRGVTMLSGRRFTDVDRLLRIVSEAGGTRCFGPV
                     SVKVNIRLKE"
BASE COUNT          250 a          382 c          339 g          202 t
ORIGIN      
        1 ggtcaccccg aattccctcg gcgaaagcca ggacctcgaa tacctctact ggaaaatatg
       61 ggatgccctt atgaccgaac tccccataaa agaagacttc gaagaaaaga tcatcaggac
      121 caacatcgac ggcttcaccg aaacctacgc catgagcgac gtcatgcccc gccaggccaa
      181 agcgggcggc cgtatcgacg tgaacctctt caaagggatc caggacacct gggacgaacg
      241 gcagggcctc aacggagtcg acgtcaaaat tcccgttcgg gaggctctcg tcaagtcggc
      301 ctccgcggcg gggaccgatg agcaggccgt ggtgcagtac tttcggaacc ccgcgtccga
      361 caagcggatc gtgatcttcg gccacagcca cgaatccagg atgatcccct cggagaccca
      421 cgacgggaaa aaagctctgt acgtgaactc cggcacctgg atcgacaaga acgccacgcc
      481 caccatgacc ttcgtcacgg tcattccgaa gggcggggag cggcacgtgg ggctgtacca
      541 gtattcccac gacggaagta tcgggacgct ggacagcatg gaggttcccg gcttttaacg
      601 ggccgttcct gcagcaggaa acaagagagc ccggcggttc cttcgccggg ctctcttcgt
      661 ttattctttc agccggatat tcaccttcac gctcacgggg ccgaagcacc tcgtcccgcc
      721 agcctcgctg acgatccgga gcagacggtc cacgtcggtg aatcgccgtc cggacagcat
      781 cgtgaccccc cggtcccgga aaatgtccgg cagcagggga gcggacgggc cgatgacgca
      841 gatctccctc gcccccgagg ccatggaaag aacgtcgtca aaggtgccgt taagcaggct
      901 cgtcgccgag agaagcacca cgctgcaccc gggaagttcc tccccctcca ggtctgtgga
      961 ggtgatgcct ttttcttcat ttttcccctc gtcgaagacc acgcagtgcc cggcatggtc
     1021 ccggatcatc cggaggaggg gcatgatgtc cccgaccatt cccaccctgt ccttcggggt
     1081 tatgccgagc atctccacca ggtcgtcgtt ggaggactcc ccggcttcgc cctcgtcgac
     1141 aagggcgttc agggccgcga gaccgatgga agc
//
LOCUS       DAOTHD010000841         1172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_145141_length_1172_cov_3.720680, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000841 DAOTHD010000000
VERSION     DAOTHD010000841.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145141_length_1172_cov_3.720680"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="P5219_11855"
     CDS             complement(<1..822)
                     /locus_tag="P5219_11855"
                     /inference="COORDINATES: protein motif:HMM:NF017078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HRX27506.1"
                     /translation="MPIRLRTLLIVVASTLLFLGGMSALSGMLVLDSYRDLEFQSLFD
                     NCARAENILSREADALLRVNRDWAFWDDTYAYMDDMSGLYEAVNLQEETLETLHLAAM
                     VFLSPDGTVVSSGPVLTDGAAAFMDRIRHQGTSLAGKAGGDGFSGFFLNQDRLFMVSG
                     APILRSDGSGPPKGVLLFVRKIGEQEAASLTESLLLPLKIHPAGDSLPENLREMLDSV
                     TSAEKGFLSRPVSPEVITGFSVLRDIDGLPAALLEISSDPGAWSHPRPGIFLRVSP"
     gene            982..>1172
                     /locus_tag="P5219_11860"
     CDS             982..>1172
                     /locus_tag="P5219_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27507.1"
                     /translation="MYFFEMLLSALSLSMDALAASLGIGACLGAATGGAAALRVAGAC
                     GGFQFAMPLAGWFLGSRFLV"
BASE COUNT          244 a          328 c          376 g          224 t
ORIGIN      
        1 cggggatacc cgaagaaata tccctggccg tgggtggctc catgctccag ggtccgagga
       61 gatttcaagc agggctgccg gtaacccgtc aatgtcccgg aggacggaaa aaccggtgat
      121 cacctccgga gagactggcc gggagaggaa acccttttcc gccgacgtca ccgagtccag
      181 catttccctc agattttccg gaagggaatc gccggcaggg tggattttca gcggaagcag
      241 aagagattcc gtcagggagg ccgcctcttg ctccccgatt ttcctcacga agaggagaac
      301 ccctttcggt ggtccggagc cgtcgctcct gaggatcggc gcaccggaca ccatgaagag
      361 cctgtcctgg ttgagaaaaa aaccggagaa tccgtcaccc ccggccttgc cggccaggga
      421 ggttccctgg tgcctgatcc ggtccataaa ggccgcggca ccgtcggtga ggacaggtcc
      481 cgaagagacc accgtcccgt ccggggagag gaagaccatg gcggcgaggt gcagggtttc
      541 cagggtctcc tcctggaggt tcacagcctc gtacagtccg ctcatgtcgt ccatgtaggc
      601 ataggtgtcg tcccagaagg cccagtcgcg gttgacccta agaagggcat ccgcttcccg
      661 ggagagaatg ttctccgccc tggcgcaatt atcgaagagg gattggaatt caagatccct
      721 gtagctgtcc agaacaagca ttccggagag ggctgacatt ccccccagga agaggagcgt
      781 ggaggcaacg acgataagga gggttctcag gcgtatcggc atcgggacca tcgcctttcg
      841 ggatgtgtgg atcatgattt ggaatgaaaa aaacaggaaa attataacag aaaagaaaga
      901 gttgatggaa ggcgttcccg gggaaaagtg gtatgcttac gggaagccaa agaacgtacc
      961 ccatgaaagg tcggttttcc catgtatttt ttcgagatgc ttctctccgc cctatccctc
     1021 tccatggatg ccctggcggc gtccctcggg atcggggcgt gcctcggcgc cgccacgggg
     1081 ggcgcggcgg cccttcgggt ggccggggcc tgcggagggt ttcagttcgc catgcccctg
     1141 gccgggtggt tcctgggcag ccgcttcctg gt
//
LOCUS       DAOTHD010000842         1172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_145174_length_1172_cov_2.850492, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000842 DAOTHD010000000
VERSION     DAOTHD010000842.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145174_length_1172_cov_2.850492"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="P5219_11865"
     CDS             complement(<1..1157)
                     /locus_tag="P5219_11865"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRX27508.1"
                     /translation="MAHVSGETRLAQPFEEYSAGLTEEQTLRDAKRCMECGCADVFEC
                     RLRKYATTHEVQPEKLAGEHVAKFEDANQYYVRNLDKCILCSKCVRACDEIAGFHAID
                     FAKRGFESVLTPQFYNDMEHSDCTFCGLCTQVCPVGALMEKRAERWPHLEEPEIIKTT
                     CQMCSVGCELDLNLDRRRSRIVRVTTDLDNPTAPTAGSCCFMGRYGFRDVRDSGNFDP
                     AVNGDSVSLDEAVAAFDSLAAKNGAVFVAGADLTLQEARALRDYAALRTPEAVISAAE
                     GAEFLPFLTEAGKRKDIVRASYASFNEGDAFLLLGANTDEDQPVLTSWLRRAMRHRKA
                     AVICLGGTPGILDRGDAVILAPDAGKETQAIVALTAAVRAAREKTSADFSAL"
BASE COUNT          191 a          370 c          374 g          237 t
ORIGIN      
        1 agggctgaaa aatccgcaga agtcttctcc ctcgcggccc ggacggcagc ggtgagggcc
       61 acgattgctt gtgtctcttt gcccgcgtca ggggcaagga tcacggcgtc tcccctgtca
      121 agtatgcccg gcgtgccgcc gaggcagatc acggcggcct tccggtgccg catggcccgc
      181 ctcagccacg atgtcagcac cggctggtct tcgtcggtgt tcgcccccag aagcaggaag
      241 gcgtcccctt cgttgaagga ggcgtaggaa gcccggacga tatcctttct ctttcccgcc
      301 tctgtaagga agggcaggaa ttccgctcct tccgccgcgg aaatgaccgc ttccggtgtg
      361 cggagcgccg catagtcgcg gagagctctt gcctcctgca gggtgagatc cgctcccgca
      421 acgaacaccg caccgttctt tgccgcaagg ctgtcgaagg cggccacggc ttcgtcaagg
      481 gacacggagt cgccgttcac cgcagggtcg aagttcccgg agtccctcac gtcccggaag
      541 ccgtaccgtc ccatgaagca gcacgatccg gcggtggggg ctgtcggatt gtccaggtcg
      601 gtggtgaccc ggacgatccg ggagcgcctg cggtcgaggt tgagatcgag ttcgcagccc
      661 acggagcaca tctggcaggt ggtcttgatg atttcaggtt cctccaggtg gggccaccgc
      721 tcagcccgtt tttccatgag ggcccccacg gggcagacct gggtgcagag tccgcagaac
      781 gtgcagtcgg agtgttccat gtcgttgtag aactggggcg tcagcaccga ttcgaagccc
      841 cgcttcgcga aatcgatggc gtggaagccg gctatttcat cgcaggcccg gacacacttg
      901 gagcagagaa tgcacttgtc caggttccgg acatagtact ggttcgcatc ctcgaatttg
      961 gcaacgtgtt cgccggcaag cttctccggc tgtacctcgt gggtggtggc gtatttccgg
     1021 agcctgcact cgaagacatc ggcgcagccg cactccatgc atctttttgc gtcccggagg
     1081 gtctgttcct cggtcagccc tgcgctgtac tcctcgaagg gctgtgcgag ccgcgtctct
     1141 ccgctcacgt gtgccacgtg ctcctggacg gc
//
LOCUS       DAOTHD010000843         1168 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_145954_length_1168_cov_3.205750, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000843 DAOTHD010000000
VERSION     DAOTHD010000843.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145954_length_1168_cov_3.205750"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..730)
                     /locus_tag="P5219_11870"
     CDS             complement(110..730)
                     /locus_tag="P5219_11870"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HRX27509.1"
                     /translation="MCLLFLLFGVSGGTSLFLGLLLANISAGYMLFFFRDPDRATPSD
                     PGLVVAAASGKLAKIAQVYEGEYLKTDTVRISIFLSLLDVHVNRFPIGGSSTFLGYFP
                     GKRLFTFDEKSSDVNQHNTILVKGEGTSCLITQIVGPVCRRVVYWLRPDKTVSVRRGQ
                     RFGMMKFGSRLDMYFPKGDIEILAQIGEQVTAGQTVIARIVNRENR"
BASE COUNT          260 a          308 c          369 g          231 t
ORIGIN      
        1 aggatgctgg cgaagccgaa gaagagcgcc gaaagggtca ccagggtcgg gacgatacgg
       61 cgccagttgg ccagggtccg gagcttctgg aggtggtcct tcgtcatgat taccggttct
      121 ccctgttcac gatccgggcg atgacggtct ggcctgcggt cacctgttcg cctatttggg
      181 cgagaatctc gatgtcaccc ttggggaagt acatatcgag ccgggagccg aacttcatca
      241 tgccgaatct ctggcctctc ctcacggaca ccgtcttgtc gggacggagc cagtagacaa
      301 ccctgcggca cacgggaccg acgatctgcg tgatgaggca tgacgttccc tctcccttga
      361 cgaggatggt attgtgctgg ttcacgtcgg aggatttctc gtcgaaggtg aagagtctct
      421 ttccggggaa gtagccgagg aaggtggagg agcccccgat ggggaaacgg ttcacgtgga
      481 cgtcgagaag actgaggaag atgctgatgc gcacggtgtc tgtcttcagg tattcacctt
      541 cgtagacttg ggcgatcttg gcaagcttgc cgctggccgc cgcgacgacc agccccgggt
      601 cggacggtgt ggccctgtcg ggatcccgga agaaaaagag catgtagccg gccgaaatat
      661 tggccagcag cagtcccagg aacagggacg taccgccgga aacgccgaag agcaggaaaa
      721 gcaggcacat gaccgctccg aacgctgccg aagcaaaaag aaacggtttt acctctggac
      781 ggaatttcat aaaataaagc ccccttgttt tctgtttgtc gaataattat accgtacatg
      841 gctggagagg tcatctgaaa tccggacagt tccccgaatg ttgcgtcagt acatttacgg
      901 aatttttctc ttttgcggga aagcgcggca ggagcccgca ggttgaccga cgatttccaa
      961 tggggtataa gggggacgcg gcgaaagccg caggaggacc cgaatggaga accgcgaatt
     1021 ttttcccgga cgggaatccg gcgagggaag gacgggcact ccccttttcc gggcatcgtc
     1081 aatcacgaaa atctatcacc agggaaccca gaccgaggtc cgcgcccttg acggggtgga
     1141 tctcgaactc ttcgacggcg agctggcg
//
LOCUS       DAOTHD010000844         1168 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_145990_length_1168_cov_2.637916, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000844 DAOTHD010000000
VERSION     DAOTHD010000844.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145990_length_1168_cov_2.637916"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..>1168
                     /gene="cheB"
                     /locus_tag="P5219_11875"
     CDS             139..>1168
                     /gene="cheB"
                     /locus_tag="P5219_11875"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HRX27510.1"
                     /translation="MIRVLMVDDSPLMCRILGQMLESDPGIRVWGTAGSGEEALALLQ
                     KGRPDVVTMDIHMGGMDGFETTRRIMESERPLPVVIVSSCWDPLEVEKTFDAMAAGAV
                     AILPKPANIAEEGGEYEQEFLRTIRAASEARVGRLRKKFPDPGAPLPAAGKGRVKIVA
                     AGASTGGPQALAAFLSGLPAGFPRPVLVVQHMAPGFTRGLADWLGKITPLRVKIGTQG
                     EPLSGGTVYIAPEGTHMVLNRSGDSLRFSDGPPLHMVRPSVSMLFRSVAEVYGRDSAA
                     LLFSGMGADGAEELKQLRNLGAATFAQDRESSVVWGMPGEAVRLEGAEHVGPPSVLAA
                     ILSGMAAGS"
BASE COUNT          211 a          336 c          403 g          218 t
ORIGIN      
        1 aatcggcgga ggacagagag cggggcgtgg agtcaggcgc cgacgcctac atcgtaaaaa
       61 gcggtttcga ccagggaacg ctcctggacg tcatccggcg gctgctctga gctggccgcc
      121 gtgaaaggtg ggggttcttt gatcagggtc cttatggtgg atgattcgcc cctcatgtgc
      181 agaatcctcg gccagatgct cgaatccgat cccggaatac gggtctgggg aacggcggga
      241 agcggggaag aggccctggc ccttctgcaa aagggcaggc ccgatgtggt cactatggat
      301 atccacatgg gaggcatgga cggcttcgag accacgaggc ggatcatgga atcggagcgc
      361 ccccttcccg tggtgatcgt cagctcatgc tgggatcccc tcgaagtgga gaagaccttc
      421 gatgccatgg cggcgggggc ggtggcaatc ctccccaagc cggcgaatat tgccgaagag
      481 ggcggagaat atgaacagga gtttctgcgg acaatccgtg ccgcatcaga ggcccgtgtc
      541 ggccgcctca ggaagaaatt cccggatccc ggcgcaccgc tgcccgccgc cgggaaaggc
      601 agggtaaaaa tcgttgccgc gggcgcttcc acgggagggc cccaggctct tgccgccttt
      661 ctctccggcc ttcctgcggg atttccccgg cccgtcctcg tggtgcagca tatggctcct
      721 gggtttaccc ggggactggc ggactggctc ggaaaaatca cccctttgag ggtgaaaatc
      781 ggtacccagg gggagcccct gtcgggagga accgtctaca tcgctcctga gggaacgcac
      841 atggtgttga acaggagcgg ggattctctc cgcttttctg acggacctcc cctgcacatg
      901 gtccgtcctt ccgtgtcgat gcttttccgt tcagtggcgg aggtttacgg cagagactcc
      961 gccgcgctgc tcttctcagg aatgggggcc gacggggcgg aagaactgaa gcaactgagg
     1021 aatctcgggg cggccacttt tgcccaggac agggagtcct cggtggtctg gggaatgccg
     1081 ggggaggccg tccgccttga aggtgcggag cacgtggggc ctccatcggt tcttgccgcc
     1141 atcctgtcgg gcatggcggc cggaagca
//
LOCUS       DAOTHD010000845         1166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_146338_length_1166_cov_2.922592, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000845 DAOTHD010000000
VERSION     DAOTHD010000845.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146338_length_1166_cov_2.922592"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1166)
                     /locus_tag="P5219_11880"
     CDS             complement(<1..>1166)
                     /locus_tag="P5219_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27511.1"
                     /translation="IMKFLVIKIAGKVLSKLVPVIGWALLAFEVYDMAGARDRLEEEM
                     RKSFLEEYKAEISAETLWWKGENGSGTSMREEVDRNIGSLLNNWERICRSEADSMIQS
                     AHILSFSEEVREYVSKQIRDGSEFEPVLQKMSILWDVFGQLLAQGQVQVFESMLVAAP
                     DRNDLRLLAKSLGQKLPELYKKYGRDFLQAVSKIGVQNYLSSEEWSRGEIDWHILNEN
                     LLSLPELGDNREAAAGLLTMVLEGAPVRGLSPDALATVAGQKELFRKIWQILRPEMNK
                     TILLLGNRRAAAHIGESLELYPAAASLFLKSYGPEFWSAWSREEIVDLLQITDFRAVN
                     SGKTHEVVLVPPEDRAELINIFRKAGKPGIALWDIYATEGTGAVGKNLARESVE"
BASE COUNT          231 a          340 c          298 g          297 t
ORIGIN      
        1 ttccacactc tctctggcga ggtttttccc gaccgccccg gtgccttccg ttgcgtatat
       61 gtcccatagg gctattccag gttttcccgc cttgcggaaa atgttgatga gctcggccct
      121 gtcttcggga ggaacgagga caacctcatg ggtttttccg gaattgacgg cccggaaatc
      181 ggtaatttgg agaagatcga cgatttcctc ccgggaccat gcgctccaga actccggtcc
      241 gtagctcttc aggaataagg atgcggcagc cgggtagagt tccaggcttt cgccgatgtg
      301 ggcggcggct cttctgttcc cgagaagaag aattgtcttg ttcatctcag ggcgaagaat
      361 ctgccagatt ttcctgaaaa gctccttctg ccccgccact gtagccaaag cgtcgggtga
      421 cagccccctg acgggagcgc cttcaagcac catggtcagc agtccggcgg ccgcttccct
      481 gttgtctcca agttccggaa gggaaaggag gttttcgttg agaatatgcc agtcgatctc
      541 tcccctgctc cattcctcag aggataaata attctgcact cctattttgc tgacggcctg
      601 caaaaagtca cggccgtatt ttttataaag ttccggcagt ttctgcccga ggcttttcgc
      661 gagcagcctc aggtcgtttc tatcaggggc tgccacgagc atggattcaa agacctggac
      721 ctgcccctgg gccagcagtt ggccaaacac gtcccacagg atactcatct tttggagcac
      781 cggctcgaat tcggatccgt ctctgatttg ttttgaaaca tactccctga cctcttcaga
      841 gaaggaaaga atgtgggcgc tctgtatcat ggagtccgct tcagacctgc agatccgctc
      901 ccagttgttc agcagggatc cgatgttccg gtccacttcc tcccgcattg aagttccgga
      961 gccgttttcc cctttccacc acagagtctc ggcactgatc tccgccttgt attcttcgag
     1021 gaaggatttt ctcatctcct cttcaaggcg gtcccgggcc cctgccatgt cgtacacttc
     1081 gaaggcgagc aatgcccatc cgatgaccgg cacgagcttc gagagaacct ttccggcgat
     1141 cttgataacg aggaatttca tgattc
//
LOCUS       DAOTHD010000846         1166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_146453_length_1166_cov_1.542754, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000846 DAOTHD010000000
VERSION     DAOTHD010000846.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146453_length_1166_cov_1.542754"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="P5219_11885"
     CDS             complement(<1..228)
                     /locus_tag="P5219_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27512.1"
                     /translation="MKKKIRRCIPGALPVLAVLLLAACWAGGTAAYGAVPALNIPRMI
                     QECPHFSDHPGSNGVIWLKDSEYSLGADGSMT"
     gene            complement(257..>1166)
                     /locus_tag="P5219_11890"
     CDS             complement(257..>1166)
                     /locus_tag="P5219_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HRX27513.1"
                     /translation="LLRLFGSERIQGLMEKLGMEEGESIEHPLLTKAIASAQKKVEEM
                     HFDIRKQLLAYDNVMNRQREAVYAERQQILSDDGIVDRIWEVIDGVVEDILEKYFPEE
                     GESDPGRAQARLKAIFGPGLEKHLDGVDNPRDMLGRKDGLKEEIRNRFLRKVETLGEE
                     EGGALLRFLILHTLDNGWKDHLLAMDELRRGIGLRAIGQKDPLLEYQFESYNLFQGMM
                     ERVRESVAELAFRVRVVSDTGRGSPSELRESREFVLPFGGNADHPAPVGEGPRQPVKK
                     GPKVGRNDPCPCGSGKKYKHCCGAGK"
BASE COUNT          191 a          394 c          331 g          250 t
ORIGIN      
        1 ggtcatggag ccgtcggcac cgagagaata ctccgagtcc ttcagccaga tcacgccgtt
       61 gcttccgggg tggtccgaga agtgggggca ctcctggatc atcctgggga tgttcagcgc
      121 aggaacggca ccataggcag cggtccctcc cgcccagcag gccgccagca gaaggacggc
      181 gagaaccgga agggctcccg ggatacatct tctgatcttc tttttcatgc ttccatcccc
      241 tttcctgcgc ccgggcctac ttgccggcgc cgcagcaatg cttgtatttt tttccgctgc
      301 cgcaggggca gggatcattc cgtcccacct tgggtccttt tttcacaggc tgccggggcc
      361 cctcacccac aggcgcggga tgatcggcat tcccgccgaa gggaaggaca aactccctgc
      421 tctccctcag ttccgaagga ctgccccggc ccgtgtcgga caccacccgg accctgaagg
      481 cgagttccgc caccgattcc ctgaccctct ccatcattcc ctggaacagg ttgtaggatt
      541 cgaactgata ctccaggaga ggatccttct ggccgatggc cctgagaccg attccccggc
      601 gcagctcgtc catggcaagc aggtggtcct tccagccgtt gtccagggtg tggagaatga
      661 ggaaccggag cagcgcccct ccctcttcct cgccgagggt ttccaccttg cggaggaagc
      721 ggttccggat ttcctccttc aggccgtcct tccggccgag catgtccctg gggttgtcca
      781 ctccgtcgag atgtttctcg agtccgggcc cgaaaatggc cttcagcctg gcctgggccc
      841 ttcccgggtc ggactcccct tcttcgggga agtacttctc caggatatcc tccaccacgc
      901 cgtcgatcac ttcccagatg cggtccacga tgccgtcgtc cgagagaatc tgctgcctct
      961 cggcatagac ggcctcccgc tgccggttca tcacgttgtc gtaggcgagg agctgcttcc
     1021 gtatgtcgaa gtgcatctcc tccacctttt tctgggcgga ggcgatggcc ttggtgagaa
     1081 gggggtgttc gatggactcc ccctcctcca ttcccagctt ctccatcagc ccctggatcc
     1141 tctccgaacc gaagaggcga aggaga
//
LOCUS       DAOTHD010000847         1165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_146513_length_1165_cov_3.210811, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000847 DAOTHD010000000
VERSION     DAOTHD010000847.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146513_length_1165_cov_3.210811"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="P5219_11895"
     CDS             complement(<1..570)
                     /locus_tag="P5219_11895"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRX27514.1"
                     /translation="MAIEELNKNMLEAAKLGDIEGVKKVLEQGADVNTRSKDGRTALI
                     EAARWGRLTSTLLELLATHKADVNAVNEYGWTAMMIALNKGHLTFPLLKTFKKNGANI
                     ERDYEWLKKEGRLSPEVDAMFLEAMKEPEVKKDIEKLNWQLFKAAEKGKIQDVKNAIE
                     QGADVNARNNKGHTVLEACFFEWPFSTDVA"
     gene            complement(589..885)
                     /locus_tag="P5219_11900"
     CDS             complement(589..885)
                     /locus_tag="P5219_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27515.1"
                     /translation="MSYETCKGKSLGNYGIPAAIPALSLSPENIQAMVDILLDAMDSE
                     PFLVVEFIRKWVGKSLETCVSAGVKGTEGLVAKLVSYLKQRRNKKDETIPFMEF"
     gene            complement(866..>1165)
                     /gene="alaE"
                     /locus_tag="P5219_11905"
     CDS             complement(866..>1165)
                     /gene="alaE"
                     /locus_tag="P5219_11905"
                     /inference="COORDINATES: protein motif:HMM:NF018333.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034639 - L-amino acid efflux
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HRX27516.1"
                     /translation="AIPVNLITGRPYGLFRDRLFLALGIERKNPWKMILGDTLAFVIF
                     QVPLYVIVLLCAGATWKQIAVSSVFMSAIFSLAGRPYGIFLDFCRRVAGNVVRNL"
BASE COUNT          250 a          335 c          257 g          323 t
ORIGIN      
        1 tgccacgtct gtcgagaaag gccattcaaa aaaacatgct tcaaggacgg tgtgcccctt
       61 gttgttccgg gcgttcacgt ccgccccctg ctctatggcg ttcttcacgt cctgtatttt
      121 ccctttttct gccgctttaa ataactgcca gttcagtttt tcgatatcct tttttacttc
      181 aggctctttc atggcttcaa ggaacatggc gtcaacttcc ggggacaacc tgccttcttt
      241 cttgagccac tcgtaatctc tttcgatatt cgccccgttt ttcttgaatg tcttcagcag
      301 agggaaagtg agatgacctt tattcagcgc aatcatcatc gccgtccagc cgtactcatt
      361 cacagcattc acgtccgctt tatgggtggc aagcaattcc agcagtgtgg atgtgagacg
      421 cccccaccgt gccgcctcta tcaaagccgt cctgccgtcc ttgcttcggg tgtttacgtc
      481 cgcaccctgt tccagaactt ttttcacgcc ctctatgtcg cccagttttg ccgcttcaag
      541 catatttttg ttcagttctt caatggccat ctttatcctc tcccctggtt aaaactccat
      601 aaagggaatg gtttcgtcct ttttgtttct cctctgtttc aggtacgata ccagtttcgc
      661 caccaaccct tccgtcccct tcactccagc gctgacgcag gtttcgagac tctttcctac
      721 ccatttccgt ataaactcta ccaccagaaa cggttcggaa tccatggcgt cgaggagaat
      781 atccaccatc gcctggatat tttcaggcga cagggacagt gcggggattg ctgccggaat
      841 cccgtaattt cccagggatt ttcccttaca ggtttcgtac gacattcccg gcgaccctcc
      901 tgcaaaaatc aagaaagatt ccatacgggc gacctgccag agagaagatt gccgacatga
      961 aaaccgacga aacagcgatc tgtttccatg tcgctcctgc acaaagaagc acaatgacgt
     1021 agaggggtac ctgaaaaatg acgaaggcaa gcgtatcgcc caggatcatc ttccaggggt
     1081 ttttcctttc aattccgagg gcaagaaaaa gccggtcgcg gaacaggccg tagggcctcc
     1141 cggtgatgag gttgactggt atggc
//
LOCUS       DAOTHD010000848         1165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_146545_length_1165_cov_2.654955, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000848 DAOTHD010000000
VERSION     DAOTHD010000848.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146545_length_1165_cov_2.654955"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="P5219_11910"
     CDS             complement(<1..334)
                     /locus_tag="P5219_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HRX27517.1"
                     /translation="MRTEDIIRLIKESKKKTPCIAYLSGDLEGMDAGGAVFVGGKDFG
                     ILSGDRGDVEKILSENAPRIRSVHVETRARNSAVPLADLTKYEARIEPGAVIRDMVDI
                     GKGAVVMMG"
     gene            complement(313..966)
                     /locus_tag="P5219_11915"
     CDS             complement(313..966)
                     /locus_tag="P5219_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HRX27518.1"
                     /translation="MRYGLVGVSGRMGAEIRKAFASHELCYTLDIGGETCEGTPEVMV
                     DFSLPSALGATLDAVRRFSCALVLGTTGITGEQMDEVRYAARTSAVVQSFNFAAGVTL
                     FKMILREFGPLFADWDAEMSEIHHNRKKDAPSGTAILLREALGRDCPIHSLRMGGVPG
                     DHTVSFANEGEVFTLTHRAISRSVFALGALRAAEFAAGAKPGFYSFEEVLTCGRKTS"
     gene            complement(963..>1165)
                     /locus_tag="P5219_11920"
     CDS             complement(963..>1165)
                     /locus_tag="P5219_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRX27519.1"
                     /translation="EKARELHHRMFPLMKNLFMESNPIPVKYAVNRLGLCRNELRLPL
                     VPATEKCMAVIDESLKECGVSL"
BASE COUNT          211 a          391 c          347 g          216 t
ORIGIN      
        1 cgcccatcat caccacggca cccttgccga tgtccaccat gtcccgtatc accgcgccgg
       61 gctcgatccg ggcctcgtat ttcgtcaggt ccgccagggg aacggcggaa ttccgggccc
      121 tggtctccac gtggaccgac ctgatcctgg gcgcgttctc cgacaggatt ttttcgacgt
      181 ccccccggtc accggacagg atgccgaagt ccttcccgcc gacaaagaca gccccgccgg
      241 cgtccatacc ctcgaggtcc ccggacaggt aggcgatgca gggcgttttc ttcttcgatt
      301 ccttgatgag ccttatgatg tcttccgtcc gcatgtgagg acctcctcga aagaatagaa
      361 tcccggcttc gccccggcgg cgaattccgc cgcccgaagg gcccccaggg caaacacgct
      421 ccgggagatg gccctgtggg tcagggtgaa cacttctccc tcattggcga aggacaccgt
      481 gtggtccccc ggaacgccgc ccatccggag ggaatggatg gggcagtccc gcccgagggc
      541 ctcccgaagg aggatggccg tccctgaagg agcgtccttt ttcctgttgt ggtggatctc
      601 gctcatctcc gcatcccagt cggcgaaaag agggccgaac tcccggagga tcatcttgaa
      661 cagggtcacc ccggccgcga agttgaagct ctggaccacc gccgacgttc tggccgcata
      721 ccgtacctcg tccatctgtt cccccgtgat tccggtggtg cccagcacga gggcgcagga
      781 aaagcgccgc accgcgtcca gggtggcgcc gagggcggag ggcagggaaa agtccaccat
      841 aacctcaggc gtgccttcgc aggtctcccc gccgatatca agggtgtagc agagctcatg
      901 ggaggcgaag gccttccgga tctccgcccc catgcgcccc gacactccca cgaggccgta
      961 tctcataggc tcaccccgca ctccttcaga gattcgtcga tgacggccat gcatttctcc
     1021 gtggcaggaa cgaggggaag ccggagttcg ttcctgcaga ggccgaggcg gttcaccgca
     1081 tacttcacgg gaatgggatt cgactccatg aacaggttct tcatgagggg aaacatcctg
     1141 tgatggagtt cccgggcctt ctcca
//
LOCUS       DAOTHD010000849         1164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_146775_length_1164_cov_2.280433, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000849 DAOTHD010000000
VERSION     DAOTHD010000849.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146775_length_1164_cov_2.280433"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      111..220
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            276..1010
                     /locus_tag="P5219_11925"
     CDS             276..1010
                     /locus_tag="P5219_11925"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX27520.1"
                     /translation="MLIIHAFSKSFNGIPVFSDISLSAEQGSFTVLLGPSGCGKSTLF
                     DLLMGVLPPDGGRISLNGRERPDLRGAAAYMAQKDLLLPWKTLRENALLPVTVRRRPT
                     EEDLRRVEDLFSLLGLSGREDFFPGQVSGGMAQRCALARTILFDAPVALLDEPLSALD
                     AITRQTLRELLLLLQSRFGKTILMVTHDVEEALLLADTILVLSPPPMRILEVFRPEGT
                     KNSREESPSFPGMKRRILKLLREGSR"
BASE COUNT          215 a          362 c          334 g          253 t
ORIGIN      
        1 caccggcggc ccaagcgagc accggtgacg ctccccgctg cctgcggatt taggatttgg
       61 gtggtgcagg ggaaattgac aaatccaccg ggagggagta tcgtacccgt gacagcaact
      121 gggggagtcg gcgtaaaccg gctgagaggg agcttttcgg ctccgacccc tggaacctga
      181 tccgggtcat gccggcgaag ggaagtgcgt gtgcagcaaa aagcaaatgc atgaaaactt
      241 ccgctcccgg cgttcccagc cgggagtttt tttctatgct tattatccat gcattttcca
      301 aaagcttcaa cggcattcct gtgttttcgg acatctccct ttccgccgaa cagggaagct
      361 ttaccgtgct cctggggcct tcaggctgcg gcaagagcac cctcttcgac ctgctgatgg
      421 gagtcctccc cccggacggc ggccggatct cccttaatgg acgggaacgt cccgacctga
      481 gaggcgcggc agcctacatg gcccagaaag atcttctgct gccgtggaaa acgttgaggg
      541 aaaacgccct tctgcccgtg acagtgaggc gaaggcccac ggaagaagat ctccgcaggg
      601 tggaggacct cttttccctg ctggggctct caggccggga agactttttc cccggccagg
      661 tatccggagg gatggcccag cggtgcgccc tggcacgaac gatactgttc gacgcaccag
      721 tggcccttct ggacgagcct ctttccgccc tcgacgccat aaccaggcag actctcaggg
      781 aactgcttct cctcttgcag tcccgcttcg ggaaaaccat cctcatggtg acccacgatg
      841 tggaagaagc ccttctgctg gccgacacca tcctggtgct ctctccccct ccgatgagaa
      901 tcctcgaggt tttccggccg gaaggaacaa agaactcgag agaagaatcc ccctccttcc
      961 cgggaatgaa gcggcgcata ctgaaactcc tccgggaggg ctcccgatga ggcaggcggc
     1021 tcttcccctg ttgtttttct gcatattcct ggctgtctgg gagactctct gccgtttgtt
     1081 cgcccttccg ccctttcttc tgcccgctcc ttcgagggtg gttcatgttc ttgcggcgga
     1141 agctcctctc ctgttccggc atgg
//
LOCUS       DAOTHD010000850         1163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_146947_length_1163_cov_2.478339, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000850 DAOTHD010000000
VERSION     DAOTHD010000850.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146947_length_1163_cov_2.478339"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="P5219_11930"
     CDS             complement(<1..661)
                     /locus_tag="P5219_11930"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HRX27521.1"
                     /translation="MKKWLSAVLACVLGSLAFFIVSATTEEKEVVLSPVAPPAIEKEL
                     PSAPPAESFLSVPLSITAAEAERLLGNALGNPLYNVKGQKLDDSYGESAADVLVERTG
                     PVSVSLENGRARVTLPFRFESLVRWKGNILGISSSTKQEIFGGGRIRLEVKPTIDRDW
                     KIRLAGSLAVEWKEKPAVTILGQKVGIARFLSSILEERSGDLLRRAEEEINRSARLKE
                     RA"
     gene            1004..>1163
                     /locus_tag="P5219_11935"
     CDS             1004..>1163
                     /locus_tag="P5219_11935"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HRX27522.1"
                     /translation="MEQLFEGFLAITAKQAVMMGVGAVLLYLAIAKEYEPSLLLPMGF
                     GTLLVNIPF"
BASE COUNT          212 a          343 c          373 g          235 t
ORIGIN      
        1 gggcgcgctc cttcagccgg gcggaccggt tgatctcctc ctcggcccgg cgcaggaggt
       61 ccccggagcg ttcttcgagg atggaggaga ggaaacgggc gatcccgacc ttctggccga
      121 ggatggtgac ggccggcttt tccttccatt ccacggccag ggagccggcg aggcggatct
      181 tccagtccct gtcgatggtc ggcttcactt cgagcctgat cctgccgccg ccgaaaatct
      241 cctgctttgt gctcgaggag atgccgagta tgttcccctt ccaccggacg aggctttcaa
      301 acctgaaggg aagggtgacc ctggccctgc cgttttccag cgaaactgag accggtcccg
      361 tccgttccac gagcacgtcg gccgccgatt ccccgtaact gtcgtccagc ttctgcccct
      421 tcacgttgta gagggggttt cccagggcgt tcccgaggag acgctccgcc tccgccgctg
      481 tgatgctcag agggacggag aggaaggatt cggcgggcgg ggcggagggc agttccttct
      541 cgatggcagg aggggccacc ggggaaagga cgacctcctt ttcctcagtc gtggcggaga
      601 cgatgaaaaa cgcaagggac ccgagaacgc atgccaggac ggcagacagc cattttttca
      661 tggaaatacc tctcagcgga aaattcgttt tcttttttcc atccattata ctccgaaaga
      721 gagccctgcc ggaagaatgc gtcccggtcc cgggaccggg ggaccgtaat gaatccgcgg
      781 gcaccggcga cgctccccgt cgcctgcggg tacggacgtg cgggccgtca ttgacaatca
      841 ccttccaccg gatacaatga gtgctgcggg aacagatgcc gggaatcatg cgccgctttt
      901 ccgccggcgt tctgccccgg gcaatgggta tgtacggagg ccttcagcgg gaatatcctg
      961 cgctgggggt catttttctt ctgaacgaag gagagagaaa acgatggagc agctctttga
     1021 aggttttctt gccatcacgg caaaacaggc ggtgatgatg ggggtgggag cggtcctgct
     1081 ctacctggcc atagcgaagg agtacgagcc gtccctgctg ctccccatgg ggttcgggac
     1141 gctgctggtg aacatcccgt ttt
//
LOCUS       DAOTHD010000851         1159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_147686_length_1159_cov_2.829710, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000851 DAOTHD010000000
VERSION     DAOTHD010000851.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147686_length_1159_cov_2.829710"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="P5219_11940"
     CDS             complement(<1..730)
                     /locus_tag="P5219_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRX27523.1"
                     /translation="MNLPALRNFSVGSLDDAMVRALEEAAARCRRNAVTMVSLADSGH
                     PAGSLSSTEMYLAVYGVADLTPKNCSDVDRDYVSISHGHTSPGAYAALAEWGFFSSMD
                     AVAHFRQCGSPFQGHVERDVPGIDWGSGNLGQGLSAGVGFALAQKARKNGRRTYVLMG
                     DGGQTKGQSAEARRIAVKEKLTNLTALVDWNNIQISGTLESVMPADIPALWRADGWNV
                     LECDGHSFRELYAALRLSASCDRPT"
     gene            complement(867..>1159)
                     /locus_tag="P5219_11945"
     CDS             complement(867..>1159)
                     /locus_tag="P5219_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HRX27524.1"
                     /translation="MAVLPVGYADGYSRALSMKVDVLVGGKRVPHAGNICMDQMMVNV
                     TGMDVKVGDEVVLIGRQGGERISPEEIAAARNTINYEVPIMFLRRVPRVYIS"
BASE COUNT          216 a          364 c          369 g          210 t
ORIGIN      
        1 ccgtgggccg gtcacaggac gcgctcaggc ggagggcggc gtaaagttcc cggaaggagt
       61 ggccgtcgca ttccagcaca ttccacccgt cagccctcca gagggcgggg atgtccgcgg
      121 gcatgaccga ctcgagggtt cccgagatct ggatgttgtt ccagtccacg agagctgtga
      181 gattggtcag tttttccttg acggcgatcc ggcgggcctc ggcgctctgg cccttggtct
      241 ggcctccgtc ccccatgaga acgtaggtcc tcctgccgtt cttcctggcc ttctgggcca
      301 gggcgaaccc cacgcccgcg gaaaggccct ggccaaggtt gccgctcccc cagtcgatac
      361 ccggcacatc ccgttcgacg tgtccctgga agggggaacc gcactggcgg aaatgggcga
      421 ctgcgtccat ggaagaaaag aaaccccact ccgccagggc ggcgtaggct cccggggatg
      481 tatggccgtg gctgatggaa acatagtccc ggtcgacatc ggagcagttc tttggcgtca
      541 ggtcagccac accgtagacg gccaggtaca tttccgtgct cgagagggat ccggcgggat
      601 ggccgctgtc cgccagggag accatggtga cggcatttcg cctgcagcgt gcggcggctt
      661 cctcaagggc gcggaccatg gcgtcatcga gggagccgac ggaaaaattc ctcagtgcgg
      721 gaaggttcat tttcgtatcg cctccttgaa ttgtgacagg actgattata cccaagacgg
      781 gagaaaaagt caggaaaaag ggcggtcccg ccggaccgcc cgaaggtgcc ttgcatagcg
      841 gatgcccgca gggaatgctg cggccctcag gagatgtaga ccctcggaac ccgcctgagg
      901 aacatgatgg gcacttcgta gttgatggtg tttctcgcgg cggcaatctc ctccggggag
      961 atgcgctcgc ccccctgacg cccgatgaga accacctcgt cacccacctt cacgtccatg
     1021 ccggtgacgt tcaccatcat ctggtccata cagatgttcc cggcatgggg gacccgcttg
     1081 ccccctacca gaacgtccac cttcatggag agggcccggg aatagccgtc ggcatacccc
     1141 actgggagca ccgccatgg
//
LOCUS       DAOTHD010000852         1158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_147859_length_1158_cov_3.398912, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000852 DAOTHD010000000
VERSION     DAOTHD010000852.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147859_length_1158_cov_3.398912"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1158
                     /locus_tag="P5219_11950"
     CDS             <1..>1158
                     /locus_tag="P5219_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRX27525.1"
                     /translation="YAALRLGTSGDRPTVILCRTVMGRGVSFMENTPEFHGKAPRTEL
                     YVQAMKELGGDPSLLDEARKLRGSAQIPAGRHVAPSKADLDPGEPVTYGTDKKTDNRS
                     AFGTALADIGEKNYEKEGRTPVLVFDCDLAGSVKTGDFAKKCPDWFIQAGIQEHSTAT
                     AAGAASTAGVVSLWADFGVFGLSETYNQQRLNDINSANLKTVLTHVGLDVGEDGKTHQ
                     AIDYVGLLRNTFGWKLVVPGDPNQTDRATRWALGEAGCVCIAMGRSKLGVLADEQGDP
                     LFGGDYRFRYGEAVLVRPGKDGAIFALGAMTERAVKARDILREKHGLEVSVYCVSSPL
                     APDDGALREAAATGRILTCEDHSVNSGMGSILAARMTELGLSASFRAVGVHT"
BASE COUNT          214 a          386 c          381 g          177 t
ORIGIN      
        1 tacgcggctc tccgcctggg tacgtccggc gaccggccca cggtgatcct gtgccggacc
       61 gtcatgggca ggggggtgtc cttcatggag aatacgcccg aattccacgg aaaggctccc
      121 cggacggaac tctatgtcca ggccatgaag gagctcggcg gtgacccctc tcttctcgac
      181 gaagcgagga aactccgcgg gtcggcacag atccccgcag gaaggcacgt ggcgccctcg
      241 aaggcggacc tcgacccggg agaacccgtc acctacggaa ctgacaagaa gacggacaac
      301 cgttccgcct tcggcacggc cctcgccgac ataggggaga agaattacga aaaggaaggc
      361 aggacacccg ttctcgtctt cgactgtgac ctcgccggtt ccgtgaagac gggggatttc
      421 gcgaaaaagt gccccgactg gttcatccag gcgggaatcc aggaacattc caccgccacc
      481 gccgcggggg cagcttccac ggcgggcgtg gtttccctgt gggccgactt cggcgtcttc
      541 ggcctgtccg agacgtacaa ccagcagcgg ctgaacgaca tcaactccgc caacctcaag
      601 acggtgctca cccacgtggg ccttgacgtg ggcgaggacg gcaagaccca ccaggccatc
      661 gactacgtgg ggctgctccg gaacaccttc ggctggaagc tcgtggtgcc cggagatccc
      721 aaccagaccg acagggccac ccgctgggcc ctcggcgagg cggggtgcgt ctgcatcgcc
      781 atgggccgga gcaagctcgg cgtcctggcc gatgaacagg gagaccctct cttcggcggc
      841 gactatcgct tccgctacgg tgaagcggtt ctggtgcgcc ccgggaagga cggcgccatc
      901 ttcgccctcg gcgccatgac ggagcgggcc gtgaaggccc gggatatcct ccgggagaaa
      961 cacggcctgg aggtttcggt atactgcgtt tccagccccc tcgccccgga cgacggggcc
     1021 ctccgggaag cggcggccac gggccggata ctcacctgcg aggaccactc ggtgaacagc
     1081 ggcatggggt ccatccttgc cgcccggatg actgaactgg ggctcagcgc atccttcagg
     1141 gccgtgggtg tgcacacc
//
LOCUS       DAOTHD010000853         1158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_147886_length_1158_cov_2.746147, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000853 DAOTHD010000000
VERSION     DAOTHD010000853.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147886_length_1158_cov_2.746147"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="P5219_11955"
     CDS             complement(<1..675)
                     /locus_tag="P5219_11955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRX27526.1"
                     /translation="MIEPTSRFWQLFLELFESLPRQGPGNRACTERALGLCDGLPPFP
                     KVADLGCGVGGQTLHLAAMTRGTIVAVDLHEPSISNLRETVSSLGLSERIFPMVADMA
                     DTGLLPGSFDLVWSEGALYSIGMERALSVCRELLRPGGFLAFTDAVWLRDDPPSEVKE
                     SFDLDYPSMGRTADILALLEQTGFSPAGHFTLPDEAWWMHFYTPMEKRIAEMRKQYEA
                     DEEALAV"
     gene            complement(929..1093)
                     /locus_tag="P5219_11960"
     CDS             complement(929..1093)
                     /locus_tag="P5219_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27527.1"
                     /translation="MFKKLVFMSGKPFWLLPEGDFLAVEQKNGRIVDPVLFRKSNDRW
                     NSEVTSEQGN"
BASE COUNT          235 a          345 c          325 g          253 t
ORIGIN      
        1 gacagcgagg gcttcttcgt cggcttcgta ttgtttccgc atctcggcga tacgtttttc
       61 catgggagtg tagaagtgca tccaccaggc ttcgtccggc agggtaaaat ggcctgccgg
      121 ggagaacccg gtttgttcca gcagcgccag aatgtcagcc gtccttccca tggacgggta
      181 atcgaggtcg aagctctcct tcacctctga cggaggatca tccctgagcc acacggcgtc
      241 tgtgaaggca aggaatcccc ccgggcggag aagttcccgg cagactgaca gggcccgttc
      301 cataccgatg gagtacagcg ctccctctga ccagaccagg tcaaagcttc ccgggagaag
      361 ccccgtatcg gccatatcgg cgaccatggg gaaaatccgt tccgagagcc cgagggagga
      421 caccgtctcc ctgaggttgc tgatgctcgg ttcatgaagg tccacggcga cgatggttcc
      481 cctggtcatg gctgcaagat ggagggtctg tcctccaaca ccgcacccga ggtccgcaac
      541 tttcgggaag ggtggcaggc cgtcgcacag cccgagggcc ctctcggtac aggcccggtt
      601 tccgggcccc tgccgcggaa gtgactcgaa cagctccagg aacagctgcc agaaccggga
      661 cgtcggttca atcatggttc tccctccttc ggatattact gggacataag aatctatccg
      721 gggtccgttc cgcaggaaaa tcctcccggt tgctgaaaac acggtcaccc ccggcgtgta
      781 ccctctctca ccgattcaat tgtcggggca ttgtccttgg ccgtcaatac tcatggagag
      841 gcagttcctg ccctcctttt ctgaataaaa aaagctcctc ccctgatggg gaggagctta
      901 cgctttcaca ggggcgaaag ggaaattctt agtttccctg ttcgctggtg acttcgctgt
      961 tccaccggtc gttcgacttg cggaaaagca ccgggtcgac aatcctgccg ttcttctgtt
     1021 ccacggcgag gaaatcgccc tccgggagaa gccagaaggg ctttcccgac ataaacacaa
     1081 gttttttgaa catggttgta cctctttctc tttctgaatt ccgaaaaaac ggcgcctcaa
     1141 aggggatgtc tcggttcc
//
LOCUS       DAOTHD010000854         1156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_148291_length_1156_cov_2.955495, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000854 DAOTHD010000000
VERSION     DAOTHD010000854.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148291_length_1156_cov_2.955495"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="P5219_11965"
     CDS             complement(<1..882)
                     /locus_tag="P5219_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27528.1"
                     /translation="MMSSQEHERKKIRSAVIATAALLVVSTAVTWGMDHYRWSGAKSE
                     ARKLVAASQYADALKVVDSHGKGYIFPDPIPLVPSVLRGGADKESFRSGILKAYEQAQ
                     FNNLQPLIEKIDTSRMPDVKSAEYLDASEKVEEYLANPDFRKINQANFDKIYSLRWYF
                     EAGQALHGKTAGSADGASDSGEALNLVESWLGFLPKLPEQWKSEGARKAGELFAFWAD
                     MLSPDAGIESVEDFLASAQKLSGNPNATDELKKSIAERETAWKQMIADKWTDRGAKWV
                     QEASALSPEEGIGKILTF"
BASE COUNT          214 a          314 c          310 g          318 t
ORIGIN      
        1 aaaggtcagg atttttccga tcccttcttc cggcgaaagg gcggaagcct cctggaccca
       61 tttcgctcct ctgtcggtcc acttgtctgc aatcatttgt ttccaggcag tttccctttc
      121 tgcaatgctt tttttcaact catccgtggc gttcgggttg ccggagagtt tttgtgcaga
      181 tgcaagaaag tcctctacgg actcgattcc ggcatcgggg gaaagcatat cagcccagaa
      241 ggcaaagagt tcccctgctt tccgggctcc ctcgctcttc cactgttcgg gaagcttcgg
      301 caggaagccg agccagcttt ccacaaggtt gagggcttca ccggaatcgc tggccccgtc
      361 ggcgctcccc gccgttttgc cgtggagggc ctgccctgcc tcaaagtacc atcggagaga
      421 atagattttg tcaaagttgg cctggtttat tttgcggaag tccgggttgg cgaggtactc
      481 ctccactttt tccgatgcat ccagatactc tgcggatttt acatcaggca ttctggatgt
      541 gtctattttc tctatgaggg gctgcaggtt gttgaactgc gcctgttcat aggctttcag
      601 gattccgctg cggaaggatt ccttgtccgc cccgccacgc aggaccgagg gaacgagagg
      661 gatggggtca gggaagatat atccctttcc gtgtgaatcc accaccttca gggcgtctgc
      721 atattgcgag gctgccacaa gtttccgggc ctcgcttttc gcgccgctcc acctgtagtg
      781 gtccatgccc caggttaccg ccgtggagac cacaagaagc gctgctgtcg ctataacggc
      841 tgatctgatc ttttttcgtt cgtgctcctg gctgctcatc attttgtaaa gttcctccat
      901 tggttttatg acattctgcg gttcatattt ttgcggcagt ttttttggct caggccagac
      961 cccgatggga atagccttga aggcgatgac ggtctccctg tcaccggaaa gacaggccga
     1021 aagagccgga aatttttggc cttctttgac caggatcgcg gtcattttgt cagtaagctg
     1081 ctcaacggaa acgtcaggaa ccgtgtcttc ctgtttacaa agggagacac ggttcctgac
     1141 gtttccgttg agcagc
//
LOCUS       DAOTHD010000855         1151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_149231_length_1151_cov_4.012774, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000855 DAOTHD010000000
VERSION     DAOTHD010000855.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149231_length_1151_cov_4.012774"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="P5219_11970"
     CDS             complement(<1..346)
                     /locus_tag="P5219_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRX27529.1"
                     /translation="MNPRELLRYLENGKRVLILDGGMGTLLAEKGWNPPVLPEEMNLG
                     SPEIVESIHASYRSAGADILETNTFGGSPVKLAARGLGARAKEINARAAALARSAAGS
                     GGLVAGSMGPLGE"
     gene            479..>1151
                     /gene="dapF"
                     /locus_tag="P5219_11975"
     CDS             479..>1151
                     /gene="dapF"
                     /locus_tag="P5219_11975"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRX27530.1"
                     /translation="MIPFTKMQGNGNDFIVLDNMSGQYPAEFLSRAAVSYCRRRQSLG
                     ADGVLVAETSPDADFTMRLFNADGSEGEMCGNGARCIARYAFEKNIAGRIQRFSTLAG
                     IMEATVDSPFVDLRMGTVSLEGGWFNRPLSVAGETFQASFLRVGVPHAVLFCETEPSR
                     ERMVQVGRTVRHDRNLFPDGANVNFVFGDGEAAIRSVTYERGVEDLTDSCGTGSTASA
                     ICRLLR"
BASE COUNT          224 a          329 c          348 g          250 t
ORIGIN      
        1 gttcccccag gggacccatg gagccggcga caagcccacc ggatccggcc gcggaacggg
       61 ccagggctgc cgcccgtgcg ttgatttcct tcgcccgtgc gccaagcccc cgtgccgcga
      121 gcttgacggg gctgccgccg aaggtgttgg tttcgagaat gtccgccccg gcggaccggt
      181 aggatgcatg aatggattcc acgatttcgg gactgccgag gttcatttcc tcgggcagaa
      241 cgggaggatt ccagcccttt tccgcgagaa gcgttcccat gcctccatcg aggatcagga
      301 cccttttccc gttttccaga taccggagca gttccctggg attcatgacg tttcctcctt
      361 cagacccgtg taaatacatc aatatagcag aaaactgcgg ttgtatcatg gagtaaaatt
      421 tggtagcgta gaatagtatt gtaaacgagt aaaaaactat cgtccggggg agataaccgt
      481 gattccattt accaaaatgc agggaaacgg caacgatttc attgtgcttg acaacatgtc
      541 gggccagtat ccggcggaat tcctttccag ggcggccgtt tcgtactgca gaaggcgtca
      601 gtccctcggg gctgacggtg tgcttgtcgc ggaaacatcc ccggatgccg atttcaccat
      661 gcgccttttc aatgccgacg gcagcgaagg ggaaatgtgc gggaacggtg cccggtgcat
      721 cgcccggtat gcctttgaaa agaacatcgc gggcaggatc cagcgttttt ccacccttgc
      781 gggcattatg gaggcaacgg tggattcacc ctttgtcgat ctccggatgg gaaccgtctc
      841 ccttgaggga gggtggttca accggcctct ctccgtggcc ggagagacgt ttcaggcctc
      901 ttttctccgg gtgggcgttc cccacgctgt cctgttctgt gaaacggaac cgtcccggga
      961 gcgcatggtc caggtgggaa ggacggtccg ccacgacagg aacctcttcc ccgacggcgc
     1021 gaacgtgaat tttgtcttcg gggacggtga ggcggccata cggtccgtta cctatgagag
     1081 aggagtggag gatctcaccg attcctgcgg caccggatcc accgcgtccg caatctgccg
     1141 gcttctccgg a
//
LOCUS       DAOTHD010000856         1147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_150113_length_1147_cov_2.431319, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000856 DAOTHD010000000
VERSION     DAOTHD010000856.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150113_length_1147_cov_2.431319"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="P5219_11980"
     CDS             complement(<1..524)
                     /locus_tag="P5219_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4/precorrin-4
                     C(11)-methyltransferase"
                     /protein_id="HRX27531.1"
                     /translation="MKLSERPILFVGAGPGDPELLTLKGKKALEEAGLVLYAGSLVNA
                     ALLDFCSPGCERADSSGLSLEEQVALMADAAGRGVPVVRLHTGDPSLFGAVAEQKRLL
                     EEQGLSVKFIPGVSSFQAAAAALGIQYTVPGGNQTVICTRRAGRTPVPPEEDLRRLAA
                     VGTTMVVFLSADQA"
     gene            complement(511..>1147)
                     /gene="cbiT"
                     /locus_tag="P5219_11985"
     CDS             complement(511..>1147)
                     /gene="cbiT"
                     /locus_tag="P5219_11985"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="precorrin-6Y C5,15-methyltransferase
                     (decarboxylating) subunit CbiT"
                     /protein_id="HRX27532.1"
                     /translation="LGPGPLPPQTLGVLPDGWFVRTPGVPLSKAVCRALAASLLHPLD
                     GQAVLEIGAGSGGITVELARRTGTGTVFAVERSADALDAARNNAERAGCLSSIRFVEG
                     QAPEALASLPRCTRVVVGGHGGAVEAVLEAAWEKLLPGGRLLATANMPSSADRAWKTL
                     KSLGSRPEVLHVAPSSSAEAGGSWMLTAANPVFLVYADRNADKDGTIHETL"
BASE COUNT          173 a          388 c          410 g          176 t
ORIGIN      
        1 tcggcctggt cggcgctcag gaagaccacc atggtggtcc cgacggcggc gaggcggcga
       61 agatcctcct ccgggggcac aggcgtccgt ccggccctgc gggtgcagat caccgtctgg
      121 tttcccccgg gaacggtgta ctggatgccg agggctgcgg cggcggcctg gaagctgctg
      181 acgccgggga tgaacttcac ggacaggccc tgttcctcca ggaggcgctt ctgttccgcc
      241 acggcgccga agaggctcgg gtcccccgtg tggaggcgca ccacaggcac tccccggccc
      301 gcggcgtccg ccatgagggc cacctgctcc tccagggaga ggccggagga gtccgcccgt
      361 tcgcaccccg gagagcagaa atcgagaaga gcggcattta ccaggctgcc tgcgtagagc
      421 accagccccg cttcctcgag ggcctttttc cccttgaggg tgaggagttc cgggtctccg
      481 gggcccgccc ccacgaagag tatgggtctt tcagagagtt tcatggatcg taccgtcctt
      541 gtctgcattc ctgtccgcat agacgagaaa cacggggttc gccgccgtga gcatccacga
      601 accgcccgcc tccgccgagg aggagggcgc gacgtggagc acttcggggc ggcttccgag
      661 gcttttgagg gttttccatg ccctgtcggc cgatgagggc atgttcgccg tcgccaggag
      721 ccgtccgccg ggcaggagct tttcccaggc ggcttcgaga acggcctcga cggccccgcc
      781 gtggccgccc accacgaccc gggtgcaccg ggggagggaa gccagtgcct ccggtgcctg
      841 tccttccacg aagcgtatgg aggagaggca cccggcccgt tccgcgttgt tccgggcggc
      901 gtcgagggcg tccgccgaac gctccacggc gaagaccgta cccgttcccg tccgccgggc
      961 cagctccacg gtgatgccgc cggagcccgc cccgatctcg aggacggcct gcccgtccag
     1021 gggatggagc agggaggcgg ccagggcccg gcagaccgcc tttgataggg gaactcccgg
     1081 ggtccggacg aaccatccgt cggggagtac accgagggtt tggggcggaa ggggtcccgg
     1141 accgaga
//
LOCUS       DAOTHD010000857         1146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_150273_length_1146_cov_3.067828, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000857 DAOTHD010000000
VERSION     DAOTHD010000857.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150273_length_1146_cov_3.067828"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /locus_tag="P5219_11990"
     CDS             complement(<1..1069)
                     /locus_tag="P5219_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27533.1"
                     /translation="MMDIPPMENFFGLSGRLAEIFPSFEYRKQQADLAEEARRTLLGG
                     PGEILAAEAPPGVGKTFALLIPAMTEAAATGKKILFLTAGIPLQEQLIHKDLPALNRV
                     LGLSLPFGLLKGKGNYACLVRAAEASESGRGGFLSFGDGGAASMLIAQWLETTETGDL
                     SELRLPPDSPAVAGVAASSRACLGFRCPFKDRCFSRKALREAQDWSVVVANYHLFFSY
                     LLGAGKPFPVPCDILICDEAHRIPEAARSSMAMSVSADDFARLLRHRAVSEGASLLEH
                     GSSAGRIAALSGEIRQETVRFFDLLEVKVPAKKASFTEPSEELRHQQAVVSEKVHELL
                     RFFAPLVDGQEEPDPEQSAAAV"
BASE COUNT          234 a          339 c          343 g          230 t
ORIGIN      
        1 acaccgccgc ggcggactgc tcggggtcag gttcttcctg cccgtccacg agaggagcga
       61 agaacctcag aagctcatgt accttctctg aaacaaccgc ctgctggtgc ctgagctctt
      121 cactgggctc cgtgaaggac gcctttttcg caggcacctt gacctcgagc aggtcgaaga
      181 aacggaccgt ttcctgccgt atttctcccg acagggcggc gatcctgccg gcggatgaac
      241 catgctcgag gagcgaagca ccttccgata cggcacggtg ccggagcagc ctggcaaagt
      301 cgtcggcgga caccgacatg gccatggagg atctagcggc ctctgggatc ctgtgggcct
      361 cgtcgcagat cagaatgtcg caggggacgg gaaagggctt tcccgcccca agcagatagg
      421 agaaaaaaag gtgatagttt gccacgacta cagaccagtc ctgggcctcc ctgagagcct
      481 tcctggaaaa gcacctgtcc ttgaaggggc agcggaagcc gagacaggcc ctcgatgagg
      541 ccgccactcc agccaccgca ggagaatcag gaggcaggcg cagttccgag agatcccccg
      601 tttccgtggt ctcgagccac tgggcgatga gcatggatgc cgcgcctccg tccccgaagg
      661 aaagaaagcc tcccctgccg ctttccgagg cctcggctgc tctgaccagg caggcgtagt
      721 ttcccttccc cttcagtagg ccgaagggaa gagaaaggcc gagcaccctg ttgagtgcgg
      781 gaagatcctt gtggatgagc tgctcctgga ggggaatgcc ggcggtaagg aagaggattt
      841 tctttcccgt ggcagccgct tctgtcatgg ccggaatgag cagggcaaag gttttcccta
      901 ctcccggagg cgcctcggcg gcaagaattt cacccggccc cccgagaagg gttcgtctcg
      961 cttcctcggc gaggtcagcc tgctgtttcc tgtattcaaa cgaagggaat atctctgcga
     1021 gccttccgga caggccgaag aagttctcca tagggggtat gtccatcacg attttctctc
     1081 ttcctttttc gggacatttt tttccatccc ttgctaatat aacagaaatc gtgctagaat
     1141 gtcgtg
//
LOCUS       DAOTHD010000858         1146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_150285_length_1146_cov_2.898258, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000858 DAOTHD010000000
VERSION     DAOTHD010000858.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150285_length_1146_cov_2.898258"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(250..>1146)
                     /locus_tag="P5219_11995"
     CDS             complement(250..>1146)
                     /locus_tag="P5219_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27534.1"
                     /translation="VFPEGKEEREEREEREEREEREEREEREEREEREEREEETGRPS
                     RWYSAPLGNGRNLLVTWSEQRARKDGSDRERLLGRLRKKLQGKKTLPGKSLVTNRGTN
                     KYLSLENTDGQDRYILDEEKILRDSRWDGLHGVITDMSAESAAEAQDILAHYGSLWRI
                     EESFRVNKHDLSIRPVYHWAPRRIEAHIALTYLAFALLRHLQHRVAVRQNAVMSARAI
                     RTALLDVQSTLMKDEETGKLYRFPKAMSESARKIYRSLGLVRGLGNTEILSVRKYRNR
                     KGIRSAGTDGDGTMEEEGTGGN"
BASE COUNT          161 a          359 c          326 g          300 t
ORIGIN      
        1 gtcccccgga ttctgctccg cagcggcgga gcgagaataa aatacacctt ttatcccgac
       61 ttcgccgggt ttgaaacggg aagccttgca aacacaggat tgtgcatcgt tggtgggcac
      121 tacacagccc ctcttttcaa ttctgtgaaa agaggggctg tcgtttgagg cggatttttc
      181 ggtctcaccg tgtctcgggg ctccgaaagg tatttcgaaa ggtgaacagg agatctgctc
      241 ccggctcgtt cagttcccgc ccgtcccttc ctcctccatc gtcccgtctc cgtccgtccc
      301 ggcgctccgg atccctttcc ggttcctgta tttcctcacc gacaggattt ccgtgtttcc
      361 cagtccccgg acgaggccca gggaacggta gatcttccgg gcgctctcgc tcatggcctt
      421 cgggaaccgg tagagtttcc ccgtctcttc gtccttcatg agggtggact ggacatccag
      481 cagggcggtc cggatggctc gggcgctcat caccgcgttc tgccggaccg ccacccggtg
      541 ctggaggtgg cggaggaggg cgaaggcgag gtaggtgagg gctatgtggg cctctatcct
      601 gcggggagcc cagtgatata ccgggcggat ggagaggtca tgcttgttca cccggaagga
      661 ttcttcgatg cgccacaggc tcccgtaatg ggcgaggatg tcctgcgctt ccgcggcact
      721 ttccgcggac atgtcggtga tgactccgtg aaggccgtcc caccggctgt cccggaggat
      781 cttttcttcg tccaggatgt agcggtcctg tccgtctgtg ttttcaaggg agaggtattt
      841 gttcgttccc cggttggtga cgaggctttt tcccggaaga gtcttcttcc cctgcagttt
      901 tttccgcagt cggccgagaa gccgttcccg gtcggagccg tccttccggg ctcgctgttc
      961 actccaggtg acgagcaaat tcctcccgtt cccgaggggg gcggagtacc aacgggaagg
     1021 tctgccggtc tcttcttccc gttcttcccg ttcttcccgt tcttcccgtt cttcccgttc
     1081 ttctctctct tctctctctt ctctctcttc tctctcttct ctctcttcct tcccctccgg
     1141 gaagac
//
LOCUS       DAOTHD010000859         1140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_151495_length_1140_cov_3.458065, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000859 DAOTHD010000000
VERSION     DAOTHD010000859.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151495_length_1140_cov_3.458065"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /gene="lpxI"
                     /locus_tag="P5219_12000"
     CDS             complement(<1..617)
                     /gene="lpxI"
                     /locus_tag="P5219_12000"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF037151.2"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HRX27535.1"
                     /translation="MKTVTGPAALIAGDGALPEETARQMAGAGLDVTAYSFSGGAEDR
                     FPFLPKDRVLSLLSLPGGEGKLSLQALLADLRARGIRSVCMAGLIPKTIMYGAGADTS
                     LLGMLSSDGNDDHSLLGRIVAAFESFGIRVLPYTGFVRESLAAEGVLAGREPSEQERE
                     DVSCGKGILSVTLPLSFGQSVVVARGAVVAVEAMEGTDEMVRRAGG"
     gene            complement(639..>1140)
                     /locus_tag="P5219_12005"
     CDS             complement(639..>1140)
                     /locus_tag="P5219_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRX27536.1"
                     /translation="NGIWGDGEWWLQDGQVFEVTREGRVNPLLRFQRQKLQLSLTPAQ
                     VEKASRKPSEMSSTELLVQIALLSKEGRNLAPLKVMFHLRLALPWASVVLAVLGASLG
                     VRSSRAGPGIGFGLSVLIVFAYYVTMSFSRALGEAGYLFPVIAAWIPNVSFLLVGGYF
                     ARRANG"
BASE COUNT          209 a          377 c          374 g          180 t
ORIGIN      
        1 ccccccgccc ggcggaccat ttcgtcggtg ccttccatgg cttccacggc gaccaccgca
       61 ccccgggcca ccacgacgga ctggccgaag gagaggggaa gggtgacgga caggatcccc
      121 tttccgcagc tcacatcctc ccgctcctgt tccgagggtt cccggcccgc gagaacccct
      181 tccgccgcca ggctctcccg gacaaatccc gtatagggga gaacccggat gccgaaactc
      241 tcgaaggccg ccacgatgcg gcccaggaga ctgtgatcgt cgttcccgtc cgaagagagc
      301 atgccgagga gagaagtgtc cgcccctgcg ccgtacatga tcgtctttgg gatgagcccc
      361 gccatgcaga cggaccgtat gcccctggcc cggaggtcgg cgaggagggc ctgaaggctc
      421 agcttccctt cgcccccggg gagggagagc agggaaagca cccggtcctt cggaaggaac
      481 gggaaccggt cttccgctcc gcccgagaag gaataggcgg tgacgtccag cccggctccg
      541 gccatctgcc gggcggtttc ctccggaagg gctccatcgc cggcgatgag ggcggcggga
      601 cctgtcacgg ttttcatgag atcaatcctt tccggctctc agccgtttgc ccggcgggcg
      661 aagtatcccc ccacgaggag gaaggacacg ttgggtatcc aggcggcaat gaccgggaac
      721 aggtatccag cctcgccgag ggcccggctg aaggacatgg tgacataata ggcgaacacg
      781 atgaggacgc tgaggccgaa gccgatcccc gggcccgccc tgctcgaccg gacgccgagg
      841 ctggcgccga gcaccgcaag gaccacgctt gcccagggaa gggcgagtcg gagatggaac
      901 attaccttga ggggggcaag gttccggccc tcttttgaca ggagggctat ctggacgaga
      961 agttccgtgg aactcatctc ggagggcttc cgggaggctt tttccacctg ggcgggcgtc
     1021 aggctcagct ggagcttctg ccgctggaac cggagaagag ggttaacccg cccttcccgg
     1081 gtgacctcga agacctgtcc gtcctgaagc caccattcgc cgtcccccca gattccgttg
//
LOCUS       DAOTHD010000860         1140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_151503_length_1140_cov_3.211060, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000860 DAOTHD010000000
VERSION     DAOTHD010000860.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151503_length_1140_cov_3.211060"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1140)
                     /locus_tag="P5219_12010"
     CDS             complement(<1..>1140)
                     /locus_tag="P5219_12010"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HRX27537.1"
                     /translation="KFTAPKVGGLIDDLLPFAGSLPPDSDDGCLIRAADREYRKAVRV
                     PADFLAAFSEHSAVSYQTWTEARPADDFRRVLPLLEKTLDLSRRLADFFPGYEHIADP
                     LIDFSDYGMKAGSVRKVFGELREQLVPLVRAAVSGEPADDSCLKGHFPKDRQLDFGKM
                     VIGTFGYNFDRGRQDLTHHPFETRFSVGDVRITTRIDEGNFAYGFFSTTHESGHALYE
                     QGVDPALEGTLLAEGTSSGVHESQSRTWENLVSRSLPFWEHFYPKLQKVFPDRLGTVP
                     LETFYRAVNRVEPSLIRTEADELTYNLHVMIRFELELKMLEGSLEVKDLPEAWNAAYL
                     ENLGIAPEDNADGCMQDVHWFGGTIGGAFQGYTLGNILSAQFFEKA"
BASE COUNT          200 a          356 c          370 g          214 t
ORIGIN      
        1 gctttttcaa aaaactgggc ggagaggatg ttccccaggg tgtacccctg gaaggccccg
       61 ccgatagtgc cgccgaacca gtggacgtcc tgcatgcagc cgtcggcgtt gtcctcgggc
      121 gcgatcccga gattctccag gtacgcggcg ttccaagcct ccggcaggtc tttcacttcc
      181 aggcttccct caagcatctt cagttccagc tcgaagcgga tcatgacgtg aaggttgtag
      241 gtcagctcgt cggcctcggt gcggatcagg gagggttcca ccctgttcac cgcccggtag
      301 aaggtctcca ggggaacggt tcccaggcgg tccgggaaga ctttctgcag cttcgggtag
      361 aagtgttccc agaagggcag gctccgggag accaggtttt cccacgtgcg ggactgggac
      421 tcgtgcactc ccgagctggt cccctccgcc agcagagttc cctccagggc ggggtccact
      481 ccctgctcat acagggcgtg ccccgactcg tgggtggtgg agaagaaacc gtaggcgaag
      541 tttccttcgt cgatccgggt ggtgatgcgc acgtcgccca cggaaaaccg agtctcgaag
      601 ggatggtggg tcaggtcctg ccgtcccctg tcgaagttgt agccgaacgt cccgataacc
      661 atcttcccga agtcgagctg ccggtctttg gggaaatgcc ccttcaggca cgaatcgtcg
      721 gccggttccc cggacacggc ggcccgtacc agcggcacga gctgttccct gagctccccg
      781 aagacctttc ggacgctccc cgccttcatg ccgtagtcgg agaagtcgat gagcgggtct
      841 gcgatgtgct catatccggg gaaaaagtcc gccaggcgtc ggctcaggtc cagggttttc
      901 tccagcaggg gaaggacccg cctgaaatcg tcggcggggc gggcctccgt ccaggtctgg
      961 taagagaccg ccgagtgctc tgaaaaggcg gccagaaaat ccgcaggcac acggacggcc
     1021 ttccggtatt cccggtccgc cgcccggatc aggcagccgt cgtccgaatc ggggggaagg
     1081 cttccggcga aggggagcag atcgtcgatc agcccgccca ccttcggggc ggtgaacttt
//
LOCUS       DAOTHD010000861         1140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_151524_length_1140_cov_2.793548, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000861 DAOTHD010000000
VERSION     DAOTHD010000861.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151524_length_1140_cov_2.793548"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..491
                     /locus_tag="P5219_12015"
     CDS             57..491
                     /locus_tag="P5219_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HRX27538.1"
                     /translation="MHKFPVLKDGVNEKLLEKVRKKVDACLSGLFDKEDLVKVDGMTS
                     FSFGSATVNVTVVPWHSGDVLVKVFSYLAENVKTKKAATEFLRLNADVPLGAFSIAFD
                     NTVMFSCSLPGANVDTSEITGALQTVAAYADQYDDILKEMYR"
     gene            520..>1140
                     /locus_tag="P5219_12020"
     CDS             520..>1140
                     /locus_tag="P5219_12020"
                     /inference="COORDINATES: protein motif:HMM:NF023899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006996 - organelle organization [Evidence
                     IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIDE domain-containing protein"
                     /protein_id="HRX27539.1"
                     /translation="MNMIALAAGGLLAAGGAYLAFVQKKKTEGMLTELKFMKATPLQE
                     LHGTWKSLCDEGMGDGFRDFIETNGAAATDGELVAPYSGMPCAYYEATVLREYEKQET
                     STDKDGKVTTNRTRGSETVSSQKSSSPLYVSDGDVRVGIDLDGAALHLKDGADRFEPY
                     ESSKTYTFFGVRFSAPSGIRTLGFKYREKLIPLGHPLYVAGEARQSA"
BASE COUNT          259 a          315 c          356 g          210 t
ORIGIN      
        1 gctcgaggcg gaaatggcga agctcatgga aaaaatgaat gcggggggtg gcacgggtgc
       61 ataagtttcc ggtgcttaag gacggagtga acgaaaagct cctggaaaag gtcagaaaaa
      121 aggtggacgc ctgtctttcc gggctcttcg acaaggagga tctcgtgaaa gtggacggca
      181 tgacgtcctt ttccttcgga agcgcaaccg tcaacgtcac ggttgtcccc tggcattccg
      241 gcgacgtcct ggtgaaggtc ttttcgtacc tggccgagaa cgtgaaaaca aagaaggcgg
      301 cgacggaatt cctgaggctg aacgctgacg ttcccctggg ggccttttcc attgcctttg
      361 acaacaccgt catgttctcc tgctcccttc cgggggcaaa cgtcgacaca agcgaaatca
      421 caggtgccct gcagaccgtg gcggcctatg cggaccagta tgacgacatt ctgaaggaga
      481 tgtaccggta gtccttagct gaccaaagga ggggatggaa tgaatatgat tgctcttgct
      541 gcaggcggtc tcctggcggc ggggggggcg tacctcgcct tcgtccagaa gaagaaaacc
      601 gaaggaatgc tcacggagct caagttcatg aaggccacgc cgctgcagga acttcacggc
      661 acatggaagt ccctgtgcga cgagggcatg ggggacggtt tccgggattt catcgagacc
      721 aacggtgccg ccgccactga cggtgagctt gtcgctccct acagcggcat gccctgcgcg
      781 tactacgagg caaccgtcct cagggaatac gagaagcagg aaacgtccac cgacaaggac
      841 ggcaaggtga cgaccaacag gacgaggggg tctgagaccg tctcgtccca gaagagctcc
      901 tctcccctct atgtatccga cggcgacgtg agggtcggca tcgacctcga cggagcggcc
      961 cttcatctca aggacggcgc cgacaggttt gaaccctatg aaagcagcaa aacatacacg
     1021 ttcttcggcg tccggttctc ggctccgtcg ggaatccgga cgctcggctt caagtacagg
     1081 gagaaactca ttcccctggg acacccactc tacgtggcgg gggaggcccg gcagtcggcg
//
LOCUS       DAOTHD010000862         1140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_151544_length_1140_cov_2.554839, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000862 DAOTHD010000000
VERSION     DAOTHD010000862.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151544_length_1140_cov_2.554839"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1140
                     /locus_tag="P5219_12025"
     CDS             <1..>1140
                     /locus_tag="P5219_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27540.1"
                     /translation="ISLVKEGKPLFSAGFLVVKLNLMSLLSKSPKLSVVSVGGVTTDA
                     DALAEQISRISFEGGGEGEIPVETVRLVDSTVTSKWAKADITSLGLSFSGKDIDADLD
                     LAVNGVPIKGKTAVLVDGKAVDVKSMALNVGKGSVSASGKVSPRLAVSGAMKDLDLKE
                     LIAFWPVVPSDGFDGKVSLNFRGEGEWNAPSLAGDIEYSGKSVLGYPVDSVRAKWALG
                     ADTLSVTDIDARVLAMPLSGGMSLAFAPGKPPVLDLDLAGTSIKVAELKKLYPDMGDL
                     SGEIDKFTVKLSGAADALNGTVEFSAPALGAFGYSVTNSAAQVKINPKAATVSAKSNF
                     EGAPITAQGTVTDYMTSPKLNLTGNLRSFNLAKAAALVPDLKNLAL"
BASE COUNT          221 a          351 c          349 g          219 t
ORIGIN      
        1 gatcagcctc gtaaaggagg gaaaacccct cttttccgcc ggttttctcg tggtcaagct
       61 caacctcatg tcccttctct cgaagtctcc gaagctgagc gtggtctcgg tgggtggagt
      121 aaccaccgat gccgacgccc tggcggagca gatctcccgg atcagcttcg agggcggcgg
      181 cgaaggggag atcccggtgg agacggtccg tctcgtggac agcaccgtga cgtcgaaatg
      241 ggcgaaggcg gacatcacca gcctcggtct gtcgttttcg ggcaaggata tcgacgcaga
      301 ccttgacctt gccgtcaacg gcgttcccat caagggaaag actgccgtcc tggtggacgg
      361 aaaggctgtg gacgtgaaat ccatggcgct gaacgtgggg aaaggctccg tttcggcctc
      421 gggaaaagtt tccccacgtc ttgccgtgag cggtgccatg aaggatctgg acctgaagga
      481 gctcatcgcc ttctggccgg tggttccctc agacggtttt gatggaaagg tgtccctcaa
      541 cttccggggc gagggtgaat ggaatgcccc gtccctcgcg ggagacattg agtacagcgg
      601 aaaatccgtt ctcggctatc ccgtggacag cgtccgggcc aagtgggctc tcggcgccga
      661 cacactttcc gtcacggaca tcgacgcccg ggtcctcgcc atgccccttt ccggaggcat
      721 gagcctcgcc ttcgctcccg gaaaaccccc ggtgcttgac cttgatctcg cggggacgtc
      781 catcaaggtt gccgagctga agaagctcta tccggacatg ggcgaccttt ccggcgaaat
      841 cgacaagttc acggtgaagc tctccggggc cgcggatgcg ctgaacggca cggtggagtt
      901 cagcgctccc gccctgggcg ccttcggcta cagcgtcacc aactcggcgg cccaggtgaa
      961 gatcaatccc aaggcggcaa cggtgagtgc gaagagcaat ttcgaaggag cacccatcac
     1021 ggcccaggga acggttaccg actacatgac ttccccgaag ctgaatctca ccggcaatct
     1081 ccggtccttc aacctcgcga aggcggctgc ccttgtccct gatctgaaga acctcgccct
//
LOCUS       DAOTHD010000863         1140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_151606_length_1140_cov_1.922581, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000863 DAOTHD010000000
VERSION     DAOTHD010000863.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151606_length_1140_cov_1.922581"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="P5219_12030"
     CDS             complement(<1..323)
                     /locus_tag="P5219_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27541.1"
                     /translation="MSRSFAVFFSLYMLIYGSMHVFVYFKLRSAIGRGWKWTALFILV
                     SLAMIVGARALWLFDFGDAHTLRKVLSYSGYVWMAFIFMTFAWFVLIDMVRLLFYILD
                     GLLSSR"
     gene            complement(427..>1140)
                     /locus_tag="P5219_12035"
     CDS             complement(427..>1140)
                     /locus_tag="P5219_12035"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HRX27542.1"
                     /translation="RDLGAFTSLLCGVLCGQIYIQAVYAASSDATARKGCLWTSMITP
                     PLGLLGVWIGLALRNSGVAVEASQALPFFIRTYFHPLAAGLLWSGIMITVLGTSVGIT
                     FGVATNLTRDMYLKTKRAAGYDDAKILLASRITVLGTVIVAGLIALAAGKSMILQWAY
                     LGMGIKGAGIIIPLIAAVFLPGRLPSGWSFLSGTCGLAGVFLGAAFFKGMDPLLPGLA
                     LSGLIAFAGLLAGKKATLP"
BASE COUNT          269 a          331 c          361 g          179 t
ORIGIN      
        1 cgtgaggaga ggagcccgtc gagaatatag aagaggagcc tcaccatgtc gatgaggacg
       61 aaccaggcga aggtcatgaa gatgaaggcc atccacacgt atcccgagta ggacagaacc
      121 ttccggagag tgtgggcgtc cccgaagtca aaaagccaca gggcccgtgc gccgacgatc
      181 atggcaagag acaccaggat gaaaagggcg gtccatttcc agccccgtcc tatggcgctt
      241 ctcagcttga agtagacaaa aacgtgcatg cttccgtaaa tcagcatgta gagcgaaaaa
      301 aacacggcaa aactccggct catactccat tcctccctgc acttccggcg gaaaatttag
      361 aggaatatta tagcagtatc ccccgtctcc ccgaatcggc aggagaacgg atcaatccgc
      421 ccatcctcag ggcagggttg cttttttccc cgcaagaaga ccggcgaaag cgatcagtcc
      481 cgagagggcc agccccggca gcaggggatc catgcccttg aagaacgccg cccccaggaa
      541 gacccccgcg aggccgcagg ttccggaaag gaagctccag cctgagggca gccttccggg
      601 caggaacacg gcggcgatga gaggaatgat gatgcccgcc cccttgatgc ccatgccgag
      661 gtaggcccac tggaggatca tgctttttcc cgccgcgagg gcgatcagtc cggcgacgat
      721 aaccgttccg aggacagtta tcctgctggc gaggaggatt ttcgcgtcgt cgtagccggc
      781 agcccgcttc gtcttcaggt acatgtcccg ggtgaggttg gtggccacgc cgaaggtgat
      841 ccccaccgaa gtccccagga cggtgatcat gatccccgac cacaggagcc cggcggcgag
      901 gggatgaaaa taggtgcgga tgaaaaatgg aagggcctgg gatgcttcaa cggccactcc
      961 ggagttccgg agggcgaggc cgatccagac gccgagcagc cccaggggcg gggtgatcat
     1021 ggaagtccag aggcatccct tccgggccgt ggcgtcggag gaggctgcgt agacggcctg
     1081 gatgtagatc tgcccgcaca ggacgccgca gagcagcgat gtgaaggcac cgaggtctct
//
LOCUS       DAOTHD010000864         1139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_151687_length_1139_cov_3.631919, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000864 DAOTHD010000000
VERSION     DAOTHD010000864.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151687_length_1139_cov_3.631919"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /gene="mnmA"
                     /locus_tag="P5219_12040"
     CDS             complement(<1..817)
                     /gene="mnmA"
                     /locus_tag="P5219_12040"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRX27543.1"
                     /translation="MNIRPSTGFSSPSPSFGPRSVVVLMSGGVDSSVTAWLLKKEGRH
                     VAGLTMRIPGQDGLSSGSCCGRDAAAVALSLSIPHYVADLEEEFRESVISPFVESYLA
                     GETPNPCADCNAVLKFGRLWDALEDAFGPVSLATGHYARVLRSGNGCALARGADASKD
                     QSYFLYGIRRDRLPDLFLPLGDRSKEEVRGLAREAGLAVAEKKESMEICFAGEGDYRR
                     LIENRSSRPGLIVDERGAPLGTHGGISNFTVGQRKGLSIASRKGLYVLRIDGEK"
     assembly_gap    888..897
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            959..>1139
                     /locus_tag="P5219_12045"
     CDS             959..>1139
                     /locus_tag="P5219_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00268 family protein"
                     /protein_id="HRX27544.1"
                     /translation="MNTSAEQRTERLTALLRELGKAAVAFSGGADSTLLAAAAVQALG
                     NNAVAVTAASPTLPAW"
BASE COUNT          210 a          360 c          346 g          213 t
ORIGIN      
        1 ccttttcacc gtcgatcctg agcacgtaca gccctttcct ggaagcgatg ctcaggcctt
       61 tcctctgccc cacggtgaag ttggatatcc cgccgtgggt acccagggga gcgcccctct
      121 cgtccacgat gagcccgggc cgggaggaac ggttctctat gagtcgacgg tagtcccctt
      181 cgccggcgaa acagatctcc atgctttcct ttttttccgc cacggcgagt cccgcctccc
      241 gggcgagtcc cctgacctct tccttactcc ggtccccaag gggaagaaaa aggtccggaa
      301 gccggtccct cctgattccg tagagaaagt agctctggtc ttttgatgca tccgctcccc
      361 tggcaagggc acatccgttc cccgaacgga gaaccctggc gtaatgcccc gtggcgaggg
      421 agacgggacc gaaggcatcc tccagggcat cccagaggcg cccgaacttc aggacggcgt
      481 tgcagtcggc gcagggattg ggggtttccc ccgcgagata gctttccacg aagggggaga
      541 tgaccgattc ccggaattcc tcctcaaggt cggccacgta gtggggtatg gatagggaaa
      601 gggccaccgc ggcggcgtcc cttccgcagc aggagccgga tgacaggccg tcctgaccgg
      661 gtattctcat ggtgagcccc gccacatgcc ggccctcttt cttcaggagc cacgctgtga
      721 ctgagctgtc caccccgccg ctcatcagga ccaccaccga ccgggggccg aaggacggcg
      781 aaggggaaga gaagcctgtt gaagggcgta tgttcatttt ttcctccggg gctgattgga
      841 ttgaattcct ttctcgtgta gtatagggaa aaggtggaaa ccctacannn nnnnnnntac
      901 aagggagact ctccgccccc tcgctgaggt cgggcgtcac attcgggagg agaaaacagt
      961 gaacacttcg gcagaacaga gaacggaacg cctgacggcc cttcttcggg aactggggaa
     1021 ggccgccgtc gctttttcgg gaggggcgga cagcacgctc ctcgccgccg ccgccgtaca
     1081 ggccctgggg aacaatgccg tggccgtcac tgccgcctcg cccacccttc cggcatggg
//
LOCUS       DAOTHD010000865         1139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_151757_length_1139_cov_2.488930, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000865 DAOTHD010000000
VERSION     DAOTHD010000865.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151757_length_1139_cov_2.488930"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="P5219_12050"
     CDS             complement(<1..189)
                     /locus_tag="P5219_12050"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRX27545.1"
                     /translation="MIFEEIRKHIEEGERLQALEKFQNLALMGLSLHTDFFKHAAMFG
                     GTSLRIFHGLPRFSEDLDF"
     gene            complement(233..>1139)
                     /locus_tag="P5219_12055"
     CDS             complement(233..>1139)
                     /locus_tag="P5219_12055"
                     /inference="COORDINATES: protein motif:HMM:NF014609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HRX27546.1"
                     /translation="KVPGRTAMKTYPYYKLNAFTIGKSEGNPAAFLDLGTDSLSGDAM
                     LSIAKEHSGFVSEVVYVTRDERGSILLTYYSSECEVAFCGHGTIAAMYEVIRRDDGLR
                     KTPVIEIGTNRKGRLNIYNHINEEDCIYISAPDPLMIGCPLPKQSIGAALGIKEGAIS
                     VSLPLDIIDAGLRTLIVPIKNLADEISMYPDEEGLKQYCLSNDVDIVLVYSMETAAPG
                     YFAHTRVFAPKFGYLEDPATGSGNSAFGCYLLKNGKWNGDSIKIEQGGDNRIFNEVRL
                     RTKDGKVLFGGKAALRIEGKYCLGE"
BASE COUNT          212 a          325 c          321 g          281 t
ORIGIN      
        1 gaaatcaaga tcctctgaaa acctcggcag gccgtggaat attcgaagcg acgtccctcc
       61 aaacatagcc gcatgtttaa aaaagtcagt atgaagtgaa agccccatca gtgccagatt
      121 ctgaaacttt tccagcgcct gtaatcgttc accttcttca atatgctttc ttatttcttc
      181 gaaaatcacc ttcaccttcc tttcaaagag aagtcctttc ccccgcggtc ggtcactcgc
      241 cgaggcaata cttcccttcg attctcaggg cggcctttcc tccgaagagg acctttccgt
      301 ccttcgtcct cagcctgacc tcgttgaaga tccggttgtc gcccccctgc tctattttga
      361 tgctgtcgcc gttccacttt ccgtttttga gcagatagca gccgaaggcg ctgtttcccg
      421 acccggtagc ggggtcctcc aggtagccga atttcggggc gaagaccctg gtgtgggcga
      481 agtaccccgg agctgcggtc tccatggaat agacgagcac tatgtccacg tcgttgctga
      541 ggcagtactg tttcagccct tcctcgtcgg ggtacatgga gatctcgtcc gccaggttct
      601 ttatggggac gatcagcgtc cggagccccg cgtcgatgat atcgaggggc agggaaaccg
      661 atatggcgcc ctcctttatg ccgagggcgg caccaatgga ctgtttcggc agggggcacc
      721 cgatcatgag gggatcgggg gcggagatgt agatgcagtc ctcctcgttg atgtggttgt
      781 agatgttcag cctgcccttc cggttggtgc cgatttcgat gacgggcgtt tttctcagcc
      841 cgtcgtcccg ccggatgacc tcgtacatgg cggcgatggt gccgtggccg cagaaggcga
      901 cttcgcattc cgaggaatag taggtcagga ggatgctccc tctctcgtcc ctggtgacgt
      961 agaccacctc ggagacgaag ccggagtgct ctttggcgat gctgagcatg gcgtccccgg
     1021 agagcgaatc cgttcccagg tcgagaaacg cagccgggtt tccctcggac tttccgatgg
     1081 tgaaggcgtt cagcttgtaa tacgggtatg ttttcatggc cgtcctcccg ggtacttta
//
LOCUS       DAOTHD010000866         1138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_151934_length_1138_cov_3.059095, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000866 DAOTHD010000000
VERSION     DAOTHD010000866.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151934_length_1138_cov_3.059095"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="P5219_12060"
     CDS             <1..365
                     /locus_tag="P5219_12060"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HRX27547.1"
                     /translation="SSASVAYNTARTKGIPAAFCSVFLDHEPSEEFMEKQFQRGLDLA
                     AKRGWVVMIAHTRQGTLDFLKKKSDSPPEKVTFVTMPELMHILWDRSDMSSRQSVSGR
                     NVFKAGRRFRAANEGDFS"
     gene            362..>1138
                     /locus_tag="P5219_12065"
     CDS             362..>1138
                     /locus_tag="P5219_12065"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HRX27548.1"
                     /translation="MKGRVEVIIGAQWGDEGKGRVVDALGSRVDVFARYQGGANAGHT
                     VIVEGKKYVFHLLPSGMLYPCRVCVIGNGVVIDPEQLLNELRELQEQGKDRARLVISG
                     AAHVVMPYHKKLDTAQENQRGKGRKIGTTGRGIGPAYVDKYSRCGVRVEDLLDGDSLR
                     EKLEYNLDEKNLFLSKIYGEEPLPFDEVFDQAVEWGKALAPYVGDASLIIDEAIQEGR
                     GVLFEGAQGTLLDVDHGTYPYVTSSSPVAAGGCVGLGVGPA"
BASE COUNT          252 a          322 c          367 g          197 t
ORIGIN      
        1 catcctccgc ctcggtggcg tataatacag cccggaccaa gggcattccc gccgcattct
       61 gctccgtctt cctcgaccat gaaccgtcgg aggaattcat ggagaaacag ttccagcggg
      121 gactggacct tgccgcgaag agaggatggg tggtcatgat cgcccatacc cggcagggaa
      181 cgctggactt tctgaagaaa aaaagcgact cccctccgga aaaagtgacc ttcgtcacca
      241 tgccggaact tatgcatatt ctgtgggacc ggagcgacat gtcatccagg cagagtgttt
      301 ccgggagaaa cgtattcaaa gcaggccgcc ggttcagggc ggccaatgag ggggattttt
      361 cgtgaaaggt cgggttgagg tcatcatcgg cgcccagtgg ggcgatgagg gaaaaggaag
      421 ggtcgttgac gccctcggat cgagggtgga cgtctttgcc cggtaccagg gaggggccaa
      481 cgcggggcac acggtgatcg tggaggggaa aaaatacgtc ttccacctcc tgccctcggg
      541 aatgctctac ccctgcaggg tatgtgtcat aggcaacggg gtggtcatcg atcccgagca
      601 gcttctcaac gaactgaggg aactccagga gcaggggaag gaccgggcga ggctcgtcat
      661 cagcggcgcc gcccacgtgg tcatgccata ccataaaaag ctcgacacag cccaggaaaa
      721 ccagcggggc aaggggcgga agatcggcac cacgggaaga ggcatcggtc ccgcctacgt
      781 ggacaagtac agccggtgcg gcgttcgggt ggaagacctg cttgacgggg attccctccg
      841 ggaaaagctt gagtacaacc ttgacgagaa aaacctgttc ctctccaaga tctacggcga
      901 ggaacctctt cccttcgatg aagtgttcga tcaggccgtt gaatggggca aggccctcgc
      961 cccctacgtg ggggacgcgt ccctcataat cgatgaagcc atacaggagg gcaggggcgt
     1021 cctcttcgaa ggagcccagg gaacccttct cgacgtggac cacggcacct acccctacgt
     1081 cacgagctcg tcgcccgtgg ccgccggagg atgcgtgggg ctcggcgtgg gacctgcc
//
LOCUS       DAOTHD010000867         1138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_152004_length_1138_cov_2.220683, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000867 DAOTHD010000000
VERSION     DAOTHD010000867.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152004_length_1138_cov_2.220683"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="P5219_12070"
     CDS             complement(<1..812)
                     /locus_tag="P5219_12070"
                     /inference="COORDINATES: protein motif:HMM:NF024601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27549.1"
                     /translation="MNWNETHISHIVADFAEENAFACRALFRVTGIRYTSGIPSLAVT
                     LADRPELLFNREFINSYAETENDIKALLMHEFLHVLLLHTEKFKINTPLLNIALDAIV
                     NAIIHRTYGEAYSGFFARFYPWEGTAKLLRPNPRDFNPLPGDSFDLMHGEIYRGRFSG
                     EDLHELLNFLLPVRLRKDGDLIFIGNHDSERAVISPENGTLLEGILRRMDGTGIWNKP
                     GMRGTGGKLSDESAAVVRFRLDRWKEGVRRILTECLSPDPKRKILSDGEVALL"
     gene            complement(799..>1138)
                     /locus_tag="P5219_12075"
     CDS             complement(799..>1138)
                     /locus_tag="P5219_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27550.1"
                     /translation="IEACKVPQEISDIGEWLLTVASAAGLTGWEEVDRAISLPQEKMI
                     LFPVNKTTSGVGESSGDNSVEFLQRALILARCNRNGYLPDGPVILTCSRLERSIRLMT
                     IDIERFYELE"
BASE COUNT          242 a          298 c          345 g          253 t
ORIGIN      
        1 agcagggcaa cctccccgtc ggagaggatt ttccttttcg gatcgggaga taggcactcc
       61 gtgagaatcc tgcggacacc ctctttccag cggtcgagcc ggaaccggac cactgcagcg
      121 ctttcgtcgc tgagttttcc gcccgtccca cgcatgccgg gtttgttcca gatgccggtg
      181 ccgtccatgc ggcggagaat accctccagg agcgtcccat tttccggaga gatgaccgcc
      241 ctttcagaat catggttacc gatgaagatc agatcgccat ccttccgtag cctgacggga
      301 aggaggaaat tcagcagttc gtgaaggtct tcacccgaaa acctgcccct gtaaatctcc
      361 ccgtgcatca ggtcgaacga atcgcctgga aggggattaa aatcccgagg gttgggacgg
      421 agaagttttg ccgtcccctc ccaggggtaa aagcgggcaa aaaagccgga ataggcctcc
      481 ccgtaagtcc ggtggatgat ggcattgacg atggcgtcga gtgcgatgtt cagcaggggt
      541 gtgttgattt tgaacttctc agtatggagc agcaggacat ggagaaattc gtgcatcaga
      601 agggccttga tgtcgttttc cgtttcggcg taactgttga tgaattcgcg gttgaagagg
      661 agttcggggc ggtcggcgag agtgactgcc agactgggga ttccgctggt gtagcggatg
      721 cccgtcacgc ggaaaagggc ccggcaggca aaggcatttt cctctgcgaa gtcggccacg
      781 atatgactga tatgggtctc attccagttc atagaatctt tcgatatcaa tggtcattaa
      841 tctgatgctc cgttcaaggc gggagcaggt aaggatgacg ggcccatcgg ggaggtatcc
      901 gttgcggttg cagcgggcca ggatcagggc tctttggagg aattcgacgg agttatctcc
      961 ggaagattcc cccaccccgc tggtcgtctt gttcacaggg aaaagaatca ttttctcctg
     1021 cggaagggag atggccctgt cgacctcctc ccacccggtc aggccagcgg ctgaagccac
     1081 cgtgaggagc cattccccga tatcggaaat ttcctgcgga accttgcagg cttcgata
//
LOCUS       DAOTHD010000868         1138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_152049_length_1138_cov_1.890120, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000868 DAOTHD010000000
VERSION     DAOTHD010000868.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152049_length_1138_cov_1.890120"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="P5219_12080"
     CDS             <1..486
                     /locus_tag="P5219_12080"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX27551.1"
                     /translation="AVSASREEEDRKRCIEAGMDGFLPKSSSPGEMAGILSPFLPHKK
                     FHVPEEIAGENPLLAATGGNREAAAEAAEIFLQTVPPLMAEMKRAVAHGDRLLLASHS
                     HRLKGNLVFFGAKKAAGLAQAMEEAAKGGSLEGLPSLLYALISEVNLLMAELRKEWLP
                     A"
     gene            complement(520..>1138)
                     /locus_tag="P5219_12085"
     CDS             complement(520..>1138)
                     /locus_tag="P5219_12085"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HRX27552.1"
                     /translation="SCAAVIGCGGLGGLVGDLMARAGVGRLVFVDGDVFDETNLNRQL
                     LATERLLGKSKAKTAAARAMEINGSVDAEGRQCRFDSFTADGILEGVDIVVDCLDSLS
                     SRRTLFSECAVRGIPLVSGAIAGFWGQVGVVLPGDETLTGYLAGESDTGVEAETGNPP
                     FTPALVAALQCAQAVKLLTGKGTVLTEQLLWIDLADDEFTRLRLR"
BASE COUNT          223 a          386 c          334 g          195 t
ORIGIN      
        1 gccgtatcag cctcccggga agaggaggac aggaaaagat gcattgaagc ggggatggac
       61 ggttttctgc cgaaatcgtc atcccccgga gagatggccg ggatactgtc cccctttctc
      121 ccccacaaaa aattccatgt accagaggaa attgcggggg aaaaccctct gctcgcggcc
      181 acggggggga accgggaggc ggcggcggaa gctgcggaaa ttttcctgca gacggtccct
      241 cccctcatgg cagaaatgaa gcgggctgtc gctcacggtg accggcttct tctggcatcc
      301 cattcccaca ggttaaaggg caatctcgtc tttttcgggg cgaaaaaagc cgccgggctc
      361 gcacaagcca tggaggaagc ggcaaaaggc ggctccctcg aaggccttcc ttccctgctc
      421 tacgccctca tctcggaggt caaccttctg atggccgaat tacggaaaga atggctgccc
      481 gcctgaaggg caggggattc tcctccgggc ctcctccttt cagcgaagcc tgagccgggt
      541 gaattcgtcg tcggcgaggt cgatccacag gagctgttcc gtgagcaccg tccccttccc
      601 cgtgaggagc ttcaccgcct gggcgcattg cagggcggcc acgagagcag gcgtaaacgg
      661 cgggttccct gtttcggcct ccactcccgt atcggactcg ccggcgaggt agccggtgag
      721 ggtctcgtct cccggaagca ccacgcccac ctgtccccag aaacccgcga tggctccgga
      781 caccagggga attccccgga cggcacactc cgaaaagagg gttctccggg acgagaggct
      841 gtcgaggcaa tccaccacga tgtccacccc ctcgagaatc ccgtcggcag taaaggaatc
      901 gaaccggcac tgcctccctt cggcatccac cgatccattg atctccatgg cccgcgcggc
      961 cgccgtcttc gcctttgact tcccgagcag tctctccgtc gccaggagct gccggttcag
     1021 gttcgtttca tcgaatacat cgccgtccac aaacacaagc cggccgaccc cggcacgggc
     1081 catcaggtcc cccacgagcc ccccgaggcc gccgcagccg atgaccgccg cgcaggac
//
LOCUS       DAOTHD010000869         1137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_152291_length_1137_cov_1.616451, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000869 DAOTHD010000000
VERSION     DAOTHD010000869.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152291_length_1137_cov_1.616451"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..331
                     /locus_tag="P5219_12090"
     CDS             161..331
                     /locus_tag="P5219_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27553.1"
                     /translation="MAYRTGAEVSARGYMERLARDKASVVKALLRVWTDRVGQEDLKK
                     EGESILFGLAAF"
     gene            353..>1137
                     /locus_tag="P5219_12095"
     CDS             353..>1137
                     /locus_tag="P5219_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27554.1"
                     /translation="MRENTGHRSLKDYHWNYEYRTSSLKEDNSPVDILHDFYIPALQR
                     SVSYWRVAGYFTSSSLAAASQGFSAFTASGGKMRLITGADLDADDVAAILEGEGTRLT
                     KSLEQELVDSGWPDDVRRGVELLGWMVARGVLEVRVAFRVHGSKGDPLPADSKVDGYV
                     HEKWALFEDAFGNCIRVSGSLNESKTALVRNAENISLDLSWWSFPAPERIQTGKKDFE
                     SLWNNNHPHFRVLSLPEAVRARLISIGRNVKIPVEIDGSSAAVP"
BASE COUNT          241 a          298 c          364 g          234 t
ORIGIN      
        1 gggtgcagga agggccgccg gggcggccgc cgaagaaacg gggtttgccg ccccgctcct
       61 ccggacaggc agcaagcttc ggctttctct tccggaggag cgtgatccga gacggatgaa
      121 atcgccctag acggagtggg atcttctgtg cgggctgatt ttggcgtacc gcacgggggc
      181 ggaggtttca gccaggggct acatggaacg gctggccagg gacaaggctt ccgtcgtgaa
      241 agccctgctt agggtgtgga cggacagggt ggggcaggag gatctgaaaa aagaggggga
      301 aagtattctc ttcggtcttg cagccttttg aaaaaaccct tcggaggatg ccatgagaga
      361 aaatacggga caccggagcc tgaaagacta ccactggaat tacgaataca ggacgtcctc
      421 tttgaaggag gacaattctc ccgtggacat cctccacgac ttttatattc ccgcccttca
      481 gaggtccgtc agctactgga gagtggcggg gtatttcacg tcctcttccc ttgcggctgc
      541 ctcccagggg ttctccgcct tcaccgcctc gggagggaag atgcgcctga tcaccggtgc
      601 ggatctcgac gccgatgatg tggccgccat tctggaagga gaaggcaccc gcctgaccaa
      661 gtccctggag caggaactcg tagattccgg ctggcctgac gatgtccgga gaggggtgga
      721 gctcctcggc tggatggtcg cccggggggt gctggaagtc cgtgtcgcct tcagggttca
      781 tggaagcaaa ggagaccctt tgccggccga ttcaaaagtg gacgggtacg tccatgaaaa
      841 atgggctctt ttcgaggatg ccttcggcaa ctgtatccgt gtatccggtt cgctgaacga
      901 atcgaaaacc gctctggtgc ggaatgcgga aaatatcagc cttgacctgt cctggtggag
      961 ctttccggct cccgaaagga ttcaaaccgg gaaaaaggat ttcgaaagcc tctggaacaa
     1021 caaccatccg cacttcaggg ttctgtcact gccggaagcc gttcgggcac ggttgatttc
     1081 catcggaaga aacgtaaaga ttcctgttga gatcgacggt tcgagcgctg cggttcc
//
LOCUS       DAOTHD010000870         1135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_152610_length_1135_cov_2.241667, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000870 DAOTHD010000000
VERSION     DAOTHD010000870.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152610_length_1135_cov_2.241667"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="P5219_12100"
     CDS             complement(<1..1027)
                     /locus_tag="P5219_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRX27555.1"
                     /translation="MSIEMISVLLFGSMLLLLASGLPVAFVLGGLAVVFTGVFWGPES
                     LFIILARTFSMMSTTTLVAAPLFVLMAVVLERSGVAEDLYEMMYRWSGGIKGGLAVGT
                     VLACTLIAAMSGIASTGVVMMGVMALPAMLKRGYDKRIATGCILAGGVLGPLIPPSIA
                     LVLYGTMAQVSIGALFAGGMAAGMLCSLLIIGYILIRCYLNPKLGPAIPVEERADWDA
                     KISSLKGVILPLLLITAVLGSIYSGIATPTEAAAVGALGAFVCSAIHRRLTWDLIKSV
                     AYTTIQVQGFMMWILFAAQGFAAVYMGLGASRMVVNLVQTYQVGWWTMLIGIQVVWFL
                     LGCVIDAW"
BASE COUNT          254 a          344 c          357 g          180 t
ORIGIN      
        1 tccaggcgtc gatcacgcac ccgagcagga accagactac ctgtatgccg atcagcatgg
       61 tccaccatcc gacctggtag gtctggacca ggttgacgac catcctggag gccccaaggc
      121 ccatgtaaac ggccgcaaac ccctgggcgg cgaacaggat ccacatcatg aacccctgga
      181 cctggatggt ggtgtaggcc acgctcttga tcaggtccca ggtcagacgc ctgtggatgg
      241 cgctgcagac gaaggcgcca agggctccga cggcggccgc ttcagtcgga gtggctatgc
      301 cgctgtatat ggagcccaga actgcggtga taagaagaag ggggaggata actcccttga
      361 gggaggaaat cttcgcgtcc cagtcggccc gttcttccac gggaatggcc ggccccagtt
      421 tcgggttgag atagcacctg atcaggatgt agccgatgat cagcagggag cagagcattc
      481 ccgcggccat gcctccggcg aagagagcgc cgatggatac ctgggccatg gtgccgtaga
      541 gcaccagggc aatgctggga ggaatcaggg gaccaaggac gccgccggcc aggatgcatc
      601 ccgtggcgat ccgcttgtcg taccctctct tcagcatcgc ggggagtgcc atgacgccca
      661 tcatgacgac tccggtggag gcaatgccgg acatggcggc tatcagcgtg caggcaagaa
      721 cggtgccgac cgccagtcct cccttgatgc cgccggacca ccggtacatc atctcgtaga
      781 ggtcttcagc cacgccggaa cgctcaagca ccaccgccat gaggacaaaa aggggcgccg
      841 ccacaagggt cgtggtggac atcatggaaa aggtcctggc caaaatgatg aagagcgatt
      901 cggggcccca gaacacgccg gtgaacacca cggccagccc tccgagaacg aaggcgacgg
      961 gaaggcccga ggcgagcaga aggagcatgc tgccgaaaag gagaacggaa atcatttcta
     1021 tactcatggg ttcacctcag ttcccggcag cggcttcagc atccgcaccg acgacaagct
     1081 cccgcccggt cacggccagg tacgcgtctt ttatggtctt ggtcatcccc tggag
//
LOCUS       DAOTHD010000871         1128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_153907_length_1128_cov_3.161230, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000871 DAOTHD010000000
VERSION     DAOTHD010000871.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153907_length_1128_cov_3.161230"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..490)
                     /locus_tag="P5219_12105"
     CDS             complement(17..490)
                     /locus_tag="P5219_12105"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRX27556.1"
                     /translation="MRSVEKIAASLREKGLKLTPQRRLIIEILSRDRTHPTVDEVYRQ
                     VVVTMPEVSRTTVYNTIREMATLGEVVEVQDISDGGIRYDTCAESHNHFFCLSCRSLI
                     DVDVDLPGAESYSGALEGCQIVKQQLTLYGICPACRDKGLSPPGRGAGKRGLKNF"
     gene            complement(630..>1128)
                     /locus_tag="P5219_12110"
     CDS             complement(630..>1128)
                     /locus_tag="P5219_12110"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRX27557.1"
                     /translation="GGDVGAREARLSIMVGGEEAVFQDMMPLFSLLGKNVVFQGGPGS
                     GQHTKMANQIAIASNMMGVCEALAYSKKAGLDPERVLSSISGGAAGSWSLSNLAPRIL
                     AGDFAPGFFVKHFLKDMKIALDEAERMGLDAPGLKKAFEQYGILASRGFENEGTQALY
                     KLYGE"
BASE COUNT          214 a          355 c          322 g          237 t
ORIGIN      
        1 gactccccgt tctttctcag aaattcttca ggcccctttt ccccgctccc cttcccggcg
       61 gggagagccc cttgtccctg caggccgggc agatgccgta gagcgtgagc tgctgtttta
      121 ctatctggca gccctccagg gccccggagt aggattccgc ccctggaagg tccacatcga
      181 catcgatcag ggagcggcag gaaaggcaga aaaaatgatt gtgggactcg gcgcatgtat
      241 cgtaccggat acccccgtcg ctgatatcct gcacttccac gacctctccc agggtggcca
      301 tctcccgtat ggtgttgtag accgtcgtcc gggaaacttc gggcatggtg accaccacct
      361 ggcggtacac ctcgtccacc gtggggtggg tcctgtccct ggaaaggatc tcgattatca
      421 accgccgctg gggagtgagc ttgagtcctt tctccctcag gctggcagca attttttcca
      481 cggatcgcat aggccatccc tccttgttcc aaaaaactat gttgtaacca ttataaataa
      541 ataaagtttt ccgtcaacag cggattacgg ggtatgtctc cccggaacct gccggaagga
      601 tggcgggggc atccgggacc ggggaacgcc tattcgccgt agagcttgta gagagcctgg
      661 gtgccttcgt tttcaaaacc ccgggatgca agaatgccgt actgttcaaa ggccttcttc
      721 agtccgggcg cgtccagccc catgcgctcc gcctcgtcga gggcgatctt catgtccttc
      781 agaaagtgct tcacgaaaaa gccgggggcg aaatcgcccg caaggatcct gggggcgaga
      841 ttcgagaggg accagctccc cgccgcccct ccggaaatgc tcgacagcac cctttccggg
      901 tcgagaccgg ccttcttcga gtaggccagg gcttcgcaga cccccatcat gttggaggcg
      961 atggcgatct ggttggccat cttcgtgtgc tggccgctgc ccggcccccc ctgaaagacg
     1021 acgttctttc ccagaaggga aaagaggggc atcatgtcct ggaaaacggc ttcttcgccg
     1081 cccaccatga tggacaggcg ggcctcccgt gcgcccacgt cgccgccg
//
LOCUS       DAOTHD010000872         1128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_153909_length_1128_cov_3.052190, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000872 DAOTHD010000000
VERSION     DAOTHD010000872.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153909_length_1128_cov_3.052190"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1128
                     /locus_tag="P5219_12115"
     CDS             <1..>1128
                     /locus_tag="P5219_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRX27558.1"
                     /translation="LPGLTATMGVAVLLPLTFGMESTPALVLLAGIYIGAIYGGSISA
                     ILLKTPGTPAAAATVLDGYELARKGEAARALSVSAIASFTGGMVSTVMLITISPILAN
                     FALRFGAPEYFALAVFGLSIIASISGKSPVKGLLAGMFGLLISTVGLDPVTSFPRFTF
                     GEMNLFNGFAIIPVLIGLFALAEAFVQMEKIFEPSHAIETNFKRGMISLKETIALMPT
                     MIKCALIGTFIGSIPGAGADIAAFVSYNEARRSSKNGSEFGTGCLEGIAAPEAGNNGV
                     TGGAMVPLLTLGVPGDAVTAILLGALIIQGLQPGPLLFTKNADIVYGLFASLFVGNIL
                     MLIIGLLGVRLFCRVVELPKRFIIPVIITLSMVGAYSMNKS"
BASE COUNT          198 a          381 c          300 g          249 t
ORIGIN      
        1 cctgccgggc ctcacagcca ccatgggcgt ggcggtgctc ctgccgctga ctttcggaat
       61 ggaatccact ccggccctgg ttttgctggc cgggatctat atcggcgcaa tctacggcgg
      121 gtccatctca gccattctgc tcaagactcc cggaaccccg gcggcggctg ccacggtcct
      181 cgacgggtac gagcttgcca gaaagggaga ggctgccagg gccttgtccg tttccgccat
      241 cgcctccttc accggcggaa tggtaagcac cgtcatgctc atcaccatat cgcccatcct
      301 cgcgaatttc gccctccggt tcggagcccc cgagtatttt gccctcgcgg ttttcggcct
      361 cagcatcatc gcgagcatct cgggcaaaag cccggtcaag ggactgctgg ccggcatgtt
      421 cggcctgctc atctccaccg tgggccttga tcccgtgacc agtttccccc ggttcacctt
      481 cggcgaaatg aaccttttca acggtttcgc catcattccg gtgctcatcg gtctcttcgc
      541 cctggcggag gccttcgtcc agatggagaa gatcttcgag ccctcccacg ccatcgagac
      601 aaattttaaa aggggcatga tctctctcaa agagaccatt gcccttatgc ccaccatgat
      661 aaagtgcgcc cttatcggaa cctttatcgg gtccatcccc ggcgccggtg ccgatatcgc
      721 ggcattcgtg agctacaacg aagcacgcag gtcatcgaaa aatggaagtg agttcgggac
      781 gggctgcctc gaaggcattg ccgccccgga agcgggaaac aacggcgtca ccggcggcgc
      841 catggtcccc ctgctcactc tcggcgttcc gggggatgcc gtcaccgcca ttctcctcgg
      901 cgctctcatc atccagggac tccagcccgg tccccttctg ttcacgaaga acgccgacat
      961 agtgtacggg ctctttgcct ccctcttcgt gggaaatatc ctcatgctga tcatcggcct
     1021 cctcggcgtc cgcctcttct gccgggtggt ggagcttccc aaaaggttca tcattccggt
     1081 gatcatcacc ctctccatgg tgggtgccta ttccatgaac aagagcat
//
LOCUS       DAOTHD010000873         1126 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_154465_length_1126_cov_1.843137, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000873 DAOTHD010000000
VERSION     DAOTHD010000873.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154465_length_1126_cov_1.843137"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="P5219_12120"
     CDS             complement(<1..515)
                     /locus_tag="P5219_12120"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRX27559.1"
                     /translation="MKALYASDLHGRVNLYEEMIALASSSGVGVVILGGDLFPTVIPN
                     PLALIGGSADFQEDLKGQFRFVEDYLVPAMRAFLEAHPGVRLVFTPGNHDWIPVVKRF
                     GELLPEGLNIHGRTVSFDDVSVFGYACVIDSTFWVKDYARRDLRDDTYVASRFAIVSD
                     TDRLKASPDGE"
     gene            711..>1126
                     /locus_tag="P5219_12125"
     CDS             711..>1126
                     /locus_tag="P5219_12125"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27560.1"
                     /translation="MEGYEPKRVSAEEIQGIVHPGSSMFIESGCAEPQHLVRSLVLDN
                     LFLNDVQVFTTIPLSSYSDFGGEAGDRFRIHSFFVSPGMSGPFSLGKTDHIPLSSDML
                     ERMIREGAIRINTALVQLSPPDADGNMSLGVTVDVMR"
BASE COUNT          208 a          348 c          346 g          224 t
ORIGIN      
        1 tactcgccgt cagggcttgc cttcagcctg tccgtatcgc tcactatggc gaacctgctc
       61 gccacgtacg tgtcgtccct taggtccctg cgtgcatagt ccttgaccca gaaggtggag
      121 tcgatgacgc atgcgtaccc gaacacggac acgtcgtcga aggagacggt ccggccatgg
      181 atgttcaggc cctcgggcag cagctcgccg aaccgcttca ccacggggat ccagtcgtgg
      241 ttgcccggcg tgaacacgag gcgcaccccg gggtgtgcct caagaaaggc gcgcatggcc
      301 ggcacgaggt agtcctccac gaagcggaac tgtcccttga ggtcttcctg gaaatcggcg
      361 ctgccgccga tgagggccag gggattcgga atcacggtgg gaaagaggtc gccgccgagg
      421 atgacgaccc cgaccccgct gctcgaagcc agggcgatca tctcttcgta caggttgacc
      481 cttccgtgca ggtcgctggc gtacagcgct ttcatgggtg tctctctccg tgcggcccat
      541 catatctttc tgggaacccg tgtcaaggaa tctctcccgg gtcgtgaatt gaaagttttt
      601 aatttataaa tggcagagca aatgacgata cgacctccat atcgccttta ccattcgccc
      661 gggggagcgg caggcactgc cgctgggggc ggggaggggg tgtgattgcc atggaagggt
      721 acgagcctaa gagggtttcg gcagaggaaa tccagggaat cgtccatccc ggttccagca
      781 tgttcatcga gtcgggatgc gctgagccgc agcatctggt gcgatcgctc gttctcgaca
      841 acctttttct gaacgacgtc caggtcttca ccaccatacc gctgtcatcc tattccgact
      901 tcggggggga agcgggggac cgcttccgca tccactcgtt cttcgtgtcc cccggcatgt
      961 cggggccctt ctccctggga aagaccgacc acatccccct gtcatcggac atgctcgagc
     1021 ggatgatccg cgagggcgcc atcaggatca acacggcgct cgtccagctc agcccgccgg
     1081 acgccgacgg caacatgagc ctgggcgtca ccgtggacgt gatgcg
//
LOCUS       DAOTHD010000874         1125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_154617_length_1125_cov_2.477570, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000874 DAOTHD010000000
VERSION     DAOTHD010000874.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154617_length_1125_cov_2.477570"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1125)
                     /locus_tag="P5219_12130"
     CDS             complement(<1..>1125)
                     /locus_tag="P5219_12130"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRX27561.1"
                     /translation="DGGCQGAFLAPTEILARQHYLRLAPMIEALGGKCALLTGSLPAR
                     EKAEIQEGLESGEISVAIGTHALLSEKTAFARMGIAIVDEQHRFGVLQKHTFRSKGGS
                     PHVLVMTATPIPRTLTLTVYGDLSVSVISELPPGRKPVATRAISPKKIPGLLSFLEER
                     MEAGERVYWICPLIEESETLDLAAAEARFAELGERFSPFGAGLLHGRLPNGEKENVMD
                     GFQRGDISLLVSTTVVEVGVDVPEATIMVVEDAVRFGLSQLHQLRGRVGRGTGESWCF
                     LICAPSSPEARERIEAFCATSDGFAIAEADLRLRGPGEVCGVRQSGVTDFRVADLIRD
                     RDILETARKEAFGLVAKDPGLLLCPKLEDRLYTVLGRSLN"
BASE COUNT          217 a          346 c          354 g          208 t
ORIGIN      
        1 ggttcagaga ccttcccagc acagtataca gtcgatcctc cagtttcggg cacaaaagga
       61 gcccgggatc cttcgccacg agcccgaagg cttcttttct cgcggtttcg agaatgtccc
      121 tgtccctgat caggtccgcc acgcgaaagt cggtgacgcc ggactgacgt actccgcaga
      181 cttcaccggg cccccggagc cgaagatcgg cctcggcgat ggcaaatccg tcggatgttg
      241 cgcagaacgc ctcgatccgt tccctcgctt ccggcgaaga aggggcgcat atgagaaagc
      301 accaggattc gccggttccc cgcccaaccc ggccccgaag ctggtggagc tgggacaggc
      361 cgaaccgcac ggcatcctca accaccatga tggtggcctc gggcacatcc actcctacct
      421 ccactacggt agtggagacg aggagagaaa tgtccccccg ctggaagccg tccatgacat
      481 tttccttctc gccgttggga aggcggccgt ggagcagacc ggccccgaaa ggggaaaacc
      541 gctcgccgag ttcggcgaac cgggcctccg ccgccgccag gtcaagggtt tcgctttcct
      601 cgatgagggg gcagatccag tagacccgtt ccccggcttc catccgttcc tcgaggaacg
      661 agaggagacc cggaattttc ttcggcgaaa ttgccctggt cgcaacgggt tttcttcccg
      721 ggggcagctc gctgataaca gagacggaaa ggtcgccgta gaccgtgagg gtaagggtgc
      781 ggggaatggg ggtagccgtc atgaccagga catgggggct cccgcccttt gaccggaagg
      841 tgtgcttctg gaggacgccg aaacggtgct gctcgtccac gatggcaatg cccatcctgg
      901 cgaaggccgt tttttcggag agcagggcat gggttccgat ggccacggaa atctcccctg
      961 attcaagtcc ttcctgtatt tcggctttct ccctggccgg gagggaacct gtgagaaggg
     1021 cacattttcc gccgagggct tcgatcatgg gggcgagacg gaggtagtgc tggcgcgcga
     1081 gaatctccgt gggagcgaga aatgcgccct ggcaccctcc atcca
//
LOCUS       DAOTHD010000875         1124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_154761_length_1124_cov_4.424696, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000875 DAOTHD010000000
VERSION     DAOTHD010000875.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154761_length_1124_cov_4.424696"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1124)
                     /locus_tag="P5219_12135"
     CDS             complement(<1..>1124)
                     /locus_tag="P5219_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRX27562.1"
                     /translation="DEHIVHQVLLETKKRLHFLTDVGVGYLSLIRRADTLSGGESQRI
                     RLATQIGSKLSGVLYVLDEPTIGLHSRDTEKLVRTLESIRDLGNTVVVVEHDRETMMA
                     ADSIIEMGPGAGDGGGNIVFAGEYEEAFKSPFLTGPYLRGECNGIVRPKERRRPKGTL
                     TVKGARHNNLKGINVEFPTGLFITVTGVSGSGKSSLIHDVLYKGMRRHLDRDFRERAG
                     NHKSIVGWENFRNIILVDQSPIGRTPRSNPATYTGLFTLIRELFAELPEAKLRGYLPG
                     RFSFNVRGGRCEACGGGGSTKVSMLFLPDVYVPCEVCGGKRYNRETLEVKFKGKSISD
                     VLDLTVDEALQFFRDIPRIASKLTLIAEAGLGYIRLGQSA"
BASE COUNT          218 a          331 c          293 g          282 t
ORIGIN      
        1 tgccgattgg cccagtcgta tataccccag ccccgcctct gcgatgagag tgagcttgga
       61 ggcgattctc gggatgtccc tgaagaactg cagggcctcg tcaacagtca ggtccagaac
      121 gtcggatatg gattttccct tgaacttgac ttcgagggtt tccctattgt accgtttgcc
      181 tccgcagact tcgcagggca cgtagacatc gggcagaaac agcatggaaa ccttggtgga
      241 cccgccgccg ccgcatgctt cgcaccgccc tcccctcacg ttgaagctga acctgccggg
      301 aagatatccc ctcagtttcg cttccgggag ttcggcgaaa agttcccgga tgagggtgaa
      361 caatcccgtg tatgtggctg gattcgacct cggagttctc ccgatggggc tttggtcgac
      421 gagaatgatg tttctgaaat tttcccaccc gacaatgctt ttatggtttc ccgcccgttc
      481 ccggaaatcc cggtccaggt gccgtctcat ccccttgtaa agtacgtcat gaataaggct
      541 gctcttcccc gatcctgaaa cgcctgtcac ggtgatgaag agccccgtcg gaaactccac
      601 attgatgcct ttcagattgt tgtgccgggc tccctttaca gtaagggttc ctttgggtct
      661 ccgtctttcc ttcggccgga cgatgccgtt gcattccccc cgcaggtagg ggccggtaag
      721 aaacggtgat ttgaacgcct cttcgtactc tccggcaaaa acgatatttc cccctccgtc
      781 acctgccccc ggtcccattt ctatgatgga gtcggctgcc atcatggttt ccctgtcgtg
      841 ttcaaccact acgacggtgt taccgaggtc gcggatcgat tcgagggtcc tgacgagttt
      901 ctcggtatcc cgtgaatgaa ggcctatggt aggttcatcc agcacgtaca atacaccgct
      961 cagtttcgat ccgatctggg tggcgagccg gattctctgg ctttctccac cgctgagggt
     1021 atcggctctc cgaataagag aaaggtatcc cacccccacg tcagtgagaa aatgaaggcg
     1081 ttttttcgtc tccagaagaa cctggtgtac gatatgctca tccc
//
LOCUS       DAOTHD010000876         1123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_154981_length_1123_cov_4.104869, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000876 DAOTHD010000000
VERSION     DAOTHD010000876.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154981_length_1123_cov_4.104869"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..>1123
                     /locus_tag="P5219_12140"
     CDS             24..>1123
                     /locus_tag="P5219_12140"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HRX27563.1"
                     /translation="MDRVVKGEMTAAEGAARLGMSRRQIFRLKAKILTSGISSLSHGN
                     RNRRPPNALSEETRSMVCGKAAGEYRGASLNHMSELLDRIDGVRISAKTIGRILKDAG
                     VANPHGRRGRKSHRSRERRERFGSLVQVDATPFEWLEDGNKWSLHGAIDDATGTVLSL
                     RFEKNECSSGYLRVMEDMLGTWGVPAALYTDRHTIFFSPKKGEELSEEDIIEGRKVPL
                     THYGKMLDMLGIEHIPALSPQAKGRVERLWGTLQHRLLVEMRVAGVRTMEEANDFLPA
                     YMELHNGLFTVEPANGTTDFLPCPEQERLRLILGHRDERKASAGSEISWQGKKYQLAD
                     EKGEIVLLRRGETVTAIRTSDGGLYALREGEGG"
BASE COUNT          258 a          309 c          380 g          176 t
ORIGIN      
        1 aagaagcgac gagatcggcg gtcctggaca gggtcgtgaa aggagagatg actgctgcgg
       61 aaggtgcggc ccgcctcggc atgagcagga ggcagatctt ccgcctgaaa gcgaagatcc
      121 tgacgtcggg catttcttcc ctgtcccacg gaaaccgcaa caggagacct ccgaatgccc
      181 tttcggagga gacgaggtcc atggtgtgcg gcaaagctgc cggggaatac agaggggcga
      241 gcctaaacca catgtccgag ctcctggaca gaatcgatgg agtacggata agcgccaaga
      301 ccatagggcg gatcctcaag gacgcgggag tggcgaaccc ccacggaagg agggggagga
      361 agagccaccg gagccgggag cgccgcgaac gtttcgggag tctggtccag gtggatgcca
      421 ctcccttcga gtggctggag gacgggaaca aatggtcgct ccacggggcc atagacgacg
      481 ccaccggcac ggtactgtcc ctgagattcg agaagaacga atgttcttca ggctatctcc
      541 gcgtcatgga ggacatgctg ggtacctggg gcgttcctgc ggcactctac acggaccgcc
      601 acaccatctt cttctccccg aagaagggag aggagctgag tgaggaggac atcatcgagg
      661 gaaggaaagt ccccctcacc cattacggga agatgctcga catgctgggc atagaacata
      721 tcccggccct cagtccccag gcgaagggcc gggtggagcg cctgtggggg acgctgcagc
      781 accgcctgct ggtggaaatg cgtgtagccg gtgtcaggac gatggaagaa gccaacgact
      841 ttctccccgc atacatggaa ctccacaacg ggcttttcac cgtggagccg gcgaacggga
      901 cgacggattt tctcccctgt cctgaacagg aaagactgcg gctcatcctg ggacacaggg
      961 acgagcgcaa ggcttccgcc gggtccgaga tatcctggca gggcaaaaag taccagctgg
     1021 cggacgaaaa gggagagatt gtccttctcc gcaggggtga gacggtcacg gcgatccgca
     1081 cctccgacgg tggtctctat gctctgcggg aaggggaagg agg
//
LOCUS       DAOTHD010000877         1123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_155028_length_1123_cov_2.882022, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000877 DAOTHD010000000
VERSION     DAOTHD010000877.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155028_length_1123_cov_2.882022"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="P5219_12145"
     CDS             <1..382
                     /locus_tag="P5219_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27564.1"
                     /translation="DFPMTFFGDDEYAPMSATRYYTGFWQAKMNFGALDADKIRKIWT
                     SHSYITKKGELVEKITNGKEWIPANLASNTICSHDGGYPESYIGSTTDTKIATVDFNQ
                     VRYSVGRPCEYEGMPRIFDLHPYK"
     gene            535..>1123
                     /locus_tag="P5219_12150"
     CDS             535..>1123
                     /locus_tag="P5219_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27565.1"
                     /translation="MKPGPSGLDQFLLTTAPLRKRAAEEGRDAFLALLPEEGIPGAGD
                     TPRAAVRVENRVLEIVQPGFARALGLEDEGKAALFTLRLQEHLPPFAASAPAVDRSTP
                     PLRTGLAAAAGALIGTGLFSPLTALFFGSREAGFLLGAPLGAFALSLAALRLPENRKL
                     LWALAALLGLAGLREALAFLGSGSLFSRGWNLLTKN"
BASE COUNT          216 a          386 c          312 g          209 t
ORIGIN      
        1 cgacttcccc atgaccttct tcggcgacga cgagtacgca cccatgagcg caacccgcta
       61 ctacaccggg ttctggcagg ccaaaatgaa cttcggcgcg ctcgacgccg acaagatcag
      121 aaagatctgg acctcccact cctacatcac gaagaagggc gagctggtgg aaaagatcac
      181 caacggcaag gaatggattc ctgccaacct cgccagcaac accatctgca gccatgacgg
      241 gggatacccg gaaagctaca tcggttccac caccgacacc aagatcgcga cggtggactt
      301 caaccaggtc cggtattccg tcggcagacc ctgcgagtac gagggaatgc ccaggatttt
      361 cgatctccac ccgtacaagt agactagtgc cgtactccag gaagcggacc cttcggggtc
      421 cgcttctttt ttcaggaata ccccgccccg tttttcctga aaaatcccct tgcccttccg
      481 ccccggggag gatacgctag tgtccattca tgaagatcca aaggaggcga tcccgtgaaa
      541 ccgggaccat caggccttga ccagttttta ctgacaacgg caccccttcg gaaaagggct
      601 gcggaagaag gcagggacgc cttcctggcc ctccttcccg aagagggaat tcccggggcc
      661 ggggacaccc ccagggctgc cgtccgtgtg gaaaaccggg ttctggagat tgtccagccc
      721 gggttcgccc gggccctcgg cctggaggac gaaggaaagg cagcgctctt cacgctgaga
      781 ctgcaggagc acctgccacc ctttgcggcg tccgccccgg ccgtcgaccg gagcactcct
      841 cctctccgga cgggacttgc cgcagcagcg ggggccctta tcggcacggg tcttttctct
      901 cccctcacag ccctcttttt cggctcccgt gaagcgggat ttctcctggg cgcgcccctg
      961 ggggcctttg ccctttccct ggccgccctc cgccttcccg aaaaccggaa gctgctgtgg
     1021 gctctggcgg ccctcctcgg cctggccggc ctcagggaag ccctcgcctt cctcgggtcc
     1081 ggatctcttt tttccagggg ctggaacctc ctcacgaaga aca
//
LOCUS       DAOTHD010000878         1123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_155104_length_1123_cov_2.161049, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000878 DAOTHD010000000
VERSION     DAOTHD010000878.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155104_length_1123_cov_2.161049"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..>1123
                     /locus_tag="P5219_12155"
     CDS             97..>1123
                     /locus_tag="P5219_12155"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRX27566.1"
                     /translation="MRIEKIRFRNLNSLVGEWEIDLTHPAFASDGIFAITGPTGSGKT
                     TILDAVCLALYGRTPRLNRVTKSGNEIMSRQTGECFAEVTFRTGAGCFRCHWSQRRAR
                     KKPDGELQAPKHEIADGETGTLFESKIRGVAGQIEEATGMDFDRFTRSMLLAQGGFAA
                     FLQAAPDERAPILEQITGTEIYSHISMEVHERRREERERMNLLQAETAGITVLQPEEE
                     EALRRDLAEKAREEEAAAGKLENTAQAMAWLTALDGLGKEISGLGEEETELQGEWAAF
                     EPDLERLHRALRAASLEGAHATLEALRNQQANDSEALKAEEESLPELESRAREGAQAL
                     LEAELVTI"
BASE COUNT          227 a          352 c          397 g          147 t
ORIGIN      
        1 ttcccgaaga ccagcatgcc gagctgttcg cggtctaccg ggaagcggtc ctctccctct
       61 tggagcgcgg ggggcagccg gaacaggggg gccgccatga ggattgaaaa gatacggttt
      121 cgaaacctca actccctggt gggggaatgg gagatcgacc tgacccaccc cgccttcgcg
      181 tccgacggca tcttcgccat cacggggccc accggctccg gaaagaccac catcctggac
      241 gccgtctgtt tggccctcta cggccggacc ccccggctga accgggtgac gaaaagcggc
      301 aacgagatca tgtcccgcca gacgggggag tgctttgccg aagtgacctt ccggaccgga
      361 gcggggtgct tccggtgcca ctggagccag cgccgggcac ggaaaaagcc ggacggcgaa
      421 ctccaggccc cgaagcacga aatcgccgac ggcgagacgg gcaccctctt cgagtccaag
      481 atccggggtg tggccgggca gatcgaggaa gccaccggaa tggatttcga ccggttcacc
      541 cggtccatgc tcctggccca ggggggcttc gccgccttcc tgcaggcggc cccggacgaa
      601 agggccccca tcctggagca gatcacgggc acggagattt acagccacat ctccatggag
      661 gtccacgaac gccggcggga ggagcgggag aggatgaacc tgcttcaggc ggagacggcg
      721 gggatcacgg tcctgcagcc ggaggaggag gaagccctcc ggcgggacct ggcggagaag
      781 gcgcgggagg aagaggcggc cgccggaaag ctggagaata cggcacaggc catggcctgg
      841 cttacggccc tggacgggct gggaaaagag atttccggcc tgggggagga agagactgag
      901 ctgcagggtg aatgggccgc ctttgaaccg gacctggaaa gactccaccg ggctctgagg
      961 gcggcgtccc tcgagggcgc ccatgccacc ctcgaagccc tgcggaacca gcaggccaac
     1021 gattcggaag ccctgaaagc cgaagaggaa agcctccccg aacttgagtc ccgggcccgg
     1081 gagggagccc aggctctgct cgaagcggag ctggtgacca tcc
//
LOCUS       DAOTHD010000879         1119 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_155885_length_1119_cov_3.569549, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000879 DAOTHD010000000
VERSION     DAOTHD010000879.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155885_length_1119_cov_3.569549"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="P5219_12160"
     CDS             complement(<1..408)
                     /locus_tag="P5219_12160"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRX27567.1"
                     /translation="MNRMRTILLWSLCFLVIAAAGAVSAAEKGGVLRVAVIDEPPTLD
                     QHVVTSDLTTMIAQHIFEGLYTFDSKYSPVPLLVKSEEMKDDGKTAVLTLREGVKFHN
                     GKELDAEDVLASMERWGKFGARGPVLFSNLEKVE"
     gene            complement(509..>1119)
                     /locus_tag="P5219_12165"
     CDS             complement(509..>1119)
                     /locus_tag="P5219_12165"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRX27568.1"
                     /translation="WRLVTEKLRKGDISTHCYRGPVPVVDGNGTVYPYLFEARERGVL
                     FDLGHGGGSFLFRNAVPAFAQGFYPDSISSDLHVQSMNGAMMDMPTTMSKCLAMGMPL
                     SDVIARSTAVPARMIGHPELGHLSPGAPGDVTLWNLREGTFGFKDSVGGRLRSDRRFE
                     CEMTILAGEVVWDLNARDGTAYGEIPFGEGIREGEFFIPPER"
BASE COUNT          229 a          362 c          306 g          222 t
ORIGIN      
        1 ttccaccttt tccagattgc tgaagagcac gggaccgcgg gcgccgaact tgccccagcg
       61 ctccatggag gcgagcacgt cttccgcatc cagctccttg ccgttatgga acttcacgcc
      121 ttcgcggagc gtaagaacgg ctgtttttcc atcgtccttc atttcttcgg atttcaccag
      181 gagcggaacg ggactgtact tggaatcgaa agtatagagc ccctcgaaaa tatgctgggc
      241 gatcatggtg gtcaggtcgg aagtcaccac gtgctggtcg agggtgggag gttcgtcgat
      301 gacggcgaca cggaggaccc cgcccttctc cgctgcagaa accgctccgg cggcggcgat
      361 gacaaggaaa cacaggctcc acaacaggat agttctcatc cgattcatat caatcactcc
      421 ttttcagatc tctcaatcga attacgcccc aaatactttc aacatccctt ttgcccgtac
      481 caccccctcc cccgctgccg gaaaaaactc aacgctccgg aggaataaaa aactcgcctt
      541 cccgaatgcc ttcaccgaag ggaatctcgc cgtatgccgt cccgtcccgg gcgttgaggt
      601 cccagaccac ttcccctgcg aggatcgtca tttcgcactc gaacctccgg tcagaccgaa
      661 ggcgcccccc aacggaatcc ttgaagccga aggtcccctc ccggaggttc cagagcgtca
      721 cgtctcccgg cgctccgggc gaaaggtgcc ccagctccgg atggccgatc atccgtgccg
      781 gaaccgccgt tgacctggcg atgacatcgc tgagaggcat ccccatggcg agacacttgg
      841 acatggtggt gggcatgtcc atcatggcgc cgttcatgct ctggacgtga aggtccgagg
      901 agatggaatc ggggtagaat ccctgggcaa aggcaggcac ggcgttccgg aaaaggaagc
      961 ttcccccgcc gtggcccagg tcgaacagga cacctctttc ccgggcttcg aagaggtacg
     1021 ggtacaccgt tccgtttccg tccaccacgg gaacgggccc cctgtagcag tgggtgctga
     1081 tgtcgccctt cctcagcttt tccgtcacaa gccgccagt
//
LOCUS       DAOTHD010000880         1119 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_155929_length_1119_cov_2.584586, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000880 DAOTHD010000000
VERSION     DAOTHD010000880.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155929_length_1119_cov_2.584586"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1119
                     /locus_tag="P5219_12170"
     CDS             <1..>1119
                     /locus_tag="P5219_12170"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRX27569.1"
                     /translation="TSSREKEGPYETRLFTNIEGIEPAPPVPVPPKGYTRDEAVREGG
                     RCLLCECMECVKSCAFLQHYKGYPKKFAREIYNNLSVIQGTRLANGMINSCTLCGQCE
                     RVCPGGFSMRDLCRTARQEMVLQQKMPPSAHEFALEDMAFSNSQGASLLRHDPGHSGS
                     AFLFFPGCRLAGISPRTTESVYGHLRNVLDGGVGFWLRCCGAPARWAGRRKELEAAGK
                     ELLDQWRSMGSPLVIAACTSCLEVFRKELPEVRAVSLWTVLQEKGVPETVPIPSATAA
                     LHDPCTAAELPEVRAAVRDLVARTGRPAEELPLSGELASCCGFGGLQECANPGLAAKT
                     ARERCGQSSSDFLVYCAMCRDLFARTGKRTVHLLDLFFP"
BASE COUNT          183 a          354 c          367 g          215 t
ORIGIN      
        1 cacgtcctcc cgggagaagg aggggcccta tgagaccagg cttttcacga atatcgaagg
       61 gattgaaccc gcgcctcccg tgccggttcc gcccaagggg tatacccggg acgaggctgt
      121 ccgggagggc gggcggtgcc tgctctgcga gtgcatggaa tgcgtgaagt cctgtgcctt
      181 cctgcagcac tacaaaggat acccgaaaaa gttcgcccgt gagatctaca ataatctctc
      241 ggtcatccag ggaacaaggc tcgccaacgg catgatcaac tcctgtaccc tgtgcggcca
      301 gtgcgaacgg gtctgccccg gcggtttttc catgagggac ctgtgtcgta cggcccggca
      361 ggagatggtg ctccagcaga agatgccgcc ctcggcccac gagttcgccc ttgaggacat
      421 ggccttttcc aactctcagg gggcgtctct tctgcgccat gatcccggcc acagcgggag
      481 cgcctttctc ttttttccgg ggtgccgcct tgcggggatc tctccccgga caacggaatc
      541 cgtctacgga catctccgga atgttcttga cggcggagtg gggttctggc tccggtgctg
      601 cggagctccg gcacgatggg ccggccgccg gaaggaactg gaggcggcag ggaaagagct
      661 gcttgaccag tggcgctcca tgggctctcc gctggtgatc gccgcctgca cgagctgcct
      721 ggaggttttc cggaaggaac tgcccgaagt ccgggccgtc tctctttgga ctgtcctgca
      781 ggaaaagggc gtcccggaga ctgtcccgat cccttcggcg accgccgcgc tccacgaccc
      841 ctgtacggct gcggaactgc ctgaggtgcg ggccgcggtg cgggacctgg tggcccgtac
      901 agggaggccg gcggaggagc tgcccctgtc gggagagctc gcgtcctgct gcggtttcgg
      961 cggcctccag gagtgcgcca accccggcct cgcggcgaaa acggcacggg aacggtgcgg
     1021 acaatcttcc tcagatttcc tcgtctactg tgccatgtgc cgggacctct tcgcccggac
     1081 gggaaagaga acggtccacc ttcttgacct gttctttcc
//
LOCUS       DAOTHD010000881         1118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_156157_length_1118_cov_2.380997, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000881 DAOTHD010000000
VERSION     DAOTHD010000881.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156157_length_1118_cov_2.380997"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..536)
                     /locus_tag="P5219_12175"
     CDS             complement(114..536)
                     /locus_tag="P5219_12175"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRX27570.1"
                     /translation="MEKMPRSRGCFVCGSPEENRRSLGVEIFWNEEENRTEIRIHPDP
                     TWCGYEGIVHGGIIASVFDDAMAWAVRKTIGSWAMTGEMSVRYLRPVQEGKEYVVEGR
                     VDRMSGRKITTAARMADGSGKTVAEATALFIRMPGGGS"
     gene            635..910
                     /locus_tag="P5219_12180"
     CDS             635..910
                     /locus_tag="P5219_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27571.1"
                     /translation="MRIAGMREDDDGTCLYLVEGEGPSGERLLLLYDENGGKARPAEP
                     AGAEALFREGLLERCSFPAEEVFFPDELEDLERKLLSAMKKEEDEEQ"
     gene            907..>1118
                     /locus_tag="P5219_12185"
     CDS             907..>1118
                     /locus_tag="P5219_12185"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRX27572.1"
                     /translation="MSRTVFFNGKIYTPRGREEALLVDGQTIAAVGRSGDVLERAEGA
                     ERIDLEGRLMLPGFIDGHMHFLAYALS"
BASE COUNT          202 a          332 c          350 g          234 t
ORIGIN      
        1 gagctgtaga gtcgcagttt tgcctcggcg agttcagggt cgataaacga tacgagattc
       61 atgggtcttc gtcagcctcc ttgggcacta ttcggttccc ctgtttccgg gggtcagctt
      121 ccgccgccgg gcatcctgat gaagagggcg gtcgcctcgg ccaccgtttt tccgctgccg
      181 tcagccatcc gggccgccgt ggtgattttc cggcctgaca tcctgtccac ccggccttcc
      241 accacgtatt cttttccttc ctgcacgggg cggagatacc ggacggacat ttcgcccgtc
      301 atcgcccacg agccgatggt cttcctcacg gcccacgcca tggcgtcatc gaacaccgag
      361 gcgataatgc ccccgtggac gatcccctcg tagccgcacc acgtgggatc ggggtgtatc
      421 ctgatttccg tcctgttttc ctcttcgttc cagaatatct caacgccgag gctccgccgg
      481 ttttcctccg gggagccgca gacgaagcat cctcttgacc ggggcatttt ttccatggaa
      541 tcccgctcct ttcgggaagt tgctggtcca attatatccg aagggtccgc ggacggtata
      601 atgaaatcca ccatactgcc cgggagggat aggcatgcgc atcgcaggca tgagggaaga
      661 cgacgacgga acctgcctct acctggtcga gggggaaggc ccctcggggg agaggctgct
      721 tttgctttac gacgagaacg gcgggaaagc ccgtccggcg gaacccgccg gggcggaggc
      781 ccttttccgg gaaggactcc tggagcggtg ttccttcccg gcggaggagg ttttcttccc
      841 cgatgagctg gaggacctgg aaaggaagct cctttccgcg atgaagaaag aggaggatga
      901 agagcagtga gcagaaccgt ttttttcaac ggaaaaatat acacgccccg aggcagggag
      961 gaagcactcc ttgtggacgg gcagaccatc gcggccgtcg gccggagcgg ggatgtgctc
     1021 gaaagggcgg aaggggcgga gcggatcgac ctggaggggc gtctcatgct cccggggttt
     1081 atcgatgggc acatgcactt cctcgcctat gccctttc
//
LOCUS       DAOTHD010000882         1117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_156328_length_1117_cov_2.907721, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000882 DAOTHD010000000
VERSION     DAOTHD010000882.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156328_length_1117_cov_2.907721"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..903)
                     /locus_tag="P5219_12190"
     CDS             complement(196..903)
                     /locus_tag="P5219_12190"
                     /inference="COORDINATES: protein motif:HMM:NF019947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRX27573.1"
                     /translation="MKTEGQTSGNREFLEKYIPLLDFFAEVCGSEYELVLHDVSRPES
                     SIIAIRNGQISGRSVGSTMSDYAPTFVKLIRSGDCSEDMVAHMDRTKDNRILESHTFF
                     IKDDRGELRGMICANHDVTDLIRLHDTLHEKIRMLNGIAGRSAIPGPEEEPAPLDDLF
                     PAGKESNLDGLMDVLIEKAASEFSAPPGAMTPEERTRFVGVLKGRHLFSMKGAVPKVA
                     RRLGVSEATVYRYLKKA"
BASE COUNT          229 a          323 c          286 g          279 t
ORIGIN      
        1 tcccaggcgc cccgggctac ggcttcattg ccggtcagta ttaccttcat tgacaggtcg
       61 ctcctttcag atttctcttc gcagagcaag gtgataataa atttttatca cactacgccc
      121 tgatccgcaa caaaaaaaga gactccctcc ggaatctctt ttcgctttcg gggaacaggt
      181 gcggtccggg tatgatcacg ctttcttcag atacctgtac acggtcgcct cggaaacccc
      241 gaggcgacgg gcgactttcg gcaccgctcc cttcatggag aagagatgtc tccctttcag
      301 aacccctacg aacctggttc tctcttccgg ggtcatcgcc ccgggaggtg cggaaaattc
      361 cgaggccgcc ttctctataa gcacgtccat aaggccgtca aggttcgatt cctttccggc
      421 tggaaacagg tcgtccagcg gagcgggctc ttcttccggt cccggtatcg cagacctgcc
      481 tgcaattccg ttcagcatgc ggatcttctc gtgaagggtg tcatgaaggc gaatcaggtc
      541 tgtgacgtca tggttcgcgc atatcatccc ccgaagctct ccccggtcat ccttgatgaa
      601 aaaggtgtgg gattccagga tacggttgtc ctttgtcctg tccatgtggg cgaccatatc
      661 ctcgctgcag tctccgctcc ggatcagctt gacgaatgtc ggtgcataat cggacatggt
      721 gctccccacg gaacggccgc tgatctgccc gttccgtatg gcgataatgg acgattccgg
      781 cctggacaca tcgtgaagca ccagttcgta ttcagatccg cagacctcgg cgaaaaagtc
      841 gagaagcgga atgtattttt ccagaaattc ccggtttccg gatgtctgac cttcggtttt
      901 catgctgtgt catcccttca ggaagaactc tttcattggc tgataaaaaa ttcatatcat
      961 ttttcaggcc tgatgtaaag gctgagaaat cgcctgtgcc ttccagcccc cgggaaagac
     1021 cagaaaaaaa cggcggttcc ggggggaatc cgccgcctgc tccctgaaac gtccttattc
     1081 ctgggggtga gctcccggtc atttccctgt ccggatg
//
LOCUS       DAOTHD010000883         1115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_156768_length_1115_cov_3.011321, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000883 DAOTHD010000000
VERSION     DAOTHD010000883.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156768_length_1115_cov_3.011321"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            521..>1115
                     /locus_tag="P5219_12195"
     CDS             521..>1115
                     /locus_tag="P5219_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HRX27574.1"
                     /translation="MSENNNTPGLGLSFLVLVLSAAIISYGVLSLGVDAHIPIIFSAV
                     LSCLVGVFVIKKPWGEIEEGGLNGIAIALQAIVILMIIGMVIGIWVQSGVVPTLIFYG
                     LKILSPGMFLLACLLITSIISLSTGSSWTTSATIGVALMGVGFGLGIPAPLSAGIIVS
                     GAYFGDKMSPLSDTTNLAPAVAGANLFDHIKAMIWSTG"
BASE COUNT          264 a          287 c          237 g          327 t
ORIGIN      
        1 gtcaacctcc cggatctggc tctcctttta ggaacggctt tgctccttgt ctctttttgg
       61 aaaacttccc aggaataaaa acaaccacat gctttacgca aacacttcga aatgaaaatt
      121 tacggagcgt cctttctcgc tttagccagc taaataattt ttataataaa aaattttttt
      181 tcgcctgcgc attacgtcaa aattgcgaaa attgccttca caagggaacc tccttttatt
      241 tttgcttcct ctatatgccg taaaaaaaga ccctctcgaa acttttttca ggataaagaa
      301 gacgagaaaa tttttctttc agccttccgg aagcgtgaat attttccgta ttctgataca
      361 atgtccctat ctccctttgg agggtctcaa aggtaatcat ttctctctcc tttatggagt
      421 ttaaattttt gtgtctcggg aggtgaaaac actagaaaaa aaaaggtttc atggaaacag
      481 ggaaaaaagt agaacaaaat cgttaaggag ggatacgcgt atgtcggaaa ataataatac
      541 ccccggattg ggactttcct ttctcgtgtt ggttctgtcc gcagcaatta tcagctacgg
      601 cgtgctttca ctgggagtgg atgcccacat tcccattatc ttttctgcag ttctttcatg
      661 tcttgtaggg gtttttgtca tcaaaaaacc ctggggagaa attgaagagg gaggcctgaa
      721 cggtatcgcc atcgccctgc aggccatcgt catcctgatg atcatcggca tggttatcgg
      781 catctgggtc cagagcggtg tagttcccac gcttatcttc tacggcctga aaattctttc
      841 tccggggatg ttccttctcg cctgccttct cattacatcc atcatttcgc tttccacggg
      901 atcgtcctgg accacctccg ccaccatcgg tgtggctctt atgggcgtag gcttcggtct
      961 cggcattccc gctcctctga gcgccggtat catcgtgtcg ggagcctact tcggggataa
     1021 aatgtccccc ctgtcggaca ccaccaacct cgcccccgcc gtagcgggcg ccaacctttt
     1081 cgatcacatc aaggccatga tctggtccac gggcc
//
LOCUS       DAOTHD010000884         1115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_156771_length_1115_cov_2.980189, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000884 DAOTHD010000000
VERSION     DAOTHD010000884.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156771_length_1115_cov_2.980189"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1115)
                     /locus_tag="P5219_12200"
     CDS             complement(<1..>1115)
                     /locus_tag="P5219_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_except=(pos:complement(1070..1072),aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRX27575.1"
                     /translation="EVMGTNNVDHCAHLUHSATVAGLGETLGSGAMTNDFESIKSADT
                     ILIIGSNTTENHPVIGSFIKEQKRKGKCTLIVCDPRKIEMAKYADVYIRQKSGSDVAL
                     LNGLMHVIIRENLHAAEFVEKNVNNFDKLRETVARYTPEYVSGITGVAPELIEDAARA
                     YARGPNSAIFYTMGITQHVTGTNNVRTVANLALLCGMLGRPGTGVNPLRGQNNVQGAC
                     DMGCLPDVIPGYQKVALPAARQKAKDVWGADIPETPGLSVVEMMEAAGEGKIRGLYIM
                     GENSMVTEPDSGHVRHALKNLEFLVVQDIFLTETAELAHVVLPASSWGEKDGTFTNTS
                     RAVQRVRKAVDSPGEARPDWEILLAVGKRMGAPWDDM"
BASE COUNT          194 a          331 c          325 g          265 t
ORIGIN      
        1 tcatgtcatc ccacggggct cccatccgct tgcccacggc cagaagaatt tcccagtcag
       61 gccttgcctc gccgggtgaa tccaccgctt tccgcaccct ctggacggcc ctgctggtat
      121 tggtgaaggt cccgtccttc tccccccagc tggaggcggg aagcaccacg tgggccagtt
      181 cggcggtttc ggtgaggaaa atatcctgga cgacgaggaa ctcaagattc ttcagggcat
      241 ggcggacatg gcctgaatcg ggttccgtca ccatggagtt ttcacccatg atgtacaggc
      301 ccctgatttt cccttctccg gcggcctcca tcatctcgac cacggaaagg ccaggagttt
      361 cagggatatc ggcgccccag acatccttcg ctttctgtct cgctgccgga agggcaacct
      421 tctggtatcc gggaataacg tcgggaaggc agcccatgtc gcaggctccc tggacgttgt
      481 tctgtcccct gagggggttc actcccgtgc cgggccgtcc cagcattcca cagaggagag
      541 ccaggttcgc cactgtccgg acattgttcg ttcccgtcac gtgctgggtg attcccatgg
      601 tgtagaagat ggcggaattc ggtcccctgg cgtatgccct tgcggcgtcc tcaatgagtt
      661 ccggagcgac gccggtgatc ccggagacat actccggggt gtaccgtgcc acggtctccc
      721 tcagcttatc aaagttgttc acgttctttt cgacgaattc cgccgcatgg agattctcgc
      781 ggatgatcac atgcatcagt ccgttgagga gggctacgtc ggaaccgctt ttctgccgga
      841 tgtaaacatc ggcatatttc gccatttcga ttttccgggg atcgcagacg atgagggtgc
      901 acttgccctt ccgcttctgc tccttgatga aggaaccgat gacggggtgg ttttcagtgg
      961 tgttcgagcc gatgatgaga atggtgtcgg ccgacttgat ggattcgaag tcattggtca
     1021 ttgcgccgct tccgagggtc tcgccgagac ccgcaactgt ggcgctgtgt cacagatggg
     1081 cacagtggtc gacgttattg gtgcccatca cctcc
//
LOCUS       DAOTHD010000885         1114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_156969_length_1114_cov_3.124646, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000885 DAOTHD010000000
VERSION     DAOTHD010000885.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156969_length_1114_cov_3.124646"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="P5219_12205"
     CDS             complement(<1..814)
                     /locus_tag="P5219_12205"
                     /inference="COORDINATES: protein motif:HMM:NF013482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRX27576.1"
                     /translation="MTDFSVVGKPVLKVDSLSLACGAARYTADFEVKDPFYLVFFYSP
                     HAHAEILSIDDGPARGMEGVVDVFHSGNTPSDLYTTAGQGYPEPSPYDTALFNKSVRY
                     VGDIVGAVLAESPAAAREAAGAVRVEYRQLEPLFDPEKSMDPGVPCLHGDGAFAPLPV
                     PYRPEENVAGEVLFSFGDVEKGFAEADFVEEETYRTHYTNHCAMEPHAATATFDEKGR
                     LVIYSSTQVPFHARRTVSRVTGIPAHMIRVVKPRIGGGFGSKQEIILEPYVAL"
     gene            complement(807..>1114)
                     /locus_tag="P5219_12210"
     CDS             complement(807..>1114)
                     /locus_tag="P5219_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRX27577.1"
                     /translation="VNSCQVFAASAQEKEILTVAGLGSIHSPHPLQAAFVETGAVQCG
                     FCTPAMILAAYCLLKKTPNPTDDEIRRALDGNSCRCTGYVKIIEAVRLAARRMSSHD"
BASE COUNT          230 a          333 c          341 g          210 t
ORIGIN      
        1 ccagggcgac gtagggctca aggatgatct cctgcttgct gccgaagcct ccgccgatcc
       61 tgggctttac aaccctgatc atgtgggcgg gtatacccgt caccctgctg accgttctcc
      121 gggcatggaa gggaacctgg gtcgatgagt agatgaccag cctgcctttt tcgtcaaagg
      181 tggccgtggc cgcatggggt tccatggcgc agtggtttgt atagtgggtc ctgtaggttt
      241 cctcttcgac aaaatccgcc tcggcgaaac ccttttccac gtcgccgaag gaaaaaagca
      301 cctcccccgc gacattctct tccggccggt acggcacggg aagaggagcg aaagcgccat
      361 ctccgtggag acagggtacc cccggatcca tggatttttc cgggtcgaag aggggttcga
      421 gctgcctgta ctccacccga acggcacctg ccgcctcccg ggcagccgca ggagattccg
      481 ccagcaccgc accgacgata tcgcccacat agcgaacact cttgttgaag agggcggtat
      541 cgtagggaga aggttcggga tacccctggc cggccgtggt atagaggtcc gacggggtgt
      601 tgcccgagtg aaagacgtcc acgacgccct ccatgccccg cgcgggaccg tcgtcgatgg
      661 acagtatctc cgcatgggcg tggggggaat aaaagaatac aaggtagaaa ggatccttta
      721 cctcgaagtc tgcggtatac ctggcggccc cgcaggccag ggaaagggaa tccaccttca
      781 ggaccggctt tccgaccacg gagaaatcag tcatggctgc tcatcctcct tgccgcgaga
      841 cggaccgcct cgatgatctt gacatacccg gtacagcggc atgaattgcc gtcaagagcc
      901 ctgcggatct cgtcatccgt agggttcggc gtctttttca gaaggcagta ggcggccagg
      961 atcatagccg gagtgcagaa accgcactga acggctcccg tctcgacaaa agcggcctgc
     1021 agcgggtggg gcgaatggat tgatcccagc cccgccacgg tgagaatctc cttttcctgg
     1081 gcggacgcgg caaagacctg gcaggaattc acca
//
LOCUS       DAOTHD010000886         1114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_157058_length_1114_cov_2.075543, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000886 DAOTHD010000000
VERSION     DAOTHD010000886.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157058_length_1114_cov_2.075543"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="P5219_12215"
     CDS             <1..482
                     /locus_tag="P5219_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HRX27578.1"
                     /translation="NLDESQTDEHLPLLESLRSLASEKGLSFVRVFGKMEMELADLSP
                     EEQDEFMQDLGVKEPGRNRLITEAYRLLGLISFFTSGKDEVKAWTLRHGETAVDAAGT
                     IHSDLARGFIRAQVVSYEDFTNHDASLAKCREKGVLRLEGKEYKVADGDMIEIRFNV"
     gene            532..969
                     /locus_tag="P5219_12220"
     CDS             532..969
                     /locus_tag="P5219_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27579.1"
                     /translation="MAMPMQVSLDEILSMLLARVDGLALSDENSKSKFNIITRILYKK
                     GIITDQDVIDSIREEHRMLKSLGLIAEEPEEDVVESIAANILQWIKGDVDALKKGMEE
                     YEKKMQEAMNRQNQKPKIDVASPAVLQQLDRMGGKQGGSKLIL"
BASE COUNT          301 a          259 c          303 g          251 t
ORIGIN      
        1 ccaatctcga cgaaagccag actgatgaac acctccctct tctggaatca cttcgttccc
       61 tggcatctga aaaggggctg agctttgtca gggttttcgg aaaaatggaa atggagcttg
      121 ccgacctctc accggaagaa caggatgaat tcatgcagga cctgggagtg aaagaaccgg
      181 gaagaaaccg gcttatcacg gaagcgtaca ggcttctcgg cctcatctct ttcttcacat
      241 ccggcaagga tgaggtaaag gcctggacac tccgccatgg agagacggcc gttgatgccg
      301 ccggaaccat tcactccgac cttgcgagag ggtttatccg ggcacaggtg gtttcctatg
      361 aggacttcac caaccatgac gcgtctctcg cgaagtgcag ggaaaaagga gtccttcgcc
      421 tggaagggaa agaatacaaa gtcgctgacg gggatatgat agaaatacga ttcaatgttt
      481 agtatggtaa aatcatggtt caaatttttc ttttagagga ggcgcttttt tatggccatg
      541 ccgatgcagg taagtctcga cgaaattctc tccatgctcc tcgcgagggt tgacggcctc
      601 gccctgagcg acgagaattc aaagagcaag ttcaatatca tcacccggat actgtataaa
      661 aaaggaataa tcactgacca ggatgtgatt gattctatca gggaagagca ccggatgctc
      721 aagagcctcg gcctcattgc ggaagagccg gaagaggacg ttgtcgagtc tattgccgcc
      781 aacattctcc aatggatcaa gggtgacgtg gacgccctga aaaagggtat ggaagaatat
      841 gagaaaaaga tgcaggaagc tatgaacagg caaaaccaga aaccgaaaat tgacgtcgca
      901 tctcccgcag tcctccagca gctcgaccgc atgggaggaa agcagggcgg gagcaagctc
      961 atcctttaag ttacggtttt aacaaaaaaa cggtcccggc gggaccgttt ttttttccta
     1021 tttttttgct gtccgtataa ttcgttcaat ttcggagacc aggggtttga tcctcgggcc
     1081 ggaagcgctt ttgttgaggg cctgggcagc ggaa
//
LOCUS       DAOTHD010000887         1113 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_157227_length_1113_cov_2.485822, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000887 DAOTHD010000000
VERSION     DAOTHD010000887.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157227_length_1113_cov_2.485822"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="P5219_12225"
     CDS             complement(<1..774)
                     /locus_tag="P5219_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin oxidoreductase"
                     /protein_id="HRX27580.1"
                     /translation="MGLEDRVLTGVHFMQGNYAAVEGAIAAGCDFFAGYPITPANEVS
                     EGMARRLPAVGGVFFQGEDELCSIYAISAASLAGAKAMTATASAGYDYMQEGIEYAVA
                     VEAPIVVVDVMRCRGENFATQSDIMQVRWAAAGDHEMIVLAPSTVQETFDFTVRAFNL
                     AEEYRTPVVVVSEMSLALMRERLEIPAASAVPVVNRKRTAKSPADYLPFHADSEYGVP
                     DFAELGTGYRVIHSINPHDERGGDRRRGAPTRCLPARRGG"
BASE COUNT          234 a          341 c          346 g          192 t
ORIGIN      
        1 acccccacga cgagcgggga gacatcgagt gggagcccca cgccttcgat cgcctccccg
       61 ctcgtcgtgg gggttgatgg agtggatgac ccggtagccc gtgccgagct ccgcgaagtc
      121 cggaaccccg tactctgaat cggcgtggaa aggaaggtag tcggcggggg acttggcggt
      181 ccgtttccgg ttcaccaccg gaacggccga ggcagcggga atctcgaggc gctctctcat
      241 gagggcgagg gacatctcgg aaacaacgac cacgggggta cggtactcct cggcgagatt
      301 gaaggcccgg acggtgaagt cgaaggtctc ctggaccgtt gaaggagcca gcacgatcat
      361 ctcatggtcg ccggcggcgg cccaccggac ctgcatgatg tccgactggg tggcgaagtt
      421 ttcgccccgg cagcgcatca cgtcaaccac cacgatgggg gcctccacgg ccacggcgta
      481 ctcgataccc tcctgcatgt agtcatatcc tgcactggct gtggccgtca tggccttcgc
      541 cccggcaagg gacgccgcgg atatggcata gatggaacac agttcgtcct ctccctggaa
      601 gaagacgccc ccgacggcgg ggagcctcct ggccatccct tcggagacct cgttcgcagg
      661 ggtgatggga tacccggcga aaaagtcgca tcccgccgca atggcgcctt cgactgcagc
      721 atagtttccc tgcatgaaat ggacgccggt caaaacccgg tcttcaagtc ccattcatac
      781 acctccattt tgtatgcccg ggcaaaatcc gggcagaata caccatgagg agagattacc
      841 acgcatatta tattatgtca aatatcagac cagcagccgg gcaggaaggt ccggctttcc
      901 tcctgcagct gcaggaaaaa ggcggcccga aggccgcccc ggaggatatg accacgatca
      961 gatatgaagg aagggaagaa tgggtttccc gaggattttc tccgccggct caaagagccc
     1021 gggagagcgc atgcctcccc cgagaaccag ctccctcctg gaggcgatga agatcacggc
     1081 attcatcccc ggctggaggc tgtcgctggg gat
//
LOCUS       DAOTHD010000888         1111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_157597_length_1111_cov_3.285985, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000888 DAOTHD010000000
VERSION     DAOTHD010000888.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157597_length_1111_cov_3.285985"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="P5219_12230"
     CDS             <1..409
                     /locus_tag="P5219_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRX27581.1"
                     /translation="LTTPLQIARLYAVIANGGKLVRPHLSSTAGVVARNLSLEKKNLD
                     VIRKGLDYVVRRGTGRQAGSFGITVAGKTGTAQNAHGDDHALFAGYAPMDNPKYVAVA
                     LIEAGLHGSSVASPMVGEILSYLLVPEARGKAQ"
     gene            476..>1111
                     /locus_tag="P5219_12235"
     CDS             476..>1111
                     /locus_tag="P5219_12235"
                     /inference="COORDINATES: protein motif:HMM:NF015716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HRX27582.1"
                     /translation="MSHPLEHREVPAASGDILLKGAGYGIRMIFPETGSEDKLLSDLE
                     KLFGEASLLSGNLGVVFDFQGRKVSRYFIQKLLSDFIWSRKMKILSWISLDGETLDDL
                     RSMGVATGEPVPERSGKKEKTSGNALFLPRSLRSGQRVEHEGDVLVIGHVNDGAEVFA
                     SGSICVWGKLKGLAHAGLDGGEDHTVVAGLFEAKQVRIGGKVSSSLGSSMEW"
BASE COUNT          232 a          317 c          359 g          203 t
ORIGIN      
        1 gctcaccaca cctctccaga ttgcgaggct ctatgcggtg atcgccaacg gcggcaagct
       61 ggtgcgcccc catctgtcct ccacagccgg agtggtcgcc aggaatctgt ctcttgaaaa
      121 gaaaaacctg gatgtcatca gaaagggtct cgattacgtg gtcaggcgcg gaacaggccg
      181 gcaggcaggc agcttcggca tcaccgtggc ggggaaaacg ggtacggccc agaatgccca
      241 cggagacgac cacgccctgt tcgcgggcta cgctcccatg gacaatccga agtacgtggc
      301 ggtggccctt atcgaagcgg gccttcacgg cagctcggtg gcatcgccca tggtgggtga
      361 gatcctttcc tatctgctcg ttcccgaagc gcggggaaag gcccagtgaa cgagagcgca
      421 ggaacggtac aatcaggcgg aactctccgc cgtagaattc tgaggaggtg cggtcgtgtc
      481 ccatcccctt gaacacaggg aagtcccggc ggcttcagga gacatacttc tgaaaggcgc
      541 cggatacgga atacggatga tctttcccga gacgggaagc gaggacaagc ttctttccga
      601 cctggaaaag ctgttcggag aggcttccct gctatcgggc aacctcggcg tggtctttga
      661 tttccaggga cggaaggtgt ccaggtattt catccagaag cttctttccg atttcatctg
      721 gtcccggaag atgaagattc tctcgtggat ttccctggac ggggaaaccc ttgacgacct
      781 ccggtccatg ggggtggcca ccggcgaacc cgtgccggag cgatccggaa agaaggaaaa
      841 gacatcgggg aacgccctct tcctgccccg ctccctccgg tccgggcaga gagtggagca
      901 cgagggggac gttctcgtca tcggccacgt aaacgacgga gcggaagtct tcgcctcggg
      961 cagcatctgc gtgtggggca agctgaaggg gctcgcccac gccggtctcg acggcgggga
     1021 agaccacacc gtggtggcgg gactcttcga ggcaaaacag gtgcggatcg gcggaaaagt
     1081 gagctcttcc ctgggaagtt ccatggaatg g
//
LOCUS       DAOTHD010000889         1111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_157624_length_1111_cov_2.809659, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000889 DAOTHD010000000
VERSION     DAOTHD010000889.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157624_length_1111_cov_2.809659"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1111
                     /locus_tag="P5219_12240"
     CDS             <1..>1111
                     /locus_tag="P5219_12240"
                     /inference="COORDINATES: protein motif:HMM:NF023400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27583.1"
                     /translation="RVILAAGMTSDAGPLGIQKEVRQEVTVTRKMEIRGAYASSNYPE
                     QSGITISTSIGADMASAARFVELSPKIPFTVEPGYNGFTILGDFKPRQRFEVTLKKGL
                     PSRDGTKLEADYTKAVIFPDMYPALLFPAAGTFLSPAGDLRVPIETVNIEEVSLNLWR
                     VYENNIPLAMTMQGYSVPRDLTRRIVTKNARPEGALNTPVRRAIDLRELAGEAKGVFL
                     LSASDVSGEYWGEAEQLVAVTDLGITARVAPAGITVWVNTILGMKPVDSASVKVYSKS
                     NQLIASGATDGVGIWSWSGKDSWDPQLVPAIVTVEKGDDLSFLKLDTNLLADGGFDTG
                     GRSWSTGYDAMVFPPRGVFRPGEQVDFSAIVRDARR"
BASE COUNT          236 a          367 c          326 g          182 t
ORIGIN      
        1 ccgggtgatc ctggccgccg gaatgaccag cgacgccggg cccctgggaa tacagaagga
       61 agtacggcag gaagtgacgg tcaccaggaa gatggaaata cggggagcat acgcttccag
      121 caactaccct gaacagagcg gcatcaccat cagcacctcc atcggtgcgg acatggcctc
      181 ggcggcccgg ttcgttgagc tttccccgaa aatacccttc accgttgagc cggggtacaa
      241 cggcttcacc atcctgggag acttcaagcc caggcagcgc ttcgaagtga ccctgaaaaa
      301 agggcttccc tccagggacg gcacaaaact cgaagccgac tacacgaagg cggtcatctt
      361 ccccgacatg tatcccgccc ttctcttccc cgccgccggt accttcctct cacccgccgg
      421 cgacctgaga gtccccatcg aaacggtcaa tatcgaggaa gtcagcctga acctctggag
      481 ggtctacgag aacaacatcc ccctcgccat gaccatgcag ggatattccg ttccccggga
      541 cctcacgagg agaatcgtca cgaagaacgc ccggcccgaa ggagcgctga acacccccgt
      601 ccgcagggcc atcgacctca gggagcttgc cggtgaggca aagggcgtct tccttctctc
      661 agcctccgat gtctccggtg aatactgggg cgaagccgaa cagctcgtgg ccgtgaccga
      721 cctggggatc accgcgagag tcgccccggc ggggatcacc gtctgggtga acaccatcct
      781 cgggatgaag cccgtggaca gcgcttccgt caaggtatac tcaaagagca accagctgat
      841 cgcatcggga gccaccgacg gcgtcggcat ctggtcctgg agcgggaagg attcctggga
      901 tccccagctc gtccccgcca tcgtcaccgt ggaaaaggga gatgacctct ccttcctgaa
      961 gctggatacc aatctcctcg ccgacggggg gttcgacaca ggcggcaggt cctggagcac
     1021 cgggtacgac gccatggtgt tccccccgag aggagtcttc cggcccggtg aacaggtgga
     1081 tttctccgcc atcgtccggg atgcccgcag g
//
LOCUS       DAOTHD010000890         1111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_157713_length_1111_cov_1.605114, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000890 DAOTHD010000000
VERSION     DAOTHD010000890.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157713_length_1111_cov_1.605114"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="P5219_12245"
     CDS             complement(<1..1024)
                     /locus_tag="P5219_12245"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRX27584.1"
                     /translation="MKYFPFGKTGISLSEVGFGAIPIIRLSFAEAERTLRRAYDLGIT
                     LFDTANAYHDSEEKIGRALSPVRDTIFLATKTMKRDAASAAEHIDLSLRRLNTDYIDL
                     FQFHQVALEKDWEKIMAPGGAMEAVLEAQKAGKIRFIGITSHSLSMAKKYVESGLFVS
                     IQFPFNFIETDAAKELHPLAREKGVAILAMKPFAGGAITDAALAFKYLRSHGNLFPIP
                     GYDSAESVEQIVAFYEKPNEVTGNDLAAMEAVRSELGGQFCRRCEYCQPCPQGVSITQ
                     GMIYPLLASRMSPEVASEFASAGMDTLTKCTECGACVKKCPYTLPIPAMLKKHYELYL
                     GHRKAKA"
BASE COUNT          188 a          318 c          372 g          233 t
ORIGIN      
        1 aggcctttgc cttcctgtgg ccgaggtaca gttcgtaatg cttcttcagc atggccggaa
       61 ttgggagggt gtaggggcac tttttcacgc aggccccgca ttccgtgcac ttcgtcaggg
      121 tgtccatgcc cgcggaggcg aactcagagg ccacctcggg tgacatgcgg ctcgccagca
      181 gggggtagat catgccctgg gtgatggaga ctccctgcgg gcagggctgg cagtactcgc
      241 agcggcggca gaactggcct cccagttcgc tccttaccgc ctccatggcg gcaaggtcat
      301 tgcctgtgac ttcgttcggt ttctcgtaga acgcgacgat ctgctccacc gattccgccg
      361 agtcgtaccc ggggatgggg aagaggttcc cgtgggagcg gaggtacttg aaggccaggg
      421 ccgcgtcggt gatggccccg cccgcgaagg gtttcatggc gaggatggcg acgccctttt
      481 cccgggcgag ggggtggagt tccttcgccg cgtcggtctc gatgaagttg aaggggaact
      541 ggatggagac gaacaggccc gattccacgt acttcttcgc catggagagg ctgtgggaag
      601 tgatgccgat aaagcggatc ttccccgcct tttgtgcctc caggaccgcc tccatggcgc
      661 ctcccggagc catgatcttc tcccagtcct tttcgagggc cacctggtgg aactggaaaa
      721 ggtcgatata gtcggtattc agccgcctca ggctcaggtc gatgtgctcc gcggcggagg
      781 cggcgtcccg cttcatggtc ttggtggcga ggaagatggt gtctctcacc ggtgaaaggg
      841 cccggccgat tttctcctcg ctgtcatgat aggcgttcgc cgtgtcgaag agggtgatgc
      901 ccaggtcgta ggcccggcgg agggtccgct cggcctcggc gaaggacagc cggatgatgg
      961 gtatggcccc gaatcctacc tcagacaggg aaattcccgt cttcccgaag ggaaagtatt
     1021 tcatggtcgt cagctccttc tcgccggacg gtttccggca tttcaccaca ggatagcaga
     1081 caagtccccg gaccggaagg gaggggctat t
//
LOCUS       DAOTHD010000891         1110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_157771_length_1110_cov_3.402844, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000891 DAOTHD010000000
VERSION     DAOTHD010000891.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157771_length_1110_cov_3.402844"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..>1110
                     /locus_tag="P5219_12250"
     CDS             23..>1110
                     /locus_tag="P5219_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRX27585.1"
                     /translation="MLQVARRNDIYIPTLCWMEGLTPIGSCRMCVVEVEGNPKLLTAC
                     TTPAENGMKIHTKTEKLKQYRLQILELLFAGRNHFCMFCSQTGDCELQRLAIEHGMDK
                     VRYPFLYEPFHNDATDPNIQLDHNRCILCLRCIRVCAEKVGAHTLDLEKRGWGTNITA
                     DLGKMLGESDTCVSCGACAQVCPTGTITLRDFVYRGRRNDCDDQVESICPLCSLGCRI
                     KAYVRTGSVARVEGLSANSPDGGQLCHKGRWGLPKSTERDRLTAPMIRTGSSFREASW
                     EEALNLIASKLGSASKAGRAGALLSELSTDEELSVVSSFFRDTMKMDKVDGFFGNMLR
                     GFVKGFQPFADQGIRPFTGAHNILGSDLV"
BASE COUNT          219 a          364 c          341 g          186 t
ORIGIN      
        1 cagggcggca aaaggagaca ccattctcca ggtcgcgcgg agaaacgaca tctacattcc
       61 gaccctctgc tggatggagg gactgacgcc catcggctcg tgccgcatgt gcgtcgtgga
      121 ggtggagggg aaccccaagc tcctcaccgc ctgcaccaca ccggcggaga acggaatgaa
      181 gattcatacg aagacggaaa agctgaaaca gtaccgcctg cagatcctgg agcttctctt
      241 cgcgggaaga aaccacttct gcatgttctg ctcccagacg ggggactgcg aactccagcg
      301 gctcgccatc gagcacggca tggacaaggt ccgctacccc ttcctgtacg agccgttcca
      361 caatgacgcc acggacccga acatccagct ggaccacaac cggtgcatcc tctgcctgag
      421 gtgcatccgg gtctgcgccg agaaagtcgg ggcccatacc ctcgaccttg aaaaacgggg
      481 atggggaacc aacatcaccg ccgatctcgg gaagatgctc ggcgagagcg acacgtgcgt
      541 gtcctgcggc gcctgcgccc aggtctgccc cacggggacc atcaccctcc gggatttcgt
      601 ctaccgggga cggcggaatg actgcgacga ccaggtggag agcatctgcc ccctctgttc
      661 cctcggctgc aggatcaagg cctatgtccg cacggggagc gttgcccggg ttgaaggcct
      721 ttcggcgaac agccccgacg gcggccagct ctgccacaag gggcgctggg gcctgccgaa
      781 atccaccgaa cgggaccgcc tcacggctcc catgatccgt acgggctctt ccttcaggga
      841 ggcttcctgg gaggaggccc tgaacctcat cgcatcgaag ctcggctccg catcgaaggc
      901 cggcagggcg ggcgcccttc tttcggagct gtccaccgac gaggagcttt cggttgtctc
      961 ctccttcttc cgggacacca tgaagatgga caaggtggac ggcttcttcg gcaatatgct
     1021 ccggggattc gtgaaggggt tccagccctt cgccgaccag gggatacggc ccttcaccgg
     1081 ggcgcacaac atcctcggga gcgacctcgt
//
LOCUS       DAOTHD010000892         1110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_157778_length_1110_cov_3.298578, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000892 DAOTHD010000000
VERSION     DAOTHD010000892.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157778_length_1110_cov_3.298578"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="P5219_12255"
     CDS             complement(<1..1096)
                     /locus_tag="P5219_12255"
                     /inference="COORDINATES: protein motif:HMM:NF037270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRX27586.1"
                     /translation="MKDQGNEHDTGMNLFFLTDPGDLSATLSSGGGLPDGFILGRCGS
                     GSECGHMLRELRKGDETALLPVFLEDFPSDGDREAADGPARTKEEMENLAREIGNRLR
                     DVDENALRKSLDLRLLAFLYSREGKKLRPVLDPLSRELYSYPLADHLSGGLLDSSRWI
                     SELLEKGYLEEESLADRIRACPKCGGAHLNYVDVCPFCRSMDIRQVPFIHCFTCGRVG
                     PQEAFVREGPMQCPFCGARLRHIGSDYDRPMDNYLCSSCSQTFAEPDVSCRCFLCGAW
                     SPADGLSVRSFREYSLSDKGVIAVRSGNTDDMYSVFDALNYTKPEPFRHILDWQLQLS
                     GRPPVEPFSLMIISFLNVEKVKEKLGRKKVT"
BASE COUNT          239 a          336 c          309 g          226 t
ORIGIN      
        1 ccgtcacctt tttccggccc agcttttcct tgactttttc aacgttgagg aaggagatga
       61 tcatgaggct gaaaggctcc acgggaggcc tacccgagag ctggagctgc cagtcgagga
      121 tatggcggaa cggctccggc ttggtatagt tcagggcgtc gaatacggaa tacatgtcat
      181 cggtgtttcc gctcctgacg gcaatcaccc ccttgtctga aagggaatac tccctgaagg
      241 agcgcaccga cagcccatcc gcggggctcc atgcgccgca gagaaaacac ctgcacgaga
      301 catccggttc cgcaaaggtc tgggaacagg agctgcagag ataattgtcc atgggcctgt
      361 cgtaatccga accgatatgg cgcagccttg ccccgcagaa gggacactgc atgggcccct
      421 ccctgacaaa ggcctcctgg ggacctaccc ttccgcacgt gaaacagtga atgaagggaa
      481 cctggcggat gtccatgctt ctgcagaagg ggcagacatc cacgtaattc aggtgggcgc
      541 cgccgcattt cgggcacgcc ctgatcctgt cggcaagaga ctcttcctcg aggtagcctt
      601 tctccagcag ttcggatatc cacctggacg agtcaagcag ccctccggag agatgatctg
      661 caaggggata ggaataaagc tccctggaaa gagggtcaag aacaggcctc agctttttgc
      721 cctcccggga atagagaaag gccaggagcc tgagatcgag acttttacgg agggcatttt
      781 catccacatc cctcagcctg ttcccaatct cccgggccag attctccatc tcctctttcg
      841 tcctggccgg tccgtccgcc gcctcccggt caccgtccga aggaaaatcc tccagaaaaa
      901 cgggcagaag ggccgtttcg tctcccttcc ggagctccct gagcatatgg ccgcattcgc
      961 tgccggaccc gcacctgccg aggataaagc cgtcagggag ccccccgccg gatgagaggg
     1021 ttgcggaaag gtccccggga tccgtgagaa agaaaaggtt cattcctgta tcatgctcgt
     1081 ttccctggtc tttcatgctt tccctcctgt
//
LOCUS       DAOTHD010000893         1108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_158233_length_1108_cov_2.704653, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000893 DAOTHD010000000
VERSION     DAOTHD010000893.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158233_length_1108_cov_2.704653"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="P5219_12260"
     CDS             <1..545
                     /locus_tag="P5219_12260"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRX27587.1"
                     /translation="CRHLHLPLQSGSGSVLARMKRGYTPGEFLSLAEKARRFLGDDLH
                     ISTDVLVGFPGESEGDFDDTLSLMKACRFGKVHVFPFSPREGTEAWSYPDKIPRETVL
                     RRTREALSAADGLLAGFASRWVGSDVPVLVEETAGNSFQGLTPSFLRVAARGGAERGE
                     ELPVRITRISGDSLRGRRVP"
     gene            542..>1108
                     /locus_tag="P5219_12265"
     CDS             542..>1108
                     /locus_tag="P5219_12265"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRX27588.1"
                     /translation="MRTSGPFSPSDGPVLGIDLGTRYALCAVFDEARGAVVIPNRWGK
                     RTTPSVTAWTGSGWTAGETAAAGEMKRPSTTWWDLKRKVGTPWKGWCGRTSVSAEDAL
                     VPLLTLLREDGEVFLGTFVESCVLAVPASFSFAERSAMARAARAAGFSKSRIVNEPTA
                     AALAFGASGRFLILDYGAGTVDLSVVECG"
BASE COUNT          172 a          339 c          387 g          210 t
ORIGIN      
        1 tctgccggca ccttcacctt cccctccaga gcggaagcgg gtccgtgctg gcccggatga
       61 agcgggggta cactcccggg gaatttctgt ccctggcgga gaaggcgaga cgttttctgg
      121 gggacgacct ccatatcagc accgatgtgc tcgtgggatt tcccggcgag agcgaaggcg
      181 atttcgacga caccctttcc ctgatgaagg cctgccgctt cggcaaggtg catgttttcc
      241 ccttttcacc cagggagggg acggaggcgt ggtcctatcc ggacaaaata ccgagggaaa
      301 cggtgctccg gcggacacgg gaagcgctct cggcggcgga cggactcctg gcgggattcg
      361 cttcccggtg ggtgggctca gacgttcccg tcctcgtgga ggaaactgcc gggaattcct
      421 tccagggtct cacgccgtcc ttcctgagag tggcggccag gggcggagcc gagcggggag
      481 aagagctgcc ggtgagaatt acccggatct ccggcgattc cctgagaggg cggagggtgc
      541 cgtgaggacc tccggtccgt tttccccctc tgacgggccc gttctcggaa tcgatctcgg
      601 aacgaggtat gccctctgcg ccgttttcga cgaagcccgg ggagccgttg tgattccgaa
      661 ccgctgggga aagcggacta ctccttcggt cacggcctgg acgggaagcg ggtggacggc
      721 tggggagacg gctgcggccg gagaaatgaa gcgcccctcc acaacgtggt gggacctgaa
      781 gcgcaaggtc ggcacaccgt ggaaaggatg gtgcggcagg acctccgtgt ccgcagaaga
      841 tgccctcgtg ccgctgctga cccttctccg ggaggatggg gaggtttttc tcggcacctt
      901 tgtcgagtcc tgcgtccttg ccgtccctgc ctccttcagt ttcgccgagc ggtccgccat
      961 ggcccgggcg gcccgggccg ccggattttc gaagtccagg atcgtcaacg aaccaacggc
     1021 ggcggccctc gccttcggcg cttcgggccg gtttctcatc ctcgactacg gcgccggtac
     1081 cgtggacctg tcggtggtgg agtgcggg
//
LOCUS       DAOTHD010000894         1107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_158402_length_1107_cov_3.547529, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000894 DAOTHD010000000
VERSION     DAOTHD010000894.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158402_length_1107_cov_3.547529"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..456
                     /locus_tag="P5219_12270"
     CDS             7..456
                     /locus_tag="P5219_12270"
                     /inference="COORDINATES: protein motif:HMM:NF023270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
                     /protein_id="HRX27589.1"
                     /translation="MRLAEFLRDIRFQPEQVQDFIPTPGSLSTCIYYTGIDPFSGKKV
                     HVPKDRNERKMQRALLQYREPKNRETVLQALNAAGRPDLVGEGPLCLVRSQQKKENRR
                     GKETPPAGSAAKRKREGMSTVSLRQGAGRKKPSRVPGRSPSAGRRPG"
     gene            complement(368..>1107)
                     /locus_tag="P5219_12275"
     CDS             complement(368..>1107)
                     /locus_tag="P5219_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRX27590.1"
                     /translation="RKRLAGVSFHDLKAGVIIGVFLFLGFSTQTLGLNYTTPGKQAFL
                     TATYVVIVPLLSWVIRRRFPGILSFIASVVCLAGMAMLTLQEGLAVGAGDTLTLVCAV
                     FFACHILAIEHFAAKTDPVILAIIQIFTVGMLSFPFALAFETWPGFNGGSGLWSIAFT
                     VLFCTVFAFAAQNVAQKFTPSTHTAIILSLESLFGALAGIYFVGEIFTARMAAGCALI
                     LAAVLLTETGPALAKAFSGRLPAGEKP"
BASE COUNT          273 a          302 c          341 g          191 t
ORIGIN      
        1 gatgcggtgc gcctcgcgga gtttctgcgg gacatcagat ttcagcccga acaggtccag
       61 gatttcatac ccactcccgg aagcctgtcc acctgcattt attacacggg aatcgatccg
      121 ttttccggaa agaaggtcca cgtgccgaag gacaggaatg aacggaaaat gcagcgggct
      181 cttctgcagt accgggagcc gaagaacagg gagaccgtac tgcaggctct gaatgctgcc
      241 ggccggcctg atctcgttgg agaaggcccg ctctgtcttg tacggtccca acaaaaaaaa
      301 gaaaaccgcc ggggaaagga aactcctccg gcaggcagcg ctgctaaaag aaagcgggaa
      361 ggaatgtcta cggtttctct ccggcaggga gccggccgga aaaagccttc gcgagtgccg
      421 ggccggtctc cgtcagcagg acggcggcca ggatgagggc acagcctgcg gccatcctgg
      481 ccgtgaaaat ttctcccacg aaatagattc ccgcaagggc tccgaagagc gactccaggc
      541 tcaggatgat tgcggtgtgc gtagacggtg tgaatttctg ggccacgttc tgcgcggcga
      601 aggcgaaaac ggtgcagaag agtaccgtga aggcaatgct ccacaatccg gatccgccgt
      661 tgaagcccgg ccatgtctcg aaggccaggg cgaagggaaa actcagcatt cccacagtga
      721 agatctggat tatggccagg atcacggggt cggttttcgc ggcgaagtgt tctatcgcca
      781 gtatatgaca ggcgaagaaa acggcacaga ccagcgtgag tgtgtccccg gcaccgactg
      841 ccagcccttc ctggagagtg agcattgcca tgcccgcaag gcagacgact gacgcgatga
      901 aagagagtat tcccggaaac cgcctcctga tgacccagga gagcagggga acgataacca
      961 cgtaggttgc ggtaagaaat gcctgcttcc ccggtgtcgt gtagttcaga ccgagggttt
     1021 gggtggaaaa cccgagaaac agaaaaaccc cgatgatcac gcctgccttc aggtcgtgaa
     1081 aggaaacccc cgcgagcctt ttccgga
//
LOCUS       DAOTHD010000895         1107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_158416_length_1107_cov_3.064639, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000895 DAOTHD010000000
VERSION     DAOTHD010000895.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158416_length_1107_cov_3.064639"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1107
                     /locus_tag="P5219_12280"
     CDS             <1..>1107
                     /locus_tag="P5219_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRX27591.1"
                     /translation="DGLGLAGVVADGGTAAGTFREYQRSVTLSMNGSGLADIAAGLDS
                     LTGLSASVTGGGGLPASVQMAGHANYDVSLASAGQIAAELSLDHVFDGGGSHSSAQVW
                     NGRGVIADTGGKNLADAALSIQNAVGGIPGMAAFAAGTTASGNLHGLSFSNAVGIYEI
                     SMTGRAIRELSTGVYGPPDSITVGRGGGFSTGNRVYVNHEVSVSTFSADAMEVVSALN
                     SAVGGALGLDGLTQNNGISPFSAISAGASRQLRLSSVPSSGYRISVTGGSGGSLQEVW
                     GGQRTVNRGGTSFGQVLDYGKETVFAVTGMNMEEAFGAISSDGRLSLNWLTEPSHTAG
                     EHEGRIAFTNVSAGAGRRVFSMAQAGTDQGNVLN"
BASE COUNT          199 a          337 c          374 g          197 t
ORIGIN      
        1 cggacgggct ggggctggcc ggggtcgtcg cggacggggg gacagcggcc gggacgttcc
       61 gggaatacca gcggtcggtg acgctgtcga tgaacggctc ggggcttgcg gatatcgcag
      121 cggggctgga ctctctcacg ggcctctcgg cttccgtcac gggagggggc ggcctgcccg
      181 cttccgtcca gatggccggc cacgccaact atgatgtctc gctggcctcc gccgggcaga
      241 ttgccgcgga actctccctt gaccatgtct ttgacggagg cggttcccat tcgtccgccc
      301 aggtctggaa cggccgcggg gtgattgccg acaccggcgg aaaaaatctt gccgatgccg
      361 ccctgtccat tcagaatgcc gttggcggaa taccggggat ggcggccttt gcggcgggaa
      421 caaccgcgtc cggaaacctc catgggctca gtttcagcaa cgcggtcggc atctacgaga
      481 tctccatgac aggaagggcg atccgggagc tttcgacagg cgtctacggt ccccccgatt
      541 ccatcacggt cggaagaggg ggcggctttt ccacggggaa cagggtctac gtcaatcatg
      601 aggtttccgt cagcaccttc agcgccgacg caatggaggt ggtctcagcc ctcaactccg
      661 ccgtcggcgg agccctcggt cttgacggcc tgacgcagaa caacgggatc tcgccctttt
      721 ccgccatctc agcaggtgct tccaggcagc tcaggctttc gagcgtccct tcatcgggat
      781 accgcatctc cgtcaccggc ggaagcggag gatccctcca ggaagtctgg ggcggacaga
      841 gaacggtcaa ccggggcgga acgtccttcg gacaagtcct tgactacggg aaggagaccg
      901 tcttcgccgt gaccgggatg aacatggagg aggctttcgg cgcgatatcc tcggatggaa
      961 gactctccct gaactggctg acggagcctt cccataccgc cggggagcat gaaggcagga
     1021 tcgccttcac gaatgtctcg gcgggagcgg ggagaagagt gttttccatg gcacaggccg
     1081 gaacggacca gggaaacgtg ctcaacg
//
LOCUS       DAOTHD010000896         1105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_158879_length_1105_cov_2.651429, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000896 DAOTHD010000000
VERSION     DAOTHD010000896.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158879_length_1105_cov_2.651429"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="P5219_12285"
     CDS             <1..734
                     /locus_tag="P5219_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27592.1"
                     /translation="IRPLSGMNDGSSAAAHVLEYNGGYFHDFERNRKYYFTAAGGETF
                     LVFADIPQYGGDVLFFQKVPVPLTPVHLAADVDGRVWFLRNASPFLQSSDILLVRSSR
                     IAGLPGYIDFFGIKRVESQKYAAIAATGFRDQCSLSLFKKGNETRVKVMQFVFSGEDS
                     VPTISGGKVVIGPEGENEWRRSERGAVLSFVKPEDGRVIVLSGTGDFPLLFDSITDEG
                     EIYVPEGSFLFFAGDSGDIFEVVSR"
     gene            complement(853..>1105)
                     /locus_tag="P5219_12290"
     CDS             complement(853..>1105)
                     /locus_tag="P5219_12290"
                     /inference="COORDINATES: protein motif:HMM:NF014019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HRX27593.1"
                     /translation="SCCLSKSIVQDVSSNLKNWTIGGELNQYRAMIDQSVATVMSRIS
                     DDAQKSGADAVLGFRLSTTSVSSGAAEVIGYGTAVKFMK"
BASE COUNT          238 a          277 c          302 g          288 t
ORIGIN      
        1 atatccggcc cctttcagga atgaacgacg gctcctccgc ggctgcgcac gtgcttgagt
       61 ataacggcgg gtactttcat gatttcgaaa gaaatagaaa gtactatttc accgcggcgg
      121 gcggtgaaac gttcctggtt ttcgcggata ttccgcagta tggcggagat gtccttttct
      181 tccaaaaagt accggtaccg ctgactcctg tccatcttgc cgcagacgtg gacggcaggg
      241 tctggttttt gcggaacgcg tcgccctttc ttcagtcttc cgatatcctt ctcgtccggt
      301 catccaggat tgcgggactt ccgggctata tcgacttctt cggaatcaag agggttgaat
      361 cccagaaata cgccgctatc gcggcaaccg gcttccggga ccagtgctcc ctgagtctgt
      421 tcaaaaaggg aaatgaaacc agggtgaagg taatgcagtt cgtcttctcc ggggaggata
      481 gcgttccgac tatctcaggc ggaaaagtgg tcatcggccc ggaaggagaa aatgaatgga
      541 ggcggtctga acgtggtgct gttctctctt ttgtcaagcc cgaggacggc agggtgatcg
      601 ttctttcggg aacaggcgat tttccgctcc tttttgacag tataacggac gagggggaaa
      661 tctatgttcc cgaaggaagc ttcctgtttt ttgccggaga ttccggtgat attttcgagg
      721 ttgtcagccg gtagatcatt acgggcttgc tgtcttataa tgcaataaca gttgcacatt
      781 tcccccttgc ttagagaaaa aagccctccg ccgggaaaag gcgaagggct tttttttcgg
      841 aaatttccgg agttatttca tgaacttgac cgccgtcccg taaccgatca cttccgccgc
      901 tccggaggaa acgcttgtag tggacagccg gaatccgagt acagcatctg cgcctgattt
      961 ttgggcatca tcactgattc tggacattac cgtggcaacc gactggtcga tcatagccct
     1021 gtactggttc agctctcctc cgatggtcca gttcttcagg tttgaagaaa cgtcctggac
     1081 tatggatttc gagaggcagc acgac
//
LOCUS       DAOTHD010000897         1103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_159327_length_1103_cov_2.292939, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000897 DAOTHD010000000
VERSION     DAOTHD010000897.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159327_length_1103_cov_2.292939"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="P5219_12295"
     CDS             <1..304
                     /locus_tag="P5219_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRX27594.1"
                     /translation="ALLLEGLLTLPGVQLSGRKTMDGRLPVFAVNFEGRDNGILAARL
                     SEDWGIETRPGLHCAPLAHKTLGTFPGGALRLSPGWFSTVDEIRKTVDALKAVLAS"
     gene            325..>1103
                     /gene="selD"
                     /locus_tag="P5219_12300"
     CDS             325..>1103
                     /gene="selD"
                     /locus_tag="P5219_12300"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:358..360,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HRX27595.1"
                     /translation="MRLTELSTTSGUAAKIGPADLEKVLRDLPLPSHERIITSWGSGE
                     DAALWTIDSERAGILTVDFITPVSDDPLTWGQIAAANSLSDVFAMGGKPFVALNIVGF
                     PTKTLELDVLKDVLRGGQEKVAEAGAFLLGGHSVDDREPKYGLVVYGEVRQDSLWKVT
                     GARPGDLLLLTKPVGTGIVATAVKADMVEDEASRTEAARWMTTLNDLPRRLSPDECRM
                     VHACTDVTGFGLAGHVLDMLSEGGLKLTLGIRDIPLLPGVLG"
BASE COUNT          213 a          332 c          369 g          189 t
ORIGIN      
        1 ggcccttctt cttgaaggtt tgcttaccct tcccggagtg cagctctcgg gacggaagac
       61 catggacggg cgcctgcccg tgttcgccgt caactttgag gggcgggaca acggcattct
      121 cgccgccagg ctgtccgaag actggggcat cgaaacgagg ccgggactgc actgcgcccc
      181 tctggcccac aaaacccttg gaactttccc cggcggggcc ctgcgcctca gcccggggtg
      241 gttcagcacc gtggacgaga tccggaaaac ggtggatgcc ctgaaggcgg tgcttgcatc
      301 atgaattgcg gaggaggtgc ggaaatgagg cttacagagc tttcgaccac cagcgggtga
      361 gcggcgaaaa taggtccggc ggacctggag aaggtgctga gggatcttcc gctgccatcc
      421 catgaaagga tcatcacgtc ctgggggtcc ggcgaggatg ccgccctctg gacgatagat
      481 tccgagcggg ccggaatcct cactgtggac ttcatcaccc ccgtgtcgga cgaccccctc
      541 acctggggac agatcgcggc ggcgaactcc ctgagcgacg tcttcgccat gggcggaaag
      601 cccttcgtgg cattgaacat cgtgggcttt ccaacaaaaa ccctcgaact ggacgtgctg
      661 aaggacgtcc tgcggggtgg gcaggaaaaa gtggcggagg cgggagcctt cctcctggga
      721 ggccacagcg tggacgacag ggaacccaag tacggccttg tcgtgtacgg agaggtacgt
      781 caggattccc tctggaaggt aacaggggca cggccgggag accttcttct tctcacgaag
      841 ccggtcggca cgggaatagt cgcgacagcc gtcaaggcgg acatggtgga ggacgaggct
      901 tccaggacgg aagccgcccg gtggatgacc accctgaacg acctcccgag gcgactctcc
      961 cccgacgaat gccgcatggt gcatgcctgc acggacgtca ccggcttcgg gctcgcggga
     1021 cacgtcctgg atatgctttc ggagggcggc ctgaagctca ccctggggat acgagacata
     1081 ccccttctgc caggcgtcct ggg
//
LOCUS       DAOTHD010000898         1101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_159721_length_1101_cov_2.760994, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000898 DAOTHD010000000
VERSION     DAOTHD010000898.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159721_length_1101_cov_2.760994"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1101)
                     /locus_tag="P5219_12305"
     CDS             complement(<1..>1101)
                     /locus_tag="P5219_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HRX27596.1"
                     /translation="KAITVNEDEWDLLRSREGLILMADPTFYVQVGAYSRPRLMKEAL
                     EHHGLRDISEYASTAFDLAAYASAAIIREGGENLPYISTYCPAVIRLIQINFPELVGR
                     ILPVESPLETAVTIWRDATGGGEKVTLVAPCPAKATLVRNPVGRGKSSLEYVVSVKKV
                     IRDLLAYNVKVTGTKTKAISRRWLLWSITGGEAKHISSFSDKGLTSIAVSGLRNTMDL
                     LNELELGRLGGVDFIECRACDLGCIGGTGTYESRFLSQLRLENIETEWLPSHEEMEEI
                     RTWYEKGIWRLENPIQVKERLPLSQDLGEAMAKLREMDAIYAGLPHIDCGSCGRPSCR
                     ALAEDIVRGQGEVTDCIFKLRERITSLSGEIQA"
BASE COUNT          210 a          337 c          313 g          241 t
ORIGIN      
        1 ggcctggatc tctccgctca gggaggtgat ccgctccctc agcttgaaga tgcagtccgt
       61 tacctctccc tggccacgga cgatgtcctc cgccagggca cggcaggagg gacggccgca
      121 ggaaccgcag tcgatgtgcg gcagtcctgc gtaaatggcg tccatctccc tgagcttggc
      181 catggcctcc ccaagatcct gggaaagggg cagccgctcc tttacctgga tcggattttc
      241 caggcgccat atcccttttt cataccaggt gcggatttct tccatctctt cgtgggaggg
      301 aagccactcc gtctcgatgt tttccaggcg gagctgggag aggaaccggg actcgtatgt
      361 tcccgttcct ccgatacatc caaggtcgca ggcccggcat tcgatgaaat ccacccctcc
      421 gagccgcccg agctccagtt cgttgagcag gtccatggta ttcctcaggc cggaaacggc
      481 tattgaggta agccccttgt ccgagaaaga ggaaatatgt tttgcctctc ccccggtaat
      541 ggaccagaga agccacctcc tgcttatggc cttcgtcttc gttccggtga ccttgacatt
      601 gtaggcgaga agatcgcgga tgaccttttt cacgcttacc acgtactcga gagagctctt
      661 ccccctgcct acgggattcc ggaccagggt ggccttcgcg ggacaggggg cgacaagggt
      721 tactttttcc cctcccccgg tggcatccct ccagatggtg accgccgtct ccaggggaga
      781 ttccaccggc aggattcttc cgaccagctc cggaaaattg atctggatga gcctgatgac
      841 ggcagggcag taggtggaga tgtagggaag attctctccc ccttccctta taatggcggc
      901 cgaagcatac gccgcaaggt caaaggctgt ggatgcatac tcggaaatat ccctgagccc
      961 gtgatgctcc agggcctcct tcatcagccg ggggcgtgaa taggccccca cctggacgta
     1021 gaacgtggga tccgccataa ggatcagccc ttccctggaa cggagaaggt cccattcatc
     1081 ctcgttcacg gtgatggcct t
//
LOCUS       DAOTHD010000899         1100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_159900_length_1100_cov_3.673684, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000899 DAOTHD010000000
VERSION     DAOTHD010000899.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159900_length_1100_cov_3.673684"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="P5219_12310"
     CDS             complement(<1..1020)
                     /locus_tag="P5219_12310"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRX27597.1"
                     /translation="MDTTSGRTNNNRGRIRRLVRKAVYALLAIAAAAWGAVLLMPSPV
                     PVKVMEVAAGPFSVTVSEEGKTAIRNRYTVSAPVTGYLNRTVLRAGDRVRRGETVLAV
                     LDGEPSGLLSPRALAEAEARLRAAEAARSLRKAELDRARVGYDLAKKQFERIDRLLPS
                     GGIAKQDWDVAENRMQLLSRDVKAAEFALRMAEFDAEQARSSLIQGPEKGKPSEPVRV
                     LSPVDGYVLKVFEESARPVQAGTPLLEVGDPDDLEGEADLFSADAAGVVPGMEASIDG
                     WGGDRSLRGRVSAVERSGFTKVSALGVEEQRVKVRVDILDPVPPGYLLGDRFRIVVRI
                     VVRSDG"
BASE COUNT          171 a          410 c          328 g          191 t
ORIGIN      
        1 gccatccgac cgcaccacga tgcgcaccac gatgcggaac cggtctccca gcaggtagcc
       61 gggaggaacg ggatccagta tgtccacccg caccttcacc cgctgctcct ccacgccgag
      121 ggcggacacc ttggtgaagc cgctgcgctc cactgcggac acccggcctc tcagggacct
      181 gtctcccccc cagccgtcga tggaagcctc cattccgggc accaccccgg cggcatcggc
      241 ggagaacagg tccgcctcgc cctccaggtc atccgggtcc cccacctcga ggaggggcgt
      301 tccggcctgc accggcctgg cactctcctc gaagaccttc agcacgtacc cgtccacggg
      361 agagaggacc cgcaccggtt cggagggttt ccccttttcc ggaccctgta tgagggagga
      421 ccgggcctgc tccgcgtcga actccgccat gcggagggcg aactccgccg ccttcacatc
      481 cctggagaga agctgcatcc tgttttccgc cacgtcccag tcctgcttcg caatgccccc
      541 ggagggcagg aggcggtcta tccgctcgaa ctgttttttt gccaggtcat agcccaccct
      601 cgcccggtca agctcggcct tccgcaggga ccgggccgcc tccgccgccc gaaggcgggc
      661 ctccgcctcg gcgagggccc tgggggagag gagccccgac ggttcgccat cgaggacagc
      721 cagaacggtt tccccccgcc ggacccggtc gcccgcccgg aggaccgtcc tgttcagata
      781 tcccgtcacg ggggcggaca cggtgtaccg gttccggatg gccgtctttc cttcttccga
      841 gacagtgacg gagaagggtc cggcggcaac ttccatgacc ttcaccggaa cgggggaggg
      901 catcagcaga acggctcccc aggccgcggc ggctatggcg aggagggcgt acaccgcctt
      961 cctgaccagc ctccggattc ttcccctgtt gttgttggtc cgtcccgaag tcgtgtccat
     1021 gcaccttacc gtcctttctt cattctcctg atttcagcac gccgagcagg tccagggagg
     1081 ctattcttct cgtcaccacg
//
LOCUS       DAOTHD010000900         1100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_160021_length_1100_cov_2.110048, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000900 DAOTHD010000000
VERSION     DAOTHD010000900.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160021_length_1100_cov_2.110048"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="P5219_12315"
     CDS             <1..198
                     /locus_tag="P5219_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HRX27598.1"
                     /translation="NQFAMVPMWFMLVTTVAALGIMIKDNLAYASPNYVLVVPSIILL
                     VLALLMVFESMKALKQAQKDL"
     gene            complement(291..467)
                     /locus_tag="P5219_12320"
     CDS             complement(291..467)
                     /locus_tag="P5219_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression protein HypA/HybF"
                     /protein_id="HRX27599.1"
                     /translation="MGKFRCLSCKSEFELEEKAAAMRCPRCGSRFVEVLDAANRKRGK
                     SWSAKSFSVGKSGK"
     gene            633..>1100
                     /locus_tag="P5219_12325"
     CDS             633..>1100
                     /locus_tag="P5219_12325"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRX27600.1"
                     /translation="MLFSSMKDSRDVCCVLCGAAGLGIQTVEDMLAGIIADEGFSVFG
                     SREYMSRVRGGNNSTELRIASEPVDALIDRIDILVALSRKVRRNILERISSDTVIFGD
                     REELKGELEDLGASFVDIPLSSMAKEAGGRIYSNSIAAGVLLGVLGLGTKGAED"
BASE COUNT          222 a          305 c          315 g          258 t
ORIGIN      
        1 aaccagttcg ccatggttcc catgtggttc atgcttgtga ccaccgtggc ggccctgggc
       61 atcatgatca aggacaacct cgcctacgcc agcccgaact acgtcctcgt ggttccttcc
      121 atcattctcc ttgtccttgc cctgttgatg gtctttgaat ccatgaaggc cctgaagcag
      181 gcccagaagg atctgtaaag gtcagaccgg cagaaaagag agggaaggcc gttattcggc
      241 cttccctctt ctgtattccg aaaaagtgtt tctgcggtgc atacgtacgg ttatttgccg
      301 ctttttccca cgctgaacga cttggcgctc caggacttcc cccgcttcct gttggcggcg
      361 tcgagcactt ccacgaatct gctcccgcac ctggggcatc tcatggctgc cgccttttct
      421 tccagttcaa attcactttt gcacgacaga catcgaaatt ttcccatgga aaatcacctc
      481 cgcatatttt tccggtattg attcaatact ggggtatcgg caaaagaatt tgtttcatat
      541 tataccagaa cgccccggtt ggtgaaggaa tccgcagcgg gctgcggcag agggtataat
      601 aggtccagct tgaaactgaa gggaggacga acatgctctt ttcatcgatg aaggacagtc
      661 gggacgtctg ctgcgtcctg tgcggagccg cggggctggg catccagacc gtggaggata
      721 tgctggcggg aatcatagcg gacgaaggtt tttccgtctt cggatcccgg gagtacatgt
      781 cccgtgtccg gggggggaac aactccacag agctccggat agcgtccgag cctgtggatg
      841 ccctgatcga caggatcgat attctcgttg ccctgagccg gaaggtgcgc aggaacatct
      901 tggagcggat ctcctccgac acggtcattt tcggcgacag ggaagaactg aagggggagc
      961 ttgaagacct cggggcttcc ttcgtggaca ttcccctgtc gtccatggcc aaggaagccg
     1021 gcggcaggat ctactccaat tccatcgcgg ccggcgtcct cctcggggtg ctcggcctgg
     1081 gaacgaaggg tgcggaggac
//
LOCUS       DAOTHD010000901         1099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_160218_length_1099_cov_2.376437, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000901 DAOTHD010000000
VERSION     DAOTHD010000901.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160218_length_1099_cov_2.376437"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="P5219_12330"
     CDS             complement(<1..457)
                     /locus_tag="P5219_12330"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRX27601.1"
                     /translation="MLPALDEEVIRHRIAKRIALDFEDGAVVNLGIGIPTLVSDYLPE
                     DVHVLLQTENGVVGAGPKPEKEDLRFIGAGGRCVSLLPGSSLVASDMSFGLIRGGHLD
                     ATVLGALEVDSSGNLANWMIPGKMVPGMGGAMDLVTGARAVYVATTHCDK"
     gene            complement(441..1094)
                     /locus_tag="P5219_12335"
     CDS             complement(441..1094)
                     /locus_tag="P5219_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HRX27602.1"
                     /translation="MKPGSSLMVGGFNYAGVPYTLIDALVVQGTKDLHLISNDTLYAN
                     DQHPEGIGHGKLVVNGQCRKVTASHVGLNRETQRLFNEGKMELELVPQGSFVERIRCG
                     GFGLGGVLTPTGVGTIHEEGKQVIEVDGKKYILEIPLKADVALIRGHKADRWGNLTYF
                     GTGRNFNPAMATAADLVIAEVDTIVAEGEIDANDVVTPGMLVDILVLKGDGYYASRT"
BASE COUNT          172 a          349 c          343 g          235 t
ORIGIN      
        1 tcttgtcgca gtgggtggtg gccacgtaga cggcccgggc tcccgtgacg aggtccatgg
       61 cgcctcccat gcccggaacc atcttgccgg ggatcatcca gttcgccagg ttgccggagc
      121 tgtccacctc cagggcgccg aggaccgtgg cgtcgaggtg tccgccccgg atgaggccga
      181 agctcatgtc gctcgccacc aggcttgatc ccggcagcag ggacacgcac cgtcctccgg
      241 cgccgatgaa cctcaggtcc tctttttcag gcttgggacc cgccccgacc acgccgtttt
      301 ccgtctggag cagcacgtgc acgtcctcgg gaaggtagtc ggataccagg gtgggaatgc
      361 ctattccgag gttcaccacg gcaccgtcct cgaaatcgag ggcgatccgc ttcgctatcc
      421 tgtggcggat gacttcttca tcaagtgcgg gaagcatagt atccgtctcc tttcagtacg
      481 agaatatcca cgagcatgcc gggggtgacc acgtcgttcg cgtcgatttc cccctccgcc
      541 actatggtgt ccacttcggc gatgaccagg tccgcggcgg tcgccatggc ggggttgaaa
      601 ttccggcccg tgccgaagta ggtgaggttg ccccaccggt cggccttgtg cccccggatc
      661 agggccacgt cggccttgag ggggatctcg aggatgtatt tctttccgtc gacttcgatg
      721 acctgcttgc cttcctcgtg gatggtcccc accccggtgg gggtgagaac gcctcccagg
      781 ccgaagccgc cgcagcggat cctctcgacg aagctcccct ggggaacgag ttcgagttcc
      841 atctttccct cgttgaaaag tctctgggtc tcccggttca gtccgacgtg ggatgcggtg
      901 actttccggc actggccgtt caccaccagc ttcccgtggc cgattccctc agggtgctgg
      961 tcgttggcgt agagggtgtc gttggagatg agatggagat ctttcgttcc ctggaccacg
     1021 agggcgtcga tcagggtata gggaaccccc gcatagttga atcctcccac catcagggag
     1081 gagccgggtt tcacgttct
//
LOCUS       DAOTHD010000902         1098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_160546_length_1098_cov_1.627037, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000902 DAOTHD010000000
VERSION     DAOTHD010000902.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160546_length_1098_cov_1.627037"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..>1098
                     /locus_tag="P5219_12340"
     CDS             111..>1098
                     /locus_tag="P5219_12340"
                     /inference="COORDINATES: protein motif:HMM:NF014381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HRX27603.1"
                     /translation="MKRIAVLGGGNGAQALAGHLASKGFPVTLFEHPEFRKSIRHLED
                     RKTIELTGAIEAKGTLEEVTTDPAAVRGAEILYFLAPSFAQEAVFSLIAPYLGRGQRL
                     LLMPGNFGSLVLKETFGGRLPEGFLAAESDTMPYACRIEAPGVVNVWGVKNFMATAAL
                     PASGTGDFIASVQDAFPVPLKPAPNVLSIGLSNTNMILHCPTMIMNAGRIESEDGRFR
                     FYAEGMTGSVCAVMEAMDRERMAVGKALGLDLLSTMDDMKELYSINGESLRETILNNG
                     AYCGHGADSPTSMKYRYLTEDVPYLLVPVFQIGKKLGIPQPVTESIIRLASVI"
BASE COUNT          219 a          323 c          340 g          216 t
ORIGIN      
        1 cacctccaca tgatatgatc ctttccgtaa tacggtcaga ggattataat tcaagttcat
       61 tgtgatgtct acaatgtatt ttcgtttata ttcgggagga gacaggctca atgaaaagaa
      121 tcgctgttct ggggggaggc aacggggccc aggccctggc ggggcatctg gcgtcgaagg
      181 gctttccggt gacgctattc gagcaccccg aattccggaa aagcatccgc catctggagg
      241 ataggaagac catcgagctg acgggggcga tcgaggcaaa gggaacgctg gaggaggtca
      301 ccacggaccc ggcggcggta cggggagcgg agattctcta ttttcttgcg ccatccttcg
      361 cccaggaggc cgtgttctcc ctgatcgccc cttacctggg gcgggggcag agattactgc
      421 tcatgccggg gaatttcggg tccctcgtac tgaaggaaac cttcggaggc cggctgccgg
      481 aggggtttct tgcggcggaa tcggacacca tgccctacgc ctgccgcatc gaggcccccg
      541 gggtggtgaa cgtctgggga gtaaagaact ttatggcgac ggcggccctg cctgcttccg
      601 ggaccgggga tttcatcgcc tcggtgcagg acgcctttcc cgtgcccctg aagccagccc
      661 cgaacgtgct gtccatcgga ctctccaaca ccaacatgat cctccactgc cccaccatga
      721 tcatgaacgc cgggaggatc gagtcggagg acgggaggtt ccggttttac gccgagggca
      781 tgaccggctc cgtctgtgcc gtcatggagg ccatggacag ggagcggatg gccgtgggga
      841 aggctcttgg cctcgacctc ctctccacca tggacgacat gaaggagctc tactccatca
      901 acggggaaag cctgcgggag accatcctca ataacggggc ctactgcggc cacggagccg
      961 actccccgac gtccatgaaa taccggtacc tcaccgagga cgttccctat cttctcgtcc
     1021 ccgtattcca gatcgggaag aagctgggga tccctcagcc cgtgacggag agcatcatca
     1081 ggctggcttc cgtcatcg
//
LOCUS       DAOTHD010000903         1091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_161964_length_1091_cov_4.140927, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000903 DAOTHD010000000
VERSION     DAOTHD010000903.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161964_length_1091_cov_4.140927"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..>1091
                     /locus_tag="P5219_12345"
     CDS             49..>1091
                     /locus_tag="P5219_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HRX27604.1"
                     /translation="MGRVAESTTIMSVFKKMLNYSDIGMAGLVVLIVVMMIIPLPTWL
                     LDVLLTLNITIGVVVLLSTFYAQRALDLAAFPTMLLIVTLFRLALNVSTTRLILLNAD
                     AGQVISAFGNFVVGGNYVVGAVVFLILVIIQFVVITKGSERVAEVAARFTLDAMPGKQ
                     MAIDADLNAGLIDEGGARERRLTIQREADFYGAMDGASKFVKGDAIAGLIITIINIIG
                     GLSIGVLQRGLPLEKALGLYSLLTVGDGLVSQVPALLFSTATGIIVTRAAGQTDLGRD
                     IFTSLTNYPRPLFIGAVLLFALAVVPGLPTIPFLLLACFLTLMGYWVYREGKIQEAGG
                     GEKRREGGKTQAPG"
BASE COUNT          200 a          334 c          329 g          228 t
ORIGIN      
        1 ggttttcatg tagaatagaa cgatacagac tgggttatca agggaaggat gggtcgggtg
       61 gcggaatcga cgacgatcat gtcggtcttc aaaaaaatgc tcaattattc ggatataggc
      121 atggcggggc tcgtggtcct catcgttgtg atgatgatca tcccgctgcc cacgtggctt
      181 ctggacgtgc tcctgacgct gaacatcacc atcggagtgg tggtcctgct ttcgacgttc
      241 tatgcccaga gagccctcga tctcgcagcg ttccctacca tgctcctgat cgtgaccctc
      301 ttccgtctcg ccctcaacgt gtccaccacg aggctcatcc tgctgaacgc cgatgcgggg
      361 caggtcatca gcgccttcgg caatttcgtg gtgggaggaa actacgtcgt cggcgcggtg
      421 gtctttctca ttctcgtcat catccagttc gtcgtcatca ccaagggatc ggaacgtgtg
      481 gcggaagtgg ctgcgcggtt cactcttgac gccatgcccg gaaaacagat ggccatcgac
      541 gccgacctga acgccgggct catcgacgag gggggcgcca gggaacggcg gctcaccatc
      601 cagcgggagg ccgacttcta cggggccatg gacggcgcgt ccaagttcgt caagggggac
      661 gccatcgccg gtcttatcat caccatcatc aacatcatcg gcgggctgtc catcggggtg
      721 ctccagcggg gccttcccct ggaaaaggcc ctggggctgt acagcctcct gacggtgggc
      781 gacggcctcg tatcccaggt tcccgcactg ctgttctcaa ccgccaccgg catcatcgtc
      841 accagagcgg cggggcagac ggacctgggc cgggatattt tcacatccct gaccaattat
      901 ccccgccccc tcttcatcgg ggccgtgctg ctctttgccc tggccgtggt gcccgggctg
      961 cccaccatcc ccttccttct tctcgcctgt ttcctgaccc tcatgggcta ctgggtctac
     1021 agggaaggaa aaattcagga agcgggagga ggggagaagc gccgtgaagg agggaaaacc
     1081 caggcgccgg g
//
LOCUS       DAOTHD010000904         1091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_161989_length_1091_cov_3.192085, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000904 DAOTHD010000000
VERSION     DAOTHD010000904.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161989_length_1091_cov_3.192085"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1091)
                     /locus_tag="P5219_12350"
     CDS             complement(<1..>1091)
                     /locus_tag="P5219_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27605.1"
                     /translation="SLFLMTYPYGCLEQTVSGAWPLLVLPELAADMDPALVNRDEIDA
                     ALALRIRQIGAMQLYHGAFASWPGSSSAYPWGSVYAAHFLVEAQKSGIPVPEDLAGGA
                     LSYVRALLPLMPDDESEWRMRENMTLKAYASYVLALAGEPPLGWMAHISEHKNLLRGS
                     GSVFLAGAYALSSKTSEPLKELGTSAPSLREGDITTLESASRTDALKLLMWTEVDPLS
                     APAAELAGKLIGEARKKAWGTTQDNAMSVLALGRWIERTKEARKPFTAILRDQSGAQV
                     ASFSDGRRMSLELKDLPEGQLSLELTGEGTAYFAWTAAGVPEKAPAPGSRGVTVSRTW
                     ADRKGNPIPQGTPVERGDRIEVTLTITPS"
BASE COUNT          181 a          327 c          364 g          219 t
ORIGIN      
        1 ccgacggagt gatggtaagg gtgacttcga tgcggtcgcc ccgctccacg ggggttccct
       61 gcgggattgg gttccctttc ctgtctgccc atgtccggct cacggtgact ccccgcgagc
      121 ccggggccgg ggccttttcc ggcacgcctg ccgcggtcca ggcgaaatag gcggtcccct
      181 ccccggtcag ttcaagggag agctgacctt cgggaagatc tttcagttcc agggacattc
      241 tcctgccgtc ggagaaggag gcgacctgtg ctcccgactg atccctgagt attgcggtga
      301 aaggtttccg ggcctctttc gtgcgctcga tccaccgccc cagggcaagg acgctcatgg
      361 cgttgtcctg ggtcgtgccc caggccttct ttctcgcctc gccgatgagc ttcccggcga
      421 gttcggccgc aggggcggac aggggatcca cctcggtcca catgagcagt ttcagggcgt
      481 cggtccttga ggcggattcc agggtggtga tgtctccttc ccggagggaa ggcgccgaag
      541 tcccaagttc cttcagaggt tcggaggtct tcgacgagag ggcgtaggcg ccggcgagga
      601 acaccgagcc cgaccccctg agaaggttct tgtgttcgct gatgtgggcc atccacccga
      661 ggggaggttc tcccgcaagg gcaaggacat aggaagcgta ggccttcagc gtcatgtttt
      721 ccctcattct ccattcgctt tcatcgtcag gcatcagggg caggagggcc ctgacgtaag
      781 agagggctcc tccggcaagg tcttctggaa ccggtattcc gctcttttgt gcctccacga
      841 ggaagtgggc ggcgtagacg gatccccacg ggtaggccga cgagcttccc ggccatgaag
      901 cgaaggctcc atggtagagc tgcatggcgc cgatctgccg tatcctgagg gccagggcgg
      961 cgtcaatttc gtccctgttc acaagggcgg gatccatgtc ggccgccagt tccggaagga
     1021 cgagaagggg ccatgctccg gaaacggtct gttccaggca cccgtaagga taggtcatga
     1081 ggaacaggga g
//
LOCUS       DAOTHD010000905         1091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_162042_length_1091_cov_2.420849, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000905 DAOTHD010000000
VERSION     DAOTHD010000905.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162042_length_1091_cov_2.420849"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..>1091)
                     /locus_tag="P5219_12355"
     CDS             complement(160..>1091)
                     /locus_tag="P5219_12355"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRX27606.1"
                     /translation="SVLTTVLAVLHPSGFAWSIAVWLWFTVLFANLAEAVAESRGRAQ
                     AQTLRRTRTETTARRLTADGREERVPGTYSDRASGNMFLFQLMGVDSIRVVPAGADMT
                     AEMEKTAAELRASGRKPYIIPGGGSNAVGALGYVACAEEILSQSFRKGVSFDRVFTTS
                     GSSGTHAGLAVGLWANRSGIPLAGINISRPNSLQVPLVEKVARETAALLGIEEPLPGG
                     LIQCFDGYVGEGYSLPTPAMTKTVVLLSRMESILLDPVYTGKAFAGMLDLIGQGFCKK
                     GERVLFLHTGGVPALFHYQKYFDPELFPEDGRR"
BASE COUNT          200 a          341 c          371 g          179 t
ORIGIN      
        1 ggacggagcc gccgacggca cggtgttgct caccgccgcg ccgagaagca gggcaatgcc
       61 caccgcccag gttttcgtgg cgggcaggag ggccagagcc accacgacgg cgagaaagag
      121 aagaaacggg acgttcgttc gtttcagttc caaaagtgac tacctccgcc cgtcctccgg
      181 aaacagttcc ggatcgaaat acttctggta gtggaagagg gcgggcactc cccctgtgtg
      241 gaggaagaga acccgttccc cctttttgca gaatccctgc ccgataaggt caagcattcc
      301 ggcgaaggcc tttccggtat acacgggatc gagcaggatg gattccatcc gggacagcag
      361 gaccaccgtc ttcgtcatgg cgggggtagg cagggaatac ccttcgccca cgtagccgtc
      421 gaagcactgg atgaggccgc cggggagagg ttcttcgatc ccgagaaggg cggccgtctc
      481 gcgggcgacc ttctccacga gaggaacctg cagcgagttc ggacggctga tgttgattcc
      541 cgcgagggga attcccgaac ggtttgccca gagtcccacg gccaggcccg catgggtgcc
      601 ggagctgccg ctggtggtaa agaccctgtc gaaggaaacg cccttccgga aagactggga
      661 gagaatctcc tcggcgcagg ccacgtagcc cagggctccc acggcgtttg atccgccgcc
      721 gggaatgatg tagggtttcc tgcccgacgc cctgagttcc gccgctgtct tttccatttc
      781 ggcggtcatg tcggctcccg cgggaaccac ccggatggag tccactccca tgagctggaa
      841 gaggaacatg tttcccgatg ctctgtcgga ataggtgccg gggacccgct cctcccgccc
      901 gtcggcggtg aggcgccgcg ccgtcgtctc ggtgcgggtg cggcgcagcg tctgcgcctg
      961 ggcgcggccg cggctctcgg cgacggcctc ggcgaggttg gcgaacagca cggtgaacca
     1021 cagccagacg gcgatcgacc aggcgaaccc gctcgggtgc aggaccgcga gcacggtggt
     1081 gaggaccgag c
//
LOCUS       DAOTHD010000906         1090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_162263_length_1090_cov_2.547826, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000906 DAOTHD010000000
VERSION     DAOTHD010000906.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162263_length_1090_cov_2.547826"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="P5219_12360"
     CDS             complement(<1..732)
                     /locus_tag="P5219_12360"
                     /inference="COORDINATES: protein motif:HMM:NF014198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HRX27607.1"
                     /translation="MDRKIGLADLSPRCWTDALEQVRRQRPLVYTITSFVSASFQADG
                     TIAAGGSPVMSRCPREAAELAASADSIVINTGTPDESALLAMNRAVKTGKKIVFDPVG
                     YGATAFRTAAVNSLLSACRPSVIKGNYGEIRLLAGQAGEMRGVDSECATAPLVPVMQS
                     LAGRTGALICATGPVDIIADGASARAFSGGSALMARISGGGCLLGSLMGVLISGGIAA
                     GASAAVVMLRLAAERAEERAKGPGAF"
     gene            complement(717..>1090)
                     /locus_tag="P5219_12365"
     CDS             complement(717..>1090)
                     /locus_tag="P5219_12365"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HRX27608.1"
                     /translation="LSGMTIESVEDMEKAAEAIGRTGPGAVLVKGGHRMQADSVTDVF
                     YCRGHLQRFTDSRIHTENTHGTGCTLSAAIAAELASGSSLEEAVRRGRQYLRLALKSG
                     FRPGTGWGPLGHAVIPPWTEK"
BASE COUNT          193 a          362 c          337 g          198 t
ORIGIN      
        1 gaaggccccc ggccctttcg ccctttcttc agcccgctcc gccgcgaggc ggagcatcac
       61 aacggccgca gaagctcctg cggcgattcc tccggaaatg agcaccccca tgagggatcc
      121 gagaaggcag cctcctccgg aaatcctggc catgagagcg ctccctccgg aaaaggcccg
      181 ggcagacgct ccatcggcaa tgatatccac ggggccggtg gcgcagatga gggctccggt
      241 ccgccccgcg agggactgca tgaccggaac aaggggggcc gtcgcacatt ctgaatccac
      301 tcccctcatc tccccggcct gaccggcgag cagccggatc tctccgtagt ttcccttgat
      361 gacggaaggg cggcaggccg aaagaagact gttgacggct gctgttctga aggcggtcgc
      421 tccgtagccc accgggtcga acacgatttt tttccctgtc ttcacggccc ggttcatggc
      481 caggagggcg ctctcgtcag gggttcccgt gttgatcacg atggagtccg ccgacgccgc
      541 aagctccgcc gcctcccggg gacagcggga cattaccggg gagcctcctg cggcaatggt
      601 tccgtccgcc tggaaagatg ccgaaacgaa gctggtgatg gtgtaaacca gggggcgctg
      661 ccgccgcacc tgttccaggg cgtctgtcca gcaccttggg gaaagatctg cgagtcctat
      721 ttttctgtcc acggagggat caccgcgtgt ccgagaggcc cccaccccgt gccggggcga
      781 aagccggatt tcagggcaag gcggagatac tgccgtcccc ggcggaccgc ttcttcgagg
      841 gaggaaccgg aagccagctc cgcagctata gctgcgctca gggtgcatcc cgtaccgtgg
      901 gtgttttccg tatggattcg ggagtcggtg aaccgctgca ggtgtccgcg gcaatagaag
      961 acatccgtga ctgaatcggc ctgcatcctg tggcctccct tcacgagaac cgcaccggga
     1021 cctgttctcc cgatggcttc cgccgccttt tccatatcct ccaccgactc tatggtcatg
     1081 cccgaaagcc
//
LOCUS       DAOTHD010000907         1089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_162582_length_1089_cov_2.096712, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000907 DAOTHD010000000
VERSION     DAOTHD010000907.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162582_length_1089_cov_2.096712"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="P5219_12370"
     CDS             complement(<1..713)
                     /locus_tag="P5219_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRX27609.1"
                     /translation="MSLMTTRQGGESPGKKSVYLRLLSSARPYRKRFFWAVGCMLLAS
                     ACNVVPPWLLKNVVDDVLIARNLAMLNVLPFLLVALFAGKGIASYGHQYLMNWVGQNV
                     VMDLRLRLYDHLQRMSLRYLHGNRLGEIMSRITNDVTILQNLVTSVVVNLVVQSVTFL
                     GMVGFLFYINWKLTLITFAILPLTVLILDRASKKLRIVGHAIQSELAGLSAVVQEAFS
                     AVRVVRSFATEDLELSRFR"
     gene            complement(710..>1089)
                     /locus_tag="P5219_12375"
     CDS             complement(710..>1089)
                     /locus_tag="P5219_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27610.1"
                     /translation="LMHRGIPFCVLVPFASLRKVPLPGLAGLIASLPGIGPRLRETVL
                     RRRRRKMELLAWPNRLAGRMLADECIGEYSPESAAEFVVSWLHDRSRRDGLREALGDV
                     AGSAPQGAAVRIADEIERMTVRV"
BASE COUNT          217 a          334 c          356 g          182 t
ORIGIN      
        1 ctgcggaaac gggaaagctc caggtcctcc gtggcgaagg accggacgac ccggaccgcc
       61 gaaaaggcct cctgcaccac cgccgacagc cccgcaagct cgctctggat ggcgtgcccc
      121 acgatccgga gcttcttcga cgcccggtcg aggatcagca ccgtgagagg gaggatggcg
      181 aaggtgatga gggtaagctt ccagttgatg tagaagagaa atcccaccat gccgaggaag
      241 gtgacggact ggaccaccag gttgaccacc acggaggtga ccaggttctg gaggatggtc
      301 acgtcgttgg tgatccggga catgatctcc ccgaggcggt tcccgtggag gtacctgagg
      361 gacattctct ggaggtggtc gtacagccgc aggcggaggt ccatgaccac gttctggccc
      421 acccagttca tcaggtactg gtggccgtag gaggcgatgc ccttcccggc gaagagggcc
      481 accaggagga agggaaggac gttgagcatg gccagattcc gggcgatgag gacatcgtcc
      541 accacgtttt tgaggagcca gggaggaacc acgttgcacg ccgaggcgag gagcatgcac
      601 cccaccgccc agaaaaaccg tttccggtag gggcgggcgc tggaaaggag ccgaaggtag
      661 accgatttct ttcccgggct ttcgccgccc tgccgcgttg tcatcaggct catacccgga
      721 cagtcatcct ttctatttcg tcggcgatcc ggacagcagc tccctggggg gccgacccgg
      781 caacgtctcc gagggcttcc cggaggccgt ctcttcgtga ccggtcgtgg agccatgaaa
      841 ccacgaattc tgccgccgat tcgggggaat actccccgat gcattcgtcc gccagcatcc
      901 ttccggcaag tcggttcggc caggccagaa gctccatttt cctcctcctg cgcctgagaa
      961 ccgtttcccg aagcctcggc ccgatccccg ggagggacgc gatcagcccg gcaagccccg
     1021 ggaggggcac cttccgtagg gaggcgaagg gaacaagcac gcagaagggg attcccctgt
     1081 gcatgagtt
//
LOCUS       DAOTHD010000908         1087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_162935_length_1087_cov_2.781977, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000908 DAOTHD010000000
VERSION     DAOTHD010000908.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162935_length_1087_cov_2.781977"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="P5219_12380"
     CDS             complement(<1..1064)
                     /locus_tag="P5219_12380"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF036802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRX27611.1"
                     /translation="MALLLSDSTNVERPGFTQSEHTLSGTIDRLFRQHRSRRIVISAF
                     ASNLHRVQQVVDAAARFNRKVALVGRSMVKNVELARELGYLHADDKTIVDIEDIGKVP
                     PGSLVVMTTGSQGEPFSGLVLMSRGEHRVITLTEKDVVAIFATPIPGNEKTVSNTINR
                     LFACGCEVVYEKEQAIHVSGHAAQDELRVMLNMVRPQFFVPVHGEYRHLVRHAKLARE
                     MGVAPKNAFVLLNGDVLTLEKDKAVVKDRVQAGGIMVDGIALGEMQGSVLKERRELAE
                     DGAVAVSLILTGEGTLASPPVFESRGFLHLEDAAKLREDLTVSVGKAVESLGAKAVSD
                     RDLLETRIKGRMRDALRRFGR"
BASE COUNT          183 a          363 c          337 g          204 t
ORIGIN      
        1 cgtccgaacc gccggagggc gtcccgcatt ctgcccttta tccgcgtttc gagaaggtcc
       61 cggtcggaaa cagcctttgc tccgagggat tccacggcct tcccgacgga gaccgtgagg
      121 tcttccctca gcttcgccgc atcctcaagg tggagaaacc ctctgctttc aaaaaccggg
      181 ggcgaggcga gggtcccctc gcccgtcagg atgagggaga cggccacggc cccgtcctcg
      241 gcgagttccc tccgctcctt cagcacgctc ccctgcattt cgccgagggc gatgccgtcc
      301 accatgatgc cgccggcctg tacccggtcc ttcaccacgg ccttgtcctt ttccagggtc
      361 agcacgtcgc cgttcagcag gacaaaagcg tttttcggag ccacgcccat ctcacgggcg
      421 agcttcgcgt ggcggacgag atggcggtat tcgccgtgga cgggaacgaa gaactggggc
      481 cggaccatgt tcagcatgac ccgaagctcg tcctgtgcgg cgtggcccga gacgtggatg
      541 gcctgctcct tttcgtagac cacttcgcaa ccgcaggcaa acaggcggtt gatggtgttg
      601 ctcaccgttt tttcgtttcc gggaatggga gtggcgaaga tggccaccac gtccttctcc
      661 gtcagggtga tcacccggtg ctcccccctg ctcatgagga caagaccgga gaagggctct
      721 ccctggctcc cggtggtcat gaccaccagc gaacccggcg gaaccttgcc tatatcctcg
      781 atgtccacga tggtcttgtc gtcggcgtga aggtagccca actcccgggc gagctccacg
      841 ttcttcacca tgctccgccc caccagggcg accttccggt tgaacctggc cgccgcgtcc
      901 accacctgct gcacccggtg gaggttgctg gcgaaggcgg aaatgacgat gcgcctcgac
      961 cggtgctgcc ggaaaagccg gtcgatggtg ccggacaggg tgtgctccga ctgggtaaag
     1021 ccggggcgct ccacgttggt ggagtcggag agcagcagcg ccacgccacg gtcaccaagg
     1081 cgtgaca
//
LOCUS       DAOTHD010000909         1086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_163118_length_1086_cov_3.117362, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000909 DAOTHD010000000
VERSION     DAOTHD010000909.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163118_length_1086_cov_3.117362"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1086)
                     /locus_tag="P5219_12385"
     CDS             complement(<1..>1086)
                     /locus_tag="P5219_12385"
                     /inference="COORDINATES: protein motif:HMM:NF023478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HRX27612.1"
                     /translation="TSCGWFFDELSGIETLQVMAYAARALELGERLFPARDFRTPFRR
                     KLAEAQSNIAEFRDGRRIFDIFVEPLRADLLRAGAHFAVSCLFVPGEEKACSPERCAF
                     SSYRISGCSLERKESGNNRYALGYLRIMSETTFEEDDLFVAALYRGGRDVLCGVARKA
                     APAGGNGVRERMEAALRDEDEQALVDFFGHNVYSLRHLFKDEQRRILNRIISEDVASV
                     TDTLRETVRDYSEVLSFLAALSMPAPDAFRSAAEVVLNDDLRKALESLPLDLVFLERR
                     VADAETWGISLDLEGLRHTAAGRLEEFLGRLGDEPENRAILGELHALLSFLARRKWDV
                     NLWEVQNRFAKILNGKRELSQDSWELYS"
BASE COUNT          220 a          339 c          318 g          209 t
ORIGIN      
        1 gaatagagct cccaactatc ctgagaaagc tcccgttttc cgttcagtat ttttgcaaac
       61 cgattctgca cttcccagag gttcacatcc cacttcctcc gtgcaagaaa tgagagcagg
      121 gcatgaagtt ccccgaggat ggccctgttc tccggctcat ccccgagccg gccgaggaat
      181 tcctcgagac gcccggctgc cgtgtgacgc aggccctcaa ggtcaaggga gattccccag
      241 gtttcggcgt cggcaaccct ccgctccagg aaaaccaggt cgaggggaag gctttcaagg
      301 gccttccgca ggtcgtcgtt gagcaccacc tcagcggcac tccggaaggc gtccggtgcg
      361 ggcatggaga gggcggcgag gaaggacaga acttcggaat aatccctgac cgtctcccgg
      421 agcgtgtccg tgacggatgc cacgtcctcc gaaatgatac ggttgaggat cctccgctgc
      481 tcatccttga aaaggtgccg gagagaatat acattgtggc cgaagaaatc gaccagggcc
      541 tgttcgtcct cgtccctcag ggccgcctcc attctttccc gcactccgtt cccgcccgcc
      601 ggggcggctt tccgggccac cccgcacaga acatcccttc ctccccggta gagtgccgcc
      661 acgaagagat cgtcctcctc gaaggtggtt tcggacatga tcctgaggta cccaagggca
      721 taccggttgt ttccgctttc tttccgctcg agggaacacc cggaaatccg gtaggatgaa
      781 aaggcgcacc gttcagggga acaggctttc tcctccccgg ggacaaaaag gcaggaaacg
      841 gcgaaatggg cccctgcccg aagcaggtcc gcccggaggg gttcgacaaa gatgtcgaaa
      901 attctccggc cgtcccggaa ctctgcgatg ttgctttgtg cctcggcgag cttcctcctg
      961 aaaggggtac ggaaatcccg ggccgggaaa agccgctctc cgagttcgag ggcccttgcg
     1021 gcgtaggcca tgacctggag cgtttctatg ccggaaagct cgtcgaagaa ccagccgcag
     1081 cttgtg
//
LOCUS       DAOTHD010000910         1086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_163140_length_1086_cov_2.775946, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000910 DAOTHD010000000
VERSION     DAOTHD010000910.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163140_length_1086_cov_2.775946"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..912)
                     /locus_tag="P5219_12390"
     CDS             complement(67..912)
                     /locus_tag="P5219_12390"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRX27613.1"
                     /translation="MRREENPRQLLQSVIRAFRIAEMLAEEGELGVTEIDGRLGIDKS
                     TAYRILATLREMGYVRQNPLNSKYSNTPKFSLLGGTAPDMQQIRERARPVLRSLSERV
                     GEAVNLGCLDGTDIVYVERIQCDEMIQVNLPVGRRMPAYCSALGKAILAFLSAETVKQ
                     LFSGTSFAPYTERTVKSLTMLLAELERIRSDRFARDLQESHPGLSCLAAPVFLASGKV
                     AAGISVSFPAFRHPDMEKAERTIVPALLDAAESISGELGYSGRNREHDHPGGQPVPER
                     KRQEE"
BASE COUNT          195 a          360 c          314 g          217 t
ORIGIN      
        1 gatcccgtcc gggaattccc gcctgttcca tcatttttga cactgcatcc agagtaactg
       61 ccatgttcat tcctcctgtc gttttctctc cgggaccggc tgtccgcccg gatggtcatg
      121 ttcccggttc cgtccgctgt atcccagttc cccgctgatg ctttccgcag cgtccagaag
      181 ggcggggacg atggttcttt cggctttctc catgtcggga tgccggaagg cgggaaaaga
      241 aacgctgatg cccgcggcta cctttcccga ggcaaggaag accggggcag caaggcagga
      301 cagtcccggg tgggattcct gcagatcccg ggcgaagcgg tccgaccgta tcctttcaag
      361 ttccgccagc agcatggtca gcgatttcac cgtccgctcc gtgtatggcg cgaaggaagt
      421 gcccgagaaa agctgtttca cggtttcggc ggacagaaat gcgaggatcg cctttccgag
      481 ggccgagcag taagcgggca ttctccggcc caccggaaga ttcacctgga tcatctcgtc
      541 gcactggatc ctttccacgt acacaatgtc ggtcccgtcg aggcagccga ggttcaccgc
      601 ctcgcccacc ctttcggaca gggagcgcag caccggccgg gcccgctcac ggatctgctg
      661 catatccgga gccgttcccc cgagaaggga gaacttcggc gtattggagt acttgctgtt
      721 cagggggttc tgccgtacat agcccatttc ccgcagggtg gcgagaatcc tgtaggccgt
      781 gctcttgtcg atgccgagcc gcccgtcgat ctccgtgacc ccgagttccc cctcttcggc
      841 cagcatctcg gcgatccgga acgccctgat gacggactgg agcagctgcc gcggattttc
      901 ttcccgcctc acgacggatc ccctcccgat ttcttcccca tccggccatc gggaagaaag
      961 gagacggcca gcggcgcgat catcgcaccc agggccaggc tcaggaaggc cgttccccat
     1021 ccggaaccgt ttcccgcctc caccagccgg ccgaaaacga caggtgaaat caccgtgcac
     1081 aggaac
//
LOCUS       DAOTHD010000911         1084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_163623_length_1084_cov_2.603499, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000911 DAOTHD010000000
VERSION     DAOTHD010000911.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163623_length_1084_cov_2.603499"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(539..>1084)
                     /locus_tag="P5219_12395"
     CDS             complement(539..>1084)
                     /locus_tag="P5219_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRX27614.1"
                     /translation="YKDRRIGTVGPVVKGYEWKLLNDKNEDVTASGEGVLWVKGPSVT
                     SGYFRDPVMTADRFVDGWFNTGDYVRMEDGFVRILDRVTDIIIVGGFNVYPQEVEAIL
                     HTHPAISQAVVVGIPHPVNGEIPKAFIRLQEGARVTPREIIDFCKKHLAHFKVPRSVE
                     FMEAFPLSSTGKVLRRLLRQK"
BASE COUNT          218 a          340 c          307 g          219 t
ORIGIN      
        1 cagggcggga agaacgttca gaaagctgtt ccaggccttg ttcagagcct tcttcgtctt
       61 ttccctgctt gccgccgccg acacggcgag ggccgccccc gccgcgaggt acaggaacca
      121 tgtcatggtg cagaaccacg tcctttcgaa aaactgatcc ggaagagatc catactgtac
      181 catatatatt tgctgaacgg actcccccgt tgtgccgcga tgccggacaa aggctgaaag
      241 cgctccgcag gaagggagca tttccgggta acgggcctgc agaaggcagg aggagcccca
      301 tccggctttc cgggatattc tctgttataa tacccccagg gggtgataaa ccatgagcga
      361 tgacgagaaa aaaagaaggg gtttcgcctg ccctccctga ctgctcgtgc tcatcgccgc
      421 ggcggtcgtc gccggcattt cccgtctgtt caggatgttc cggtaaaaag aaaaaagtcc
      481 cgctccggca agtccggaac gggactcatt tttctcccgc tctgcctccg aagggggcct
      541 atttctgccg cagcagcctc cggagaacct tccccgtgct tgacaggggg aaagcttcca
      601 tgaactcaac actcctgggg accttgaaat gggcgaggtg cttcttgcag aagtcgatga
      661 tctccctcgg cgtgacccgg gccccttcct ggagccggat gaaagccttg gggatctcgc
      721 cgttcaccgg gtgggggatg cccaccacca cggcctggga aatcgccggg tgggtgtgga
      781 gaatggcctc cacctcctgg ggatagacgt tgaaaccgcc gacgatgatg atgtccgtca
      841 cccggtcgag aatcctgaca aacccgtctt ccatcctgac gtagtccccc gtgttgaacc
      901 atccgtccac gaaccggtcc gccgtcatga cggggtccct gaaatagccg gatgtcacgg
      961 aaggcccctt cacccagagc accccttcgc ccgaggcagt cacatcctcg ttcttatcgt
     1021 tgaggagctt ccactcgtat cccttcacga cgggccccac ggtgccgatg cgccggtcct
     1081 tgta
//
LOCUS       DAOTHD010000912         1083 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_163894_length_1083_cov_2.378405, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000912 DAOTHD010000000
VERSION     DAOTHD010000912.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163894_length_1083_cov_2.378405"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..862
                     /gene="zupT"
                     /locus_tag="P5219_12400"
     CDS             47..862
                     /gene="zupT"
                     /locus_tag="P5219_12400"
                     /inference="COORDINATES: protein motif:HMM:NF003243.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HRX27615.1"
                     /translation="MEIENVLFAFGLTLFAGLSTGIGSALAFFTKRTNTRFLAFSLGL
                     SAGVMIYVSMVEIFFKAQEALQADLGERMGAWVTVVSFFLGILGVMLIDKFVPSHENP
                     HEMHRIEEIRGRAGELGQEHSLMRMGILTALAIAIHNFPEGIATFMAAIQEPRLGVPI
                     AVAIAVHNIPEGIAVSVPIYYATGSRRKAFRLSFLSGLAEPVGALLAYLVLMPFLSDT
                     LFGLIFAAVAGIMVFISVDQLLPSARKYGEHHVSIYGLIVGMMIMAVSLLLFI"
     gene            883..>1083
                     /locus_tag="P5219_12405"
     CDS             883..>1083
                     /locus_tag="P5219_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27616.1"
                     /translation="MKISFNLDQFLKDLEYLVNIDSQSKDTEGVAEVASFFEKAFAGI
                     GWKVEKKDFGPAVGPSLKITNGE"
BASE COUNT          207 a          337 c          307 g          232 t
ORIGIN      
        1 gtgtgtccgg gtacacgtga catcatatca aggaggtcgt cgtcacatgg aaattgagaa
       61 tgtacttttc gcattcggtc tcacgctgtt cgccggcctc tcgaccggaa tcggcagcgc
      121 gctcgcattc ttcacgaagc gcaccaacac ccgatttttg gcattctccc ttggtctctc
      181 cgccggggtg atgatctacg tctcgatggt ggagatcttc ttcaaggcgc aagaggcgct
      241 ccaggccgat ctgggagaga ggatgggcgc ctgggtcacc gtcgtctcct tcttcctcgg
      301 cattttggga gtgatgctga tcgacaagtt cgtcccctct cacgagaacc cgcacgagat
      361 gcaccggata gaggagatcc gcggccgtgc cggagagctg gggcaggagc actcgctgat
      421 gcgcatggga atcctcacgg cgctcgccat cgccatccac aacttccccg agggaatcgc
      481 caccttcatg gccgccatcc aggagccccg gctcggcgtt cccatcgccg tggccatcgc
      541 cgtccacaac atccccgagg gaatcgccgt ctccgtgccg atctattacg ccaccggaag
      601 caggagaaag gccttccggc tctccttcct ctccggcctg gcggaaccgg tgggggcgct
      661 gcttgcctat ctcgtcctca tgcccttcct gagcgatacc cttttcggcc tcatcttcgc
      721 cgccgtggcg gggatcatgg tcttcatctc cgtggaccag ctccttccct cggcgcggaa
      781 atacggggaa caccatgttt ccatctacgg gctcattgtg ggcatgatga tcatggccgt
      841 aagcctgctg ctttttatct gaccgaagga ggaaacagcc ccatgaagat ttcgttcaac
      901 ctggaccagt ttctgaagga cctcgagtac ctggtgaaca tcgacagcca gtccaaagac
      961 accgaaggag tggccgaggt ggcctcgttc tttgaaaaag ccttcgccgg catcggctgg
     1021 aaggtggaga agaaggattt cggccccgcg gtgggtccca gtctcaaaat aaccaacggc
     1081 gaa
//
LOCUS       DAOTHD010000913         1082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_164063_length_1082_cov_3.268744, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000913 DAOTHD010000000
VERSION     DAOTHD010000913.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164063_length_1082_cov_3.268744"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="P5219_12410"
     CDS             <1..208
                     /locus_tag="P5219_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter permease"
                     /protein_id="HRX27617.1"
                     /translation="YIMQKADMPSSPIILAVILGPMAESNLRRAVLMYEGSYSFLWTR
                     PIGVVFLALAALSLYSSYRRAHSS"
     gene            242..>1082
                     /locus_tag="P5219_12415"
     CDS             242..>1082
                     /locus_tag="P5219_12415"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HRX27618.1"
                     /translation="MKVVFVEKPHSLEIREVPVPGPGTGEALIRVRAGGICGSDMHIY
                     HGTNPLAKYPRIIGHEFSGEVAEVGKGVTGFVPGDRVAVDPVTSCGTCYPCSIGRPNV
                     CERLEVFGVHRDGGFAEYIVLPEKNLHRIPSDWSFEKGALVEPFTIAANVLSRTECSG
                     SDRLLILGAGPIGQVILQAARRLGTACAVADIVGVRLEKARSLGAELTVNTRETPLEE
                     TMAEWTGGAGVPLIIDAVGSPDLFPSLLRMASPAGRIGLLGFSKEPSSFVQLEAVKKE
                     LSIF"
BASE COUNT          201 a          333 c          338 g          210 t
ORIGIN      
        1 gtatataatg cagaaagcgg acatgccttc gtcgcccatt atcctggcgg tgatcctggg
       61 acccatggcg gaaagcaacc tccggcgtgc agtgctcatg tacgaaggga gctactcttt
      121 cctctggacc aggccgatcg gcgtggtgtt cctggccctt gcggcattgt ccctgtattc
      181 atcctaccgg cgggcccatt cctcgtagag gcgccgggag agaagaaaag gagaacggat
      241 catgaaggtc gtttttgtag agaagcccca cagtcttgaa atccgggagg ttcccgttcc
      301 cggcccggga acgggagaag cgctcatacg ggtccgtgcc ggcggcatct gcggctcgga
      361 catgcatatc taccacggca ccaaccccct ggcgaaatat ccgaggatca tcggacacga
      421 gttctccgga gaggttgctg aggttgggaa aggcgtgacg gggtttgtcc ccggcgaccg
      481 ggtggcggtg gatcccgtca caagctgcgg aacctgctat ccctgttcca taggccgccc
      541 caacgtgtgt gagcggctgg aggttttcgg cgttcacagg gacggcggtt tcgcggagta
      601 cattgtcctc ccagaaaaga atctccacag gattccctcc gactggtcct tcgaaaaggg
      661 agccctcgtc gagcccttca ccatcgcggc caacgtcctt tcacggacgg agtgctcggg
      721 aagcgaccgg ctgcttatcc tcggggccgg acccatcggc caggtgatcc tgcaggctgc
      781 ccgaaggctc ggaacggcct gcgccgtggc cgacatcgtt ggtgtgcggc ttgagaaagc
      841 ccggtccctc ggagcggagc tcacggtcaa cacccgggaa acccccctgg aagagacaat
      901 ggctgaatgg accggagggg ccggggtgcc cctcatcatc gacgccgttg gcagccccga
      961 cctttttccg tccctgctcc gcatggcctc ccctgcgggc aggataggcc tgctcgggtt
     1021 ctcgaaagag ccttcctcct tcgtccagct cgaggcggtg aaaaaagagc tctccatctt
     1081 cg
//
LOCUS       DAOTHD010000914         1079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_164743_length_1079_cov_3.230469, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000914 DAOTHD010000000
VERSION     DAOTHD010000914.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164743_length_1079_cov_3.230469"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="P5219_12420"
     CDS             <1..248
                     /locus_tag="P5219_12420"
                     /inference="COORDINATES: protein motif:HMM:NF024639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRX27619.1"
                     /translation="GDSPSDLRFASNCGCPFVLVRTGWGKKTEREISPFTGIRENEKS
                     GTMFFIADDLRAAAGIIEQAETGFSTERGGFSFIDPS"
     gene            235..>1079
                     /locus_tag="P5219_12425"
     CDS             235..>1079
                     /locus_tag="P5219_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HRX27620.1"
                     /translation="MTLRKGLVLFLLLSFGVSAVVLLSSVDSDTWSTILRADKRLLLL
                     ALVFVLSAWACDASRFCALARSAGEKIDFKLGMVLTWLHYFGCAVTPMQSGGGPFQVY
                     VLYKRKVPLGKGIAITLTRTLLTILILGIVVPVAVFIEPELLRGRLFLKGVFSYVLAF
                     IIVSWVLVVLSVARPAVIKRWGRVLTLWLKRFGVVKPERVLKIVKRINLEVDNYNLNF
                     QMFFSCGLKYFLLAIVLSVFHLLFIFSVLPCLIASVGLPFHYLQAILAQAVFMFLLYF
                     VPTPGA"
BASE COUNT          180 a          337 c          274 g          288 t
ORIGIN      
        1 tcggggactc cccatccgac ctccggttcg cttccaactg cggatgtcct ttcgtcctcg
       61 tccggacggg ctggggaaaa aagacggaaa gagagatttc ccccttcacc ggaatcaggg
      121 aaaacgaaaa atctggtaca atgtttttca tcgccgacga tctccgcgct gccgccggta
      181 tcattgaaca agcggagacc ggtttttcca ccgaaagagg aggcttttcc ttcattgacc
      241 cttcgtaaag gcctggtgct tttcctgctg ctctccttcg gagtcagcgc cgtggtactt
      301 ctgtccagcg tggactccga tacctggtcc actattttgc gtgccgacaa gcggctcctg
      361 ttgcttgccc tcgtctttgt cctctcggcg tgggcctgtg atgcctcccg tttctgcgcc
      421 ctcgcccggt cagcggggga aaagatcgac ttcaaactcg gaatggtgct tacatggctc
      481 cattacttcg gctgtgccgt cactcccatg cagagcggag gaggaccttt ccaggtctac
      541 gttctctaca agcggaaggt tcccctcggg aaaggcatcg ccatcaccct cacccggacc
      601 ctgctcacca tccttatcct gggaatcgtg gttcccgtgg cggttttcat cgaaccggaa
      661 cttctcaggg ggcgactttt cctcaaggga gtcttttctt acgtcctcgc cttcatcatt
      721 gtctcctggg tcctggtggt gctcagcgtg gcgagaccgg ctgtcatcaa acggtggggc
      781 agggttctga cgctgtggct caagcgcttc ggcgtggtca agcctgagcg ggtgctcaaa
      841 attgtcaagc ggatcaacct cgaggtggac aactataatc tcaacttcca gatgttcttc
      901 tcctgcggtc tgaaatattt tctcctcgcc atcgttcttt cagtattcca cctgcttttc
      961 atcttctccg tgcttccctg ccttatcgcg tcggtaggcc ttcccttcca ctatctccag
     1021 gcgattctcg cccaggccgt cttcatgttc ctgctgtact tcgtccccac cccgggggc
//
LOCUS       DAOTHD010000915         1078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_164937_length_1078_cov_4.232649, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000915 DAOTHD010000000
VERSION     DAOTHD010000915.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164937_length_1078_cov_4.232649"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="P5219_12430"
     CDS             <1..960
                     /locus_tag="P5219_12430"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX27621.1"
                     /translation="PSLPQAETPGEEEPAADEVLELARKGDPEAQTAVGAMYYDGDGV
                     PKDTGKAVEWLEKGAAGGDAGAKALLGLIRIFEDGFTDPAAGIRLLREASSVPDADPE
                     IQGLLARLLYDGEMWGIERNLEECAEWAEKAAEGGDPDGMGLLALLCYFGEGADEDDE
                     RAFDLAEQSAAGNSSLGKAALAWIYYEGVAADEDLEKARLLALDAAEEEESLAQGLLA
                     WMYLKGHGVKEDTGAAEGWARRAAEQGNEFGWFVLGSLYFSGDGVERDLPAAWAYLDM
                     ADERAVVEAEELKEEAWKHMSKKERQRAEKLRSRWTEERDLPR"
BASE COUNT          231 a          310 c          374 g          163 t
ORIGIN      
        1 ccttccctcc cccaggcgga aacccccgga gaagaagagc ccgcagcgga cgaagtcctg
       61 gagcttgccc gtaaaggaga cccggaagcc cagactgccg tgggagccat gtattatgac
      121 ggtgacggcg ttccgaagga cacgggaaaa gccgtggaat ggctggaaaa aggggccgcc
      181 gggggagacg ccggggcaaa ggcccttctc ggcctgatca ggatcttcga agacggcttc
      241 acggatcccg cggcgggaat ccgcctcctg agggaggcat cttccgtacc ggacgccgat
      301 ccggagatac aggggctcct cgcccggctc ctctatgacg gggagatgtg ggggatagaa
      361 cgaaacctgg aggaatgcgc ggaatgggca gaaaaggcgg ctgaaggggg agatccggac
      421 ggcatggggc ttctggccct tctctgctat ttcggcgagg gagcggacga ggacgacgaa
      481 agggccttcg accttgccga acagtctgcc gccgggaaca gttccctcgg aaaggccgcc
      541 ctcgcgtgga tatattacga aggcgtcgcc gccgatgagg acctcgaaaa ggcccgtctc
      601 ctcgccctcg atgccgccga agaggaagaa tccctcgccc aggggctcct ggcatggatg
      661 tacctgaagg gacacggagt caaagaagac accggggccg cggaggggtg ggcccgccgg
      721 gcggcggaac aggggaacga attcgggtgg tttgtcctcg gatcgctgta tttctccgga
      781 gacggagtgg agcgggacct gcccgccgcg tgggcttatc tggacatggc cgatgaacgg
      841 gccgtcgtcg aggctgaaga actgaaagaa gaagcctgga aacacatgtc gaaaaaggaa
      901 aggcagcggg ccgaaaagct caggagccgc tggacggaag aaagggacct tccccgctga
      961 tcagcgggcg gaggacccct tcctccccat gtcgcacagt actttcgcga gaccgagctt
     1021 gtgttcccgg ataagccgct cggctctttc gccgtccccc ttctccagtg catcgagg
//
LOCUS       DAOTHD010000916         1078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_164971_length_1078_cov_3.014663, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000916 DAOTHD010000000
VERSION     DAOTHD010000916.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164971_length_1078_cov_3.014663"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..>1078
                     /locus_tag="P5219_12435"
     CDS             23..>1078
                     /locus_tag="P5219_12435"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRX27622.1"
                     /translation="MPPFRYCTEAVLHGEGLSRPGLIGGLSRYGDSIVVGGGGGRFRV
                     HVHTDRPAEVMDRLSGMGRVSFQKVDDMKRQVEARNSPLSTVGLLTDSCCDLPQELLD
                     RYQVHTVPLTVSSGGWEYLDKLTLKPGEIYTALRETGRTFTTSQPAFGEFVKRYSFLK
                     SCYDSVISLHLSSALSGTWSVSRKAADHAGGEGIHVLDSKNLSGALGLLVLKAAEAAA
                     AGKSCPEILEILRSAIPRTRIFAGLNSLRYLVRGGRAGAMAGFVGTMLNLFPMITVDP
                     EGKACGFGRPGSERANRRKMLDEFKRLTGSHGLWKYAVLHAEAPGAAEELAAALEEAC
                     GRAPEYTMEISPVIGINT"
BASE COUNT          210 a          325 c          354 g          189 t
ORIGIN      
        1 cgagcattcc cacggtgatg aaatgccccc gttccgctac tgtaccgagg ctgttctcca
       61 cggggagggg ctttcgcgcc ccgggctgat cggcggcctg tcccggtacg gggattccat
      121 cgtggtcggg ggaggcgggg gaagatttcg ggttcatgtc cataccgacc gtcccgccga
      181 ggtgatggac cgtctctcgg gcatggggag ggtaagcttc cagaaggtgg atgacatgaa
      241 gaggcaggtg gaggcgagga attctcccct ttccaccgtc ggcctgctga ccgactcctg
      301 ctgtgacctg ccccaggagc tgctggacag gtaccaggtg cacacagtac ccctcaccgt
      361 gtcatccggg ggatgggaat atcttgacaa gctgaccctg aagccgggag aaatttatac
      421 cgccctcagg gagaccggca gaaccttcac cacgtcccag ccggccttcg gcgaattcgt
      481 gaagcggtat tcctttctga aatcctgtta cgacagcgtc atttccctcc atctctcatc
      541 ggccctgagc ggtacatgga gcgtgagccg gaaggcggcc gaccatgcag gcggcgaggg
      601 aatccatgtt ctcgacagca aaaatctgtc cggcgccctc ggcctcctgg tgctgaaggc
      661 cgccgaagcg gccgcggcag gaaaaagctg cccggagatc ctggaaatcc tccggagcgc
      721 catccccagg acgaggatct tcgcagggct gaactccctc cgctatctcg taaggggagg
      781 ccgggcaggg gccatggcag gtttcgtggg gaccatgctg aacctgttcc ccatgatcac
      841 cgtggacccg gaagggaagg cctgcggctt cggcaggccc ggcagcgagc gggccaaccg
      901 gaggaagatg ctggacgagt tcaaaaggct gaccggcagc catggtttgt ggaaatacgc
      961 ggttctccac gccgaagccc ccggcgcggc ggaagagctg gcggccgccc tggaagaagc
     1021 ctgcggcagg gctcctgaat acaccatgga gatctcgccg gtgatcggaa tcaatacg
//
LOCUS       DAOTHD010000917         1078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_164978_length_1078_cov_2.869013, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000917 DAOTHD010000000
VERSION     DAOTHD010000917.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164978_length_1078_cov_2.869013"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..121)
                     /locus_tag="P5219_12440"
     tRNA            complement(24..121)
                     /locus_tag="P5219_12440"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(85..87),aa:Sec,seq:tca)
     gene            complement(617..>1078)
                     /locus_tag="P5219_12445"
     CDS             complement(617..>1078)
                     /locus_tag="P5219_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27623.1"
                     /translation="GVIYDRLLLVLSDVLFTRSGDGIRVLSYRDSRLSGTILKKDFLA
                     RVRQKMPHYALSELELKDGKVMVFGAYRRKGTFSMNALIRLTGQYVIDGAGKAMIRFD
                     DSTNDNPFISAADVGRAVAKAAPVLSFTEFFASPKVEEVRVGHDMVWFSAK"
BASE COUNT          264 a          295 c          285 g          234 t
ORIGIN      
        1 ccggctgttt atggcgatca ggttggcgga ggcgtgtggg aatcgaaccc accggagaca
       61 gcctatactg ccccccactg gatttgaagt ccagggcgcc caccgggaca cattcgcctc
      121 cccgccgaga ctatgataag ccatccaaca ataggcgtca acagcattca gaaagttttc
      181 ccctttgtct gacaaatagg cacaaaaaac aagaaggctg cgtagtccag tgcggctgga
      241 aaaacgctcc gtcggatcaa atgtcagcca tccacgaaaa aatccacaga atcacgaatc
      301 aagagagaac ggatgagatt ggaagagttt ctggcctgtt ttgccctttc ccctgaaacc
      361 agacgaccgc ccttcaccac aggcggtttt gccttcttct cccctgaggc gtaggatact
      421 ttggtgaaag ttcgcaaggg aaccaatatt ctgatgaatg gggatgaagc aggtggccca
      481 gcaggctcag aaaatgaaca tgacggcagt gattgttgtt ctaatagtag ccgcaatcgg
      541 catctacatg tttgtctcca ccatgaagta agggaatccg gctgaaaacg ggaacccctt
      601 tccggggttc ccgtttttat tttgcggaaa accagaccat gtcgtgtccg acccggacct
      661 cctccacttt cggagaggca aagaattcag tgaaggaaag caccggcgca gctttcgcca
      721 cggcccttcc cacgtcggcc gctgagatga aggggttgtc gttggtggaa tcgtcgaacc
      781 gtatcatggc ctttcccgct ccgtcaataa catactgccc cgtgaggcgg ataagggcat
      841 tcatgctgaa ggtccctttt ctccggtagg ctccgaatac catgactttc ccgtccttca
      901 gttccagttc ggaaagggcg tagtggggca ttttttgccg cacccgggcg agaaaatcct
      961 ttttcaggat agtccccgag aggcggctgt ccctgtacga aaggacccgt attccgtctc
     1021 ccgaacgggt gaaaaggaca tccgagagta ccagcagcag gcggtcgtaa atgacccc
//
LOCUS       DAOTHD010000918         1078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_164984_length_1078_cov_2.716520, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000918 DAOTHD010000000
VERSION     DAOTHD010000918.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164984_length_1078_cov_2.716520"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..584
                     /locus_tag="P5219_12450"
     CDS             189..584
                     /locus_tag="P5219_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRX27624.1"
                     /translation="MPESRVQKLQTFFMGEENVRLAFLIGSFAAGTARPDSDVDVAVL
                     FGRVPDYMDVLGLRDRLSVLMEREADLVVLNDAGPVIRMQVLKTGIRLRAEQGAYEKF
                     FVRTVNEYDDLKQIRAPIEEAVLRKKIYA"
     gene            577..999
                     /locus_tag="P5219_12455"
     CDS             577..999
                     /locus_tag="P5219_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRX27625.1"
                     /translation="MHDKDIILAKVASIQRCLCRIKEVTSLDPGSLENIDIQDIFLLN
                     LQRGIQAALDLAAHVVASEGLGLPERARDTFSLLKTAGILDGETASRMEKMTGFRNIA
                     VHGYQSLNPDILKSILAYRLKDLEDFYSAVLRHFPCKR"
BASE COUNT          226 a          311 c          316 g          225 t
ORIGIN      
        1 cgggaaggaa cggtgacctg gttctgacca cccggtgccg gagggtcccg ggcacggggt
       61 tttgattcgt gaagtcctgc accgctgttc cggaggcagt tctgacgtat tcgttccgtt
      121 ttttgtgctc cgccgctgat gtggtatcct tacgaaagag gcggctacgc ccgggagggg
      181 gaaagggaat gccggaaagc agagtgcaga agctccagac gttcttcatg ggagaagaga
      241 acgtccggct cgcgttcctt ataggctcct tcgctgcggg aacggcccgt cccgacagcg
      301 acgtggatgt tgccgtcctc ttcggccgcg ttccggatta catggatgtt ctcggcctcc
      361 gggacaggct gtccgtcctc atggagcggg aggccgactt ggtagtgctg aacgatgccg
      421 gccccgtcat ccgcatgcag gtactgaaga ccggcatccg gctccgggcg gaacaaggag
      481 cctacgaaaa atttttcgtc cgcacagtca acgagtatga cgacctgaag cagatccgcg
      541 cccctatcga agaggccgtg ctgaggaaga aaatctatgc atgacaaaga cataatcctc
      601 gcgaaagtcg cctccatcca gagatgcctg tgcaggataa aagaagtgac gtccctcgat
      661 ccggggagcc tcgaaaatat cgacatccag gacatctttc tcctcaacct gcagcgtgga
      721 atccaggccg cccttgacct tgcggctcac gtggtcgcat cggaggggct cggccttccg
      781 gaacgggcga gggacacttt ctccctgctg aagacggccg gaattctcga cggggagact
      841 gcctccagga tggagaagat gaccggtttc cgaaacatcg ccgtccatgg ctaccagtcc
      901 ctgaatccgg acatcctcaa atccatcctg gcgtaccggc tgaaagatct cgaagatttc
      961 tattccgccg tgctccgcca tttcccctgc aaaagataaa gggcgcccgg aaagttccgg
     1021 atgccctttt ttccgttatt tttcagggct gcatttcctg ccccatgaaa gcgatgga
//
LOCUS       DAOTHD010000919         1077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_165190_length_1077_cov_3.337573, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000919 DAOTHD010000000
VERSION     DAOTHD010000919.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165190_length_1077_cov_3.337573"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="P5219_12460"
     CDS             complement(<1..571)
                     /locus_tag="P5219_12460"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27626.1"
                     /translation="MTEKLALLFPGQNARVLEAMSSVPREIFVDPGWRRFAYSERSLP
                     IGHGQTISKPATVLRMLNSMEPVYGGRLLEVGSGSGYLAAVASRLFNKVFCVERVLGL
                     VEPSRANIGKTGARNVMVKYGDGSAGWPGHAPFDGILYSAGAPSFPESLKDQLAPGGL
                     AGVPVGPPDDQVFTVWRKEGDGLLEVFSFQ"
     gene            complement(662..738)
                     /locus_tag="P5219_12465"
     tRNA            complement(662..738)
                     /locus_tag="P5219_12465"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(702..704),aa:Pro,seq:ggg)
     gene            complement(769..>1077)
                     /locus_tag="P5219_12470"
     CDS             complement(769..>1077)
                     /locus_tag="P5219_12470"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRX27627.1"
                     /translation="HTLHYWETAFPALKPHKNHAGNRCYRPGDIELIKMIDRLVNREG
                     FSIEGARKQLDNMRKGNGQLSFDLVGDSAQERIIEQVCSELEAMIRDLSRDPRTKLDD
                     "
BASE COUNT          202 a          336 c          295 g          244 t
ORIGIN      
        1 actggaagga aaatacttcc agaagcccat ctccttcttt tctccaaacc gtgaacacct
       61 ggtcgtcagg gggacccacc ggtacccctg cgagcccgcc gggggccagc tggtccttca
      121 gagattcagg gaatgacggt gctccggcac tgtagaggat gccgtcgaag ggggcgtgcc
      181 cgggccatcc ggcgcttccg tcaccgtact tcaccatcac gttccttgct ccggtcttcc
      241 cgatgtttgc ccttgaaggc tcaaccagac ccagcaccct ttccacgcag aaaaccttgt
      301 tgaaaagtct ggaagccacc gcggccaggt acccgcttcc gctgcccact tcgaggagcc
      361 tgccgccgta aaccggctcc atggagttca gcatacgcag caccgttgcg ggtttgctga
      421 tggtctgacc gtgtccgatg ggaaggctcc gctcggagta cgcaaatctt ctccagccgg
      481 gatccacgaa gatctccctg ggaaccgacg acattgcctc caggaccctg gcgttctgcc
      541 ccgggaacaa aagcgcaagt ttttcggtca ttgcacgggc cctctgcgtc aactcatctc
      601 cgatttttcc ctgaatatgc ggaagaggaa ccgggaggtt cctcttcatg ctgcttctcc
      661 ttggtcgggg cgactggatt cgaaccagcg accacttgaa ccccattcaa gtgcgctacc
      721 ggactgcgcc acgccccgtc aggaagcaca attctatgca acgctgattc agtcgtcaag
      781 tttggtgcgg ggatcccggg acagatcgcg tatcatggct tcaagttcgg agcagacctg
      841 ttcgattatc cgttcctggg cgctgtctcc cacaaggtcg aaggaaagct gtccgttgcc
      901 cttcctcatg ttgtccagct gcttccttgc tccctcgatg gaaaagccct cccggttcac
      961 cagcctgtct atcatcttga tgagttctat gtcaccaggc ctgtagcacc tgttcccggc
     1021 gtggttcttg tggggcttga gggcggggaa ggcggtttcc cagtagtgga gagtatg
//
LOCUS       DAOTHD010000920         1076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_165465_length_1076_cov_2.780607, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000920 DAOTHD010000000
VERSION     DAOTHD010000920.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165465_length_1076_cov_2.780607"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="P5219_12475"
     CDS             complement(<1..822)
                     /locus_tag="P5219_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRX27628.1"
                     /translation="MHCDILIRNCSLLTPEYDVRESVSVAIDGSYIAFIGNAGEAEEQ
                     YTPRETLDGRGKLLMPGFVDAHTHTCQQLLRGRLADEYPMIWTRFLVPFESTLTPDDV
                     YWSAKLACLEMIRSGTTSFADSGGVHMHRAADAVIESGMRAAIARSTMDMGGEICSAM
                     KESAEDAIARTEELYRNYHGAGDGRVEIWFALRQVMTCSPELVRRTAEKAAEYSTGIH
                     AHLCEHRDEVRFCLERYRKRPAEFLDEMGALGPNLLTAHNVVLSEHDITLLAERQV"
     gene            complement(836..>1076)
                     /locus_tag="P5219_12480"
     CDS             complement(836..>1076)
                     /locus_tag="P5219_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27629.1"
                     /translation="LACPLSPFKEQVIAWANNFAFLAPATALGAFAGISLGKDMKNFG
                     RMGVKLIAITVLVITGTFIMSAFVADQVLKYTGSV"
BASE COUNT          181 a          348 c          343 g          204 t
ORIGIN      
        1 cacctgccgc tccgccagga gggtgatgtc atgttccgaa agcaccacgt tgtgggccgt
       61 caggaggttc ggtccgaggg ctcccatttc gtcgagaaac tcggctgggc gcttccggta
      121 acgctccagg cagaagcgga cttcgtcccg gtgctcgcag aggtgggcgt ggatccccgt
      181 gctgtattcc gcggcctttt ccgccgttct ccggaccagc tccggcgagc aggtcatcac
      241 ctgccggagg gcgaaccaga tctcgacccg gccgtccccc gcgccgtggt agttcctgta
      301 cagctcctcg gtccgggcga tggcgtcctc cgccgattcc ttcatggcgc tgcagatttc
      361 cccgcccatg tccatggtgg acctggcgat ggcggccctc atgcccgact cgatcaccgc
      421 gtccgccgcc cggtgcatgt ggacgccgcc cgaatcggcg aaggaggtgg tgccggacct
      481 gatcatctcc aggcaggcca gtttcgcgct ccagtacacg tcgtcaggag tcagggtgct
      541 ctcgaaggga acaagaaagc gggtccagat catggggtac tcgtcggcga gcctccccct
      601 cagcagctgc tggcaggtgt gggtatgggc gtctacgaac cccggcataa gcagcttgcc
      661 ccgtccgtcg agggtctccc ggggggtgta ttgctcctcc gcctcgccgg cattgccgat
      721 gaaggcgata tagctgccgt caatggccac cgacaccgat tccctgacgt catactcggg
      781 agtgaggagt gagcagttcc ggatcaggat gtcgcagtgc actttcccgg cctcctcaga
      841 cggatcccgt gtacttcagg acctggtccg ccacgaaggc cgacatgatg aaggtacccg
      901 ttatgaccag cacggtgatg gcgatcagct tgacgcccat cctgccgaag tttttcatgt
      961 cctttcccag ggagatgccc gcgaaggccc cgagggccgt ggccggagcg aggaacgcaa
     1021 agttattcgc ccaggcaatc acctgctcct tgaaggggga gagagggcag gccagc
//
LOCUS       DAOTHD010000921         1074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_165978_length_1074_cov_2.419038, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000921 DAOTHD010000000
VERSION     DAOTHD010000921.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165978_length_1074_cov_2.419038"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1074)
                     /locus_tag="P5219_12485"
     CDS             complement(<1..>1074)
                     /locus_tag="P5219_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27630.1"
                     /translation="AGMPVELLASAEGAKKGDLFFRFEPSTEVSFSPPESEEGRTKAV
                     FSEPGTVRIWAVALDGNGTLGESEQVEIEVVGPELTVTAMPPKPKVGEEVKAKAGAKP
                     GLPEGAFVVWTLEGKASRSGAKAADSTEYSFLPREAGKYVLKAEARSQGGETLAEKTL
                     AVEVTGYEVAVTVLGPAGPRPQEWVQGQGLRTVEKDTFLRDEHVRMKAEVKDADAPKD
                     LRWKWIPGPETSLASSGIGQEATLYGTAAGTASARVEVRDTEDLLLGAASVSFPVMEL
                     PPELKVPPLKGAVRADPEPGTVNGRITLSASAEGGKAPYTFRWGGPVTGSGDILTFIP
                     DRPGTYRFTLLVLDSAGRQVTVNR"
BASE COUNT          157 a          403 c          317 g          197 t
ORIGIN      
        1 cggttcaccg tgacctgccg ccccgccgaa tccaggacca gcagggtgaa ccggtacgtc
       61 cccggccggt cggggatgaa ggtgagaata tcccccgacc cggtaaccgg acctccccac
      121 cggaaggtat agggggcctt gcccccttct gcggaagcgg agagggtgat tctcccgttc
      181 accgtcccgg gctcggggtc cgccctgacg gcccccttca agggaggaac cttcagctcg
      241 gggggcagct ccatcacggg gaaagagacg gaggccgcgc ccagaagaag gtcctccgtg
      301 tcccgcacct ccacccgggc cgatgccgtt ccggcggcgg tgccgtacag agtcgcctcc
      361 tgcccgatgc ccgaagacgc cagggacgtc tccggccccg gaatccactt ccaccggaga
      421 tccttcggcg catcggcgtc cttcacttcc gccttcatcc ggacgtgctc gtcccggaga
      481 aaggtgtcct tttccacggt gcggaggccc tgcccctgca cccattcctg cggtcggggt
      541 cctgccgggc cgagaaccgt cacggccacc tcgtaccccg tcacctccac ggccagcgtt
      601 ttctccgcca gggtctcccc tccttgactt ctggcctccg ctttcaggac gtatttcccc
      661 gcctcccggg ggaggaagga atattccgtg gaatccgcgg ctttcgcccc gctgcgggac
      721 gcctttccct ccagggtcca cacgacgaag gccccttccg gcagtccggg cttcgccccg
      781 gccttcgctt tcacttcctc ccctaccttc ggcttcgggg gcatggccgt gacggtcagt
      841 tcaggcccga ccacttctat ttcgacctgt tccgattcgc cgagggtgcc gttcccgtcc
      901 agggcaacgg cccagatccg caccgttccg ggttcggaaa agacggcttt cgtccgcccc
      961 tcctccgatt ccggagggga gaaggagacc tccgtggagg gctcgaaccg gaagaacagg
     1021 tctcctttct tcgctccttc cgccgacgcc aggagttcca ccggcattcc ggcg
//
LOCUS       DAOTHD010000922         1073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_166145_length_1073_cov_2.916503, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000922 DAOTHD010000000
VERSION     DAOTHD010000922.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166145_length_1073_cov_2.916503"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1073)
                     /locus_tag="P5219_12490"
     CDS             complement(<1..>1073)
                     /locus_tag="P5219_12490"
                     /inference="COORDINATES: protein motif:HMM:NF012431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRX27631.1"
                     /translation="HSFLVRSVEELPAALKGAFEIAVSGRPGPVLVDLPVDIQKAEGE
                     FIYPEHTLYGNGRLKMQTDLSRADEAAALLRGAERPVILAGGGILSSGASDFLLRLAE
                     KCGIPVATTLMGKGAFPEDHPLSLGMAGMHGTPWANLALSEADVLFAVGTRFSDRTTG
                     RLDCFAKDAAVIHGDIDEAEFDKIVPCRVHLLGDAKAILGTVAEKLGKCVRDEWTATV
                     ERWKEEYPLSDPGSADFVPSSVLEAVRAAAKDKWPVVADVGQNQMWTALFYRTNEPGT
                     FFTSGGLGTMGYALPAAMGASLARNRTPVLCFAGDGGFLMNCQELETCARYGIPVKIF
                     VLNNSALGMVRQWQELFWNERYS"
BASE COUNT          162 a          344 c          387 g          180 t
ORIGIN      
        1 ggagtaccgt tcgttccaga agagttcctg ccactggcgg accatgccga gggcgctgtt
       61 gttcaggacg aagattttca ccgggatgcc gtaccgggcg caggtttcga gctcctggca
      121 gttcatcagg aagccgccgt ctcctgcgaa gcagagcacg ggggtgcggt ttctggccag
      181 ggaggcgccc atggccgccg ggagggcgta ccccatggtg ccgaggcccc cggaggtgaa
      241 gaaggttccg ggctcgtttg tcctgtagaa cagggcggtc cacatctggt tctggcccac
      301 gtcggccacc acgggccact tgtccttcgc cgctgctcgc acggcctcca ggaccgagga
      361 cggaacgaag tccgctgagc ccgggtcgga gagggggtac tcctccttcc accgttccac
      421 cgtggccgtc cactcgtccc gtacgcattt ccccagcttt tccgcgaccg tgccgaggat
      481 ggcctttgcg tcgccgagga ggtgcacccg gcacgggacg atcttgtcga actccgcctc
      541 gtcgatgtcg ccgtggatga cggcggcgtc tttggcgaag cagtcgagcc tgccggtggt
      601 gcggtcgctg aaccgggtgc ccacggcgaa gaggacgtcc gcctccgaaa gggcgaggtt
      661 ggcccagggg gtgccgtgca tgcccgccat gcccagggag aggggatggt cctcggggaa
      721 ggcccccttc cccatgaggg tggtggccac ggggatgccg catttctccg ccaggcggag
      781 gaggaagtcg gaagcaccgg aggagaggat gccgccccct gcgaggatca ccggccgttc
      841 tgcgccccgg aggagggcgg cggcctcgtc cgcccgggac aggtcggtct gcatcttcag
      901 gcggccgttg ccgtaaaggg tatgttccgg gtagatgaac tcgccctcgg ccttctggat
      961 gtccacgggc aggtccacga gcacggggcc ggggcggccc gagacggcga tctcgaaggc
     1021 ccccttcagc gccgccggga gctcctccac ggatcggacg aggaagctgt gct
//
LOCUS       DAOTHD010000923         1073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_166209_length_1073_cov_2.283890, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000923 DAOTHD010000000
VERSION     DAOTHD010000923.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166209_length_1073_cov_2.283890"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1073)
                     /locus_tag="P5219_12495"
     CDS             complement(<1..>1073)
                     /locus_tag="P5219_12495"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRX27632.1"
                     /translation="VGDLKQSIYRFRHADPTLFGSLILRQREGDRYIPLQASFRTRSS
                     LLERINGIFGHVWRNGLSSSLPQEYEPLVFPGGPDQAKLREDTALPPLDTVIRYARPQ
                     ENGEGRKKTEPTGKVRVRVARGLAAKLAAFRGMDVWDKKEGRMRPARWKDMAVLVPAR
                     TSFPALEMVFRSEAAIPAAFEKGKEYFSRGEIEDLAAAIRTIAFPEDRGALAAFLCTH
                     FSGLSLDEAYALLPAGEDFPVRHPGAAARLEALRAEARYSGLFAALCSLLRDQSYLRA
                     YPAWNRRNVLANLRQALDMVREYESVFGPDPAGCTGYFASLRSGQGTVTETSPLGDEE
                     DVVRVMTVHSAKGLEFPIVAVMD"
BASE COUNT          173 a          362 c          364 g          174 t
ORIGIN      
        1 gtccatcacg gctacgatgg ggaactccag tcccttggcg ctgtggacgg tcatcacccg
       61 taccacgtcc tcctcgtccc ccaggggcga cgtctccgtc acggtcccct gcccggaccg
      121 cagggaggcg aaataaccgg tgcaccctgc cgggtccggt ccgaagacgg actcgtactc
      181 ccgcaccatg tccagggcct gccggagatt ggcaaggacg ttccggcggt tccaggcagg
      241 ataggcgcga aggtacgact ggtcccggag aagggagcag agggcggcga acagtccgct
      301 gtatctcgcc tccgcccgga gcgcttccag ccgggccgcc gccccggggt gccgcaccgg
      361 gaagtcctcc ccggcgggaa gaagggcgta ggcctcatcc agggagagcc cggagaagtg
      421 ggtgcagagg aaggccgcca gggccccccg gtcctccgga aaggcgatgg tccggatggc
      481 tgcggcaaga tcttctatct ccccccggct gaagtattcc ttccccttct cgaaggccgc
      541 cgggatggcc gcctcggacc ggaacaccat ctccagggcg ggaaaggacg tccgggccgg
      601 cacgaggaca gccatgtcct tccaccgggc gggccgcatg cgtccctctt tcttgtccca
      661 tacgtccatc ccgcggaaag ccgccagctt cgccgcgagg ccccgggcta ccctgacccg
      721 caccttgccc gtgggctccg tcttctttct tccctcgccg ttttcctggg gccgggcgta
      781 ccggatgacc gtgtccaggg ggggaagggc ggtgtcctcc cgcagctttg cctgatccgg
      841 accgccggga aagaccagag gctcgtattc ttggggcagg gaggaggaaa ggccgttccg
      901 ccagacatgg ccgaagatcc cgttgatcct ttccagcagg gaggaccggg tgcggaagct
      961 cgcctggagg gggatgtacc tgtctccttc cctctggcgc agaatcaggc tgccgaagag
     1021 ggtggggtcg gcgtggcgga agcggtagat ggactgcttc aggtccccca cca
//
LOCUS       DAOTHD010000924         1071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_166660_length_1071_cov_2.674213, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000924 DAOTHD010000000
VERSION     DAOTHD010000924.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166660_length_1071_cov_2.674213"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="P5219_12500"
     CDS             complement(<1..564)
                     /locus_tag="P5219_12500"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRX27633.1"
                     /translation="MDLSAFFSPRTMAVVGVSSGNFQHPANVVFAKNRHRYPVEVFPV
                     SPRGGTLQGREVFERVGDIPERIDLAVIAVKADAVPDVLVDCAAAGVGGAAVISGGFT
                     EVGRQDLQDRLAAIAREASMPLIGPNCLGIYVPSRVDTFFLPSERMVKPRLGNVTFVS
                     QSGGILVDVMIRFAQEGVGLARAVSIGN"
BASE COUNT          223 a          323 c          326 g          199 t
ORIGIN      
        1 attgccgatg ctgacggccc tggcgagccc gaccccttcc tgggcgaacc gtatcatcac
       61 gtccacgagg atgccgccgc tctggctcac gaaggtgacg ttgcccaggc ggggcttcac
      121 catgcgctcg ctgggcagga agaaggtgtc cacgcgtgac ggcacgtaga tgcccaggca
      181 gttggggccg atgagcggca tcgaggcctc cctggcgatg gcggcgagcc tgtcctgcag
      241 gtcctgcctg ccgacctcgg tgaacccgcc ggagatgacc gccgctccgc cgacacccgc
      301 ggcagcgcag tcgacgagca cgtcgggcac ggcatcggcc ttcaccgcga tgacggcgag
      361 gtctatccgc tcggggatgt ccccgacacg ctcgaagacc tcccggccct ggagggtgcc
      421 cccgcgcggg ctcaccggga agacctccac cgggtagcgg tgcctgttct tggcaaagac
      481 cacgttggcc gggtgctgga agttgcccga cgacaccccc acgacggcca tggtcctggg
      541 tgagaagaag gcgctgagat ccatgggaca tcctcctcgt gtggcgaaag ctgatgactg
      601 gaataataca tgcccgaggc attcatggcc agtggtaaag aagattcccg ttctcgtccc
      661 tgctggaagg ggcaggtcaa cggagggcca tgaggggcta gaccgtgtac cccacagaaa
      721 atggggtcgg agtcgatttt ccaatggcag ccactggaaa atggagtcag ggttgttttt
      781 catctacgac atgatctggt acaataatct acaggtatat cgactccgag cccattatta
      841 tgatcacatt ttatccctta cgagaaaatc gactctgacc ccattttatc cgctgacccc
      901 attttacgga ccccatttta cgagaaaatc gactctgacc ccattttagt acgaggcatt
      961 catggccagt ggtaaagaag attcccgttc ccctccctgc gggaaggggc aggacaccaa
     1021 aggaccgtga ggggctagaa cgtgtagccc accgagatgt ggaagcggcc g
//
LOCUS       DAOTHD010000925         1069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_167138_length_1069_cov_2.631164, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000925 DAOTHD010000000
VERSION     DAOTHD010000925.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167138_length_1069_cov_2.631164"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="P5219_12505"
     CDS             <1..965
                     /locus_tag="P5219_12505"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HRX27634.1"
                     /translation="GPWKTPEHDGGEYETVGSFTAYVLNEDVDCAVHCGYLCNELGID
                     TISTGAVIGFLFECAEKGLVSLEAGGGLDLSWGNGAVLPRLVEMIALREGIGDILADG
                     VRRAAETIGGGAERFAVHVKGLEGPCHDPRAGKTLAVSYGTGNRGMCHIHPLETVAYD
                     CSHTDFGLIPFGLPDPNQFGRWDEEGKGEATRILQDGGALPDILGTCKFYMYVGITPK
                     EYAAMLSALTGWKVTGEELLGAAERVTNLQRLFNCREGFGREQDRLPDRARSLPAFGK
                     YAGSPESAVRNYDLMLDEYYDARGWDRKTGAPLEETLLRLGIEG"
BASE COUNT          229 a          295 c          374 g          171 t
ORIGIN      
        1 acggtccctg gaagactccc gaacacgacg ggggagagta cgagaccgtg ggatccttca
       61 ccgcctatgt tctcaacgag gacgtggact gtgccgtgca ctgcgggtac ctctgcaacg
      121 agctcggcat cgatacaatc tccacgggag cggtcatcgg cttcctgttc gagtgcgcgg
      181 agaagggcct ggtctcgctg gaggcagggg ggggcctgga tctctcctgg gggaacggcg
      241 ccgttcttcc ccggctcgtg gagatgattg ctctcaggga aggcatcggt gatattctcg
      301 ccgacggcgt caggcgggcg gcggaaacaa tcggcggagg ggcggagcgg ttcgccgtcc
      361 acgtgaaggg gctggagggc ccctgccacg acccccgggc cggcaagacc cttgccgtca
      421 gctacggcac gggcaaccgg ggcatgtgcc acatccatcc ccttgaaacg gtggcgtacg
      481 actgctccca caccgatttc gggctgatcc ccttcggcct gcccgacccg aatcagttcg
      541 gccggtggga cgaggaagga aagggcgagg cgaccaggat cctgcaggac ggaggggccc
      601 tccccgatat cctcggaacc tgcaagtttt acatgtacgt gggcataacc ccgaaggaat
      661 atgcggccat gctctcggcg ctgacagggt ggaaggtgac gggggaggag cttctggggg
      721 cggcggagcg ggtgacgaac ctgcagcgac ttttcaactg ccgggagggc ttcggaagag
      781 agcaggaccg tcttccggac agggcgcggt ccctgcctgc cttcgggaaa tatgccggca
      841 gtccggaaag cgccgtccgg aactacgacc tcatgctgga cgaatattac gacgccaggg
      901 gatgggacag gaaaaccggc gcccccctgg aggaaaccct ccttcggctg gggattgagg
      961 gatagaagac aggatgcaca aaacatttgc acggaaaagg cgggagacaa acgggggaaa
     1021 aaaaggtaaa atatgagcgt attatgtaaa gaacaggatg atgacgagg
//
LOCUS       DAOTHD010000926         1066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_167848_length_1066_cov_2.267062, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000926 DAOTHD010000000
VERSION     DAOTHD010000926.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167848_length_1066_cov_2.267062"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="P5219_12510"
     CDS             <1..313
                     /locus_tag="P5219_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRX27635.1"
                     /translation="GYILRRHLLPETLPVLLATMTLGVPGAILSEAGLSFLGLSDPRV
                     ISWGRMLHEAQSFGAFTAGAWWMLLPPGLGIALLCLVFLDLGKYLEERVDPRLRGGNV
                     L"
     gene            310..>1066
                     /locus_tag="P5219_12515"
     CDS             310..>1066
                     /locus_tag="P5219_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX27636.1"
                     /translation="MIDIRNLSVTYGRMTSEGRGSQAAAVKNVSMTIRKGQFSALAGE
                     SGSGKSTVLMAIPGLLPPGTAVSGEILLDGKNLLALPENDLNRIRWRRIAIVPQGAMN
                     SFTPVLTVGRHVEEVLSVHLGLSRKEALERIPSLFDLAGLDGELARRYPHELSGGQKQ
                     RAAIALALACSPDYLLCDEPTTALDVITQQGIIATLKNLVEKKGLGLLLISHDLPLAA
                     SVADRLHILKDGELVEEGDPAEITARPRNPHTRA"
BASE COUNT          189 a          379 c          317 g          181 t
ORIGIN      
        1 tggctatatc ctccggcgcc acctcctgcc ggagaccctc cccgtgctgc tggccaccat
       61 gaccctgggc gttcccgggg ccattctctc cgaggcgggg ctctccttcc tcggcctctc
      121 ggaccccagg gtgatctcct ggggaaggat gctccacgaa gcccagtctt tcggcgcctt
      181 cacggcagga gcgtggtgga tgctcctgcc ccccggcctc ggcatcgccc tcctctgcct
      241 cgtctttctg gaccttggaa aataccttga agagcgggtc gacccccgcc tgagaggagg
      301 aaatgtcctg tgatcgacat acggaatctc tccgtcacct acggccggat gacctccgaa
      361 ggaaggggaa gccaggccgc cgctgtgaaa aacgtctcca tgaccatccg gaaaggacag
      421 ttctccgccc tcgccggcga atcggggagc ggcaagagca cggtgctcat ggccatcccg
      481 gggctgcttc ccccgggcac agccgtctcg ggggaaatcc ttctggacgg aaaaaacctc
      541 ctcgccctgc cggagaacga cctgaaccgc atccggtggc ggaggatcgc catcgtcccc
      601 cagggggcca tgaactcctt cacccctgtg ctcaccgtgg gccgccacgt ggaggaagtg
      661 ctctccgtcc acctggggct ctcccggaag gaagccctgg aacggatacc ctcactcttc
      721 gacctggccg ggctggacgg ggaactggcc cgacggtacc cccacgaact ttcgggaggg
      781 cagaaacagc gggcggccat tgctctcgcc ctggcctgct ctccggacta ccttctctgc
      841 gacgaaccca ccacggccct ggacgtgatc acccagcagg ggatcatcgc caccctgaag
      901 aatctcgtgg agaaaaaggg gctcggcctt ctcctcatct cccacgacct gcctcttgcg
      961 gcttccgtgg cggacaggct gcacatactg aaggacggag agctggtgga ggagggagat
     1021 cccgccgaga tcaccgccag gccccggaat ccccacacca gggcct
//
LOCUS       DAOTHD010000927         1065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_168004_length_1065_cov_3.404950, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000927 DAOTHD010000000
VERSION     DAOTHD010000927.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168004_length_1065_cov_3.404950"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..387)
                     /locus_tag="P5219_12520"
     CDS             complement(163..387)
                     /locus_tag="P5219_12520"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HRX27637.1"
                     /translation="MTPLPALTGKLLVTALSKAGFNVVRIRGSHYFLRHNDGRCTVVP
                     VHAGETIGHGLLAKILKDCDLSRETLEQLL"
     gene            complement(384..599)
                     /locus_tag="P5219_12525"
     CDS             complement(384..599)
                     /locus_tag="P5219_12525"
                     /inference="COORDINATES: protein motif:HMM:NF027249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRX27638.1"
                     /translation="MTRQFSVIIEQDRDGYYVASVPSLRGCHTQAKSLDELSVRIKEA
                     IELCLEVEGDDPEFLDFIGVQQVSVPV"
     gene            751..>1065
                     /locus_tag="P5219_12530"
     CDS             751..>1065
                     /locus_tag="P5219_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HRX27639.1"
                     /translation="MNVSEYLAVARGDRPADLVLRGARVANVFTLEYEEADVALYGDR
                     IAGVGKGYGGVREEDLSGMVIVPGFIDGHCHIESTMLTPAAFSELAAVRGTTSAAPDP
                     HEI"
BASE COUNT          238 a          293 c          325 g          209 t
ORIGIN      
        1 caggagggat catgccggac tgaggaaaat ggttacggtg agctcttcaa gaggcatggg
       61 ccgaaggagt atgggttccc gaaggaaccg ctgcccgggg atggggggcg gtgtttcgaa
      121 aaaaactgat tccgggaaaa tggcagaatc agcctggatg tcctaaagaa gctgttcgag
      181 agtctcccgg gaaaggtcgc agtctttgag gattttcgcc agcagaccgt gccctatggt
      241 ttcaccggca tgaacaggga caacggtgca gcggccgtca ttgtgccgca gaaaataatg
      301 gctgcccctg atacggacaa cattgaatcc tgcttttgac agggcggtca ccagtaattt
      361 tccggtgagg gcaggcagcg gcgtcatacc ggaacgctga cctgctgaac cccgatgaag
      421 tcaagaaatt ccggatcgtc gccctccact tcaaggcaca gctcgattgc ctcctttatg
      481 cggactgaaa gctcatcaag cgattttgcc tgggtatggc atccccgcag ggaaggtacc
      541 gaagcgacgt aataaccgtc ccggtcctgc tcaatgatca cactgaattg cctggtcatg
      601 gactgtcacc tccccgaatg tgcaagagaa ttctatcatg atcacgccgc gcatccaaga
      661 gagggaaggc ggaagcgatg tcttccggta ttgcgctccg caaccctttc cgctacactt
      721 caccatggaa aagaacgggg ggatgaacct gtgaatgttt cggaatatct cgctgttgcc
      781 aggggtgacc gccccgcgga cctggtgctc cggggagccc gggtggccaa cgtcttcacc
      841 ctggagtacg aagaagcgga cgtggccctt tacggcgacc gcatcgccgg ggtcgggaag
      901 ggctacgggg gcgtcaggga agaagacctc tcggggatgg tgatcgtccc cggtttcatt
      961 gacggccact gccacatcga gagcaccatg ctcaccccgg ccgctttttc ggaactcgcc
     1021 gcggtaaggg gcaccacttc ggcggcgcct gatccccacg agata
//
LOCUS       DAOTHD010000928         1064 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_168271_length_1064_cov_2.994054, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000928 DAOTHD010000000
VERSION     DAOTHD010000928.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168271_length_1064_cov_2.994054"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="P5219_12535"
     CDS             <1..412
                     /locus_tag="P5219_12535"
                     /inference="COORDINATES: protein motif:HMM:NF020135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HRX27640.1"
                     /translation="IRTAVSENILVDSWSKYALMCGNSVMFCYYDGPAGTVREDPQYE
                     SVLRAVTGELVAVAAAKGVALPADTAEKAAAFLSGMQPDAMSSLYRDLSSGKPADETE
                     LDHIIGRMVEMGRQTGVPTPYHTIAYERFASRQD"
     gene            609..854
                     /locus_tag="P5219_12540"
     CDS             609..854
                     /locus_tag="P5219_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HRX27641.1"
                     /translation="MKKPEIDRNKIYSSSEIIRILEADGWQLHGTEGSHCQFRHPVKK
                     GKVTVKHPDKNVPGPTAKSIWKQAGLLPQPSRKEEDS"
     gene            851..>1064
                     /locus_tag="P5219_12545"
     CDS             851..>1064
                     /locus_tag="P5219_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRX27642.1"
                     /translation="MNKPDRYRYPAVMGYDDETKIFYLLWPDLPGCTTTGKTEDEALK
                     NAAEAMSLHLWGMEDDGDEIPAPTPLQ"
BASE COUNT          285 a          264 c          301 g          214 t
ORIGIN      
        1 catcaggacg gcagtatcag agaatatcct tgtggacagc tggtccaagt atgccctgat
       61 gtgcggcaac agtgtgatgt tttgctatta cgacggcccc gcaggcacgg tgcgcgagga
      121 tccgcagtat gaaagcgtgc ttcgggccgt aacgggcgaa ctggtggcgg tggcggccgc
      181 aaagggagta gcgctgccgg cggatacggc ggaaaaagcg gcagcttttc tttccgggat
      241 gcagccggac gccatgtcct ccctctatcg tgatctcagc agcggcaagc cggctgacga
      301 aaccgaactc gaccatatca tcggaagaat ggttgagatg ggacggcaga ccggcgtgcc
      361 gaccccgtac cacacgattg cttacgaacg gttcgcctca cggcaggatt aacgcccgaa
      421 agccgagaac aaagagaagg gccctaagaa aacaattttc tcggactcct ttttttgggg
      481 aacagagcag cgcgaaatat tgatctgcag ggatttttcg atgaatttaa aaactctccg
      541 gatactcttc atttccgtga caaatattat taacacgtgt tataatcttg acgaaaggaa
      601 tgccccggat gaaaaagccg gaaatcgacc gcaacaagat ctactcctca tcggaaatca
      661 tcaggattct tgaggctgat ggatggcagc tccacggaac ggaagggagc cattgccaat
      721 tcaggcatcc tgtgaaaaag ggcaaggtta cggttaagca tccggataag aatgttccgg
      781 gtccgacggc aaaatcaatt tggaaacagg caggcctttt gcctcagcct tcccggaaag
      841 aggaggattc atgaacaagc ctgacaggta cagatatccg gcagtgatgg ggtatgacga
      901 tgaaacgaaa atattttatc ttctctggcc cgacctcccc gggtgcacca caacggggaa
      961 aacggaagac gaagctctga agaatgctgc cgaagccatg tcgctgcacc tttggggcat
     1021 ggaggatgac ggtgacgaaa ttccggcccc tacgccactt caga
//
LOCUS       DAOTHD010000929         1061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_169028_length_1061_cov_3.376740, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000929 DAOTHD010000000
VERSION     DAOTHD010000929.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169028_length_1061_cov_3.376740"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="P5219_12550"
     CDS             <1..609
                     /locus_tag="P5219_12550"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HRX27643.1"
                     /translation="KDDLSAGISGNGDGEKGEGRQSMSGKNDGTGAALFSQRPAQGTE
                     GVRTERFPVPGGFARELALAGRNGEALEDGMHNVVRFMRTEGHHRASMIVDPPALGRV
                     EIELASTSGGVEASIRVGNEQLRQLVQDQITVLRTHLQQQGVQVAELTVDIRDSGREG
                     HGEARDGAKDRNARVSGMAGTAEEDIPSFAVDLEKGLLHWVA"
     gene            621..1037
                     /gene="flgD"
                     /locus_tag="P5219_12555"
     CDS             621..1037
                     /gene="flgD"
                     /locus_tag="P5219_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HRX27644.1"
                     /translation="MTVSAVNSANETNYTAAANEALRETNSELGKDAFLKILIAQLSN
                     QDPLDPLKDKDFIAQMAQFSTLEQMTNMNKSIEQMNAITKGSAVNYIGRVVEYLDDEG
                     LSTYGTVAYVRFDTSGVILTTTEEIDIPLEKVVAVG"
BASE COUNT          274 a          276 c          346 g          165 t
ORIGIN      
        1 aaagatgacc tttctgccgg aatttcaggg aacggcgacg gagagaaggg agagggcaga
       61 cagtccatgt ccggaaagaa cgacggcacc ggggcagccc tgttttctca gcggcctgcc
      121 caggggacgg agggggtccg tacggaaaga ttccccgttc cgggagggtt cgcccgggag
      181 ctggccctgg ccggaagaaa cggtgaagcc cttgaggacg ggatgcacaa cgtggtcagg
      241 ttcatgagga cggaagggca ccaccgggcc tccatgatcg tcgatcctcc ggccctcgga
      301 agggtggaga tcgaacttgc ctcgacaagc ggcggagtgg aagcatccat acgggtaggc
      361 aatgaacagc tccggcagct tgtccaggac cagataacgg ttctgaggac ccatctccag
      421 cagcagggag tccaggtggc cgaactgacg gttgacatca gggacagcgg cagagagggg
      481 cacggagaag ccagggacgg tgcgaaagac aggaatgcca gagtgtccgg catggccggg
      541 acggcagaag aagacatccc atcctttgcg gtggatctgg aaaaagggct ccttcactgg
      601 gtggcctagg aggtggagac atgaccgttt cggcagtgaa cagcgcgaat gaaacgaact
      661 atacggcggc ggcaaatgag gctctcaggg aaacgaacag cgaactcgga aaggatgcct
      721 tcctgaaaat actcatcgcc cagctgagca accaggatcc tctcgatcct ctcaaggaca
      781 aggatttcat cgcccagatg gcccagttca gcaccctgga gcagatgacc aacatgaaca
      841 agagcatcga gcagatgaat gccattacca agggatccgc ggtgaactac atcggcaggg
      901 tcgtggaata cctcgatgac gagggactga gcacctacgg aacggtggcc tatgtccggt
      961 tcgacacatc gggcgtcatc ctcaccacca ccgaagagat cgatatacct ctcgaaaagg
     1021 ttgtggctgt ggggtaacgg acttcagaaa caggggcaaa c
//
LOCUS       DAOTHD010000930         1057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_170068_length_1057_cov_4.220559, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000930 DAOTHD010000000
VERSION     DAOTHD010000930.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170068_length_1057_cov_4.220559"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            337..>1057
                     /gene="nudC"
                     /locus_tag="P5219_12560"
     CDS             337..>1057
                     /gene="nudC"
                     /locus_tag="P5219_12560"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HRX27645.1"
                     /translation="MTFSRPGGEWLLFHKDLLLVKSKTPLSLPSSEDEPLFRDRLTVS
                     GILPPTRGSFPWGEYPGDDAPPPGYEGVGLRELWALGGEPLFHAAGTAFQMMDWKRNS
                     RFCPRCGAPMNLADSDRAYGCPECAYMAYPILSPAVIVAVTRDDRLLLARSPHFPKGR
                     YSVLAGFVEPGESLEETVEREIFEEVGIRVKNIAYFGSQPWPFPHSLMLGFTAEWAGG
                     ELAPDGKEIEDAGWFTPRTMPE"
BASE COUNT          203 a          352 c          325 g          177 t
ORIGIN      
        1 ccgggcatca cggccatcct cccgtccact acgctccgcc gaaaggcgga ccgggccacc
       61 atccccctca ctacggacct ccgccgaaag gtccggggaa ggacaagccg aaggcgccgc
      121 cccccaagca cgggcctccg ccgaaacacg gaaagaagaa atagcgcccg gctgtgaaga
      181 atccgaagag aagagccccc gcagatactc cgggggctct tctctttggg atataatgtt
      241 tctgtccgtt ccgcccgggc agtgcgggat tttcggaagg gaaatccgct gccgcgcccg
      301 gcagggacaa aaaatacaga cattggaggc ccttttatga cgttctcccg ccccggaggc
      361 gaatggctgc tctttcacaa ggatcttctg ctcgtcaaat cgaaaactcc tctttccctc
      421 ccgtcgtcgg aggatgaacc cctcttccgg gaccgcctga cggtctcagg catccttcct
      481 ccgaccaggg gaagcttccc ctggggggaa tatcccggcg acgatgcccc gcccccggga
      541 tatgaaggag tgggcctgcg ggaactgtgg gccctcgggg gagaacctct cttccatgcg
      601 gcgggcacgg ccttccagat gatggactgg aagcggaaca gcaggttctg cccgcggtgc
      661 ggcgccccca tgaacctcgc ggacagcgat cgtgcctatg gctgcccgga atgcgcctac
      721 atggcctatc ccatcctcag ccccgcggtc atcgtggcgg tgaccaggga cgacaggctc
      781 ctccttgccc ggagccccca ctttccgaag ggacggtaca gcgtcctcgc cggcttcgtg
      841 gagcccggag agagcctcga ggagacagtg gagcgggaaa ttttcgagga agtcggcatt
      901 cgggtaaaga acatcgccta tttcggaagc cagccctggc ccttccccca ttccctcatg
      961 ctggggttca cggccgaatg ggccggcggg gagctggctc ccgacgggaa ggagatcgag
     1021 gatgccgggt ggttcacgcc ccgcaccatg cccgaga
//
LOCUS       DAOTHD010000931         1057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_170172_length_1057_cov_2.195609, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000931 DAOTHD010000000
VERSION     DAOTHD010000931.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170172_length_1057_cov_2.195609"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="P5219_12565"
     CDS             complement(<1..355)
                     /locus_tag="P5219_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27646.1"
                     /translation="MSKSIVAKLGLLVAVSMILLAGSLAFMAQRGVDQMSSSVERVAE
                     EMLNEDIQNKNANDERSAQAYGEAMSGYLAVISATPLWDFNEESLKDYAKDMLNVPNV
                     AYAVIYAQDGSKMAGE"
     gene            800..>1057
                     /locus_tag="P5219_12570"
     CDS             800..>1057
                     /locus_tag="P5219_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRX27647.1"
                     /translation="MMKFLKAILVVVFCLVSAGAWALTADDVVYMTEEYPPFNMAGSD
                     GVATGASVEVMAGIFERMGASKTVKDIQVLPWARGYKEVQNT"
BASE COUNT          256 a          246 c          292 g          263 t
ORIGIN      
        1 tttccccggc cattttcgag ccgtcctggg cgtagatgac ggcataggcc acattcggca
       61 cgttgagcat gtccttcgcg tagtccttca gcgattcttc gttgaagtcc cacaagggag
      121 tggcggaaat gaccgccagg tatccggaca tggcttctcc gtaggcctgg gcgctccgtt
      181 catcgttggc gttcttgttc tggatgtcct cgttgagcat ctcttcagcg acgcgctcga
      241 ccgaggaaga catctggtcc actcctctct gggccataaa cgccagcgag cccgcaagga
      301 gaatcatgct gaccgctacc agcaatccga gttttgcaac gatgcttttc gacacgtgca
      361 ataccccctc attgatatga tgccgcggat gatgcccgtc gacactgaac aaagtcttca
      421 gaagagcgct attcgatgaa aaaattccca agaaagaaag cggggttgcc ggaccggatt
      481 ttattttgga aaatctgatg aaattatgaa ctattcctta atgtaggata gctcgaaaac
      541 ggttttattg caagacactc tgcggaaaag tatgcatgaa tactgccagg ttcattgaaa
      601 tgcggcggtg aacgtatgcg agaaaatttc tgagcccgcg ccggaagaat gaccttgact
      661 tccttcgaag gtttgatata tttctaatcg ggatagagta taatttatag aataagatgt
      721 ttttttactc aaaatagtgt tccgtttatt gctgtgcaat gcagggtgaa cagtctggcg
      781 acaaacaaag gaggacgaga tgatgaagtt cctgaaggca atacttgtgg tggtcttctg
      841 cctggtttcc gctggcgcct gggctttgac ggccgatgat gtcgtgtaca tgaccgagga
      901 gtaccccccc ttcaacatgg cggggtccga cggagtggcg actggagcct ccgttgaagt
      961 aatggcgggc attttcgaga gaatgggcgc ctcgaaaacg gtgaaggaca tccaggtgct
     1021 tccgtgggcg cgggggtata aagaagtcca gaatacg
//
LOCUS       DAOTHD010000932         1057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_170184_length_1057_cov_2.140719, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000932 DAOTHD010000000
VERSION     DAOTHD010000932.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170184_length_1057_cov_2.140719"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="P5219_12575"
     CDS             <1..635
                     /locus_tag="P5219_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRX27648.1"
                     /translation="GGVAKFVASTGKANFPVVAHPALFRPHFKADPVLSSKGIQTGDG
                     REAIEKAGGRLFLSADPFPLAEGLASTGQFPRVTGYEGPGKAFLTLEDGRVTPDSLPD
                     DMGITALVKGKGVVVVAGCSHSGIVNILKRVRELYPNEPVEGVIGGLHLVNGSEEKME
                     KTLEGIRDIGPKWIAAGHCTGFPMQVKLFQAFGTAFSPLCVGKKFVVEGV"
     gene            complement(735..>1057)
                     /locus_tag="P5219_12580"
     CDS             complement(735..>1057)
                     /locus_tag="P5219_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27649.1"
                     /translation="SARVKEDLAPTGATAETEELLGPPLAHFGLSDCFELYGIFSRVW
                     NAIPGQVRFRAASAPFLPPRRQPYLLPSPLRDRFTAERLAYAARLAAASGSREREERL
                     LDLA"
BASE COUNT          188 a          327 c          363 g          179 t
ORIGIN      
        1 ccggcggggt cgcgaagttc gtcgcctcca ccggaaaggc caattttccc gtggtggccc
       61 acccggccct gttccgcccc catttcaagg cggatcccgt tctctcctcc aagggaatcc
      121 agaccgggga cgggcgggag gccatcgaga aggcgggggg caggctgttc ctctccgccg
      181 accccttccc cctggccgaa ggccttgcct ccacgggaca gtttccccgg gtgacaggct
      241 atgaagggcc cggaaaggcc tttctcaccc tcgaggacgg cagggtgacc cccgactccc
      301 ttcccgacga catgggcatc accgcactgg tgaagggaaa gggtgtggtg gtggtggccg
      361 gctgcagcca ctcggggatc gtgaacattc tcaaacgggt ccgggagctg taccccaacg
      421 aaccggtgga aggcgtcatc ggaggccttc acctggtcaa cggctccgag gagaagatgg
      481 agaagaccct ggagggcatc cgggatatcg gtccgaagtg gatcgcagcg gggcactgca
      541 cgggattccc catgcaggtc aagcttttcc aggccttcgg gacggccttc tcccccctgt
      601 gcgtgggaaa gaagttcgtg gtcgaaggag tgtagtgcag aaagcgggct gtctctccgg
      661 tgtgaaaacc tccggtgccg gcgggaaccg caggacgtcg ctccccgccg gcaccggcag
      721 actggtgttt tttgtcaggc gaggtccagg agcctttcct cccgttccct ggatccagag
      781 gcggcggcga gtcgtgcggc gtatgccagc cgctcggccg tgaacctgtc cctcaggggc
      841 gacggcagaa ggtagggctg tctccgggga ggaagaaagg gagcggatgc ggcccggaaa
      901 cggacctgcc cgggaatggc gttccacacc ctgctgaaga tgccgtagag ctcgaaacag
      961 tcgctgagtc cgaagtgagc caaagggggg ccaagcagct cttccgtctc tgccgtggca
     1021 cccgtgggtg cgagatcttc cttcacccgg gcggaag
//
LOCUS       DAOTHD010000933         1056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_170353_length_1056_cov_2.913087, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000933 DAOTHD010000000
VERSION     DAOTHD010000933.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170353_length_1056_cov_2.913087"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="P5219_12585"
     CDS             <1..763
                     /locus_tag="P5219_12585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HRX27650.1"
                     /translation="LLSCSKDPVIQGERTVGAIITMRDMSELQAMAEEITGVRTYVES
                     LRVQGHEFMNKLQAISGLIQLKRYDRAIEFIATETDSRHSANAFIAERIKNAAVCGVL
                     MGKAGRCRELGIQFCLDPESFCCDRGAEINDRSLVIIVGNLLQNAIEAVEENGVRPGA
                     RIDFSIFDESGHILISVSDNAGTLTHEGASRLFEKGFTTKKKAELSGFGLYNIKTIVD
                     ALDGTISADYETGAFTEFTVSLPVAAEVPCLKEVF"
     gene            763..>1056
                     /locus_tag="P5219_12590"
     CDS             763..>1056
                     /locus_tag="P5219_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX27651.1"
                     /translation="MAPIDVLIVEDDPMVADIHQSFINSVENYRVAGVVDNGLKALEH
                     LRRHPVRLVVLDIFLPGLDGMALLEKIRESGENVDVIVVSASRDILTVNRAIQA"
BASE COUNT          259 a          286 c          301 g          210 t
ORIGIN      
        1 ccttctatcc tgcagcaagg acccggtcat ccagggggag cgcaccgtcg gagccatcat
       61 aaccatgagg gacatgtcgg aactgcaggc catggcagag gaaattaccg gggtccgaac
      121 ctacgtggag agcctccggg tccaggggca cgagttcatg aacaagcttc aggcaatttc
      181 aggccttata cagctcaaac ggtacgacag ggcaatcgaa tttatagcga ccgaaacgga
      241 ttcccgccat tcggccaacg cattcattgc ggagagaata aagaacgccg ccgtctgcgg
      301 agtactcatg ggaaaagccg ggcggtgcag ggagctggga attcagttct gcctcgatcc
      361 ggagagcttc tgctgcgaca ggggagcgga gatcaacgac cggtctctgg tgattatcgt
      421 cgggaacctt ctgcagaacg ccattgaagc tgtggaagaa aatggagtcc gaccgggggc
      481 ccgcatagac ttttccatat tcgacgagtc aggccacatc ctcatcagcg tcagcgacaa
      541 cgccggcacc ctcacccacg aaggcgcttc caggcttttc gagaaaggct tcaccacgaa
      601 gaaaaaagcg gaactttcag gattcgggct gtacaatata aaaaccatag tggatgctct
      661 cgacggaacc atctccgctg attatgaaac aggggccttt accgagttca ctgtttcatt
      721 gccggtggcc gccgaggttc cgtgcctgaa ggaggtattc tgatggctcc gatagacgtg
      781 cttatcgtgg aggatgaccc catggtcgct gacatccacc agagcttcat caactccgtt
      841 gaaaactacc gtgtcgcagg tgtggtcgac aacgggctca aagccctgga gcatctcagg
      901 cgtcaccccg tgcgccttgt ggtgctggat attttcctcc cgggccttga cggcatggct
      961 ctactcgaaa aaatcaggga aagcggggaa aacgtggacg ttatcgtggt ctccgcatca
     1021 agggacatct taaccgtcaa cagggcaata caggct
//
LOCUS       DAOTHD010000934         1055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_170569_length_1055_cov_3.766000, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000934 DAOTHD010000000
VERSION     DAOTHD010000934.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170569_length_1055_cov_3.766000"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="P5219_12595"
     CDS             complement(<1..508)
                     /locus_tag="P5219_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRX27652.1"
                     /translation="MYRYALKVYGCQMNVYDGDKMKTALKERGWTETVEEEADVVILN
                     GCSIRDKAEQKVWSDLGRFAPRWERERKPFVAVTGCVAQNVGRKMAARFPWVRFVSGP
                     RHIGAVPDGLEKLMASPGETFQFLDDDPRAFIDLPGAPVERVNPWKAFVTIAHGCDNF
                     CSYCIVPYV"
     gene            643..>1055
                     /locus_tag="P5219_12600"
     CDS             643..>1055
                     /locus_tag="P5219_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27653.1"
                     /translation="MKRFRFSGLVLPVLLIILALPGTALSWTLPDLTGFTGGEVQRTP
                     LEAPSGNFGEWLVRVYSTGGGRTLKGTLMAGPGAGELRTPPEGMKTDDRPLGFGSSCE
                     GLTVEGKRALLEEVPTVGLALAVALADDATLTLESS"
BASE COUNT          182 a          311 c          337 g          225 t
ORIGIN      
        1 ggacgtaggg gacgatgcag taggagcaga agttgtcgca gccgtgggct atggtgacga
       61 aggccttcca ggggttgacc cgctccacgg gggccccggg gaggtcgatg aaagcccggg
      121 ggtcgtcgtc gaggaactgg aaggtttctc cgggggaggc catgagcttc tccagcccgt
      181 caggcacggc gcctatgtgc ctggggcccg agacgaagcg gacccagggg aaccgggcgg
      241 ccatttttcg gcccacgttc tgggccacgc agcccgtgac ggccacaaag ggcttccgtt
      301 ctctctccca gcggggggcg aatctgccaa ggtcgctcca gactttctgt tcggctttgt
      361 cgcggatgct gcatccgttg aggatgacca cgtcggcctc ctcctcgact gtctcggtcc
      421 atccccgttc tttgagcgct gttttcattt tgtcgccgtc atagacattc atctgacagc
      481 cgtagacttt cagtgcatat cggtacacgg aagaactcct cccggcggtt ttgaattgta
      541 gacacacggt cagggagtca ttataccccc tatcggggag attgaaaaat ttggttcgcc
      601 cctgtagtag aatagaggaa aacggccgag ggggttttga aagtgaagcg ttttcgtttc
      661 tccggactgg ttcttccggt tctcctgatt atcctggccc ttccgggcac tgccctgtca
      721 tggactcttc ccgatctcac cggcttcacc gggggagaag tacagcgaac tccgcttgag
      781 gctccgtcag ggaatttcgg cgaatggctc gttcgggtct acagcaccgg cggaggccgg
      841 accctgaagg gcaccctgat ggccggcccc ggtgccggag agctccgtac tccccctgaa
      901 gggatgaaga ccgatgaccg gcccctgggc ttcggctctt cctgcgaggg actgaccgtt
      961 gaagggaaac gggctctcct ggaggaggtc cctactgtgg ggcttgccct ggcggtggcc
     1021 ctggccgacg acgcaaccct cacactcgag tcgtc
//
LOCUS       DAOTHD010000935         1055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_170588_length_1055_cov_3.043000, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000935 DAOTHD010000000
VERSION     DAOTHD010000935.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170588_length_1055_cov_3.043000"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="P5219_12605"
     CDS             <1..798
                     /locus_tag="P5219_12605"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRX27654.1"
                     /translation="ADPRLPTEIAPPVTVGMLGVIADGVDEVRKAAREQLRLGADYIK
                     VMAGGGCASAADEPDTVQYSPEELAAIVYEAKAAGKTVLTHNYSTRSMKMCAEAGVHS
                     IEHGNYLDRETARILREHGCWLVPTMATYEIMASRGEEFGIPSYFLRKMKEVQKHSEN
                     ALAIAFEEGLKIGSGSDMVGSGQPFKALEIELKARVMGPMKAILCTTRDNAELMGLSD
                     RVGTIEAGKCADFILVDGDPLKDVRIFQNREKILAIVQDGKFIKKTL"
BASE COUNT          227 a          325 c          335 g          168 t
ORIGIN      
        1 gcggatccgc gccttcccac cgagatagct cctcccgtca ccgtgggaat gctgggcgtc
       61 atcgccgacg gcgtggacga ggtgagaaag gccgccagag aacagctccg tctgggggcc
      121 gactacatca aggtcatggc cggagggggc tgcgccagcg ccgccgacga gcccgacacg
      181 gtccagtact ctcccgagga gctggcagcc attgtctacg aggcgaaggc cgctggcaaa
      241 acggtgctca cccacaacta ctccacccgg agcatgaaga tgtgcgccga agcgggagtc
      301 cacagcatcg agcacggcaa ctacctcgac agggagacgg cccggattct cagggaacac
      361 ggatgctggc tggtgcccac catggccacc tacgagatca tggcctcaag gggcgaggaa
      421 ttcggcatcc cgtcctattt cctgcggaag atgaaggaag tgcagaagca ctcggaaaac
      481 gcccttgcca tcgccttcga ggagggactg aagatcggct cgggctccga catggtgggc
      541 tcggggcagc ccttcaaggc ccttgagatc gagctgaagg cccgggtcat gggcccaatg
      601 aaggccatcc tctgcaccac ccgggacaac gcggaactta tgggactgtc cgaccgggtg
      661 ggaaccatcg aggcgggcaa atgcgccgac ttcattctcg tggacggaga ccccctgaag
      721 gacgtcagga ttttccagaa cagggagaag atcctggcta tcgtccagga cggcaaattc
      781 atcaagaaaa cgctctgagg gctgttagaa agcgcttccg ggggcggcag gatccgcccc
      841 ctcttttttt gccgcagtcc cgggcagaaa gactgtctcc ccggcttccg ccgcccaaat
      901 ccgaagcttt tgctagcaga gggagtgtcg ctgggcacag cgcccggacc gagaaaccga
      961 caaggatgtc ggtttcaggt gcagttgtca aggacgacaa tctgcaccgg cggcccaagc
     1021 gagcaccggt gacgctcccc gctgcctgcg gattt
//
LOCUS       DAOTHD010000936         1055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_170589_length_1055_cov_3.036000, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000936 DAOTHD010000000
VERSION     DAOTHD010000936.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170589_length_1055_cov_3.036000"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="P5219_12610"
     CDS             <1..860
                     /locus_tag="P5219_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27655.1"
                     /translation="LTLTVTMKYKTQNAAVSLVNTSKYLEPEAGYVIPATELKRMEVL
                     AEYWKDVKNLYPLLRQDIADGVVRWAPHPKAKWANYLESKREFNKVYAPLLQGNLFQG
                     IPFFEGKKYTELSGVVLDTEMTLRSVLAAIAGEEKNVETRRSALAQDNVLTRKEQEEI
                     RKLEAMYGPEREKNLVKAVHKAAGGYGKVVELERFVENSEMKDGAYERAKEELDTLLK
                     RRHPEQRAADSALQTFYKQLPEAQRRVDAIIADHGKRKKDLGLQPVLKEAKLAKEKTF
                     DTLKPRSEW"
BASE COUNT          282 a          253 c          344 g          176 t
ORIGIN      
        1 tcctgacgct gacggtgacc atgaagtaca agacccagaa tgccgccgtc agtcttgtga
       61 acacctcgaa gtatctcgag ccggaggcag ggtatgtcat cccggcgacg gaactcaagc
      121 ggatggaagt cctggcggag tactggaagg acgtaaagaa tctctacccc ctgctcaggc
      181 aggacatcgc ggacggagtc gtccggtggg ctccccatcc gaaggcgaag tgggcgaact
      241 acctggagtc gaaaagggag ttcaacaagg tttatgcccc gctgctgcag gggaatctct
      301 ttcaggggat tcctttcttc gagggaaaga agtatacgga gctctccggc gtggtccttg
      361 atacggagat gacccttcgc agtgtcctcg ccgccatagc cggcgaggaa aagaatgtgg
      421 aaaccaggcg gagcgctctg gcacaggata acgtcctcac caggaaggaa caggaggaga
      481 tccggaagct ggaggccatg tacggtcccg aaagggaaaa gaaccttgtc aaagcagtgc
      541 acaaggctgc cggaggatat gggaaggtcg tggaactgga gcggttcgtg gagaactctg
      601 aaatgaagga cggagcctat gaacgggcga aggaggaact ggacactctc ctgaaacgga
      661 ggcatccgga acagcgggcg gcggattcgg ccctgcagac cttttacaag cagctcccgg
      721 aagcccagag acgggtggac gccatcatag cagatcacgg aaagcggaag aaggatctcg
      781 gccttcagcc ggtgctgaaa gaagcgaagc tggcgaagga gaagaccttc gacacgctca
      841 agcccaggag cgagtggtag gtgcgtctgc ggaagggccc cgtcgagaac aatgggctga
      901 ttcgatgacc acggtgtttc aacatcatga aggaggggga tcatgagcat cttcgcacga
      961 gtttcggata tcttcaaggc gaacgtcaac gaaatactgg acaggatgga agatcctgaa
     1021 aagatggtca agcagatgat catcgagatg gaaga
//
LOCUS       DAOTHD010000937         1054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_170852_length_1054_cov_2.421421, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000937 DAOTHD010000000
VERSION     DAOTHD010000937.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170852_length_1054_cov_2.421421"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="P5219_12615"
     CDS             <1..265
                     /locus_tag="P5219_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27656.1"
                     /translation="LASVTESDLPFYYDGTYYHFEGADGEAVYYARVDEAVRDSEGRI
                     VMRGEIYNTEDKNDILGKFTALAKPHKFNGKDTWAILRMETEF"
     gene            complement(635..>1054)
                     /locus_tag="P5219_12620"
     CDS             complement(635..>1054)
                     /locus_tag="P5219_12620"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRX27657.1"
                     /translation="SPTAQPPDQDPGPLLTGAMDALWREEGRVFIRDYKITAGDENLN
                     AGEEPSWKFLYGAQLLFYGCAARSVFGEAETDIRLIRLRTGEEGAPVMPEDSWQAVEE
                     DIRSTALRAAQGPFPPRTDRCRRCFYRMDCPFRGATS"
BASE COUNT          201 a          321 c          325 g          207 t
ORIGIN      
        1 actcgccagc gtcaccgagt cggacctccc cttttactat gacggcacgt actatcactt
       61 cgaaggggcc gacggcgagg cggtgtacta tgcccgggtg gacgaggcgg tccgggattc
      121 cgaaggccgc atcgtcatgc ggggggagat ctacaataca gaagacaaga acgatatcct
      181 gggaaaattc acggccttgg cgaaacctca caaatttaat ggaaaggata cctgggcgat
      241 cctgcgcatg gagacggagt tttaatcctc cccggcagga cctgcgggac aggcctttcc
      301 gacaggaatc ccttgttcgt tcccggtccg ccgtgcggga ggacaagcac agtaacaggt
      361 cgggcgggag atccttcccg ctgaacgggc aagcgaaggg cggttctccg gaaaaggaag
      421 aaccgccctt ctactttccg gactgcttct gtttgtcatc ctgaggggcc tcatcgccga
      481 aggatccgga gttgtcattc tgagccctgg tttttcgggc gaagaatctg gttttgggag
      541 actaaaaaga gaaaaacaga tctttccctc cccccgggga tgccacgcga tcgctgtcgc
      601 ttaggatgac aatcttcccc cggttttttt tcgttcagga tgttgctccc cggaagggac
      661 agtccatgcg gtagaagcac ctccggcacc ggtcggtccg gggggggaag ggaccctggg
      721 ccgccctgag ggctgtggac cggatatcct cttccaccgc ctgccatgag tcttccggca
      781 ttacgggcgc cccttcttcc cccgtcctca ggcggatgag gcggatgtcc gtctccgcct
      841 ccccgaagac ggaccgggcg gcgcagccgt agaagagaag ctgggcaccg tagaggaact
      901 tccacgacgg ctcctcgccc gcattgaggt tttcatcgcc cgccgtgatc ttgtagtccc
      961 ggatgaacac ccgcccctct tcccgccaga gggcgtccat ggccccggtg agcagcgggc
     1021 ccggatcctg gtccggcggc tgcgccgtcg gaga
//
LOCUS       DAOTHD010000938         1053 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_171131_length_1053_cov_2.374749, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000938 DAOTHD010000000
VERSION     DAOTHD010000938.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171131_length_1053_cov_2.374749"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="P5219_12625"
     CDS             <1..246
                     /locus_tag="P5219_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HRX27658.1"
                     /translation="GGSIVTGVMLAIFMSNSGGAWDNAKKYIEEGNFGGKGTPEHAAA
                     VVGDTVGDPFKDTAGPSLNILIKLMSVVALVLAPLFV"
     gene            325..>1053
                     /locus_tag="P5219_12630"
     CDS             325..>1053
                     /locus_tag="P5219_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HRX27659.1"
                     /translation="MQQSDDVRRIKERLDIVEVIGDHVRLKKAGANFIGLCPFHDEKT
                     PSFTVSPSRQTFHCFGCGQGGDIFTFVMEKESLGFREALEVLAARAGVTLSASRGGEK
                     RDRSLSDIMEMACSHYRNLLRGPEGEAARAYLGRRNLLPETWERFELGWAPSSWDSLW
                     NSLFRQGVPPKEALDAGLVLEGKGGLYDRFRGRVIFPVRDIAGRLLAFGGRLIDGEGA
                     KYINSPEGALYSKRKNLYLLHRAKE"
BASE COUNT          214 a          305 c          345 g          189 t
ORIGIN      
        1 ggcggctcca tcgtcaccgg cgtgatgctg gccatcttca tgtccaactc cggcggtgcc
       61 tgggacaacg ccaagaaata catcgaagag ggcaacttcg gcggcaaggg aacgcccgag
      121 catgccgccg ccgtggtcgg cgatacggtg ggcgacccct tcaaggatac cgccgggccc
      181 agcctcaata ttctcatcaa gctcatgtcc gtggtggctc tcgtcctcgc gcccctgttc
      241 gtgtaagggt aaaagaagag tacaatactg gggggaaggg aaaccttccc cccgaatttt
      301 tttgtgtagt gaggaggacc aggggtgcag cagtccgatg acgtcaggcg catcaaggaa
      361 cggctcgata tcgtggaagt gatcggtgac catgtccgcc tgaagaaagc aggggcgaac
      421 tttatcggcc tgtgtccctt ccacgacgag aagaccccct cgttcacggt atctccttcc
      481 cggcagacct ttcactgctt cggctgcggc cagggaggcg atatcttcac gttcgtcatg
      541 gagaaggaga gcctaggttt cagggaggcc ctggaagtcc tcgccgcacg ggccggagtg
      601 accctttccg cctccagggg cggagagaag cgggaccgca gcctgtcgga catcatggag
      661 atggcctgct cccattaccg gaacctgctc cggggaccgg aaggggaagc cgcccgggcc
      721 tacctcggga ggaggaacct gctccccgaa acctgggagc ggtttgagct cggctgggct
      781 ccttcatcgt gggattccct ctggaacagt cttttcaggc agggagtgcc accgaaggag
      841 gccctggacg ccgggctcgt cctggaaggg aagggggggc tttacgaccg cttcaggggc
      901 agggtgatct tcccggtgag ggacattgcg ggtaggcttc tcgccttcgg gggaaggctc
      961 atcgacggcg aaggggccaa gtacatcaac agcccggaag gagcgctcta cagcaagaga
     1021 aagaacctct acctcctcca cagggcgaag gaa
//
LOCUS       DAOTHD010000939         1051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_171533_length_1051_cov_3.997992, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000939 DAOTHD010000000
VERSION     DAOTHD010000939.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171533_length_1051_cov_3.997992"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="P5219_12635"
     CDS             <1..283
                     /locus_tag="P5219_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRX27660.1"
                     /translation="LEEVISRLASRDIVLSVGDDVRSFLLDKGYQPKFGARPLRRTIQ
                     THIEDRLADFLLGKGSTREKSIEAFLDGDEVRFRSVAGQKNDLAETVSK"
     gene            323..>1051
                     /locus_tag="P5219_12640"
     CDS             323..>1051
                     /locus_tag="P5219_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRX27661.1"
                     /translation="MDGSSLWMLFLLFFIVFPQMKQQLIQWRRVTAIRNCEKRRGTRI
                     ITLIHRQETMGFLGMFSRNFINIEDSEEVLRAIRLTSDDTPIDLIIHTPGGLLLAAEQ
                     IARALKRHPSKVTVVVPHYAMSGGTLIALAADEILMDRHAVLGPVDPQIGKYPAASIL
                     KAVESKAVNDVDDETLILADVSGKALRQTRDLVYELISERLGEEKGMELAKILTEGRW
                     THDYPITVDEAADLGLPVVPNDQDS"
BASE COUNT          237 a          288 c          312 g          214 t
ORIGIN      
        1 gcttgaagaa gttatctcca ggcttgcctc cagggacatc gtcctgtcgg ttggcgacga
       61 tgtgcggtct tttctccttg acaagggata ccagccgaag ttcggggccc ggcctctccg
      121 gaggacaatc cagacccata tcgaggatag gctggctgac ttcctcctcg gaaagggaag
      181 cacacgggaa aagagcatcg aagcgtttct tgatggagat gaagtgagat ttcgctctgt
      241 cgcgggacag aaaaacgacc tggccgaaac ggtttcaaaa taggaacccc acttttcccc
      301 ttcgggcgga aggaggcatc ccatggacgg ttcatcactc tggatgttgt ttctcctttt
      361 tttcatcgtg tttccccaga tgaagcagca gctgatccag tggcgcaggg tgaccgccat
      421 caggaactgt gaaaagaggc ggggaacacg gatcatcacc ctgatccatc gccaggaaac
      481 catgggattc cttggcatgt tttccagaaa ttttatcaac atagaggact ccgaggaagt
      541 gctgagggcg atacggctta cttccgacga tacgcccatc gacctcatca tccacacccc
      601 cggcggtctc ctcctggcgg ctgaacagat tgccagggct ctgaagcggc atccgtcgaa
      661 agtgacggtg gtcgtacccc attatgccat gtcggggggc accctcatcg ccctcgcggc
      721 ggacgagatt ctgatggaca ggcacgctgt tcttggaccg gtggatccgc agatcggcaa
      781 gtatccggcg gcctccatcc tgaaagcggt ggaaagcaag gcagtaaacg acgtggacga
      841 cgagaccctg attctcgctg acgtgagcgg gaaagccctc cggcagacgc gggaccttgt
      901 gtatgaactg atatccgagc gcctcggcga ggaaaaggga atggaactgg cgaagatcct
      961 caccgagggc cggtggaccc atgattaccc gatcacggtg gacgaagcgg ctgatctcgg
     1021 tctgcctgtg gtgccgaacg atcaggattc c
//
LOCUS       DAOTHD010000940         1049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_172118_length_1049_cov_2.668008, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000940 DAOTHD010000000
VERSION     DAOTHD010000940.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172118_length_1049_cov_2.668008"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1049
                     /gene="mdoH"
                     /locus_tag="P5219_12645"
     CDS             <1..>1049
                     /gene="mdoH"
                     /locus_tag="P5219_12645"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HRX27662.1"
                     /translation="MKHCQVPRLPGKGPLGGDILSHDFVEAALMRRAGYGVWLAYDIG
                     GSWEETPPTLLDELKRDRRWCQGNLQHMRLIFTRGLFPTHRALFLNGILSYGSALLWL
                     AFLLLSSAQAVADVFFEPVYFSGTKTLFPQWPVWHPHWALTLLGSTAVLLFLPKLFSM
                     VLILVKDPQARAFGGRANLASGILLEVGLSTLLAPVRMIFHSLFVTATLLGKSVGWGT
                     QSRDDRGTSWGEAVAAHWWGTLFGAAWGGLVYLANPSFFPWISPIVASLLLSAPLSVF
                     TSRASLGKAFRNAGLLLIPEEVELPDELAGLEENLRRPEPYTPFPLSRKQGFVRAVVD
                     PLVHALHTSLLLRYK"
BASE COUNT          154 a          385 c          301 g          209 t
ORIGIN      
        1 tcatgaagca ctgccaggtg ccccgtcttc cggggaaggg ccccctgggc ggcgacatcc
       61 tcagccatga tttcgtggag gccgccctca tgcgccgcgc aggctacggc gtctggctcg
      121 cctacgacat cgggggaagc tgggaggaaa cgccgccgac cctgctggac gagctgaagc
      181 gggaccgccg gtggtgccag gggaacctcc agcacatgcg cctcatcttc accaggggac
      241 tcttccccac ccacagggcc ctgttcctca acggaatcct ctcctacgga tccgccctcc
      301 tgtggctggc cttcctgctg ttgagctcgg cccaggccgt ggcggacgtc ttcttcgaac
      361 cggtgtactt ctccggaacg aagaccctct ttccccagtg gccggtctgg catccccact
      421 gggccctcac cctgctgggg tccacggcgg tgctgctctt cctccccaag ctcttcagca
      481 tggtcctcat cctggtgaag gatccccagg cacgggcttt cggcggcagg gcaaacctcg
      541 cctcgggcat cctgctcgaa gtggggctct ccacccttct cgcccctgtg cggatgatct
      601 tccacagcct tttcgtgacc gctaccctgc ttggaaaaag cgtggggtgg ggaacccagt
      661 cccgggacga ccggggaaca tcctggggag aggcggttgc cgcccactgg tggggtactc
      721 tcttcggcgc ggcatggggc gggctggtct atttggccaa tccgtccttc ttcccctgga
      781 tatcccccat cgtcgcgtcc cttctcctgt cggcgcccct ctcggtcttc acgagccggg
      841 cctccctcgg gaaagcattc cgcaacgcag gcctgctcct catccccgaa gaagtggaac
      901 tccccgacga acttgccggc ctggaggaaa acctccggcg tcccgagccc tatacgccct
      961 ttcccctctc ccggaagcag ggatttgtcc gtgcggtggt ggatcccctt gtccacgccc
     1021 tccacacatc cctcctcctg cgctacaaa
//
LOCUS       DAOTHD010000941         1049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_172126_length_1049_cov_2.534205, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000941 DAOTHD010000000
VERSION     DAOTHD010000941.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172126_length_1049_cov_2.534205"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..>1049
                     /locus_tag="P5219_12650"
     CDS             18..>1049
                     /locus_tag="P5219_12650"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HRX27663.1"
                     /translation="MELGSLRMRNDGSAVMFSRGEDPVRIEIPAENTGGALDGDTVVA
                     RLLEPELNRGGYRGRVVEVIDRRRTEMAGIARKNSGKWFLDPLEPGLPSGIPMDTGDS
                     EDIREGDLVACSIVYGKGRLKVRLAGNVGTPDSPRALIDSVCIDSGLPDDFPEAVKTA
                     AEDAASAPEILASRRDLTGLQTITIDPADARDFDDAISIERLSDGGYKLGVHIADVSV
                     FVTPGSPLDAEAFSRGTSVYLPDRVIPMIPEVLSNGACSLQPDVERLTKTVMLEYDAE
                     GSRKGFSVFSSRIRSRKRMNYSQALEVIEGASSGDEGIDRSFQDFSALSTLLDKARMK
                     RGAVDLGASE"
BASE COUNT          229 a          282 c          331 g          207 t
ORIGIN      
        1 ccctgggagt tctcggcatg gaactcggct ccctcaggat gaggaacgac ggctcagcgg
       61 tgatgttctc caggggagag gatccggtga ggatagagat accggcggag aacaccggtg
      121 gagcccttga cggggatacc gtggtcgccc ggcttcttga gccggaactg aaccggggag
      181 gatacagggg cagggtcgtc gaggtgatag accgacgccg cacggaaatg gccggtatcg
      241 ccaggaaaaa ctccgggaaa tggttcctcg atcccctgga accaggcctt ccatcgggaa
      301 taccaatgga tacaggtgac tcagaggaca tcagggaagg cgatctggtg gcctgttcca
      361 tcgtttacgg aaagggacgg ctcaaggtga ggcttgccgg taatgtggga actcccgatt
      421 ctcccagggc actcattgac tcggtgtgca ttgactccgg ccttccggat gacttccctg
      481 aagccgtgaa aaccgccgct gaagatgccg cctccgctcc ggagatcctg gcatcacgca
      541 gggatctcac agggcttcag accattacca tcgatccagc cgatgccagg gattttgacg
      601 acgccatttc catcgaaagg ctttcagacg gcggttacaa actgggtgtt cacatcgccg
      661 acgtatccgt tttcgtcacc cctgggtcac ccctggacgc cgaggcattt tcaagaggaa
      721 caagcgttta ccttccggac agggtaatcc ccatgatacc ggaggtcctc tccaacggag
      781 cgtgttccct tcagcccgat gtggaaaggc tcacgaagac cgtgatgctg gaatacgacg
      841 cagaaggctc caggaagggt ttctccgttt tttcctccag gatccggtca aggaagagga
      901 tgaactattc ccaggcgctg gaggtgattg agggggcttc gtcgggggat gagggcatcg
      961 accggtcatt ccaggatttc tcagccctga gcactctgct ggataaagcc aggatgaaga
     1021 ggggcgcggt ggacctggga gcatctgag
//
LOCUS       DAOTHD010000942         1048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_172397_length_1048_cov_2.628399, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000942 DAOTHD010000000
VERSION     DAOTHD010000942.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172397_length_1048_cov_2.628399"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            300..>1048
                     /locus_tag="P5219_12655"
     CDS             300..>1048
                     /locus_tag="P5219_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRX27664.1"
                     /translation="MSKDVQVLWQDIIQATREESPGGAWEIWMRTCEPLEVVDGTLVL
                     DVPNVFVKEQIQSRFLEPLCDAARRQGMVKGVELRVGSEVRPAEQQRAEKAATPVPVT
                     PKNGLNPNYIFDSFVIGKSNRLAHAASLAVAESPGVAYNPLFVWGGVGLGKTHLMHAI
                     GHYLLNKQPNAKVCYVSSEKFTNELISSIKDNRTQEFKNRYRSVDVLLIDDIQFLANK
                     ESTQEEFFHTFNSLHDAKRQIVISSDRPPKE"
BASE COUNT          215 a          313 c          300 g          220 t
ORIGIN      
        1 ccgtgaccct tggcgccatc ttcaccccgt tttttcttcc acaattgttt tccccatgcc
       61 gcccggagcg gtgaatattt cgtgtggata atatttgccc cttgccggat gcggttggtc
      121 taatttgggc agattgtcgc ctattgttgg ataattcgtc aggccccttg cgtcgaaggt
      181 tcccgccttt tcacaagttc cacggaaaag gatgtggata tgtgtataac ttttgtggat
      241 gttcttgtgc tatccttttt ctgcgagtcg gaagaaacgg cttcattggg gtgatttttt
      301 tgtccaagga cgttcaggtt ctctggcagg acatcatcca ggccacaagg gaggaatctc
      361 ccgggggggc ctgggaaata tggatgcgca cctgcgaacc cctggaggtc gtcgacggca
      421 cgctggttct cgacgtgccg aacgttttcg tcaaggaaca gatccagtcc cgcttcctgg
      481 aacccctctg cgacgcggcc cgcaggcagg gaatggtgaa gggcgtcgag ctccgggtgg
      541 gctcggaagt ccggccggcc gagcagcagc gggcagagaa ggcggcgacg cccgtccccg
      601 tcacgccgaa aaacgggctg aacccgaact acatcttcga cagcttcgtc atcggcaagt
      661 ccaaccggct ggcccacgcg gcgagcctgg cggtggccga gtcccccggg gtggcctaca
      721 acccgctttt cgtctgggga ggcgtggggc tgggcaagac ccacctcatg cacgccatcg
      781 ggcactacct cctgaacaag cagcccaacg cgaaagtctg ctacgtgagc tcggaaaagt
      841 tcacgaacga actgatttcc tccatcaagg acaaccggac gcaggaattc aagaaccggt
      901 accgttcggt ggacgtcctc ctgatcgacg acatccagtt cctggccaac aaggaaagca
      961 cccaggagga attcttccac accttcaaca gcctgcacga cgccaagcgg cagatcgtca
     1021 tcagctccga ccgccctccc aaggagat
//
LOCUS       DAOTHD010000943         1048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_172430_length_1048_cov_2.345418, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000943 DAOTHD010000000
VERSION     DAOTHD010000943.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172430_length_1048_cov_2.345418"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="P5219_12660"
     CDS             complement(<1..262)
                     /locus_tag="P5219_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27665.1"
                     /translation="MKLWNTNPLHGIRLTGGRGSTVFDDRGRSYTDMWSGTWCNVLGY
                     GHPRLARALRDQAGGLLQAGASFGAPEMDDALRELEEEIGRGA"
     gene            complement(267..>1048)
                     /locus_tag="P5219_12665"
     CDS             complement(267..>1048)
                     /locus_tag="P5219_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRX27666.1"
                     /translation="DELGHRLTGIRMDLDALENTCRKGPLHLVYTMPNFQNPTGITTD
                     QPHRERLLDICSRFGVPLLEDAFEEEMKYSGRVVLPVKSMDTGHRVIYCGTFSKVLFP
                     GARVGWLAAHPECIRRLAVLRRYGELSPPALLQAALARFCSEGHYERHISRMHRVYRK
                     RMQTALAALNRAVSPEWATWTRPDGGYLIWLSMVPSPPADWEKILASEKVSAAPGYTF
                     YSSEPTGVHFRLSISSLDEEEIEEGIRRLGRAFGKVYERKG"
BASE COUNT          167 a          337 c          344 g          200 t
ORIGIN      
        1 gcgcgccgcg gccgatctct tcttcgagtt ctcggagggc gtcgtccatc tcgggagccc
       61 cgaaggatgc ccccgcctgc agaagccccc ctgcctggtc ccggagggcc cgggccagcc
      121 ggggatggcc gtacccgagg acgttgcacc atgtgcccga ccacatgtcg gtgtagcttc
      181 tgccccgatc gtcgaagacg gtgcttcccc tgcctcccgt caggcggatg ccgtgcaggg
      241 ggttcgtgtt ccagagtttc atctcctcat cccttccttt catacacctt cccgaaggcc
      301 cttccgagtc tgcggatgcc ttcttcgatc tcctcctcgt ccagggacga gatggagagg
      361 cggaaatgga cccctgtcgg ttccgaggag tagaaagtgt accccggggc ggcgctgacc
      421 ttctccgagg ccaggatttt ctcccagtcc gccggcgggg agggaaccat ggaaagccag
      481 atgaggtatc ctccgtccgg ccgggtccag gttgcccatt ccggggagac agcgcgattc
      541 agcgcagcca gggcggtctg catccgtttc ctgtagaccc ggtgcatcct tgagatatgc
      601 cgttcgtaat gcccttcgga gcagaagcgg gccagggctg cctgcagcag cgccgggggc
      661 gacagttcgc cgtaccggcg gagcacggcc aggcgccgga tgcattccgg gtgggccgcc
      721 agccacccca cgcgggcccc ggggaagagt acctttgaaa aggtaccgca gtagatcacc
      781 cggtggccgg tgtccatgga cttgacggga agcaccaccc tgcccgagta tttcatttcc
      841 tcctcgaagg cgtcctccag aaggggcacc ccgaagcgcg aacagatgtc cagcaggcgc
      901 tcccggtggg gctggtccgt ggtgatcccc gtggggttct ggaagttcgg catggtatag
      961 accaggtgga ggggaccctt ccggcaggtg ttttccaggg cgtcgaggtc catgcggatt
     1021 ccggtgagtc gatgtcccag ttcatcgt
//
LOCUS       DAOTHD010000944         1046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_173055_length_1046_cov_1.702321, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000944 DAOTHD010000000
VERSION     DAOTHD010000944.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173055_length_1046_cov_1.702321"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1046)
                     /locus_tag="P5219_12670"
     CDS             complement(<1..>1046)
                     /locus_tag="P5219_12670"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRX27667.1"
                     /translation="DFLSSVPHLDSLLGTKDPELIENAFLATPIPDYAVRTVERYLSR
                     MTDPVVVRSSSRLEDSLKHSFAGKYMSTFLINGPDQPLSHRVDAVLEQMKRIYARNYF
                     PTAASYRRKHGLPEDEMGLILMRMAGKWRGRYFYPAMAGVGFSRNYRRWTTRIRAEDG
                     VLRIVFGLGTLSTKRGYARTFSLSNPMLRPEGLNPFNIMRHSQEAFQAVGQEEGQLLT
                     LNITRSWKDIIPHVPNFRLYAQIYNPDNFGGYFTYIGSFPSFQGESSKICFTFEDFPC
                     RHRRFFEIIKGVLPFLEESMGVPADLEFAYEPVDEKLQLLQCRPLWGGEASGTGTKPV
                     LEDRKILFKADRMV"
BASE COUNT          221 a          276 c          313 g          236 t
ORIGIN      
        1 gaccatcctg tccgctttga aaaggatctt gcggtcctcc aggacgggtt ttgtgcccgt
       61 gccggaggcc tctccccccc aaaggggcct gcattgcagt agctggagtt tctcgtccac
      121 cggctcgtag gcgaattcaa ggtccgccgg aacgcccatg gattcttcaa ggaaaggcag
      181 gacgcctttt atgatttcaa aaaaccttcg atgacggcat gggaaatcct cgaaagtgaa
      241 gcagatcttt gaggattcgc cctggaaaga aggaaaactg ccgatgtagg tgaagtagcc
      301 cccaaagttg tccgggttgt aaatctgggc gtaaagccta aagttcggga catgagggat
      361 gatgtctttc caggaacggg tgatgttcag ggtgagaagc tggccttctt cctgccctac
      421 ggcctggaag gcttcctgtg aatggcgcat gatgttgaaa gggttcaggc cttccggcct
      481 gagcatgggg tttgacaggg aaaaggtcct tgcgtagccc ctcttggtgc tgagggtccc
      541 caggccgaac acgatcctga gcactccgtc ttccgccctt atgcgggttg tccatctcct
      601 gtagttcctc gagaacccca cgccggccat ggccgggtag aaatacctgc ccctccattt
      661 gccggccatc cgcatgagga tcaggcccat ctcgtcctcg ggaagcccgt gtttcctcct
      721 gtagctcgcg gctgtaggga aatagttccg agcatagatc ctcttcatct gctccaggac
      781 ggcgtccaca cggtgggaca ggggctggtc ggggccgttg atgaggaaag tggacatgta
      841 tttcccggca aaggaatgct tgagcgaatc ttccagcctg gaactgcttc tcacgacaac
      901 ggggtccgtc atcctcgaga gatatctttc cacggtcctt acagcatagt cgggaatagg
      961 agtcgcaagg aaagcgttct cgatgagctc tggatccttc gtgccgagaa gggaatccag
     1021 gtggggtacg gaggaaagga agtcgt
//
LOCUS       DAOTHD010000945         1045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_173152_length_1045_cov_3.164646, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000945 DAOTHD010000000
VERSION     DAOTHD010000945.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173152_length_1045_cov_3.164646"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1045)
                     /locus_tag="P5219_12675"
     CDS             complement(<1..>1045)
                     /locus_tag="P5219_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRX27668.1"
                     /translation="EVEELHRGKVVTGTVVNAVDGGWLVDVGYKCEGFLPAKEWSHKV
                     LVEDSEEPAPGDKIQIQVVSIRHGEEAQLLVSRWRCEFDRRWSELENILAQNDVVSVT
                     GLRKVKGGLMVECCGLEGFVPISHLAEEGRGVNPGKFVGEVFEAKLMEKDKKKHRLVF
                     SRRLIVEESLVEEREKFYTDVQEGTVLDGEVSSITSFGVFVNVGPLDGLVHMSELSWK
                     RNIKPKEMFKKGDPVKVKVIGIDREANRLSLSMKQTKEDPWMSAADRWKPQDKARGVV
                     TNVTDFGAFVEVEPGIEGLIHIGDLSWTRIKHPRDVLRKGQEVDVVVLDVDMEKKRMS
                     LGYKQLNDPWNDVL"
BASE COUNT          183 a          354 c          270 g          238 t
ORIGIN      
        1 agagaacgtc gttccagggg tcattgagct gcttgtaccc caggctcatg cgctttttct
       61 ccatgtccac gtcaaggacc accacgtcaa cttcctggcc cttccggagc acgtcccggg
      121 gatgcttgat cctggtccag ctcaggtcgc cgatgtggat aaggccctcg atgccgggct
      181 ccacttccac gaaagcgccg aagtcggtga cgttggtcac cacacccctg gccttgtcct
      241 ggggcttcca gcggtccgcg gcgctcatcc aggggtcttc cttggtctgt ttcatgctca
      301 gggaaagccg gttggcctcc cggtcgatcc cgataacctt caccttcacg gggtcgccct
      361 tcttgaacat ctccttgggc ttgatgttcc gcttccagga gagctcgctc atgtggacaa
      421 ggccgtcgag gggacccacg ttcacgaaca cgccgaagga ggtgatgctg ctcacttcgc
      481 cgtcgagaac cgttccttcc tgaacgtcgg tgtagaactt ctcccgctcc tccaccaggg
      541 attcctccac gatgagcctg cgggagaaga caaggcggtg cttcttcttg tccttctcca
      601 tcaatttagc ttcgaagact tcgcccacga atttccccgg attcacaccc cggccttcct
      661 cggcgagatg tgagatgggc acgaagccct ccaggccgca gcattccacc atgagcccgc
      721 cctttacctt gcgcaggccc gtcacggaaa ccacgtcgtt ctgggcaagt atattttcaa
      781 gttcactcca gcgccggtca aactcgcacc gccatctgct cacgagaagc tgggcctctt
      841 cgccgtggcg gatgctgacc acctggatct gtattttgtc tccaggagcc ggttcttccg
      901 aatcttccac gagaaccttg tgagaccatt ccttcgcggg caggaatccc tcgcacttgt
      961 agcccacgtc cacgagccag ccgccgtcaa cggcgttcac gaccgttccg gtgaccacct
     1021 tgccccggtg aagctcttca acttc
//
LOCUS       DAOTHD010000946         1044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_173406_length_1044_cov_3.294237, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000946 DAOTHD010000000
VERSION     DAOTHD010000946.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173406_length_1044_cov_3.294237"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="P5219_12680"
     CDS             <1..553
                     /locus_tag="P5219_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HRX27669.1"
                     /translation="TVLELGRIALSHARAGADMVAPSAMMDGQVAAIRRALDNGGFPS
                     LPVMSYSSKFSSAFYGPFRDAAGSAPSFGNRRSYQMASTNWKEALRESLLDEEEGADI
                     LMVKPSLLYMDILSRLRESTLLPLACYLVSGEYMMLRHAAAAGSLDGPRGLLEAHLAL
                     RRAGADILITYAAVEVARMVTGR"
     gene            complement(540..>1044)
                     /locus_tag="P5219_12685"
     CDS             complement(540..>1044)
                     /locus_tag="P5219_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRX27670.1"
                     /translation="DLTCLGKIIGGGLPVGAVGGRREVMEVLAPLGPVYQAGTLSGNP
                     LAMASGLATLGVLKREGVYEQLEERAVLFADGLKDAASHAGIPLSVSRFGSVLGLFFA
                     PRLPRNLGEVKGTDGAKYPPFFHGMEERGQYFAPSPFEAAFVSLAHGREVVEETLAAA
                     GEVFTSL"
BASE COUNT          173 a          389 c          330 g          152 t
ORIGIN      
        1 cacggttctc gagctcggcc gaatcgccct cagccacgcc cgggccggtg cggacatggt
       61 ggccccctcc gccatgatgg acggccaggt ggccgccatc cggagggccc tggacaacgg
      121 gggattcccc tccctgccgg tgatgagcta cagctccaag ttctcgtcgg ccttctacgg
      181 tcccttccgg gacgcggccg gcagcgcccc ctccttcggg aaccggcggt cctaccagat
      241 ggcctccacc aactggaaag aagccctccg ggagtccctt ctcgacgagg aggaaggggc
      301 cgacatcctc atggtgaaac cctccctcct ctacatggac attctttccc ggctccggga
      361 gtccaccctg ctgcccctgg cctgctacct ggtgagcggc gagtacatga tgctccggca
      421 cgcggccgcc gccggttccc tggacggtcc aagggggctc ctggaggccc acctcgccct
      481 ccgcagggcg ggagcggaca tcctgatcac ctacgccgcc gtggaagtgg cccgaatggt
      541 cacagggagg taaagacctc cccggcggcg gcgagagtct cctccaccac ctcccggccg
      601 tgggccaggg agacgaaggc ggcctcgaaa ggcgacggcg cgaagtactg ccccctctcc
      661 tccatgccgt ggaagaaggg agggtatttc gccccgtccg tccccttcac ctccccgagg
      721 ttccggggaa gccggggagc aaagaacagc cccagcaccg agccgaaccg ggagaccgac
      781 agggggatcc cggcatggga agcggcatcc ttcagcccgt cggcgaacag gacggccctc
      841 tcctccagtt gctcgtaaac cccttcccgc ttcagcaccc cgagggtcgc cagtcccgac
      901 gccatggcca ggggattccc cgaaagggtt ccagcctggt acaccggccc gaggggggcg
      961 agcacctcca tgacttcccg gcggccgccc acggccccca cgggaaggcc cccgccgatg
     1021 atctttccga ggcaggtgag gtcg
//
LOCUS       DAOTHD010000947         1043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_173683_length_1043_cov_3.698381, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000947 DAOTHD010000000
VERSION     DAOTHD010000947.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173683_length_1043_cov_3.698381"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..431)
                     /locus_tag="P5219_12690"
     CDS             complement(42..431)
                     /locus_tag="P5219_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27671.1"
                     /translation="MIKPYSLQRFRASLENYLEHRMRLRDVREETTQEEVDAFFEIGH
                     RPLGQPEDDETLPKGFQKETLALVEGILGESGSPTALELGEKLSISRSTARRYLEYLA
                     KKNRAEISFEYRTSGRPLKRYTAMGKK"
     gene            complement(428..748)
                     /locus_tag="P5219_12695"
     CDS             complement(428..748)
                     /locus_tag="P5219_12695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX27672.1"
                     /translation="MDVQLPVRVLIAEDDPMVCSLHVQAVRQLTGFTVAGTVGDGYEL
                     LEFFQRNSADLIVMDIFMPRLSGLEALKRLRSRGNTSDVILVSAGKKPISCHGRVLSE
                     PSIT"
BASE COUNT          206 a          294 c          285 g          258 t
ORIGIN      
        1 cgttcgctct gtgacctttt ttgaagggcg gcggcgttca gtcatttttt gcccatggct
       61 gtgtagcgtt tgaggggccg tccgctggtc cggtattcga agctgatttc cgcacgattt
      121 tttttagcga ggtattccag atatcgccgg gccgttgagc gggagatgga aagcttttct
      181 ccaagttcga gggccgttgg gctgccggat tccccgagga tcccctccac cagagcaaga
      241 gtttccttct ggaatccctt ggggagggtc tcgtcgtcct caggctgtcc taagggccta
      301 tgcccgattt cgaagaaagc gtccacttct tcctgggttg tttcctccct gacgtcccgg
      361 aggcgcatcc ggtgctccag gtagttttcg agggaagccc tgaatctctg gagggaatag
      421 ggcttgatca tgtaatcgaa ggctccgaga gtacgcgccc gtgacacgag atcggttttt
      481 ttcctgcgga aacaaggatc acgtcggatg tgttccccct ggacctgagt ctcttgagag
      541 cttcaaggcc ggagaggcgg ggcatgaaga tatccatgac gatcaggtcg gcgctgtttc
      601 gctgaaagaa ttcaagaagc tcgtagccgt caccgactgt tccggcgacg gtgaaacccg
      661 tcaactggcg tactgcctga acgtgaagac tgcacaccat aggatcgtcc tctgcgatga
      721 gtacccgcac gggaagctga acatccacca cgcatcctcc cccttcgatc tcattatatt
      781 ccctgaactg ccgagcgtaa acaatatgaa caaaatgacc tgaatgccca atagtcttgg
      841 ctttttttat attagggccg aaaatacaac tacaaaacac gatcacttcg ttcggttgat
      901 gaacttcccc acgcacgggt cccgcaaggc gaacggtccg ccccagacct tcgttttgcg
      961 gggcctcctt tttttctcct cccctccttc ctaaatccgc aggcagcggg gagcgtcacc
     1021 ggtgctcgct tgggccgccg gtg
//
LOCUS       DAOTHD010000948         1043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_173783_length_1043_cov_2.293522, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000948 DAOTHD010000000
VERSION     DAOTHD010000948.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173783_length_1043_cov_2.293522"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="P5219_12700"
     CDS             complement(<1..738)
                     /locus_tag="P5219_12700"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712825.1"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HRX27673.1"
                     /translation="MTISPLAGHRPPHTMLASIPGLVSAYYTEQPDPENPLQLVSFGT
                     SGHRGSSLKGSFNEAHILAVSQAIAEFRTGKGINGPLFVGKDTHALSEPAFRTAVEVF
                     AANGVDTAVQEGGGYTPTPVISHAILGWNRGRTSGLADGVVITPSHNPPEDGGFKYNP
                     PSGGPADPEMTDRIQARANEILRSGGKGVRRIPFSSAMKSDTVRAMDYIAPYVNDLGA
                     ILDLQAIARSGVSIGADPMGGSGVAYWD"
     gene            complement(749..>1043)
                     /locus_tag="P5219_12705"
     CDS             complement(749..>1043)
                     /locus_tag="P5219_12705"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRX27674.1"
                     /translation="FLGTVCTGAFIAAEAGLLEGRAATTHHGFFDELDAYPGVKVMEK
                     KVVKDGRILTAGGVSSGIDLSLWILALLFDRDFAAEAAGYIEYPFRPDVFFEK"
BASE COUNT          196 a          310 c          355 g          182 t
ORIGIN      
        1 gtcccagtag gccacgccgg agccgcccat gggatccgcg ccgatggaga cgcccgaccg
       61 ggcgatggcc tggaggtcaa gaatggcccc gaggtcgttt acgtaggggg caatgtaatc
      121 catcgccctg accgtgtcgg atttcatcgc cgaagagaag ggaatcctcc ggacgccctt
      181 tcctcccgac ctcaggattt cgttggcccg ggcctggatc cggtcggtca tttccggatc
      241 ggcgggcccc cccgacgggg ggttgtactt gaagcccccg tcctccgggg ggttgtggga
      301 cggggtgatg actacaccgt cggcgagacc ggatgtccgg cccctgttcc atcccaggat
      361 ggcatgggaa atcacgggag tgggtgtata ccctccccct tcctggactg ccgtgtccac
      421 gccgttggcg gcaaaaacct ccacggcggt ccggaaagcc ggctccgaga gagcatgggt
      481 atcttttcca acgaacaggg gaccgttgat ccccttccct gtcctgaact cggcgatggc
      541 ctgggaaacg gcgagaatgt gggcctcgtt gaaggacccc ttcagggaag atcccctgtg
      601 ccccgacgtg ccgaaggaga cgagctggag gggattctcc gggtcgggct gttccgtgta
      661 gtaggccgac accaggccgg ggatggaggc gagcatggta tggggcggcc ggtgacccgc
      721 aagaggagag atcgtcatga aaagcctcct atttctcgaa aaaaacgtcg ggacggaagg
      781 gatactcgat gtagcccgcc gcctcggcgg cgaaatcacg gtcaaagagc agggcgagaa
      841 tccacaggga aaggtcgatc cccgaactga cgccgccggc ggtgagaatg cggccgtcct
      901 tcaccacctt tttctccatg accttgacgc cgggataggc gtcgagttcg tcgaaaaagc
      961 cgtggtgggt agtggctgcc cttccctcga ggaggccggc ctcggcggcg atgaaggcgc
     1021 cggtgcagac tgtgcccagg aag
//
LOCUS       DAOTHD010000949         1041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_174185_length_1041_cov_3.885396, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000949 DAOTHD010000000
VERSION     DAOTHD010000949.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174185_length_1041_cov_3.885396"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..693
                     /locus_tag="P5219_12710"
     CDS             172..693
                     /locus_tag="P5219_12710"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRX27675.1"
                     /translation="MRILDAVKNSLTWKFILWLQRALLVLSSLFVVLIICVTVLLRYV
                     FKSDLFGMEEIVVIAAFWLYFMGSSYGVYDQSHVKADIIPQLLPPRGRAILAVFMDLV
                     MAVLCILFTVWAIDMVLYSIEWMPRTTGLRIPVFISQSSVLAGYALMSFYAVVHFFDG
                     LFSFLSGAQEGTS"
     gene            765..>1041
                     /locus_tag="P5219_12715"
     CDS             765..>1041
                     /locus_tag="P5219_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRX27676.1"
                     /translation="MAITIALLLIIITLLLGLPVPFCFMLSTTFMVITHGYDPSFLLP
                     YGFSQMSSVVLLAIPLFIMVGGMMDRGGIGTSLVNFVDVFVGWIKGGL"
BASE COUNT          202 a          277 c          278 g          284 t
ORIGIN      
        1 atcccggaaa aggaaatggc cgagcttgca gaaaccatcc ggaacaccac gtggcccaaa
       61 ctggagaaga cctatggaaa ggaaacactt gataaaataa aggaatcgct gcagtaaaac
      121 actcttcggg ccgggcggcc tttgctgccc ggcccgtctg gagggatccc attgagaatt
      181 ttggacgcag tcaagaattc ccttacctgg aaatttatcc tctggcttca gcgcgccctt
      241 ctggtgctga gcagtctttt cgtcgtcctt atcatatgcg tgaccgtgct gctgcgttac
      301 gtattcaagt cggacctttt cggtatggag gagatcgtcg tcatcgctgc cttctggctc
      361 tatttcatgg gaagctccta tggcgtctat gaccagagcc atgtgaaggc ggacattatc
      421 ccccagcttc ttcctcccag gggaagagcg atccttgcgg ttttcatgga ccttgtcatg
      481 gcagtgcttt gtattctgtt caccgtatgg gcgattgaca tggttttgta cagcatcgag
      541 tggatgcccc ggacgacggg gctacgaata ccggtgttca tttcacagtc ctcggttctc
      601 gccggctacg cgctcatgtc cttctatgcg gtggtccatt tttttgacgg ccttttcagc
      661 tttttgtccg gagcgcagga gggaacgtcc tgagctgaag tctaccccgg tcaggctccg
      721 gagggaacat aacggacctc ggccgaaatc tggaggacac gcctatggca attaccattg
      781 cacttttgct tattatcatt acccttcttc tcgggctgcc ggttcctttc tgttttatgc
      841 tctccaccac gttcatggtg ataacccacg gctacgaccc gtcgttcctc cttccctacg
      901 ggttcagcca gatgagttcc gttgtgcttc tcgccatccc gctgtttatc atggtcggcg
      961 gaatgatgga caggggaggc atcggcacgt cccttgtgaa tttcgtcgat gtatttgtcg
     1021 ggtggattaa gggaggactg g
//
LOCUS       DAOTHD010000950         1041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_174301_length_1041_cov_2.176471, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000950 DAOTHD010000000
VERSION     DAOTHD010000950.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174301_length_1041_cov_2.176471"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="P5219_12720"
     CDS             complement(<1..546)
                     /locus_tag="P5219_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRX27677.1"
                     /translation="MALYRAHVLICHGTGCASGGSPKVLETFREELQKKNLDKEVLVI
                     ATGCHGMCEFGPIVVVYPEGTFYSQVKPEDVAEIVEEHLYKGRIVNRLLYKEPISAQK
                     VCHYKEIPFYGKQHRIVLSNCGYINPDNIDEYISRDGYQALGKCITEMSPAAVIEEMK
                     KSGLRGRGGGGFSTGLKWSFCV"
     gene            complement(565..945)
                     /locus_tag="P5219_12725"
     CDS             complement(565..945)
                     /locus_tag="P5219_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HRX27678.1"
                     /translation="MTEKKIRSLDDLRKLKEKTGDLTSARSEDDKVKIILGMGTCGIA
                     AGAREIMNAVLKELETRKLHNVAVETTGCIGMCQKEPLLDVIRPGEPRITYCLVTPAD
                     VTRIISEHVVNGRIVQDKLFGRAD"
BASE COUNT          188 a          287 c          277 g          289 t
ORIGIN      
        1 cacgcagaaa ctccacttga gacctgtgga gaaaccgccg ccgccgcgcc cgcgaaggcc
       61 cgattttttc atttcctcga tgaccgctgc gggagacatt tcggtgatgc attttcccag
      121 ggcctggtat ccgtccctgg aaatatactc gtcgatgttg tccgggttga tatagccgca
      181 gttgctgagg acaattcgat gctgtttccc gtagaagggg atttccttgt aatgacatac
      241 tttctgtgct gaaatgggtt ccttgtagag aagccggttg actatgcgcc ccttgtagag
      301 atgctcttcc actatttcgg cgacatcctc gggcttgacc tgcgaataga aagttccttc
      361 cgggtagacg acgacgatgg ggccgaattc gcacattccg tggcagccgg tggcgatgac
      421 caggacttcc ttgtcgaggt tcttcttctg cagctcttcc cggaaggttt cgagtacctt
      481 cggcgatcct cccgaagcgc agcctgtccc gtggcagatg agaacgtgtg ctctgtataa
      541 agccattgtc ttctcctccc cgatttagtc agccctgccg aagagcttgt cctggacaat
      601 gcggccgttg actacatgtt ctgaaattat ccgtgtcaca tcagccggcg tgacgaggca
      661 ataggtgatt ctcggttcgc cggggcggat cacgtcgaga agcggttctt tctggcacat
      721 gccgatgcag ccggttgtct ccaccgctac attgtggagt tttctcgttt cgagttcctt
      781 caggacggcg ttcatgattt cccgcgcccc ggcagcgatg ccgcatgtcc ccattccgag
      841 gatgatcttt accttgtcat cttcggaacg ggcggacgtc aggtctccgg ttttttcctt
      901 gagcttccgg aggtcgtcca ggctacgaat ctttttttcg gtcattcatt tcacccctgt
      961 gatcagaatg gtatttccag gctactcgta cagttcatcg atgttggttt cgatatattc
     1021 ctttactccc agggcgagcg c
//
LOCUS       DAOTHD010000951         1040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_174536_length_1040_cov_2.495431, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000951 DAOTHD010000000
VERSION     DAOTHD010000951.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174536_length_1040_cov_2.495431"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /gene="aroD"
                     /locus_tag="P5219_12730"
     CDS             <1..713
                     /gene="aroD"
                     /locus_tag="P5219_12730"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HRX27679.1"
                     /translation="CVPLVGKTRGEILAEAENIPAIAPDIIELRVDAWEFVEETAASV
                     SMIRDVRKAVGEIPVILTCRGHWEGGFKKVSDEAKFSLYREAAAGGLVDFLDIELAYG
                     DEKIRETLRMLENSSVSLIVSFHDFEKTPPKEVLFSTLAAEIRAGAHVAKLAAMPRNE
                     EDVLALLSATLLVRREYPHIPLITMSMGGIGAVSRVTGGLFGSDLSFAVGSKASAPGQ
                     IPVAALKQCLSVLHPLES"
     gene            743..>1040
                     /locus_tag="P5219_12735"
     CDS             743..>1040
                     /locus_tag="P5219_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRX27680.1"
                     /translation="MMKKDLVAFQLVKFLEARGVEKIFGLCGHTVIGFLDALKESSIH
                     FVSVRHEQIAAHAADGYSRGKGCRVPGVLLTHLGPGLANATTGVAEASLNSIPMV"
BASE COUNT          197 a          324 c          307 g          212 t
ORIGIN      
        1 tctgcgtccc ccttgtcgga aagacccgcg gggaaatcct ggccgaagcg gaaaatattc
       61 ccgccatcgc ccccgatatc atcgagctgc gggttgatgc ctgggagttc gtggaagaga
      121 cggccgcctc cgtctccatg atacgggacg tccggaaggc cgtgggggag atccccgtga
      181 tcctcacctg tcggggccac tgggagggcg gtttcaaaaa agtctccgat gaggcgaaat
      241 tctccctcta ccgggaggca gccgccggag gcctggtgga ttttcttgac attgagctcg
      301 cctacggcga cgaaaaaatc agggaaaccc tccggatgct tgagaattcc tctgtttccc
      361 ttatcgtctc attccatgat tttgaaaaga cccctccgaa ggaagtcctt ttttccaccc
      421 tcgccgccga gattcgggca ggggcccacg tggcaaagct tgccgccatg cccaggaacg
      481 aggaggatgt ccttgccctt ctttccgcaa cgctcctggt ccgccgggag tacccccaca
      541 tcccgctcat caccatgtcc atgggcggca tcggcgccgt gagcagggtg accggagggc
      601 tgttcggatc ggatctttcc ttcgcggtgg gcagcaaggc ctcggccccc ggccagatac
      661 cggttgcggc cctgaaacag tgcctgtctg ttctccatcc cctggagagc tagacggtac
      721 agaaaaaaga atggaggctc ggatgatgaa gaaagacctt gtggcgttcc agcttgtgaa
      781 gtttctcgag gcccgcggcg ttgaaaagat tttcggcctg tgcggccaca ccgtcatcgg
      841 tttcctcgat gcgctcaaag aaagctccat ccatttcgtg tccgtccggc atgagcagat
      901 tgccgcccat gcggccgacg gctattccag gggaaaggga tgccgtgttc ccggtgtgct
      961 cctcacccac ctcggccccg ggctcgccaa cgccaccacg ggcgtagcgg aggcctcgct
     1021 gaactcaatc cccatggtgg
//
LOCUS       DAOTHD010000952         1039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_174747_length_1039_cov_3.079268, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000952 DAOTHD010000000
VERSION     DAOTHD010000952.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174747_length_1039_cov_3.079268"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="P5219_12740"
     CDS             complement(<1..210)
                     /locus_tag="P5219_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRX27681.1"
                     /translation="MGGLVRFCIKRPVFTWAMVLILVLLGVSSYNKLAVSLLPKVDIP
                     VVVVQTKYTGASPNEIEQLVTKPIED"
     gene            complement(213..>1039)
                     /locus_tag="P5219_12745"
     CDS             complement(213..>1039)
                     /locus_tag="P5219_12745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRX27682.1"
                     /translation="KQSLLKQQSAKAGQVLLTLSSQDYRASAAADRVSYEDAVRDYKR
                     LSELHKSGGVSKAQVEQAFSRMKSEEAKLQSSRSTLARTQIRASIDGIVASRSVEPGE
                     VASDGKALLTIIDLKDLEAEIMVSRKDILSLNKDTPVEVLSDGQKTKCSIKRISPEAA
                     PGSGLYPVVVCLNGLDVLPGTHVEARFLVDKKKDLIIIPSEIVQRRGDKAYVYVVDSE
                     RAKLREIVPGEGQNGSVSIENGLSEGDLVVVRGHNLLFENALVALTGEAAAGTAGK"
BASE COUNT          193 a          328 c          296 g          222 t
ORIGIN      
        1 atcctcgatg ggtttcgtga ccagctgttc gatctcgttg gggctggcgc cggtatactt
       61 cgtctggaca acgaccacgg gaatatcgac cttgggcagc agggaaacgg caagcttgtt
      121 gtagctgctc accccgagga ggacgagaat aagcaccatc gcccaggtga acaccgggcg
      181 ttttatgcag aaacgtacaa gacctcccat ggctacttgc ctgccgtacc ggcggcggcc
      241 tcccctgtca gagcgacgag ggcgttctca aaaagaagat tatgccccct gacgaccacc
      301 aggtctcctt ccgaaagccc gttttctatg gaaaccgagc cgttctgccc ctcgccggga
      361 acgatctccc tcagtttcgc ccgctcgctg tcgaccacgt agacatatgc cttgtcaccc
      421 cttctctgga cgatttccga ggggatgatg atcaggtcct ttttcttatc cacgaggaag
      481 cgggcctcca catgggttcc gggaagcacg tcaaggccgt tcaggcagac caccacggga
      541 tacaggcccg accccggggc tgcttcgggg ctgatcctct tgatggaaca cttcgtcttc
      601 tgcccgtcgc tcagcacctc cacgggagta tccttgttca ggctcaggat gtccttccgg
      661 gaaaccatga tctccgcctc aaggtccttc aggtcgataa tggtcaacag tgcctttccg
      721 tcggaagcaa cctccccggg ctccacggaa cggcttgcca cgatgccgtc gatgctggcc
      781 cggatctggg tgcgggcaag ggtggaccgg gaggactgga gctttgcttc ctcgctcttc
      841 atcctggaaa aagcctgctc cacctgggcc ttggaaacac cgcccgattt gtggagttct
      901 gaaagccgtt tgtagtcccg caccgcgtcc tcgtagctca ccctgtcggc ggcggcggac
      961 gcgcggtagt cctgggagga gagggtcagc agcacctgcc ccgccttggc actttgctgc
     1021 ttcaacaggg actgcttca
//
LOCUS       DAOTHD010000953         1039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_174803_length_1039_cov_2.439024, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000953 DAOTHD010000000
VERSION     DAOTHD010000953.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174803_length_1039_cov_2.439024"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="P5219_12750"
     CDS             <1..516
                     /locus_tag="P5219_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27683.1"
                     /translation="GWGSPPSPGTIAPGASVTVTAFAMGDAEGVAVSFAGGGTSLGSL
                     TALSVHSTEKSADGTYRGAVTNIGKGTISLTVSGRSARAVITGHYTADGQNVKISAGG
                     SGTYNPRKGTVSLKWAGTSNGYMLDDGERYDVNERVSGTLSGSFHKGTFSGKWWGGSE
                     FVILSGEWSAR"
     gene            536..>1039
                     /locus_tag="P5219_12755"
     CDS             536..>1039
                     /locus_tag="P5219_12755"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRX27684.1"
                     /translation="MRMRFLVYLAAGYVCLVLAASIFAGSLIFFPRRELEGNPSERGL
                     PFEDVSFRAGDGTSLHGWFLPGKDRGTVLFCHGNAGNISHRLDSLEIFHRLGLSVFIF
                     DYRGYGKSEGKPTEEGVYSDAEGAWRHLAEDRKIPPGEIVIFGRSLGGAVAAQAAQTT
                     GIAQETLK"
BASE COUNT          203 a          299 c          357 g          180 t
ORIGIN      
        1 gggtggggaa gccctccctc gccgggaacc atcgccccgg gggcctcggt gaccgttacc
       61 gccttcgcca tgggcgacgc ggaaggcgtc gcggtgtctt ttgccggagg aggcacgtcc
      121 cttggttccc ttactgctct ttcagttcac agcacggaga aaagcgccga cggaacatac
      181 cggggagccg tgaccaacat cgggaaagga acgatcagcc ttaccgtctc cggacggtcc
      241 gcccgggcgg tcatcacggg gcactacacg gcagacggac agaacgtgaa aatttcggcc
      301 ggcggaagcg gaacctataa cccccggaag ggaaccgttt cgctgaaatg ggcgggaaca
      361 tccaacggct acatgctgga cgacggagag aggtatgacg taaacgaaag agtctccggc
      421 actctcagcg gatcattcca caaagggact ttttcaggaa aatggtgggg aggcagcgag
      481 ttcgtcatcc tcagcggaga gtggtcagcc cggtaaatcc ggggaggggg aagaaatgcg
      541 catgaggttt ctggtgtacc ttgccgcggg atatgtctgc ctggtcctgg cggcctctat
      601 ctttgccggg tcgctcatct tctttccccg gagggagctg gaggggaatc cgtcggaacg
      661 ggggctcccc ttcgaggacg tctccttccg ggcgggggac ggcacgtccc tccacgggtg
      721 gttccttccg ggaaaggacc gtggcactgt gctcttctgc cacgggaacg cggggaacat
      781 ctcccaccgg ctggattccc tggagatctt ccaccgcctg gggctgtcgg tcttcatctt
      841 cgactaccgg gggtacggga agagcgaagg gaagccaacc gaggagggcg tctacagcga
      901 tgcggaggga gcctggcggc atctcgctga ggacaggaag atccctcccg gcgagatcgt
      961 gatcttcggc cggtccctcg ggggtgccgt ggcggcgcag gcagcgcaaa ccacgggaat
     1021 cgcgcaggaa acgctgaag
//
LOCUS       DAOTHD010000954         1038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_175015_length_1038_cov_3.035605, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000954 DAOTHD010000000
VERSION     DAOTHD010000954.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175015_length_1038_cov_3.035605"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(296..595)
                     /locus_tag="P5219_12760"
     CDS             complement(296..595)
                     /locus_tag="P5219_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HRX27685.1"
                     /translation="MNFENSVTVTGSIHLPKGDMAGRNRNGVYLRFSLKHPSVGYDGV
                     ERTSFLPVRVFDAPLQEWLRGKGEGSPVRITGRLQTSSGSGEMYILAETLEEGAA"
     gene            complement(744..>1038)
                     /locus_tag="P5219_12765"
     CDS             complement(744..>1038)
                     /locus_tag="P5219_12765"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HRX27686.1"
                     /translation="LAQDEVDVIAAYSTDSRIKKLNLVLLEDDKSFFPDYSAAFVLPI
                     PLAEKYPGLVPVVEKISGAIDEQAMAAMNLAFDEGEDPEDIAAAFLRKKGFIQ"
BASE COUNT          210 a          295 c          304 g          229 t
ORIGIN      
        1 tgaggaattc gagcttcctg gtcttgttgc ccccggggaa gaaccccaga aaatcatctc
       61 ttttgatcca tatctcgggg ccgtcaagat aagcggacag cctgtcgagc ctttccaggg
      121 gcgtttctcc ttcggtgtac ctgcggcggg gaaaacgggc gagattcact tttatccagc
      181 tcctttaata aaaatcgaat ctgtcataaa cagtatacct tacggggaac aaaaaagaaa
      241 aacgtccctc ccgggcagcc gggagagacg ccgaacgatg ctcgtacagg aggagctagg
      301 ccgccccttc ctccagggtt tccgcgagga tgtacatctc gccgcttccg ctggaggtct
      361 gaagccggcc cgtgatccgc accggggagc cttcgccctt tccgcggagc cattcctgaa
      421 ggggggcatc aaaaacccgg acggggagaa aactcgtccg ctccacgccg tcatacccga
      481 cgctgggatg cttcagggaa aatctcaggt agacgccgtt ccggtttctt ccggccatgt
      541 cgcccttggg aaggtggatc gagccggtga ccgtgacaga attctcgaag ttcaacaaaa
      601 atccaactcc ttgctcgtta ttgatccccc gtcatggagg ggtgaaggaa cgataccact
      661 ctttgccctt tttcgcaaat ttttctttat tcctgaatcc gcaggcagcg gggagcgtca
      721 ccggtgctcg cttgggccgc cggctattga atgaacccct tcttccggag gaaggcggcg
      781 gcgatgtcct cgggatcctc tccctcgtcg aaggccaggt tcatggccgc catggcctgc
      841 tcgtcgatgg ctccgctgat cttctccacc acggggacga ggccggggta tttttccgcg
      901 aggggtatgg gcaggacgaa agcggcgctg tagtcgggga agaaactttt gtcgtcttcc
      961 agaaggacaa ggttcagctt ctttatccgg gagtcggtgg agtaggccgc gatgacgtcc
     1021 acttcgtcct gggcgagg
//
LOCUS       DAOTHD010000955         1038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_175022_length_1038_cov_2.927772, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000955 DAOTHD010000000
VERSION     DAOTHD010000955.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175022_length_1038_cov_2.927772"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="P5219_12770"
     CDS             complement(<1..121)
                     /locus_tag="P5219_12770"
                     /inference="COORDINATES: protein motif:HMM:NF012674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HRX27687.1"
                     /translation="MHRGLYAAASAMIVQETMHDVTANNLANVNTVGFKKVKTN"
     gene            complement(204..>1038)
                     /locus_tag="P5219_12775"
     CDS             complement(204..>1038)
                     /locus_tag="P5219_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRX27688.1"
                     /translation="PGPAAEAPLDPAHEVVQQGFNRFFRNRVMICVPSGATDVERRAV
                     LEAAVEVGAKEAYLIEEPMAAAIGANLNVEEPRGKMVVDIGGGTTDIAVISLGGIVVS
                     KSLRIGGDRFDEGIMRYLRKQYSLAIGEQTAENLKIMIGTCLQEEEETLMVLKGRDLV
                     QGLPRQIGVSSASVSMAIGEMVQSLIDGVRNVLELTPPELSADIIDRGIVLTGGGSLL
                     RGLPELITRHTGINCFTADDPMECVALGTGKALAEIDKLQALGRSGILVNKRKGRRRK
                     F"
BASE COUNT          163 a          326 c          319 g          230 t
ORIGIN      
        1 agttggtttt tacctttttg aagcccacgg tgttgacgtt tgccaggttg ttggccgtca
       61 cgtcgtgcat ggtttcctgc acgatcatgg ctgaagcggc cgcgtaaagg cctcggtgca
      121 tgaaattccc tcccggtggg ctctgcggag gcgggggccg ccgatccttc cggatcaatc
      181 cgtccccgcc cgaccgccgg ggatcagaat ttccgtcgtc tgccctttct tttgttgacg
      241 agaatgccgc tccttcccag ggcctggagc ttgtcgatct cggcaagggc cttgcccgtg
      301 ccgagggcga cgcattccat gggatcgtcc gccgtgaagc agttgatgcc cgtgtgcctg
      361 gtgatgagct cggggaggcc tctcagcagc gaaccgccgc ccgtgaggac gattcccctg
      421 tctattatat cggcggagag ttccggcggc gttagctcca gaacattccg aactccatcg
      481 atcaatgact ggaccatctc gccgatggcc atggagacgc tggcgctgga gaccccgatc
      541 tgcctgggaa ggccctggac aaggtcccgc cccttgagaa ccatcaatgt ctcctcttct
      601 tcctggaggc aggttccgat catgatcttg aggttttccg cggtctgttc gccgatggcg
      661 aggctgtact gcttccgaag gtagcgcatg atgccttcgt cgaaacggtc gcccccgatg
      721 cggagggact tggacaccac gatgcccccg agggatatga cggcgatgtc tgtggtgccg
      781 ccgccgatat ccaccaccat tttgccccgg ggctcttcca cgttgagatt tgcccctata
      841 gcggctgcca tgggctcttc gatgaggtag gcttccttgg ctcccacttc cacggcggcc
      901 tcgaggaccg cccggcgctc aacgtcggtg gctcctgacg ggacgcagat catgacccgg
      961 ttgcggaaga agcggttgaa gccctgctgc accacttcat gcgccgggtc cagcggggct
     1021 tcagccgctg gacccggc
//
LOCUS       DAOTHD010000956         1036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_175526_length_1036_cov_3.154944, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000956 DAOTHD010000000
VERSION     DAOTHD010000956.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175526_length_1036_cov_3.154944"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="P5219_12780"
     CDS             <1..296
                     /locus_tag="P5219_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRX27689.1"
                     /translation="SPFFVPSVIPANTYKGVTTDTHTPAVMAILVVDSKVPDKVVYDF
                     TKAFWTHIDDIQKVHAKAKLITPQTAMDGITVPVHPGAAKYYKEAKIKVPDIK"
     gene            complement(381..>1036)
                     /locus_tag="P5219_12785"
     CDS             complement(381..>1036)
                     /locus_tag="P5219_12785"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRX27690.1"
                     /translation="GVAKGGRKEENREALAKGFGNLDAHLENLSQFGVPVVVALNRFP
                     TDTEGELADVLAHVRGRGHEAALSEVWEKGGEGGMELAEKVLASMKKANGYHPLYELG
                     LPLKEKIEKIATHVYGAEGVDYAESASATLADLEKRGYGTLPVCMAKTQMSLSDDPGK
                     IGRPKGWRLLVREVRLSAGAGFVVPICGTIMTMPGLPKRPAAESIDVDEQGRISGLF"
BASE COUNT          197 a          386 c          245 g          208 t
ORIGIN      
        1 acagcccctt cttcgtcccc agcgtcatac ccgccaacac ttacaagggc gtgaccacgg
       61 acacccacac tcccgctgtc atggcaatcc tggtcgtgga ctcgaaggtt cccgacaagg
      121 tcgtctatga cttcacgaag gccttctgga cccatatcga cgatatccag aaagtccatg
      181 ccaaggcaaa gctgatcacc ccgcaaacgg ctatggacgg tatcacggtt ccggttcacc
      241 cgggcgccgc gaagtactac aaggaagcga agatcaaggt tcccgacatc aagtagaatc
      301 tcagcaatca cttttcatag aaaaggaatg gacgggcttg aaagcccgtc cattcctttt
      361 cttcttcagg tcattctttc ctagaagagc ccggagatgc gcccctgttc gtccacgtcg
      421 atggattcgg cagccggcct cttagggagt cccggcatcg tcatgatcgt cccgcagatc
      481 ggcaccacga atcccgctcc cgccgaaagc ctcacttcac gcaccaaaag cctccagccc
      541 ttcgggcgac cgatcttccc cgggtcgtcc gacagcgaca tctgcgtctt cgccatgcac
      601 accggcagcg ttccgtaacc tcgcttctcc aggtccgcca gggtggccga cgcgctctcc
      661 gcgtagtcca ctccctccgc cccgtaaacg tgcgtcgcga tcttctcgat cttctccttc
      721 agcggcaggc ccagctcgta cagcgggtga tagccgttcg ccttcttcat ggaagccagc
      781 accttctccg ccagctccat tcctccctcg ccgcccttct cccacacttc cgacagcgcc
      841 gcctcgtggc cccggcccct gacatgggca agaacgtctg ccagttcccc ctccgtgtcc
      901 gtggggaaac ggttcagcgc caccaccacc ggcacaccga actgcgacag gttctccagg
      961 tgcgcgtcca ggttaccgaa acccttcgcc agggcttccc ggttttcctc cttccggccg
     1021 cccttcgcca ctccac
//
LOCUS       DAOTHD010000957         1036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_175579_length_1036_cov_2.614679, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000957 DAOTHD010000000
VERSION     DAOTHD010000957.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175579_length_1036_cov_2.614679"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="P5219_12790"
     CDS             <1..588
                     /locus_tag="P5219_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRX27691.1"
                     /translation="PKEIQRVEERLVSRFEWGLVTDIQIPDLETRIAILQKKAILRGW
                     TIPEEVINFLATNIPRNIRELEGSLNRIVACAELNNEPMTIENAAVWLKDIIRHVSKG
                     PVTIEVIQQYVAEGYGLSVEDLTSSKRTAEVSLARQVAMYLSRSHTEASLQQIGYAFK
                     KKDHTTVIHACKKIGELLKSDPKVRGFVENVADKL"
     gene            830..>1036
                     /locus_tag="P5219_12795"
     CDS             830..>1036
                     /locus_tag="P5219_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRX27692.1"
                     /translation="MKLRIEKNSFVKTWQMAERNTSTTSTLSVLSGIRCLADGNTTIL
                     QATDLKTSVTCRAAGVTVEEAGEVV"
BASE COUNT          252 a          285 c          316 g          183 t
ORIGIN      
        1 cccaaggaga tccagcgggt cgaggaacgg ctggtgagcc ggttcgaatg ggggctggtt
       61 acggacatcc agatcccgga cctggagacg cgcatcgcca tcctgcagaa gaaggcgatc
      121 ctgcggggct ggacgatccc ggaggaagtc atcaatttcc tggccacgaa catccccagg
      181 aacatccgcg agctggaagg cagcctcaac cggatcgtcg cctgcgccga gctcaacaac
      241 gaacccatga cgatcgagaa cgcggcggtc tggctcaagg acatcatccg tcacgtttcg
      301 aagggaccgg tcaccatcga ggtgatccag cagtacgtgg ccgaaggcta cggcctgtcg
      361 gtggaggacc tgacgtcgag caagaggacg gcggaggttt ccctcgcgag gcaggtcgcc
      421 atgtacctca gccggtccca cacggaggcg agcctccagc agatcgggta cgccttcaag
      481 aaaaaggacc atacgacggt gatccatgcc tgcaagaaga tcggggaact gctgaaaagc
      541 gaccccaagg tccgggggtt tgtggaaaac gtggcggata agctgtgaaa tcccggtgct
      601 ggacagccgg tttatccaca ttttttcact gaatacactc tttccacaag aatcctgtgg
      661 atattgtgga caggatgtga gaagatccgg aaggtttttc cacagaggaa aatgccttcc
      721 cgcaacggtt taggggggtt ttccacgtat ccacggtccc taaagctcct cctactcttc
      781 atttatcttg aacagatgtg caaaagacgc aggaagggag tgtgacaacg tgaagttgcg
      841 gatcgagaag aactcctttg taaaaacctg gcagatggca gagcggaata cgagcacgac
      901 gagcactctc agcgtccttt cgggaatccg ctgcctcgca gacggcaaca ccacgatcct
      961 ccaggctacg gacctcaaga ccagcgtgac ctgcagggcg gcgggagtca ccgtggaaga
     1021 ggccggcgag gtcgtc
//
LOCUS       DAOTHD010000958         1033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_176394_length_1033_cov_2.502045, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000958 DAOTHD010000000
VERSION     DAOTHD010000958.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176394_length_1033_cov_2.502045"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1033
                     /locus_tag="P5219_12800"
     CDS             <1..>1033
                     /locus_tag="P5219_12800"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRX27693.1"
                     /translation="ALKKKIPEIAASLGTRFSVVPAPGAAPAEGSVEITTDSSGMATV
                     RQRWQKFLDQAPPLEIRLTEPREIYTEGRRAIFHSYLWIFLSGGGILVVVMVLMDSLV
                     LRRLDGLRSVSDRIVSGGGIGIRVPVSGDDEISVLSSSFNTLLDTLENLVAEIPDPLF
                     ICDPDGRILTANREAHRVLALGKPGELSGKDVSSILKPAENPKETGEDSRTGVIFSSA
                     DMFEATFERRDGTCLPVEIRRQEIRYGKRTLVLLLARDLTERKGFEQRLARKAYFDDL
                     TGLPNRSAFIEDLNRALKERNGFGEVLCAATLNLDRFKLVNEQVGNVNGDRILLIIGR
                     RLEETIGEDA"
BASE COUNT          208 a          320 c          318 g          187 t
ORIGIN      
        1 gccctgaaga aaaaaatacc cgagatcgcg gcgagcctgg gaactcgttt ttcagtcgtt
       61 ccggctcccg gggccgcccc cgcagaggga agtgtcgaga taacgaccga ttcttcaggc
      121 atggcgacgg tgcggcagcg ctggcagaaa ttcctggacc aggcgcctcc cctggaaata
      181 cggctcaccg aaccgaggga aatctacacc gagggacggc gggcgatttt tcactcctac
      241 ctctggattt tcctgagcgg gggcggcatc ctcgtggtgg tcatggtcct gatggattcc
      301 ctcgtgctgc gccggctgga cgggcttcgc tccgtttccg acaggatcgt cagcggcgga
      361 ggcatcggta tccgggtgcc cgtctcggga gacgacgaaa tctcggtcct gtcgtcctcc
      421 ttcaataccc tcctcgatac ccttgaaaac ctcgtggccg agattcccga ccccttgttc
      481 atctgcgatc ccgacggccg gattctcacg gcaaacaggg aggcccaccg cgtccttgct
      541 ctcgggaagc cgggggaact ctcaggcaag gacgtctctt cgatcctgaa gcctgcggag
      601 aacccgaaag aaacggggga ggattccagg accggcgtta tattcagcag cgccgacatg
      661 ttcgaggcaa ccttcgaacg acgggacggc acatgccttc cggtggagat caggaggcag
      721 gagatccggt acggaaaacg gactctcgtc ctcctcctcg cgcgggacct caccgagaga
      781 aagggctttg aacagcgcct cgccaggaag gcctatttcg acgaccttac cggtctcccg
      841 aaccgctccg ccttcatcga ggacctgaac agggccctga aggagcggaa cggtttcggt
      901 gaggttctct gcgccgccac gctcaacctc gaccggttca agctcgtcaa cgagcaggtg
      961 gggaacgtga acggcgacag gatcctgctt atcatcggcc gaaggctcga ggagaccata
     1021 ggggaggacg ccc
//
LOCUS       DAOTHD010000959         1033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_176424_length_1033_cov_2.317996, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000959 DAOTHD010000000
VERSION     DAOTHD010000959.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176424_length_1033_cov_2.317996"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    378..387
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <388..>1033
                     /locus_tag="P5219_12805"
     CDS             <388..>1033
                     /locus_tag="P5219_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRX27694.1"
                     /translation="RGEGVYLYDEEGKAYYDGCCGALLSSIGHGVKEIADAMYQQLLT
                     MEFAHPSRWNNKVSIEAAGEVASLTQEGLDYVWFVSGGSEAIESAMKIARQYFVERDG
                     KGSGKFEMIARWNSYHGSTVGTMGLAGSMGRRRVFAPLFRENPKICASYCYRCPLNLK
                     YPDCGVACARSLETEILRIGPESVAAFFAEPIVGSTVGALHPPKEYWPIVREICT"
BASE COUNT          217 a          299 c          326 g          181 t
ORIGIN      
        1 gctcaactgc ctcatcctct ccgtggcccg gagcctcggc gccggggtca cggaaaaact
       61 ggaattgctc gaacggcact ggagacagga gggaatctac attcccgaca ggacgaagcc
      121 cctgccgtag cgcccttttc cggcaggaat tcttattcgg gccccgggcc ccgcgaagga
      181 ggtatggaaa aaaccgcttc accgcttatc gtttcaggaa ctcatacaga aaggagctga
      241 tttttcggtg gatcttggac atctgattcc gagaagtttc aagggaaatc tgcggaccgc
      301 cgtacgcggc gagggagtgt tcatctacga cgaagagggc aaggcgtacg tgaccatcgg
      361 ggtggggtgc accggggnnn nnnnnnngcg aggcgaggga gtgtacctgt acgacgagga
      421 gggcaaggcc tactacgacg ggtgctgcgg ggccctgctc tcaagcatag ggcacggcgt
      481 gaaggaaata gccgacgcca tgtaccagca gcttctcacc atggaattcg cccacccctc
      541 ccggtggaac aacaaggttt caattgaagc ggccggggaa gtggcatctc tcacccagga
      601 agggctcgac tacgtctggt tcgtgagcgg cggaagcgag gccatcgaat cggccatgaa
      661 gatcgcccgc cagtatttcg tggagcggga cggcaaggga tccggcaagt tcgagatgat
      721 cgcccggtgg aattcctacc acggaagcac cgtgggaacc atgggcctcg cgggaagcat
      781 gggaagacgc cgcgtcttcg cccccctctt cagggagaac ccgaaaatct gcgcctccta
      841 ctgctaccgg tgccccctga acctgaaata tcccgactgc ggcgtggcct gcgcccgttc
      901 tctcgaaacg gagatcctca ggatcgggcc ggagtcggtg gcggccttct ttgccgaacc
      961 catcgtggga tccaccgtgg gagcccttca tccgccgaag gagtactggc ccatcgtcag
     1021 ggagatctgc acg
//
LOCUS       DAOTHD010000960         1031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_176963_length_1031_cov_2.345287, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000960 DAOTHD010000000
VERSION     DAOTHD010000960.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176963_length_1031_cov_2.345287"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..117)
                     /locus_tag="P5219_12810"
     CDS             complement(<1..117)
                     /locus_tag="P5219_12810"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRX27695.1"
                     /translation="MYIYNSLVRRKAHADNGWSQIDVQLKRRYDLIPNLVETV"
     gene            complement(231..>1031)
                     /locus_tag="P5219_12815"
     CDS             complement(231..>1031)
                     /locus_tag="P5219_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRX27696.1"
                     /translation="NGEFIEGARAALPFSDLAIQRGIAVFDSIRTYGGRPFAMDRHLE
                     RLAESARLSGINLPVPPEDMVSVIREGASRIPGDSIAKPFLTAGDVEENGTFPHPRFF
                     ILFSSLSPVPEEVYSKGLALTLLPQERELPRIKSINYMIPFCHREPGTFEPLYCPGGE
                     ITESATSSFFSVAGDNLITAPDHRVLRGITREIIVEVARENGLNVELRCLHLDELECV
                     SEAFITGSVKEVAPVVKVGDRVIGDGKPGHVTRKLIGLFRERIPLYLD"
BASE COUNT          214 a          299 c          320 g          198 t
ORIGIN      
        1 caccgtctcc accaggttgg ggatgaggtc gtaccgcctc ttgagctgca cgtcgatctg
       61 gctccagccg ttatcggcgt gggccttcct gcggacaagg gagttgtaga tgtacacggc
      121 ataaagtact gccgccgcaa gaagagcaag caggacataa agaatgttct ccaaggaaac
      181 ccctccttca ctgggcaata gctgacgtat gccagcatta taccttttct tcagtccagg
      241 tacagcggga tccgctccct gaacagcccg atcagcttcc gggtcacatg gcccggcttt
      301 ccgtcgccga tcaccctgtc cccgaccttc accaccggtg ccacttcctt cacgctgccg
      361 gtgatgaacg cttccgagac gcattccagc tcgtcgaggt ggagacaccg gagttccacg
      421 ttcaggccgt tctcccgggc gacctccacg atgatctccc tcgtgatgcc ccggaggacc
      481 ctgtggtccg gcgccgtgat gagattgtcc ccagcgacgg agaaaaaact gctcgtggcg
      541 gactcggtga tctcgccccc gggacagtag agaggttcga aagttcccgg ttcacgatgg
      601 cagaagggaa tcatgtagtt gatgctcttg atgcggggga gctccctctc ctgggggagg
      661 agcgtgaggg ccagcccctt tgaatacacc tcctccggaa cgggagaaag ggaagagaag
      721 aggataaaga atcggggatg ggggaaggtg ccgttctctt cgacatcccc cgcggtgaga
      781 aagggttttg cgatgctgtc gccggggatg cgggatgctc cctcacggat gacggaaacc
      841 atgtcctcag gaggcacggg gaggttgatg ccggagagcc gggccgattc tgcaagacgc
      901 tcgaggtggc ggtccatggc gaaaggcctg ccgccgtagg tccgaatgga atcgaagaca
      961 gcgatccctc tctggatggc gagatcggaa aaaggaagcg ccgcgcgggc cccctcgata
     1021 aattctccat t
//
LOCUS       DAOTHD010000961         1030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_177164_length_1030_cov_3.524103, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000961 DAOTHD010000000
VERSION     DAOTHD010000961.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177164_length_1030_cov_3.524103"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..519
                     /locus_tag="P5219_12820"
     CDS             145..519
                     /locus_tag="P5219_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRX27697.1"
                     /translation="MNHISQEEMNQSGLSRVSAEMPADETLYELADLFRVFGDTTRIK
                     ILCALSVSDLCVADIAALLSMNQSAISHQLRVLKQARLVRVARRGRSSIYGLDDDHVR
                     TIFEQGFRHVRHIAPGEEKTHA"
     gene            512..>1030
                     /locus_tag="P5219_12825"
     CDS             512..>1030
                     /locus_tag="P5219_12825"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HRX27698.1"
                     /translation="MPDGAGEIRLSLEGLNCAQCSATIEKKVAALPGVTSAAIDLVGG
                     RMRVSLSGKEHPEELLSSIRTVVSSVEPGVMVSEERSANAPSSLPKGDMIRLAAGAML
                     WLAALFLPLGETARLLLYVTAYLTAGIEVIRTVAGNLRRGTLFDEFFLMTAATGGAFA
                     IGEFSEAVAVMLF"
BASE COUNT          195 a          295 c          319 g          221 t
ORIGIN      
        1 agaggcggac gttcccggaa gggggcgtcc gcctttgcgc cgggaaaggc atgtttttgt
       61 tctcaagggg attgaaaaaa agacgtttcg gtggtatatg aatatatgat caggtgttca
      121 tatattcata taaggagaag agccatgaac catatctctc aggaagaaat gaaccagtcc
      181 ggtctttccc gggtctcggc ggagatgcct gccgacgaga cgctgtacga gcttgccgac
      241 ctgttccggg tcttcgggga caccacaagg atcaagatcc tctgtgccct ttcggtctcc
      301 gatctctgcg ttgcggacat cgcggcgctg ctttccatga accagtcggc catttcccac
      361 cagctcaggg tactcaagca ggcccgcctg gtgcgggtcg cccggagggg gcggtcctcc
      421 atttacggcc ttgacgacga ccacgtgagg accattttcg agcagggctt ccggcacgtc
      481 cgccacattg ccccggggga ggagaaaacc catgcctgac ggtgccggag aaatccgcct
      541 ctcccttgaa gggctgaact gcgcccagtg ctctgcgaca atcgaaaaaa aagtggctgc
      601 ccttcccgga gtcacatcgg cagccatcga tctcgtcgga ggcaggatgc gggtatcgct
      661 ctcgggaaag gagcatccgg aagagcttct ttccagcatc cggactgtcg tgtcgtccgt
      721 cgaaccgggg gtgatggttt cggaggaacg ttctgcgaat gccccatcga gcctgccgaa
      781 aggagatatg atccgccttg cggccggggc catgctctgg ctcgcggccc tcttcctgcc
      841 cctcggggaa acagcccggc tgcttttgta tgtgactgcc tacctgacgg caggaataga
      901 ggttatccgc accgtggcgg ggaacctccg ccgggggact cttttcgatg agttctttct
      961 catgacggcc gccaccggcg gcgcgttcgc catcggcgag ttttcggaag cggtggcggt
     1021 gatgctgttt
//
LOCUS       DAOTHD010000962         1030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_177181_length_1030_cov_2.957949, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000962 DAOTHD010000000
VERSION     DAOTHD010000962.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177181_length_1030_cov_2.957949"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..930)
                     /gene="istB"
                     /locus_tag="P5219_12830"
     CDS             complement(136..930)
                     /gene="istB"
                     /locus_tag="P5219_12830"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HRX27699.1"
                     /translation="MNAGRLLQLHENLKNLTLGNAALHLEERLRQAEERGASYEEFLL
                     ELTELELQIRSENREKRRLKEARFPLVKTLGSFTFEEAPQLDRRLIGELAAGDYISRH
                     RNILFLGKSGCGKTHLATALGIEACRKNLRVRFTTACNLVNELLESRGEKTLARTLGK
                     YTRYDLLIVDELGYVPFSKEGGELLFQVFAERHERGSVIVTTNLGFGSWTEVFGDPNM
                     TAALLDRMTHKAYIIECTWESYRLKETLREGRKRSQIQLKTAAAEE"
BASE COUNT          160 a          304 c          298 g          268 t
ORIGIN      
        1 atagagaagc ggagagttcc cggccacggc cggagattct ccgctttttt tgtgctctcc
       61 ggaccgtgtg gctgtcatct ggtttcgtca atgcaaatct gatcaccagg gacagccgaa
      121 ttttgaccac cccggtcatt cttccgcggc cgccgttttg agttgtattt ggcttctctt
      181 tcttccctcc ctcagtgttt ccttcagtct gtagctttcc catgtgcact cgatgatgta
      241 cgccttgtgg gtcatccggt cgagcagggc cgccgtcatg ttcggatctc cgaagacctc
      301 cgtccagctg ccgaatccga ggtttgtcgt cacgatgacc gatccccgct cgtgccgttc
      361 cgcgaagacc tggaacagca gttctcctcc ttccttcgaa aaggggacat atcccagttc
      421 gtccactatg agcaggtcgt accgggtgta cttgcccagg gtccgggcaa gggttttctc
      481 cccccggctt tccaggagtt cgttgacgag attgcaggcc gtggtgaacc ggacccgaag
      541 gttcttccgg caggcttcta ttcccagggc tgtggcaaga tgggttttcc cgcacccgct
      601 tttgcccagg aagagaatgt tccggtgccg ggagatgtag tctcctgcgg ccagttcacc
      661 gatgagtctt ctgtcaagct gcggcgcctc ttcgaaggtg aagctcccca gggttttgac
      721 cagggggaaa cgggcttcct tcagccgcct cttttccctg ttttcggagc ggatctggag
      781 ctccagttcc gtgagttcca ggaggaattc ttcgtagctc gctccccgtt cttctgcctg
      841 gcggagccgt tcctcaaggt gcagggctgc gttgccgagg gtcaggttct tcaggttttc
      901 gtgaagctgc agaaggcgcc cggcattcat tgtccggcct ccaggacgga atagacggat
      961 acgtctgccg cgggaaggac ggtccaccgt tccgactcga ggggacgggt cgtttcttcc
     1021 tgtctttcgg
//
LOCUS       DAOTHD010000963         1030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_177222_length_1030_cov_2.404103, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000963 DAOTHD010000000
VERSION     DAOTHD010000963.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177222_length_1030_cov_2.404103"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..>1030
                     /locus_tag="P5219_12835"
     CDS             61..>1030
                     /locus_tag="P5219_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRX27700.1"
                     /translation="MERKKVLIIGGVACGAKTAARLARLAPEFDITVLERGEHLSFAG
                     CGFPYFVGDVVKEYKDLVCTPLGIIRDANFFRTVKNITVHTGCLATRIDREKKCVTAS
                     DASTGEEKTFPYDNLVLATGASPIRPPIPGSDLNRVFTLWTMPDALAMKSALSSGNIK
                     TAVIIGAGLIGMEVVEALTERGVRVSVVDALPTPLPALVGEDFGLRVAKAVKAKGVDF
                     FGGEKVTEITGEGGVVSGVRTDKRSLPADMVLLAVGIRPNTSLARDAGLELAPNGTIV
                     VDELMRTSDPSIYAGGDCVQTRNLVTKKPVWQPMGSTANRQGRVIAD"
BASE COUNT          216 a          322 c          321 g          171 t
ORIGIN      
        1 cgccgtggag aataccatat aggggatagt ggtattattc caaaaggagg ggtagagttc
       61 atggaaagaa aaaaggtgct catcatcggg ggcgtggcgt gcggcgccaa gacggccgcc
      121 aggcttgcca ggcttgctcc ggagttcgac atcacggtgc ttgagcgggg ggaacacctc
      181 agtttcgccg gatgcggctt tccctatttc gtgggggacg tggtgaagga gtacaaggac
      241 ctggtctgca ctcccctcgg catcatccgg gacgcgaact tcttccggac ggtgaagaac
      301 atcacggtcc acaccggctg cctcgccacc cggatcgaca gggagaaaaa gtgcgtcacc
      361 gcctccgatg catccaccgg cgaagaaaag acctttccct acgacaacct cgtgctcgcc
      421 acgggcgcgt cccccatccg cccccccata ccgggcagcg atctcaacag ggtctttacc
      481 ctctggacca tgcccgacgc cctggccatg aaatcggccc tctcgtcggg gaacatcaaa
      541 acagcggtca tcatcggagc cggcctcatc ggcatggaag tcgtggaggc ccttacggaa
      601 aggggtgtca gggtttccgt ggttgacgcc cttcccacgc cccttccggc cctcgtgggc
      661 gaagatttcg gcctccgggt cgcaaaagcg gtcaaggcaa aaggcgtcga tttcttcgga
      721 ggcgagaagg tcaccgaaat caccggcgag ggaggggtcg tcagcggagt caggacggac
      781 aagcgctccc tgcctgcgga catggtgctc ctggcagtgg gcatccggcc caacaccagc
      841 ctcgcccggg acgccggcct ggagctcgct cccaacggaa ccatcgtggt ggacgaactg
      901 atgaggacga gtgacccttc catctacgcc gggggcgact gcgtccagac acggaacctt
      961 gtcacgaaaa agcccgtatg gcagcccatg ggctccacgg caaaccgcca gggcagggtc
     1021 atcgcagaca
//
LOCUS       DAOTHD010000964         1029 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_177498_length_1029_cov_2.349076, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000964 DAOTHD010000000
VERSION     DAOTHD010000964.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177498_length_1029_cov_2.349076"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..>1029
                     /locus_tag="P5219_12840"
     CDS             163..>1029
                     /locus_tag="P5219_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27701.1"
                     /translation="MKTKKILNCHGSLVLVLLGVFLLASAGGAAAQEQEEKALSFQEI
                     AAEVEKRLPDAEEKLLAGAAAVEQLLKSSKTEAGEMTGKWNEILEQSFASPVMKELAQ
                     LRPLLKVTIDRGLHEAMQASRERMTKGDAPVASTSGKSGITLPIARDIHKDAEELLGF
                     AQEIQSVGEAVAWTLKVNRHIESLEFDIGNAPARVGAYVDDMKLMSASLADIRRKANQ
                     AMEDYSKTRGSLAELKGEMSRYLTLTMAMKYKTQNAAVSLVNTAKYLEPETGYVIPAT
                     ELKRMEVLAEYWK"
BASE COUNT          242 a          274 c          343 g          170 t
ORIGIN      
        1 ggagaagttg tggaacaggc cctcgtgacc acccgggcgt gggatgagag gggacgccag
       61 tacgggtctc ccaagcccca tttccttatt cggaaaaaca aggggcgctg gtacatcaac
      121 aacttcgagc agcccttcta gaaggggacg acggaggacg tcatgaaaac gaaaaagatc
      181 ctgaactgtc acggaagtct tgtccttgtg ctcctaggag tctttcttct tgcatcggcg
      241 ggcggggcgg cggcccagga gcaggaggaa aaggcgctga gtttccagga aattgccgcc
      301 gaggtggaaa agaggctgcc ggacgcggag gagaagctcc ttgcgggagc ggcggcggtg
      361 gagcagctcc tgaagtcttc aaagaccgag gcaggggaga tgacgggaaa gtggaacgag
      421 atccttgagc agtccttcgc ctctcccgtc atgaaggaac tggcccagct ccgccccctg
      481 ctgaaggtga ccatcgacag gggactgcat gaagcgatgc aggcgtcacg ggaaagaatg
      541 accaaggggg atgcgcctgt tgcttcgact tcgggaaaat ccgggatcac tctccctatt
      601 gccagggaca tccacaagga cgccgaggaa ctgctcggct tcgcccagga gatccagtcc
      661 gtgggagaag cggttgcctg gacgctgaag gtgaaccggc acatcgagtc cctggagttc
      721 gacatcggca acgccccggc ccgggtcggc gcttacgttg acgacatgaa gctcatgagt
      781 gcatccctgg cggatatccg ccggaaggcg aaccaggcca tggaggacta ttcgaaaacc
      841 cgggggtctc ttgcggagct caagggagag atgtcccggt atctgacgct gacgatggcc
      901 atgaaataca agacccagaa tgccgccgtc agccttgtga acacggcgaa gtacctcgag
      961 ccggagacag ggtacgtcat cccggcgacg gaactcaagc ggatggaagt cctggcggag
     1021 tactggaag
//
LOCUS       DAOTHD010000965         1028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_177707_length_1028_cov_3.134635, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000965 DAOTHD010000000
VERSION     DAOTHD010000965.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177707_length_1028_cov_3.134635"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1028)
                     /locus_tag="P5219_12845"
     CDS             complement(<1..>1028)
                     /locus_tag="P5219_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRX27702.1"
                     /translation="LFPPVSCIITCYSEGKDIQKTIMSLAAQLYPGKIEIIPVLDGAS
                     ANKPTYDAAYEMEKEVNSLPGRVLKCLPKWKRGGRVSSLNSGFALSTGDVIMAMDGDT
                     SFDNDMVENAVRHFSNPDVMCVSGCLRVRNWRDSPAAALQAVEYLVSILASKTGLSEF
                     NAVNNISGAFGIFRRDVLTLIGGWDAGTAEDLDLTLRVKNYFGRHRNMRIVFDPEVMG
                     HTDAPDTFRGFFRQRLRWDGDLFYLYFLKHFRSFSPRLMGWPNLIVSTVGGLLFQIVT
                     PLLLFLYTVFLLATVPMTVVGVIMGLVYLFYFSVETFFFLVMLFFLSERPREDAMLIP
                     LLPLMPVF"
BASE COUNT          242 a          264 c          333 g          189 t
ORIGIN      
        1 gaatacgggc atgagaggaa ggagaggaat gagcatggcg tcttccctcg ggcgctcgga
       61 gaggaagaag agcatcacga ggaagaaaaa ggtttccacc gagaagtaaa aaagatagac
      121 gagacccatg atgactccga cgacggtcat ggggacggtt gccagcagga atacagtgta
      181 gaggaagaga agcagggggg tgacgatctg gaaaagaaga ccgcccaccg tggagacgat
      241 aaggttgggc catcccatga gcctcggcga gaaagagcgg aaatgtttga ggaagtagag
      301 atagaaaaga tccccgtccc accggagcct ttgcctgaag aaaccgcgga aggtgtccgg
      361 ggcgtcagta tggcccatca cctcggggtc gaagacgatg cgcatgttcc ggtgcctgcc
      421 gaaatagttc ttcaccctga gggtgagatc caggtcttcg gcggtcccgg catcccatcc
      481 gccgatgagg gtgagcacat ctctcctgaa gatgccgaaa gctccggaaa tgttgttgac
      541 cgcgttgaat tcgctgagcc ccgtcttgct cgcgaggatg gacacgaggt actcgacggc
      601 ctggagggca gcggcggggg aatccctcca gttgcggacc cggaggcacc cggagacgca
      661 catcacatcg ggattgctga agtggcggac ggcgttttcc accatatcgt tgtcgaagga
      721 ggtatccccg tccatggcca tgataacgtc tcccgtggag agggcaaaac cggagttgag
      781 ggaggaaacc cgcccccctc tcttccactt gggaaggcat ttcagcaccc ttcccgggag
      841 ggaattcacc tctttctcca tctcataggc cgcgtcgtag gtgggcttgt tggccgatgc
      901 accgtccagc acggggatga tctctatctt tccgggatac agctgcgcgg ccaggctcat
      961 gatggtcttc tggatatcct ttccctccga gtagcaggtg atgatgcagg atacgggcgg
     1021 aaagaggg
//
LOCUS       DAOTHD010000966         1028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_177767_length_1028_cov_2.469681, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000966 DAOTHD010000000
VERSION     DAOTHD010000966.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177767_length_1028_cov_2.469681"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..602)
                     /locus_tag="P5219_12850"
     CDS             complement(120..602)
                     /locus_tag="P5219_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27703.1"
                     /translation="MKRPYGVIAAIVLFFSAVVILLVSRWLTAEVNAFRDRGVTTMVR
                     ILGFREVSSGDTGTRTIALAEIVTDGEDDAVRDIAASHILMDEITEVGSLRHGQKLEV
                     IVLPDGEFSMTPPGYTHWFVTRRSLDRHLRNSLSQWGWNSAVVLTVLAMLLWIRSGRK
                     "
BASE COUNT          246 a          287 c          279 g          216 t
ORIGIN      
        1 actggtggac ctggacgaat aaaggaggga aagaaaagaa gacctactgc ggggccgacg
       61 cgtttgccgg gaaagtgtct gaaggctgcc ccgccggagc ctggttcccg gtaagccgtc
      121 tactttcggc cggaccggat ccagagaagc atcgcgagca ccgtcagcac aaccgctgaa
      181 ttccatcccc actgcgaaag ggaattcctc agatggcggt cgagggagcg gcgggtgacg
      241 aaccagtgag tgtagccggg aggcgtcatc gaaaattccc cgtcgggaag gacgatcact
      301 tccagtttct gcccgtgccg gagcgaaccc acttccgtta tctcgtccat caaaatgtgg
      361 gacgccgcaa tatcccttac ggcatcatcc tcaccatcgg tcacgatctc ggcaagagct
      421 atcgttcttg tgcccgtgtc gccgctggaa acctcccgga atcccagaat ccgcaccatg
      481 gtcgtcacgc cccggtcccg gaaagcatta acctctgcgg tgagccaccg ggagacgaga
      541 aggatgacga cagccgaaaa aaagaggact atcgcggcga ttacaccata gggtcttttc
      601 atttttcccc ctgtttttcc atgaaaattc cacgaagaat attctatcgc ctgaagcggc
      661 ggaagaacag cggcgttttc cccccaaaaa acaggcccct cctgttccgc cgccgcactt
      721 cctgtacatg ggacatcccc cggcgggaca cgtcggcagc cggcccgcct gaaggcttat
      781 ttatacgtgc atattttgaa catcataccg aagggacttt tttcattgac aagtttgctg
      841 agttatgaat ataatcaggc tgcacttatt tggtaaccat ggtataccag aaactcgccc
      901 gggaggtttc cttttcccga atggattttc ccatgacagg agagtgagca ctatgaccta
      961 caggatgcgt ccgccgaggg atgtggtttc cggaaaagga agcctggaac agatagggga
     1021 gattgttt
//
LOCUS       DAOTHD010000967         1024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_178905_length_1024_cov_2.106295, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000967 DAOTHD010000000
VERSION     DAOTHD010000967.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178905_length_1024_cov_2.106295"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="P5219_12855"
     CDS             <1..496
                     /locus_tag="P5219_12855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX27704.1"
                     /translation="AREIREYCDELSPSWIRLGLASAMQENADRLSRAYEGLVIDVDV
                     DEDLDVLSEESSLALVRIFQEAVSNSARHGGASSVRAALKKEGDAVHFTIEDDGGGFD
                     AGPASGTDFELLRTTGHRGLANMNERVRLLGGTMKLESSPGQGCRIDIVFPLAREEST
                     AAPA"
     gene            529..669
                     /locus_tag="P5219_12860"
     CDS             529..669
                     /locus_tag="P5219_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27705.1"
                     /translation="MIEMLSAVKADITTLQVDAIVNAANNSLLGGGEWTGQYTVPRGR
                     IC"
     gene            627..>1024
                     /locus_tag="P5219_12865"
     CDS             627..>1024
                     /locus_tag="P5219_12865"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HRX27706.1"
                     /translation="MDGAIHRAAGPDLLNECRKLGGCATGDARITKGWRLPAKYVIHT
                     VGPVWHGGGRGEAELLASCYRRSLELASGAGARSIAFPSISTGVYGYPTEQAARIAVS
                     TVREELEKYPGIEEVVFCCFSEGDRAVYDKL"
BASE COUNT          209 a          306 c          334 g          175 t
ORIGIN      
        1 cgccagggaa atccgggaat attgcgacga actgtccccc tcgtggatcc ggctcggcct
       61 ggccagcgcc atgcaggaga acgccgacag gctttcccgc gcctacgagg gcctggtgat
      121 cgacgtggat gtggacgagg atcttgacgt tctttccgaa gaaagcagcc tggccctggt
      181 ccgcattttc caggaagctg tatcgaactc ggcgcggcac ggaggggcca gttccgtcag
      241 ggcggcgctg aaaaaagaag gcgatgccgt ccatttcacc attgaagacg acggcggcgg
      301 attcgacgca ggacctgcgt ccgggacgga tttcgaactg ctccgcacca cggggcatag
      361 ggggctcgcc aacatgaacg aacgggtgcg gctcctcggc ggcaccatga aacttgaatc
      421 ctctcccggg cagggctgcc ggatcgacat cgtctttccc ttggcgcggg aggaaagtac
      481 cgccgcccct gcatgagatc gacggatacc gtacattctt ttcggaaggt gattgaaatg
      541 ctgtcagctg taaaagcgga tataacgacc cttcaggtgg atgccatcgt caacgcagcc
      601 aacaattccc tgctcggcgg cggggagtgg acggggcaat acaccgtgcc gcggggccgg
      661 atctgctgaa tgaatgcaga aagctcggcg gctgcgccac cggcgacgcc agaatcacga
      721 aaggctggag gctcccggcg aagtacgtca tccacaccgt gggccccgtg tggcacggag
      781 gcggccgggg agaggcggag ctcctggcat cgtgctacag gcgctccctg gagctggcat
      841 caggggcagg ggcgaggtcc atcgccttcc cctccatcag caccggcgtc tacggctacc
      901 cgaccgaaca ggccgcccgg atcgccgtgt ccacagtgcg ggaggaactg gaaaaatatc
      961 ccggcattga agaagtcgtt ttctgctgct tctccgaagg ggaccgtgcg gtctacgata
     1021 aact
//
LOCUS       DAOTHD010000968         1022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_179514_length_1022_cov_1.831437, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000968 DAOTHD010000000
VERSION     DAOTHD010000968.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179514_length_1022_cov_1.831437"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1022)
                     /locus_tag="P5219_12870"
     CDS             complement(<1..>1022)
                     /locus_tag="P5219_12870"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRX27707.1"
                     /translation="VYANAGLPGDGDCISPERFAALGQKLAGAGAAVVGGCCGTTPEH
                     VAALKGALGDLLPVRSTPAPGTPLASRSRLVLAGKGHPFLVVGERINVSRKSPLRDEI
                     ARGEWSTLREEARNQTAAGAMVLDVNVGLPTIDQEAAMKAAVAAAEQSSDLPLSIDSD
                     SRSVLESGLCAVTGIPLINSFTARDDSLYPGLALAKLHGAAAAVLPIDAKGLPEKAEE
                     RIAVIRNILSAADSLGFPRNALIVDGLTLAAGADMNATAATLAVLAFLRDEGITSMLG
                     VSNISHGMPARALLNRTFLAMAVSSGLDSAIMNPLDPWMMETLAASELLSGRDPRGSR
                     FLTGAP"
BASE COUNT          172 a          321 c          356 g          173 t
ORIGIN      
        1 cggggctccg gttaaaaagc ggcttcccct ggggtccctc ccggaaagca gctcggaagc
       61 ggcgagggtc tccatcatcc acggatccag gggattcatg atcgccgaat cgaggccgga
      121 cgagactgcc atggcgagga aggtcctgtt cagcagggcc cgggccggca tgccgtggga
      181 gatgttgctc actccgagca tggaggttat cccctcgtcc cgaagaaagg cgagcaccgc
      241 cagcgtagcg gcggtggcgt tcatgtcggc tcctgccgcg agggtcaggc cgtccacgat
      301 gagggcgttc cggggaaaac cgagggagtc cgccgctgaa aggatgttcc tgatgacggc
      361 gatccgctct tccgccttct caggaaggcc ctttgcgtca atggggagga ccgccgctgc
      421 cgccccgtga agcttcgcca gggcaagccc cgggtacagg gaatcatccc tggccgtgaa
      481 agaattgatc agggggatgc cagtgacggc gcacaggccg gattcgagca ccgaacggga
      541 gtcgctgtct atggagagag gaaggtccga cgactgttcc gcggcggcga cggcggcctt
      601 catggccgct tcctggtcga tggttggaag acccacgttg acgtcgagca ccatcgctcc
      661 ggctgcggtc tggttccgtg cttcctcccg gagtgtgctc cattcccccc tggcgatttc
      721 gtccctgagg ggagatttcc gtgagacgtt aatccgctct ccgaccacga ggaagggatg
      781 tcccttcccg gcgaggacga gcctgctcct gctcgccagg ggagtacccg gggcgggggt
      841 gctccggacg ggaagaaggt ctccgagggc gcccttcagg gcggccacat gctccggagt
      901 ggtcccgcag catcctccga ccacagcggc cccggccccg gccagtttct gccccagagc
      961 ggcgaagcgc tccggggata tgcagtcgcc gtctccggga aggccggcgt tggcgtagac
     1021 ga
//
LOCUS       DAOTHD010000969         1021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_179705_length_1021_cov_2.285714, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000969 DAOTHD010000000
VERSION     DAOTHD010000969.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179705_length_1021_cov_2.285714"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="P5219_12875"
     CDS             complement(<1..728)
                     /locus_tag="P5219_12875"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HRX27708.1"
                     /translation="MKEKSRRMTLMMKLGGGTALLVVLVTVLCVGAAVMIFTGFLEND
                     ARQKAELAVDGLENILTSKKDVTRAVTLFLARYGSIAGDVVSGDTAALIEKLTPMWKE
                     TDLDFVTVLDARGNVLARLHEPDKKGDSLANQANVRGALAGQVTTVVEPGTQIPLSAR
                     TGVPVKDASGKVVGVVSAGLSFSREAFVDQVKKLFNAEVTIFAGDTRLMTTIIQDGKR
                     VVGTKLDPAVAAKVLGGQRFEGRA"
BASE COUNT          225 a          313 c          257 g          226 t
ORIGIN      
        1 tccgcccgcc cctcgaaccg ctgccctcca agtactttcg cggcgacggc gggatcgagc
       61 ttcgtcccca cgacccgctt tccgtcctgg atgatggtgg tcatgagcct ggtgtcaccg
      121 gcgaagatgg tcacctcggc gttgaaaagc ttcttcacct ggtcgacgaa cgcctcccgg
      181 gagaaggaga gcccggcgga cacaacgccg acgaccttcc ctgaagcgtc cttcacgggc
      241 accccggtac gggccgaaag aggaatctgg gtaccgggct cgacgacggt ggtcacctgc
      301 cctgccaggg ctccccggac gttcgcctgg ttcgcaagag agtccccctt cttgtccggc
      361 tcatggaggc gggcaaggac atttcccctg gcgtcaagga cagtcacgaa gtcgaggtcg
      421 gtctccttcc acatgggggt gagcttttcg atgagcgccg ccgtgtcccc gctgaccacg
      481 tccccggcga tgctgccgta ccgggccagg aagagggtca ctgcccgtgt cacgtccttc
      541 ttggaggtca gaatgttttc cagcccgtca acggccagct ccgccttctg cctggcatcg
      601 ttctcgagaa acccggtaaa aatcatcacg gcggctccga cacaaagcac ggtaacgagg
      661 actaccagca gggcggtgcc tccgccgagc ttcatcatga gggtcattct gcggctcttt
      721 tccttcatcg acaaacacct ctttcaaaaa ataagtttaa atattccatt acttttcttt
      781 tcgacttttt tttcgggtat catgagcaag aacggtcaga atataactta tcataggaca
      841 aaacggcatt accaataata atcaatatga acaatatgcc ctttaccgcc ggacagaaaa
      901 atttcggctc ctctttcatt ccttctcttg agaacaaaac cactggtcac acatcaacaa
      961 acggaatggc acaaaattgt agtttcgtgc taccatatgg ccgtccaggt tcatcatcag
     1021 a
//
LOCUS       DAOTHD010000970         1020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_179926_length_1020_cov_2.800000, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000970 DAOTHD010000000
VERSION     DAOTHD010000970.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179926_length_1020_cov_2.800000"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="P5219_12880"
     CDS             <1..863
                     /locus_tag="P5219_12880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX27709.1"
                     /translation="GGWGDWRDNMPQIHVLLAEDNKTNQEVTCALLKHAGIETTVAGD
                     GREAVEIFRKGPDTFDLVFMDVQMPVMDGLQATRAIRALPGGKAVPIVAMTANAFEED
                     GRMCIEAGMDAHLSKPLEPDRLLRVLMERIPRLRSGQAAAERESAAVPSSGQCPDALP
                     PGLDALPGLDVRAGLRSVRGDVPFFLGLLSQFADQHGRDGREIAAALEKGTAEDARHR
                     AHGLRGVSLTLGLEEIHSLSAEIEKLLRKECPAEEILPLAKTLEEELDRFTARVRDFL
                     PKGSPGGPEG"
BASE COUNT          196 a          313 c          348 g          163 t
ORIGIN      
        1 gcggaggctg gggagactgg agggacaaca tgccgcagat ccatgttctc ctggcggaag
       61 ataacaaaac caaccaggaa gtaacctgcg ccctcctcaa acacgcggga atagagacta
      121 ccgtggcggg ggacgggcgg gaggccgtgg agatcttcag gaaagggccc gacaccttcg
      181 acctggtctt catggacgtg cagatgcctg tgatggacgg cctgcaggcc acccgcgcca
      241 tcagggccct tcccggcgga aaggcggtcc ccatagtggc catgaccgcc aacgccttcg
      301 aggaagacgg gagaatgtgc atcgaggcgg gcatggatgc tcacctgtcc aagcctctgg
      361 aacccgaccg gcttctgcgg gtgctgatgg aacggattcc ccgccttcgc tccgggcagg
      421 ccgctgccga aagggaatcc gccgcagtgc cgtcatcggg tcagtgccct gacgcccttc
      481 ccccgggttt ggatgctctg cccggcctcg atgtgcgggc gggcctccgg tcggtgagag
      541 gagacgtgcc cttctttctc ggccttttga gccagttcgc cgaccagcac gggcgggacg
      601 gcagggagat agcggccgcc ctggaaaagg gaacggctga agacgcgcgg catcgtgccc
      661 acgggctcag gggagtgtca ctcaccctcg gcctggagga aatccactct ctctcggcgg
      721 aaatcgagaa gctcctgcgg aaagaatgcc cggctgaaga aattctcccc ctggcgaaaa
      781 cccttgaaga ggagctggac cgcttcactg cccgtgtgag ggactttctc cccaaggggt
      841 cccccggcgg tcctgaggga tagtcactcc gtcctcggaa gggaccaccg gccgcaggtt
      901 ctttccggtg agaaaaggtc cggaagggag gatatgcggc cgtcggggca cttgaaatca
      961 ccctctccgg aaaggggggc gtcgcccatg aggatggcgg agacgccggc ccggtgggcc
//
LOCUS       DAOTHD010000971         1020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_179927_length_1020_cov_2.792746, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000971 DAOTHD010000000
VERSION     DAOTHD010000971.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179927_length_1020_cov_2.792746"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="P5219_12885"
     CDS             complement(<1..427)
                     /locus_tag="P5219_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRX27710.1"
                     /translation="MKHDRFCLLLLVLLLLVSPAGGEEKLSLMLDWFPNVNHVPLFVA
                     RQGGFFSKHGLSVELQSPSDSADPLQLAAAGHVDIALSYQPQAIIAASAGIPLKAVGR
                     LVGSPLSTLLFLDGKGITSPADLEGKTIGYTVPGMMDHLL"
     gene            complement(472..>1020)
                     /locus_tag="P5219_12890"
     CDS             complement(472..>1020)
                     /locus_tag="P5219_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRX27711.1"
                     /translation="SLAVGVPLSIAMFFSPALEKAISPLLIASQAIPVFAAAPLLVVW
                     FGYGMGSKVAMAAVIIFFPVTVTLLQGFKSCDPDMKTLFSVMGAGFLTTLRHLYWPWA
                     LPYFFAGLKVAVSVAAIGAVIGEWVGSTDGLGFLMMQANARLRVDLVFASIVVLSAVS
                     LSLWGTVCIFEKNTVRWTERKR"
BASE COUNT          244 a          284 c          321 g          171 t
ORIGIN      
        1 ggagaaggtg atccatcatg ccgggaacgg tataaccgat ggttttcccc tcaaggtcgg
       61 cgggagacgt tattcccttc ccgtcgagaa agaggagagt cgagaggggg ctccccacga
      121 ggcgccccac ggctttcaga ggaattcccg ctgacgccgc gatgatcgcc tggggctggt
      181 aggaaagagc gatgtccaca tgtccggcgg cggcgagctg caggggatcg gcgctgtcgg
      241 agggactctg cagctccacc gaaagtccgt gtttcgagaa gaatccaccc tggcgggcga
      301 cgaagagggg aacatggttc acgttgggga accagtcgag catcaggctg agcttttctt
      361 ctcccccggc gggagaaaca agaagcagca ggacaaggag cagcaggcag aagcggtcgt
      421 gtttcatagc atttcctccg atcatagatt ttgtaacggt gcggtgcccc gtcaccgttt
      481 cctttccgtc caccggacgg tgtttttctc gaagatgcag acggttcccc acagtgaaag
      541 gctgaccgcc gaaaggacga cgatagaagc aaaaaccagg tcaaccctca gcctggcgtt
      601 cgcctgcatc ataaggaatc cgaggccgtc cgtgcttccc acccattcgc cgatgaccgc
      661 tccgattgcc gccaccgaga cggcaacctt gaggccggcg aaaaaatagg gaagggccca
      721 gggccagtag agatggcgta gggttgtcag gaagcctgcc cccatgacgg aaaaaagggt
      781 tttcatgtcg ggatcgcagc tcttgaatcc ctggagcagg gtcaccgtta ccggaaaaaa
      841 gatgatcaca gccgccatgg ccaccttgct ccccatgccg taaccgaacc acaccaccag
      901 aaggggagcg gcggcgaaga ccgggatggc ctgcgaggcg atcagcaggg gagaaatcgc
      961 tttttcaaga gctggagaga aaaacatggc aatggacagg ggcacgccca cagccaggga
//
LOCUS       DAOTHD010000972         1019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_180161_length_1019_cov_3.297718, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000972 DAOTHD010000000
VERSION     DAOTHD010000972.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180161_length_1019_cov_3.297718"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..549)
                     /locus_tag="P5219_12895"
     CDS             complement(76..549)
                     /locus_tag="P5219_12895"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRX27712.1"
                     /translation="MKKILTLMEKTFAFLSMAGIGLMLIIIFLQVISRYFFGYTASYS
                     EELSRYLFVWVTFLSLPVVSRQGGHMVVGLLTERFTGERLRRLKIAGCICSMAFLAIM
                     VWQGIRMVQLAVWQTSPAMEIPMSYMYISIPLGCGGMLLLVLEELLDLVLRREAA"
     gene            complement(617..>1019)
                     /gene="dctP"
                     /locus_tag="P5219_12900"
     CDS             complement(617..>1019)
                     /gene="dctP"
                     /locus_tag="P5219_12900"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRX27713.1"
                     /translation="ELFTAMQQGVVDGQENPAAHIYTSRFFEVQKYISLTAHTYSAEP
                     LLVAMGVWSKLPEAHQKSIQEAAFEARDWQRDLCRGLENEYWTKIRESGKSEINDDVD
                     KAAFMEATKPVWAMFEEKLGKQNIEAIVNVK"
BASE COUNT          214 a          273 c          317 g          215 t
ORIGIN      
        1 gggcggctcc gatgggcgcc ccggtcagca gtatggtgag gaagaagaag agaaggagcc
       61 agagagtgat atccgtcatg ccgcttccct cctcaggacg agatcgagaa gttcttcaag
      121 gacaaggagg agcatgcccc cgcatcccag ggggatggaa atgtacatgt aggacatggg
      181 gatttccatg gccggcgacg tctgccagac tgcgagctgt accatgcgga ttccctgcca
      241 gaccatgatg gcgaggaacg ccatgctgca gatgcagccc gcgattttca gcctgcggag
      301 tctttcgccg gtgaaccgct ccgtgagcag ccccacaacc atgtgtcctc cctgccggga
      361 gaccacgggc agggagagga aggtcaccca gacgaagaga taccgtgaga gttcctccga
      421 atagcttgcg gtgtagccga agaaataccg ggaaatcacc tgcaggaata tgatgatgag
      481 cataaggccg atgcctgcca tggaaaggaa ggcaaaggtt ttttccatga gagtgaggat
      541 ttttttcatt cagccgcctc cgttctggcg aaaaaaagaa aaagggagcg gtatggggcc
      601 gctccctttc ggcaggctat ttcacgttga cgatggcttc gatgttctgt ttgccgagct
      661 tttcctcgaa catggcccac acgggcttgg tcgcttccat gaaggcggcc ttgtccacat
      721 cgtcgttgat ctcgctcttt ccgctctcgc ggattttcgt ccagtattcg ttctcaagcc
      781 cccggcacag gtcgcgctgc cagtcgcggg cctcgaatgc ggcttcctgg attgacttct
      841 gatgcgcctc gggaagcttg ctccagacgc ccatggctac gaggaggggt tccgcggaat
      901 aggtgtgggc ggtgagagag atgtacttct gtacctcgaa gaaccgggag gtgtagatgt
      961 gggccgccgg gttctcctgg ccgtcaacaa ctccctgctg catggcggtg aacagttcg
//
LOCUS       DAOTHD010000973         1017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_180725_length_1017_cov_3.007277, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000973 DAOTHD010000000
VERSION     DAOTHD010000973.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180725_length_1017_cov_3.007277"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="P5219_12905"
     CDS             complement(<1..801)
                     /locus_tag="P5219_12905"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: protein motif:HMM:NF003742.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HRX27714.1"
                     /translation="MDLYVLSDCLSEVAEACASWIARRFDKTGIEITRFGSLSGEDRI
                     MEVLDEASGSDAVVLHCFLSGEFRRLVNKTCLHRGIDQYDAFSPVLSGVEQAAGKGAK
                     EDRALLHPMDSEYFRRVRAVEYTIAADDGGNPSILKEADVVIVGISRTGKSPLCMYLA
                     HRGVMAANVPLVPGIEPPPQLFRIHPSRIVGLIRSPGSLGEIRTRRMSMMGLAPEESA
                     YVRSEEILREIEYASGIMEHLGIVVFDVTRRAIEETAHEILALLAGAKE"
     gene            complement(869..>1017)
                     /locus_tag="P5219_12910"
     CDS             complement(869..>1017)
                     /locus_tag="P5219_12910"
                     /inference="COORDINATES: protein motif:HMM:NF015575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HRX27715.1"
                     /translation="AYATRERVERELRAATDYLKSLGARIYDVTDRAVEETAQEILDF
                     LRAR"
BASE COUNT          200 a          309 c          290 g          218 t
ORIGIN      
        1 ctctttggcc ccggcgagca gggcgagtat ttcgtgagcc gtttcctcga tggcccgtct
       61 ggtgacatcg aaaacgacga tgccgagatg ttccattata cccgaagcgt actcgatttc
      121 tctcaaaatt tcttcagaac gtacataggc gctttcttcc ggggccaatc ccatcatgga
      181 catgcgtctc gtcctgattt ctccgagaga gcccggagac ctgatcagcc ccacgatgcg
      241 ggagggatgg atgcggaaaa gctgcggagg gggctcgatt ccgggtacca ggggaacatt
      301 ggccgccatg actcccctgt gggcaaggta catgcagagc ggggacttcc cggttctcga
      361 tatgcccacg ataaccacat ccgcctcttt gaggattgag gggtttcccc cgtcatcagc
      421 ggcgatggtg tactccacgg cccgcacccg ccggaaatat tcgctgtcca tagggtgaag
      481 cagtgcccgg tcctctttcg cacccttgcc tgcggcctgc tccactccac tgagaacggg
      541 cgagaaagca tcatactggt caatgccccg gtgaaggcag gttttgttta cgagccgccg
      601 gaattctccg gaaagaaagc agtgaagcac tacggcatca gaaccggagg cttcgtcaag
      661 aacctccatg attctgtctt ctccggaaag ggagccgaac cgggttattt caatgcccgt
      721 cttgtcgaag cggcgggcaa tccaggacgc gcacgcctcc gctacttcgg agagacagtc
      781 cgaaagcaca tagagatcca cgttttcttc gtccgggaaa gaacagccgt tccgtcgttc
      841 accgaaggtg accacccgcc ttttcgtctc agcgcgccct gaggaagtcc agtatctcct
      901 gggcggtctc ttccaccgcc ctgtcggtta cgtcgtatat gcgggctccc agggacttga
      961 ggtagtcggt ggcggcccgc agttcccgct ccacccgctc cctggtggcg taggctg
//
LOCUS       DAOTHD010000974         1015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_181310_length_1015_cov_2.517708, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000974 DAOTHD010000000
VERSION     DAOTHD010000974.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181310_length_1015_cov_2.517708"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="P5219_12915"
     CDS             complement(<1..997)
                     /locus_tag="P5219_12915"
                     /inference="COORDINATES: protein motif:HMM:NF015905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HRX27716.1"
                     /translation="MSDREEASIWGTGRKTSVINAMMLNGSAINSFELDDTHTDGIIH
                     VSTGVLGCITAFAEMLGNMSGKDFLTAAALAYEISCRVAAPVGMEIAHQGWNNTGTCC
                     PFGSTAGVGNMLGLTPEQMGHAMGIAGNWSGGLQAVQFASMAKRIVPSKSSEGAIIGA
                     LLAQKGFTGIEDVFENKFGGFYNCFTDDVYDVERTSGELGNRWELMGIGLKFYSTCRS
                     KHTTIGALRKFREDHPDIQPDDIAKVVAHTTSITHKYSVDADAITSVVAAQLSHPYVC
                     AVTLLEGNAFIDQFTEEKIRNPRILEFAKKVDVVHDEEIEKLPRHLRYTVKVDVHL"
BASE COUNT          172 a          283 c          311 g          249 t
ORIGIN      
        1 tcaggtggac gtcaactttc accgtgtagc ggaggtgccg gggcagtttt tcgatctctt
       61 cgtcgtgaac cacgtccact ttcttcgcga actcgagaat ccttggattc cggatttttt
      121 cctcggtgaa ctgatcgatg aaagcgttcc cctcgagaag ggtgacggcg cacacgtatg
      181 ggtggctgag ctgggcggct acaacgctgg ttatggcgtc ggcgtccacg gagtacttgt
      241 gggtgatgga cgtggtgtgg gccacgacct ttgcgatatc gtcaggctga atgtcggggt
      301 ggtcctcccg gaacttccgg agtgcaccta tagtagtgtg cttgcttcgg caggtggagt
      361 agaacttgag gccgatgccc atgagctccc accggttccc gagctctccg ctggtgcgct
      421 ccacatcgta cacgtcatcg gtgaagcagt tgtagaatcc tccgaacttg ttctcgaata
      481 cgtcctcgat gcccgtgaat cctttctgtg cgaggagtgc cccgatgatt gccccttcgg
      541 atgacttgga cggaacgatc ctcttggcca tggaggcgaa ctggactgcc tggagtcctc
      601 ctgaccagtt ccccgcgatg cccatggcat gccccatctg ttcgggagtc aggccgagca
      661 tgtttcccac tccggcagtg gaaccgaagg ggcagcatgt tcccgtgttg ttccagccct
      721 ggtgggcgat ttccatgcct acgggtgcgg ctacccggca ggagatttcg taggccaggg
      781 ccgctgcagt gaggaagtct ttgccgctca tgtttccgag catttcggcg aaggcggtga
      841 tgcacccgag taccccggtg gagacgtgga tgataccgtc ggtatgggta tcatccagct
      901 cgaatgagtt tattgccgag ccgttgagca tcatggcgtt gatgacggac gtcttccggc
      961 ccgttcccca tatggacgct tcttccctgt cactcatgtt ctcgaggacg tcccg
//
LOCUS       DAOTHD010000975         1015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_181355_length_1015_cov_2.187500, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000975 DAOTHD010000000
VERSION     DAOTHD010000975.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181355_length_1015_cov_2.187500"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1015)
                     /locus_tag="P5219_12920"
     CDS             complement(<1..>1015)
                     /locus_tag="P5219_12920"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HRX27717.1"
                     /translation="FPVTVLLWCGIAVLIGRLMEWEPMARGSGIPQVEGELLGQVSMS
                     WWKVLAAKFIGGALAIALGLSLGREGPSVQIGAAAGKGMAVFQKRGKVEERLFLSSGA
                     SAGLSAAFNAPLAGVIFALEEVHKSFSPLVLMSAMMASLAADFVSKHFFGLRPVFSFG
                     VPSVLPLNLYGHLLLLGLLCGAGGALFCRVTLDVQTLYGRLPFPARYRVVIPALAALC
                     IGPLLPGILGGGHALVEHLGMGGEVLSVIFLLFAGKLFFTALSFGSGAAGGIFLPMLA
                     VGASLGALYGTAAARFTGVDQALVVNFLIVGMAGFFAAVVRAPITGIILITEMTGSFT
                     HLLS"
BASE COUNT          241 a          335 c          314 g          125 t
ORIGIN      
        1 ccgacagcag gtgagtgaaa gacccggtca tctcggtgat gaggatgatc cccgtgatgg
       61 gtgcccgcac aactgcggcg aagaaccccg ccattcccac gatgaggaag ttcacgacca
      121 gggcctggtc caccccggtg aaccgggccg ccgccgttcc gtacagggct ccaagggagg
      181 cccctacggc aagcatgggc agaaagattc cccccgcagc ccccgaaccg aaactgaggg
      241 ccgtaaaaaa gagcttcccc gcaaagagaa gaaaaatgac ggacaggacc tccccaccca
      301 tgccgaggtg ctccaccagg gcatgccccc ctccgagaat tccgggaagc agggggccta
      361 tgcaaagggc cgccagggcc ggaatgacca ccctgtacct ggcgggaaag ggcagtctgc
      421 cgtagagggt ctggacgtcg agcgtcaccc tgcagaagag ggcgccgccg gcgccgcaga
      481 gcagcccgag gagaagcagg tggccataga ggttcagggg aaggaccgag ggaacaccga
      541 aagaaaagac gggccggaga ccgaaaaagt gctttgacac gaaatccgcc gccagggagg
      601 ccatcatggc cgacatgagg accagggggg agaagctctt gtggacctcc tcaagggcaa
      661 agatcacccc cgcgagaggg gcgttgaagg cggctgaaag ccccgccgaa gccccgcttg
      721 acagaaaaag ccgctcctcc acctttcccc gcttttggaa gaccgccata cccttgccgg
      781 ctgcggcgcc gatctggacc gaaggcccct ccctgccgag ggaaaggcct agggcgatgg
      841 ccagtgcccc cccgataaat ttcgccgcca gcactttcca ccaggacatg gacacctggc
      901 cgaggagctc cccctccacc tggggaatgc cgcttcccct cgccattggc tcccattcca
      961 tcaggcgccc gatgagaaca gcaatcccgc accagagaag caccgtgacg ggaaa
//
LOCUS       DAOTHD010000976         1014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_181777_length_1014_cov_1.595412, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000976 DAOTHD010000000
VERSION     DAOTHD010000976.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181777_length_1014_cov_1.595412"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1014)
                     /locus_tag="P5219_12925"
     CDS             complement(<1..>1014)
                     /locus_tag="P5219_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HRX27718.1"
                     /translation="LRKLVQSIEDISVIRTESEVEALILEAKLIRKYSPFFNVDLKMS
                     DRYPYIKITRELFPRIVVTRQRGKDDALYFGPYVSAGDVRSLLRLIERYFPLRICARD
                     IDASTPEKNGRPCLHYSLGRCLGPCAGLVSPGEYRERVDDVILLLQGQSSEVVERLRK
                     RMDRAAKSLAFEEAARLRDTIRAIWRITRQRITSPLREDFDGDMWQCLNRLQQELRLP
                     VIPWRIDGFDISHMSGRETYGVVVVFEQGSPNTSLYRRFRIKTVEGVDDFRSLEETVT
                     RRYGQSLKGSEPLPQLVLIDGGPVQLEFARKALDELGLKDLPAVSLAKQEELIYHTPG
                     EEPL"
BASE COUNT          188 a          327 c          292 g          207 t
ORIGIN      
        1 aggggttcct cacccggagt atggtatatc agttcctcct gttttgccag cgatactgcg
       61 ggaagatcct tcagacccag ttcgtccaga gctttccggg caaattccag ctgcaccgga
      121 ccgccgtcga tgagcacgag ctggggcagg ggttcgctgc ccttcagaga ctggccgtag
      181 cggcgggtta ccgtctcctc cagggagcgg aaatcgtcca ccccctccac ggtctttatc
      241 ctgaaccttc tgtaaaggga ggtattcggc gagccctgct cgaacaccac caccacgccg
      301 taggtctccc ttcccgacat gtgggagata tcaaagccgt cgatcctcca ggggatcaca
      361 ggaaggcgga gctcctgctg gagcctgttc aggcactgcc acatatcccc gtcgaagtcc
      421 tccctcagtg gagaggtgat ccgctgccgg gtgattctcc agatggcccg gatcgtgtcc
      481 cgcagcctgg cggcctcttc gaatgccagg gatttcgccg ccctgtccat ccgcttcctc
      541 agccgctcca caacctcgga ggactggccc tgcagaagca ggatcacgtc gtccacccgt
      601 tccctgtatt cccccggact caccagcccg gcgcagggac ccaggcacct ccccagagaa
      661 tagtgaaggc atggcctgcc gtttttctcc ggcgtggagg cgtcgatgtc cctggcgcat
      721 atcctgaggg ggaaatatcg ctcgatgagc cggagcaggg aacggacgtc ccccgcgctc
      781 acgtaggggc cgaaatagag ggcatcgtcc tttcccctct gccgggtcac cacgatcctc
      841 gggaaaagtt cgcgggtgat cttgatgtag gggtaccggt ccgacatctt gaggtccaca
      901 ttgaagaagg gactatactt ccgtatcagc ttggcctcga gaatcagggc ctccacttcc
      961 gactcggtcc ggatgaccga tatgtcctct atggactgga ccagcttccg gagg
//
LOCUS       DAOTHD010000977         1011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_182503_length_1011_cov_2.228033, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000977 DAOTHD010000000
VERSION     DAOTHD010000977.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182503_length_1011_cov_2.228033"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..>1011
                     /locus_tag="P5219_12930"
     CDS             130..>1011
                     /locus_tag="P5219_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRX27719.1"
                     /translation="MKETTRAAYEEAVLRVQMHMHRTLDDPFDPNGIALVAGFSRFHL
                     SRLFSAMTGESLLEYRRRLLLERAAHSLLYRRHSVTELAFRAGYDTPEAFTRAFSKLY
                     GVPPGEYRRKLRAAMKHRLETIRARSKLCTLSLSLHEGGRKDMEVKIERHDSLKVAFI
                     RHVGPYEDCMEAWNRLCGAPGVTSRLGPGTLALSLCYDDPDVTEPDKIRMDVCVTIDD
                     SFVPGGGIDVQTIPGGEFAVVVYRGAYEGLHDAYRAIYGEWLPKSGREFGDSFSMEIY
                     RTDPGTTPPEENITEIRI"
BASE COUNT          212 a          295 c          323 g          181 t
ORIGIN      
        1 gaggacctgg gagatgacgg gctgactgac gcctccggcg gatgatcccg ttgtcctgaa
       61 aaaagcgcaa cgagagtcag gcgggaaggc aacggatgga gtatcctcag tcccgcagga
      121 gggaaaggca tgaaggaaac cacgcgcgcg gcgtacgaag aagcagttct ccgggttcag
      181 atgcacatgc acaggacgct ggacgatccg ttcgacccga acgggatcgc cctggtcgcc
      241 ggattctcca ggtttcacct ctcccgcctc ttctcggcga tgacggggga atccctgctt
      301 gagtaccgga ggcggctgct gctggaacgg gctgcccaca gcctgctcta caggcggcat
      361 tccgtgaccg agctggcctt ccgggccgga tacgacactc cggaggcatt tacgagggca
      421 ttctcgaagc tctacggtgt ccctcccggc gagtaccgcc ggaagctgcg ggcagccatg
      481 aagcaccgcc ttgagacgat ccgggcccgg tcgaagctct gcaccctttc cctctcctta
      541 cacgaaggag gaagaaaaga catggaagtg aagatcgaac ggcacgactc tttgaaagtt
      601 gcgtttatcc gtcacgtggg tccctatgag gactgcatgg aggcatggaa caggctctgc
      661 ggggctcccg gagtgacgtc ccgtctcggg cccggtacgc tggccctctc gctctgctac
      721 gacgatcctg acgtgacgga gccggacaaa atcaggatgg atgtctgtgt caccatcgac
      781 gacagttttg ttcccggcgg cggcatagat gttcagacca ttcccggggg agagtttgcc
      841 gtcgtggtct acaggggagc ctacgaaggc ctgcacgacg cctaccgggc catatacggc
      901 gagtggcttc cgaaaagcgg cagggaattc ggtgattcct tctccatgga aatctaccgg
      961 acggaccccg gcacgacgcc gccggaggag aacattacgg agatacggat a
//
LOCUS       DAOTHD010000978         1011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_182532_length_1011_cov_2.069038, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000978 DAOTHD010000000
VERSION     DAOTHD010000978.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182532_length_1011_cov_2.069038"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..166
                     /locus_tag="P5219_12935"
     CDS             14..166
                     /locus_tag="P5219_12935"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HRX27720.1"
                     /translation="MGVIRPQDVDSYNQISSDKIANAEIGVKGKGALTRLQKPGILTQ
                     ILQTIF"
     gene            180..>1011
                     /locus_tag="P5219_12940"
     CDS             180..>1011
                     /locus_tag="P5219_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HRX27721.1"
                     /translation="MKLMGRIAAVFLLLLLAASMSAGAEIHPTVRIKDLADVEGVRSN
                     QLVGVGIVMGLQGTGDKGTMATQMIRNLMSQFGVTLNDKAVKSKNVAVVTITADLPPF
                     ARPGQRIDVTVSTMGDAKSLQGGTLLQVPLKGADGAVYAVAQGPVMVGGFAAAGAAAS
                     VSKNVVTVGRVPGGAIVERDVQTDFTLMGRQVSLLLRNPDFTTSDRMARAINSAFGAV
                     AFAVDAGRVAVNVPPQYAASPASFLARIEGLSLRPDGAAGVAVNERTGTVVMGGNVRI
                     S"
BASE COUNT          187 a          332 c          326 g          166 t
ORIGIN      
        1 ccagctccag gtggtggggg tcatccgtcc ccaggacgtg gacagctaca accagatatc
       61 gagcgacaag atcgccaacg cggaaatcgg cgtcaagggc aagggagcgc ttacccgcct
      121 ccagaagccc ggaatcctga cccagatcct gcagaccatt ttctagggag gagcggaaca
      181 tgaaactcat gggcagaatc gctgcggtct ttctcctcct tctcctggcg gcctccatgt
      241 ccgcaggtgc ggaaattcac cccaccgtcc ggataaagga cctcgccgac gtggaaggcg
      301 tccggtcgaa ccagctcgtt ggcgtaggca tcgtcatggg ccttcagggc acgggcgaca
      361 aggggaccat ggcgacccag atgatacgga acctcatgag ccagttcggc gtgaccctga
      421 acgacaaggc cgtgaagagc aaaaatgtgg cggtggtcac cattaccgcc gatcttcccc
      481 ccttcgcccg tcccgggcag aggatcgacg tcaccgtgag caccatgggt gacgccaaga
      541 gcctccaggg gggcaccctg ctccaggttc ccctcaaggg ggccgacggc gctgtgtacg
      601 ccgtggccca gggtcccgtc atggttggcg gattcgccgc cgccggcgct gcagcatcgg
      661 tgtcaaagaa cgtggtcacc gtggggcgcg ttcccggagg cgccattgtc gagcgggacg
      721 tccagacaga cttcaccctg atgggcaggc aggtcagcct cctgctgcgg aaccccgact
      781 tcaccacctc cgaccggatg gcccgggcca tcaacagcgc cttcggcgcc gtggcctttg
      841 ctgtggatgc cggacgggtg gccgtcaacg tcccgcccca gtatgcagcg tcccctgcct
      901 ccttccttgc ccggatcgag ggactgtctc tccggcccga cggcgcggcc ggggtggcag
      961 tcaacgaacg gacgggcacc gtggtcatgg gcgggaacgt gaggatcagt t
//
LOCUS       DAOTHD010000979         1008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_183400_length_1008_cov_2.332634, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000979 DAOTHD010000000
VERSION     DAOTHD010000979.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183400_length_1008_cov_2.332634"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1008
                     /locus_tag="P5219_12945"
     CDS             <1..>1008
                     /locus_tag="P5219_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27722.1"
                     /translation="GSLGKDTLERLSPYLVRPGEPGSVFEGDGEDAEAGRSFSAGPAE
                     AADRPSGYTYSAKCTRAPITVWSGPKNAVKARRVRDLVDSHDMYGKFLDLMGKRPPDD
                     NGEAGNSTNPDSRLDIFLVRMDSSAGSVCGVCRATSLGKKVSPSVILINNTLEGDLLA
                     STLAHELFHAFQNAYSVFEEKWLSEGTAVWAEDFIGSGWNTEQEYLPALFARDSNRME
                     TLTGSDGDAPYGAYLFPFFLTKHAFTSRGRDADKIVGEIWHAAGADDDFQSLRSVNHA
                     LKGKFDEHFRKFALVTMDLPPFDGIYPKSENLEIRPLHREEVWLLYEPGEQTVEIPLP
                     PL"
BASE COUNT          214 a          276 c          318 g          200 t
ORIGIN      
        1 cggatcattg gggaaggata ccctggaacg gctgagcccg tatcttgtcc ggcccggtga
       61 accggggagc gtgttcgaag gcgacggaga ggatgcagaa gccggccgga gtttttcagc
      121 cggtccggcg gaggccgcag accgtccctc cggatatacc tattcggcca aatgcaccag
      181 ggcgccgatc accgtgtggt ccggacccaa aaatgcggtg aaggcacgaa gggtaaggga
      241 cctggtggat tctcacgata tgtacggtaa gtttctggac ctgatgggga aaaggccgcc
      301 cgatgataac ggggaggccg gaaacagcac gaatcctgac agcaggctgg acatttttct
      361 ggtccgcatg gattcgtcgg ccggcagcgt ctgcggggtc tgccgggcca cgtctctggg
      421 aaagaaggtc tcgccgtcag tgatactgat caacaatacg ctggaagggg atcttcttgc
      481 ttcgaccctg gcccatgagc ttttccacgc attccagaac gcctattccg tctttgagga
      541 gaagtggctg tcggagggaa cggcagtctg ggccgaggac ttcataggaa gcggctggaa
      601 tacggagcag gaatatttgc ccgccctttt tgcccgggac agcaacagga tggaaacgct
      661 cactggttct gacggggacg caccgtacgg ggcgtatctt ttccccttct ttctcaccaa
      721 gcatgccttc acgtcccggg ggagagacgc ggacaaaata gtgggcgaaa tatggcatgc
      781 cgcgggtgcg gacgatgatt ttcagtctct ccggtctgtg aaccacgccc tcaagggaaa
      841 gtttgacgag cacttccgta agttcgccct tgtgaccatg gacctgcctc ccttcgacgg
      901 gatttacccg aagagcgaaa acctggaaat ccggcccctt caccgggagg aggtatggct
      961 gctctacgag ccgggggaac agaccgtgga aattcccctt ccgcccct
//
LOCUS       DAOTHD010000980         1006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_183937_length_1006_cov_2.895899, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000980 DAOTHD010000000
VERSION     DAOTHD010000980.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183937_length_1006_cov_2.895899"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..>1006
                     /gene="sat"
                     /locus_tag="P5219_12950"
     CDS             20..>1006
                     /gene="sat"
                     /locus_tag="P5219_12950"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003166.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HRX27723.1"
                     /translation="MLTLNEREKNDLEMIASGAMSPLEGFMTREDYGSVVDDMRLPGG
                     QVWTLPVVFAAKDGDPAVKPGDTVNLAWEGRIIGTMEVADVFTADLMHEAEETLLTTD
                     DAHPGVAYLKSLSGTYIGGRTMALDTRDEEPFREHRLTPAETRALFREKGWKSIVAFQ
                     TRNPIHRAHEYLQKVSLELVDGLLVHPLVGATKSDDISAEVRMRCYETILEKYYPADR
                     TALSVFPAAMRYAGPREAVFHAILRKNYGCTHFIVGRDHAGVGDYYGTYDAQEIFDRF
                     SPEEIGIVPMKFEHAFFCNACDGMATARTCPHTADDRVFLSGKKVRAMLQNGE"
BASE COUNT          203 a          323 c          313 g          167 t
ORIGIN      
        1 cattccggat ggggctcctg tgcttaccct gaacgagcgg gagaagaacg acctggagat
       61 gatagcctcc ggggccatga gcccccttga aggcttcatg accagggagg actatggttc
      121 cgtggtggat gacatgcgcc tgccgggggg acaggtatgg accctcccgg tggttttcgc
      181 cgccaaggac ggtgatcccg ccgtgaagcc cggcgacacg gttaaccttg cctgggaggg
      241 acggatcatt ggcaccatgg aggtggccga cgtcttcacc gcggacctta tgcacgaagc
      301 tgaggaaacc cttctcacca ccgacgacgc ccaccccggt gtggcctatc tgaagagcct
      361 ttccggtacc tatatcgggg gcaggacgat ggccctggac accagggacg aagagccctt
      421 cagggagcac aggctcaccc cggcggagac cagggccctc ttcagggaga agggctggaa
      481 gagcatagtc gccttccaga ccaggaatcc catccacagg gcccacgagt acctgcagaa
      541 agtctccctg gagctggtgg acggcctgct ggtgcatccc ctcgttggcg ccaccaaatc
      601 cgacgacatc tccgcggagg tccggatgcg ctgctacgag accatcctgg agaagtacta
      661 tcccgcggac cgcaccgccc tcagcgtttt ccccgccgcc atgaggtacg ccgggcccag
      721 ggaggcggtt ttccacgcga tcctgaggaa gaactacggc tgcacccact tcatcgtggg
      781 aagggaccac gccggggtcg gggactacta cggcacctac gacgcccagg agatattcga
      841 ccgcttctcc ccggaggaga tagggatcgt tcccatgaag ttcgagcacg ccttcttctg
      901 caacgcctgc gacggcatgg ccaccgccag gacctgtccc cacaccgccg atgacagggt
      961 cttcctctcc ggcaagaagg tccgggcaat gcttcagaac ggggaa
//
LOCUS       DAOTHD010000981         1006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_183950_length_1006_cov_2.731861, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000981 DAOTHD010000000
VERSION     DAOTHD010000981.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183950_length_1006_cov_2.731861"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..>1006
                     /gene="htpG"
                     /locus_tag="P5219_12955"
     CDS             2..>1006
                     /gene="htpG"
                     /locus_tag="P5219_12955"
                     /inference="COORDINATES: protein motif:HMM:NF003555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HRX27724.1"
                     /translation="MPVLSESAAQAENEGESRLSAPTIRISRGEEGRTLFVSDNGVGM
                     NREELTNYIGTIARSGTGEFLSAMKAARESAGAESLIGRFGVGFYSAFMVADRVDVVT
                     RRLGEGKGWRFSSPGDGTYMLEEAERNEPGTTVILHLKTPDAEKGERDYTDEWTLRDI
                     VRKYSDFISYPIVMAVSKKTEDGEAAEDQVLNSGKAIWRRPEKEVTDQEYEEFYKHVA
                     SDWEPPLGRVVFSVEGGTEFRGILFFPSRAPFDLQYAPRSEGVSLYIRNVFIMNDCRD
                     LVPSWLRFLRGVVDSEDLPLNISREILQEDPLVRIIRKSLVRRVLASLKKMMADDREK
                     Y"
BASE COUNT          232 a          272 c          305 g          197 t
ORIGIN      
        1 attgccggtt ttaagcgaaa gtgccgcaca agccgaaaac gaaggggaat cccgcctgtc
       61 ggcacccact atcagaattt cgaggggtga ggaaggacgg actctttttg tctccgacaa
      121 cggcgttggg atgaacaggg aggaactgac gaactatatc gggaccatcg ccaggtccgg
      181 aaccggggaa ttcctttcgg ccatgaaagc cgccagggaa tcggcggggg ccgagagcct
      241 gatcgggcgg ttcggagtgg gattttattc cgccttcatg gtggctgacc gtgtggacgt
      301 ggtgacccgc cgtctcggcg aaggaaaagg ctggcgtttc tcttcccccg gagacggcac
      361 ctatatgctg gaagaggctg aacggaacga accgggaact actgtgatcc ttcacctcaa
      421 gacgcccgac gcggagaaag gggaacggga ttacaccgac gagtggaccc tccgggacat
      481 tgtcagaaaa tattccgatt tcatctccta tcccatcgtg atggccgttt cgaaaaagac
      541 agaagacggc gaggcggcgg aggaccaggt tctcaactcg gggaaggcca tctggcgtcg
      601 cccggaaaaa gaagtgaccg accaggaata cgaagaattc tacaagcatg tcgcctccga
      661 ctgggaaccg cccctcggca gggtggtttt ttccgtggag ggaggaactg aattccgggg
      721 aatcctgttt ttcccgtcga gagccccctt cgatcttcaa tacgctcccc ggagcgaggg
      781 agtgagcctc tacatccgaa acgtcttcat catgaacgac tgccgggacc ttgtcccctc
      841 ctggctccgc ttcctgaggg gcgtggtgga ctccgaggat ctcccgctca acatctccag
      901 ggaaattctc caggaggatc ccctcgtccg gatcatcagg aagagccttg ttcggcgtgt
      961 tcttgcatcg ctgaaaaaaa tgatggccga tgacagggag aaatac
//
LOCUS       DAOTHD010000982         1006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_184034_length_1006_cov_2.052576, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000982 DAOTHD010000000
VERSION     DAOTHD010000982.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184034_length_1006_cov_2.052576"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="P5219_12960"
     CDS             <1..509
                     /locus_tag="P5219_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRX27725.1"
                     /translation="PKSIGYGFTTVQKYPISLEIAGGPKPVSMDIGITKGAIFRGRLA
                     LREGGTGDGGKEGAVGPPATESGAKPVSLAHILVELTRDDQTVRRSTDMNGEFVFDNI
                     RPGNWQLTFYEAGIPAGYQFETESKTIDLAPGDVMEMVNHIFRKKRTIQFIDSGTVTT
                     TSSSGKKK"
     gene            536..>1006
                     /locus_tag="P5219_12965"
     CDS             536..>1006
                     /locus_tag="P5219_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27726.1"
                     /translation="MMISRSGSGSLPILSILLACAAGALLLGAMDFPSEAAESAALSL
                     EVTIPEFRGVHLTNTSVSMAAMIGGKPGESAFFFLLSSTGDAPRKLTGRLVSPLPAGL
                     SLTVEITSPREGRSTGPQSLGTGEVDLLTGFQGLFGAGCRGTMKMMGNNSLAPGA"
BASE COUNT          213 a          286 c          318 g          189 t
ORIGIN      
        1 accccaagtc catcggctac ggtttcacca cggtgcagaa gtaccccatt tccctcgaga
       61 tcgcgggagg gcccaaacct gtctccatgg atatcggcat aaccaaaggg gcgattttca
      121 ggggaaggct cgctctcagg gagggaggaa cgggagatgg cggaaaggaa ggtgccgtgg
      181 gaccgccggc gacggaatcc ggcgccaagc ctgtcagcct ggcccacata ctggtggagc
      241 tgacaaggga tgaccagaca gtcagacgtt ccaccgacat gaacggggag ttcgtgttcg
      301 acaacatccg ccccgggaac tggcagctga cattttacga agcgggcatc cccgccggct
      361 accagttcga gaccgagtcg aaaaccattg atctggcgcc tggggacgtg atggagatgg
      421 tgaatcacat tttccggaag aaacggacca tacagtttat cgacagcggc accgttacga
      481 cgacgtcctc ttccggaaaa aagaaatgaa ccctcgggga aaggggaagg gcacaatgat
      541 gatttctcgg tccggttccg gatcgcttcc gattctttcg attcttctcg cgtgcgccgc
      601 gggagcgctg ctgctggggg ccatggattt cccctcggag gcggcggaat cggccgcgct
      661 ttccctggaa gtgaccattc cggaattccg cggggtccat ctcacgaaca cttccgtctc
      721 aatggccgcc atgatcggcg gaaaaccggg cgagagcgcc tttttctttc tcctttcctc
      781 caccggggat gcgccgagaa agctgacggg ccgtcttgtg tcgcctctgc cggcagggct
      841 gtcgctgacg gtggagataa cttcaccaag ggaagggcgg tccaccggac cccagtctct
      901 cggtaccgga gaggtcgacc tcctgacggg atttcagggc ctatttggtg cgggatgcag
      961 gggaacgatg aaaatgatgg gcaacaacag ccttgcgccc ggggcg
//
LOCUS       DAOTHD010000983         1005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_184161_length_1005_cov_3.807368, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000983 DAOTHD010000000
VERSION     DAOTHD010000983.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184161_length_1005_cov_3.807368"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="P5219_12970"
     CDS             complement(<1..386)
                     /locus_tag="P5219_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX27727.1"
                     /translation="MSKITLKNVTKEFVTRGGHRVTAVDNFNLEIREGECFSFLGPSG
                     CGKTTTLRMIAGFEDLTEGEIWLGDKPVSVKSKNLYVPPEERGLGMVFQAFAVWPHLN
                     VFENVAFPLRVKKTPRDELRERVMTAL"
     gene            complement(388..>1005)
                     /locus_tag="P5219_12975"
     CDS             complement(388..>1005)
                     /locus_tag="P5219_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRX27728.1"
                     /translation="RDSIMNSLFLSVSSGVITMFLGVMIAYVVIKVKPRGKGFLEMLS
                     LLPYSIPGLVLAIGVILVWSGAFGINLYNTIWIILIAYIARYVSFAMKSASASLEQVH
                     YSLEEAARSCGATHLESLRDITIPLIRPGMIAGFFLIFLPAMRELTTSVLLYGPFTRT
                     LGVAIYAINSEGNTVQASALATVAIGIIIAGHFALRYVTRDRKGL"
BASE COUNT          201 a          294 c          325 g          185 t
ORIGIN      
        1 agggcggtca tcacccgctc ccggagctcg tccctcgggg tcttcttgac cctcagggga
       61 aaggcaacgt tctcgaagac gttcaggtgg ggccacacgg cgaaggcctg gaagaccatg
      121 cccagccccc gctcctccgg cgggacgtag aggttcttcg atttcaccga cacgggcttg
      181 tcacccagcc agatttcccc ttccgtcagg tcctcgaacc cggcgatcat gcggagggtg
      241 gtggtcttcc cgcagccgga gggaccgagg aaggagaagc actccccttc ccggatctcg
      301 aggttgaagt tgtccaccgc tgtcacccgg tggcctcccc gggtgacgaa ttccttggtc
      361 acgttcttca gcgtaatctt gctcatgtta taaacccttc ctgtctctgg tcacataccg
      421 gagggcgaag tgccccgcga tgatgatccc gatggccact gtggcaaggg ccgaagcctg
      481 caccgtgttc ccttcggagt tgatggcgta gatggccacg ccgagggtcc tggtgaaggg
      541 cccgtagagc agcaccgacg tggtcagctc cctcatggcc ggcaggaaga tgaggaagaa
      601 ccctgcgatc atgcccggac ggatcagggg tatggtgatg tcccggaggg actcgaggtg
      661 ggtggccccg caggaccggg ccgcctcctc cagggagtag tggacctgtt caagggaggc
      721 cgacgccgat ttcatggcga aggaaacata ccgggcgata taggcgatga ggatgatcca
      781 gatggtgttg tagaggttga tcccgaaggc gccgctccag accaggatga cgccgatggc
      841 gagcaccaat cccggaatcg agtagggcag cagggagagc atttccagga agccctttcc
      901 ccggggtttc accttgatga ccacgtaggc gatcatgacg cccaggaaca tagtgatcac
      961 gcccgaggag accgagagga agaggctgtt cattatggaa tcccg
//
LOCUS       DAOTHD010000984         1003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_184771_length_1003_cov_2.944093, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000984 DAOTHD010000000
VERSION     DAOTHD010000984.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184771_length_1003_cov_2.944093"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="P5219_12980"
     CDS             complement(<1..822)
                     /locus_tag="P5219_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HRX27729.1"
                     /translation="MSLKYAIEAYELLDSPSASGESVREALIAAGVSAGSITVKKVEG
                     PKGHTDFIKVWFSGKSGKRGGGTAPTLGIVGRLGGIGARPERIGLVSDGDGAVTAVAA
                     AMKLGRMASLGDVLAGDVFISTHICPNAPTRPHDPVPFMDSPINMAIANGEEMSDELD
                     AVLSIDTTRGNRVINCRGFALSPTVREGYILRVSEDLLSLMASVTGRAPVVFPITTQD
                     ITPYGNGLYHLNSILQPCTATKAPVVGIALTAETAVPGCATGSSQVVDIEAAVRFA"
BASE COUNT          153 a          331 c          321 g          198 t
ORIGIN      
        1 ggcgaaccgc accgccgcct cgatatccac cacctgggac gatcccgtgg cgcaccccgg
       61 aacggccgtt tccgccgtca gagcgatccc caccaccggc gccttcgtgg cggtgcaggg
      121 ctgcagtatg ctgttcaggt ggtagaggcc gttgccgtag ggcgtaatgt cctgggtggt
      181 gatggggaac accactggag cccggccggt gacgcttgcc atcagggaga gaaggtcttc
      241 gctcaccctg aggatatacc cctccctcac cgtaggggag agggcgaacc ccctgcagtt
      301 gatcacccgg tttcccctgg tggtgtcgat ggagagcacg gcgtcgagtt cgtcgctcat
      361 ttcctcgccg ttggcgatgg ccatgttgat gggcgagtcc atgaagggga ccgggtcgtg
      421 gggccgggtg ggggcgttgg ggcagatgtg ggtggagatg aagacgtcac cggccaggac
      481 gtcccccagg ctcgccatcc ggccgagctt catggccgcc gccaccgccg tgacggcgcc
      541 gtccccgtcc gagacgaggc ctatccgctc cggccgggcg ccgattcctc ccaggcgccc
      601 cacgatgccg agggtgggag cggttccccc tccccgtttt ccgctctttc cggagaacca
      661 gaccttgatg aagtccgtgt ggcctttcgg tccctccact tttttcacgg tgatgctgcc
      721 ggcgctgacg cctgcggcaa tcagggcctc ccgcaccgac tcccccgacg ccgacgggct
      781 gtcgaggagt tcgtaggctt cgatggcata tttcagggac atgagatctt cccctcctat
      841 ctgatgtatt cgactatttt ctttcccacc acgggctcga tttcctccat gagttccatg
      901 caccgcatgc cctggcccag gatgagccgg tcggcgggga cgacgaaaag aatgacggtc
      961 tgcccttcct ttacttccgc cgtggtgacg ggaagcccgg acc
//
LOCUS       DAOTHD010000985         1003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_184934_length_1003_cov_1.715190, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000985 DAOTHD010000000
VERSION     DAOTHD010000985.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184934_length_1003_cov_1.715190"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1003)
                     /locus_tag="P5219_12985"
     CDS             complement(<1..>1003)
                     /locus_tag="P5219_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX27730.1"
                     /translation="KLDPEGSKKRQEEAANISDAMTPVFQDGRNELLYQVGGLIHRVA
                     NGKSSVPVTARYRDVSDLFHFTIDGTYSDPSTRGDIPVELVVDTQTYGIVAMTSVVFT
                     ENGVAISDVTPSPEGVFKPSIIKFGPDGKVKIVPGEAIRWEEGLDVILEMIPAGKTLR
                     IIGQAESIGGAGTSLVSPPVTVAANPEITPRLDETHRLGTEKLPGKYAAFTAVAQRNG
                     QGMIIAPAGNVITISQAGKNPNVFPAKAEVFGEKPSEFALLWEPRGLPILTGYAKEEP
                     EGEYQPAERRFAVLAIEGKSWFWTTFDSYTMAKTIMMPLDEFNFPAGYMEGNWKGDDG
                     "
BASE COUNT          152 a          310 c          307 g          234 t
ORIGIN      
        1 ccgtcgtcgc ctttccagtt tccttccatg tagccggcgg gaaagttgaa ctcgtccagg
       61 ggcatcatga tggtcttcgc catggtgtag ctgtcgaagg tggtccagaa ccagcttttt
      121 ccctcgatgg caagaacggc gaaccggcgc tccgcgggct gatactcgcc ttcgggctcc
      181 tcctttgcgt atcccgtcag gatgggcagt ccccggggct cccacaggag ggcgaattcc
      241 gacggcttct ccccgaaaac ctcagccttc gccgggaata cgttcggatt cttccctgcc
      301 tggctgatgg tgatcacgtt tccggccgga gcgatgatca tcccctggcc gttccgctgc
      361 gccacggcag tgaaggccgc atacttcccg ggcagcttct ccgttcccag gcggtgggtt
      421 tcgtcaagcc tcggggtaat ttccgggttg gccgccacgg tgacgggagg gctcaccagg
      481 ctggttcccg ctccgccgat ggattcggcc tgcccgatga tccgaagggt ttttccggcg
      541 gggatcattt cgaggatgac gtcgaggcct tcttcccacc gtatggcttc tcccggtacg
      601 atctttacct tgccgtcggg tccgaacttg atgatcgagg gcttgaacac tccttcgggg
      661 ctgggcgtca cgtcggagat ggccacgccg ttttccgtga agaccaccga cgtcatggca
      721 acaatgccgt aggtctgggt gtccaccacg agctccaccg ggatgtctcc ccttgtggag
      781 ggatcggaat atgtgccgtc gatggtgaag tggaacaggt cggacacgtc cctgtaccgt
      841 gcggtgacgg gaacgctcga ttttccgttg gccaccctgt ggatgaggcc cccgacctgg
      901 tagagcagct cattccggcc gtcctggaag accggcgtca tggcgtcgga gatgttcgca
      961 gcttcttcct gccgtttctt gcttccctcc ggatcgagct ttt
//
LOCUS       DAOTHD010000986         1002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_185038_length_1002_cov_3.470961, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000986 DAOTHD010000000
VERSION     DAOTHD010000986.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185038_length_1002_cov_3.470961"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="P5219_12990"
     CDS             complement(<1..382)
                     /locus_tag="P5219_12990"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HRX27731.1"
                     /translation="MLRVLPYSWSISLGAGLGALLWAASKKKVDEAERRCVKALQVGV
                     TEARRIVRGSYMNLGRSVAEFLSMDKVRGDLRDLVEFHGEEHLREALARGKGVLFLSA
                     HLGNWEIGAAAVAERGYPMNAIGTD"
     gene            523..>1002
                     /locus_tag="P5219_12995"
     CDS             523..>1002
                     /locus_tag="P5219_12995"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRX27732.1"
                     /translation="MLTRILFLADVSPFSERALAWASEKLKGQQTDFLVLHVVDPAAG
                     MEAPHVVREAEGYLEEMAARVLPPESYYKTLVLSGDILESLPETVRSEGCTFALLALP
                     ERVDGLPLIRSIPVPQLVIRESEGQLPDTGVFGKLALALDLEPNRTSLMLDNLRAMLS
                     "
BASE COUNT          203 a          320 c          261 g          218 t
ORIGIN      
        1 ggtccgtccc gatggcgttc atggggtatc ccctttcggc gacggcggcc gctccgatct
       61 cccagtttcc cagatgagcg gagagaaaga ggaccccttt cccccttgcc agggcctcac
      121 ggagatgctc ttcaccgtga aattccacca ggtcccgcag atcgccccgc accttgtcca
      181 tggaaaggaa ttcagccact gaacgtccaa ggttcatata ggatccgcga acgattctcc
      241 tggcctcggt caccccgacc tgcagtgcct tcacgcatct cctttccgct tcgtccacct
      301 tttttttgct cgccgcccag agaagggcgc cgaggccggc gccgagggag atcgaccatg
      361 aatagggcaa aaccctgagc acattccgga taccgcctgc aatcttccac gaagtatccc
      421 tattcatgct caataccgca tctttcgaat tttctccgca ctgaaaagac tcctttacga
      481 tatacttgtc cggaagacca gaataccatg ggagggatga tcatgctcac acggattctt
      541 tttctcgccg atgtttcccc cttttccgaa cgggccctgg cctgggcgtc ggagaagctg
      601 aagggacaac agacggattt tctcgtgctt cacgtggtcg atcccgccgc aggaatggag
      661 gctccccacg tggtgcggga ggcggaggga tacctcgaag aaatggcagc ccgggttctc
      721 ccccccgaat cctattataa aaccctggtt ctgtccggcg acatcctgga gtccctgccg
      781 gaaacggtcc gctccgaagg atgcaccttt gcacttctcg cattgcccga aagagtggac
      841 ggactcccgc tgatacggtc cattcccgtt ccccagcttg taattcggga atcggaagga
      901 cagctgcccg acaccggggt tttcgggaag cttgccctgg ctcttgatct ggagcccaac
      961 aggaccagcc tgatgctcga caacctcagg gcaatgctgt ca
//
LOCUS       DAOTHD010000987         1001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_185360_length_1001_cov_3.026427, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000987 DAOTHD010000000
VERSION     DAOTHD010000987.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185360_length_1001_cov_3.026427"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..849
                     /locus_tag="P5219_13000"
     CDS             109..849
                     /locus_tag="P5219_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX27733.1"
                     /translation="MTRDLKFSRKTASSIGEAIADSKMISRGDRITVGLSGGKDSVLL
                     AAALAGLTKRSPVPFTVDACMIDITGGKTDTAPVRTLCEEIGIDLEIISRPVLDIIAS
                     RKEPSPCSFCSNMRGGILFRRAAEKGSTTVAMGHNLDDAVETALLNLFFAGRFDCFAP
                     KSWRSRSGLWLIRPLVYIQEKDIEKEVQRLDLPTISPMCPFPRESRRERMKKLVGILE
                     EEIPDIRSQVLHALKGSPRWKEFSQKMQ"
BASE COUNT          259 a          267 c          268 g          207 t
ORIGIN      
        1 ttccttccgg tgccagtcag aaaacggact gaacctacaa agggcattat aacccatcat
       61 tgccaatgat gcagatacca gactccggtt gggagtgaag acccgaaagt gacaagggac
      121 ctgaagttct caagaaaaac ggcttcgagc atcggagagg ccattgctga ttcaaaaatg
      181 atatccaggg gagacaggat cacggtggga ctctcgggag gcaaggacag tgtgttgctt
      241 gcggctgccc ttgcgggcct gacgaaaagg agtcccgtcc cctttaccgt tgacgcctgc
      301 atgatagaca ttaccggggg aaagaccgat acggccccgg taaggacgct atgtgaagag
      361 attggcattg atcttgaaat tatctcccgg ccggtacttg atattatcgc atcccgtaaa
      421 gaaccttccc cctgcagctt ctgctccaac atgagggggg ggatactctt caggcgggca
      481 gcggaaaaag ggtcaacaac ggtcgcgatg ggtcacaacc ttgacgatgc cgtggaaacg
      541 gcactgctga atctcttctt cgccggcagg ttcgactgtt tcgctccaaa atcctggagg
      601 agccggagcg gcctgtggct cataaggcca ctggtttata tccaggagaa agatatcgaa
      661 aaggaggtac agaggctgga ccttcccaca atatccccca tgtgcccctt cccgcgagag
      721 agcagaaggg aaagaatgaa aaaacttgtc ggaatcctcg aagaagaaat tcccgatata
      781 aggtcccagg tcctgcacgc attgaaagga agcccccgat ggaaggaatt ttcccaaaaa
      841 atgcagtaaa cccgttatct gccgttctcc ggaaaacccc tctccagccc cctcaccagt
      901 agcgttatca ccctggtaca ggggaaatcg ataccgtgtg cctcgccggc cgcaaggatc
      961 tgcccgttta tgttgtcaat ttccgttcgt cggcccgctt c
//
LOCUS       DAOTHD010000988         1001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_185382_length_1001_cov_2.671247, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000988 DAOTHD010000000
VERSION     DAOTHD010000988.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185382_length_1001_cov_2.671247"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="P5219_13005"
     CDS             <1..574
                     /locus_tag="P5219_13005"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRX27734.1"
                     /translation="VVADITADNFAMGAKASTFLMDTIGGQGNIIVIKFEKHYGTRRR
                     GKVLDVVLTEYPGVKVLAEYSVVATKRFMDDTRAAMETYATRFGDQIDGVWCAFDQLA
                     YVAGDVLQERGNKKAVIVGVDGNQETFRRIGAGQMAATVAQPFEAMAGKAIELVNSLA
                     VEGKTVEEATGGRKIFYMDAPLIDRTNLPE"
     gene            659..>1001
                     /locus_tag="P5219_13010"
     CDS             659..>1001
                     /locus_tag="P5219_13010"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRX27735.1"
                     /translation="MPLFLGVDMGTSSLKACVIEGEGHTAARAKVPSETHSPAPGMYE
                     VDGEACWWRGFLSLCGEISRSVPLSDIAGICVSSVCGSFIPVDGEGRQTHNAILYGID
                     RRLDLFEQRFAF"
BASE COUNT          206 a          288 c          328 g          179 t
ORIGIN      
        1 cgtggtggcg gacatcacgg cagacaactt cgccatgggg gcgaaggcgt ccaccttcct
       61 gatggacacc atcggaggcc agggcaatat catcgtcatc aagttcgaga agcactacgg
      121 cacccgccgg aggggaaagg tcctcgacgt ggtgctcacc gagtaccccg gagtgaaggt
      181 cctggccgag tacagcgtgg tggctaccaa gcggttcatg gatgacacca gggcagccat
      241 ggagacctac gcgacccgtt tcggcgacca gatcgacggc gtgtggtgcg ccttcgacca
      301 gcttgcctac gtggcaggtg acgtacttca ggagcggggg aacaagaagg cagtaatcgt
      361 cggtgttgac ggcaaccagg agaccttccg gcggatcggt gccggacaga tggccgccac
      421 ggtggcccag cccttcgaag ccatggcggg gaaggccatc gagctggtga attccctcgc
      481 cgtggaggga aaaaccgttg aggaagccac cggagggcgg aagatcttct acatggacgc
      541 tcctctcatt gacaggacca acctgccgga ataacatccc ttaagggaaa gaaaagcagc
      601 acaccgcggg ccgccggaag atctttccgg gcggcccgtt tcaaaggaga tgaagcctat
      661 gcccctgttt ctgggagtgg atatgggaac gtcctccctc aaagcctgtg tgatcgaagg
      721 agaaggccat acggccgcga gggcgaaggt cccgtcggag actcattctc ccgctccggg
      781 aatgtacgag gtggacggag aagcatgctg gtggcggggc ttcctttccc tctgcgggga
      841 gatttcccgc tccgttcccc tgtcggatat tgccgggatc tgcgtcagtt cagtgtgcgg
      901 gtccttcatc ccggtggacg gcgaagggcg ccagacccac aacgccatcc tgtacggcat
      961 tgaccggcgc ctcgacctgt ttgagcagcg atttgccttc g
//
LOCUS       DAOTHD010000989         1001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Aminivibrio sp. isolate AMR_MDS_3060
            NODE_185484_length_1001_cov_2.006342, whole genome shotgun
            sequence.
ACCESSION   DAOTHD010000989 DAOTHD010000000
VERSION     DAOTHD010000989.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868084
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aminivibrio sp. (wastewater metagenome)
  ORGANISM  Aminivibrio sp.
            Bacteria; Synergistota; Synergistia; Synergistales;
            Aminobacteriaceae; Aminivibrio.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,603
            CDSs (total)                      :: 2,582
            Genes (coding)                    :: 2,570
            CDSs (with protein)               :: 2,570
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 5 of 12
            Pseudo Genes (incomplete)         :: 5 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Aminivibrio sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185484_length_1001_cov_2.006342"
                     /isolate="AMR_MDS_3060"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872489"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..>1001
                     /locus_tag="P5219_13015"
     CDS             8..>1001
                     /locus_tag="P5219_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRX27736.1"
                     /translation="MVLAGLFAGAAFAAYPTRPFECIAPAGPGGGWDTTMRMTTKVLT
                     EQKFVTVPMPVINKPGGGGGVALSYLQKKKGKDDTLVVYSPPLLLINLTGSTPLSYKN
                     VTPISMLINDFGAYAVPKNSKYKNIKEVMEAIKKDPKSVKVGGMSAMGSMDHIQFLVA
                     AKAAGVKDLKAIQYIAFQELKRTVSDNKAQSGFVIITNPSTGEILALASYPSFDPNRG
                     SYRHLEGHTNMAVTHAFEPGSVIKPLWVAWGLEKKAFNMSQSVFCENGSYTFHRVTIH
                     DHEKYGWLPVSDIIKYSSNIGIAKLMDPIRSSDMYTCLQAFGMLEPTGISMPKAW"
BASE COUNT          219 a          336 c          277 g          169 t
ORIGIN      
        1 ggcttcactg gtcctggccg gcctgttcgc gggcgcggcc ttcgcggcct atcccacgag
       61 gcctttcgaa tgcatcgccc cggccggccc cggcggcggc tgggacacca cgatgcgcat
      121 gaccacgaag gtcctcacgg agcagaaatt cgtgacggtt cccatgccgg tcatcaacaa
      181 gccgggcggc ggcggcggcg tggccctttc ctacctgcag aagaaaaagg gcaaggacga
      241 cactctcgtc gtctattccc cgcctctcct cctgatcaac ctgaccggct ccaccccgct
      301 gagctacaag aacgtgaccc cgatctcgat gctgatcaac gatttcggag cctacgcggt
      361 ccccaagaac tccaagtaca agaacatcaa ggaagtcatg gaagcgatca agaaggatcc
      421 caagagcgtg aaggtcggcg gcatgtcggc catgggcagc atggaccata tccagttcct
      481 cgtggcggcc aaggcagccg gcgtgaagga ccttaaggcc atccagtaca tcgccttcca
      541 ggagctgaag aggaccgtgt ccgacaacaa ggcccaatcc ggcttcgtca tcatcacgaa
      601 tccctccacg ggtgagatcc tggccctggc ttcctatccc agcttcgatc ccaacagggg
      661 cagctaccgc cacctggagg ggcataccaa catggccgtc acccatgcgt tcgagccggg
      721 ctccgtgatc aagcccctgt gggtagcctg gggcctggag aagaaggcct tcaacatgtc
      781 ccagagcgtc ttctgcgaga acggctccta caccttccac agggtgacca ttcatgacca
      841 cgaaaaatac ggctggctcc cggtgagcga catcatcaaa tacagcagca acatcggcat
      901 cgccaagctc atggacccca tccgctccag cgatatgtac acctgcctcc aggccttcgg
      961 catgctggag cccacgggca tcagcatgcc gaaggcctgg a
//
